LOCUS       DAXWLF010000001        67412 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXWLF010000001 DAXWLF010000000
VERSION     DAXWLF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.001"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(38..1339)
                     /locus_tag="ACGE4U_000001"
     CDS             complement(38..1339)
                     /locus_tag="ACGE4U_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8392205.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     misc_binding    1602..1807
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1919..3175
                     /gene="tyrS"
                     /locus_tag="ACGE4U_000002"
     CDS             1919..3175
                     /gene="tyrS"
                     /locus_tag="ACGE4U_000002"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFF8392206.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            3466..5343
                     /gene="tdc"
                     /locus_tag="ACGE4U_000003"
     CDS             3466..5343
                     /gene="tdc"
                     /locus_tag="ACGE4U_000003"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFF8392207.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            5586..7007
                     /gene="tyrP"
                     /locus_tag="ACGE4U_000004"
     CDS             5586..7007
                     /gene="tyrP"
                     /locus_tag="ACGE4U_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFF8392208.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            7116..8483
                     /gene="nhaC"
                     /locus_tag="ACGE4U_000005"
     CDS             7116..8483
                     /gene="nhaC"
                     /locus_tag="ACGE4U_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFF8392209.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(8764..9783)
                     /locus_tag="ACGE4U_000006"
     CDS             complement(8764..9783)
                     /locus_tag="ACGE4U_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8392210.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(9805..10812)
                     /locus_tag="ACGE4U_000007"
     CDS             complement(9805..10812)
                     /locus_tag="ACGE4U_000007"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8392211.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(10805..11470)
                     /gene="pgmB"
                     /locus_tag="ACGE4U_000008"
     CDS             complement(10805..11470)
                     /gene="pgmB"
                     /locus_tag="ACGE4U_000008"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFF8392212.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(11463..13757)
                     /locus_tag="ACGE4U_000009"
     CDS             complement(11463..13757)
                     /locus_tag="ACGE4U_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFF8392213.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14017..16182
                     /locus_tag="ACGE4U_000010"
     CDS             14017..16182
                     /locus_tag="ACGE4U_000010"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFF8392214.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            16486..17319
                     /locus_tag="ACGE4U_000011"
     CDS             16486..17319
                     /locus_tag="ACGE4U_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFF8392215.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(17421..19121)
                     /locus_tag="ACGE4U_000012"
     CDS             complement(17421..19121)
                     /locus_tag="ACGE4U_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392216.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLHA
                     "
     gene            19628..20788
                     /locus_tag="ACGE4U_000013"
     CDS             19628..20788
                     /locus_tag="ACGE4U_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8392217.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            20804..21247
                     /locus_tag="ACGE4U_000014"
     CDS             20804..21247
                     /locus_tag="ACGE4U_000014"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8392218.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(21730..22260)
                     /locus_tag="ACGE4U_000015"
     CDS             complement(21730..22260)
                     /locus_tag="ACGE4U_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFF8392219.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            22365..22916
                     /locus_tag="ACGE4U_000016"
     CDS             22365..22916
                     /locus_tag="ACGE4U_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8392220.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(23069..23542)
                     /locus_tag="ACGE4U_000017"
     CDS             complement(23069..23542)
                     /locus_tag="ACGE4U_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8392221.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            23680..25152
                     /locus_tag="ACGE4U_000018"
     CDS             23680..25152
                     /locus_tag="ACGE4U_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFF8392222.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            25149..25859
                     /locus_tag="ACGE4U_000019"
     CDS             25149..25859
                     /locus_tag="ACGE4U_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFF8392223.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            26211..26495
                     /locus_tag="ACGE4U_000020"
     CDS             26211..26495
                     /locus_tag="ACGE4U_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFF8392224.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(26776..27195)
                     /locus_tag="ACGE4U_000021"
     CDS             complement(26776..27195)
                     /locus_tag="ACGE4U_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFF8392225.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(27197..27781)
                     /locus_tag="ACGE4U_000022"
     CDS             complement(27197..27781)
                     /locus_tag="ACGE4U_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFF8392226.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(27828..29303)
                     /locus_tag="ACGE4U_000023"
     CDS             complement(27828..29303)
                     /locus_tag="ACGE4U_000023"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFF8392227.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            29484..29795
                     /locus_tag="ACGE4U_000024"
     CDS             29484..29795
                     /locus_tag="ACGE4U_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392228.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(29911..32247)
                     /locus_tag="ACGE4U_000025"
     CDS             complement(29911..32247)
                     /locus_tag="ACGE4U_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFF8392229.1"
                     /translation="MLQLKDIKKYYKVGEPTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            32389..33057
                     /locus_tag="ACGE4U_000026"
     CDS             32389..33057
                     /locus_tag="ACGE4U_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8392230.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            33060..34103
                     /locus_tag="ACGE4U_000027"
     CDS             33060..34103
                     /locus_tag="ACGE4U_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8392231.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34340..35221)
                     /locus_tag="ACGE4U_000028"
     CDS             complement(34340..35221)
                     /locus_tag="ACGE4U_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8392232.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            35380..36108
                     /locus_tag="ACGE4U_000029"
     CDS             35380..36108
                     /locus_tag="ACGE4U_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFF8392233.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            36264..36581
                     /locus_tag="ACGE4U_000030"
     CDS             36264..36581
                     /locus_tag="ACGE4U_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8392234.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            36574..37182
                     /locus_tag="ACGE4U_000031"
     CDS             36574..37182
                     /locus_tag="ACGE4U_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFF8392235.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            37237..38028
                     /locus_tag="ACGE4U_000032"
     CDS             37237..38028
                     /locus_tag="ACGE4U_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFF8392236.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            38166..38654
                     /locus_tag="ACGE4U_000033"
     CDS             38166..38654
                     /locus_tag="ACGE4U_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFF8392237.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            38705..39577
                     /locus_tag="ACGE4U_000034"
     CDS             38705..39577
                     /locus_tag="ACGE4U_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFF8392238.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            39753..40115
                     /locus_tag="ACGE4U_000035"
     CDS             39753..40115
                     /locus_tag="ACGE4U_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8392239.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            40124..40876
                     /locus_tag="ACGE4U_000036"
     CDS             40124..40876
                     /locus_tag="ACGE4U_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8392240.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            40890..42692
                     /locus_tag="ACGE4U_000037"
     CDS             40890..42692
                     /locus_tag="ACGE4U_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8392241.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            42969..44009
                     /locus_tag="ACGE4U_000038"
     CDS             42969..44009
                     /locus_tag="ACGE4U_000038"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8392242.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(44061..46205)
                     /locus_tag="ACGE4U_000039"
     CDS             complement(44061..46205)
                     /locus_tag="ACGE4U_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8392243.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(46562..47935)
                     /locus_tag="ACGE4U_000040"
     CDS             complement(46562..47935)
                     /locus_tag="ACGE4U_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8392244.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(47935..48330)
                     /gene="rbsD"
                     /locus_tag="ACGE4U_000041"
     CDS             complement(47935..48330)
                     /gene="rbsD"
                     /locus_tag="ACGE4U_000041"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFF8392245.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(48358..49275)
                     /gene="rbsK"
                     /locus_tag="ACGE4U_000042"
     CDS             complement(48358..49275)
                     /gene="rbsK"
                     /locus_tag="ACGE4U_000042"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFF8392246.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(49383..50396)
                     /locus_tag="ACGE4U_000043"
     CDS             complement(49383..50396)
                     /locus_tag="ACGE4U_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFF8392247.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(50477..51076)
                     /locus_tag="ACGE4U_000044"
     CDS             complement(50477..51076)
                     /locus_tag="ACGE4U_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFF8392248.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            51262..51963
                     /locus_tag="ACGE4U_000045"
     CDS             51262..51963
                     /locus_tag="ACGE4U_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8392249.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            51973..54684
                     /locus_tag="ACGE4U_000046"
     CDS             51973..54684
                     /locus_tag="ACGE4U_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFF8392250.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            55135..56763
                     /gene="treC"
                     /locus_tag="ACGE4U_000047"
     CDS             55135..56763
                     /gene="treC"
                     /locus_tag="ACGE4U_000047"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFF8392251.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(56823..58004)
                     /locus_tag="ACGE4U_000048"
     CDS             complement(56823..58004)
                     /locus_tag="ACGE4U_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8392252.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            58256..59779
                     /locus_tag="ACGE4U_000049"
     CDS             58256..59779
                     /locus_tag="ACGE4U_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFF8392253.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(59848..60575)
                     /locus_tag="ACGE4U_000050"
                     /pseudo
     CDS             complement(59848..60575)
                     /locus_tag="ACGE4U_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
     gene            complement(60588..61868)
                     /locus_tag="ACGE4U_000051"
     CDS             complement(60588..61868)
                     /locus_tag="ACGE4U_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFF8392254.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(62009..62611)
                     /locus_tag="ACGE4U_000052"
     CDS             complement(62009..62611)
                     /locus_tag="ACGE4U_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFF8392255.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            62856..63062
                     /locus_tag="ACGE4U_000053"
     CDS             62856..63062
                     /locus_tag="ACGE4U_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392256.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            63374..64093
                     /locus_tag="ACGE4U_000054"
     CDS             63374..64093
                     /locus_tag="ACGE4U_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8392257.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(64174..64857)
                     /locus_tag="ACGE4U_000055"
     CDS             complement(64174..64857)
                     /locus_tag="ACGE4U_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFF8392258.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            65075..67261
                     /locus_tag="ACGE4U_000056"
     CDS             65075..67261
                     /locus_tag="ACGE4U_000056"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFF8392259.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        21482 a        12023 c        13664 g        20243 t
ORIGIN      
        1 gtcagttcgg cttttttggt tcataaatga cgtaaaatca agcagattcg cgtgcttctt
       61 tgtcatagac atcatggaca ttgatgaatg gatcaagaat aggatttttt ccgtctaaat
      121 cagattgaaa acgaatgcca tcgatattga tccgagcatt tcgatagaaa tccgtaccta
      181 attgtcccca agctggtgta gaagcatcga cattacagaa aatatcgaaa ccttgactct
      241 tcaagtaagc aaatttttga ttttctgctg aataaggctg ccagtcgctg atgtctgcac
      301 caaatggata aatcagaata tttgtttttc ctaggatagg tgcaacttct tgctgccaca
      361 attcattgtc tttttttaca tcttctagtg aagcttgagt catattcaag tgaccccatg
      421 tatgggaagc aaagctccag ccgtctttct tcaactgttc cgccactttt ttagcttctt
      481 taatctcttt ttttgttttt tcattgtctc catattgaga ttttgatgtt cgataaccta
      541 aaactccgtt gtaacctgtc agggccaagg taccttttgc gcctcgataa gaaaaatcag
      601 gatgtttctt gacaaaggca tcgatcagag gaaccatgtc aaagtcaccg atttcttctt
      661 tatctttctc tttatagaca tttttgattt cattgttatt tgatacgacc aatcggctag
      721 gaaaaccgga attatccata tattcataat agctgacatc atcttgtgaa agaaccaatg
      781 gtttttttcc ttcaggtagc gcaagcccgt taaaggaaag atttccttgt tcgtcttttt
      841 ggatcaaatc attcaaatta actaaaacaa aatcattttg ataaagttgt tcgatcgaac
      901 ggttgaattc ttcaacagtc accatataat ctttgtatcc ttgtgcctgt tgagagtgga
      961 aggctttttg cgggtccacg atcaagctgt ggaaaaagac atgagagatc tgagtaggat
     1021 cagaccagtc gatcagttgt tctttctcct tttttagtgt ttgaagaagc tgttgtgttt
     1081 cagtagatcc gttcttacta tctttttcca ataacgcaat cgcttggtca taatcgtagc
     1141 tttgagccag attttttgct tgagtaacta gttgttgctt atcatctgtc ggttcttttg
     1201 ctttttctgt tatttttttt tcaggaatga ttggttcttt ttttgtagat gatcggatga
     1261 caaaaaacaa acttagaatt aaaataacga tagttaaagc agaagtggct cctaaaataa
     1321 ttttttgctt tttttccata tacctcgtcc cttgcttttt cattttttta cttgtcccat
     1381 tccttattgt aacgaagaaa gaataagagg caaagtaaag tgacttctcg ataaattgca
     1441 gaaaaaatac agaaaaagtt atttctttta acaataattg aaaataaaat gttcggaatt
     1501 agagcggatt caaaaaagaa aaaatgagga atttaaaaaa aatatccttt ttctgttgac
     1561 aaagaaaaag ttgcgctcta tgatagacac atattaataa taacaagacg aagatcgaaa
     1621 cgaagtagtt gttaaagcca atgtttcaga aagctggtgg tcgctgcaaa ccagtacatg
     1681 ctttaaagcg aattacattt caggagtctt tggcagtgat tgccacgcga cagcgttaaa
     1741 tgttcgagag gtatgttgga tatctagaca tacaaattgg gtggtaccgc gagatttcgt
     1801 cccttggctt atatgccaag ggactttttt tatttcctat cctatcctga agaaataatt
     1861 aattacacat gctagcaggt tcctgctttc ataaaaaaac tacacggagg tcagtaaaat
     1921 gaatattatt gatgaattaa cttggcgcga cgccattaat cagcaaacaa acgaagaaag
     1981 acttcgcgag ctcgttgaag aaaagagcat ttcactttat tgcggcgttg atccaactgg
     2041 tgattcgatg catatcggac atttgattcc atttatgatg atgaaaagat tccaattggc
     2101 aggccatcat ccatatattc tgattggcgg cggaacagga acgatcggcg acccaagcgg
     2161 ccgcaaaaca gaacgtgtgt tgcaaacgat ggaacaagtg caacataacg tggacgcact
     2221 ttccaatcaa atgagaaaat tgtttggtaa agatgcaaat atcacatttg tgaacaacta
     2281 cgactggttg tcaaaaatct ctttacttga atttttgaga gactacggta aaaacttcaa
     2341 catcaacacg atgttagcaa aagatatcgt tgccagccgt ttggaagtag gaatctcatt
     2401 tacagagttt acttaccaaa tcctgcaatc tatcgacttt ttgcatctgc ataaaacata
     2461 cgatgtgcaa ttgcaaatcg gtggagcaga ccaatgggga aatatcacag ctggattaga
     2521 cctaatccga aaattagaag gaccagaagc agaagcgttc ggtttaacga tcccattgat
     2581 gctgaaggct gacggaacga aattcggaaa aactgcaggc ggtgccgttt ggcttgatcc
     2641 gaagaaaact tcaccatttg agttctacca attctggttg aatcaagatg atcgcgatgt
     2701 agtgaaatac ttgaaattct ttactttctt gtctcaagaa gaaatcgaag acttagcgaa
     2761 aaaagtagag acagaacctg agaaacgtga agcacaacgc cgtttggcag aagaagttac
     2821 aagatttgtg catagtgaag aagacttgaa agaagcgcaa aaaataacgc aagcattgtt
     2881 ctctggaaat attaaagaac taaatgcgga agaaatcgcg caaggattcg gtaaaatgcc
     2941 gaacgttgaa atttcaagca cacctgaaaa tatcgtggaa ctgcttgttt ccacaaaaat
     3001 tgaaccatct aaacgtcaag cacgtgaaga tgtttcaaac ggagctataa gtattaacgg
     3061 tgaccgcgtt accgatttaa attttgtcat aaatccatcc gatgaattcg atggtaagtt
     3121 tgtggttatt cgaaaaggta agaaaaatta ctttttggcc aaagtaattg attagaattt
     3181 agtaagtttt taccttgaaa aagggaaata ctattacaac attccaattt tgaaacccat
     3241 cctaaaatag aacgctggtt gtcgttaata caatctttgc gagggagttt acatttgtaa
     3301 attccctcgt tttttttaat tatttcaaaa gtgtgaacaa ttcggataat tgatacatat
     3361 taaaatatga ttaaaaatca ggccaaaact tagtttacag aatagaagaa caggtgtaat
     3421 gtagggctcg acaaaattta aatatttttt taggagggtt tcactatgag tgaatcattg
     3481 tcgaaagatc taaacctaaa tgccctattc attggggaca aagctgagaa cggccaaatc
     3541 tataaagctt tgttgaatga attggtggat gaacatttag gctggcgtca aaactacatg
     3601 cctcaagaca tgcctattat tacgccagaa gaaaaaagca gtgctagttt tgaacacact
     3661 gtgaataaaa caaaggatgt tctatcagaa atttcagcgc ggatgcgtac ccattccgtt
     3721 ccttggcata atgccggacg ttactggggt catatgaact ctgaaacatt gatgccatct
     3781 ttactagcgt acaattttgc tatgctatgg aatggtaata acgttgccta tgaatcttct
     3841 cctgcaacaa gccaaatgga agaagaagta ggaatggaat ttgcaaaatt aatgagctat
     3901 aaagatggct ggggccacat cgttgctgat ggttctttag caaacttaga aggactttgg
     3961 tatgcccgca acatcaaatc attgccgctt gcaatgaaag aagtgacacc tgaactagtt
     4021 gctggaaaaa gcgattggga attaatgaac ttgtcaacag aagaaatcat gaatctgtta
     4081 gacagcgtcc cagaaaaaat cgacgaaatc aaagctcact cagcacgtag cggaaaacat
     4141 ttagaaaaat taggaaaatg gttggttcca caaactaaac actattcatg gctaaaagct
     4201 gccgacatca tcggtatcgg tttagaccaa gtgattccag tgccagttga ccacaactat
     4261 cgcatggaca tcaacgaact tgaaaaaatc gttcgtggtc tagcagctga aaaaacacca
     4321 atcctaggtg tagtcggtgt tgtaggatca acagaagaag gagcaatcga cggtatcgat
     4381 aaaatcgttg cattgcgtcg cgtattagaa aaagacggta tttacttcta cttacacgta
     4441 gatgctgctt atggcggata cggccgtgcg attttcttag acgaagacaa caacttcatt
     4501 ccatttgaag atttaaaaga tgttcattac aaatacaatg tctttacaga aaacaaagat
     4561 tatatcttag aagaagtaca cagtgcatat aaagcaatcg aagaagcaga atctgtaacg
     4621 attgacccac ataaaatggg ctatgttcca tattctgccg gtggtatcgt catcaaagat
     4681 atccgcatga gagacgttat ctcttacttt gcgacatatg tatttgaaaa aggcgcagat
     4741 atcccagcat tacttggggc ttatatcttg gaaggttcaa aagcaggggc aacagcagcc
     4801 agcgtttggg cagcacacca tgtcttacct ttgaatgtca caggatatgg taaattaatg
     4861 ggtgcatcaa tcgaaggtgc acatcgtttc tacaacttct tgaatgactt gtcatttaaa
     4921 gtaggagaca aagaaatcga agttcatcca ttaacttatc cagatttcaa tatggtagac
     4981 tatgtattca aagaaaaagg caacgatgac ttagttgcaa tgaataaatt gaaccatgac
     5041 gtatatgatt attcttcata cgtgaaagga agcatttacg gtaatgagtt cttaacttca
     5101 catactgact ttgctatccc agactatggc aacagcccat tacaatttgt gaaccaacta
     5161 ggattctcag atgaagaatg gaaccgtgcc ggaaaagtaa ctgtattgcg tgccagcgta
     5221 atgacaccat acatgaacaa agaagaacac tttgaagaat atgctgaaaa aatcaaagca
     5281 gctcttcaag aaaaattgga aaaaatctac gcagaccaat tattggcaag cgaagcaaaa
     5341 taagcaaggt aatcatgtta gaaaacagct tgaatactaa ctgtttctga cgcatgtttg
     5401 gatctgaaga gcaaggggag acctaagctc ttcgatccct ttgttttgag gttgtctttg
     5461 aagaaaccct tcaagcaata aactcaaaga atcccctcaa atattcgctg aattgtttcg
     5521 tttattgctg aaactacttc aaagacaccg ttattttatc caaaaaatca aggagagtgt
     5581 caacggtgag taaatctgaa gtaagtaaaa tgggtcttgg gacctttatc ggtctgacaa
     5641 tggcactgtg tgcaacggtt cggagtattc ctacgttagc cgcagtcggt tggacattga
     5701 ttttttattc aatttttgca gttttgtttt ttgccggtcc gatatcaatg gtttccggtg
     5761 agctttccac gatgcttcct caagaaggcg gtcctcagtt atgggtaaaa acagcactgg
     5821 gcagcaaatg gggatttgtc gttgcatggc tattatgggt gcaaatgttc ccaggtatgg
     5881 taatggtcgc ttctacttta ggaccattgt taggaaacac atttggtaat gtagaacttg
     5941 gcaataatca ctggttcgtt ttaggttgta ttttagttat ctactggatc atcacgatct
     6001 tgaacttgaa attcgatatg gcaaaagtcg gcgggaacat cggtgtatgg ctaggggtat
     6061 atatcccagt agtcatcatg ttcgttttag gtgttttggc agccttcaaa gtcggactag
     6121 tctcaaacgg ctatctaggt gacttttcat ggtcaaaagc tttccctgac ttggaacata
     6181 ttgattcatt gaaatactta gctggtatca catttatttt tgttgggatc gaaatgagtt
     6241 ctgtttacat gcctcgtttg aaagacgcaa caaaaaatta tacaaaaggc gtatttatcg
     6301 ctttgatcgg attagtttta ttgaatgtca tcaatgcgat gcttgtagca aatgtcgtac
     6361 cagatggaaa aatggaatta gcaaatatca cgcagcctat cttgattgac tgtcaaatcc
     6421 taggcttacc agaagtgatc ggaaatatct tcagcttcat ggtatttatt ggtgtgcttt
     6481 tacaattatc agcttgggtt actggaccat caaaaacaat catccaagta gctcgtgaag
     6541 gattccttcc accaaaattc ggattccata aagaaaacaa atacggcgta tctagaaatg
     6601 tcgttctaac acaatcaatc gttatttcat tatttgcttt gctttatggt gtaatggatg
     6661 acgtaagtgc tgtattcttg acacttacaa atgctacaac tgttatttat tgtatcgtgt
     6721 acattttgat tgctgtttca ttgcttaaaa tgcgtaagaa acatccagaa ttcgaacgtc
     6781 cttaccgaat tggtaaaaac ggaaacggat tagcttgggt agtcagctgc atgctgatct
     6841 tctctatcat cgttgtcgta tttgctacat taggtacagc gacattgagt gatgcattat
     6901 tagtagcagc tatcacagtg gtcatgttcg ttattccatt gatcatcaat cacttcaaga
     6961 aagattcttg ggggattgag gtagaaaaaa gtttagaaga aaaataaaaa aataagtctt
     7021 tcagccgacg ttttgtcgct gatttagtag gaggtcctgc caagtgaata gttttttcat
     7081 ggcagggcct tttcatttga aataggagat cgacgatgaa taaaaatatt tctttaaaag
     7141 aaagtatcgc tgtattgatc gtgttgttgg cactcctagg agtactgatc atcggattcc
     7201 agctctcgcc acatatcccg atccttgcag ctttcatgtg tttgttgttt tatggcagat
     7261 ttaaaaagtt cacatgggat gagatccatg acgggatcat caaaggaatc actccaggga
     7321 tcattccaat cctgattttt cttttgattg gtgtcttagt tgcttcatgg attttatctg
     7381 gaaccatccc tacgattatg gtttatggat ttaaacttgt ttctgtgcgt ttctttttac
     7441 cgacagcatt tcttgtttgt gcaattgtcg gagtgactgt cggcagttca tttacaacga
     7501 tttcgacgat tggaattgct tttttgggga tcggtcattt acttggattc aatgatgcaa
     7561 tgactacagg cgcagtcgtt tccggtgcct ttttagggaa taactgctcg cctttatcag
     7621 atacgaccaa cttggcagcg ggtattggag gagttaatct attcgaacat atcgctggta
     7681 tgcgttatac ggatttccca gcattcgtcc tttcgttgat tttttatacg atattaggac
     7741 ataacagtca ttcagccgat ctagctagta tcaatgaaat gatccaaaac atgaaagcga
     7801 gcttttggat ctctccttgg acattgattc cactagttat tttatttggg ggggcaatca
     7861 aaaaaattcc ggctatccct acattactag cgggatcgac agctgctctc gttctttctt
     7921 ttatccatga gaaaagtttg acggtcggaa aagcagcaga catcttgatg aatggttatg
     7981 tcgcaaatac acctgatcca aagctagatg aactgctttc tcgaggcggg atcatgagta
     8041 tgttaggttc tgctagtttg attttacttg cattggcatt aggtgggtta ctgatcaaat
     8101 atctgatcgt tgataccatc gttcaagaat taaaagaaaa gatggaccga ccttctcgtt
     8161 tagtcgggtt tactgccttg agctgtatcg gaatcaatct gatcgttgga gaacagtatc
     8221 tgtccatcat tcttccagga gaaacattca agcgttcttt tgatgacgtt ggcttatcaa
     8281 aaacctattt gacacgaaca ctagcggatt caggtgcgac cgtcaattct ctcgtaccgt
     8341 ggggagtcag cggcacattc attatgggaa cgatgaaagt tagcgcgttg cattacttgc
     8401 cattcgcctt tttctcgatt cttgcacctg tctttacaat attaggcggt tttttattga
     8461 atcgtcgacg tgtaaaaaat tgaaggaagg aaaaaagagg ctgggacaga agcgtttaac
     8521 tccaagaaat aagaagaaac tcaagaaaat tgctttttaa atttttgtga atttcagctt
     8581 atttccgaag gagttgcttc tgcttccgcc gtttatacgt gtttagagcg tgagacaaaa
     8641 gtgttcttta cttttgtccc acgctctttt ttacagataa acaatgtgtt agaagtagta
     8701 tcttctttta cataaaaagc tgaccatagg ataaatggtc agcgtattta gtgagtcagc
     8761 tatttagtat gggttttcta aaggaacgac tgtctctctt ttgatcaagc gatgagggat
     8821 gaccaaacgt acgccactgc tcaattcttc atggatcaat tcgcgcagct tttgcatagc
     8881 gacacgtcct aattcggaga tatcgatatc gatacttgta aggtaagggt gtgttaatgt
     8941 tgagaagatc gagttgttga agctaatgac ggagagtgtc tctggaatga catagccata
     9001 catttgcgcc aactgcatcg tgcgaagggc gaagatatcg tcaatgacca ccaatgctgt
     9061 ggcttcgttt tctttcaacg ttttctcaaa ggacagataa tcctccggat gctctagtac
     9121 tacactggat aacgcaggaa gaccagccat catcattgct ttttgataac cgaaatatcg
     9181 ttcgaaatac aaactttcat gagtcgtgtt tgtcacaaac aggatattct tatgtccatt
     9241 ctggatcaaa aattctgttg cttgtttacc tagaagctgg ttatcattat ctacataaac
     9301 gatttcgttc tcatgatgat aaggctgtcc gatcaaagtg aaagggatgt ggttttggta
     9361 taaataatca atgacagtat cttgactatc tgaatacact agaataaaac catctacttg
     9421 tttttgtaaa tgcatacgct ggacgttctc taacagcaat tcaaaatttt tagcagtagc
     9481 gattgctgta gtcatatcat aattccgcgc ttcttcgttg attgcttcca tgatctcaag
     9541 ataaaatggg ttaccgatcc gctcttttga atcaagaggg ggtaagatta cgccgatggc
     9601 actggaaatg cgttttccta gattgcgggc agtcatgtta ggaacataac ctagttcatc
     9661 cataattttt cggactttct tctttgttgt ctcggaaata ctaggatgat cgttgatgac
     9721 acgagagacg gtcgaggtgg caacaccggc ttttttcgcc acatctttta cggtaatagc
     9781 catgataaaa acacctttct cttgttattg tgcagagaac cgataaatcg tctgactgat
     9841 ccgttcttga ccaggatgga ggttgatcga tccaaatttg gggaaatgaa cgatatcagg
     9901 gatttcttgc ggttcgattg ctaatccgta gtttgaatgc atctgttttc cattgacaga
     9961 gaaatcagct tcgaatccag tcgtagaaaa taataccata cttttattag aagttgctat
    10021 agtcatttgg cgaccagaat gcttctcgaa taattgtaag gaagttttgg aaagcctagg
    10081 atgatgcaaa agaaatacat catctagtcc atctggataa catgcaagga tctcttgcag
    10141 tgttggggat tttctaaagt cgattggcag tttttctttt tttgtccaat ttccagtagg
    10201 cagtttttct tgatctaatt caagcacacc atctaaaaag accgttaact gatgttcata
    10261 gatgtctctt gttccatttc ctgaaaggtt gaagtaggca tggttggtcg gattgcagat
    10321 ggtttcaaca aggctactag cagaggttgt catctctaat tcgttttttg ttaaacggta
    10381 agtgatcgta gcagtgattg gtcctggata ggtagaaaat tcatctttta agtaaaagac
    10441 cacacctatt gatgatggag ttttgaaaac ttctacttcc caaaattgga aagaccagcc
    10501 tttcgtgcca ccgtggatat ggtgtgatcc gcagtttttc tccaatctat gctcacccca
    10561 agaagcatcc cggatccttc cggctacagg tccgacagtt gctccaaaaa agcttttgtc
    10621 tgttagaaca tcttcgaaac gatcgtaaga caataaaaca ttttctgatt ttccttcttt
    10681 gtctggtgcg aagatttggt gcaggcgggc accgtaattg agaagaacga cttctaagta
    10741 gtcgttcttc aatgtgattt tctttatttc agtctctgga actggtacga cggagatctc
    10801 tggattatcc atggtcatgc cacttttctt tcaagtaatc gatcgtaaga aaagtcgtat
    10861 cagggacaag cgggatctcc tctccaaggt cttcagcacg tcccacgcca attggttgag
    10921 cgccacttga gaggatggct tggataccgg cttgcgcgtc ttcgatcccg atacattctt
    10981 ctgcttttag tccgacagct tttgctgcaa ggaggaagat atctggcgca ggttttccgt
    11041 ttgttgcttc tgccgggtcg gcaatagcat caaagtaagg tgtaagaccc attttttcaa
    11101 gtaggaatgg cccgttttta cttgcagaag caagagcaat cttgattttt tcttctttta
    11161 aagaatccag taaaggcacg accccgggga agacatcttt tggttcgatc gtctggatca
    11221 tctctaaata ataatcgttt tttcttttcg ctaattctgc aaattcttcc tttgaaaaag
    11281 agtgttcttt tccgccataa gctaaaagca aagagagtga gtcttctcgg ctgacgcctt
    11341 ttagctgttc gttgaatgtg tggtcgatcg taatgccgat ttcctgacca agctttcgcc
    11401 aagcacgata atggaattca gcagtatcgg taatgacacc gtctaaatca aacaataccc
    11461 ctttaaacat ttgcttcctc ctttcttagc gttatttcta gacgatccgc taaagaataa
    11521 gactgttcat agacgtaaat ttctagagga gaaccttcta ataactcgaa agtaacttgt
    11581 tcatctgtga ctgagatgaa caataaacgt ccgcgataat tgatatggaa agaataagct
    11641 ttccaagttt ttggcagaaa tggtgcgaat ctcaactgat catgggcagt tttcatttga
    11701 gcaaatcctt ggacaatcga caaccagcta ccagtcatgg aagtgatgtg caagccgtct
    11761 tctgtgtcgt tattgtaatt atccaaatcc aagcgagctg ttcgctggta catttctaca
    11821 gctttgcttt ccattcctaa ttctgctgct aaaatcgaat ggatacttgg cgaaagggaa
    11881 gactcatgga cagtcattgg ttcatagaaa tcaaagtttt tttgcttttc ttcttttgta
    11941 aaagtatcat tgaagaaata aatcccctgt agtacatctg cctgcttgat gaagcaagat
    12001 ctaaggattt tgtcccaaga ccaatgctga ttcagaggaa gatcgcttgt gggaatatcg
    12061 gatacaggca tgagatcttt atctaagaat gtgtcatgct ggacaaagat tcctaattct
    12121 tcgtcaactg ggaagtacat gttctctgcg atatctttcc atttggctag ttcttcttcg
    12181 ctgatagtga tagcagcttc tttttgataa gttaaataat tttctcgcgt gtaacgcaag
    12241 acccaagctg cgatcgtatt tgtataccaa ttgttattga tattgttctc atattcgttt
    12301 gggccggtca caccgtggat catgtattta tttgctcgtt ttgaaaaatg gacacggtct
    12361 gcccagaagc gtgcgatttc tgctaaaact tccagtcctt catttttcaa atattccgta
    12421 tcgccggtgt aattcgtata attgtagatt gcataggcaa tcgcgccatt tcggtgaatc
    12481 tcctcgaatg tgatttccca ctcgttatgg cattcgacac cagtgaaggt gaccatagga
    12541 taaagcgcgc cttttaatcc ttgttgctga gcgttgtgga ttgcttgcgg cagttgatta
    12601 tgccgatatt tcagcagatt cttagttact tcaggtttgg ctaaagcaag ataaagaggg
    12661 acagcatacg cttctgtatc ccagtaagtg gctccgccgt atttttctcc tgtgaatcct
    12721 ttggggccga tattcaatcg ttcatcttcg ccgtagtaag tagagaatag ttggaacaaa
    12781 ttgaatcgaa taccttgttg tgcttcttcg tctccttcga tgaccacatc ggcaagcttc
    12841 catcgttctg cccaagccgc tgtttgctct tcttttgctt ggatataatt gtttgtctgg
    12901 ttcgttaatt ctgagacagc tttttctgct tgctgcgctt ctggaatatc tctactagta
    12961 agaacgataa tttctttttc cactgaccat gtctcacctt gagataattc tgcagacaag
    13021 caaccatgta atgctaaagt atcttcgtga tagtcaggga tgactgattt tccattaacg
    13081 aaatggcgca ttgctgcagt aacagtaaat tgttcaatcc caaagttgtt tggaatagtt
    13141 ttagtcgtca aatagccgat ttcttctata gttccacgat caatctcatt ccaaaaatgt
    13201 tcatcatagt tgctgtcttc gttttgaaca tttccatcca attttgatac gagtttgact
    13261 gttccgctac cttcaagcat ttctatagtt aaatagatat aagctgattc ttttttgaca
    13321 atgctcagaa aacgttcgaa ggaaatgcgt actttattag tagaagtagt gattgtgaat
    13381 gtacgagata atacaccatt tttcatatct aattcccagt aaaagtcttc tggttccaaa
    13441 gaagccaagt cgatcagctg atcgttgatc gaaatatcta tcgcgataaa gttcaaggcg
    13501 ttgatcactt ttccaaagta ttctggataa ccatttttcc accagccgac acgtgtctta
    13561 tcgggatacc agacacccgc caaataagta ccttgatggt ggtcccctga gtaagtttct
    13621 tcaaagttcc cgcgcatccc catataaccg tttccaatac ttgttaaaga ttcttgcagt
    13681 cgaagatttt ttttatctaa ttgtgtcgtg ctgattttcc atggatcaat agcaaatagt
    13741 cgtttgatct gtttcatttt tctcctccta atgagctgtt ttatacgttc ataataaacg
    13801 caaccgattg cacaagtcaa tagacaaaag aaaatatttc atagaaaaaa gcttaaaaaa
    13861 ataaattttt aggagaaaat tatagaaggg aacacacgta ttataggttt tatagaatgg
    13921 tatagacaga aaaataaaaa ataaagcatt tacaaaaaag aaaacgcacg ctataatacg
    13981 tttaacgaaa ccgattgcgt aaaggagcta aacggaatga aaaagatatt tagttttgag
    14041 ttttggcaaa aatttggaaa ggctttaatg gttgtggtag cagttatgcc ggcagctggg
    14101 ttgatgatca gtatagggaa atcaattccc ttgatcaatc ctgattggac gccgcttgtc
    14161 acaacgggcg gagtaattga aaatattggt tgggcaatta ttggtaatct tcatatttta
    14221 tttgctttag caataggagg aagttgggca aaagaaagag caggcggagc atttgcagct
    14281 gggatctcat ttattttaat caatcggatc actggggcta tctttggtgt cacaagtgac
    14341 atgcttgcga atgaagatgc atttacccac actctgttcg gtacgaagat catggttaaa
    14401 ggatttttta caagtgtatt ggaagcacca gctttgaata tgggagtatt cgtagggatc
    14461 atcgctggat ttgtcggggc aatggcttat aataagtact ataattatag aaaattgccg
    14521 gatgctttgt catttttcaa tggaaagcgc tttgttccgt ttgttgttat cttatggtca
    14581 acaatcgtgg cattgattct tgctgtggtg tggccaaacg tacaggctgg aatcaataat
    14641 tttggtttat ggattgcaga atcacaagaa agcgcaccga ttctagcacc gtttttattt
    14701 ggaacattag aacgtttact attgccattt ggattgcatc atatgctgac gattcctatc
    14761 aactatacac agcttggcgg atcgtatgag attttatcag gcgcacaggc ggggacacaa
    14821 gtatttgggc aagatccgtt atggttggct tgggccactg atttagtgaa tcttaaaggt
    14881 gcaggggata tgtcgcaata tgagtttgtg ttgcataact ggacgccggc tcgtttcaaa
    14941 gtaggacaaa tgattggttc ttcaggtatc ttgatgggaa tggcttttgc gatgtatcgt
    15001 aatgtcgatc ctgataagaa agcaagatat aaatcaatgt atttttcagc agcgttggct
    15061 gtctttctaa caggtgtaac agaaccattg gagttcatgt ttatgtttgc tgctgtgcca
    15121 ttatatgtca tttattcagt gatccaaggg gctgcatttg cgatggcaga tatcttgcca
    15181 ttgcgggtcc attcatttgg gaatatcgaa ttgctgactc ggacaccgtt agctatcaaa
    15241 gcgggtctag gtggagatct tatcaacttc gtgttgtgtg tgatcgcttt tggtgttgtt
    15301 acgtatttct tagcaaattt cttgatcaaa aaattcaact ttgctacacc gggacgtaac
    15361 ggaaattacg acaatgacag tgaagaaacc gttgtatcta acagcggaac aggaacagca
    15421 gatcagcaag tggtccagat cattcatttg ctgggaggaa aagaaaatat caaagatgtc
    15481 gatgcttgta tgactcggtt acgcgtaagt gtaaatgatc gagaaaaagt agggactgaa
    15541 gaagcctgga aacgagcagg ggcgatggga ttgatcgtca aagacaatgg agtccaagca
    15601 gtttatggac caaaagcaga tgtcttgaaa tcagatatcg aagatctgct gcaatcagga
    15661 gcagagattc cagagccaga aatggtttct acaggaaatg gacaagcagg tagtaagcaa
    15721 tacttaggta tagaacaaga actagtttcc gcagcttctg gagaagtgat tcctttatct
    15781 gaagtaaaag atccggtatt ctcacaaaaa atgatgggtg acggctttgc tgttattcca
    15841 acatcaagag aagttgttgc gccgatcgct ggaaaagtga caagtatttt ccctagcaaa
    15901 catgcaattg ggctggaaac gaaagatggt atcgaagttt tgatccatat gggaatcgat
    15961 acagtccaaa tgaatcagcc agcttttgag attgctgtca aagaaggcca agaagtagga
    16021 acaggaacga aattagctga gatggatctt gatgtgatcc aaaacgaaga aaaagatcca
    16081 acgatcatga tcgtatttac aaacgataaa gtagaagaag tagtgatcaa gcaattaggt
    16141 acagtgacag ctggtaaagt gatcggctcc attaaaatgt aaggagaggt tgtgaaaaag
    16201 ctgtcctgca tcaagtcata agaacagcca aacaaaaata gctcctccta tttttcgttt
    16261 ggttgggctt attaccgaga agcagcagtt tgaatactgt ttagtggaga ggtcgtgaaa
    16321 aagctgccct gcaccaagtg ataaaaacag ccaaacaaaa aatagctcct ggtccattga
    16381 gcttattact gaggagttag caggagaaca ctatttgctg gagaggttgt gacaaaaatt
    16441 ttgtcacaac ccttttgttt cgaaatgaag acaaggagaa aaccaatgaa attcatgaca
    16501 ctcaacacgc atagctggct tgagccagaa ccagagaaaa aattgcaaga attagcagat
    16561 aagatcctgt tagaagatta tgaagtgatt gctttacaag aaatcaacca attactggaa
    16621 tctgaagaag tagaacctgc gaagctaatg aagttctgtc cggtaaagaa tcaagtacct
    16681 atccgtaaag ataattttgc ctaccgttta gttcagcttt taaaggaaca cggaaaagag
    16741 tatttttgga gttgggaaat gagccacatc ggttatgata aatatgaaga aggaaatgca
    16801 ctgcttacga aaaaaacatt ggaaagtcag gtccttaccg tctcccaatt tcaaaagaaa
    16861 gaagattatc agacacgcaa gatcttgatt gggaaaacaa agatagatga tcaggacatt
    16921 tttgtctcga gctgccactt ttcttggtgg acagataaaa aaagtggctt ttattttgag
    16981 tggaaaaact tggaaaacta ctttttggaa actcgcgtac cacttttttt cctaggtgat
    17041 ttcaataatc cagtagactc tcaaggttat tatactgtaa gagaaagttg ccttcttctt
    17101 caagattcgt atgttgtcgc gaatgaaaaa ggaaaagcag ctactgtgga aaagaaaata
    17161 gatggttggg aacaaaatac agaaaaattg cgtatagatt ttattttcgt ccctgaagga
    17221 atgcaggtta aaaaatatca acggattttt gatgggatcg atagtccaat catcagtgat
    17281 cactacgggg tggaaatcga attagatgtg aacgaataaa aaatataaca tataacataa
    17341 aaaaactgat taaaataaaa ctctccaaac gattataccg tttggagagt tttttgatcg
    17401 gatgtattta tcccttttgc ctaagcatgt aaaagcttgt atctatgata aataaataaa
    17461 catagcaagg taaaccagcc aatcaaagta agagaaagaa tgaaatagaa atagattggc
    17521 gtttgataat gaagcgtgag ctcgttgatt cctttttgct gcttcacgat tggggtcccg
    17581 atatctgtta cttcataatc agtcaatgtt tttccatttt gctgtagcac agtccgatcg
    17641 tagacgataa cagggatatg aacagttttg tccccagtgt ttttccactt gattgtcaaa
    17701 agaccgcctg gatgtgtttt tataaaagtt gtttcgtttt ctagtatttc ttcgttatac
    17761 cggtcgtatt tattgtcttt tgtctcctta tagattggca gatagtcagg tgtggttttt
    17821 tggaaaacga acaagctggt agacatatct ttgctgaaga atgttttcct tgcttcttcc
    17881 ggcgtatcga atagataagt atgtcgactg acaaatgttt cattttccca cttttccaga
    17941 tgctggtata agttctggat cgtctgtgct gaaaaaataa gaatgccaaa tatacttaaa
    18001 agacggaacc acttttgttc agaccactgg ttcagtgaaa gggcaaggta aagtagaagc
    18061 aatagcgtaa atggcacgaa aaatcgaaat ggaaactgga tcaaactaat aaaaggcaca
    18121 tttttttcga tcaatgtcgt ccatggaatc aagctagtag aaagaaaaaa gaaaacaaga
    18181 cttgtgtaca acacgatttt ttggaataag gtgttctttt gttgaaagaa aataaaaagc
    18241 aaaaaacctg ataatatcaa tataggaaag gccttagggt aatggagcca ataactgcca
    18301 tccagatcta tcgccttttg actcatttct ctgttaacaa agggaggaag aatcttattc
    18361 ccactgtaaa caatagctaa tcctgcccaa atatttgctg tcagaacaaa ataaagcaag
    18421 accgacttaa tcagttgaag cagtgctttt cctttttcag tctggttaaa aaagtaataa
    18481 agataaaaag ggatatacat caaagctaac atgatcgaag acagtaaatg gatctgtgtc
    18541 ataagtccca ctgagacagc gactttgata acagggaact ttttatgaag gacgaaatca
    18601 gcaataggga tcatacataa aggataaaaa caagtccccc agctagtgaa tccttggttg
    18661 atagaccagt attgaataga atacgtactc atgaataaaa cggctactgg tacacttacc
    18721 caatttcgaa cattgacata tctcagtaaa atcagcatag aaaatcctga aataagataa
    18781 agaatgaaat ttgaaagaat ctgataatga aaccagctac ctgcaagcaa cacgatcaat
    18841 ccttgtaaat aagcgaaaaa tgggccgtaa acggcattca cgatcctgcc actttgctgg
    18901 aatccataaa gggaaggaaa ataatggaaa tccccatatt taatttgttg cgcggcatca
    18961 tagaaccgat tgtaatggaa tggggcatca gcacccaaaa ttagtttatg tgtgaataaa
    19021 tgaggcatga gaaatgccaa agcaatgaaa aaaattgcta aaaatgggcc atattttttc
    19081 aattgtcgaa ctcctttgca aactttagat aaattcatca tatatcctat gcgagaggta
    19141 ggcaagaaat tagaaaattc atgaaaatat ttatgaattt tacaagaaaa acataagtga
    19201 aagttctaag gagagaggat gcgacataag ttttgttgca tctccttgtt ttgaaacaga
    19261 atttttctta atactcgaaa caaagtagct ttttcatgac ctctcggtaa acggtgttca
    19321 atcagctgct tctcggtaat aagctcacta gcctagaaaa tatgagaagc tattttctga
    19381 ctagcgctct tattactcaa agcataacgc agttttcaca acctcttcct taaacggtgt
    19441 tcaaaagctt gcatctgcgg taataagccc aacccaacaa aaaatatgag tagctatttt
    19501 tttgggctgt acttattact tgatgcagga cagctttttc acaacctcat cggctttcat
    19561 tttctatttt tttcctgaac ccatataata aaagaaagaa caaaaaaact tgcaagggag
    19621 gacacatgtg aagcagagta agcaaaaaca gtctcgtcga cttttgtttc gtttgttttt
    19681 attaatatgt atcgtcgtag ggttactgat cgctttatat ccattttatg tggattcttt
    19741 aaatagtttt attgatcaaa aacgtatcga acaagtacaa aaacggaccg cagctgaaat
    19801 tgaagtgcag cgaaagaaaa tggaggagaa aaaccaacga ttaactgacc aaggattcaa
    19861 tcctggtact gatcctttca atgaaaagaa cagaaatgaa agtacaacat ccagccaatt
    19921 agaggaatgg ctgatcggaa gtatcaatat acctaagatc caattaaata tttcacttta
    19981 tgatcggctc aatggtagga tccttgaaaa tggagcaggc gtcttgcaag gaacctcttt
    20041 cccgcttggt ggaaattcga cgcattcagt gatctctgct cacagcggtt tacctaatcg
    20101 acggttattt accgaattag atcgtttaga aaacggagac acgtttattt tgactgtttt
    20161 gggggagaaa ttggcttatc aagttaaaaa tatacaagtg gtactgccag atgacacgtc
    20221 tgttctaacg atttcagaag gagaagactt agttaccttg cttacatgta cgccgtatat
    20281 gatcaacacg catcgtttgc tagtgacagg acatcggatt ccttataacg aatcggtaaa
    20341 aaaagaagaa ggaaaaggaa atcaggaaag aaccatgcgg caatggttta ttcttgcagg
    20401 aacgataata gctgtagtca tactgttagt gtttatcgga cgactaatct atcagtatag
    20461 attaagtaaa aaagtgcttg atttttcatt tattattagt gattcgtcag ggaaaccggt
    20521 aaatggtagt tcattcattt tgaaacataa gaaaaaaaca cttactagaa atggtgtgcc
    20581 gttttcagta caaagcgacc atcatgggaa agtcaagctg gatcaattgc ctggtggaac
    20641 ttatcgaatc gtttcagctg atgatcctaa agttgctgtt tcttttggta ttagaaaagt
    20701 aaaacaagag aagatatact tttttgaggg gcgaaaactt gtaaaagagc ttcaaaaaaa
    20761 tggtttttat tttaaaatca atgaataagt aaggagagaa aaaatgatcc gcacaacaac
    20821 aaaattgtct gccaaagaac tggaatctgt tctatcgatt tggctgcatg ctaacttgga
    20881 ttctcatgaa ttcattccat caacttattg ggagaatcaa gtagaatctg ttaggaaagc
    20941 tttaccagat gcagtgattt acttagcaga aatagatcaa gaaattattg gttttgctgg
    21001 ggtgattgat cagtttatcg caggaatctt tgtgaaaagg gaataccggc aacaaagaat
    21061 cggtacgcaa ctaatgaaaa aaataaaaga aaccaataaa acactgactt tgacagtcta
    21121 tcaaaaaaat caatcagcta ttcatttcta tctttcacaa ggttttacaa ttcaaacaca
    21181 gcaaaaagat gaagcagtaa atgaggagga acttgtgatg aaatggaatc aaaacgctga
    21241 tttttgatga acagaataac gaaataatgc tcccagccta attttttgct gggagcatta
    21301 tttcattttg aagtcactaa tttaactgtg tgaatatgca gttgttacaa tctttgataa
    21361 acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat atgaggaact
    21421 atttttccat ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttgata
    21481 aacggtgttc aaacggctgc ttctcggtaa taagcccact agcctagaaa atatgaaaag
    21541 ctattttcta actagcgttc ttattactca aagcataacg ctgatttcac aacctcttga
    21601 taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa aacatgagga
    21661 actatttttc cgtttctttc tctaatactc aggtcagaac gctgatttca caacctctct
    21721 ttcacaacct tattttactc gtttataatt ttcatcgaag tattttccgc tacggatatc
    21781 atctggcatt ccatcgtatg gttcttcgct aataacatct tcattgtcta tacctgcatc
    21841 acccatataa gtgatcgtag caaattcagg aacaagtaat tttggataag tttcatattt
    21901 ttcacgagct gcttccattt ctgcgatatg ttcgttttgg aaagtaacag tgatttctgg
    21961 atgttcttca acccatttag ggaaaaagat cattccgtga agtttctttt catttggcat
    22021 aaagtccaat gccacgtctg tccctgttgg ctctgtccaa gtgattttga atacgccgtc
    22081 cgtcaattta acgatatcag cttcttggtc ttttacccaa cggcctttga ccattccgcc
    22141 atggatacgg taatcaacag tatggtcatt tttggcatac cattcatatt cccaaccatt
    22201 gtcatacgta tagataaagt gagttccaat aaaatcttcc aattctttaa attctttcat
    22261 gtttaagcat ctccttataa attaaaacat gtaataaact acatgttttc gattactcat
    22321 ttatgataga atggatttaa agatttgtca aggagggaaa actgatggca agaaaaaata
    22381 cacttcgata tattttgtta gggttattaa gtaagaagaa gatgacagga tatgaattga
    22441 atcaatcgtt caaaaatgaa ataggagaat tttggcaagc aaaacatagt cagatttatc
    22501 ctgaacttgc aaaaatggaa gagcaaggaa tcattcagca tcaagtagag atcacaggtg
    22561 aaaaattgga aaagaaagtt tatgaattaa cagaagaagg gaaggaacaa ttggcagagt
    22621 ggattcatac acccaccaat gaactaccgg ttaatcgaga cgaatttgtt ctgaaattgt
    22681 atttcgtcaa agacgtgaat gatccggcat tagtagaaat tattcagcag cagcgtgacc
    22741 ttcatgaaga aaaacgactt catctattag agcgaaaaaa aacgatcttt cctactgaaa
    22801 aagaaaaaca tgaaaattac ggacactacc tgattttagc gcatgcgatc aatcgggaaa
    22861 cagaatatgc aaattggcta acagatgtat tagaagaaag taaccgaaaa aaataaaaaa
    22921 atatccgaac agtgatggag aattcctttc tatatgaaag aaacgcagat caccgttcgg
    22981 atattcttat gttcaaacag gccttttttc aagtatttat gtagcatcta cttattaaaa
    23041 agcttggtac ttaggagatt agtggcgttt agagttcttt taacaactgt tcgtagtcag
    23101 ctttttcatc gacaggaagg agcttgtaag ttaattcgtc tgtctcgttg tcatgaatga
    23161 tgtacatctt agcattctct tctttgaagt taccgatttg taaaatcgta tagcgttcgt
    23221 cgatcaattc gatatctttg attgcttttg aaaaaacttt acggacatgg tgcattttct
    23281 tttggcggat ttctgccttt tctgcagctt ttgacaaagt aaatccgtca tctaaatatt
    23341 ttttgatcag ttctactttt atgactgtag gtaaacgata ttttctagga caattcgcat
    23401 cagcttcgat cgaacgaatg aatccctttt gttcccaata ccttaattga cgtggagaca
    23461 cacctacgat atcacttaat tcactgatgc caacaaccaa ttgatcattt tccaacaaac
    23521 gtttaaggga agccccaacc atttttgtca ctccttttgt cctcttgctt cccttaatta
    23581 tatccgtatt aacttatgtg tcaagtaggt tgacaaatag cgaatttagg actaaagtat
    23641 tctagtagtt tcagaaaaaa ttcagaaaga aggagaaata tgagtaacaa ccaacccgta
    23701 gatatttacg gaaaaccata taatcggaca cttttagttg ttgtcttatt aattggtaca
    23761 ttttgtaccg ttttgaatca gaccttattg acgacagctt ttcctacatt gatgaaagct
    23821 ttcgatattt cagcgtctga tgtgcaatgg ttaacgactg gtttcttgct agtcaacggg
    23881 atcatgatcc caataacagc atggctgatc aataaattca gttcaagaaa tttatatctt
    23941 tctgccatga cgatcttttt gatcggaacg attacttgct ttacagcacc taattttagt
    24001 acattactaa tcggtcgttt gatccaagct tgcggtgtgg gagtatcgat gccattattg
    24061 caaaatatca tgctatctat tttcccgcca gaaaaaagag gatcagcaat ggggatgtca
    24121 gggatcgtta ttggacttgc tcctgcttta ggccctacat tatctggtta tatcattgat
    24181 aactaccatt ggcgtgactt gtttggtatg gtactgccaa tcgttgttct tgtcttggtt
    24241 ttagcattct tcctaatgaa aagtgttatt ccattgtcaa atcctagcat tgatatttta
    24301 tccgctattt tatcaacgat tggattcggt agtttacttt acggattttc tagtgtagga
    24361 gatgacggct ggggaagctt gaaagtaatt ggtttcttag ttgttggtgt gattttcatc
    24421 gccttgttct catggcgtca attacatttg gaacatccat tcttggaact tcgtgtattc
    24481 aaatcatcta cattcacgat tgcagctgtt ttagctggtg taacaaatat ggcgatggta
    24541 ggggcagaaa tggtcgtacc attgtatatt caaaatattc gtggagaatc agcattccat
    24601 tctggcttga tgttgttgcc gggtgcattg atcatggggg ctatgatgcc tgtgacgggt
    24661 gcgattttcg ataaatacgg cgcgaaacgt ttagccatca cagggatgtt cattttgaca
    24721 gcggcaactg caccatttgc tttcttgaca aaaacgactc cagttttata tatcgtgatc
    24781 ctttatgcga tccgtatgtt tgggatctcc atggtcatga tgccggtaac gacttctggt
    24841 atgaatgcat tgccaaataa cttgatcagt catgggacag cggtaaataa tacattccgt
    24901 caaatcgcaa gctcgattgg tacagctatt ttgatcagtg tgttgacgaa tgtgacaaaa
    24961 gcaaatatcc caagcaaatc tgtattggat aatacgccat tagcttataa agatcaggca
    25021 atcaatgcca cactgtcagg ttaccatgcc gcgtttatcg tagcgatcat ctttgggatt
    25081 cttggcttta ttattgcctt tttattgaag aaaaaagaac ctgttctaat gaaagcgggg
    25141 gataatgcat gattattgga gttttgattg tcagcttgct tgcttttgct ttaacgaacg
    25201 tgttcgctaa aaaaacatgg caaacctttt tatctttgat tttcggacta atcttcgtcg
    25261 cttcactaag tttgatcgtc gctaatttgt ccaatcactt tggaatggaa aaagtaacgg
    25321 aaacaaaaac agaaaaaatt gtttccagtg ctgattccca aggagcagat atgcttttgt
    25381 ataaagcttt agggaatgga aaagaaaaag tgtatcttta ccgaacaaat gaaaaacaag
    25441 aaaaaccaaa agcaactggt acggataacg aaacaaataa agtagaaaag acagacggcg
    25501 atgctgaaaa agtgacgaaa actacttatt gggaatacaa aaacgacatg tataaattct
    25561 ggttcaatat tgctgataac aatcacgaat atgacagccg agtgaataca ttcaaaatcc
    25621 cagagacatg ggtagaatta agtacggacc aagcagccaa gttagctgaa ttagtgaaga
    25681 aacagcaaag tacgatggaa agtgaagcaa aagcttatgt tcaagacggt atggtaaaag
    25741 caatgactga aaatccaaaa atgagcaaag cagagcaaga acaacgtacg aaagccttag
    25801 ctgctgaatt ccaacaacaa gcattcgcaa aattagtgaa agaagcaaaa ggcgaataat
    25861 attttgaaca ccatgtagga ggttgtgaaa aagctgctct gcatcaagca gtaagaacaa
    25921 tcagataaaa atagcactcc atatttttca tctgattggg cttagtgccg tagatgcaag
    25981 cttttgaaca ccgtttaagg aagaggttgt gagcctgccg tttaactctg agtcacaagg
    26041 agcaatttca aaaaacagct tctcatgttt gttgactatt gtgacttgtg accgaggagt
    26101 tggcaggaga acaccgttta ttcgaaaaaa ggaactgcga caagactttt gtcacagttc
    26161 cttctttttt ttgctatgct atactagatg taaaaaatag gagaaataaa atgagggaaa
    26221 tttattcttt tgaatcaaaa atctatgctt ccgaagtcgg caagggcggg gcatatatta
    26281 tttttccgta tgatataaga aaagaattcg gtgctggaag agttaaagtc caagcaactt
    26341 ttgacaatgt gccgtatgaa ggcagtatcg tgaatatggg cgtgaaaaat ccagatggtt
    26401 cagtctgcta catccttgga ctacgaaaag atattcgtaa aaacatcgga aaagaaatcg
    26461 gtgatattgt agcagttaca gtgaagcaaa aataaaagag gttgtgacaa aagacgtgtc
    26521 acaacctctt gataaacggt gttcaaaagc cagtttctcg gtagtaagtc aaatttatgc
    26581 aaaaatgtgg aaacccatgt ttacaaaatt tttcttacta ctcgaaacga agcggcttta
    26641 tcacaacctc ttgataaacg gcgttcaatc agctgtccct cggtattagc tcaaaaacgg
    26701 caaaatatga gaagctattt ttccattttt ttctctcata ctcaggtcat aacgctgatt
    26761 tcacaacctc ttctttcata atggtttttc accatcttta taataaactg gttctttgat
    26821 atggaaatct attttttcca accaatctaa aatcttgtaa cctaactctt ctgacatcgg
    26881 aaaagaggcg ttgatttgtg cattggagat atggtcaaca tggataccgg aagtgatcgt
    26941 acaacttccc ggaacggcag ttttgatgat cacagctatt ttttcggcta agacatcgtc
    27001 tttatggaac cgaccatcgt ggctagggaa acgtacagtt tgcatctctg tatcacgaga
    27061 aaatgtagtg actgttccga tatgtggtgt atctccgcct gttaaagtga tcaataagtc
    27121 tttaccgatt actttaacag cagcttggat cgtgaaacct agtttggtta cttcaactat
    27181 cgtttctttt tccatattaa tcaccctgcc aacgttttga atgctcattt acaatttcaa
    27241 attgatccaa aagtttcatt gtatggtgat taacaagatc ttggatcgtt tgaggctgat
    27301 tataaaaagc aggaattggt ggtatgattt gcacacctag tcgtgctaat ttagtcaagt
    27361 tttccaagtg aatggcattt aatggcgttt cacgaggaac aatgactaac tttcgttgct
    27421 ccttcattgt tacatcagca gcacgtgcaa tcaggttgtc gcctaaacca acagagatac
    27481 ctgcgactgt tttcatactt gccggtacga tgatcattcc atcggtcaaa aatgatccgc
    27541 tggcgacagc agcacctaaa tctttgtttg aataatggta atcaaacagg ctttcgaatt
    27601 cagttagtga taagtcggat tccagcttta agttctgttt tgcccatgga ctgatgattc
    27661 cgtgtgtttc tatcatcgga tattctttta gtttcttcac taggttgatg gcataaatgg
    27721 tgcctgaagc gccacttaca ccaatcacga tttttttctt ccgttgtgcc aattgttcca
    27781 ttttccgcac tcctttttat gatgtcaaaa cagttgcaga tcattcatta tttcaaatat
    27841 ttttcccaat cttctacttc ttggaatttt gcacgtacaa aatgttcttt catatcgaaa
    27901 ggaactgtac cgtcaaagat agtttttgca cttatcccac ggaaacgaat agagccagga
    27961 ttatattctg gtaattctga tgggtccaat ggatggttcc tcattcctgg gatcacgatg
    28021 atatctttat ctccttggaa acgagtgttg aaagtccaaa ctacatcgtt catgtcgaaa
    28081 atatcaacat cttcatcgac taaaatgact gtcttcaatt ctttgaaggc tgataaagca
    28141 agaatagctg cttgacgctg aataccttca tccgcttcgt tttctttacg gatttgcatg
    28201 atggtcatca atttaccacc acctgcagga ggattgtaga catttactac ttttcctgga
    28261 atagctttat cgaccaaggc aagaatactt gcttctgtag gaataccggc catagatacg
    28321 tgttcttcac tgggaccaat cgttgtttgc atgattggat tgtcttttcg gtgagtaact
    28381 gctttgactt tcactacttg tacagctgga ttagcatccc cagtatagcc aggaaattct
    28441 ggcattgctt ttcctgagtt cgtgttgata tcttcttgca ttgtttcatt tggcatgatt
    28501 tctgcttcaa tgatgaattc agcacgagca atacccactt catcgactgc tataccatca
    28561 acgatttgaa taggttcgtt acgcaaagct cctgcacacc atagttcatt gtagccaagt
    28621 ggagtggtag gtggttcgaa agtcgtgcca atggcaattg ctggatctaa accaatattg
    28681 atagtgatag gcattggttt attcaaggct tcgaattgtt tttggaagtg accaatgtgg
    28741 cgtccgccag gcatgatgta cataccgatc gtatctttat cttctagaac catacgatgg
    28801 atcgttacat cgctcattgt tttttctgga tctgatccta agactacacc cattgtgatg
    28861 tatggaccag catcttcagg agtatttgta ggtgcaggca aaatgttgcg aatatcgaag
    28921 tctgcatcgg tagctagatg aacgacttct tgtgctggag cgtctgcttt gtctactttc
    28981 acaggtttga ttggattttc tacagcgtct ttcaagaaac gaccaagtgt tcgataatca
    29041 tggtgcaaaa tttcacctac acgtttacga ctagccattg ttccgatgtt cacacgtgtg
    29101 ccggggaaac ctttgatatt gttgaaagtc atagctggtc cttcttgagt cggacgtttt
    29161 actgtaccgc cagcaccgat ataacggtaa acaccagata attctgcttc aggatcgatt
    29221 tcaatatctg tttcgtgata ttgacctgga agtgttttta gttcttcgat gacttttctt
    29281 aaatcatacg gttgatctgt catgagagat tcctccttag ttgtctttac ctatctatag
    29341 taaaaggtga aaaagaggaa aacaattcaa cttgttcggt aaagtattct acaatggaat
    29401 ggatgcgttt ttttgataat agaataaact atgatatact tatcactaaa agaaagcgca
    29461 tacttagtgt tggaggaagg aaagtgaaaa aaatggagga gtcatttcct aaagcagtaa
    29521 aagtggaaaa tatagcgaat attttgaaag ttacatttga aaacggagaa gtaaaatacg
    29581 tgaaatctca ttggacagaa gagataaccg atgccttgca atttggaaaa aaaggaagag
    29641 gaaaaagaaa aaatcttctc gcactttctc gtaatatgtg gattgggact gaagtaacga
    29701 ttgaagcaga tggaacagta ttcataaatg gaaaagacag gtatacgcca gaagagttgt
    29761 ggtacaaagg aaagaaaagt ataccagaat tataaaaaat aattaaaaaa aaacagcgcg
    29821 aaacaaggaa catattactt cctcgtttct tgctgttttc tactatttca gcgctcttat
    29881 gactcaaatc taaatgtggt tgtcacaacc ttattctgca cgcaaagcga tagcggcgtc
    29941 ttttttcgct gccattcgtg caggaagatg accgccaagc atcgtcaata tagtagagac
    30001 gatcactaga ataatcgcat ggacaggatt tagttgagca acatttttta gatccgtcat
    30061 gttgtataag atgctattga tagggaacgt agctagccat gcaataaaca cacctaatat
    30121 cccagaagaa atacctaaaa tacacgtttc tgcgtcaaat acgcgagtga tgtctttttt
    30181 acgtgcacct aaagctttta gtacaccgat ttcttttgtc cgttcaatca cagatgtata
    30241 agtaatgata ctgatcatga tcatacttgt tactaaagag atccctgcaa aagcaataag
    30301 gacataagtg atagcatcca tcaatccgcc agtcagttca gtcatcgttc cagctaaatc
    30361 agtataaatg attttatctt catctgattt gcctttattg taatcatcta gataatccaa
    30421 gatcttttct ttgtcttcaa aattgttcgg ataaatcatg atacttgaag gcaagctgtc
    30481 tccacctaaa taactaagca atgtttgttt ggcattttcg tcaacttttt cagtagttaa
    30541 gacatttaca ccactgtctc tttgagcttt gacgatttct gattctttgt tttcgttgac
    30601 gatttgagtc gttaattgat cgctataagc gattcctgga gataagagat tcattgtaga
    30661 agaagatttc acgcgtagga tccccgagat cttcaattca tcagaagcat tctgatacat
    30721 cgcatcataa tctgtattag ggataaaatt cccagttgga agttttgtat agtaagtatt
    30781 gttgtttact aatttgaatg ttgtaccaac gatatcatca aaatcaagtt tttcgtcttc
    30841 tttcacatca aatccaagat tcttcaatgc gttgatatta gtattattat tgccgtctac
    30901 gatcagcacg acatctgttg ctgaggctgg ataacttccg gcaagtaaag aataattgtc
    30961 ttttaaaaag ttttctttgc tagtgtcaag ctgtgtaggg aaacttgaca ctcccacacc
    31021 tgtcattgct gacatcgtac tagataaaga aagagattcc gtatcaggat tttgattcga
    31081 gaagctgaca ggctgtactt tgccatttac atcacgtaaa agattgattc ctgtagtacg
    31141 ggtaaatccg atattattac ttaagttagg atcgatgtct gttacgtaat cgacgtatgt
    31201 ttgatcaatc ttattcgtgt gctgtgcttt atcttcttcg ctgacttttg cagtaatcgt
    31261 tttgctgtct ggataatccg ctttgcttgc acccaatccg gcatcgtcac gagtctggtt
    31321 agtcgttacc ttagaaatag taatcggaaa tttcgccatc gtttcagctt gcgtgttatc
    31381 gatttgtttt tggaatccag aagaaaggga cagaacgatc gcgattccga tgatcccaat
    31441 actggaagca aaggaggtca agaaagtacg tccttttttt gttcggatat tgttgaagga
    31501 aagctttaac gctgtccaaa aactcatctt tgttctgcgt aagttaaatt gatcatcttt
    31561 tggtcgttcg atgtgaggat gggaatctgt taagatctta ccatcagaaa attcaatgat
    31621 tcgatctgcg tattgatggg ccagttcagg attatgtgtg accatgatca ctaatttttc
    31681 tttcgataac tcttggatca attccatgat ctggatactt gtttctgtat ctaacgctcc
    31741 ggttggttca tcgcacaaaa ggatatctgg gtcattggct agtgctcgag cgatggccac
    31801 tcgctgcatt tgtccaccag ataactgatt tggcttttta tgcatatgat ttgtcaagcc
    31861 gactcggtgc aatgcatctt ctgcccgttt tcgtttttcg tctttagaaa caccacttaa
    31921 tgtcatccca agttcaacgt tttcaataat gcctagatga ccaatcaagt tgtaactttg
    31981 aaaaatgaaa ccgatagagt tgtttcggta ggcatcccag tcactgtcct taaagtcttt
    32041 agtagacttt ccgttgatta ccatatcacc ggaatcataa ttatctagtc caccaatcac
    32101 attcagcata gtcgttttac cagaaccact aggacctaaa atcgctacga attctttttg
    32161 tctgaaagcg acagacacac catccaatgc tttggtggtg ggttcgccga ctttataata
    32221 tttttttata tcttttaatt gaagcataat cacccttctc ccgtattgaa atattcggta
    32281 tactaataag tatatcagaa caatgatcgt agtatacgaa ataaatgtta actgaagatg
    32341 aactacaaga aaaaatctag aaatttttca aaaaacagag ggagatcaat gaatcgtatt
    32401 ttagtagtag aagatgatcc gcaattacag ttgctttacc gttctgtgtt ggaacgagcg
    32461 ggcttttcag tcatcaccgc attgaatgga gaggatgcgc tgaaaaaact agaagcgaca
    32521 cgtatagaat taatcattac agatatcatg atgccgaaaa tggatggcta tgacttgcta
    32581 gactcattac gtagcaccag atttgacaca cctgttctaa tgattacggc aaaggccgat
    32641 tttgaggata agaaacgcgg gttcaaatta ggagcagatg attatatgac aaaacccatc
    32701 gatgtcaatg agatgatcct acgtgtggaa gcattgcttc gacgttcaaa gatcaattac
    32761 agcagtagct tgataatcgg acaaacaaaa ttagagctag atgcttatac agtgttacaa
    32821 gatcagcatt cgttgattct gcctcaaaaa gaatttctgc ttctgtataa attactgtct
    32881 tatcccaaca agatcttcac tcgccaacag ttgatggatg atatttgggg attagatacg
    32941 aatacagaag aacgaacgat cgatgtacat atcaaacgtt taaggacccg ttttgaaaag
    33001 acggaagatt ttcaaatcat cacagtacgg ggactaggct ataaggcggt tatttaaaga
    33061 tgaaaaaaaa gctattttca caattatgga tctattttgc tagtattgtc ttcatttcga
    33121 tcttagtgac actgctgtgt tttctcggtt ttattttctt attgtctcga cattctatct
    33181 cgcctgctga atcggctgga aaaccaacgt tatacccgtt attcgtcttt gcggggttca
    33241 gtatggtcgt gggtacagga atatcgattt ttgttgggcg gcgtatcctg catccaatca
    33301 gtgcgctagg gacaaatatg agtctggtag caacgggaga tttttcgatt cgaatggatg
    33361 aacagcagaa agtagcagaa gtacagcaac tgtataaaga tttcaacgtt atggtccaag
    33421 aactcaatag tatcgagaca ctgaggaatg actttgtgtc cagtgtatcc catgaattca
    33481 aaacaccact agcaacaatc caaggatatg ttcaattgtt acaagcccca aatctctctg
    33541 acgaagaacg ccaaatattt ttatatcgga ttatcgaaag tatcacgcag ctttcacaat
    33601 taacagagaa catcttaaaa ttgaacaaac tggaaaacca gcgtatccaa ctggaaaaga
    33661 aagagtatcg attagatgaa caaatcagag aagtaatcgt tttttttcag cctaaatggg
    33721 agaaagaaca gctagaattg gatatcgaac ttgctgcagt aaattacaca ggcaatgaag
    33781 agtttttgta ccaagtgtgg ctgaatatca tggataacgc cataaagtac aatcaaatca
    33841 atggacagat tcatatcaaa ctatttgaga cagccacaga gattgtactg gaagtgaccg
    33901 attcaggtgt aggaatgaat gaagagacca gagatcgaat gtttgaaaaa ttttatcaag
    33961 gagatacaag tcgtcaaatt tctgggaatg ggctaggact ttcactggtt aaaaaaattc
    34021 tagaactgca tgatggacgt attgattata gcagtatcga aggagtagga acgacagcaa
    34081 tgatacggtt aagtaaacaa taggaaaaag aataaaaccc ctaaaaattc tattgaaggt
    34141 ttttaggggt ttttagtcgt ttttttgtga ttagggaaat gttcttacgg ttcttttggc
    34201 ataaaaatcg ctgctttctt attgaactac gagaaatgtt agaggtgtca ttgaaacaag
    34261 gacatgatgc aagaaagaga gcgaaagtca tgcgtcgctc tctttttttg ggtggataga
    34321 tggacagaaa atgtagggct tagttttctt cttgatttaa atactcccaa aatgtatctg
    34381 aagccaaaga atgctcaccg actttacgag taaatgcaag ataactctct gttgttggag
    34441 aaatagggag aatcacagta ttttctttta atagattctt gactgttttt tccataacaa
    34501 tagcaatccc catgtcattt gcaatcatat tgatgatcaa gtcgattctt gctcctttat
    34561 aagagatttt tggttcaaat ccagcttcgc gacacagatc tagtacaggt tgatacaagt
    34621 tcgtagtggg gccaaggagc aaaaagttct cctttttcag ttcgcttaga tccaatttct
    34681 ttttactagc taaaggatga tttttaggta gaactgctac aaaatcgtct gtttcagtag
    34741 gcaaccactc aatcgaactt ggtggggcct ggaaacttct agcaaagtgg atcgtgtcac
    34801 tatgttcatt ggcgtcattg taagttaatt cgacactttc cacttctttc aactggactg
    34861 taaattcagg atggcgtttc aaaaactcgg tgattttgga aaaaccttta taattgatca
    34921 ttgtcggtat cgttaagata tttaatgaaa gattgttgtc tttcgtatag gtttcaagca
    34981 cttcttgcat agcatggtat tgttctacga cattttttac atatgggagc aataagcttc
    35041 cagcttcggt caattcgatt tttctatggg cgcgttcgaa taatttgacg tctaattctg
    35101 tttctaacga aagaatctgt ttagaaatat ttccttgggt ggtgaattgt cgatcagcag
    35161 ttttggtgaa attcattgtt tcggctaaat caagaaaaac ttcgaattta tggatatcca
    35221 tcatattcct cctacttatt ccaataaaga atactttaac acaaaaaatg aattggtgta
    35281 atcttctctg tctgttacaa tctttcttgc agcgaaagca ataattacag aaatttttct
    35341 gatttttttc gcttcgtaga aaaagagagg agttttttga tgccaactgg agttttgatc
    35401 aatgtaggtt ccgttttgtt tggcggacta attggcggat tagtaggaaa taaattaagt
    35461 gaacatttta aagcacagct gacgatggtt tttggtgtgt gttcgatggg gatgggaatc
    35521 tattccattg cgccgatgaa aaatatgccg gctgttattt ttgctttagt gataggaaca
    35581 gcgattggtt tgattgttca tttaggtaat gggataaata agggggctgc gctgatgcaa
    35641 gtacctattt ctaaaatctt ccctagtgaa aagcttggta tgacccacga tgaatttatc
    35701 agcacattag tgacggtcat tgttctattt tgtgcaagtg gtacggggat ctatggaagt
    35761 ttagattctg ggatgacagg agacagtacg attttgattt ctaaatctgt ccttgatttt
    35821 ttcacagcag ctatttttgc gtgtaattta gggtatgtcg tttctgtgat agcaattccc
    35881 caattcatta ttttttatat tttgttctta ttggcaaaat ttatttatcc attgactaca
    35941 ccagacatga tcttagattt taaagcttgc ggcggattct tgatggtcgc taccggtttt
    36001 agaatgatca acgtgaaaat gttcccagta gctgacatga tacctgcaat gatcgttatc
    36061 atgccactga gctggatgtg gacaaattgg attatgccac tgttataata aaaatcaaag
    36121 agaagccatt ttttgctgac taaaaaggat caaatctttt agtcagtttt tttgtatttg
    36181 acaaactgat tttgaaacta tattatatgt tgtataatat agattgaaat aaaatattac
    36241 acttgagaat agaaggtgac gtaatgcaca tccagattcc aactgttctt cttgatggta
    36301 ctgtgctagc agcattaagc aaggaagata tgtatgggta tgccttaaca aaaatcgtac
    36361 aagaaagtct ctctgtcagt gaatctacac tatatccggt tttgagaaga ttgaaaaaag
    36421 aaggatatct tgagacctat gatgaacctt ttgaaggcag aaaccgtcga tattactcgt
    36481 tgacaccaat gggaagtcgt catttagaag aaattattga tgaatggatt gtttttcgag
    36541 attctttaga ccggttattg gaggagagca caaatgaatg aatatttgaa tgaagttgtg
    36601 gatcatcttc aagaaattcc tgaagacgat cgttttgatt tgatccagta ttatgaggaa
    36661 tattttttag attctggaaa aacattagat cagatcattg atgaatatgg tacaccaaag
    36721 cagtttgcct taaaattaaa aatcagttac ttttcagaag aagaacaagc agaaaacgag
    36781 ttagtgaaaa atccaaaaag acaaattcgc ctgatttggt taatcattat cggtttattt
    36841 gcttctcctg tcctcattcc actagcactg gggtttatat tggttattgg gggactattg
    36901 gttgctttac tagcgctcat ttttagtatc tatgttttat gtatcagtat cttaggtgtt
    36961 ggcctattca tgatcatctc aggaatcagt gtgtttggac aatcgattct gagtggatta
    37021 ttctttatcg gtgtcggtct gttcgccaca ggaagcgcag tatttatttc gccactgttg
    37081 ctgaaaggaa caaaagggct tttttatgtc atgatgaact ttgttaaatg gatgggacga
    37141 cgttttatta caaaaagaga tctgcattca gcgggcgttt gaaggaggac acataaattg
    37201 agattgaaac attttttgat taccggtatt ggtctgatgc ttactggtgg cctcctagca
    37261 ggtgcaactt atacattagg cgctcaaaag agtctgactt gggataatgg tcctcggttg
    37321 atcgatatgg ttactgagac aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca
    37381 gaaaatcaaa cagtttcggt catacgcgga ttggattttg agataaaaac gacttatgat
    37441 aagacagaaa agccagaaat aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa
    37501 aaatcaacag gtgcaatcat tagtttagaa gacacaagtg cacaaatcac tattgttgtc
    37561 cctgcagatc atgaattatc tgaattgaca atcgaaggga ataatagtgc aattgatatc
    37621 aacgaagtat caatcaaaaa agtgaatact aagacacatg atggctgggt atcgttcaat
    37681 gaatttacag gaggaacaat aactgcgaaa gatgaattag gctcacttcg attagatgag
    37741 gccaatgtgg ataacttgat cgtcacaggt gaaaacagca acttccatct agaagacagt
    37801 ttgatcagca aagaaggaaa agtcatccta aacggtggca atttatcttt gcatgagatg
    37861 gaaaacagtg gctatactat ccaaacgtct ggtgaatctt atattgaaaa agactaccag
    37921 ccaataacaa atactttgtt agaacaagga cagaaaaagc taaagatcac tgctaaagac
    37981 acatcgatca atattacgac ggatcaagag gacaatgaaa cagattgatc ctgtactcaa
    38041 ctggatattt taagaaaaaa cagcaaagat ccttccaatc tttgctgttt ttcatcgctt
    38101 aacgcacata actatagtaa aataagaaag aaaatatcta aaaagaaaac agggtgaaag
    38161 agtatatgaa ggtagaagaa ggtcaaagag aaaagttaaa aacagaaatg gaaagattac
    38221 atacttatat cactcaacta agccaaacct tctatgatcc agacaaagaa aaagtgatgg
    38281 ttaattaccc gaacaattca gaaggtcgcc aattagagca agtgtaccat gaagttttca
    38341 aacatttatt gacggtgaag aaagagctgg attattattc tttaccgatt atcgatacag
    38401 gaatattgaa gtatgatggg aagaaggaac gatttatctt taaatctgtt cgggaagatt
    38461 tgcctctttc agcagggatg gatctagagg tcttggtaga agactatttt acagaagaaa
    38521 agcactgggt tcgaacaaaa ttggattatt taccgcaggc agccggaggg acacaggcaa
    38581 gcggttggta catcactgaa gacaaagagt tagaactaga aggtgtaatg gcgcgtattc
    38641 gaaaaaaaaa ttaaaaaagt aacattagat atttattgaa ggataaagga gcgcaggaag
    38701 tagcatgaga gaaaaaagag cattttatat caatggttat gcagggattg tcggattggt
    38761 cgtattagct ttagttggat tgtttttctt ctatttaggt atgtggcaag cgaaagtttg
    38821 ggcacttttt ttaagtattt ttctttggtt gatcacgtta ttgttgttaa gttcggcaac
    38881 tgtagtcagt cctaatcaag caaaagtaat tttgtttttt ggacaatatt taggtacgat
    38941 tcgagaaaat ggctttttcc ttacgatacc tttggcacaa aaaatgactg tttcgttgaa
    39001 agttcggaat ttcaatagtt ctgttttaaa agtcaatgat ttggatggaa accccatcga
    39061 aatctcagca gtagtagtct ttaaagtcgt tgatacagca aaggcattat tcgatgtagc
    39121 gtattatcaa gattttgtag aaatccaaag tgagacagcc attcggcata tcgctagcca
    39181 atacccgtat gatacattca atgagaatga tttgacgctt cgaggaaaca cgacggcagt
    39241 gtctgacgag ttgcaaaaag agctgcaaga gcgtttagca gtagcaggtg tcgaagtaat
    39301 cgaaacacgc ttgaaccatt tagcctatgc aacagaaatc gctagtgcca tgctccaacg
    39361 tcaacaagca aaagccattt tgtctgctcg acaaaccatt gttgaaggag ctgtcacaat
    39421 cactcaaatg gccttagagc aaatcgaaga tggacaagaa atcaatttca ccgatgatcg
    39481 gaaagtccaa ttgatcaata atttacttgt ctcaatcatt acagacaaag gaacgcaacc
    39541 tgttatcaat acaggcgaca tgaaagaccg taagtgacat actttttgta agataggaag
    39601 aaggagttta ggacggatta ttgtcttgaa ctcttttttt agttgtttta aagaaaatga
    39661 caaaattgaa agaagaatga tagttgattc gaaggcagaa ttgttcgctt ttttctataa
    39721 tcagtatata aagtaagaga gaaggggaaa aagtgaaaaa acttctgatc ggtctacttg
    39781 ttttcatcgt tgtggtactt gcaggggtga aaatcgcaga ttacgtagtt atgggtggag
    39841 aagaatacta tgtacaaatt acgacagatg gtacaaaaac agtttcaaaa tcagactctg
    39901 gacaagaaac agtcaactat agatatatcc tgcctggcta tacgaaagag ggtaaggaaa
    39961 aagaactgga cttcaaggga caattgactc gaccgcttcg taaaggagca tatttaaaaa
    40021 tcacctggaa caaaaataaa ggtgtcactt cgtatgaaga agtcgaaaaa aaagacgtgc
    40081 cgaaaacagc actggaaaaa ctaattaaag ggtagggaaa gaaatgaaca aaatagtcga
    40141 agtacagaat gtaacaaaag tatacggaaa aaacaatgag aaaaagacgc aagcattaag
    40201 cggcatcagc ttcgatgtag aaaaaggcga atttattggg atcatgggcg cttctggttc
    40261 tggtaagtcg acacttttga atattttatc cacattagat aaaccaacag atgggcatat
    40321 ccgaatcaat caaaaagatg tgaccacatt aaaaggaaat caactagcag attttcgtgc
    40381 gaatgagatt ggctttattt tccaagattt caatttatta gaaagcttga cagcacaaga
    40441 aaatatcgct gttcctcttt ctttacaagg cgtcagacca aaagaaatca agcaacgtgt
    40501 ccaaaaaatt gcagaacgtt tatccatctc tcatattttg gaaagctatc cttctgagat
    40561 ttccggtgga caaaaacaac gggtcgccgc cgcacgtgca ttgatcacac aaccaacgat
    40621 tctattaggg gatgagccta caggtgcgct ggattccaaa agtgcccggg atcttttaga
    40681 tacgatggac gaattgaata caaaagatca tgtatcgatt cttcttgtga cgcatgatcc
    40741 gttttctgca agttattgcc aacgtatttt attcatcaag gatggcggta tccatcaaga
    40801 agtgaaacgt ggagatcaaa gccgtgattc cttctatcga gaaatactat ctattcttgg
    40861 aaacttggaa cagtaggggg agattgacta tgctttggaa attatcactt actgggatca
    40921 agagccgttt aagagactac ttggtgttat tctcaggatt agttatggca tctgctattt
    40981 tttacatgtt tgaatcaatg gcaagcaacg aagctttttt gaaaagtaat acgatcgttg
    41041 cggctacagt tatcattttc cgattcggtt ctgttttact agggatcatc acattcgttt
    41101 atattttata tgcaaattcc tttttgatga cgatgcggca aaaagactat gcaatgttta
    41161 tgatgctggg agcaaaaggc agaaaaatcg cacagatgat tttttctgaa acattcttag
    41221 tagggatcgc agctacttta gtgggttcag ctgtcggtgt aggattgaca agtatcgtcc
    41281 accgattatt agtggatcag ttagatctca atatttcgca ttttacacct tttagtattc
    41341 acgggttgtt gatcactgtt ttgttttttg ctgttttgtt tttattagct gctgttgtca
    41401 atgctttttc gattgtcaaa aaaccgattt tagcattgat tcgtgcggat cagacaccaa
    41461 caagaatcaa gcagcataaa ttcttgtttt tccttgaagt agtactggga attgcttttc
    41521 taggctgcgg ttactatatg atgaaacacg tggggaccta tcaagtcttt ggtatagggg
    41581 tagccttagt tacgatcgta ttaggaactt atttcatttt ccattctgtg atcattttct
    41641 ttttatcatt actaaagaaa tcagaacaga tttcattgaa aaaattgaac aatttcacat
    41701 tgtcacagtt aaactttcgt attcgtgaat atacacaaat gctctcaatg gtcgctatgc
    41761 tgtttgcctt ggcactgggc gctttgacgg taggattagg ttttaaaaat gaaattgcag
    41821 acttgacaga aaacaattct ggatatgatc tagtgatgaa taatcctcgg gcagaagatc
    41881 agcaaaaaat caaagaactt tcaccaacac tttctgcttc ttatactcaa aaagaagatg
    41941 cgggaacgat ttactataat gcaagtgaat ttgcggctca accattgatc atgataaaac
    42001 atgaagatgg aacaccgccg aagatcacga aagaaaaagt tcctgctaaa aaaatgaatg
    42061 aactgactgt acaagaggaa cttcgaagct atgaattgcc ggaacagcaa gaaaaagaaa
    42121 tcaaggtagt cagtcaatca gaatttgatc aactgaactt gccacaatcc actttacagc
    42181 tagttcaggt aaaagacttc caagcaaacc tgaaaccaat cgaaacactt gttgctcaaa
    42241 ataaaatgaa caatccatct ttgcaaaaag tagagtacag caaccagaaa tatgaaatgt
    42301 atgaagtgta taatggtcta ttctctggtt ttgaatttat gggattcttc ctaggtttag
    42361 ctttcttgac catgcttgct agctgtttga tgttcaagat cttgtcagga agcaagagtg
    42421 acattgctcg ttatgaaatg cttgaaaaga taggaacacg acgcgggttg cttaaacaat
    42481 cgatccgtag agaagtaggc gtgttattct tagcaccagg aatcttaggt gtagtccatg
    42541 tcttattcgg attacaaatg tttggtctat tcatgcaaaa tccatacaaa gatatttggg
    42601 ttccgtttac gatattcttc gtattgtatt tcatctacta tgtactgacg acatggttat
    42661 atactggaat cgtattgaaa aagagattgt gaaaaagctg tcctgcatca agtaataaga
    42721 acagccaagc aaaaatagct cctcatattt ttcgtttggt tgggcttatt accgcagatg
    42781 caagcttttg aacaccgttt gttcggaaaa agaggttgtg acaatatttt tgtcacaacc
    42841 tttttttcac ggtcttttat tagaatatag taaatttttt ctcgttactc acggaataag
    42901 ctgttaagaa aacggtttcc atgtatactg gaatcagtgc aaatgaaaga aaagagggtg
    42961 caaggaacat ggaaatcaat gaacaaaatt ttggcgaaaa ctatcacttg attacgatca
    43021 cgaatcaaaa tgggacatct ttgtctgtca gtgatcttgg ggcacgggtt gttcattttc
    43081 aaacaataat cgataatgaa aaacgagagt tgatcttagg ttttgatgaa gcaaaagaat
    43141 atctagaaaa agacccttat atcggtgctt caatcggacg tatggctgga cggattacga
    43201 atggcacatt caaattagat gatgaaacgt tcgaagtaaa aacagacccg gacactggtc
    43261 attgtttaca tggtggtgca cctagctttg aagccaagca gtggcattat acagtcatca
    43321 atggtgaaaa cgaagcatcg gtcattttct atcagactag cccagatatg gaaaacggat
    43381 ttcctggaac tttagatgta gaagtacggt atacgctgac gaacgatgat acatggcgag
    43441 tgacggtcca agggatcagt gaccgcaaaa cagtattcaa tcctacaaat catgtctatt
    43501 tcaatttaac cggtgatcca gcacagtcaa tcgacgaaca tatactttgg ttgaatagcc
    43561 gattatttgc tacgctcaat aaagatacaa cgcctacagg agaagtagtt tcagtaagtg
    43621 gcacagcatt tgacttccaa cgtcctaaaa aattaggtga ggtctttgcc agtgattttc
    43681 gccaatcaat gatagtgggc ggcatcgatc atccattttt gttgaaaaaa caatctggtt
    43741 tactaaatgc tgcggaattg acaagcccag atgaaaaagt atcgatccaa gtaaaaacag
    43801 atgcttcagg aatcgtgatt tttacagcaa attttggaga agatggaccg gaaatgcgcg
    43861 ggaataaatt agtaaatcat ggcggtatca catttgagac tcaagaactg ccaggagcgg
    43921 agcgacttga aagttttgga gatgtcactc tggaacctaa ccaagtacgc gagtttgtaa
    43981 cggaatacaa aataaatata aagaaataga ataaattaaa aagggagtgt gacagtgtca
    44041 catccccttt ttaatttatt ctatttcttt aaaggcagat caagccagaa tgatttgtct
    44101 aaacttaaca aggttagtac aggaacagtt gcagctttaa ctgtacgagt agttgtttct
    44161 ggtatctgtc ttgccgtaaa attgttccac aaatcccaga ttgaagcgac acggaagatg
    44221 gaagaagcgt tgttgacgcg ttttatcgtt gaaaattcta atcctgtgat tggcacgcca
    44281 tctgttgtcc atttgattgg cacatcgaat cgcttagaca tattgaggat ttcagcgttt
    44341 gcttcttcag ttgttgctgc ttcttttttc ggttcatcat attttgtcag gtcgtacgct
    44401 tcgatgatcg cgttcaattt ttgtgaatat tggcgatctg tagcataacg tccttccaaa
    44461 tatttggttg cttctttgta gtcttttgtt tccgacttcc aagttggttt gtagaaatca
    44521 ggattgccag tcagtccatt tttgatcaag tcggcatagt cttccaaact ttccttgtaa
    44581 gaaggatatt tacggaattg tgcgcggatt tggtagcttt gtccggaact atcttgttca
    44641 agtgtattga aggtcacgct gcttccttta tatgatcctt tgataccaaa aaggttatga
    44701 tttggttcgc ttgataataa gctgtttcca gatccacttt caaggatagc ttgagccatc
    44761 atgactgatg cgtagattcc ttctttttgc ccgatttcct gtgcatcatc tgcaatcttt
    44821 ttcaagaact gatcagttgt ttggttttga ctgaaatgga acgagccatc taatccatct
    44881 aaaatagatg agccgcctgt cagcgaacct gaagcagcag gtaaacctgc taagtttact
    44941 cggacagcaa aatcaggcgt gaaatctgaa atagaagtta ctcgaacttt atctcctggt
    45001 tgaggcgcat ggataaattg tccgttccca atatagatac ctacatggtg agtaacaccg
    45061 gaagaaggtc cccagaagac gaggtcgcca ggttgggcct gacttactgg aatctgcgta
    45121 cctgcatatt gctgttcgcc tgtccagcca ccgatataat gaccggttgc tttcatatat
    45181 acgtaataag tcaaaccaga acagtcaaaa gtatttggac cttttgctcc ccatacatat
    45241 ggttttccaa gatgcttgaa tgcttctcga acgattgctt gttggacatt cgttgtattt
    45301 gtaggcaata cagcatcaat tggtgatttt acaacaaaat cagaagaagc agaagatgga
    45361 ttctctatac cgctttgatt tgctgtattt ggacgagatg aagcattgtt cgttccgcca
    45421 ttcgatgaac cattgtttgg atggtttggt tctctctgag tatttccatt gttattcgat
    45481 ccagatgggt ttgtttgatt cgaagaattc ccgctgttcg aattattgtt attattgttt
    45541 gaattatttg aaccattatt attgttgtta ttacccacat ctggtttgtt tggcgtttcc
    45601 ggatctggtg tactagggac ttcttcacta ggatcagttg gtttctctgt tgaagtatcc
    45661 ggtccagctt taaattctgc agtaatagat aaattgttcg tacctactgt aaaggtcttt
    45721 gcatcataat ttacttcttt ggcaccatta acagcgactg aagcaatttg ttcaccttca
    45781 gctggcgtaa tagtgtaagt taccttcgtt tccgctaata aaccttctac cgttccactt
    45841 tccgtaatat ccaaaggttg ctgttcaggg gaatccgctg tcgaagcagt cagttggatc
    45901 ttaccaatca aagaaggagc aattgtaagg gaatatgtct cggctggctt ttcattttct
    45961 gatggatcag ccggatcaat ttcagttggc tcggtcgatc ctgttgttga gttagttgtt
    46021 ccagttgggt cttctgtaaa agaatccgtt gtttcactag tagctgagct ggatgtagtc
    46081 gtatctgttg tgactgtttg atcagctatt tgattatcta cggcaaatac ggtattggtc
    46141 gacaaaacag acggagcaat cagccaagta gcagctagca aacgtatcgt ttttttattt
    46201 ttcataataa actagtcctt ttcctttcat tgattttctg tcttattcag cattcattag
    46261 acattttttg gaacagataa tcgtgtcgaa aagctttttc ttttcataaa tctgtctgtg
    46321 tattctaaat aaaacaattt gtaaataagc aacgagcatt cattcgtttt tctttgacgt
    46381 tcattttatt gcatagacca cctttgtttg gggataaaca agtggggata aaaatggtta
    46441 tgcatatgta aattatacca aagtttggaa agtatcacaa aaatttataa aaataatgat
    46501 cctaaaaaga aaaagactga aatatgtgat aaacatctca tacttcagtc tttttcatta
    46561 actattcata agacattagg tcttcatcaa acccgctaac gagagtagtg atcattgcta
    46621 cgactagact aacagccagt cctgctaagt accagaaaaa agtaccggaa atgaatgcag
    46681 gcaatgtggt cagactacca aatacaaaag cgatcgcttt cacttgggca aatcccatta
    46741 cagctccacc agcagctgca ccaatgcaag cagcaacaaa tggttttttc aatgggatcg
    46801 caattccgta aacagttggt tcggtcactc ccgcaagtaa aatcgaagca aaagcagaac
    46861 cggagatcga acgggttttt gaacgtttac ttcgcagaaa tacacctaaa gctgcaccag
    46921 ccattccaaa attggctgct cctgtcaacg gaacgattcg gtcgaaccca taagcagaga
    46981 tattactgat catgacaggg tttacagccc attggatacc aaatattacc aaaatattcc
    47041 aaccaccagc gatgattgcg ccactgatcg gtccgttaat atccatcatc caagtaattg
    47101 cggcagctaa accttcgcca aggtaaacac ccgcaggccc gaataccatt aacgtcaaag
    47161 gaaccgtgat aactaatgaa agtaaaggta caaatactaa ttgcatattt tcgcgaatat
    47221 attttttgag gaatttttca acaaaagaat aaaagaaaat acctaaaatc gccggcatga
    47281 cagtagacgt atactgcatc agtactacag gaatccccag aaaatcagta gctgctttgc
    47341 cactgtctga aagtaaagag gtcagattcg gttcaatcag tgctgcagca atgacagccg
    47401 caatatatcc attcacttta aacgttttgg cagcagaaaa agcaagaaag attggcagaa
    47461 aataaaagac ggcattcgac gcagcagaca aaatatgata agttccgctt gtttcactaa
    47521 gccagccaat cgtagtcgct aaaacaacca gaccttttaa tataccagaa ccggcaaaca
    47581 gcggaatgat cggcgtaaag atttccgata tcaaaccaaa tagtttttcg atcggattta
    47641 gcttttcttt cggagctgaa tgatcgattt gacgcgtcaa ttccttgtaa gcaggagcca
    47701 atccctgatc catgattatt tctgcatatc tatcgagtaa aatgaaatac ttaatggaag
    47761 ggatttgctg ttttaatttt tctgcattgg ctactggctg ttttttcaat ttgatacgaa
    47821 gccgagtcgc acatggaagg acagaatcaa tattgtcttt tcctcccaac tgttcaatca
    47881 gcatatgaat ctgctcggac agtaccatct gttcaggatt cattgtcgtt tccattagaa
    47941 gaccactcca ctcactaata taatattggc gtaaggtgtc atttcgcctg tacgaataaa
    48001 tgcatgacag ttattcaact cttttttcat ttcttcatgt gggataaagc taatcggtgt
    48061 atctggtaac aataatttga ttttttccaa tatttccggg ttcattgtct ttatttcttc
    48121 cgccaattca atcgattgta cctcaagttc ttctaaaacg ttttctaaaa cagataaaaa
    48181 acttggaaga ccgtttgaga cagccaaatc tattttttca gtaccaaagg ggacaggcat
    48241 gcctgcatcc ccgatactta atttatcaaa gtgccccatt tgtgcgatta ctcgtgaaat
    48301 atctgaattg attacttttc cttttttcat gtttgtttct tcctctcatt gttaatttta
    48361 gactgctgat tgttctaaag cttcgcggta agggatagaa ggttgcgcac cataagactg
    48421 cacagcaaca gaagaggcta gatttccata aaggatcgct tctttcaagt tactaaggtc
    48481 tggctgaagt gtgctgctta atgcgccaat aaatgtatca cctgcggctg ttgtgtcaac
    48541 ggcatccact ttaaaagcag ggacgatacc gtgttctttt tctgtatgat agaaagcgcc
    48601 ggcactgcct agtgtaatga ttactgttcc aatacctaat tcatggagtt tttcggctgc
    48661 tgcgaccaaa ctgttatgat ctgtcactct gattcctgta atgatttctg tttctgtttc
    48721 atttggtata atgatatctg tcttttcaag tagttcagta ggaatcgttt cacgagctgg
    48781 tgcaggattc aggatcgttg tttttcctgc gtcttttgca atagaaaaag ctaggatcgt
    48841 cgcatctaag ctactttcaa actgagcaat aataaagtca ctgtttgtaa tcagttgctt
    48901 gttttttagg acttcttgtt catggaaagc attatttgcc cctgcatgga tcaagatact
    48961 attttctcct gctgcgtcta ccataatcat tgcttgtccg gttgtggcgc cttggatttc
    49021 agctatccca gatgtatcga tgttttcttt tgtcagtaga tctaataact gttgtccttc
    49081 gctgtcagcc ccaacgcctc caataaaaga tgtccgtgcg cctgaacgtt tggctgccac
    49141 tgcttggttg gcacctttac ctccgccagc gtgaaaaatc tcatgcgcat gcatcgtttc
    49201 gcctggcttg ggcatgttgg ttaaacgaag ggtagtgtcc atattaatac ttccaattac
    49261 tgtaaccgtg ttcattttac aaacctccaa ctatctcatt tattttgatg ggaaaattat
    49321 tttttaaaac gttttagtaa aacgttttaa tagttcgata aaaatatagc acaccttgaa
    49381 agctatttca agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa tcttttttct
    49441 tcccattctt tttctgggaa ttggatccga tctaacaata acttggcaga tgtttgtccc
    49501 aattcgaaga ttggttgtgc gatcgttgtc agtttcggct tgatatactc gcacatatct
    49561 atattatcat agccaataat gctgtagtct tcagggatgc tttttccagc ttcctctaat
    49621 ccgcgatata atccaaaagc taattcatca tttaaagcaa acacaccagt tgcagcagaa
    49681 tcaagaagtt ctgctgtgat ctggtagcca ccttgttttg agaactgtgt aggtaagagg
    49741 atcagttggt cgtgagggat ttggtaaaca tcaagagcag atttaaaccc ctcaatccga
    49801 gcgtggacat tttccggtgg attttcagga tagacaaggg cgatcgtttg atgtcctaga
    49861 ctaagcaagt gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg tacggcatca
    49921 ctaaatcctt cagatttttt ctgatccaaa acaatgaagg gccggccttg ttttttcaaa
    49981 ttttcgttga ttgcatctgt agagacagca gaagtcgcga taatgaatcc atccacacct
    50041 cgacgtgtga gctcggctag atattcgatt tctttttgat gatcagaatc agcattacat
    50101 aatatcgtga cgaaattctg cttatacaag atatcctcaa ttcctcgcat cagtgtactg
    50161 aagaatggat tcgtgatatc aggaacgagt acaccaattg ttttcgtttc cttggtaatc
    50221 atttgtctag caaaatagtc aggctgatag cctaattctt ctttggcagc taaaactttc
    50281 tgaacagttt tcggactgaa tttctccgta tttccattta atatcaagga gacagttgtg
    50341 attgaaacgc cggaaagttt agcgacttct ttgattgtga tctttttttt caccatgttc
    50401 atactcctat ctaaatcctg tggttatttt cccatgaaat caaaaagaag taaatgaaaa
    50461 tcacgtgtgc cagtacttat ttgctttgtt ggatagcttc gattatttgt ttcgttgagc
    50521 gtactcgtgc agttttcgga aatatctttt ttatgaacaa ttgatgggat tctttgtcac
    50581 gatcactgca agcatcttca gcaagcacga tatggtaccc gtgttgaaag gcatctaatg
    50641 cagtagcata tacaccgttt gttgtcgcta caccgcttaa aatgatagtt tcgattccac
    50701 gccggcgaag ttgaagatca agagaagtac caaaaaaagc acttggattg tattttgtga
    50761 tagtcaacat attatctgta tctttcaaag aatccttcaa aagtaaatca gtgaattctg
    50821 gaggtacaga agcagtcatt ccacccatat cagtttgttg actcaaataa gggaaagtgg
    50881 agatgtctac gttcactaaa actttcaagg cagcggtacg tttcaatgtt cttactaaca
    50941 aatcattgtt tgtcagaata gatgctgcag tgtgtggaga gagtgtaccg ttatttacga
    51001 tctctttttg catgtcgatc gcgacaaaag cagtttttga taaatcaaaa atcatatttt
    51061 cctttaaatc attcatgatg ttccttcttt caaataacaa tgttttttat gtaagtaaat
    51121 cataaatcgt ttatttgtca actaaattaa aggattcctt tctttagtct agagaaattc
    51181 agtggtaaaa taaataagtg tgagaaaacg cattcgatgc atagatatag cgagaaatat
    51241 cgtgaaaatg gaggaatcag agtgaagtat gtcgaagtaa aaaatgaata caaacgatac
    51301 aaaatgggag aaacgaccat tacagcaaat gacgatattt ctttttcaat tgaaaaaggc
    51361 gaattagtga ttatacttgg tccaagtgga gcaggaaagt caacgattct taatatttta
    51421 ggtgggatgg atacacctga tgaaggtcag atcattattg atgacacaga tattgcgcaa
    51481 ttttcagata aacagcttac tgcttaccga cgaacagatg tagggttcgt tttccaattc
    51541 tacaatctcg ttcctaatct aacagcaaaa gaaaatgtgg aattggcaac ggaagtttct
    51601 cctgatgcgc ttgatcctgt agaggtattg aggcaagtgg gcctagctca tcgattgaat
    51661 aactttccgt cccaattatc aggaggcgaa cagcagaggg tttcaattgc tcgtgcacta
    51721 gctaaaaatc ccaaacttct tttatgtgat gaaccgacag gcgcgctgga ttttgaaacg
    51781 ggcaagcaag tattgaaatt attgcaaaac gctagcagac aacacggcaa taccgttttg
    51841 atcattacac ataattctgc tttagcgccc atcgctgata gagtcattca cattaatgat
    51901 gctaaagtaa ggtctgttga attaaatgat catccttcat ccattgatga gattgtatgg
    51961 tagggggatt ttatgaaaac aagaacatat cttaaagcag gttttcgcga aattcggcaa
    52021 tctaaaggac gattcattgc aatcatcctt attgtcatgc taggaacact tttatttgta
    52081 ggtgtcaaaa ctgctggacc tgtcatgcaa aaaacgatgg atcactatgt tggtacagca
    52141 ggactttctg atttgcaaat cgtttctact ggtggcttga cacaaaaaga tattgctcaa
    52201 gcagaaaaga tcccagatgc aaaagtagaa acaggaaaac agatctatta ttccaatact
    52261 ggcaaaaacg aagtgattca aatattttct tataacaaaa ataacaagca gaacaagcta
    52321 caattaactg atggaaaact cccggaaaaa ccaaaccagc ttgtattgga tgaaaaagcg
    52381 gaagatgaag ggtataaaat cggtgatatt tatcagattg attcagatga attgaaagaa
    52441 aaagagtata ccatcacggg ctttgtccgt tctccattgt tcatcaacaa tttggagaga
    52501 ggatacgcga acgtcggaaa tggtagcgtc gattattttg tctatttacc tgaatccgtt
    52561 tttaaaacag atatttattc tgtcttatat cttgatttta caaacgtaaa gaatttgaat
    52621 acgtacagca aagcttacaa agataaaatg gagcaaaatc aagaaaaggc agaaaagtat
    52681 ttacaagatc gacccgaaga acgcttagaa gagctgaaaa aatcggccaa cgaagaatta
    52741 gaaccggcac agcaaaaaat agcggatggc aaagcacaaa caacgcaagc aagaacacgg
    52801 ttagaagatg ctaaaaaaca gttagcgcaa cagtcagcaa ctatccagca gcttcctgaa
    52861 gcgcaacgaa caatcgctgt tcaaacatta tctcaacaag aagctcaact tgctgaacaa
    52921 gaaaggcaat tagttgagaa agaacgtgaa ctggcctctg cagaggaaga attgaatcga
    52981 aatgcagaaa aaattgaaaa tttaactaaa ccatcatacc tatacaatga acgttcagaa
    53041 aatgtcggtt ttcaagaatt tggtgatctg tctgatcgta tcgctgcgat tgccaatgtt
    53101 tttcctgttt tctttttctt tattgccgca ttgatcacct ttacgacaat gacacgtatg
    53161 gtagaagaaa accgcaggga aatcgggaca ttaaaggcgt taggttatac gaaagttgaa
    53221 atcgcaaaaa aatatgcaat ctatgcaagt ctagcctcag ggataggtat tgttttagga
    53281 acgatattag gaacgaatct tttgccaagg atcatttttg agctgtctaa cgagcggtat
    53341 gatattggga gtgcgcttat tttttatgac tggccgccga ttttacaagc agctgccgca
    53401 ttcttcattg ccgcatttgg cgctgcaatg atcgttttat tcaaagattt aagagaacgg
    53461 cctgctgcat tgcttcaacc gaaagcaccg aagccaggga aacgcatttt tcttgaatac
    53521 atcactccgc tgtggtcacg ccttagcttt aatcaaaaaa tcagttaccg aaatctcttc
    53581 cgatataagt caagaatgct tatggcgatt atcggaattg ctggatgtgc cgggctgatg
    53641 gttgcaggcg tgggattgaa agattctcta agttctgttt cagccaaaca atttggaccg
    53701 atcattgact atcaagcaat cgttacattg gattcatcca cggaagatag tcgtcaaaaa
    53761 gcagcggata tgctgcagaa gcaagaaaaa gtaaatgaga tcctagctat tcatactcaa
    53821 accgtagaat tgagaaaaaa aggacatgct tcgcaaagtc tgacagtgat cgtaccaagt
    53881 aaaacagctg atttggatcg ttttatccat ttaactgatc ttgaagagaa cccacgctcc
    53941 ttgcctaaaa caggaatcgc tattacgcaa aaagtagcag aactatttga cttatctaca
    54001 ggagatcata tttcgcttta tgatgaagat caaaaagaat ggaaagtcga agtaaaagaa
    54061 atcgtgaaaa actatctagg tagctttatt tatatgtccc ctgaatatta tgcttcagtt
    54121 actgatcaat cgatgatgga taatgcattt ttgctgcgta cagataagat gacaacaaaa
    54181 gaagaagaaa tgctttccga gaaactgctt gccacagacg ctgtgacaaa tacgtctttc
    54241 atgtccaaac aaatcgaaac gcaagaagaa tcaatggcga acttagactc ggttgtctta
    54301 atctttgtcg tattatcagg actgctggct tttatcgtct tgtataatct aactaatatc
    54361 aatatttccg agcgtgtacg cgaattatct acaataaaag tccttggatt ttttgataaa
    54421 gaagtcacga tgtacattgt tcgagaaaat atcatcttta ctcttttagg aatcatcgga
    54481 ggatttggtg taggatatat attgactgac ttcatcttgc aacaagcgtc tatggagaat
    54541 gtcatctttc cattagtgat cacatggtgg gcttatgtct tatcagcagg attaactatc
    54601 gtatttacgg ttattgtgat gattgttacc catttcaaat taaagcatat cgatatgatt
    54661 gatgcgttga aatcgaatga ataaaaacag aggttgtgaa atcagcggtt tgacctgagt
    54721 attagagaaa caaacggcaa aatagttttt catattttgc cgttttttga gctaataccg
    54781 aaggacagct gattgaacac catgaatcag aggttgtaaa aaagccgtcc tgcatcgagc
    54841 aaaaagaata ctcaaataaa attagctcct catgttttac atttgagaag actgattgcc
    54901 gtagataaaa gcttttgaac accatttata gaagaaacaa gaatattttg ctattttaaa
    54961 caagaggata gacaaataca tgtctgatct cttgtttttt attgggcaac agacctctta
    55021 gaatgtataa agataaattt tacaaacgct ttcggttcag ttataataat aaatgataac
    55081 ttgtatatat aagttgtggt aggtaataaa tcagttgaat ggaggtaaat atccatgaca
    55141 tttaaagaca aagtcattta ccaaatctat cccaaatctt acaaagacag taatggtgat
    55201 ggaattggag atttaaaagg aatcaaagaa aaactcccgt atctgaatga tctaggcatc
    55261 gacatgattt ggctaaaccc tatttatcca agccctcaaa aagacaacgg ttatgatatc
    55321 tctgattaca cagccatcga tcctatcttt ggtacgatgg aggaattcga acagctaata
    55381 gccgaagcaa agaaataccg gatagaaatc atgctcgata tggtgctgaa tcacgtttcg
    55441 acagaacatg aatggttcaa aaaagcacta gcaggggacc agcattatca ggacttcttc
    55501 attttaagag aagagccaac ggattgggta tcaaaatttg gtggaagtgc ttggtcgcca
    55561 tttggagata cggggaaata ttatttgcac ttatatgatc ggacacaagc tgacttgaac
    55621 tggcggaacc aagcagtaag agaggaacta ttcaaagttg ttcgtttttg gatggaaaaa
    55681 ggggtaaaag gattccgttt cgatgttatc aatgttatcg gtaaagacga acaattgaaa
    55741 aataataaag aaaatgatgg caaacctgaa tatacggaca aaccaatcac ccatacgtat
    55801 ctaaaagaat tgaaccaagc aacgtttggg acggattcca ctatcatcac agtcggtgaa
    55861 atgagctcta cgacgatcga aaattgtatc ttgtatacag aacctagtag agaagaatta
    55921 tcaatggtct ttaatttcca tcatttgaaa gtcgattatg aaaatgggaa taaatggtct
    55981 actcctccat ttgattttgc agaattaaag cgactatttc atacatgggg agaaaagatg
    56041 agtgagggta acggctggaa tgctctattt tttaataatc atgatcagcc acgtgcgctc
    56101 aatcgatttg tagctatcga tcagtatcga aaacaaggag cagagatgct agcaaccatg
    56161 atccatttga accgtgggac gccctatatt tatatgggag aagaaatcgg catgatcgac
    56221 ccagactatt catcgatttc cgactatatt gacgtagaaa gtttgaatgc ttataagtta
    56281 ctccttgaac aaggattcac agaaaaagaa gcgtttcgtc ggatcaaagc aaaatctcgc
    56341 gataactctc gaacacccat gcaatggtca gacacagaac aagcaggatt tactacaggg
    56401 aaaccttggt taaaagttgg aggcaagcta gaagagatta atgtagaaaa agaactatct
    56461 tctgaagatt ctattttgtc ctattacaag aaattgattc ggctgagaaa aacatatcca
    56521 atcgttgcac aaggagatta tcatgcttat gaagcaaatc atccggaagt ttatggcttt
    56581 cttcgccaat ttgaaggaca gcagctgctt gtattaacaa atttctatga taaagataca
    56641 aggatcgcca tcccagaagg attcacagaa gccaatattc ttatcgataa ttattccgat
    56701 actgttcttc atcaaagtct ggagctaaaa ccataccagg caattgcttt gctgaaaaat
    56761 taaaacaaaa acacaaaacg tcgttcctac gagctattct cgtaagatcg acgttttgtg
    56821 ttttatttaa aataatcttc ttcagaaaat gaagagtttt catactctcc tttatcgcca
    56881 taaaatttat tgtagcgttg tttgaattta cggaaaataa aagtaacgag taatttgact
    56941 aacgtataga taggaatacc aaaaataacg cccacaattc ccattagatc acccatgaca
    57001 agtaaaacga ttaagatcgt aatcggatgg atctgtaaac gatcacccat tactcgagga
    57061 atcaccaagt caccgtggag aagttgtacg gcaaaccaga caatgacaaa tttgacagcc
    57121 ataaaagtag agtcttggaa tgcaatgatc aaacctggaa agaaggcgat aaacggccca
    57181 atatatggaa tgatacataa caacccagca gaaatagcta aaacactagc aaattctaat
    57241 ccgatcagta agaaacaaac ccagtagact gctccaagga taaatgaagc aatgatttgc
    57301 ccctttagaa atgaacccaa ttgttgatta gcaatctttg tcaaattttt aaaatctgta
    57361 cgaaatgctg gtgggacaat cgctaaaact gactgataaa atttttgagg atctctcaaa
    57421 aggaaaaaag cgacaattgg accagtaaat aaagtaataa aaatagctgt aacagttgaa
    57481 aaaatgttgc tgatccgttg tgcgccactt tcccaataat ttccaataaa tccagctata
    57541 tcagacgtca cgtcgtcaat agaagcgaaa aattgattaa atgaatcggc aaaaggtgtt
    57601 ttctccaaaa attgtcggag actctcttga aaatcttgaa acaaatctgg caaactactg
    57661 atcaatgtct ttgcctgttc ttcaagaaaa ggaataagcc atatgcttcc aagccccaat
    57721 aaaaataaaa taatcacata aacgataatg actgacaagt tcctcgaaag gtgcttgctc
    57781 aaacgtttga tgactggttc caaaagataa tacaagatca gtgcaaacaa taatgggggc
    57841 aagatagtag cgataatgat tccgacaggc ttgaatataa acgagatctg atggaaaaaa
    57901 taaatgatca gcgcaagtaa gataactatt cctaaaacat agtaagaggt ttttcctcct
    57961 aaaaagcgga tcaaacgatt cgttgtatct gtttcttttt tcactctttt tcctccttta
    58021 tctataactt cattatagac aaaagcgggt ataaaaggaa aatgatagag gttgattgca
    58081 aaaatatcgt caatcattca tataaacttt ctcatcaatt aaaataagaa aacgcttgac
    58141 agttctttta accatatgat aaattaaatc ctgttgctaa aacgtaacta ttacggtttg
    58201 cgaaaagcga atcggaacat tcaaatgaaa gaagatgaga agaggagtgt cataaatgaa
    58261 acgattgata ggtatattgg ccatgctggt tttagcaggt atgttaactg cttgcggggc
    58321 aagtgggaaa gccgaggaca gtaaagaaaa attgagcgtg atgacgacct tttatcccat
    58381 gtatgatttt acaaaagcaa tcgtcgggga tgaaggagaa gtagagttat tgattccagc
    58441 agggactgat tcccacgatt atgaaccttc tgctaaagac atggcaaaga tccaagatac
    58501 agatatcttt gtttataatg atgaaaacat ggaaacatgg gtccccgcaa tccaaaaaac
    58561 attacaagaa ggcaatgtcc atacgataaa agcgacagaa ggtatgcttt tacttccggg
    58621 aagtgaagaa ggacatgatc atgaccatga acacggggaa gaaggccata cacatgagct
    58681 agacccacat gtatggctag ctccaagttt agcaatcaag caggtagcaa acatacgtga
    58741 tcagctgatt gaagcttacc cagaaaaaca ggaagtatgg acaaaaaatg cagcagccta
    58801 taccgagaaa cttcaagcac tccatcagct ttatcaagaa acctttaagc aagcaaaaca
    58861 aagatctttt gtcacgcaac atgctgcatt caactatcta gcgttagaat atggattgaa
    58921 tcaagtatcg attgctggtc tttcttctag tgaagaaccg tctgcggcga gaattgctga
    58981 actgaaacac tttgttaaag aacatggaat caactatatt tattttgaag aaaacgccaa
    59041 agatagtatt gccagaacgt tagcaaacga agcaggtgta tctttagaag tattgaatcc
    59101 tttggaagga ttgacgaatg aacaaataga aaatggagaa aactatcttt ctatcatgga
    59161 agcaaacctc gaagcgttga aaaaaacgac agagacagaa aacccgctag aagatacttt
    59221 gacccccaaa aaagaaaaga gcgtctacaa cggttatttt gaagacgagg atgtacaaga
    59281 tcgctctctt tcagattggg caggaacttg gcaaacagta gatacgtatg tagaagatgg
    59341 gacttttgat cctgtttttg aatataaaga aaaattgaat caggataaga cagcacaaga
    59401 atataaagaa tattatacga agggctatca aacagatatt gcttcaatca agatcagtga
    59461 gaaagagatg gcatttacgt ttaagaatgg aacaacgaaa cgttcaaatt atcgttatgt
    59521 agggaaagaa atcttgacct acgaggcagg aaaccgtggc gtaaggtatc tattcgaagc
    59581 agaagataag gaaagtggcg cttttagata tattcaattc agtgatcaca cgattgcacc
    59641 ggctgactcc gaacattttc atttatatgc aggaaatgaa agtcaagaag tattgctgaa
    59701 agaaatggag aattggccaa cattttatcc agatacaatg gatggacgtg aaattgcaca
    59761 ggaaatgctt ttgcactgat aggcaaaaaa gaaccgaagg gtttggatcg gctggtcatt
    59821 tagtcggtcc aaactattcg gttcttttta tgaaatcaat ttctttaatc cacggataga
    59881 ggctgggatg gtctcgccgt ttgccaacgt gacttctctg gttttttatt aatcgaccgc
    59941 acattttgtg tattgataac ataggattta tgacaacgga taaaattccg atgcattttc
    60001 tcgatttctt tgatcttctc atagaattgt aattgggcat gagtcaaatg gacttccagt
    60061 ttatgtggcg ctcctagcga agtagaaaaa tattggatgt ctttgatcgg taaaaatttg
    60121 atctcatcat taaactctat tttgaagata tctttttgga tttgggacat ttgaaaatct
    60181 tcgaaaattt tgctcaggat atgctcgaca ttttctttta aatcataaag atcttctttg
    60241 acgatataat cagtcggttc gatgttgctt tttaagattt tcatcgacat ttccgtatgg
    60301 gaagtcacat agattatttt tcctaaagga tctaattcac ggatcaattt accgatcgct
    60361 acaccatcca tttcactgtc ttctagttcg atatctaaaa agtaaatacc aaaagaagaa
    60421 gttctgatct tggctgcatt gagaagttct tccggatcag aggtagaaat atggacataa
    60481 ctgtctaagt tttcaatcat gattctattt ttgatcaccg ttgataaaac ttctaattgt
    60541 tttggatcgt cttcacaaat ataaatggga atcatatcat tactccttta ttttatttgt
    60601 atataaatcg tttgagaaaa acgctggttt tcaattgatg tttcaaggaa aagataagga
    60661 ttggtctttg tcaattcatc caagataaac aagccaaggc cttcgttttt tggatttgtt
    60721 tttgttgaga accctttttg tttcaactgg tttaacggta atgtatttgt tgttgtattg
    60781 atcacacgaa tcatcacgga atgattcttc ttgagtatgg tcaattcgat ttttttcttc
    60841 tttgcagcac tagctccttc aatcgcattg tccaagataa taccaagcat acgtacaagg
    60901 tttaccgtat gtttcgtatc cagttcgatt ggctcaggaa aatgaagctg gacgatcaac
    60961 cctttttcct gtgccatcat gagttttaat gacagtaaac tacggatctc aggaactttc
    61021 atattatcca acatacttag attggccgga aaaaggttca tcatctggcg tgtaggtaat
    61081 atcgtttgat cataataaga ccgtaatcct tctaagtcgc cttctgaaat atagctgtga
    61141 agagaaaaaa gcagattttg gtaatcatgt ttgaattctc tcaagtcttg atagtttttt
    61201 tctagatctt tgatataatc ctgttgcatc ctgacttgtt gctctaataa ctgatcagct
    61261 aatttcttat tagagatttt ccaataataa accaagatga tcaggccaac acacagatat
    61321 agaatgaaaa aactcgtatt caaagctaag acatcaggtg tttcaccaga aaaacgggta
    61381 taaaaaataa tgatatagta tgtaaataat atcaatgtcc cgatcgtacc gatcatacgg
    61441 cggtccacag tgtgaaacca agtctctaac gattttttta aacaaaaagc taataaagca
    61501 gataaaaaag cggataagac aatgtgaaaa tactgcaatg aatctcctgg ttcaataacc
    61561 gaagatttca aaacaaaaaa atcgccgatg cttgttaagt gatctcctaa aagcgacgtc
    61621 aataacccaa aaggaagcgc gtaaaccaat tgttgacgcg aagtcgaaaa gaagaaaaaa
    61681 gtaagcatca gcattcctat agcaattaga gcagtaaaaa tccctaaaat cgaatagacc
    61741 catccaatca aaatacaaga aatcagaatg actcccgcaa acagatattt gtatcggatg
    61801 tgatggtcaa tcaataagat acaaattgtg taaatcaaat attgcaacgc tatggaccat
    61861 aaaaacataa agtacagctc ccattgattt ttaccaaatt atagcacaat tggcgtatta
    61921 tttttttaat ttttcaggga ttttgaattc agaactcata ccaaagcagc agccagatac
    61981 aaaaaagtca gataagttag ttaattgttt aatgattgtt tttttcatga aagattcctc
    62041 caaaattcat caagtagttt gtaataggaa gcaacgtagc tatttctaaa acaatcccaa
    62101 gtagcaaata ggtagaaaag taagaagctg agaaaaataa cgtaatgaga gaaaaaatta
    62161 gaaacatgct gatcgccttc tttaataatg ggggacgttt ctcgggttca atgggattga
    62221 ttgctgtacc tcttgatcca tagcgaagca gtaacaaaaa gcagctgatt aacaaaaaga
    62281 ccatcattgg gaaggagata gtcaattgca gatgtttcat caccaaggga acgccccata
    62341 aataaagtag tgtccacagc agacaaacga attcagagtc ggaatgcaat ccataagcat
    62401 atctgcggat gcagatataa gaaagaaagg tgattcctgt ttccgcccat gagcctgtca
    62461 ccagagaaaa gactagaacg accgctagtt ttccaagatt attcaataaa acttctaaga
    62521 caaattttac ttggacatac accatatctt cttccgattt gtctccgctg aataaggctt
    62581 ctaataattt gttcacaact ttatctttca tctcatacgt acctccacca caaattttat
    62641 gctaaaaaaa ataaaaaacg tcttttgttt cataaaaagt ggttttgaca tcaaaaccgt
    62701 catgatctcc taaaaaaatc ttcgttttcc aagaacctta ccaaaaaaag tgacagtttt
    62761 gatacgaaaa gtacttttta tgacaagttt gtgctttttc gagtgattta tgttaaaaaa
    62821 ttactgacct tataaaaggt gatggaggga ttcttatgga aaatgaacaa ttgtttgaag
    62881 tcggcgaaac atatgcattt aaatctgatt tatttgaatc accaataaaa ggaaaaatag
    62941 aaaaaatcta tgaaaatagt gtgatgatac gtgtcatgaa atgcgcagta gaagacaatg
    63001 ataccgctca taatctgcac tggcagttag ttgtacgcaa atcaagtaca atagaagaat
    63061 aaaaacagat aaaatgttga gagcataaag aggttgtgag tctcccgttt agctctgagt
    63121 tacaaggaat aatgttaaaa aacagctttt catgttttat gaatattgcc ttgtgaccga
    63181 ggtgttggca ggagaatact gtttatttga aaaaagggct gcgacaagac ttttgtcaca
    63241 gcctttattt tcatgaatag gagttacttt tggctacttt tagagaagta gcttttaagg
    63301 agcagataaa gtcaaggctt ttatttctat aaatgaagta gtaaaatgaa gaacagattt
    63361 tattacagga aggatgccga taattgtgat cattgttgca ggaacgatcg gtgcagggaa
    63421 aagcacacta actgaaatgc ttgcccaaga tttagaaaca aaaccttttt atgagaacgt
    63481 ggaagataat gaagtattgc cgctatttta ttctaatcca gaaaagtata cttttttatt
    63541 acagattttc tttttgaaca aacgcttttt ggcaatcaaa gatgctttca gccatgatga
    63601 caacgtacta gaccgctcca tctacgaaga tagtatgctg tttcatttga atgcagattt
    63661 agggcgtgtt tctgaagtag aagtaaagca gtatgaaggt cttttagaaa cgatgctgaa
    63721 ggagttagaa gaaatttcgc cacaaaagaa accagatcta ctggtttata ttcgtgtatc
    63781 ctttgaaact atgttagcga gaatcaaaaa aagaggccgg gaatatgaac agctagaaca
    63841 agatcctgaa ctctattcct actataaaga actcaatcga cgatacgaag agtggtatga
    63901 acagtttgat atatgtccca aaatagtgat cgatggggac aaatatgatt tcgtagccga
    63961 tccggcatgt ggagaacaaa tcgtacaaga aatcaagatg cgggcaaaga aaatgacaga
    64021 agaggcagat gcccagtcga tccgctacac tacaaataac ccgaaagcaa agggaatctt
    64081 tacaactaaa taatataaaa actaagcaaa ttgctcactc gcagatattt ctctgtgagc
    64141 agcatcttgc ttagtttttt tgattcattg agatcaaata gtgaaccgat tcgtatgata
    64201 aaattgccat aaatgaaatg tctgagtgag ggccatgatc aaaaataaaa tggaaacgat
    64261 caaaatcgaa tgggcgccaa acagatgatg acagaaaaca aggacaatag ctcctgcgat
    64321 aaaggcaaga acgacactta gatagtggaa aaatcgatca aaatctgctt tttctttaga
    64381 aatacaataa tcatataagt tcgttgctac cccttttaaa tttccagttg taaataaatt
    64441 agtgtaggca cgcccgttga ttttatcaaa agctacccac tggatcgatg cagtaaaagc
    64501 aatcaaaatc gttactaatg cgggatgctc atggacgggt gtcaaactaa caaagaaaaa
    64561 aatcactgct tcataatata gaacaaacaa gcgccagaaa tgtttttctt tttttctaaa
    64621 ataatcaatt agatactttg ttagaagaat ccctaagaaa aagaaaaggg tagacaatac
    64681 ttttttacca acacttagcc attctccgtc aaatgcttgg atgatcgata gaatgatatt
    64741 gccagtttga gcagaggcaa aagcaccata ttggatataa gtataactgt ccatggctcc
    64801 tccgataaat gtcagcaaaa gtcctattgt acgcccctcg tgaaaggaag tctgcatttt
    64861 tttcatcacc ttttatgaat atttttgtct tccttatctt agcataaacg attgaaaaaa
    64921 aggaaaaatt ttcagaaaac taaaatcata tcgcttccca ttttcatttt tagaaaggtc
    64981 tgttagtata aagaaaaaat agacaacgaa actttaaata accaacttga aataaacaaa
    65041 ttcaaagaag aaagatggga aggaaatcaa tcagttgaat gaaaaagaaa ttctccgatt
    65101 acaaaaagaa ttgaaacgct gggagaacaa gtataaaaag cttcaacaag tctttcggct
    65161 acaagaagaa atcattgcta cctatgaagc ggcttatggc gaagcacccg gacttattga
    65221 ggaagaagta caagtaaaag aaagtgaagg aaacagctct ttctttaaaa tgatggagaa
    65281 acgttcctat aatcgcttca atcaagaaca aaaagcagct atcacatatg atatgaagaa
    65341 acatcttcga atcatagcag gagctgggag cggcaaaacg caaacaatct gtgcaaaagc
    65401 tgtttatctg atgacgcaaa aacaagtaga cgaagaacgc atactaatga tcacttttac
    65461 aaggaacgct gcaaatgaac taaaaaaacg agtagataat ttcagccaga gaaaaaccgc
    65521 tgtccatatc ggaacattcc atagtatctt ttttaggata tacaatgaaa tttgtcgaaa
    65581 atttcctgaa gtagcaatgc aaggaataca aggagacttt tcaaaggata ctgcacaaaa
    65641 agtgaatgct gttttgcaac agctgatacg taaatataat ctttatatat ttgatcaata
    65701 tggagaaaaa acgattgctt ctcggttaga ctattggcag aatatgaacc tttcagtaga
    65761 agaaatggtt cagttagtta aagaaaaata tgattccatt gataagaatg cacgacagcc
    65821 gatatcagaa cgactgtatg gattgctcac ggaactccaa gaacaaaaaa gaagccagca
    65881 attgctagag ttcaatgatg tactccaaaa tctgaaaagc gctttggaga atgaggagat
    65941 ccagcattat atcggccaaa aatatgatta tctattcatt gatgaatttc aagatactaa
    66001 tccacttcaa tgggaaatcg tgaaacggat gacaaaggaa aacaggatca agttgattgt
    66061 tgttggagat gatgatcaga gtatctatgg attcagagga tcagagccag catacatcaa
    66121 gaactttgaa aaagaatatc ctaccaaaac tcttttctta ttaactaatt atcgttctcg
    66181 ggcagctatt gtccaaggag caaatagatt gatcagctat aataaaaatg atcggatacc
    66241 aaaggcgatg attccagcac aaaaagaagc aggtgttatg gaagcctatt tgttttctga
    66301 tactaaagca gaaagtcagt ggctaatcaa acaaattcag acatttattc aaaaaaatgg
    66361 gaaatataag gaatcaatca tattataccg atcagcttca caaacgcagc agcttgtcca
    66421 atctctgctg aaaacgaata ttccttttgt actggaagca gatagtccat atgaaggtat
    66481 ctttggtatc aagtctttcc aacatttcta tcaaaaaatt gttcattggc aaacagcact
    66541 gaatctgcaa aagaaaaatc aggcctacca gcaattgctc cggcagctga tggcagactg
    66601 ctacctgaaa aaaagtgaat gcgatcaata cttttctcct aaatatcaaa atatagccgt
    66661 tgcagattat attttgtcga taagccccaa tttaaaatcg aaatctgcag aaatccgaga
    66721 gtttgaggaa gcgttgcgta aaacagacca gcatcaagaa atctattcgc ttgttcaaag
    66781 ctatcttcat ctgccacgta tagctaagga aatcgaccag agtgatcaag aatggatacg
    66841 agaagaagta aagcagcatg ctactttcag aagactccaa gccttatcgc aagaaaccca
    66901 agcgacagct aaagaattaa aaacccgctt gattgcttac agacaaggaa aacttgatgc
    66961 actatgtatc cagagcattc acaaaagtaa aggtcttagt taccgaaatg tctttttgat
    67021 tggttgtaac gaaggaagtt tgccttataa cggagcgaca gaaaagaaag taatcgactt
    67081 taatgaaata aaagcagaac cagtaacgac gatagaagaa gaacgaagac tattttatgt
    67141 tgcgatgact agagcaagaa atcgtctgta tctatgtgta cctcaaatga aaaattcaag
    67201 aaaattgaaa gtatcgagat ttatcaaaga aaccgggagt cgtgtgaaaa aaggaaagtg
    67261 atagaagaga gggtgtgaca gaagtggtca gtttcaagaa agaagaagga atgtacgaaa
    67321 attgcttctc aaatttttgt gaaatttcag cttattttcg aagaagctat ttctggaaca
    67381 cagtttattc ggaaaaggga ggtgtgacaa ga
//
LOCUS       DAXWLF010000002        41953 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXWLF010000002 DAXWLF010000000
VERSION     DAXWLF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.002"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            57..716
                     /locus_tag="ACGE4U_000057"
     CDS             57..716
                     /locus_tag="ACGE4U_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8392260.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            801..1706
                     /locus_tag="ACGE4U_000058"
     CDS             801..1706
                     /locus_tag="ACGE4U_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8392261.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(1708..2340)
                     /locus_tag="ACGE4U_000059"
     CDS             complement(1708..2340)
                     /locus_tag="ACGE4U_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFF8392262.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    2491..2616
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2660..3184
                     /gene="infC"
                     /locus_tag="ACGE4U_000060"
     CDS             2660..3184
                     /gene="infC"
                     /locus_tag="ACGE4U_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFF8392263.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3256..3456
                     /gene="rpmI"
                     /locus_tag="ACGE4U_000061"
     CDS             3256..3456
                     /gene="rpmI"
                     /locus_tag="ACGE4U_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFF8392264.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3509..3868
                     /gene="rplT"
                     /locus_tag="ACGE4U_000062"
     CDS             3509..3868
                     /gene="rplT"
                     /locus_tag="ACGE4U_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFF8392265.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            4120..4585
                     /locus_tag="ACGE4U_000063"
                     /pseudo
     CDS             4120..4585
                     /locus_tag="ACGE4U_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            complement(4752..5939)
                     /locus_tag="ACGE4U_000064"
     CDS             complement(4752..5939)
                     /locus_tag="ACGE4U_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFF8392266.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            6045..6932
                     /locus_tag="ACGE4U_000065"
                     /pseudo
     CDS             6045..6932
                     /locus_tag="ACGE4U_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            6980..9109
                     /locus_tag="ACGE4U_000066"
     CDS             6980..9109
                     /locus_tag="ACGE4U_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFF8392267.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            9084..9773
                     /locus_tag="ACGE4U_000067"
     CDS             9084..9773
                     /locus_tag="ACGE4U_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392268.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(10181..10867)
                     /locus_tag="ACGE4U_000068"
     CDS             complement(10181..10867)
                     /locus_tag="ACGE4U_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8392269.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(11003..11197)
                     /locus_tag="ACGE4U_000069"
     CDS             complement(11003..11197)
                     /locus_tag="ACGE4U_000069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392270.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(11247..11687)
                     /locus_tag="ACGE4U_000070"
     CDS             complement(11247..11687)
                     /locus_tag="ACGE4U_000070"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFF8392271.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(11691..12536)
                     /locus_tag="ACGE4U_000071"
     CDS             complement(11691..12536)
                     /locus_tag="ACGE4U_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFF8392272.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            12685..14103
                     /gene="pepV"
                     /locus_tag="ACGE4U_000072"
     CDS             12685..14103
                     /gene="pepV"
                     /locus_tag="ACGE4U_000072"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFF8392273.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(14179..15381)
                     /locus_tag="ACGE4U_000073"
     CDS             complement(14179..15381)
                     /locus_tag="ACGE4U_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392274.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(15368..17155)
                     /locus_tag="ACGE4U_000074"
     CDS             complement(15368..17155)
                     /locus_tag="ACGE4U_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFF8392275.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(17168..18121)
                     /locus_tag="ACGE4U_000075"
     CDS             complement(17168..18121)
                     /locus_tag="ACGE4U_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8392276.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(18306..18656)
                     /locus_tag="ACGE4U_000076"
     CDS             complement(18306..18656)
                     /locus_tag="ACGE4U_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFF8392277.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            18856..19935
                     /gene="pepA"
                     /locus_tag="ACGE4U_000077"
     CDS             18856..19935
                     /gene="pepA"
                     /locus_tag="ACGE4U_000077"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFF8392278.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            20078..20398
                     /locus_tag="ACGE4U_000078"
     CDS             20078..20398
                     /locus_tag="ACGE4U_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8392279.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            20420..20884
                     /locus_tag="ACGE4U_000079"
     CDS             20420..20884
                     /locus_tag="ACGE4U_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8392280.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            21080..21685
                     /gene="ytpR"
                     /locus_tag="ACGE4U_000080"
     CDS             21080..21685
                     /gene="ytpR"
                     /locus_tag="ACGE4U_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFF8392281.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(21724..23013)
                     /locus_tag="ACGE4U_000081"
     CDS             complement(21724..23013)
                     /locus_tag="ACGE4U_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFF8392282.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            23441..23920
                     /gene="rlmH"
                     /locus_tag="ACGE4U_000082"
     CDS             23441..23920
                     /gene="rlmH"
                     /locus_tag="ACGE4U_000082"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFF8392283.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            24273..24587
                     /locus_tag="ACGE4U_000083"
     CDS             24273..24587
                     /locus_tag="ACGE4U_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFF8392284.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            24603..24989
                     /locus_tag="ACGE4U_000084"
     CDS             24603..24989
                     /locus_tag="ACGE4U_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFF8392285.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            25018..26403
                     /locus_tag="ACGE4U_000085"
     CDS             25018..26403
                     /locus_tag="ACGE4U_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8392286.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            26406..26559
                     /locus_tag="ACGE4U_000086"
                     /pseudo
     CDS             26406..26559
                     /locus_tag="ACGE4U_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            26582..27787
                     /gene="mobT"
                     /locus_tag="ACGE4U_000087"
     CDS             26582..27787
                     /gene="mobT"
                     /locus_tag="ACGE4U_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFF8392287.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            28571..30481
                     /gene="ltrA"
                     /locus_tag="ACGE4U_000088"
     CDS             28571..30481
                     /gene="ltrA"
                     /locus_tag="ACGE4U_000088"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFF8392288.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKRNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            30585..30809
                     /locus_tag="ACGE4U_000089"
     CDS             30585..30809
                     /locus_tag="ACGE4U_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392289.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            30926..31423
                     /locus_tag="ACGE4U_000090"
     CDS             30926..31423
                     /locus_tag="ACGE4U_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFF8392290.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            31510..31902
                     /locus_tag="ACGE4U_000091"
     CDS             31510..31902
                     /locus_tag="ACGE4U_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8392291.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            31886..34333
                     /locus_tag="ACGE4U_000092"
     CDS             31886..34333
                     /locus_tag="ACGE4U_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8392292.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            34336..36519
                     /locus_tag="ACGE4U_000093"
     CDS             34336..36519
                     /locus_tag="ACGE4U_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFF8392293.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            36516..37517
                     /locus_tag="ACGE4U_000094"
     CDS             36516..37517
                     /locus_tag="ACGE4U_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFF8392294.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            37532..38446
                     /locus_tag="ACGE4U_000095"
     CDS             37532..38446
                     /locus_tag="ACGE4U_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8392295.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            38760..38807
                     /locus_tag="ACGE4U_000096"
                     /pseudo
     CDS             38760..38807
                     /locus_tag="ACGE4U_000096"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            38823..40742
                     /gene="tet(M)"
                     /locus_tag="ACGE4U_000097"
     CDS             38823..40742
                     /gene="tet(M)"
                     /locus_tag="ACGE4U_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFF8392296.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            40840..41850
                     /gene="tet"
                     /locus_tag="ACGE4U_000098"
                     /pseudo
     CDS             40840..41850
                     /gene="tet"
                     /locus_tag="ACGE4U_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
BASE COUNT        13640 a         7624 c         8586 g        12103 t
ORIGIN      
        1 tttaaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga
       61 gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg
      121 ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct
      181 tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac
      241 gttttgcaag catcaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt
      301 atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag
      361 tttgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata
      421 aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg ttagagcgta
      481 tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga cccaagtcta tacgattatt
      541 acaaagaatt gaacagacgt tatgatcaat ggtatgaaga atacaaagaa agtccaaaaa
      601 tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct
      661 tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtatcc atttgattta
      721 cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac
      781 aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt
      841 ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca
      901 ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg
      961 attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata
     1021 atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta
     1081 ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa cggatgccag
     1141 acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc
     1201 ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa
     1261 caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta
     1321 caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcactggct atttatcgca
     1381 agttgtcaga aaaggaaaaa gaagagttgg acgatgcact gatcgaagca cgacaagcag
     1441 ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt
     1501 tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga
     1561 ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt
     1621 ccgttttcgg atgggaaatc gccttgatca tcagtttggt cttatctatt tggatgctta
     1681 ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc
     1741 gatcacaagt gcttgctctt gattgacgat tagtcccgcc aaagaatctt gagataaagc
     1801 gattgttgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt
     1861 cgaaccggtt aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc
     1921 gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga
     1981 agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt
     2041 cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc
     2101 cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaacc
     2161 tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc
     2221 gagatagctt tggttacctc cgttgatcag aagaccagag aagatctcct catcttctaa
     2281 ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat
     2341 gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg ctagaaagcc
     2401 tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag
     2461 attagttttt gctatactat aatggttgag aaataaaagc agaagcaccc gcttctcgcc
     2521 ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg
     2581 cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc
     2641 tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg
     2701 tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc
     2761 agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc
     2821 gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa
     2881 agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc
     2941 tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa
     3001 gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat
     3061 tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca
     3121 aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa
     3181 gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga aagattttga
     3241 ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa
     3301 cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc
     3361 aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc
     3421 aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa
     3481 gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg
     3541 taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt
     3601 atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg
     3661 tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat
     3721 gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa
     3781 ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga
     3841 ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac
     3901 gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca
     3961 ataacgtata agtctaaaat aacataatta aggtttttta attgaaaact gaacatttat
     4021 ggtcatcctt taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta
     4081 gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt
     4141 attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg
     4201 atggtcggct tttgtcatca tggctactgt atggatggga ttagcgctag tcggaaacac
     4261 gttttcacca aactcggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta
     4321 tacgacgggg ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt
     4381 tttcgggcgt gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga
     4441 acttggaggg gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat
     4501 attcatgtcg atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccgat
     4561 cttgatgtca ttaggtattt cagtcaagtc cacaatcttg tgtaaaagtt caacctcatc
     4621 aaaagcacaa tgttttgcgt tgtgaaccta actgccatgc ctttctttct gaggagagag
     4681 gtcaggagca gtagcgcagc gactcttgat gtctctcctc agaaagaaga gactcgcaca
     4741 ggttcatggt gctaaatgtt attcgaatta aattctaaat attttcgtgg agaagccagc
     4801 cactcagtgt ctttttccat caaaagtgtt ccaattatcc gagtgatgga attgatattt
     4861 ggaaaaatcc gaatcactcg ttctctccgg cgaatctctt cgttcaaccg ttccaacaca
     4921 ttggttgtac gaatccttcg gcggatattt tcaggaaaag acaaaatttg aatagcatct
     4981 tcaaagccgt tttctaaaat gtcgcaagca gcacttaatt ttgaatcaca accatatttt
     5041 tccaagaagt gctcttttct ttcacgtgtc aattcaagtt cagaggcttt aaagatactc
     5101 ttcaactcgt cttttacatc agaagaaact ttctttggca gcttatcaaa aatatttctc
     5161 aaaaagtggg cttggcatct ttgccagctc gcacctaaaa agttcttgcg aacagcctta
     5221 actaaccctt tgtgtgcatc agaaatgacc agttttacac cgaacaaacc tcgttgaacg
     5281 aacgattgaa aaacggtatc ccaattatct tccgattcgc cgtcttgaac atcaaaacca
     5341 agaatcgtac gtcgtccatc agaatcaatg ccgataccaa tatatacacc ttttgagaca
     5401 atacgatgat tttcccttac tttcatatac agggcatcac acatgaaaaa tggatattcc
     5461 gagccttcta ggctccggtt tctccactca aaaacaaatg gatccaactg tttcatcaga
     5521 tttgaaacga atgatttaga aacagtcgca ccatccgtta gcagttcaac tgttttagtt
     5581 actttgcgag tggagacacc agaaatgacc atttcaatca tggcagcgat caaagctttt
     5641 tcgcttcgct gatatctttc aaagatagta ggactaaact taccgtctct ggttcgtgga
     5701 acatgaagtt ctaacgtacc aattcgagtt gtaaacgaac gatcgtagta accatttctt
     5761 tgtgattgcc gttcttctga acgttcatag gcatttgcct gaatatattg atttcgttgt
     5821 tcttccatca gctgattaaa catgatagtc ataagtgttt ttgcggtatc attcgcaccg
     5881 ctttcgttta taattgcttg aatttcttcg ctttcaaaag taaaatgtac ttgagtcatt
     5941 gatatcagtc ctattcgagt atgttttctg caaaaaacat tgtactccta actgagatca
     6001 atggcttttt tgatgcttca cttttacaca attatatgga cgtaatcggt atttcagtac
     6061 cggttgcact gttctcattt atgggctcga ttatggctgg aatctttgct aatatcgtca
     6121 atttcaaaca atatcaaacg atttatgctg gtttcaatcc agcggctgaa agctatactt
     6181 ataacgacta tttccaaatt ggtatgattg ggatgattgt aagcttagtt gtggtactaa
     6241 cggtcgctaa tatatcaatg aacaaaaaga aacgctatgc aatggcagca aacgtaccag
     6301 ctgaaggtgg cgacgcacca atgatctcat ggttagccgt attattacca gttttagggg
     6361 tcgttctttt agatcttcaa atcatcttag gttttattct agcaggtatc tgggcattgc
     6421 tgtttactgg aaaattgcgc ggaggttata aagaaatctg tcgtcagttt gcgaaattat
     6481 ttacagatgg cgcagtcgat gttgcaccaa tggttggttt cttgatgacc ttagcgatgt
     6541 tcaataattc agctgcgtat gcttctcctt atttttcagc tatttttgga gattggatac
     6601 cgcaatcacc attagtcttg gcaatcgtat ttgctatatt gacaccacta ggatttttcc
     6661 gcggtccaat gaatctagtc ggttcaggct cagctatttt agctgtcgtg ttagctgtta
     6721 atccaacgat gtcgccagcc tttttgttcc cgttatttgc aattacaacg attgctcctc
     6781 agcatttaga tattacacaa tcttgggttg cttggggatt aggttataca aaagtaactt
     6841 ctcgagaata tatgaagaaa tccatcccaa ctggttggat catcggtgcg atcttatgcc
     6901 tgatcacgtt cttattgtac gggaacgcct agtaaacaaa acgtcgataa aaacgattag
     6961 ataaatggag gaaaagaaaa tgaaacgttt tgtgcgaatg ggaattgatg ttggcggtac
     7021 acatacgaaa gcagtcgcaa tcgataatgc gacgcacgaa atcatcggaa aatcttcagt
     7081 gaaaacaacc cacgatgatg tccgtggagt agccgcaggt gtcgttcagt cttttcaaaa
     7141 ttgtttgcgg gaaaataata tcagtccaga agatgttgtg tttgttgcac attctaccac
     7201 acaagcgact aatgcactga ttgaaggaga tgtagcaaaa gtcggcgtaa tcgggatggc
     7261 aaaaggtgga ttagaaggtt ttttagcaaa acgacaaact agattgaacg atatcgattt
     7321 agggaataag aaaaaaatag agattgtcaa tgcattctta cctgtcaaaa acttaaatgt
     7381 agaccgtgtt tcagaaacga tttcttcttt agaaagagaa agagcggagg tcttggtttc
     7441 ttctatggca tttggagtag ataatgggga accagaacga gttgtttatg aagcagcatc
     7501 tgtgaaagcg atccctacaa cgatggcttc tgacatcaca aaattatatg gattgacaag
     7561 aagaactcgg acagcagcaa tcaacgcatc tattttacca aaaatgctag atacggcaac
     7621 ctcgacagaa gactccgtac gagaagcagg ggtcaatgtt tctttgatga ttatgcgcgg
     7681 agacggcgga gtaatggaaa tcaatgaaat gaaaaaacga cctgttttaa ccatgctttc
     7741 cggtcctgct gcatcagtca tgggctcttt gatgtacttg agagcgtcta atggtgttta
     7801 ttttgaagta ggcggaacaa caaccaatat cggggtcatt aaaaatggac gtccagcaat
     7861 cgattattca attgtaggtg gccacccaac atatatctct tcattagatg ttcgggttct
     7921 aggtgtggca ggcggttcga tggtacgggc taatcagtca ggaatcatcg atgtcggtcc
     7981 acgttctgca catattgccg gtctagatta tgcagtattt acggagacag aaaaaatcaa
     8041 aggtcctaag gttgaatttt tctctcctaa agaaggagac cctgctgatt acgtcaaggt
     8101 agtgatggaa gacggagaag aagtgactat tacaaataca tgtgcagcca atgttcttgg
     8161 attagtgcaa gaagaacatt tttcttatgg aaatgtccca tctgcacgaa aagccataca
     8221 ggcattagca gactattgtc atacgacggt tgaagacatc gcagagcaga tcatggaaaa
     8281 atcctatgct aagattgaac cggtgatttt agaattggca gacaagtatc acttggagaa
     8341 agatcagatt tctcttgtag gtgtaggggg cggcgctgcc tcattgatta cttactttag
     8401 taataaaatg ggtgtgaaat attcgattcc tgaaaacgcc gaagtgattt cttctattgg
     8461 tgtagcactt gcaatggtcc gagatgttgt agaacgtatc atcccttcgc caagtaaaga
     8521 agatatacga tcgttgaaaa atgaagcgat gaataaagcg attgaatcag gcgctacacc
     8581 agaatcgatc gaagtccacg tagaaatcga tccacatact tcaaaagtaa cggctatcgc
     8641 tactggttct acggaagtga aagcaaccga tctaaccaag gagatcacaa cagaagaagc
     8701 actagagctt gctgcagagg atatgcgatt aaataaaaat gaagtttgct tattggaaaa
     8761 tacacctttc ttttatgtat gcggcgaaca gaatcgttca aaaaatgctg gaagtcttcg
     8821 gatcatcgat caaaaaggat ttatcaaggt ccaacgtgga catgcttcat gtatgaaaac
     8881 aactgcagcc aattatatga cagctgtcga acaactttgg gaagatatgg cagtttatca
     8941 aactgaattg attgcccgac cagaatttta tctctgctta ggagcaagag tctctgattt
     9001 tacagcgacg gatctggaac aactacagct tttgatggat ctggaagtat ctactatgga
     9061 accggaggaa gaagtgatcg tcgttgccgg aaatatcaaa caaacataag ttagaagagt
     9121 tactaaacga attacaacag atccccgaag aaatttgggg attctaccag tttcggcgag
     9181 atttattttg gaaaaagatt cctttgtcca agcagaaaat tcttatacaa caatcaatcg
     9241 attgtggtat cgaaacggca tgtagcatca aaaaaaagta cccttttgcc gatgtaggag
     9301 aaatctatga acaaatggct gttccaatcg tttcctgtga atcagagcaa ataaacgaac
     9361 gaatcacttt tgcaacttat gcagaagatg aagggatccg attgatgacg gagccgttag
     9421 aaaaattaaa atgttcagga ctagcttcga tctctaaaga aacagcgcaa gctttgatta
     9481 ttggacatga actatttcac cacatcgaag cttctgttaa gggaatttat acgcaaagcg
     9541 aaaaaattgt gctgtggcgg ttgccttttt atacacatca atcaaacatt cgtgcattga
     9601 gcgagatagc agcgatgagt ttttcaaaag agatgaatca aagcgatttt tcgccttatg
     9661 tattagaggc agtcttactt tggccatata atgaaattcg tagccaaggg atcttagaag
     9721 aaataaaaga aatagaaaaa agatgtacgg agtatgattt agattacaaa taaagagact
     9781 gtgacaaaag tcgtgtcaca gtcctttttt tccgaataaa cggtgttctc ctgtcaactc
     9841 ctcggtcaca agtcaaaata ttcaacaaac atgagaagct gttttttgaa attgttcctt
     9901 gtgactcgga gatgaacggc aggctcacga cctcttggaa aaaggtgttc aaaagcttgc
     9961 atctgcggta ataagaacag ccaagttaaa aacataagaa gctattttta cttggctgtt
    10021 cttattactt gatgcagggc agctttttca caacctctca ataaacggtg tgcaatcagc
    10081 tgaccctcgg tattagctca aaaaaaacgg caaaatatga ggaactattt tgccgtttat
    10141 ttctctaata ctcaggtcaa aacgctgatt ccaaaacctc ttatttgatg ggtctgtcct
    10201 gaatatcttc aaaataatcg gggtaattat gaatcacgta ttcttgctcc tcgataatca
    10261 tatgtaaatg catcgtgcaa agaaatccga cttcatcaaa gttatgatca atcattgatt
    10321 gaagcattgt ctgatgttca tctaatactc gaccccagtc taattctgaa atcgtcaaac
    10381 gtaaccatct gaaacggttc aagtgcgtac tataactttc tagccaatcc caaatttctc
    10441 gttttccagc aatttcataa caagttcggt gaaaatcagt atccaaatca aaaaaagctt
    10501 ttttattttg agcgatcact gcctgatcgg tttgttctag tattttttct agtttagctt
    10561 gatcttgctt ggtcattttc gcactgcatt cttgcaggat cggtttttcc aacttttccc
    10621 gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa gtgccggact
    10681 gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct cgaatcggtg
    10741 tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt ccaggtgcta
    10801 actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc aaattattga
    10861 taaccattta accttccttt ctatgcagat ataatctatt ataacataaa tcgcttacaa
    10921 atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg cctattagag
    10981 cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttggatcggt ttgacacgac
    11041 cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg agcagattgt
    11101 ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc attactttaa
    11161 cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact cgacacaata
    11221 caagaaaaat gcttagacgg tatttattaa gaatttacat catccaaaga agcgtacgct
    11281 tggaataact gttctaactc cggattgact ttgctcagct tcactaatcg ttgcgaatca
    11341 gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc ttcagttccc
    11401 acaatttcgt agctaaaatc caaccgtgtt cccgtatact tttggatagt tgggtggatg
    11461 ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc cagaacgggg
    11521 atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac caataaggct
    11581 acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc catttgatct
    11641 gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac tcatgcctcg
    11701 ttttccattg attttttaca aaaataattc atccacttcg gaagtgcttc gctgatttgt
    11761 gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact atgaagataa
    11821 acagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc tgtgatcatc
    11881 ccagctaaag tgtcacccat gccgcctgtt gccatagcag cagaacctaa aggattggtg
    11941 tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag cacagcaccg
    12001 atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg tagagaactc
    12061 aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata gggtagcaaa
    12121 agatgattag caacaagtaa acttaaagca gagccatcaa tcaccagcca ttgttctttt
    12181 gattgttttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt acccaatccg
    12241 ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc agtatccgtc
    12301 caatctacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt tttttcatca
    12361 gttactacag tcgtgagacc cgctccactg tgtacggcag ctaaagcact cataatgatt
    12421 gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt tcctttatgt
    12481 gaaatctttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat cagcatcgaa
    12541 aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga taagaaaagg
    12601 aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga acacgaagac
    12661 ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt agaagcacgt
    12721 aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga gcgcgatgat
    12781 tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt gaaacatatg
    12841 ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta tgcagggcat
    12901 attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga tgtcgttcct
    12961 gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg aaagatcttt
    13021 gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc gatgaagatc
    13081 attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg cagcgatgaa
    13141 gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc agattttggt
    13201 ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt ttctttagct
    13261 ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt ttccggactt
    13321 cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag tgcagatgca
    13381 gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt aagcggaacg
    13441 attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc acatggtgca
    13501 agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga taactatgaa
    13561 ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca tgaagatttt
    13621 tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt gacaatgaat
    13681 gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat caatatgaat
    13741 ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga acaaacagtg
    13801 gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca ttacgtacca
    13861 atggatgatc cattggtggc tacattattg caagtctatg aagaccacac tggagaaaaa
    13921 ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg tggcgttgct
    13981 tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga atttatgagt
    14041 cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg tttggcgaaa
    14101 taaaaatact aaaaaactcc ggctatctct gacagctgga gtttttatgt ttaaaaatga
    14161 attagtacat cggtattctt aaaagtaatt tagttcgtct tttgatttct caggagggat
    14221 caaaaaggcg aaagcactaa gtatagcaaa acttgtgatg atttgcgtac gatacgtaaa
    14281 ccaataatga atctgtgagt gattagctaa aatattgtac caaatatacg gcgttacagc
    14341 agctaaaaca aacaaaacta gaagttggaa cggacgccag ctatttcgtt tacgataagc
    14401 aaaatagaga aaaacaagac aagtaatcaa tagcaataat attgctactt ttggatacat
    14461 caatctaata ttgcttttca gcatctctaa tcgatgcaac gggtatttgt cgtttccttc
    14521 cgttcgaaag aggatttggt tgattgcatc ttttacaatg tttttgttca atatgatcga
    14581 tgataggatc catttagcca cccatgtcaa accatagcct aatccccaaa agataccggt
    14641 aaaaacaaaa ctgcctactt gtttgctcaa tgaactgcgt ttatccatta aatacttatt
    14701 tgttacataa taaataatga tcacaggtat cccccaagag atgaccggtg ttgtcaacaa
    14761 gtcaaaaaag ttagctaaag aaccaatgac aaagaaatac aagaataatt gttggttggt
    14821 gttggagagt tgtggttttg ttaaaaatag taatacagac aataaagtca aaatccaaat
    14881 atttgaaaat tgcatactga agaaaaaggt tgcagcattt cctatagcaa gcgaaatcaa
    14941 caaggctgtc gttaaataga tgtcgctaca cttggttaaa aagtagctcg tcaatccaat
    15001 cagcagaaaa agaatggctg cgtaaatcat tcgaatactc cccaaactca tccctagcag
    15061 taaaggtcgt aaaaaaagct gataaccatg ccaataacgg ggataatccg ccatagacat
    15121 cgcgttctcc aaggagttca attcaggatc tgcttttgtt tttcgtatca tcaaatgatc
    15181 agtaaaatta tctaaccgag tgcctagcat atttccttga ttgatagaag gatacattcc
    15241 ttctttttca accatctgta gagatttttc tgcattcgat tcaatagagt gctgcggaat
    15301 gaaaaaagta attgtcagta gtacgcaata gctagcgatc aagagaacaa aagcaaaaaa
    15361 tatcttttta aatagtttca tgagtatctc ctatatctgt tttcattttt tctcttttat
    15421 ctagtaaaaa aggagagagg atataaacag gtagacctaa tgctaaaaca aacacatacc
    15481 gtaaactaaa agccacagga gtagcaatca taatagtagc aaaaacaagc ataccgggaa
    15541 gaaaaaggag taaataccga tattttttct taaagattgc taaaagtgca ctgccgatag
    15601 ctaaccacag taatgtgccg gaaccaaaga aatcacgagc atcgatgatt tttttcattc
    15661 ctgacaatcc agtaagatgt tcaatcaaat ctacttgctt cataccataa ttattcgtgt
    15721 ttacacgagt atctaaaaag ccgtaactat tttttgtacc aggtacccag aacccgaacg
    15781 tttctaaaat ataagcatct gtatagattt ttatattact tggtaaattt cttgcccata
    15841 gtgatagaaa ttctttttta tgtgtattca aaaactcatt gttaaaattt ttatgccact
    15901 tgattgagtc tgcaagacta ggagaatacg cttcataatc ttttatcggg agtagctgat
    15961 tcatgaatgc tttatctttc ttagacaagt caccatccat aacaatagtt ctagaaattt
    16021 gctgcaaagg aacacctaat tgttcagcag tttctgtagg aatagcataa cgagccatca
    16081 acgggtggat gagtagtcca gcaatcactg tataacccag caccacttga aagaatgctt
    16141 gatacctttt ttgtttcaaa ataatcaagc cgcaaatcaa acctgtgccg gttactacat
    16201 aaatcccatt atttcttaga taggtggtca gtaaagcaag gatgattacg taaatctgat
    16261 tgagaatcgt tggcttttct cgttgaaaaa taaagtcaaa aaccgctaag gaaaataaaa
    16321 agcaagcgat agagaacaaa gtatcttttt gcatgttcaa ggcgtagatt ggaaaaaccg
    16381 gtgcaaatgc aaaatagcat aaactaatga gtaccggaaa aaaatggatg tttcttttta
    16441 acagccagca aagagaataa cttatcgctg aagctgttat caatgtttga aaaatcgaaa
    16501 ataaaaatac accgtgattg atcgtatcag ttggtaaatc atgaacaaat agcttgacca
    16561 gtaaggtgaa taagactgga tgatgattgc ttataggaac atcacccata gattgggcga
    16621 ccgaacttaa cgaatcaggc aaaaccactc caggatacaa tgccaacaga taaggaagcc
    16681 aacacagaac taaaaagaca gtaaagaata aaaaatattt tcttgaagag aaagcctgtg
    16741 tgttttgagc agtaaaaaat gtttccagtt ttggcattga taaataaatc gattgtaaaa
    16801 ataaaaaaga atacacaaaa atggagaaat aaacaagaaa gtaaaaaata cttccatcag
    16861 caatcgttgt ctttgtatag tttgctgaga catactgaaa ttgtatctgt ctgccaatag
    16921 aaagtgtaag agcaaaagct gcagaaaaaa acaatcgggc aatagctgca actttgtttt
    16981 ttattgaaaa caactctatt ccatattgct taaaagcaag tagcaaaacc atggctaaac
    17041 caatgattac tgttggattc aaagttccta gaccataaga agtaaaaaaa gcaaaacctg
    17101 acgcaaataa agttgcaaaa aaagtaggaa atagattctc ctttttctta tgcataaaaa
    17161 aggcctccta cttcatttct ttattttgat attcgtggta gacatggtat aaattcaatt
    17221 catattcttt cttggcattt gtgacgaccg tatccaaaat caaccctgtt actaatgaaa
    17281 gaagggctaa agtcatcaat ccagtactta aaatcgcaga agggatgcgt gtaataaagc
    17341 ttgtcagcat atattcacga atcacaggaa tccccatgac caatccaaat aaaaagaaga
    17401 agatggtcca gatcccaaag aaaagcaaag gtttataatc tttgcacatt ttgatgatca
    17461 tcatgatcac tttgaatccg tcagagaatg tattcaattt tgattcgcta ccttcaggac
    17521 gatcgcgata atcgattgga atctcaacga acttgaattt tttatctaaa gcatgaatcg
    17581 tcaattctgt ttcgatttga aaaccagaac tcataatagg gaagcttttg acaaaaattc
    17641 gattgaatcc tcgatagccg gtcatgacat cgcgaatttt tgttttatat aagtaagtaa
    17701 ttgtattttt aaccagatta ttcccaaaat catggaaagg acgcttattt tcatcaaaat
    17761 aggttccatt tgatagacga tcgccaatga ccatatctgc ttcgccagaa cgtaatttct
    17821 caagcaaacc atgtgcagct tctgcaggat aagtatcatc accatctacc atcaaataat
    17881 aatcggcatc aatatcaaaa aacatttgac ggattacatt cccttttcct tgacgtggtt
    17941 ctttctttac gattgctccg ccttgaacgg ccaattcgta tgttcgatcg gtcgaattat
    18001 tatcatagac ataaatatct gcttctggta attccctttt aaaatcttca ataacagttg
    18061 aaattgtcgc ctcttcgtta taacaaggaa tgagcacggc aattttttca cgtgattcca
    18121 tttgtcactc tccttatata ataaaactaa tcttatttca ataataccgt ataataacta
    18181 caaatactag ctattccaag aggaaatagc cctattttta taaaaatcac ccactataaa
    18241 agaaggatag gatacctctc ttttagatag tgggtgcgta ttcgttattt catttttttg
    18301 acgtattaat ctattgcgcc cttttttctt gaaatgtcta atacggttcc tgtatgagca
    18361 tctgctaaaa attcataata aacagtttcg ccatcttcta tccgggaaag cccaccccga
    18421 taagcactcg accgaacggc gaatttccga ataggttctt tttcaaaaga gatccaagca
    18481 ccttcgattg cgccttcttt taaaaaagat gttttgatat tttcaagcac ttggtctgct
    18541 gataacactt ttttgcgatg ataccaaatc gttgaagcta ccccactcaa taatcctaga
    18601 ccgatcccaa cggctaaccc gcctttaaaa tagcttaatt cgctttcttc atacatgtgg
    18661 atatacctcc ttcttctcac ttgctactct ttgtgtttat tttaccacac tctcatattt
    18721 tctttgaaat aaataaccgc taccagaaaa aataagctat aataaatacg atcggtctgc
    18781 agaaaaaatc atattttatc cgcagatcaa tataagtctg agaggagttt agtctgaaat
    18841 ttagacgaat agcatatgga agaaaaaaca tttcaacgaa tcaaagagtt aacagaatta
    18901 caaggaacaa gcggttttga acaagacgtt cgcgcctata tggaaagtca tatggaaccg
    18961 ctggtagatg agttacagtt agatggtttg ggcggcattt ttgggctgcg ccaccatgaa
    19021 gaagcggatg cacctcgtgt aatggtcgca gcacatatgg atgaagtcgg attcatgctg
    19081 acacaaatcc aagaaaacgg gctttttaaa gtcgtacctc taggcggatg gaacccttac
    19141 gttgtttctg ctcaacgttt tacacttaaa acctctactg gtaagaatta tccatgtatt
    19201 tcttcttccg ttcccccaca tttattacga ggaacaagcg gacaaaaatc agtagaagta
    19261 acagatattt tgttcgatgc tgggtttgaa tcaagagaag aggcaatgag ttacggtgtt
    19321 cttccaggag acacaatcgt tccttacgct gaaacgatca aaacagccaa cgggaagaat
    19381 atcatcagca aatcgtggga caaccgctat ggctgtacaa tggtattaga agctttagaa
    19441 gcgttacaaa atgaaacatt aggtcataca ttgattgcag gcgcgaatgt ccaagaagaa
    19501 gtaggcctgc gtggttctaa agcttctgtc aataaattca agccagatct gttctttgca
    19561 gtagactgct ctgcagcaga tgacacagtg acgaaaaatg gcacattcgg acatttagga
    19621 gaaggtacat tgatgcgtat ccaagatcca ggtttgatca tgcttcctcg tttgcgtgag
    19681 tacttgctag atatcgctga aacaaacaac atcccttatc aatactttgt ttcaaaaggc
    19741 ggtacagatg caggtgctgc tcatacacaa aatgaaggaa tcccaagtac agtgattggc
    19801 gtagtgggac gttatatcca tacgcatcaa acgatgttca gtattcgcga ttttgaagct
    19861 gcacgagaaa tgttgattca aacattgaaa ggtttagata aatcaacagt gaacacgatc
    19921 gtatacggga aataacgcga tagtgaaact ataaaatctg gcaaaagcaa ccttttgtca
    19981 gatttttttt ataaaaagag gatcaatgtt tgttagttcg aaagaaaggt gtataattta
    20041 attgaaaaat acataaacag aaagtagagg aaaaagaatg attatcccaa atagtttaga
    20101 agaattagca ggctacgtag aaactggaaa aagtgtattt ttctttactg ccggctggtg
    20161 cggagactgc cgttttatca aaccgcaaat gcctgaaatc gaaaatgact tttctgattg
    20221 gcaatttatt gaagtagacc gtgacaagta catagatgta gccgctgaat ggaacatttt
    20281 tggtatccca agttttgtcg tgatccaaga cggaaaagaa ttaggtcgtc tagtgaataa
    20341 agatcgtaaa acaaaacagg aaatcgaatc gttcctagaa agtgtaataa aaggctgaca
    20401 tctgttagga ggggtcaaga tgaatcaaga atataagaac attttagtag gtattgacgg
    20461 tagtgaacaa gcacttgctg ctttcaaaaa agcgttagaa gtggctcgcc gtaataatgg
    20521 gaaagtatat gtagcaaacg taatcgatca gcaattttat aattttatgg gctatgctcc
    20581 tattgatcaa aatctagtcg atcagcaaac tgaaacagcc aagcaaatga tcgaagaatg
    20641 taaagaatat ggaaaatctg ttgattacac aaatatagaa ggaattattg catacggctc
    20701 tgttcgtgaa tctatggcag tcaaattgcc aaaaaaatat gcaatcgatc taatcatggt
    20761 tgggcagtct ggactaaatg cagtcgaacg ctttatgaca ggaagtgtag ccagctacgt
    20821 catacgtcat tcaccttgtg atgtcttgat cgtttctgac gaagaagaac agccgagtaa
    20881 ttaagcaagt ggaacttgta taaccttttt aatcaaacag taaagggact gtgacaaaag
    20941 tcttgtcaca gtcccttgtt acgaataaat ggtgttccag aagaagcttc ttcggaaata
    21001 aagcatgaaa aaaatctcat gcagtgaatg caattttttg ttaagataaa tctcagcaaa
    21061 ggataatgga ggaaagaaca tgatttttgc ttataacaaa gcacacgtcg gagacacttt
    21121 gatggttatc atagccgacg ataaaggaac agaaaacact gtcgaacgta aatggaatgt
    21181 tgctcggatc aaagatgaac aaggtcaagt tgttgcttgg aactttttcc atatctccga
    21241 tcatctaaca attgaaggga acggccaagt aagcttaaca gatgaagaat tagcgaaatt
    21301 gaatcgattg attaaagaag caggattcga agaacagctt gaagctgatc atgaaccgaa
    21361 gatcgttgtc gggtatgtaa aaacatgcgt cccgcatcct gactctgacc atttatcgat
    21421 tactgagaca gaagtggaca atgggacagt attgcaaatt gtttgcggtg caccaaatat
    21481 cgaagctgga caaaaagttg tcgttgcaaa accaggagcc atgatgccag atggaatgat
    21541 gatctggcca ggaactttgc gaggggtagc cagctatgga atgatttgtt ctgctcgtga
    21601 attgcatttg ccaaatgctc cagaaaaaaa aggaatctta gtattgcctg aggatgccgt
    21661 agtcggagaa gtatttccaa aataaagagg ttgtgacaag tctttgtcac aacctctttt
    21721 ttattaattt tcactatttt gattttgttt taatgccgat tgatcgatcg tcaattcaac
    21781 agtagttgtt ttttctttcg agccttcata gtaagtgatt ttgacagaat ctcccacttt
    21841 tttcttatac aaagcagatt gaagttctac accagaagaa acttctttgt catcgatctt
    21901 agtaatgacg tcgtattgtt tcaacccagc tttttctgcc ggtgtagctg tttggacatt
    21961 tgtcacgatg acaccgttag ttacagatga aggaatcttc aagatttgtt cttgttgctg
    22021 agtagaaacg gcagataagt cgaccattgt gatacctaga gctggacgtg tcacttttcc
    22081 gtctttttcc agttggttga tgatattcac tacatcgtta cttgggatgg caaatcccat
    22141 tccttctacg cttacattag aagtcgattc agaagtactt gcgatcttac ttgagttgat
    22201 accgataact tgtccttcga tattcactaa aggaccacca gaatttcccg ggttgatcgc
    22261 tgcatctgtt tggatcgcat tgatattgac tgtttcgttc gattcattcg tacttgttac
    22321 ttgtcggttc aaggaagaaa tgattcctga agtgacagaa ttcgcatatt cagatcctaa
    22381 tggggaacca atcgcaatcg caggttcacc aaccttcaaa gcacttgagt ccccaaaaga
    22441 ggcaaccgtt tcgactttgt ctgaattgat cttcaacacc gctaaatctg aataggcatc
    22501 tgttccaact aattcagctt ttaccttcgt accgtctttc atcatgactt ccaaaccttg
    22561 ctgtccatct actacgtgat tgtttgtgac tacataagca gtattcccgt cttttttgta
    22621 gattacgccg cttccttcac tataagcttc caaattacta tcatctgagc tgctactttc
    22681 ttcttgctgc ccgaataatc cgccaaaacc gcttgattgg ttttgacttt gtaagttgat
    22741 aacagaaacg acagcgtctt gaactttatc taccgctttg gtgatatcag aatctacatt
    22801 gactttgaca ttttccacaa ctgtttcacc ggcagaattt tgattgccgc ttgtagcagt
    22861 agaactattg ttgccagtgc ccatcgctgc ataaaaaatg ccagctgtca gtagtcctcc
    22921 aacgatccct ccaacaagcc ccagacccaa cttacgccag aggctattat ttttattttt
    22981 tttcatcttt ggtgtcacat tttttctatc catgtgttcc tcctccaatt acattcattt
    23041 atctactgtt agtatagtct tatttgttga ctttagtcta ctttgagact ttgaactttt
    23101 tatgaaaaac tttaggaaaa atcattgttt tcagatgaaa atatttgatt ttctttttcc
    23161 acagatattg tgtaaaacta cgcggatagg attggaaaaa taagggataa aacatatcca
    23221 cagcctgtgt ataaagtgga taaattctta taaaacgtat gtttgcctaa tgaatgtgat
    23281 gaaataaggt aaaacaacgc tgttttctgt ggataaacct gtggatgttg tgcataactg
    23341 ttggggaaat ctttttaaca aaaatagtca aaataaacat atagacgaaa aaattgatta
    23401 taataaacaa agtgagcgtg aagatcaaag gagagaaaac atgaatatca aaattatttc
    23461 agtaggaaaa ttaaaagaaa aatacttgat ccaaggtatc aatgagtatg tcaaacggct
    23521 gaatgcctat gcgaaaatcg aattgatcga agttcctgac gaaaaagccc cggagaattt
    23581 aagcgaagcg caaatgagac aagtaaaaga aaaagaagga gaacggatct tagcgaaaat
    23641 caaagaacaa gaatacgtct atggcttagc aattgaaggg aaaaatccca caagcgaatc
    23701 ttttgctaaa caaatcgacc aattaggtat ccaaggaaaa agccaccttg tttttgttat
    23761 cggcggttct ttaggactca gcgaagccgt catgaaaaga agcaatgctc agatctcttt
    23821 tggcaaaatg acgtatccac atcaattgat gcgcctgatc ctcgtcgagc aaatctaccg
    23881 tgccttccgg atcaacgcgg gggctccata ccacaaataa tgatattttt taagagaaaa
    23941 atagcataaa aatctagtta tccgcataaa aactggactt atcacacttt atcaaggtca
    24001 aaaccactca atttactact aatttactac ttatgaatga gctttgatac gacgatttat
    24061 ccttgaaaag tgaagatata aagatacttc caataaaatt tgaatattta ataggtagac
    24121 acttcaaaaa atgaggtgtc tattttttta cccgattttg aaaggaagtg aacttatgaa
    24181 aacaaaaaat caagaatcaa aaggtcgttc cccactcttt aagaccatca aacattcatt
    24241 cagccaataa aaaagaaagg ataggtaaaa atatggaact taaatttgtg attcccaaca
    24301 tggaaaaaac attcggcaat ttagaatttg ctggcgagga taaagtcgtt cagcgaagaa
    24361 tcaacggacg gctaactgtc ttatcaagaa gctataatct ctattctgat gttcaaagag
    24421 cagatgatat tgtggtggtg cttcctgctg aagctggcga aaaacatttc ggctttgagg
    24481 aacgtgtgaa gttagtcaat ccacgtatta ccgcagaggg ctacaaaatc ggcactcgtg
    24541 gttttacaaa ttacctttta catgctgacg acatgataaa agaataaaga aagagaggaa
    24601 aaatgatgag attagcaaat ggcattgtat tagataaaga cacgactttt ggagaattga
    24661 aattctctgc tctacgtcgt gaagtgagaa tccaaaatga agacgggtcg gtttcagatg
    24721 aaatcaagga acgtacctat gacttaaaat ccaaaggaca aggacgcatg attcaagtaa
    24781 gtattcctgc cagcgtgcct ttgaaagagt ttgattataa cgcacgggtg gaacttatca
    24841 atcccattgc ggacaccgtt gctactgcca cctatcaagg agcagatgtt gactggtata
    24901 tcaaggcaga cgatattgtg ctgacaaagg attctagttc attcaaagct caaccacaag
    24961 caaagaaaga accgacacaa gacaaatagt cgctaggtag aaaggagact ttttcgcatg
    25021 aaacagcgtg gtaaaaggat tcgcccatct ggtaaagatt tagtctttca ttttacgata
    25081 gcgtcactcc tgcctgtttt cctgctggtt gtcggactgt ttcatgtgaa gacaatccag
    25141 cagatcaact ggcaggattt taacctatca caagcagata agattgacat tccctattta
    25201 attatcagtt tcagtgtcgc aattcttatc tgcttgctgg tagcgtttgt attcaaacgg
    25261 gttcgctatg atacggttaa acaactttac caccgtcaaa aactggcaaa gatgatactt
    25321 gaaaacaagt ggtatgaatc tgaacaggtc aaaacagagg gtttctttaa agatagtgct
    25381 ggtcgtacaa aggaaaagat aacctacttc cctaaaatgt attatcgact taaaaatggc
    25441 ttgatacaga tacgggtgga aatcacgctg ggaaaatatc aagaccaact cttacacttg
    25501 gaaaagaaat tagagagtgg cttgtactgt gagctgacgg ataaagagtt aaaggattcc
    25561 tatgtggaat atactttgct ctatgacacc atagccagtc gtatttctat tgatgaagta
    25621 gaagctaaag atggtaaact tcgcttaatg aaaaacgtat ggtgggaata tgataagctc
    25681 cctcatatgt tgattgctgg tggtacaggt ggcggtaaaa cttactttat actgacactg
    25741 attgaagcct tgcttcatac agattcaaaa ctgtatattc ttgacccgaa aaatgctgac
    25801 cttgcggact taggttctgt gatggcaaat gtctactata gaaaagaaga cttgctttct
    25861 tgcattgaaa cattctatga agaaatgatg aaacgtagtg aggaaatgaa gcagatgaag
    25921 aactataaga ctggcaaaaa ttatgcttac ttaggtctcc cggcacactt cttaatcttt
    25981 gatgaatacg tcgctttcat ggaaatgctg ggaacaaaag aaaacaccgc agttatgaat
    26041 aagctgaaac agattgtcat gttaggtcgt caagctggct tctttctaat actggcttgt
    26101 caacgtccag acgcaaaata tttaggcgac ggaatccgtg atcagtttaa tttcagagtg
    26161 gctttaggtc gtatgtctga aatgggctat ggcatgatgt ttggcagtga cgtacaaaag
    26221 gatttcttct taaagcgaat caaaggtcgt ggctatgttg atgtaggaac aagtgtcata
    26281 tcagagtttt atactcccct tgtaccaaaa ggatatgatt tcttggagga aattaaaaag
    26341 ttatccaaca gcagacagtc cacgcaggcg acgtgcgaag cggaagtcgc aggtgtggac
    26401 tgatcttgct ggctggtgtg gcaatagcca cgccagcact taacccctcg gtatctaaca
    26461 ggggggtaca aatcgacagg aaacagtcaa aaaaacatta gaaaatcctt tggttacaag
    26521 ggatttacaa aatttcagcg tatgtcaaat gggctttaaa agttgacata cgcctttttg
    26581 attggaggga tttttactga atgaacaaac ttggttacag catttaaaag aaaaacgctt
    26641 ggcttatgga ctatctcaaa accgtttagc tgttgcgact ggtattacaa ggcagtatct
    26701 aagcgatatt gaaacaggaa aagtcaagcc atcagaggat ttacagcagt ccctttggga
    26761 agctctggaa cgcttcaatc ccgacgctcc ccttgaaatg ctgtttgatt atgtaagaat
    26821 tcgctttccg acaacagacg tacagcaggt ggtcgaaaac atcttacaac tgaaactgtc
    26881 ctattttctt catgaggact atggtttcta ttcttattca gagcattatg ctttaggcga
    26941 catattcgtc ctttgctccc atgaactgga caaaggagtt ctggtggaat tgaaaggtcg
    27001 tgggtgcaga caatttgaaa gctatcttct ggcacaacaa agaagctggt atgagttctt
    27061 tatggacgtt ttggtggctg gcggtgtgat gaaacgcctt gaccttgcca ttaacgataa
    27121 gacagggatt ttaaatatcc ctgtactcac tgaaaagtgc caacaggaag aatgtatctc
    27181 cgtcttccgc agttttaaaa gctatcgcag tggcgaactg gtacgcaaag aggaaaagga
    27241 atgtatggga aacaccctct atatcggttc attacaaagt gaagtttatt tctgtatcta
    27301 tgaaaaggac tacgagcagt acaagaaaaa tgatattccc attgaagacg cagaagtaaa
    27361 aaaccgtttt gagattcgat tgaaaaatga gcgtgcctat tatgcagtcc gtgatttact
    27421 cgtctatgac aatccagagc ataccgcctt taaaattatc aatcggtata tccgttttgt
    27481 agataaagac gattccaaac ctcgttctga ttggaaactg aatgaagaat gggcttggtt
    27541 tattgggaac aatcgtgaac gattaaaact aaccacaaaa ccagagcctt actccttcca
    27601 aaggacgctg aactggctat ctcatcaagt tgccccgacc ttaaaggttg cgattaaact
    27661 tgatgaaatc aaccagacgc aggttgtaaa agacattctc gaccatgcga aactgacaga
    27721 ccgacacaag cagattttga agcaacagtc agtaaaagaa caggacgtga taacaacaaa
    27781 aaaataactc aaatacaaat tcattgaata tagagaggag aacattttta tgaattttgg
    27841 acaaaacctt tgtgcgactc gttccttagt gaaaagctaa ggcactaata aaagaacgaa
    27901 aaagtttggt tattaggaga actgataatc tgacaggtgg aatgatgaag taacgctctg
    27961 aaacgcctgc cctgagcctc cgacaagcat tgtatgagga aactcagaat gtttgaagct
    28021 cggtaaagac ggctgaaaga taccgtaata gttgatgaac atatatcttc tatcgaaaac
    28081 tatccagagg tgggtagcgt atccaacagg tcgggggtct ataaaatatc tatggttaga
    28141 atgttctata aggaactgac gaaacagcga atgtacgggt ctaaatttga actttgtaga
    28201 aatgcaaagg tacgagaaat cgtagctggt gtggcaaagt aagaatatag gttatgaaat
    28261 gccatataac gttacaggcg gtatcaagcc agcaggctta tagctagtga cctaaggata
    28321 tatatgtata gataagatta tcggaacgag gaaaggtatt gggttctcat caagagaata
    28381 aactgacgaa gaacaataag cagttgaact aatgctgaaa agcagagggc gaacctaaga
    28441 aattcttgta atgagaatgg aggaatgccc tcaagtcgtg tatatattag aaaagtatta
    28501 tgttgtttca ataaggatta cgagtaagat aaaaaagctc actcaatttc aagtaaagga
    28561 gtgaagcctt atgcctaaaa ataaaaatga aacattgtgt gtagaagatt taagacacgc
    28621 tgaatattac gaaatgcaaa acacctttga tgatttatat gccaaaagta agaatggaga
    28681 tattttcact catcttatgg atattatcct atcgagagaa aatatattac tggcatatcg
    28741 aaatatcaaa gcaaatgcag gcagtaaaac agcaggaaca gacggtacaa taattaaaga
    28801 tattggcaaa ttgccagcag aaacagttgt caaaaaagta agatacattg ttgcaggcag
    28861 tccacatggg tatcgaccta agccagtgag gcgaaaagaa ataccaaaac caaatggtaa
    28921 aaccagacct ctaggtattc cttgtatgtg ggacaggctt atacaacaat gtataaaaca
    28981 agtattagaa ccaatctgcg aagctaaatt tagtgaaaac agctatggtt ttagacctaa
    29041 taggtcagtt gaaaatgcaa taaaagcaac ctataaccgc ctacaaatca gccagctaca
    29101 ttatgtgatt gaatttgata ttaagggttt ctttgacaac gtgaaccatt ccaagctgat
    29161 aaaacagata tgggctatgg gaatacggga caagcacttg atattcattt taaaaaggat
    29221 attaaaagct ccaatcaaaa tgactaacgg tacaataaca tatccagaga aaggaactcc
    29281 gcaaggggga ataatatccc cactactagc aaacattgtt cttaatgagt tagaccattg
    29341 ggtagaaagc caatggcagg aaaatcctgt tacaaaaaac tatgtggttc atatcaataa
    29401 aagcggtagc ccatgtaaaa gcaacgctta caaagaaatg aagaaaacaa agttaaagga
    29461 aatgtatatg gttcgttatg cagatgattt tagagttttc tgccgctaca aggaaagtgc
    29521 tgaaaaagcc aaaatagcaa tcactcaatg gattgaacaa cgattaaaat tggaagtatc
    29581 acaagaaaaa acaagaattg taaatgttag aaaaagatac tccgactttc ttggtttcaa
    29641 gattaaaatg ataccgagaa gaaagaagtt agttgttaaa tcacatataa gtgataaaca
    29701 attcaaaaac caaaaagaca agctggtatc acaggcaaag aaaattgctt ctccctcaaa
    29761 agggaaaacg gaattagaag aaacaagact ttataattca atggtattag gtatgcaaaa
    29821 ttattatcag attgcaacga atgtaaatct ggattgtatg aaactgaata gagctgtaat
    29881 ggctatcttc actaacagat taggtacaaa caaaaggggc agattgagaa aaacaggcag
    29941 acaacttacg acaaaggagg caacaagata tggtaaatct aaaatgcttc ggtatgttgc
    30001 tggcgagggc gaacctattt tccctgttgg atatatccaa cacagaaaac caatggcaaa
    30061 ggtgtattta gcgaattgct acactctaga gggaagaaaa tttatccaca ccaatctaag
    30121 tgttgataaa ttactgatgt accagttaat gaaattatct ttggaaaatc gcacaataga
    30181 atacgcagac aatcgaattt cgctattttc tgctcaacat ggaaaatgtg ctataactct
    30241 ggaagaattt caacaagcta atgaaataca ttgtcatcat attgtaccaa ctggagctgg
    30301 tggaaatgac gattataaaa atcttatttt ggtaaaagag gcggtacacc gtctaataca
    30361 cgctaaaaca gaagaaacaa tacaaaaata tatagctcta ttgaagcgaa ataaagtcca
    30421 gctcataaga ctaaataaat atagagtatt agctggcaat caagaattaa acttgatata
    30481 ggtctactac ataaatactt ttcaatataa acacgatgga acgcggagtg cggtgaaagt
    30541 cgcacgctcc gtgtgaatgg ggggaaaagt ttgagatatt tataatatca ggaacttacc
    30601 tatccatata taactggttt ctatcaaacg ctcaatcact ggtgctttta gcaatcgttg
    30661 tgattggctt gtatcttggc ttcaagcgtg agtttagcaa actgattggc tttttaatta
    30721 ttgcgattat tgcggttggc ttagtcttca acgctgctgg agtaaaagac attttactag
    30781 agctattcaa tcgcattatt ggtgcttaaa taaaaccgtt cttttgtgga atataagtgg
    30841 ttttcttatg ttccgcaaag gaatggtaca ccaaacgaag tgcggtaggg atttttgaat
    30901 ctctacaaag aaaggacgtg aatatatgga cgatatgcaa gtctatattg cgaatttagg
    30961 caaatacaat gagggcgaat tggtcggtgc gtggtttacc tttcccattg actttgagga
    31021 agtcaaagag aaaatcggct tgaatgatga atatgaggaa tacgccattc atgattacga
    31081 gttacccttt acggttgacg aatacacttc tattagcgaa ctcaatcgcc tatgggaaat
    31141 ggtatcggag ttgccagaag aactacaatc ggagctatct gctctgctca ctcatttttc
    31201 aagtattgaa gaactaagcg aacatcaaga agatattatc attcattccg attgtgatga
    31261 tatgtctgac gtggcacgct actacattga agaaacgggt gctttaggcg aagtgccagc
    31321 cagtcttcaa aactatattg attatgaatc ctatggtcgg gatttagacc tttcgggaac
    31381 gtttatctca accaatcacg ggatttttga aatcgtctat taaatctgtc ggtacattct
    31441 tactggcaga ttttcttgtt tacggggtag tcaaaagact atccccattt ttatgaaagg
    31501 attgattaca tgaagaaaat acgaagctat accagtatct ggtctgttga gaaagtgttg
    31561 tactccatca atgactttag acttccgttt cccataacct ttacgcaaat gacatggttt
    31621 gtcgtgtcac tctttgcagt tatgatactt ggcaacttgc cccctctttc tatgatagag
    31681 ggagcatttc tcaaatactt tgggattcct gtggctttca catggtttat gtctacaaaa
    31741 acctttgatg gtaaaaagcc ttatggattt ttgaagtctg tcattgctta tgcactgcga
    31801 cgaaagctga cctatgcagg aaaaaaagta acgcttggca gaaaccagcc acaagaagcc
    31861 attacagcag ttaggagtga attttatggc atatccaatt aaatatattg aaaacaatct
    31921 ggtctggaat aaagatgggg aatgttacgc ttactatgag cttgttccct acaattactc
    31981 atttctaagt ccggaacaga aaatacaagt acatgattct ttcagacagc ttatcgcaca
    32041 aaatcgtgat ggcaagattc atgctttaca aatcagtaca gaatccagca tactttctgc
    32101 acaagaacgt tccaaaaatg aagtcactgg aaagctcaaa acggttgcct atgacaaaat
    32161 cgaccaacag acagacgctt taatatccat gattggcgaa aatcaagtgg actaccgttt
    32221 ctttatcggt tttaagttgc ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt
    32281 tgaagcaaaa aatgctttta ctgattttgt ctatgatgtg aaccataagc tgatgggcga
    32341 ttttgttagt atgagtaatg atgaaatcct gcgttttcag aagatggaaa agcttttaga
    32401 aaataaaatc tcccgtcgtt tcaaaatccg aagattagat aaggatgact tcggctatct
    32461 gattgaacac ctttacggac agacaggcac tgcctatgaa gagtatgagt atcctctatc
    32521 aaagaaaaag ctggaacatg aaacactgat taaaacctat gacctcatta agcctactcg
    32581 ctgtctggtg gaagaaaaac agcgatattt gaaaatccag caggaagacg aaaccgccta
    32641 tgtagcttac tttaccatca acagtattgt cggcgaactg gacttcccgt cctctgaaat
    32701 cttctactac cagcaacagc aatttacctt tccgattgat acgtcaatga atgtggaaat
    32761 tgtagcgaat cgtaaagccc tatctactgt ccgcaataaa aagaaagaac tgaaagactt
    32821 ggataaccac gcttggcaaa gtgataatga aaccagctct aatgtggcgg aagctctgga
    32881 aagtgtgaac gagctggaaa ccaatttaga ccaaagcaag gaatctatgt ataagctgtc
    32941 ctatgttgta agggtatcag caaatgacct tgacgaactt aaacgtcgtt gtaatgaagt
    33001 gaaagatttc tatgatgatt tgagcgtgaa actggtacga ccttttgggg atatgctggg
    33061 cttacatgaa gaatttttac ctgccagcaa aagatatatg aatgactata ttcaatacgt
    33121 gacctctgat ttcctcgctg gtttaggttt tggtgctact caaatgctgg gtgaaaatga
    33181 ggggatttat gttggctaca gcttagatac tggacgcaat gtctatctga aacctgctct
    33241 tgccagtcaa ggggttaagg gttcagtaac caatgcgtta gcgtctgcct ttgtcggttc
    33301 gctgggtggt ggtaaatcct ttgcgaataa ccttatcgtc tattatgcag tgctttatgg
    33361 ggcacaagca gtgattgtag acccaaaagc agaacgtggc agatggaaag aaaccttgcc
    33421 agagatttcc catgaaatca atatcgtcaa tctgacttct gatgagaaaa acaaaggctt
    33481 acttgaccct tatgtaatta tgaaaaatac caaagattct gaatcactgg ctattgatat
    33541 tttgacattc cttacgggga tttcctctcg tgatggggaa cgcttcccaa tccttagaaa
    33601 agccattcgt gcagtaacca atagtgaagt gcgagggttg atgaaagtga ttgaagaatt
    33661 acgggttgag aatacgccac taagtaccag catagccgac catatcgaaa gttttacaga
    33721 ctatgacttt gcccatctgc tttttagtga tggttatgta gagcagtcta tcagccttga
    33781 aaaacaactg aacattatac aggttgccga cttggtactt cctgacaagg aaacttcctt
    33841 tgaggaatat accacaatgg agttactatc ggtagcaatg ctgattgtca ttagtacctt
    33901 tgcgttggac tttatccata cagaccgaag tattttcaag attgtagact tagacgaagc
    33961 atggagcttc ttacaggtgg cacaagggaa aacactatct atgaagctgg ttcgtgctgg
    34021 tcgtgctatg aacgctgggg tatatttcgt gacccaaaat acagatgacc tcttagatga
    34081 aaaactgaaa aataacctcg gcttaaaatt tgcctttcgt tccactgacc ttaacgagat
    34141 taaaaaaacc cttgcctttt ttggtgtaga cccagaggac gaaaacaatc agaagcgatt
    34201 gcgtgatttg gaaaacgggc aatgccttat cagtgattta tatggtcgtg tcggtgtgat
    34261 acagttccac cctgtatttg aagaactgct ccatgccttt gataccagac cacctgtgcg
    34321 aaaagaggtg taaatgtgaa accatcaata ttaaacagaa taaaatcaaa ctggacgctg
    34381 aaacgtctag gtaaagtggc aatgacagtg gctttcacac ttgtgattgc catttttctt
    34441 ttagccatgc tgggaacggt ggttcaagct gcgggcttgg tagatgatac ggtcaatgtg
    34501 gcaaatgaat acagccgata cccacttgaa aactatcaac tggattttta tgtggataat
    34561 agctggggct ggctaccgtg gaactggtcg gacggaattg gaaaacaagt catgtatgga
    34621 ctatatgcca ttaccaattt tatttggaca atcagtcttt atgtttccaa tgcgacgggt
    34681 tacttagtac aggaagccta ttccttagac ttcatttccg ctacagcaga ttccattggc
    34741 aagaatatgc agacccttgc aggggttacg cctagtggac tatcaacaga gggtttctat
    34801 gttggattcc ttttactctt gattttggtt cttggggttt atgttgccta tacaggactg
    34861 ataaagagag aaaccacaaa ggcaattcat gccctcatga attttgtgct ggtttttatc
    34921 ctatcggctt cctttattgc ctacgctccc gactacatta aaaaaatcaa tgacttttca
    34981 tcagacatca gcaacgctag tttgtcactg ggtacaaaaa ttgtcatgcc ccattcagat
    35041 agtcaaggca aggacagtgt ggacttgata cgtgacagcc tattttccat acaggttcag
    35101 caaccgtggc tactgcttca atataacagt tcagacattg aaagtattgg tattgaccgt
    35161 gtggaaagcc tgctctcaac cagtccagat tccaacaatg gggaagacag agaaaaaatt
    35221 gttgcggaag aaattgaaga tagaagcaat accaatatga cgattacaaa gacgattaac
    35281 cgtttaggta cagtcttctt cctatttgtc ttcaatattg ggatttccat atttgtattc
    35341 ctattaacag gaatcatgat tttctcgcag gtacttttta tcatctatgc tatgtttctg
    35401 cctgtgagct ttattttaag catgattcca tcatttgatg gtatgtcaaa acgagccata
    35461 acaaagctct ttaataccat tttgacacga gctggaatca cattgattat tacgacagca
    35521 tttagtattt caaccatgct ctatacctta tcggctggtt atccgttctt tttgattgct
    35581 tttctacaga ttgtgacctt tgcaggaatc tactttaagc tgggcgattt aatgagtatg
    35641 ttttctctac agagtaacga ttctcaaagt gtgggaagtc gtatgatgag aaaacctcgt
    35701 atgcttatgc acgctcacat gcaccgtcta cagcggaaac ttggacgctc cgtgactgct
    35761 ttaggggctg ggtctgccat tgccagtgct acgggtaaaa aaggacagtc gggttcggga
    35821 agttctgcga gtacacaagc agatcactct cgaccaaacg ggcaggaaaa atcaacacta
    35881 ggaaagcgta tcggtcaaac catcggtaca gtagctgata ccaaagacag aatggtagat
    35941 actgctggta atttgaaaga acaggttaag gatttgccga ccaatgcaag atatgcagta
    36001 tatcaaggaa aatctaaagt gacggataat gtccatgatt taaccagaag tatttctcaa
    36061 accaaagcgg ataaagccag tggacgcaag aaacagcagg aacaaagacg gaaaaccatt
    36121 gcggagcgtc gctctgaaat ggaacaggtc aaacagaaaa aacagccagc ttcttccgtt
    36181 catgaaagac cagccactaa gcaagaacaa tctcatgatg gacagacttc aaggcaaaca
    36241 actgtacagg cttcctatag ggaatctcaa caagccaaac aagagcgtcc aacagttaag
    36301 tccgattctt caagtttaaa aacggaacgt caaagtaata cagttcaaga aagaacggtt
    36361 caaaagccag taacttcaac tgcaccaaca gatagagctt cacaacgttc aatcacaaaa
    36421 gaacgtcagt ctactgttca aagagtacca ctacaaaata caaaaagtag accaccaatc
    36481 aaaaccgcca ccattaagaa aggcagtaag aaaccatgaa gctgaaaact ttagtgattg
    36541 gtggttcggg attattcttg atggtcttct cactgcttct gtttgtcgcc attttatttt
    36601 cagatgaaca agatggcggt ttttccaaca ttcactatgg cggtgtggat gtttccgcag
    36661 aagtgctggc tcataagcct atggtagaaa aagttgccaa agaatatgga attgaagaat
    36721 acgtcaatat acttcttgcg attatacagg tggaatcggg tggtacagca gaagatgtta
    36781 tgcagtcctc ggaatccctc ggtcttccac ctaattcatt gagtacagaa gaatccatta
    36841 agcaaggtgt gaagtatttc agtgagttat tagccagtag cgaaaggctt ggtgtagatt
    36901 tagaatcggt tatccagtcc tacaattatg gtggtggttt cttaggatat gtggctaatc
    36961 gtgggaataa atataccttt gaactggctc aaagtttctc aaaagagtat tcgggtgacg
    37021 aaaaagtgtc ttaccccaat cccatagcca tacctatcaa tgggggctgg cgatacaatt
    37081 acgggaatat gttttatgtg caactggtaa cgcagtatct tgtcacaaca gagtttgatg
    37141 atgatacagt acaagccatc atggacgaag ctctgaaatt tgaaggctgg aagtatgtct
    37201 atggcggagc ttccccgact acctcttttg attgtagcgg actgacacaa tggacgtatg
    37261 gaaaagctgg aatcaactta ccacgaacag cacaacagca atatgatgta acccggcata
    37321 tcccattatt ggaagcaaaa gccggcgatt tggttttctt tcattctacc tataacgctg
    37381 gctcttatat tactcatatc gggatttatt taggcgataa ccgtatgttt catgcaggcg
    37441 accctatcgg ttatgccgac ttaacaagct cttactggca acagcattta gtgggagcag
    37501 gacgaatcaa acaataagaa aggacgattc aatgatgaaa ttcggaaaaa atcagaatac
    37561 agaaaaacag acaccaaagg aaaagaaacc tcgtgtctat aaggtcaatc ctcgtaaaaa
    37621 ggttgtgatt gtcctatggg tacttttagg acttagtttc agctttgcga tattcaagca
    37681 ctttacagcc attgataccc ataccattca tgaaaccacc atcatagaaa aggaatatgt
    37741 cgatactcat catgtggaaa attttgtaga gaactttgcg aaagtctact attcatggga
    37801 actaaacgac aagtccattg ataatcgcat agaaaacctg aaagcatatc tgacagagga
    37861 acttcaagct ctgaatgtcg atacagttcg taaagatatt cctgtatcgt cttctgtaaa
    37921 aggatttcag atatggacgg tagaagcaga tggcgacaat caatttgatg taacctacag
    37981 tgtagaccaa ctcattacag agggggaaaa tacaaagacc gtccactctg cttatatagt
    38041 gagtgtctat gtagacagta ctggaaatat ggtcctcatt aagaatccga ccattactag
    38101 cataccaaag aaatcagact ataaaccaaa agctattgaa agtgagggta cggttgattc
    38161 cattacaacc aatgaaatca atgagttttt aacgacgttc ttcaagctct atcctacagc
    38221 gacagccagt gaactttcct actatgtgaa tgacgggata ttaaaaccaa tcggaaaaga
    38281 gtacatcttt caagaactgg taaatcctat ttacaatcgt aaggataatc aagtcacggt
    38341 atcgctgaca gtggagtata tcgaccagca gaccaaagca acgcaggtat ctcaatttga
    38401 tttggtactt gaaaagaacg ggagtaattg gaagattata gaataacaaa tattggtaca
    38461 ttattacagc tattttgtaa tcacgtactc tctttgataa aaaattggag attcctttac
    38521 aaatatgctc ttacgtgcta ttatttaagt atctatttaa aaggagttaa taaatatgcg
    38581 gcaaggtatt cttaaataaa ctgtcaattt gatagtggga acaaataatt ggatgtcctt
    38641 ttttaggagg gcttagtttt ttgcacccag tttaagaata cctttatcat gtgattctaa
    38701 agtatccgga gaatatctgt atgctttgta tgcctatggt tatgcataaa aatcccagtg
    38761 ataagagtat ttatcactgg gatttttatg cccttttggg cttttgaatg gaggaaaatc
    38821 acatgaaaat tattaatatt ggagttttag ctcatgttga tgcgggaaaa actaccttaa
    38881 cagaaagctt attatataac agtggagcga ttacagaatt aggaagcgtg gacaaaggta
    38941 caacgaggac ggataatacg cttttagaac gtcagagagg aattacaatt cagacaggaa
    39001 taacctcttt tcagtgggaa aatacgaagg tgaacatcat agacacgcca ggacatatgg
    39061 atttcttagc agaagtatat cgttcattat cagttttaga tggggcaatt ctactgattt
    39121 ctgcaaaaga tggcgtacaa gcacaaactc gtatattatt tcatgcactt aggaaaatgg
    39181 ggattcccac aatctttttt atcaataaga ttgaccaaaa tggaattgat ttatcaacgg
    39241 tttatcagga tattaaagag aaactttctg ccgaaattgt aatcaaacag aaggtagaac
    39301 tgtatcctaa tatgtgtgtg acgaacttta ccgaatctga acaatgggat acggtaatag
    39361 agggaaacga tgacctttta gagaaatata tgtccggtaa atcattagaa gcattggaac
    39421 tcgaacaaga ggaaagcata agatttcaga attgctcctt gtaccctgtt tatcatggaa
    39481 gcgcaaaaag caacataggg attgagcagc ttatagaagt gataacgaat aaattttatt
    39541 catcaacata cagaaagaag tctgaacttt gcggaaatgt cttcaaaatt gaatattcgg
    39601 aagaaagaca acgtcttgca tatgtacgcc tttatggcgg aatcctgcat ttgcgggatt
    39661 cggttagaat atcggaaaag gaaaaaataa aaattacaga aatgtatact tcaataaatg
    39721 gtgaattatg taaaattgat aaggcttatt ccggggaaat tgttattttg caaaatgagt
    39781 ttttgaagct aaatagtgtt cttggagata caaagctatt gccacagaga gagagaattg
    39841 aaaatccgct ccctctgctg caaacaactg ttgaaccgag caaacctcaa caaagggaaa
    39901 tgttacttga tgcactttta gaaatctccg acagtgaccc gcttctacaa tattatgtgg
    39961 attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta
    40021 gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct acagtcattt
    40081 atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg ccgccaaatc
    40141 ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt ggaatgcagt
    40201 atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca gttatggaag
    40261 ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat tgtaaaatct
    40321 gttttaagta tggcttatac tatagccctg ttagtacccc agcagatttt cggatgcttg
    40381 ctcctattgt attggaacaa gtcttaaaaa aagctggaac agaattgtta gagccatatc
    40441 ttagttttaa aatttatgcg ccacaggaat atctttcacg agcatacaac gatgctccta
    40501 aatattgtgc gaacatcgta gacactcaat tgaaaaataa tgaggtcatt cttagtggag
    40561 aaatccctgc tcggtgtatt caagaatatc gtagtgattt aactttcttt acaaatggac
    40621 gtagtgtttg tttaacagag ttaaaagggt accatgttac taccggtgaa cctgtttgcc
    40681 agccccgtcg tccaaatagt cggatagata aagtacgata tatgttcaat aaaataactt
    40741 agtgtatttt atgttgttat ataaatatgg tttcttgtta aataagatga aatatttttt
    40801 aataaagatt tgaattaaag tgtaaaggag gaattaatta tgaagtgtaa tgaatgtaac
    40861 agggttaaat acaaccaaat tttaatttgg ctttgcgttt tatctttttt tagcgtatta
    40921 aatgaaatgg ttttgaacgt ctcattacct gatattgcaa atgattttaa taaaccacct
    40981 gcgagtacaa actgggtgaa cacagccttt atgttaacct tttccattgg aacagctgta
    41041 tatggaaagc tatctgatca attaggcatc aaaaggttac tcctatttgg aattataata
    41101 aattgtttcg ggtcggtaat tgggtttgtt ggccattctt tcttttcctt acttattatg
    41161 gctcgtttta ttcaaggggc tggtgcagct gcatttccag cactcgtaat ggttgtagtt
    41221 gcgcgctata ttccaaagga aaataggggt aaagcatttg gtcttattgg atcgatagta
    41281 gccatgggag aaggagtcgg tccagcgatt ggtggaatga tagcccatta tattcattgg
    41341 tcctatcttc tactcattcc tatgataaca attatcactg ttccgtttct tatgaaatta
    41401 ttaaagaaag aagtaaggat aaaaggtcat tttgatatca aaggaattat actaatgtct
    41461 gtaggcattg tattttttat gttgtttaca acatcatata gcatttcttt tcttatcgtt
    41521 agcgtgctgt cattcctgat atttgtaaaa catatcaaga aagtaacaga tccttttgtt
    41581 gatcccggat tagggaaaaa tatacctttt atgattggag ttctttgtgg gggaattata
    41641 tttggaacag tagcagggtt tgtctctatg gttccttata tgatgaaaga tgttcaccag
    41701 ctaagtactg ccgaaatcgg aagtgtaatt attttccctg gaacaatgag tgtcattatt
    41761 ttcggctaca ttggtgggat acttgttgat agaagaggtc ctttatacgt gttaaacatc
    41821 ggagttacat ttctttctgt tagcttttta acagaattag gcagaagtcg cccatcctct
    41881 ataggtgggt gatgaatgcc gttcggtatg agggttaccg ttggtaactc gaagaccgac
    41941 aactaaggcg tat
//
LOCUS       DAXWLF010000003        41783 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXWLF010000003 DAXWLF010000000
VERSION     DAXWLF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.003"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            173..2338
                     /gene="acm"
                     /locus_tag="ACGE4U_000099"
     CDS             173..2338
                     /gene="acm"
                     /locus_tag="ACGE4U_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFF8392297.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2610..3095)
                     /locus_tag="ACGE4U_000100"
     CDS             complement(2610..3095)
                     /locus_tag="ACGE4U_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFF8392298.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3185..3530
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3575..4216
                     /locus_tag="ACGE4U_000101"
     CDS             3575..4216
                     /locus_tag="ACGE4U_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8392299.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4235..4870
                     /locus_tag="ACGE4U_000102"
     CDS             4235..4870
                     /locus_tag="ACGE4U_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8392300.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4888..5703
                     /locus_tag="ACGE4U_000103"
     CDS             4888..5703
                     /locus_tag="ACGE4U_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8392301.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6022..7458
                     /locus_tag="ACGE4U_000104"
     CDS             6022..7458
                     /locus_tag="ACGE4U_000104"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8392302.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7647..10094
                     /locus_tag="ACGE4U_000105"
     CDS             7647..10094
                     /locus_tag="ACGE4U_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFF8392303.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10324..11391
                     /gene="rlmN"
                     /locus_tag="ACGE4U_000106"
     CDS             10324..11391
                     /gene="rlmN"
                     /locus_tag="ACGE4U_000106"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFF8392304.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11381..11587
                     /locus_tag="ACGE4U_000107"
     CDS             11381..11587
                     /locus_tag="ACGE4U_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392305.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11584..12687)
                     /locus_tag="ACGE4U_000108"
     CDS             complement(11584..12687)
                     /locus_tag="ACGE4U_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8392306.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13240..13788
                     /gene="aac(6')"
                     /locus_tag="ACGE4U_000109"
     CDS             13240..13788
                     /gene="aac(6')"
                     /locus_tag="ACGE4U_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFF8392307.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(14348..16240)
                     /locus_tag="ACGE4U_000110"
     CDS             complement(14348..16240)
                     /locus_tag="ACGE4U_000110"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8392308.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(16237..17964)
                     /locus_tag="ACGE4U_000111"
     CDS             complement(16237..17964)
                     /locus_tag="ACGE4U_000111"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8392309.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17980..18630)
                     /locus_tag="ACGE4U_000112"
     CDS             complement(17980..18630)
                     /locus_tag="ACGE4U_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFF8392310.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18834..19130
                     /locus_tag="ACGE4U_000113"
     CDS             18834..19130
                     /locus_tag="ACGE4U_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8392311.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            19322..19942
                     /locus_tag="ACGE4U_000114"
     CDS             19322..19942
                     /locus_tag="ACGE4U_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8392312.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(20034..21200)
                     /locus_tag="ACGE4U_000115"
     CDS             complement(20034..21200)
                     /locus_tag="ACGE4U_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8392313.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(21212..21565)
                     /locus_tag="ACGE4U_000116"
     CDS             complement(21212..21565)
                     /locus_tag="ACGE4U_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFF8392314.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            21693..22715
                     /locus_tag="ACGE4U_000117"
     CDS             21693..22715
                     /locus_tag="ACGE4U_000117"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFF8392315.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            22734..22895
                     /locus_tag="ACGE4U_000118"
     CDS             22734..22895
                     /locus_tag="ACGE4U_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392316.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(23154..23588)
                     /locus_tag="ACGE4U_000119"
     CDS             complement(23154..23588)
                     /locus_tag="ACGE4U_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392317.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(23698..25557)
                     /locus_tag="ACGE4U_000120"
     CDS             complement(23698..25557)
                     /locus_tag="ACGE4U_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8392318.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMVLFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            25558..25698
                     /locus_tag="ACGE4U_000121"
     CDS             25558..25698
                     /locus_tag="ACGE4U_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFF8392319.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(25675..26834)
                     /locus_tag="ACGE4U_000122"
                     /pseudo
     CDS             complement(25675..26834)
                     /locus_tag="ACGE4U_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(27141..28565)
                     /gene="araA"
                     /locus_tag="ACGE4U_000123"
     CDS             complement(27141..28565)
                     /gene="araA"
                     /locus_tag="ACGE4U_000123"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFF8392320.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(28581..29279)
                     /locus_tag="ACGE4U_000124"
     CDS             complement(28581..29279)
                     /locus_tag="ACGE4U_000124"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFF8392321.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(29236..30897)
                     /locus_tag="ACGE4U_000125"
     CDS             complement(29236..30897)
                     /locus_tag="ACGE4U_000125"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFF8392322.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            31115..32515
                     /locus_tag="ACGE4U_000126"
     CDS             31115..32515
                     /locus_tag="ACGE4U_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFF8392323.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(32767..33846)
                     /locus_tag="ACGE4U_000127"
     CDS             complement(32767..33846)
                     /locus_tag="ACGE4U_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8392324.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33932..34891)
                     /locus_tag="ACGE4U_000128"
     CDS             complement(33932..34891)
                     /locus_tag="ACGE4U_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFF8392325.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34903..36414)
                     /locus_tag="ACGE4U_000129"
     CDS             complement(34903..36414)
                     /locus_tag="ACGE4U_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFF8392326.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(36436..36999)
                     /locus_tag="ACGE4U_000130"
     CDS             complement(36436..36999)
                     /locus_tag="ACGE4U_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFF8392327.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(37005..37868)
                     /locus_tag="ACGE4U_000131"
     CDS             complement(37005..37868)
                     /locus_tag="ACGE4U_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8392328.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37882..38778)
                     /locus_tag="ACGE4U_000132"
     CDS             complement(37882..38778)
                     /locus_tag="ACGE4U_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8392329.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38953..40242)
                     /locus_tag="ACGE4U_000133"
     CDS             complement(38953..40242)
                     /locus_tag="ACGE4U_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8392330.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(40214..41731)
                     /locus_tag="ACGE4U_000134"
     CDS             complement(40214..41731)
                     /locus_tag="ACGE4U_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFF8392331.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        12901 a         8172 c         7784 g        12926 t
ORIGIN      
        1 aaccttgtaa aaacagtttt ttggcatatt tattttttat aaatatttgt aaaataacta
       61 tcaatttttt agttaaaaga ttggaaatga gtgggggagg aattgaagaa aggatttttg
      121 agagatgata tagtaggttt tatttttagt taaaataggg gagatagaaa aaatgaaaaa
      181 gtgtagaaat attattttat caatgttatt tattattact aatataacat cactgattcc
      241 tgttcatgtc tatgcggatg caggcagaga tatcagcagt aatgtcactt cgttgaccgt
      301 ggacccaacg aatattacag atggaggaaa cataaaagta aaattctcgt ttgacgaaaa
      361 aaaacaaaat atccaaccag gtgattatct ttggatcaat tggccaagtg aaggaaatat
      421 tcgcggtgaa ggattccaaa aagaaatccc attaatgatt gaaaataaaa atgttggaac
      481 tttaacggtc agaaaagatt ctgcgcaggt tgtgtttaat gaaaatatta aaaacctaga
      541 ctcagtagaa ggttgggggc aatttgagat acaggccaga aacgtaaccg ataccggtga
      601 agaaaatacg ggaccattca ccgtgacgag cggtgataaa acagctactg taaatgtgac
      661 aaagccagct tctggttctt cttctagcgt tttttattat aaaacaggag acatgctacc
      721 tgaagatact aagcacattc gatggttttt gaatattaac aataacggca cttatgttga
      781 acaaccagtt aaaatcagtg atgaaatcca aagtggacaa agattagatc caagtacgtt
      841 tgaaattaat caaatacact taggtgaaca gaaggtttat agaggagaag agggaatcca
      901 acaattttta caagatttcc ccagcgctac atttaatttt tcagtaactg ataattacat
      961 tgaaataact ataccaaaaa actttgtcaa tcttaggaaa ataatggtga gttataagac
     1021 gatcatagaa aacccagaac aaataaattt tgagaatcat tcggaagctt ggtttaaaga
     1081 atttaataaa cctgctgttg atggagaaag tttcaatcat acagttaaaa atatttcagc
     1141 ttctggtgga gtcaacggta cagttagagg cgaattgaag attttcaaat atatcaatga
     1201 cacagagatt ggtatcccaa atgtgacttt cgaattaaga cgagcagatg aacagcctat
     1261 tcaaggacag tctagtatcc tgttgacttc gaatgaacag ggagaagcta gtatcaaagg
     1321 cctgcaagta ggagactatg ttgtaaaaga aaaagaagca cccaattgga ttgattttga
     1381 tccactaagt tcaaatgaac tcaaattctc cataaacgaa aatgatacag agggagtcag
     1441 tttacctatt tataacaaga aaaaggttac gaacatcaca gcaactaaaa tttggaacgg
     1501 tggaaccact ccaagaccga gtatttattt taaattattc agagcaagta caaacaattg
     1561 ggaaccagtt cctgatgcag aaacaaaacg attagacaat ggaataactt cagtgacttg
     1621 ggaagatatc caacagtatg atgatagtgg aaacgaatat acgtttaaag tgcaagaagt
     1681 agatcaaaac ggaaatgatt atgtcccttc aggttatcaa aaaattgaaa acgggctagt
     1741 cgttacaaat gagaataaag aagtcatttc agtctcagga caaaaaacat gggaagacaa
     1801 tgagaaccaa gacggcaaac gaccgacgac gatcacagta aatctattag ccgacggtca
     1861 accgatccaa cacaaagaag taagtgaaaa agatgattgg cgctatcgtt tcacaaatct
     1921 gccaaaatac agagacgggc aagaaatcat ctatactgta acggaagaca acgtgcccga
     1981 atacagtacg acgattgacg ggtataacat caaaaacagt tatacaccgg aaaaaacaaa
     2041 tatatctata ccaggtagcc atataacacc gtggaaaaat tttccagaga agacagagag
     2101 agagaaaatt agaaatctat tctctcaaaa attgcaaata tttgctcata caggagaatc
     2161 cagcgaacaa agaagcaatt atattagtaa gagagctgta cctaaacaga taacaaataa
     2221 atcaaaaagc attcttccta aaacaagtag taaaaaatct agttttgctg taatcattgg
     2281 tttattgata gtaactatct gtgctgggat attcttacaa aaatataaaa aagtttagtt
     2341 agaatataat attttttgtt aaatatgact gaaatcaaaa gaataaaatt ccgatttata
     2401 atcgaaaaaa gaagcatttt tatatatgct tcttttttcg tagaatcaat aaatatggat
     2461 ttggctaact atggaagaat actcgtatta gttttcagcg ttcatactag ttgttacatt
     2521 gtttgattgg ccaaaagaat ttctgatata ttttgtgcta aataaagaaa aagacgcttg
     2581 aattgggaaa taagaaaact actctatcat tacaaatttt cataaactgg cagatgttct
     2641 aagtaaacaa gtatcttttc aaagtcggaa aaatccataa tcaacgtagt ggtattgata
     2701 tttggatgaa agcctacggt atcttgacga tcaatagcag catcgatcac gacttggatt
     2761 tttttctctt tgtcgtgcat caatgctaat ggagtcactg tacctgctgg aacgcctagg
     2821 aggtcaggaa gctgttcttc agaaacaaaa gataagcgct tttcttttaa ttgttcagct
     2881 agttgtttta aatcggcgtg cttttcatca ggcaagatga caagatagat ttgttttcct
     2941 tttcttgatt tcaataatag attttttact tgcggaccag gtagggaaaa agggacattt
     3001 ttgactgacg taatcgcagg atgatcgact ttttgaaaag agatacccag tttttttagt
     3061 agttcataag cttcttgttc ggttgcacga ctcatcgcgc atcaccctcc tttttttatt
     3121 agtgtatcat aaaaaagagt tgacaaatga aaaataatca tgtaaattac acgtaaagtt
     3181 aaaacaaatg acatcgaaaa gaagagtagc cgttaggtgt ttttagagag tctctggttg
     3241 gtggaaagag gcagcagtta accgtgaaac acatcttgga gtcgattttc tgaagcgtat
     3301 agtaggaaaa tccggggagc ccgttacagc tgcggtcgtt gctagatgac cagataaggc
     3361 tggcttttcc gtgaggagtc ggtaaaaaaa ggtggaacca cgtgattaac gtcctttgat
     3421 cttcggatca aaggactttt tgttttcatc tgaacggcaa cgactgtgtg aggttttttc
     3481 tgcaaggaaa aaataaacag aggtggaacc acgtataata cgtcctcttg gcatttgcca
     3541 agaggatttt tttattttaa aggaggaaaa gaagatggag tatttgattg aagttatgcc
     3601 gcaattatta gtaggagcac tcacaacatt gaaattattt ttatttacct tgattggctc
     3661 attaccatta gggattgttt tagcattagg attgtctttg cattttaagc cgctacgcta
     3721 cttgcttcaa acatatgtat gggttatgcg gggaacaccg ctgttgttgc aactgatttt
     3781 tattttttat gggttgccga cgattgggat cgtttttgat cgtttcgaag cagcactcat
     3841 tgcctttatc ttaaactatg cagcttattt tgcagagatc tttcgtggag ggatacaatc
     3901 gattcctcaa ggtcaatatg aagcagcgca agttttgcaa ttaagtccct ttcaaacggt
     3961 gacgaaaatc gtcatcccac aagtgacaaa gattgtttta ccttctgtag ggaatgaagt
     4021 tatcaacttg gtcaaagatt cttccttgat ctatatatta ggtttaggtg acgtaatgcg
     4081 tgctgggaaa atcgcgatgg aacgcgacgc gactttactg cctttgctag gcgtggcaat
     4141 catctatctt acattgactg gtattctaac aatcgtaatg aaacaagttg aaggaaaatt
     4201 aaattattac cgctaactga taggaggaag aaatatgatt gaattgaaga aagtgaacaa
     4261 agcatttggt gaaaaaaaaa taatcaaaga tttgaatcta atcattccag atgggaaaac
     4321 attagctatc gtaggtcctt ccggtggcgg taaaaccacg cttttacgta tcttggctgg
     4381 cttagaaagt tctgacagcg gagaatttta tttagacggt gtaccgttca acccattcga
     4441 gcttaaaatg aaagaacaag ttgtaggagt cgtattccaa gacttccagc tatttccaca
     4501 tctgaatatt tttgaaaaca ttaccttagc accaaaaatg gtattgaaac agacaaaaga
     4561 agaatacacc aaaaaagcag aagaattagc ggcttcatta gggattagtg atttattgaa
     4621 tatctatcct tatcagcttt ctggcggaca aaaacagcgg gtagcgattg ccagagcatt
     4681 ggctatgaat ccacgggtat tggcttatga tgaaccaaca agtgctttag atcctgaatt
     4741 acgccagcaa gtcgagcgtt tgatcctatc attgaaagaa gatggggtaa cccaaattat
     4801 tgtcacccat gatttagtat ttgcagaaaa tgtcggtgat caattactaa aagtttcccc
     4861 aattcattaa gaaaggagaa gatagagatg aaaaaaacca gttttctatt cgctttgatc
     4921 gcaggtcttc ttctttttgc aggctgttcg aacaaaaaaa cttcagcaga taattgggcg
     4981 actatcaacg aagagaaaaa aatcgttata ggtttagacg atacatttgt tcctatggga
     5041 tttagagata aagacgggaa gctcacagga tttgatattg atttagccaa agcagtcttt
     5101 gaacaatatg ggattacggt agactttcag cccattgact ggtcaatgaa ggaatttgaa
     5161 ctgaacaatg gaacgatcga tttgatatgg aacggctatt ccaaaacgcc ggcaagagaa
     5221 aagaaagtgc aatttacaaa accttatatg gaaaacgatc aagtgctgat cacacctaaa
     5281 gaatcaggaa tcacaaactt tggacaaatg aataataaga gattaggtgc gcaaaatggg
     5341 tcatctggtt atgatgtatt tacgaaacaa ccagaagtat taaaagagat agtcaaaaac
     5401 caagatgctg ttttgtatga tagttttaat gaagcattga tcgatctgaa aagcagacgg
     5461 gtcgatggtc tgctgatgga taaagtatac gcagactatt atttgaaaca aagaaacgaa
     5521 ctagaagact tcaacataac aaaaggttct tatgagagtg aggattttgc agttggtgca
     5581 cgcaagtcag atagtacatt agtgaaaaaa atcaatgaag ccctagatca actagagaaa
     5641 acaggtgaat tccaaaaaat atccgataaa tggtttggcg aagatgttac gccgagaaat
     5701 taacaaatag gggactgtga caaaagacgt gtcacagtcc ctttttccga ataaacagtg
     5761 ttctcctgcc aactccttgt gactcggagc tgaatggcag gctcacaacc tcttggtaaa
     5821 cggtgttcaa aagccagttt ttcggtatta agccaaattt atgcaaaaat gtagaaagcc
     5881 attttcacaa aatttttctt actactcgaa actaagcgac tttttcacaa cctcctttac
     5941 atatttttat tttgttgtgg aaggggtttc gtgttattct aaaagaggaa cgattgaagg
     6001 ttataggaaa ggaagagatg aatggaacac aaagaactta cctcatttcc aaaagatttt
     6061 ctgtggggct ctgcttctgc agcgtatcaa gtagaaggag cttggcaaga agatgggaaa
     6121 ggagaatcag tctgggatcg atttgtacgg atacctggaa agacatttaa aggaacgaac
     6181 ggtgatttgg cagtagacca ctatcatcgt tataaagaag atattgcttt aatgaaagaa
     6241 caaggtctga aagcttaccg cttctctgta gcttggacac ggatttttcc taatggacga
     6301 ggagaagtca atcaagctgg gctcgctttt tatgagagat tgatcgacga attgattgaa
     6361 aacgatattg aacctatgtt gactttatat cattgggatc ttccacaagc attgcaagat
     6421 gaatataatg gttgggaatc acgtcagatc attgaagatt tcactcatta tgcagaaaca
     6481 ttatttgagg cattccgcgg gaaagtccat tactggatta gtttgaatga acaaaatatc
     6541 ttcacttcat taggttattt gttagcagca catccgccag gtgtgacaga cccgaaacga
     6601 atgtatgaag ttaaccatat tgctaattta gcaaatgcat ctgtcatcaa taaatttcac
     6661 gagttagaaa tgcctggaaa aatcggacca agctttgcgt atacgccaaa ttatccaatt
     6721 gacagtaatc ctgaaaatgt tctggctgct gaaaatgcag aagacctgat ggccaattat
     6781 tggatggatg tctatatgtg gggaaaatat ccgattgctg ccatgaggtt cttagaagaa
     6841 aaaggatggg caccaacgat tgaagctggc gatgcagaat tgctagaatc tgctaaacca
     6901 gattttcttg ggatcaatta ctatcaaacg gcgacgaatg cattcaatcc attaaacgga
     6961 gttggtgctg gaaaaatgaa tacgacagga aaaaaaggtt catcagaaga aactggagtt
     7021 cctggaatgt ataaaaaagt tgagaatcca tttgtcgaac gaacaaattg ggactgggag
     7081 attgacccag aaggattaag gatcggactg cgtcgtatca caagccgtta ccgcatgccg
     7141 gtactgatta cggaaaatgg cttaggagag tatgacaagt tgacagaaga taaacaaatc
     7201 catgacgact atcgaatcaa ttacttacaa agccacgtca aagccatcaa agaagcaatt
     7261 tcggatggag cagaagtcct aggctattgc acctggtctt atacagattt gcttagctgg
     7321 ctgaacggtt atcaaaagcg atatggattt gtatatgttg atcaagacga aacacaagaa
     7381 ggatctttgg aacgatataa gaaagacagt tattactggt accaaaaact gattgaagaa
     7441 aatggagaga acctttagtt cacatactta aaacaagaag gtacggctgg atatggccgt
     7501 gccttttctt gataaaatca tttagaaatt aagaaaaact gtaaaataag ctgaaaaatg
     7561 aaagaaagcg tcagttcttt caaacaaaac tttagttccc ttactttttt ttgtataatg
     7621 aaacttgatg aaatgaggga taataaatgg cacaaaagaa acggcctact aaaaaaagga
     7681 aaaagacgaa aaaaacgaaa aagcaaaaac agcagcagga acaaatgctg acgattggtg
     7741 ttgggtttgc cttgatttta tttgctgtct tcggcttttt aaaattaggt tttttaggaa
     7801 tattgattgc aaacggcttt cggatcattg caggaaatac ttaccaaatc ctctgtcttt
     7861 tattagccgt attaggattt tggattgtca ttaagaatac agagttttca attggtaaaa
     7921 accgacgctg gtttggcggc atactctttt atttcggtat cttattattg ttacatgccc
     7981 atttatttgg caaactccat actggggaac caaatatcat ggggacgaca tgggatttgt
     8041 tagccagtga tataaaacaa agtcaagtag acaataacgt tggtggtggg atgattggag
     8101 caatccttta tcattttact tacttcttga ttgcacagcc tggaagttat ttagcagcag
     8161 tgctattact ggcaggagga gcattcctgt tcagtaatct ggaaggctat cagctgttaa
     8221 atggtatcca atcaattgga gaacgagtac aggaattatt ggaaggtgat cctgccaagc
     8281 aagctcgaaa gcaagcggca aaggaagaga gaatgaaaca gcgcgcagaa gcgaaagaag
     8341 caaggcgctt agctgcaaaa gaagctgcag aaaaagaagc tgcagaatat gagaagaaaa
     8401 aagcggtgag ccagcaaagg gaaaatactg ctgatgaatg gcaggaacca gaaaatacag
     8461 agccggagca gctaagtttt gttccaatcg acagttttca agaaaatatc catcctgcga
     8521 atctagagaa accagtacca gatacaccaa aacagaccaa tacagcagaa ggtttttcag
     8581 atgagttgcc agaggatgac ggtacctcat tagaatttga gattgaagca gaacaggaaa
     8641 atcaagacta tgaattaccg acagttgatt tgcttgattc tataccaact gtggatcaaa
     8701 gtgatgagta taaaaaaatt gaaaaaaata tcggcgtatt agaacaaact ttccaaagtt
     8761 tcggtgtcga tgccaaggtc gtcaaagcca gcttaggacc atccgtcaca aaattcgaag
     8821 tccagccggc tgtaggggta aaagtaagta aaattgtgaa cttaacagat gatatcgcgc
     8881 ttgctttagc ggcgaaagat gtccggatgg aagcaccgat tccaggtaaa tcactgattg
     8941 gtattgaagt accaaatgga aagatcagta tggtttcctt tagagaaatc atcgaagcac
     9001 aaccaaacca cccagataaa ttactagaag tacctttagg acgtgatgtt tctggacggg
     9061 tacaaacggc tgatctttcg aagatgcctc acttactcgt ggctggttcg actggtagtg
     9121 ggaagtcagt agcaattaat gggattatca caagtatatt aatgcgtgct aagccccatg
     9181 aagtaaaact gatgatgatc gatccgaaaa tggttgaatt gaatatgtac aacggcatac
     9241 ctcatctgtt gacgccggtc gtgacgaacc ctagaaaagc agcgcaagcc ttacaaaaag
     9301 tagtacaaga aatggaagaa cgatatgaaa aattcgcggc aaccggagta agaaatattt
     9361 ctggttacaa tgaatttgtt caacagaaaa acttggaaaa cggaacgaaa catccaacct
     9421 taccattcat cgtagtgatc gtagatgaat tagctgattt gatgatggta gccagcaatg
     9481 aagttgaaga tgcgattatt cgtttagcgc aaatggctcg ggcagcgggg attcatatga
     9541 tcctagctac tcaacgtcca agtgtggatg ttatcacagg aatcatcaaa gcaaatgtac
     9601 cttccagaat ggcatttgcg gtatcaagcg gtacagattc tcggacgatc attgattcta
     9661 atggggcaga aaagctctta gggcgaggag atatgctatt cttgccaatg ggtgagaata
     9721 aaccgatccg tgtgcaagga gcattcattt ctgaccacga agtagaacgg gtcgttcaat
     9781 ttgttacaga tcagcaagaa gctcattatg aagagaaaat gatgccgaca gatgaagttg
     9841 aaacgacagg tgctccagaa cagccacaag atgagctgtt tgaagaagca aaagcacttg
     9901 tcgttgaaat gcaaacggca agtatttccc ttctccaaag aagatttcgg atcggttaca
     9961 accgagcagc acgtttagta gatgaactgg aagctcatgg agttgttggc ccgtcagaag
    10021 gaagcaagcc tcggaaagta ttcattgagc aggaagaaac atcagaaacg ttgccggacg
    10081 ataatgacat gtaaaacttg tgtgatgaag ggttgaaaaa cgaataacca ataaaaagac
    10141 aaggcatgat gataccaaaa gaaaaaatgg tttttttcca tcttttctaa tacggtatga
    10201 tgtcttgtct ttttagtttt tgtgagatta aagttctatt cgatttattt gtgaagtatt
    10261 gcattaagga aatgaatcat gtatagtatg tagcgaaact ttgagaaaaa gagggaaaat
    10321 actttggaaa aacagtccat ttatggcttg acgaatgaag aattaatcaa ttggtttata
    10381 gaaaacggcg agaaaaaatt ccgagctgcc caagtttggg aatggttgta tcaaaaacgt
    10441 gtaagtaatt tcacagaaat gacgaatcta tcgaaacaat tgatcgaaaa attatcggag
    10501 cattttatca tcaatcctct aaaacagatg gtcgtccaag aagcttcgga tggtacagta
    10561 aaatatttgt ttgaactccc tgataaaaac atgattgaga ctgtactgat gagacaagaa
    10621 tatggcttgt cagtttgtgt gacgacacaa gtaggatgta atatcggctg tacgttttgt
    10681 gcaagcgggc tgctcaaaaa gaaccgtgat ctgactgctg gagaaattgt tgcacagatc
    10741 atgatggtcc aacattattt tgatgagcgc aatcttggcg aacgcgtttc acatgtcgtt
    10801 gtaatgggga tcggtgagcc atttgataat tacgacaatg taatggactt tttgcatatc
    10861 atcaatgatg ccaaaggatt ggcaattggt gctcggcaca ttacagtttc cactagtgga
    10921 ttagcacaca aaatcaagga attcgctaat aacggcttgc aagtcaatct ggctatttcc
    10981 ttgcacgcgc cgaataatga ggtgcgaaca tcgatcatgc ggatcaaccg aagtttccca
    11041 attgagaaat tgatggaagc tgtggatgag tatttagaga aaacaaaccg tcgaatcacg
    11101 tttgaatata tcatgttgaa tcaagtaaat gatagaccag agcatgcgca acaattagca
    11161 gatctattaa aagataagaa aaaattagca tatgtcaact tgattccata taatccagtc
    11221 agtgaacatg atcaatattc tcgcagtcca aaagctgatg tattgagatt ttatgatgta
    11281 ttgaagaaaa acggcgtaaa ctgtgtgata cgtaaagaac atggtacaga tattgatgca
    11341 gcttgcggac aattgcgaag caagcaaatg aaaaaaacag ttgccaaata atcgtatcct
    11401 actcgttttt tcaattacca taaaaggagc agttttctta attaaggaac tgttcaactt
    11461 aaataagaga aaagattcta tagcgaaagc tataaagctt tttttgacaa gaaaaaccct
    11521 catcaagcaa agaaatgtcc tcaatttgat gacagcctgc ttgatggggg ttttagcaat
    11581 tatttaactg attttcttat atccttgctg gctttcttgt gtagcatgat aagtttctac
    11641 gtcaagaatg gcctgtttaa atttttctcg ggtgatttca taaggcacat gtttaatatc
    11701 atttgttgcc atggctttat ctaagattgc ttctaaatcg gtcgtgacat tgagattcat
    11761 gtcggttaac gatactggaa gttggttctt tttcataaac acatagattt tatcaaacaa
    11821 atcgaactgc tgatccatca tcaataaggt cagtacacca taactgacag atgctccgtg
    11881 aaggtatccc tccatttttt ctagaacagt cgtaccgtaa tagaatgagt gtgccatagc
    11941 agaattatag ttattgttaa ctaaaacaga aaccaatccg gtagtaacaa tgatatccga
    12001 aacgacttgt tcaattgcat atgagggacg atcgtcggca ctatctttca atgcttgcgt
    12061 accataagtc aagagacgat caatgcacat ttttccgatt tggattccta atctatccgt
    12121 gtagcctaga tcatcgccac gagcagaaaa agtagattcg acttctttgg ataaagcatc
    12181 gccaatacct gcccataagt atttatcagg tgcttcggca ataattgctg tatcaatgaa
    12241 ggtgtgtaca ggtggttttg aaggaaagaa gagtccgtcc attacgcctt cttcgttata
    12301 gatgacacat accgcagtga cgggcgaaca gttcgaacca atagtgggaa aggtgaagac
    12361 tggtttagct agtttttcac caatgacttt tacagtatcg caagcacggc caccaccgac
    12421 tgcaaacagg acgtctgctt tttggacact ttcatgctgg atcaactgtt caatatttcc
    12481 ataagttgaa ttacccccgt accagagact ggcgacaacc tctatttcct gtgtcaaagc
    12541 tacttttatt ttgttttctg cctttgaaag tgcagtcttc cctccaatca gcactacttt
    12601 tgtcccatat tctttgacga ttccatttgc tttttcatac gcatctgttc caatggtata
    12661 atttggtaag aaaacacttt gactcatatt catttccttc tttcttcgtt gaaacgggtt
    12721 aaaattttaa agagtgatcg atctgactgc ggctgtagat ggagtagagg tgaacctaag
    12781 acaggataag tgatgaataa ggtggcatga acaccttgaa atcatcaata agtatcctaa
    12841 aaacgaacaa gtacaaaaac attgaaaacg acaataatga atttttgttc ctcttagtat
    12901 ttctaccgat tccctttgct tttttgtaat tatctaaaaa ttctaaaaat taagattatt
    12961 atatgaaaac aggttttatt tgtcaattca taattggttt tttttaaagc aaaagataat
    13021 ctatatcatg atgaacaaac agagtttttt cctccaattg atggctttcc tgatctttcc
    13081 tattttgaat aaagtgtttc tgtataattt ttttatttta taagtaagat aaataatgaa
    13141 agaatagaga tactgattgg tagctaaaga tttttgagcg agaacatttc gttttttcaa
    13201 aaggactgtt agaataagca agaacaaggg ggaagacgta tgataatcag tgaatttgac
    13261 cgtaataatc cagtattgaa agatcagctt tctgatttac tgagactgac ttggccggaa
    13321 gaatatggag acagctcggc agaagaagta gaagaaatga tgaatccaga acgaatcgcg
    13381 gtagcagcgg tagaccaaga tgagttagta ggatttattg gtgcaatccc tcaatacggt
    13441 atcacaggtt gggaattgca tccattagtt gtagaaagct cccgacgaaa gaaccaaata
    13501 ggtactcgat tagtcaatta cttagaaaaa gaagtagctt ccagaggagg aatcacgatt
    13561 tatttaggta cggatgattt agaccatgga acaacgttaa gtcaaacgga cctgtatgag
    13621 catacatttg ataaagtggc ttccatccag aaccttcgtg aacatccgta tgaattctat
    13681 gaaaaattag gttataaaat cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg
    13741 gatatttgga tggcaaaaac gattattcct cgaccaaatt ctcaataata aaaagaggtt
    13801 gtgacagaag taatcagctt caagaaataa gaaggaattt acgaaaatta cttcttgaat
    13861 ttttgtgaaa ttccaactta tttccgaaga agctactttt ggaacaccgt gtaccagagg
    13921 ttgtgaaaaa gtcgcttagt ttcgagtagt aagaaaaatt ttgtgaaaat ggctttctac
    13981 atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg tttaccaaga
    14041 ggttgtgagc ctgccgttta actccgagtc acaaggagca atttcaaaaa acagcttctc
    14101 aggtttgttg actattgtga cttgtgaccg aggagttggc aggagaacac cgtttaagga
    14161 agaggttgta aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct
    14221 tctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat
    14281 tcggaaaaag ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg
    14341 tactattcta tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc
    14401 cgtctttttc catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca
    14461 caataatgtt gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc
    14521 ggttttccaa taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac
    14581 tagatgttgc ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg
    14641 tgattagctg acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat
    14701 atccttctgg caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga
    14761 tttgttcgtc tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa
    14821 acagccaagt atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat
    14881 cacgggtatc tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa
    14941 gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac
    15001 caggtttgac atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt
    15061 gaacattttc aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt
    15121 cattggacat ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg
    15181 attggatagt gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat
    15241 attgcaagaa cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc
    15301 ctagtacagc gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga
    15361 ttgccgaaat aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa
    15421 atacttcctg gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg
    15481 tttcttctac ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt
    15541 gtgatttcgg cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga
    15601 ctaacgtcaa ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg
    15661 cactagttac aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat
    15721 tgattgctcg agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac
    15781 gattcatttt ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg
    15841 tcataataaa ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg
    15901 tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt
    15961 gttgcatctg ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca
    16021 agacctttgg tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga
    16081 tgatcgcaac catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat
    16141 ttttaggctt ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc
    16201 ctgctggtcc atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag
    16261 ttgtgaatga acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc
    16321 tttgccaacc agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact
    16381 tactcgttga gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg
    16441 aagattggca tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc
    16501 tgctgttttg actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt
    16561 tcctcctcct tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt
    16621 agcttgtgcg atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata
    16681 ctgcatattg tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat
    16741 taattctcgc aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga
    16801 accagattcg atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc
    16861 agtcccccct atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa
    16921 tgcaagtttt tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt
    16981 ttttccagca aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc
    17041 caacacttca ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc
    17101 ggaaagcatc ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt
    17161 accgacttcc aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt
    17221 tgtaccgctc atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt
    17281 gattgccgta tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt
    17341 cttatcgaat gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa
    17401 acggtcagtt ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc
    17461 gatcaaaata atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt
    17521 ttgataagca agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa
    17581 aaacatttgt gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga
    17641 ggccgttcca tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc
    17701 cgtacgcaat tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat
    17761 agcagctaag atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata
    17821 atcgacatct ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata
    17881 taaatctgcc agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga
    17941 gatccgtttt acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt
    18001 cagccaagaa agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga
    18061 tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg
    18121 gatcagtaat tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc
    18181 aagcaactct tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg
    18241 actcgtatga ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg
    18301 ataaaaagaa gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc
    18361 gaatcctttg aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct
    18421 gtcacgtctc attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg
    18481 atagaaactg cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt
    18541 taagggtact ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt
    18601 aggcaaatgg aaaaatgtct gtgtcggcat atggattctc ctttctttca agatgacaca
    18661 ttgtcatatg acgctatgtc attataacac ctcctacctg ttttgcaaga catttgcata
    18721 gtttttttca ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa
    18781 ttaatgagag ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc
    18841 aatcaattag tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag
    18901 atacaagaaa taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg
    18961 ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa gaaactttat tatattctaa
    19021 gctatacagg caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg
    19081 ctgtttatat cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg
    19141 ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt
    19201 tttttagtta tttattcttc aaccttatca atatgacttg atagcctttt ctttttcgag
    19261 taaactgatt ttgttagtca tagttataac aatcatagca ataaaaggag aaggtaagca
    19321 tatggcatct gttttagtag tcaaaggtca tcccctaact gcagaagaat cgcgcacagt
    19381 taaggcattg acgtcttttt taacaagtta taaagaaaat catccagatg atgaagtgac
    19441 tgttttggaa ctttatcgcg atgatattcc ggaaatcgat gaagaattat tatcaggttg
    19501 ggaagcatta cgtgctggtg cagaattcac ttccttatca gaaagtcaac aacaaaaaat
    19561 tgctcgtttc aatgaattga ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa
    19621 cgccttatgg aatctaaata tcccaacaaa attaaaagct tggttcgata ccgtcaatgt
    19681 agctggaaaa acgttccgct atacagaaaa cgggccagaa ggattagtga caggtaaaaa
    19741 agcactgcac atccaatcta atggcggtgt atacaacgga caagactttg cctctcaata
    19801 tgtaaaaggt attttaaact ttgtaggcat cgatcaagta gatcagctat tcatcgaagg
    19861 aatcgattat gaccctgacc gtgcagatga gttgatgcaa aatgcattag ataaagctgc
    19921 tgctttaggt aaatcatttt aaaaattgag ataaatgcat acaaaagaga cagaggaaaa
    19981 aatcttcaaa gcagcatttg tgccttggat attttcccat ctgtctcttt ttgttattct
    20041 ttctgctttt tcaacggatt ttcttcataa agagaagaga ttgttgtgta ccattcaaaa
    20101 acatgagtaa tcactacttt agctgctgca taaacaggaa tccccaagat cacaccgatg
    20161 attccgaaca atttcccaga agtcagcaga actaataaaa tcgttactgg atggattgct
    20221 aactgattcc ctagtactaa tggagagatc aagcgtcctt ctattgtctg ctctactgca
    20281 aaaacgatca gtactttgat gatcatgaat ggacctgcta caatggctaa aaagatagcc
    20341 ggaatcatcg ctaaaaatga tcctaagtaa gggatcaggt ttaaaaaacc cgcaataatc
    20401 cccaacgtga cagcatactc taaaccaatg accgcaaaac caatcataaa catgattgcc
    20461 acagcaaaag ccacagttag ttgtcctctt atataagaag aaacttgact attcatttca
    20521 ctcagcacat tcaacgtcgg tttttgcatg cgtgtcggta aaaatttgac aaaataaggt
    20581 gctaattgtt taccgtcttt caatagataa aataagataa acggcatcgt cagcagagcc
    20641 actaaaatag tcgctaccgc gccgacaaaa cttccaagac tctgaactgt actcttagat
    20701 atatcttgga tcatatcgcc tgccgaattc atgaacttat cacctgctgt ttccaactgt
    20761 tcgcgaaatt ggttgaacag cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta
    20821 tctacatatt gagggaagtt actgatgaag ctcactgttt gttcctgaat cttagggatg
    20881 atcactacgc tcccccatgc gattagtgca acaaccaaaa caaacagccc aatgatactg
    20941 taaagccttg gtacttttcg cttttcaaag taatcaacta ccggattcat caagtaatac
    21001 aagattcctg ctaaaatgat aggcagacct acgattgcta aaaattgcca gattggtgca
    21061 aacaaatatg aaacttttgt aaataaaaag acgatcagca atattaacaa tacaacaagt
    21121 aaggctgtga ctacttgatt atttaaaaac cagcgccaaa accacgatac cggtctgttc
    21181 ttcttatttt ttccttccat aattcctcct gttagttata aaaaatttgg cttcccaaac
    21241 caatagcagg cagtctatgc ggatgaagat agattccatt tgcaatttgt ccttcttctg
    21301 gacaagcatc aaaaatcaat gatacgtgag ctactgtctt taaattccca ttggccatcg
    21361 gaccgacata atcgatcgta tatacctgat catcaaaagc aattgttccg cctgctttca
    21421 aagaaaaagg ttcgtctttt gatacttctt gaatcaccga atattttcgt aaggcagctg
    21481 ttgctgtttt atcgaacaaa atcagcatgg gttcttcctt gtttattgct tctgtcccaa
    21541 tttctgtaat ctttgcctga atcattgcac tctctccttt aattgctttc aaaaacagta
    21601 taacacattc tcattcttta gccgtgtgga ataattatgt gatagaataa aaacgaaaac
    21661 tatttcatta caattaggag gaatcagctc atatgatcga aaagataatt ttaggaacct
    21721 atacgcgtcg cgaaagtaaa ggaatctata cgatcgacct tgatacagaa aaagaagaat
    21781 tagccaacct ttcattagct gttgaagaaa atagccctac ctatgtagca aaaagtaaag
    21841 ctggcaatct ttacacagtt actagtgtgg atggattagg tggtgccggt gcgtacgata
    21901 aggattttca ttttttgaat gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg
    21961 ttgatgaagc acgtcaatta ctatacgggg cgaactatca taaaggtgaa gtaaatgtct
    22021 accatatatt aggagatggc gaactgaaag cagcagattc aatattccat caagaagcga
    22081 caggtcctca taaaaaccaa gatcatgcgc atgtccacta tacggatctg actcctgacc
    22141 aacgattagt cgtatgtgat cttggtacag atcgtgtgta tacttatgat gtgtccgaaa
    22201 acggcaaatt gaatcttgta actaccttca ctgcagagcc aggaacaggc cctcgccatc
    22261 ttgtctttca tccaaatgga caattcgctt atttatttgg tgaattagac agcacagtcc
    22321 gcgtgctttc ttataataaa gaaacaggtg cttttgaaga attacagaaa atatcgacct
    22381 tgccagaaga attcgacggt gaaaatggag gggcagctat ccgcatttca aatgatggca
    22441 agttccttta tgcttctaac cgaggacata actcgattgc tgtattcgca gtttttgaag
    22501 atggttccca cattcaaaac aagcaaatca tctcaacaga aggggatttt ccccgcgatt
    22561 tcgcactaga tccaagcaat aattttgtcg tgtgtgccaa tcagaacacc gataatctga
    22621 cattatatcg ccgtcatcct gaaacaggat tactggaatt actgaaaaaa gatgtctatg
    22681 taccagaatg tgtctgtgtt tattttgatc gttagtaaga aaaggagaat gaaatgaatc
    22741 agcaagacgt attagacaga ctacgggaag aactaaatat tcctttcttt gatgggaaaa
    22801 tcgaagataa agagtatagc gaagaggaat atcagaaact caaagccgat ttacagaact
    22861 actttgaaca atatgtacag aatgtagaaa actaatcagc tttccatact atctgaaaag
    22921 aacatcagga caagactttt ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc
    22981 cgagatggtt agactcgacg gaaaaggagt atttactcga ttttgagtaa gtacaaggtt
    23041 gttgttggtt ataggtatgt cattatcata aaggattttg atcactctat cataaaggta
    23101 atattacatt tatattggat aactctagtt tttattgtta tcaaagattt cttttatttt
    23161 ttcaacaaca aattagtcat tttttggatg gcagctgtca attggtctgt cgcttggtct
    23221 tgttttgctt cagatgtatt gaatagatct tcatctaccc ctgattcaag atcgatactt
    23281 tggatcattt cttcacagcc tgctacatat gaaagataag ctttttcaaa atttttatga
    23341 atgcccattg cttttgctgg tggacgcaaa gtactgactt tcataagcat tgcccgatat
    23401 ttttttgttc cttcttcaaa taacaggatg atctcttcaa gagtcgattt gtccagttca
    23461 cttgtttttc cttcatcaat tgcgtttctg atcacttcat atttcggatg cattccttct
    23521 ccgattgctt ctgtttcctt gacgatttga tcgatggact gcacataata gccaatattc
    23581 ggtaacatgt tcgttttcct actttctcca ttgttgttat tatttattct acttgttttt
    23641 atcctcaaat gacaagtact tgaaaaaatt taatggtttc tatacagctc gccattctta
    23701 ttcgtgctca aagaaaccat ctgctaaact atattgtttc cctgcgacaa tgtcatattg
    23761 gatcaattga tattcgtcgt aaatctcttg ttgcgagcga ttgaattgcg cttctttata
    23821 ccactgtccg ttttgatagt acagttctcg ttcaaacgcc ggaagttcct gttctaatga
    23881 taaaagcagc tgataaaagc catttgttgt ctgattcgtc tgttccatta gattcgctgc
    23941 aaaataaaac ggactggtga ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact
    24001 gtcgaacata agaaattgtg tttcatgcaa tgtttgtttg tcattttttg cttggatcgt
    24061 atctgaatag atccctggaa gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag
    24121 attcttcaat tcttccgtga actgcttcag tgccgtgctg ctataggaaa tatcttgcag
    24181 ataattctct aagtcaagtg tcccacttcc atctgaagtt tttgcaggat agtctagatt
    24241 atcatacttt ccatcgtagg gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg
    24301 tgttttttct tccttgagaa gtgtcattac ttctttgtaa gctgactcat cagaaatata
    24361 tggattattc tctatcgtat ccgtataggt catcgtacgc tcgctgatga actggtcaaa
    24421 tcctaatgtc tgataaacat cctctctttt atacatcgaa gtattgtatg gatggatcgc
    24481 tgtcgtctgg tatgattgtg cgtttaacgc tgaaacgata gaaggaagct gatccatttt
    24541 tggtacaagc atcgtgtaag gggtggtgag ttgcgcatta aataacacca tagaaaaacc
    24601 agtcaatgct tcaaattcga tatttgctgt tccaccgcca tagttttgag aaagcatatt
    24661 gccactatat gtttgatcag ctacttcata ataatctgcc aatggctcac cagatacttc
    24721 tacgccgttt aatcgactag gatctgagaa actttcactc atcacgaaca cgatgttcgg
    24781 cgactcttcc tctacagttt tatttttttc atcggctagc ttctggtatt tttcagtgat
    24841 ttctttgatc ttttctttcg aatatccttc aggttcatcc attggttcta ccttcagatt
    24901 gtacaaaaac ccaccgataa aaccagtgtt atagtagttc attttctggc tgtaaggtat
    24961 ccatagtgcc gttttattat atgcttttcg caacaaatta tcgggattgt tgaaattact
    25021 gatataccct agcagtccaa ttgccacaag cagtgtcgtt aatcggatca attggaatcg
    25081 ccttcctttt ttcaggcttc gaaacatgta ccagcaaaac aatcctatca cggttcctgc
    25141 ggcagctaag atcactgtaa acagcatagt gccggtcata tctttcagca atccgatttc
    25201 agtgatcatc ttcaaatcgt ctggataaat cggttcttgc cgataactca ttttcatata
    25261 atttgcaaaa cctaatacac cgatggctac gacatacgta agggaaccta aagggacaga
    25321 tcctgctagt gcaatcagaa aaataagaat aatcagcaag acgagacagg ctaagaagaa
    25381 tttttccgta tgccaagaaa aagcaaattt caacgcaagg tctacggata gatcattttg
    25441 acaccactgc aaaaataaat tactaaaaac aacaagaaaa aagatactga tgattgctgc
    25501 tgttcctttt aaaatctttt tctgtttggt cgaaagtctg cttatatatc ttttcaaatg
    25561 cttgctcctt catttctatc aataaaagaa tgttgcttct cttattttag ttgtacgaaa
    25621 ttcgaaagta aagaacggaa agaagaatta atgaaatcca aatattctct ttctttatgg
    25681 cctttctggt ttctttaata agcggacctt tcgtactcca gaaatttttt ctagctgttg
    25741 tttcacttga ttgagctgtt caaaatcagt actgtctatg tcaatcaata tgtagacatg
    25801 ctcattctta tggttacgcg atatatctgc gatcgctagc tcgttctcat tcagcagaga
    25861 aaatatctct gctaatatat tggttttctt ttgataaaat atagtgaatc gaaacggcgc
    25921 ttggaaaaac attcgtgcgg atggataatt gaccgcttct ctaaccgtac cgaacaaaag
    25981 ataatctttt aaggcttcaa ctgccaaacg tccgctgtct gccaaagctt cctgagtcga
    26041 tcccctagat gaggcaacat aagtatacgg tcattttcta agaactcttc ttctggaaaa
    26101 tcagtcaagt atttagccag atgttgtttt tccaatgctt gcataacagc atatttatca
    26161 acaatttcac ttcttcctac attgataagt acagcatctt ttttcatttt ttctatattc
    26221 gcctgatcga tcaatccttt tgtatcttct gtcaacggca gcatgactac gatgtaatcc
    26281 gaggtagaaa gaactgtttc taaatctgct tgccgaacat aatccaactg ggcatcgcgg
    26341 atagaataac ccaagacatc catccctaag ctatagcaac ttagcgctac ttttgtgcct
    26401 atcgctccaa gaccaagaag accgatggtt ttgccttgca attctcgtcc cacataagca
    26461 cttcttttgt tttctgcctg ttctaaaata ttaggacctg ttagtgtttg taccatacga
    26521 cttgcttcga ttatcggacg ggaagataag agcaaacagc acaaaacaag ctcttttact
    26581 gcattagcat ttacacctgg tgtattcatg acgatcgtcc cgttttcaga agctttttca
    26641 acgttgatcg tgttcacccc aacaccagct cttgaaattg ctagcaaatc tggtgtaagc
    26701 cattcatctt tcactttcgt actacgaata actattcctt cggctggcaa atcatctatt
    26761 attttgaaat attccccttg taacttttct atctcttgtt ctggaatagc atcaatcaaa
    26821 tgaacgtgat acataaaacc ccctccttac taacaagtat ctcgaaattt ctgcaattag
    26881 acaactgttt accatttgac ttcctagaag caaaaagagc agttagagca gagtaggctg
    26941 agtgataaaa ataaggctat gacaagttac tgccataagc cttattttcg agagcaaaat
    27001 attgagggtg agatatcttg ataaatggtg ttccgttagc tgacattgat aataagacta
    27061 aatttcaaaa aagttgaaaa gcaaatttcg tcaatctctt cttatttctt aaaactaagt
    27121 ggctttttca caacctcttt ttatctaata accactgttt ccaactcaac taattttgcc
    27181 cacgtttcaa tttgatctgt tgttagattt agcgagacaa cggtatgatg accgccacct
    27241 gcctgaatcc atgaatggac accttcatgg aaattcggtt tagttttcca taacacacgt
    27301 gcaacaggta aattaggtgc ttcagtcgtt ggttcaaagg cttctacttc attgatcagc
    27361 agtttataat gcgtaccgaa atctgccatc gatacgacta ccccttctcc tgctttccca
    27421 tcaaatacaa gacgcgccgg atcttctcta tctcccattg ataaaggaga aacaacgatt
    27481 cttggtttcg tcgcagcaag cgtcggatct acttccatca tatgggattc caagattgct
    27541 tcttggccac tagctagctc gtaagtataa tcttccatga atcctgtatc tttccctcga
    27601 gccatgattt tcagcaaccg atcaatcgct gcagttttcc agtcgccttc cccagcaaaa
    27661 ccgtatcctt ctgccatcaa acgttgtaca gctagtccag gtagttgttt cattccatac
    27721 aggtcttcaa aattagtcgt aaaagcgtta tagcctcctt cttctaagaa acggcgaata
    27781 ccgatttcct gctgagcttg tacttttaca tgttcctccc atgtcttttc ttcataatca
    27841 ccatagtcga aggtatacaa ttccttgtac tcttcaaata attgatcgac ttcttcgtct
    27901 gatacgcgat cgattacttg tacaagatct ccgataccaa aataatcaac agtccaacca
    27961 aattgaatct gtgcttccac tttgtctcct tctgtgacac ccacgtttct catgttgtca
    28021 ccaaaacgag ccacttttat tccaaaactt tcattatagg ctactgcaac atccatccaa
    28081 tcagcaatct ctttttggat ctcaggtcgt ttccagtagc caacgacgat tttattttgt
    28141 ttatttaatc gagcattgat gaacccatat tcgcggtcac catgagcgga ttgatttaaa
    28201 ttcatgaaat ccatatcgat cgttttccaa ggaatgcttt cgttatattg cgtagctaaa
    28261 tgaagcaatg gtttttgtag aagctttgtc ccgcggatcc acatttttgc tggtgagaaa
    28321 gtatgcatcc aagtgatcac acctgctact tcttctcgat aattgacctc tttcatgatt
    28381 tttgtgatcg tatcagcagt aactgctaat tcttgaaggc ggattggata aggcaattgg
    28441 ccattttcat tcagttcatc gaccatttct tgtgcatgtt tctttacttc tcgcagtgct
    28501 tcttccccat ataaatgctg tgatcctaca acaaaccaaa attctttttc tccaatattc
    28561 aacataatta gccactcctt ttatttgttt tgtccgtaat acgcattttt tccgtgtttt
    28621 cgtaaataat gtttatctaa aatccgttga ggcagctctt ctgaaaatga attgagctgt
    28681 ctggtaaata aattcatctt gcatacttca tctagtacga ctgcgttcat taccgcactt
    28741 tgcgcatctt tcccccaagt aaatggaccg tgtccgtgta aaagtacccc tggaattgct
    28801 aagggatcaa ttttgcgttc tttgaatgtt tctatgataa cttttcctgt ttcatattcg
    28861 tacccgctgt cgatttcctg ttgtgtcaaa aatcgagcac aaggtaccga tccataaaaa
    28921 gtatctgcat gcgtcgtccc catcgcagga acatccaatc ctgcttgtgc ccagatcgtc
    28981 gcccatgtcg agtgagtatg gacgattcct ccgatttgag gaaatgaacg atataaaaca
    29041 gcatgagtag gcgtatccga agagggattg agtttacctt caatgatatg attgtccaag
    29101 tccacaacga ccatgtcgtc tgctgtcatc tcttcataac tgactccgct aggtttgata
    29161 acgaaatatc gtgtttcagg atcaaaagcg ctcacatttc cccatgtata tttgacgagt
    29221 ccgtgtttag gcaagtcaag atttgcttgg tatacttctt ctttcagttg ttccagcatt
    29281 ttctatcact cctttttctt gcaattgatg aatcgctgtt ttttcaatgt cgatcccctc
    29341 acggtaacgt ctcataaata tctgatagcc tgctatttct tctgctgaag gtgtgatggt
    29401 gattccttgg tcttgtccaa acacgtcttc tgccaagaag ttttctaaag acttcccatg
    29461 tcgatccatc atataggctg ctaataacgc gattccccac gctccacctt ctccggctgt
    29521 gtccatcact gtaactggtg cctccatagc agatgctaga atccgctgtc caacttctgg
    29581 tgttttaaaa atccccccat gccccactaa acgatcaatt tggacattct cagatttcaa
    29641 tatatccatt ccgattctca ttgcaccaaa agcactggct aaatggatcc gcatgaaatt
    29701 cgctaaatca aaacggctac ctggtgtacg tacaaataat ggccttcctt catttactcc
    29761 tgtaatgttt tcaccggaaa aataaccata agaaagtaat cccccgcaat ccgtatcgcc
    29821 ttctagcgct ttgttgaata ataccgtgaa taatttatct ctgcttatct ccatacccaa
    29881 agaattggta aattcttcaa aaatactgac ccaagcattg atatccgaag agcagttatt
    29941 cgtatgaacc atgcctacta gttctcctga aggtgtagtg accatgtcga tttccggatg
    30001 gacattttct agttcttttt ctaatacgat catagcgaag gcggaagtac cagccgaaat
    30061 gttccctgtc cctttggcta cactgttcgt agcgaccata ccggttcctg catccccttc
    30121 tggcggacaa accgggattc ctggtctcaa atttcccgta gggtcgatca gataagcccc
    30181 tgattcgctt aacttgccag cttcttctcc tgctactaga actttaggca aaatgtgatc
    30241 caagtcaatc gctacgtgcg ctttttcgat taactgacca aaaatatttt tcatttcttg
    30301 atggtaggtt ttcgttgaag gatcgatagg aaacatgcct gatgcatctc ctacgcctag
    30361 tactttctca ccagtaagct gccaatggac ataacctgca agcgttgtca aaaaattaat
    30421 ttctttcaca tgttcttctt tattcaaaat agattgatac aaatgagcga tactccatct
    30481 ctgcggaata ttgtattgaa atttttccgt taatattcgt tctgcttctt cagtgattgt
    30541 atttcgccaa gtacgaaatg gtactagtaa ctgttccttg ctatcaaatg ctagataacc
    30601 gtgcatcatt gcactgaacc caatcgaccc gattgtcgta agattctcgc catagatttc
    30661 gaatacttct gctgccattt ttcggtaact gacgcgcagt cctttccaaa tatcttctaa
    30721 tgaataggtc cagataccat tttctagttt gttttcccac tcataactac cagcggcaat
    30781 aggagtaaaa gatgaatcaa tcaatacagc tttgatccta gttgatccaa actcaatccc
    30841 taaagatgta cgaccatcta tgacatctaa catactttgc gcatttactt ccttcataat
    30901 gatttaactc cctccttctc gtcaaacctg atcaaaccgt ctttttagta gcgcttacaa
    30961 tgattattgt atacttttgt acgtacatgt caagcagtta tttacaagaa tacaataaaa
    31021 aacacttgtt ttgtacgtac ataagttata aaatgaaatt gcttacaaaa tctgatcaac
    31081 cgccttctcg aaattctaga aggagagaat acttatgaaa aaagaaaaaa aagtttcctc
    31141 atcgtttatt tatttttttg gcgcatttgg cggcattcta tttggatatg atattggtgt
    31201 tatgaccggc gccttacctt ttttacagca tgattggggt ttggcaggta aagcttcttt
    31261 gataggatgg atcacgtctt cggtcatgtt aggtgcgatc cttggcggct ctttatctgg
    31321 tcttctttct gataagcttg gacgacgaaa aatgattctt ctttctgcac ttatttttat
    31381 ggctggttct gtcctttctg catctgcccc tcacaatggt tcttattttc tgattgcagc
    31441 aaggattctt ctaggtttag ctgttggcgc tgcatctgcc ctagttcctg cttatatgtc
    31501 tgaaatggct cctgctagac taaggggaag attgtcaggg atcaatcaag tgatgattgc
    31561 ttctggcatg ctcctttcct atgttgcaga ttatttgcta aaagggttgc ctgaaacgat
    31621 ggcttggcgc gtgatgttag ggctcgctgc tgtccccgcg ctgattttat ttttcggtgt
    31681 tttagctcta ccagagtcac cgcgttttct tatgcaatct ggccgcttgg aagaggcaaa
    31741 aagagtattg aactatattc ggacaccaaa agaagctgaa caggaattcg aacaaatcca
    31801 gctgaatgtc aaacaagaaa agacaaccgg aacttcttgg catactttgt ttttagaaaa
    31861 atatcgttcc ttagtcattg ccggtattgg tgtagcagta ttccagcaat ttcaaggagc
    31921 taatgctatt ttttactata ttccgctgat cgtagaaaaa gcgactgggc atgcagcgag
    31981 cgatgcattg atgtggccga tcatccaagg aatcatttta gttgctggtt ctttgctctt
    32041 tttggttatt gcagacaagt tcaatcggcg aactttattg aaaataggcg gtagcgtgat
    32101 gggactttct ttcatcctac cagctgtttt aggaactgta ctggatgctc atacaaattc
    32161 gttgttgatt ctcttgtttt tatgtattta tgtcgcattt tattcttgca catgggctcc
    32221 actgacatgg gtaatcgtag gcgaaatatt cccactagct gtccgcgggc gagcatctgg
    32281 cctagcctct tcttttaatt ggattggttc tttcctagtt ggcttgcttt ttcctgtgat
    32341 gactgcttct atgtctcaag aaatcgtttt cggcattttt gggatcatct gctttttagg
    32401 ggttttattt attcaagaga tcgtaccaga aactcgtggg aaatctttag aggaaatcga
    32461 acaaagtgca tctaaaaaaa cttatcctaa acgtatttct atccatcatt cttaaagagg
    32521 ttgtgacaga agtggtcagc ttcaagaaat aagaaggaac cgcttagttt ccagtcacaa
    32581 ggaacaattt caaaaaacag cttctcatgt ttgatgaata ttgtgacttg tgaccagttg
    32641 gtaggagaac accgtttagt ggagaggtgg tgaaagaaac gttcagcctt gagcgatgac
    32701 aaaagctctc acaaacattg cgacgagtaa ccgtaggagc aataagaggc ggtaaaaacc
    32761 cgtctcttat ttttcgtttt ctttcactgt ttcccgttca actagtacag gttcatagag
    32821 aatatttggc tgtgcctgtc ctttttcaat cgttgtgacg atccaccgtg cggcatcttg
    32881 tcccaacttt tcttttggat gcgtcaatgt tgtcaatttt ttattcccta aatggcttaa
    32941 agaagaatcg tctgttccta cgatcgatag gtcatctgga atatgatagc cattttctgt
    33001 caacttatct agcattaaac tagctatttg atcgttatag caaatgatgc catctaaagc
    33061 ttgttcttct ttcaaccgtt gtactgtttt ttcagcgatt gtttctcgtg tttccgtggt
    33121 gaaagtaatg atatcttccg tcaaaaaacg cacattatac tgttcacacg cgctgataaa
    33181 acctttcatc ctgtattttc cttgaagatc gtcgattttt gtgatgaaca acagacgctg
    33241 atgtcctttt tttatcaaat attctgctgc cataaaaccg actttcacat catctaaaca
    33301 aatataagag gcatttaact cttcataagc agcattgatc atcaccatcg gaatgccttg
    33361 ttctctcaaa gcaacataaa gtgctaagtt tgggttatac tgattacttt tcgtaggctc
    33421 aacgatcaaa ccatctaccc cataagcaat cattttcttc aaacactctt tctccaattc
    33481 gtgatcgtta ttcgtactgg ctaaaagcaa tgaataccca gattctcgta attcattttc
    33541 gattcctcgg ataatcgacg gaaaaatata atcagagaga tatgtggtga tgacaccgat
    33601 cgtttttgtt ctgttcaatg gtgttttttc tttcttatag ctgtcatcta catatgtgcc
    33661 cgaccctttt tctttacgca gatagccctc atttaccaac acagcgattg cttgccgaat
    33721 cgtatgtctg ctgacgtcat atatttcttg cagcttgaat tctgagggaa tggttgtatt
    33781 tacttcgtat tcctcacgaa ggatcttttg ttttatctct tctgcaatat cttgatattt
    33841 tgccatttgc cttgcccctt tttatttgta cgtacatata tcataacgag tctgcaaggt
    33901 ttattcaagc aaaaatcaag gtttcttgtg ctcataaggc atacggaaca ggttcaccaa
    33961 attctggaaa tccttgctca ttccacgtaa agcattgcgc gttcgcgtgc cgattaggat
    34021 tgtctaacgg atcccctttt tggttcttct ctggtcgagc atgatagatc aaagtatcaa
    34081 agcacccgtc ttctgaaacc gtaaacgagt tgtgaccagg accaaattgc ttattttttt
    34141 cagaagaaac gaatactggc tccgataatt tatgccatga ttcttttaat aaaagattct
    34201 tgtgctcatc agcccacagc aggcccatgg catagttttc atctgttgca ctaccagaat
    34261 acgtaatgaa tattttccca ttgcgagtaa ggactgctgg accttcattt actgaaaaac
    34321 cccgtttttc ccattcatac tcaggtatag aaaggaggat ctgcttcccc tttaacgtcc
    34381 aaggattatc catttcggat agataaagat tagaattccc agtaatttca ggatcttgtt
    34441 gggcccacac ataatatagc tttccttgag aagagaaagt tgttccgtct aaactgaaag
    34501 tttccctttt cgtttttatt tgtccctttt ctatccaatc tgtattcatt ggatctatat
    34561 gctgattttc aagtacgaac atgcggtgat gatgagattc atcacgtata gttttatgat
    34621 tgcttgcagc aaagtaaata taccattttc cattgatata atggacttca ggtgcccaga
    34681 tcaattcact catcggtcct tcttcatgag cctgccatac aatcaggctt gcagcatgtt
    34741 ctaattcatt cagtgacttt gcccttctta attcaatcac ttggtagcct ggaaccgatc
    34801 ctgtaaaata ataataacca tctgtatgtt tatataccca aggatctgcc cgttgccgaa
    34861 cgataggatt attataaagt gtcttcatca taacaaattt cctcacattt tctttattct
    34921 aattacattc caagagtatt tttctatccc tacatttaat tcgtcatgtt tttctattga
    34981 aaaatagttt atttcaggtt ttattttttc tggttcttca cgtgtatttt tgtccaacag
    35041 atcgctactg gttaatgtgt agtgctctcc ttgatctagc aaattgaaat catgttcaat
    35101 cgttatcttt tgttgttcat ttgtgcgatt gatcaagaaa atagtcagct gattgccgtt
    35161 atctacgatc acactgtcca cataaggaac attttttatc ttttcacaat cataaacagg
    35221 gcaaatgatt tgttgtttca atacttgacc tttagcatat ttcgataagt gttgcatcac
    35281 ataataagtt ggattcctcc atgcttgtcc tttagcatct gtcagtatta aaggtattgt
    35341 gtttactaac aatgattgac aactaatctt tacgcgatct gcgtgtcgta aaatagccag
    35401 ccctaatgaa ccaaataata atgtatcttc catagtaaag taacaccaat cgatgggact
    35461 accgacttca aaatccctat gtcttttttc cggttgggca tgtacattcc attcatcaaa
    35521 cgctagatac attgttttct cacttctcaa atatcctttt acataatcac aagtagcaac
    35581 gatttcttca atttgacgat cgaatcgata agcttttgca aggtatgtcg ctgtatcatc
    35641 acgctcattt ttaggaggtt tagtcaagtc ttcttcatca tacttatcaa tatagttatg
    35701 caaagctaaa taatctacat tctcataagc ttcttctaat gcgatacgat cccattcagg
    35761 gtacgatggt aaacgtggcg tagagcttcc aactaagatt gtttcaattg aatcatccac
    35821 taacttcatg acttttgagg cttcatttgc taacctacca tattcataag cactcttagc
    35881 ccccacttgc catggaccgt ctagctcatt ccctaaacac cataatttaa cgccaaaagg
    35941 ctttttttgc ccatttttta ttcttaaatc actccagaag gttccttttg gaaaattaca
    36001 atattctact aaattttttg cttcatcaat tccttttgtt cctaaattca ccgtgaatat
    36061 cggtttcgct tgtacatgac tgatccattt aaaaaattcg tggagtccaa aagaatttgg
    36121 ttctatacct cgccatgcta aatcaatttt ttttggtcgt tgttctttcc tacctacagt
    36181 atccacccaa tcataaccag atgtaaagtt tcctccagga tagcgaatga cacctaattc
    36241 taattccttt acataagaga ggacatcttg tctaaacccg tcaccatcag ctgtttcatg
    36301 gcccggttca taaatccctg tatagacgac acttcccata tgctcaacaa atgagccaaa
    36361 cagccgagaa ctgatctctc ctaccgtaaa atcctctctg atttttactt tcacttcttc
    36421 gtccaacagc tttccctacc tttcatttcc tatagtcaag ataatagata caagtttcca
    36481 tacgccacca gcaaagataa aaataaaagg agggaaaaga aatatgagca gtacaaatcc
    36541 taataacaat acgcaacaaa gaatatttct caaaaaatca aaaaatactt tccatgcact
    36601 ttgttcaaaa atttgtaaaa atgtgatatc tttgttttca gaaatcacat atatccagga
    36661 taaaagaaac atggaataaa taagtaaaac tacatatcca ctaattgaaa gtacgattgt
    36721 ctgttcttct aataattgat taacaagaaa ataaataatg atcgttaaca gactaaggaa
    36781 caagttctta ccaaaatgtt gctttattcc ttgaaagaaa aaataaatga tatttcctga
    36841 tccctcacct ttccattgcg ccattacata aaaaacagca catgtagcag gcacgatagt
    36901 aataacaggc aaacacacga ttacccagag aatacctaat aagatgtaat cagaaaccac
    36961 aatcaactgt ccataaagtt tagtctcaac tattttcatc ttcctcactc tttcattcca
    37021 gacatagctg ttccttgaac aaaatatttc tgtgcaaaaa tataaaccac tagtaatggc
    37081 agcattgaaa taaccgttcc agccattaca ggtccataaa aagaaatgtt ctgccctgta
    37141 aataaagcta accctgctgg taaagttctc atttttgaac tacttgtcaa tatcaatgga
    37201 taaagcaagt cattccaact attcatcagc gtgaaaatcc ctaatgataa aagagtaggt
    37261 ttgctcaatg gcaacatgat tttcaaaaaa atattaaact cacttaatcc atcaatgcgt
    37321 gctgcctctt ccaaagaatc tggtaaagta ataaaaaagg aacgcatcat gaagatacca
    37381 aatgcagttg tcatacgagg aatgatcaaa cctgcataag tatcaagcat gccaagcaaa
    37441 ttgacttcaa taaatagagg aatcatgaag acttggaatg ggatcatcat tgtcagcaaa
    37501 acgaaataga acaaaactga ttttcctttg aaacgcatac gagcaaatgc ataccccgca
    37561 agagaatcaa agaatacaga agtgatcact gtccctccag caaaaatcac tgtatttttg
    37621 atgtaatcta ataaagggat cgttgtccaa accttaatat agttatctag ggtatagcta
    37681 tctgcaaata ttctaggcgg aaatgagata atgtcttttt cgtgtttaaa agaagaaata
    37741 atcagccaca aaaaaggaaa cgcaataacc aacaatataa ctagcatact aatcgttcga
    37801 atgatttttt taccactaac tgcttttcga ctattcacag taactgtaat cgagctttct
    37861 ggtgccatac aggtttccct cctatttcat ttgtttttct ttccgtgaca tgtacacatt
    37921 agtggagata gctatagttg caacaatgat aaacaaaacc gaagacagtg ctgatgcata
    37981 gcccaattca taaggtgctg taaatcctcg actgtaaata tattgaacag ctgtttctgt
    38041 cttaaattgt gggccaccag cggttgcaac atatacttgg tcaaacactt gcatcgaacc
    38101 aatcagattc gttaaaatac aaaaacctaa ggagggaacg atctgaggta gcgtaatgtt
    38161 aaaaaactgc tgtcgatttg ttgcaccatc catttgagca gattcataca aagagggaga
    38221 gattccctga atagcagtca agagaatcgt catagtaaca ccaaaattct tccaaacagt
    38281 catgatggct accgtcggca tagcaagggt tggatcgcga aaaaattgag ggatcggcaa
    38341 gccgacttgt gaaaacaagt aaggaatcgc ccctacattc ggatcaagca acatagaaaa
    38401 aataatcgca attgacgtca atgaacaaat aactggaatg taaaatgtcg aacgacaaaa
    38461 acgattgaat ctggtatttt tagataatgc tactgctgct aataaaccaa cgataatttg
    38521 tgttggcgtt tcgagtacgg tgaaatataa agaatgaatc aacgaattaa ctgctcgttc
    38581 atcactaaat atacgtgtga aattttcaaa acttacaaaa tttcttgatg taaagaaaat
    38641 attcaaatct gtaaaactaa tagcaaatgt tcctaaaagc ggtatgacag taaataataa
    38701 taaaactaaa aaagatggag ttaaaaacaa atacgcagca cgatcaggac gattccaata
    38761 tttggtgaat ccgttcatat agtccacctc atctttcatt ttgataaaat atacaatctt
    38821 ggtttaaacg aatctgaata agatatccga acaatcgctg tgatttttgc aataacacct
    38881 atctgcaaat actaggtcga tcattcagat atctgtatct tgccaacagt ctatcctgta
    38941 taaagttctg tactattttt gcagtaattg attgatggca tcatctgcat cgttcatgag
    39001 tgtctcagga tcttctcctg ctaataattt ttcaatcagt ggattgatga tatcgttatt
    39061 gatcgcagag atttgggtaa aacctggtaa atatggctct gcatagtcca tttgtttcga
    39121 taattcagat acaataggat cattttttac ttcttcatct tttgctactg aatccaagta
    39181 ggcaggaaaa ccattttcga tactccattt tttaccaatt tcagttgtat tccaatattt
    39241 caaaaactca tagacagctt cttttttatt attatctgta cttgccggaa tggcatatcc
    39301 tacgccatct aaaatagctg tttttttgtc tgaatcactc ccttgtggta aggtagtcac
    39361 attataatca atctcgtttg ctttcaatcc gttatttaac catggcccat tgatttcaat
    39421 ggctagttga ccagcattca ttaaattgtc catttcggcg ccagaaatcg attctggacc
    39481 agtcttgtct acatggatca ttttttggat cgcttttaac gttttaaggt tctcaggaga
    39541 cgcaaattca gacttagtat aatcatcgtt cactaattta ccgccatttg ctaacatcca
    39601 gttatataac ggtgcagttc catccttatt gaagacaaaa cctgatacat ttttactact
    39661 atcagttaat tttggtgcca tttctgtcaa ttcttcccaa gtagtaggtg gttgttcagt
    39721 atctaatcca gcttgtttaa acaaatcatt attccaatac atatacatgc cttgtacctg
    39781 catggggaca aaatactgtt taccttctac ttcacctaat tcaatagccg tttctttaaa
    39841 atcagattta tctactccat catattccca gaaatcgtct aatggttgta ccgctccgtt
    39901 ttctacatac tgtgcaaaat ctggataatt taatgcaata aagtcaggtg cttttttaga
    39961 agaaatcgct gtaggaagtt tttcattcaa attggcccaa gtcatgatat ccatttcaat
    40021 tttgatgttt ttatcatttg ttttattaaa ttcattaacg atttcttgca atatttcacc
    40081 atcagatgca gtaaacccat tccaaaaagt caaagtcact tctttatcag aatcagattc
    40141 tgctgaactt ccgccaccgc aagctcctaa aatacctgtt gcagctacca acaaagagcc
    40201 aattaaccat tttttacctt tcattttttg ttcctccttc atctgataaa cgacatctga
    40261 ccacattcca agatagcggt ttcaataaac aattgatttt cccatcttca gcttttacat
    40321 ttgttgaatg acttggtttt acttgttcac ttttaggaag attagttgct tttgtatcaa
    40381 atccactcat ttcagaaaaa tcaatcactc cctctaatga taattcagac aagtgaacag
    40441 ttagattcat ttcttcttct ccacggttaa ctgcaaatat gacaagttct ttgtcattta
    40501 ttacggctat actttctaaa tacggcactt cttcaaaatc ttccgtcgca tagtttgctg
    40561 aacgaatttg tgggcttaac acctctcctt gtccatacac agcagcttgc ataaatggat
    40621 aatagatcgt ctgtctccaa gcttctccgt ttgtttctgt cctgattggt gcaataacat
    40681 ttaccaattg agcaagacaa gcaatcttta ctctatctgc atgtttcaat aaagtgatta
    40741 atagacagcc taatagtaat gcatcttcaa aattatagtt atcttctagt aaaggaggag
    40801 caatctgcca aggcgctgct tgctcatctt gttccgctga atgataccaa atattccatt
    40861 catcaaagga caggttgatc tgcttcttac tatgtttctt cgccttgact gtatcgcaca
    40921 tcgctgtcac ttcagaaatg aaccgatcca tgtctaagga acgtgcaaga taatgagaaa
    40981 gatcattttt ttggttgcca taatattgat gcagagacaa ataatctatt tgatcgtacg
    41041 cttgatcaag aactgttaac tcccaatcac caaatgtagg catttgccga ttcgaacttc
    41101 cgcataaaac cacctcaatt gaatcatcta cgagtttcat cgccttagcc gtttcctctg
    41161 ctaaccgtcc atattcttcc gctgttttat gtccaatctg ccaaggaccg tccatttcat
    41221 tccctaaaca ccatgtctta attccaaaag ggctctcttg accattcgaa cgccttaaat
    41281 cactccaata agttccttta ggaaaattac aatattcgac taaattacga gctgcatcta
    41341 ctccacgagt acccagattg acagccatat tgatttcggc ccccacatgc tgacaccagt
    41401 tataaaattc atgaatccca acttcattcg tttcaattgt ccgccaagct aaatccaacc
    41461 ttctaggtct atttgctatg ggcccaatac tgtcttccca acgaaatcct gacacaaaat
    41521 ttccaccagg atatctaatc atgggaatgt tcaattcttt taccaagtga atgacgtctt
    41581 ggcgaaatcc acttttatta gctgatggat gatctggctg ataaatccct tcatatactg
    41641 ctcgtcccat atgttcaata aatgaactgt acaaccgcgg atcaactttg cctacacgct
    41701 tgacactact aatcaacatt gtggcttcca taactgacct cctttaattt atacatgaat
    41761 aatacacctc gttgaaagcg ttg
//
LOCUS       DAXWLF010000004        39901 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXWLF010000004 DAXWLF010000000
VERSION     DAXWLF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.004"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            20..901
                     /locus_tag="ACGE4U_000135"
     CDS             20..901
                     /locus_tag="ACGE4U_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8392332.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            914..2524
                     /locus_tag="ACGE4U_000136"
     CDS             914..2524
                     /locus_tag="ACGE4U_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8392333.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAKGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2601..3260)
                     /locus_tag="ACGE4U_000137"
     CDS             complement(2601..3260)
                     /locus_tag="ACGE4U_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8392334.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3543..4208)
                     /locus_tag="ACGE4U_000138"
     CDS             complement(3543..4208)
                     /locus_tag="ACGE4U_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFF8392335.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4355..4960)
                     /gene="dhaL"
                     /locus_tag="ACGE4U_000139"
     CDS             complement(4355..4960)
                     /gene="dhaL"
                     /locus_tag="ACGE4U_000139"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFF8392336.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4988..5998)
                     /gene="dhaK"
                     /locus_tag="ACGE4U_000140"
     CDS             complement(4988..5998)
                     /gene="dhaK"
                     /locus_tag="ACGE4U_000140"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFF8392337.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5973..6368)
                     /gene="dhaM"
                     /locus_tag="ACGE4U_000141"
     CDS             complement(5973..6368)
                     /gene="dhaM"
                     /locus_tag="ACGE4U_000141"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFF8392338.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6522..6842
                     /locus_tag="ACGE4U_000142"
     CDS             6522..6842
                     /locus_tag="ACGE4U_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFF8392339.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6954..7811)
                     /locus_tag="ACGE4U_000143"
     CDS             complement(6954..7811)
                     /locus_tag="ACGE4U_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8392340.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7919..9223)
                     /locus_tag="ACGE4U_000144"
     CDS             complement(7919..9223)
                     /locus_tag="ACGE4U_000144"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8392341.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9220..9729)
                     /locus_tag="ACGE4U_000145"
     CDS             complement(9220..9729)
                     /locus_tag="ACGE4U_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392342.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9904..12039)
                     /locus_tag="ACGE4U_000146"
     CDS             complement(9904..12039)
                     /locus_tag="ACGE4U_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFF8392343.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12122..13777)
                     /locus_tag="ACGE4U_000147"
     CDS             complement(12122..13777)
                     /locus_tag="ACGE4U_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8392344.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13779..14093)
                     /locus_tag="ACGE4U_000148"
     CDS             complement(13779..14093)
                     /locus_tag="ACGE4U_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8392345.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14263..14523
                     /locus_tag="ACGE4U_000149"
     CDS             14263..14523
                     /locus_tag="ACGE4U_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFF8392346.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14537..14851
                     /locus_tag="ACGE4U_000150"
     CDS             14537..14851
                     /locus_tag="ACGE4U_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFF8392347.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15581..16135)
                     /locus_tag="ACGE4U_000151"
     CDS             complement(15581..16135)
                     /locus_tag="ACGE4U_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFF8392348.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16261..18190)
                     /locus_tag="ACGE4U_000152"
                     /pseudo
     CDS             complement(16261..18190)
                     /locus_tag="ACGE4U_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18496..19212)
                     /locus_tag="ACGE4U_000153"
     CDS             complement(18496..19212)
                     /locus_tag="ACGE4U_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFF8392349.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19213..21036)
                     /gene="glpO"
                     /locus_tag="ACGE4U_000154"
     CDS             complement(19213..21036)
                     /gene="glpO"
                     /locus_tag="ACGE4U_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFF8392350.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21050..22546)
                     /gene="glpK"
                     /locus_tag="ACGE4U_000155"
     CDS             complement(21050..22546)
                     /gene="glpK"
                     /locus_tag="ACGE4U_000155"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFF8392351.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22682..24142)
                     /locus_tag="ACGE4U_000156"
     CDS             complement(22682..24142)
                     /locus_tag="ACGE4U_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8392352.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24132..25427)
                     /locus_tag="ACGE4U_000157"
     CDS             complement(24132..25427)
                     /locus_tag="ACGE4U_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8392353.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25759..26436)
                     /locus_tag="ACGE4U_000158"
     CDS             complement(25759..26436)
                     /locus_tag="ACGE4U_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFF8392354.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26466..27557)
                     /locus_tag="ACGE4U_000159"
     CDS             complement(26466..27557)
                     /locus_tag="ACGE4U_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFF8392355.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27658..28092)
                     /locus_tag="ACGE4U_000160"
     CDS             complement(27658..28092)
                     /locus_tag="ACGE4U_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFF8392356.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28676..30133)
                     /locus_tag="ACGE4U_000161"
     CDS             complement(28676..30133)
                     /locus_tag="ACGE4U_000161"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8392357.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30130..32025)
                     /locus_tag="ACGE4U_000162"
     CDS             complement(30130..32025)
                     /locus_tag="ACGE4U_000162"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8392358.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32083..33858)
                     /locus_tag="ACGE4U_000163"
     CDS             complement(32083..33858)
                     /locus_tag="ACGE4U_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8392359.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33908..35404)
                     /locus_tag="ACGE4U_000164"
     CDS             complement(33908..35404)
                     /locus_tag="ACGE4U_000164"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8392360.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35598..37220)
                     /locus_tag="ACGE4U_000165"
     CDS             complement(35598..37220)
                     /locus_tag="ACGE4U_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFF8392361.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37548..38861)
                     /locus_tag="ACGE4U_000166"
     CDS             complement(37548..38861)
                     /locus_tag="ACGE4U_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFF8392362.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39106..39330)
                     /locus_tag="ACGE4U_000167"
     CDS             complement(39106..39330)
                     /locus_tag="ACGE4U_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392363.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39350..39772)
                     /locus_tag="ACGE4U_000168"
     CDS             complement(39350..39772)
                     /locus_tag="ACGE4U_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8392364.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
BASE COUNT        11261 a         8533 c         6830 g        13277 t
ORIGIN      
        1 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
       61 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
      121 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
      181 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
      241 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
      301 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
      361 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
      421 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
      481 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
      541 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
      601 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca ctcgatgaag aaggaacact
      661 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
      721 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
      781 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
      841 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
      901 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
      961 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
     1021 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
     1081 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
     1141 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
     1201 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
     1261 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
     1321 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
     1381 agcggattcg atcactgcaa aaggtgcttg gctttttggg ctatctgtag gtagtatggg
     1441 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
     1501 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
     1561 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
     1621 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
     1681 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
     1741 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
     1801 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
     1861 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
     1921 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtag tcttgatttg
     1981 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
     2041 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
     2101 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
     2161 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
     2221 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
     2281 atggcttcct cgaatcggta aacttgctta tatctactta gcttactcct ttgcactcaa
     2341 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
     2401 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
     2461 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
     2521 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
     2581 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
     2641 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
     2701 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
     2761 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
     2821 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
     2881 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
     2941 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
     3001 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
     3061 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
     3121 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
     3181 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
     3241 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
     3301 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
     3361 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
     3421 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
     3481 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
     3541 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
     3601 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
     3661 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
     3721 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
     3781 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
     3841 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
     3901 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
     3961 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
     4021 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
     4081 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
     4141 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
     4201 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
     4261 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
     4321 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
     4381 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
     4441 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
     4501 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
     4561 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
     4621 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
     4681 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
     4741 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
     4801 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
     4861 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
     4921 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
     4981 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
     5041 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
     5101 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
     5161 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
     5221 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
     5281 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
     5341 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
     5401 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
     5461 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
     5521 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
     5581 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
     5641 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatcggctt
     5701 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
     5761 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
     5821 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
     5881 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
     5941 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
     6001 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
     6061 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
     6121 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
     6181 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
     6241 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
     6301 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
     6361 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
     6421 aatgaacaat tcaagtatat aataaatttc taattttggg aaaatatgtg tagtgcttct
     6481 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
     6541 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
     6601 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
     6661 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
     6721 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
     6781 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
     6841 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
     6901 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
     6961 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
     7021 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
     7081 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
     7141 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
     7201 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
     7261 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
     7321 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
     7381 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
     7441 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
     7501 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
     7561 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
     7621 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
     7681 ccagaagagc ttatggttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
     7741 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
     7801 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
     7861 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
     7921 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
     7981 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
     8041 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
     8101 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
     8161 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
     8221 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
     8281 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
     8341 gccagacttc tgttccacct tttgcccaac gttttctgtg tcgatatagc atcttcaacg
     8401 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
     8461 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
     8521 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
     8581 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
     8641 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
     8701 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
     8761 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
     8821 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
     8881 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
     8941 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tattcgatca
     9001 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
     9061 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
     9121 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaaatacata atagacaaac
     9181 aagtatctaa gaataaatta cctgtatggg tgattgagat catttcagtt ccaccccatt
     9241 ttctttatac aaatctgcaa tgaatgtcgt atctagattt ttctcttcgg agacagaata
     9301 aaaacggacc gtttctctct cttcttttgt tccaaaacgt tccgtataca cttcttcaag
     9361 taattcttgt ctttttttca atttttcatc ttcatattca aaagatttat ttactttttt
     9421 gtatagattt cgattgttcc acctagtcaa tacaaatgag gtaatcagga aactgaaaaa
     9481 aatcaataca aaaaagccga ttaagaaata cagcattttc ttttctgata aaaaagcgta
     9541 caaggaaaaa tccttggctc tttctggtct cgttaatggg aaaaagaccc atataaaagg
     9601 aagaacaacg atcatccatc ctaaaagagc cactaacgtt tgccaaatct ttaaccagta
     9661 attacctttt gaaaaatact tatctttcac caaaattgat gcaacttctt ctcttgttac
     9721 ttttttcata tcctcacctc acgttttttt gttacgacgt aggagtttac catacatttc
     9781 atttcttttc acataaagtg ttcctaaatt tttcctaaga aaaagaacaa aacatttctc
     9841 atcagcaaat gaaaaatatt ttgttctttt tatcagttgt tctttttatt ttttatctct
     9901 taattatgtt gctctttcca atacgacaat gcttttcatt cctctagata tcgccgtgta
     9961 catttgtctg cgtcttcttt gctcatctcc atcgttttca aatggatata agattacatg
    10021 atcaaattcc agtccttttg ctagactcaa aggaatgatt tggcagtttt ctaatccata
    10081 tgaatttttt ttaatcgatt cttgtagcgt ttgggtttcc tcttctgttt gggtgataac
    10141 tgccgctttt tctcctttta gagaaaccaa aatctcagct agtattttca acaattcttc
    10201 agaggatcga aaagagcggt attcgggttc ttttccttgc ggtcggatcg atacgatgtc
    10261 gatcttctct tgatccacag cataggtttt gaaaagctct gtgattgctc cgctagaacg
    10321 gtaactattt ctaagatcat accgatggat cggcagatgg tagtcgtcga aacaacgcat
    10381 aatatcagca aaagtagtgg aagaattaaa gattgcctga ttctcgtcac ctactaaagt
    10441 aaagtgagtt tttggaaata attcgatcaa tagacctaat tgtgccaggg tataatcttg
    10501 tacttcatca atcaatagat agcgaaatgc tggtacattt accttttcaa caagtaaatg
    10561 acgaatagct aatataatga ctgcttcatc aacagtacgt gtgggttgcc aagcataagc
    10621 tgagccatat cgtttttcat aaattctttc aaacaactga tgctcatcca cccatgccat
    10681 ttcttcgatt tgtctggtta tttttcgata ttttttttgt aaaagtttcc ttgcataagc
    10741 tacaatactt tgttccgaat catcagaaat caatttaccg aataattcca actgcctatc
    10801 ttctgtcaac gagagtacct gattctgcac agcaggactt tttgcttgtt tcaaaagatg
    10861 attcttccat tcgctcatca gtgccttttt cgttgcttga agcctatcga tcaatttagg
    10921 atgagcagga actttctcat atattttttc gatcgttttt ttagaaagga ctgtttttcc
    10981 atttttagta agtccacgga taaaattcgg atgatctaaa aacaaaggat ctgattgttt
    11041 tagattatcg ataaactttt tgctacgcaa acgttctgtc tgctccgata cattttctcc
    11101 cgtgatacgt ttgaaataag cctcttcacc ttctatttcc tctgctgaaa gctgtgaaac
    11161 cagctgtatg atggttagat tcagtggatt tctttcacct aatgatggta ggacattcga
    11221 aatatagtcg ataaaccgat gattaggtga tagaatcagc aagtcatcgg atgtcatcgt
    11281 atctcgatat aaatagagca gatacgcaat acgctgcatg atagcagagg ttttaccact
    11341 tcctgctacg ccatttacca agatattttg acttttcaca tcccgaatga ttgtgttttg
    11401 ttccgattgg atcgtagcag tgatagcctt catttcattg gtctcatctt ggcctaagac
    11461 ttctaataat acgtcatctt gaatagctac agacgtatca aaaaacttga ccaaacgatc
    11521 tctttccaaa agaaattgtc gtctttcttt aataaccgta tcgattttat tttgacgaac
    11581 cgtataagaa gatggaccaa tcatgttatt gtaaaaaagt tcggcaattg gtgaacgcca
    11641 atcataaaca agattttcat ctgcatcatc tgtgaaattt gctgtaccga tataaaagga
    11701 ttcttttggc tcattatcca gaaagtctac aacgactttt ccaaagtagg gacttttcaa
    11761 taaacgttcg atctttgcta gctgattttc tgcgtttttc tgtttgatat tcatctgatc
    11821 gatttctcta tttttcattt caatcatcga aaaagtatcc aggttatcta agtaactgtc
    11881 aaaattcaat cgaagctcgc cagccatttc tttcaacgac tggtttcctt ctatttttgc
    11941 cgtatccatc gattcccgca gttcgttttt tttatcaatt agcttttgat acacgttctt
    12001 cagatatgct tcttctaact gtttttgtgt gttgaccata cacattctcc tcttttttta
    12061 aagtggtgga accgattagt ttacctcttt taagagtcga atgcaagctt tttaacgagt
    12121 atcatttgtt aggatatatc agttcttcag gtcggacagt ttgatataaa gaaaatgcac
    12181 catcaaggtt ttttacgtgg tagccagact gcttcaagat tctttccgct agatagctac
    12241 gtaatccgct atgacagctg acgatatatt cttgttggct gtctaattca tttaatcggt
    12301 cccgaagttc attcaaaggg atatgtttgg cttccttgaa tttgcccttt tccaattctt
    12361 ctgctgttcg cacatctaat aaaatttttc cttttgctag ttctgtcggc aattcatgcc
    12421 attgtacggt ttcactaagc ccttctgcaa tgttcattgc tgcatatcct agcatgttta
    12481 ctggatcttt ggctgagcca aatggcggtg catacgtcaa ttctaattcc ggtaaatcga
    12541 agatcgtcaa atgccctttg attgctgttg ctaaaatatc gatccgttta tccacacctt
    12601 ttgccccaac accttgtgca ccataaatct cgccagtctc tggatggaag atcaacttca
    12661 gtaaaatatc tgttgcttct ggataatagc ttgcatgatc ttttcctgat acatgaatga
    12721 cttcaaaaga cagttcgttt tctttggctg ttctttcact taaaccggtg gatgcggcag
    12781 tcattccaaa cgttcgtata attgctgttc caataccgcc ttgatttctt cgagaaattc
    12841 cggaaatcgt gtccgcaact tgtctacctt gacgattggc tggtgaagca agagaaataa
    12901 gtgcatcttt tccagtgatt tgctgtttta caacgatagc atcccccacc gcataaatat
    12961 caggaatatt cgtttgataa cgttcgtcta ctaggatacc cccgcgtatg cctaattcga
    13021 ttccagcggt cactgctagc tggttatcag gacgaacacc aatggataaa ataactaaat
    13081 cagcagggat ggtacgccca tcatccagtc gcaatcgttt cccgtggtct tcaaatccag
    13141 cgacagcatg agaagtaatc acttctactc cttgatgaga caattctttt tctataaaag
    13201 ctgccatttc ttcatccagt gttggaagaa tatgtggtgc ttgttcgatc accatgactt
    13261 ctaaacctct tctttttagg ttttctgcca tttccaatcc gatgaatcct gcaccaacga
    13321 tcacggcacg ctttggctgt ttttctaatg catgcatcac tttatcgata tctggtacat
    13381 ttctaataga aaaaacattg tcggcttctg ttatccctgg aatcgatgga acaactggtt
    13441 ttgcaccagg agataaaacc aatgcatcgt actgttcaat gtgtttttga ccctcatgta
    13501 cgacttctac cgttttgttt tcgggaaaga tggctgtcac ttcatgattt ggacgcacat
    13561 ctaaacaaaa acgagcggat aatgattctg gcgtttggac aagaagattt tcccgttcac
    13621 tgatttctcc cgaaagataa taaggcaagc cgcaatttgc aaaagaaaca tacggtcctt
    13681 tttcaaaaac aacgatttct gcatcttcca ttaatcggcg aagccgtgta gcagcagaca
    13741 ttccacctgc tacacctccg acaataacga ttttcatctt atttccctcc tactgttcga
    13801 tcaaaccatt gattcatgcc accacgtaca ttcactacat cataaccaga tttctttaat
    13861 tctttagctg cttgtttact cctcatccct gattgacaaa tcacatagac ggtcttttct
    13921 ttgtctccat catatctgtt tatactttgt aatggaacat ttttagctcc ttttatatgc
    13981 ccgccccggt attctgcagg tgttctaaca tcaagtaacg ctggattttc caaaagcttt
    14041 tcctttaatt ctgggactgt tatcgatttt attgattgaa atagcaaatt cattttctgc
    14101 acctcattta catttttata aatacctcat ggggtatatt aaacaggagc tttattaata
    14161 tgtcaaccag aatgactctt catttttttc actacttttt atatttgcat aaaataccga
    14221 taaggggtat tatctaatta taaaaataga ggagaatgtg ttatggagat tgataataaa
    14281 aatatcctga accgtttgaa acgtacagag ggacaaattc gtggtatcca aaaaatgatc
    14341 gaagacgaaa aagaatgtat ggatgtcatc actcaattaa gcgctgttcg ttctagtatt
    14401 gatcgcgtaa tgggcatgat cgttgcagat aatctgaaaa attgctttga gaaacctgat
    14461 agtaatccag aagaacaagc ggctaaacta gaacaagcga tcaagatgat tattaaaaag
    14521 taaaggaatg atttcaatga aagtcgtttt ccatattgat gaattagaaa aatggtcaga
    14581 aacagggaaa aacgtaaaaa acctgataaa agcttctcca gaaacgacga ttgtcgtatc
    14641 tgtaaatggc atcgcgatta ccggttatct agactctgcc aatgcagaat tcttagattt
    14701 gcaaggagtc gtttttcacg cttgcgccaa tgctatgcgt gctaatcata tttcagaaag
    14761 ttctttacct gaacaagtaa tcgttgtacc agcaggtgtc cttgatttag ttgaactgca
    14821 atcccaaggt tatgcttata tcaagcctta gtcagtagag gttgcgacag aagtgatcag
    14881 cttcaagaaa tttcggctta tttttcgaag aagctacttc tggaaaaccg tgaatcaaag
    14941 gttgtgaaaa cgctgtcctg catcaagtaa taagaacagc caaacaaaaa tagcttctca
    15001 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga
    15061 agaggttgtg aaatcagcgg tttgacctaa gtattagaga aaaaaacggg aaaatagctc
    15121 ctcatatttt gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttaag
    15181 gaagagattg tgaaaaagtc gcttagtttc gagtattaag aaaaattttg tgaaaatgga
    15241 tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg aataccgttt
    15301 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat
    15361 agctgctcat attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt
    15421 ttaccaagag gttgtgagcc taccatttaa ctccgaatca caagaaataa tttcaaaaac
    15481 aacttctcct gtttttggtt cggttggctt attatcgaga agtagctact gggagtctat
    15541 ttatcaaaaa aaggaatgcg acataggtca cacccctttt ttattttctt tgaaacatct
    15601 ttcggtactc gacagcatac aagacaaaag attgtaaaag accgtaagtg atgacggcaa
    15661 aaatgactct cacccacagc gttggtaatt ctgggaaaag cagcaaaagg atcatgccaa
    15721 catagatgac agcagtcgct actttgccat accacttcgc tccacttaat ttgatatttt
    15781 tagatagccc gattaatcca gctacaaaca taaagccttc ttttatcaag aatagaccaa
    15841 acaacagcca aagccacggt cgataccatg ccatagacaa aatcagaaaa agttgtgtca
    15901 atttatctgc aaacggatca agcaccttcc ctaagtctgt tacctgatta tatctccgag
    15961 caatgacccc gtctagataa tcagacaaac ctgagacaac aatgaccgct cctgctatat
    16021 aaaatgactg tctcagcaca aaataaagat aaatcggcag caaaaaaata cggaatatcg
    16081 ataaaagatt cggaatcgtt tgtatttctt tacgccagtc tgtgcttaat ttcattttat
    16141 cgccctacct cctcagcact cttttctttt tattttagct tacacgggga ataaaaccaa
    16201 tgatttcact gtttttattt ttctttacag acactgatat tatgagctaa tttgagataa
    16261 tcacttgctg ttttcatcgt ttactcttct ttcttcctgt cgtttgcgca tttcttttac
    16321 tagtacttta tgagcttttt ttgatcctcg ctctaggtca cgctgccgtt tacgtgtttt
    16381 ataatcgata aacagactgt ctaaatcgta acctgtcggg tacagatctt ctgctgaacg
    16441 caccagacga acacgctgac gaggcacgtg gattattttt ttgtctaaat atacatcaat
    16501 cgtatcttca cctatatctt cataaactaa cgcttctttt tggtattcat tgacaaatat
    16561 ccgatcacct tttgcaaaga gctgacgttc ttttttattc tccttcctat ttgtaactgc
    16621 tgtcttagga agaaattctt ttttctgtgt ccgataggct ttttccgcca agtaacgctc
    16681 tgcttgatgg atcaatttca tcgacatatc cattttatga gcaatccaca aagcttggct
    16741 ttctcctgtt tcttccaacc cgcaactgat atttaggacg aagtgcttcc cgatcgaaag
    16801 ccatagcagc cggcacaaaa tcttcatgat ctccggcgaa tttcttgatt tctccataat
    16861 gggtcgtggc aatgatcaat gccccttgct catacataga ttccatgatt gcgatcgcta
    16921 gtgcagcccc ttcatttggc tctgttccac tgccaatctc atctaaaagt accaacgtat
    16981 tcctttttat ctttttcaaa atagcagcga tgttttgcat atgaccagaa aatgtactca
    17041 acgcattttc taaattttgg tggtcaccga catcgacgaa aatctcatca aagacagcaa
    17101 gctctgtccc ttcttttgcc ggaacttgca taccgaacat cgccatcaaa gtcaataaac
    17161 cgactgtctt caacacgact gttttgcctc cggcatttgc tccggtgata atcaagcctc
    17221 gatagtcttt accgatttca acatccaatg gtaccgcatg ctcaggtaaa aatggatgtc
    17281 tgccttgctt gataatgata tgttccgatt tattgattct aggtgtgctt ccattgattt
    17341 ctcggctaaa ttttgcccga gcaaagatga tatctaacac cgtgatcgtt tcgatcaata
    17401 aatcgatcac ttcttcgttt tctgcgatag caccagtcaa tgccgctagg acttgatatt
    17461 cttcactgat ttcttcagct tttaacaact gatagtgttc attcaattta gaaatcacag
    17521 ttggttcaat aaaaaccgtc gttcttttat ttgattcatc gataatcgtt ccatctattt
    17581 tatttttata gctggctttg ataggaatcg tatagtattc gcctttttga acaatcattg
    17641 cttcttggat catttctttg tttttcggat gacgcaagaa tttcagtaat tttgactgga
    17701 tttctttttc gattagttgt aattgtttgc gtactttacg caaattacgt gacgcgtcgt
    17761 cactgacttt attatttttt attttctggt caatcaactc ttctacattt atcaaatcag
    17821 gaagatgttt gctataagca aacaacaagg gtgtctggta ttgattctta tcaaaaaact
    17881 ttgtgatcat ccgactgctt ctaagaaaat ccgcatattc gatcaaatcg gttggctgca
    17941 gaatcaaccc ttttttgact tgttctgtca gtgcatcgat cctcggcaac cccataaatg
    18001 gaacatgctg actgctttct aagatcaagc gtgcttctct tgtttcttct tgacgatcca
    18061 agacagtttg taggtttgtc gagaccgtca tatcgctgat tcgttttttg ctatagtttc
    18121 caattgctct tgcttggatt tcctttttga tccgatcaaa ttgtaattga ctaatcattt
    18181 cgttgtccat attctctcct tttcttgaag agatacctgt cccaaaataa ataagggcct
    18241 agagataaat aaaaggatag aacacagctt aaaaaagtca tgtcctatcc ttattagaat
    18301 acaaagatat tgttttcctg acaggtaccg tttcataagc acaccaaata aactttgttt
    18361 attggctatt cgacttactg attagatacc agctacactc acaaaacata ccccattctt
    18421 ctaggtctta tttattttcc acttaactat agcaagacac attttggttg tcaaacttta
    18481 gtatgtttta tagatttaca ccattaatga atagacacct accgcgataa ttgctccgat
    18541 cattggacct gcgattggta cccatgaata tgcccaatct gaagttcctt tattagctat
    18601 tggcaatacc gcatgtgcta atcgaggacc aaagtcccga gcagggttga ttgcatatcc
    18661 tgttggccct cctaatgaaa gaccgatggc taagatcaat ccacctacag cgaatacgtt
    18721 cgttccaggt gcaaattcgt tttgtccaaa agctagcaac ccaagaacta atacaaatgt
    18781 accaattaat tctgtgatca catttgctgg ataattccgt atagcaggac ctgttgcaaa
    18841 cgttcctaaa atcgcgcttt catctttcgt tgcattccaa tgtggcaaat aagaaagcca
    18901 aacaagaatg gcccctgcaa aagctcctaa aacttgcgca atgatgaaag gaacgacaag
    18961 attccaacta attgcccctg tcatcgccat agctagagag actgcaggat tcaaatgagc
    19021 tgggcccatg aatccagaaa tatagacagc cattgttaca gcgagtcccc aaccaaatgc
    19081 gatgacaatc cagccagaag cctgtgcttt acttttgttt aaattcacag ccgcacacac
    19141 accatctcca agcaaaacta ggatcagtgt cccaataaac tcccccatga tttgagtcat
    19201 atcactgttc atttatttct ccccctttag ttcccgcaga tctgattctg caatcaatgc
    19261 ttctaaacgt ttagtctgtt gcgccttctc ctcttctgtc cattcaaaat atccagcaat
    19321 tgcatcgatc acaggttgct tgatctcatc aagctgatct cgttcaaaca acagatgatt
    19381 tgtacgacga atgagataat cgcctggggc caaaaccatt tcttcttcta aaccataacg
    19441 taaccgagct gactcagcga gactcaaacc aggataaggt gccatttctt ttgctaattc
    19501 aaagatacgt cgggcattag tcccgtaaaa atcagcgata taagtagcat cttcttcctc
    19561 taaaccggct gctactcctt cttttgctaa ttctgtcact gtttcttcta atttagttgg
    19621 atcgaagttt ccacctgaaa tctgatattt tttagagtcg atttctttcg tctctattcc
    19681 gtattcttct tttaataatt ggcgtatcaa tcgcaaagct ccttctgcca tcttacggta
    19741 atcggtgatt ttcccaccag ataaagtcag caaaccatct ggttctcttt ctaaagaact
    19801 acctctggaa atcgtagaag gtgctttttc atcacggctg ttttccaaat ggttcaacac
    19861 gtcttctact tcagcacgag aaactttgtt ttccttatat tcacttactg tatcgacaac
    19921 tttattgaag ctcttgtctg aaatcgatcc attatctcca ccattgtaat cagaaccaga
    19981 attaccaatc aacagtggac gaagtcctgc ccagcttgct tcgatatcat tcaatgtgat
    20041 atttgcttct ggatagcgat gattgatcac atccaataga taatctacat cttcttgtgt
    20101 gactttcgga tccgtaaaat ctccttggta atccgtatct gtcgtaccaa agtaagtctt
    20161 gttttctctt ggaatagcaa aaaccatccg cccatcttgc tttcctgtat cgaagtatgt
    20221 gggttgcggt acaggcagtt ttttcgcatc tacaactaaa tggatccctt tagttggacg
    20281 cattttagga gagactggac gcgtaaaatt caagtttcgt actttatcta cccaaggacc
    20341 actcgtattg atcactaatt ttgctttgat ttcgatcact tcatctgtca gcaggtcacg
    20401 agctttgacg ccaacgattt gatcgccatc atataaaaag ccaaccgctt tcattttact
    20461 tactagataa gccccatctt cggcagcctt tttgatatta tcaatcacta aacgggcatc
    20521 attgttgcgg aaatctagat agacacccgc gccttttagc ccttcttttt tcaaaaacgg
    20581 ttctctttcc aatacttctt ctggtgtcag cgtataattc tcatatttgg ttcctgtcac
    20641 atttgccagt ttgtcataaa ggtccattgc tactttgacc gagaacatat tgaaggttgt
    20701 tgctccttcg ccctcataga ttggcaaaag cattggatct ggttttggga tgtgtggggc
    20761 aatactttgt acgactgcac gttcaccaac tgtgtcagct actacttcca catcaaatgt
    20821 cttcagataa cgaataccgc catgaacaag tttggtcgag cgagaggaag tcccttctgc
    20881 aaaatcctgc atttcgatca atcctgtttt gattcctgat gctgctgact ggactgccac
    20941 accggcacca gtgattccgc caccgatgat caacagatcc aattcttctt gctgcatttt
    21001 ttgaatgcta tcttgtcttg tcttgtttga aaacatatgg atttcctcct tattcaaatg
    21061 cttgggtagc ttttactgct tttttccaac cgttgtaaag tttttctcgg cgctcttcct
    21121 ccatttgaac ttcaaatagc ttgccagctt cataaaattc gcggatctcg tctgtatctt
    21181 tccaataacc gacagctaat cctgctaaaa atgctgcccc tagcgccgtt gtttccaagt
    21241 tttctgctcg ttggatcggt acattcaaga tatctgtttg gaattgcatc agatattcat
    21301 tgtttgctgc accgccatct actttcaata caggaatctt gattccagtg tcatcttgca
    21361 tcgtatcgat gatatctctc acttgataag caatcgactg taaagtagct ttaatgatat
    21421 cttcttttgt cgtaccacgt gtcaagccaa acatagcccc tcgtgctgcc tgatcccaat
    21481 aaggagcacc tagcccaacg aatgcaggaa caacataaac ctcatcttcg cttgtagagc
    21541 gtttcgctgc gtcttctgaa tcgctagctt tttgcaacat ttgaagtcca tctctcagcc
    21601 attgaatcga agaaccagcc acaaaaatac ttccttctaa agcatagtaa acctttccat
    21661 ttatgccgta accaattgtc gtcaataaat tatttttgga taattgcggt ttttcaccgg
    21721 tattcatgac aataaatgaa cctgtaccat aagtattctt gaccatgcct ggttcgaatg
    21781 ccatttgtcc gaataatgcg gcttgctggt ctcctgccat ccctgaaatt ggtgcttcgc
    21841 ttccataaaa atgataacct tgcgtataac cataaacttc tgagttagaa gtgacttttg
    21901 gtaacatcac tcgaggaata tttagtaaat ctaaaatctc ctgatcccaa tctagctgat
    21961 ggatattgaa caacatcgtc cgactggcat tcgagtaatc agtaacatgt gcttgtccat
    22021 cggtcagttt ccatactaac caagaatcga tcgttccaaa catcaattcg cctttttcag
    22081 ctcgttcttg cgcgccttct acatgatcaa gaatccagcg tatcttcgtt gctgagaaat
    22141 aggcatcgat cactaaccct gttttttcgt ggaaaaattc ttgatagcct tcatttttta
    22201 agttatctgc aattccagct gactgtctgg attgccaaac aactgcatga tagattggac
    22261 gtcctgtatc tttttcccat attactgttg tttcacgctg gttcgttatg ccaatcccag
    22321 caatctgatt tggtttgatc cctgattcga tgaacgctcc ggcaataact gactgtacag
    22381 aattccagat ttcatttgca tcatgttcca cccaactttc ttgcgggaaa tattgcgtga
    22441 actctttttg agaactgccg atgtgtcttc ctttcttgtc aaagataatt gctcgtgagc
    22501 tagttgtacc ttggtcaatc gccatgatat acgttgattc tgtcattgtg attccctcca
    22561 ttatatgcaa acgctttctt taactactct tatcatacta ctgttttaaa aaaagaaact
    22621 gtcaaaattt tattcttttt ttcttttttt gacagttttt tattatgaag ctatttttta
    22681 atcaattctt ttcttttttc agcgtctcta ttcttctttt caatcgaata atgtcgtaag
    22741 gtgacgcaaa ttctgacaga taatattcac actgtttttt gtttctctgg caaaatgtga
    22801 tcaccaaatc atattcttta ccttcttcat aacttccaat ctcaataccg ccgattcccc
    22861 tcagttctct gatcagatat tgttgaaaag caatacgata aatcggcaga ctttctaaat
    22921 cgattccaat cactactggt tttgcaatat aaaaatcgat catcaaaaga acattggcat
    22981 actggatcat caggtaacta agctcactat cttcagctct tttcctcggc aattgctgca
    23041 ccgctgtttc ctgtaatttc gccagtaact gtgttaagga atgcccgagc attttctgct
    23101 gctgttccaa aagccgatca cgctcataga cttcgatctt tccaacaaag aaaaaccatt
    23161 cattgttgat ctgggaaatt tggtagccta ctgccttttc ttctttgatt ttcaaacgac
    23221 gaggacgata atgccataac atcgtttccc gaatgtaagt atctaaaaca gcggttggta
    23281 actttttgct ccgtgtcaaa tcatactgat aaaatatttc ttcgtctact atcgaaaaag
    23341 aaacaaaaaa gctgtaaaac atcattgttt cgtatacatt cagttctgct gctgttcgac
    23401 taagatgcat cacgatgatt ggatcgattg cttgataaag ccggtccgac tgatagagta
    23461 atttcttttc tttcaaagtt gcgtacgtac ttttttcatt caatagtctt tttttcgtaa
    23521 tcccgagcca gcaagcaatc tgtgcttctg ctgatgccgt aaacgttgtt tctaatacac
    23581 ggtccaatcc tagaatgaac ggtctttttt ctggtgtatt ttgcaaaaaa agcggtcgct
    23641 gatcttccgg gatatattgg aatagttcat aataaaaata tcgtatctgt agctcttctc
    23701 cgtataattg accgtttttg atttgtaatc caaattcctc taagagctgg ttcaattctt
    23761 tgatctttcg aaaaacagaa gactcactga tattaaacgc tgtcgctaat cgaacgatgg
    23821 atacttgctg atgttctaat aaatagacga gcatcttaaa cttcaagctt tcctgcaata
    23881 aaaacaatag aattttttcc aaacttacat cttcgtccaa cacaagcagc gcctgatact
    23941 catttcgctg tacttctact tttttttcca tcattcggcc aatcaattca atatcttcca
    24001 aatattgatc aaacgaggct tttgacagtt ggacatgctc tctcaaacca ttgatagagg
    24061 ctgttccccc ttgcagaaca aggtttcgca aaatcaggat ctgtaactgc tcttttcttt
    24121 ctaataatgc ttcaatcttc ataggcagta ttcgccacct ttgaccacac catatctggc
    24181 acacggtatt ctggctggaa aaatagctca tgttcttcta actgttcaaa cgtccatcct
    24241 tcttctactg caattgtcag ttggtcaaga aggtggaaca tctctttttt gctttggagc
    24301 tgtgctccaa gaatcttttt ttcagaatca aatagaaatt tgagtttaac tttgctttga
    24361 tttattgctg taagcttgta gtctacgtat cctgtttcaa ttttttgggg ataaaaaatg
    24421 ctctcttctt ctgtcaaacc tacgcttcct aagaaatagc caaaccactg attgccgact
    24481 ggacgatagg ttccctgatc ctttgtctgt gagccactaa ttgtcttgct gacatttcgt
    24541 gctgtacgga cagcgttatt tactagagaa acatacattt tttcatttgt tggacgaaaa
    24601 gttacttgga tcgcatcgcc aatcgcataa acgtcttttt gagacgtttg caagtagtca
    24661 tctacccaga tcgttccatc atcattcaat gttaattgtt cttcccacat ctgattgtcg
    24721 ggacgagaat gattagccag caaaacaaga tcatagttct cctttctttt ttccgtctct
    24781 acaatagcgg tttcattttc ggtcaaagac acaacttgtt cttctaaaaa aagtgaaagc
    24841 ccttgatttt tcatttcctt tactaacggt tctgtcattt caggatctag ataacgaaaa
    24901 agaattgact ttcggctttc gtaaagatgg atttgttttt ttcttgatgc caacccttca
    24961 gctatttcga ttcctgcttg tcctgcacca ataattgcaa ttttctttgc attcatgatc
    25021 ttttgctgaa gagccgttac gttttgtatc tttttaactg ggtgaataag cgttgattct
    25081 gcagaaatat ttcgaaactt ttgatttgaa ccagtcgcta gaatcaaacg atcaaaatcc
    25141 aatttttttc ctcctgctaa aacgactttt ttttctttca atcctattac tgtcttttct
    25201 gtgtaaacat ggattcgata gcggtcagtc agttcttctt tcgtgatcca agaaagttgt
    25261 ttttcactgg taacctttcc ttttaatatc aaattgaacc cgctgggaat ccatcctaac
    25321 ttcgattgct tttcaatcaa atatatttca cttgttggca tcaattggcg aagataaata
    25381 gccgcatgaa tccctgcaaa cgacccgccg acaattacaa tgttcatctg cattcctctt
    25441 tcttataagg tgttctactg ctaacttctc aatcagaaaa cacagtattc ataactcgct
    25501 ttcaacctta tgatacatag cgatgcagca gaatcaaccc tctccatcat accgtgttct
    25561 aggtctgctt ctcaataatg tactagtgga cctaattgaa gagtatcatt tctgaagaga
    25621 tgtttcaaaa aatgtactct tataaaagaa aaaaactcgc cttttacgta cctctgtcca
    25681 aataggaaac agaaaagaac gaaaagacga gttttagcgt gtgtaatgta atagttagaa
    25741 tagacacatc atttattttt aattctttaa tgcttcaaca actgcttcgc ctaattgatg
    25801 agcagattga ggattttgcc ctgtaagaag ccgcccatca atttgaacca cttctgaaaa
    25861 agctgctcca gactgatagt ttgctccttt ttctttcaaa gcatcttctg ctaagaatgg
    25921 tacttcattc gttgtaccat ttgcttcctc ttcttcatta ctgaacccag tcactgtacg
    25981 tccagctaaa atagattttc cagactcatc tgtaactggc aacaatccta caacaccgtg
    26041 acaaacagct gagaccagcc cttcatttcg atagatttgt gaagcgactt tcccaagatc
    26101 tattgcttct ggaaaatccc acattgttcc gtggccacca gcataataaa ccgctgcata
    26161 atcatttgca tccacatcat caggacgtaa ggtatttccc aaggcccgat ttctgaaatc
    26221 ttcatcttca taataggccc aatcgatttc atccatattc aaaacacttt gcggttctaa
    26281 aggtacatat ccgcctttag gactgacaaa atctgcatcg atcccatttt ttactaatac
    26341 atcataaaaa tgagtcagtt cacttaacca caaacctgta ggacgctcga tcttttcgta
    26401 cttttctaca tttgttaata cgatcaatac tttcattttc ccatcctctt tctataaaaa
    26461 ttgcgttatt gataacgtgc tttcaagaaa ttatataatg ggcttggcaa ataaagctct
    26521 ttatatgctt cttcaaaagg tttcgttaca aattcatcct cacggttttc caataatttc
    26581 tctgcccagt caggatcgac gatcatttgc tgtccaattg ctgcaagttc tgcatatttc
    26641 aatacctgct ctacatcttc gcgattgcgg atcccaccga caccaactaa agggatcttt
    26701 ccttgaatcg tgttatggac cacttcaaga atcgatttat cgttgtattt atcagaacga
    26761 gctattcgat catacatatt taatgaaaca tgcagataat ctagttttga ctcacgtaat
    26821 cgttcaagaa gccagatcgt gtcttcaaaa cggatacctg gtgtttcata ttcttctggt
    26881 gagaaacgat accctacgat gaatggtttt ttagcatatt gttcaaccgc tgagaaaacc
    26941 gttgttaata attcttcaat aaaacgataa cgattttcca agctaccgcc ccattgatcc
    27001 tctcgtctat tcgaatgcgg agagaaaaat tgttggatca aatacgtatt tgctccatgc
    27061 aactcgattc catcaaatcc tgattcaatc gctctacgtg tggctttgcc aaatgcttgg
    27121 attgtttcaa cgatttcttc ttcactcatt gctcgtggtg tttccgcatc aggacgtttt
    27181 gctgcaactg cacttgcaga tacaggctgt tctccagata aagttgctcg acttgtcatt
    27241 cgtccaccgt ggaaaatctg aataatcgct tttgcgcctt ctccttggat tgctttcgct
    27301 agctcactta atcgaggaat catttcatca tgagcgatgc tgagttcacc tggccagcct
    27361 tttccgatat cttggacatt tgcagcacca gtaataacag caccaagacc tttagaacgt
    27421 tgtttataat aagtgatctc atcttcggtg atcactccat tgaaaaagct gagttgtgtc
    27481 gtcataggcg acagcatcaa tcggttcttc aattgaactc ctgaaggtaa agtaattgat
    27541 tgatcaaatt gtgacattgt cattcctccc ataattcgct ctgattaacc tatcgagaat
    27601 catctatcat taaaccactc gattagttgt acatgcaact aatttgctta aaaaatttta
    27661 ttttttttgt aacttatctg ttgcatcatt cattgtctgg gctaattctt tacttgcttc
    27721 atttcccaaa actgctttgt atgactgatg aagttcttcc cagctaccat cgacttcctt
    27781 agcaaattgg cgtccttgtt ctgtaagata aactaatgac aagcgacctt cgctaactgt
    27841 tttgacgtat cctttatatt ctaatttttc aacaaaacga gcaatcgttg atggggcgga
    27901 atagagcatt tgtgcaatat ctttttgtgg tattccatct ttttctttta atatcaataa
    27961 aataaaacca taagttggtg ccagacctgt ccttctaaac gcttcatcag ctaatttatt
    28021 caacatgcga tctagctttt tcacgctaaa gaaaagacat tcatctaacg ctttttcaat
    28081 tggcttttcc atttcatccc ctccattggc gtacatatca tagtatgatt tttatttttt
    28141 gtcaaaaaac acggaataat gtccgcctcc tctgtctaga tttgaaaaaa agaagtagcc
    28201 atcttcaaag aatttcggct tgttttcgac aaaactcctt ctggaacacc gtgaatcaga
    28261 ggttgtgaaa aagtcgttta gttttgagta tgaagaaaaa ttttgtgaaa atggctttcc
    28321 acatttttgc ataaatttgg cttaataccg aaaaactggc ttttgaacac cgtttatatc
    28381 agaggttgtg aaaaagccgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
    28441 ctcatatttt gtcgtttttt gagctaatgc cgatggtcag ctgattgtac accgtttatt
    28501 gagaggtcgt gagcctgccg ttgaactccg agtcacaagg agcaatttca aaaaacagct
    28561 tctcatgttt tatgactatg gtgacttgtg atcgaggagt tggcaggaga atactgttta
    28621 ttcgtaaaga aggggctgtg acaagacttt tgtcacagtc ccttatttcc ttttattata
    28681 ggatttcgcc atttgattgg atgacttgtc tgtaccaatc aaatgatttt ttctttaacc
    28741 gtttctttgt gccattccct tcgtcatctt gatcaacata gatatatcca taacgttttg
    28801 acatttgtga agtagaagcg ctgacgatat caatgcagcc ccacgacgta taaccaaata
    28861 aatctactcc atctttgata gcttccttca tctgctcaat atgctgcttc aaatagtcaa
    28921 tgcggtaatc gtcttgaacg gttccatctt cttctacttt gtctatagct ccgagtccgt
    28981 tttcagctat gaacagtggt ttttgatacc tatcatataa ttgattcaaa gcaattctca
    29041 aacccttagg atcaatctgc cagccccatt cacttgatgg aagatacgga tttttaattc
    29101 ctccagttat taagtttcct tggacccctt cccaattttc tggatgagca ctgactgcac
    29161 tagacatgta gtagctaaaa gaaacaaaat caacagggta ggctgctaag atttcttcat
    29221 cacctacttc tatttttaag ttgacacctt tttcttcaaa cattcgttgc gtatacgctg
    29281 gataatatcc tcttgcttga acatcactaa agaacaaaga agttctttgc tgctcaattg
    29341 ttttttgaat atcatccgga tcacaagaat acggaacaag aagctgatag ctgatcatac
    29401 aaccgacttg tgaacctttg ataatttcat gacaagcttt tgtagctaaa gcacttgcta
    29461 taaactgatg gtgtgcacct tggtatttcg cttgttccaa atggggatgg ttttcttcaa
    29521 ttactcctac gctaacaaaa gggtgatgtt ttacacagtt gatctcattg aaagtcatcc
    29581 aatattttac tttattttta tagcgtttga atactgtttc agcgaaacga acgtaaaact
    29641 gaataagctt tcgattaggc catccaccat attctgtaac caaatataac ggcatttcat
    29701 aatgggaaag agaaacgact ggctcaatcc catattttag acattcgtca aacacttgat
    29761 cataaaaaaa taatcccgct tcattgggtg tttcttcatc accatgagga aaaattcttg
    29821 tccatgcgat agacatccga tagcatttaa aacccatttc agcaaataaa cggatgtctt
    29881 ctttaaagtg atgatagaaa tcatttccgt gtcttttagg ataataatac ttgctgttgg
    29941 gatcctgcgc cgcttttact tgctgatgcg tcatcatatg ccattgatct gtccattttt
    30001 ctttaggtaa atcgctgtcg aatgtataca catcagacac agataatcct ttttcatcta
    30061 catcccatgc accttcacat tgattggccg ctgtcgctcc gccccataaa aatccttctg
    30121 gaaatttttt catatcgatc cccctaaact tgctagtttt cttgtttgat tatttttctt
    30181 atgactagta atgatatttt tgaatctact actatttgta acaataacca ttgtggtcgg
    30241 atcatatccc tctttgatta attcttgcaa atctacttcc atcaataaat cgcctttacg
    30301 tacttttttt ccatcagata ctgcactttg aaagtatttg ccatttaaat tcactgtatc
    30361 aataccgata tggatcagta tctctgctcc ttcttcactg actaccccaa ttgcatgttt
    30421 cgttggaaaa actgttgaaa cttccccgtt tacagaactg tagatttttc cgtccgttgg
    30481 aatgatcgca aacccttctc ctaaagatcc actagcaaat gttggatcgt ttacttcaga
    30541 taaatcaatt atttcacctg ctgcaggagt acttaacact atatcctttg aggattcttc
    30601 ttcgatttca tccttgtagc cgaaaagaaa agttagcata caggaactga tcatcgctaa
    30661 gaaataacta gcaactagcc aaacaaatcc ttcaccaata taaatcggga aagacaaaat
    30721 acttcttgta gcaactgcca aagcaccggc tcctgcggca ccagctactc caccaaatat
    30781 agctccacat atacaagcaa tgtaaaatgg tcgtttatat ttaagagtca caccataaac
    30841 agcaggttca gtgattccaa ataatgctgt gataaatgca gataaagata aactttttac
    30901 tttcttattt ttcgttttca aaaatacccc aagtactgct cctgcttgcg cagctacggc
    30961 agtcatacat gctggaccta gcgtatcacg accgtagaaa gaaagattgt tggtcattac
    31021 tggtacaagt ccccaatgta acccaaaaat cactaacact tgccaaaatc cgccgataac
    31081 agcaccagat aaaacagggc taaagttata aacagctaaa tatccagctg caatcagctg
    31141 acttgcataa tctgcaattg gacctacaac taataaaata attgggacca tcacgatgat
    31201 acaaatgaat ggtgtcaata aatttcgaat actttcgtgc aagcttttgc gaatcttagg
    31261 ctcaatataa gaaagaaccc atacagctaa aattgctgga ataaccgtac ttgtatacct
    31321 tgctagaatc acgggcatcc ctagaaatcg caaggttatg ctatcactat atgcactgac
    31381 aatcgtagga taaacgagag ctgctgcaat aaccatagcg atgaaacgat ctgtattgaa
    31441 cttttttgca gcagtatatg ccaaaaatat aggcaagaaa tagaaaaatg catcagctgc
    31501 tgcattcaaa atgatataag ttcctgattc agtagttaat aaattcaaac tcgtacataa
    31561 ggcgagtact cctttgatca aaccacttcc tgccaagacg cctaaagtcg gggcaaaaac
    31621 agcggcgatc atgtctataa aagagtttaa aatgcctttt ttctcagttc ctctctttcc
    31681 actagatgca tcatcttcta gtttcatttg tttggataat acgtcataca cttcatttac
    31741 tgcatttcct actacgactt gaaacattcc tccagcttcg actacagtga tcacaccttc
    31801 tagttgctct accttctcct tatctgcctt cttattatct tttagggtaa agcgtaacct
    31861 tgttgcacaa tggtaaacat tgctgacatt ttcttcgcct ccgacaaatc ggacaatatc
    31921 tttcgctaat tgtgaatagt ccatttttac ttcccccttg ttaattaatg ccgtagcaat
    31981 ccttcttaca agataagtat agttcttaga aaacggtttc tccattcaat tagtacccct
    32041 tctaatagcg aacgaaactg tacaatagtg tgtatggttt acttagccga gcaaatcact
    32101 cactgcttga aagaaaacaa gtatatcgtt tctcttatag tcaaaggaga caaaaaaaac
    32161 acgcttgatc ttcctttgct gatgtataag tggtatctca agctgatact ctgtgatttt
    32221 tgtttctact tggcttttga taagacagca caacaaagtc tcggcattta acggaagcat
    32281 aactaggtca gaactaggag agattagttg ctgaatggaa tctatctttt tatggatttt
    32341 tatttgattt tcttctttgt agtaatgatc aagcagtttt gttttctttt tttcttcaac
    32401 tatggtcagc ttttcacgca aaatctgata aatctcccaa gattcaaact catctagaac
    32461 actaggtaga taaatcaaat catcccactc aaaaataagc tcttgctcag tagtcaaaat
    32521 tgcatcatat tccgttattt tgtttgattg atttttgaaa agatacactg gttctatccg
    32581 gcattctttg atccacatgt tcatactttg ttgaatctgt ttttttagtg tattcaaaat
    32641 actaattggc tggttactaa caatcagtat ctccccttgt tttcgaaaag gattcaacgc
    32701 ttcggcaaga tacaaaacaa aataagcgct ttcagctaaa ttgggacgta attgaaaatg
    32761 tattgggaag aaacgccgaa ttaaataaat ttctaaagga taacgggaga tgatcttttg
    32821 tacaaaatga ttcatggtat tatgtccggc atccatcctc gttttcattt gtttgatatg
    32881 ggttagcaaa aattctgttt catggaacaa taacgaaaca ggaagatcta aaaaacgaat
    32941 aatcgtttgc tcaaatgctc ttagattctt ctctatttct ttgttttttt catcattaat
    33001 catcaaataa tttaactcta atagtcgatt ttcaattgct tgtttttctt gattggaaaa
    33061 agaatagggt agttctgatg ataacttttt tgtgagggtc tctaccatcg gaataaataa
    33121 tgtatacctg tttgtttttt ctaaaagttc ttctcttcga agaatggtca aaacgagata
    33181 ccgagaaaat cgtatgaagt cctctcctga aacaatataa ttatatgata taagaacctc
    33241 ttttaatgtc ttttggacag tcggcatgat attggaaaaa attgtactga tttgttgagg
    33301 aaaccttgcg tgttggatca ccgtttctgt tccaacaaga gataagaaaa ttctttttga
    33361 attttcttca gcaatgattt ttagtccttt tgtcgacgaa acttccagtt ctaatttagg
    33421 attgcgctcg atccaacgca aaatcgtttt tatttgtaat gatacggctg tttttgaaag
    33481 aaagaaaatg tctgctatct tttgcatact gacataccct ttttgaaaca ataagtacgt
    33541 cacaatagtt gaagctcgga aatccatgct ggaatagatt ttatgtttcg tcacctcttg
    33601 tatgatttgc tgtttagttt cagattcaat tgattctaaa aaataacctg aacgtttatt
    33661 tgactggata atgattttct tctcgtagat agcaagcata tttttgattt cagtttggat
    33721 cgttttcttg ttggtaccta aagcttcagc taattcttcg cttgttatcc aatgattctt
    33781 atttcttaat actacaagaa tttggagttg tcgtttattt aagtccattt gcttacacct
    33841 cctctttgtt ataaacatac aggaaaaacc tctcaaatga caatagtcac ttgcagaggt
    33901 ttaaaaatca tttgaagaaa gatctatatt caaggttttt accattagac tggataattt
    33961 gtttataata ttcaaaacta tcttttttca atctcctaaa gcttccgctc ccatcatttt
    34021 gtctatccac ataaataaag ccatatcgct tcctcatttc ccctgttcct gccgatacga
    34081 tatcgattgg tccccaccat aagtaaccaa tgatttggca accatctttt attgcctcag
    34141 caatttcctg aagatgtatt tttagatact cacaacgttt tggatcgtgg attcgattca
    34201 attcatctgg tgcttcatct agtccaatgc cattttcaac tatgaataat ggtaattcat
    34261 aacggtctgt caattcattc atgacataac gaaaccctag aggatcaatt ggccaactcc
    34321 aaggttcggg agatttttcc ttcaaaaagg gattatcgaa accatttgct ccgccagtat
    34381 ctacgcgcat ttctgcttct tttttataga cagcgctacg ataataacta aatgcaatat
    34441 aatctgccgt gttagcttgc aagcactgtt cgtctccatc taacattact ggtgcacagt
    34501 cttcttcctt ccatgtcctg taaatataag ctggataatg ccctcggcac atgacatcgg
    34561 agaaaaacca tgaattgcgt ttaaattgca gtgaaccaaa tacattctct ggatcacagt
    34621 catatgcata agtcgctaat gcagaaaagc tacacataat acccatctta gcatttggat
    34681 cgattttctt taataattga gtagcacggg cgttcgctag aaacatatga tgacaggctt
    34741 gataaatcac tttttttgtt agtgtagcaa tgggttcaag tttatctcta gcagggaatg
    34801 taaccaatcc agcagctgca taaggcatac gaaaagcatt attgatctca ttaaaggtca
    34861 tccaatattt cactttccct tggtagcgtt caaagacggt ctgcgcataa aattcaaaaa
    34921 agtcgattaa ttttcgattt tgccaccccc catactctcc taccaatgct aagggggttt
    34981 cataatgact aagagtaatt actggctcga tatcatatcg atttaatgta ttgattagtc
    35041 tgtcataaaa tacgagacca gcctcatttg gcttctgttc gtctcctctt ggaaatattc
    35101 gtgcccatga gatgcttgtt cgaaaagctt ttaaacctaa ctccttcagt aaacgaatat
    35161 cctcttcaaa tcgatgataa aaatcaatcg cttcatgaga aagataatgg agtgattcgt
    35221 ctaaagcaat ctcccaaatc tttttttctt cattccactg aatactagga tgctggttca
    35281 tgatcccgat aagcgtatct gtcacattag gtaatttgcc gtcttccaac catgcacctt
    35341 cacattgatt agcagctact gctccgcccc ataaaaaatt ttctggaaat atttcctgat
    35401 ccataactat ctctcctcgc tatcaacaat ttcttacttg aagtatagct cgattcatca
    35461 aaacaagata ttctaactat ttcggcagaa gagaagaaaa aaattgaatg ctactcttca
    35521 tttttaatat ttatatataa caaatacata agacgatccc cgaaagaaaa cactcgttaa
    35581 ccatctaagc aaacgattta ttttacttcc aacacaaatg cttcataagg tcgtaaatca
    35641 actttgccgt ctggtaactg tttcagagga taatttgaca caataaccct taaagcttca
    35701 cgtggaatag tgaacgtctg cttttcactg gacatattac aaataacgag ccattgctgg
    35761 tcttcatata tgcgagaata ggcaaagaca tgctcaggcg tcttttccac taattcatag
    35821 tctccccaga cgatgatgtc atgttttcta cgcaaatcga tcaatttctt gtacatataa
    35881 aaaacagaat cttgatctgc taacgcttct tctgcattga tttctttata atttggattg
    35941 actcgaagcc atggaattcc agtagtaaac ccagcatgtt cacgggcgtt ccattgcata
    36001 ggcgtcctag cattgtctct tcctttcgcc ttgattgatt ccaagatttc ctcttttgta
    36061 aatccagaag aaatcctttc gttatacata ttgattgtct caatatcttt tgcttccgcg
    36121 atatcatcga ttgggcaatt cgtcatgcct aactcttctc cttgatagat ataaggggtg
    36181 cctttcatca tatggagcaa gatagcaaac atcttggcac tttcttttcg atacacatcg
    36241 tcatttcccc aacgagaaac aattcgcgga agatcgtgat tattccaaaa taaactattc
    36301 catccttcat cacctaaaga tgtttgccat ttggataata cttttttcag ttcagagata
    36361 ctcaagggtt tcaaatccca tttgtctttg ccttcttgct gatctaatcc tatgtgttcg
    36421 aattggaaga ccatggataa ttcatttctt ttaggattgg aataaagttt agcgatttcg
    36481 gtagttgctc cccatgtttc gccaacagtc atcagatttt tgtcaccaaa agttgcttga
    36541 ttcatttcct gaagatactc atgcagttta ggtccattac ttgtgatttc tttatccggt
    36601 tctttgccaa tcatatcgat cacgtccata cgaaagcccc caataccttt atcgatccag
    36661 aaattcatca tttgataaat ctcttggcga acctgtttat ttttccaatt caaatcaggt
    36721 tgacgcttgc tatacaagtg cagaaaatat tgccccgttt tttcatccaa ctgccaagca
    36781 gaaccactga atattgagcg caatctatta ggctcttttc cattgaccgg atcacgccaa
    36841 atataatagt cccgataagg attttcttta tttttacggg cctcaatgaa ccacggatgt
    36901 tcatcagaag tatgattcac caccagatcc atgatgatac ggatatttct tttctttgct
    36961 tcttcaatca gttcttccat ttcttccatc gtaccgaatt ctgccatgat tgcttgataa
    37021 ttactgatat catacccatt gtcatcattc ggagactcgt atactggaga aagccaaatt
    37081 gcatcaatac ctaaatacgc cagataatcc aacctggaaa taatcccccg caaatctcca
    37141 ataccatctt gatttgaatc ttgaaaactt cttggataga tttgataaac aactgaatgg
    37201 tgccaccatt tttgttccat gattttcctc ctataaatgg atgtttctat tttttgctta
    37261 atcatttatt ttagtctctc tcactaaaaa ttgtaacgct ttcaaatagc tattgcacct
    37321 tgttatcggt aacagaataa aacaaaaaaa cagctgagga aattccctca actgtttttt
    37381 gaaaggattg atttatgcag ggaagaatgc tgatttcaca acttctttat aaacggtgtt
    37441 cactcagctg tcccttggta ttagctcaaa aaacggcaaa atataagaaa ctattttttc
    37501 gtttttttct ctaatactca ggccagaacg ctgatttcac aacctcttta tttcacattc
    37561 aatatccaca tgaatcccac aaatacaaag aacagaccat acatgattgg agatacttct
    37621 tttcctcttt tagctgctaa catggtaatt ggatagaaga taaaacctaa tgcaatccca
    37681 tctgaaatac tataagtcaa aggcatgccg atcaagatca agaatgaagg gatcgcaatc
    37741 tctaagtttc cccattcgat ttgtcgcaaa gattgtgcca tcaatactcc taccataatc
    37801 aatgctggtg cagttacttg tgaagtaaca actgatagta atggagaaaa gaacaagcct
    37861 aacaagaaga aaatccctgt agtaatagct gtgatacctg aacgtcctcc aacggcgata
    37921 ccagctgatg attctacaaa tgcaccaaca ggagaagtac caaataagga acccgcaagc
    37981 atagctgttg aatcagaagc taaagctttc cctacacgag gcatcttgtt gtctttcatg
    38041 aaacctgctt ggtttgctaa tccaactaaa gttcctgctg tatcaaagaa agtcacaagc
    38101 aagaatgtca gcactacgac ccacatttga agagaattga tgtcccctac atgttttaat
    38161 gcaactaaaa atgttggttt caaacttggt gctgcggcaa caatttcaga aggcattgga
    38221 atcaaacctg tgaccagacc taaaatcgtt gtagctgcca tcccgatgaa gatgccacct
    38281 ggtacacgcc gaacaagcaa gatagcagtt acgactaatc caaaaatcgt caaccaagta
    38341 gaaccaacgt tgagtgatcc taatcctact aatgtagatt cattcgaaac gatgatccca
    38401 ccttcgctca gaccaaggaa agcgataaat agaccgatac cgccagaaat cgcatatttc
    38461 aaatctgctg ggatcgcatc aatgatcatt tcccgcagct tgaagattgt gatcaaaata
    38521 aaaatcaaag aagcaacgaa tacgccggct aatgctgttt cccatggaat ccccattcct
    38581 aaacaaacag aataagcaaa aaaagcattg atcccaagag caggtgccgt tccaattgga
    38641 taacgggcta atacccccat taaaacacag ccgatcgcac tggctaatgc agtcgcagta
    38701 aacactgctc cttcatccat tccagaagca cctaatacag ttggatttac aaataagata
    38761 taagccattg agataaatgt tgtaaatcct gccagtacct cacgttttac cgtggtgttc
    38821 aatttttcta tttcgaagta ggaaagtatt ttttccttca tcttccatcc ccctaaatcc
    38881 gaacattata aataaagtcc gttaaaattt cgatttatta ataaaacaac gtgttttatc
    38941 atatactaaa acaaaacata agtaaacaaa aaattcggat tttaagcaaa atccgaacgt
    39001 tccatctgat taaatccctt tatctcaaag ttcatgaaca ttcactttgg attacaagaa
    39061 tttctttatt gtgttaataa agtttcttca ttctagtctc aaaaatcaat agtgtgattt
    39121 gattttacca tttcgtttct gttcttcttt gatgaattct tttgtatcga caggattcag
    39181 attatttagt ccgccaaaac ctgtatcgac catttttccc cagccttttg tcttcttttt
    39241 tcctgatttg agattctttg ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa
    39301 ataaccttct aatcttccca ttagatccac tgtttttcct cctttttatt caatcagacg
    39361 actcagcttt tcctataaca aaataattgc cttcagggtc agaaaaattg aaagtcaaga
    39421 tgccattgtt atctgtgatt tctcctggtt cttccatgtt ctcgtagata gtttcaaatt
    39481 catccgtaaa aaatactaaa gaaggcggtg gtcctaatac ctctggtgaa aacttctgca
    39541 caaatacttt tgggaatacg cctaattcta attcttttga gatgctgagt acaatactct
    39601 taaattcttc tggcaattcg attgtttcag aaatttctgc accaattttt tctacaaaga
    39661 atcggcaaat catctctacg tcattgacat aaagcatcac acgtgttttt tgtatcatca
    39721 tttgaccctt ctttctattt gattttcttt cctgtctata aactgattcc attatatcac
    39781 gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa ataaagcaat agtttctagg
    39841 attgctttcc tccacaaact ttttttacaa aaaaaaaacg gcaatgaatg ccgctcttct
    39901 t
//
LOCUS       DAXWLF010000005        39891 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXWLF010000005 DAXWLF010000000
VERSION     DAXWLF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.005"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACGE4U_000169"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACGE4U_000169"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFF8392365.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACGE4U_000170"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACGE4U_000170"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFF8392366.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACGE4U_000171"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACGE4U_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFF8392367.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACGE4U_000172"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACGE4U_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFF8392368.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACGE4U_000173"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACGE4U_000173"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFF8392369.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACGE4U_000174"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACGE4U_000174"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFF8392370.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ACGE4U_000175"
     CDS             complement(8054..8524)
                     /locus_tag="ACGE4U_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFF8392371.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ACGE4U_000176"
     CDS             complement(8570..8995)
                     /locus_tag="ACGE4U_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFF8392372.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ACGE4U_000177"
     CDS             complement(9062..9331)
                     /locus_tag="ACGE4U_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFF8392373.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ACGE4U_000178"
     CDS             complement(9342..10937)
                     /locus_tag="ACGE4U_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8392374.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACGE4U_000179"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACGE4U_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFF8392375.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACGE4U_000180"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACGE4U_000180"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFF8392376.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="ACGE4U_000181"
     CDS             15403..16377
                     /locus_tag="ACGE4U_000181"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFF8392377.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="ACGE4U_000182"
     CDS             16547..17947
                     /locus_tag="ACGE4U_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFF8392378.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="ACGE4U_000183"
     CDS             complement(18025..18747)
                     /locus_tag="ACGE4U_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8392379.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="ACGE4U_000184"
     CDS             complement(18826..19332)
                     /locus_tag="ACGE4U_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFF8392380.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="ACGE4U_000185"
     CDS             19455..21254
                     /locus_tag="ACGE4U_000185"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFF8392381.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="ACGE4U_000186"
     CDS             complement(21322..22896)
                     /locus_tag="ACGE4U_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFF8392382.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLHQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="ACGE4U_000187"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="ACGE4U_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFF8392383.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="ACGE4U_000188"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="ACGE4U_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFF8392384.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="ACGE4U_000189"
     CDS             complement(24673..25311)
                     /locus_tag="ACGE4U_000189"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFF8392385.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /gene="rpe"
                     /locus_tag="ACGE4U_000190"
     CDS             complement(25312..25962)
                     /gene="rpe"
                     /locus_tag="ACGE4U_000190"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFF8392386.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="ACGE4U_000191"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="ACGE4U_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFF8392387.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="ACGE4U_000192"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="ACGE4U_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFF8392388.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="ACGE4U_000193"
     CDS             complement(29105..29845)
                     /locus_tag="ACGE4U_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFF8392389.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="ACGE4U_000194"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="ACGE4U_000194"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFF8392390.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /gene="fmt"
                     /locus_tag="ACGE4U_000195"
     CDS             complement(31216..32154)
                     /gene="fmt"
                     /locus_tag="ACGE4U_000195"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFF8392391.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="ACGE4U_000196"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="ACGE4U_000196"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFF8392392.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="ACGE4U_000197"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="ACGE4U_000197"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFF8392393.1"
                     /translation="MILTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /gene="rpoZ"
                     /locus_tag="ACGE4U_000198"
     CDS             complement(35314..35619)
                     /gene="rpoZ"
                     /locus_tag="ACGE4U_000198"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFF8392394.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="ACGE4U_000199"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="ACGE4U_000199"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFF8392395.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36725..37009)
                     /locus_tag="ACGE4U_000200"
     CDS             complement(36725..37009)
                     /locus_tag="ACGE4U_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392396.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37027..37917)
                     /locus_tag="ACGE4U_000201"
     CDS             complement(37027..37917)
                     /locus_tag="ACGE4U_000201"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFF8392397.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37937..38155)
                     /locus_tag="ACGE4U_000202"
     CDS             complement(37937..38155)
                     /locus_tag="ACGE4U_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFF8392398.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38125..39825)
                     /locus_tag="ACGE4U_000203"
     CDS             complement(38125..39825)
                     /locus_tag="ACGE4U_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFF8392399.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
BASE COUNT        10773 a         8695 c         7254 g        13169 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgatgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggtaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cctagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttagtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ccaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatatca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgacatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac cgagaaaatg gtttttgaac atcacttatt
    36601 caaaataaaa aaagattgtg acacgaattt tgtcacaacc tttaaatgta ttcctagagc
    36661 aaagaagcta agcgaagaaa tccaattctg attatttatt gtatttcttt gttcagcatc
    36721 cttgctaaaa gcccatattg gacaaagtat tcactacacc catgatctca tcttttcgag
    36781 aacgtgcttt ttctgtcgga tcttctacct cttctgtgtc aattcgaaca ataaatttcc
    36841 gattcatcgt cacgtaagca ccgtcgttga tcaaaataaa cagttcgttc tcattatagt
    36901 ctgcacaggc atctttccat acgtcaaagc cgtttttttc agaagggata tcccgtttaa
    36961 ccacagtttt tccacttaat tcgtggaacg ttacgtcata atatttcatt atctctacct
    37021 ctttcgtcat tcaatatttt gtacttgttc tctgattttt tctaagatcg tcttagactg
    37081 gacgacttga tttttaattt cgattgcatt tgatttggaa ccgatcgtgt tgatttcccg
    37141 attcatttct tggatcagga aatctaattc ccgacctaca ggacattgtg cttcgataag
    37201 ttcatctagt ttttgtacat ggatcgccag acgatccagt tcctcgtgaa tatctccgcg
    37261 ttccaacaag atggctagct ccgtcaacaa ccgttgctca tcgactttcg ctccaagaaa
    37321 ttcctccagc tttttttgat accttatttg aaattccttt tcatagattt ctacaaaact
    37381 atcgagtttt tttagtactt ctttgaattc ttcagtattc tttttcaaca ctaaagctaa
    37441 agcttgacct tctttttctc gactcgcctc aatagaatca agtgcgcttt tcaatgtatc
    37501 tagagctaac tgtgccaatt cttccatgct ctcttccggt ttttcttcta ccgttacgaa
    37561 gttttcgtcc agcgcgaagc gttccgccat ccgactaatt gacacttgat tttgactacc
    37621 gtatcgttgt tgaattccgt ctgtcaattg agtgataagt gaatcaatca attcccagtt
    37681 gatgacgatc tgtttttcgt ttttgccaat ataagctaga ttcacaaata cttcgattcg
    37741 accgcggctt aactgctgtt taataagctg acgaaaatcg ttttcaacaa agttcaggat
    37801 tttaggactt cgtatttgaa catctagaaa acgctggttg acacttttta tttcaatatc
    37861 taactgatac gcttttgttt cgcggatggc tttgccaaaa ccagtcatac ttttcattcg
    37921 acagggtcct tcctcattac atattttcta ttgctttttg cagttgttca aactcttcgt
    37981 tcgtcaatgt caggcctttc cccatttttt catggtctgg cgcccaatct cgaagatcga
    38041 attttggcgg gcgaccattc cagctgacta ggttcagttc tttacgccac cccttgttgt
    38101 tttctgacaa tatagcgatt tcttctacga tttcatatga aaattcttgt gccatggttg
    38161 ttcctccttg agataatata aatataggat cagtaatgtg tcaactggtt gcttaagcaa
    38221 ttcatgctgc aaacgatcct tgcgttttcc ttgtagcaga taatcaaaat aaacagtttt
    38281 cgtgcgctca ttttgtattt tttgctggta ttttcgatac cttcgccatt cttcagtact
    38341 taattgacca atttgtgcca ataaatcatc cggcatactt tttatatctt gttggcagat
    38401 catttgaatc atcatgtcta cttgatagat ttcattgggc agtaaaaaat gtttttttgc
    38461 taaaagttct agcatcgcca atccgcatgt ctctggtacg accctgttct tatataaatt
    38521 acgcagtttt tgccaaaatt ttatgctttt gaaaaaagat aattctaact gaatttcttt
    38581 tgttaagtaa gacaaaaatc ctcgccagta tgttcttgca ggtttaacag aatgaaatgt
    38641 ataccagttc ccatagtaat agcctaatcg ttttttcatt tccagatagc ctaattgtaa
    38701 atttaattgg ttcctttggc tatcaaaaag cagaaagctg cctagtgtcc acaatgtttg
    38761 acatttccaa tggataaaaa tgtctggcat acgaattttt ttcgctgggc ctggcccttg
    38821 cacatctacg acaaatgcct ctgttgctcc tttgttaatc gcaatatcaa taggtatttt
    38881 gttgcgatac ccaccgtctg catatttatt ttttccgatc tttcgataag ccatagcggg
    38941 ataaaatgaa gcagaggcga gtatccaatc tggtatttct tctgtttttg tctgttggat
    39001 atcagtcact acttccgcta gttctggcag tttagtagac accgtatata aacgtatttt
    39061 gggattatgt ttgagtaagg acaaatccaa attgtctttg accatctttt ccaatggagc
    39121 agaagaaacc ccgccttcaa ttagtgctgt tttcgtcatt tggcgtcttt ctcgatagaa
    39181 gcgttcacga aggtcttcaa ctgcagccat ttctggtaac gcaagcactt gtcttgtcga
    39241 cagctttttc cataaattca cagctttttt catatctcct tgtaaaatca atgcgccgtt
    39301 caacgcacct accgaggtac ctgtgataat ctcaaagttg atatcatgtt ccttcagcgc
    39361 ctgccacaca ccaatctgat aagcaccgtg tgctcctcct ccacctaaaa caagtgctgt
    39421 atgatagacc agctttttat ttaattgtgt ggttccttga tagccgtttt tatgaaaaat
    39481 acgcaaccat tcttctgata gaggagattg aaataaaaaa caacagtttt ctttaataaa
    39541 aaaacgagcg caagcttcca tataccgaaa aaattttttc cattcaaaag tttcaccttc
    39601 aatcaacaga ttattaaaaa taacatctcc tctactttta gcaatagtaa aatagacggt
    39661 atattttttc gatactgctt taaaagcaca ttgctttttt gcagaacgcc aaaaagcgtc
    39721 tctactttct tgatgacttt gaaaaaatgg aaggggttgt tttgcttgct ggataggcga
    39781 atacagcgac attttctgtg aaaagtaaac tttttttatc tccatcgcgc tctccttttt
    39841 cttcaagtat accagtacgt cataaaaagg tcatgagtct cgtattcgac t
//
LOCUS       DAXWLF010000006        37826 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXWLF010000006 DAXWLF010000000
VERSION     DAXWLF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.006"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            191..421
                     /locus_tag="ACGE4U_000204"
     CDS             191..421
                     /locus_tag="ACGE4U_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFF8392400.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            518..1819
                     /gene="murA"
                     /locus_tag="ACGE4U_000205"
     CDS             518..1819
                     /gene="murA"
                     /locus_tag="ACGE4U_000205"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFF8392401.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1832..2008
                     /locus_tag="ACGE4U_000206"
     CDS             1832..2008
                     /locus_tag="ACGE4U_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFF8392402.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2143..2439
                     /gene="yidD"
                     /locus_tag="ACGE4U_000207"
     CDS             2143..2439
                     /gene="yidD"
                     /locus_tag="ACGE4U_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFF8392403.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2624..3091
                     /locus_tag="ACGE4U_000208"
     CDS             2624..3091
                     /locus_tag="ACGE4U_000208"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8392404.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3176..4060
                     /locus_tag="ACGE4U_000209"
     CDS             3176..4060
                     /locus_tag="ACGE4U_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8392405.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4174..4410
                     /locus_tag="ACGE4U_000210"
     CDS             4174..4410
                     /locus_tag="ACGE4U_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFF8392406.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4498..4578
                     /locus_tag="ACGE4U_000211"
     tRNA            4498..4578
                     /locus_tag="ACGE4U_000211"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4532..4534,aa:Tyr,seq:gta)
     gene            4585..4656
                     /locus_tag="ACGE4U_000212"
     tRNA            4585..4656
                     /locus_tag="ACGE4U_000212"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4617..4619,aa:Gln,seq:ttg)
     gene            4781..4951
                     /locus_tag="ACGE4U_000213"
     CDS             4781..4951
                     /locus_tag="ACGE4U_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392407.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5171..5878
                     /locus_tag="ACGE4U_000214"
     CDS             5171..5878
                     /locus_tag="ACGE4U_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8392408.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5937..6581)
                     /locus_tag="ACGE4U_000215"
     CDS             complement(5937..6581)
                     /locus_tag="ACGE4U_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFF8392409.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6707..6994
                     /locus_tag="ACGE4U_000216"
     CDS             6707..6994
                     /locus_tag="ACGE4U_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392410.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7102..7422
                     /locus_tag="ACGE4U_000217"
     CDS             7102..7422
                     /locus_tag="ACGE4U_000217"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8392411.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7503..8816
                     /locus_tag="ACGE4U_000218"
     CDS             7503..8816
                     /locus_tag="ACGE4U_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFF8392412.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8813..9532
                     /locus_tag="ACGE4U_000219"
     CDS             8813..9532
                     /locus_tag="ACGE4U_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFF8392413.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9660..10211
                     /gene="raiA"
                     /locus_tag="ACGE4U_000220"
     CDS             9660..10211
                     /gene="raiA"
                     /locus_tag="ACGE4U_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFF8392414.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10433..12967
                     /gene="secA"
                     /locus_tag="ACGE4U_000221"
     CDS             10433..12967
                     /gene="secA"
                     /locus_tag="ACGE4U_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFF8392415.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13093..14194
                     /gene="prfB"
                     /locus_tag="ACGE4U_000222"
     CDS             join(13093..13164,13166..14194)
                     /gene="prfB"
                     /locus_tag="ACGE4U_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFF8392416.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14299..14985
                     /gene="ftsE"
                     /locus_tag="ACGE4U_000223"
     CDS             14299..14985
                     /gene="ftsE"
                     /locus_tag="ACGE4U_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFF8392417.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14978..15862
                     /gene="ftsX"
                     /locus_tag="ACGE4U_000224"
     CDS             14978..15862
                     /gene="ftsX"
                     /locus_tag="ACGE4U_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFF8392418.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16014..17462
                     /gene="cls"
                     /locus_tag="ACGE4U_000225"
     CDS             16014..17462
                     /gene="cls"
                     /locus_tag="ACGE4U_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFF8392419.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17799..18656
                     /locus_tag="ACGE4U_000226"
     CDS             17799..18656
                     /locus_tag="ACGE4U_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFF8392420.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18771..19691
                     /gene="pstC"
                     /locus_tag="ACGE4U_000227"
     CDS             18771..19691
                     /gene="pstC"
                     /locus_tag="ACGE4U_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFF8392421.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19691..20575
                     /gene="pstA"
                     /locus_tag="ACGE4U_000228"
     CDS             19691..20575
                     /gene="pstA"
                     /locus_tag="ACGE4U_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFF8392422.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20590..21396
                     /gene="pstB"
                     /locus_tag="ACGE4U_000229"
     CDS             20590..21396
                     /gene="pstB"
                     /locus_tag="ACGE4U_000229"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFF8392423.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21412..22170
                     /gene="pstB"
                     /locus_tag="ACGE4U_000230"
     CDS             21412..22170
                     /gene="pstB"
                     /locus_tag="ACGE4U_000230"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFF8392424.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22183..22860
                     /gene="phoU"
                     /locus_tag="ACGE4U_000231"
     CDS             22183..22860
                     /gene="phoU"
                     /locus_tag="ACGE4U_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFF8392425.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23437..24129
                     /locus_tag="ACGE4U_000232"
     CDS             23437..24129
                     /locus_tag="ACGE4U_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFF8392426.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24126..24893
                     /locus_tag="ACGE4U_000233"
     CDS             24126..24893
                     /locus_tag="ACGE4U_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFF8392427.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24993..26561
                     /gene="liaX"
                     /locus_tag="ACGE4U_000234"
     CDS             24993..26561
                     /gene="liaX"
                     /locus_tag="ACGE4U_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFF8392428.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26858..27178
                     /locus_tag="ACGE4U_000235"
     CDS             26858..27178
                     /locus_tag="ACGE4U_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFF8392429.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27178..27528
                     /locus_tag="ACGE4U_000236"
     CDS             27178..27528
                     /locus_tag="ACGE4U_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFF8392430.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27695..28633
                     /gene="hprK"
                     /locus_tag="ACGE4U_000237"
     CDS             27695..28633
                     /gene="hprK"
                     /locus_tag="ACGE4U_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFF8392431.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28648..29478
                     /gene="lgt"
                     /locus_tag="ACGE4U_000238"
     CDS             28648..29478
                     /gene="lgt"
                     /locus_tag="ACGE4U_000238"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFF8392432.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29522..30547
                     /locus_tag="ACGE4U_000239"
     CDS             29522..30547
                     /locus_tag="ACGE4U_000239"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFF8392433.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSRAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30562..31500
                     /gene="galU"
                     /locus_tag="ACGE4U_000240"
     CDS             30562..31500
                     /gene="galU"
                     /locus_tag="ACGE4U_000240"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFF8392434.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31625..32065
                     /locus_tag="ACGE4U_000241"
     CDS             31625..32065
                     /locus_tag="ACGE4U_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFF8392435.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32068..32670
                     /locus_tag="ACGE4U_000242"
     CDS             32068..32670
                     /locus_tag="ACGE4U_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFF8392436.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32729..33835)
                     /locus_tag="ACGE4U_000243"
     CDS             complement(32729..33835)
                     /locus_tag="ACGE4U_000243"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFF8392437.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34045..35046
                     /gene="ccpA"
                     /locus_tag="ACGE4U_000244"
     CDS             34045..35046
                     /gene="ccpA"
                     /locus_tag="ACGE4U_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFF8392438.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35383..37728)
                     /locus_tag="ACGE4U_000245"
     CDS             complement(35383..37728)
                     /locus_tag="ACGE4U_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFF8392439.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
BASE COUNT        12315 a         6546 c         8116 g        10849 t
ORIGIN      
        1 ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt tgcatatttt
       61 tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat acttgttttt
      121 ccttttatac attttatggt attatcattt tattagttat tataaaacaa tcgttagaaa
      181 ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga gtcatttggc
      241 gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt ttttcagacc
      301 aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt tagggtacac
      361 tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg ttagcctgta
      421 aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa gattttgagt
      481 aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca tcgtaagagg
      541 tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg ttttgcctat
      601 tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg ttccgattct
      661 ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg tagattttga
      721 tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg aagcgcctta
      781 tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa
      841 tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt
      901 gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga
      961 agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc
     1021 tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt
     1081 agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt
     1141 ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa
     1201 ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac
     1261 acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa
     1321 attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc
     1381 aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat
     1441 gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac
     1501 agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa
     1561 aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata
     1621 tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat
     1681 cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact
     1741 acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac
     1801 agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac
     1861 tttgataaag attttaatgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat
     1921 gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtatttcagc caagtttgtg
     1981 gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc tagtcgatat
     2041 gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt gacgttagtc
     2101 gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt
     2161 tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat
     2221 tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggcgc gctaaaaggg
     2281 acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat
     2341 tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga accagattat
     2401 aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaacaattt ggaaaatagc
     2461 ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt tggattttga acaccgtcaa
     2521 aaaaaaggtt gtgaaaaata cggctagcac tgagcgataa agaaaaagag gaaatttatt
     2581 gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa
     2641 caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca
     2701 agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt
     2761 actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag taaccccaac
     2821 gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca
     2881 actcgcttcc agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt
     2941 acatctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca
     3001 tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag tgattttagc
     3061 aattcactta cctaaaaata gctttaacta ataaaatttc aattcaagaa aatgctttca
     3121 aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg
     3181 aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga
     3241 gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aagcgcaatt
     3301 atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc
     3361 tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat
     3421 tcatggattt aatattttcc gagatttgga aactctgttt caacttccag taacgattga
     3481 aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc gcgattatca
     3541 agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca
     3601 aatcaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct taaatggcga
     3661 taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa
     3721 aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag aaactggaga
     3781 tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag
     3841 tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt ctgcaaaaga
     3901 agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca
     3961 tgatttcgaa ccgcagattt tattatgtga atacagaaac gatgctaatc ttgtaggtgc
     4021 agcagcaaac tatctcgctg tacgtcaagg ggagaattaa gagctttaga gaaggtgcag
     4081 cttgcataaa agaagtgaat gaaatgaata ggagattttt acctaaggaa tgttttattt
     4141 gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa taaagatatt
     4201 gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga agtcactcag
     4261 ttatttatag ggaaaaaaat gattggtgag atcttagcct atggaccaaa agaatatgaa
     4321 attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca
     4381 gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt caataaaggg
     4441 ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa acctttcgga
     4501 ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt
     4561 cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa gggactttga
     4621 ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt aagctctcgt
     4681 tagttcgaga gctctttttt ttgctttgct accttgttcc ttgaagattc tgctgattta
     4741 agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga aagaaaaagt
     4801 agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca atattgaagt
     4861 gaatattccg aaaaaaggcg gaaaagcggt ccgggtaaaa tcaaaacgcc caacgaatca
     4921 tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata agtcggcatt
     4981 ttcatcgatt ttagggtctt ttgttatttt ctaggttgga catatctaaa aaattgtttc
     5041 tgacaatagg agtaggaaga acgatgtgtg tctaaatcct ctgttgatgg atagaaacct
     5101 ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa cgaaaggacg
     5161 ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc aattaaaaaa
     5221 tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa gagaattagc
     5281 agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact tggcagatga
     5341 aggaatctta gaaagaaaga tcggttcggg tacatatgtt gcacgagaaa aagtacaaga
     5401 aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc gagtaccgtc
     5461 tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg aaaaactaaa
     5521 cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag atgatgtccc
     5581 tatttgtttt gaagttgcca gtgtgccaca aaagctgatc gatggctaca gcaaagcaga
     5641 gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga tcggtccagc
     5701 gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt tagaggtaaa
     5761 acgaggagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg gtacgccgtt
     5821 tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg aaaaataaaa
     5881 aacaggtgat ccctgattcc gtattagaaa taaaagagaa tcagaatcac ctgtttttat
     5941 atatgatcag ttaccagttg ctcgtgataa gaaacaggac cctcttcaaa ttggacttgt
     6001 ccgttcagca gctcaatgat ggattcttta aagtggtcag cacttttttc atctacagca
     6061 cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg ttggagttca
     6121 agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat ctcctgttgc
     6181 aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg agtgtatgct
     6241 cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac tactgcagct
     6301 acattgatca gttgattttt tttgaggact tccaacatcg gtatgcccgc cgttccactt
     6361 ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat gaaggcgctg
     6421 cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaact ttttgcttcg
     6481 tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat ctcgatctca
     6541 ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc tcttttttca
     6601 ttttctttat tcagtataaa taaagttcta ggttgtttca acactgtaac ggttgcgcaa
     6661 ttgctgtagt atgcgataat aaagacaaag gcaggtggat ctggggatgc aggaacgaat
     6721 gaaaaagtac gatgcaatta cgcattattt gaagaacaat ggcggctctc aagtaacgtt
     6781 gacctttacc caatttgacg aactgctgtt tcccagtaac ggacttccca aaacagcaag
     6841 ggaaagtaca gattggtggg caaatgatta taaacaccct gaaaaaggtg cctatggctg
     6901 gataaatgca ggctatgaag tagttgtcat caatttggac aaagaatatg tcgtgttcaa
     6961 taaattagtg aaatctagtt ggctatttga ttaaataaac cgcgtttaga aaacgttgac
     7021 aaatttcttt ctttttatat aatagagtaa cagacgcgaa gaaaacgttt gcgtagtttg
     7081 ttttaaaaag gagagaaaag tatggctaaa aaaacaatca tgcttgtatg ttcggctggt
     7141 atgagcacaa gtttactagt aacaaaaatg caaaaagctg ctgaagcaaa aggaatcgac
     7201 tctgatattt tcgctgtgtc cgcttctgac gcagataaca atcttgcaaa taaagacgtg
     7261 gatgtgttgt tattaggccc acaagttcgc tttatgaaag ctgattttga gaaacgtctg
     7321 gaaccaaaag gcattccttt agatgtcatc aatatggctg attatggtat gatgaatggc
     7381 gaaaaagtgt tagatcaagc aatcacgctc ataggtgaat aaatagaaca gtataactat
     7441 cgaacaagcg atccaaaaaa tagatcgctt gttttttcgt atattttttt agggcgaata
     7501 atatgaatca tttttggggt agaaggcttc tgacgagaga tattaaagca atagactgtg
     7561 aggaggtaaa tttaccaaat tatgagaaga tcgcagcagt ggaacgtttg agcaactgga
     7621 ttctttgtca tcgctgtgga atgagaacgt ccgtcattga tggacagctt gcagattacg
     7681 gttatttttg tacacattgt ctatatttag ggcgatgcga cactcaacaa gatttatatt
     7741 tgtttgatca gcctgaagca aaacctcgag aagtcgtctt tttttggaca ggacagctta
     7801 ctgaaaaaca aacggaaatc tcaaagagaa tactttgcca ttcagaaaaa agacatcatc
     7861 ttatttgggc tgtaacaggt gcagggaaaa cagagatgct ttatcctatt ttagtggaaa
     7921 ctttaaaagc tggaggaaga gtagcgattt gtacaccgcg agtcgatgtt tgtaatgaat
     7981 tgtttttacg ctatcgttat gtttttccag aagaaaaaat cacgttgctc catgggaata
     8041 cagcaacggc ttataccttt acttcttttg ttatctgtac gattcatcag ctataccgtt
     8101 tttatcggtc ctttgattta atagtgatcg atgagatcga tgcgtttcct tataacgggg
     8161 atgcaggatt agctcatgct gtgcaaacag cgattaaacc agacggacga ttcatctact
     8221 taagtgccac acctgataaa aagttactaa aggagatcaa gcagacgttt gacatccaca
     8281 aacttgcttt acgctttcat cggcgtttgc ttcctgaacc cgtcttggtt ttcatgaata
     8341 attggcgaca aaaatgcaag aacaagaaga agataaaatc tttggtgcgg atcattcatc
     8401 aattaattaa agaaaaccat atccttttat tttgtccaag tatcacgatg atgcatcgtt
     8461 tgaaagccac acttcaagag gctcttccga gatatgagat cacagatgtt tcttcaagag
     8521 acaatcagcg ggcggaaaaa gtccagaaaa tgcgagaagc aaaatatgac atactgttaa
     8581 ccacgatgat ccttgaacgt ggagtcacat ttgaaagaat atccgttatc gttttaggag
     8641 cggatcatcc ggtttttact aaatcttctt tggtacaaat cgcaggaaga gccgatcgta
     8701 aagggcctta tacgaacagt caagtatatt ttttctatga agaaaaaact gcagctattc
     8761 gaaaagcttg tcaggaaatc aaagaaatga atgaagaagg gaagaagata ctgtgaggtg
     8821 cagttgctgc agtaaagata tcgtccaaaa tttaacattc agcgagatta ttggtgtttc
     8881 gaaatacaga ttacctcaga cttgttcagt ttgtcgcgca caatttcatt tattggaaac
     8941 aagaggctgt tctggctgtc aaaaaccaat ccatttcaat ctagagagta cgaaaaagaa
     9001 agtaaattct atccccgttt actgcagtga ctgtcaagaa tggaagaaga aatatccgac
     9061 ctatgatttt cagcaccgtg ccttttttgt gtacgatgaa aatatgaagg cctggctcaa
     9121 tcaatacaag tttatgggag atatccgact agcaggcacc tttgcatata aatggacgca
     9181 gataaaaaaa gaatacccga catacatcta ttgtccaata cctttatctg aaaaaaggcg
     9241 aacagaaaga ggattcaatc aagtaagcga gatgttgaaa gcagcaagaa ttccttttca
     9301 gatgctactc aaaagagaga agcatctgct ggctcaagca aaaaacacgc gaaaggaaag
     9361 attggagatg cctcaacctt ttgaacttgc agtcaccttt tctaagatac aaaataaaaa
     9421 aatattgatt attgatgatg tctatacaac aggacagact cttttccacg cagcagattg
     9481 tttgcagatc gcacagccga aaatcatccg aactatttct ttggcgagat gaattagatg
     9541 tggaaatttg atagcgattt aatttgcaaa taacctagag ttcgatataa tagaattaag
     9601 aagagggaaa gagtggtcct tctccttctt gattgtggta gacgaaaggg gtaattttta
     9661 tgtttagata taatgtacgc ggcgaaaata tcgaagtaac tgaagcgatt cgcgactacg
     9721 ttgagaaaaa agtaggcaaa ctagaacgct atttcagtga cgcacccgat gcaacggcgc
     9781 atgtgaactt aaaagtttat acagaaaaaa cagcaaaagt ggaagtaacg atcccattac
     9841 cttatcttgt attacgtgcc gaagaaactt ctccagattt atacgcaagt atcgatttgg
     9901 ttgttgacaa attggaacgc caaatccgca aattcaaaac aaaaattaat cgtaaatccc
     9961 gtgaaacggg catgaatact gcaaatgctg ctgctttatt caatgaagag gacgaaaacg
    10021 aaaatgattc tgaattagat atcgtacgta cgaaacgcct ttcattgaaa ccaatggata
    10081 gtgaagaagc agtcttgcaa atgaacatgc tgggacataa cttctttatt tttgaagatg
    10141 cagaaacaaa tggtacaagt atcgtttacc gccgtaaaga tggaaaatac ggactgattg
    10201 aaacagactg atcagcctta cttaaataac aacgaaaact tgcagcagct actgacagct
    10261 gccgcaagtt tttttgagtt tcctcctctt ttagatgaaa tatgaataaa ttatgaagtt
    10321 cttactttaa tacgcttaag atatttcaat tatctataac taatgataaa atgaaaagta
    10381 aagtctacat agtctggata atacacagaa ggaaaaggag attacacaac gcatggccaa
    10441 tttgattcgt tcacttatcg aaaacgataa gaaagaacta aaacgtttag atagcattgc
    10501 aaaaaaagtt gaagcatacg ctgaccaaat ggcagcatta actgatgaac aattacaggc
    10561 aaaaactgct gaattcaagg gcagatacca aaaaggagaa acattagatc aattacttcc
    10621 ggaagctttt gcagtcgtcc gagaagcagc aaaacgtgta ttaggtttat atccatacca
    10681 cgtacaattg atgggtggaa tcgtgcttca cgatggaaat atccctgaaa tgcgtactgg
    10741 tgaaggtaaa accttgaccg caacgatgcc agtatatcta aatgctctga ctggcgaagg
    10801 ggtacacgta gttaccgtca atgaatattt agcaacccgt gactcgacgg aaatgggcga
    10861 actttataat ttcttaggat taacagtggg gttgaatatc aactcaaaat cttcggatga
    10921 aaaacgcgag gcttataatt gtgatatcac ttatagtacg aataatgaat taggatttga
    10981 ctacttgaga gacaatatgg tcgtttaccg ccatcaaatg gtacaacgtc cgctgaatta
    11041 tgccattgtc gatgaagttg actcaatctt gattgatgaa gcgagaacgc cgctgatcat
    11101 ttcagggcaa gcagaaaaat ccacagcttt atatacaaga gcagacaact tcgtgaaacg
    11161 tttgaaagaa gaagaagatt ataaaatcga tgtacaatca aaaacaatca gtttaaccga
    11221 agcagggatc gaaaaagcag aagaaacatt cggtttggat aacttatacg acattgaaaa
    11281 tactgcattg acccatcact tggatcaagc attgagagcc aactatatca tgcttttaga
    11341 tatcgattat gttgtccaag acggaaaagt actgattgtt gaccagttca ctggtcggat
    11401 catggatgga cgccgttatt cagatggact tcaccaagca atcgaagcta aagaaggcgt
    11461 agagatcgaa gacgaaacga aaaccatggc gacgatcact ttccagaact atttccgtat
    11521 gtacaagaaa cttgcgggaa tgactggtac agctaaaaca gaagaagaag aattcagaga
    11581 aatttataac atccaagtat accagattcc gacaaatcgt ccggttatcc gtgatgatcg
    11641 tccagatttg ctttacccaa cattacaaag caagttccac gctgtcgtgc aagatatcaa
    11701 agatcgctac cgcaaaggtc aacctgtgtt agtcggaact gttgcggttg aaacatctga
    11761 gttattatca gatatgctga atcaagaacg tattcctcat gaagtgctga atgcgaaaaa
    11821 tcactttaaa gaagcagaga tcatcatgaa tgctggtcaa aaaggtgctg tgacgattgc
    11881 taccaacatg gctggtcggg gaacagatat caagttagga cttggggtaa gagaacttgg
    11941 tggtctagca gttatcggga cagaacgaca tgaatctcgt cgtatcgata accagcttcg
    12001 aggacgtgca ggacgtcaag gagaccctgg tatgtcacag ttctacttgt cattggaaga
    12061 tgatttgatg aaacgattcg gttcagaacg aatcaaatct ttcttagatc ggatgaagat
    12121 cggtgaggaa gatgctgtta tccaaagcaa gatgctgacg aaacaagtag aatctgcaca
    12181 aaaacgtgta gaaggaaata actatgacac acgtaaaaac gtcttacaat acgatgatgt
    12241 gatgcgtgaa caacgtgaag taatctataa acagcgacaa gaagtaatca tggaagaaaa
    12301 aagcttatcc aagccactaa tgaacatggt caaacggaca atcagtcgtg tagtagatgc
    12361 gcatacacaa ttagagaaag aaaattggaa tttggaaagt atcatcgatt ttgccggcag
    12421 tactcttgtc catgaagata gtattactct tgcagatatc gaaggaaaaa cagctcagga
    12481 gatcaaagag tatctaaacg aacgagcaca agaagtattt aataccaaag cggctcaact
    12541 aaatggtcca gagcaactgt tggaatttga aaaagtcgtg atcttgcgtg ttgtggactc
    12601 taaatggaca gaccatattg atgcaatgga tcaattacgt cagtcaatcg gcttacgtgc
    12661 ttatggacag aacaatccat tagtcgaata tcaaacagaa ggctataaga tgtttgaaga
    12721 tatggtaggc gccatcgagt atgaagtcac tcgaatcttt atgaaagctg agatccgtca
    12781 aaacgtacag cgtgaacagg tttctcaagg aacagcttct cactcagaag acggagatgc
    12841 caaagaaaat agtaatacta gcgcgaaaaa acagccaatc catgtgaaca aggtcggaag
    12901 aaatgatcct tgtccttgtg gaagcgggaa aaaatataag aactgtcatg gaaaaaatgc
    12961 aaactaattt ctgacagcag gattggtgag acaaattctt tttttaagaa ttcgtctcac
    13021 cttctttctg ttatttgctg attctgttag actagatata atgaaaaaag tatagtgaaa
    13081 gaaggaaaaa acatggaaat atccgaaatt cgaaatctcc tgacagaaat gcaggaaaag
    13141 gtcacaagct tcaggaggtc tctttgactt agaccagctg gaagaagaca ttgcggaagc
    13201 agaagcaaaa atggctgaac ctggtttttg ggatgatagc gaagctgctc aaaaagtgat
    13261 cagtgaaaac aatgcaaata aagaaactta tgatcaattc cattctcttg ctgaagaact
    13321 ggaagaaatc gaattgatgg tggagatgtt gcaggaagaa cctgatgcag ccatgcaaga
    13381 agaagcagaa acacgtatca atgcgctaga tgaaaaaatg aagacatatg aattatctat
    13441 gttgttagat ggtccttatg atcgaaacaa cgccatcgtt gaattgcatc caggggctgg
    13501 cggaacggag tcccaagatt ggggcagtat gcttttacgt atgtatacac gctgggcaga
    13561 gcagcatggg tatcaagtag agacactaga ttatcaagca ggtgatgaag caggaatcaa
    13621 gagtgtgacc ctactgatca aaggacacaa cgcatttggt tatctgaaat cagaaaaagg
    13681 tgttcatcgc cttgtacgta tctcgccttt tgactcagcg aaacgacgtc atacttcttt
    13741 ttgttcggta gatgtgatgc cagagcttga tgaaacgatc gatatagaaa tcaatccaga
    13801 tgatttgaaa atcgatacgt accgagcaag tggtgcaggt ggtcagcaca tcaataaaac
    13861 agaatctgct gtacggatca ctcacttgcc tacaggaaca gtagtagcta gtcaggcaca
    13921 acgttcacaa ttgaaaaacc gtgagcaagc gatggggatg ttaaaagcta agctttatca
    13981 attagaagtg gaaaagaaag aacaagaagc agctgcactt cgaggagaac aaaaagaaat
    14041 cggctggggt tcacagatcc gttcttatgt tttccatcca tattctatgg taaaagacca
    14101 tcggacaaac gtagagacag gaaatgtcca agcagttatg gacggtgacc ttgatttatt
    14161 tattgacggc tatttaaaaa tgcatctaca ataagaaccc attgttaaat tgaaataaaa
    14221 ttgtaagatt ttgcaagcat gttgaaacat tgccatgcta taatagctaa agactgaaaa
    14281 agatatggag agaacgccat gattgaaatg aaggatgtaa tgaagaagta ctcaaatggt
    14341 acgaccgcta tccgcaatct ctcaattgtc atcgaacaag gagagtttgt ctatgtggtt
    14401 ggtccgtctg gagccggtaa atctacattc atcaaattga tgtatcgaga agaaaaagct
    14461 tcaaaaggaa ctttgaacgt cgcaggtcat gatctaatag caatcaaaaa ccgcgaagtt
    14521 ccttatctac gcagagaaat cggcgtggtg ttccaagact ataaattatt accaagaaaa
    14581 acagtatatg aaaatgttgc gtacgctatg caagttatcg gcagaaaacc tcgggatatc
    14641 aaaaaacgag taatggaagt gctggacctt gttggtttga aacacaaagt tcgagtcttt
    14701 cctagcgaat tatccggtgg ggaacagcaa cgtgtctcta ttgctcgggc aatagtgaat
    14761 acgcctaaag tacttatcgc ggatgaaccg acaggaaatc tggatccaga aaactcatgg
    14821 gaaatcatga aattattaga tcggatcaat gcccaaggaa ctactgtcgt gatggctaca
    14881 cataacagta cgatcgtaaa tacgattcgg caccgagtaa ttgctatcga aaacggccgc
    14941 attattcgag atcaagcgga aggggaatat ggatacgatg attagaacct ttttccgcca
    15001 tttattagaa agtatcaaga gtttgaaacg taacggctgg atgacagttg cttcagtcag
    15061 tgcggtaacg atcacattga ctttagtcgg tgtattcatg gcagttatca tgaacgcaac
    15121 taaattagct caagatatag aaggaaacgt tgatgtctct gtattcgtag atatcggtac
    15181 aaaagacgaa gagatgaaaa agctagaaaa agaattgaat gacttagatc atgttaaaaa
    15241 agttgaattt tcaagcaaag aacaggaatt gaaaaaaatc caaaacgaaa tgggtgattc
    15301 ctggaaattg tttgaaggtg acaataaccc gttatatgat gtgtatgtag taagtgctac
    15361 tgatccttct tatacaaaac aagtagcaga agaagcagca gatttgaaga atgtttctcg
    15421 ttctgattac ggtggagctt cctcagatcg gattttccaa atcgctggtg cagttcggac
    15481 atggggatta gttgcagcgg cattgctgct gttcgtagcg atgtttctca tttcgaacac
    15541 gattcggatc acgattttgt ctagacagcg tgaaattcaa atcatgcgtt tagtcggtgc
    15601 aaaaaatgga tatattcgct ggccgttttt ccttgaaggt ggctggatcg gtctgatcgg
    15661 tgcgattctt ccgattttga tcatcacatt tggttatgca tggtttttcc aattgatcaa
    15721 tccatcatta ttacgttccc attattcatt gattcatcca caaaatatcg tttggaaaat
    15781 caatctttta atggtcggca ttggtgtgat cattggttca cttggctcga tcatttctat
    15841 gagacgtttc ttgaaattct aataccacaa tataaataaa atgagaaagt tggacagttt
    15901 cgttccagct ttcttttttt attggctagg agaatagaag gaagcggctt ttttcccttt
    15961 actttaaaaa acaacttggg tattcttaag ttggtagaag aagaggtgaa gccgtgaaaa
    16021 ttatcgttga caattttttt acgatccttt tgattatgaa tattttgctt tcctttatca
    16081 tcgtttttcg agaaagaaag gaaactgctc aaacatgggc atgggtgctc gttttgatgt
    16141 tcattccagt tgtaggattt attttatata tttttttagg aagagggatt tccaaagaga
    16201 aaatctttga tttgaagatc caagggaaaa tcggtaagaa cctggaaatt gaagaagaca
    16261 aacaggcatt gatgcgaggg ctttatcctc atccgcctac tggacaagta gatgtgaaac
    16321 aattgatcta tatgctcacg gtatttgaaa gtacgctcta tacgactaaa aatgaaatca
    16381 ctttatttac agatggtcgt gaaaaattcg atgcgctgat tgaagatatc aagcaggcag
    16441 aagaccatat ccacttccag tattatattt atcgcagtga tgcattaggt gaagaagttc
    16501 gagatgcatt gacagaggcg gcaagacgag gggtaaaagt cagagtcctt cttgatgcat
    16561 ggggttccac acaagtttct tccagctttt ttcagaattt gaaaaaagca ggcggagaag
    16621 tcgcattttt ctttcctcta tttgttccgt atattaatcc tcggatcaat tatcgcaatc
    16681 atcgtaaaat cgtagttatt gatgggaaaa tcggatatac aggtgggttc aatgttggaa
    16741 atgaatatct aggattggta aagaaatttg gttattggcg agacaatcat ttgcgtattt
    16801 acggagaagc ggtgtatacc ttgcaaaacc gttttttgat ggactggaat tctcagcata
    16861 caaaagaagt aggctattct cccaaatttt ttccgtccat tcactctact ggtgatatag
    16921 ctgttcaaat cgtgacaagc ggaccagata ccgaacatga acaaatcaaa atgacttatt
    16981 tgaaaatgat ttcgttagcc aaacgggaga tattgatcca aacgccttat tacataccgg
    17041 atggatcgat tcatgaagca ctgaagttgg ctcttctttc aggcgtaaaa gttcatatcc
    17101 aaatacctaa caaaccagat catcttctcg tttactgggc aacgtattct ttcgctgcag
    17161 agctaatcga gtatggagca aggatcgaaa catatgagaa tggatttatt catgcaaaga
    17221 cgatgattat cgatggtggc attgtttcag ttggctcagc aaatatcgat gttcgttcat
    17281 ttcgcttgga ctttgaggtc aatacactgg tttatgatga acgaatggct tcacgtgtaa
    17341 gaaaagcttt ttttgaagac tcgaaaattt ctacgcatct cacaaaagaa atgtatgaaa
    17401 accgaggaat aatcatcaag atgaaagaag gattagcaag attgatttcg ccgctccttt
    17461 aaaacataaa aaggacatga gaaccatcaa cagatggaga tctcgtgtcc ttttctaacg
    17521 gggccatttc tgagagtacg tcagaaatgg agaaagcgtc tcgcatgagc gaaattaact
    17581 ttggggtatc tcttgttttt tggggaaagc aagaaataag ctaatatatg ctattaaaag
    17641 agacacattt caaagccagt atagcataga aacttttaat gagatagttt tttttacaat
    17701 tatgagaatt tattttgtta tttaatgatt ttgattcgtt ttcatcaagt aatgaaaatg
    17761 ataaaataaa catactttag gattaaagga gatttctaat gaaaaaactt aaattattct
    17821 taccaataat aggattagct attcttttat caggctgttc aaagtggatt gatcgaggag
    17881 aatccattac ggctgttggc tcttctgctt tacagccttt agtagaaaca gttgctgaaa
    17941 cttaccagac agaaaatcct ggaaaattca tcaatgtaca agggggcgga agcggaactg
    18001 gtttgtctca agtacagtca ggagcagtcg acatcggaaa ctctgatttg tttgcggaag
    18061 aaaaatcggg gatcgatgcc tcagcattag tcgatcatcg agtagcagtt gttgggatca
    18121 ctcctattgt gaacaaggat attggcgtaa aagacatcag tatggaaaac ctgaaaaaac
    18181 tatttacagg cgagatcaaa aactggagtg aagtcggtgg aaaagatcag ccagtagtta
    18241 tattgaaccg cgcttcagga agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg
    18301 gagaaacagc tgttcaggca caagaacaag attcaagcgg aatggtgcgc cagatcgttg
    18361 ccgacacacc cggtgcaatc agctatgttg ctttttctta tgtttccgat gatgtagata
    18421 cgttgaatgt taacggtgta gaaccgacag atgaaaatgt gacaacgaac aaatggacga
    18481 tttgggcata tgagcatatg tacacaagag atcatccaaa aaaactgaca aaagaatttt
    18541 tagactatat cttatctgat gaagtacaag ataacatcgt tggagaacta ggttatatcc
    18601 ctgtttctca tatgaaagtc gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt
    18661 tacacaaaat ttacaaactt agctaaactt ttacatgtta ttaaaaccaa attgatgttc
    18721 aatttgtaca ctgagtgagt atgttaaacc tgattaaggg ggagtcatct gtggaagatg
    18781 tgaaaaaagt attattaaca aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca
    18841 tcagtttttt atgtatcgcg ctgatcgtgt tggttgtatt atctattttt tattttgtag
    18901 ctagtaaagg attggccacc ttttttactg ataagatcaa tgtatttgat tttctgtttg
    18961 gtacagattg gaatccaagc caaatcggag caaatgggaa accaatggta ggggcattgc
    19021 ctatgatcac aggttcgttt attgtgacct ttttatctgc tatcgttgcc acgccatttg
    19081 ccattggtgc agcagtattc atgacggaga tctctcctaa aaacggaacg aagatcttac
    19141 agccggtcat cgagctgctt gtaggtattc cttctgtcgt ttatggtttt atcggtttat
    19201 cagttatcgt gccttttgtc agaagtattt ttggcgggac tgggtttggt atcttagctg
    19261 gtacatttgt gttgttcgtt atgatcttgc cgactatcac aacgatgact gttgatgcat
    19321 taaaggcagt gccacggcac tatcgggaag cttcattagc gcttggtgcc acaagatggc
    19381 aaacaatcta caaagtcgtg ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt
    19441 ttggtatggc tcgtgcgttt ggtgaagcct tagctatcca aatggtcatc ggtaatgcag
    19501 cattgatgcc aacaggtctt actactcctg cttctacatt gacgtccatc ttaacaatgg
    19561 gaatcggaaa tacaatcatg ggaacagttg aaaataatgt gttatggtca ttagcgctga
    19621 tcttgctatt gatgtcatta ttctttaata tcgtgatccg tatgattggg aagaaaggag
    19681 agctgaaata atgaacgcaa aacgctcaga taaaattgct acaggtgttt tatatatagt
    19741 ttcggggatc atcgtattga ttttagcagc tttactactt tatatcttag ttcggggaat
    19801 accgcacatt tcatggtcct tcttgaccga accttccaaa gcctaccaag aaggcggtgg
    19861 aatcgggatc cagctattta actcgattta tttgcttttg atcacgatga tcatcagttt
    19921 tcctatttct ttaggtgcgg gtatctatct ttcagaatat gctggaaaaa actggctaac
    19981 agatgtgatt cgcacgtcga ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt
    20041 attcggtttc ttggttttcg ttattcaatt ccagtatggt ttctcgatta tttctggtgc
    20101 attggctttg actgtattca acttgcctct tttgactaga acagttgagg agtctttaca
    20161 agcggtccat tacacacaac gagaagcagg attggcctta ggtttgtcac gctgggaaac
    20221 agtgataaaa gtggtggttc cagaagcgac accagggatt ttgactggtg tgatcctaag
    20281 ttctggaaga atttttggtg aagcagctgc attgatctat acggctggac aaagtgcacc
    20341 tgcgttagat tttacgaact ggaatccatt aagtgtttca agcccgatca gcattttccg
    20401 tcaagctgaa acattagctg tccatatttg gaagatcaat acagaaggca cgatgcctga
    20461 tggtgtttct gtttcggctg gcgcttctgc cgtgttgatt attgctgttt tactgttcaa
    20521 cttcggcgct cgtgcgattg gcaaacgatt ataccgaaaa atgacttcag cctaaggaga
    20581 agaagcaaaa tgaaaaaata taactggaat gaaacaaatg tcatcaccat gccaaaagag
    20641 aaagaagtag cactatatac ggatgatcta catgtctggt atggcgacaa tgaagcaatc
    20701 aaaggagtgg atctggaatt tgaaaaaaat aagatcactg cactgattgg tccttccggc
    20761 tgcggaaaat ctacgtatct tcgttcgttg aatcgaatga atgatgggat cgctaatacg
    20821 aaaatcactg ggaaaatcat gtatcaaggc gtagatatta atgcgcctca agtggatgtt
    20881 tatgagatgc gcaaacggat tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg
    20941 atttatgaca atattacatt tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta
    21001 gatgaaatcg ttgaaacaag cttgaaacaa gctgccttgt gggaccaagt aaaagacagc
    21061 ttggacaaaa gtgccttggc tttatctggc gggcaacagc aacgtttatg tattgctcgt
    21121 gcgatcgcga tgaaacctga tatcttgctg ctagacgagc cggcaagtgc tttggaccct
    21181 atttcgacag gaacggttga agaaacactt gtaaacttga aagaagacta tacgatcatc
    21241 atcgtgacgc ataacatgca acaagcagct cgtatcagcg actatactgc ctttttctat
    21301 ttaggaaaag taatggaata tgacaaaact aacaagatat ttaccagacc aaaaattcag
    21361 gcaaccgaag attatgtgtc cggtcatttt ggttaagacg gaggcctcaa catgacaaaa
    21421 gagattattt catctaaaga tcttcatctg tattatggaa agaaagaagc actaaaaggg
    21481 atcgatctaa gcttcaatca aggggaaatc actgcgatga tcggtccttc tggttgcgga
    21541 aaatccactt atctgcgttc tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc
    21601 accggaagtg ttttatataa agacaaagac atctatggtc caaaaacaga tgttgttgaa
    21661 ctaagaaaag aaatcggcat ggtattccag cagccaaacc catttccttt ttctatctat
    21721 gaaaatgtca tctatggatt gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa
    21781 gtagttgaag aaagcctgaa ggcagcgtct gtttgggatg atgtgaaaga caaattgcat
    21841 aaaagtgctt tatctttatc cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg
    21901 gcagttaatc cggaaatcat cttattggat gaaccaacaa gtgcgcttga tcctgtttct
    21961 actgggaaaa tcgaaagtat gctgttagaa ttaagagagc ggtatacgat gatcatcgtg
    22021 acccataata tgtctcaagc ttcacgtatt tcagataaga cggcattctt tttagatggt
    22081 cacttgatcg aatacaatga tacgaagaag atattcatga atccagaaaa acaagagaca
    22141 gaagattata tttcaggtcg atttggataa ggaggaaacg gaatgctacg tacacaattt
    22201 gaagaagaat tgctgaatct ccataatcag ttctacgaaa tggggatgat ggtgagcagc
    22261 gccgtgcaca agtcagttcg tgcatacatc aaacacgata aaatcttagc ccaagaagtt
    22321 atcgaaaacg atataaatat caataacatg gaaacacgct tggagaagaa aagttttgaa
    22381 atgatcgcat tgcaacagcc agttacaact gatttgcgta tgatcattac ggtcatgaaa
    22441 gctagctcgg atttagaacg tatggccgat catgcggtgt ctgttgctaa atccacgatt
    22501 cgcgtaaaag ggcagacgag aataccggaa atcgaaaaag agatctccga tatgtccgat
    22561 tatgtgaaaa aaatggtgga caacgtccta gttgcttatg tcaaaacaga cgaaaaagac
    22621 gcacgtacga tcgccaatat ggatcaaaga gtcaatgact atttcaatcg aatctataat
    22681 gcaacaatca aaaatatgca ggaaaatcct gagacagtca tcagtggaac agactacttg
    22741 aatgttgcca gttatctaga acggatcgga gactatgtga caaacatctg tgaatggatc
    22801 gtctacttgg ctacagggaa aatcactgag ttaaatacga accacaatga gattttctaa
    22861 actgaagctg tgattatgcc gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga
    22921 aaaagctgtt ctgcatcaag taataagaac agtcaaacaa aaaatagctc ctcatatttt
    22981 tcgtttggct ggacttatta ccgcagatgc aagcttttga acaccgttta ttcgggaaag
    23041 ggtttgtgac aaaacatgtc acaaaccctt ttcatcattt catagaaagg ataaagaatg
    23101 caaaaaaaaa ttagcatcat cactatattt tttatttgtg tgcttacttc atgggttatt
    23161 cagcatcaag ccgctttata tgaagtacct gtcatcgaga tagaaaaagt acgaataaaa
    23221 gaggatcatc aattgattac aggaaaactg atgaacacgg atcaaaaagg agaatcgatt
    23281 tatttatcgg ttccttatca aaaaaatcaa atagaaacag caaatataaa agcgagacaa
    23341 atgtatctag tccaaatgca tggagatgaa tggcagctag tagcaaaaaa aaatgatggc
    23401 tggcttttct tcttcacaag tatcttcatt ggcgccatgt tgcttattgg tggaaaacag
    23461 ggtctatttg ctttaggaag tctcatcatc aatatcgttt tacttttact tttgttgctc
    23521 atctttacta aaacagaagg gatttctcta ttaaatatat ctatcctgtt cgtcgttata
    23581 gggatgatta ttagcatatt ggctttagat ggatggaata gaagcagtat aatcaaaatt
    23641 tcagctgcag ttacgagtgt ttttttagca ttttttattt gtcttttgac aatgaatcat
    23701 tggaaagacc aaggactgcg ctatgaggaa ctgaattatc tgacacgacc gtatcgttct
    23761 gtttttcttt ctagcgtact gattggaaca gctggaggaa cactcgatac cgttatcaca
    23821 gtcattgcga cattagaaga attgactaag cgacaaccag aaataacagc gaaacagtta
    23881 tggctttcag gaaggcaagt gggtcgagat gtgattggct cgatgaccaa tatccttttg
    23941 ttcgtctata tcagtgggtc agttccatcg cttcttttat atttaggaaa tcaatggtcg
    24001 ttcaaagaaa cgatagaaca gcatttatca ttggaatttt tacgcgtcat tgctggcagc
    24061 ttaggaatag tattatcgat ccctatctcg gtcctctttt ttctgactgt aaggaggctg
    24121 aaacgatgaa tgtacttatt atacttgttg ggattttagc aatcagcgtg ctacttattg
    24181 gcggtacgca aggtatgtat attcttttag gcttattcat caatttaggg atctttttcc
    24241 tgttgttatt cggttatcat cagaagtggc cgatactggc attgtcggtc ataggatttt
    24301 tattgattgc tgtggttatc ttatttttta tcaacggtta taatctaaaa atgcgtgcag
    24361 cttttgtgag tatactgatc tttttatttt gtttcctact attgcttcct atcactgatt
    24421 ttttagcaat ccaaggtttt acatcggtag aactagaaga attatctggc ttagataaga
    24481 ctttagcgat tgattttaga ttacttacga gatcgttgct tctgattagt cttagtggtg
    24541 ctgttttaga tgcgagtgta gcaatttcta gtgggacatt cgaagtatat caggcaaatc
    24601 cgcaattatc tttttaccaa ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg
    24661 cgtcaacagt aatgacactt ctttttgcat tcatgggaag ttctttagcg ttgatcctct
    24721 ggtttattga tttagacatt ccttttcagc aagtgatcaa tgaaaaatca tttgttctag
    24781 aatatacgat ggctattctt accacattgt ccgcactact tgttcttcct ttaacctgcg
    24841 tgactacagc atatctcttt acaaaaaaga aagaacaact caagatggag taaaagttac
    24901 aacttaagtc ctatgacatt tttactgaat cagtgcataa tatggatgta aggaaaagct
    24961 tgcaaacaat tttgcttaag gaggcaattt ccatgaaaga aagagaacgt attttagatt
    25021 tagtgaaaaa aggaatcctt tcgactgaag aaggcttaga tttattagaa agcatggcaa
    25081 ctgaaaaaga cgaaaagcaa atcaaaaaag aagcagacaa ggtaactgct agtcataaag
    25141 aaaaagatca ggcaagtcaa ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg
    25201 agccagttga tccaaaggaa agagaaagac aagatcaaga aaacttagaa aagatcttag
    25261 atgaactagc aactgaagcg aataaaactt cggcgcgtct agatgaagta aatgctcaat
    25321 tcgctgacaa taaggcagca cgcaatgaga aacaggaagc attgatgcag ctgaatacaa
    25381 aagaagaatt aggcgagttg acagaagaag aattagcaca gcgtcagacg ttagaagcag
    25441 aaatcaaaga attagaagct agtggcgata cactgttaga agaacaattg aaattagaag
    25501 cagaattaaa agatatccgt aagaatcaat ggtctgagaa aaaagaaacg tttactgata
    25561 agtttgaatt gccagatgat tggaaagatc aagcaacaga tacactgaat caagtaggcg
    25621 aaaaaatgtc agaagctgga tcacagttag gcaaattctt gaagaaaaca ttccaaactg
    25681 tttcagaaac agtgaatgac aacatggaat ggaaagatgt cagcttacgt gtccctggga
    25741 ttgcgacaac gaaatttgag catgaatttt actatgaagc tcctgcagca agtatccttg
    25801 atatcaaggc agcaaacgga aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag
    25861 ttgaagctaa gatcaaactt tacggaaaaa tgggtgctga accatttgaa gcattctctg
    25921 aacggagcca gattgaggtt aatgaagatc atatctcctt ccaaattcca aataaacgtg
    25981 tgcgtgcaga ccttgttttc tacttaccta aacgagtata cgaccacgca gctattaaac
    26041 ttctaaatgg caacatcatg attgaaacat tggaagctaa agatatttat acaaaatcta
    26101 cgaatggtaa catcatcgtg gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa
    26161 acggcaatat cgatattcga aacgggaaca tcttagattc tattatcgaa acagtaaatg
    26221 gtacagtgac atttggtgcg acagcagaaa acctttctgt gtctttggta aatggtgatg
    26281 tacgtcttac gattaaagaa gataatttga aaaaagtaga agcaagttca gtgaacggaa
    26341 atgtcaaagt cgctttacca gatggcatcg gtttggaagg acatgctaaa acaagtcttg
    26401 gaagtatcaa cagccgtctg tccaattacg aagtagttcg tgagaaaaaa gaacggacaa
    26461 accaaatgtt gcaattccgt cgtgtttcag atggtgaaat cgcgcaagtg caactttcaa
    26521 cgacaacagg aagtatctat ttaaaagata cagataaata aagaggttgt gaaagagatg
    26581 ttcagccttg agcaataaga agcgattttg taaaatagct tcccctattt tacaaaattg
    26641 tgccttattg ccgatagtct atctcttgaa caccgtttac caagagatta tgaacctgcc
    26701 aattatcgcc gagtcacaag gaataatttc agaaaaagct cgtcacgttt tatgaatatg
    26761 gtaatttgtg accgagaatt ttgcagaaag acaccgatta agaagaaggt tgtcacaacg
    26821 attttcttca tttaaaatag tagaacagag gtgagacatg atgaaaaaaa agttgacaaa
    26881 atctaataaa aatgttgttt tgacagggac tttagcagga ataggagaat acatcgggat
    26941 tgatccgact gttatccgtg tgctttatgt attcctgagc ttagttgctt ttggaagtcc
    27001 aattatttta tacattttat tagctttgat tatcccatct gcgcgttctg cacgaacaaa
    27061 ctatggacat aacaatgcat attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca
    27121 aacaaaaaaa cgcaaagaag cggaaaaaat cgatgatgac gaatggagtg acttttaatg
    27181 tcgtactttc aacgtatttt agttaatacg ttaacattca tatcattggc tgttcttttt
    27241 cctgcgatgg tccatgtgag cagtatttgg acggccgttc ttgccagctt cgtattgtct
    27301 gtgttgaata tgtttgtcaa accgattttg acgatactgt ccctgccctt tacattagtc
    27361 acattgggat tgtttacgtt tgttatcaat gcagcgattc ttaaaatgac ctcctttttt
    27421 gtgggagcag aacattttag tttttcttca ttcggtgcag cgttgttagt agctatcttg
    27481 atgtcgcttg tcaatgtaat tgtcagcgaa cataacagta gaaaataaaa gaaagctgtg
    27541 ccgaactatc ggcgcagttt ttttatttag aatcaggaat gcgttttcta ttacattggt
    27601 aattaaataa aaacgttttt tttaattggt agtacttttg gctagacaat gataaaatga
    27661 tagagaacac tctaatagaa atgaggtagc atgaatgtca gaagttgtaa aagttagcca
    27721 attagtgaaa aaattatctt tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt
    27781 catcgtaact ggggacatct cacgcccagg actagagttg actggttatt ttaattatta
    27841 ttcccataat cgtttacagc tatttggcag taaagaaatc agttttgcgg aacggatgat
    27901 cccagaagag cgtttgatca tcatgagaag attatgtagt gaagatatgc ccgcatttat
    27961 tatttcaaga ggattaccag ctccagaaga aatgatccag gcagcaaata aaaatgggat
    28021 agctgtactg cgttctccca tctcaacttc tcgtttatta ggagaattat ccagttattt
    28081 agatggtcgc cttgcccctc gaacaagtgt ccacggtgtt ttagtggatg tttacggatt
    28141 aggtgtactg attcaaggtg acagtgggat aggaaaaagt gaaacagcgt tggagttgat
    28201 caaacgcggc catcgattga tcgcagacga tcgagtagat gtttatcagc aagatgaatt
    28261 aacggtgatc ggagaaccgc ctaaaatcct ggaacatctg attgaaatcc gaggaatcgg
    28321 gatcattgat gtaatgaatc tttttggtgc gagtgcggta cgtggctcta tgcaagtcca
    28381 gttggctgtt tatctggaag catgggcaaa agataagaaa tacgaccgtc tgggctctga
    28441 agacacgacc gttgaaatcg cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac
    28501 aggacgaaac gttgcaatca tcattgaagt agccgcgatg aatttccgtg cacgtacaat
    28561 gggctttgac gcaacgaaaa catttgaaga aagattaagt cgcttgattg aagaaaactc
    28621 aggcaacgac tagcaaggag tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag
    28681 cttgtaggga ttcctatcta ctggtatgca ttgatcatcg tatcaggtat cattatcgct
    28741 atgtggctta gcagcagaga ggctgttcgg gtaggattaa aagcagatga tgtgacagat
    28801 tttatgttgt ggggattgcc tatctccatt attggcgctc gcttatatta tgttttattt
    28861 gatcttcccc agtatatagc tgatccaatc caaatattca atatccgttc aggaggctta
    28921 gctatttatg gcggactgat tgcaggcgcg atcacgctgt atttttttac taaatatcat
    28981 tttatttctt tgtggacttt tttagatatc gctgcaccaa gtgtattatt agcacaggca
    29041 atcggtcgtt ggggaaattt catgaaccac gaagcatatg gaccagacac gacgcgaagc
    29101 ttcttagaaa gtctgcattt accgcaattc atcattgata atatgtatat cgaagggatc
    29161 tatcgtcaac caacatttct ttatgaatca ctttggagtt tgattggatt tattttgctt
    29221 ttgtttttac gaaagaaacc acaccttttg aaaaaaggcg aagtcctttt atgttatcta
    29281 atgtggtata gtttcgggcg tttctttatt gaaggattac gtacagacag tctttacttt
    29341 ttaggtgtga tccgtatttc tcaattatta tctgctgtgt tatttgtcgg tacattgatt
    29401 ctatttattt ggcgcagaaa aaaaggaaat ttaccggatt atgaccgctc attaggaaaa
    29461 aatcaacaaa ttatctgatt tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa
    29521 aatgaattat agagtagcgg ttttaggacc aggctcttgg ggtactgctt tggcacaagt
    29581 cctcgccgaa aacggacatg acgttcggat ttggggacat cgtgcttcac aagtagaaga
    29641 aatcaacacc caacacacga atcaacgtta tttaccagat cattatctac ccgattccat
    29701 ccaagcattc accaatatgg aagatgcagt aaaagacgca gatgcggtgc tattcgtcat
    29761 tccaacgaaa gcaatacgtt ctgttgcaaa agaactagtg cctaaattga atacaaagcc
    29821 agtgatcatc catgcaagta aaggattaga acagggaaca cataaaagga tctctgacat
    29881 cttgttagag gaaatcccag cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag
    29941 ccgtgcagaa gaagtagcaa aacatgacat cactacgatc acagctgcaa gtacagatgc
    30001 aaaagcagca aaatatgtac aagagctgtt catgaataat tattttcgga tctataccaa
    30061 tcctgatgtg atcggtgtag agatgggggc agctttgaaa aatattattg ctattggtgc
    30121 tggcgctatt cacggcttag ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg
    30181 attagctgag atcagccgtt taggtgtagc aatgggtgcc aacccgctga cctttatcgg
    30241 attaagcgga ataggcgatc tagtcgttac ttgcacaagt gttcattcta ggaactggcg
    30301 cgcaggcaac ttgttaggac aagggcagcc cctagaagag gtgctagcca atatgggaat
    30361 ggtcgttgaa ggtgtgaata ctacccaagc agcgatggaa ctagcccaaa tgatggaaat
    30421 cgaaatgccc atcactcaaa caatctatga agtgctgtat gaacaaaaag aaatcaaaac
    30481 agccgcaaaa gaaatcatgt tacgtgatgg aaaaaaagaa aatgaattct cattaacaat
    30541 tttttgaagg agcgaatgta tatgaaagtt aaaaaagcag ttattccagc agcaggatta
    30601 ggaacaagat ttttaccagc aacaaaagct atggcaaagg aaatgttgcc aattgttgat
    30661 aaaccaacaa tccagttcat cgttgaagaa gcgttgaaat caggtatcga agacatcttg
    30721 atcgttacag gaaaagctaa acgaccaatc gaagatcact ttgattcaaa tattgaatta
    30781 gaaacaaacc taagtgaaaa aggaaagacc gaattattga aattagtaga agaaacgaca
    30841 gatgtcaact tgcatttcat ccgtcaatct catccaaagg gtttaggaca tgccgttctt
    30901 caagcaagag cctttattgg aaatgaacca tttgtcgtta tgctaggcga tgatataatg
    30961 gaagacgaag taccattgac acagcaatta atcgatgatt acgaacgtac tcatgcttct
    31021 acgatcgccg ttatgcaagt tcctcatgat gaaacttcaa aatacggaat tatcaatcca
    31081 ggagaagtat tggaagatgg gttatacaat gtcaaaaact ttgttgaaaa accagatcca
    31141 agcaaagcac caagtgattt agctattatt ggacgttact tattgactcc agaaattttt
    31201 gatgtattgt ccgagcaaca accaggagcc ggaaatgaaa tccaactaac tgacgcaatc
    31261 gatacattga ataaaacaca acgcgtattt gcccgtaaat tcacaggtaa acgttacgat
    31321 gtcggtgaca aatttggttt tatgaaaaca agtattgaat atggtctaac acatccagaa
    31381 gtaggtgcac ctttacgtga gtacatcctg tctttaggta aaacattaga agcagaagga
    31441 agcaagatcg atcataatcc aaaaggaata acagaaatca aagaagaaaa gaaaaaataa
    31501 cccttttttc taacgagctt cagctgctaa ttgaataaaa agtagttgaa gctcgttttt
    31561 atttgtttaa aaaacacgaa aatgctatcc ttatagaaaa gagagacaag aagggagttt
    31621 gatcatgtca ggaggagaaa ttgcaggatt gatcgcagca atcgcctttg cgatacttgt
    31681 cgtttttatc gttcgtgtgt tgttgcaagt ttctaaaact ttagaaaaag tagatcagac
    31741 tgtagcagaa gcaaatacga cgatcgaaat cgtcacaaaa gacgtagatc ttttatctag
    31801 acaagtagaa ggcttactgg tcaaaagtaa tgaactgctc ttggatatca atggaaaagt
    31861 cgcaacgatc gatccattgt tcacagcagt tgctgattta agtgaaagtg tatcagaact
    31921 taatcattct agtaaaaaca tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa
    31981 agcgacggtt gccggaaaag tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca
    32041 tactgaaacg acaggaggaa aataaaaatg gctaaaaaag gtggattttt attaggtgca
    32101 gtagtcggag gcacagccgc agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa
    32161 gaacttcggg atgaattagc tgcccaagcg gatgatttga tggatatggc ttcagattac
    32221 acagatatgg cgaaagaaaa atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa
    32281 gatctttctc aacaagcagg tgatctagca ggtagtgtca aacaaaaagt aagtgaatca
    32341 gcagatgaag caggaagtga atctgacgat atcttgttaa atcttaaaga acaatcagct
    32401 gacttatcta atcgtttcaa aaaaacggca gaagatatga aaaaacctgc agatgaatta
    32461 ggaagtattg ctaaagatac ttcagaagat atcatgattg atgtaaaaga cgctgctggt
    32521 aaagcaggag atactgtcaa tgaaggaatg tcagaagcaa aaccagttgc agatgactca
    32581 aaagaaatga tgaaaatggc aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat
    32641 acaatgacag attcatctac aaatcactaa aatcacaaat ttatagaata aaaaggtggg
    32701 agcggactag ctctcacctt ttttactctt atttttctaa aacaagtaat tcttttggtg
    32761 tagtcgtgaa tggcacacag ccatcgcttg tcacatatac acagtcttcg atacgtaccc
    32821 ctactttttc aggaatatag attcctggtt caagagagaa gcacatccct tcttcgatta
    32881 ctaagtcatt tccttctaca agggaaggga attcgtggac ggttgttccg atcccgtgac
    32941 ctaaacggtg attgaaatat tcgccgtagc catagctgtt gatcacgttt cgtgcgattt
    33001 gatcaagttc acctgctgtc acacctggac ggacggcttc ttgtgcggca agctgtgctt
    33061 caagagtgat gttgtagatt ttttcttgga agtcggttgg tttttgataa gcaattgtac
    33121 gagtagcatc actgcaatag ccattccaaa caacgccaag atcgaataac acaagttcat
    33181 tcggtgaaat cttcgtattt cctggtgtac catgagggct tgcagcattt gctcctgcaa
    33241 ggactaatgt gtcgaaagac atttgtgaaa caccttgttt tttcaattgg tattcaattt
    33301 ctgcaatgat cgcttgttca gtcacgcctt ctttcactgc tttaaagcca atctcaaatg
    33361 caacatcggc ccatttacca gcttcgatca gtttgtcttt ttcctctaga gttttgatca
    33421 gttgtagacg ttggatgatg gaagttagat cagtgtaaga atccgttccc gtcaagatat
    33481 cagagatagc atcgaagcgc tcgacagtca aggcgttctt ttcaatccct acattgccag
    33541 gctgaccata gcgagcacga agttcttttc cgatgatctc ccaagggttt tcgctgtcta
    33601 aatagccacg gacatcatac gcccaactac tgttttgagc atcttcaact tccaaagcag
    33661 gagtgaaaag gaaaggatct tgttcaagag ggataaaaag agctagtaca cgctcatgag
    33721 gatcactttc gtatccggaa aagtaagcga tgtggccagg gtcgctgata taagccaatt
    33781 cgacttggtg ttgtgccatc cattgacgta gttcattgat ttttacgtta ttcatttggc
    33841 aacctacttt ctaaattaat tttgtattca ataattgtac cactgattta ttaaaagacc
    33901 aacgttctaa aaactgaaaa aagtaaaaat tttcatgaaa acagagataa aacggttgca
    33961 attcttgaga ttatttgata ttctatttta gaaatgtaag acgtattttc agaaaacata
    34021 taaaatatga ataggagaac acaaatggaa aagcaaacaa tcactatcta tgatgtagcg
    34081 agagaggcaa atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa tccaaacgtt
    34141 aaaccagcaa caagaaaaaa agtcttggaa gttatcgatc gcttggatta tcgtccaaac
    34201 gcagttgcgc gtggcttggc aagtaagaaa acaacaacag ttggtgtaat catccctgat
    34261 gtcagcaata tgttctttgc ttctttagca cgtggtatcg atgatgtggc aacaatgtac
    34321 aaatacaata ttatcttagc aaattcagat ggcaacgacc aaaaagaagt caatgtattg
    34381 aataacttac tggctaagca agtggacggc gtgatcttca tgggtcacca tatcacagac
    34441 gaaatccgtg gagaattttc acgttcaaaa actcctgttg tattagctgg ctctattgat
    34501 ccagatgaac aagtaggtag cgtaaacatt gattatgtat ccgcaacaaa agatgcagta
    34561 aacaaattgg caaaaagcgg caataaaaaa atcgcttttg tcagtggtgc gttgatcgat
    34621 cccatcaatg gacaaaatcg tctgaaaggc tataaagaag cattaagcga aaataaccta
    34681 ccatatagcg aaggattgat ctttgaagca caatacaact tcaaagatgg tctatctcta
    34741 gtcgatcgga tccataatag cggtgcaaca gctgtctatg tttcagatga tgaattagcc
    34801 atcggtattt tggatggttt attagaccgt ggcgtgaaag taccggaaga attcgaaatc
    34861 atcacaagta acaattcact actgacagaa gtagctcgtc ctcgtttaac aagtatcaca
    34921 caaccattat acgatttagg ggcggtatct atgcgcctat tgacgaaaat gatgaataaa
    34981 gaagaagtcg aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa aggatctaca
    35041 aaataataaa aggacaacca ttcagaggtt gtgtgcctgc cgtttaactc tgcgttacaa
    35101 ggagcaattt caaaaaacag catctcatgt ttgatgaata ttgtgactcg tgattcggag
    35161 ttggcaggag aacaccgttt atttgggaaa aggggttatg acaaaatttt gttataatcc
    35221 tttttttgtg tcttaaaaaa gagagaaagt ggttgaaatt acatttatcg tatataaaac
    35281 aatccgtgta tagatgctgc attttgttca ccaccatcaa aaaaataagg gactgtgaca
    35341 atactattgt cacaatccct tattactgtg tattctatta aatcaatcat ctttctcatc
    35401 tgattcttta gttgcttgat tcgctcttgc ataagctgaa gcggtgttcc agtaatctgt
    35461 atagttttca tcggtaccgc caacgccaaa tttaaaggcg cttttcttcg gtccattttt
    35521 cgcccatagc gattcaactg tttttcctgg tgtttggatg gattttttat tgacagtaac
    35581 ttttccaggt gtttgaccag taaatccaga tacttttttc ttcatcacat cagatgataa
    35641 ttcgaatttc tcatcagtgc caaaaatgtc aggttttgct tggtaagctt gattgatcag
    35701 attggcaaga taattagccg aacgtatcct tgctttgctg tccattccag tattgtcatc
    35761 gtgccctgcc cagctactga tggtaataga cggagtagag acaattagcc atgaatctcg
    35821 gtattcattg gtagaaccag tttttccgac ccaatctgct tttcctagat ttccgtttaa
    35881 gcttgagatg gcatctttga atggtgtagt gatttttgca ttgatcacac tacgcatcat
    35941 gtcattcatg atcgatgccg ttgcttccga atagatccgg acagagttgc tttcgtgttt
    36001 ataaattaca tttcctgcgt tatctgtgat ggaatcgatg atatagcctt cttcatagac
    36061 gccgccgttt gctaaagcct gaaatccgtt ggtctgttga agcgtcgtga catctactgt
    36121 acctaaagga gcagattcaa cgccccaatt gtcatttgcc gggtagttca tctttgccaa
    36181 gtagtgctcg taagcatatt gtttcgatcc accggaattc aacacatctt gatataagtg
    36241 ataagccggg atattgttgg accattctag tgactcacgg acggtttgga aggtgttcga
    36301 tccttcgtta gtagcattga cgattttctc gccagcattt tctccttctt gccaagttgt
    36361 tgcataatct gatacacggg attctgatcc gataagcccc atgtcgattg caggtccata
    36421 aacaagaact ggtttgattg atgagccagc ttgacgttct gtatcgaagg catggttatt
    36481 ttgattctga ctgtaattcc gtccgccaac gaatccgtag atgcggccag ttctgttatc
    36541 catcaataca tttccggttt ctacttgact gccagcacca tcatctaaca tgtatccata
    36601 attttcaacg ccattttgca atgcagcata gacatcttta tcgattgtcg aatggattgt
    36661 atatccttta ttttggatcg tttgctgtgc cttttgataa taagcgtcac tgaccgatga
    36721 atcagacata tcagcgttgt ctttttcagc caattgttgt gcaatgattc ttgttgcttc
    36781 attcatcaca gtataataca agaattctct gtcagtctgt tctgcgattt gttgtggtag
    36841 aaagtctttt gtaaggtcat aggcttttgc ttcttcgtat tgttctttcg taatttggcc
    36901 ttcacgatac atactgaata aaacaaaatc ttttctggca aggccggcag aaagatcttc
    36961 ttttagcgcg cctgtgttcg tgtatggact atacgtgatc gggctttggg gtaatccagc
    37021 gatataagca gcttgcggca gcgtcacatc ttttgcatct acaccaaaga ttccttttgc
    37081 agcttcctgg actccagcaa tgttttgtcc cttattatta cgaccaaatg gtgagacatt
    37141 caaatacgta gcaatgatct catcttttga aaagtatttt tctacttgtg cagaaagaat
    37201 gatctcgttt gctttacgtt tgaatgttgt ttcatctgta aggatctgct gtttgactaa
    37261 ttgctgtgtc aaagttgatc caccagaaga gccaatacca gtagcttcag atactaatgc
    37321 tcgtaaaaca gctttaggaa cgtaaccttg gtgtttatcg aagtattcat cctctgtgct
    37381 gataattgca gttttcaata aaggtgaaat cttatcagat gaaatagtcg tgcgcatcag
    37441 atctgtctgg atttttgata tctctgtatt atctgcataa acaatctttg acgtttctgt
    37501 aatgtttcct agttctgtct gcagtgcttt tttagtcggc aattgtgtgt cttcgacgag
    37561 ataggcaaaa tagccagcgc ctattccaat acctagagct gcgaacatca aaagaagaac
    37621 agtaataaaa acagtcaatg attgaaaaac tctaaaaata atcttaggaa caaaccattt
    37681 ttcttttttt cttctattct gtttttttcg agtatttttg cgtggcaaaa gggtcacctc
    37741 aactaatggt tttatttcgc taattatagc aaataataga acaaaaaaac agacaaggct
    37801 gtttttggca ggaatttctt ttttaa
//
LOCUS       DAXWLF010000007        36437 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXWLF010000007 DAXWLF010000000
VERSION     DAXWLF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.007"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(195..773)
                     /locus_tag="ACGE4U_000246"
     CDS             complement(195..773)
                     /locus_tag="ACGE4U_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8392440.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(793..1461)
                     /locus_tag="ACGE4U_000247"
     CDS             complement(793..1461)
                     /locus_tag="ACGE4U_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8392441.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1476..2546)
                     /locus_tag="ACGE4U_000248"
     CDS             complement(1476..2546)
                     /locus_tag="ACGE4U_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8392442.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2717..4081)
                     /locus_tag="ACGE4U_000249"
     CDS             complement(2717..4081)
                     /locus_tag="ACGE4U_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFF8392443.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(4078..5637)
                     /locus_tag="ACGE4U_000250"
     CDS             complement(4078..5637)
                     /locus_tag="ACGE4U_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8392444.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5693..8173)
                     /locus_tag="ACGE4U_000251"
     CDS             complement(5693..8173)
                     /locus_tag="ACGE4U_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392445.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(8157..8492)
                     /locus_tag="ACGE4U_000252"
     CDS             complement(8157..8492)
                     /locus_tag="ACGE4U_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392446.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8506..9540)
                     /locus_tag="ACGE4U_000253"
     CDS             complement(8506..9540)
                     /locus_tag="ACGE4U_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFF8392447.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9630..10310)
                     /locus_tag="ACGE4U_000254"
     CDS             complement(9630..10310)
                     /locus_tag="ACGE4U_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFF8392448.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(10594..11697)
                     /locus_tag="ACGE4U_000255"
     CDS             complement(10594..11697)
                     /locus_tag="ACGE4U_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFF8392449.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(12326..12796)
                     /locus_tag="ACGE4U_000256"
     CDS             complement(12326..12796)
                     /locus_tag="ACGE4U_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFF8392450.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            13126..14766
                     /locus_tag="ACGE4U_000257"
     CDS             13126..14766
                     /locus_tag="ACGE4U_000257"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFF8392451.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            14920..16242
                     /locus_tag="ACGE4U_000258"
     CDS             14920..16242
                     /locus_tag="ACGE4U_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFF8392452.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(16310..16744)
                     /locus_tag="ACGE4U_000259"
     CDS             complement(16310..16744)
                     /locus_tag="ACGE4U_000259"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8392453.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(16809..17645)
                     /locus_tag="ACGE4U_000260"
     CDS             complement(16809..17645)
                     /locus_tag="ACGE4U_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFF8392454.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(18387..19013)
                     /locus_tag="ACGE4U_000261"
     CDS             complement(18387..19013)
                     /locus_tag="ACGE4U_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392455.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(19308..19643)
                     /locus_tag="ACGE4U_000262"
     CDS             complement(19308..19643)
                     /locus_tag="ACGE4U_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFF8392456.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(19630..19914)
                     /locus_tag="ACGE4U_000263"
     CDS             complement(19630..19914)
                     /locus_tag="ACGE4U_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFF8392457.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(19970..21493)
                     /locus_tag="ACGE4U_000264"
     CDS             complement(19970..21493)
                     /locus_tag="ACGE4U_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFF8392458.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(21486..22661)
                     /locus_tag="ACGE4U_000265"
     CDS             complement(21486..22661)
                     /locus_tag="ACGE4U_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8392459.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(22665..22850)
                     /locus_tag="ACGE4U_000266"
     CDS             complement(22665..22850)
                     /locus_tag="ACGE4U_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392460.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(22816..24510)
                     /locus_tag="ACGE4U_000267"
     CDS             complement(22816..24510)
                     /locus_tag="ACGE4U_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFF8392461.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(24507..24980)
                     /locus_tag="ACGE4U_000268"
     CDS             complement(24507..24980)
                     /locus_tag="ACGE4U_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFF8392462.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(25049..25204)
                     /locus_tag="ACGE4U_000269"
     CDS             complement(25049..25204)
                     /locus_tag="ACGE4U_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFF8392463.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(25338..25718)
                     /locus_tag="ACGE4U_000270"
     CDS             complement(25338..25718)
                     /locus_tag="ACGE4U_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFF8392464.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(25722..25937)
                     /locus_tag="ACGE4U_000271"
     CDS             complement(25722..25937)
                     /locus_tag="ACGE4U_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392465.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(25940..26347)
                     /locus_tag="ACGE4U_000272"
     CDS             complement(25940..26347)
                     /locus_tag="ACGE4U_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFF8392466.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(26613..28088)
                     /locus_tag="ACGE4U_000273"
     CDS             complement(26613..28088)
                     /locus_tag="ACGE4U_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFF8392467.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(28078..28926)
                     /locus_tag="ACGE4U_000274"
     CDS             complement(28078..28926)
                     /locus_tag="ACGE4U_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFF8392468.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(28963..29325)
                     /locus_tag="ACGE4U_000275"
     CDS             complement(28963..29325)
                     /locus_tag="ACGE4U_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392469.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(29326..29484)
                     /locus_tag="ACGE4U_000276"
     CDS             complement(29326..29484)
                     /locus_tag="ACGE4U_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392470.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(29625..30249)
                     /locus_tag="ACGE4U_000277"
                     /pseudo
     CDS             complement(29625..30249)
                     /locus_tag="ACGE4U_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(30246..30557)
                     /locus_tag="ACGE4U_000278"
     CDS             complement(30246..30557)
                     /locus_tag="ACGE4U_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392471.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(30601..30894)
                     /locus_tag="ACGE4U_000279"
     CDS             complement(30601..30894)
                     /locus_tag="ACGE4U_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392472.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            31088..31735
                     /locus_tag="ACGE4U_000280"
     CDS             31088..31735
                     /locus_tag="ACGE4U_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8392473.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            31796..32941
                     /locus_tag="ACGE4U_000281"
     CDS             31796..32941
                     /locus_tag="ACGE4U_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8392474.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(33380..34198)
                     /locus_tag="ACGE4U_000282"
     CDS             complement(33380..34198)
                     /locus_tag="ACGE4U_000282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392475.1"
                     /translation="MVQGLFGGGTSYEGQSISDIKEDVNNWKIYTENVKSQIVEKKEA
                     LIKNGFWNKIPWNFQQTINESIFCLGTFLSDFQIVESAFEENFISEREIKLLRKIGKN
                     SVQFNHSYGKYFKEEYEWREYGNPEFEIVESIYQHGRDFFVTLQDASNAAGRLEDYMS
                     NNSIYNKVNIFGNSYNTNIQQGHNNNMIVSIDDMSELLSKLDLLLENLNDSLPEEEVE
                     EAQEYIETIKDEAIKEKPKKTMLRFAVDSLKKLKWSTEFLASVATIAQLVAPLL"
     gene            complement(34298..35623)
                     /locus_tag="ACGE4U_000283"
     CDS             complement(34298..35623)
                     /locus_tag="ACGE4U_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392476.1"
                     /translation="MENIEETFLKVLSPYISDSILRFQEQEKNYKFGFDIKNYNDSLH
                     VVFQYNKTGKIGIKEVHIFQIFLAKVNIEASKSKLNYTTLLDTNALFFSKKLLPYLHS
                     YEWALRKLIYLLAPAYFSDNWAEESIPNEIISSIKRKQIQAIGKFDSKNLLQWIDLYD
                     FEEYLFGENYILVTQENEENIVRYKEISQDQLLKLFQDNSHTISDSYSLWSEVFSKYM
                     DVKLEEIQTDMSLIREGRNIVSHNKEIEMALYQKLIKKLKRYTNQLEKAFQKILAGDI
                     AGEDLSDMSDDFEGYIGNYFSNIDLAGVTKLSESLSAMKVLNTDSMKKSMESIGQTLS
                     VQQEIMQNFDTEGIRRATEGINQSLQSIKILNTDSMKRSIESIGQTLSALSAQRAIMQ
                     NFDTKGIRSATEGINQSLQSMKIVNTDSMKRSMESIGKISSTNQVLKSE"
     gene            complement(35792..>36437)
                     /locus_tag="ACGE4U_000284"
     CDS             complement(35792..>36437)
                     /locus_tag="ACGE4U_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392477.1"
                     /translation="INLISSAKTLTESIEVCMKNFLAEKDFKSFKNKILSKPYDEHFS
                     YRFLLHMRNYSQHGHLPVNIHQQRVYFDLDEILAMPHFDFNGQLKKELDVLRKDIYNQ
                     FRDSPRISYVYTVAKFNLVITEIYFNYLKEIKPILIKLNDEKNELLLNTKFQLINSDG
                     SSSDMLFYEFDGENYHCFNRKDNSISMYADIKREVKKILKVEEQYYKEIEKRNQ"
BASE COUNT        10647 a         7373 c         5979 g        12438 t
ORIGIN      
        1 aattaatttc ttcgttatta aattaaaaca cttcctggaa caccgacata ataaataaaa
       61 atataccctg ataatcatga gagaataaca aatttgtatt tatgaaaaac tacctatata
      121 aaaaaaacaa gatataacga tcttcgtcat atcttgcttc ttcacctaac tatttttctc
      181 cctatataac agctttacgc ttctcctaat acaccattca aatataaatc gatcaattgc
      241 gccacattta gcggttcttc cacaggtctt ttcgaccgag tgatgtaagg tgctaacact
      301 aagaaaaaat gccttgctgc gatttctttt gaaatcccat ttcttagcac agattggttt
      361 gctgaaaaga tttcttcaaa aggttcacga taatttttct tccatagaaa gaaaagatga
      421 tctcttgttt cctttgaaag attgctttca agatcgtgaa gcatcagaaa caaatcgacc
      481 agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg ttacttgaaa tcccgtttcg
      541 cttgtttgaa cgatttctct caaagctttt ccaacatctt ttaagtggtt ttctaaaact
      601 gccgtatata gtttttcttt atcaccgaaa taatgataga gattgggttg ggtgatccca
      661 actttttgtg cgatctctct tgttgaagtg ccgagatatc ccttggacat aaacaagtca
      721 gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt atcgatggac catttttcca
      781 cctgcttttt ggttattctt tttctttcaa tacaccatct tccatcataa atattctatc
      841 gcatttgtca atcagccgtg tatcgtgagt aaccatgatc gttgctttat ttttgtcttt
      901 tgattctttt gccaagatat cgactacttc tcgtgccttt tctgaatcca aactagcagt
      961 tggttcatct gctagaataa tggacggatc attgtacaat gcacgagcga ttgcgaccct
     1021 ttgccgttct ccaccagata gttcttctgg atacttgttg atcaacttat ccacgccaag
     1081 ctgctcaaat aattcttttg cttctttttg acgatgttca tgtttgatct tatctactaa
     1141 taataactgc ttttttactg tcagaaaagg aaccagattg gaagcttgca aaataaagcc
     1201 aatttcttta aaacgtagtt taacgcgttc actttctttt tgattactga attcttgatc
     1261 attgataaat acagagccac tgctaggcgt ctgcaatccg cctgccaaag tcaaaaaagt
     1321 acttttacct gaaccagaag gaccgatgat tgcaacaaat tcccctttat cgatactcat
     1381 ggttgtttct ttcaatgctt taactacttg gtcgccatct tggaaacttt tttctacttc
     1441 tttaaattct attgctttca tgctcttcct ccttcttatc caattgcctt caacggatcg
     1501 atttttacga ttgtccgaac agaaaatagt gcgcctaaca cagctactac cagcatcaac
     1561 aagctaatga cgccaaaaaa cagccaattg ctttggaatg gaaccgcatc aggtagaaca
     1621 agggatgttc ccactgttcc taatagacca agaagaatac caacaaatga taaaatgaat
     1681 gtttgtgcca ctacagaacg cgcaataaat cctcctgtga tcccttgtgc tttcattaca
     1741 ccaaagatat taatcttttg catcgtcagt acataaatga aaatcccgat cacaatagcc
     1801 gcaataacga tcaaaaatcc gatcataaat ccaaaagtca atacttgcgc attatatcca
     1861 ggtaattcat tgatgaattt agaaattttg atctgttcta aatcttcagg taaattactg
     1921 attttacctc tgacaactat tgcgttaatc cgtgcatttt cacttgtatc ttctttttcg
     1981 aagcggatct cttggtaagc attgatggtt gtatagagaa ctggtgagac attgaatttg
     2041 gcatgatccg taaagccagt tagtttaaac gtcttgtcac taccagataa cttaactgta
     2101 tctcctacgg ctagaccata ttcttctttc aaactaatat cgcctactgc ctcatcatcg
     2161 ttatcaaaag cttttccttc aacaagattg ggcatgatga attgattttt atcgataccg
     2221 aagaagctaa cattgatttt tccttcttct gtactgtctt tactagtcac gactccaggt
     2281 gtctgggcaa gatacgccac tttcccacct tcgacttctt ctgctatctt cttagtaatc
     2341 atggacatcc ctagatttga atttgcatct ttcgaaagca caatcgaatc tgcctcccac
     2401 ttgtcgactg ctgttcgatt atcttgagct aagccataag ctaacccagt caaaaaaaat
     2461 accaggtagg cgatcaaaaa catcacccct gcaactaacg cataacgtaa ctttgaatgc
     2521 ataatttcat ttaatgctaa aaacatttaa ctcctcctcc tgaaataatc attttataca
     2581 acaattttat cacttgataa atttttggcg aatattatgc tcaaaaaatt cttccgctat
     2641 ttcattatat gaatttttta ttctcgattg attaatattt attatatccc cacacaataa
     2701 aagaccgtga tatttctcac agtcttttat ctactctatt gctatttgct ctcttagcta
     2761 gtactttaaa agctaactca ttgaggtagt gaaaacgaga ggtgtgttca gacagaaatc
     2821 caaaatcctc aactgctagc tgtggaatcg tagttttgtg tttcacgata tccgcaagca
     2881 ggttcaaatc tgataataag aattcttggc ttatcagctg tccgccaatt atttctaatg
     2941 tatccggatg gtagatcagc tttgcattta ctgttttgtt ttcttcatcg tattgtggaa
     3001 atagatcaaa ttctcctgaa cacgaaccgg tattttgttc caagaatggc gcttcatctt
     3061 ctgtcagccc tacagttcct aaaaagtagt ctataatgac tgctgctgtt atcttttgtc
     3121 tgaggtcgta agcggttggc tgaccactca aagttagcgc tactgctctt gccatatgta
     3181 cagctcgagt aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa ctgactggga
     3241 cgatatctcc aatcgcatag atgtccggat cacttgtctg cagatattca ttgactatca
     3301 cagtctcatc ctcatacaaa gccacttttt cttttaacaa tgtcgtatta ggacgagttt
     3361 gggcagaaaa aatgaccata tctgccttta ttgcttgaga tagaagttgt acggatacta
     3421 ctttctcttc tgattttttc caatcgatca agaattcatt tggataaaaa ttgatttgct
     3481 tagtttgcag ctgattttga aaactgttga tcatatcctt atcgaaatat cttgataaaa
     3541 cattcggcat ataatcaact agatgaataa tgtatccttt atttctcaaa gcatctgcta
     3601 attccaatcc aatataacca gcaccgaaga tcactacttc ttttgcttct tttaaagcgg
     3661 caagtacttg cttagattgc actagatttt tataggtgta gacattttca gcttcaattt
     3721 gtttatacaa ggtgacagga gaggaacctg ttgctacgat taatttatca taggttatgg
     3781 tctcgttttt tccttctttg gatgcgagga tgagctgatg tttgtctgga tcaagttcag
     3841 ttactctcca ttcggtgatc agttttgccc cactttcttg aagagtccgc atgttatggg
     3901 caacttcagc taaactacgt atctttcctt ttaaataaag gttgattcca tttgaaacat
     3961 agcctagtcc atctttatga tcttcatcga tcagtaaaac ttctgctttc ggttgaagtt
     4021 ttcttagatt tagggcagct gtgatgcctg catgggaagc accaatgatc acgattttca
     4081 tgttttattc atccttaaat tcaatttttc taaataacat tcttttaaaa attggttcaa
     4141 ataggggaaa tcataattat attcgttgct agtgaataca aaaactttag cggacttctg
     4201 tgacgggtac tctttcaatt ggctagtcaa aatgatatca tactgctttt ctttctcgta
     4261 ttcgactaaa tcatatttga tcgtattcag caattgttcc tggatgattt ctttcacgat
     4321 atccgccatt agcctttcat aaggaagatc ataagcaatt tgcagacgta attctaactg
     4381 cttgatcagc gtccgcaaaa tagatgcata gcgagcaaat aattcaaagc gggcattttc
     4441 ctcgtctact tcagtaaaat caacaatagc aaatgtttgt ctgaccaatc tttcacataa
     4501 aaccaggaat tgtttatctt ctacttcagt taaccgctgt tttaatcctt cttggccaaa
     4561 aatattcatc catcctctaa aataggcaaa atgagcgtga ttttgcatca ctgtcaacgc
     4621 aatcttttgc gaaatctctt ctggaaaaac ataaaaagag aaatattctt ctatcgattg
     4681 tttaaacgtt cgattcaatt gagctactac gtcgtctttg tatttttcgc ttatcaaaaa
     4741 ctcctggaca tgcgagttat gtgtatctaa tgtaaagtta ctgatcatga aaagataaaa
     4801 aaccaacgtt tcattttgat tccaaggaaa ggcatattga aagaagtaac gggtcaaaaa
     4861 tgtatggagg tccaataaaa attgtgtcgt ttgaaacaat ccaacaggaa gattgctata
     4921 atctggtttg gattttttcg tacagcgcat tcgcttcttc ataatggaaa gccatatttc
     4981 aaatttcaat cgtccttctt ctgtaatagt aaaactaaac tctttttgca gcagatctaa
     5041 aaaagtgttt cctgataaat cactgtattc ttttttgttt tcaagataag gacgtccaaa
     5101 ccaaaataat tggaaataaa aataacaaat ttgactttct ggaccggtca gccgcccgct
     5161 ttttattttc agatgaaact catttaaaga atcatttaat tccttcactc tgcgagacaa
     5221 agtagccgaa ctgatattaa gttccatgac aagtttctgc cgatcaaatg agccattttc
     5281 caacagataa agaagtatct gatattttgt agaatagcgc aagtagcaat gataataaac
     5341 atcgactgct gctatatttc catgtctgat taataaaata ttcccttctt ggataacaat
     5401 ttccaattgt ttgatatttt ccaagttagc ttgtaaataa aacagatctt ctttcaaggt
     5461 agcccgatca actgataagc gggtaagtac gttattaacg gaaatctctc ctttttcctc
     5521 taataaaagt tggaaaagtt tgatttgccg ttgttcattt ttttcaagca aactgaaaag
     5581 ttccaagtcc actcctccaa gcatttctga ttttaacagc agaagtttcc ttctcatcct
     5641 tgaaaaagag gatctgtgac agttatcaca gatccataca aattctaatc acttagctgg
     5701 ttggcccagc tgtcagttcc catgtgacca tgccttgata cgttgcgcca ggttgagcat
     5761 tcgtactgct atttacctct aagagtaaac catcggtatc gggaaagtca acgagaacct
     5821 ctcctttagc tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc tgaatcaatt
     5881 gttttgatcc agaatgattg aagtataatt ttgttccgct taattcttga ccttgatcat
     5941 tcttaaataa agaagtcaat gtccctgtca gttgccaagg agacgtgctt ggaagccgag
     6001 tatcccgcac atcaaaagaa aaatcgcctt ttgcttcata ataacttgtc ttcaacggtt
     6061 tggggagatc gttaccaaat gtaaaatcat ctggtacatt aactaatgtt aattcttgtt
     6121 tggggacaag aattgtttta gcagatgttt ggacaactgg gttaggaagc ttagcaccat
     6181 ctgctaatgt aatattgctg gaaagtacag ctgcctttgt cagtatgctg acggattgta
     6241 cagaagtatc tacagtattt aattgatagt tgtaagtaag atgaatggta tctcctattt
     6301 tgctcaatag atctgctggg acagaaactc cttgttgaat gccggtatct gcgatagttg
     6361 taccatttaa ttttaaggaa ccattgatat aagtagtatt ttcaggaagt gagatcgtga
     6421 tttttttgtc ggttatcgca tccccagatt ttgttaacgt gaatgtcaca ttttcttgaa
     6481 tcgtatctcc tgatagagca gacaggggat ctcctgtctc aggttgtctg ctaaggtttt
     6541 ttagctgact atctgcactc acagatggaa aattagccac tgttatttga ggaagagtgg
     6601 tgattccagc ttgttcagac tgccatttgt acgcaaaaga cggatacatg ttagtaagta
     6661 catcgtccac caagcgataa tcaaaataat attgccctga agccaatgca ggtagtgtag
     6721 aagtagctgt atttacaccc ccgttatttg attggatcgt ttggaaaagt tgatctgtcc
     6781 atcctgtcgc taatttttgg gttcctttcg aataaagcct gtaatggatc gaaccctttt
     6841 ttagttcagt tgctgaccaa ttggccgtga cttggtacga tgcagaagaa aggagctttc
     6901 cccactgact atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc ttgtactgct
     6961 ccaatacctt taaagcaaca ggagaaagca atccgccttg cgcatctttc ccactgtaca
     7021 cgatattggc tgtctgattc aatccacttg tgcttgctgg agtatgagtc agtattgctc
     7081 cttgtgtaac gttttttcct gtaaaatagt tgactacgcg atcaaagcga ctaaatggca
     7141 actgatcaaa acgtccttca gtcagcccca tcgtcaatgt ccgactattg gtagagcctg
     7201 gaaggttctg gttatttcca tccacgtatt ctgtggtatc agcaaatgta ttatattttc
     7261 ctgttgaatc acttgttgag atgtaactat tccacgtatt tatctctgca cttgcagaaa
     7321 atgcatatgc tcctggattc aagtttccgc cagttaattt aggataaaat ctacctgcag
     7381 ctggagctaa aatcgaattg aacatacgat cttggataat attattcgaa tcataaaaat
     7441 caacagaaaa aacggccttt ttagacatag aaggttgtaa tttacccttg gtcaaaattt
     7501 tgtttggact cgtatcatat ttatctccgt tttctgtcat cttcgcgaca ttagctgtaa
     7561 catcaaattg aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata atagcaataa
     7621 tatctgccaa tagagtattt gtatcccacg accccatccc aaaacgcaag gtatggcttc
     7681 ctgctgataa taaactaccg ctagtaacct tgatgggggc accggcaata tacaagtagg
     7741 cgttgttcca gtcaattgct tttaatacat cactgcttga tacatgttcc ggcaaattga
     7801 tgatcgccac aaatgcaaat ccttgtgcag tgatattcac caaatcaagt acatttgtta
     7861 gtttcacatg gctttcccaa tgaaaacgga ccgtgccacc tttccaagga tcatttgaac
     7921 catcgttcaa ataagtagca gtcaatttag gcaattggtt agccccagtt agttggacaa
     7981 gcttcccgcc attgactgca ctacgtccac catttggagg tacgatattt tcatacgtgt
     8041 atggatcata agaaatggca ggaggcgttg ctccaacagc ttctgccgaa ggtgaaaaga
     8101 taaatatact taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat gaaaatttat
     8161 tttgtagcaa catgttcatc ctccttttcc tttcttctcc ataacaacaa ccacgcaaga
     8221 agccataatt ctataccaat gatcaaataa agaaaacgga tcttttcttc attcgtctcc
     8281 gggaatacgc catgttgatt agattcttta taaatcggta gctgtgtctg ctgttttgat
     8341 gcgccattga ctgaaccact tggatgcgga actggactta gatcataaaa tgtaatcgta
     8401 cctgaacttt tggtatctgc ttggactggc tcttgccagc tgaagaacag caaacttgca
     8461 ataatccctc ccaataagat cgatgttttc aacgaaatca cctcatcatg ttgtttctcc
     8521 ttttttcttt ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca ctagaactgc
     8581 agcaaccaat cctgcataaa gccatgggaa agattgttct tgtactgcaa aaacggaatt
     8641 ttcatttact ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa atcgccattc
     8701 attactgtcg cctgattttg ctatgatttc tgcgcggtaa tcgccggctt ttgctttatc
     8761 accagctaaa tcaagattca agtgaaaatt cgaatttggt gcaactaaaa attcattaga
     8821 cgtattttca atataaagct gattttcata aaagatcttg ctcgtaaata tcaaattgct
     8881 gataatccta ggctgaggat tttggaaacg aagctgaaca gttggctttg cgttgacctc
     8941 gtccaaatct cctgataaaa gtttcagatc cggttctatc gtctcttgac tttcttgtaa
     9001 aacgactgcg agattataag caatacgatt tttgatttgt gctccttctg tagcattttc
     9061 tgtttcagct tcttctttag gtgaaacatg aatacccgct aatacacgtc ccttaaagct
     9121 ttttgctggg attttcactt ctataggtat agttgtttct gcatgtgctg atacattgat
     9181 ttcctttttc gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct catctaacgt
     9241 tgggtttttt tgactataat cgatcgttcc accatcactc gttaccgccg gattgacaga
     9301 gacctcaaat gttcgttctt catctgaaga attgctgata attacttcta gtgtctgatt
     9361 ttgttccggt gccactaata aatcaaaata accgattgct ttatctattt ggttctctgg
     9421 aaataccggt ttgattccaa agtctcctgc tgaatccttt gcttggctga ttgacggaaa
     9481 aatgaaaaat agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac tttgattcat
     9541 tgtccaccac atcctttcaa caacaactgc ggtaaaaaat ccttcaccta tagtattttc
     9601 accgcagttt gttgaattta tttcatactc tatgctactg ggccagccac gaggttccaa
     9661 gtcaaatcag ccgtgaatgt tccagctgct gctttcggca ctttcagtgt gatatttgct
     9721 ggatcataaa cagaaagcca tgttcccaga ccttgatctt ttgctgctgt aaatattggt
     9781 gtcgctgcac ttgctttact caacccagtt acagtttgac tagttggtcc agtggattcg
     9841 ttatctgacg tacttttaac agtggatgtt ccaaaatcta attcagcgcc ttgcaaaact
     9901 tgtgaaccgt ttacaaaatc tgaaaccgaa actgtcacgt tccacccttg tgcttggcca
     9961 tctgcaccta cacctcgacg atctgatact tggagataag gtgtatcgtt ttttgaagcg
    10021 tctacttgat acgtttttgt tccgctttca atctcgtgtg taccaaatgg caattcagga
    10081 actacatcca atgttaaaga acctgtctgg ccagttgctg gattttccgg atctgctggc
    10141 tctactggtt ttgaaggatc agtagggtta agaggctcaa cggtatttgt tggttcagta
    10201 aaggtaatat ctccttttga agtcgcttga ccctcagcaa aagctggtgt tgccaagccg
    10261 cctagaagaa gtgttgaaag taataatgtc ccaatcatgt tctttttcat aataaatcac
    10321 tcctttttct tagtgaccta agtatagtag agacagtatg caaaaaatcc tcatgaattc
    10381 atccgcttac ttgaaattat gacccttttt gtataaaaga gaaaaaaaca ataaaatcgt
    10441 ttacatgaat attcttcctt ttttattaag tatgaaaaaa tatttcaatt ttattttttc
    10501 gtttcaaatt attctattag tgacatcctt gaactataaa aataggagta cggcatcatc
    10561 tgccgcactc ctattttttt cgtcatttat ttcttaaata aaacgattct cacgcaattt
    10621 tatttttttg atatcttcta ctgtttgtgt tccagctaat tgcatgacca tttccaattc
    10681 tgttttaaag aaatcaaaca cttgatggac tcctgtactt cctcctaaag acaatccata
    10741 aataacgggt ctgccaatag caacaagatc ggcacctgat gcgattgctt tgaaaacatg
    10801 ttgtccacgt cgtacaccgc tgtcaaacac gattggtaca cgtttatcta ctgcttctgc
    10861 cacatattgc aacgaatcaa acgcagcagg ccccccatct aattgacggc caccatggtt
    10921 agatacccaa attccaccag caccagattc taatgatcga tagacatctt cttctgattg
    10981 aacacctttc acgtagactg gcaaatcaga atgagcagcg ataaattcta catcttttgg
    11041 actaagtttt tgtttggaag atttatatac agcatccatc gtttggccta ctcctgattg
    11101 ataagcctga acgatcggca ttggcagcgg gaaggtaaac ccgtttctgc ggtctgtttc
    11161 acggtttccg ccaactgttg catccgctgt caatacgatt gcttttgctc cattacgctt
    11221 ggccacttca agaatatcca aattgattcc attatctttg ctcatataaa attgaaacca
    11281 ttgcggtgct ttctccccgc ctgcttctct tatctcttct aaagtacaag aagcgtaaga
    11341 acttgctgta tagatcgtcc caaaatctgc tacacctttt gcagaagctt tctccgcctt
    11401 cacatgagct aatccatgag cagcaactgg tgccatgatg attggtgcag tcagcatttc
    11461 ttcgtcaaaa tgtgtagttg tatcgggtaa ttctacatct cttaatacat gaggaataat
    11521 caaacgatga ttgaatgccc gttcattttc ttgataagta aataagtcgc cagcaccact
    11581 gctgatgtaa ccataaccac cagctggaat cacttgtgaa gccgcgattt ctaaatcttc
    11641 catattgata aaatctacta ttccttcatt tgtacctgct tgatatgttt tttccatctc
    11701 aatgccttct ctctataaaa tttgaatgat gaacaatatg atagcgttta cactttttat
    11761 attactcttc gacattgata caggcaaata atctgtttac atttcagcca ttccagatgt
    11821 cactactctg gaagaaatga ttgtaataaa aatagataaa aaaccgatgg gactttgaca
    11881 aaagtagtgt caaagtccct ttttttgaat aaacggtgtt caaaagccat tttctcggta
    11941 ttaagtcaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt cttactactc
    12001 gaaactaagc gactttttca caacctctcg ataaaacggt gttcaatcag ctgaccctcg
    12061 gtattagctc aaaaaatggc aaaatatgag gaactatttt gccatttgtc tctctaatac
    12121 tcaggtcaga acgttgactt cacagtctct tccttaaacg gtgttcaaaa acttgcatct
    12181 gcggtaataa gcccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta
    12241 ttacttgatg cagggcaggt ttttcacgac ctctgattca cggtgtttct ttttcatcaa
    12301 catttttacg aaatactgtg gaagactatt gaaataagtg tggattgttt ctttgtgttt
    12361 caatcgtttc ttgccattct tcatttaatt ttggattacc gttttcttcc ttgaactcat
    12421 aaggtcctaa ttcaaaaatc ccagacagta tttgattcac gatttcatat agttcagtgt
    12481 gatcagacac atgattcttg aagatcatct cattatcacc acgtttgaac ctcacattcg
    12541 ggctctcatg atgaacctct atgcattttt ctcctttttt taactgacgg acttctgaaa
    12601 acatctcata aatatcttct ccagtaaaac cttcagcaag cgccagactg tccacaagac
    12661 cgcctactgt cagttcagag attattgctt gattggaatc taattttaat gtatagcttg
    12721 tcgcttctac tacgccacat gcattgcaac cgccttctga tagttcgctt tttctaatca
    12781 attgaacttc tttcattttc tcttcctcct cttaattttc tacttatgta tttttatcac
    12841 ctgacatagg tgactcctat caacgctatc atgtgctttt ttcctcagaa atcctattta
    12901 tccattccat ctatccctta ttcttctact tttctttctc tagcatataa gagatacgca
    12961 tagtattcaa gcaaggattg tcgatataaa aagaacaaat actgctattt tgatttcttt
    13021 tggctatatt acatataaat aaaatttatc atacctgctc tttttgacta tatcatcatc
    13081 tgacttctat acttcgtttg tattaaaact taggaggaat cattcatgct tacagggtta
    13141 aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga agaacgggca
    13201 aaatatggaa tcactggtat gcttccaagt actgtccaaa cactggaaca acaatctgtt
    13261 caagcttatg gtcaatactt aagcaaacaa acagatttgg aaaaacgtat cttcttaatg
    13321 aatctattca atacgaatcg gacattgttt tataaattga tgggcgaaca tttagttgaa
    13381 ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata caacgaaatt
    13441 ttcttgaaac cgcaagatgc tgcatttctt tcaatcgacg aaccagaaaa catcaaagca
    13501 agtttgaaga acgcggcaga tggacgagac attcgtttga ttgttgtgac tgatgcagaa
    13561 ggaatcttag gtatgggcga ctggggtgtg aacggtgttg acatcgctat cggcaaattg
    13621 atggtctata ctgctgctgc tggtattaat ccagcacaag ttttgcctgt atcgatcgac
    13681 gctggtacta ataatgaaga attattgaat aatcccttat acttaggcaa tcgtcatgct
    13741 cgtgtagaag gcgaaactta ctatgaattc atcgatcaat tcgttcaatc agctaccgaa
    13801 ttattcccag aattgttatt gcattgggaa gattttggac gaggaaatgc tgcagcgatc
    13861 ttagaaaaat acgaagatca aatcactact ttcaacgacg atatccaagg tacaggaatt
    13921 gttgtcctag caggtgtatt aggcggattg aatatatccg gtgaacaatt gaaagatcaa
    13981 ataattttga catttggtgc tggtactgct ggtgtgggta tcgcaaacat ccttttagat
    14041 gagatgatcc gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta tcaagtagat
    14101 aaacaaggtt tattgtttga agatacagaa ggattgactc ctggtcaaat cccattcgct
    14161 cgcaaacgct cagatttcac taatgctgat gaattgacaa acttagaagc agttgtaaaa
    14221 gcgatccatc cgacaatcat gattggtact tctacccaac ctggagcatt cactgaagaa
    14281 atcgtgaaag aaatggctgc tcatacacca cgtccgatca tcatgccttt gtctaatcca
    14341 acaaaattgg cagaagcaaa agcaaaagat ttaatagaat ggacggacgg aaaagcatta
    14401 gttggaacag gtatccctgc tgctgatgtt gaatataatg gtgtaactta ccaaatcggt
    14461 caagcaaata atgctttgat gtacccaggt ttaggacttg gtttgattgc ttcaacagct
    14521 acacgagtaa atgctgaaat catttctcaa gctagtcgag cattaggcgg aatcgttgat
    14581 gtaacaaaac caggagcagc tatcttgcct ccagtagcaa aaatcactga attttctcaa
    14641 acgattgctg aaactgtcgc aaaatcagtt gtcgcacaaa acttgaatcg tgaagaaatc
    14701 acggatatca aagaagcagt tgaatctgct aaatgggttc ctgaatataa atctttagaa
    14761 gattaagatc gttatagtaa aagaacatac agaaactttg tatgacgtct aaaaagaaaa
    14821 aagcgactgc ggcaaaacag cggccagcca actcctgctc ttttgtcgct tttctttggc
    14881 ttttttcata tgatacagaa taagaaagga tctgtaaaaa tggcacaaaa aacaacaact
    14941 gcaactgcag ttgaggaaaa gaaaaaagaa ggtttttggt ccacaaaaat caccggcgta
    15001 agtttgccac tttatttgat catggtcctt gtcctgatca ttgcaatggc tttagggaaa
    15061 ttaccgacga atatgattgg accaatcgca gttttggtta ttttcggtaa tttatttcat
    15121 tttatcggga caaaaatccc tatcgtcaaa agttatttgg gtggtggttc cgtttttgcg
    15181 atcttcgctt ctgctgcgat tgctacattt gggatcttac ctgaatatgt agtgaaatcc
    15241 acagaaaact ttgtcagcaa tatgggcttt tgggactttt atattgctgc cttgattact
    15301 ggtagcatcc ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg ttttattccc
    15361 gttgcactta tttcaatggt cgtctccttt tttgccgtag gactcgtggg catgcttatc
    15421 ggaaatggtt ttgcaaattc cgttttatac atctcattac ctgcgatggc tggtggtgtt
    15481 ggagccggag ccgttccttt atcaacgatt tatgcaaatg ttttaggtac ggatgcttct
    15541 tctatcattt cccgcctgat tcctgcgaca gcaatgacaa atgtcttagc tatcatcggt
    15601 gcagcattag ttgctcgtgc tggacaatca atgccaaaat tcaacggcaa tggtaaatta
    15661 atggaaaaag gcgatcttgg cgatggaaaa cctcgaactg tgaaaccttc cattccacag
    15721 ttaggtgttg gtttgatggt atcattgact ttcttcatct taggacaaat tggtaactat
    15781 tttgttccta aagtccatgc ttacgcattt atgattatta tcgttgtcat ctgtaaaatc
    15841 gccaacctat taccagagta ttatgaagat gcagctatca tgttcaataa cttgattgtc
    15901 aaaaacttga cagcagctgt tcttgccggt atcggtattg cgttgctgaa tctgaatgta
    15961 ttagcttctg ctttgacatg gcaatttgtt gtcttatgtc tgacaagtgt tatcgcgatt
    16021 tcagttgttt caggatttgt cggtcgtttg tttggtttat atccaattga atcaactatt
    16081 actgctggct tatgcaacaa ctcgatgggc ggaacaggaa atgtagccgt cttatctgca
    16141 gctaatcgga tggaacttat tgcttttgct caaatgggca atcgacttgg tggtgccatc
    16201 gttttgatca tctctggttt cttgatgcaa ttactttctt agtcaaaaaa acaaaaatac
    16261 ctgatcatca agtatcggct tggtgatcgg gtattttttt gataaagatt tacaggattt
    16321 tcaaaaatct tttttgcatt ttatcggaat gataaccgat gtgttcataa aattggtgtg
    16381 cttctactct gtattctgca gaattaagcc ttattgtcgt tattcctcgt tttttcgctt
    16441 cttcttctac gccagtcatc aaagcctttc cgatgcctct tttttcagca tatttagaca
    16501 cagcaagtgc cataatattg aacattggat cgctaaaaat cgattcatag gactccgctt
    16561 gtacatagcc agctgctttt ttagaagtag gatcttcata aaccaagaaa aaatttttct
    16621 ctggctcctt cagcagtttc ttcatttgtc tcaccgtaac ggttaatggt atatcatacc
    16681 ctaattgatc cgcattgatt tcacgtagtg cttctgcatc ttctaaacgc atttcccgta
    16741 acatccaaat caccagccaa tttctgattt tttaaaatga atttttttat catagtatac
    16801 tctttttttc atttagaaca atatgtttca gtgattttga cttgcgttag tctagattct
    16861 cctaatgttt gtaagatgac aaaggcattg taacaattga ccaaaagaac cacgaggaca
    16921 attacttgaa aaataaaggt cacttttttt ggagacaaaa ccctgctaaa tcttgctccc
    16981 cacaaacctc caataacagc agcggggata acataaaaca acatagataa atcaaagcgc
    17041 ataaagctac ttgttaaagc aatcgttacc aattttgcca gttgagaaaa gaaaatcgta
    17101 gatagagaat atagtgttgc ttcttttatt ggcaatgcaa acatcagcat caacaaagaa
    17161 acattgactg gtccaccacc aatacctaaa aaactagcta aaaagcccag aaccccccca
    17221 catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag
    17281 aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg cacatgcttc
    17341 tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac tcctccaaca
    17401 acagctccac tagataccca accgacaatc tgccaattca gtgatttccc gcttgatact
    17461 tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc
    17521 cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac gcctccaccc
    17581 atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata aatcagacca
    17641 atcatcttgt atttcctttc tcttgtttat atcttttagc gattgttact cttttctttt
    17701 attatacagt aaaagaaaca aacgaggctt cgaaagcttt tctacttttt aaggaaattt
    17761 agtgctaaac gaaaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca
    17821 aacaaaaata gctcctcata attttcgttt ggttgaactt attaccgcag atgcaagctt
    17881 ttgaacaccg tttaagaaag aggtcgtggg cctgccgttc agctccgagt cacaaggaac
    17941 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttgg
    18001 caggagaaca ccgtttaagg aagagactgt gaagtcaacg ttttgacctg attattagag
    18061 aaacaaatgg caaaatagtt cttcatattt cgccgttttt cgagcgaatg ccgaggggca
    18121 gctgattgaa catcgtttat ttggaaaaag ggattgtgac aagacgtttg tcacaatccc
    18181 tttttttata tcttattaat tcattctttt gttctattct tcttcttccg ccatgaatga
    18241 gaaacccata tcaaagaact ataaaggtta tcaaaacagc gtttatgact aaatgaatta
    18301 gagttatcta tcactatttt tcaagcgatt taaaccaaat ggcatacaat atggcataca
    18361 aaaacttcaa taagccgtgg gggtttttaa atttcttctt gttctttaaa atcaatgagt
    18421 ggagctacat aatttgaatc cactttttca aacgtcactt tttgcatcga cgaatgtttt
    18481 ctttcccaag ataacgtaaa tagatttgac tctggactta taaagtctac ttcaccttct
    18541 agtattactt tcttatgcaa atattcctct aataaatata gacttgctaa agcatacaac
    18601 acattttcca aatttgcttc cttataattt gaagaacgac catgttttac tctgttatat
    18661 attttccacc aatctggtgt atcttcaggc cacccatctc caaaaggttt tagtgaagtc
    18721 aatgttaaag aattcataac cctaacacca ttatccaaac acattaattc gctagattta
    18781 ataacagctt taaaatcagt tatgctttta ttgctactgc tatcaaatcc gcaaagttct
    18841 ttgaaaataa tctcacactc cgaaccaatc gctagtagta gtttttgata ttcaatagag
    18901 aatgttttga aattattctc atcgagcatc acaaaatctg tggtattaat aaactcattt
    18961 tctaaatgta gataatactt ccaataaaaa tttgtaaact ctgctttgtc cattaaatca
    19021 cctcttttga attataacaa aaaaaacacg tccacaacag acgtgttctt tgatttctac
    19081 acaatcagct tgccaaaacc atccaatcag tcctagcatg cctgtattaa aattgaacgt
    19141 tcatcatcta tcatctttct tagtccagca tgtttgcaac cacacctaca attttcagtc
    19201 caacaataga tttcgactag ctaagtcttg caaaattcag gcagctaacc cttcatttac
    19261 taccttaatt atactaaata cccacaacgt ttacaagtgt aatcattcta agaaatctgt
    19321 ttagctatga tcgttgtata atctttaaac gccgttcctt cattgattga aataatttta
    19381 taactcttgt ttttccaagc tactttcata tcgtttgtta gttcataatc ttgatcgtat
    19441 cgaataataa acgtcaacgt tccttcaaga atggtaccta cactagcaag cacgtctttt
    19501 agcatttgtg aacgcacatt agcccaacat tcaaatacag tttcttcact atccaccatc
    19561 tgcccatctt catcttttac ccgttttgtc tttttaaact tgactctctc agtcaattca
    19621 tttacattct taatgattgg cataataagc acctctcatt tgttgtacta atgcttgtac
    19681 cgttactggt atccgttcgc tgctggcttc ttgacggttc aaataccaat gttgagtaag
    19741 taatgacacc gccaaatcaa attgctgata attatccata tctttatccg ttgcgctact
    19801 atcaatagca ctttttatat attcgcctgc tgtatcaatc aattgttgaa taaagtcatc
    19861 atcaatagtg tgatcgatcc gcatactttt ttttatgcta tccaaaatta ccattttatc
    19921 tctcctaatg aaaaaaaggc agtgggataa accgccgccc aatatttttc tatacttttg
    19981 gtgttacaac tggcgtaaac tcaatataca ctgaagcatt ttgatcaatt ttcttataat
    20041 cgttgcgaac gatcactgca agtccttgtg agtagtaatc gaatttttcc cattgagtcg
    20101 ttacttgatt tctacgagca acaaagatag attgtgcaat atcccccata atcataggga
    20161 atgttccagc tttaggattg gcaaacaatg tatctgcaat caatactact ggcataccga
    20221 ataatgattt accactaggt gctgtcacat cgggttgtaa aatgtaacgt ccatctgaat
    20281 ctttcaatgt atctaggtgg taatatccgc tttggtttag aattaccatt ttatttaatg
    20341 ctgggtctaa tgccacattg tatagttgtt tcaaatcgtc caacgtagca gccgttttct
    20401 tagtgaatgt ttttaataaa tccatgatat gcttattgtc agtgttttct accaatttcg
    20461 ctaactgatc tttgacctct tgtacaatat tcactgctga atcttccaca acctcatttg
    20521 ataaggcaat cttaccagca cgagtttcta ctttataatc aactgaagta aacatttcag
    20581 cgtcaatatc accaatttca gcaagttcag cttttgttgc taacactgct tgttggttag
    20641 ttgctactgg atatttacct tgtccatttg atactgtttt tactgttgca tattgagcta
    20701 agttataatt tgaacgtttc aaatcaaaga cttcaccgat tacttcttcg ggaacaactg
    20761 ccgctgcatt tacagtagtg actccatcac gcacttcgcc ttgtgaacgg atatattctt
    20821 catacccacg caattctgtt ttttcgttat cgattaaagt tttttccatg ttattactct
    20881 ccttttgtgc tttagaagtg gattctaagc tattgtttgt ttgttttgct tggttgttac
    20941 tcataaacga ttcgtaagaa cgcttattta cttggacatt tgatgaatca taagcgggaa
    21001 cggtcacgac actaatttca ttaagtgctt tgattttatc tattgatcgt tcaattgtgc
    21061 catcttcttt tttgtcgaat gaatctaatc caagcacgaa cccaaaggac atagaatcaa
    21121 caaccccttt tgagatattc tcatacacat catttgaata ggtcgtgtcg gttagctctg
    21181 cttcaaaatg taatcctgta tcatcgacat tcaatttcaa cgtacctgct ttgacactag
    21241 ctagtggctt tgaataatcg tgtccatata ataagaacac atttgataaa tccacctctt
    21301 ttaatgcttc gggtgtaatc acttctacaa agccgcctaa atcttttgac ggttgcccga
    21361 atttcaacgc atagcctgaa atagttttgc cgtctgttgg ttgtttttca ttttctttct
    21421 ctactttggg agaatcggct gttagctcag cttcttctgt cagccgtttt tccttttctt
    21481 cttcattatt caactggtgt aactccttcc tgttctttta attgatattt ctccaacgta
    21541 tctaaatacg taaagttcaa acttgctagt aaacgatcgc caccatccat ggggggtagc
    21601 cccattttcg accgcccctc attaatcgtg agaagtgacc ctttgacctg atccaataca
    21661 ttttttactt ttgtttcagg atcagtctct agtaactgat cggaattaaa gcggatattg
    21721 gtttcaagtt tcgtatctaa ttcactcaca aacacattaa agtaatgaat cagcgtactt
    21781 tggatatact ggagattact ttgaacggtg ctagaatgtt cattttcaac gcctaaacgc
    21841 tcaatcggta caccaaacgc cttggcaatt tgttttgtgt tccaatcatt ggaattgata
    21901 agcttcaaaa catcggtgtt tatttctaat gttttgtagt ccattgtttc atcaagaatg
    21961 atcgttctaa gagcattatc tccactactt gaaccgttgg cttcttcaaa cttttcacga
    22021 attgcgttct ttgctgatcc gtctaaatct gacttatgaa ccttcaaaat acctgaccca
    22081 ctgacaccac gagtaaagaa attgtgcaat gtgcggttgc cagcttgttg aattttcagt
    22141 tcatcacgta acgcataaag tggtggtaat cctgttaaac catcttgggt gaaatatttg
    22201 aagtgcaaaa tatcgctaga cttcacacgt ctttttttat caccaatctg ataagacaac
    22261 gtaccattat ctaattgagt aaccgttaca cttgagttag gtaataagtg aatttcttct
    22321 accttttcac cgttgcgttt aatctctgca aagctattac cgtttagcaa catattgacc
    22381 gccaaagcaa actttaaatg ccaaccatcc atttctgaat taggtttctc gtttagtaac
    22441 ttcaccagtt cattatcagc ttgtggcata ttgtttttga ccaattgaat cggacttgaa
    22501 gcaatatcac tagcaataat tcgaacggct gcaaacacat cactatttct taatgcacga
    22561 acgcttgtaa agctggtggt gtaatcatcc gattggattg ataccacatg ttgtaggaaa
    22621 ggatctcccg ttgtattttg tctcgcttta aaaaatgcca tcttttaacc tcccttctgt
    22681 tctgtattta gcatgaccgc taccccaata agcactatgc ccagcgctaa caagccatag
    22741 tagacatttg ttagtaaggt gatagcaacc aacacacaca ccaatccaag aagtaaaaga
    22801 acggtatgaa tgtaattaga aagaaaattc ttcacttgta taaaattcat tgtctgcttt
    22861 cgctccttcc gttgtggtga agtaatccat tgcaaacaca taggcattga ttaatgcagc
    22921 tattggatca attttgttac tgttctttga tttattaatc tgaatgccgt tgttatccgt
    22981 ttttaaaata gcgttgttca ctgcatgggt gagaatcgta tttttattgt gaataacgtt
    23041 gccttcataa acttgttctc taaaagttcg ggtcggaacg tttagagtaa tcgttccttg
    23101 tcttacttct aacattgggt agttggcttt ttcagcttta ctgataagcg aattagcgtt
    23161 atacgggtca taacaaatcc ccatacattc caaatcattt tcttgaatca aatcttgaat
    23221 aaactgaaat acttggtcat agtccacgat tccactttct aattgagtaa tggaacactc
    23281 accagctttt tctagttctc gataaggcaa accgtcgcgc ttttctttgt cttgaagccc
    23341 atatttggtg gctacaaagc tatgtgaatc acaataaagt tcgccgttat ctagcggtac
    23401 gatccatgaa acgctagtta aatcatctgt tttagataaa tccactccga tataaacggg
    23461 cttgcctgta atatcttgtg gttctacttc ggttgcctgc cagtcttcag caatcatata
    23521 gctgtcctca ctcgcttgtc tccataaatt gaagtttttc accaatacag aattaaggtt
    23581 attttgtttc aaagcaaggt tcacatcatc ttgaatggtt ggaatcatta cttttttgat
    23641 ttcttcactt tcaaaaatcg gattggcttt gatccatttc tcttgatcgt gaatttcttc
    23701 gggatcgtcc aattcccata tcgctataaa gtacctgtcg gcttgttctt cgccttttag
    23761 cacacgatct aacaataagt attcctcgta cataggaacg tttaaatcaa gcccagcggt
    23821 actaataaca cacaataagc cgttcttttg ttgggtcatt cctgacttaa tgacgttgta
    23881 cgttttacga gtttttgctt cgtgccactc gtcaagaatt gcggttgttg gtgcaaaccc
    23941 atctagcgtg ctggtttcac tagccagtgc catagcgaac gaattagagg gcaagtgctg
    24001 aatcttagag ttcataattt tcagttgtgg tcttaaaaac ttactagact tgaccacgtt
    24061 tctaagtgaa ttagacagca tatcatagcc caatttagct tgtttcaaag cgttacttac
    24121 aaacaatact tgccttgctt ctgctggttc ttgttccata atcaacgcat tggcagccat
    24181 gccacttgcg atatacgttt tcccattctt acggctcata ctggtaaacg ctcgattgaa
    24241 tcgcctgtaa ttcccttctt tagtacgcca gccataaaga ctgcctagaa tgaatttttg
    24301 aaatcctaac atcttgattg ctttaccgtc cgtagatggt agcaactcaa tgaatttaat
    24361 cgctttggtg gcttgttctt catcgaacac ataagggaag ctatctgact tagaacgctc
    24421 caaatcgctt atatgacgct cacacgcttg gataattttc ttgggtgcaa tcagcttacc
    24481 tgataggact ttctcaatgt aatcattcat gaattcatca actctttgaa cgggtcttcg
    24541 ggttcatcat ttttaacctt gttcaaagct agttttgatc gtgattctaa ggtcatacct
    24601 agtgaggtgg cttgtttttt caaatcactc atagcttgtg attgaacccg aaccgctgga
    24661 ttggctttct ttactccgtt ttcgcatgtg ataaacgtac ctgattttct tatctcggct
    24721 tcggctgttt tcattcgagc aaaggctaaa caataggcta ccaatgaacc ataatctgct
    24781 tcactcaatg gataatcttt ttttaatgtt ggtacgatac gattccattc acttctagcg
    24841 ctcgctgcca tccagtcagg gggcgtgata tttattaatg gttgctgctt gaacaattct
    24901 tctctagcgt ccacacggat tgctttttct tcacttgata tattgccttt tgtatcgtgt
    24961 aatattttag ggtgtcccat gttgtcctcc tttcagacaa aagaaaaagg aacgtaagca
    25021 attaagctca cattccttcg tttttcgatc agaatttttc tttatctaat ttatctatat
    25081 aaatgatttt tccgttctgc atttgaatgg tcacttgacc aaaatgaggt aactcaatgg
    25141 tgcgctgttt ccctgtttta tcagtaataa caattgtatt tttcaataaa tctcctactt
    25201 tcattatata ctttttcact tgtgattaca agtgtaatcg ttatttgtaa ttacccttat
    25261 gccttttcac aacggttatt tgtaccaaaa tgagtaggca tcgattgtca gtaagtttca
    25321 cactatgccc ccttaactca aagggtactc ttttctcgtc tttgtctttc attggctgtc
    25381 ttggaattat ggtgtgcatg gcataaagaa cgaaaattag tttcatctaa ccgtctattc
    25441 caatcatctt ttagttcaat tttgtgatct acaacatcag ctttccttat caagccttct
    25501 tctaggcaat cctcacatac tggatgattt aaccgataca agtatgaagc ctttcgccat
    25561 gttctacttt gatagaattg aaaatattta ccttcgcttt gctttctttc ttcacgctta
    25621 attgccgttt gctttgcttt atgcttccca caatacttct gcctataatc aatcaatgtt
    25681 ttacatccag catgattaca ttgtttttta actgccatag attaccaacc aaagcgatct
    25741 cgttcgtcag aatcatcttg ttcccatttt gatttgtatt caatataagg cgaaactatc
    25801 ttcaagcacg ttccgtcttt aaaattaact ttagtgattt tatcttcctc aaaactaata
    25861 aagtctacat cttcttgagt gtttgaatac tcaatgtgtt tctcaccatc gaagtatcta
    25921 atactttcca tatgcataat tattctacct ccaataaatt agttatcaat tcatcatctg
    25981 ttagtagcaa agccaagaac ttatcatgga tgtttaaaaa caaattatcc tcattcgatg
    26041 taagctctcg ttgtctcgct cgatcatata ggttaagcag acaattaaat tcatccatta
    26101 gctcacctgt atcatcatag ttgccactat ctaccaatga tcccactact agcgattgac
    26161 accacgctac tgactcctgc agccaatcag gttcttcttc cattatcttt aatacttctg
    26221 gatcaaatgt ggaataatct tctgttacca atcccacact aaatgccagc gtgttcatta
    26281 gaaaccgatc catttcatct gtatattgaa agttcggtgc tttgtttgtt gaaataatat
    26341 tgagcattta ctttcctcct tgattcgtaa cacgtggtaa cacctacgta acaggtaccc
    26401 gttaccactc aatcccaacg gggacaaggg ttctagcgtt ttggtaacag gtaacgggta
    26461 aaatccagta acattattcc ccagcatatt agctaactat ttcaaaagtt ttaaggaaat
    26521 aaggtgttac cccgttacca aattgctgta tcccttgggg cagtaagggt aaactagtaa
    26581 cgggctaggt gttactgacc cgttactacc cgttactttt tgtactttcc agctcgattt
    26641 ttattttcat aggcatttga cttattgtaa aatcctcgtc gtttatttct ttttggcagt
    26701 tgcttccgtt cccaaccagg aatactgcta ataatcaagg caatcttttt agtttctgca
    26761 tctgatcgac cattcaataa atcctttgct tgtttatcaa aaacagcttc caaaatatca
    26821 gcggtgagta cgctatcaac taaataaaac tcttgttgca tagtagctcc attatagaaa
    26881 tgctgtaaat ttttctcatt acatttactg tcaggatagt tctgataata agaacgtctc
    26941 gcccacgctg gagcttcttc ccatttatac gggatttcca tttccaaata tttagtgatt
    27001 gcttctttaa ttggattctc catcttggca tcctcttgat agtcttttgc aatttctaag
    27061 acttctttat cttcatcggg actaaaatag attcgttgtc ctttatcaaa aagcaccttt
    27121 gcttcagcta acacttgaag aaaatattca tcctttgttt taaacggatt catctttggc
    27181 ttattcttac aaggcaaagg ataaaaacgg cggtttcctg tatcatcttt caaatactgc
    27241 ccatcattcg acgttccaat aaatacacat tgccttgccc aagctatgac attccgacca
    27301 taaggaggtc taattttatc gatcttggca ctgatgaagt tttttatctg ttcaatttta
    27361 gttttgttca ttgaagataa ttctccaagt tcaataatta cattgccttg aagctgcata
    27421 taatcatctt tcttttcacc tagcgaatct aatgaatcgg tgaaaaaatc atccgtgtaa
    27481 agtaatgctg gaacggtact cttaccaatc ccttgcggtc catccaaaac tggaacgatc
    27541 tcaaacttta ttcctggttt atagatacgt gcgactgatc cgactatcca acgttttgtt
    27601 acttctcgtg tgtaaaggtt gtcgtctacc cctaaaaggt caataaacag tgtttccagt
    27661 ctaggctgtt gatcccattt aactttttcg atacgttctt ttactgggtt gaaaacattc
    27721 tttctagcca catttactac tgctttgtgt attttgttgt catcaaaaac tacattgtag
    27781 ttttcttcaa tatatgccgt taaggcatcc tcaaaactgt cttcccattc tccagcggta
    27841 cgattgatcc aaggagaatc ttttaatagg tgaatgtatc cgctaaattc gttataagca
    27901 attgttccag ctaaattgct atcattggtt aaaatcaatt ctgcattcac aacactgtta
    27961 cttttaatct gatcgtcttt attgcatctt aatcgtgaca tccaggtttt caattcaatg
    28021 atattgtctt cacgctcgct ggcttcggga aagtctaaaa tactatctaa tttatgctta
    28081 tgagccatga gctttccccc tcaaatcttt tttaatcatc gatcgaataa tttgattcac
    28141 ttctttatct tttagcggtg gttcattgta cgcatttgct gtataaatca attctgctaa
    28201 agtttgatta tctacaccgt gccatagtaa ccaaccgcat aacttagtta aatgatcgtt
    28261 tctactacct tcgccagcgc cttgaacaat catatctaat ttttttgctg tccctgtttt
    28321 tgctacgggt cggtattgag tctttatttc ttgcggtttt tttagcagta ggttcactag
    28381 ccaatcggga gctggtgcaa tattatttag agatacgttt tcaataggtt catatgctct
    28441 cccaacactt cttgaaataa tcatagttgg cgctattgga actcctttag tgataatatc
    28501 aatcccgctt aaacttttac ctttatcatc acgcccgaag atatcagcaa atgcacctgt
    28561 ttcttgttgg ataattacac catcaggaat tttaaagaag aaatggattc ctttacctgg
    28621 tgtgatttcc gtataagtag aaggaaatgg atcatacatt gaagcaagtt ttttataatt
    28681 ctcaacgccg tttcgtgttt cagaatgttg atctaggtca actaccacca tttgatgatc
    28741 ttcaagcatt aacccaatat ttctagttgc atcttccgtc caccattcat taatttgatt
    28801 agaatcgtta gttgcatcat tccaaccact agagcctttt attggaattt tagttccagg
    28861 agataaggga taaattgacc atcccgactt ttgtaattcc gttgctgctt gataattatt
    28921 tgccaataaa cctctcctcc tttttcaaaa tactggtata aatcattcag acacctccat
    28981 gagcttcaac tgctcaagtt cgtatttcaa cccatcccga acaaattcag caccttttaa
    29041 ttgattctga tactttaccc atgtattaaa tgttttcacc cttgcttctt gcatatcagt
    29101 aatgtttaac catgcggtta aaatctgatt ttttagctcg ttgatttctc gacaaattat
    29161 tttgtgttct gcatgttttt tgagtacata caaaactgtt agtttttcat tgaatgaatt
    29221 tccaaagtag gttttcccga tattattttc tagttcagcg atcccttgat taatttcctc
    29281 taattcaagg ctatcgagtt ggtttaatag ttttgttttg tacatctact tatgccctcc
    29341 agttcttaat tgatagtcgt actcgtcata agcagtgaag agatataacg ctaatcctgc
    29401 aaagatagct atttttgcaa ttaatggtaa taagccgaaa attccgccga ttaaaatgct
    29461 tcctataatt aagtttcgtt tcataatggt tccctccgat tttttaatat ggtataatcg
    29521 gaacaaagaa tatcgggttt tatcccaatt aagttgcctt tatcctgtcc gccaagactg
    29581 ttatggataa ggctttttct ttgttctcga tcatgttaat gctcctaagt tgtctgcaat
    29641 tcagcagaac gattataaat tttctcactc actgaaaact gcaacccata tttttcttta
    29701 acagtcatta attctactgt ctcatctaaa ataggttcac gatcaaccaa catttcagga
    29761 gtcatatctt tcttttttac cattttggaa tgaccataaa tagttgatac agctttattg
    29821 gctatcacat tcgctttaat gtagtctact ggttttggtt cagtaatagc gtggcttaat
    29881 ttagtcattg cttctttttg gtgttctttg tctagcatgc ggaatacttg aaagccttca
    29941 agtcctgtgg actggcgtag ttctttgatg atgtcgaaaa tccagtcttg aaatgcctcg
    30001 gcttcttttt tacggctttt gaatgccatg cggtaaatat tttttttcgt tgatgatgtt
    30061 tacatcttgt gttctgccca atgaatcgat gaccttacta gtagtaaccc catctttagg
    30121 caaagagtga atggctctag taacctgttt aagacctaac gcttccgcaa catctttcgc
    30181 cactgcccac cattcatcat taatatcgac aaatcttata atgtgtccgt tccaaatttc
    30241 agttttcatt caacgtcctc cagttcaagc cctagcaagc tcgctaacaa ctcaacttgc
    30301 tgatacaaaa tttcttgata ctcttcaata gaaagtcgtt ctttaaactc tgcttgtact
    30361 aaatcgttca agccacgcaa tactccgctt accgttggtt cttgtggtag caatttttga
    30421 tagtccattt gttcgtacaa acattcaaac gtttcttcta gcgctttgcc ttccaagttt
    30481 gctaattctg ccgctgtgtc ttctgtaaga attgttgata atactttttc gattgcttta
    30541 ttttgtgtcg attccattat tatttcctcc tattaatccc tttgccccca cggcttgcgt
    30601 ttatttttgg tattcttcca taaagcgatc acattcatct tgtgaaattc ttacgactcc
    30661 atcaactata atcaacttta aaccgttagg aacatatttc tgcatcatag tattgtatga
    30721 aacgttcata tacttagcag cttgtgcctt gttcatatat tttggaaact ggtacttttt
    30781 ttctaataat gaaagtaacg ttccttgagt ttgctctact tgttgaacaa ctcgatcgtc
    30841 aataatttta aaagctggat catcaagcgt gagtttgtca atttttaatt ccatatttat
    30901 cccccctcgt tagcatacat ttctatacaa agattttaaa aaaatgcaag aggctcttga
    30961 atacatttct atacctctta taaactcaat ataccatcat accttttcat ttgcaagaat
    31021 tttaacatac ttttttgaac ttttatgttt cacacatatt taaatcgtga tatagtagag
    31081 aaaatatatg aatggtggtg aaaatgtggc tagaaaaaaa taccccgaag cagtggagac
    31141 aggacaaaga attagactgg ctagaattga aaagggactc tcaatggaac aacttggagg
    31201 taaattatcc ccaccggcca gtaaaggtgc cgttagtaat tgggaaaatg gctataatct
    31261 tcccaataac accaggctta aacaactttc aaaggtatta gatgtatcca ctacctatct
    31321 attaaacgga tcttatacat taagagatat gcatatgatg cccgaaatta agcgaaatgt
    31381 gcttgaaaag caattgaaaa aaactattga aaaaaataac tctataattg attctaatct
    31441 ttctaatgct atcaataaca tcgaagaaga taacctgact gtctcacaga agatttattt
    31501 aacaaacgca ataaaatatc ttaaaacagg atctcgaatt gaatttcctg acgggagtac
    31561 cccaattgct tatctcgcag ctcttctaaa tacgctttta cgatatgact cattaattaa
    31621 tgattcagaa aaaaatacag atgagaaaaa agagtatcaa gaattttatg aaaatgatct
    31681 tctaaaagag ttcacagctc ttgttactga gctaaataaa cactattcaa aataataata
    31741 aactgcctcc tccaaccgcc cccacggctt gctacactat ggagggaaaa ctattatggc
    31801 aacattcaag caatatgaaa ctaaaaaagg taaattttgg ctctatgaag cctatcttgg
    31861 aatgaacaaa atgactggca aacctgataa ggttcgtaga cgaggttaca aaacaaaaaa
    31921 agaagcccag cttgccttgt cacgcttgca agtggactac gataaaaacg gtattaaaaa
    31981 agcgaacaac gaaacgttta aagaagttta tgacttgtgg ctagaaacat ataaaaccac
    32041 tgtccgagaa gccaccttca tgaaaactga aatcaagttt agaaagtgga ttcttccaaa
    32101 atatggaaat cttcgtgtga atgaagtcac ggtaaaaaaa gcccaacaaa ttgtaaatag
    32161 ctgggctgaa actactgacc aatacaaggt acttcattca acttctaagc gtatttttag
    32221 ctacgctatc aatcttggga ttattgagac taatccacta gacaacgtat taatgcctaa
    32281 aagaaccacc actaagcata aagaaaaggt caaagtttac agtaaagaac aactgatcac
    32341 cttgtttgat tacctaaaca gcaaacctga aacttatcga aacgactttg ataaaacgtt
    32401 actacgcttt ctcttttata gcggggttag gatcagtgaa gcccttgcat tgaattggtc
    32461 agacattgat tttcaagaaa atacggtcac catcaataaa acactaagcc aatccaaaaa
    32521 tggctataaa atcagtgatc ctaaaactga ttgtagtgct ggggttcttc cactagatga
    32581 aagaacaatt tctatattaa aaaaatggca gattaatcag cggaaatata tgctgacact
    32641 tggtgtaact gatccgacta tgattttttg tggtatctat aaagaactaa tcacacacca
    32701 cgctatttat gcaagattga ttactatttg tgaaaaagct atggttccat ttctcggtgt
    32761 gcatgtagca agacacactc atgcttcaat gctcttggac gctggagcta ctatggtaga
    32821 agttcaaact agattacggc atacaaaaat tagtcagacg atcgattgtt acggtcactt
    32881 ggcaaaagaa acgaaagaaa agactgttga aaaattagta caacacttaa atattgacta
    32941 gatggcatac aatatggcat acagtaaaaa ataaaaaaag gagaaaccct atcatatcaa
    33001 ggtttctcct agctttatag tctattcttc ttcttccgcc atgaagttaa gctcattaaa
    33061 gataattgac gatagttggt atttcaacga tttttgaata gaactaatca ctttattaag
    33121 tctaattcac cttaagtggt tttttagtcg tattcaaaaa cgtattcaag ttgcgtggcg
    33181 tgcttttgat aagaaatcgt aagaactagt taatcaaacg acttataaat aatgtaacac
    33241 cactgattaa tcagctatat ccaaaaaaac aagcattcat tcagatacat caatctctat
    33301 agaatacaaa gctttcatta tctcaaacca ttaccacaac ttggcaagtc ggttaattga
    33361 agaacacctc attctccact tatagcagtg gagcaacaag ttgagctatt gttgcaacag
    33421 aagctaagaa ttctgtactc catttaagtt tttttagact atctaccgca aacctcagca
    33481 tagttttttt aggcttttct ttaatagctt catctttaat ggtttcgata tactcctgag
    33541 cctcttctac ctcttcttca ggtaaggaat cattcaaatt ctccaaaagc aaatcaagct
    33601 ttgataataa ttccgacata tcatcaatag aaacaatcat attattattg tggccttgct
    33661 gtatattagt gttgtaacta tttccaaata tatttacttt attatatatt gaattgttgc
    33721 tcatatagtc ctccagtcgt cctgctgcat tacttgcatc ttgtaacgtc acaaaaaaat
    33781 ctctcccatg ttggtaaatt gattcaacga tttcaaattc tggatttcca tattcgcgcc
    33841 attcgtattc ttccttaaag tattttccgt aactatggtt aaattgcaca gaatttttac
    33901 caattttcct tagaagtttt atctctctct cagaaataaa attttcctca aaagctgatt
    33961 caactatctg aaaatcacta agaaatgttc ctaaacagaa gattgattca ttaatagttt
    34021 gttgaaaatt ccatggtatt ttgttccaaa aaccattctt aattaaagcc tctttttttt
    34081 caacaatctg tgattttaca ttttctgtat atatcttcca attattgacg tcttccttaa
    34141 tgtccgaaat cgattgacct tcataagaag tccctccacc aaaaagtcct tgaaccattt
    34201 tatctcatcc tctctataat aattactcta aatccaatac ttgctacaaa tgatagcatt
    34261 tcgtgtggta acatgattgc catcgttcct gttttattta ctctgacttc aatacttggt
    34321 ttgttgatga aattttacca attgattcca tcgatcgttt catggaatct gtatttacta
    34381 ttttcattga ttgtagagat tgatttattc cctctgttgc acttctgata cctttagtat
    34441 caaagttttg catgattgct cgctgagctg ataaagctga taatgtttga cctattgact
    34501 ctatcgacct cttcatggaa tctgtattta atatttttat tgattgtaga gattgattta
    34561 ttccctctgt tgcccttctg ataccttcag tatcaaagtt ctgcataatt tcttgctgga
    34621 ctgataatgt ttgacctatt gactccatcg actttttcat ggaatctgta tttaatactt
    34681 tcattgctga tagagactct gacaatttag taacacctgc taaatcgata ttactaaaat
    34741 aatttccaat atatccttca aaatcatctg acatatcact aaggtcctca ccagcaatat
    34801 ctccagccaa tattttttga aaagctttct ctagttgatt tgtatatctc ttcaatttct
    34861 ttattaattt ttggtataaa gccatttcta tttctttgtt atggcttaca atattcctac
    34921 cttctctgat taaactcatg tctgtttgaa tttcttctag cttaacatcc atatatttac
    34981 taaatacttc actccacaaa gaatatgaat cagatatagt gtgactgtta tcttgaaata
    35041 acttcaataa ttgatcctga gatatttctt tataccttac aatattctct tcattttctt
    35101 gtgtaactaa aatataattt tctccaaaga gatattcttc aaaatcatat aaatctatcc
    35161 attgtaaaag attctttgag tcaaactttc ctattgcttg tatttgtttt ctttttattg
    35221 acgaaatgat ttcatttgga attgattcct ctgcccagtt atcagaaaaa tatgccggag
    35281 ctaataaata gattaatttt cttaatgccc attcgtaact atgcaaatac ggtaatagtt
    35341 ttttcgaaaa aaacaacgca tttgtatcta aaagggtagt gtagtttaat tttgatttac
    35401 ttgcttcaat attgacctta gctaggaaaa tttgaaatat atgtacctct tttattccta
    35461 tcttaccagt cttattatat tgaaatacta cgtgtaacga atcattatag ttctttatgt
    35521 caaagccaaa tttataattt ttttcctgct cctgaaatct taagatagaa tcactaatat
    35581 atggtgacaa cactttcaga aaggtttctt cgatattttc cattcttcct tttttaggat
    35641 aatataaata ttcaacaact tgcttactaa tatgtcatca acttctttcc tttcaacaat
    35701 tttaattttc ctattaactt gatatttgct acataagcca caactagcaa acagaaagag
    35761 aactttttct ttaccttgat aactgtgtta attactggtt tcttttttct atttctttat
    35821 agtattgttc ttctactttt aatatttttt taacttctct ttttatgtca gcatacatag
    35881 atatactgtt gtcctttcgg ttgaagcagt gataattctc accatcaaac tcataaaaaa
    35941 gcatatcact tgagcttcca tctgaattaa ttaactgaaa tttggtattg agtagtaatt
    36001 catttttctc gtcattcaac tttattaaaa taggtttgat ctcttttaaa taattgaaat
    36061 atatttcggt tattactaaa ttaaatttcg ctactgtata aacataagag atacgtgggg
    36121 aatctctaaa ttggttatat atatctttcc ttaaaacatc taactccttt tttaattgtc
    36181 cgttaaaatc aaaatgaggc attgctagaa tttcatccaa atcaaagtac actctttgtt
    36241 gatgaatatt tactggcaga tgaccatgct gagaataatt tctcatatgt aataaaaacc
    36301 tatacgaaaa atgctcatca taaggcttac tcagaatttt attcttaaaa gatttaaaat
    36361 ctttttctgc taaaaagttt ttcatacaaa cttcaatgga ttcagtcaaa gttttggctg
    36421 aactaattaa gtttatc
//
LOCUS       DAXWLF010000008        35602 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXWLF010000008 DAXWLF010000000
VERSION     DAXWLF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.008"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            212..1123
                     /locus_tag="ACGE4U_000285"
     CDS             212..1123
                     /locus_tag="ACGE4U_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8392478.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1379..2296)
                     /gene="rnhC"
                     /locus_tag="ACGE4U_000286"
     CDS             complement(1379..2296)
                     /gene="rnhC"
                     /locus_tag="ACGE4U_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFF8392479.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2498..2935
                     /gene="zapA"
                     /locus_tag="ACGE4U_000287"
     CDS             2498..2935
                     /gene="zapA"
                     /locus_tag="ACGE4U_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFF8392480.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            2993..3540
                     /locus_tag="ACGE4U_000288"
                     /pseudo
     CDS             2993..3540
                     /locus_tag="ACGE4U_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            3655..6015
                     /locus_tag="ACGE4U_000289"
     CDS             3655..6015
                     /locus_tag="ACGE4U_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFF8392481.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6102..6416
                     /gene="trxA"
                     /locus_tag="ACGE4U_000290"
     CDS             6102..6416
                     /gene="trxA"
                     /locus_tag="ACGE4U_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFF8392482.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6598..8376
                     /gene="uvrC"
                     /locus_tag="ACGE4U_000291"
     CDS             6598..8376
                     /gene="uvrC"
                     /locus_tag="ACGE4U_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFF8392483.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8521..9420
                     /locus_tag="ACGE4U_000292"
     CDS             8521..9420
                     /locus_tag="ACGE4U_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8392484.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9413..10675
                     /locus_tag="ACGE4U_000293"
     CDS             9413..10675
                     /locus_tag="ACGE4U_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8392485.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            10838..12736
                     /locus_tag="ACGE4U_000294"
     CDS             10838..12736
                     /locus_tag="ACGE4U_000294"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFF8392486.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(13035..13754)
                     /locus_tag="ACGE4U_000295"
     CDS             complement(13035..13754)
                     /locus_tag="ACGE4U_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFF8392487.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    13944..13991
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            14091..14423
                     /locus_tag="ACGE4U_000296"
     CDS             14091..14423
                     /locus_tag="ACGE4U_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392488.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14410..16413
                     /locus_tag="ACGE4U_000297"
     CDS             14410..16413
                     /locus_tag="ACGE4U_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFF8392489.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16432..16902
                     /locus_tag="ACGE4U_000298"
     CDS             16432..16902
                     /locus_tag="ACGE4U_000298"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFF8392490.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            16923..17507
                     /locus_tag="ACGE4U_000299"
     CDS             16923..17507
                     /locus_tag="ACGE4U_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFF8392491.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17522..18508
                     /locus_tag="ACGE4U_000300"
     CDS             17522..18508
                     /locus_tag="ACGE4U_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFF8392492.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            18498..18809
                     /locus_tag="ACGE4U_000301"
     CDS             18498..18809
                     /locus_tag="ACGE4U_000301"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFF8392493.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            18831..20612
                     /locus_tag="ACGE4U_000302"
     CDS             18831..20612
                     /locus_tag="ACGE4U_000302"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFF8392494.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20605..21981
                     /locus_tag="ACGE4U_000303"
     CDS             20605..21981
                     /locus_tag="ACGE4U_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFF8392495.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            21988..22623
                     /locus_tag="ACGE4U_000304"
     CDS             21988..22623
                     /locus_tag="ACGE4U_000304"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFF8392496.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22653..24008
                     /locus_tag="ACGE4U_000305"
     CDS             22653..24008
                     /locus_tag="ACGE4U_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFF8392497.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(24200..25594)
                     /locus_tag="ACGE4U_000306"
     CDS             complement(24200..25594)
                     /locus_tag="ACGE4U_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFF8392498.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            25686..26657
                     /locus_tag="ACGE4U_000307"
     CDS             25686..26657
                     /locus_tag="ACGE4U_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFF8392499.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            26654..27436
                     /locus_tag="ACGE4U_000308"
     CDS             26654..27436
                     /locus_tag="ACGE4U_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFF8392500.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(27605..27976)
                     /locus_tag="ACGE4U_000309"
     CDS             complement(27605..27976)
                     /locus_tag="ACGE4U_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8392501.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            28110..28553
                     /locus_tag="ACGE4U_000310"
     CDS             28110..28553
                     /locus_tag="ACGE4U_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8392502.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(28596..29405)
                     /gene="yidA"
                     /locus_tag="ACGE4U_000311"
     CDS             complement(28596..29405)
                     /gene="yidA"
                     /locus_tag="ACGE4U_000311"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFF8392503.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(29424..30791)
                     /locus_tag="ACGE4U_000312"
     CDS             complement(29424..30791)
                     /locus_tag="ACGE4U_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8392504.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(30775..31605)
                     /locus_tag="ACGE4U_000313"
     CDS             complement(30775..31605)
                     /locus_tag="ACGE4U_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFF8392505.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            31768..32205
                     /locus_tag="ACGE4U_000314"
     CDS             31768..32205
                     /locus_tag="ACGE4U_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFF8392506.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            32281..32901
                     /gene="rpoE"
                     /locus_tag="ACGE4U_000315"
     CDS             32281..32901
                     /gene="rpoE"
                     /locus_tag="ACGE4U_000315"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFF8392507.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            33090..33596
                     /locus_tag="ACGE4U_000316"
     CDS             33090..33596
                     /locus_tag="ACGE4U_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFF8392508.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            33613..34512
                     /locus_tag="ACGE4U_000317"
     CDS             33613..34512
                     /locus_tag="ACGE4U_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFF8392509.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            34599..35483
                     /locus_tag="ACGE4U_000318"
     CDS             34599..35483
                     /locus_tag="ACGE4U_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8392510.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        11697 a         6147 c         7599 g        10159 t
ORIGIN      
        1 attgtttgta gaatgaagga aactatttat tgggtagatg aatcaccacg aatagtcaaa
       61 aggagatttc aggatatgcc aaaaatgaaa aaaatcattt tagcagtaat gttattttct
      121 acggttatat cttttacagc cccttttttg cgtacttaag ctgattttaa gttttgaccc
      181 ttagtctgga tctacgaggt gagagtcaag aatgaaaaaa ataattggag cttttgtatt
      241 tgcaatcatt atcattatta tctgttttca aatagatgtt tttggtcaaa aaaagatagt
      301 tggacaaacg gtcagcagta cgaacagtac ggttacaaag acaaaggaat catttgatga
      361 tacagaacaa gagacattaa tcaaaatcgg aaaaaatgac cgtacactat atacagatga
      421 ctcgctaact accccacttg caactataaa cggcggagag ctggcggagt ttctttctga
      481 aacaaaaaat gcttatcaga tcaagacaaa tgatggctat actggatatt tagccctcga
      541 agatggaacc aaagtcacaa agaatataca aacaaaaccg gaagagctat ctgatgcagt
      601 gattgtatta gatcctgggc atggcggaaa tgatactggg gcattaagca atgatgaaca
      661 aacagaagaa aaagatataa cattaagtac agccataaaa gtaaaaaagg cgctagagga
      721 tgctggagca acggtttatt tgacccatac tacagatgaa ttagtacagc ttggagatat
      781 atgcgactac agtgaagaaa aaaaggcaga tgtctttatc agtttacacg cagactctac
      841 ggaatatgcg aatgaagcaa ctggaatcac gacatattat tactatggac aagaggaaac
      901 attagcacag acaattgctg actccttcac tgatctgcct ctggattcaa gaggaatctc
      961 cactgggaac tatcaagttc ttcgagaaaa tcttcaacct tctattttaa tcgagatggg
     1021 ttatatgaat aatgatagtg atttaaagga attagtaaca gacagttatc agcagcagat
     1081 tgctgcaagt ttgacacaag cattgaccac ttattttcag taataaataa gcccagccaa
     1141 acaaaaatag ctcctcatat ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt
     1201 tgaacaccgt ttaaggaaga ggttgtgagc ctgccgttta actccgcgtc acaaggagca
     1261 atttcaaaaa acagcttctc atgtttgatg actattgtga cttgtgaccg aggagttggc
     1321 atgagaacac cgtttattcg gaacaaggga ctgtgacatg acttttgtca cagttccatt
     1381 atttttttag cagcttcaaa gctttttctg tattggcaaa atgaagtttg gcatattttt
     1441 caagcatagg catgccgcct ttctccaata gttttgccgc tacttggtca gatttcgttc
     1501 cagctcctga tggaagggtg attccagctt cagctgatgc tttgtgtaac tcttccaaaa
     1561 agctggcacg actgatgata gaagcagcag caacagctaa atgatattgt tctcctttag
     1621 taacgaagta cagtttttct tgtaattgat ttttttctaa acgcgcatat tttttaaaat
     1681 ttgcttccgg cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc tcgatttttt
     1741 ttagcagcaa atggatcgct tgattgtgca aagcgacttt catccgaacg gcattgtatt
     1801 ttggttggat ctcattgtat ttttctggag ataagacaag cagtttatag gggatggttt
     1861 tttttaagat gtcagcaagc tggatgattt ggctatcttt taactctttt gaatctctaa
     1921 caccaagttt acgtaattct gcaagctgat cacgtttcac ataagcagca catactgtta
     1981 gcggaccaaa atagctgcca tttccgacct catcactacc taaaacggat aattgactga
     2041 tattttttgg caaagaagag gctgtttttg ctggcttggc ttgtttatcc tgtgcctccc
     2101 cccatttatc agcttctatt gctgcattcg taccttgaaa catcactttt ccggatgtat
     2161 aagcggtaat ggtcgtcccg tctttttttg cagcaaagct agtataagga atctgtttat
     2221 ttaacaaata agagcgatag tatgctttca tttgactaag ttggtcgttt gttgctttga
     2281 taatgacgtt gctcataaaa ttctcctcgt ttttatacat aaaactatac cgcaactcac
     2341 gacttttcgt tttctaagag attgcgagac atagcatttt tttaaagttt atcatataat
     2401 agttaagtat gtgaaaaata tttgtggaga acggtgaaag aaccaacttc atggtacact
     2461 aaataaaact ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa aaacacgcta
     2521 caaagcagtg attgccaacc aaacatatac tattatcgga cgggaaacca agcatcatat
     2581 ggatatcgta acgaagctga tcaatgaaca gttagcagaa ttgaaacaat tatcaccaca
     2641 aatggataat gaacaagcag caatcttgat ggctgtcaat gcgctttcgg atcaattgaa
     2701 gaaacaagaa agaatcttgg agctggaaga agaaacagca gaattaaaga aaaaaatgat
     2761 caaatttaca gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga acgaagctcg
     2821 ggaagtattg aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg tggaagcaca
     2881 acagatttta aacgagaaac gaaaaggaca aattaaacaa aaatcttcta attaagatcc
     2941 ttttatcaaa ataagaaaaa tttaaaaaag ttagaagaga aaggaacgta gcatgctgag
     3001 tttgctgatt ttatttttat tgctaattgc gtttttttct ggagcacgtc gcgggtttgc
     3061 tttgcaaatc atattcgcga ttggatatgt attgtcattt attgctgccc agcattttta
     3121 caaaccatta gcaagtcatt tggaattata cattccatac ccagctgtca ccccaacatc
     3181 aaagttagca ttttttgatc aggtatttgc ttttcaccta gatgaagctt tttatgcagg
     3241 aactgctttt ctgattattt tgctaattgg ctggctgctt acccgatttg tgggggtttt
     3301 tgtccatgga ttgacgtatg tgccgatctt gagacaggta gactggattg caggaggcat
     3361 cctgtcacta atcatggctt acgttactat atttttgatt ttgcagttac ttgcctttgt
     3421 tccattagat gtggtccaaa atcaattcgg cgagcatagt ttcgctcgtt ttatcgttga
     3481 gagaacgcca tttcttacca acaagatgta tgagctttgg taacgaatat tttgaattaa
     3541 ggaaaatagt gctggaaaat atttcgtttt ttgtacagag tagtttattt aagaaggctg
     3601 cgtagcgtat gtccggcaca gctctttttt catagtgaaa gataaggtga taaaatgaat
     3661 aaccgaatcc ttgaaacatt ggaatttgaa aaagtcaaac agatggtcag acaatttgta
     3721 gtgactgcac aaggaaaaga agaactggca gaattggtac cagtgagtga gaaacaaacg
     3781 atcacaaact ggcttcaaga aactgaggat ggattgaaag tgcaacgtct gcgaggcggg
     3841 atccctatac ctaaacttga aaatatccgt ccccatatga aacggatcga gatcggagca
     3901 gacttgaatg gagtggagct tgcacaagtt tccagagtat tgtctacgac aagcgaattg
     3961 aatcgtttca ttgatgatct ttctgacagt gaaatcgaat ttgcacgttt gtatatgtgg
     4021 gcagatcagt tagtcacgat ccctgttttg agccgccgtt taaaggaagc aatcgatgaa
     4081 gacggacgcg tgactgatga cgcttctcct gagttaaaga gcataagaca gaacattcgg
     4141 cgaagtgaac aagctgttcg tgaacagtta gatgggatcg tccgtgggaa aaatgcaaag
     4201 tatttaagtg atgcgatcat caccatgcga aatgaccgct acgttatccc agtcaaacaa
     4261 gaataccgcg gtgtatttgg cggtgtagtc cacgatcaaa gtgcttcagg acagacttta
     4321 tttattgaac caaaacaagt ggtggatttg aataatcgtt tgcgtcaata ccaaatcgct
     4381 gaacgaaatg agattcaacg tattttaagt gaactttctg ctgaacttgt tccccatcgt
     4441 caagaaatca tccacaatgc ttatgtgatt ggtaaaatgg atctgatgaa tgcaaaagca
     4501 cgatttggta aagaagtaaa agccatcgtg ccgggaatca gtgaagacaa tcatgtcgtc
     4561 ttgaaacaag caagacatcc actgatcgat caggaaaaag tagtttctaa tgacatcaca
     4621 atcggaaaag actatcaagc catcgtcatt acaggaccta acacaggtgg gaaaacgatc
     4681 acgttgaaga ctttaggtct gttacaactg atggggcaag caggattacc gattcctgca
     4741 ggtgaagaaa gtcaaatcgg tatttttgag gaagtatttg cggatatagg agacgaacag
     4801 tcgattgagc aaagtctatc gaccttttct tctcatatga cgaacactgt agatatttta
     4861 tcaaaagtca atgaaaaaag tctggtatta tttgacgagc tcggtgcagg aacagatccg
     4921 caagaaggtg ctgctctagc aatcgcaatc ttagatgatc ttggtaaaaa gtctgcttat
     4981 gtgatggcaa caacgcatta tcctgaattg aaagtttatg gatacaatcg ggcaaatacg
     5041 attaatgcca gcatggagtt cgatgtggat acactaagtc caacgtatcg tttgctgatt
     5101 ggcgttcctg gacgaagcaa tgcgtttgaa atctctagcc gattaggtct tgatacagaa
     5161 gttattgatg aagcgaagca gttgatgaat gatgagagcc aagatttaaa cgaaatgatc
     5221 acagatttag agaatcgccg gaaaatggca gagaccgaat atctggaaat gcgtcatttt
     5281 gtttctgagg cacaagaact tcacgatgat ttgaaagaag cttacagcta tttctttgaa
     5341 gaacgtgaaa aagaaatgga aaaagcaaag aaaaaagcaa acgaagtcgt gtcagaggcg
     5401 gaagaaaaag cagaaaaaat cattgctgat attcgtaaaa tgcaacagca gattgggcaa
     5461 ggaaatgtga aagagcatca gttgatcgat gcaaagaccc aattagcaaa tctacatcaa
     5521 gaagaaactt tgaagaaaaa taaagtattg aagaaggcga aagaacaaaa aacgttgaaa
     5581 ccgggagacg aagtgttggt tacgacttac ggccaaagag ggacgctttt acgtaaaaac
     5641 gggaatcagt ggcaagtaga aatcggtata ttaaaaatga atgtgtccga agacgagctg
     5701 acgcctgttg ctcctcaaaa agagccgaca caacgagtta tccatgctgt acgatctgaa
     5761 tctagtagtc acgtgccgaa tcaattggac cttcgaggaa aacgatacga agaagcgcta
     5821 agtgaggtag atcaatattt ggattctgcc attctagctg gttatcctca agtgactatt
     5881 gtccacggaa aaggaacagg cgcacttcgc aagggaatta ctgattatct taaaaaccat
     5941 cggagcgtca aaagctttga gtttgcaccg gcaaaccaag gcggaaatgg tgcgacaatt
     6001 gttaaattta agtaaagctt actaaaggta agcagatggt tcgctatcca agtatagaaa
     6061 tgctatacta atcatagatt ttaaaaatgg aggtctttga tatgtcacaa gtaattacag
     6121 ataagacatt taatgaagaa acagataaag gacttgtatt gattgatttt tgggcaactt
     6181 ggtgtggtcc atgtcgtatg caagcaccga ttttggatca gttggaacaa gaatatgatg
     6241 aagaagaatt cagaattgct aaaatggatg tggatgaaaa tccagaaact cctcaacaat
     6301 tcggtatcat gagcattcca acattaatgt tgaaaaaaga tggtcaagta gtagaaaaag
     6361 ctgttggtgt tcattcaaaa gaacaactac gtcaaatgat cgatcaatat ttataaaaac
     6421 ttacagcaaa aataagctga gggtgggact gaatccagtt ccgtcctcgg tttttttgcg
     6481 gttgagttaa ggttattcaa attaatattt tttgagtata attacgttgt caggctggat
     6541 tgtttttttg acagacatgc tataataact ttaattgaat atgaagggga gtaccagatg
     6601 aacgaacgaa tcaaaaacaa attagcttta cttcctgatc agcccggctg ttacttgatg
     6661 aaagataaaa atggaacgat catttatgtc ggcaaagcca aaatattaaa aaatcgtgtc
     6721 cgctcatatt ttactggcag ccataatacg aaaacggaac ggcttgttag tgaaatcgaa
     6781 gactttgaat acatcgttac agaatcgaat atcgaagcat tgctcttaga gatcaatctg
     6841 ataaagaaaa atgatcctaa atacaacatt atgttaaagg atgataaaac ctatcctttc
     6901 cttaagataa cgaatgaaaa atatccccgt ttggtgatta ctcgtaaagt actaaaagac
     6961 aaagcactgt attttggacc atatcccgat gtcggtgcgg cgaatgagac aaagaagatc
     7021 ctcgatcgat tatttcctct gagaaaatgc aaacctagtc agacgaagga gccttgtctt
     7081 tactatcatt tgggacaatg tctgtgtcct tattatttcg acgtagatcc gaaagtctat
     7141 acgaaaatcg ttgatgaagt gaaaaagttt ttaaacggag gacacgaaaa aatcgagtca
     7201 gagatccatc agaaaatgga aaaagctgct gaaaacatgg agtttgaaaa agctgcagaa
     7261 taccgagacc agatccgtgc cattgagaca atcatgactc gccaaaaaat gacaaatact
     7321 gatttactgg atcgagatgt atttggttat gcgattgaca aaggatggat gtgtgtccaa
     7381 gtgtttttcg taagacaagg aaagctaatt gaacgagatg tttcaatgtt tcctttctat
     7441 aacgaagcag aagaagattt cttgacttac ctcggtcagt tttatcagga aaatgagcat
     7501 ttcattccta aagaagtctt gattcctgat acgatcgaca agcccagtgt agaagctttg
     7561 ttagctacga aagtgctgca accaaaacga ggagaaaaga aaaaattagt tgaattggcg
     7621 aataagaatg cctcagttgc tttgaatgaa cggtttgatc tgattgccag aaaacaggaa
     7681 cggacgatcg gagcagttga acgtttagga aacgcgatga atatcccgac cccaatacgt
     7741 atcgagtctt ttgataactc taatatcatg gggacgaatc ctgtggctgc gatggtcgta
     7801 ttcattgatg gaaaaccggc aagaaaagaa tatcggaaat ataaaataaa aacagtcaac
     7861 ggaccggatg attatgcatc aatgagagaa gtcatctatc gcagatattc acgagtgatt
     7921 cgcgaagggt tgcctttacc tgatttgatc ttgatcgatg gaggaaaagg acaagtagat
     7981 gtggcaaaag atgtcttaga aaatcaatta ggaatcgata ttcctattgc tggcatggca
     8041 aaaaacgata aacataaaac aaatgagttg ctattcggta tagatttggc tgtagtaccg
     8101 cttgagcgaa attcaccaga gtttttctta ctccagcgaa tccaagatga agtccatcga
     8161 ttcgcaatca ctttccatcg gcagctacgc agtaaaaaca gttttgcttc ccgattagat
     8221 gaaattgaag ggttaggacc aaaacgaaaa aaaatgctgc ttaaagagtt caaatcgttg
     8281 aaaaatatca cagcagccac gacagaagaa ttacaagaaa tcggactgcc gaaaaatgtc
     8341 gcacaaaacg tatttgataa attacatcag gaatgaggaa agggcacttt acaaagaaag
     8401 taaagtgtcc tatcatttta tgtacaacaa aaagagcgtt tacgttttgt tggtaaataa
     8461 taaatcgctt acgtctggtg tctaaggata tatgtgattt cttgaagaag gagtgaagaa
     8521 atgttagaag tcaaggatct tgttaaaact tttgggaact acacagctgt cgatcatgtc
     8581 tctttccaaa tccccgatgg aaaaatcatg gggctaattg gccaaaacgg ggctgggaag
     8641 acaacgactt ttcgtttgat cttggatttt ttgacacaag accaaggaga agtgttatgg
     8701 aatggccatc atcttgggga gaaagattat gatgtcatcg gttatcttcc ggaggaacgg
     8761 ggactttatc cgaaagtaac gattcaagac caactgcttt attttgcaga acttagaggg
     8821 aaatcaagaa aagagattga accaaaaatc gatttttgga tggaaaaatt tcaggtaaaa
     8881 gggaaaaaga cggataaagt aaaatctttg tctaaaggaa atcagcaaaa agtccagctg
     8941 atcgccacgt taatccatga acctaaattg atcattttgg atgagccatt cagcgggtta
     9001 gatccggtaa atgctgaatt gctaaaagat ggaatattgg aattgaaaaa acatggttca
     9061 tgtgtcattt tttctagcca caatatggat aatgtagaaa aaatctgcga tcatttgatc
     9121 atgttgcgaa acggaaaaat ggttttgaat ggaaaagtac atgagattcg tgaatcattt
     9181 ggccgtacga agttattctt ggaatctggt ttaagccaac aggaagtaga agaaatcgaa
     9241 ggcgtgaaaa aagtgatcct tcatgaagac aatacgttag agattacctt agaagatcct
     9301 gaagctggga aagaaatctt tactcgtgcg actcagttcg gctacattcc tatgtttaac
     9361 cagcaaccgc caactttgga agagattttc aaattgaaag caggtgaaat cgatgcgtaa
     9421 gttttggatt attaccaaag atgtctattt aaaaaatgtt aagtcgattt ctttcttgat
     9481 catgatatta gtccctttta tcatgctggg tatcatttat gtagcaggaa actttgctca
     9541 acaaaatagt gatgtcaata aaatcggcgt attggctgat aatgagcaaa tcagtcagca
     9601 aatgagccaa gtcaaaacag atgattttga gttcaaagca gttgattctg aagaaaaagc
     9661 acaaaaagag ctttcggaag aaaaaatcga tgcgtattta gtagtctcaa ctgagaatga
     9721 taagataaat gggatgcttt atagcgaaaa ttcattagga caaagtacac aactactgat
     9781 acaacaacaa ctgactggct tgcaatcgat gcttcgagca agtcaattag gcatttcacc
     9841 agaagaagta gctagcttga gccaacaagc aagtttgact cgacaaaaag tcaattttga
     9901 ttcaaaagga aaaatgacag ttggagaaga taactcgaca gtccaatatg tcgtaagtta
     9961 tgtagcaacg attgtcttgt tcattattat tttgacgtat gcgcagatca ttgcacaaga
    10021 gatcgcttct gaaaaaggca cgcggatcat ggaagtgatc ctgtccagta cgactgcaca
    10081 aaagcatttt tacggaaaac taacaggtgt tttacttgtt gcattaacgc aaatggcatt
    10141 atacggtatt atttttggta taggatacag tcaattcaaa gatatggaaa tggtcaaaaa
    10201 tatattagac ggtatctcat tggataactt gattggacca ttcttgtggt attctcttct
    10261 attcatgatc tttggcattt tgattttttc tgttttagca gctttatgtg gctctttggt
    10321 caacaaagca gaagatacag caaaagcaat tttgccagtg acttatctat ctctaggcgg
    10381 atatatgctt ggattgatac taggagcttc agatccaaac aatatcgtta ttcggatcac
    10441 ttcgtatatt ccattcttat cttcatatat tatgccggtt cgcttagcaa atgagacagt
    10501 cggtatgggt ggagcattga tttccttgtt ggtacttgta gccgtaacat tcttattgat
    10561 gatcttgtct gcaaacatgt acaaatcgaa tgtactcgtg tatagcgaag gcggattatg
    10621 gagttcattg aaacaatcga tttcaattat gagaaatgaa cgaaaaaaag cataaaatat
    10681 agaaaaatat agaaggaagc tggacattta ttgttcccag cttctttttt gtatagacct
    10741 atttctattt ttttattata ttggatatat atattcattg aaaatagagc ataacaactt
    10801 tataatgttg agtataccaa tttttggagg cgttttaatg gaccgctatt tagatttact
    10861 ttcagagaag ttccctacaa cggaagatgt catcacggaa atcatcaatc tcgaagcaat
    10921 tttgcagctt ccaaaaggaa cagagttatt tttgagcgat atacatggcg aatttcctgc
    10981 ttttgatcat attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt
    11041 ctctgaacaa atgacggaaa cggagatcag ccaattcact atttttacgg cttatccaga
    11101 atatgtgttg acgactgatt ggtacaaaaa acaagataaa tcccagctga tccatcgttt
    11161 gatcgatctt ttacggttta cgacagtcaa atacactcga tcaaaagttc gaaaaggatt
    11221 gccgaaagaa tatagctata tcattgagga actcttgtat atcgatgatc gtatcaacgg
    11281 gaaaaaagac tatgtaaaaa aaatcattga acaacttgtc ttgatgaagg aagaagaaaa
    11341 atttttaaca aaacttgcgc agacgatcca aaaatctgtc atcgatcatt tgcatatcgt
    11401 gggtgatatt tttgatcgag gaactcaagc agccaaagta atggatcgaa tcatggattt
    11461 ttcctctgta gatatccagt gggggaatca tgatattttg tggattggtg cgtattgcgg
    11521 ctctgaggca tgtttattga cgcttttacg gattgccgca cgctacaact atttatttga
    11581 attagagaaa gaatatggat taaatttacg tccattattt tcttttgccc atcaaaccta
    11641 tcagaagaat ccagtattta cccctaaaaa caggaaagat ctatctgaaa gagagcaaga
    11701 agaattagaa aagatccatc aagcactttc tatcctccaa ttcaaatcag aacatcagtt
    11761 gttgaatcga cgacctgaat ttaaaatgga cgatcgtaaa ttgttggaaa aaatcaacta
    11821 tgaggaatca acgatcgttt tagaaggtca gctctatgag ttgaaaggga cttgttttca
    11881 aacgatccag ccagatgatc ctaaaaaatt cttggaagag gaacaaaaag tgatcgatag
    11941 tttgatgtat tcattccagc actcgttaag aatgcaaaaa cacatgacgt ttttgatcga
    12001 aaaaggggga atgtatctca cgaacaatca gcatgtactg ttccatggct gtatacctgt
    12061 agatgaaaag gggcagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact
    12121 gctgggattt tttgaaaaac agataactga aagcgcaaaa gatttgactg caaatgaaga
    12181 tttggcgacc gatttgattt ggtatgcgtg gagcgggcca ttttcgcctt tattcggcaa
    12241 gagtaaaatg gcaacttttg agcgctattt tctcacagag cctcatacgc acgtcgaaaa
    12301 aagcaatccc tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttggcaga
    12361 attcgatgca aaagaagaag gttcagcaat catcaatgga cacacgccag taaaagtaaa
    12421 aaaaggagag tcaccgatca aagcaaacgg aaaagcattt gttattgacg gaggtctatc
    12481 caaagcttat caaaaaacaa ccgggattgc tggctactca ttactatgca attcttacgg
    12541 tttccaaatc gtgacacatt atccgttctt gcctattgaa caacaatttg caaaaaaatc
    12601 tgatcaaacg aatgtaaaaa aagtgatcga gcagcagctc ccacgcaaac ttaatcggaa
    12661 tacgacaaaa ggaatggaat tacagcaaca gattactcaa ctccaaaggc tgttggatta
    12721 tcgaaaggac gattgagaaa aatgctagcc aacattgctc aatagactaa aaagcgactg
    12781 caacaaaagg cgtgtcgcag tctccttcgg aattagctca aaaaacggta aaatattaga
    12841 agctattttt ctgtttattt ctctaatact caggtcaggg cgctgatttc acgacctctt
    12901 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc aacaaacatg
    12961 agaagctgtt ttttgaaatt gctccttgtg actcagagtt gaacggcagg ctcacgacct
    13021 aatcagaaaa acgattaaaa ttcattcaca aagaaatcga ataaacgctg ttcagatgaa
    13081 tctacacgat gagaaagttc cggatgccat tggacaccaa ggattttgtg catattatct
    13141 ttactttgga ctgcttcaat gagtccatcg ttggaaaagg cgatggcttt taacaaagga
    13201 gaaagatctt tgactgcctg atgatgatag gaattcacaa agtatgaatc agggagaagg
    13261 tcacttaata aactgtccga aacgattttg acttcgtgag ttgcaaactg cggggcagtg
    13321 ggctgttgtt cgtgttttac ggaccatttt ggatataatg aaagatcttg ataaagtgtt
    13381 ccctccaaca cgacattcaa tagttgaagt cctcggcaga ctgcaaaaat cggttttctt
    13441 tgtttcaatg cttccaagat caatgcctgt tcgaattggt ctcgttgaat atttgtttcc
    13501 gtcagttttg gatgagggtc ttccatgtaa agttgcggac taacatcctg accgcctgca
    13561 agaatgagtt tgtcgatttt ctcaattagt tgaggcgctg aatctggaga catgactggc
    13621 agaataagag gtaatccttg agcatctaaa actgcatcca caaatccctg cggtgtatag
    13681 cttacttgat ttccttggaa agtatctgtc gctcgaatca gttgattccc ggcaatgccg
    13741 atgattggtt tcatttttat tccccctaaa gtagactaat tgtaacaagg atttaaaaaa
    13801 atttaaacta tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta
    13861 aagagaattt tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa
    13921 taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg
    13981 ggtgagaagg acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat
    14041 actttcaatg aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag
    14101 tgttagaagc tttggaacaa atccgaaaag cagaagaaga aaatgagaag cgtgctcgaa
    14161 ccctacggca gcagttgaag acctatgcag aagaaaaaga gcaagaattg gagataatcc
    14221 aaacgaacag tcggcaacag ctgaaacagt tggtagaaga aaaagaacaa ttcgaaaagc
    14281 aagaattaga aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt
    14341 tgaaagacca atatcaaaag catcagagtc aagcaatcgg tgcaatcatt gaaagggtga
    14401 aagaaactta tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat
    14461 caggagagga ttttacaagc agtacaagga atccaaaatg ttgaaatccg ggatctgtat
    14521 caaaatagaa caaacaacca gtgggtagaa aaatattttc cagataccac gattattgat
    14581 aaagaagcaa aattaagcgc tttaggcaat cggctgaaag acatccaaga agctatccaa
    14641 ttcatacaac accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt
    14701 tccttacaag acttggaagc aaattattcc gaagaaaatt tcatgaataa attagaagaa
    14761 gtccttgatt taaaagaaaa gtgggaacag ctaactgaaa aacgtgatcg tttagaagaa
    14821 gaggaagaat ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt
    14881 aagacgacca tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa
    14941 gaactaagta agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt
    15001 tatctttcgt atgttgcttt aaaaaaatca gttgctgcta tagaagaaat tgctgggaaa
    15061 tacgggttca ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa attgcaagaa
    15121 accaaacagc aattatccat tgttcacgag cagcaaaaaa agataacgac agcgttaggt
    15181 cagtgcagcg gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca
    15241 gaacgggaaa aagtaaaaga acggtttatc catgccactt atctgatcgt tttgcaaggt
    15301 tgggtagatg cagaagaaaa acagaatttg atgcatgtgt taaacgaaac tgtatcagaa
    15361 gaagatatat acgttagttt cgaaacacca aactcagaag agattcagca ggaagtccct
    15421 actaaattga aaaaccatcc gcttatcgaa ccttttgaat tgctgactga aatgtacagt
    15481 ctgccaaaat atgaagaagt agatccaacg ccttggatga caccatttta tcttgtcttc
    15541 ttcgggatga tggtcgcaga tgttggttac ggattgctga tgctcattgg gacaatacta
    15601 gctcaaaaac tatttgtttt accccgcggg atgaaacgtt tcgtaaaatt ctttgagatt
    15661 ctctcggtac catcgatcat ctggggattg atctacagtt catttttcgg acaggcatta
    15721 ccaaaagaac tatttggtat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc
    15781 aatacgattt tgattttatc cgtgattttc ggtctgatac agatcttggt tggattattc
    15841 gtggcagcaa aggaaaatat caggaggaaa gattacttga gtgctgtcag tgatggtttt
    15901 gcctggcaag ggatcctgat tgggatcgtt attgcattga ttggtgtcat gttgataaaa
    15961 aatacagcat ttgtttatct tggcgcgggg ctatcgatca ttgctgcttt atgtatctta
    16021 atcgtgccga ttatccaatc gccgtctaaa gtcaagggtg ctgccaaagg agcgtataat
    16081 ctttacggat tgactggtta tatcggtgat ttagtcagtt atacacgttt gatggcacta
    16141 ggtatttcag gcggaagtat tggcgcggca ttcaatatgc tagttgcttt catgccgcca
    16201 gccgctcgat tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt
    16261 ttgacactgt taagtgccta tgtccatggt gcacgattgc agtatgtcga attttttgga
    16321 aaattttata caggaggcgg acgtgccttt gacccgctga aaacggcgga aaaatacgtc
    16381 aacatcaatc ataagaaaaa aaacaataaa taattggagg atttttatac aatgatggat
    16441 tacttaatta gtcaaaatgg tggaatggta tttgcagtat tagcgatggc aacagcgacg
    16501 attttttcag ggattggttc agctaaaggt gttggaatga caggtgaggc agcagcagcg
    16561 ttaacaacaa gtcaacccga aaaattcgga caggctttga ttctgcaatt gcttccaggt
    16621 acgcaagggt tgtatggatt cgttatcgcc tttttgattt ttatcaactt aggaaatgat
    16681 atgtcagttg tacaaggatt gaactttcta ggcgcttctc tgccgattgc ttttacaggt
    16741 ttgttttcag gtatcgccca agggaaagtt gcagcagcag gtatccaaat tttagcgaaa
    16801 aaacctgaac atgctacaaa aggaatcatt tttgctgcga tggttgaaac atatgcgatt
    16861 ttaggtttcg ttatttcctt cctattagta ttgaatgcct aagcataaag gaggaaaagc
    16921 agatgaacgc cattgaaaaa atcatctctc agatgaatga agcagcagag caagaaagag
    16981 cggcgttaga gcaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc
    17041 aaatcgaaac agaacatcaa aaacaaaaag aaaaacaaat cgaattactt gaaaaaaaat
    17101 accgtcagct taggaatcgc caacaagtag aagtacgaca agaaaatttg aatgcgaagc
    17161 aggaattttt acgacgattg tttgcagatg caatagctga aatggagaat tgggatgaat
    17221 ctgaacaaat ccagtttatc aaaaattcat tgtattcatt acctttgaca ggcaaagtaa
    17281 cattcattgc tggagaaaaa tcagcagctt atctttctca gacattgctg gatgaatgga
    17341 acaatgaact tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt
    17401 ttctgataaa cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata
    17461 tccaaggaac gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg
    17521 tatggaatac cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa
    17581 tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa
    17641 atccacgatt tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct
    17701 tttaaaggag cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat
    17761 cgtttggatc tatacgatgc gctatacttt ccataatctg aaggttttga caaaagccga
    17821 gatgacaaat caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac
    17881 cttgaaggat gccattcata cgcaagtttc aaatgaactc cctgaaagca ttatggacag
    17941 tatacgagaa gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat
    18001 ttatgatcga cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga
    18061 attgctgaca gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg
    18121 aatattgcag aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat
    18181 cgaaaaagaa gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct
    18241 attagagaca gattatgctg attttctgcg ccctgtcatc cgtgagggaa atattgatct
    18301 cgtaaagctt gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac
    18361 ccaggctttc ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa
    18421 aaaccttcgt ttactggtta tcggcaaaag aagccatttc gaatcagaaa caatcagaaa
    18481 gcgggtgaga caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt
    18541 tgccattcaa attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc
    18601 aggcaattga agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg
    18661 cagcattgag cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat
    18721 tgattcccaa tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag
    18781 aaaaagcagt aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg
    18841 gaaaaatcat aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta
    18901 tccaagacat gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg
    18961 gcgatgtggc gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg
    19021 ttcgctctac tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt
    19081 ttgacggaat ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag
    19141 ggcgcggagt tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag
    19201 tagaagccgg agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag
    19261 ttataaaaca taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa
    19321 aaggttcatt cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa
    19381 atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa
    19441 acccggatgc acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa
    19501 aaggaggagc agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc
    19561 aaattgcaaa atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa
    19621 atgagatgac ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat
    19681 cgttgatgga acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag
    19741 aagcttctat ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg
    19801 ttgctatcat ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac
    19861 gtttggaaga aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg
    19921 aatactatga acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta
    19981 taaccgcaat cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa
    20041 atacattgcg ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac
    20101 attttccttc gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt
    20161 atatggatca tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt
    20221 tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag
    20281 ataatgaccg tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa
    20341 atgcttttga tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga
    20401 tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg
    20461 agatcatgga cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg
    20521 aaacagaatt agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga
    20581 tcgtttcaga aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc
    20641 ggtccattga tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc
    20701 cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca
    20761 atggtccaaa tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt
    20821 ttaggccatc ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt
    20881 ttgggacgac cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac
    20941 ggagaagtaa tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc
    21001 tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc
    21061 tctggtcttc ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat
    21121 tcatcagatg atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa
    21181 tttttcatgg aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat
    21241 ttagcaaacg accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca
    21301 gaatatcttg cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac
    21361 tatgcagagg cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga
    21421 tatcctgggt atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc
    21481 ggactaaaag gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact
    21541 catccaatcc ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag
    21601 ctttacaaga gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa
    21661 gataagggaa ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt
    21721 tttgcagcct atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct
    21781 gctttatctg atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat
    21841 gtcaatcaag gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa
    21901 ctgctatcga tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa
    21961 tatctgccag aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg
    22021 gaattgactc gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa
    22081 gataagcaag atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg
    22141 cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct
    22201 tcgatcacgg aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg
    22261 tctatcgtag acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat
    22321 gaacagctga cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat
    22381 cggtcaatcg atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa
    22441 aaaacttgtc aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta
    22501 gaatatatga cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa
    22561 gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac
    22621 taagtggtat ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga
    22681 aacgtttgcc ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg
    22741 gcggcctact taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg
    22801 cgcttttcac tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag
    22861 agcattggaa cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg
    22921 gttttatgat ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca
    22981 cacgtattat attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca
    23041 tgctttacat cctaaaattg gcctttgtga tccaattgat tggtacggtt gctttatctt
    23101 ttgtatttgt gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa
    23161 tctctagttt ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc
    23221 aaaatgttta tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca
    23281 tcgtatggcg tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa
    23341 tcgcattaac cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag
    23401 agcgcaatgg ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt
    23461 ttatgagtgt gacacctaga acggctggat attattcagt tgattacctc catatgagcc
    23521 atgcaggtct gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg
    23581 ccggcggatt gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga
    23641 aaggacgtac gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg
    23701 cacttacttt attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg
    23761 tgactgaaac gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg
    23821 cgtttggtac agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat
    23881 tgatcattat agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc
    23941 tgatgacaaa agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga
    24001 ttggataatt tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata
    24061 atttttcttg cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta
    24121 cccgttgttg aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat
    24181 tcctttattt atcaataggt tataattcat atttttttat tttttctttt acgatactta
    24241 gtccagaagc taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg
    24301 gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt
    24361 cattctcaat tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga
    24421 cacgattcgt tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg
    24481 taagacttaa tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga
    24541 tgacttccac atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct
    24601 tgctttggac atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt
    24661 ttgtcattcc agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga
    24721 tcgtatgttg atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac
    24781 cttctttttc aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta
    24841 taggattatt acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct
    24901 gaatcaatat atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat
    24961 gatataactg agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat
    25021 cacctggaga gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa
    25081 tagatagaga ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag
    25141 tcaattgaaa atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt
    25201 tattactagt tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa
    25261 ccagaccaaa atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa
    25321 agacattggg agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa
    25381 ttccatattt ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct
    25441 ctctttggga gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta
    25501 attgttgaat caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag
    25561 ttaacgggta attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac
    25621 catacaattt taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat
    25681 tgtctatgaa aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga
    25741 ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc
    25801 gattggtgga aattttacct gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt
    25861 atttggggga gaacagatat tttggcacaa aagtcattac cgcttttccg gataatcatc
    25921 agtatcattt accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac
    25981 ccaaagcatt gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc
    26041 tggcaacaga ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc
    26101 aacaagcatt gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg
    26161 tttacgatat tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc
    26221 ctaaaataac gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca
    26281 tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg
    26341 cacatatcaa tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga
    26401 tggaaacttc tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc
    26461 tgcttatagc catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg
    26521 aattagtttc tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg
    26581 cagttggtct agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga
    26641 aaggggcttt aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt
    26701 caaaggaaat ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca
    26761 aaaaattgcc attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga
    26821 atttgagctt agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt
    26881 agcaacagca tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc
    26941 tactcaaagc ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc
    27001 tagtattatg ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga ttggggctaa
    27061 gattaaagaa gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt
    27121 ggctaaattg cagccagatt ttacagctat caaaaatttt gaattaggaa taggagtcat
    27181 cgtgacagca gaaagtgtgc aaaaagactt tgtttctaga accttctttc caaaattaac
    27241 aatcaatgaa gatccagtat gtggatcagc acattctaat cttattcctt attgggcaga
    27301 aagaatgaat aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg gggatctaga
    27361 atgtgaatta aaagaagatc gtgtgataat aggaggaaaa gcagttttgt tttctaaagg
    27421 tacctcgttt atttgatttc tttcaaatga attaaaaata aattggtgga gagtggcaac
    27481 cttatagcta caatggttgt taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt
    27541 gcgtctcatt ttaattattg gtgtttgttt tttattcaat caagagtaaa atgccccata
    27601 ctaactatcc tcggatgact tcgattttat aaccatcagg gtcaatgaca aagtagtaag
    27661 aaggcgcagt gccaggtaat ccttttaagt cagtaatctc aagaccagca gctttatgtt
    27721 gctcgtgtaa tttttctaga tcatcagctg ctatagcgat atgaccatag ccgtctccta
    27781 gatcatacgc accatgatca tagttgtaag tcaactctaa ttcatagtca tccccaggaa
    27841 gtgtcaaata aacaagtgta aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa
    27901 aaccaaatgc cttttgataa aattcgattg atgcttcaag atctttcact cgaacacatg
    27961 tgtgagccat tttcatagat tcatctctcc ttttcctttt actcaatcat aacaaaaaga
    28021 agtaaggaaa tagttgattt gtgcttgttc atgagaaatt tctcatgtaa aatggataga
    28081 aaataaaact gttaaggtgg aaactattca tggataaacc aatttttggt gaaaaaaagc
    28141 aatttgctga ttatcaaacc cgttatgcag catatattgt catagaaaga gatcagcaga
    28201 ttgcagtaat cgaggctcca aatggtgctt tttttcttcc tggcggggaa attgaaggag
    28261 aagagacgaa agaagaagcg attgtccgtg agctgataga agaaatggga attgtggcag
    28321 aaatttcttc ttatctggga gaagctgatg agtattttta ctcgaattat cgtgcaactt
    28381 attattataa tccaggatat ttctttgttg ctgattcttg gaagcgtata ggagaaccaa
    28441 ctgaaaagac caataaaatt tggtgggtga gccctcaaga agcgcttgtg aaattgaaac
    28501 gtggtagcca ccgatgggca gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag
    28561 cgagtagatc agctttctct cagttttttt atttattaca gtacatattt tttgattgct
    28621 tcagctacac cgtgctcgtc gtttgatgaa gtgatgacat ttgctgcttc tttgacaaga
    28681 gaaacggcat ttcccattgc tactcctaga ccagcatatt caatcatgga taagtcgttc
    28741 tcgttatctc cgatagccat gatttcatct tgatcaatac caaggtgctg tgcgagattc
    28801 gcaactgccg cacctttact tgcttcttta tttagtactt cgaagtaata gggcgcactt
    28861 ttgaccgttg tgtatttatc acggaaagtt tgcggtaaac gcgcaattgc tgcatctaaa
    28921 atttctggtt cgtcgatcat catcattttg acgatgttca tgtctggtgt catttcttca
    28981 ggtgttcgat attttagcgg catcttgact aaacttgctt cgtggatcgt ataagcgctg
    29041 atatctcgat taggcgtgta gatcgctttg tcatcgatcg aatggagatg actgccgaca
    29101 cggcgtgcca ttacttcgat ctctaggaaa tcttcatgtg tcagtccgtg acgggcgatg
    29161 attttacctg atttcgtttg ttgtacgagt gccccgttat aagtaataac gtaatcatta
    29221 tcttgaaaaa ggttcaactc gtttaactgt tcctggacac ctggtaaggg acggccagtg
    29281 cataaaacga tcgaaacacc ttttgcaaca gcagcggcaa tcgcttcttt tacttctttt
    29341 gttacttctc ttttctcatt taataaagta ccatcgatgt cgatagcgac tagtttgata
    29401 gacataattt cctccaatat attttaattt atttcaatca atgcgccatt acggatatgg
    29461 ctggcaaatt cttgataagt ttcgtcaaat aaatcataat gatcacgcaa ggacggatca
    29521 accatttctt ttggaaagaa aaatcgctca tctccttgtt cttgtcccgc taatgcagca
    29581 acaagctgac taacctgtga taattctatc aaagttccgt catttcgcaa aatctcgatt
    29641 tgtgtgcgat gtctgccttg ttctgggcga taaaaatcat aaggtagatc gtagctagaa
    29701 ttcacagcag tgtaataaac aggattatat ccgacctttt ctactaacaa agtgagttct
    29761 tggactaatt ttgaatcccg gttgccagaa aaagtagcag atttcagtgg tttacgattc
    29821 aaaaaacgtt tagctaaatc atttaaaata ggatcagcag attcagacca ttgagtaaag
    29881 taagtgccca agaccccatc atcaagtttg aggtattctt ctaaagtaaa gcttccgtca
    29941 agaaatggca gaagtaattg agaatggagt tcaaaactgc tggcatcttc ttgatacaac
    30001 tcgtgtgcgc gatgaagcag atgctccaaa attacttcca taccacggga aacaggatgg
    30061 aagtacactt gcacatacat ttgatatcgg ctaacgatat aatcttccac tgcatgcatg
    30121 ccactcatgg aaaaagcgat cccgccttca taaggacgaa tcacacgtaa tattcgggta
    30181 agatcaaatg ttccatattc tgttccagtg aaataagcat cccgaagcaa atagtccatt
    30241 cgatctgcat cgatctgact agaaatcatt tggacaactt gtgggttggg gtaagttttt
    30301 tggatcacac tagctacctt ttcaggaaaa ccttcttcta ctcggttaag aatctgatac
    30361 acttctgttt ctggagaagt gatgatctct acagtgatgg cttcatgatt cgtatggaag
    30421 atatgttcaa atgtatgaga gtaaggacca tgaccgacat catgcaacag tgccgcacat
    30481 aaagtcacca gccgttcctg atcatcccag cctccgttgc ctattttttc ttttgaaaaa
    30541 tttcgagaaa agatatcgca gattcgtctt gaaatctcat aaacgcctaa tgaatgagta
    30601 aaacggctat gttctgctcc gtgaaaagtg aaagaagaag tgcctaattg tttaatgcgt
    30661 cgtagacgtt ggacttcttt cgaattgatc aaatctaaga ttacttgatg ctggacatgg
    30721 atatagttat ggactggatc acgaaaaact ttttccatag gcagcatttg atatttataa
    30781 ggggttgtca taatgtagct cctttatgtt attacgttgt tccattctct ggatttggtt
    30841 gttccattgc tccagctgga tttctttttt tagccagtcg gtagaatcct gtaaatcact
    30901 tgcagaatac agcttttcaa aagcttttgc aaatgcttct ttgacttctt ttacaaaaag
    30961 gggttggtca agcagttctt caagtgttgc cattgtagca ggatccactg caggatagcc
    31021 atttgtacca aagtcttctt ttaaagcaga ttgataaaat cgcttcatga cttgcccacg
    31081 tttttcctga ttaccgttga cacttaagta catcataaca gaaatcccat ttttgatccg
    31141 acgctgagcg ataccggcaa attttttgcc atggatgctt aaatcataag ttccaggaca
    31201 ataagaatgg gtgatttcga atgcttgtat cggtgccttt ggaaaagcgt atttagtcaa
    31261 taggagcatt tgctcgtaag cgtcatctat ggttattttt tttgtgtcac tttgggggaa
    31321 aatcaaagaa atattcaaaa ttccttcatc tgatacgatc cctaatccac ctgaatttct
    31381 gatcactgga gcgtaacctg catcttccag tgtttggatc ccgtcactta gaaaaggagt
    31441 tcgaacatcc ttcataccta agatgactgt gcagtcatat tgccagaaat gtaaaaagat
    31501 ttgctggtgt tctttagaat attccgttaa tatatctgtt aatgcaaaag gggaaaagtc
    31561 gtttttttga taaatgtgct gatctagtcg aatcgcaggg ttcattcaac cggctccttt
    31621 gtataaattc atattttcta caatattata ctctaaaatc aaagagttgt tttggaaaaa
    31681 aggttttctg taaaaatgga caagttggtt gacaaaacaa aagaaactag gaacaatgta
    31741 gctatgagaa gtaaaggaga atgaataatg gaattatcac aaggaacacc gatttcaatc
    31801 aaattgcgta caaaagtaaa acagcaaggg gaagtgcaag atttttattt tgatttgaaa
    31861 gggcaaatgg tgacaattgg cgatacgctg tacatacgtt ataaagagat tcaggaggag
    31921 acgggcgatg agattcctgt aacgatcaaa ttagttccag atggtcatgt acagctgatc
    31981 cgtgcaggag aaatgcggat gcgtttgaaa tttggctata aagaacggtt agaaacatcc
    32041 tatcgaacac catacggtat gatccagatt gctactttta cgaaagaact gcatgttagt
    32101 ttgaaagacc gtcccacagc agggaaggtt cgaatcgatt atgatctgtt catgggaccg
    32161 gaaaaaatcg gcgaatatta tttgaccttg gattttacag cgtaaatata aaagaaaagt
    32221 ttgatttttt gtgtctgatt ttgtatgata aggagtagac tgtgaaagga cgtgtcctga
    32281 ttggaaatta gtgtatttga aggtgcaaac aaaagcgaat tatcaatgat cgaagtagca
    32341 cacgcgatct tagaacaacg tggcgatgtc atggattttt ctgatttagc taaccagatc
    32401 caaaactatc ttgggaaatc agacagtgaa attcgtgatt cactcgcaca attttatact
    32461 gacttgaata ttgacggcag ctttatctca ttaggggata atcgttgggg cttgcgttca
    32521 tggtatgcta tcgattcgat cgatgaagaa gtaaaccatg gaatggacga agaggatgaa
    32581 gatactccac gtcgccgtaa acgcaagaaa gtcaacgcct ttatcaatga tgatgaagac
    32641 gcgatcgatt acaatgacga tgacccagaa gatacagatc tgacagaaga agatgacgat
    32701 gatctgtttg atgacgatga tgatgaagat gaagaaatcg ctgcttataa ttctgacttg
    32761 caagaaatcg gagcagatga cccgacagat gatgaagaag acttgccagg aaatatcgaa
    32821 gaagatttaa gtatcataga cgaagacgat gatgatgaag attatgaaga ggaagaattt
    32881 tctgactctg aaaaagaata aacattattt tcttacgaga ggttgtgaca gagacgatca
    32941 gcctatcaga ataaggaggt gtgacacgat ttttgtcaca ccttcttttt tgtatataaa
    33001 aatcccaagt aaatacataa aaaataaaaa aattgaaact tttagcgcac cttgtacgtc
    33061 atattaagtg aggcagaaag gagtggagaa tgatttttac tggatataag aaaaaacggc
    33121 agatcgaaaa ataccgtcac ctgctgaatg gactcatcga aactgattat caaaaaatgt
    33181 tccgtatcac gatgaattac gtacatgata aagaagaggc cttagatgtg atgcaagata
    33241 gttttcataa agccctgaac tattttgaaa aagaaaaaga gatcgagcat tttagtgcat
    33301 ggttttatcg gatattgatc cgcacggcat tagatagttg gcgcagacaa aaaaggacgc
    33361 ctgtggactt atttgaaatt caagatatcc cccaacagtt acctttagaa acttccgttg
    33421 cagagctcca tatgatttta gataaaatag acagtccgga aaaagagatt ctgatcctct
    33481 acttttttga aggatttcgt ttgaaagaaa ttgcgactat cttggatatg aatgaaaaca
    33541 cagttaaaac gaaaatgtac cgctcattga atgctttacg tcaaatccta gaatgaagga
    33601 gagaagagtg ccatgtattc ccaaaaagaa aatttaaaaa aactaaagaa aacttataaa
    33661 gaacaagcaa ttcccaaagg gcttaaagag gagatccgcc agcgttttat tatcgaagag
    33721 caatcattta taaaagcaaa gcggagaaag agacgtctcc aagggttcgg agtatctttg
    33781 gctatcgtaa tgattacaac aagtagcctt ctttttaata gtcaagttcg aagctttgca
    33841 gaagatttac ccatcttagg atcagtcatt gaattgatct taggcgaacg ctttactgat
    33901 cgatctgaaa aaatcgatat tcaggtaccg aagatcaata cacaaaatga gaaagaaaat
    33961 aaaacaattc atggattaaa tcaaaaatac tttcgcgaag gacaagcaga ttttgaaaaa
    34021 gctgaaaaag aatatggaaa ttttgaaacg gatcattacc aagttttggg agattatcag
    34081 aaaatcgtag acgataaccg gtttctcgtg attgaaaggc agatcaccca aacagcagca
    34141 gatagccatg ttgaaaaaag atacgacaca atcgacaaga aaaacagtgt ccagctctca
    34201 cttccacttt tattcaaaga tgacacctat ctatctgttt tgacaaaaga agtcaaacgg
    34261 cagatggccg aacaagtcaa ggaagatcca tctaagtact attggactga gcaagatatt
    34321 caagaaggga cgatagaaaa accaacactt gttacgccta cacgaagttt ttatttgaac
    34381 aaagagcatc aattagtttt gactttttct caatacgaaa ttgctcctgg ttatatgggt
    34441 acaccagaat ttgtcattcc taagtccgtg acaaaaacga ttttggcatc ggaagattat
    34501 ttggatcatt agacgagaag tagttgcgac tagcagtttc aattttcaag taagtacaag
    34561 taaataataa gaagagaaaa agagaaagaa gagatatagt gaaaagaaat tatttagtta
    34621 tcagtattat tgctttatcc gtgcttttgt ttttcgaaat tacttcggtg gcctccttga
    34681 tcaccgaagt aaaaggacaa acacactctt cggagcattc gtcacaatct ttcaaacaat
    34741 cagaacaatc aaccattaaa agccaatcaa ctgaaacagc taacgtcaaa gaagccattc
    34801 aagaatcagc ggaaaaggta caaagtcagt catcacaatc aacacaagac agtacaaaaa
    34861 cgagccgaga tccggctgtc aaaagcgtcg caatcagctt tgatgatgga ccaggagcga
    34921 ccacaacacc gcagcttctg cgaatcttaa aagaaaaaaa cgtgcatgcc acttttttcg
    34981 tcttaggaga aaatacggcg cagcatcctg aaatcgtgaa gcagacagct gaagcagggc
    35041 acgagattgg caatcacaca tacgatcatc agaatctggc cactctttct gcccagtcga
    35101 tgacggagga agtgacgaaa gcagacactg agatcaaaaa agtcactggc aaaaccccca
    35161 cttttgttcg tccgccctat ggttctgtca cgaatgtagg agcaacgatc atccagcgac
    35221 cgatcatcga atggtctgtt gattcagaag attggaagac aagaaatcct gatttgattt
    35281 tgcagaagat ccaagcaaca gtatacgatg gggcaattat tttattccat gatatctatc
    35341 ctgaaacgat tcgtgccgtt cctcaagtga tcgattatct gaaagagcaa gggtatcgca
    35401 tcacgacagt tggggattta ttagggcatc caacggctgt cgagaactat tatggcagaa
    35461 acgatcatcg gccagttcag taagtgagac aagtatcttt aataaataac acattttaat
    35521 aagtagttga acacataact accttacttg caaaggctta acaatatcta tacaggtaca
    35581 acaaaaaact gtccaccaat tt
//
LOCUS       DAXWLF010000009        34013 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXWLF010000009 DAXWLF010000000
VERSION     DAXWLF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.009"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(57..254)
                     /locus_tag="ACGE4U_000319"
     CDS             complement(57..254)
                     /locus_tag="ACGE4U_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392511.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            423..1001
                     /locus_tag="ACGE4U_000320"
     CDS             423..1001
                     /locus_tag="ACGE4U_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFF8392512.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            1096..1233
                     /locus_tag="ACGE4U_000321"
     CDS             1096..1233
                     /locus_tag="ACGE4U_000321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392513.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(2046..2549)
                     /locus_tag="ACGE4U_000322"
     CDS             complement(2046..2549)
                     /locus_tag="ACGE4U_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFF8392514.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            2908..3933
                     /locus_tag="ACGE4U_000323"
     CDS             2908..3933
                     /locus_tag="ACGE4U_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8392515.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            4256..5135
                     /locus_tag="ACGE4U_000324"
                     /pseudo
     CDS             4256..5135
                     /locus_tag="ACGE4U_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            5611..5727
                     /locus_tag="ACGE4U_000325"
     CDS             5611..5727
                     /locus_tag="ACGE4U_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFF8392516.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            6655..6993
                     /locus_tag="ACGE4U_000326"
     CDS             6655..6993
                     /locus_tag="ACGE4U_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392517.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            7087..7317
                     /locus_tag="ACGE4U_000327"
     CDS             7087..7317
                     /locus_tag="ACGE4U_000327"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFF8392518.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            7717..8088
                     /locus_tag="ACGE4U_000328"
     CDS             7717..8088
                     /locus_tag="ACGE4U_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFF8392519.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(8362..9054)
                     /locus_tag="ACGE4U_000329"
     CDS             complement(8362..9054)
                     /locus_tag="ACGE4U_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8392520.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9057..9842)
                     /locus_tag="ACGE4U_000330"
     CDS             complement(9057..9842)
                     /locus_tag="ACGE4U_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8392521.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            10079..11479
                     /gene="lacG"
                     /locus_tag="ACGE4U_000331"
     CDS             10079..11479
                     /gene="lacG"
                     /locus_tag="ACGE4U_000331"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFF8392522.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            11504..11821
                     /locus_tag="ACGE4U_000332"
     CDS             11504..11821
                     /locus_tag="ACGE4U_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8392523.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            11834..13507
                     /locus_tag="ACGE4U_000333"
     CDS             11834..13507
                     /locus_tag="ACGE4U_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFF8392524.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            13521..15152
                     /locus_tag="ACGE4U_000334"
     CDS             13521..15152
                     /locus_tag="ACGE4U_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFF8392525.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(15643..17208)
                     /gene="guaA"
                     /locus_tag="ACGE4U_000335"
     CDS             complement(15643..17208)
                     /gene="guaA"
                     /locus_tag="ACGE4U_000335"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFF8392526.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(17267..18190)
                     /gene="coaA"
                     /locus_tag="ACGE4U_000336"
     CDS             complement(17267..18190)
                     /gene="coaA"
                     /locus_tag="ACGE4U_000336"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFF8392527.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            18386..18991
                     /locus_tag="ACGE4U_000337"
     CDS             18386..18991
                     /locus_tag="ACGE4U_000337"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8392528.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            19299..20315
                     /locus_tag="ACGE4U_000338"
     CDS             19299..20315
                     /locus_tag="ACGE4U_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8392529.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            20456..21757
                     /locus_tag="ACGE4U_000339"
     CDS             20456..21757
                     /locus_tag="ACGE4U_000339"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFF8392530.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            21803..22465
                     /gene="deoC"
                     /locus_tag="ACGE4U_000340"
     CDS             21803..22465
                     /gene="deoC"
                     /locus_tag="ACGE4U_000340"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFF8392531.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            22486..22884
                     /locus_tag="ACGE4U_000341"
     CDS             22486..22884
                     /locus_tag="ACGE4U_000341"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFF8392532.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            22950..24032
                     /locus_tag="ACGE4U_000342"
     CDS             22950..24032
                     /locus_tag="ACGE4U_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8392533.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            24244..25806
                     /locus_tag="ACGE4U_000343"
     CDS             24244..25806
                     /locus_tag="ACGE4U_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8392534.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            25799..26932
                     /locus_tag="ACGE4U_000344"
     CDS             25799..26932
                     /locus_tag="ACGE4U_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8392535.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            26932..27888
                     /locus_tag="ACGE4U_000345"
     CDS             26932..27888
                     /locus_tag="ACGE4U_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8392536.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            27987..29153
                     /gene="deoB"
                     /locus_tag="ACGE4U_000346"
     CDS             27987..29153
                     /gene="deoB"
                     /locus_tag="ACGE4U_000346"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFF8392537.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            29177..29995
                     /locus_tag="ACGE4U_000347"
     CDS             29177..29995
                     /locus_tag="ACGE4U_000347"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8392538.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            30012..30719
                     /gene="deoD"
                     /locus_tag="ACGE4U_000348"
     CDS             30012..30719
                     /gene="deoD"
                     /locus_tag="ACGE4U_000348"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8392539.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(31017..31703)
                     /gene="gpmA"
                     /locus_tag="ACGE4U_000349"
     CDS             complement(31017..31703)
                     /gene="gpmA"
                     /locus_tag="ACGE4U_000349"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFF8392540.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(31875..32456)
                     /locus_tag="ACGE4U_000350"
     CDS             complement(31875..32456)
                     /locus_tag="ACGE4U_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8392541.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(32589..33272)
                     /gene="rpiA"
                     /locus_tag="ACGE4U_000351"
     CDS             complement(32589..33272)
                     /gene="rpiA"
                     /locus_tag="ACGE4U_000351"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFF8392542.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            33591..34004
                     /gene="rpsL"
                     /locus_tag="ACGE4U_000352"
     CDS             33591..34004
                     /gene="rpsL"
                     /locus_tag="ACGE4U_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFF8392543.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
BASE COUNT        10641 a         5948 c         7181 g        10243 t
ORIGIN      
        1 tcgcgaccct atttttttag taagttatat cataatgaag tgaatggtat catacattaa
       61 taatagtttt ttctatgtgt atttcctttg atccagcgaa caagccagat gataagtgcg
      121 attgggatta gaactttgac cgcaaaccat aaaagcccgc ttaggacact gacaaaaaga
      181 ctgatgacga agctagctgc aattagcact aaaagaacca tgaagatatt taagaacata
      241 tttttttgac gcatacaaaa ccctcctctt gttcattctt tactatagaa aaagtataac
      301 agggattgtt taaaaacacc tccaactgaa gactgatttt tacttcatct acttaaatga
      361 tcaaacaatt gctttttctc tggtttatca tctaaaatag agcggaaaag gaggagaata
      421 tcatgcctaa aaatcgtctt gaagcattta cagatgctgt aatcgcgatt atcatgacta
      481 ttttagtttt agaacttcat gaacctaacg gagacacgtg ggaggcgctc gctgcattag
      541 gttaccgatt ttttatctat attgtcagtt ttatcatttt agcgatttat tggaacaatc
      601 atcatcattt atttcaagtt gtacataaga taaatggacg ggtcttatgg gcgaacagtt
      661 tctttgtttt tgcgctttcg ctttttccgt ttgcaacttc atggatcagt gagtacatca
      721 atagcctggc tccagaaatg acttatggtt ttgttacctt gaccgctaat attgcgtatt
      781 atatcttggc aaaagaactt gtcaaagcaa ataaaggcca cgagaaagca ttgaaggcga
      841 tgttgaataa ttacaagaaa tcgtatctat cgattggttt gaatgtgtta ggaattgtct
      901 tagggtattt agtagcgcca gtagccgtgc tgatcgcaaa catcgtgatt ctagctgctt
      961 ggatcatccc aagtcgtacc atcgaatcac aatatcggta aaatgatccg tgtttaaaat
     1021 ttaagagaaa aatatagaaa aaataaataa acgatcaaat atccgagtga tactagtatt
     1081 caaagaggga tgcagatgga tcaaaaaaga attcatgtag gactgattac tagttttgtc
     1141 acgatgctta tttatttgga tcaaggtcat ttgacttgga cagtagttgg tgtcgtagga
     1201 tttttgacat tattcacagc aataaaaaaa taaacatctt tatgtaatct gatgttctat
     1261 ctatttaaga aaagcatgtt tacagcagta tgattctgtt gtaaacgtgt ttttatttgt
     1321 ggaaaattta atttttagaa aagtgtatcg aaaaatagat agttagtttt tccacagtgg
     1381 ataacttcgt aagagataaa aataaaaaat tagtcaaaga agggcgtata gatttctttt
     1441 gataggagat atacaacaga aatgaataaa tattaacgtg ttttcaaaca gtataaaacg
     1501 gtgtggtttt tggaataaaa aaataacata tctcatttta cgcttttgtt attttatact
     1561 taaaaaataa cgacacagaa aataccgtgt ctcagatttc tctactactt ctaaaaacta
     1621 ctgttttttt cctttgaaat gccttcaaaa agtaggatca ggcagaatat actggcagaa
     1681 tcaaggagaa gggagcctaa ataaggagaa aaaccataga aaatataaag taattactta
     1741 taaaacaagc tttttataga aaaataatat acctgagtag ggtataaatt aagacggaat
     1801 tcctttctag attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa
     1861 aaaaaaaccc tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg
     1921 tcctgctgca ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg
     1981 aataaaaaga ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa
     2041 cgattttatt gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag
     2101 aataaaatgt atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg
     2161 tttagaagtt gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca
     2221 gtgatgtccg ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca
     2281 aggaaaataa tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc
     2341 accccaaaag gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt
     2401 tcaaaagaaa aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat
     2461 tgtggaagat taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc
     2521 cctaataaaa attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat
     2581 cgaaaaccgc tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta
     2641 cgaaaattag aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg
     2701 agttgcttgg aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt
     2761 gttctaattt ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata
     2821 gttgttatac taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt
     2881 ttccaattac taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata
     2941 caagataatc cttttcgtat catgaatctg atgctactta tctttattgt attatggtta
     3001 ggttacataa atcatgattt tttgatttca agtttttcat cattagggat ctttactttt
     3061 ttctattatc aaaacatacc gatgaaatat ttgctgaaac gtatgtttct gatcggttgc
     3121 ggactgttgg ttgctttcgt tttggggatc ttatcgactt atgttttatg gcttgaacca
     3181 tttgccgtag ctttggttgc tttttccagc agattcgtgt tgcgattatt ccatatttct
     3241 aaaccaggtg gcttgttttt tgcgatgctt tctgccatgg gaacgagcat gcagctgcca
     3301 atcgctcaat taccgatcgt ctcactttat ttttttatgg gtgtcgtttt tgccctgatt
     3361 gcggcagtga ttacaaaatt actggattca cggcctgagc agacaattga aaaagcaact
     3421 ttaaaagaaa gatttcacga agagccttta gtgatcattg attctgtttt ctacagcgct
     3481 gccttatttc tttccgttta tgtcagtcac ggcttgaatc tgcataatcc ttattggttg
     3541 accttgtcat gtgcatcgat tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa
     3601 gtccaatatc tgattggatc gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg
     3661 ccgtttacgc agttgcagac catttttctg atcacttttc tatatggaat cgcacaattt
     3721 ctagttgcta gaaattatgc agttgctaat atttttctta atccgatggc gttgatgcta
     3781 tctacactta ttcgtggagc atacttgatt tctttgatcg aatatcgttt tttagggatc
     3841 gtcataggaa gtttcatcgg tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat
     3901 tatttaacgg ttgttcgtca aaaattagaa tagaaggaca aatatatgtg ccaaaagagg
     3961 ttatgacaag atttttgtca caacctctaa ttcaaggtgt tcaaaagcca gattctcagc
     4021 attaagtcaa attcacacaa aaatgtggaa aggaactttc aaaaaaattt tatatttatt
     4081 ctatcaaaaa attttgttta tgatggtaac tgaataaact ttcctgagat ggtcaatgct
     4141 ttccttgaaa gtcttacaga tattctttct tcatagaaag tttaagtttt atagttagtg
     4201 tacaataaaa gtatgtttat accatatggt gtccggataa tcagtcattt aaacaatgag
     4261 gttcaaagat atgaaaaggg gaaatttgat gaagaaaata agcaatgaaa tccaaattct
     4321 ggcagaaact ttaactcttc ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa
     4381 catccttgat gaggtgtttg agcaatatag tcatgaacta atggatccta ataaagccaa
     4441 aggcgactgc aaagaaaata gacaaagtct atgaagagga accttttgca gcattagcta
     4501 tttatctata tgcagcttct caaagttgga aaaaaattta tcaaccattg aagattccaa
     4561 gaactatcta tttggcaaca atgaatgctt ttactagatt tattcatgaa catttccaag
     4621 taacaggaag ctatcgcttt gaccgtggtt tttggatttg gcattacact agtggcttga
     4681 tctttcggat tggtgaattg gagtacgaaa tgacatattt tccgaaaggg tacacggatc
     4741 taagattaga aggaaagcag tgcctttctc ttcatattcc ttccgatgca gatttatctc
     4801 agaataagat aagagatagt tacgaaagtg ccattcgttt ttttgaactt tattttcctg
     4861 attaccatta ccaagtgatg tacacagata cttggttact atcacccaat cttactaaat
     4921 ggttgaaaaa agagtcaaaa atctgtcttt ttgcagccga ttatcgatta ttatcagttg
     4981 atgaacagga cgatagtgga gtcccttgga tctttggacg agtagatgcc caaatacatg
     5041 attatccaga aagtacgagt ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc
     5101 acattggctc tggcttaggt attcttactt cataagttta gtacttcgaa atagtttaaa
     5161 agagcgtgag acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
     5221 ggaagcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt
     5281 ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttagt
     5341 gttctatcga acacacagta cgttcaatag gtaaactagc tataacaata gatattaaag
     5401 ttaacggaca agagggcctt ttttaggtag ctaaaacgta ataatgaaaa tttatttcca
     5461 gagataggag tcaatcagct agtctgatcg actgttttaa caagttgact ttcattttaa
     5521 cttttgatac catttaaaac acaaatcgta attgtttcga tttgtgtttt ttagcatgac
     5581 tacaatcaaa gaagaaagtg ggtcgtccat atggaaaaaa cagatatctc aagtgcatac
     5641 cgccgcttga aaagcccaaa tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat
     5701 gtgaaacgga atagtgggaa aaggtaagaa gaaggaaact taattaaaaa aatagccgaa
     5761 aaacgttttt cggctatttt caataatcgg tttttgtgta aggtttccga aagaactgct
     5821 gttgattttc catttttaac atatgataaa ttggtagaat ctttcttttc aaattgatac
     5881 cattccctaa ttccagttat atagttaact acagaaccac aaagcacgta cgtttataaa
     5941 agtattatct gtagttattt ttcaattccc tttcaacctc ttattttgac cagtagtatt
     6001 gatcacttta ccttttaaca cctttgttcg tttttgaaac atttttagtc aagaatggaa
     6061 acgtaactac ataatcaaag gttttgcgaa atatttcatg aaatatttca tgtagaagcg
     6121 agcaatacgg ttgcaaataa ccattatcgt ttaataagca gttacgaaag ctcccaatat
     6181 ctctaacgtt cagctcactc catttctttt cttcaaaaaa atcagtgatt cgtatctctt
     6241 tctatttgtc gaaaaagtta actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa
     6301 aaaagcagtt actattgcta gaataacatc aaggaataat aagttttggt tttcagataa
     6361 tagtcgataa gttgtgtatt ggaattttag atacagtaat aaagtatttt tcatgaaatt
     6421 ttgtgagaat cactattaca agtatcttta ataaaattaa tggttaagga ggaacttaaa
     6481 gtagaatgta aatgaatagt tgtagtcagt cgattgtgat actagactca aagttgcagg
     6541 aataagtttt tcaatttgtg attggaagat aaaaaatgaa aaagaagggc ttttttttat
     6601 actttgttga ttgtagtgtt tattttccct ctgtttagcc aagtaacatt ttctatgact
     6661 caaccagttt caagtgagga acatgataac ctttttcgtg ctacagaaga gtttaagaaa
     6721 tatcaggaaa atattgttcc ggagactcca ttagctagtg agccaattac tgatattgat
     6781 ggaaaccaaa taggatggta tatacagtac gaagttatgg tggtaaataa tgactcaata
     6841 gtaaaaaata cagtaggctt agagctaaca ccgattgaaa aggtgagttc attgttaacg
     6901 tatatttata ctgatgaaag tgacgagact gagatcattt taacggatta ctcaaaaatt
     6961 gctactgata aaaagtatat attgtggatg tagacactag tgaaatactt tatgatatag
     7021 aaattgatga aaatttacta gatgtagcac aagaaatcga tcatcaaaag caggaagcta
     7081 ttcgatatgc tggtagaaaa gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt
     7141 agagaagatg aaaatgagtg gaaagaattc aagagtcgct actcaattaa ctcgactccg
     7201 acatttacag tttacagaga aggatcgata gaaaaaactg ttttttggac taaagaaagt
     7261 ggaatgtctt tagctgaagt agaagagttt ttggattatg ttagtatgca acagtaaggg
     7321 agttcttatt atgaaaaatt ttgacacata ttatgttatt gttttttcga tatctttgct
     7381 gttaatatta ctaactttaa tgcagtataa agagtatcca gagtatttta ttttaacttt
     7441 tgtatattta gtaagtagct acacacttct atataaattc agaagttctt ctagagtttc
     7501 taaagtttgt ttcttaatta gcttattgat tctataatat ttttgattta tataggaaat
     7561 taaaaatatt atagaatcaa cactcagaga acgggttggt ttttaataga tttaataagt
     7621 actgataatg aaatttctcc tggttgggaa aacgaagttg gttaccctgt agaagaattt
     7681 tttcaagatt actttgcgga gtatgccgat aggataatga gagattacgt aacagataaa
     7741 actcaagaag tcatcaatca atcccagtca ttaaatgaat tatctgataa ggcgaaagaa
     7801 gaagcagttg ccattatttc ttcagggatt agtgactata ttgatatgag tggtgaaatc
     7861 aattatggcg ataaagacaa aattgtacgc atgataaaag cttttatcaa aacttttgat
     7921 gtgaaggcag atcttaagtt taatgatact tacctagtag gaaaattaat taatatcctt
     7981 gggacagaga aagatacgga aacgtttatt tcctctttgt ctatgttgtt tacagataag
     8041 catttttcaa ctcagcttgg tggagaggaa ctagtggaaa tcttttaacc attacgccca
     8101 gcattaataa aagtatatca aaaaagtact cctgatgatt tttaagattg gtttgagaaa
     8161 taatgtgaaa acactatttt taatgttaat tatagataga gatgaacgcg tcatatggtt
     8221 aaaagaatga aaaagtcaaa gatacactgc tatatttcac taaggatata gcagtgtatc
     8281 tttcatgtcg tttcactggc agttttttat aaaaggaggt gtgcatttca gttgcttttc
     8341 tttggtagtt agttgaatcg tttaagaatt aacggatgtg gcagacaggt gctctgtcca
     8401 aaaaccagta aaatctttga tggttccatc tttatttagc tgtgcctgtt caaatactgt
     8461 tttgttttta ccgaatggat caattgcaat aattgttccg atccccgcac gtttggcagc
     8521 aagtaatcct gagtaagcat cttcgataac aagacaatca gccggagcta agctcagttt
     8581 gtctgccgct ttaagaaaaa tatccggttg cggtttccca gggaaagagc cgtcgtcata
     8641 gacaatcttg tcccagtcaa accagcgggc taaatcgaaa atatcaaaat aaaaagcaac
     8701 attctctttg acagtagccg tggcaatcgt taatggaatc tgttggtcag tcagctggtc
     8761 aagagtcgct tcaagccctt ttgtcaactg aagctcgttt tcattttgca agcaaagcat
     8821 acgataatag ctttcttttt cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc
     8881 tgaaatgaaa tgtttcaaaa tctccgtatt cgtccgacca tgaatatttt ttaaaatatc
     8941 ctcgtctgat aaaccatctt ttgtatgatc atgtatcata tgcagccaag cagcttcatg
     9001 cagatgagag tccaaaaaca ttgttccgtt gaaatcaaat ataatccctt tcatagttac
     9061 gctcctatag tggtattttc ggttgaatca gcaatttgca gatcgacata ttgtgcatag
     9121 tgctcttgaa cctcccgcga gatctggcga tccgtaatga gcagatcgaa atcatgcaaa
     9181 ttataaaaca cataaaaatc atctcgattg aattttttat tatcaacgag tagatatttc
     9241 ttacgggaat tatttagagc gatctgctgt gcttctcctt cttcggtact gtaagtagag
     9301 atcgcttcgt tgtgaacgcc atttgaactg ataaatgctt ttgtaaactt tagtcttctt
     9361 aaattgtcgt tggtgatcgg tccgacaaat gcgccggtat ttttccggaa atcaccgcca
     9421 atcagtagaa tttctggtcc atctgcttga gcaagaatat caaaaactgg ataattattt
     9481 gtaatgatcc gaattttctt gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct
     9541 ggtcctaaga aaatcgtttc tccctcgtga atcaaggagg ctgctgtggc agcaatttct
     9601 tttttttctt gaatctgcag agtggctttt tcagtgtgag aaagttcttg atctaaagtg
     9661 taggaaagac tttgagcccc tccatggaca cgcagcagct tgcccgattt ttctaattca
     9721 tctaaatctc tgcgaacggt catatcagaa acatctaatt gattgatgat ttcattaacg
     9781 gtaaggattc cttttttatt gaccatttct acgatagtaa gcaatctttc ttttttcaac
     9841 attgtcttca cctctaattt aaggataatc tatcatataa acggacaaca gtcaatctaa
     9901 ttcgaacaaa agtaaacatt tagggtggtt tttatgtttg ttacaataga ttttattgct
     9961 gttttctggt ttttatgatt atttgtgttt gtttttgttg acaaatgttt tggatgcgtt
    10021 tacaatgcaa tcagttagaa agagcgaaaa ctgattatag atttgaggag agatgtacat
    10081 gcttaaatta ccagaagatt ttatttttgg cggagccaca gcagcctatc aggttgaagg
    10141 agccactaag gaaggtggca aaggcgctgt tgcttgggat gactttttag aggaacaagg
    10201 gcgattcagt cccgatccgg caagtgattt ttatcatcaa tatgcaaagg acattgaatt
    10261 gtgtgaacgt tttggtgtaa atggtctaag attatccatt gcttggagcc gcatttttcc
    10321 tgatggagct gggaaaccaa atccagaagg aatcgctttt tatcatcgtg tgttcgaaga
    10381 atgtaagaaa agaaacgtaa cgccgtttgt gacgttgcat cattttgata cccctaaacg
    10441 tttgtttgac catggagatt ttttgaatcg agaaacaata gaagcatttg tttcctatgc
    10501 cattttttgt tttcatgaat tcaaagaagt caaggtgtgg agtaccttta atgaaatcta
    10561 tccagttgca accaatcaat atttgttagg tgttttccca ccaggaatca aatatgattt
    10621 tacaaagatc gtcgcctgtc ttcataatat gatggttgct catgcccgtg tagtgaacta
    10681 tttcaaagaa aacgaacttc cgggtgaaat tggtgtagtt cactcattag agacgaaata
    10741 tgcggcaaca gacgcgccgg aagataagca tgctgccttt ttagacgatg cattgtcgat
    10801 ccgtttctta ttagatgcta cctatttagg ctattattca actgaaacct tgactgctct
    10861 tgatgagata tgtgaagcaa atcaggccag ctatcatttt cctgaagaag atttcgttga
    10921 attgaaaaaa gcatccacgc gtaatgacta cttagggatc aatcactacc aatgccattt
    10981 tgtcaaagct tacgatggag aaaatgcaat ccatcataac ggtacgggtg aaaaagggac
    11041 ctctgtttat aaagtgaagg gaatcggaga acgtatttat aaagaaggaa ttcctcgaac
    11101 tgattgggac tggctgattt atccagaagg tctatatgac ttgttgctgc gaattaaaag
    11161 cgattaccca cattacaaca aaatctatat cactgagaac gggatggggt ataaagatca
    11221 gtttgaagat ggcattatta tggatcagcc gagaatcgac tatttgagag tctatttgga
    11281 atccttaagc aaggcgataa cagccggggt aaatgtgaag gggtattttt tgtggtcgct
    11341 tatggatctt ttctcatgga caaatgggta taacaaaagg tatggcttgt tttatgttga
    11401 ttttgaaacc cagaagcgtt acccaaaaga atcagcttac tggtacaagt tggtcagtga
    11461 aaccaaaacg attatttaat aagtgataat ggaggaagaa aagatgaata aagaggaatt
    11521 acaaatgctt ggttttgaga tcgttgcata ttcaggcgac gctcgcagta cattattgaa
    11581 attattgaaa gaagtaagac aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc
    11641 cgacgaaaat ttaacccttg cacataattc gcaaacaaaa attttagcgg aagaagcatc
    11701 tgggaaagag atggagatgg gattcatttt tatccatggg caggatcatt taatgaccac
    11761 tttattgctg agagatctga ttcaagactt tattgtactg tacagacaga atcaaggata
    11821 ggagaagaac gctatggatg gaatcattaa acaaatcgaa aaagggaaac cattttttga
    11881 aaaaatctca cgtaatagtt atttaggagc cgttcgtgac ggatttttaa cagcgatgcc
    11941 agctatttta ttttcaagca ttttcatttt gattgctgcg gttcctgaga tttttggtta
    12001 ccaatggcct gaatctgtct ccacatggct atggaaagtc tataattatt cgatgggtgt
    12061 tgtagggtta ctcgtttcag caaccactgc tcgctgcttg gctgattcga tgaatcgtcg
    12121 cttaccgaaa aacaaagtaa tcaacaccac ctctgtgatg ctggcctctg tggtaagttt
    12181 cctgttgtta agcgtctctc cgattgaagg tggattttca acggaatata tggggacaaa
    12241 gggactactg gcttcctttg tcgctgcatt tttaacagtt aatttatata agttttgtgt
    12301 gctgaaagat attaccatta aaatgcctaa agaagttcct ggaacgattt cgcaaacctt
    12361 ccgagatatt tttcctttct ctttttcagt atttgcggcc gttttaattg atttaggcat
    12421 tcgacaaatc ttccaaatct catttgcaga agcaatcatt acggtgctgc agccgctatt
    12481 tacagcagct gacgggtact taggaatcgc tattatctgg ggagcgatgg cgctgttttg
    12541 gtttgtcggt gttcatggac cgtcgatcgt ggagccggca attgcggcaa ttatctatgc
    12601 caatgtggaa accaatctgc aattgttcaa ttccggtgaa caagcttcta aagtactgac
    12661 ggtgggttta gggaattttg taggaactat gggaggaact ggggcaacat tggttgttcc
    12721 ttttatcttc ctactctttg ctaagtctaa gcagttgaag gcagttggga aagcttcctt
    12781 tattccagta agtttcgctg tcaacgaacc attgctattt gcggcaccca tgattttgaa
    12841 cccttatttt tttattccat ttttgttaac acctgttgta aacgtttgcc tgttcaagtt
    12901 ttttgtcgat gtattgggca tgaacagctt tatgtacgtt ttgccttggg caactccagc
    12961 accgatcggt ttggttctag gaacgggcat gggtatctta gcttttgttc tagcagcggt
    13021 tctaatcgtc gttgacttcc tgatctatct accattttgc aaagcatacg atgctgtctt
    13081 agtagagaaa gaagcacaaa aagcagaggt gatcgcaacc gaggaaggtg cttctgccga
    13141 tgtacgtgag gaagtacctg tggcagcgag tcaagttatc agcgatgcaa ataaagaaat
    13201 caatgtgttg gtactttgtg ctggcgcagg gacaagtgcg atgttagcta atgctttaaa
    13261 aaaaggtgca tctgaatcga aagcaccgat cagtgcaatg gcgggtgctt atggttctca
    13321 ttacgaaatg ctgcaggatt ttgatatggt ggtcttagca ccgcaagtca attcttacta
    13381 tgatgatatc aaagaagaca ccgatcgctt aggaatcaag ctggttgcga caaaaggagc
    13441 agaatatatc ggattaacga gagatcctga aaaagctgtt cagtttattc tgaaccaatt
    13501 tcaataaagg aggagccata atgaaaatgg gaatttggtt gcgtaaagct gttttatttg
    13561 cagctgtgtt aggggctttg ggggctgttt caaaaactgt tgaagcatct gccagcgaat
    13621 ttattcgcgg agcagatatt tcgatcctgt cagatatgga aaaatctggt gcaaaatatt
    13681 atgaagacga tatttcaaaa gatgccttag agattttaaa aaataatggt gtcaactatg
    13741 ttcgactgcg tttgtggcag gatccttata atagtaatgg agaaagttat ggagcaggga
    13801 caaatgatct ggaaacgacg attgaccttg caaaacgagc aaaaaatctg ggacttaagg
    13861 tgctgctgga ttttcactac agtgattttt gggttgatcc tgggaaacag aatcttccta
    13921 aagcatggca aggtctgacc tttgaagaaa tgaatacggc attgtacgac tatacaaaga
    13981 acgttctgtc ggaaatgaag cagctggacg tttatcctga tatggttcag atcggcaatg
    14041 aactgaatag cggaatgctc tggccttatg gcaagtcttg gggagaaggc gggggagaat
    14101 ttgatcgatt ggctgctttt ttgaagtctg gtatccaagc tgttcgagac actcaaccta
    14161 aaacaactcc agtaatgctc cacttagctg atggtggcga tacaggagca tttacttggt
    14221 ggtttgatga aatcacgagt cgaggtgtgt catttgattt aattggagtg tcgtattatc
    14281 cgtattggca cggttcaatg aatgaacttc aaaccaatat gaatgcgatc agccaacggt
    14341 atggtaaaga agtgatcgta gttgaaacag cttatgggca tacaacagcg aatgccgata
    14401 ctatgccaaa tgcttttggt gaagccgaag cagctgcggg gggatatgaa ccaacaccga
    14461 caggacaagc agagtatttg ctggatcttg ccgatcggat tcaggcagta ccgaataatc
    14521 gcggggcggg cttcttctat tgggaacctc tttggtacaa cggaaatgtg agttgggcaa
    14581 cacaagcagg tatgaattat ctcagtgttc aatccactat gggaaacgaa tgggacaacc
    14641 aagcgatgtt tgattttgct ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag
    14701 gggcgcaaat aaatctagtt aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc
    14761 cttccaattg gcaaacctgg tttcaacaag gaaccgctaa gggtacgatc aaaacggaag
    14821 cgggcacttt ttttggggac tacaaactaa ctttttggca ggatcaagca tacgaggcat
    14881 cagcttatca aaatgttagt ggcataagca aaggaactta cacattgagt gcctatgtaa
    14941 tgtctggggg agagtttgat actaatcagt tatatatcaa aaattatggt ggagcggaac
    15001 taaatactgc ggttgttaaa acaagtgaat ggacaaaaat tacaatcaaa gacattccaa
    15061 ttacaacagg tgtttgtgaa attggggttt atagcaaggc ttcaggaaat gcttggacga
    15121 acgttgattt tgtttcattg gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg
    15181 aggttccctt cagccacatc gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt
    15241 tgccaaaaat aaccagaaac gatttccagt catttttacc aaaacgagta aagcaccaga
    15301 cttcatctac actttgtatt atgctaaaga gagttcaacg atttcgtaag aagcaatgaa
    15361 aaaattgcaa aaagttgtgc agataacata aagtttgttt aagaaagggt aaccttagaa
    15421 ttccgtcgtg aatatgattt gagcacttag atggatagaa aaaagttctc ggaaaacagt
    15481 tcgtttttcg agaacttctt tttatgataa gaatgaaaca aattaactta tattatagtc
    15541 tcttttggta gggaaccatc ttatttttaa tgaaaatcaa gcattttagc tattgaagaa
    15601 tgttgattta tagggattcc ccctccatat tatgttggct ctttattccc actccactgt
    15661 tgctggtggt ttagaagtga tgtcatacac gatacggttg acgtgggcta cttcgtttac
    15721 gatacgtaca gagatttttt gcaagacttc ccatgggata cgggcaaagt cagcggtcat
    15781 tccgtcgatt gaagttacag cacggatacc gactgtgtag tcgtaagtac gtccgtcacc
    15841 cataacaccg acagagcgga tccctggaag aacagtgaaa tattgccaga tatcacggtc
    15901 aagtccagca gctgcgattt cttcacgcaa gatagcatca gattcgcgta cgatttctag
    15961 cttgtcttca gtgatttcgc caagaacacg aataccaaga ccaggaccag ggaatggctg
    16021 acgccaaacg attgaatcag gcatgcctaa ctgagtccct aattcacgga cttcgtcttt
    16081 gaataatgtg tttaatggtt cgatcaattc aaattgcata tcttctggca gtccacctac
    16141 attatggtga gatttgatcg tctgtgctgt ttctgtacca gattcgataa cgtcagtata
    16201 caacgttcct tgtgctagga aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc
    16261 atcaaaaaca tatacgaatt cattcccaat gattttacgt ttttgttctg ggtcagatac
    16321 acctttcaac ttgtctaaga agcgatctct tgcattgact ttgatgatgt tcaagccgaa
    16381 ttttccgcct agactttcca ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc
    16441 aacaaagata gatgtcaatt ggtcaccgat cgctttttgt aaaagaaccc caacgacact
    16501 agaatcaacg ccacctgaaa gaccaagcaa gacttttttg tcaccaactt gttcacggat
    16561 tttttctact tgcatatcga taaagttatc cattgtccag ttaccttcac agtgacaaac
    16621 atcaaaggca aagtggcgca gcaattcgat tccgtattcg gaatgacgta cttctgcatg
    16681 gaattggata ccataaaaat tacgtgttgt gtcttggata gaagcaatcg gacaatcttt
    16741 gcttgttccg actgtttcaa agccttcagg aactttagtt accaagtcac catggctcat
    16801 ccatactgtt tgttgttttg gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc
    16861 taacaaggct ttcccatatt cgcggttttt agcaggctct acggaaccgc caagtttgta
    16921 cgtgatcaat tgcataccat agcaaatccc taatacagga atacctaaat tgaagatctc
    16981 aggatcgatc ccaaatgcat tctcatcata tacactgttt ggtccgccag aaagaatgat
    17041 tccctttgga ttcatcgctt ttacttcttc tgcagtaatg cggtggctca ataattcgga
    17101 aaagactcca agatcacgaa tacgtcttgt gatcaattgg ttatactgac taccgtagtc
    17161 taatacgata atcttttcaa cgttcgtcat atcggcaacg tttgtcaaat ttattacccc
    17221 tatcccttta aattttatct caaaatagtc aaactatttt aaaatgctaa tattttcgca
    17281 tgaaaatcga atcgatgata tgattttcag ttttatggag aattatatcg gctcttcctc
    17341 gtgtaggcaa gatatattcc tgtaaatttt tcaaattcac atttttccat acatcttttg
    17401 ccattttaaa ggcttcttca cgatctccga tagcaaaacg ataataataa ttctctggat
    17461 tttggaaagc cgtatccagt aacgcaccaa aacgatccaa ataccatttt tcgatcagca
    17521 cgggatcggc atcgacatag acagaaaaat caaaaaagtc gctgacgtag atttgctgat
    17581 tcgcaggtaa ttgaagcgta ttgatcccct caacgatcaa gatatcaggc tgctggatca
    17641 attcgtactt gtctggaatg atatcataca catcatgtga gtaaacaggt acttttatct
    17701 cttcctgacc agattttaca cggttcaaga aatcgatcag catctccata tcataacttt
    17761 caggaaatcc tttacggtcc atgattccac gctcttttaa tatacgattt ggataaagaa
    17821 atccgtcagt cgtgatcagc tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct
    17881 gcagcagtct ggctgttgta ctttttccaa cggctacact tccggcgatc ccgataataa
    17941 aaggcggtac ttctacgtat ttatgaagaa ataaaccttt actcaagaac aaagattcaa
    18001 attctttcat ataaagataa atcagatgag caagaggtac atagatatct cgaacatcct
    18061 gcatggaaat gcggtcattc aaacttttga tattatctag ttcatcttgt gtcaaaggtg
    18121 cttgcccatt acgataaaat tctcgccatt cttctcttgc cacacggtag tagttcattg
    18181 gatcattcat attagcctcc tgcgttctcc catcaatgga actattttaa cacaaaataa
    18241 acgcaaaccc caccacttaa gagagattgc cgatcttttt gtataatctt tataatagaa
    18301 agaaacggag tcttattctt gacttgcgca cggatacata aaaggataga ctgttgtcat
    18361 gaaagaaatg agaaggatgg aagacatgac aaatcattac tattctgaac gcccagatac
    18421 ggcgcacgat tttgaacaat gggcttttga gttaaaaggc agaacctttc aatttgtaac
    18481 agattctggt gtattttcac gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc
    18541 tttcgattgg gaagagttac ctgatgaagg aaagatctta gatgtgggat gcgggtatgg
    18601 accgattggg ttagctttgg catttgcgac acagcggttt gttgaaatgg tcgacattaa
    18661 tgaacgagcg gtaagtctag cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga
    18721 tatccatcaa tcgaatatct atgaagcagt tcatgaagaa acctatgcag ctatcgtaag
    18781 caatccgccg atccgtgctg gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc
    18841 tcggttgaag aagggcggta cactgacaat tgtgatccag aagaaacaag gagcgccgag
    18901 tgcgcaaaag aaaatggaag aaacatttgg taatgcagaa atcgtcacaa aagataaagg
    18961 atattatatc atcaaaagtg tgaaagaata actagctgaa aaatagtgga gcgacgaaag
    19021 agtggaactg ggcgtgcatg tacgggtacg tatcagttct ttttttgttg agaagttttt
    19081 aaaaacaaag aactattttt tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt
    19141 acgataaaat aaataagata cagaacttgt atagaaggct gccaagtcgg tagataaagg
    19201 aggtttttat caaaaaacat actttccttt tttctctatt cttttcttgg gggttttggt
    19261 acactgatag ggaatttaga tagaagggag ccaataggat gaagctgacc atcagagata
    19321 tcgcagaaat ggcaggcgta tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa
    19381 gcagatttag tgataagaca agaaaaaaag tgttgtcgat cgtgaatgaa catcattata
    19441 agcccgatta tttcgcttca aatgtgatca atcgacattc aaagaccatc ggaatgatcg
    19501 tccctgatgt cactgatttc ttcttctcca aactgatcga aggtgtagag agctatttga
    19561 atcctttggg ctatgtgatc atgttatgca attctcgtca tagccaagaa aatgagatca
    19621 agtatttaca agagctttcc caccgctcag tggacggaat cttgctggcc acaccaaata
    19681 ttttacctga gaaatatgca ttagacagcg gtttttatca aaaaatgcca atcattttaa
    19741 tcgatcgcgg attgaatcgt cgggataacg gacggcttat cgtaaaggaa tatgagggcg
    19801 cctatcaagc tgtttcgctg tttattgaaa atggccatac gaagatcggc atgctgaagg
    19861 aaaccactgg atactatcaa ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag
    19921 atcatggcct gccttttaat gggaaatttg ttgaacatgg agagttgact gtccaaggtg
    19981 gatatactgc atcaaaaaaa ttactcaaac ataaagatat cacggctatt ttttgcggga
    20041 atgatgccat ggcgatcgga tgctatcagg cgatcgatga attgggcaaa aaaatcccag
    20101 aagatatctc agtcatagga ttcgatggat taaagctgtc agaatacatg attccgcaat
    20161 tgacgacagt gcagcagcca agttttgata ttggatttta tgctgcacgt ttcctgatcg
    20221 atacgatcga atttcctcaa agaaaagttc cgaacaaagt gttcgaaaca aaattaatta
    20281 ttcgtgagag cgtaaaagta ttgacaaagg gctaactcgg cggtatattg cttatgtaag
    20341 catttacaaa ggctagttag tgcttttttt atttttagta aaccagttta ctaaagtggt
    20401 ttacctttcc taaaaataat gtataatacc gaatgaagac aaggaggatc aggcaatgcg
    20461 aatggtggat ctgattgaaa aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga
    20521 gtacatcgtc acgaactata cgaatggaaa gattccagat tatcaagtca gtgcattatt
    20581 gatggcaatt ttttatcaag acatgacaaa tgaagaaatc acgaacttaa cattggcgat
    20641 tgctaattct ggtgatgtca tcgatttgag ttctcttgaa ggaataaagg tagataagca
    20701 ttcgacaggt ggcgttggtg acacgaccac attgatttta gcccccctag tagctagtgt
    20761 cggagtgaca gtggctaaaa tgtctggacg cggtctaggt tacacaggtg gtacactaga
    20821 caagctagaa gcaattcccg gatttcagat cgagctttcg gatgaagaat ttgtacgcat
    20881 tgttaatgaa agtaaagtag cggtaattgg tcaatcagga aacttggcac cggcggacaa
    20941 aaaattgtat gccttgcgtg acgtcacagc gaccgttgat tctcttccgc tgattgccag
    21001 ttcgattatg agtaaaaaaa tcgcggcagg agcagatgca atcgttttgg atgtgaccac
    21061 tggagacggt gctttcatga agaacatcga agatgcaaga cgtctggcaa agaccatgac
    21121 gagcattggg aaactggcta accgagaaac agtggcggtt atctcagata tgtcagaacc
    21181 tcttggcgaa gctatcggaa atagtctaga agttgtcgag gcaatcgaaa cattacaagg
    21241 aaatggtcca gaagatctag tagaaatgtg ctacgcctta ggaagccaaa tggttgttct
    21301 tgctggcaaa gctaagacaa ccgatgaagc aagaacgttg cttcaagaag ctctggaatc
    21361 aggtaaagcg ttagcaaaat tcaaagaaat gatccaaaat caagggggag acccagcgat
    21421 cgtggaacat ccagaacgaa ttttgactgc acgctacaca atggagcttc ctgctaaaca
    21481 aagcggagtt gtgtcaaaaa tcgtggcaaa tgaattagga attgctgcaa tgatgctagg
    21541 tgctgggcgt aaaaccaaag aagaagatat tgatcacgct gttggattga aactgcataa
    21601 aaaaattggt gacactgtga caaaaggtga gtcactattg acgatctata gtaacgacga
    21661 agaaatcact tctgtcattg aattattata taaaaatatt gaaattggtg aatcggcaat
    21721 gaagccaacg ttgattcatg acatcatcac agaataaagg aaaaaagcgg actttgccgt
    21781 tttaaagaaa aggggaaaag aaatggaact aaaccgaatg atcgaccata ctattttgaa
    21841 agcagacgca tctaaagaag ctgtgatgca aattatcgaa gaagcaaaaa aataccattt
    21901 ttattctgtc tgcatcaatc cagcatgggt tgcgctagcg aaagaacaat tacaaggtga
    21961 gccagtagcc gtatgtacag taatcgggtt tcctctagga gcaaacacac ccgagacaaa
    22021 agcatttgaa acaacagatg ccatcaataa cggtgcagat gaagtcgata tggtcatcaa
    22081 tatcggtgct ttgaaatcaa aggatgatca acaagtccaa aaagatatcg aagcagttgt
    22141 tgaagcagca aaagatcgtg cgcttgtcaa agttattatt gaaacatcgt tattagatag
    22201 agaagaaatc atccgagctt gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac
    22261 atcaactggt ttctcaacag gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac
    22321 tgtaggacca gaaatgggtg tcaaagcttc cggcggtatc cataacgaag aagaagcaat
    22381 ggcaatgatc gaagctggtg ctacaagaat cggaacaagc gcaggagtag caatcgtatc
    22441 tggttcaact ggtgaaggct attaattttt tcaaaggaga agctcatggc agaactcaaa
    22501 caagaatgga tcaacacagc tattgaagcg ttagataaag cctatgttcc ttattcccat
    22561 tttccggtag gcgcttgtct cgtaacggaa tcaggcaaga tttatcaagg catcaacatt
    22621 gagaatgcat catttggatt aacgaattgt gccgagcgca cagctttttt caaagcagta
    22681 tctgaaggag aaaaaacatt tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc
    22741 tctccttgtg gtgcatgtcg acaagtaatg gcagaattct gttcccctga tatgccggtt
    22801 acgctagtag gagacaatgg tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat
    22861 gcatttactg aaaaagatct ataactcatg ttcattcagt cagctgactg ttgaaaaata
    22921 aaacaattat tttaggaggc tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg
    22981 ctgtcgcact agcagcaggg ttatttttag gtgcatgcgg caacaacggc tctactgatt
    23041 catccggtgg caaaagctca tcagacacaa caactgcagc attgatcaca gacacaggtg
    23101 gtgtagacga ccgttcattc aaccagtcag cttgggaagg actagaaaaa tgggggaaag
    23161 atcaaggact ttcacgagga aatgatggct tccaatattt ccaatcttca aatgaatcag
    23221 attacatccc aaatattgac caagcattga atgctggatt caaaacgatt tttggtattg
    23281 gttataaact aaaaccagcg atccaagaac aagcaacaaa caatacaggt acgaactttg
    23341 ttattatcga tgatgtcatc gatggattag ataacgttgt atctgcaact ttcaaagaca
    23401 acgaagcttc ttatcttgca ggtgttgcag cagcttatac gactgaaaca aacgtagtcg
    23461 gctttatcgg cggtgtaaaa ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg
    23521 gtgttgatgc aggtgcgaaa gaattaggaa aagaaatcaa agtattgaat caatatgctg
    23581 gtgacttctc tgctccagat aaaggacgtt caattgctca aggtatgtat gcacaaaacg
    23641 cagatatcat cttccatgct tcaggcggta caggtaacgg tgtattccaa gaagcaaaat
    23701 cattgaacga atcaggcgac aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag
    23761 acgaaggcga atacacatta aacggcgaaa agaaaaactt cacattgaca tctacactga
    23821 aagcagttgg aacagtagta gaagatttgg cacaaaaatc agcggacggt aaattccctg
    23881 gcggcgaaca cactgtttat ggattgaaag aagacggtgt tggtctaaca gaaggacaac
    23941 tttctgacga agcgaaaaaa gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg
    24001 taaaagttcc agaaactcct gaagaaaact aaaaccattt ctatagcctt gttagacaca
    24061 tgtttgacaa ggctatggtt tgattagaac aagaattgtt catttatcca agctgccttg
    24121 tgacagtttg gataagggaa caactccctg aagtaatgtt tgtttcaaaa gaagaacgct
    24181 cactaagcgt gaatcttagt caatcgttgc cctagataga aatcgttagg atgtgaaaaa
    24241 aaggtggttc aagaaaatat tgtcatagaa atgcagaata ttaccaaaga atttggaaca
    24301 ttcaaggcaa atgacaacat caatttacaa gtaaaggcag gagaaattca tgcgttgctt
    24361 ggagaaaacg gtgctggcaa atctacattg atgaacgtgc tttccggatt attagagccg
    24421 acatcaggga aaattttgat gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc
    24481 aaccaattag ggattgggat ggtccatcag cactttatgc ttgttgatgc ctttactgta
    24541 acagaaaaca tcgtgttggg aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa
    24601 gcacgaaaag agatccaaaa agtttctgaa cagtatggat tgtcagtcaa cccagatgct
    24661 tacgttcgtg atatttcagt tggaatggaa caacgggtag aaattttaaa aacactttac
    24721 cgaggagcag atgtactgat ttttgatgag ccgacagctg tattgacccc tcaagaaatt
    24781 gatgaattaa tcgtgatcat gaaggaatta gtcaaagaag gcaagtcgat cattttgatt
    24841 acgcataagt tagatgaaat caaagcagta gctgaccgct gtacagttat ccgccgtgga
    24901 aaaggaatcg gtacagtcaa cgttaaagac gtaacctcac aacaattagc cgatatgatg
    24961 gttggaagaa cggtttcatt caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt
    25021 ttgtctattg aaaatctagt ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg
    25081 aacttagagg ttcgtgctgg cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag
    25141 tcggagttga tccaagcttt gactggtttg cgaaaggcag aaagcggaca tatcaagcta
    25201 aaaggggaag acatcactaa taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac
    25261 gtgccagaag accgtcataa atacgggtta gtcctagata tgacattatc tgaaaatatc
    25321 gcactacaaa cctatcatca aaaaccttac agtaaaaacg gcatgctgaa ttattcagta
    25381 atgaatgaac atgccagaga attgatcgaa gaatatgatg ttcgaacaac gaatgaactt
    25441 gttcccgcaa aagctttatc aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc
    25501 gaccgagatc ctgatctgtt gattgttgca aatccaactc gtggactgga tgtaggtgcg
    25561 atcgaattta ttcataaacg tctgatcgaa caaagggaca aatacaaagc agtgttactg
    25621 atcagtttcg aattagaaga aattttaaat gtttcggatc gtattgctgt tatccatgaa
    25681 ggagaaattg tcggtatcgt tgatccgaaa gaaacatctg aaaatgaatt aggtctattg
    25741 atggcgggct ataccttaga agaagcacga aaagagttag aaaagggggc tggcgcacat
    25801 gaatgatcgc agcgaaaaat tccgtaattt attagtgcct gtactttcag ttgttttagg
    25861 attgatctta ggcgcgatat tgatggctgc ttttggtttt cagcccatcc aaggttattc
    25921 ttctatgcta gatgcagccc ttggttcgca aagagggata ggtgaaacgt taaggcaagc
    25981 aacgccgttg atttttacag cattaggttt ttctgtagct aactcagctg ggtttttcaa
    26041 tattgggctt tctggacaag cactttgcgg gtggatcgtc agtgtctggg tggcacttgc
    26101 gtttccagat ctgccaaaag ttgttttgct tccgttatgc gtgatcttag gtgccttagc
    26161 tggtgcgatg gcagcggctg ttccaggttt acttcgagca ttttttggaa caagtgaagt
    26221 gatcgtaaca atcatgatga actatatctt attatacgta agcacatttg ctttacatga
    26281 agtgatgcct gaaagctatc gctcaagttt ggactcttcc aatatgatca gtcaaaatgc
    26341 ctcactgcgg ttaccatggt taacacaagc ttttggcggc tctcgcgtaa actcaggatt
    26401 atttttagca atcatcgcgc ttattttcgt ttggctgctg atgaagaaaa cgactttagg
    26461 atttgaaatc cgttctgttg gattgaatcc ttttgcttct gagtatgcag gaatgagtag
    26521 taaacgtacg atcgttttgt cgatggtcat ttctggcgct ttggcagggc ttggcggcgt
    26581 ggttgaagga ttaggaacgt atcaaaactt ctttgttcaa acgacttcgc tatctattgg
    26641 atttgacgga atggctgttt ccttgcttgg ttctggatca gcaatcggta ttcttttatc
    26701 agcgatcttg ttcagtattt tgaagatcgg tggacttggc atgcagacag gagcaggtgt
    26761 tccgtttgag atcgtcaatg tatcgattgc tttgattatc ttctttgtcg ggatcaactt
    26821 cctgattcgt ttcgtcatgg ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc
    26881 gacaatagaa tcgaaaccaa cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact
    26941 atcgaattaa ttgccttgat ccttacttct acactggttt attcaacacc gctaattcta
    27001 acgtcactag gcggaacttt ttctgaacga agcgggatcg taaacgtagg gctagaggga
    27061 atcatggtca tgggtgcttt tagttccatc gttttcaact tgacgatgag tccatcactt
    27121 ggttctatga caccatggat cgctatttta gtgggtggcg tcgtaggtat cctattttct
    27181 ctgattcacg cagtagcaac agtcagtttg cgtgctgacc atattatcag cgggacagta
    27241 attaatctga tggcacctgc tttaggtgtt ttcttgatca aagcatggta tggtaaaggt
    27301 caaactgata acatcacgca gaatctgggg tatttttctt tcccaggatt agcagatatt
    27361 ccggtcattg gaccaatttt ctttaaaaat acattcttac ctgcttattg tgctattttg
    27421 attgctgtcc tagcttgggt gatcatcttc aagacgaaat tcggcttgcg cctccgttca
    27481 gttggtgaaa atcctcaagc tgccgacaca ttaggtatca atgtatatgc tatgcgttat
    27541 tcaggcgtac tgatttctgg cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg
    27601 atttccggta acttttcagc tggtacgatc gttggacaag ggttcatctc catggctgct
    27661 atgatctttg gtaagtggaa tcctttaggg gcaatgggtg cctcattgtt ctttggtttt
    27721 gctcaaagct tgagtatcat tggcgcacaa ttaccagtta tttcttcgat tccgccagta
    27781 ttgctgcaaa ttgctccata tttgctgaca atcattgtgc tggttgtttt cttagggaaa
    27841 gcttctggtc caaaagcaaa cgggaagaac tatatcaaat caaaataatc ttttagcgaa
    27901 taaaaagggt gggttacggg aagtgttttt cgtctcgccc tttttgtttt aaaggcaata
    27961 aaaataaaaa gaaagaagga ataatcatgt ttaaacgcgt acatctgatc gtcatggatt
    28021 cagtaggaat tggagaagca ccagatgccg ataagttcgg tgatgttggt tctgatacat
    28081 taggacacat tgcgaaagaa gcaggattga acgttccaca tttagagcag cttggtttag
    28141 gaacgatccg tcctcttgaa ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga
    28201 aattagaaga agtttctgta gggaaagata cgatgacagg ccactgggaa atcatgggat
    28261 tgaatatcaa aactccattt cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac
    28321 aaatcgaaga attttctggc cgtaaagtcg tatgtaacaa accttatagc ggaacagctg
    28381 ttattgacga ttacggtgaa caccaaatga aaacaggcga tttaattgta tatacttctg
    28441 ccgatcctgt ccttcagatc gcggcacatg aagaaatcat tcctttagaa gaattatatc
    28501 ggatttgcca attcgttcgt gatataacaa aagatgatcc gtatatgatt ggccgaatca
    28561 ttgcacgtcc gtacgtgggt gaaccaggag acttcacacg aacaagcaat cgccatgatt
    28621 atgcgttgaa tccattcgga cacacagtcc ttgattcttt gaaagaagca ggaaaggacg
    28681 tgattgctgt tggaaagatc aacgatattt tcaacggaca aggaatcact gaatctgtcc
    28741 gcacgaaaag caatatggat ggcgttgacc agctgctgaa agttatgaaa aacgattttt
    28801 caggaatcag ctttaccaat ttagtagatt ttgatgcatt atacggtcat cgtcgggatg
    28861 tagtcggcta tgcgcatgcg attgaagaat ttgacttacg aattccagaa ttgcttgaag
    28921 cgatggaaga agacgacttg ctaatgatta cagccgatca cggaaacgat ccgacgttta
    28981 ctggtaccga tcatacgcga gaatatgtac cgttattggt ttacagtaaa aaaatgaaag
    29041 aacaaggggc attgcctcaa ggatattatt cagatatttc agcaacgatt gctgagaact
    29101 ttagtgtttc ttcgactgaa aatggcgaaa gcttcttgaa attattaaaa taatcttttt
    29161 aaataggaga aagcaaatga ccaagttaag cgagatgtta caagaaacga cccagtttat
    29221 ccttgataaa ggagtaaaag aaattgattt tggtttgatt ctaggttcag gattaggtga
    29281 attagcagat gaaatcgaag atgctgtaat gatttcttat gatgagatcc cgttctttcc
    29341 tgtttcgaca gtagttgggc atgcaggaaa gctagtttat gggtctctgg caggcaagaa
    29401 agtgttagcc atgcaaggga gattccacta ctatgaaggt cattccatgc aaacagtgac
    29461 ctatccagta cgtgtgatgg ccggactagg ggcccattca gtcattgtga ctaatgcatg
    29521 tggtggtgtc aacgaatcat ttgtaccagg agatttgatg ctgattaccg atcatatcaa
    29581 ttttactggt gaaaatcctt tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc
    29641 agatatgagt caagcttata cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa
    29701 actaggtttg gatcttaaag aaggcgtata tatgggctat tctggaccaa cttatgaaac
    29761 gcctgcggag atccgaatgt ctcggacaat aggagccgat gcagtaggga tgtcgactgt
    29821 tccagaagtc atcgtagcag ctcacagcgg attaaaagtg ttaggaatct cttgtattac
    29881 caacttagca gcaggtatgc aagcgaatct gagccatgaa gaagttgtag aaacaactca
    29941 gagagtcaaa caatctttta aagcattggt taaagaaaca ttgacattat tgtaatagga
    30001 ggaacaataa aatgagtgtg catattgaag cgaaagaagg cgagatcgcc gataagattt
    30061 tacttccggg agatcctttg cgagctaaat atattgcgga gacttttttg gaagatccag
    30121 tttgttacaa tcaggtacgc ggtatgttag gctttaccgg caaatataaa ggagaacgta
    30181 tttctgttca aggaacagga atgggaatgc catcagccac catctatgcc catgaattga
    30241 tccagtcgta tggtgtaaag aaattgattc gagtaggtac atgcggtgca ttgtcaaaag
    30301 acgtccatgt tcgtgattta gtactagcac aaggtgcagc gaccagttct tcgatgatcg
    30361 aaaaaaattt ccaagccttt cattttcctc caatcagtga tttcaatcta ttactgaaag
    30421 catatgagat agctaaagaa aagggctata cggttcatgt aggaaatgtt ctttcggaag
    30481 attctttcta caaagatgat ttgacagaga cgttccaatt agctgaactt ggcgttttag
    30541 gagtggaaat ggaagcagcg gctctttatt acttaggcgc gaaataccat gtgcagacat
    30601 taagtttgat gaccgttagc gaccatttga tcaccggtga agaaacaaca gctgcagaac
    30661 gtcaatctac gttcaatgaa atgatcgaag taggattgga aacagcgata gcagaataag
    30721 aaggaaatga aaaagctgcc ctgcattaag taataagaac atccaagcaa aaatagctcc
    30781 tcatgttttt gacttggctg gacttattat tgcaaatgta agcttttgaa caccgtttac
    30841 caagaggttg tgaatctgcc gtttaactct gagtcacaag gaacaatttc aaaaaacagc
    30901 ttttcatgtt tgatgaatat tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt
    30961 atacgaaaaa agggactgtg acaaatcgtg tcacagtccc ttttttatag tgttttttat
    31021 aggtagtatt ttttagcaac tgttaagtca tcatttaatt cataaacaag tggttgacct
    31081 gttggaattt caagatccat gatgtcttcg tcagagatac cttcgatatg tttagctagg
    31141 gcgcgtaatg agttaccatg tgcagctact aaaacagttt tgttgtctag taatgctgga
    31201 gcaatttcat cttgccagaa tggcaaagcg cgttctaatg tcactttcaa gttttctcca
    31261 ccaggaatat cgcgtttgtc taacattgcg taacgacggt catttgctgc agaaccttcg
    31321 tcagtttctt ccattaatgg aggaagagta tcgtaagaac gacgccagat gtgcacttgt
    31381 tcgtcgccgt atttttcagc tgtttctttt ttgttcaaac cttgtaattt accatagtga
    31441 cgttcgttta agcgccatga tttgatttgt ggtacccaaa gttgatctga atattctaag
    31501 atcaagttac atgttttgat tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg
    31561 attccagctt ctttgatttt gcgtccgcct tcttttgctt cttcgattcc ttctggtgct
    31621 agatttacat cagcccaacc agtaaattgg ttcaatgcat tccattcact aagaccgtga
    31681 cgagaaaaaa ctaattttgg catgtttagc tctcctttta agataggttt atatttctga
    31741 aattttcagt tctgttttat tgtacaattt tttggaagag atttccatcg aaaaattaga
    31801 taagattcta tgagaaaaca agtagagaat ataaaaaaac gaacagtgcc cacgcaactg
    31861 ttcgttttta ggtattagat gcataattgc tcgatttctt tttcaggtaa attgatatat
    31921 ttataacatg ttccaactga aacaccgcat tttgaagaaa taaattggat cgtttctttt
    31981 ttttcataat agagacgtcg gatttttttg atcgtttttg catcgatttt tggtcttccg
    32041 ccgatttttc cttttttacg agcttcatct aatccaacta atgtacgttc tttgatcaat
    32101 gcgcactcca tgtttgctag gttttccatt aattggaaat agatttttcc catttcgcgc
    32161 gtatcgattc cttcatcaag actaatcaag tgcagcccgc gatctttgaa taattgcgtg
    32221 agttctgtta attgccgcgt agatttgcct aatcgatgaa gttggcaaat gactagtgca
    32281 tcgccttgtt gcatgtcagc gatcactgac tccaaatgaa cagcagtaga atcattttgt
    32341 tccacttcat atgtctcatg gaaaatttct tgacaaccaa agtcagataa ttgatcaacc
    32401 tgagttgtta aatcaacgtc atttatggtt gtgcgtgcat aaccaataat tttcatggtg
    32461 attcctttct agcagtgatt ttctaactgg atataacagt aaaaatgtag cataaagtac
    32521 gtcaaaaaga tagttttttc gcaaattttc cgctgtcttt ttttattgga tgatcaagca
    32581 gtttttcttt atttacgacg gattgcgtca agaatctcaa cgcctttttc tgagccgaca
    32641 actactttag aaaccatgtt taggaaaagc ccatgttcta ctaccccaac aagttcatct
    32701 aaataggtag ctaagctttc tggctgttcg atgacttcta gatgcagatc aatgatgtaa
    32761 tgaccaccat ctgtggttaa gacttctccg ttttcgtctg tacgcaagac gggttggaat
    32821 cttttttctt caaaaatatg aagtagctgt tgggagccat aagggatcac ttcaactggg
    32881 agtgggaact tcccaagttt atcgactaat ttagaagaat caacgatcca aatcgtttct
    32941 tttgagtacg tagcgactat tttctcgaaa agcaaagcag cgccgccgcc tttgatccct
    33001 tgaaaatcag cactgatttc atcagcaccg tctattgtca aatcgactac ggggacttca
    33061 tcaatactct ttaacggtat tcctaatttt tccgcttgtt cttttgtcgc atttgaagta
    33121 gtaactcctg tgatacgtaa accttcttca cgcatacggc gaccaatctc ttctaccata
    33181 taataggcgg ttgaaccggt cccgagacct acgatcatcc cgtcctttac gaatttcgcg
    33241 gcttcaatgc cagctaattg ttttaaattc atctttgcgt ccaccttttt ttagtttgag
    33301 catcgtgctt tcctcactat tttagcgtga tttacggtaa aaagatacca ttctttttta
    33361 ttccaataaa gctttttcaa aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg
    33421 atattctaac aaaggtgctt tatctacagg tttctgtgaa gaaacgtgct gactgtttat
    33481 caaagcatat ttgataagga tgcagacatt gcatttattt tttatcacaa aaaaagaacc
    33541 acctggatgt gtggaactag acgcaaatcg tgggaaggag gaaacaagga atgcctacaa
    33601 ttaaccaatt agtacgtaaa cctcgtaaat caaaggtgga aaaatctaat tcacctgctt
    33661 tgaacaaagg atataacagc tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac
    33721 gtggggtagc aactcgtgtg ggtacaatga cacctaaaaa accgaactca gctttacgta
    33781 aatatgcacg tgttcgtttg tcaaacttaa ttgaagttac tgcttatatc ccagggattg
    33841 gtcataactt acaagaacac agtgttgtat tattacgcgg tggtcgtgta aaagacttac
    33901 caggggtacg ttaccatatc gttcgcggtg ctttagatac tgctggtgtt aacgaccgta
    33961 aacaaagccg ttctaaatac ggaactaaaa aacctaaagc ttaattttaa tta
//
LOCUS       DAXWLF010000010        33670 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXWLF010000010 DAXWLF010000000
VERSION     DAXWLF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.010"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..629)
                     /locus_tag="ACGE4U_000353"
     CDS             complement(<1..629)
                     /locus_tag="ACGE4U_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8392544.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRL"
     gene            777..2066
                     /locus_tag="ACGE4U_000354"
     CDS             777..2066
                     /locus_tag="ACGE4U_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8392545.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(2192..3271)
                     /locus_tag="ACGE4U_000355"
     CDS             complement(2192..3271)
                     /locus_tag="ACGE4U_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8392546.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3380..3820)
                     /locus_tag="ACGE4U_000356"
     CDS             complement(3380..3820)
                     /locus_tag="ACGE4U_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8392547.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3877..4287)
                     /locus_tag="ACGE4U_000357"
     CDS             complement(3877..4287)
                     /locus_tag="ACGE4U_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFF8392548.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4468..4902)
                     /locus_tag="ACGE4U_000358"
     CDS             complement(4468..4902)
                     /locus_tag="ACGE4U_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8392549.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(5002..5850)
                     /locus_tag="ACGE4U_000359"
     CDS             complement(5002..5850)
                     /locus_tag="ACGE4U_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8392550.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            6239..8005
                     /locus_tag="ACGE4U_000360"
     CDS             6239..8005
                     /locus_tag="ACGE4U_000360"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFF8392551.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            8107..9366
                     /locus_tag="ACGE4U_000361"
     CDS             8107..9366
                     /locus_tag="ACGE4U_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8392552.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            9513..10814
                     /locus_tag="ACGE4U_000362"
     CDS             9513..10814
                     /locus_tag="ACGE4U_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8392553.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10817..11668
                     /locus_tag="ACGE4U_000363"
     CDS             10817..11668
                     /locus_tag="ACGE4U_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFF8392554.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11681..12496
                     /locus_tag="ACGE4U_000364"
     CDS             11681..12496
                     /locus_tag="ACGE4U_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFF8392555.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            12647..13813
                     /locus_tag="ACGE4U_000365"
     CDS             12647..13813
                     /locus_tag="ACGE4U_000365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8392556.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            14043..14690
                     /locus_tag="ACGE4U_000366"
     CDS             14043..14690
                     /locus_tag="ACGE4U_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFF8392557.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14774..15718
                     /locus_tag="ACGE4U_000367"
     CDS             14774..15718
                     /locus_tag="ACGE4U_000367"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFF8392558.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15853..17304
                     /locus_tag="ACGE4U_000368"
     CDS             15853..17304
                     /locus_tag="ACGE4U_000368"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFF8392559.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            17325..18161
                     /locus_tag="ACGE4U_000369"
     CDS             17325..18161
                     /locus_tag="ACGE4U_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8392560.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            18161..18820
                     /locus_tag="ACGE4U_000370"
     CDS             18161..18820
                     /locus_tag="ACGE4U_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8392561.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            18946..19635
                     /locus_tag="ACGE4U_000371"
     CDS             18946..19635
                     /locus_tag="ACGE4U_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392562.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            19641..20597
                     /locus_tag="ACGE4U_000372"
     CDS             19641..20597
                     /locus_tag="ACGE4U_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFF8392563.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            20641..21153
                     /locus_tag="ACGE4U_000373"
     CDS             20641..21153
                     /locus_tag="ACGE4U_000373"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8392564.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(21409..21957)
                     /locus_tag="ACGE4U_000374"
     CDS             complement(21409..21957)
                     /locus_tag="ACGE4U_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFF8392565.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(22046..23227)
                     /locus_tag="ACGE4U_000375"
     CDS             complement(22046..23227)
                     /locus_tag="ACGE4U_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFF8392566.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(23463..24971)
                     /locus_tag="ACGE4U_000376"
     CDS             complement(23463..24971)
                     /locus_tag="ACGE4U_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFF8392567.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            25130..25321
                     /locus_tag="ACGE4U_000377"
     CDS             25130..25321
                     /locus_tag="ACGE4U_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392568.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            25524..26441
                     /locus_tag="ACGE4U_000378"
     CDS             25524..26441
                     /locus_tag="ACGE4U_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFF8392569.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            26434..27507
                     /locus_tag="ACGE4U_000379"
     CDS             26434..27507
                     /locus_tag="ACGE4U_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392570.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            27655..28173
                     /locus_tag="ACGE4U_000380"
     CDS             27655..28173
                     /locus_tag="ACGE4U_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392571.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            28154..28546
                     /locus_tag="ACGE4U_000381"
     CDS             28154..28546
                     /locus_tag="ACGE4U_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFF8392572.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            28622..29557
                     /locus_tag="ACGE4U_000382"
     CDS             28622..29557
                     /locus_tag="ACGE4U_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8392573.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            29690..30469
                     /locus_tag="ACGE4U_000383"
     CDS             29690..30469
                     /locus_tag="ACGE4U_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFF8392574.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            30462..30779
                     /locus_tag="ACGE4U_000384"
     CDS             30462..30779
                     /locus_tag="ACGE4U_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFF8392575.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            31023..32633
                     /locus_tag="ACGE4U_000385"
     CDS             31023..32633
                     /locus_tag="ACGE4U_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8392576.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32790..33638
                     /locus_tag="ACGE4U_000386"
     CDS             32790..33638
                     /locus_tag="ACGE4U_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8392577.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        11156 a         6010 c         6544 g         9960 t
ORIGIN      
        1 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
       61 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
      121 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
      181 atagacaaac cgtacaatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
      241 gaggcactat agtttgtttg ctgacttaaa ctgataatcg aatttgtctg acagctgaac
      301 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
      361 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
      421 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
      481 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
      541 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagaga
      601 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
      661 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
      721 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
      781 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
      841 agacagtcca cacagaggag atagaaacag ccaaatggac agaaaccaag tgggccaacg
      901 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
      961 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcaggagac ttagcaccta
     1021 tactacaatc agctgttaca tttgagaatg ttgatcccgc tttgtcgatt ggctatgacg
     1081 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     1141 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     1201 atcatcattt aacgccgtat agtcagcggc agacaattga gcaagattca gctatcgact
     1261 caggaggaga tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag
     1321 taccgcctac agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac
     1381 ccgatacaga aactcaacca gagccagaca atgaatcaaa acctgaaata acccctggag
     1441 aaaaaccaga tgtagaccca gaagaaaaac cggatgtgac tccagaacca gatacggatt
     1501 ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag
     1561 aaaaaccaga ggtagcccca gaagaaaaac cagatgtaac tccagaacca gatacggatt
     1621 ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag
     1681 aaaaactaga ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg
     1741 caagagacca aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa
     1801 aagagagtaa aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa
     1861 aatcacgaat atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa
     1921 cctttgctag tcctgaaacg aaaaataaac agctaccaaa atccggagag tcacaaaaca
     1981 aagttatact gtggagcgga atcattctat taagtatagc aacaatgctt tctgcaaaaa
     2041 gatttaaaca aaacagatct ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa
     2101 taaaaagaaa tatccagaaa acgaccccaa aataccagat tgaaatctga tatttggggt
     2161 cgttcatact ttatctttct tcatatttat tttaagggat atctgtttct tttggaagat
     2221 ggttcgggat ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga
     2281 caatcaagcc ccaaacgcca aagaaccgtt cgctaacaag taagaccaca aatgtataaa
     2341 aaatcggcag ttctgttttg ctggacatca acttcggatt cagcacataa gactcaatta
     2401 gatggacaat caaaatcgta atcaggatat aaacaacatc tcggatgcct ccttgggaat
     2461 aagctatgaa agatagagga atacaagaaa tgataacacc tgctactggg atcagactta
     2521 aaatgaaaat catgatcgct aaactcagca gctgatgaaa gccaaataca gccaaggcga
     2581 ttgtagtgag caacgtgttt aaaagagcga taacaaattg agcttctaat accaaaccaa
     2641 atgtattgac aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac
     2701 tttttaaaaa caatcttgag aatacaattg tttttttctt ttcaatcata aaaaagaaac
     2761 ttaagatgaa tgacatagca aacgccactg ccatttttcc tatatcctgt aaatagccaa
     2821 gcgcaatcga agcgccattt tgaagctgtt caaaaaaatc gtttcgctca atataagtat
     2881 ggatatattg taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa tcaataacgg
     2941 actggatcat atcaaatgtt tgatgaataa gcatcgggat atactttgtg aatgccagat
     3001 agataaggta aacgatcagt gtataaatca tgatagctaa aacacctgta gggattcgga
     3061 caaatcgctg aatgatttga acaagatgaa tcatcaaata agtaaagata aatgtgagca
     3121 aaatggttgt aatcatactt ctaactaaga aaagaacaaa aacgacaaat aataaaacag
     3181 tgagacggcg tattttttca ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct
     3241 ccaaataaca aaaaataaac acgttattca ttatatgatg gaatatgaaa ataagaaaga
     3301 gaaagagcaa aaagaaaaac cgctaaatga cgttatctta tgagttggtt ttcctcataa
     3361 aatcgttcat ctagcagttt cagccctttt tctggtcttg atgaaaatta tctagcattt
     3421 ttcgggtcag atgttgcagt gtatccaatt cttcctgttc aagtggaaga taagacttga
     3481 tcttttgtgg aataccttct gcttgttttt gcaacgcagc accttgagga gtcagcgtaa
     3541 catttacgat tctttcatca cccgtttcac gctgacgatt aatataattt ttttctgcta
     3601 acttttttag taagggagtc agcgtaccag aatccagaca caaatcattt cctagctctt
     3661 tcactgaagt ctttttcttt tcccataaaa ctaacatcac aagatactgg gtataggtaa
     3721 gattcagcgg cttgaggagg gaagtgtagt gtctaatgat ttccttagag gcggcatata
     3781 aaggaaaaca tacttgatta actaaatata gatcttccat ttttccacct ccgctgtttt
     3841 actatagtgt atcatagcgg atttacacaa aaaagtctat tcactaactg ctttcagagt
     3901 aacttctata tttccagctg tagcttttga atacggacac actttatgcg ctgcctctaa
     3961 taattcttgc gtttcttcca gagaaagacc ttcgatagat ccttcaatct ctacacctaa
     4021 gacaaagctc gtttcacttt ctgaataaag ggaaacttgt gcactgacag tagaagcccc
     4081 agagatattt ttttgctgtt tgatcaattc taatgcactg ttaaaacatg cactgaaacc
     4141 agctgcaaac aattgttcag ggttggttgc attttcgacg cgtttaccag gagctgcaat
     4201 attcaattcg aatgtacgat cgggattatg aacttctcca gagcgtccgc ctgtgttgat
     4261 cattttcgta gaatacattt ttttcataaa attaccacct ttaattatat tttatacaat
     4321 tgaattttac acaatataat tgtggaaagc aaagaaatgg attggcataa gataaataag
     4381 agaaagcaca tgaaacagac gcgctttctc gaatagcaaa catatacaaa aaaatatcag
     4441 agacaaaaac taatctcatc ataaacatta catatatttc tcaaaaagtt gttttctttc
     4501 ttcaaaactt acttcacgat ggacctgatg aagcatccat aaataaccaa aaggatctct
     4561 gaacattgag ttagtcacac caaagtccat catttctgtc atttcttgaa tctctacaca
     4621 gccatttcgt aatgctttat tatgtgtgtc ctcgatatca gagacgatga tattgaacca
     4681 gatcgtattt ggatgttcag cagtaggagc agtcaaacca agagacgtgt tttcatccag
     4741 catatggaat ctcgtgccat acaaggtaaa aatgacttca ttattgcctc tagggtaatc
     4801 tgttgcctca atcagttgta tttcatcaaa gattgtttga tacagttcta gtgctttcaa
     4861 actatcattt aatacaaaat ctatttccgt tcctttatgc ataaagatct cctctccttt
     4921 tcagtcgaac agttactaga ttcttttatt ataccagtta aagaaaacgc attcaaaaaa
     4981 atagcggatt tacttaccaa ctcattcgat tggatgataa gtaaatccgc gtcccattac
     5041 ttctgctacg ctgctgacaa tgacaaaggc acttggatcg atttcttcta tcaatggttt
     5101 cattcgggaa aattcatgat cagtcacaac gatcaataaa acctcttttg actttcgatt
     5161 atacccgcct tttgcctcta gcagggtaag gccacgtcca atctcagcac ttaagcgaag
     5221 gcggatgtct tccaaataat gttcactcat gaccataaca gatttctttc gattgatacc
     5281 tgtttcgata taagtcatga caattgaagt gattgcaata gataaaatag cataaagaaa
     5341 ctcttctatt ccaaaaacaa acagattaag agaaacaacg aatgcgtctg taaaaaataa
     5401 gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg aggggaggga tcgtcgtacc
     5461 gccgctagaa gagttgtttt tatataaaat cgcaactcct aaagcaaaaa tcgcacttcc
     5521 aaaaatgaca gacaacaacc gatctgaagt aatcattgtt tctggaacta ttgcaatgat
     5581 taaaggaaaa aacaagcttc cgaaaagtac tttaaaaaag ggttttttac ctaaaaatag
     5641 aagagctagt accaacataa cgatattcaa cactaaaata actatagctc ggtcaaatcc
     5701 taaaacagct tccagtaata ttcctagacc gctgacacct ccagcagcaa tatgatgagg
     5761 ccctaaaaac atattgatag cgactcctaa tatgaagagg gcaaaaataa tcttaatgta
     5821 tgtccatata aattgtctcg tttctttcat ttatccactt ccttaattca ttaataatac
     5881 tcttatctta ttataactta cttttcacct taagttaact ttaattaata aaaaaattag
     5941 gctagtctaa attattatgt ttcattcttc tagaatatac ttctacgtta aaaactgtta
     6001 aaaaactttt actttagaaa attggagaaa ttgatcgctt aagataaatt caggacacta
     6061 aatagtacag gaagtagatt aaaactttta aaagttttaa gtctatctat ttggcaagtt
     6121 taaaaaataa aaattcggta tcaaaaaact gttaccgata acatttttga aagcgttgtc
     6181 ttttgtttct ctatcactta tagtgatgac gtcatattaa aacacaagga ggaaatcaat
     6241 ggatacagct gcaatttatc atcgaccgga aagtgaatat gcatttctat atacgaatac
     6301 aagatttcgt attcggcttc gtactaggaa aaatgatatc aaaaaagtat atgtgctttg
     6361 tggagatcca tataccataa caactgaaaa atggtatcaa aaacaacgac caatgaaaaa
     6421 atgtctaagt accaatgttc atgattattg ggaaattgaa gtgacgagcg aaacaaggcg
     6481 cttacagtat gcgttccatg ttgttgggga agataacacg gactgttttt acggtgatca
     6541 aggaattttt ccttatcgag aaaatgtgat tactgaacca aatttttatt ttcgaatacc
     6601 ttattttcat caaatcgatc gattcactat tccagaatgg gtaaaagaaa cagtatggta
     6661 tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca aatgaccccg aacacacttt
     6721 accttgggga agtaaagatc cagaccgaga agacttcttc gggggagatt tgcaaggtgt
     6781 gagggatcat ttggattact tgacggacct tggcgtcaat ggcatctatt tttgtcccat
     6841 atttaaagcc acatccaatc ataaatacga tacgattgat tattatgagg tggatcccgc
     6901 ttttggcgat aaaaaacttt taaaaaactt gattgatgaa gcacataaaa gagggatacg
     6961 tatcatgttc gatgctgtat tcaatcatat gggtgtccat tcaccacagt ggcaagacgt
     7021 tctcaaaaac ggagaaaaat cgatctataa agactggttc cacatccgtt ttttcccagt
     7081 agattcttat cagatgactg acttaccaga aactgctgaa aatatcccat atgatacttt
     7141 tgcttttact ccattcatgc cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt
     7201 gctggatatc gcaacttatt ggataaaaga attcgatatt gatggatggc gtcttgatgt
     7261 tgctaatgaa gtcgatcatc atttttggaa aaaattcaga gaagcagtta cagaaatcaa
     7321 accagacatc tatatccttg gagagatttg gcattcctct caagcttggc tgcaagggga
     7381 tgaattccat gcagtgatga attatgcatt cacagattcg attaaagatt attttgcaaa
     7441 aaagaaaatc acagcttcac aaatggtcag tggaatgaat catcagcaaa tgctttacag
     7501 agatcaggtg aacgaaggaa cattcaactt attagattct catgatactg ccaggatctt
     7561 gaccctttgc caaggtaaca aagaattgat gaagtctgtg atggccttta tgtttcttca
     7621 aaaagggtct ccttgtatct attacgggac agaaataggg atgactggtg aagatgaccc
     7681 ggattgccgc aaatgtatga tatgggagaa ggaggaacaa gatctagagc tattcggttt
     7741 tataaaagaa cttgtttctc taaggaaaca gttgtctaag attatttctg aaggttctac
     7801 tcagtggcta atagtatatg atcgtgaaga taagctctat ttcacacggg aactagaggg
     7861 acagatcatc tatgtttact ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa
     7921 tgaggtgatt cttagtcaaa attgtcagtt gttagaagac gggaaagcag agatacgaca
     7981 atacggattt ttgatcatgt gttgaaaact gttaccggta acaacaagaa agcgtttgca
     8041 aatgaagatt tgtgcccatt ataataaggg tgtaacagca cgttgaatac acaggaggaa
     8101 atgaagatga aaaaaagaag tgtgaaacag ttgggtttag ggttaatctc tttaggaatc
     8161 ttaggcggat tagcggcttg tggcaatggt aactcttcag cagaagggga agccgatgaa
     8221 ggaaaaacgt taacagtttc cgttgattct ggatataaag attatataaa cgaaataaaa
     8281 aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag tagaaaagga catgttcgat
     8341 cagttggaat cattagcgtt agatgggcct gctggaaagg gacctgacgt tatgatggca
     8401 gcatatgacc ggatcggtgc gttaggacag caaggacatc tggcggaagt aaaactggga
     8461 aatgaagcag cttacgatga aacagacaaa gcccaagtga cttatgatgg aaaaatctac
     8521 ggagaacctg cagtaatcga gacccttgtt ttgtactata acaaagattt agtcgataca
     8581 gcaccatcaa cgtttaaaga tttggagaat ctatccaaag acagtcgttt tgcttttgag
     8641 tcagaagcag gaaaaaatac aggattttta gctaaatgga ctgactttta ttattcctat
     8701 ggtttaattg caggttacgg tggctatgta tttggtgatg atggcacaaa tccttcagat
     8761 atcggtttga ataatgcagg tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa
     8821 aatgtttggc caaagggaat gcaagacata aaaagtgcag gagacttcgc cagccagcaa
     8881 ttcatgtcca ataaaactgc agcaatcatt gacggtccat ggcaagcgca aacatacaaa
     8941 gaaaacaata tcaattatgg tgtagccaaa attcctactc taaataatgg acagccttat
     9001 caaccgtttg gcggaggaaa aggatgggta gtcagcaatt atgctaaaaa caaagacctc
     9061 tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg
     9121 gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag caaaggctaa aaacgatgaa
     9181 ttaacaacag ctgtgattga acaatatgaa acagctcaag cgatgccaaa catcccagaa
     9241 atgggcgaag tttggacagg tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa
     9301 acaccaaaag aatccgcaga tgaagcagta aaaacaatca gtgaagccat agaacaaaaa
     9361 tactgattaa aatgataacc gtccattttt ataaaaaatg gaacaggtgc ttcagataaa
     9421 agtggcaaga gcgagatgga aaattatctt gctcttgttg ccattatctt aaaagcagtt
     9481 tgcggtttag caagtttaga gggggcattc agatgcagca agataaaaca agcaatatca
     9541 acgaaaaaaa aatcacgctt tgttcactta ttccagggat ggggcagttt gtaaatggcc
     9601 aagcttttaa aggtatcgtt tttttcgcca tattcgtcct attcattggt cagatgcttc
     9661 tgggcggatt tcatgcattg gtaggattgg ttactttggg aagtatacca atggaagatc
     9721 attcattatt tattttgatt gaaggcactt tgcaattcat cattacaatc attttcttga
     9781 ttttttacgg tttgaatctt ttagacgctt atcgagtagc gaagatgcgt caagagggca
     9841 aaaaaatcaa tcggacgatg aaggaagtca tacataatat aggtgatgaa ggttttcctt
     9901 atctattgac acttcccgct tatcttctca tgatttttac gatcattttc ccagtcctag
     9961 ttacattgtt cactgcattt actaattatg acttcaatca tattccacca gccagtttaa
    10021 ttgattgggt aggggctaaa aactttttta gtatcttctt tttaagctca tatcgtaata
    10081 ctttttttgc agtcttttcg tggaccgtga tttggacttt atgtgcaaca actttgcaaa
    10141 tcacccttgg tattctcaca gcggttgttg ctcaccagaa atttattaaa ttcaaacgta
    10201 ttttcggtat cattttctta ttaccatggg cggtcccagc ttttattact atcatgagtt
    10261 tttcgaatat attcaacgat agcgtaggtg ccatcaatac tcaagtgatt cctttcataa
    10321 accagcttcc ttttgtagat atcagtgcaa tctcttggaa aacggacccg aactggacga
    10381 aagtagcaat tatcttagtg cagggctggt taggatttcc ctatatctat gtgatggtta
    10441 caggaatctt acaatcgatt tcagaagact tatatgaagc agcaaaaatc gacggagcaa
    10501 atgcatggca gcgcttttca aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa
    10561 catttattac acagtatacg gggaatttca ataatttctc catgatctat ctgtttaaca
    10621 atggtggtcc cggaagcgta ggcggaggag ctggttctac tgatattctt atttcctgga
    10681 tctataaact tacgactgga acttctccgc agtattctat ggcttcagca atcacgttga
    10741 ttatctccgt catcgttatc tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca
    10801 atatggggga ttaacaatga aaaaatttca ttcgagtgct cgattccaaa aacgactttc
    10861 tcactttttt acttactttt ttcttttaat actgtcagtc attatcatct atccgctttt
    10921 gatcacagca agtactgcct tcaagtcagg aaatattgca gcttttagtt tagatctttc
    10981 aagtgactgg actttaggaa actttactcg gctattcagt gaaacacttt acgggacttg
    11041 gtataaaaat acattgatca tttctgtcct aacaatgatc gtacaagtga ctgtagtaac
    11101 tttggcgggc tatacttata gtcgctatcg ttttatcggc cgcaaatcaa gtttgatgtt
    11161 ctttttagtg attcagatgg taccgactat ggcagcattg actgcctttt atgtcatggc
    11221 tttcttatta gaagcgctcg ataagtactg gttcttgaca atgatctata taggcggtgg
    11281 aattccaatg aatacttggc tgatgaaagg atactttgac actgtaccaa tagatttaga
    11341 tgaatctgct aaacttgatg gggcagggca tttcaggatt tttttccaga ttgtattacc
    11401 gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct tttatgggac cttttgggga
    11461 tttcatgtta gctaagttcc tgcttcgaac acctgaaaat cagactgttg ctgtcggctt
    11521 acaaacattt atttctgatg tgacaaacca aagagtctct ctttttgctg ctggggcaat
    11581 tttgattgca ctaccgattt gtctattatt cttcttttta caaaagaact ttgttaccgg
    11641 cctgacatct ggcggaacaa aaggatagga gcagagtatc atgcaaacat ttccgttaaa
    11701 ctatttttcc gcattacgta cgccaactca agtgtttgcg ggacgcaaac tattatcatg
    11761 gcctaaattt ttcttgatat ttgtattttt agtttccttg atggtcatgc ctgtgactct
    11821 tttttatgcc aatcagatcc aagcgattcc attagaacaa tttctatccg ttcattcgct
    11881 gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca
    11941 agcatcgcaa caaacgatag ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga
    12001 gaaacaacaa tccgatcatg gaacttttat tgattttgag aaggaacaat gggtgatcca
    12061 acaaaaggat aaatctggta tacgccgcta tacgatgaat tatagcccat ctttccaacc
    12121 agattctgta cgtacgcccg aagatttcca acgattttta gaaagagaat tttatgccag
    12181 taatcgtcca acaatcattt tatcttatag tttaagtctt ggtcttgtcc tatttgtcat
    12241 gacgagtctc attttattcg gggcttcctt tttcttgtgg atgacaagaa aaagccaact
    12301 ttcatccatt catacattca aagagagtgc aaatttgatg ctgaatgtta tgggaatagg
    12361 ttccatggca gcagcagttg tagggtttat ccactttgac tttatcttga tgctaagtgt
    12421 ccaaacaacg ataactgtct tgctgctttt atgggttttt gtcaaaacaa gattcaaaga
    12481 taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa
    12541 aaataaagta gtttttgaaa aatttctgaa aaaattaaac ttcttagttg attcttttct
    12601 tttttagtat taacgtaata gggtagtttt tttaaggagg aaaatcatgt tagagaaaac
    12661 tttatacaat caattatttt cacgttcttt ctcgctccct gtcgaagtaa attattggga
    12721 cggtacaacg aaacaatacg gagatacgga cactcctccc caaatcaaaa tcaatatcca
    12781 tgaagaaatc ccaatgaaag agattacaag taacgcttct ttggcactag gagaagccta
    12841 tatggatcat cgaatcgaga tcgagggaag tatccaagca ttgatcaacg atgcttatca
    12901 gcaagcaaac agctttttac gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa
    12961 acatacgaaa caaaaaaaca aagaagatat ccatgcgcat tatgacttag gtaacgactt
    13021 ttataaactt tggctagatc caactttgac ttattcttgt gcttatttca atacacctga
    13081 agatacgttg gaacaagcac aaatcaataa agtccatcat atcttagaca aactctttat
    13141 caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg ggaacattga tgttcactgc
    13201 taccaaagag tataatgtca aggcaactgg gatcacacta agtgaagaac aatacgattt
    13261 gattaccaaa agaataaaag aagaacattt agaagataaa tgccgtgtat tattaatgga
    13321 ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca agtgtaggta tgttcgaaca
    13381 tgtaggttct gaaaatcttg aaggttattt caaagtggta aaagatttat tgaaaccaaa
    13441 aggaacagct ttgatccatg gaatcagccg acaacaaggc ggtgcgacaa atgcttggat
    13501 caataaatat attttccctg gcggttatgt ccctggtgtt actgaattag ttgataatat
    13561 tacaaaaaac gatttgcaac tgattgattt ggaaagctta cgtcgtgact accaattgac
    13621 attagaacat tggacaaaga attttcatca agtccaagat caagtcacga aagaaatggg
    13681 tgattccttc tatcgaatgt gggatctata tttacaagct tgtgcagctt catttgaatc
    13741 aagtaatatc gatgtgattc aatatttgct tgttcaacct ggaaacaatg acatcccaat
    13801 gcatcgtgca taattgaggc aaaagtactg tttaaagact atttaaaaag aagctaagtt
    13861 ttatgatgct ccagaagtga acttttttgt ctaacttcta ggattgtctc taaagcttag
    13921 tttctttttc tttatatttt cataataaaa gtttggttct ttaattttta aacatgacag
    13981 aaaatttttt cttcgttata cttgtaatag aatactattt tataacaaag gggatcgaat
    14041 caatgaaaaa aactgttatt tatactagtt tatttgcagc tttgttttta gctgcttgtc
    14101 aatcaaatac caatcaaggc acatctggga atacgtcttc cagtagcgaa cagcgctctt
    14161 cacaggtatc ggactcctca acaaaagcaa gcacatcttc gtctagccag acaaaaagtt
    14221 ctagtgaagc aagtactcag tcagatatca aagtgtctgc agctcgtgcg atcgaattgt
    14281 tccaagataa atacccagat actgcaatta ctagtttgga attagatagt gattggggat
    14341 cttacttcta caagatagaa ggtgtcgatg atcaaaaaga atacgaagca agaattgatg
    14401 ctcaaaatga aaaaatggag acagagaatc cagaaactct agatcgagat gaacaaaatg
    14461 gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt gatcacgatt gaggaagcag
    14521 caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac agattgggat ttggataaag
    14581 atttaggtac tacttattgg gaagtcaaag taaaagacgg caatcaaagt acatcagtca
    14641 agatcaatag ccaaacaggc gaagtactgt caactgaaca agatgattaa gaatttcttc
    14701 ggaaaacaag agttgatttt tcttttagtc atgatacact agacaaaata acttgtttga
    14761 aggagcgatt tacttgagtt tagaaacgac ctttgccaat catatctttg ccaatccttt
    14821 gatgaatgca tctggtgtcc actgcatgac tacccaggaa ttagatgaac tggctcattc
    14881 agaagcagga gcatttatca ctaagagctg tacgatcaat gaacgaaaag gaaatcccga
    14941 accacgctat tttgatgttc ctttagggag tatcaattct atgggacttc caaacttagg
    15001 tttttcttat tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca
    15061 accgttattt ttttccattg ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa
    15121 aatagaaaaa agtgggttta atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc
    15181 tggaaaacca caactagctt acgattttga ggccacttat gaaacactga aagaagtatt
    15241 cagcatcttt tctaagccgc taggaatcaa attgcctcct tattttgatt ttgctcattt
    15301 tgatcagatg gctgatatct tgaatcagtt tccattgacc tatgtcaatg ctatcaacag
    15361 tgtgggcaat ggtttgtata tcgatacaga acaagaagca gtagtcatca aaccaaaaga
    15421 agggttcggc ggcattggcg gagagtatat caagccgact gctttggcga atgttcgcgc
    15481 tttttataca cgtttgaaac cagaaatcca aatcattggt acaggtggga ttcggacagg
    15541 acaagatgca tttgaacatc tgctatgtgg cgcaagcatg ttacagattg gtacagagct
    15601 tcataaagaa gggccggaaa ttttttctag aatcataaaa gaattaacgc agattatgtc
    15661 agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca tttcatagca
    15721 aagagacgaa ggtcatgatt gatcttcgtc tttttttata aaaaacactc gtctgttcat
    15781 tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt gggaaaataa cagaggcaaa
    15841 aaggaggata atatgaatga tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt
    15901 agttttcaag aactagttac ggatatacag aaaaaaataa agaagcaaaa tcatgaattg
    15961 aatgcgtttg tcgcgcttga accattcgag agtaattccc acgagctatt gattccccag
    16021 tcagaaaaag ggccattggc tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa
    16081 aagggatggc tagctacttc aggatctcgt ttatttgaaa cacaccgagc ttctcataca
    16141 tctaattatg tgcggcaagc agaagcgata ggtctagttc cttttggaca aaccaatgcg
    16201 ccagagtttg gtttcaaaaa tattacagac ccagtcatct atggcccagc aagaaaccct
    16261 tggaaccttg aatgtacacc gggcggatca agtggaggag ctgctgctgc agtttcatca
    16321 ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt ctattagaat acccgcttcg
    16381 ttttgcggat tgattgggct gaaaccttcg cgagggacga tgccagttgg tccatatgct
    16441 tggagaggat ggcaaggagc tgctattgat ttcggcttga cagtttctat gagagacacg
    16501 gaggctttat tttatgggat gagaagtatc cattccggtg cgccatatca agtatcgcca
    16561 gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga tagctttttg tgttgattca
    16621 ccgatccact cacagattag tgaagaggca gtacttgctg taaaaaaagc tgtagatttc
    16681 cttggaaaag aagggcatga tttgacagag attccttacc cactagatgg tcgtaaactg
    16741 atcacgtctt attatcagat gaatggcgca gaaacagcag caatgatccg ctcgattgaa
    16801 acaggcatcc agcgagctgt cagaaaagaa gaacttgaac ccatttcttg gactttgttc
    16861 caatatggtc agaagctctc tgccgctact tacatcgatt ctctgcatgt atgggatgaa
    16921 gcagcaattg tcatggaaaa cctacatcaa gaatacgatc tattcctttc accaacgact
    16981 gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg atgcgataag agcacagatg
    17041 gaacacatca gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc catgtttgag
    17101 gagagcttga agatcacacc ttatacacag ttggctaatc tgactggaca accagcaatc
    17161 agcttgccta ctcatttgac aaaagaaaac atgcctctag gaatccaatt tatggctgcc
    17221 agaggaagag aagatttgct atttcaaatc ggctatctat ttgaagcaaa tcatcagttc
    17281 catttaccaa atccatggaa atagcagaaa caaggagaag aagaatgaag aaaaaaagat
    17341 tgtttgctct aataactagt gcctttttta ttttaagcgc ttgcggtaca gctgccgcta
    17401 atcaggaaga taacagctgg agtaatgtag aaaaaaagaa agaattagta gtagcgactt
    17461 ctggcacact gtttcccgca tcttattata atgatcaaaa tgaattagtt ggttatgatg
    17521 tagatttggc aaaagaggtg gctaaacggt taaatgtcaa aatctccttt aaagaataca
    17581 acgtggatgg acaaatcact tctgtttcaa aaagagaatc agattttgct gcgaacgatt
    17641 ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc agagactttc aaatattcat
    17701 ttgacagtat gatcgtacga aaaaaagatg attcaggaat ccactcactt gaagatctta
    17761 aagggaaaaa agcagctggc gaacccaata ctagctatat gcgtttagcg gaaaaacacg
    17821 gagctaaatt agtcacatat gacaatgcca cgaatgatca gtatttaagc gatgtagcaa
    17881 atggtcgaac agatgttatt ctaaatgatt attatctgca gaaaatggcc gtatctgctt
    17941 taccagatat ccctgtaaaa atactggaga acgtttattt caatgaaaac gaaagtggtt
    18001 ttttattgaa agaaggaaat gatgctctga aaaagaaaat cgatggcgta ttaaaagaga
    18061 tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata ttttcaaact gatgtttcag
    18121 tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg atgtactttc ctctaattga
    18181 tttttccctc atgtggactt cacttccttt cgtactgcaa ggcttggtat acacattagg
    18241 gattggcttc gtgtcgtttc ttttaggaaa tttggtaggg ttgcttttga cagttttagg
    18301 tcttttggat tggctgcctc tgaatgtatt tattcgtttt tatttatcgt tctttagagg
    18361 cattcccgca ctagtcctgc tgtttctgtt atatttcggg ttaccttatc aattaagtgc
    18421 cttaacggct tcagtcatct gctttacgat taccagcagt gcatttattg gtgaaatcta
    18481 tcgaggatca ctagcaggcg tttcttcagg tcagtgggac gctggtttag cttctggttt
    18541 ttctttcttt ggggtattga aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc
    18601 ggcactcagt aacgtagcta tggatctagt gaaaggtact tcgttagcag caacaatcac
    18661 agtgccagaa atatttcaaa aagcaaagat cataggcggt cggacattcg attatttttc
    18721 tctctatatt ttagttgcac tgatttattg ggtcatttgt atcgggatcg aaaaagtgca
    18781 acagtattta gaaaaacgtt ctatccatca gtcaatctga aaaatagaaa tggtgctagc
    18841 gtggctggca ccatttctat tttttgtttc ttgtatattc tgcctagtat tttaaaacca
    18901 tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta
    18961 accatcgaaa tagaatcctc gttataccaa tattgcatcg agcaaggcgg cttggttgta
    19021 gaagaagtca cgatgcctga tgaccaagga attgtagata cgcttagctg cttgacaaaa
    19081 tttgatggaa gccgagaatg gcgatgctat gagctgaaag tgacaaaagc agattttcgc
    19141 tctacagcca aactgtcatt tgttggacat tataattatt ttgttttacc aagtaaacta
    19201 tatgaagaag taaaaactga aatccctaca ggaataggtg ttttagtgta tcgtccgtac
    19261 acggttgaag aggagatgcc tgcctctggt acatttctga ttgcgaaaaa agcagcaaag
    19321 aaagagcttg ctgtaccgga agaagctttg acaaaccgct tcatggcttc tttattcagg
    19381 gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat attttccaaa agatcaattg
    19441 tacaaagaat tgaaaagacg ttcagaaaat caagatccat tttctaacga acgatattat
    19501 gaacggtttt tagaggaaat agctagtcag gcaatcaatg agttgcaaga agaagttatc
    19561 gcgcttcagc atgattatga atatttgagg caacaacagc agatccgtcg gttgcctaca
    19621 gagccattgg agtgaaaaat atgtactatc cttatttaag aggaaaacaa tttgacctat
    19681 tagcattaaa agaagcactg tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg
    19741 aaccagtacg tgattccgct acgcttaaaa atgtgatcga attatttcaa aaaaagcacc
    19801 atccaatcgc tgtgatccaa aatccccaag taggacaatt caaattattc gatcagcatg
    19861 tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag
    19921 aaaatctcag tgaagtactg gattcacctc cagcgtttct tgttttcgat ggacagcact
    19981 acccaaaaga tacagaggtg tggaaacagt tggcaggagt tgattcgaaa tttttgatac
    20041 cagatactag tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc cgtgactctt
    20101 ttcaaacgag aaagcatgtt gaaagttatg cagataaaac agatgacttt tttagtgatg
    20161 actatctttt tttccacgca gacggatata ttggattttc tgatttcacg atcgaaggta
    20221 gccgttattt cgataaaggg ttccctagcc gagcgcttgc tatccatcta acgtatattg
    20281 atgcttacgg caatatacgc gtgaaacatt ttgtctctga cagtaacgac tctgctaaag
    20341 accaagcatt gaagtttctt gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc
    20401 atcatcaact cttgattact tcaggaatga ttgaattatt ggctctttac cagcaacaaa
    20461 aattccctgg actgggagtg ttgaaaaagt ggtcacttat gcatcatttg gaacttatta
    20521 gtcacctgct agatcatcca aaagattggc ttaaatatta ttcaaaagtt caggaactat
    20581 atgaagtgat ccaatgatta gataatgtaa atgaaaaagg aatagaaaag aggaaactgg
    20641 atgtatattc ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat cattaatgcc
    20701 gcagcggatt tcattgcttc gcaaggctct cctcaatggc aaaacgggca gaaaccaaca
    20761 aaagaaacga ttttgaacga tattgaaaga aaagaaagct atgtattaat agtggaagat
    20821 caaattgctg gaacagctgc attagtctca ggcgtggatc ccgtttacac aaaaatagat
    20881 ggtagttgga aagaatcaac tggtccttat ttatcgattc atcgtgtcgc tttagcagaa
    20941 agtactcgag gaaaaaaact agcaaaaccg ttcttagaac atttgttgac gattgcagcc
    21001 agcaaaaata tccaagatat ccgtatcgat acttatccag ctaatgaacc gatggaaaag
    21061 accatctatt ctgctggatt cacttatcaa gggatgattg aatttccttt tgaacacggc
    21121 gaaagaaaag catatcaaat aatcatcaat tgactataaa atcatctgag caaagataaa
    21181 gacattaaca ctactgaaaa aaacaatcat atgatagcca agaggttgta acagaagtgg
    21241 ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc tcaaattttt gtggaatttt
    21301 agcgtatttt acgaaatagc tgctttctga aactcccttc atcgagaggt cgtgacatag
    21361 cttttgtcac gatctctcgg cttatcctac gagtattatt cgatacattt atatcaataa
    21421 tccttttttt attttatctg cagcttcttg gaacgctcca tcgtttgaca cgactaaaag
    21481 tgaggggata gctttcaaac gtttcaactg tgctaaatct tctacataaa ccgccagtct
    21541 ggctgaaatc tgttcagatg aatctccacg ttttttcagg cgcgattgca atgtttcttt
    21601 agagatcgta ataaagatag gacggactac cgtcccgaaa tactcatata aatgccaaaa
    21661 accatttgct gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt taatttctgc
    21721 tttactggaa ccatatttat gttgagcata ttggtcccac tctgcaaact catttccctt
    21781 gatcatctga ttgaatgtat tgtcagaaac aaaataataa tcctcttgat ctttttcccc
    21841 aggtcttggt gatcgattcg tgtaagtaat gatcttttgt tccggctgaa aaatagcttc
    21901 tgcgagtgaa gtttttccag aaccagaagg accgataaat acatagcaat aattcattgt
    21961 catttctcct ttgatgattg aacaagtgaa aaagctttgg aaaatatttc caaagctttt
    22021 aaaagtaact ttgctttttt gtattttata ccagcaagcc agtcacaata gcacttaaaa
    22081 agctgactaa agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca agatttcctt
    22141 tttcttcatt taatcccttc atcgcaccag aaatgatccc gatagaagag aagttggcaa
    22201 atgaaacgag gaagacagat ataattgctg ttgtacgctc agtaaaatgg aaattaccat
    22261 ttgctaattc tgtcatagca acaaattcat ttgtaaccag tttagtcgcc atgatactac
    22321 cggcatccac tgcttctttc catgggattc cgctgatgaa ggcaagaggg gcaaagatgt
    22381 aacctaggat ctcttggaaa gtgatgccga atatcccgtg gaagatagca ttgatcatgg
    22441 caatcaaagc aacaaaaccg ataagcatag cagcaaccgt gatcgccaca tggaaaccat
    22501 caagaatgta ttctcccaac atttggaaaa atgtctgttc tttgttttct tcaatgatca
    22561 gctcatcttc tttttcttct aatacatagg gatttacaat tgaagccaga atataaccgc
    22621 caaataagtt caaaacaagg gcagtgatca catatttaga atcgatcagt gccatataag
    22681 atccgacgat cgacatagaa actgtagaca tagccgatac actcatggtg aacaaacgtt
    22741 tctcagatag gaagggtaat tcctttttta tggaaataaa aacttccgat tgtcctaaaa
    22801 tagcagaagc tactccatta taagattcca atttgcccat cccgttgatt ttacttaatc
    22861 ctagaccaac agctttcata aataatggaa gaatcttgat atagcgtaga ataccgataa
    22921 tagcagaaat aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca cttcctttat
    22981 ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga agatgatcaa
    23041 acatttttgc caatacagaa acaaccgctg taccaaaagt tgtacgtaat aatacgaaac
    23101 caagaacaag ctgtaaaacg aacatcacaa ttagtggttt caatttgatt gcttttttgt
    23161 tactgctgat caaaaaagca agccctaatg tgacaaataa accaataata cctaataaat
    23221 aactcaaata atcactccta ttccaaattc cgttttattt tacctgtttt gcaaataaaa
    23281 tactaatctt tagtgaaatt ttcattactt ttttagagtg aaacgcattc attttccatt
    23341 agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt aaagtctata
    23401 aaaaaacgaa actatcgatt aagctaatag tttcgttttc aacatttata agtaaacttt
    23461 ttctattggt aatatagatt ttcggaaggg tagacaggtg cgcaggtaat atcgattcct
    23521 agttttttca atgtttgttc atcattttta ttgaccatca cagtagagtg ggcttgtgct
    23581 ccctcaagtt cagctagttt atcataagca acttgagcga ttggattcgt tactgcactg
    23641 attgataaag cgatcaagac ttcattgatg cttagtgcat ccaaagagga atgaagtgtc
    23701 tttgatttca atccttgtat tgtttcaaga actaatggtg acaataaagg aatgtcatct
    23761 gcgatatggg ctaaatgttt tactgcattt aagataactg ccgctgacgc atccatcaat
    23821 gaacttgtac gcccggtaat gatgacttca tctggtaatt cgattgcaat cacagcagaa
    23881 ggatcttttg taggactttg cgcttgaatc ttatctgcat agtcatgggc agggactacc
    23941 acgtttcggt cttcaggacg taaaccgact tcttccataa tcagtttgat tcgattaact
    24001 gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg tttggaaata acgacggatg
    24061 atttcttgtt tagaggcttc ttggacaacg gtatcatcca cgatcccaaa tcctacacga
    24121 ttcacaccca tgtcagtagg agattgataa acagattctt taccagtaat tttttcaata
    24181 atacgtttga ttacaggaaa tgtctcaaca tcccgattat agttcacagc tactttattg
    24241 taggcatcaa agtggaagga atcgatcata ttgacatctt ttagatcgac tgtggctgct
    24301 tcataagcaa tgttcagcgg atgtttcaac ggaacattcc atactgggaa agtttcgaac
    24361 ttggagtaac cagctgcatg tcctttttgg ctttcatgat acagttggtt caagcaagta
    24421 gcgagctttc cacttcctgg gccaggagct gtcactacaa cgattggttt agttgtttca
    24481 atatactcat tttttccaaa accatcttct gagacgattt tttctacgtt gactggatag
    24541 tcttcgattt ctgagtgttt atagaccttg atgtttcgtc tttctagttt attgataaag
    24601 actttcgtgg caggctgtcc actatagcga gtgataacga cactgttgat taataagcca
    24661 taaccacgta attcatcgat taaccgcaag atatccatat cataagtaat cccataatca
    24721 ccacggatct tattacgttc aatatctcca gcatagacgc aaatcagtat ttcagcttga
    24781 tctttcaatt tttgcagcaa tttgattttc gcatcttctt caaatcctgg aagtacacgt
    24841 ttcgcatgct tgtcgtcaac taacttccca ccaaattcta aatataattt gtcatagtgg
    24901 ttcactcgtt ccaaaatata cgctgactgc tcttcaatat atttttctga atcaaaacca
    24961 atttttttca ctaggttccc tccttaagta ctaaagcatt ttttcatttt tttgaggatt
    25021 cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt
    25081 ctagatagtt attcattggt atgatagttt ttgataagga aggtgctaca tgaaaaaaag
    25141 acagaaaaag aaaaacgcat ataaacacta tatccgttcg atttttaccg gttatgaaag
    25201 aatgcttgaa gatcccgaat tggaacaatt gacgtttact tatctaaacg aagaaacgca
    25261 gctaactcgt gatgagcatc agcgcattca tttcacaaca agagatctgc ctagtaaata
    25321 aaaaataaga taaaggtcat cggattcgat tctgtcctat cagatggaaa gactagttga
    25381 tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt tatggagcat tgcccgctct
    25441 tttttataac aattttttac agaaaatggt actattgaag tattaataag tgaaaaacag
    25501 atgagtaaga ggaggcaagt agtatgaaag agccagatac aaatgcaaat tatgagccag
    25561 acgaactaga ggagacgaac gcggatcagg atacaggaca agaatcgtct tctttttacg
    25621 atatcaaaga aaaagcaagc atgaatcttt cccaattatt agaagactta caggcttttg
    25681 aaaaagctgt ccgcgaagaa gatatccctg agatctaccg tttatacaat ggccgattga
    25741 atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta
    25801 agatccatga tcgtttttta caggaatttc cttttatgga acaagtagaa aacatctcgc
    25861 caacgatgag ctattacaaa atcggcactt attaccatga tcggcccacg gtcggtattg
    25921 atgcaagtct gccagaaatt tttgttttgc ctaagatcga tgaagagtgg gagaaatatt
    25981 cccaaccgga gtcaacggac tatgacaaaa aaatcaatga gctagacgca aaggtgatca
    26041 ctgctcagac agaaattgaa cgtttagatg aacaaataaa agaaatcaat cgacaaatga
    26101 ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag
    26161 aattagagaa gaagaagcaa gtactatcta atgaaaaatt aggctggctt ccttatattg
    26221 agacgcctga aacaatccag cagcaaaaag aagcattgaa gcaagaagca agagcagacc
    26281 agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat cagacgctat ttcggtagca
    26341 aagagggatt cggtcaagca atccatgaat tcttgatgaa ttatcttggc aatgagaatc
    26401 ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga agcagaatgg
    26461 acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt ggaagaacaa
    26521 acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac aaaaatacat
    26581 gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca tgcttacgaa
    26641 ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga tatcgtcagt
    26701 ttgattgaaa cagcggaagc agaattagcg gatttacgta tgagaaaaga tttgatcgaa
    26761 tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga agaaccaatc
    26821 tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact gaaaccgttg
    26881 aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac aagacatttt
    26941 tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt attaccgacc
    27001 atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc agagacaatg
    27061 gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc agtggatcga
    27121 gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt attagcccat
    27181 gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa gccacttcgt
    27241 ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat ttttatcgtg
    27301 gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta tcaagtacga
    27361 ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac agtcttgtcg
    27421 atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta cgaattcttg
    27481 gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat gtcattcaga
    27541 taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa gtcttttctg
    27601 tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt aggaatgtcc
    27661 acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg taggatcgct
    27721 tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat agtactttac
    27781 ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag cagtttttat
    27841 tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt tctgagtttg
    27901 ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa gggagtctgt
    27961 ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac gctgttatat
    28021 cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt gatgcacagt
    28081 ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat
    28141 tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg tcagtatact
    28201 cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa aggaggaagt
    28261 aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa
    28321 agaagtggtc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa attttgaagt
    28381 caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg agaaaaatca
    28441 actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca aagagatcaa
    28501 attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt cataaaaaac
    28561 tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga aggaaaataa
    28621 catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag gggctaatat
    28681 tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa
    28741 cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat tcggtgataa
    28801 acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag caaaatactt
    28861 tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg gggtgatgga
    28921 agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca ttgttatagc
    28981 aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat
    29041 caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag
    29101 tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg tcttggcttt
    29161 agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga tcagcggatt
    29221 gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat tttatagtct
    29281 gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag cttttgaacg
    29341 tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa cggagatttt
    29401 agttgcagca aaagcagata tcgttggtaa caaagagaat gaagcttact cggtcatcaa
    29461 tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aagatgtata tatacattga
    29521 aacagataaa tatgacccaa attattctcg taaataaaac gtttattcat gaattgaagc
    29581 aaaaatgaga aaaatataat cttgttttta aataaaacgg ctacaattta gcgaatttat
    29641 gttacaatga gtccgctgtt tatttgttgt aattaaagga gatattttaa tgaacaaaaa
    29701 gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg ccactttgta taagctatat
    29761 accagtagga ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc
    29821 aggactgtta tcgattctgg tcttttccgg tggcgcgcag tttttagttg catctatgct
    29881 tacgacgcaa gcatcatttg caacgacatt attgatggtt ttctttttag aattacgcta
    29941 tacattatta ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc
    30001 tgtcttttct caatcactga atgatgaaaa ctatgcggtg aactacttaa agttttcaac
    30061 tgacccttcc tggaataaaa gaaaggcttt atatgtcaat tggttctcaa tgactgcatg
    30121 ggccggtagc aatatgatcg gtaatttttt cggctcggtg attcgagtag atgctgacct
    30181 tgtccatttt gctttgacgg ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc
    30241 actactgatc tttacaggtt tattctcagg cgtattaggg gtcgtattca tggtattatt
    30301 ccaaaatact ttcggattga ttgcagcaac agtcattgct tcatttgccg gatttatgct
    30361 ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa
    30421 tgagccgctc ttcaacttcc cggaacagga ggccaatcac catgactaaa ctatatttgc
    30481 tacttttagt tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt
    30541 attttactca tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat gtaccggtgg
    30601 cattatttgc ggcgttagct tttaaagatg ttttcattac tcatgaacat ctggatattg
    30661 cttggaatat caaaatagct gcaatgattc tagtagcagg cgtggcttac aaaacacgtt
    30721 cgatggcgtt gtcagttttg acaggacttg cgtctgtctt tttgctgtct atgctttaaa
    30781 ctgttttatt ttattaaaaa cagagattgc gacataagtt gttcaaactg atctatccga
    30841 actgcatttg caacagtcaa ttctggatgc aagtttattc gtagggataa aaaagattag
    30901 tgttggctta tgatgcaagc tcttttttat tttaggaatt agagagggat ttgtccttct
    30961 atgatttttt gctataatag ggaagatgtt tattttatga aagaatagtt gagggaattt
    31021 aaatgaataa aaaattgatg caaggaacat tttggctaac ctttgctaat cttctatgta
    31081 aagttttagg ggtagtctat ttgattccct ggctaagtat gatggggaac aatcaagatg
    31141 gtatgcttgc tacgacattg tataatgttg gctatcttcc ttatggattg ttcctgatgc
    31201 ttggtacagt cggatttcca aatgcgattg caaaaaaagt agctgtagcc acaaaagaag
    31261 gagataacgc tgcttgtcgg ctgattttta gaagtacgat caatatcatg tttgtcatcg
    31321 gtatcgcctc tgctgcactg atgtatttat ttgctccttt actagccgga atcagtccga
    31381 ttgctaacgt taataatggg atactagcta tcagaagttt atgcccatct ttgattgcta
    31441 tcccaattct tagtgcaatg agaggatatt tccaaggaaa aaatgatctg cggccttatg
    31501 gaacttcttt gattatcgaa caggctgtgc gagtgatcgt tatcttggct ggtacttatt
    31561 acttgcgcgt attgactgac ggaaccatat tagaagctgt attgatcagt acagtcgctt
    31621 ctttcttcgg tgggttagct gcgattatcc atatgtatat cgtcggacgc agaaaagact
    31681 tcttcgaact taaagatttt ttgatttcca gccgttattt cgaaagagaa aaccgatctg
    31741 cctctgtctc tatcattcgg gaaacactac cgtttatttt tgtcggctca gtcattacta
    31801 tcgttcaaat gatcgaccaa gtaacgatga aaccactgct tcactttttc cgtccagaga
    31861 tcgcagatca gcaactggaa ttcttattca gccgtgcttc tgtcaatcca aataaattga
    31921 cattgatcct gatctccatg gtaggaacag tagctataag cagcttgcca atattaagca
    31981 cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt aggagatagt ttttcgattg
    32041 cgttattgat tcttcttcct tcattgactg gaatgtcttt attagctgga ccactataca
    32101 cattgttttt tggctatgat cctgaaagtg tcggatattt ccaaatggct ttgctagctt
    32161 ctttgttttt ctctttattc acgatcctat ctacgatgct tcaatctttg aatcaccatc
    32221 gttttgcgat ccgattaact gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg
    32281 gattaggatt agttggcgga tacgggatga gcttatcaaa tggcttagca ttcggtattg
    32341 tctttgttcg cggctatcac tttatgtgca aagaataccg aatagcacct ttagcaaaga
    32401 tcagcaattt tttcttaaaa actttccgct ccaccctgat tatgcttgca gtatgttttg
    32461 ccgttttctt tttgctgaat cagcgattgt caatggaatc aaaaagctat gcaatgattt
    32521 attgtgtggc tgtaggaagt attggcggct tagtattttt attttctcaa ttcggaaaaa
    32581 atgggtttcg aatgttgaaa aatgttaaac atcatagcag aagcaaagaa taggttaact
    32641 aatcgttttt tgctgtcact tgaaaaacgg caactcgtat gattacgctt gtcgtttttc
    32701 tattttccaa ttttagtttc atatctaatc ttacttatga tatagttata aggtctggaa
    32761 aaattctttg aaacgaggaa attaaacaca tgaaacttgc agtagttaca gatagttcgg
    32821 catatctgcc ggaacgtatc aagaaccatc ccgacttatt cgttattcca atccctttga
    32881 ttatggatgg gaaaatttat aacgaaggaa tcgatatcga agcggatgaa tattatggac
    32941 ttttaaaaaa cagtaaagat tttcctacta cttcacaacc tgtcgtaggg gaagttttgg
    33001 gtttatatga ggaattgaaa gagaaagggt atgacacgat tttaagtatc catctttctt
    33061 cagggatttc tggctttgtc aacacattat ttgcgatgaa agatgacatc agcggagtaa
    33121 cggtcattcc ttatgactct aagatcacta gtatgccgat gggacacatg gtagaagctg
    33181 ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa
    33241 tccgagataa tacctatgct tatctgattg tagatgattt gaacaattta gtacgaggag
    33301 gacgtttgac aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga
    33361 cattttctga agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg
    33421 ctcgagctga agaaattatt ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt
    33481 acgtgataca tgcaaataat ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa
    33541 aatatccaaa tgcagaaatc gaaattggtc atttcggacc agtcattggt acacatttag
    33601 gtgaaaaagc catcggcatt gccatttcag cgcaataatt ccttttattc aaaaaacata
    33661 ccaaaaatat
//
LOCUS       DAXWLF010000011        32914 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXWLF010000011 DAXWLF010000000
VERSION     DAXWLF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.011"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            75..350
                     /locus_tag="ACGE4U_000387"
     CDS             75..350
                     /locus_tag="ACGE4U_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFF8392578.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            356..1123
                     /locus_tag="ACGE4U_000388"
     CDS             356..1123
                     /locus_tag="ACGE4U_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFF8392579.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1291..3120
                     /gene="typA"
                     /locus_tag="ACGE4U_000389"
     CDS             1291..3120
                     /gene="typA"
                     /locus_tag="ACGE4U_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFF8392580.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3381..5153
                     /gene="recQ"
                     /locus_tag="ACGE4U_000390"
     CDS             3381..5153
                     /gene="recQ"
                     /locus_tag="ACGE4U_000390"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFF8392581.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5267..5569
                     /locus_tag="ACGE4U_000391"
     CDS             5267..5569
                     /locus_tag="ACGE4U_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFF8392582.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5572..6735
                     /locus_tag="ACGE4U_000392"
     CDS             5572..6735
                     /locus_tag="ACGE4U_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8392583.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6761..10189
                     /locus_tag="ACGE4U_000393"
     CDS             6761..10189
                     /locus_tag="ACGE4U_000393"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFF8392584.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10210..11334
                     /locus_tag="ACGE4U_000394"
     CDS             10210..11334
                     /locus_tag="ACGE4U_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFF8392585.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11315..11761
                     /locus_tag="ACGE4U_000395"
     CDS             11315..11761
                     /locus_tag="ACGE4U_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8392586.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11745..12083
                     /locus_tag="ACGE4U_000396"
     CDS             11745..12083
                     /locus_tag="ACGE4U_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFF8392587.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12202..12762
                     /gene="rsmD"
                     /locus_tag="ACGE4U_000397"
     CDS             12202..12762
                     /gene="rsmD"
                     /locus_tag="ACGE4U_000397"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFF8392588.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTIDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12759..13250
                     /gene="coaD"
                     /locus_tag="ACGE4U_000398"
     CDS             12759..13250
                     /gene="coaD"
                     /locus_tag="ACGE4U_000398"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFF8392589.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13240..14283
                     /locus_tag="ACGE4U_000399"
     CDS             13240..14283
                     /locus_tag="ACGE4U_000399"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFF8392590.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14442..15170
                     /locus_tag="ACGE4U_000400"
     CDS             14442..15170
                     /locus_tag="ACGE4U_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFF8392591.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15286..15531
                     /locus_tag="ACGE4U_000401"
     CDS             15286..15531
                     /locus_tag="ACGE4U_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8392592.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15528..15746
                     /locus_tag="ACGE4U_000402"
     CDS             15528..15746
                     /locus_tag="ACGE4U_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFF8392593.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15882..16421
                     /locus_tag="ACGE4U_000403"
     CDS             15882..16421
                     /locus_tag="ACGE4U_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFF8392594.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16414..18687
                     /locus_tag="ACGE4U_000404"
     CDS             16414..18687
                     /locus_tag="ACGE4U_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFF8392595.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18725..19771
                     /gene="holA"
                     /locus_tag="ACGE4U_000405"
     CDS             18725..19771
                     /gene="holA"
                     /locus_tag="ACGE4U_000405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFF8392596.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19860..20114)
                     /gene="rpsT"
                     /locus_tag="ACGE4U_000406"
     CDS             complement(19860..20114)
                     /gene="rpsT"
                     /locus_tag="ACGE4U_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFF8392597.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20514..20783)
                     /locus_tag="ACGE4U_000407"
     CDS             complement(20514..20783)
                     /locus_tag="ACGE4U_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392598.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21045..21173)
                     /locus_tag="ACGE4U_000408"
     CDS             complement(21045..21173)
                     /locus_tag="ACGE4U_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392599.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(21253..21708)
                     /locus_tag="ACGE4U_000409"
     CDS             complement(21253..21708)
                     /locus_tag="ACGE4U_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFF8392600.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21846..23774)
                     /locus_tag="ACGE4U_000410"
     CDS             complement(21846..23774)
                     /locus_tag="ACGE4U_000410"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8392601.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(23971..24804)
                     /locus_tag="ACGE4U_000411"
     CDS             complement(23971..24804)
                     /locus_tag="ACGE4U_000411"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFF8392602.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(24996..25700)
                     /locus_tag="ACGE4U_000412"
     CDS             complement(24996..25700)
                     /locus_tag="ACGE4U_000412"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8392603.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(25723..26349)
                     /locus_tag="ACGE4U_000413"
     CDS             complement(25723..26349)
                     /locus_tag="ACGE4U_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8392604.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            26675..29050
                     /locus_tag="ACGE4U_000414"
     CDS             26675..29050
                     /locus_tag="ACGE4U_000414"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFF8392605.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(29074..29565)
                     /locus_tag="ACGE4U_000415"
     CDS             complement(29074..29565)
                     /locus_tag="ACGE4U_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFF8392606.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            29752..30672
                     /locus_tag="ACGE4U_000416"
     CDS             29752..30672
                     /locus_tag="ACGE4U_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8392607.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            30826..31170
                     /locus_tag="ACGE4U_000417"
     CDS             30826..31170
                     /locus_tag="ACGE4U_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFF8392608.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(31360..31860)
                     /locus_tag="ACGE4U_000418"
     CDS             complement(31360..31860)
                     /locus_tag="ACGE4U_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8392609.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            32388..32471
                     /locus_tag="ACGE4U_000419"
     tRNA            32388..32471
                     /locus_tag="ACGE4U_000419"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32422..32424,aa:Leu,seq:cag)
     gene            complement(32795..>32914)
                     /locus_tag="ACGE4U_000420"
     CDS             complement(32795..>32914)
                     /locus_tag="ACGE4U_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFF8392610.1"
                     /translation="YLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        10929 a         5704 c         6690 g         9591 t
ORIGIN      
        1 aatatgacaa cttcttggtt tattaccact tcctatgcta cactgaagag gaatatgaaa
       61 taggagagtt gaagatgcaa gaatatcagt atccacttga tttagattgg acaacagacg
      121 aaatggtcat tgtaatgaag atgtgggaag atctggaacg tgcgaatgaa aaagggataa
      181 acaatgaagc ttttttgaag acttatcagc agtttaaaac tgttgtcaaa tccattggtg
      241 aggaaaaacg attgggacgg gaatttgaaa aagcttccgg ttattcttta tatcgtacag
      301 tgcagcaagc aaaaaaaaat ccaaacaaaa ctttgagaat gaacgggtga gataaatgga
      361 acagatgatt gatcatatca agtcctggct tgaagaagct ggtgagaaaa tcaaatcttc
      421 tcaacaaaca ttaacaatct cgcaaaaatc aaatcgaacg gatctagtaa caaatgtcga
      481 tcgaaacatc cagaattttc taatcgaaaa aatccattcc tttgatccag aggcgaaaat
      541 attggcagaa gaagaagggt gtaatcaatt atcggatctc gatggaagag tctttatcat
      601 cgatcctatt gatggcacct tgaattttgt gattgaaggg gaaaattttt gtattatgct
      661 cgctgtttat gaatccggcg ttggaaagct tggtttcatt tatgacgtga tgagaaatga
      721 acttttttgg ggtggaaaag gaataggcgt gtataaaaat gatgagccat tgcctagacc
      781 agcaaataaa tccttatcgg aaggcttact tggtgtgaat agttatcttt tcggtcataa
      841 taaatatgga atccgagaaa ttgcggaaag cagcatgggg acacgtatgt acggatgcgc
      901 tggtcttgaa ctagtggcaa tcttgaaagg aaaccacatt gcttatattt caaaccttag
      961 cccttgggat tatgcagcgg gaaatgtctt attggaagag tttggtatgg taacaagcgg
     1021 tctttttggt actcctttga catttgaaaa aagggaatac tatttagcag ggacttcttg
     1081 tgcatatgca gaaatcgcag accttttgaa aaaagaaggc tgaaaaaaag gtagaaatca
     1141 ttcacaaaaa tgataaaaac gagcaaaaga ttaattttct cgaaaaaatg aaaacatttt
     1201 actatatttc atgaaaaaag tctgctataa ttagcagtag agtaaaattt gccctgtcat
     1261 aaagaaccaa taaaaaagga gttagttaca ttgaattata gaaacgatat ccgtaacgtc
     1321 gcaattattg cccacgttga ccacggaaaa acaacattag ttgatgaact attaaaacaa
     1381 tcagatacat tagacgcaca tacgcaattg caagaacgtg cgatggactc aaatgcgcta
     1441 gaaaaagaac gtggtatcac gattttagcc aaaaacacag ctgtagacta taaaggtatt
     1501 cgcgtaaaca tcatggatac tccaggacac gcggacttcg gtggcgaagt agaacggatc
     1561 atgaaaatgg ttgacggcgt tgtgttggtc gtagatgcgt atgaaggaac aatgcctcaa
     1621 acacgttttg tattgaaaaa agcactggaa caacatatca caccaatcgt tgtcgtaaac
     1681 aagattgaca aaccttctgc tcgtcctgag catgtcgtag atgaagttct tgaactattc
     1741 atcgaattag gagcagatga tgatcagtta gactttcctg tgatttatgc atctgcacta
     1801 aatggtacgt caagcttgtc agatgatcca gcagatcaag aaccaaccat ggcacctatt
     1861 tttgatacaa ttattgaaaa aatccctgct cctgtcgata acagcgacga accattacaa
     1921 ttccaagttt cattacttga ctacaacgat tatgtaggtc gtatcggaat cggacgtgta
     1981 tttagaggaa caatcaaagt aggagaccaa gtagcattaa ttaaattaga cggaacagtt
     2041 aagaaattcc gtgtaacgaa attatttggt ttctttggac taaaacgtct agagattcaa
     2101 gaagcaaaag ctggagactt gattgctgtc tcaggtatgg aagatatctt tgtcggtgaa
     2161 acagttactc ctgtggatca tcaagatgcg ttacctatcc ttcacattga cgaaccaaca
     2221 ttgcaaatga ctttcttagt taataattca ccatttgccg gtcgagaagg taaattcgta
     2281 accgctcgta agattgaaga acgtttgatg gcagaacttc aaacagatgt atccttacga
     2341 gttgaaccaa caaactctcc agatgcatgg acagtttctg gtcgtggaga attgcatttg
     2401 tctatcttga ttgaaaacat gcgccgtgag ggatacgaat tgcaagtatc tcgtccagaa
     2461 gttattgaaa aagaaatcga cggtgtaaaa tgcgaaccgt ttgaacgcgt gcaaatcgat
     2521 acaccagaag aatatatggg tagtgttatc gagtccttga gcttacgtaa aggtgaaatg
     2581 caagatatgg tccatacagg aaacggacaa atccgtttga cattcttaac acctgcccgc
     2641 ggtttgattg gttattcaac tgaattcctt tcaatgacac gtggttacgg aatcatgaac
     2701 catacttttg accaatatct gccaatgctt cctggtcaaa ttggcggacg tcaccaaggg
     2761 gcgcttgttt cgatcgatac aggaaaagca acgacttatt ctatcatgag tatcgaagaa
     2821 cgcggtacag tcttcgtaga accaggtaca gaagtatatg aaggaatgat tatcggggaa
     2881 aacagccgtg ataatgattt gactgtcaac atcacgaaag caaaacaaat gacaaacgta
     2941 cggtctgcta ctaaagacca aacatctgtc atcaaaaaac caaaacaatt gacgttggaa
     3001 gaatctttgg aattcttgaa tgatgatgaa tattgcgaag taacaccaga aagcattcgc
     3061 ttgagaaaac aaattttaga gaaaaatgct cgtgaaaaag catctaaaaa gaaaaaataa
     3121 tctgtaaaag gatgaagaga gaaaccagct tgttataaaa aatagctggt ttcttttttt
     3181 gtccaactct tttctgtgga gaagtcatct ttgtcaatgg acataaattc agaaaatacg
     3241 ccgaaagacg tttaaaagac ttttaaaaaa cttatttcta gagagttttt gtttcataat
     3301 aagataatgt ttcacgaaag gagatcaact tgtccttcga agaaaatcga agtaaaatgg
     3361 caagagaaag gagcgggaag atgaacgact tattaaaaca atactttggt tatgatgagt
     3421 ttagacctgg tcaaaaagaa attatccaga aagtcattga tcaggaaaat gtattaggaa
     3481 tcatgcctac cggaagtggg aaatcaatct gttaccagct tccagcttta ctattagatg
     3541 ggcttactgt ggttgtgtcc cctttaatct ctttgatgaa ggatcaggtg gacgcggcga
     3601 atcaattagg tatacccgcc acttttatca atagctcgtt agatggttac gaaacagcta
     3661 gacgcttcca agaaatcgat agacagcagt accgtttact ttatatcgct cctgaacgtt
     3721 ttatcatgcc ggattttatc caagcaatga agcgatggaa cgtccgtatg atcgctattg
     3781 acgaggcaca ttgtatctct cagtgggggc atgatttccg cccaagttat ttacagatgg
     3841 cgaaccagct agatcaactg ccaaatcgtc ctgtaattgt tgctttgaca gctacagcga
     3901 ctgtgcaagt agctgctgac ataaaaagac ttttaaaaat tccagaaaac aaccatattc
     3961 agactggttt tgaacgagaa aatctacgat ttcaagtaat taaagatcaa aaaaaagaac
     4021 aatatctaat cgaatattta aagatcaaca aaaatcaatc tggtatcatt tatgcagcaa
     4081 cgagaaaaga agtagaccgt ctctatcatt tgcttaaaaa attcgatttt tccgtgggta
     4141 gatatcatgg tggtctaaat gagaatgagc gaacagaaat gcaagaagcc tttttatacg
     4201 atcgcttgca gctgattgta gcgacaaatg catttggtat gggtataaat aaaagcaatg
     4261 tccgttttgt tatccattat caaattcctg gctctctaga agcatactat caggaagctg
     4321 gtcgggcggg aagagatggt ctttccagtg aagccatcct gcttttctct cctcaagata
     4381 tccaagtcca aaagtttttt atccagcagt cccaaagaga agaagggcaa aagcaaaaag
     4441 aatatgaaaa attaaaagcg atgacagaat acgtgtatat tgagtcctgt cttcagcaat
     4501 acatcctaaa ctattttggc gagacaagca gtccgtgcaa ccgttgtggt aactgtttag
     4561 atgacagaga aatagtagaa gtaacgacag aagcacagat ggttctttct tgcttaaaac
     4621 gtatgggtga gaactatggt aaacaaatgc tgatgaaagt cttagcagga tcgaaagaac
     4681 aaaagctgca agctttaggt tttggacatt tatctaccta tggactgatg aaaaaccaat
     4741 cgcaaaaaga aacgatgcag ttaattgaat atttgatatc gaatggctat cttctgacca
     4801 tcaatggtga gtatcctgtt ttgaaagtaa cagaaagagg gatacaagta ttgaaaggac
     4861 aagaatctgt ttatcgaaag gaacccaaga aagtccagca gctatcggat gaagaaaccg
     4921 atacactatt tgaagtgctg cgggaactgc gaacggatct agcatcagaa gcaggcgttc
     4981 ccccgtatgt cgtattttca gattccacat tgaaagaaat gagccgaata cgtccatcaa
     5041 gcagattgga gatgcttcag ataaaaggag ttggccaaag caagcttgat aaatatggcg
     5101 aagccttttt atctcggata aaaaatgcgg actctaacgt tctggataag tagaaagaac
     5161 gaatacgtaa aaatgaataa aaacaactgg ttaaatctgt tttttttgct ccttatgaaa
     5221 atcaaatatg ttataattaa ggaattagaa tgggaggtaa gcacagatgg attctttgtc
     5281 gaaaaaactt gcgatccaag tattaaatga tgaagcagaa cggatcaaac gattaatccg
     5341 aaatcaaaac aacagtctct gtatctcaca atgcaaagcc ttcgaagaag tggtagatac
     5401 acagatgtac ggcttttccc ggcaagtcgg ttttgctgtc cgtataggag taatcggaaa
     5461 gaacgagggc cagctgatgt taagtgagct agaacgagaa ttgaatcagt tatatagtga
     5521 ggtttaccaa gaaatttatg ataaaaaaga agttggcaag gaggaataga cttgcctaag
     5581 aaagtgaaaa aaaggcatct attagactat agcatcttga ttccctattt aattttatgt
     5641 gtattgggat tgatcatggt atatagttct acgtcgtatc tattattaga aaacggacaa
     5701 aatccgtccg catcagtcat taaccagagt attttttggg tgcttagctt gattgctatc
     5761 gcactacttt ataaaatgaa aacagatgtt ttgaaaaacc agcgtttgat catggcagcc
     5821 atcgcagttt tgacgatttt gctgctgatt gttgtgtttt ttggtaagga gatcaatggg
     5881 gcgaaaggtt ggctgcaaat tgcaggattc tcgatccaac ctgccgaata cctaaaaatc
     5941 atttctatct ggtacctgtc tctgacttta tctaaaagac aaaacagtgt acaaaaagat
     6001 tttcttggga ctgtgaaacg tccacttgca atggttatcg gacttactgc attagtcgct
     6061 attcttcccg attttggtaa tgcggcagtt atctttttga ttattttggt tctgctttta
     6121 gccagcggag tcaattatgt gtatactttg attgtaggag taggaggctt ttgtctaagt
     6181 acttttacga tctggctgat caatatcaca aacggaaaga tcttacccgg acgtttacag
     6241 tatatctata atcgatttgc tatttaccaa aatcctttct cagacgaatt aaataaaggg
     6301 catcaactag tcaacggcta ctatgccatg ttcaacggag gactatttgg caggggatta
     6361 ggtaacagta tccagaaaaa aggattttta caagaagcac aaaccgattt tatttatgca
     6421 atcgtagtgg aagaactggg agtgattatg gggattttga tattagcgtt gctattcttt
     6481 atgattgtgc gcattgtcct tgtaggtatc cgttcaaaag atccgttcaa ttcgcttttg
     6541 tgcattggga tcggcgcaat gtttttgatc caagtatttg tcaatcttgg aggaattact
     6601 ggcgttattc ctttgacagg gatcacgttc ccatttttaa gtcaaggagg ctccagtttg
     6661 ctgatgcttt cgatttgtgt cggctttgtc ctcaacatca gtgcggatga aaaacgcaag
     6721 agcttaggtt tataataaaa aaagatgtag gagtggttcg atgaaaaaag ttttagttgc
     6781 taacaggggt gaaatcgctg tacgtgtatt tcgtgcttgt acagaattag gaattaaaac
     6841 tgttggaatc tatgcagaag aagatgaata ttccgtacac cgtttcaaag cggatgaagc
     6901 atatttagta ggacaaggaa aaaaaccgat tgatgcgtat ctagatattg aagggatcat
     6961 ttctatcgcg aaagaatgcg gagctgacgc tattcatccg ggttacggtc ttttgtcgga
     7021 aaacttgaat tttgcgcaac ggtgcgaaga agaaggcatc atttttgttg gacccaaact
     7081 gcatcattta gatatttttg gagataaaat caaagcaaaa gctgcagcta tcgaagcggg
     7141 gattgcttcg attccaggca cagatggacc aatcgcttct atagacgatg ccttggagtt
     7201 tgcgaaacaa tacggttatc cgattatgat caaggcagct ctaggcggcg gcggacgcgg
     7261 catgcgtgta gctcacgatg aaaagagtgc aagagagggc tacgaacgtg cgaaaagcga
     7321 agcaaaagca gcctttggta gtgatgaagt gtatgttgaa aaatacatcg ctaatcctaa
     7381 gcatatcgaa gtacagattc tcggggatac gcatggaaac gtcatccatc tttttgaaag
     7441 agattgttct gttcagcgcc gtcaccaaaa agtagtagaa gtagcgcctt gtgtctctat
     7501 gaatgaacag caacgtcaaa aaatctgcca ggcagctgtc caattgatga aatatgtcgg
     7561 gtatgtcaac gcaggtacag tagaatttct tgttgaagga gatgattttt actttatcga
     7621 agtcaaccct cgcgtccaag tggaacatac gatcacagaa atgattacag atattgatat
     7681 cgtaacaacg caactgttga ttgctcaagg tttggatctg cataaagaaa tcggattgcc
     7741 tcagcaagaa ggaattaaat taaatggttc agccattcaa tgccgaatca ctacagaaga
     7801 cccgttgaat aactttttac cagacacagg gaaaatcgat acgtatcgtt cacctggtgg
     7861 ttttggcgta cgtctggatg tcggaaatgc ttacgcgggc tatgtagtga caccgtattt
     7921 tgattcattg ctagtaaaag tatgtacgca tggtgctacc tttgaaacag ccattcagaa
     7981 aatggaacgt tgcttgaaag aatttagaat ccgtggagtg aaaacaaaca ttccatttat
     8041 gctcaatgtc atcacacatc ctgaattcca atcaggaaat gcaaaaacaa ccttcattga
     8101 tagtacagca acattatttg aatttcctcg actacgagac cgcggaaaca aaacgatgaa
     8161 atatatcggt gaaattactg tgaatggttt tccaggtatc gaaagtggcg aaaaaccatt
     8221 ttatgaagaa ccacgtatgc ctaaagatct aatcacaaga actgattacg taacggctaa
     8281 gaatgtatta gatgcgaagg gtgccgatgc tttggtcgaa tggatcaaag gacaggaaaa
     8341 tttgttgttg acggatacga catttagaga cgcccatcaa agtctgctag caactcgtgt
     8401 tcggacaaag gatttcaagc agattgcacg tttgactgga gaaggtcttc ctgaactctt
     8461 ttcaagtgag atgtgggggg gagcaacatt tgatgtggct taccgctttt tgaatgaaga
     8521 tccttggcaa cggttgagaa aaattcgttc tttaatgcct aatacgttgc tgcaaatgct
     8581 tttcagaggt tcaaatgccg ttgggtattc taattatcct gacaatgtat tggtggaatt
     8641 tgtcaaagaa gcagcagcgc aaggaatcga tgtatttcga atctttgata gtttgaactg
     8701 gactccacaa atggaaaaaa gtatccaagc tgtacgggat actggtaaaa tcgcagaggc
     8761 agctatttgc tatactggcg atatcaacga tccaagtcgg gcaaaataca atgttcagta
     8821 ttataaagac atggcaaagg aactagaaca actgggtgcg catatcattg caatcaaaga
     8881 tatggctggg ctgcttaaac cgcaggcagc ttatcgtttg attagtgaat tgaaagaaac
     8941 gacagatctg ccaatccatc tgcatactca tgataccagc gggaatggaa tcatcactta
     9001 ttctgctgct tcaaaagcag gagtagacat cgttgacgtg gcaatgagtg caatgagtgg
     9061 gaatactagt cagcctagca tgagcagctt gtattatgca ctggtcaatg gtccacggct
     9121 gccagaaatc acgattgaaa atgcacagaa attaaaccat tattgggaag atgtgcgcat
     9181 gtattacaaa ccgtttgaaa acggcttgaa tgctcctgaa acagaagtat atatgcatga
     9241 aatgccagga ggacagtatt cgaatctaca gcaacaggca aaagctgtcg gattaggtca
     9301 tcgctgggat gaaatcaaac aaatgtatca tactgtcaac ttgatgtttg gcgatatcgt
     9361 taaagtaact ccatcatcta aagtagttgg agatatggca ctatttatgg tccaaaatga
     9421 tcttacagaa gaagatattt atgaaaaagg cgaaacacta agtttccctg aatctgttgt
     9481 gactttcttc caaggagaac tagggcaacc cgttggcgga tttcctaaaa aattgcaaaa
     9541 gattattctt aaaggcagac ctgcattgaa tgaacgtcca ggtcttttgg ctgaatcagt
     9601 cgacttcaac gaagtaaaaa aagaattggc agaaaaaatt ggctatgaac caaaacaaga
     9661 cgaagtatta agctatttga tgtatccgca agtctttttg gactatcaaa aagcatataa
     9721 tcaatttggc gatgttacat tactggacac accaacattt ttccaaggta tacgtttggg
     9781 tgaaacaatc aatgtacaaa tcgaacgcgg aaaaacgctt atcattcgtt tagatgaaat
     9841 cggagaaccc gatatcgaag ggaatcgcgt actgttcttc aatttgaatg gacaaagacg
     9901 tgaaatcact atcaaagata attcgatcat cagtacggtc caaacccgat tgaaagctga
     9961 accgacaaac cgagaacaaa ttggagcaac gatgtctggt tctgttttgg atgtattagt
    10021 gaaaaaaggc gataacgtta aaaaaggtga tacgttgatg attactgaag cgatgaaaat
    10081 ggaaacggcc atcgaagcac gttttgacgg agaagtcgca catgtatatg tgtcttcagg
    10141 agacactatt tcgtctggtg accttttgat tgaagtgact gagaaataat aagcgaaagg
    10201 tggggaaaca tgaaaagact gggtttgttt tggctgattc tgctattagt tattgtggca
    10261 ttctacatgg aaccagtctt gtttccgcca aaacttgaag tgaaggaaaa ctctgatcaa
    10321 caaatatcaa accaaatgac aacgtggaaa taccaggagc ttaaagcaac tggtttttct
    10381 acttatatcg gaaaaccgat cgatcagctg gaaaaggatt ttggccaagc ctatgatcga
    10441 ttaactagcg gatttggttt tgaaacaaga tattatgaaa ataaagctag tcatctcaca
    10501 tttgaagcga acatccaaga cagcagagtc acaactgtta aagtactgca aacagggcct
    10561 gctgatatcg cgccgtttta tttagggatg acgatgcaag atcttacgaa aatcactact
    10621 atctatacta atttcacttt tgagtaccat gatacagaag ttggaatcga actaatggaa
    10681 gaggatatga attatcgtcc gctaattgct tttgataacg acacttttgc tattttattt
    10741 tttgatcaaa caacaaacgg cctattttca gttgtgtatt tagataaaga tagtcttttg
    10801 aaactattgc catatcaagt gtttggtgaa ggccttccac attatcaagc agataaatcg
    10861 gctgattggg aatcaatcaa tcgcgttaaa gaacgaaaaa gtacgacttt attaaatatg
    10921 cttagaagag aagacgaact tcccatatac aatcaaacac ttgcctttca aaaccacagt
    10981 cagctgctac tacatgattt tttgacaagg cctgaggaaa tattgtcaga ggaacgtatg
    11041 gctgattggc aaaaatcgct aggcagtgct caaacgacag tgaaattctc actgacgaat
    11101 gatgaattgg agaagttaat cagtaaacaa aaattagaaa atactagtgg gctatttatt
    11161 catccggcga ttgacgctac tttttcgttt ttatattggt acagcgatcc ttacaaccat
    11221 gaccgtttca tggacccggg aacggattct gtaggcattg ctttttctaa agaaaatatg
    11281 ttagtcttat tgcaagaaga aaacggagaa agtagtgata gcagtgatcg ttaacgaaga
    11341 gctgtttcaa ctggaggatc aatgcctcca agtcgctgaa atgataaaaa agagtaaaac
    11401 gatggcttct tatcttgaac ataaacagtc aatggaagaa tctgcaaaag tttgccagtt
    11461 gaaagaagcg ttcttgtcta agaaagaagc atttgaaaat gtagcggcat atggtgagta
    11521 tgcacctgat tatcgtgaaa aaaaacgagc tgttcgaaaa gcaaaaagac agcttgatat
    11581 ggatgagcat gttgcagctt tccgatatag tgaaacacag ctgcaaagtc tgttggatca
    11641 aattgtccaa gtacttgccg aagaagtttc taatgaaatc aaagtagatg caggtagccc
    11701 gttttttgaa acgaaaaaac actttggatg cggaggtaat tgtcatgcaa ttagacaata
    11761 gtgaatttga aataacaaaa cgacgcgggc tgatcgtatg ggtctacagc ttaaaacaat
    11821 taaagaatct gaaaaagttc ggcttgttgc attatgtctc aagaagaatg aaatatgtcg
    11881 tgctttactt aaatgaagag acattggaag aaacagaaga acgaatccgt aaattacatt
    11941 ttgttcgaaa cgtagaacgt tcttaccgac cggatatcga aatgaatttt gccgaaaaaa
    12001 taggaaacaa aacagtatta aaagacgaag gtttcgaaat tgaagagcta aatacagaaa
    12061 tccgattagc tgaacatctt taaaaatctc ttatgagcag tttttaaata aaaaataact
    12121 tggataacga tcgatttttg atcattgtcc aagtattttt ttggtatact agtaccagta
    12181 ccatttctaa ggagtgagta gatgcggata atatcaggaa attatggcgg gcgccgttta
    12241 aaaagtttag caggggcgaa caccagacca actatagata aagtaaaaga atccatattc
    12301 aatatgattg gaccatattt tgatggagag acagtattgg atctgtttgc aggaagcggt
    12361 ggtttagcta tcgaagcagt atctagaggc tgcagtcatg cggtatgcgt agacaaaaat
    12421 taccaagcgc taaaaatcat caaagaaaac atcgaaatta cgaaagaacc agagaaattt
    12481 accacaataa aacttgatgc ggacaaagca atcgaacggc tagcagcgga gaaacaacaa
    12541 ttcgactact tgtttcttga tccgccatat gcaaaacaaa aaataatcga tcaaatccat
    12601 aaaatggaag aactgggact tttttctaaa accgcagtta tcgtatgcga aacggataaa
    12661 acagtcgaat tgccagaaac tgtggctgga ttccatcaaa taagaaaaca gatttatggc
    12721 ataacggccg taactatcta tagaaaagag ggaaatcaat gaaacgtgca ttattcccag
    12781 gaagttttga tccttttaca aaaggtcact tagatacggt ggaacgtgcg gctaaattat
    12841 tcgatgaggt agtaattggt gtttttatca atacttcaaa gaaaagtttg tttcccccag
    12901 aagaaagaat gacattgatc acaaaagctg tttctcatct tcctaatgta aaagtcatgc
    12961 atcaagaaaa tcaattgacg gtagaaacag caaaagagat tggtgcagat gcgttgatca
    13021 gaggcatacg aagtatcaaa gactttgaat atgaacgaga gattgctcag atgaatcatc
    13081 atctatatga tgaattggaa actgtcttct tattagcaaa accagaatat agtcacgtga
    13141 gttccagtat tttaaaagaa gtactgcact ttggcggaga tgtgagcagc tacttaccgc
    13201 cagtgatcaa cgaagcttta gctggaaagc gtgaatcaaa tgaaagctaa acaatggttg
    13261 aaaacagtac tgattatcat atttggaatg ttgatcattg gatttgtagt agttcccttg
    13321 ccgtattatg tagaatcacc gggagccacg atcgatctga aagaactaat tacggtcaat
    13381 gataaggaag ataaaggttc tgggtctttc tctcttacct ctgttggtat caggcaagct
    13441 acaggtttct tagctttaaa agctaaaatg accccttttg aagaattgat cagtgaagaa
    13501 gaattgactg gtggggcgac aaatgaagaa tataatcaaa tgcaacagta ttatatggat
    13561 tcttcccaaa atgcagcaat cgaacaagcg ttaaaacttg cagacaagcc ttataaaatg
    13621 gaattcaaag gcgtatatgt catgagtgta gagaaaacat cgaatttctt tggcaagctt
    13681 tcagttggag atacagtaac aaaagtagac gggaaaagct ttcaaagtac agaagcattt
    13741 atggattatg taaaaagcca aaaagttgga caaacaatcg aaatcagtta tttgcataat
    13801 ggtgaagaaa aaacagcttc tggtgatctg atcgagttac caacagataa aaaggcagga
    13861 ataggtatca cgcttactga ccataccgag atcgaatcag atacagatgt tcgtattaat
    13921 tctggctcta tcggtgggcc atcagcggga ttgatgttca ctttagaaat ctatgaacaa
    13981 gtcactggta aaaacttgcg acatggtaag caaattgcag gaactggaac gatcaacagt
    14041 gaaggagaag ttggccgaat cggaggaatc gataaaaaag tggcaagtgc ggataaagct
    14101 ggcgtagaga ttttctttgc accagatgat acgatttcag acgatgttaa gaaagaatat
    14161 cctgaaataa aaaccaacta cgaagaagca aaagctgccg ctaaaaaatt gaattcctca
    14221 atgaaaatcg taccagtaaa aacagtacaa gaagcgcttg actatctaga gaaaatgaaa
    14281 tgaaactaaa taattaaaga ggggttgtga cacgagttct gtcacaaccc ctctttttcg
    14341 tcaaaaatct tcacgataaa aataaaaaca aaagaaaaaa ttgacgtaat tagatgttga
    14401 agggacgatt ttgccagagc tcactgtttc ttcaatttta aatgaaagga gtaatgaatg
    14461 atttttactg gtcattcttt gaaaaaagaa caatgataga aaaaatcagc aagaaaatac
    14521 tagcttcacc caaattgatc atttggatgt ctatgatagt tcttatcttt tgtatcgcaa
    14581 gtggtttttt attatcaaat ttgctatttt atagagaaac agaagagact gctgcaaaag
    14641 aaatactttc ttcgtttagt tccagtggag aagatccatc agctgaagga aacgatgtag
    14701 aagaggatgc tcgtccaaag gtcatgtaca ccgatatcaa aggaagtgtg aaggagcctg
    14761 gtatctattc cttttcttca gaagaacggg tctatgatgt actaaagcgt gcaggtggat
    14821 tattagaaga agcggatagc gatcgaatca acttttctgc gaagatagaa gatcagcaag
    14881 tcctatatat ccctgctgta ggtgaagagc tgccagaaca tcttgatcag ttggcatcct
    14941 cggaaggaaa acagcctaca gcagattccg agcctcccaa aataaacatc aatacagcca
    15001 ctccatcaga acttcagcag attcctggga tcggatcagt caaagcgcaa gaaatcattc
    15061 gatttagaga agaaaacgga tccttccaga aagtggaaga cctgcaggag atttctggta
    15121 tcggtgaaaa gacagttgaa aaactaaaaa acttcgttac aattaaatag aatgattcaa
    15181 aatcgactgg cacgagcaat aaatggacgc taatggatac taagaataat ccaattctaa
    15241 gagcttggca ggacgaggag gtagtatttt tggataggaa atagcttgat tgttcgcaaa
    15301 gaaacattaa aaaaaccaat gctgaatgtc tatctccaaa ataaaatatc tgggattcat
    15361 attatgaata cggctgtcag cggcaataac agtcaagctc tacgagaacg ctttgccaaa
    15421 gatgtactta gctatacagc agataaagta tttattctca ttgggacgaa tgatctagca
    15481 gagcataaac aactttctaa agaaacgtac caaaaaatct gcagtggatg atagttcagc
    15541 tgaaaaaaga ctatagttct gatcatgctt tctttatcac gccaccagca gtcgatgaga
    15601 caaaacaaaa atatcggaat aacaagttga tcaaagaata tatctctgtt ctcacagaag
    15661 tagcaaaacg ccatgattgt gaagtatcga tttttatacg gaatttcttg aacaaatgga
    15721 ttcgaagaat ttttataatg ggctaatgga tgacggactt catttcggca gtgaagggta
    15781 ggatcttttg tcagacagga tgattcgtta tatttaaaga atattttgtt taagcaatga
    15841 gtacttttta atcaacttga aaaaacggag gaatataaaa catgttagaa aaatcttcaa
    15901 aagcatgcga tacgaataaa gaaaaagtta ctgataagtc acataatgaa cgcattcctt
    15961 gggaccagta ctttatggct caggcagtac tgttatcttt acgtagtacg tgtacacgtt
    16021 tagaagtagg agcgacactc gttcgagaaa aaaggatcat tgctggcggc tataatggag
    16081 cagtcagtgg agatgtccac tgcatagatg aagggtgtta cgtcgtggat ggacactgct
    16141 tgcgaacgat ccatgcagag atgaatgcac tcctacagtg tgcaaaactt ggtatcccga
    16201 cggaaggttc agaaatctat gtcacacatt ttccatgttt ggcctgtaca aaggctttgt
    16261 tgcaagctgg tgtgaaaggg atcaattatc tccatgacta tcgaaatgat ccttatgccc
    16321 aagcgttgat cgaacaatta ggtgtatctg tacaccaagt tgctttagac tcacagcatt
    16381 tttcaaaact tcaaaatgaa ttaacaggaa aacatgtcta gcgttttaga aaggcatgtt
    16441 cgaaatcggt tgatttttcc ggcaattacg tcgatgatta tctgtttgtt gtattacact
    16501 gacaattggg gttatcgttt gcttcttgcc ggaatttttt tatggttttc gctaaaagaa
    16561 ggacgtttta tcgcgtactt gtgtattttg tgtagcataa cggtatccat ttcttatagg
    16621 ctacaagagt attcattttt agaggaaaaa acaacatctg cgcaaattac tgtatttaca
    16681 gatacgattc gtgcaaatgg taattttttg acaatggaag gaaggttatc agagaataaa
    16741 caaaagctcc agctttctta tcaagtaaac acagaagcag aattgaaaac atggcttaac
    16801 ttccgaggaa agggaataga attgactgct gaaggcgagt tcatctctcc agcaaaaaag
    16861 cggaatcttt ataattttga ccaagaagct tacttcagga accatagaat ttctggacgg
    16921 ttccaaattc aaaagattga acagatcacc atgcaaaatc actggcgaaa ctggcttcag
    16981 agaaaaagag gatcttttat ctcctttgtc caatctgaat ttccgcaaaa aacaagtgta
    17041 tatattaatt ctttattatg gggctataaa gatgcaggat tcagagaacc agaagaaatt
    17101 tttagggaga gtggtttgct gcatctgttc agtctatcgg gtctacacat ccaattttat
    17161 ttagggtggc tgtattattt atttcgacgt atcggatggc cactacacgt cagtattatt
    17221 ccgttgagtc tactaactat ctgttatgtt tctattgcag gaagttctat ttctgttttg
    17281 cgtgctagtt tgctgttttt attgcgattg ctagtcaaat tgctgaataa gcattattcc
    17341 accatggact tatttgcagt tgtcttttgg cttcttttac tatttgatcc tcgatttctt
    17401 tttcatgcgg gtggacagct tagttttttc atgagttttt tgtttatcct gatttctttt
    17461 gatggagatt ggttgaaaaa aacggcctct cacgtcctgt ttactatgat cacgatgccg
    17521 ttgattgtat ggcattttta tgagtggcct attttaggta gtctttttac attactgatt
    17581 gcaccggtat tcaagtttct ttttcttcct ggtgtgttat ttctcacact cttcaatcgc
    17641 tggctgccgg aaactttact tttgctgata gaagagggat taatcttatt tgagcgaata
    17701 atcgattttt cttcagggtc gtctctcatt ataggacgtt tgccccttgt gctatctggg
    17761 atgtatattt tcggattact gatttgtatg gataggttga aagaacaacc tgtgaaacac
    17821 ttgttttatg ttggtctttt ttcttttatt tttttgactt tgccattttt acgcttttct
    17881 tcaacgatcg cttttatcga tgtcggtcaa ggagacagta tctttttgca aagtcctttt
    17941 cgaaaagaaa cgattttgat cgatactgga ggtcagttac actttgaaag agaaaagtgg
    18001 gcgcaaggta tgatacgtcc tcctgctgaa agaaatatcg ttccatatct aaaaggtcag
    18061 gggataagtg taatcgataa actcatcttg acacacgacg atacggatca tgtgggagaa
    18121 ttacccacgt tatctcagca ttttactatc aaaaaaattt atatcggttg gggcgctgga
    18181 cgaaatgagc cattacaaag gcatttatct gaagctcaaa aaaatggtac tgagatcatt
    18241 gaaatcaaac atggggacag gatcagcggt tattatgatc tttatgtatt gactcctttt
    18301 gaaaagggag agggaagaaa tgaagattcg attggtctat ggatggaata taacaatagg
    18361 cgctttttat ttttaggaga tttgaatcaa gcaatggaaa aacaattgct tttgatttat
    18421 cctaatctga aagccgatgt agtcaaatta ggtcatcacg gtagccgtac gtcaagtaac
    18481 acagattttc tttcccatat tgaagcaaaa catgggatta tatcctgcgg cgtaaataat
    18541 cgttatggtc acccgcacag cgaagtattg gagacactag aagaaaatca gataaaaaca
    18601 tggcgaacgg atcaacaagg aatgatcacc tatcgctggc atccagtttt tcatcctcat
    18661 gggctagtgg aaacaatgat agactagtaa ttctcatctg ctcatggtag gataattttt
    18721 gaaagtggtg agtaatttga gtatacaagc agagttacaa aaaatacgta aagaaaaact
    18781 tgcatcttgt tacttgattt taggaacaga aaagtttctc caagatcaag ttcggacaga
    18841 aattgtcaaa aaaatccaat tagagggcga agatgactta aattttttaa gttttgacat
    18901 ggaaaatgca agtttagatg aggtagtagc tgaagcagag acgttgccgt ttttcggaga
    18961 tcaacggtta gtattcgtag agaatcctta ttttttaaca ggtgaaaaag taaacaatgg
    19021 aatcgaacaa aatactgatt tattgacggc ttatttgaaa gaaccattag aatcgactgt
    19081 attagtattc tttgcgccat atgaaaagtt ggatgaacgg aaaaaagtaa cgaaacaatt
    19141 gaaaaaaaca gcaattatca tcaatgtcca acagttgaat gaaaaagaag tacgtcaata
    19201 tctgatgaat accttagaga attcctcaat caaaatggaa cgaagtgcaa ttgatttatt
    19261 cttgaggttg accgacttag atttatccaa aatgatgaga gagttacaga aattggtcct
    19321 ttatggacaa aatcaacagc aaatcacggt aagagaagta gaagaattag ttcctaaaac
    19381 gttagaacat aatatttttg acatgaccca atatatttta aaagggaaga cggaacaagc
    19441 tttgcgttta tacgaagatt tagttatgca aggggaagaa acaatcaaaa ttaatgcgat
    19501 tttgctttct caattacgtt tatttttaca gacaaaattt cttgtaaaga ttggttatca
    19561 gcaggcaaat attgctgaaa cattgaaaat ccatccatat cgtgtaaaac ttgccatgca
    19621 ggaagtaaaa aaattcgatg aacatttgct ggtacagttg tttgatcaat tagttgagat
    19681 ggattatcaa atcaaaacgg gacaaatcga aaaagaatta agttttcaac tattcgtttt
    19741 gcgcacaggg cagttgttaa gaaatcgata agaagaaaga cgagggcaaa gcatgcggga
    19801 aggtcgtttg tttttgtcct ttttttgaca aataaaaaaa tcggccgaag ccgattcttt
    19861 tatttagcga tttttttgct taaacgagat ttgtcgcggt tagctttgtt tttgtggatt
    19921 agaccttttg attcagccat atcaatagct ttaacagctt ctttatataa cgcatctacg
    19981 ttgtcagcac ctgaagctac agcatcttca aattttttga ttgcagtacg catagcgctt
    20041 gtttgagatg aatttttcac attagcgttt tcgcttgtac gaacacgttt gattgcagat
    20101 tcaatgtttg gcattagttt cacctccgat aacttaagtt cttacgcgtg ttaccgcata
    20161 attcaacatt cattattata cctaaaggca gcgtgctttg caatatgaac atgacaagtt
    20221 tttttcctta acagtctttt attaggattt tttttcagta aaaacggata gtacaagctg
    20281 aaaaaaatca aaaagacgct attgtgggag gcaatagcgt caaaccaaac tagattttta
    20341 tttatctatt taaacaaagt ttgaatggga atgtttggta ctaagttatt atataacaaa
    20401 gagaaccgtt tgcaaagttt tttttgaaaa gggatacgat tccctgttgt tctttgtttt
    20461 tctattaaga aactgcagtt attttataac cagtatcctt tgatcagtaa aggctattct
    20521 ggtgtaccga ttattttaat gtattcatcg atatttttta tcaacatttt ttttatgacc
    20581 aactggttcc gacggttcca tttgccactt acttccagca catatttatt taggggcaaa
    20641 cgtaagatat attttgccaa atagcgatct ttaagcaggc cattgatcac acggtcctca
    20701 agctgcaagt tgatcccagt cagcatcatt ggctgttctt gtaaaacgta cgtatcaatt
    20761 aaaagacctt caaaagtttc cacaagcgtc ttcctttctt ttcaaagtaa cctgaaaaca
    20821 ggcactaatt cttccatttt gatattcgtt gaagtggatg cgatcattct ttcttttgtc
    20881 cactgtaatc tagtataaca aaaaagagca gaagagccaa aaagaaaaaa gagcatcgaa
    20941 ttctatagga agtctcattg gcacggccta aattattaca aaacattgat taaaaaaagc
    21001 ccctgcatcg caggagcatc ccctatatcg ttttaatagg tcaatcaaaa agcagtatca
    21061 aacacatttt gtatcaaatc aggaaataat agataagcta agcccagaat caaaagtacc
    21121 gctagaagaa accagccgat tgttcctatg aaatacttta atttttcgaa cattgtatca
    21181 cctcgttgtc agtatatcac ggatcagtaa attgggggtg ttaagaatct cctaaaatag
    21241 acaaaatttg ttctattttc ctaaagcaaa tacttgtaag atacagtaag tgataccaga
    21301 agcaatgacc ggtccggcgg cgattcctct taagaagaca acacctaaaa tcgtgccgaa
    21361 aacaagtgca acagtgattt gtggatcaga agcaatcaat cctacaccct tggaagataa
    21421 gacagccact agcccaccgc aaaaaacagc tacccagcca ataggggatt taaaggcttc
    21481 taatagatca cgtagaccga tttctcctgt tgcgataggg attagaatag ccgctgtgat
    21541 gatcgtcacc ccaatattga ttcctttgcc ttgaagccaa gggaaaaagg aacgggaggc
    21601 tggtatgagc ttcataatca aaacaaaagc tgccgctatc atcagacttt gatttttagc
    21661 taaaattgct acaagtaaaa taattcctaa aaaaagccaa ctttccatga aatatattcc
    21721 tcactttctt cgaaacaatc ataacatgtt tttagaaagt tacaaaagta ggaattcatc
    21781 cttcaagacc tattcataag ctgttttttt cataaaataa acaaaatcat aaaaaaacac
    21841 tttgcttatt tgacagaaga cagccgcgct ttgtatagtg gacaagactt acattttttt
    21901 gaagaatcag aaagatcagg taaaggtctt agtaaacgca agccattcag aataaccaat
    21961 attgtgcttc cttcgtgtcc tacaactcca agaggtagat ttaaaaattg caggaagtta
    22021 gaagcaatca acaagagaat aacaagaatc gaaaaaataa tattttgttt gatgatccgc
    22081 cgcattttta aagatagccg atgactgtag acaaatttat ttaagtcgct tttcattaag
    22141 acgatgtctg cgatatccat agcggcatct gtcccttgcc ccatagcgac acctaaagaa
    22201 gcggctgcta aagcaggcgc atcattgact ccatcaccaa ccatcgcatt tacttggtaa
    22261 agttcattct cttttttgac tagttgtgtt ttttcttccg gagtacagct tgcatagtaa
    22321 tgttcaatcc ctacttgttc agcaatggcc tttgctgtgc cagaatggtc gccggttatc
    22381 atacttgtat ggatgttttg ggatttaaag taggaaatta cttgttgggt atttgctttt
    22441 ggaatatcta gcagacctaa aacagcaatt aactgctgat ctttagaaag ataaatcacg
    22501 gttttgcctt gattttccaa acgttcgatc atttccgcaa tctctttgct gattatcatc
    22561 gtctcaggat catatgcgtg cttgccaact ttccattgcg cacctaaata ggtagtttgt
    22621 aatccaaaac cagtaatttc ttctattggt aagtattgga tttcttcagg tattgtgcaa
    22681 tcgaaatatt gcaaaatcgc atgagcaagt ggatgcgtcg ttttttgttc catagcaaca
    22741 agaagttgtt gtgccaaatc cttttcatca aagaaaaagc tgtcagttac aactggtttt
    22801 cctttagtga gcgttcctgt tttatcaaag gcaatcgctt ttaattcaga aagcttttct
    22861 aaatggatac cgcctttgat taaaatccca ttttttgcag aatttgaaag agcagctaga
    22921 gaagctggtg tagcagatgc gacaagcgca caaggagaag ccacaactaa taataccatt
    22981 cctcgataaa agctttcatc ccaactccaa cctaggaaaa agtgggggag aaggatcatt
    23041 agaggaacag caagcaaaat caattttaca taattatttt cgattcgttc gataaatgaa
    23101 gctgtttgag ttggcgtatt ctgcgcttct tctactaatt ggataatctt agcaaagaca
    23161 gtttcggaac tcgtttttgt tactcgaatc gtgatgggat ttccttgatt cagcgttcct
    23221 gcatacatct catcgcctaa atatttttct acaggcacag attcaccgct taatgcagac
    23281 tcatcgatcg ttgcctgtgt gctttctaaa aagccatcta tcggaacagc tgctccttta
    23341 gcgacaaata ccaagttatc cacctttagt tgagaaacat ctacttcttg catttgtcca
    23401 ttttctttta ataattgggc tttttgtggt tgaagattca tcaaagcagt aatttctttt
    23461 tgacttttat tagttgtgta ttcctctaat gcaccgctta aacaaaagat gaatgtcaac
    23521 attgctcctt caaagtaatt accgatcaaa caggcaccaa tagcagctaa agccatcaat
    23581 aaatccacat tcagtgtccg atcattccac aattctttca agccttccga tgtttgttta
    23641 tatccaccga tgataatggc actgatataa aaaagtgggg tgatggtaag agagaatcgg
    23701 tcgaagataa aaccgaagat cataaataaa aggcagccga ttgttgctgc aaaagctttt
    23761 ttctgattag tcatcgttat tagacctcct tgtcaaaaca aggatagcat aataaaaatg
    23821 taattgataa taataacagc taaatgagaa cgataatggt tatcatttgg tgattaagct
    23881 tgatgttgtt tttattattt ataatgatta ttattaaaga aaataaaaaa ccagaacatt
    23941 tctcaaataa gagaagtgtt ctggtttttt ctacattgca ataaaccaat aaccaatcaa
    24001 aatgatacca aggataatac gataccagcc aaaagcggta aagtcgtttt tcttcaaata
    24061 gcttagtaag aatttgatag ctaagacgga aaccacaaaa gatacgaatg ttcctacaag
    24121 aagaacaaca gtttcttcca ttttgaatga attcccttca gctaaatatt tgacgatctt
    24181 caagccgctt gcgccaaaca tgacaggaat ccctaagaaa aatgaaaact ctgtagcgac
    24241 aaaacgcgaa gcaccaatca agatagatcc taggatcgtt gcgccagaac gggatgttcc
    24301 gggaacaagt gccaagactt ggaataaccc aacaagaaga gctgcttggt acgtaaagtc
    24361 attcagagaa gtccaacgag gtgttttgtt tttgttgcgt ttttcgacaa taataaaggc
    24421 aataccgtaa acgatcaaaa caatggcaac tggtaagaac ttgtggaaat ttgcttctaa
    24481 ccaatcatcc aaaggcaaac caatgatgat ggctggtatg caagcaacga tcactttaaa
    24541 ccaaagtgtc cacgtatctt ttttttctac cgttgattta cttggagaaa atgggtttaa
    24601 cttgtgaaaa taaagaacga cgacagccaa gatcgcccct aattggataa cgacattgaa
    24661 catcgacata aattgtgaac tggcatccag tttgatgaat tcatctgcta ggatcaaatg
    24721 accagtacta ctgatgggca gccattccgt tattccttca ataataccga gaatcactgc
    24781 tttgataata tttgcaaaaa acataaattc cttccttccc aattaagata catcttatag
    24841 tataccgtta ttttttgaga aacccaatct gttttttaat attaagaaaa acttgagaaa
    24901 taaagcagat aagtacacga agagaaaaaa gaagacaaag ccctcgttca gactttgcct
    24961 tttatgattc tctggtaaaa attaaaatat ccccattata gaaacgagtc gcgactatgt
    25021 gttttcccgt tatcgatata agacgtatca ttgatgccgt ggatagaaaa tcgaccgttt
    25081 gcataagtga cctttgttac gctgccattt aaaagcccag cacgaacagg ctgtttagga
    25141 tcgatcagat taagcaaaaa ggcgatcgtc aaaccatgag atacgactaa aacatttcca
    25201 ccaccatttt tttcgcagtg atgggcgata tcttcaaatc ctgtccatac acggtctcgc
    25261 aatgtttcat aagtttccgc ccacccagcg gtatctaagt tgtatacagc attagcaatt
    25321 tgttcaaaag aaacttctgt ggtaaacatt tcatcatatg ttttaaaatt caatacacga
    25381 gggatcacac cccacatttc catgtcatat ccaccttcaa gagacccgaa acaccattct
    25441 ctaatccttt tttcaataaa ataaggaatt tcttttccct tagaatgttc tcctagaata
    25501 atacgggcag tttcgattgc acgaccactg tcactagaat aagcaaatga aaagtcgaca
    25561 ttttttaacc cgcgacctaa ataatgaatg ccttcttgtc cttgctttgt gaggggtgta
    25621 tcacaccagc cttgtactcg ttcaattgta ttgaacatcg tttttccgtg tcgtacgata
    25681 taaagtgtag tttctgtcat tttattctcc tcctaagtcg ttttaacgat tgaaaaaagg
    25741 atttgtcctt ttttcctgtt caatcgtcgt tgcatcgcca tgtcctggat agactgcaaa
    25801 ttcatcaggt aatacaaaca gctgggtccg aatactatga agtaattgtt ctaaattccc
    25861 tgtatacaga tcggttctac cgatacttcc tctaaacagg gcgtcgccag aaacgacaaa
    25921 atcatcaaaa ataaagctga cgcttcctat agaatgccct ggggtaggga cgacgcgaaa
    25981 agacatgttt cctaaagtat aagttttcat ttcaaattca tattctgcag gcgagacaat
    26041 aacatcagca atatcatcat gtctaccgag tccagaaaga ttcaaaatcg gatcacctag
    26101 ccatttttgc tctagtgggc tgacataaac tgggatttga taatacgcgc gtaaaggttc
    26161 cactgcaccg atatgatcgt agtgagtatg ggtaagcaaa atcgctaccg gctgttggtt
    26221 tgtttttttg atttcttgtt tgattcgttc cgcatcttct ccagggtcaa tgatcaaaag
    26281 tgcttcttcg ttgtagacca aataacagtt ttcttcaatc attcctgttt ttaatctttc
    26341 gatatgcatg tgcattcctc cttgatagcc aaaacttctt tcatccatta gtataacgca
    26401 ttcattgcta ggaaacaaaa acaggtactt atttctcttt acttatctga attatcagac
    26461 tatatgaaaa gtatttggat attctaaaga gaaggaaagt aagttttcat agcattttaa
    26521 tatgtccttg ttacattaga ttcatccaaa aatccaaatt ctatatccta tatccctgcc
    26581 taatcagcag ggtgtttttt tatgtttgga aagagagctc tttttgctaa actaggtttt
    26641 ttcaggtaat cttagagaga aagaggtggt ggaaatgatc aaacagtcaa aaatatctgt
    26701 gcggcactta gtagaattta ttttgcgacg tggaagtatc gacaatcgta aaaaaagtaa
    26761 ccatactgca ttagaaggag cgagaatcca ccgcaagctc caaaaagatg cgggagaaaa
    26821 ctatgaaaaa gaagtctttt taaaaacaac agttcaatta gatgaatatt acctgatcgt
    26881 tgaaggccgt gcagacggta ttttcaaaag agacggtatt tattatatag atgaaatcaa
    26941 aacctccgaa ccacgatttg aagatttaga accagaacaa gtggaacttt tctttcatca
    27001 agcaagggtg tatgcctata tctatagtca agaacatgat ttacaagaaa taaatctgca
    27061 attgacttac ttccagacgg cagaagaagt gatcacgaga aaggttgaac atcagacaag
    27121 agagcagtta gaaacttttt ttaaaaagct gactgaagat tataaagaat ggcttgtttt
    27181 tcaagaaaac tggcgcactg tacgcaatgc ctctttgatg gctttgaaat tcccccatga
    27241 tgagtatagg aaaggtcagc gtgaactagc ggtagctgct tataaaacga tccgtacgaa
    27301 acagaaattg tttgtagaag cacctaccgg aacaggaaaa acaatctcta cgttattccc
    27361 cgctttgaaa gctgtagggg aagaagaggg agaaaagatc ttttatctaa cggctaaaac
    27421 aataactaga caagtagctg aagatgctat gacagcatta aaagatacag gagctgaagt
    27481 aaaaagtgtc actttgacgg cgaaagacaa gatctgcttt ttagatgaga cgacctgtaa
    27541 tccagatcag tgcccatatg ccaatggtta ttacaatcgg attaatgaag gtttatggga
    27601 tcttttgaat catgagaatc agatcactcg tgaagtcatc gaaacttatg caagaaagca
    27661 tacactttgt cctttcgaac tatcattaga tgtcagtatc tggtgtgacg taatcattgg
    27721 ggattacaat tatttatttg atccaactgt ttatttaaga cgtttttttg aagatgaaaa
    27781 aaacgaggac tatctgtttt tgattgacga agcacataat ttggtgaatc gttcccgaga
    27841 gatgtattcg gcagaactat cttatgaaaa aacgaaacga agtaaggaag ctataccaaa
    27901 ggaattcaaa aaacttcatc gccgtttcaa caaactatta aaagaatttg attcgatccg
    27961 agaaatagca aaagaagatc attgggatta tcatcatcaa aaagcaccgg ctgaatcttt
    28021 agtaaaggca ggttatcaat taagcgaaaa aattaaagaa tggctggcag aatttcctga
    28081 gcacccatca caagaacaat tgcttcctta ttattttgat ttgcttcatt ttttgaaggt
    28141 cagtgaattt tatgatgatc actacgaaac aacagtcgaa aaaacgtatc gcgatctgat
    28201 agtaaaagag ttttgtattg atccaagctt gtttcttgag cagtcgttgg ataaaggaaa
    28261 aagcagtatt cttttttctg ctagtttttc accactctct tattatcagg aaacactggg
    28321 gggacaaaaa agtctggctt acaaactgcc gagtcctttc ccagaaggaa accagcaagt
    28381 tttcatcgca aattacctag agacgactta tcgaaaaagg gaacacagtc tggcaaaatt
    28441 agtagagacg attcatgctt ttgctgatgg aaaggtcggg aattatttcg ttttctttcc
    28501 ttcttatcaa tatttagatc ttgcggtgga agcattcaga cagcaatatc ccgacagcaa
    28561 tgtgctaatc caagaaacgg acatgaatga ggaagaacgc gaacgatttt taacaaaatt
    28621 tcaaacaaat cccgctgaaa cattgattgg attttgcgta cttggcggga ttttttcaga
    28681 aggaatcgat ttgagagggg accgattgat cggcagtatg atcgtaggtg taggtttgcc
    28741 gcagatgaat catgagcagg aactgatcaa atcatattat gatgagaagg aacaccttgg
    28801 atttgcttac gcttatcagc ttcctggaat gaacaaagtc ttgcaagctg gtggtcgagt
    28861 gattcgtggc atggcggatc aaggaatcgt tgtattggca gatcaacgat tttctagttt
    28921 ttcttatcga cgtttattcc cacaacattg gcaaacagca agagaagtac gaagtgttgg
    28981 ggaattggaa gaaggaatac agcgtttttg gttatcaaaa gaagcagtaa atcaaaaaaa
    29041 cgaggaatga cagacgtcag cctcgttttt tgattaacga tagttagtga attgcaactc
    29101 gattggtaag tcaagtttac gtaataaagc aatcacagct tgtagatcat cacggttctt
    29161 acctgtcact ctaatctgat cttcttggat ttgtgctttt actttcagtt tttcattttt
    29221 gatagctgta gtgattttct tcgcgttttc acgatcgatt ccgttgacca aatcggcagt
    29281 ttgtcgagct ttgccaccta gagcgtgttc tgattcggaa aaatggatat ttttgattgg
    29341 cacatttctt ttgatcagct taccaaataa tacatctttg atctgttcga ttttgaaatc
    29401 atcatcgctc aatacaacta gattgctgtt ctctaatttg atttctactg tagaatcctt
    29461 gaagtcaaag cgattggaca attcttttgt tgtttgctga atcgcatttt ttacttcttc
    29521 tgtattgact tctgatacaa tgtcgaagct agcatctttt gccataggaa ccctcctgat
    29581 atacatataa atatttacca tacatctatc ttatcatagg attagggcag attcttacct
    29641 gtttgctaaa attcttctca tttgagttcg aagtatttta ttttaataga taaactgtta
    29701 ttcttacatt tggaatacca aagtagggga gaggtgcttg tgagtaaaat tatgactgcg
    29761 tatcataaaa atgaaagatt aaagaaaata gtggttattt tgataaccgg attgaccgct
    29821 gcggtcgctt taaattattt tttgattcca gccaatgtct tttctgcagg gatgaatggg
    29881 atcgctcaaa tcatttcctc gcttttatat gaatggtttc atatcaagat agacacagga
    29941 acatttattt ttcttttgaa tatccctgtg tttgtattag ggtttctcaa agtaggaaaa
    30001 cgagcaacga tccttagttt tgtgaatgtt gtatgtgtat cggtgatgac gatgctgctt
    30061 ccagtcggac agatcactga taatattttg atgaatgccc tagtaggcgg tgttttatta
    30121 ggtcttggcg taggattatc tttaaaaatg ggctttacaa ctggtgggat ggatatcatc
    30181 tctcttgtac tttcacaaac aactggaaaa acagtcggaa actacatgct gattctaaat
    30241 ggtatcattg tagtcatcgc aggcttcttc tttaactggg aaagtgcctt gtatacgatt
    30301 atttccattt atgcaatgac tcaagtaatt gatgccatcc acacgagtca tcaaaaagta
    30361 acagcaatga tcgttaccgt caagccagaa gaggtaacag aagaactagc aaaacggatg
    30421 gtacgtggaa tgacattact gccgtctgtc ggtggctatt caaaaaaaca agggaacatg
    30481 atcatgatgg tcatcacccg ctacgaaatg tatgatcttg atcagatcgt tcatgaaatc
    30541 gacgacgatg catttatcaa tattttacca actcattcag tatttggccg gtttgcaaat
    30601 gaaaacgaac aaaaaatgta tcgaacaact ggcgtattgc cagaaataaa aaatcgttca
    30661 caaaaaaaat gaggaatcag gtttatttta gaataaattt aatctattac atctaataag
    30721 aacattttta taaatttaaa cgagaatctt acttctaaat tctcatttac aagcagctaa
    30781 aaaaagagta tgataaataa aaatcgtgtt aaggagtgtc gtacgatgag cgaagcaaac
    30841 caagaacggt ctggacaaga aatagaacta atcaaagaac gtattcttgc tgccttggaa
    30901 atggtgattg atcctgaatt aggaatcgac atcgtcaatt taggcttgat ttatgatata
    30961 gaattcaatc ctgaaaatgg agaaacagtc attaagatga ctctaacaac tatgggctgt
    31021 ccattagcag atattttaac agaatctatc catgacgcat taaaagaagt gccagaagta
    31081 acgaaagcag aagtcaaact agtctggtat ccagcatgga cgacagataa gatgagccgt
    31141 tatgcacgga ttgcgttggg gatacgatag aaatcttggc tgactagcta tgagaagcaa
    31201 ataggaaaaa caaaaaagcc ttttatcctt tctagaagaa aagagataaa aggtttcttt
    31261 gtttataaaa ttttgtctgc tatattataa gttaattaaa taaatgtgtt ctattgataa
    31321 aggacattca aatccctttc cttttggaat ccttcttttt tacatcagtt tgattttcgt
    31381 atagtattct ttctcatctt cacgataaat cgcagcccca tttttttgtt gtgctttaag
    31441 actgccaaga ttttttttat ttacagacaa aagtgcctca tcaatgccga ttttttttgc
    31501 ctcttgtagt actaatgcta atccttgtgt ggcatatcct tttttacgat aattagggga
    31561 gatgccgtaa ccaatatggc cagcgccgtt tcttaatgtt tcgttcagtt tatggcgcaa
    31621 attaaaaatt cctacatact catgttcatc actttctaaa ataaagacgg ttgcgggaac
    31681 gtaactctca ggaagatcga ttcctaaaga cgttttttcg cattttttta cataatctaa
    31741 aaattcagca aatgtatagc cataagcatt gttgataaat ccactctcat ttggtccaaa
    31801 gctagtttgt agctgataag ctttctttaa atctgcttcc catagtttga tcagtttcat
    31861 cgtattcacc tctttaagta accttattca tttgttttct actatagcac tattgttgta
    31921 aatagccatg cctattttta tttattgtgt catatcgtat taagaatcat cgaacaatac
    31981 aaatatgaat tacaataaaa aaattatttg aagttcgttc attcgttact tatgatctct
    32041 agaagaaaga aaacgtgaat tacttcagcg tctagaattc cagaaggata gtaatcaaca
    32101 gaagaaataa tcaatatcca aatgaaagaa gatagctgaa tcgagaaaaa gaggaaaaaa
    32161 gaaatgaaga aaaaaataaa atagttacta atcccagagt cgttcgttta acattgaaat
    32221 cgatcatatg gataaaacag attttttcac caaccctcaa tagatgatgg agttgctcgg
    32281 atgaattatt tgtgtagatg gcataaggga gggcgtacag aaatgaaaaa gaacaaaaat
    32341 atgttcttga caaaaaacac atgtaatcct aaactaaaag atgtgatgcg gctatggcgg
    32401 aattggcaga cgcgctagct tcaggcgcta gtgagggtga cctcgtggaa gttcgagtct
    32461 tcttagccgc ataatttgtt tttttataaa gagaaggtac acctggagcg atgagaaaga
    32521 aaataacata ttatctaaaa gaatatagga aaaattttaa acaacggaac cagattcaaa
    32581 taaatcaaaa aaaatttgtg tgaaggtggg acagtttaaa acctaataga ttgattcaaa
    32641 tagtagattt atagattctg atacaagaaa tactggcaaa cgatagttaa ggaataaaga
    32701 ctaaacttaa actcaaaaag aggtcatgcg aaagaataaa tcgcatgacc tcttactata
    32761 ttttgaatgt gtttaaacgt tcaaataatg aatcttagta ccaaccgttt gcttcccaga
    32821 atgctttcgc cgcttcccaa gaaccataac gtgaagtaac atattgttct gctactcttt
    32881 cttggtttgc agcagagtag tcaccattta agta
//
LOCUS       DAXWLF010000012        32715 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXWLF010000012 DAXWLF010000000
VERSION     DAXWLF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.012"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(180..1079)
                     /locus_tag="ACGE4U_000421"
     CDS             complement(180..1079)
                     /locus_tag="ACGE4U_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8392611.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1089..1445)
                     /locus_tag="ACGE4U_000422"
     CDS             complement(1089..1445)
                     /locus_tag="ACGE4U_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8392612.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1801..2097
                     /locus_tag="ACGE4U_000423"
     CDS             1801..2097
                     /locus_tag="ACGE4U_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFF8392613.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2741..3694)
                     /locus_tag="ACGE4U_000424"
     CDS             complement(2741..3694)
                     /locus_tag="ACGE4U_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8392614.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3717..4472)
                     /locus_tag="ACGE4U_000425"
     CDS             complement(3717..4472)
                     /locus_tag="ACGE4U_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8392615.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4469..5428)
                     /locus_tag="ACGE4U_000426"
     CDS             complement(4469..5428)
                     /locus_tag="ACGE4U_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8392616.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5421..6371)
                     /locus_tag="ACGE4U_000427"
     CDS             complement(5421..6371)
                     /locus_tag="ACGE4U_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8392617.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6521..6973
                     /locus_tag="ACGE4U_000428"
     CDS             6521..6973
                     /locus_tag="ACGE4U_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFF8392618.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            complement(7061..9328)
                     /gene="gshAB"
                     /locus_tag="ACGE4U_000429"
     CDS             complement(7061..9328)
                     /gene="gshAB"
                     /locus_tag="ACGE4U_000429"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFF8392619.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWKEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9515..10513)
                     /gene="ftsY"
                     /locus_tag="ACGE4U_000430"
     CDS             complement(9515..10513)
                     /gene="ftsY"
                     /locus_tag="ACGE4U_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFF8392620.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10525..11334)
                     /locus_tag="ACGE4U_000431"
     CDS             complement(10525..11334)
                     /locus_tag="ACGE4U_000431"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8392621.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11343..14924)
                     /gene="smc"
                     /locus_tag="ACGE4U_000432"
     CDS             complement(11343..14924)
                     /gene="smc"
                     /locus_tag="ACGE4U_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFF8392622.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14944..15630)
                     /gene="rnc"
                     /locus_tag="ACGE4U_000433"
     CDS             complement(14944..15630)
                     /gene="rnc"
                     /locus_tag="ACGE4U_000433"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFF8392623.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15978..17759)
                     /locus_tag="ACGE4U_000434"
     CDS             complement(15978..17759)
                     /locus_tag="ACGE4U_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8392624.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17785..18699)
                     /locus_tag="ACGE4U_000435"
     CDS             complement(17785..18699)
                     /locus_tag="ACGE4U_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8392625.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18726..19688)
                     /gene="opp4B"
                     /locus_tag="ACGE4U_000436"
     CDS             complement(18726..19688)
                     /gene="opp4B"
                     /locus_tag="ACGE4U_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFF8392626.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19688..20632)
                     /locus_tag="ACGE4U_000437"
     CDS             complement(19688..20632)
                     /locus_tag="ACGE4U_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8392627.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20633..21637)
                     /locus_tag="ACGE4U_000438"
     CDS             complement(20633..21637)
                     /locus_tag="ACGE4U_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8392628.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21905..22147)
                     /gene="acpP"
                     /locus_tag="ACGE4U_000439"
     CDS             complement(21905..22147)
                     /gene="acpP"
                     /locus_tag="ACGE4U_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFF8392629.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22269..23270)
                     /gene="plsX"
                     /locus_tag="ACGE4U_000440"
     CDS             complement(22269..23270)
                     /gene="plsX"
                     /locus_tag="ACGE4U_000440"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFF8392630.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23319..25355)
                     /gene="recG"
                     /locus_tag="ACGE4U_000441"
     CDS             complement(23319..25355)
                     /gene="recG"
                     /locus_tag="ACGE4U_000441"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFF8392631.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALTGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25472..27154)
                     /locus_tag="ACGE4U_000442"
     CDS             complement(25472..27154)
                     /locus_tag="ACGE4U_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFF8392632.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27206..27568)
                     /locus_tag="ACGE4U_000443"
     CDS             complement(27206..27568)
                     /locus_tag="ACGE4U_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8392633.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27838..28026
                     /gene="rpmB"
                     /locus_tag="ACGE4U_000444"
     CDS             27838..28026
                     /gene="rpmB"
                     /locus_tag="ACGE4U_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFF8392634.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            28264..30933
                     /locus_tag="ACGE4U_000445"
     CDS             28264..30933
                     /locus_tag="ACGE4U_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFF8392635.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(31024..31512)
                     /locus_tag="ACGE4U_000446"
     CDS             complement(31024..31512)
                     /locus_tag="ACGE4U_000446"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8392636.1"
                     /translation="MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIY
                     DRNDLAMVLIGMPGIEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDI
                     KLEDFSDYEAITTIIKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVI
                     GI"
     gene            31756..32157
                     /locus_tag="ACGE4U_000447"
     CDS             31756..32157
                     /locus_tag="ACGE4U_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392637.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            32292..32630
                     /locus_tag="ACGE4U_000448"
     CDS             32292..32630
                     /locus_tag="ACGE4U_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFF8392638.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         9200 a         6895 c         5819 g        10801 t
ORIGIN      
        1 caaaaaaaga ataaacgctt gcatctagta ctctgcgagt tacaaatcat ccgatcaaat
       61 aaacattcgc tgattatttt ccacaaaaaa agcagcatat ccacgataaa tggatctact
      121 gctcttactt atccttatgt tttacaaggt attttctttt acacattact aatgctgtct
      181 caatacggaa cgattgtcat cgcctgttca aattcctctt gcaaatgttg tgtttgatag
      241 gcttgaaagg ccggatgatg ggcacgcaag aggacacgta atttcatggg ttcatagcta
      301 tctaacagct gttctttcaa gacaaaatct cgggtcagct gccattcata ataataacca
      361 aaaggtctgc agctgtaaat cgtgtgaccg atggatttat cttcaaagcg acgatgcgta
      421 tggccaaaaa ctacttgttt gatatttgaa taacggtcaa gtaattctcc gaatgattct
      481 gatcctaaaa atgcatttaa tttgttccag cgctcataag gagcagattg atacatgata
      541 aatgcccgtt taggaacgaa atgagtggat aaaatcacct caagtttttt tgcttccagc
      601 tggtctagca aattttttgc agctgcaagc atttctgcat tgacctcgag atctgttttc
      661 ccacgctgga tgaaccgatc ataccaatac aaacgcttca ttcgtacgat tgcctcgtaa
      721 tctttcaatt cggaaaactg atagtcgtac cagccattca tccctaaaag aacggtctgc
      781 tcatttaaag caatataccg ttcatttaaa aaatgtgggt ctggaaaatg gtccatcatc
      841 tgttcattgg gcacatctgc caactcgtga ttgccgaaat gaaatgtcgt agataatcct
      901 ttattgacga aaaattcgac aacagctagg caacggtcta ctttattagc cgtatctccg
      961 gctagatgaa gccgagtgat cttgtgcttt tgcaaaactt gccaaagctg catcagctct
     1021 gattcgccga atttcttgat gtccacatgt aggtcgctga tgatcgccaa ttttcccatt
     1081 ttatccctct agtcttttcg aacaacttgc aagttgcctt ttctcaagcc gcttggtcct
     1141 tttaattgta aactattata agttggttct aaagcaagtg tggatggatc atccatgtaa
     1201 agtattgcac tgtctttgat tttgattggt ccaactggtg tatcagccac tacagaataa
     1261 agacttagat ctgtttgtgt atcttttttg acaatgatca atcgaaatgc cgtgtccaat
     1321 tggcaactga cttggctttc tgcaaatgga ccgtcaccgt tttcgatatc taataaaagt
     1381 tgatcgtctg cttctaattt attcttcaat acttcttgtg cttcttttgt cacttctaat
     1441 ttcatctgta tcacctcatg tcatagaata atcactttat tcatcagatg caactaattt
     1501 gtctggttct gactgttctc aatttattgg aaagtaagtc attcgtttat tatgatttct
     1561 cttatcacaa aagagacaac tttccctttt cttcgttcag tttcttgaac ggaaaaagac
     1621 ccaaaaagca acctgttatt tgtttaacct tattcctttc ttaaataaca aaaaatacta
     1681 taaaaaataa aaaaccttta acaactattg gaaaaagaat tactttttgt tcacggatta
     1741 ttaacaactc ttcggtagag taaacttatc aaataaaaag acttctttgg aggaatcact
     1801 atgaaggaat atataaaaaa catctatttt atcgaagaaa cacaaaatat cgagggttct
     1861 tatattgaag taaaaacact ctttgtcaat gaagataaaa ccaaagcatt ggatatttat
     1921 aaaaaattag cttcaaaaaa aacgaatagt tttggattga tattaagcga gtataaaatc
     1981 aaagcagaag agagttattt ctatcaatta ttaaaacggt ggagtaagct tcccgccgat
     2041 ttttatcgaa aaatgcagat catcaactat caaccgcttg ctgagacaca tgcctaaata
     2101 gaggttgtga aatcagtgct ttgagctaat accgaaggtc agctgatgga acatcgttta
     2161 gaagagattg tgacaactac gatttgcttt aagtaataag aacgctggac agaaaatagc
     2221 ttctcatatt ttctagtcta gtggacttat tgccgagaag cagcagatgg aacatcgttt
     2281 agaagagatt gtgacaactg cgatttgctt tgagtaataa gaacgctgga cagaaaatag
     2341 cttctcatat tttctagtct agtggactta ttgccgagaa gcagcagatg gaacatcgtt
     2401 taaagaagag atcggtacaa aataaaaata tctaaattta agttttatct aagtttcgct
     2461 ttgtaccttt gattcaaatc aaaggaggcg atcttgatgc tgaaaaaatg gatcacacta
     2521 ttttttgcca ctccccattc tagaatcaac ccgtatgatg tagagaatga attcacgttg
     2581 aaaaaagatt tcccaaataa ataaaaacca tccgtaaaat catcaatacg attttacgga
     2641 tggtttttga taacttcttt gaggataggc tcttgctatt taagaatgta agaaacatta
     2701 ttgttacata gtatgagagt ttaatcctct gttatttatg ctatttaaat gcttgattca
     2761 catcttcgat catcagtttc attgattcta aaccgccccc gcttaaatac cagacatctg
     2821 gttcaaggga gatgatctgc tgatttttac cagcattcgt ttgtgctacc agttcattgg
     2881 cagaaatatc atctttgctg tcgtctccac caatagcttt tgttctgtct actacaaata
     2941 agatatccgg atttttttcc agcacatatt cataagaaac actttgaccg tgagtggatg
     3001 cttcgatttg atcatctgct tgttcaaagc caaataaatc atggatgaaa ccgaatctag
     3061 aaccagatcc gtaggctgat aattgccctt cattcacaag tgtgactagt gcttttttgt
     3121 cagtagcttc tgctttcttt ttcgtctgat cgattgcact agataattcc tctaattgag
     3181 ttttagcttc ttcttgctta ccaaagatct cccctaaagc agtgacattt tgctggaaag
     3241 attcccatgg atttttcgca tctaaagaaa ggaaaatggt cggagcaatt tgacttagat
     3301 ctttttgaaa atctctttgc cggccagaaa tgatgatcag gtctggctga agctgattga
     3361 ttttttctaa gtctggttct ttgatcccac ctgctgattc cactttttta taggatgaaa
     3421 gatactcagg aaggctgctt gtagctgctc caactacctt atctccaaca ccaagagcat
     3481 ccatcgtatc taaagaacca ttgtcgaata cgacgacttt ttcaggattc tttggcacct
     3541 cgatttgatc gccattactg tcttttatcg ttaatgccgt ttcagcttgc gtactgcttt
     3601 tctttgtatc tgcctgactg gtgttgtcac tatttccaca tgctgctaaa ccaaatgatg
     3661 ctagaatcaa gatacctact actaattttt tcatctcttc tctcctaatg ttagttttag
     3721 aaataaagac agaatctgcg accttcaatc tcacaaattc gtatgtccat ttcgtacaat
     3781 tcgtccagta catctttttt tatcatttct tgggtcgttc cttgcgtgaa taactgccca
     3841 tctttcattg caataatttc gtctgcataa cttgcagcaa agttgatatc atgaagaaca
     3901 ataacaattg ttttcccgat ttcttgtact aattttttta agatctgcat gatttgaacc
     3961 gcaaaattca tatctagatt gttcaaaggt tcatccagta aaatataatc cgtgtcttgc
     4021 gctagcacca ttgcaatata cgcacgttgg agttgaccgc ctgaaagagt gtcaatcgat
     4081 tgatccgcta tttcttctaa cccaaggtgt tccagtgatt cctgaacgat ttcctcgtct
     4141 tttgtcgtca attttccttt cgtataagga aatcgtccaa attctactaa ttctttcacg
     4201 gtgatttgga catttatttc attcgattgt cgtaggatcg ataatttttt ggctaattca
     4261 ttggatttcc aagcctttac ttcgttatgg tctaaataga tttcacctgt atcttttggt
     4321 atcaaacgac tgatcatcga caacaccgtt gactttccag caccatttgg accgataaaa
     4381 gcagtcaact tcttttcagg aatcgttaac tgtacatctt taaccactga ttttccatgg
     4441 tacagtttat tgaccttttg taaatcaatc ataggatact cttcctttcg cgaatcaatt
     4501 ttgtgacaaa ataaattcct ccgacaaatt caacgacgac actcactgtt gtattccagc
     4561 cgaaaatctg ctctactgcg agctgtcctc ctgtcagaaa cagcatagct accagaaagc
     4621 ttccgataaa tagctgacca tgacgataag tagaaaagac acgataactg atatttgcga
     4681 caataaaccc taaaaaagta actggaccca caagagccgt tgatgtccct gtcaaaagac
     4741 tgacacaaca taaaatcaaa aattcaaaac gatctgtgtc tatcccgagg tttactgctt
     4801 gttcatttcc gagatggaaa acgtctagca catgtcgata tcgaaataac aggtaccagc
     4861 agccgataat gatcaaagaa gcaggcaaaa gatactgact ctggatattg ccaaagcttg
     4921 caaataatct gccttgaagc aaatcatatt cattcggatc catgatgact tgtaaaaagg
     4981 tactgacact accaaaaagc gtacctaaaa tcatccctga catcagtaat aaaaaaagat
     5041 tcgctctcat ttttttcaaa agaaagaaaa ataacaatag actcagcccg accatcagcc
     5101 caacgttcaa taagaaaaaa actgtacctg tctgctgaag catcgtcact cctccgataa
     5161 agaagaaaag gaccgtttgg acaaggacat ataacgactc aagtcccaat actgaaggag
     5221 tcaaaaactg gttatgcgta attgtctgaa agctgattgt ggcactgctg acagctagcg
     5281 agacaaggat aaatgcaaaa attttcttac tcctcaatgc ccatgcaaaa tgccagtttc
     5341 catatgtatg gatcgtcaag tacattcctg taacaaataa aacaagaaca agcataccaa
     5401 taaaaataga tggctttttt ttaagcatat cgtcgccccc ttatcagaag ataaaggaag
     5461 atagccccac cgatgattcc aacgatcaca ctgactggta tttcataagg aaaaatcaac
     5521 acgcgagcga caagatcgca aaatactaag aacaaactac ctgctaaagc agttacgcca
     5581 attgttttgt gcacttgatc tccatatatt ctagagacaa gattcggaat gatcactccg
     5641 ataaaaggaa tgcttcctac catgatcaac atgactgcac tagccaatga tactaagcca
     5701 atgcctaaaa actgcatctt ctggtaatct attcccaaat ttactgcaaa cgattcgccc
     5761 agaccaatga tcgtaaatcg gtaagcaaat agccaaacca agagcaacaa tggaacaaca
     5821 gcatacaata actcataatt tcctcgtatc acagtcgcaa aatttccttg aagccatgaa
     5881 gtcatatttt gtaccaactg gaattgataa gcaaaaaaag tagcaactga accaatgata
     5941 ttccctaaca tgactccaat caacggaagc atcaattgat ttttctgggg gaacaatcgc
     6001 gtaagggata aaaaaagcaa tgttcctgca aaagcaaagc aaaatgctgt caaggatcgg
     6061 acaatcaagg atgcaccagg taggaaaatc atagccacta aaatacctaa acgcgcacta
     6121 tccatcgtgc ccgcagtcgt tggtgaaaca aatttatttt gggtcaaatg ttgcatgatc
     6181 aacccacaaa tactactggt cgctcccaca aggatcaaac aaatcgttcg tggcaaacga
     6241 gtcactagca agatttgtaa gctttgccca tctaactgga gcagttctga caattgtaca
     6301 ttcgatactc cgatgaacaa tgaaatacta cttaaaataa ctaataatgc tactagtcgt
     6361 ccgtagcgca tccaatccac ctttctaatt gagaatgatt ttcattctta ttttaagtga
     6421 atcgttgaac aaaagcaacc gctttttcat atgttttagg tattattggg aatatgctat
     6481 aatcgtaaga aagacaaaga gaagaaggag ggtatttttt atggcacaag atgatgtaaa
     6541 aagccatttg gataagggac tttatggtac acctttagtg aaccctgaag aacagcataa
     6601 atatttaggg acattcaggg aacgctgcta tcttagtatg actgttgctg aaatgatcca
     6661 accaaaaaac aaagaaaact ttttaaaaga actcaaaaaa catccagatg cttccatact
     6721 cttgaatggg gctctacccc tttctgtcca aaaccaatat atccagctag caacgaaaaa
     6781 caatagccga tttactgtag tcaacgatta cgtcactgca aatcctgagg aattcggatt
     6841 gctcttaaca gcaaaagaag cagtaaatga acctgtgatt gatatcgaaa aaaaataccc
     6901 agaagaaaac tcttcgccag ttgaaaaaga aaaaccaaaa aagaaaaaaa gcttctggga
     6961 tcgtttattt taaaacgaaa aaacacataa atttttacat gcaaaaaagc ttttgatcag
     7021 attgacatca aataggacaa tctgtcaaaa gctttttctt ttaaaatact tcaggatata
     7081 acaatttcaa tacattcatc gtcaatcttc tgccttttcc tgcaaaggga taaacatgca
     7141 tatgcatggc tggattgaaa tttgcttcga tgatcccata tgctttttta tcagttgtag
     7201 gatcgatttc tttatctggt ataatcaaat cgataccact aatcttagct cctagtgctt
     7261 ctaccgcaga aacggcgatt tttttgtaag cttcagaaaa ttcatcagtc atatcgatcg
     7321 aatcaccacc tgtactgata tttgagttct cccttagata aacaatttga ttggcttggg
     7381 gaactgattc gatcgtcaac ccttgttctt tcaacatcaa ttgttctaat tcacctaatt
     7441 ggatcaattc taaaggtgca cgatgattcg ttccacgcaa agggtcacta tttttttctt
     7501 caacaagttc tttcactgtt cggataccgt caccaataac attagcaggg actcgcaaca
     7561 tgatggcttg gacttctccg tcgatcacaa agaaacgata ttccgttccc ggcataaatt
     7621 cttctactaa aaccgaactg tcttcacgaa aagcaatggc taaacctgct tgataatctt
     7681 ccagactagc tccttccttg aaaatcgtga tacctaaccc gtaattggtt gatttaggtt
     7741 tgatcacaaa tgcctgctcg gcaaatctcg gataagcttt taccgcttct tccatggatg
     7801 aaaattctgc gcctcctggt acccggaacc cagcttcttt taagactttt ttcgttactg
     7861 ttttgttttc catgataagt gggacgatat agctgtcctt acttgtcatg tttgcatttt
     7921 tcacatattc tacatggtct tggaattgca gacgcaaaaa ttgatccgac tcatctaata
     7981 ctttgacttg gacacctttt tggatggcat cgaacataaa tatctgagta gaaagttcca
     8041 tttcgcgaaa accggccaac tgatagggac gttcatggct ttctttataa tattgatgtc
     8101 ccaatgccac agcaacttct tgctgactcg atagctgtgc ttcttgatag agtttcgctg
     8161 ctaacgtttg ttcaggatga tccatttgag tcaatgcttc ctctactaaa taaagagaat
     8221 ccaaccaatc caattcaacc agcatttgct tcatcccttc aagaatttct ctaccttctt
     8281 gatgaaattc tgtttgatct gatggttgtt ctagtgctac tttgttattt cgagtcgtac
     8341 cttctgctac ccattggtca cagtcttctt tttcttcttt ccataacatg tacatcagga
     8401 ataattgtaa gaaccgaact tgctctggat tgatacccaa tcttgcataa ggattcaaat
     8461 cgatattccg caattcaata taacgaacac ctgttgttgc taaatctgcg actttctttc
     8521 cgcctcggaa acgaagcggt gtataaaatt ctttctcctc cgaaagtttc ccttcttcga
     8581 tcattcgctc gatatctgct aaatactgtt tcatagaagc ataggaaact ttcacatttg
     8641 gatgattcgt atagcccagc gcactgttac gaatactgcg aacaggttct tgcggatgtg
     8701 attcatctaa aaagtagttc ttctcactca ttggtgaagc accgaataaa taagtgatca
     8761 tccagcgata gcgcataaag tttctcgccg ttttcaaata aagttctgtt ttgaattcgg
     8821 aaaaatcttg aaattcttcc tgatggctga acaatgttct tagcaactca tcgccaaatt
     8881 caaaattgaa atggataccg cttaccatct gttttcgctt cccgtattct tttgctaaat
     8941 aacgtcgata caagacatct tcgaaatttt ttaattttgc aataatgatc gcttcgtctt
     9001 tttccggtaa ggcaggtggc atacttaatg gccagatcat ctcttctttc ggtatagaac
     9061 gagcagtcac atcataaaca gcagcgagat actgaaataa ttcgggaata gaatcggtaa
     9121 caggagtgat catctctgtt tgtgtttcgc taaaatctgt ttggatatag gggtgatagg
     9181 atcgatcgcc aaagattgct ggatgatcgg tttttgctag atttcctgca agatcaaccc
     9241 gctgtccttc ccgttctata ccaaatcgag cttggtcgat aaaagggcgc gcattgacat
     9301 gcaataataa ttgcttaaaa ttcatcatta agtgacctcc aattcatatg aatctaatta
     9361 taatcaaaaa gtctaagaaa aagaacgaaa ttgtttgaga accgcttaca aataagtata
     9421 aaaatgaagg catcaaaaac acagctatgt taaaaacaat cattcaaaca aggagctgca
     9481 acttttgtca cagctcctgc tttttagaca tgggttattc ttcttttagt aatcctttga
     9541 acaatccaac gacaaaatca tttggatcaa acggctccaa gtcatcgatt ccttcaccaa
     9601 gaccgaccaa tttcactggt aaatgcaatt cattacggat agcaagtacg ataccgccct
     9661 ttgctgtacc atctaatttt gtcaaaacaa gaccggtcac atctgttgtt tctttgaatt
     9721 gttttgcttg aaccatcgcg ttttgaccag ttgttgcatc tagtacaaga agtgtttctt
     9781 ggggtgcttc tggtaattcc cgctggatta ctcgttttat tttttctaat tcgttcatca
     9841 agttcacttt attttgcagt cggcctgcag tatcaactag taaaacatct gcctgctcta
     9901 tctttgctct ttccaaagca tcgaatacga cagctgctgg atcgccgcct gcattgccac
     9961 gaacaacttc aacaccagcg cgttcgcccc agacaactaa ctggtcgatt gctccagcgc
    10021 ggaaggtatc tgctgctgcc atcaatactt ttttaccttc gtttttgaac tgatgggcaa
    10081 gtttaccaat acttgttgtt ttccctacac cattaacacc gacaaacaaa ataactgtta
    10141 gtccgtcttc ttggatattt agttcattta cttcattgat tccttccgct tcatagagat
    10201 ccacaagttt ctcgatgatc gcattttgga cttctgccga ttttttcgca tttctgagtt
    10261 tcacttcttg acgtaatgct tcggtgattc gtatcgctgt ttcaaaacct acatctgcac
    10321 cgataagtgt ttcttctaat tcttcaaaaa agtcttcatc gactgttcga aaattggcaa
    10381 acaactcatt catccgttca ccaaatgttt tacgcgtctt ttctaaaccc ttgtcgtact
    10441 tctcctgtac ttcaacttct tctgttttct cttcgccagt aaaagcacgc ttgattttat
    10501 caaaaaatcc catttgactc ccccctactc taatacaaat ttttggatca ctttggctac
    10561 gccattttct tcattgctgg ctgtcacgaa atctgctgct tctttgacga aaggcacagc
    10621 attctccatc gctacgccta gtccagcgta ttggatcatc ggtaaatcat tttcttcatc
    10681 accgattgcc atgatttctt cttgtttcaa accaagatct tttgccaata aagaaatacc
    10741 ataagctttt gtaacacctt ttggcataaa ttccaaaagc atacttctcg ttttgatgat
    10801 ctcatacatt tcatggaatt ctgctggtat ttctttgatt ttaggatcta attcttcttg
    10861 gtcataagcg attactgcct tattaagcac aaattcatct gtaatatcgc ttatatccgc
    10921 tggttggaat tgcagcagct tattcaacac attgtacaag gattgcttcc ttggggcact
    10981 aggcagctgt aaaaccacat tgtcagacaa aacatctagt ggcagatcca attgctgagc
    11041 cagtttgtat aagcgttgga tatctgatac ttccatcact ttcttttcca tgatttcacc
    11101 agtatcattt ttttgtacca aaccgccgtt gaaagtgatg ctgtagtcac cttcatctac
    11161 cagtcctaat tcttgcagat aatgcaccat cgcagctaag gggcgtcctg tacatagaac
    11221 gatcttgacc ccttgctgtt ttgcttgtgc taatgcttgt ttattctcat ctgaaatcag
    11281 cttttgttca tttaataatg taccgtccaa atcgattgct actaatttga tcattttcgt
    11341 tcctacactt tcgtttcgtt gattttcgcc tcttcattga tatcttccat ccgtacagaa
    11401 acgatttttg atacaccgga ttcctgcatc gtcactccgt acagtacatc tgctgcgacc
    11461 atcgttcctt tgcggtgtgt gactacgata aattgtgtgt catcttggaa atcgctcaag
    11521 taacgaccaa aacgtttcac gttggcttca tctaacgctg cttctacctc atctaagata
    11581 caaaacggaa caggacagac acggataata gaaaagagca acgcgatggc tgtaagtgct
    11641 cgttctcctc cagaaagcaa acttaaactt tgcagttttt tgccaggagg ttgtacttca
    11701 atctcaatac ctgttttcag caaatcagtt ggttctgtca gtactaattc agctttaccg
    11761 ccgccaaaca tatttggaaa gacaattttg aattcttgac ggatagcttc aaatacttct
    11821 ttgaatcttg tccgcacttc atcatccatc tcatccatcg tttcaaacaa ttggtttttc
    11881 gctgctaaca gatcatctcg ctgtgttgct aagaatgtat gtctttcact gacttgttcg
    11941 tattgttcga ttgaatttaa gtttactggt cccagttttt caatctgttc ttttaaggat
    12001 gaaacctttg ttctactgtc ttcgatatcg gttgtttctt gatagtcttg ggaagctttc
    12061 tcaaatgtca gctgatattc tgactgcaga taggtcaaat gattatctaa aatcatttca
    12121 gctcggtctt tttggacttc taatttggat tgttccgaca gtcgtgcttt ttgctcacga
    12181 ttacgctctg ccaagactgc ttcaagctta tcgatctcct tttgccttct atctcgctgc
    12241 tctttggctt ttgccaactc ggctttcaat gtttctcgct gagcagataa ttcgttaatt
    12301 tgtttttcca aactttcttc tgtgacttca tgatcagaaa agtctgctgt caatgtcgcc
    12361 aactgctttt ctatcgcttc ttgacgttcg gttgcctctg ctttttggac tcgggcaccg
    12421 cggagctgga tctgcagatg attaaactgc tctttcaaga cagcaagatc tgcttgttct
    12481 tgtgcttttt gactttgtac ttgtgctctt ctggcttcca tctggtcgct ttcttgactt
    12541 aacgatttga tttcttcgtc gattttttgt cgttgcgatt caagatctgt ttgtttgtct
    12601 ttcaattcgt tcgtttgctt ttcgtaatct tcgataaact gctgcagttc acgtgtctca
    12661 aaagttgaga tttgcttttc tttttcaaaa cgttcaagtt catttatgat gttttgcagc
    12721 tgatttgtcg cttcttgttc ttcaaaacgt aattgttcgc ctcgtgtacg caaaacttct
    12781 tgttcttcgg ctaaacgagc tgtttcttgt tgcagctcct gaactttttt ttcttgtgct
    12841 tggagttgtt tatctgcttc ttcaaattca ctagtcaact gtttcaattc ttgattttgt
    12901 acaaacaaac tgccttggtt tcctcgttta ttcgctccac ctgtcatgga tcctccggca
    12961 ttcatcacat caccttctaa ggaaaccact cgatattgat agcggatcgt ttgcgcgatt
    13021 gcattggcac tagttaagtc tttggctaaa aggatcgttc ctagcaggtt ttgaaccaca
    13081 gtttggattt gatcgggata actgacttgt tcactggcaa tccctataaa cccttctaca
    13141 gctgctgctt gtgtcaatat atgagccggt aattgtcttg gtttgattgt cgtcagtgga
    13201 aggaatgttg ctcttcctcc tctttgctgt ttcaaatacg tgatggcttg ccgagcatct
    13261 ttttcatttt caacgatgac atgctgggct gccccaccta aagctgtttc gatagctaac
    13321 gtgaaatctg caggaacatc gatcagttca gccactgctc cgacaatacc cgaaagctgc
    13381 tgtttatgct gcaagaccaa tcgaacccct tggtaaaaac caaaatagtt ttcttggatt
    13441 tcttgaaggc ttttcttacg agcacgtagc tgctggactt gattcattaa ttgatacatt
    13501 ttaggctgtt cttttgctaa tgcttcttgg aactttttcc cgttttgttg gatcatttct
    13561 aacttctctt ttgtttcagt taaagatgat tttagctgcg cctgcttttg tgtcaattct
    13621 tctttttgta agatcaatcg gtctactgat gcctctactt ccgattgctt ggctaacgtc
    13681 tgcttacttt ttgctgtttc ttgtacatat tgacgttcta gatacttcag ttcgtttcct
    13741 acagctgctt gctcttgcat caaatcaaca tattgatctc ttagttctgc cagtaattct
    13801 ttcgttgatt tttggtattt ttctaattct tgctgtgttt ttatgagact tgcttcagct
    13861 ttttggattt ctgtttcttt ttctgctact tctttcaaca agctttcttg aagcttttca
    13921 aaatgtttaa cctttttttg tgcttctgct aatgaagttt gatattcttg cgagcttttt
    13981 tgtgtatgct ttgtccgctc ctgcaaaaca tctttttgtc cttctgtctg cttcagtttt
    14041 tccgaaagat ctaataatac ttgctgattt ttttcgatca gacgatcggc ttgcgcattt
    14101 tctttacgct gcttcgccaa aatggattct tgttcttgga tcgactcaga tagtttgcct
    14161 aattctagat tgaatttcgc aagctgtgct tgtttgttgt cccaatcttt ttttgctgtc
    14221 ttgatttcgg caaccatcaa agagacatcg gtttgagtca gtgtttcttt tagccgtaaa
    14281 aactcctttg ctgcctcact ctgggcggcc aaaggagtta actgttcttc caattcgtgg
    14341 atgatgtctt ggacgcggct gaggttgtct tctgtttcaa aaagcttctg ttctgctttc
    14401 tttttccgct gcttgtattt caagacacca gctgcttctt caaagatccc acgacggtct
    14461 tccggtttac tactgaaaat cgcttctact tttccttgag aaatgattga gaacgattct
    14521 tttcctaatc cagagtctaa gaacaattct tggatatctt ttaatctgca gctttgttta
    14581 ttgatgaaaa actcgctttc cccagtccgt cgataacggc gggttacact gatctcattg
    14641 tactccaatg gcagataatg atcactattg tctaaaatca ctgtgacttc tgcaatattc
    14701 agctgcttac gggtatcgga accggcaaag atgatatccg gcatttttcc gcctcgcaga
    14761 cttttagcag attgttctcc taatacccag cggatagctt ctgtaatatt gctttttcca
    14821 ctgccatttg gtccaaccac tgctgtgacg ctattttcaa agtcaatgac tgtcttgtct
    14881 gcaaatgatt taaaaccggc tatttcaatt cgtttcaaat acaaagcttt ccctcactta
    14941 cttttacagt gctgccaatg cattttccgc cgcagcttgt tctgctaatt ttttcgattt
    15001 tccttgtcca gtgccaatca attgatcatc tacataaact tcgatccaaa atactcgttc
    15061 atgagctgga ccttcttcat tgatcaatcg gtattcgatt gatacatcac ctgatttttg
    15121 caagacttct tgtagttttg ttttatgatc catctcatgt gaaaaagcac ctgctttgac
    15181 tttaggaaaa atcacttttt cgatgaatgc atgggtcgtg tcgaatccct ggtcaagata
    15241 taacgcgcct aaaaaagctt cgaataggtc gcaaagcaat gctggtcgcg tacgtcctcc
    15301 tgaattctct tctcccttgc ccaacaaaac atattgatca aagtgacatt ctttggcaaa
    15361 tttcgacaag ctgtcttcgc gaacgatcgc agctcgtgtt tttgtcaatt ttccttctgg
    15421 caaatgagga tataaacgga ataaatactg agaaaccatc agttctaaaa cggcatctcc
    15481 taaaaattca agtcgctcat tatccgataa ttgcagattg cgatgctcat tcacatagga
    15541 tgaatgggta aaagcttgtt caagcaaatt tagatcgtga aaaacgatgt tatagttttc
    15601 ttttagttct tttgtcagct ggttgtccat tttccacatt ccttttatgg atattttttc
    15661 gatacttcct tattatactg gttttttgtc aaatttgaaa tggttcggtc aaaaggtaag
    15721 taaagcttta ataatcctag tgattgacga gaagttgtga aaaattcgtt tagtttcgag
    15781 tatgaagaaa aaattttgtg aaaatggctt tccatatttt tgcataaatt tgacttaata
    15841 ccgagaaact ggactttgaa caccgtttat tggagaagtc atgaaatcag cgccttgacc
    15901 tgaatattag agaaataaac agaaaaatat caaaaaaccg aaaaaggaca tcgccctttt
    15961 tcggtcattt ttagtgatta tgaacgtgaa tctgctgtaa cagaaacatt tgcccatggg
    16021 tttggtacat cataagacca atcccagtct ttgacacggt cgttgactgg caatacttca
    16081 ttacggaaca acgttggaat cgcaaatgct tcttcaaatg catattgttg ccatttttga
    16141 tatgcttctt tttggtactc ttcatcaaat gctttgcttg aactgatatc gttgatcaat
    16201 tttgtatttt cttctgattc ataacgagtg tagttgaacg cagcatttgg tccccatagt
    16261 ccacttggat ttggatcagt tcctgtattc caagcagctt ggtaaacatc aatttcagga
    16321 tcatcatttt ctaatttatc atagaaagaa ttgaattcga tcagacgacc tgttgtgtat
    16381 tggacatcca aaccgatttc tttccactgt tggatatagt aatctgccaa tggttgcgct
    16441 gtttccccac cggacatcga agcaaagttg attgttagtt tatttccatc tttgtcttct
    16501 cttaaaccat cgtcatccac atctttgtag ccagcatcat ctaatagttt tttcgctttg
    16561 tctagatctt gtgtgaatcc tttcatctca tcatcatggt atgactggaa tactggagga
    16621 atcaatgaag tcgcattgct tctcaaacct gcatagaaac gctcacctac tgcattatta
    16681 tctaatgcat agcccattgc ttgacgtaat gatttatcag ccatttttga atctggattg
    16741 tattccacac tctttgtatc tgcattccat ttacctaact tgaatcctag ataagtataa
    16801 gaagtttgtt cacggcctaa gtttgtatag ccagcaattt ctttataggt atcgtaagta
    16861 tctgttggca tttggaaaat catatcatat aatttagagt tcaatgcttc agttgcactt
    16921 gccatcggta cagatttcat cgtgatcttt ttcaatttag gcgtaccatt ccagtagtgc
    16981 tcgtttggca agtattctac tgattctcct gcaacgatat tactcatgta ataagggcca
    17041 aaagtcactg gatttttacg tacttgatcg cttgattcca tatctttgat tgctacacct
    17101 tctaacgtat gttttggtgc tgcatatgac caaataccac cacccgattg aagcatgctt
    17161 gggtttactt ctttatattc gatttcaact gttttgtcgt ctattttttt gattccggag
    17221 attgtgtcgg cacttccatc atgatattct tccataccta cgatgttacg gaaagtgtcg
    17281 tcataacgga taccagtgta atctttattc ccaatgattt cgtaagggaa aatcacatcg
    17341 tccgccgtta catcttcacc atctgaccat ttgacgttgt cttttacttt gattgtcgct
    17401 tttttcgtat cttcatctag atcaagcgtt gcagctccgt cgtctgtgat tctgaaatcg
    17461 tcatcgtttg caaatagcgg ttcatgagaa ggagccataa aagctgcatc atatgaatct
    17521 tggtaaaact cccactggaa caatccttta aactgactgt ccgttgcgac cgcagcttct
    17581 aattcgccgt cttcgattgc atcatcatta ttttttgttg caattggaaa agttaattcc
    17641 tctttgttat tgccgttgcc tccagagttg tttccagagg tatctgtacc tccgcccccg
    17701 catgctgcaa gaacgacagc agacatcagc gctgcaaatc caaaaatcgt tttcttcatt
    17761 cctatttccc ccttcgcttc tatactagcc taaccgttgg cgcgcatctg cagaacgctt
    17821 gaatgcttgt ccaacataat tgatactcaa catcaaaact aaaataagga ttgatgcggg
    17881 taaccagatc cattgccgat tcactaagac ttctccgtta cttgcataac cgatcaatgt
    17941 acctaaacta ggtacatttg ccggcaagcc gaaacctaag aaagtcaaag ttgtctcgat
    18001 accgatgttt gccgcaaagt tcatcgtcaa attagtaatg attaatgaac ttagattcgg
    18061 catgatttcg cggaacatga ttttaaaatc attggttccc attgtttttg aggcactgac
    18121 ataatctctt cttgcttcag ataatgtctt gcttcggaat aatcgggctt tagctaccca
    18181 ataaaatgca ctcatgatga atataaagga ccagacattg tatctggaaa taatcgatac
    18241 gaagacgata atgatcagca tgatcggtag aatcatgata aaatcaacga tccgcatcaa
    18301 gatgttgtca atgatccctc cgtagtagcc agagatgatc cctaatccaa ccccgatgat
    18361 cgaagtaagg atcgtgatcg caaatccgat cgtaacagag tttctagcac ctatgatcaa
    18421 ttgtcctaat acatcacgtc cgccttcatc tgctcccaga atgaactttt gtccttgtgt
    18481 cgaaactgtt cctggtgcag cgtatttgtc gaagatacta acatacatca ctttgtcttg
    18541 atcggtcagc atcgcaccga taaagatcac taataataaa accactagca aacctaatga
    18601 aaaaatagca attttatctt ttttaaattc tcgagcaatc attcgtatcc ccataggagg
    18661 aatactttct tgaactgctg tattgttttt ttcactcatc gatccattct cctttctgat
    18721 tggttttatt gaatccgaat acgcgggtca acgatactca tgatgatatc tgaaagtaat
    18781 gtccccacta gcgtcgcgat tcctaaaatc aatactaagg atgtgatcac ggtatagtcc
    18841 cgttggccga tggaatcaat aaatagtttt ccgactcccg gataaccaaa aatcttttcg
    18901 ataactacag aaccagcaat caacgaagtg atttcatacc ccatttgaga agccatcggc
    18961 aacgctgcat ttcggaaaat atgtcgactg aatactttat tcgtcggtac ccctttggaa
    19021 cgggctgttc tgacaaaatc gagcgatttc gaatcgatta cttcacttcg cagatattgg
    19081 attgtgacgg cagtcgccaa aaatgcttgg gtaattgatg gtaacagaat atatttcaaa
    19141 cggtctatcc attgagtgat aaatccacct tgcaaaccta gtgtttgtga tccacttgtt
    19201 gggaaccagc ctaaacgata accaaaaaca aatagcatga tcaagccgaa gataaataaa
    19261 ggaacagcaa aactaaagaa gttgtagatt acgacaatct tatccaagat tgaattttga
    19321 taccggccag caagcatccc cagaggcaca gcaatcaaat aagtaaggac aactgtcaag
    19381 atcgatagat ataaagtgtt gatgatccgt tcgccgatca agtcagcaac agagcgtttg
    19441 tataagaaac tttgaccaaa atcgccttgc aaagcatttc cgatccaacg gaagtactgg
    19501 acataccatg ggtcattcaa gccagctgct tgtcgcatct gttcgatcac tgccggatcc
    19561 tgatttggat tgatcaaacc ggtaaatgga tcccccggca tcgcttgggc taataagaag
    19621 atcagaacac ttaaaataat aacttgcggg atcatcaata aaacacgtcg cagaatcgtt
    19681 ttccacatta gcttgcacct ccatctttca acgccacttt gtgtgtagtt gtgatcgtac
    19741 gcaaatcgta gactcgacca gtcgcatcat agtattcttt ttggttttgg atatactctt
    19801 gttctactct tcgtcgattt tctttgtggc tttccctatt ttctacatcg atttgcggaa
    19861 tagctgaaag cagacgtttc gtatagatat gtcgcgggtc attataaatg tcttctcttg
    19921 taccgatttc tacaaatcga cctttgtaca taattgcgat attgtcgcac atgtgtttta
    19981 ctacacctaa atcatgtgaa atgaataaat agctgaggcc gtattcttgc tggattcgtt
    20041 tcatgaaatt caatacttgc gcttgaacag atagatccag agcagataca ggttcatcgg
    20101 ctacgatcag ttttggattg gtagcaacag cacgggctac tcctagacgt tgacgttgtc
    20161 cgcctgaaaa ttcatgagga tatttatata gcgcatcttc cggcatccca acgatatcta
    20221 gcagtccttt tacctttttc ttttcttctt gatcgcttaa ccgttcaaaa tttcggatgg
    20281 gttcagcaat gatatccaac actcgctttt taggattcaa actggacatt gaatcttgga
    20341 agatcatctg cacatccttg ttataaccca tttttttgcg gttgcttcgc ttcgttacat
    20401 cttttccttc atacatgatt tgtccggctg tcaccttttc taatcccaca acggcttttc
    20461 ccgtagtaga ttttccagag ccagattctc ccactaaacc atatgtcttt cctttttcga
    20521 taataaaatc cacaccatct acagcatata catgatccgt tactcgattg aagaatccac
    20581 tgcgaatagg gtaatgaact ttcaggtctt tgattgtgat taattctgtc atctacacct
    20641 ctcctttttc gtctctaaag tggaaatgct tatagcagga gcaacgtacc aagtggttcg
    20701 gtgcgacttc atgaaggata ggctcttctt catgatcgct tgctggtatc catggaatac
    20761 gtggtgcaaa ccgacaacct ttacgaggca tgtttttcag agaaggaacg acaccttcga
    20821 tcacatggag ttctgcatca tgatttcctt cttgtggtat agagttcagt aaagagcgtg
    20881 tataaggatg cttaggatga tcaaacagtt cttttacacc tgctacttca acgaactgtc
    20941 cgccatacat cactgccact cgatcagcca tttctgctac gactcctaag tcatgagtga
    21001 tcaagatgat ccctgttttt gtttcttcct gaaggtcttt cagtaaatca agaatctgtg
    21061 cttggatggt tacatctagg gctgtcgtcg gttcatctgc aatgacgatt ggaggtttgc
    21121 aggcgattgc gattgcgatg atgacacgtt gtctcatacc gcctgatagt tcatggggat
    21181 attggcgtcc aactctcgca ggattaggaa tccctacttg ttctagcaat tctagtgcac
    21241 gagcttgacg ctgttctgca ttcatctttg tgtgatagga aagtccttct ttgatctggt
    21301 cttcgattct catgagagga tttaatgcag acaacggatc ttggaaaatc attccgatat
    21361 catttccgcg gattttatta tagagcgttt cgttcaggtc gattaaattc agatcgttat
    21421 ataaaatctc acctgtaatt tttgtgttat taggatcaag taatcccata atcgtagtag
    21481 ccaaggtact tttcccgcac ccagattccc caacgattgc taaaatctcg tttttgtcta
    21541 aagtaaatga aacgtcatct actgcatcgt aatagtcatc tttgagtcgg aatcctgtat
    21601 gtaggttctg aacttctaat aattggttat cggacaatcg aatacccctc tctgcttcct
    21661 aaatggaagt ttttcttaat aaaaaaggac attacacaga ataaagcgtt ttataacaaa
    21721 ggttattctt atctttgtgt aatgaattat taataattat atagaaaatc gctttacttt
    21781 gcaacataat ttctgtttat cgccagaatt atcagaaaaa tgacttttac gtgtcaaaaa
    21841 gattaaaaaa agaaggataa aaagctgtat gcagcttttt atccttcttt gcttttattt
    21901 gaaattattt aagatggctg ctgatataat ctactgcgcc accgactgtt tcaatttgtt
    21961 cagcgtcttc atctgaaatt tccgtaccga attcatcttc aagctctaaa acgaattcca
    22021 taatactaat tgaatcagct tttaaatcat ctttgatgtt caattcatcc gtcactttat
    22081 cggtatctat ctcaaaatga tttgaaataa ttttcgctac tttgttaaat acttcttcgc
    22141 gagtcaactt tttcacctcc aagaatcata cacttgtcaa ttacctacac catggctaat
    22201 tttactggca tttttcagga atgtccacct ttttttcaat ggaacggatt gcttcagata
    22261 tccaaatact attcttcgct tttttcaaat tgttcgacta attgtccaac tacatctgtc
    22321 tcaagcattg tgtggatttg gcggatcgta tagcgaacag catcggggcc tgttgctcca
    22381 tgagttttaa tgactggtgc tttcaagcca aacaaaacgg caccgccatg tttggaataa
    22441 tccatttctg ctttcaaaga acgtaagccg tcttttaaaa gcatagcgcc catttttcct
    22501 ttgatccctt catttagaat agcagatttt aatagattca tcatattcat tgctgttcct
    22561 tcaatagatt tcaaaacagc gttgcctgta aatccatctg tgaccactac atcagccaca
    22621 ccattgagta gctcacgagc ttctacgttt ccaataaaat tcaagcttgt ttcattctga
    22681 aggagttcat aggctttttt cgtcacttca ctaccttttg ttgcttctgt tccgttgttt
    22741 aagagtgcga cacgaggacg tccgatacct cggactttac gtgcataaaa agaacctaaa
    22801 ataccgtatt gcaacaaatg ttctggtttg ttttctgcgt tagcgccaag atccagcata
    22861 tcgaaaccgg caccttcttt ccctactact ggcaaggtag acatcaagcc agggcgttcg
    22921 atatttttga tacgtcctac gatgaacaac cctgctgcta gtagcgcgcc tgtattccct
    22981 gctgaaaata cagcatctgc ttcaccgttt ttcactgctt gtgcagcaag gaccattgat
    23041 gcagtctttt ttcgtcgaat tgcttttact ggctcatcat cactattgat tttttcgtct
    23101 gtatggataa tcgtgatgtt tttttcattt gttaagtatt tcttgatttc tgcttctttt
    23161 ccgtaaagta agaattctac ttctggaaaa tcttgctgcg ctaaagccac gccttctaca
    23221 attgcttgag gtgcgtgatc tccgcccatt gcatctacgg caattttcat tgtttccact
    23281 cctttttccg tcatcagtat aaagtatagc acagtctgct aatcgaaata acgattatcc
    23341 tgattttcta tttggaggct ttgcgctaaa ggctgatact caggaagcat ctgccaatcc
    23401 tttatctgcc aaagggctgc agcctcttct ctggctacct ccaaaacatt tccatccgtc
    23461 accaagtcag caatcgcaaa ttgaggcaga ccagattgac ggaagccaaa tacttcacca
    23521 ggaccacgta attccagatc tttttcgctt agtacaaaac cattggtggt ctctgtcatg
    23581 attttcatcc gttccacacc tagttcattt ttaggattag caactaagat acagtaggaa
    23641 gcatcacttc cgcgtccaac acgtccacga agttgatgca gctgtgccaa tccaaatcga
    23701 tccgcatcca tgatcagcat gacggtcgcg ttcggcacgt ttacccctac ttcaattaca
    23761 gtagtcgaaa ctaatatctg catctggttg tctttgaagg cttccatgat tacttctttt
    23821 tcctgattct tcattttacc atgtaacaag ccaacttcat attttggtgc aaatagttca
    23881 cgaagttttt catagatttc cactgcattt tttacatcta atgcttctga ttcttcaatc
    23941 agcggacaga tgacatacat ttgatgtcct cttgccagtt cttttttcgt ccaatccagt
    24001 acactgtcca attgaggtgt tcttacccag cgtgtttcga tcgggatacg tccagctgga
    24061 agttcatcaa tgatagatac atccatctca ccatatgctg taatagctaa tgttctagga
    24121 atcggagtcg ctgtcataaa taagacatct ggtcgccagc ctttttcacg aagtactttc
    24181 cgctggttca cgccaaaacg gtgctgctca tccgtaataa ctagacctaa attctgaaat
    24241 tcgactccat cttggatcaa tgcatgtgtg ccgatcacga tgttaatttc cccttgctct
    24301 agtttttcaa ggagctcacg acgttctttt gctttcgttg accccgtaag caatgcgatg
    24361 gttacttcat tgggatcaaa cagctgttgc aagctttcca tatgttgttg tgccaagatt
    24421 tctgtaggca ccatcaacgc accttggaaa cctgctgtaa cggtagcgta taacgcaatc
    24481 gcggcaacca ctgtttttcc gctacctaca tctccctgca aaagtcgctg catatgctgc
    24541 ggactgcgaa gatcccgaca gatttcattc gttacacgtt tttgtgcatt tgtcagttca
    24601 aaaggtagct tttgagtaaa ttctttcaat ttttggacat cataaaggat ctccaaacca
    24661 ttcgtttctg ctttttctgc ttttttcaat ccttgaagac gcatttgaaa taaaaagaac
    24721 tcttcaaaaa cgacacgtcg tttggcttga tggttttctt cgtggtcttt tggaaaatgc
    24781 atacttctta cagcagaagc tcggtcaagc agtcgatatt tctccacaag gttgtttggc
    24841 agattttctt caatcaatgt gccgtattct tgaaaccctt ttttgattag atcaactaat
    24901 gtcgtttgac gaattgattt gttgacgtga tatatcggcg aaaagtcatc cattgtttga
    24961 gaacctaaaa tcttcatccc ggtcaatgct ttacgctttg catcccattt accgtatacg
    25021 gcgatttcct ccgataaaat gactttttct tttagataag gttgattaaa aaaagaaacg
    25081 ttgaacacat catgttcttg catcatacga aattgcaatc ggcttttttt ataaccaaat
    25141 cgattcatca caggaggcga gactacgatt cctttcaaag taaccttttc ttgatcttgg
    25201 atctcgttta gatttctttc ctgaatatct tcgtagcgga aaggataata agtcagtaag
    25261 tcttcaatcg tttggatgcc caagctggca agactagctg ctcttttttc tccgactcct
    25321 gccagcatag tgattggatc atctaaagaa cgcataaatt tttcctccct cccttaacaa
    25381 aaaaaggact tggtcagcga tcatacctga tcactaaccg aaggcctttt atgaatgaat
    25441 actgtgttag aaagatattc tgacacagaa attattcagc agaaaataga taaggataga
    25501 caggctgatc tccttggtgg atctcgattt ccaaatcagg atgtttttct gttagtttct
    25561 cagcaaattc atttgcttgt tcaactgttc cttcatcacc tgccaagatc gttacgatct
    25621 ctgtgtcctc attgatcatt tgttctagtg tacttaacgc tgttgctgtg atatctgggt
    25681 tagagatcag gatttttcca tcgatcatgc ccaaaaagtc tccttctttg atctctacac
    25741 cgtcaattga ggtatctctg atcgcgtgag tgacttgacc gctcacaacg ctagacaaca
    25801 tttctgtcat cgtttctttg ttttcttcta aagaagcttg atcattgaat gaaagcatag
    25861 ctgtcaatcc ttgggaaatc gttcttgaag gaacaactgc tactgggata tctgctactt
    25921 ctgctgcttg atcagctgcc atgaagatgt tcttattgtt tggcaagaca atcactttat
    25981 ctgcgtttac ttcttgaatc gctttaacaa tgtcttccgt acttggattc attgtttgcc
    26041 caccactgat aacataatta gcgcctaaac tttcaaacag ctcttggacg cctttaccag
    26101 cagcgatcgc tacgatacca tacgggatac gtggtgtttc ttttttcgca aactcgttca
    26161 cttcttcatc gtgttccaaa atcgtctcat gctgcaagcg catgttgtcg actttgattt
    26221 tgactaatga accgaatttt tgtccataat tcaagacttc tcctgggttt tctgtatgca
    26281 cgtggacttt gatgatctcg tcatcattga ccacaagtaa agaatctcct ataccatcta
    26341 aatagttacg gaattcttcg taatcgaatg tactatcgac tgtaggtcct tctccgattt
    26401 ttaccatgat ttctgtacag taaccaaaat ggatatcttc tgtcgccatt tgaccttgga
    26461 cactgcgatg atgttctgca ttgaccatat catccatttc tgctggagaa ggttcatatg
    26521 tttcatccgc ttggaattct ccattcaatg cacttaaaaa gccctcataa acaaacagca
    26581 gtccttgccc tccactgtct acgacaccga cttcttttag gactggaagc aagtctggag
    26641 ttttagctaa agcacgcttt gcccctttta caacagctgt cattacttca acgacatcat
    26701 cagtactttt tgctttcttt tctccagctt tcgctgcttc tctagctact gtcaaaatcg
    26761 ttccttctac tggtttcatt actgctttat acgcagtttc aactccatga gtgaaggctg
    26821 cagcaagatc ggaagcattt aatgagacaa catctggaat ttgcttagaa aatccacgaa
    26881 acaattgaga caaaataacg ccggaatttc cgcgcgcacc catcagcaat ccttttgaaa
    26941 gcattgctgc taattctcct actttttctg aggttgaatc tactactgcc ttagctccac
    27001 tagtcataga tagattcata tttgttcccg tatcgccatc tggcacaggg aatacattta
    27061 atgaattgac gtattctgca tttacttgca gacgattcgc acctgcctgg accatttcct
    27121 ggaactggct tgcacttatt tctgttacac tcacctagat aatcctcctt cagatcgact
    27181 gctgagttac cagccagtct cctgcctagt ctggcagtac acgaacacct tgtacaaaga
    27241 cattaactga attggcagtt acgcctaata atgcctctag attgtatttc actttttctt
    27301 ggacgttgcg ggaaacttca gagattttcg taccatagct gataatcgta taaacatcta
    27361 cagcaatacc attatcttct tggcggacaa ctactcctct tgagtaattt tctttacgca
    27421 aaatctcagt aatattatct ttaatttgat ttttgctggc cattcctaca acaccgaaca
    27481 catctgtcgc ggcgccacca acaactgttg caattacatc attggtaatt tcaatggttc
    27541 cagctgacgt ttgaattttt acagccatga atgagcctcc ttaacgggta aaaaactccc
    27601 atttttcttc tatcatattt tatcatagcg caacttgaaa taaaaggcag ttacccaaag
    27661 tactcaaaaa ccttgcaaaa tttcaggtat aaccttgttt aatttacaat tattcttctc
    27721 aattgtcaat tgtatctatg aaaatgtact tgcaaatttc cattctatat gatatgttag
    27781 tctggtatga gctgaattat tctattgctc caaatcaagg caaaggagga aattaccatg
    27841 gcaaaagttt gttactttac aggtcgcaag acaaaaagtg gaaacaaccg ttcacacgct
    27901 atgaactcaa caaaacgtac tgttaaacct aacttacaaa aagttcgcgt tatggtcgac
    27961 ggaaaaccta aaaaagtttg ggtgtcaact cgtgctttga aatcaggaaa agttgaacgc
    28021 gtttaattga atgatacttg gaccgttcag atagtgaacg gttttttatt taataaaaat
    28081 ttttaagggt gggacttttg attcctaccc tttttttgcg tttatttttg ttatacgaac
    28141 aaattatgaa gttatttatt ctaaatttta tttttttgtg aaaattagac tctttttaag
    28201 cagagaaaaa caaggatatg ataaactgaa tacaacttca tagaaatttg gtggaaaata
    28261 aatatgaaaa aaaatttaaa gtcattcttt agagaaaacg gacgctacgc agcagctagt
    28321 ttcctgattc cgtttttgat tttagcactt gtttatctaa gtatcgggat ctatcctgga
    28381 agcagcagaa gtattttggc aagtgatgcc ttttcacagt tttctaattt tcatgccagc
    28441 ttccgaaatg ttcttcttgg aaagcaaagt attttttata catggaatgc ttctctagga
    28501 ttgaattatt tatctttaat ttcttactat ctaggcggga tcttcacacc actggtgttc
    28561 ttttttccaa accagatgat gccagatgca ctgtatgttc tgactttgct taaggtcggc
    28621 tgtgccggat tggctttttg gtttcttgca cgtacgttca agattcctcg ctgggggcat
    28681 gttgcactta gcgtcagtta cgcattgatc tcatttatca ctgcacattc ggagatcatc
    28741 atgtggctgg atgcatttgt ttatcttcca ttagtgattt tggggattca tcgtgttatg
    28801 gatcttaaac ggccgaaact tttatttatc agttatttac tcttgttttt atccagtttt
    28861 tatatgggtt ttatgattgg cgtattttct ttcttatatt ttatcgcccg tctattgaca
    28921 aactggtcag aatataaaaa aacgattgtt ccttatggta tcacttcttt actggcaggc
    28981 ggcgcttcaa tgatcattgt attgccagca gtgctggatc ttcggtcaaa tggcgaggca
    29041 ctgagtcaga tcactacgtt gaaaacagaa gcgacttctt atctggatct aatcatgaaa
    29101 aatatgattg gtgtttatga cacgacaaaa tacggatcga ttccgttcgt ttacgcaggt
    29161 ctgctaccat tagtcctctg tctgttttat tttgccagtc ggaaagtacc gttgaaaaat
    29221 aaatttttgt acggcagtct tttcgccata cttatcgcaa gtttctatct tgttccattg
    29281 aatttgttct ggcatggtat gcatgcaccg aatatgtttt tattccgtta tgcctatctg
    29341 ttctctttct tagtgctcct gcttgcagga aaaggatggg aaaagataga acaagacgat
    29401 cgtgggctgt tgttaggaat cattttggtc gtagctgctc tctttgcggt ggcatggggc
    29461 atcaaacctg aaggaagcta cacctatgtg acgatgactt cctttgtatt gacagtggtt
    29521 ttcttaggga tttatgcttt tgctgtggga ttttatcagc ataaaaaact ttcattcaat
    29581 catctgagta tattgttatt attgatcatg tctgctgaag cactggtcaa tacgaacagt
    29641 atgatccatg gcattcttga tgattggaat tatgcttccc gcagtttata tactgatcct
    29701 tatcctgact tgaaagctct tgtaaatgaa acaaaaaaag attccgactc gttttaccgc
    29761 ttggaaaatc tggatccagt atctgcaaac gattcgatca actatggtta cagtggtatc
    29821 agtttgtttt cttctataag aaatcgtaat tcttcctctt acctggataa attaggtttc
    29881 cgttcaagag ggacaaattt gaatatccgt tataacaata atactttgct aatggatggg
    29941 tttacaggaa tcaaatataa catttctaaa aacgaaagca cgttcagtaa atatggcttc
    30001 cgccaagctg gtcaaagtgg cgactataca ctgtatcaga atgtcaatgc attgccacta
    30061 gcatttactg caccgttgtc cgttaatgat gttgagcagc cagtgacaga taatttgacc
    30121 agtcagacaa acttgatcaa tacattgtct ggtttaaatg aacagtattt tacattctat
    30181 acacctactt tgaagaatca gttgaatacg acgatcacaa agacaacgac tggcgtcact
    30241 tactcagaaa cagcagataa ccagccaaaa gagctcactt gggaagtcca tgtgcctgca
    30301 aatacacaag cttacttaag tttgttccca accaattttg cacaattaga aagttctagt
    30361 gcgacgatca ctgtcaatgg tacatcgcga aaaacgcaaa tcaacatttc tggtcaatac
    30421 tatgatcttg gctattatcc tcaagagaca acagtgacat ttactgcaag tttttatgga
    30481 acgaaagaag taagtttcat ggagcctaaa gtcgttggtc tagatgttgt agcctatcaa
    30541 gccgcaatga atcagatcaa agaaaaaggt gtcgaaatgg aagccactgg cagaaaagct
    30601 tctgggactg taaataccgc tgaagattcc atgttggtga caactattcc ttatgacaaa
    30661 ggatggaaag ctaaaattga tgggaaacca gtcaaggtca gcaatttcaa agacgcattc
    30721 ttgatgataa aagttccagc aggtaaacat actgtcactt tcagttactt gcctgaagga
    30781 tttacgatag gtgcaatcct ttttgtccta tgtattgcat tgtttattct ttatgtctgg
    30841 taccttgacc gcaatcgccc cttttatggc acgattgatc cagcaataga agaaaaaccg
    30901 cgaaatacac acagaagaag acgaagaaaa taaagaaacg atggaatctg tgtcactctt
    30961 ctatttaaat ggcaaaaatt tcaatcttta aatgacaaaa accacctgat ttttggtggt
    31021 tttttatata cctatgacta aactatctct tgctgcttcg acaacttcta cagtgatctt
    31081 atccatttga ttgattctta aaatccgatc aatctgcgca aataaacgat gaataagcct
    31141 aaaatttcct tttgtgatct taatgatagt tgtgatagcc tcataatcag aaaaatcttc
    31201 tagcttgata tcaattccta aatctgccca tttgtattcc aaaatatgat gtgtttcgtc
    31261 cttactaagg ttatcaaatt catgtgcaaa tcctatgcga gagtaaagct gcggataacg
    31321 agacaatctt ttttcaatac caggcatacc gataagaacc attgccagat catttctatc
    31381 gtaaatatct cttagctgtt ctaaatgctg aagctttaaa cgatcaattt catcgacaat
    31441 aattaagtca attgcttcat aggttttagg cacaggagga tcttcttgat tgtattttag
    31501 taccatatgc attgctttag tcagacctat tctactagtt attgtgttaa tatccgaact
    31561 gacacgagac atcttttcag ctggtacagt ataaaaaact ttatttacac ttaatatttc
    31621 atctgttgca tttatcaaga acaagaaaaa gtctgaatat tcaaaaaata acggtgtaca
    31681 tttttaaaag gagatgtata atcgagattt agaaagcgtt ttcctaaaaa tttaatgaat
    31741 taggaagtga ttactatgaa aaaattattt agcgcacttt ttattctgtt atttggcttt
    31801 attggtattg ggacttctgg aaatatagtt aaagctagca cgatggttga acaaacaagt
    31861 gaaatggcaa aaacttttag tcaattaaca ccagaagaac aaagttactt caaatccaaa
    31921 ggatttagtg ataaagatac tttttacact caaaagatag agcctcaaga tccgaaaaca
    31981 cgtttttctg caaatgtatt gcggttaacc gcatctacta aaaaaattac taatacgcag
    32041 gggcggacag aatatattat aacaagtccc aattcggcga tgtatcgtat ttctacacgc
    32101 ttgagtttag gtagtgcaaa atcatatact caaacagtaa atctagctaa ttcttaaggt
    32161 tatggtggag gaatgtattt tacatttact gggaaaagac aatattacag tgtaaaatta
    32221 gctatgcaag tttataatac atttggtgta gggtcagtct cttgcactgc gggaggattg
    32281 acaataggaa aatgataatt tcttactatg tcttaaatag aagtgatgat gaaggactct
    32341 tcgtttttca atcattacat gaggatacga tgctaaaaga gttttataat cggattaaga
    32401 ataaacagat agaaacaaat ttatattttt taagtgactc tatcttgcct tttattagca
    32461 accctaaaat tcttaacttg ttgcagacaa aagatttgaa aagtttaccg attattgtca
    32521 ttgatgagga aattgtaaaa tatggcgctt atctaagcgt agaagaatta gaaaaattaa
    32581 ctaacattgg aatgagtatt caggaggata atggggtaca tggaacatag aatgaatatg
    32641 cacagttgag aacggaattt gacatttatt tagaccaaca gggtataccc tgttggtctt
    32701 tttgtgcgtt catga
//
LOCUS       DAXWLF010000013        30603 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXWLF010000013 DAXWLF010000000
VERSION     DAXWLF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.013"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(193..1776)
                     /gene="abc-f"
                     /locus_tag="ACGE4U_000449"
     CDS             complement(193..1776)
                     /gene="abc-f"
                     /locus_tag="ACGE4U_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFF8392639.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(1881..2009)
                     /locus_tag="ACGE4U_000450"
     CDS             complement(1881..2009)
                     /locus_tag="ACGE4U_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392640.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(2354..2497)
                     /locus_tag="ACGE4U_000451"
     CDS             complement(2354..2497)
                     /locus_tag="ACGE4U_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFF8392641.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2720..3199)
                     /locus_tag="ACGE4U_000452"
     CDS             complement(2720..3199)
                     /locus_tag="ACGE4U_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8392642.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3272..3556)
                     /locus_tag="ACGE4U_000453"
     CDS             complement(3272..3556)
                     /locus_tag="ACGE4U_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392643.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3634..3828)
                     /gene="ssrS"
                     /locus_tag="ACGE4U_000454"
     ncRNA           complement(3634..3828)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACGE4U_000454"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3900..5186)
                     /locus_tag="ACGE4U_000455"
     CDS             complement(3900..5186)
                     /locus_tag="ACGE4U_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFF8392644.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5324..5995
                     /locus_tag="ACGE4U_000456"
     CDS             5324..5995
                     /locus_tag="ACGE4U_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFF8392645.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5961..6449
                     /locus_tag="ACGE4U_000457"
     CDS             5961..6449
                     /locus_tag="ACGE4U_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFF8392646.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6462..7409
                     /gene="prmA"
                     /locus_tag="ACGE4U_000458"
     CDS             6462..7409
                     /gene="prmA"
                     /locus_tag="ACGE4U_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFF8392647.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7410..8174
                     /locus_tag="ACGE4U_000459"
     CDS             7410..8174
                     /locus_tag="ACGE4U_000459"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFF8392648.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8407..10620
                     /locus_tag="ACGE4U_000460"
     CDS             8407..10620
                     /locus_tag="ACGE4U_000460"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFF8392649.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSNLKSIVDKIKAVPDVYSVRRTNG"
     gene            10631..11077
                     /gene="dtd"
                     /locus_tag="ACGE4U_000461"
     CDS             10631..11077
                     /gene="dtd"
                     /locus_tag="ACGE4U_000461"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFF8392650.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11248..12507
                     /locus_tag="ACGE4U_000462"
     CDS             11248..12507
                     /locus_tag="ACGE4U_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFF8392651.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12596..13543)
                     /locus_tag="ACGE4U_000463"
     CDS             complement(12596..13543)
                     /locus_tag="ACGE4U_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8392652.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13937..15229
                     /gene="hisS"
                     /locus_tag="ACGE4U_000464"
     CDS             13937..15229
                     /gene="hisS"
                     /locus_tag="ACGE4U_000464"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFF8392653.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15244..17013
                     /gene="aspS"
                     /locus_tag="ACGE4U_000465"
     CDS             15244..17013
                     /gene="aspS"
                     /locus_tag="ACGE4U_000465"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFF8392654.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            17122..17997
                     /locus_tag="ACGE4U_000466"
     CDS             17122..17997
                     /locus_tag="ACGE4U_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8392655.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18263..20146
                     /locus_tag="ACGE4U_000467"
     CDS             18263..20146
                     /locus_tag="ACGE4U_000467"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFF8392656.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20221..20505)
                     /locus_tag="ACGE4U_000468"
     CDS             complement(20221..20505)
                     /locus_tag="ACGE4U_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFF8392657.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEDAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20495..21229)
                     /locus_tag="ACGE4U_000469"
     CDS             complement(20495..21229)
                     /locus_tag="ACGE4U_000469"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFF8392658.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21358..21981
                     /locus_tag="ACGE4U_000470"
     CDS             21358..21981
                     /locus_tag="ACGE4U_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFF8392659.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            22173..23294
                     /locus_tag="ACGE4U_000471"
     CDS             22173..23294
                     /locus_tag="ACGE4U_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFF8392660.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23301..26429
                     /locus_tag="ACGE4U_000472"
     CDS             23301..26429
                     /locus_tag="ACGE4U_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8392661.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26448..29642
                     /locus_tag="ACGE4U_000473"
     CDS             26448..29642
                     /locus_tag="ACGE4U_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFF8392662.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29788..30495)
                     /locus_tag="ACGE4U_000474"
     CDS             complement(29788..30495)
                     /locus_tag="ACGE4U_000474"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8392663.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         9984 a         5573 c         6504 g         8542 t
ORIGIN      
        1 atgtaaaaag aaaaaacatt tttagatcag cctcgtgggc tgtcgacttt attcttcgct
       61 ttatgtttag ctagaaaacc gatccgaaac attcattgat cgaataataa aaacttgatt
      121 ttccacctgt actttgcctg aatcgtgaaa gcacaggtat gaaaatcaag ttttttgttg
      181 cgaaaaaacg tcctagtcaa tcagttggcc gagcggctgg cggctgatct gctcctgatc
      241 aataacgagt gcttcttgga agtgttgctg caaaaagtag cggtcatggc tgactgctag
      301 gagcgttcct tgatagtctt gcagcatttc ttcaatttct tctcgagcat agctgtcgag
      361 gtgattggtt ggttcatcca aaatcaggac gttgattttt tctaaaaaga gtttcagcaa
      421 cgccaaacgg attttttcac caccggataa atctttgatc cgtttaccca catcgctttg
      481 ataaaagcca cttttggcta attgccgtcg agcgacttct tcgtttccag tcaatgtgat
      541 ggcgtaagcc agcaaacggc tatcgggatc ggcaaactgc agctgctgcg gcaaataacc
      601 gatttgcaag ctactaccta atttgatggt gccaccgtaa ggcttttcct caccgagtaa
      661 taatttgagt aaggttgatt ttcctgcacc attgtcgccg atgatggcta aatgatctcc
      721 acgatagact gcaaaactgc tgtctttgaa tagtacttga tcagcgtact gctggtgcaa
      781 gttttgaatg atgaaaactt ctttgccaga ttttccacca ttagacaagc tttgcagccg
      841 atttttcggg ggaggtggcg gtttgacgag tgtcagtttc gccaggcggc gttccagttc
      901 tttggctttt ttgaagaagg actcattgtc tccttcatgt gcccactgac gaaaacgacg
      961 aatcatgact ttcaaccgct ggacttcttt ttgctgcagt tcgtaatttt ttgtcagctc
     1021 agccaagcga gcttctttca agactcgaaa acggctgtag tttccgggat actcaatcaa
     1081 tgcaccttct tccagttccc atgtttttgt ggtgacttga tcgagaaatt gccgatcatg
     1141 agaaacgacc agaaccgatt tttttgtatt ctgtaaatag ttttccaacc agcgaatact
     1201 gttaagatct aaatgattgg tcggttcatc gagtaaaagt aaatcggcat cggctgctaa
     1261 aattttggct aaagcgactt taactcgttc gcctccgcta agttctgtta accgtgcttc
     1321 ctgacggtgt ggaatcccta agccttgcaa gatcgctgca atccggtctt ctaatacgta
     1381 accgcctagt tcttcaaagc gatgctgcag ctcaccgtaa cgggccagac acttctctaa
     1441 gtttgtatct ggttctgtca tcaatgcttc gtagcgactc atttgctgtt tgaggtcctg
     1501 tatctcagcg gcagaatgga gcaagtagtg gttgacggtc gcttcggaaa ctgtaagttc
     1561 ttgttcgacc gtcccgatct ttaagccttt tttgcgactg ataacaccgc tgtcgacacc
     1621 ttctctgccg gtcaagattt gtaaaaaggt tgatttgcca gttccgttga taccgactaa
     1681 ggcaattttt tcaccagctt caatttgaca ggataaatcc tcaaataatg gattcccgcc
     1741 gattgttttt ttaatagaaa ttgcttgtat gatcatgatc tctctccttg agaccactca
     1801 aaaaggccat ggttatccat ggcctgtgtt atttaaactc agattatcat gagaaaaata
     1861 ccttgagatg gccattgatt ttacgtgaat tcaactgttt tggacaaact tgtcccgtca
     1921 acagcaactg taaaaccaat tgcatggcta aatgcgatat ggtatcgttg gtaattttca
     1981 ttacttgtta ttttagtcat gcgcatcata gtattttccc tccttcgtgt agttgtcttt
     2041 tccagtgtac ctgaaagtgt cagaaagttc aactagcgct gacatttact taatatttat
     2101 cgatgtgtaa ttctgtcaga gagctttttt tatctgactg agggtaatgt ctcgtggacc
     2161 acatcattgt attctgagct aaaccagcgt tcaggcgaaa tcatatggtg tttgataaaa
     2221 aactttgtct acatgacttc cttttgccat ttgcttaata ccgttgcatt aaacagctct
     2281 tctctttgga aatctcacca acatcgcgtt caggagtatc gttgtggttg caatggcaag
     2341 cggtagcctt tcatcaattc ttgaatgatt taccgaatgt agcacttgcc ttctcgcggt
     2401 acccgaaccc gaaataaaat tttagtttgg cgataaaatc ggtatccgcc atcgatactt
     2461 catatttttt cgtatcagat tcatcggaaa cttccacatt gacgaaccaa taagctttgg
     2521 catgcttagg tcgttttaaa ccacaccagg gcgactaatc gccctggtgt tcactttttt
     2581 ttattttact tgaactcatt gactgttttc cctttatttt aaacattata aagaccgatg
     2641 agtgcttgcc gcgtcttttg tttttatcac ggttttttgt gcaaaaaaac ctgccgatag
     2701 ttggcaggtc gtctacgtgt taagctttgg tgtggtcttt agctggcaat tggttttcta
     2761 aatcagttcg gacaacaagc acatcgcaag ctgcgtttct aataacatat tcagaaacag
     2821 aaccaataaa taaacgttct acagcattca atcctgtggc tcctaacata atcaaatcta
     2881 cttgattatc ttctgggatt tctctggcaa taatttgttt tggtgagcca tattctacga
     2941 cagaggttac attattcaag ccagctttct tcgcattgct ttcataatct gccaatgttt
     3001 gctttgccat ttcagtagct tgttctgcaa gcattccatc aaaggatgaa acggtttgga
     3061 aagaacgtgt gtcaatgaca tgtgctaata gaagttctcc gttatttcgg atagctacat
     3121 tgaccgcctt tttaaaagct aattcagctt catcagaacc atctacggca accatgattt
     3181 ttttgtattg ttgtaacata agcattcact cctcaagtca atcgtattgt actcttttat
     3241 tgtagaacac tttagggaaa aagagaatta tttactgctt tggttcagca attttttttg
     3301 caaatcggat actgaaggtg gcagaaacaa taattacgat cagtaaaaag accaaggcaa
     3361 taaagcgttg attgaaaaaa gctgttaaaa tacccattac gcctaaaaca gcatatgcag
     3421 ctccgtaaaa ttgtaggaat ccttgatttt tatcattttt gttgatcaat acaaaaaaga
     3481 tatcctgttt attaaataaa taatagctaa cgaataataa tactaaagtg gcgataatca
     3541 ttaaaatcct aatcatgaac aaatcctcca aaataagcaa caaatagaaa aagcaatttg
     3601 ccaagagcaa attgctttat tctttaaaag atggtaatct ccgttaatgc cgttgtttgt
     3661 cctgtagtat tgatcccgcg aattcaagca ggtgggtccc atggtcgtag cttcgaacta
     3721 ccaaatgata aggcatgtta ccagcagtga cacaagacgg gtcccaagat atcaataaaa
     3781 atgttcggtc aacacacaaa gaggacaatt cgaacatcac agatttccca cctctatagt
     3841 agcatatgaa tttaaaaaga gcaatgagag agctcgcaga aacagaaccg ttttcttgtc
     3901 tagttttttg gaggatgctt tttccactct tttatacggt aatactgttg acccagagcc
     3961 tgttcatatt tccctgtatc ttttggctga taatactgag cattcttcag tttatctggt
     4021 aaatattgct gattgaccca agcctgatca aaatgatgag gatactgata acccacgcca
     4081 cggttgagtg atttcgcacc tttgtaatgg ctatctctca aatgatcagg aacatctcca
     4141 gctttgccct ctcgaatatc agcaagtgct gcatctaatg ccatgtaggc agaattggat
     4201 tttggcgaca agcaaagatc gacgacgacg tctgctagtg ggatacgtgc ctcaggcaat
     4261 cccaattttt cagcagctaa tacagcgttc actgtacgag ccgcagcagc aggattacct
     4321 aaacctatat cttcataacc gataaccatc agacgccggc aaatacttgc cagatcacct
     4381 gcctcaacta aacgggcgag ataatgaagt gcagcatcca catcacttcc acgaatcgat
     4441 ttttgaaaag ccgaaataac gtcataatga gcatctccat ttttgtcatg ggtaagagct
     4501 tttctttgga cacattcttc aatgatcgac agtgtaagat gaatccttcc ttctttatct
     4561 gaaggagtcg atagtgtagc cagttccagc ccattcaatg cactccgcaa atcgccgttc
     4621 gttgcacgcg aaaggtgaag aagagcatct tcgtctaact ggattgcttg ttgtccaagg
     4681 ccgcgttctt tatcccttaa agcatgttcg actgccaact ggatgtcttg ttctgttaat
     4741 ggtttgactt caaagatctg tgtacggctg cggatcgctg gattgatggt aatataagga
     4801 ttctcagtag tcgctccgat caagatgatt cggccacttt ctaaatgagg aagcagaaaa
     4861 tcctgttttg ttttatctaa tcggtgtact tcatccaata agaggatcac tgtaccactc
     4921 atttttgctt cttccgctac aacttgtaga tccttttttg aatctgtagc ggcgtttagc
     4981 atgcgaaatg cataattagt agagccggcg atcgcacttg cgatactcgt ttttccagta
     5041 ccaggaggtc catatagaat catggaagag agcatgcgag catcgaccat gcggcgtatg
     5101 atttttcctt ccccgactag atgctgctga ccaacgactt catcgatcgt tcgaggtctc
     5161 atgcgataag ccaatggctg ttgcatagga ttttcctcct tcttgttttt acgtccctta
     5221 attatatcat atttagcaag atgcctagaa agaacagcct tctgttgttt tgttttcata
     5281 gcttatccaa ctaatattgt gtatgataaa gtaggtgaga aaaatgacgg ataaacaaaa
     5341 gatagatgaa tttttttgga ataaaacgac gccagaagtg gcacgggatt tattggggat
     5401 gtatttggag catgacacac cagaaggaag attagctgga tatattgtag acgcggaagc
     5461 atacctaggt ccagaagacg aggccgccca tagctatggt ttaagaagaa caccgcgtgt
     5521 gcgggcaatg tatgaaaaac caggtacgat ctacctttac acgatgcata ctcatcggat
     5581 tttgaacatc attactcagc cagaagggat tcctcaagga gtaatgatac gtgcaatcga
     5641 acccgccgca atgattgatc aaatgagtaa aaatcgtggc ggaaaaactg gtccagatat
     5701 cagcaacggt ccgggaaaat tagtggaagc attagcaatt cctcaagaac tttatggtca
     5761 gtctatagca gatagttcgt tacgcttagt ttttgagaaa aaaaagacac caaaaaagat
     5821 caatgctttg cctagaatcg gtatcccaaa caaaggtgta tggacagaaa agccgttgcg
     5881 ttttgttgtg tcgggaaatc cttatatctc tttacaaaga aaaaaccaaa tcgagaaaga
     5941 ttggggttgg aggaaagaaa atgaaaaaga aggatctatt aacatattta gatgaaataa
     6001 ttgaaaaaca agtgacagac tatgatgtag ctttagattg ggatacaaaa aatcatacga
     6061 tcgagatcgt tgttcgcttg tttgcagaaa ataaagcaca atttgagatt gatgatgcag
     6121 aagggatcgt ttcgcaagaa ccaattatcg aatttgaaga cggcgtactt ctttttaatc
     6181 ctcagaaatc tgtttttgat gaacgcaatt atctagcagt aatcccttat gaaggaaaaa
     6241 aagggttggc aaaatcagta gctgattctt tagtggaata tctaaatgaa gtattagctc
     6301 aagggcaaag cgatttgctt gattttttgg atgaagacga tgacgaagct gtattcgagc
     6361 ttcactggga taatcagcaa ctagcagaat tagtagaagt aaaacagcaa aatgaagcag
     6421 atacttattt accgtaccca agttattaag gagtgacaga tatgaaatgg aatgaagtaa
     6481 aagtagaaac tgaaagtgaa gcagtcgaag cagttgctaa tattttgatg gaggcagggg
     6541 cgagtggtgt cgcgattgaa gattctttag atgttgaaca ttttcagtcg gatcaatatg
     6601 gtgagatttt agataaaaat gattttacat cgctgaaaga aggcgcacta gtcatggctt
     6661 attttccgga aacgactttc ttaccggaaa tcttgccttt tatcaaagat cgtatcacac
     6721 aattacctga gtttggtcta gctattggaa aaaataaagt aacagttagt gaagtagagg
     6781 aaagcaactg ggcaacagcc tggaagaaat actatcatcc ggtgcgtatt acgcgaatgc
     6841 tgacggttgc gccaagctgg gaaaactacc agacagaaga tcctctagaa aaaattatca
     6901 cactagatcc tggtatggca tttggcacag gcactcatcc cacgacttcc ttgacattga
     6961 cagcactaga aacagtcttg cgtggtggtg aaacattgct agatgtggga actgggtcgg
     7021 gcgtgctaag tattgcagca aaatatcttg gtgcaaaaga tgtctatgcc tttgatttag
     7081 acgaagtagc cgttcgttcg gcgaaagaaa atatggatat gaatgaaata gccaaagatg
     7141 tccatgtatc agctaacgat cttttgacag gaatcgagat tgaaagtgat gtaatcgttg
     7201 cgaacatttt agcagatatc atcttattga tgatcccaga tgcttggcgt ttactgaagc
     7261 aaacaggcac gctgatcgtt tcaggaatca ttgaagagaa gaagcaaatg gttctggatg
     7321 ccatgttaga acaagggttt atcgtggatc aaattttcca gcaaaaagac tggtatgcaa
     7381 ttattttgaa aaaaccagag gtggaataga tgcagcggta ttttttaaaa gaatcatatg
     7441 aatcaaaaga ctattttgtt ttagaagggg aaaactatca ccacattgta cgggttatga
     7501 ggatggaacc cgaggatgaa gtttttttgg cattcaatga tcaaatagcc attgttgcag
     7561 aaatcacgga gattactgaa gaggctgttt atctgaaaga aattaaaaaa gaagaaaatc
     7621 aaaaggaact gccaagttcg attaccatag cgagtggcta tcctaaaggg gaaaagctgg
     7681 attggatcgt tcagaaatcc actgaattag gagcgcaagc ttttcttggt ttcccggcat
     7741 cagcctctgt cgttaaatgg gatgcaaaaa aacgtatgaa acgacaacaa cggcttgaaa
     7801 agattgctca agaggcagcg gaacaatccc atcggcagat cgtgccatca gtagagttat
     7861 tggaaacgaa acaagattta tttgagcggc tagatgatta tgacgtggtc ttggttgctt
     7921 atgaggaatc agcaaagcaa ggcgaaaaat caaatgcagc caaaattctt agtgaaacaa
     7981 aaccagatag tcgaatctta gctgtctttg gaccagaagg tggattatcc cgcgatgaag
     8041 tcgattattt tgtagaacac aaagggatac tttgtggatt aggaccaaga atccttcgta
     8101 cagaaacagc acctctttat ttattaagtg ctgctagcta tcaatgggaa ttaatgaatc
     8161 cttctttcag ctaacgttct tcattaataa aagagtatcg acagaaagaa ttgaaaaaat
     8221 agtcaacaat ttgtataata gaaacaatta tttatacaat ggatacgaaa aatcgtttga
     8281 aaaagctttt aaaaagcgaa catttccgta aaaaaacgta taactgcacg gattttggga
     8341 caattatcaa acagatattt gtcccgattc ccgttttacc gaaaaaagaa agggggatcg
     8401 attcctgtga caaaagaaga aattttaacg ggacccggtg tcataaagct cgttagtcaa
     8461 tacatgggtc ccgaacatgt agcatttgtt gagaaagcat gcgaatacgc gactgctgca
     8521 catgacggac agtttagaaa atcaggcgaa ccctacatta ttcatcctat ccaagtcgca
     8581 ggtatattag cagatttaaa aatggatccc catacagtgg ctacaggctt cttacatgat
     8641 gttgttgaag atacagaaat cactttagaa gatctgagag aagaatttgg cgatgacgtt
     8701 gctatgttag tggacggcgt aaccaaatta gggaaaataa aatataaatc ccacgaagag
     8761 cagctagcag aaaaccaccg aaagatgctg ctagcaatgg ctcaagattt acgagttatc
     8821 atggtcaaat tagctgatag actacataac atgcgtacgt tgaagcacct gcgagaagat
     8881 aagcaaagaa ggatcgctca ggaaacttta gaaatctatg cgcctcttgc acatcgtcta
     8941 gggatcagcc ggatcaaatg ggaattagaa gatacagcac ttcgttatct aaatccaaaa
     9001 cagtattacc gtatcgtcca cttgatgcag acgaagagag aagaacgtga aaaatacgta
     9061 agtggtactg ttgaagatat tcgaatagcg acagaagagc tggggatttt tgcagaaatc
     9121 tatggacggc caaaacacat ttattcaatc tatcgtaaaa tgaaagatca gaaaaagcag
     9181 ttcaacgaaa tttatgactt gctagctatt cgagtgatcg tagattcaat caaggattgt
     9241 tatgctgtat taggagcaat ccacacaaaa tggaaaccaa tgcccggcag atttaaggat
     9301 tatattgcca tgccaaaagc taatatgtac caatctttgc atactaccgt gatcggtccg
     9361 gcaggaaatc cggtagaaat tcaaatccga acacaggaaa tgcatgaaat cgctgaattc
     9421 ggggttgctg cacactgggc ctataaagaa ggaaaaaatg aaaaagtaga accagatggt
     9481 atgacgaaac aattaagctg gttccatgag atactcgaac ttcaagacga aagctatgat
     9541 gcttctgaat ttatggaagg cgtaaaagga gatatcttta gtgataaagt ctacgtcttc
     9601 acaccaaaag gagacgttac tgagttacca aaaggatccg gaccattgga ctttgcatac
     9661 agtatccata cagatatcgg taacaaaacc actggtgcaa aagtaaatgg caaaatggtg
     9721 cagcttgatt acaaattgaa aaacggagat atcattgaga tcatgacttc tccaaattca
     9781 tttggcccaa gtcgcgactg gttgaaatta gttgctacta gcaaagcaag aaataagatc
     9841 aaacgtttct tcaaagccca agatcgagaa gaaaatgtga tcaaaggcca cgaatccgtg
     9901 gtcaaatgta ttacagatct aggatttacg cctaaagata ttttgacgaa gaacaaactg
     9961 caagaagcac tcgatcgttt taattatcaa acagaagatg atctctatgc agctgtaggg
    10021 tatggagaag ttagcccctt gacgatggcc aatcgtctga ctgaaaaaga acgtaaagaa
    10081 caaaaaatcg agcagcaaaa gcaagaagca gaagaaatca tgaatcagcc gaaaaaagaa
    10141 cctgacaaaa tgaaagtacg tcatgaaggt ggcgttgtca ttcaaggggt agaaaactta
    10201 ctaattcgta tcagtcgctg ctgtaatccg attcctggtg atgatatcgt tggttatatc
    10261 actaaaggca gagggatatc cattcatcgt cgagattgtc cgaatgttca gcctgacaaa
    10321 ccaaatgtag cagaacgttt gattgaagtc gaatgggaag atacatcgaa tacacgaaaa
    10381 gagtatgatg cagatttgga aatttacggc tataatcgtt caggcttatt gaatgatgta
    10441 cttcaaacag tcaatgcgct aacgaaaaat ctcaacagcg ttgaagcacg gacgaataaa
    10501 gataaaatgg cgacgatcca tttgacggtt ggtatccaga atttatccaa cctaaagagt
    10561 atcgtggata aaatcaaagc agtacctgat gtctacagtg tacgccggac gaatggatag
    10621 gaggacagat atgagagcag ttgttcaacg ggtaagtaaa gctgctgtgt cagtcgatca
    10681 gcaagaaatt ggaaaaattg gctacggcct acttgtttta ttaggcattc atgaaaccga
    10741 tacgcaaaaa gatgtggact atctggtaaa aaaaattgca caaatgcgta tttttgaaga
    10801 tgaacaagga aaaatgaata ttagtgtaga agatgtggga ggacaaattt taagtatctc
    10861 tcaattcaca ctgtttgcgg atacgaaaaa aggaaatcga ccaagctttg tggaagccgc
    10921 acgaccagaa acagctattc ctttatacga atcattcaat gaaggaatca gaagtcgcgg
    10981 catcacagtc gctaccggac aattcggagc agatatgtcg gtttctctcg tcaatgatgg
    11041 acctgtaacg atcattattg attctaaaaa caaataaagt aatctggttt tcactagtaa
    11101 aaactatgaa acacgtcgct taagttagac tgataaactc taacttaagt ggcttttttg
    11161 tctgcaaaaa gcgcttcttt tgtcagcagt cggaaaaaaa gttaagctta aattatacaa
    11221 gttatttttt ataggaggtt gctgaagatg aaagaagtac cagtagtaga tatcaaaatc
    11281 acagatttaa aaaaattgta tcaaaaagga tacaacatgt tggaggagtt acgccatgaa
    11341 gcggacgctc ccgtcgtcaa agcaaagatt ttcaataaag aagctatcac gatctacgga
    11401 tcatcagcag caaaagtatt ttatgatcca aggaatttca aacgaaaagg tgctatgcca
    11461 aaattagtat tgaagacact ttttggtcaa ggtggagtac agactttgga tggagctgct
    11521 catcaccatc ggaaaaatat ttttatggat ctaatgacac cggaaagaat ggaagattat
    11581 catcgtatct tagataagaa tctgacacaa gcacttgaag cacagcatgg tcaatttgaa
    11641 ctatttgatt taagtaagat ggttttcttt acatctattt gcgagtgggc aggtatcaat
    11701 ttatcagcga tttctaaaga tgaggtcgaa aaattagcag aatatcaaat ctctatgatc
    11761 agcggaacgt ttacttctcc gattgatcac ataaaaggtg tcgaaaatcg caaaaaatca
    11821 gaaaaatggg cgcaaggctt gattgaagaa gctcgacaaa atcctgtggc tggaaaagaa
    11881 aatgtggcat tatatgcttt tgccaatgcc acagatttag atggtcagct tttacctctg
    11941 gaagtggcag ctgttgaatt attgaacatc atccgtccaa cggttgctct aactgtgtgg
    12001 gcggcattga tgggacatgc acttttcagt agaccagatt tgtatcagca attaaaaaat
    12061 gacttttcga cgttacaaga tccgtttatt caagaaatga gacgctatta tcctttcttc
    12121 ccaatgctac ctgcgatttc gctgaaagaa gtagaagtag atggttatcg tataccagaa
    12181 ggcagttggg ttattttaga tttatatgga acagaccatg atgaacgaac agttgaagcg
    12241 ccagattcct ttatgatcaa acggtatgta gggaaagcaa aagatatttc ctataaagaa
    12301 gaatatgaga tgattgcaca aggcggggga aattttcgac aaatgcatcg ctgtgcagga
    12361 gaatggatca ctcttcatag tctacgtgtg ttttccgatc agctagtaaa taagtttgag
    12421 ttctctgtac ctgaacaaga ttggacgatt ccattcaatc agtttccgac ttatccgaat
    12481 agccgagcgc tactttacaa gaattagatt agcataaact tatcgaaaaa aaggaagatc
    12541 aaacagtgct gatcttcctt ttttcatatt actataaatt gataaggtca attgatcaaa
    12601 atccacgaaa tgcttcatca atggtttgat aatcttcatt ggataaatgc aaatctaaag
    12661 cttggacatt gtcagatact tgctctggac gcttagctcc aggtataact acagacactc
    12721 gtggattctt catgtaccaa gctaaaacaa gttgcgcagg agtggcttgg tatcttttag
    12781 caatggggcg aagtacatcg acagcggtta aaatttctct gaaacgttct ccttggaaat
    12841 ctggatttct ttttcgagga tctccttcac caaattgttt ttcctcacca agttcatatt
    12901 ttcctgtcag caaaccggaa gcaagaggaa aatagggaac aaaactgatt ttgttttttt
    12961 caagataagg gaataattct ttttcagcct gccgatggat caaactgtat ttatcttcta
    13021 ctacatcgac atatccatct gcattcgctt ctttcaattg ttctaacgtg aagtttgaca
    13081 caccgactgc tcggattttt cctgcttctt ttaattcgtg caatgtagca actgattcat
    13141 tttttggtgt gctttcatcc ggaaagtgga tataaaaaat gtcgatgtaa tcagtttgca
    13201 gacgttttag tgcatcttcc accgattgtt tgagaaactc aggagaatta tcaaatgttc
    13261 gccctttatt tggtacatga gcagcttttg tagcgatgac gacacgtgag cgatcatatt
    13321 ctttttcctt cagtacctcg ccaatcagtt cttcggaacg gccgtttccg taagcataag
    13381 cggtatcgat caaattgatc ccgctattca aagctgtacg aactacttgt tttcctgtct
    13441 catcttccaa accgtcaaaa agattatgtc ctccaacggc atttgctcct aatccaagct
    13501 gttctgcgta gacttgtgaa tgtccgattc ttatttctgc catagctgat tcctcctttt
    13561 tgtctagtct aaaacaaaat accaaaaagc tgaactaaaa cgtttttgtg aggggaaata
    13621 gtcagatatc tcttgacttt gagactggtt tttagtaggt tataagtaat atcgaatgtc
    13681 aatgaaagag cgagtaagtt tttgaaactt tacaagagag gatcatcgag gctgaaaatg
    13741 atctaaagtt tcattagcct aaagacactc tggagatgga cgagtgaaaa gccgtcccgc
    13801 gtacgagcgt tattcgtaag aggaaagaga aatctttcaa aattaggtgg taccgtgtat
    13861 gaatttcatt cacccttgtt catgatgaac aagggtgatt tttttattta catagctaag
    13921 aaagggagag aaaactatga gtttccaaag accaaaagga acaaatgata ttttgccagg
    13981 cgaatcagaa aaatggcagt ttgtcgaaga aacagcacgt ttattattca gagattatca
    14041 gtataatgaa atgcgtacac cgatttttga acactatgaa gtgatatccc gaagtgtagg
    14101 ggatacaacg gatatcgttt caaaagagat gtatgatttt tatgacaaag gggaacgcca
    14161 tgtgacgctt cgaccagaag gtacagcacc aatcgttcgt tcttttgttg agcataaatt
    14221 gttcggccca gagtatgcga aaccgttcaa aactttttat atgggaccaa tgtttcgcta
    14281 cgaacgccct caaaaaggac ggttacgtca attccatcaa atcggagtag aagcatttgg
    14341 atcagaaaac cctgcaactg atgtggagag catggtgatg gcacttgatt tcttcaaaca
    14401 gttggggatc acgcaaatcc ggctagtcat caattcatta ggtgataaag aaacgcgtca
    14461 agcctatcgt caagcattga tcgactatct tttacctttt gaatcagagc tgagtgaaga
    14521 ctctaaacgc cgccttcatg aaaatccatt acgcgtattg gacagtaaag acaaacgaga
    14581 tcaaaaattt gtggcagaag ctccatctat cttagattat ctaagcgagc ctgctcaaaa
    14641 acattttgat accgttgttt ccatgctgga tgcattggct gttccttatg aaatcgacag
    14701 taatatggtt cgcggactag attattacac acatacgatt tttgaaatca tgagtgacgc
    14761 accaaaaatg ggggcacaag caacgatttg cgcaggcgga cgttatgaca atctagtgga
    14821 agaactaggt ggtccttcaa cacctggctt cggatttgca atgggtatcg agcgattact
    14881 cttgactatg gaggcagagg aagtagttat ccctgcattt aatgagttgg atgcttacgt
    14941 ggtcgcactc ggagacgaaa caaatatcga agcattgaaa gtggtccaag cgattcgaaa
    15001 ctttggtttt tcagctgacc gcgacttcat gaaccgtaaa gcaaaagcgc aatttaagac
    15061 agcagataaa gcgaatgcca aattagtatt ggtgatcggt gaagatgagc ttgctagtca
    15121 aacgattaat gtcaaagcaa tggcaagccg aaaagaaaaa agttttccat tagaagagat
    15181 atatgaacat tttgatgaag tctatgatga aatgacttta ttcgagtagg aggaaaaaaa
    15241 taaatggcac aaagaacgat ctattgcgga ttagtttccg aacaacaatt agaacaaaca
    15301 gttacattaa aaggatgggt acaaaaaaga cgagaccttg ggggagtaat ctttatcgac
    15361 ctacgtgatc gtgaaggaat cgttcaaatc gtctttaatc ctgaaatcaa caaagaagca
    15421 tgggaaatcg cggataaatg tcgcagtgaa tacgtgatcg aagtgactgg ggaagttaga
    15481 agacgcggag aaaatgcagt aaatcctaaa atggccactg gtgaattcga agtgattgca
    15541 agtgaaatcg tgattttgaa tcaagcaaaa accccgccat ttcaaattga agatgatcaa
    15601 gcaatcagtg acgaaatacg tttgaaatat cgttatttgg atctgcgtcg tccaaaaatg
    15661 actaaaaaca tcaaattaag aaatgcgaca acaaaagcga ttcgtcatta tttagatgat
    15721 aatgactttc tcgatatcga aacaccttac ctagggaaat ctacaccaga aggcgcaaga
    15781 gactacctag ttccttcacg tgtccatgct ggacatttct atgcgttgcc gcaatctcct
    15841 caattgttta aacaattgct gatgaatgct ggattcgacc gttattatca aatcgttcgt
    15901 tgtttccgcg atgaagattt acgtggagac cgtcagcctg aatttacgca agtagatatt
    15961 gaaacaacgt tcttaacagc agaagaaatc caaacttaca cagagggatt gattgcgaag
    16021 gtaatgaaag aagttcgcgg tatcgaagtc acattgccat ttcctcgtat gacctatgat
    16081 gaagcaatgg ctcgctacgg cagcgacaaa ccggatacac gttttgatat ggaattgatc
    16141 gatctaagcg atacagtcaa agaagtagaa ttcaaagtct tccaaatggc tcttgaaaac
    16201 ggcggtgtcg tgaaagcatt gaatgctaaa ggcgctgcag atcgctactc acgaaaagac
    16261 atggatcagt taggtcaata tgtaggacaa ttcggagcta aggggcttgc atggctgaaa
    16321 gtagaagaag acggattgaa aggtccaatt gctaaattca tgggagaagc aacagaagca
    16381 atcattaaag caacagatgc aaaaccaggt gacttgctga tgtttggcgc agataaaagt
    16441 gaaatcgtcg ctgctgcatt aggtgctatc cggacacgtt taggaaaaga attaggacta
    16501 attgatgaat cgaaattcaa tttcttatgg gtaactgatt ggccacaatt tgaattttca
    16561 gaagaagaag gtcgatatgt ctctgctcac catccattca cgatgccaaa agaagaagat
    16621 atcccgttat tggctgaaga ccctgcaaaa gtctatgcag aagcttatga tatcgtcttg
    16681 aacggttatg aactaggcgg aggttctatc cgtattcata ctcgtgagct tcaagaaaaa
    16741 gtactagaaa ctctaggctt tacaaaagaa caaatggaag aacaatttgg tttcttgtta
    16801 acagctttag attacggatt ccctccacat ggcggtattg cattaggatt ggaccgtttt
    16861 gcaatgttat tagccggaga agataatatt cgtgaagtca ttgcctttcc taaaaacgga
    16921 aaagcagcag atgtgatgag tgatgcacca agtatggttt cgcctttaca attgttcgaa
    16981 ttgaatattg atgtaacgaa tatagaagaa tagccgataa aaaggtaaaa aggatttgtc
    17041 gaatacgctt cttttgacaa ccttgattta attgaaaaac actttataat gaagccataa
    17101 catataaagc ggggcttttc catgaaaaaa acattcgaaa attgggctta tcatgattac
    17161 acgaccaagc tttcggtatc tatcgtttat gcgttgatcg catcagtcgc attgaatttc
    17221 ttttatcagc caggacatat ttattcaagt ggtattactg gtctagctca gattttgacg
    17281 actttgtcaa aatcactgat aggattcgaa gtaccggtat caacaacttt gtatttactg
    17341 aatatacctt tgtttgtatt ggcgtggtta aaaatcggga agaagtttac aattttcact
    17401 tttatcacag tcactttgac ctccctttgt atccatattt taccggaaac agtcctatct
    17461 accgatccaa tcatctgtgc gttgtttggg ggggctgtga tgggactagg tattgggcta
    17521 gcgttgagaa acggcctatc atcgggcggt ctagatatcg tgagtatcac gattcgtaaa
    17581 aaaacaggaa agtcgattgg ttctatttcg atctacttca atgcattgat cgtttttgtt
    17641 gccggaattc tatttggctg gcaatatatg ttttacagta cgttgtctat tttcgtcagt
    17701 ggaaaagtaa ctgatgtcgt ttatactaaa caaaagaaga tgcaggtaat gatcgtaacg
    17761 aaacatccag aaaaagtagt aaatgaaatc caaaactgta tgcgtagagg gatcacgatc
    17821 atcaatgaag cagaaggtgc ttataagcat gataagcaga cagtattgat gacgatcgtt
    17881 acacgatacg aactgcctct acttagagaa gcaatgaaag cttctgatcc acaagctttt
    17941 gtaagtattg cagaaaacgt ccagatactt ggtcgtttct atgaggatgg catttagatc
    18001 gagtttgaca aaaattcatg aaatactgct gtagacagac gttcgtttgt ctgcagtttt
    18061 tttctaccat agtagaatca gcaaagaaaa actgcttttt tatttgaaat tttcttaaag
    18121 ttgaaaaaaa gcatgtttat ttttttaaat ttatgggatg atagaaaaaa atagtagaga
    18181 aaggacacga ttctacttgc tattttcact taatacccgt atattacgaa tgaaaaaagt
    18241 ttatgaaaag ttggaatatt gaatggaaaa acaaaaaagg ttaaaaaaca gccgctacat
    18301 cactgggttt gatgggctcc gttcattagc tgtggtgggt gttatcttct atcaccttct
    18361 gccaactagt atgaaaggcg gctatttggg cgttccgatt tttttcgtcg tatctggtta
    18421 tttgatcacg gatctgctgc ggcaggaatg ggaacaaaat ggaaaaatca atatttggca
    18481 attttatgtc agaagaatga agagactcta tccggcactt gtttttatgc ttgtgacagc
    18541 ttcggcttat atcactttgt ttcagcgagg gctgttaaac aatcttcgag gagttgtggt
    18601 cagcagctta ctgtatgtca acaactggtg gcaaatcaat aacggtttat cttatttcga
    18661 tcgatttgct aatgagtcac cgtttacgca tatctggtct ttagcagtag aaggtcaaaa
    18721 ctatttggtt tggccgatct tgtttgtatt gttgatggta tttgttcgta aaagaaaatg
    18781 gatcttttat actgtgttag gtacatcctt cctctccgcc atcttgatga tgatcttgta
    18841 tacaccagga ggcgatccaa cccgtgttta ttacggtacc gacactcgtc tgttttctat
    18901 atggttggga agtgcgcttg cgtttgtttg gccaagtacg cgtttaaaga aaaacatccc
    18961 gaaacaagcg aaaaatattc tgaatatcgc tggaggaatc tcgcttttgg cactgattct
    19021 ttgcttcttc tttttagatg atcattacaa tttcatctat tacggaggaa tgctcctagt
    19081 gagtctgtta tgcgtagtgc tagttgcagt gacagcacac cccggagcaa gtttgaatcg
    19141 atggctgaca aatccagtat ttacttggat tggcaaacga agctatggca tttatctgta
    19201 tcagtttcca gttatgattt tttatgaagc aaaggtgact gatattgccg atcatgtttt
    19261 gttgcatact ttagtggaaa tcacattgat tctaatcatc agtgaacttt cttatcgata
    19321 tgtggagcgt ccgctagcac gttttgatta tggtcgtaca ttccaagtga tgaaaggatg
    19381 gttcagcaaa ccgatcatca gtaagagcaa accatgggtt gttccaggca cattacttgt
    19441 agcaattgca ctcgttggtt tcgttacggc accgaaaaac gctgtaacag ccgatcaaaa
    19501 acaactgcag gcaaaaatcg ctgaaagtaa aaaattagca gaagaaacca gaaacaattc
    19561 agaaaacacc actagttcgg ttgaccaagc gatccttgat aagtacgagt tgtccaaggc
    19621 acaagggaat aaggcacaaa aattagaact aacggcattt ggtgattcag ttatgttgga
    19681 tgctgcaacg gatctacaag aactttatcc aaaagtagtg gtagatggag atgtcggacg
    19741 acaattatat gccagtgtac cgtacattga aaaattaaag gaacaagatt tgttgaaaga
    19801 cactgttctg atcggactag gaacgaatgg ctcattctcg gaagctcagt ttgatagtgt
    19861 gatggatgca atcggtgatc gaaaagtata ctgggtcaat gtccgagtac cgacgaagcg
    19921 ctggcaaaat gacgtgaatg cgatgcttga acagatgacg aaaaaatacg acaatatgac
    19981 gctgattgat tggtacagct acagtaatga ccatagtgac tggttttatg atgatcaagt
    20041 ccatccaaat cctgaaggga tgacacatta cattcactta gtcagcgaag ccttgctagg
    20101 aggaagttca agtacgaaca gcagtgagac aaacgtgaca gaatgatagc tacaaaaaac
    20161 ttcaggcaaa aacatttgtt tttgcctgaa gtttttttat atttaccgtg tttttaacat
    20221 tcatatagat tcaggaagca aaacggttga gtgtgtctgt aaataacgtt ctttttgttt
    20281 tcgggtgagt tttttgaatg catcttctgc ttttgttgct tcgcttcttg aagaaaatac
    20341 ttctgcatgg atcatcttaa caggtcttct tttttgaaga cgagtatatt tagcaccagt
    20401 accattgtta tgttcctcta atcgtctaga aagatcggtt gtatatccac cataaaatgt
    20461 ctgatcttta cagaataata cgtaaaaata atgttcattt gccatagagc atctcacgaa
    20521 tctctgcagt gtattctcca tccatattgt aagtgatcaa tggtggagcg actttgaatc
    20581 cgtctaattt gccttctttg attccttcaa ctaacaaaat atttgcctct tttccaggtt
    20641 ttgggtaaac aaattggatc cttttaggtg caattcgata gtcttccatg acctgaatga
    20701 tttctaagaa ccgatcagga cgatggacca tagtaaagcg tccatttgtt tttagtagct
    20761 tacttgaaat atcgatcact tcttctaacg tcgtatgaat ctcatgacgg gcgattgcca
    20821 ggtgtttatt agggttcgtt ttatttgttg gcagcccttt gaaataaggt ggattacaga
    20881 cgactaagtc acaagaatta tgtcgaacga cagataagct gtctttcaga tcgatggtgt
    20941 ggacagtcat ttgttcattt aaatggttaa gcgcgatact acgtcttgcc atgtctgcta
    21001 gacgaggttg aagttcgata gcgtcgatag ccgcctgggt tcgtttactt aaaaaaagtc
    21061 caactgcgcc atttcctgca caaaggtcaa caatcttccc ttttttaggt acacgggaaa
    21121 agtgagcaag cagcacagca tctaaagaaa aagaaaacac tttggaactt tggataatct
    21181 tgatatcttc tgcgtataat tggtcgattc gttcgtcttc gtgaagcatg gattccctcc
    21241 aaaatgaaaa aaatagtcgt ctgtattata ctatttttat taaaaagaac caaggtggct
    21301 tgtcaaacct ctatttttta tgcatactat gaccataaga agggaaagga agagactatg
    21361 ttttatcgat ttatgcgcgg ggtagttcga gtactattag ccttagttaa tggaaatgcc
    21421 cgctatgaga ataaagaagt tttaccgcaa gatgagaatt acatactagt tgctccccat
    21481 cgtacttggt gggacccatt atatctggcg gtagctgcag cacccaagga attcagcttt
    21541 atggcaaaag aagaactgtt caaaaatcca gttctgcgat ttatattaac tcgatccaat
    21601 gcttttccag ttaaaagaga aaatccagga ccaagcgtca ttaaaacacc tgtaaagatt
    21661 ttgaaatcga caaatttgag tttgatcatg tttcccagtg ggacaagaca tgctacagaa
    21721 cttaaaggcg gaatggcatt gatttccaga atgtctaaag cgaaaatcgt gccagctgta
    21781 tatcaaggtc cgttgacact tggggatctg ttcaagcgtc agagagtgac agtgcgtttc
    21841 ggcgaaccaa tcgacttatc agatatcaag aaaacggata aagaaggtat ggcggagatc
    21901 gaacgacgaa cacaagcagc ttttgacaaa ttggacaaag aagtgaaccc tgatttcaaa
    21961 tatgaagtca aaaaaaaata aaatgataag tttatgcgtg ggcggtcttt tgaatctact
    22021 gtcctgttca gttcagactt tttcaatgaa aacgagggag agacgtcaag tctgccctct
    22081 ttttgcgttc caatatgtaa aatagaagac aggtgtcatg taatggatca aagtgttata
    22141 ataattagaa ggacattttt tgaggtgatt gaatgcgttt tttacataca gccgattggc
    22201 atatcggaaa aaaactacaa ggttatgact tgattgaaga ccagcgtcat gtactatctt
    22261 ctatactaga tatcgcgaaa gaagaaaaag tagaggcaat cgtgattgcc ggagacttat
    22321 atgataggag tgtcccagct gttgatgccg tagaattata caatgaattg atggtcgatt
    22381 ggaatttaaa ggaaaagatc ccgatttttg ctatatcagg aaatcacgat agcagtacga
    22441 gactttcagc gggtacgcca tggttcagtt attcgaacta ttttttgcga acacagctag
    22501 cagaagcatt tgaaccagtc gagtttaaaa acgtacaatt tttctttctt ccttactttg
    22561 aaccaattgc agcgagatta tattttgaag atgacaatat ccgaacgatc aagcaagcgg
    22621 tagccaaggt gatagataaa atgcaggagc tatttgattc agaaaaaaaa caagtattga
    22681 ttagccattt ttttgtaatg ggaagtgtaa aatctgattc ggaaacgaaa atcgaagtag
    22741 gcgggttgga ttcgatccct ggtgaactac ttacaccgtt tgattatgta gcactaggac
    22801 atttgcataa tcaagctgct ttgagacagg ataatgctcg ctatagcggt tccccattga
    22861 aattctcttt atctgagatc aaccaaaaaa aaggcgtatg gatcgttgac ctgacaacag
    22921 gcctggagtt gaattttagg gagatcgaac ccctgcatga tattgaagaa ataacagcga
    22981 gtttcaaaga attgctggat cctgcctttt atcagtccgt caatcgggag aattacatct
    23041 atatctatct cactgataga acgatcatcc cgaatatgat gaaccagctt cggcaaatat
    23101 atcctcgtat tttggggatt gaacgggcaa acggtcgagc agaacaaaca aagcgcaaac
    23161 gaaaagtact agaaatgaaa gatcctcaaa aattagcaga agcatttttt agtgaaatga
    23221 cggaagagca gctaactgca aaccaagaaa aatggctcgg agaaacgttg caagaaatca
    23281 tggaaatgga atgatcaaga atgaaaccga aaaaattaca acttaaaaat tttggtcctt
    23341 ttatcaatga gaccgtagat ttttcacgtt tgacagaagc accgcttttt ttgatcagcg
    23401 ggaagacagg agctggcaaa acgacgattt ttgatgggat caccttcgca ctttttggtg
    23461 aaacttccgg aagacttcgc tctggaaaag agatgcgttc tttgtttgct acaccagacg
    23521 aagaaacaag cgtgactttt tcttttgaac atcagcagat gagctatgaa atcgaacgaa
    23581 aaccagaaca agttttagca aaacgaaaag gaaacggaac aaaaaaacag gcagccaaag
    23641 taacgttgac catttttgat gggaaaggaa aagaaataaa acaaatcaca aaacgtacag
    23701 aagcagatca gttgatcaga gaactgatga atctcgatgc gaaacagttt tctcaaatcg
    23761 ttttactacc ccaaggagaa ttccgtaatt ttttggtttc atcaagcagt gataaagaga
    23821 ctgttttacg tcatttattc ggtactcaat ttttccaaca gttcaatgag cgattaaaag
    23881 aaaaagcaaa acaacagcaa caaaaattgg atcatttaga gcaagagctg catcttctgc
    23941 aaaaaaggtt tgttttagtc aaagaagagg aaccagcagc agggtttgaa gccgttttag
    24001 cccaatggac aaaccatcag gcaatactcg aaaaacaaat cacggaagaa aaagaagctc
    24061 taacggaact tcaggaaaaa caaaagcaac tagaaaattc ctactatcgt tttgaaagct
    24121 tgaaaaaaag ttatgaagaa aaaacgatat taataaacaa gcaaaaggaa ttagctgctc
    24181 aaaaagacga aatagaggaa aagaaacgat ggattgggta ttatgaattt acgcaacgat
    24241 tagtcgaacc aatccagcgc ctacaagaaa taaaagttga aaaaatagaa ctgtcacaaa
    24301 aaagtagtga gcaaacaaag aaactagcga tgactacgca gaattatgaa gagtggcaaa
    24361 aaaatgaagc aaaccgcgta cgccgacaaa caaaaatcaa agaagctgct caaaaactgc
    24421 aaaacaaaca gatgatggtg ccaattgtcg aatccttacg tagtaaaaaa caagacgcag
    24481 aacaattaac aatttcattg gataaagaag aattgaatgc cgagaagctg tatcaaaatc
    24541 tcgctatcta tcaggaaaga actcgaattc ttcaagaaaa tttactggat caggaaaaac
    24601 tgcaagaaga tcgtcttgct tttggacagt taaaatatct agatgaaaag cagcaagaaa
    24661 tagaaaaaca aaaagcaaaa gacactgggt cgctcgcaca gataagagaa gaactcaaaa
    24721 aagcaactga agaaataaca aaaaatagtc agctattgaa agaacagaca gaacaagcag
    24781 aacagctgaa aagccaatgg gcaaagcagc aaattgctcg tcttcagctt tttttgcgtc
    24841 caggggagcc atgtcttgtt tgtggatcaa cggaacaccc catacaacaa ttaaaacacg
    24901 aacagccaaa taaggaagat atcttaaaag tagaacaaca actggcagaa gctgagtctg
    24961 ctgtagaaag aactcagcaa atcattgcca aaagtgaagc acatgaaaat aatcttcaac
    25021 aacaattatc agatttagaa caaaaaatca tggcaacgaa tatacaattg gctgaacaag
    25081 aagaacactt gcaacaggct ttgaaaaagg catttcctga gataacagaa caaaaaacaa
    25141 aaacaactct tgtagtaatc gaacaatctt tagatagacg aaaaagcaag atggccaatg
    25201 caagaataga gctggaagaa ataagacaac aagcagaaaa gctcgaacaa caattagctg
    25261 tctacaaaca gcagcagaca gaaaaaaata atcagctcca gcaactgcaa ggagaaatca
    25321 aaagtttaga acaacaattg caagatacgg atagtaactg tttagaagag gagatacgac
    25381 gacttgaaaa acagttagaa gaagactcga atcaactagc tgttgagcag aaagcaggag
    25441 tgcaattgtt gcaagatctt catacgcttc ggacacagct tgatcttcaa catgaacaaa
    25501 tagaaaaaat agatgaaaaa atagaaaagg aacaagaaag aatagctaaa aagttagaaa
    25561 gccagtcgta tttttcaaca attgaagaaa tctgtgcatt tgcacaacag caagcacaat
    25621 ataaagaaaa ccaacgtaca gttacttctt atcatgaaga gcaattagtg acagctgacc
    25681 ggctgcgtca attagaaaat atccagctaa ctgagacatt cccagataca gaagagatca
    25741 gccaacagtt agaagaaata agacgtatga tcgaaataaa gcaagaaaag atatacggtt
    25801 tacaagaaca aaaaattagt aatcaaaaaa tctacgatga atgtctttcg atttatcaca
    25861 caagccagac gaaattagat gagcttacgc aattacagca gctttcgcaa acattcaacg
    25921 gagaaaatcc taagaagact agtttggaac ggtatgtgct tcaggtttat cttcaagaag
    25981 tgttacaagt agcaaatgat catctccaac gtttaacgaa aaatcgttat caatttgagc
    26041 tagcggatac cattggcagt tatcgaggga aaaccggatt agaaatcaat atctatgatg
    26101 atcaggcagg tatgtctcga agtgcgcata ccctgtctgg cggtgagagt tttattgcag
    26161 cactttcttt agcattagct cttgccgatg tgatccagac acaagctggt ggtgtagcaa
    26221 tcgatgcatt gtttattgat gaaggatttg ggtcattaga tgaagaagct ttggaaatgg
    26281 caatggaagc tttagaaacg atcgaaagtg aaggaagaat gatcggcatc attagtcatg
    26341 taagagaatt gaaagaacga gtattacaac aaataagaat tgatacagaa ggtagcggac
    26401 aaagcaaaat acgctatcaa cttggataaa aagggagatt ggtaactatg aaatggagta
    26461 ttccagaaaa agtcattgca cgtggacgtc aatacttgca agaaggccga gtgttatcgg
    26521 ttgtccctga ccaagaagaa aacgtgtggc atgcagaagt cttgggcagt gaattgtata
    26581 tggttgattt ggatggtaca gcaaaagaga tagattactg ccaatgtccc tattgggagg
    26641 aacataaata ttgtaagcat acagttgctg tggaacttta tttacggtcg aaaaatctat
    26701 ctcgtatcat gaaaaaagac cagccaatgc tagaaccggt aaaagcaaca agtgagggag
    26761 aaatcattgc aaaaggtttt tctcgtctaa aaacacaaaa caatcctcaa gcggtgcaac
    26821 ctttgattat tgaataccaa gtagaaacga ttgagactaa tcagtatcac ccagagcttt
    26881 ctattttggc actttacttg cgtatcggtt cattggcatc accgaaaaaa acatatatcg
    26941 taaaaaacat ctatgatttt ttacaagctt acacagaaaa agaaacttac actgttaata
    27001 aacagtacca gtttcgtcta gataaacggg cgtttcaaaa agaagatcaa aaaatactca
    27061 cgcatcttgc aggaagtgct cagacgcatc aattattagg tacgaacggc ttacaagtca
    27121 aaggaaaact agataaaaga tatctgcttc tcccagtaga acaagcccgc ttcctgttag
    27181 agcagatgaa tcagactaca cgtttgcatc tgcagataaa tgaacaaaac gatcaaggaa
    27241 tacaattttc cgatcaaaac aaacctttat cattcaaagt ggaaaaattt ggagaagatt
    27301 atttgctgca agcattgaat gattttgatt ttttctttat gcattatcag tggggagtta
    27361 tccaagggac attttattct ctgacgaaat atcaacaaac ggtttatcaa acttggaaac
    27421 aattgattcg ccgattggag aaaccagaag tagtgttcaa gaaaaaagaa ttaccgtctt
    27481 tgtttaaaga gattatccct ctgctgtcag agatcggcga cgtagctatt gcccctgcag
    27541 tagaagagga agtagcagat atccccgtcg aatttatttt ctttttccga aaagcaaaag
    27601 gaaaaatcca agctcgagtc gatttcgtct atggcgaagt catctattcg actgacccga
    27661 agcatgaggt ctcgacagaa caggcttttc aggtgttacg tgacacagta gaagagaaac
    27721 gagtacttga tctattcaga atgtatcgct accagaaaaa tgaaactggt tatgaacgtc
    27781 cattgccaag cggggaagaa ctatatgcct ttttccgcac agaactatct gtcttccgtc
    27841 agtttggtga agtcagactt ggaagaaaat tgcgagagtt gtatttagat gcacataaac
    27901 atcaaccgaa aatcgaagta accgagaccg gttcatggct cgatattcga ttcgatgtga
    27961 cagggataga ggagcaagaa atcgatcatg tcctgcaaag cttgctgaga aatgatgctt
    28021 tttatacatt agaaaatggt caggttcttt cttttgattc agaagaattt caacaaacaa
    28081 gccaagtgtt gcaacaatta cgggaatcga tacgtacaga agaaggaacg atccatgtac
    28141 ctaaaaatca aggattgatc attcaaaatc aattggaaaa aagcaatgct acttttagtg
    28201 aatcgttcca aacgatggta caagatctca ttcatccaga aagataccag gcacagcttc
    28261 ccaaaggatt gaatgcaacg atgagagatt atcaaaagca aggttttcga tggttgaaga
    28321 tgttaggtca ttatcagttt ggcggtattt tagccgatga aatgggactt gggaaaacac
    28381 tacagacaat tgccttttta ctttcggaaa aagaagaacg gaaaagcttc tctgctttga
    28441 tcgtagcgcc tgcgagctta atttacaact ggcaggcaga ggtgagaaaa ttcgcccctt
    28501 ctttaagtat ccaagtaatc aatgggaata aaaaagaacg tgaagaatta ctggctaaag
    28561 acacagatat ccgagtgact tcttatgcca gcttaagaca ggatctagct aattatcagt
    28621 cgcaaaaaat tgattatttg attcttgatg aggcacaaat ggtcaaaaat agcagtacga
    28681 aaactgcaca agcattgaga gaattagctg tacctcagcg gtttgcactg agtgggacac
    28741 caatcgagaa taatttagaa gaattgtggt cattgtttgc aacgattatg ccaggatttt
    28801 tcccgactaa aacaaaattc agggaacttg ccgcagaaga aattgcgcaa atgattcgac
    28861 cgtttatttt acggcgagat aaacaaactg tgttgaaaga cttgccggaa aaaacagaga
    28921 tgaatctata ttctgcactt acagaagaac aaaaaactgt ttatttagct tacttgcgtc
    28981 aaatgcgtga agaaatcaca tcaatggatt ctgaagcatt caagaaaaac cggatcggga
    29041 tacttgctgg tctgacaaga ctgcgacaaa tttgctgcga tcctcgtctg tttattgaag
    29101 attaccaagg cggttccggc aagctcgaac aagtaaaaga tctactggta gctgccaaag
    29161 agaacaaacg aagagttttg ctgttttctc aatttacagg gatgctgact atcttgcaag
    29221 aagagttagc cgagctaggg atctcgacat tttatttaag aggcagtacg aaaccacaag
    29281 atcggttaag tatggtagac gcatttaatg ctggcgaaaa agatgttttt ctgatttcct
    29341 taaaggctgg gggaactgga ttgaatctca cgggagcaga tacggtcatc ttgtatgacc
    29401 tttggtggaa tcctgccatt gaagaacaag ctgctggacg tgcacatcgg atcggacaaa
    29461 aaaatgtggt agaagtgtgg cgtatgatct ctgaaggaac gattgaggaa cgcatggatt
    29521 cactccagca agaaaaacgc gaactgttcc aaaaagtgat ccaaggtaat gaagaacagt
    29581 tgacgaaact gactgaagaa gacatccgca tgattttaag tgtaggagaa atcgaggagt
    29641 aagagactcg aaaaagcgtt cagtaatggg taagaaagcc tttctaaaga agcgattgtt
    29701 tatttgattt aaaaaatagc aaggaggctg ggattccatt ttatcccagc ttccttgcta
    29761 tcattttttt atttctattt ttaaaggtta tacaaagatt gtcttcattg tctctaacag
    29821 ctgatcaaaa gaagaaatct cgatatcgca aattggagtc tgttcaattt tgcgttctcg
    29881 atggttgaac caaagcgctt gccagttagc actttttgca cctagaacat cattgtcgta
    29941 attatcgcca acgtataagg tctcttctgg aagcatatga aattctttct cagctagctg
    30001 aaaaatctct ttttctggtt tttggaaacc tgttgcttga gaaatgagca tattgcggga
    30061 aggaatccaa ttgttcaatt gaagttgctt cagttttttt gtctgatggt cagtcggccc
    30121 atttgtgata attcctaatg ggacattctt ttccttcaag aaatcgaggg tttttttgac
    30181 ctcctcatgc aagcagatat tatcaagttc ttcctcgtaa attttttgaa ataagaggcc
    30241 atcttcttcc gtaatatgag ggtagtctaa atcttttagt gattggctga tccgatgtgc
    30301 tcgcatatat tctagtgtcc aatctcctgc cattactttt gggaagtttt catcactatg
    30361 atggcggaac cggatatata gtggatgcat atctgcatct gaaacaagcg gaacgactct
    30421 tttgaccgcg tttcgaaatg gctgctgctg atcatacatc gtatcatcaa catcaaacac
    30481 aactgattta atcaaaattt tacacgcctt tcggttttca ttgatttttt cacaaatcct
    30541 tgactattgt aacagaaaaa attgtctaaa aaagctgata aataaaggtt tttcttacta
    30601 tat
//
LOCUS       DAXWLF010000014        30234 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXWLF010000014 DAXWLF010000000
VERSION     DAXWLF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.014"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            3..371
                     /locus_tag="ACGE4U_000475"
     CDS             3..371
                     /locus_tag="ACGE4U_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFF8392664.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACGE4U_000476"
     CDS             551..1492
                     /locus_tag="ACGE4U_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFF8392665.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFIALIK"
     gene            1561..2292
                     /locus_tag="ACGE4U_000477"
     CDS             1561..2292
                     /locus_tag="ACGE4U_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8392666.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACGE4U_000478"
     CDS             2436..4616
                     /locus_tag="ACGE4U_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFF8392667.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACGE4U_000479"
     CDS             4636..5118
                     /locus_tag="ACGE4U_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFF8392668.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACGE4U_000480"
     CDS             5134..5895
                     /locus_tag="ACGE4U_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFF8392669.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACGE4U_000481"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACGE4U_000481"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFF8392670.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACGE4U_000482"
     CDS             7667..8152
                     /locus_tag="ACGE4U_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFF8392671.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACGE4U_000483"
     CDS             8145..8828
                     /locus_tag="ACGE4U_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFF8392672.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACGE4U_000484"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACGE4U_000484"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFF8392673.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACGE4U_000485"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACGE4U_000485"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFF8392674.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACGE4U_000486"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACGE4U_000486"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFF8392675.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACGE4U_000487"
     CDS             13125..13457
                     /locus_tag="ACGE4U_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFF8392676.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACGE4U_000488"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACGE4U_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFF8392677.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACGE4U_000489"
     CDS             14283..15656
                     /locus_tag="ACGE4U_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFF8392678.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACGE4U_000490"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACGE4U_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFF8392679.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACGE4U_000491"
     CDS             16711..17151
                     /locus_tag="ACGE4U_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFF8392680.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACGE4U_000492"
     CDS             17295..17996
                     /locus_tag="ACGE4U_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFF8392681.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACGE4U_000493"
     CDS             17993..19114
                     /locus_tag="ACGE4U_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFF8392682.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACGE4U_000494"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACGE4U_000494"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFF8392683.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACGE4U_000495"
     CDS             20493..21401
                     /locus_tag="ACGE4U_000495"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8392684.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACGE4U_000496"
     CDS             21436..21708
                     /locus_tag="ACGE4U_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFF8392685.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACGE4U_000497"
     CDS             21719..22765
                     /locus_tag="ACGE4U_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFF8392686.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23016..25625
                     /gene="clpB"
                     /locus_tag="ACGE4U_000498"
     CDS             23016..25625
                     /gene="clpB"
                     /locus_tag="ACGE4U_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFF8392687.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25776..26462)
                     /locus_tag="ACGE4U_000499"
     CDS             complement(25776..26462)
                     /locus_tag="ACGE4U_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392688.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26440..26934)
                     /locus_tag="ACGE4U_000500"
     CDS             complement(26440..26934)
                     /locus_tag="ACGE4U_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFF8392689.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26954..28174)
                     /locus_tag="ACGE4U_000501"
     CDS             complement(26954..28174)
                     /locus_tag="ACGE4U_000501"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8392690.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28321..30156
                     /gene="lepA"
                     /locus_tag="ACGE4U_000502"
     CDS             28321..30156
                     /gene="lepA"
                     /locus_tag="ACGE4U_000502"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFF8392691.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        10135 a         5386 c         6524 g         8189 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttatagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    22921 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    22981 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23041 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23101 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23161 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23221 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23281 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23341 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23401 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23461 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23521 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23581 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    23641 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    23701 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    23761 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    23821 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    23881 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    23941 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24001 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24061 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24121 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24181 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24241 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24301 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24361 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24421 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24481 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24541 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24601 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    24661 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    24721 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    24781 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    24841 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    24901 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    24961 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25021 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25081 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25141 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25201 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25261 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25321 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25381 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25441 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25501 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25561 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25621 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    25681 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    25741 cttgaagctg cctgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    25801 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    25861 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    25921 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    25981 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26041 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26101 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26161 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26221 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26281 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26341 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26401 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26461 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26521 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26581 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    26641 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    26701 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    26761 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    26821 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    26881 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    26941 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27001 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27061 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27121 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27181 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27241 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27301 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27361 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27421 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27481 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27541 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27601 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    27661 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    27721 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    27781 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    27841 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    27901 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    27961 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28021 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28081 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28141 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28201 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28261 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28321 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28381 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28441 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28501 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28561 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28621 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    28681 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    28741 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    28801 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    28861 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    28921 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    28981 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29041 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29101 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29161 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29221 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29281 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29341 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29401 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29461 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29521 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29581 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    29641 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    29701 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    29761 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    29821 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    29881 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    29941 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30001 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30061 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30121 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30181 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtgg tttt
//
LOCUS       DAXWLF010000015        29668 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXWLF010000015 DAXWLF010000000
VERSION     DAXWLF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.015"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..566)
                     /locus_tag="ACGE4U_000503"
     CDS             complement(<1..566)
                     /locus_tag="ACGE4U_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFF8392692.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKF"
     gene            complement(643..1173)
                     /locus_tag="ACGE4U_000504"
     CDS             complement(643..1173)
                     /locus_tag="ACGE4U_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFF8392693.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1177..2037)
                     /gene="rlmB"
                     /locus_tag="ACGE4U_000505"
     CDS             complement(1177..2037)
                     /gene="rlmB"
                     /locus_tag="ACGE4U_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFF8392694.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2030..2425)
                     /locus_tag="ACGE4U_000506"
     CDS             complement(2030..2425)
                     /locus_tag="ACGE4U_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFF8392695.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2422..3834)
                     /gene="cysS"
                     /locus_tag="ACGE4U_000507"
     CDS             complement(2422..3834)
                     /gene="cysS"
                     /locus_tag="ACGE4U_000507"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFF8392696.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3831..4367)
                     /gene="epsC"
                     /locus_tag="ACGE4U_000508"
     CDS             complement(3831..4367)
                     /gene="epsC"
                     /locus_tag="ACGE4U_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFF8392697.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4453..4692)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4689..6149)
                     /gene="gltX"
                     /locus_tag="ACGE4U_000509"
     CDS             complement(4689..6149)
                     /gene="gltX"
                     /locus_tag="ACGE4U_000509"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFF8392698.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6319..7476)
                     /locus_tag="ACGE4U_000510"
     CDS             complement(6319..7476)
                     /locus_tag="ACGE4U_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFF8392699.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7510..8880)
                     /gene="radA"
                     /locus_tag="ACGE4U_000511"
     CDS             complement(7510..8880)
                     /gene="radA"
                     /locus_tag="ACGE4U_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFF8392700.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8943..9470)
                     /locus_tag="ACGE4U_000512"
     CDS             complement(8943..9470)
                     /locus_tag="ACGE4U_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFF8392701.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9700..10506
                     /gene="proB"
                     /locus_tag="ACGE4U_000513"
     CDS             9700..10506
                     /gene="proB"
                     /locus_tag="ACGE4U_000513"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFF8392702.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10503..11750
                     /locus_tag="ACGE4U_000514"
     CDS             10503..11750
                     /locus_tag="ACGE4U_000514"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFF8392703.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11826..12170
                     /locus_tag="ACGE4U_000515"
     CDS             11826..12170
                     /locus_tag="ACGE4U_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392704.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12287..12613)
                     /locus_tag="ACGE4U_000516"
     CDS             complement(12287..12613)
                     /locus_tag="ACGE4U_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392705.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(12986..14187)
                     /locus_tag="ACGE4U_000517"
                     /pseudo
     CDS             complement(12986..14187)
                     /locus_tag="ACGE4U_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14304..14819
                     /locus_tag="ACGE4U_000518"
     CDS             14304..14819
                     /locus_tag="ACGE4U_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8392706.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15018..15464)
                     /locus_tag="ACGE4U_000519"
     CDS             complement(15018..15464)
                     /locus_tag="ACGE4U_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFF8392707.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15511..16989)
                     /gene="msr(C)"
                     /locus_tag="ACGE4U_000520"
     CDS             complement(15511..16989)
                     /gene="msr(C)"
                     /locus_tag="ACGE4U_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFF8392708.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17343..17972)
                     /locus_tag="ACGE4U_000521"
     CDS             complement(17343..17972)
                     /locus_tag="ACGE4U_000521"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8392709.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(17956..18420)
                     /locus_tag="ACGE4U_000522"
     CDS             complement(17956..18420)
                     /locus_tag="ACGE4U_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8392710.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(18907..20175)
                     /locus_tag="ACGE4U_000523"
     CDS             complement(18907..20175)
                     /locus_tag="ACGE4U_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFF8392711.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20406..21143)
                     /locus_tag="ACGE4U_000524"
     CDS             complement(20406..21143)
                     /locus_tag="ACGE4U_000524"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8392712.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21210..21779)
                     /locus_tag="ACGE4U_000525"
     CDS             complement(21210..21779)
                     /locus_tag="ACGE4U_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFF8392713.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            21907..22779
                     /locus_tag="ACGE4U_000526"
     CDS             21907..22779
                     /locus_tag="ACGE4U_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8392714.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23121..23993
                     /locus_tag="ACGE4U_000527"
     CDS             23121..23993
                     /locus_tag="ACGE4U_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8392715.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            23990..25948
                     /gene="nagE"
                     /locus_tag="ACGE4U_000528"
     CDS             23990..25948
                     /gene="nagE"
                     /locus_tag="ACGE4U_000528"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFF8392716.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26199..27050)
                     /locus_tag="ACGE4U_000529"
     CDS             complement(26199..27050)
                     /locus_tag="ACGE4U_000529"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8392717.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27173..27640
                     /locus_tag="ACGE4U_000530"
     CDS             27173..27640
                     /locus_tag="ACGE4U_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFF8392718.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(27903..28385)
                     /locus_tag="ACGE4U_000531"
     CDS             complement(27903..28385)
                     /locus_tag="ACGE4U_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8392719.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28485..29348)
                     /locus_tag="ACGE4U_000532"
     CDS             complement(28485..29348)
                     /locus_tag="ACGE4U_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8392720.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         8450 a         6296 c         5390 g         9532 t
ORIGIN      
        1 taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct cattacttgc
       61 ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg catataataa
      121 tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat tgcttctttt
      181 aataaaagat gctgaatcgc taaaggttct tgcaatgtga tctctgctaa aaagtctggt
      241 ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg acgcttcagc
      301 gcgttctgct tcctcaacag agaatagatt tgattgctta taatgctttt ataataactt
      361 ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa agcgatcctt
      421 gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt tttcaaacgt
      481 agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc accattacgt
      541 gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt ttatggagga
      601 atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat tttttctcat
      661 taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg gagaattacg
      721 tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt ccgttttttt
      781 tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa agatcatcca
      841 ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat tcagctgacc
      901 agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa
      961 ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga agacaacgat
     1021 gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag cttctcttgc
     1081 atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac caatcatgtt
     1141 gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac gttgggaaaa
     1201 agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga cgtgcccggt
     1261 catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca ttcctttgcc
     1321 ttcattacca atgactaaag caattgatcc tttggcattc catttttgga agtttgtgcc
     1381 ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca cagcttgtac
     1441 caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag tctttgttac
     1501 gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga cgccacttgc
     1561 gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa
     1621 aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa gggataaata
     1681 ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc
     1741 taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga tttctttcag
     1801 ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg
     1861 ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta atgcttcttc
     1921 attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat atctattttg
     1981 ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt tacccatttt
     2041 tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg gtttgttttg
     2101 tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac gtcgtaacat
     2161 cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa taaagttctt
     2221 cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct tgtgcattcg
     2281 cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct ccttgatcaa
     2341 tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc aaggctaaac
     2401 cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt gcggtgtatc
     2461 ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc
     2521 acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt catctaataa
     2581 ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata aagcttctcc
     2641 ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca gccacttagc
     2701 aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat
     2761 gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg ccaaattctc
     2821 tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt
     2881 ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt attgtgtggt
     2941 cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat gtacagtgat
     3001 gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta catagccatt
     3061 gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg cgatttcatt
     3121 ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg tttctcctaa
     3181 atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt tgccccatgg
     3241 tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat ctaatgggtc
     3301 ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca gttcatcgat
     3361 ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat acacatcacc
     3421 tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta aaatatctgg
     3481 catatgatcc atgactcttg gatgcagtgt agcaggctcg acattcaacg ctttcgtatc
     3541 ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac ctaattcgtt
     3601 tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat cgactttata
     3661 gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac gagcatttcc
     3721 gatatggata taattataca cagtcggtcc gcagacatac ataccgacat gtcccttttg
     3781 tatagggatg aattcttctt tttttctggt caatgtattg tagattttta tcattctgat
     3841 ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac cgctgtagcg
     3901 tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt ttttccgatc
     3961 gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt aacagttggg
     4021 tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa taaaatcaca
     4081 tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc gatgaacacg
     4141 ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg ccagaattga
     4201 gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga gaaacgatgc
     4261 caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga acgtgcagcc
     4321 ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac tttccactcc
     4381 tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa
     4441 taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat aatcaatgat
     4501 tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct ataaaacagt
     4561 ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc aaccattcat
     4621 tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact tttttctgtt
     4681 catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg accaagcaat
     4741 tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac acggattggc
     4801 atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat ggcagctttg
     4861 acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc gtggagaaca
     4921 gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac aggatgttca
     4981 ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg aggttgatac
     5041 aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc tgctacacgt
     5101 ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc cattgctttg
     5161 atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg tgatttactt
     5221 aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc
     5281 acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag atagcctaat
     5341 tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag ttttttacct
     5401 gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct
     5461 tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg taaaacgtga
     5521 gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg cataccatcg
     5581 cgtttttgta tgacaaagtc gccaccgata ttatcggatt cgaacgaaat agcgcctttc
     5641 accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac aacagattct
     5701 agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg
     5761 taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct
     5821 gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag cggttgatag
     5881 atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc aggagattca
     5941 tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc atcttcgata
     6001 ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt gtggcgggca
     6061 aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc agttggactt
     6121 ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac tagactttga
     6181 ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta aagccactca
     6241 gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat ttccttacca
     6301 gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct cttttttatt
     6361 gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag cagctgtttg
     6421 aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc catcttctac
     6481 gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg tcccggcttt
     6541 tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg catttgccaa
     6601 cgcattgata ttaagaacag gtacattttg gaattcagag actttattca agttatagtc
     6661 attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc tgtctacttc
     6721 actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt ctttttgcaa
     6781 ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat cccctgagtc
     6841 cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc cttctaagaa
     6901 tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat ccaagatttt
     6961 atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa cttgactagc
     7021 tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt cttcgattcg
     7081 tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa tcggcaatac
     7141 actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa cacctaaagt
     7201 caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga cctcacttag
     7261 tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca aagataataa
     7321 aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc atccgtttga
     7381 ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc ttgcgccgac
     7441 aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc
     7501 atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta ctccaactat
     7561 ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt atacttttgt
     7621 gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac gtctgatttc
     7681 tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt tttctttata
     7741 gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta attttacccc
     7801 acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc ttttttccag
     7861 cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc gtttggcatt
     7921 tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac gtgtcccttc
     7981 cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta agaaaatctg
     8041 tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt
     8101 cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat gttgttcacc
     8161 ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg cgatcgagcc
     8221 ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag cgattttgag
     8281 tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg tgatgtctgg
     8341 ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat gttcgatggc
     8401 acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt tgatacttcc
     8461 gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata catataagac
     8521 ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg atttcccgat
     8581 acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc ctaacacacg
     8641 gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga cgtctgcgat
     8701 ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc
     8761 ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa
     8821 cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt tttttgccat
     8881 tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt atctttaacg
     8941 ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg atcttgatct
     9001 gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt gatcactaca
     9061 tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc ctcattattg
     9121 taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg cttcaacgga
     9181 ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca
     9241 gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt tttcaatcca
     9301 tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg aatcacgacc
     9361 tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg cggtagattg
     9421 atgttttgat ctttatattt agaaataact tcaaatcctc tttttctcac cggttttcct
     9481 ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa
     9541 ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac
     9601 tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata
     9661 gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat
     9721 gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc
     9781 aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag
     9841 gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca
     9901 gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg
     9961 atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg
    10021 acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag
    10081 cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa
    10141 ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt
    10201 gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg
    10261 actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg
    10321 gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa
    10381 gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg
    10441 gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg
    10501 aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac
    10561 ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga
    10621 atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata
    10681 tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta
    10741 cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga
    10801 cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca
    10861 tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga
    10921 atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat
    10981 tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg
    11041 atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga
    11101 ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca
    11161 ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg
    11221 cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa
    11281 cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg
    11341 tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg
    11401 ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag
    11461 tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag
    11521 aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg
    11581 cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg
    11641 cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat
    11701 taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt
    11761 gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg
    11821 tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg
    11881 cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga
    11941 gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac
    12001 gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt
    12061 ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat
    12121 cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg
    12181 tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg
    12241 acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg
    12301 gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat
    12361 aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt
    12421 cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt
    12481 ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca
    12541 accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat
    12601 attcttccag catctattat ccccccattc tttttcttaa atgaatttta gcttacctac
    12661 tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca
    12721 catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat
    12781 attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga
    12841 cagtctcaca acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca
    12901 accaaacgaa aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga
    12961 cagctttttc acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt
    13021 gataaatgat acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc
    13081 tttcaccaat atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa
    13141 agcggacaaa actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag
    13201 caatcgtcgt caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga
    13261 tcggtacaag gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata
    13321 agacaaatgc tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa
    13381 cgaaaatcga agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta
    13441 attcagaata gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat
    13501 tatataacaa tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac
    13561 cttcccaaag gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg
    13621 ctataaaacc gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac
    13681 tgcccaagac cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat
    13741 acatcatgat tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag
    13801 tagagacaaa taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga
    13861 tattcccaga taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca
    13921 atgttttttc aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt
    13981 gactaacatg taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc
    14041 agcaatcgtt tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt
    14101 agctgctaca actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat
    14161 atctatttga ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta
    14221 ttttacatgt aaatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa
    14281 ataagacgag aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt
    14341 tatccaaacc acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc
    14401 atcacagtca gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt
    14461 tggacaaaga cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc
    14521 ttgtagagga atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag
    14581 caaaaatcta tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta
    14641 gagaacaaca gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc
    14701 tagacaagaa acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc
    14761 agcaggaact ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag
    14821 ggataaacag aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag
    14881 caatacagtt ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc
    14941 actatttttc tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt
    15001 caaataacag tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc
    15061 gggccaacga tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc
    15121 atataagcag agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac
    15181 atacatgtca ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg
    15241 ctttctggat ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag
    15301 acatccaaaa tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa
    15361 tgaagatctt gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac
    15421 atcattgttg taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt
    15481 aaatttgtca cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat
    15541 tttctgccct tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg
    15601 agaagtaaac aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt
    15661 tgccatatca ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa
    15721 aagcaaagca atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt
    15781 caaagtttcc tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa
    15841 ctctgtttgt ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt
    15901 ttgctgatat acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg
    15961 gatatgttct aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt
    16021 acctaaagaa aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg
    16081 gacattttgt cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa
    16141 acggatttgt ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc
    16201 taatgttttc gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga
    16261 caaccgatcc ggtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc
    16321 cgcttgtttt ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga
    16381 ctctgcttct cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata
    16441 attccccgaa aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc
    16501 atctagaaaa tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc
    16561 ttcaatcagt cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat
    16621 cccttgttca taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct
    16681 ccatttccgc tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt
    16741 taggtccttt gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt
    16801 ttggattttc ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt
    16861 accgacacca ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc
    16921 aatagatagt tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa
    16981 attttccata tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa
    17041 gagcagaaaa atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat
    17101 aactatgact aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa
    17161 aatggacata ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt
    17221 gttattcaaa agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt
    17281 tattaggtta ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc
    17341 ttttattcga acgtagtgat cttttctggt gctaaacgaa cagactcgta accttcttct
    17401 accgcttttc ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat
    17461 gcctctgcca actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca
    17521 cgcgcaacta acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc
    17581 atttcttctc ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg
    17641 acttcttttg gcatcttccc tgattcgtaa gcttggttat agatttcttc gccatattcg
    17701 taacattgca tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta
    17761 ttgaaacgga tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag
    17821 cgccatggtt gcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc
    17881 atttcttcgt gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc
    17941 ataattgttg aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt
    18001 tatattgcaa agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac
    18061 atcgctgaaa gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctc
    18121 gattttcttc tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt
    18181 tctctgacgc gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc
    18241 ttgatcaatc tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag
    18301 tctcatcatg atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac
    18361 gattttttga tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat
    18421 aatttcctcc tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa
    18481 aaagaaaaaa gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat
    18541 agagaaaaga aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa
    18601 aaccaagatt attgattctt atcaaactat agaagatagc tcttctgcag tagaaaattt
    18661 tacaccgcca aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt
    18721 ggatcagtat gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt
    18781 tgagtaaacg gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca
    18841 tgaaaatctg tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac
    18901 ctctttttaa taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca
    18961 atctctttcc ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa
    19021 tagtcgataa attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc
    19081 gatttttgtt aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta
    19141 aggattgtat aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc
    19201 atattcccag cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg
    19261 ttctcctgta tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc
    19321 atactgtgtc agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc
    19381 agtggcataa cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt
    19441 ccatgcaccg gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac
    19501 gttatcttgc aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat
    19561 ccattgccca ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc
    19621 tttaatccca aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact
    19681 ttcaacgatg gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact
    19741 ttgtgcagtt gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc
    19801 ataaaaagca tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt
    19861 caatgtttgc ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg
    19921 agcttctgct gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc
    19981 ttcggttgaa attgtatttt ctgagacagt agctgactgc aaagtactct ctgtatcgtt
    20041 ttcaattcct gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc
    20101 agctagcact gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa
    20161 attctttgct ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt
    20221 aactaaaaaa gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttgat
    20281 aaatttgctt tttctacata aaaagacgta aatgaagaat attcttctcc attcacgcct
    20341 taaattgatg gtcgtttgaa taactattta acaagtaatt ctctttggat attatccgtt
    20401 atggattata cttcctgtgt cgtcggacgg ataatcatgt tactgacaga catccggtct
    20461 ggcgtttcta tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa tccccattct
    20521 ttttgcgctt catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca
    20581 gtttgaacag ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga
    20641 cgtaatcctt ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca
    20701 ggataaacaa catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt
    20761 tccatgattg gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc
    20821 atattttgcc attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca
    20881 ttattgtaca atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg
    20941 acttgtgcaa aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat
    21001 tcttttattg cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt
    21061 tctgctaata gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc
    21121 actttttctg ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat
    21181 actcactatc tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt
    21241 ttggcaggga ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc
    21301 gctgcaacaa tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg
    21361 atccaagcat ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga
    21421 tcggttagat ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct
    21481 aaacagatgc cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct
    21541 atactaatat gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct
    21601 actttgtcac taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca
    21661 gtattcagcg ctgctaaagg ctcttcgttt tcagcttgga tctgatggat cacttcaaag
    21721 agcggatccg ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt
    21781 ttgctccctc cttttatcct tactttataa cggacggctt atcatgtcta atacttataa
    21841 aacatactta actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg
    21901 gaaaaaatgg atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc
    21961 agccgagcag caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat
    22021 ttagaagaag aacttggtgt gacacttttc actcggggct cccgagagat acagttgacg
    22081 gattctggtc gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg
    22141 gcgaacaatt taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa
    22201 actaaagcaa tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat
    22261 atccagtttc atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga
    22321 ttacttgatt ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga
    22381 ctgccagccc aagatcgttg gggacttttg atacatcgaa atcatccttt agcaaagcaa
    22441 accgtcatcc atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca
    22501 gtggacagtc aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga
    22561 acatataact tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta
    22621 gctctagatg ggatcatcaa tacttatggg acggaaaaaa tattcatccc actcgatccg
    22681 ccattacaag cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct
    22741 cgtaaatttt tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct
    22801 ttcagctttt taacaacgaa taagataagc aaaagatcca acgattaaat aacataagtt
    22861 tcttaattta tttcttcctt ttatttctga taattccagt aaattactat aattataaaa
    22921 aaagaattga caaaacataa ctgtctatat aaaattgatt acgttaacaa taattcgttt
    22981 catgtgacta gatcatacta ggcatgaaaa atctctgagg aacatggttt ccctaggagg
    23041 tttttcgtgt ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa
    23101 tcttcaaaaa gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat gcagtattgg
    23161 tagatgacgg tggacaggaa aaagtagcta ttggaaaagg gattggattt gacagaaaaa
    23221 gaaatgattt gatttttgat cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc
    23281 aaatcaagtt gcaaaattta cttaatcaga ttgatgaacg ctttttattt gcagcagaaa
    23341 cgatcattga tcatgcggaa actgttttga tggaaaagtt gaatgaacat gtattgatcg
    23401 ctttgaccga ccacattgct ttttctgcgg agaacattaa taacggtatc gtcatccgca
    23461 acaaactttt aagagaaatc gaagtgcttt acagtgatga attcagtatt gctcaatggg
    23521 cagtagagta tctgacaaaa gagcttggga ttccatatac ttatgacgaa gcaggatata
    23581 tcgcaatcca tattcacagc ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg
    23641 aagtgacgat tgtatctgac atcatccatt tagtggaatc agaattggaa acagatatcc
    23701 attcagaacc attttcactg aattattcaa gactggtgaa tcatttacga ctgcttctac
    23761 aacgaaccca tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa atggtcaaac
    23821 gaaaataccc agaaagctat aagatttcta aaaaaatccg tgtgttgctg accaaagaat
    23881 accagctcgc gatcacgaaa gaagaactgg gttacttggc catccatatc gagcgtttaa
    23941 gaagcgcaat cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat tgaaagcata
    24001 tctacaacga atgggacgtt cccttcaact tccagttgcg gtcttacctg ctgctgcttt
    24061 actagtaggg atcggaaact ggtgggcatc ttatagtaac gacattgtcg ctcatttttt
    24121 acaagctggc ggtaacgcag ttttaggtca attacctttg ctgtttgccg ttgggttagc
    24181 actagggatg tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga
    24241 agttccagta aatgttttaa aaactgattc agtagctact ttattgaata tcaaaccaga
    24301 atcagttgat ccatcttttg ctcagatcag taacgtattt attggtatta ttgccggatt
    24361 gattgcggca gctctttata atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt
    24421 ctttagcggc aagcgtctag tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc
    24481 agtgctactg ctcgtatggc ctccggttta ctctgcttta gtttcttttg ctaaattcat
    24541 tttgagttta ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc
    24601 tacaggattg catcaagcaa tgaactccgt gttctggttt gacgtagcag gaatcaatga
    24661 tattggtaaa tttttggctt ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg
    24721 atttttccca atcatgatgt tcgggcttcc agcaggagca tatgctattt acagaaatgc
    24781 acgtccagaa aacaaagcaa aaacagcttc cttgatgcta gcagcagctt ttgcttcatt
    24841 ctttactggt gtgacagaac cacttgaatt ttcatttatg ttcgttgctt ggccattata
    24901 tgtcttgcat gcaattttca ctgggatttc tttagcagtg agtgcattct tccattggac
    24961 agcaggattt gcctttagtg ccggatttgt cgattacttc ctatctttga aaaatccgat
    25021 tgcgaatcag ccattgatgt tgatcgtcca aggattagta acagcagtga tttactactt
    25081 tggctttaac tttgctatca aaaaattcca tctgatgact cctggccgcg aggaagcaga
    25141 tctttcagat gacgatactg caacgactaa ttcagacaat aaatatgcgg cacaagcaag
    25201 caaaatctac gctgcattag gcggagccga caatgtaact tcgatcgata actgtactac
    25261 acgccttcgt ttgcaagtca aagatacagg cctgatcgat caaaataaaa tcaaagccac
    25321 tggcgtacct ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga
    25381 agtacaattc gttgcagatg agatggcaaa actacatggt ggtgctgcag cacgacctgc
    25441 acaaacaaac accgtggtta aaacagaaac atttgccggt gaaacgatcg aacaagatat
    25501 ttacgctatc gcaaatggga aactgattcc aatcactgaa gtttcagatg atgtattcgc
    25561 ggaaaaaatg atgggtgacg gctatgctgt attaccagaa aatggagaaa tcttctctcc
    25621 aatcgctggt acgatcacaa acattttccc aacgaaacat gctgtcggaa tccaaacaga
    25681 tgctggtatc gaagtcttgc ttcatatggg catcaacact gttgacttaa aaggcgaacc
    25741 attcacttta tatgtagaag aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt
    25801 tgatcttgcc gctattcaat ctgcaggcaa aaacacggat atggtcgttg tattcacaaa
    25861 tggcgacaaa gtccaaagct tagaaatcga acccgcacgt gatgtaaaag caaatgataa
    25921 aatcggctct gtttccaaca aagcctgagc catcgtcttc cccagacttt tttatcgata
    25981 aacaaacagg agctgagaca aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg
    26041 ttctcctgcc aactccgcgg taataagtcc agctaaacga aacatatgaa gagctatttt
    26101 ttatttgact cttcttatta cttgatgcag aacagctttt tcacaaaatt tttcttcata
    26161 ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct
    26221 tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag
    26281 gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct
    26341 tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata
    26401 atttctaact ctttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt
    26461 agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca
    26521 gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc agcataaacg
    26581 aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta
    26641 ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga
    26701 tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa
    26761 gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct
    26821 ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct
    26881 aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt
    26941 tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc
    27001 agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact
    27061 ccctattctt tttatttgtt aagattcaca gctgtacatt tataaattac acctttatca
    27121 taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta
    27181 ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac
    27241 ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg
    27301 taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca
    27361 gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga
    27421 aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc
    27481 gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga
    27541 aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa
    27601 aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca
    27661 tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt
    27721 catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata
    27781 aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc
    27841 tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat
    27901 tatcatggtt tattgactac cggtatccag atttctccca tatatttccc gttggtaact
    27961 cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa
    28021 acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa
    28081 gcgcttacaa caagatacgt tccttttggg aatgggatga ttcgcgtagt gtttggaaca
    28141 tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact
    28201 gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata
    28261 actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc
    28321 ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt
    28381 gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat
    28441 gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc
    28501 actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag
    28561 atctcgggag cttcgactaa cccataatct gaagaaggaa gccattctgc atagattttc
    28621 ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg
    28681 tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag
    28741 ccaatcatgt gcatcaaact gcccttttct tccaagcggt ctccatcaaa tccataagaa
    28801 acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc
    28861 tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga
    28921 acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc
    28981 gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg
    29041 tatctactga tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct
    29101 tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc atctagaaga
    29161 tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca
    29221 ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt
    29281 ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct
    29341 ttaagcatat ttatcttcct ccttgattgc ctttttatca tattatcaaa gaaagtcaat
    29401 tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga
    29461 aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac
    29521 ctttagaaaa ataaattttg gggaagacag attctaagta aacaaagcgg taaaaatatc
    29581 ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac
    29641 tgctgatcag aacgctgctt ggattttt
//
LOCUS       DAXWLF010000016        28196 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXWLF010000016 DAXWLF010000000
VERSION     DAXWLF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.016"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            117..1952
                     /gene="tdc"
                     /locus_tag="ACGE4U_000533"
     CDS             117..1952
                     /gene="tdc"
                     /locus_tag="ACGE4U_000533"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFF8392721.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2257..3735
                     /locus_tag="ACGE4U_000534"
     CDS             2257..3735
                     /locus_tag="ACGE4U_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8392722.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3701..5032
                     /locus_tag="ACGE4U_000535"
     CDS             3701..5032
                     /locus_tag="ACGE4U_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8392723.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5146..7698
                     /locus_tag="ACGE4U_000536"
     CDS             5146..7698
                     /locus_tag="ACGE4U_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFF8392724.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7705..9144)
                     /locus_tag="ACGE4U_000537"
     CDS             complement(7705..9144)
                     /locus_tag="ACGE4U_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8392725.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9119..9301
                     /locus_tag="ACGE4U_000538"
     CDS             9119..9301
                     /locus_tag="ACGE4U_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392726.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9490..9852
                     /locus_tag="ACGE4U_000539"
     CDS             9490..9852
                     /locus_tag="ACGE4U_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFF8392727.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10039..10896)
                     /locus_tag="ACGE4U_000540"
     CDS             complement(10039..10896)
                     /locus_tag="ACGE4U_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8392728.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11016..11927
                     /locus_tag="ACGE4U_000541"
     CDS             11016..11927
                     /locus_tag="ACGE4U_000541"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFF8392729.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11934..13580
                     /gene="mdcA"
                     /locus_tag="ACGE4U_000542"
     CDS             11934..13580
                     /gene="mdcA"
                     /locus_tag="ACGE4U_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFF8392730.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13682..13981
                     /locus_tag="ACGE4U_000543"
     CDS             13682..13981
                     /locus_tag="ACGE4U_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFF8392731.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            13971..15647
                     /gene="mdcD"
                     /locus_tag="ACGE4U_000544"
     CDS             13971..15647
                     /gene="mdcD"
                     /locus_tag="ACGE4U_000544"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFF8392732.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15651..16244
                     /locus_tag="ACGE4U_000545"
     CDS             15651..16244
                     /locus_tag="ACGE4U_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFF8392733.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16268..17344
                     /locus_tag="ACGE4U_000546"
     CDS             16268..17344
                     /locus_tag="ACGE4U_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFF8392734.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17369..18238
                     /gene="mdcB"
                     /locus_tag="ACGE4U_000547"
     CDS             17369..18238
                     /gene="mdcB"
                     /locus_tag="ACGE4U_000547"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFF8392735.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18349..19113
                     /locus_tag="ACGE4U_000548"
     CDS             18349..19113
                     /locus_tag="ACGE4U_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFF8392736.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19602..21695
                     /locus_tag="ACGE4U_000549"
     CDS             19602..21695
                     /locus_tag="ACGE4U_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8392737.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21720..22022
                     /locus_tag="ACGE4U_000550"
     CDS             21720..22022
                     /locus_tag="ACGE4U_000550"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8392738.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            22049..23338
                     /locus_tag="ACGE4U_000551"
     CDS             22049..23338
                     /locus_tag="ACGE4U_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8392739.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            23357..23764
                     /locus_tag="ACGE4U_000552"
     CDS             23357..23764
                     /locus_tag="ACGE4U_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8392740.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            23787..24656
                     /locus_tag="ACGE4U_000553"
     CDS             23787..24656
                     /locus_tag="ACGE4U_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFF8392741.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            24658..25278
                     /locus_tag="ACGE4U_000554"
     CDS             24658..25278
                     /locus_tag="ACGE4U_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8392742.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            25728..26333
                     /locus_tag="ACGE4U_000555"
     CDS             25728..26333
                     /locus_tag="ACGE4U_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFF8392743.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            26468..26920
                     /locus_tag="ACGE4U_000556"
     CDS             26468..26920
                     /locus_tag="ACGE4U_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8392744.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            26957..27454
                     /locus_tag="ACGE4U_000557"
     CDS             26957..27454
                     /locus_tag="ACGE4U_000557"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8392745.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            27472..28182
                     /locus_tag="ACGE4U_000558"
     CDS             27472..28182
                     /locus_tag="ACGE4U_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFF8392746.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
BASE COUNT         9273 a         4828 c         6010 g         8085 t
ORIGIN      
        1 gtggatttga tgttataaaa aaaaaattct catttttggc gctatagtga agaggtgtta
       61 cagataaaca catgtatctg caagacattg accaacaaaa tatggaggga agaaaaatga
      121 aagatatgga tatcaaggcc gtctttatag gcgacaaagc tgaaaatgga ccagtttata
      181 aaatgttgtt gaacaagatg gtagatgaac atcttggatg gagagaaaat tatattcctt
      241 cagatatgcc tgcaattagc gaaggagata agctcactcc agattatcta gccacccgcg
      301 accatatgat agaggtcttg gatgaagtca gtcagcgatt acgcgcagga tcgatcccat
      361 ggcattcagc aggccgttac tggggacaaa tgaatgctga aacattgatg cctgctttat
      421 tagcttacaa ctatgcgatg ctgtggaacc caaataatgt tgcattggaa tcttctatgg
      481 caacttcgca aatggaagca gaagtaggac aggattttgc ttcattattc aatatgacag
      541 acggatgggg acacattgca gcagatggtt ctattgctaa tcttgaagga ttatggtacg
      601 cccgttgcat caaatcgatt cctttagctg tcaaggaagt gctgcctgaa aaagtgaaaa
      661 aaatgtctga gtgggaactt ttgaatttat ctgttgaaga aattttggaa atgaccgaaa
      721 gctttacgga cgaagaaatg gatgaggtca aagcagcttc ttcccgtagc ggaaaaaata
      781 tccaaagatt agggaaatgg ctggtccctc aaacaaaaca ctattcttgg atgaaggcgt
      841 tagatatttg cggagtcggc ttagatcaga tggttgctat cccagttcaa gaggattatc
      901 gaatggatat taatgctttg gaaaaaacaa ttcgtgaatt agctggacaa aaaatcccaa
      961 ttctaggtgt ggttgctgtc gtaggaacga cagaagaagg tcaagtagac agtgtagata
     1021 aaatcgtcca attaagagaa aggttaaaag atgaagggat ctatttctat ttacatgttg
     1081 atgccgcata tggcggctat gcgcgttcac tgtttctaaa cgaagcagga gaatttgtgc
     1141 cgtatgcttc cttagctgaa ttctttgaag aacatcatgt tttccaccac tgcgtaacga
     1201 ttgacaaaga ggtatatgaa gggttcagag ctatttcaga agcggattct gtcacgatag
     1261 atcctcataa gatgggctat gtcccttatg ctgctggagg aatcgtaatc aaacataaaa
     1321 atatgagaaa tatcatttct tatttcgcgc catatgtgtt tgaaaaatca gtaaaggctc
     1381 cagatatgtt aggagcttat attttggaag gatcaaaagc tggagcaacc gccgccgcag
     1441 tctggacagc acatcgtgta ttgccattga atgtgaccgg atatggtcaa ttgattgggg
     1501 cttcaatcga ggcagctcaa agattcagag aatttctcga tcatctgact tttactgtta
     1561 aaggaaaaac aatcgaagtc tatccattga atcatcctga ttttaatatg gtcaattggg
     1621 tattcaaaga acaaggctgt acagatttga acgctatcaa tgaattaaat gaaaaaatgt
     1681 tcgatcggtc ttcttatatg gatggcgatg tttatggtga acggtttatt acttctcata
     1741 ctacatttac acaagaagat tacggtgatt ctccgattcg ttttgttgaa agaatgggtc
     1801 taacgaaaga agaatggaaa aaagaacaga aaatcactct gctacgtgcg gctatcatga
     1861 ctccttattt gaatgatgat cgaattttca atttttatac aaaaaaaatc gccaaagcaa
     1921 tggaaaagaa actaaacgaa atcatccaat agaactgatt tttgtgtgaa agaataacaa
     1981 gagacatatg gagaacagac aattatttgc ttcatcaaaa caattgtatt tccccctgtt
     2041 tttcatatat tcctcgccaa gatattcttt tttataaaac gttctataag ttggatcgtt
     2101 cgtttcatgt atcaaaccca gttcactaag atcgttcaat ggatgtttcg gtgatactgg
     2161 gttttgtact gaatcgatga aaccttgtca tttctgttct tgaacaaaaa taaaagaaaa
     2221 caaaaacata tgtcgctatt gaaagaagga ataaagatga aagatactgg ttcatcaaag
     2281 caaatcacga ccatgacctt tatcggaatg acctgcgccc ttgtagcaag tgtacgaaat
     2341 attccagatg tagcagcaac gagctggacg atgatttttt acatgctggt tgcagtatta
     2401 ttgtacgctt ttccaatctc attgatctct ggtgaatttg ctggaatgtt tccccaaaaa
     2461 ggtggtccag aactttgggt atcgaatgct ctaggaaaaa aatgggggtt cgttgtctca
     2521 tggctgttat gggtccaaat gtttcctggg atggtgatgg tcgcttcagc actcgctccg
     2581 ttgtttggta atattatcga taatgtgccg cttggtttga acagcaaatt tacattagtt
     2641 gtcattttag tcgtttattg gatcatcact tttttgaatt tgaaatttga tatggcaaaa
     2701 atcggcggaa aagtcggtgt ttggttagga ttgtacgtgc ctttgacgat gatgctctta
     2761 ttagggtttg ctgcgtggat caaagttggg atcgtaccaa ccggaacatt aggcagtttt
     2821 tcatgggaca aattaatacc agatactacg actgcatcct catttgttta ttttgcgccg
     2881 atcatgttta tttttactgg tatcgaaatg tcctctgtgt atatcactcg attggaaaat
     2941 ccagttaaaa cgtatattcg cggagtattt gccgcgttaa tttttatatt tttcgtcaat
     3001 attttgaatg ctttagttgt agcaaatgtc gtaccaaaag gacaaatgga attgaataat
     3061 atcgcgcaat ctatttcgat ttattgccaa attcttggtt tgccacatct tattgtgaat
     3121 ctgtttagtt tacttgtctt cattggtgta gcagtccaat tatctgcttg ggcctcagga
     3181 ccagcaaaga cagtgacaga aagtgctaga aaaggtgcgt atccaccaaa attcaatttt
     3241 tggaagacga atcaatttga cgtatccaaa tccgttattt tgactcagtc tgtcattatc
     3301 tcaatctttg cactttttta tttgctgatt cctggcgtca atcaagcatt tttgatgctc
     3361 gtaaattcaa ctactgttat ctactgtatc gtctatgtca tcatggggat cgcagttttg
     3421 agattgcgtt atacacatgc aaagttgaat cgaccattta gaattggaaa aaaagagaaa
     3481 aagagcaatc ttggagtttg gactgtcgtt attgttttat ttgcggcaat cggattttct
     3541 gtcggactga cgatgaaagc tggaacatgg atcaatctaa ttgcagtcac agccatcagt
     3601 gtcattttgt ttgtagtacc actctgtatt gaaaaaataa agaaaccttc ttgggaacag
     3661 gaagtcgcca tgctgcttga aaaagaaaaa ggtgaacttg atggaaaaac cggcaatgaa
     3721 acggactcta actaatcgaa atatccagtt aatcgcatta ggtggcgcaa ttggaacagg
     3781 attattttta ggttcagcaa aggcaattgc atcagcaggg ccagctgtta ttctcgttta
     3841 tttgatatgc ggtttggcta tttatggttt gatgcgggcc atgggtgatt tatttttagc
     3901 aaatagtaca tttaactcta tcagtgattt cgttgactat tatttaggca aaaagtgggc
     3961 attttttgtc aattggactt attggctttg ctggcttgga gctggacttg cggaattaac
     4021 agcaatagga ttgtatatcc atttctggtt tgtacatgta ccagctttga taagcggagg
     4081 tagcgcacta atgatcttaa tggcagtcaa ccttgttgca gtcaagtggt ttggtgaatt
     4141 ggaatctttt ttctcaatca tcaagatctt ggggattttc ctttttatct tattgggtat
     4201 aggattgagt gtctcagatt ttcataccca gtctatcgga atcaaagaaa attttggaca
     4261 gatcgatgaa ttactaccgt ttggctttgc tggaatggta gtctcatttc ctatggtttt
     4321 attcagtttt gctggaattg aaatgatcgg cttaaccgtt ggcgagacaa gtgatccgca
     4381 aaagaatttg aaaaaagcaa tcaactgtct gccttggcgt attctctttt tctatatagg
     4441 aacgattttg tcgattttat ttgttgttcc atggcattgt ttagatttaa aagaaagtcc
     4501 tttagtgact atgctgcaag tgatccattg ccaaggcgga gcagcaattg tcaatctaat
     4561 gattttggtt gcttctttgt cctcagcaaa tagtgcagtc tacagtacta gcaggatttt
     4621 atatcaaaca gcaaaagaaa aaagtttgcc ccattggttt tgtgcgcttt ctaagaaagg
     4681 cgtaccttta cacggtattt taattacagg aatgttattt tttgccggtc tttgtctaca
     4741 tttttttgta gctgatagtc gattgttttt tcagcttttg gcaggcatga caacgagttg
     4801 ttttctattt gtgtggtctt cgatcttgtg tgcacatgtt caatttatta aagaaaagaa
     4861 acggaacact acacaaagat ataaagatct tgctttgttg gtgtttttta cttcaattgc
     4921 tttcagcttg ttattcgaaa gcacgacacg ctttattcct tttttattgg ttatctggtt
     4981 tgtattctta aattttgtat ataaaagaat aagcaataga aatatcagtt aggcccagat
     5041 acattttgca aaacgaatta tttcagaccc ccctgaaata atcgatacgc ctgaaaaaac
     5101 ttccctgcgt ataataacag tatcaattaa acaggagggg ataggatgaa ctggtacaga
     5161 ttgacggtag aagatgtatt gaaaaactat caaacatcaa cagaaggctt gtcgcaagaa
     5221 gaacgacaga aacgcctgac tgatcaagga tataatgaaa taaaagcaag gcagcagaca
     5281 aaacgatgga aaaaaatagc aaagcatttt actgatctgc tgatgatcgt gttgatcatt
     5341 gcctcttttt tgaaatttgc ttctagtgaa tatattgaag gaagtattat tttattcgta
     5401 gtgatcgtca atggactcgt tagttactgg caagaaagaa aagcggaaga atcattgaat
     5461 ggtttgaagc agctgatggg acaagaagcg atagtactat caaacgggat gcaagaaaaa
     5521 atccctgcga aagaactggt aaaaggagat gtcattgtct tacgtgccgg agatgtcgtt
     5581 ccagcagata tccgattaat cgaagcacat gatttattag tggaagaatc gattttgacg
     5641 ggagaatcag aagcatctga aaaaagtcat gccgttttga cagaagaaga atcgatcggc
     5701 gaccagaaaa atatgggttt ttcaggaacg cttgtacaat ctggttctgc agtcggtgtc
     5761 gttgtagaaa caggaaacac gacagaaatc ggtaaaatca atcaagcatt gcaatcggtc
     5821 gaacaacaaa cgacgcctct gattaaaaaa atcaaccgat taaacaaaca aatttttcaa
     5881 ggcattcttt gtttgagcct attcttagtt atttttacga catttcgata tggtatggat
     5941 tggcatattc ttctttcggc tacgattgct ttggtggtgt caatggtacc cgaaggattg
     6001 ccagctgttt tgaccatgat tctatccgtt ggagtacatg aaatggcaaa agaaaaggcg
     6061 atcatcaaag gacttccttc ggtagaaaca ttaggttcta tgacagtgat ctgttcagat
     6121 aagacaggaa cattgacaaa aaatgaaatg acagttgtgg atgtagctgc aaaagaagaa
     6181 gcctgcgtgt tgtccattat gaaaaactgt caggaattaa aaacaaaaga agaacagaaa
     6241 acagaaaatt taagtggcaa tccgacagaa gttgcgctga ttcgacacac agaaacagcc
     6301 aatctacctt tgaaaaaaat agaagcaaag attccgttca gttctgaata caaatatatg
     6361 gcaacgatgc atgcggagga aaaaggagcg gtgatttacg tcaaaggtgc accagaagta
     6421 ttatttgcta aatcaactct ttcttctgca gaacaagaag catggagtca gacagctgca
     6481 gaatttgcga gaaaaggtca gcgtgtttta ggatttgctt ataaaaaagt agattcgaaa
     6541 caggaactca cgcatgagac tttaacccaa ttaacttttg cagggatcgc cggattgatc
     6601 gatccgccaa aagaaagtgc agtcaaagct gtaaaggaat gccaacaagc agggatctca
     6661 gtgaaaatga ttacagggga tcacaaagac acagcaaaag cgattgcgga acaaattggc
     6721 ttaaaacata ctgccaaagt tttagaaggg atcgatttgg atcttatgtc tgatgaagag
     6781 ttgattcagc aagtgccaat agtcgatgtc tttgctcgaa cgacaccaga acataaactg
     6841 cgaattgtta aagctttgca gaaaaatgga gaaatcgtcg gaatgaccgg agacggcgta
     6901 aatgatgcac ctgctttgaa aagatccgat gtaggaattg ctatgggaat caaaggtagt
     6961 gaagtaagca aacaggcagc tgatatggtt ttaggagatg ataatttcca tacgattgca
     7021 aaagccgtaa aagagggccg gcggattttg gataatttgc aaaaaaccat caatttcttc
     7081 ttgccaactg cattagctca aggattgatc ttgatttggg cacttatgct gaatcgcccg
     7141 ctacctttgt ccccagtaca gattttatgg gtcaatatgg taacgacaat cactttatct
     7201 tatgcactag ggttcgagtc gccagatcca gaaatcatga agcgcccacc aagagatcca
     7261 aagcaaggaa tcctatctgg ttatcatttg tttcggatca tctatgtctc cctactgatc
     7321 atggttcctg cctatttatt ggctatgcag tttgaagggc aaagcttaca gcaaaccatt
     7381 cttctgcaaa atatcgtctt agctcaagct gtttacatga tcaattgcag agagttgtcc
     7441 aagccgtcat tgaaccaagg tcttttgcaa aacaaagcgc tttttctttc attaggtatc
     7501 cttgctttat tgcaagggat ggttattttt ctaccagtcg gccagcagct gatcggtaca
     7561 gtaagtttaa cgatgacaca acagttattg attggagcaa atgtgattct tctattctta
     7621 gttgtagaaa tagaaaaagg gatcatgaac aaactgtttc gcaaaaaaga aaaaacagct
     7681 agagaataca gtcgctagcc gttactataa aaaagttttg aaagtgagtt cagtcaaacg
     7741 attgagctca tttttcgttg gaatagaaga aatgtataca atagggatat ctagagaatc
     7801 tagagggaaa ttggaaatca agatatctgt tttttcaaaa gaaatagtgg aaagatcggt
     7861 tagttcgata ggtaagaagc agacacgttt tcctaagtaa tcttttaatt cctgctgcaa
     7921 gaacgccttc catgaaggtt ctccttggaa aataaaatag acattgaaca aattcggttt
     7981 gatagaaaaa atcgtttgtt ggatcagtag agacaagagt tggagaaagt aatttggttc
     8041 attgatttct atttcttttt ttagatcgtt cagtaaattc tctttctgtt gatacaagtg
     8101 tgcatattgc tgatcatatg tttctaaaaa acgatggtat tgacggtcca atgcttcaat
     8161 caatatcggc gattcatgat attttaatat gaggagagcc aagttatcag tcagagatgt
     8221 tttttttagt tgatcaagag acagctctga agctagttcc tctacaaacg tttcgaggac
     8281 ctctaaaaat ggagaatgat gcaggcagcg agcggtttct tgtccatagt tattgttgta
     8341 attccaactt tccagaaagc agaaagaagc aaaagaaagc tcagtgtctt taagataaat
     8401 accttccgtt aaatacaatt ttttcaatgc agagtcatac agggaggaga gtgtgctgtt
     8461 ggtatggata gaagaatcta atcggcaatt cgcttttata cggatcgtat tgataaagaa
     8521 ccaacatgtt ccgaaaatct cttctgtttc aaccgctagt tcagtttgac tgaggtttct
     8581 taaaaattga aaataatggg attcatggat cgaataatcc tcgaaaaaat agttgttatg
     8641 agtgaaagga atcagcaatc gatgataaaa agtacgaatc gcacgctctt ttccaagaat
     8701 acaagctgta tatttcgtca aatcgatctg caagtcgtaa cttgctaaac gctgattgat
     8761 tttagtcaca tgtcttttca atgtcccata agaaagccca tggtcggcta gaaaatcagg
     8821 gacagcaact ttttttgtaa aaagcagatt tttcagcagt tcgacactga ctgattgttt
     8881 gaaaaaaatc tcccaaaggt catttgttaa taaattttgc tggttttgca aactgattcc
     8941 tgcttgatcg ctgatgatgt cccagccttc gggcagctga gagcgaatag attgaaggtc
     9001 agaaaaaatc gttcgttcgg ttgtccctat gatttcagct aattcattta cggttatctt
     9061 tgtatgattc agcaattgtt ctactaaaga aacatgacgt cgaatatctt tttcggtaat
     9121 gatttttttc attagtgaat acatcaatca gactcccttc tattctaaga atacggagaa
     9181 aataaacggt ggtcaagttt caactgattt ttcaatcatt gtttattttt ctggagcatt
     9241 tactgatgat aagaatttca tcatgaactt gaaaaatgat accactaaaa tcaaaaaata
     9301 ggtgggcaaa caataataaa cattttaata gtagatggta gcaagaccaa cacaggcgga
     9361 ttgaacattt aagagttgat aaaaaagaaa aatatcgaag tatttgatta gcaactactt
     9421 gtattcattt ttctattctt acgtggtatc tttgaataaa gcgaatcaat aagaaagaga
     9481 tggaagcaga tgctaacgaa aaaagaacta atcgatcaca tcaatgaaac atacaatttg
     9541 tctccagaat atatttttaa aaaatttcct gattattgcg tattcagaca tcaaaaaagc
     9601 aaaaaatggt ttggggtcct gatgtctgtt ccagaagaaa aaatctatga tgatgcaagt
     9661 gaccatagga tcatcaatat tcttgatttg aaggtcgatc ctgaacttgg cggaattttg
     9721 cgaggaaaag aggggtattt tccagcctat cacatgaata aagaacattg gatctctatt
     9781 gatctacgta aaatagaaaa gctagatgaa ctagcagatt tgattgatgg aagttttaaa
     9841 tcaacgcaat gaatgtctag gacaatagag aagaggttgt gacagaaatg tttagcttca
     9901 agaaataaga aggaatttac gaaaattgct tctcaaattt ttgggaaatt tcggcttatt
     9961 ttctaagaaa ttacttctgg aacaccgttt gttcgaaaaa agagctgcga caagaatttt
    10021 gtcccagctc tttttttttt attttcctaa ataaaattcc cgcatcgttt ttgcaatcat
    10081 tttgatcaaa ggatgatctt ctgtgaagct agaagataaa taaattgttc gtgtcaaggg
    10141 caaatctttg atttcccgca agacgaaagg atcattttta tcgtaagacc aagaaacttt
    10201 tgggaaaaag ctaacagcta atccttgctt gacaatagaa cgtaatgtcg caggatcatc
    10261 tgtcgtcgaa ctgatcgtca attcgatact tttcgcttca agtgctgcat ccagtgtatg
    10321 acggaaggcg ttgctctttg acagcatcaa gatgggggtg cgttggatat cttctaattg
    10381 cagatgttct tttttagcaa gaggatgttt cttaggaatg gcgacataaa caggttcttt
    10441 catgatagga atattggtca tgccaggctt catctccgaa gtgatgtaca gtacaggatc
    10501 ggtaatatca tctggtaaat gatgttgcat gatcgttaat tggatatcag gatgcttttc
    10561 cataaaaatt cggaccaagt tcggaataga aacagaggct acctctaaat acaatatgac
    10621 ggaagtaacg atcttctcat tgaccgaaga caataatttg attccttctt ctaatgtgtc
    10681 taatgtcttg actgcatatg gataaaattc ttctgcataa acggtcgtct caatgtttct
    10741 gccgctatgg taaaacaaag ggacacctaa ttcgttttcc aaatgtttga tcgcacggct
    10801 caaggcaggt tgagaaataa ataattcttt ggctgcttgg gtaacatttt ttgtttcgaa
    10861 tactttttta aaatactgca gatgaagcaa tttcataatt atccatccca tccatacctt
    10921 tttgttatgg ttttttcttt ttttggcatt ttttattata gcagaaattg actaagatag
    10981 cagtggaaaa agtaagtgct gaggaggcga ggctcatgag tacaggtttc ttatttcctg
    11041 gacaaggcag tcagaagtta gggatgttag cggatatccc tgaaatgtac atggaacgtt
    11101 tagaaaatgt gacggggtat cggttagttg ataaagaaga aaactatcaa gatacggttt
    11161 ttatccagct agcattactg acaaaagcag tattttattt agatgaaatg gaaaaaaacg
    11221 gtattcagcc tgatattgca gcaggacatt cgattggtgc tttttgtgca gctgttgctt
    11281 gtcgatcttt gagatttgag aatgcagcgg aactagtcta tcaccgcgca gtcttaatga
    11341 aagaagctta tccaagcggt tatgcaatgg gcgtagtagt aggtgtgacg agaagcgaag
    11401 ctgaggaaat tgtagcaaaa acatttgatc ctgaccatcc agtttatctg tcaaatgaaa
    11461 attgtccgat gcaacatacg ttatctggga gtatcgaagg tttatccgtt acgttggcag
    11521 aagccaaacg gcaccaagcg caaacagcga agctgctgaa agtccctgtg ccatctcact
    11581 gtctcttgat gaatgatacg gtcaagaaat tcaagccgta tatgcagtct gtccatctat
    11641 ctgatgctag atgtttgtat ataaaaaata cggatggaag agccactcag gacgcagaag
    11701 agattcgtaa agatttgttg gaaaacctag ctttccctgt gcagtggaac caaatgatgg
    11761 acttggcaaa agaattagga atggatctga cgattgaatt tccccccggc aatactttga
    11821 caaagctgat tcatacaaaa tttggtgtag ataaagggat ccgtacgatc aatctagacc
    11881 aacatggtat tgatgacgcc acgtttctat atcaaaaatg gagatgacta attatggaaa
    11941 aaccaaaccg caactgggct tctaaacgcg aaaaaaaagc gcagcgattg gaaaaaatca
    12001 gcaacttgct caatggcaag tatgtagaaa ctgaaaaaat cgtagaagta atggaaaaac
    12061 tgattgctcc cggcgacaaa gttgtattag aaggcgacaa tcaaaaacaa gcaagttttc
    12121 tttcagaaag tcttgaaaaa gtagatgcag aaaaagtgaa tgatctgcat ttgatcatgt
    12181 ctagtatctc acgaccagaa catttgaata tttttgaaaa aggaatcgca agcaagatcg
    12241 atttctctta tgctggtgca caaagcttgc gtgtcgccca aatgattgaa gacggcacaa
    12301 tgaaattagg tgatatccat acttatttgg aactttatgg acgcttattc atcgatttga
    12361 ttccaaatgt tgttttagta gctgcagata aagcagataa agacggcaac ttgtacacag
    12421 gtttcaatac ggaagaaaca ccaacaatca tcgaagctgc agctttcaaa gatggtatcg
    12481 tgatcgttca agttaacgaa gttgtcgatt ctttaccacg tgtagatatc cctggtgact
    12541 gggtagacgt tgtcgtgaaa gcagataaac cttatcaatt agaagcttta ttcacacgag
    12601 atcctcaaaa catcacagaa ttgcaaattc taatggcaat gatggcaatc aaaggaatct
    12661 acgcagaaca cggcgtacaa tctttgaatc acggtattgg attcaataca gcagctatcg
    12721 aattgttact gccaacttat ggtgaatcac tagggttaaa agggaaaatc gcgaagaact
    12781 gggtattgaa cccacatcca acgatgattc cagccatcga atcaggttgg gttgaaagca
    12841 tccatagttt tggtggcgaa gttggtatgg aaaaatatat tgctgctcgc ccagatgtct
    12901 tttttgtcgg taaagacggc acgatgcgca gtaaccgaac attatcacaa gctgcaggac
    12961 aatatgccat cgatatgttt atcggttcaa ccttgcaatt agattacgaa ggaaactctt
    13021 ctaccgtaac aaaaggacgt ttatctggtt ttggcggtgc gcctaacatg ggacataatc
    13081 ctggcggtcg tcgtcattct acaccagcat ggcaatcact tcgcgacaat gatgatccat
    13141 tagggaaagg ccaaaaatta gttgttcaaa tggttgaaac atatggttca aataaaaaac
    13201 cagtctttgt cgaagaatta gatgcactag ctgttcaaaa acaagcaggc ttagccaatg
    13261 caccagtgat gatttatagc gaagatacta cacatatcgt gacagaagaa ggaattgctt
    13321 atttgtacaa agcaaaaaca aaagaagaac gacaagcagc aattgcagca attggcggtg
    13381 ttacacctct tggaatgact agcacgaaag aatcactaga tagcttgcgt gcagccggca
    13441 tcgtgaaatt accagaagat ctaggcatca aacgtagtga agcgaaacgt tcattattag
    13501 ctgcacaaac catcgatgat ttgatcgaat ggtcagatgg cctgtatgaa ccaccaatga
    13561 aattcagaac ttggtcataa aattggcgag aaaaaccttt tgtcgccatt tacccagatg
    13621 gaagaagaaa gacgaataag cagaacgtgt atgctctgtt ttacaaaaag gggagagatc
    13681 catggaacaa ttagcttata aatatccaac gaaacagccg atcaataaga aaatccatgt
    13741 aggagtggtc ggttcaggag atttggaaat cttgatgttt ccaacagaaa aaacagaatc
    13801 tactgtaaaa atttgtacag gaagcgatgg cttcggcgaa gtatggaaca atgtattaac
    13861 gagatttttt gatcgttatc caatttcagc agatatcgtg atcaatgact tcggtgctac
    13921 accaggagtt gtgtacctac gattgacgca agcaatggag gaactttcag atgaaaaata
    13981 gttttgtaga attaaacgcc agagaacgag tgaacgctct acttgacaat ggacgtgaat
    14041 tgattgatcc attcgatcaa atcatcgcgc caagtttacc agcacaagga atcgtccctg
    14101 aaagtgatga cgggatcgtt gtcagccgtg gcacgatcaa tggcaaacaa gcagtagtaa
    14161 tcgctatgga aggaaaattc caaggtggcg gtatcggtga agtttccggt gcaaaaatcg
    14221 ttgctgcact tgaacatgtg ttagaagaaa ataaaaaagg aaatgagatc tttccagtca
    14281 ttgtactaga tacaggtggt gttcgtttgc aagaagcaaa ctacggactg ttatctattt
    14341 cagaaatcgg aaatgtaatc attgctctaa aacaatatgt accagtcatc ggtttgattc
    14401 caggacgtgt aggatcattt ggcggtatgt cgattacttc agcattaatg agttatttaa
    14461 tcgcaacaaa aaaagcgaga gtcggtttga atggaccaga agttattgaa caagaagcag
    14521 gcgttcgaga atttgattct agtgacaaag acttgatttg gaatacatta ggatctcgtc
    14581 aacgggtaga atcaaaaatc attgatgaat tagtgactga tagtgtagaa gcaatcaaag
    14641 ctgctgtcat cgcagcaatg gacgaacgaa aagacacgca tcgcagtgaa agctcagcat
    14701 tttatttatc attacttgat tctttagatc tttcaaaacc aatggccatt gacgattaca
    14761 atgaagcaat tgctaaccat caagcaaaat caattgactt accagaagta gcagaaggaa
    14821 atgaaccagc tgcagcaagt gttggttacg attggtttgc ggcactaact ggcatgaaaa
    14881 atccaacttc ttctattagt actgtgtatt atggtaaatc aagtaaattc atagaagaag
    14941 ccatcgtgac atcaatcgta cctgatccta aaaacccgat gtaccgtgta cgaaacggtg
    15001 aagttggttt agttgaaggt ttccgcatgg gacaaatttt ggatcacgtt tacgaagaag
    15061 ataaagacaa aaaagtgaaa cggccaatca tcgttgtgat cgatgtacca agccaagcat
    15121 acggctataa cgaagaattg atcggtatcc atgtggcttt ggctaatagt gctgctgctt
    15181 atgcaaaact ccgtcaagct ggacatccag ttattggatt gattgcagga aatgctattt
    15241 caggtgcatt tttagcacat ggattacaat caagccgctt gattgcttta aacaataaag
    15301 aaattaccgt ccaagcgatg tctaaagaaa gtgctgcacg tgtgacaaaa cgttcggttg
    15361 ctgaaatcga agcagctgct gaaaaagttc cagctatcgc ttacgatatc aataattata
    15421 caaaactagg agcgatctat catttcctag aagatatcac cgaccaagct gcttcagaac
    15481 aaaatgtcag caaagtcatc gaagcaatca atgaagccat tcaagatgtc cgcaatacaa
    15541 atgacacgat gctgacaaat cgatacacaa acgaaacagc caaaacattc ggacgtgtcg
    15601 aaacaaataa agtccgtgcc aaaatggacg aagaatggga tgcataaaaa atgattcaag
    15661 cacatgatat tgtccttttt gataaacatg aaatagaagg gttcaccttg cctgaatggg
    15721 tcgttctaac tgaaaagatg ttcggaacag ttcgtcgtgc gtcgacggat cacgcagaaa
    15781 tactaccgat tggtttacgt ggacctgccc gcaatcagag atggtcaaca gaaatctcaa
    15841 aacatgccgt caaacaagtc attcatccgt gggacatcat cgaacaagaa tcgtatagac
    15901 aagagaaaat catggattat cctgtttatc agcaatttca tgctgctaga aacatattat
    15961 caggatgtaa atggggaatc ggcggcagct taggatttga attatcgact gggattccgg
    16021 cagtaaaaga aaccagtgat tttgatttgc tgctgtacgc aaattcaccg attgaattgc
    16081 caattcaggt aattcaatcg catcctgctt tttttgagca attcgatacc caagtagtga
    16141 ctcggcaagg cggtttttca ctaaaagagt atttgagaga gccagagaaa aaaattctat
    16201 tgaaaacagt cgaaggacct aaattaacca aagaactttg gtaataaaga aaaagagaga
    16261 gaaagagatg tttgaaaaca aaaaaatcaa aatcaacgta agtgacttcc agaaagtatc
    16321 agctgtaacg gctgttatga tgcaaacgat attgagcttt gctttggcaa acagccacag
    16381 cgcaacaaat acagcttttc ttggtacctt ttacgtcatc gttaaataca cagcaccaat
    16441 gtttattttt gcaattgttt ataatatggt aaaaacaagt cagcatttgt cttattggga
    16501 atttttgaaa gaaaagtttt ttgaactagt cgttccgtat cttctatgga cgacggctta
    16561 tctttggcta tttccagcag tccaacaaca aactccgtat acggatactg caagcttttt
    16621 actgaaatat gtcacaggtg atggagcgcc tcatttatgg tataccgtta tgatgctgca
    16681 gatccaattg ttcatgccgt tttttgtttg gctcggttac aaagtatttg ctaataaaaa
    16741 atatgtatgg ccagtattga ttggttcaac gattttatat gtcgcatggt atctgttcta
    16801 tcaaacacaa gtattgactg ggccttatca tgacagctgg tacttattag atcggtttgt
    16861 tgctagtttc atgatttatg gggtagccgc atttgtttat cacgaaaaag tctatcaata
    16921 tttggacaga gtacgttatc ttttcttacc tgttgcctta gttatcgcgt tcttttctgt
    16981 ccgttcattg ctcgcacatc caggagactt gagctttgcg aatgcacctt acttgaatac
    17041 gatccaaagt ttatacagtt tagtcatcat ctttgctgtc ttcataggcg cttccaaaat
    17101 gattgtaaac gattcgccaa aattgccatt gttcaaatgg ttgtctgttt atgcttatcg
    17161 tacgtattta gccaatgtat ttgttttcca agtactgtta ttattgttta aagatagttg
    17221 gctgcagtta ccttcaggag tcatgattct tgtagcttat ctaatgacgg caagttgtgc
    17281 atttgcttta agctggttac ttcatatcat ttgggtagct atcaaaaaag gtttcagcaa
    17341 gtaatagtag gaagaaggag tgagtgtcat gataaatgac gaacgaaaaa aagcagtgac
    17401 tcagcaactc gctgcgttgg tagaggaagc attgatcgca gaagtaacat taagtccaaa
    17461 acctggatta gtggatgcac tggatgcggg tgcacattcc gatatggact atgctttatt
    17521 tcttaagtct gctaagacgc taactccttt ttttgaagaa atggctcaaa tagcatggca
    17581 tcatgctatt gatcaggagt taagagaaag aattgctgag attgggcgaa aagcggaaaa
    17641 ggcaatgctg attgctacaa atggcatcaa tacccataaa ggagcgattt gggcaatggg
    17701 gctattgatc agtgtcattg cacaaaaaat cagtatggaa cagccttttt ctttatcgaa
    17761 aatacttgat gatgtcagca agcttgctgc ttttcctgat acaaaatatc gtcataaaaa
    17821 aaccactcat ggtcataggg tgaagcaaaa atatggcgta aatggtgcat atgaagaagc
    17881 gatattaggg tatccgcata tcaggcttgc aattcaagca gctaaggagc tagataatga
    17941 ttcatatgta cagaaacaaa tgcatatgtt gttagttttg atgagttcgc tagatgacac
    18001 atgcgtattg catcgcagtg atcagaaaac gttagtggaa atgcaaaaac ttgctcatgc
    18061 tgctagtcaa agtgctttac caaatcgtcc atttacagca ctgttgcact tttgtcagga
    18121 aaaccaaatc tctcctggag gaagcgcaga tctcttgtct gcaagcttgt tcatgctgaa
    18181 gatagaggaa ttatggactg ccgacacata catttgtccg atgttactca atgactaaat
    18241 aagacaaaat tgctaaaaaa acagtttatg catctggtta ttgactcttc cttttagcaa
    18301 ggctatcata aaaatagttt tttgtaataa aagataacga ggaagacaat gaaaaaaatc
    18361 aattatttag caatcatttt gttagtgggc ctatgtttgt ttgctctgcc gacccaagga
    18421 ttcgctgtcg cgggtggtca tggtggaggt acatctagcg gtcgaagttc gggcagaggt
    18481 tcatttggtg gtcgttcata tgggggcagt tattttggca gtcgttctta cggcagagat
    18541 tcttatgggt attatggtcc aggtacaggt ccttctagtt atgagcaaac gggatttctt
    18601 gcattagctg ttttgtcagt aggaggggtt tctgcttttt ctatcgtcaa gaataaaaga
    18661 caaaagaaaa ggctgaaagc ggagatattg tcccgcatgt caggaacggc taaagaaaag
    18721 aaagcacagt ttctggaaat ccaaaagata ttcatgaaga tccaatccgc ttgggaagaa
    18781 gaaactcctg aaaaagccag aagctgttat acagacaagc tgtttgccga acatcagaaa
    18841 gttctgcaaa aaaacaaagc agacggtgta agaaatcata ctaaaaaaat aactattcag
    18901 gaagtaggaa attatcgaca gatccacgaa aatagttttg ccatccgaat tgatttcaga
    18961 tgctgtgatt attcagtcga tcgaaaagat gggcgattga tctcaggaag taagacacaa
    19021 cgacagtatt tttctcagat atggtatttt gatttttctg agaaagaaaa aagatggcaa
    19081 gccgatttta ttcagccgat tatgctggat tgacaggaaa tatttatgaa cggagttgtg
    19141 acaaaagcct tgtcgcagct cctttttctg aataaacggt gtgccagaga tagcctatcg
    19201 gcaataaggc acgattttgt aaaatatggg aagcgatttt tcaaaatcgc ttcttatcgc
    19261 tcaaggctga acatctctgt cacaacctct ccactaaatg gtgtttaaaa gcttgcatct
    19321 gcgataataa gaacaaccaa acgaaaaata ggaagcacta ttttagttta gttgttctta
    19381 ttacttgatg caggacagct ttttcacaac ctcttttttg tatagatggg caaaattttt
    19441 gtaataatag gcgtatgtct ttttcgttga ggagatgaaa aagcatatgg cttatttttt
    19501 gtttgctttg cttactttct ttccacttca agtggcagaa atatctactt tttattatgt
    19561 aagcggaacc atatacttga aatcagaagg agatcatttc tatgaaaaca agaacaattg
    19621 aaatacttca cagaatcatg acagcccaat atccagtaaa agcaaaacat ctagcaagtg
    19681 agttcaatgt cagtcagcgg acgatccgtc aggaagttct tgaagcgaat atctggttga
    19741 gcagtaaaaa gcttccggag atccgtacaa ttcgcaacaa aggttttttg ttgaagctgg
    19801 aaaaggaaaa gcaagctcga ttagaaactg aaataaaaga tgtgaaagac ggctttttga
    19861 atcggcgcga acgtattttt gatctagttt tagccgttgc ttacagccga atcccgatcc
    19921 atctaaacaa aaaagaagaa gaatttcaaa tctctaaaag tacattagat gaagatatga
    19981 gacggttacg agcaacactg gtcagttatg ggatagagat cgtcagttat ggaaagcaag
    20041 gactggttta taaaggttca gaacgatcta tccgtacgat gatctatgat tttatcaatc
    20101 agaatctggg aatgaccgac ttctttatga atcaagatgc acaattaaca ccggctcaaa
    20161 aaatctttct ccattatttt cctttggtgg aaatcaaaaa aatacaggag atctattcca
    20221 ataagttgtt gaaacaagaa gatgatattt ataaaaaaca aatcctgctt tttacattga
    20281 tctggatcca gcgaatcagg aaacatgaat ttatctctgc cattaattgg aaaaatacag
    20341 aaataaaaga cagtagcttt tctgcattta ttgaccacat gatcgaggaa tttcaattgg
    20401 acaatgtccc gcaagtagaa agaaattatc ttcgtttcac tattgagacg ttcaatgcta
    20461 gagatatctc caattcaatc gagtgggtac aagctcaatt actaacgatc caattgatcc
    20521 aatttgtaga gaacgagaca cacgtacctt tccatctaaa ggaagaagct ttattcgaaa
    20581 atctctataa gcacatggct gcactgattg taagaatcaa gaatcatatc caagtcatga
    20641 atccattgaa agaaaacatc cgaacaaatt atttcccaat ctaccgcgca gtcactcatt
    20701 ttgttcctac gatcgaagaa gtaattggtg gcaagatact ggaggacgag atagcttttc
    20761 ttgtcatcca cttttcgacg atcgccagtg taatcaagcg agacttgaat tatgtttata
    20821 aatcagtcgt tgtctgtaac catgggatgg ctactggaaa tctgttagca gagaatctga
    20881 aagaaaagtt tcctcagatc gagattactg cagttttaag ttcaaaagaa atcgaattag
    20941 tagataaatt ggatgtcaat ctggttttca gcacctttca attgaattat cgaaataagc
    21001 cgcttttagt tgtcgatccg attttaactg agacgaatat cttggttata gaagactttt
    21061 tgaaaagaaa tgaagaacta cagcggttag agcctaacag tcatgaatcg acggagttat
    21121 tcactcaaat aatagatgtc atgaaagaaa gtggtggccg agtagatcga atcatctatg
    21181 gcaagttaga aaaattattc gaaacaaaca atttagaaat caacaaaagg gagatccagc
    21241 ctatgttaaa agatattttg acggacagca atatcatgat caaagaaaaa gccactactt
    21301 gggaggaatc gattgaacta gctgctagac cattggttaa agaaaacatt gtcgaagagc
    21361 gctatgtcga agcgatgatt cgtgcagtga atgattacgg accgtatatc gtgattggca
    21421 aacacatggc actagcccat gcccgaccag aagatggggt caataaatta ggagtcagcg
    21481 tagctacgat tgagcaacca atcgatttcg gtaatcctga aatggatcca gtaaagatta
    21541 ttttttgttt ggcagcagta gactcctact cgcatctgaa catcatgaaa gaactaattg
    21601 aattgatcaa caacgaagag aagttggatc gattgattga atgcacaaat atcgaagatt
    21661 tcaaacaact gcttttttca gtagttgaga aataataaat gatacggggg agaacaaaga
    21721 tgaacaaatt gaacatttta ttcgtatgcg gtgcaggttt aggaagtagt tttgcagcac
    21781 agatgtctgc tgaggatgtt ttgaacaaat taggagtaga agccaaactg gaccattgtg
    21841 atatttcttc cgcagtttcc atgaaaccag atgtgatcat cacagcagaa aattttcgtt
    21901 cgcagttcga aaagttcgca gttgatccta aaacggcgat gatctatttg aaaaatattg
    21961 tttcaaaaaa tgaaattgaa gaaaaaatca ccccagttct gcaagataag ggagttatct
    22021 aaatacaaaa agaaaagagg gtaaaaacat gggcgttatc aattttatca tcgaaaatat
    22081 tttgactcag gcatcgatca ccattgcatt gattgctatg ttgggtctac tactgcagaa
    22141 gaaatcagca ggacaagtca tctctggcac gttaaagaca ctcctaggat ttcaagtgtt
    22201 gagtgctggt tcgagcatca tcgttggaag tttaacttat ttcgggaaaa tctttactga
    22261 aggatttcat atgcaaggga tcatcccttc tattgaatct atcaatggtc aagcaatgaa
    22321 tgatttgggt ttaggtagag atattgcact gacgttctta gccatttttg tattcaatat
    22381 tattttggct cgtttcacaa aatggaagta cattttttta actggtcaag caattttatg
    22441 gatggcaacg atgacgacag tctttggcta ttttgccggt ctgcgaggga tcgtattgat
    22501 cttggtcggt ggttttatcg gagcttgttt cgccattgct atgccggcag tagcgcaacc
    22561 gatcattcgt aaaatcactg gatcaaatga tattgcttta ggtcattttt gtacgatcgg
    22621 gtacttattt gaagccggtg ttgctaagct ttttggtgag aaaggcgaaa ataaaaagtc
    22681 gatcgaagat atcaagttgc cgacacattt cgaatttctg caagatactt atttgtctgt
    22741 catggtcgtg atggtcccgc tatatatcat cactgtttta tttgcaggcg aaccattcgc
    22801 ttctgaatta tcaggagatc aaaactatat catgtttgcc tttttacaag ccatccaatt
    22861 cgtagtgggt gtgtatgtat tgctagctgg tgtacgtcta ctgttaggtg aaatcgtgcc
    22921 ggcatttcga gggatagcta tgaaattagt tcctgatgcg atccctgcct tagattgtcc
    22981 agttttcttt ccttatagcc caaatgctgt cattttaggt ttcattacga caacgattgg
    23041 tacgattatt gccatgttta ctttgcccat gttcggctta gcgatgattt tacctggtat
    23101 gctaaccaat ttctttgccg gagggacagc agggatattt ggcaacgcag ttggcggacg
    23161 ccgaggagcg atcattggcg gtattgctca tggtttcttt attacattat tgcccgcttt
    23221 gctagtaacg atctttaatt cgatgggatt caacaatgct acagcaaccg atgtagatac
    23281 agtagccgca gcactcttat atgcctggat cttaagccca gttttgaaag cattttaatt
    23341 gaaagtaggg aaaatgatgt ttggattttt taagaaaaaa gaaaaagaac cgaaaaaagt
    23401 tctggcaaaa gaagaaaaag ccctatcgcc agaagcgacc gaaaagattc aatcagagat
    23461 tacacaattg aaacaagaga tttccactac ccaagataag cataaattag cgaaacttta
    23521 tgaacaagtt ggacttaaat tttctgaatt gtatgtgaac gatcaagcca tccaatattt
    23581 agaaaagagt ttggaaaata aacagacgat cggagatggg tataaaaaac tgatgagcct
    23641 ctataaccag aagcgtgcag atgctgctcg tgctggtgat gatcaaggga tcgattacta
    23701 catgggcaaa atggatgaga tgcgacaaat tgcaaaacaa gtaacgatca aaggaaacaa
    23761 ataaaaagat aaggtggcaa taaaagatgt atacaacatt gaaagaagta acaaaaacag
    23821 cagaagagtt gaattttaca gttggagctt ttaatacgca taacctagaa atgcttcctg
    23881 ctatgctagg cgcagcaaaa gaaatgggtt cgccgatcat tattcaaaca agtgtcgata
    23941 ccgctaaata tatcggcatg catgtgttag tcaatgtaat gacagctatt gcagaagaag
    24001 aaatggttga tgcagtcttg cacttagacc acgcccgaga ctttgatgat atcaaagaag
    24061 caatcgacag cggatatact tctgtcatgt atgatgggtc ccatctgcct ttcaaagaaa
    24121 atatcctgaa gacaaaagca gtagtagaat atgcccatgc gcacggtgtt tccgtagaag
    24181 gagaacttgg aacaatcggc ggtacagaag aaggaatcca tgtcgctgaa aatgacaagg
    24241 tatatacaag accggaagac gcagtgaaat ttgtagaagc tacaggcgtg gatgcattag
    24301 ctattgctat cggtacgaat catggacaat tcaaatcaaa aacagaagta aacattccct
    24361 tgctgaaaga aatcgatgca gtagttgatg tccccctagt tatccatgga ggaacaggtg
    24421 tgaaagaaga agattatcct gaattgatca ataacgggat ccgcaaattc aatgttggaa
    24481 cggagctttt agtcaactgg acgaaagtag ctaaagaaac ctttagtgaa acagaaatca
    24541 acaagtctct tcgccataat gtgattccag caaatgaagc agtaaaagaa attgtcaagc
    24601 ataagattgg actgtttatg aacttggaaa gtccaattaa cttaggaaat aaatgagatg
    24661 aaaaagtatt tcattcttct ggctggtagt cctggtacag gaaaaacata tcttatgaat
    24721 aaactacggg aacgatttcc agatatgtat gccatcacgc tcgatgaaat caaggaatac
    24781 tatgcagaat ccatcgggtt taacaatttg gaagaacggg cggaacaaga aaggacgaaa
    24841 gtttatcctt tctattacaa agcacttgat ttgtatatgg aagcaggaaa gaaagtgatt
    24901 gtgtccgagt atccttttag tgataagcaa aaagcacgac tccaagaatt agcagataga
    24961 tacggatatc aggtgatcac gattcgccta acagcagact ttgaagtgtt atggcaacgt
    25021 cggtacaaac gtgatcgtga atcagatcgt catttgagct atatcatgga tcattatcac
    25081 tatggtgacg tattggaaga tcgaacgaaa gggaccaatc atattacgaa agaagcattc
    25141 aaaaagatca tcgccgaccg aaaatatgac aaattccagt taggaacact atatgaattt
    25201 gatgtaacag actatactca agtggattac actccactat tggataaatt agaaaaagaa
    25261 atcaatgaca ataagtaaca aagaaatggc ttaatatcga aaaattggct tttgaacgct
    25321 gtttaccgag aggtcgtgag cctgtcgttc agctccgagt cacaaggagc aatttcaaaa
    25381 aaacagcttc tcatgtttga tgaatattgt gacttgtgac cgaggagttg gtaggagaac
    25441 accgtttagt ggggaggttg tgaaaaagct gtcctgcctc aattaataag aacagccaga
    25501 caagaatagc tcgtcatatt tttcgtttgg tggggcttat taccgcagat gcaagctttt
    25561 gaacgccatt tattcacagc cccagagaag tcgaatgtct tttgttctcg agttttttaa
    25621 cattctttta tttattgcag atttcattat atttatttgc cgtaattaaa ccagtcttct
    25681 atacttgatt acatagtcat acatttgcta aaaaagaagg gaagagaatg aacaagaagt
    25741 tggctcaata caaaagacta ctcgcacttt ctcaagctgg tctatattat ggaaaagatg
    25801 tatttgataa agagcgtttt gaggagatcc ggacgatttc attggaactg atcagtgaaa
    25861 taggaaaaga aactttcgaa gaaataaaag cgctcacaac gattggagaa ggacatccaa
    25921 caccaaaagt agatgttcgt gcctatatca aaaaagatgg caaggtccta ctaatcgaag
    25981 ataaacggac gaaagaatgg tcacttcctg gtggatttgc tgaaatagga ttgtctcctg
    26041 aagaaaatgt gcgtaaagaa gtctatgaag aaacaggatt aacagtagaa actactcagt
    26101 tgcgagcagt ttttgatacg aataaacaaa aagatattcc acagcttttt cagtattata
    26161 aacttgtttt tgcttgtgcg atccacggtg aagaggccaa gtttattgag aataatgaaa
    26221 cttccaacat gggatttttt agtatagaag agttgccaaa gctatcagaa aagcgaacaa
    26281 cgaagaaaca gttacttatt ttagaaaaca aaaaccagat ctattgtgat tgagcttttt
    26341 cgtaaaagaa gaaaagaata tactttacta gttattaaaa aagaacttga tttttttatt
    26401 gaaaatagtt gcatttgcaa caaaaagagg tgaactagtc ttacaaacaa aataaaggtg
    26461 ggatgaaatg gctgaaatcc ttcgagacat cggaatgatt gcccgagcgc tagattctat
    26521 tagcaatatc gaattcaaag aagtcgattt aacaagagga cagtatcttt atttagtacg
    26581 tatttgcgaa aatcctggaa tcatccaaga aaaactagca gaaatgatca aagtagatcg
    26641 tacaacgact gcccgagcaa tcaagaaatt agaatccaat gggatgatcg aacgtttgga
    26701 agataaagaa aataaaaaaa tcaagaaact ctatccgact aaaaaaggag cagaaatcta
    26761 tcctttcatc attcgagaaa acaactactc caatgcagtc gcattaaatg gtctttctga
    26821 cgaagaagca aaacaattgg aatatctttt aaataaagta tgcaagaatg ttagcgaaga
    26881 ttggaatttt gtcaaaaaag gcaataaaag gatctattga aaggaagaag ccgtgaataa
    26941 atacgaaatt cgaaaaatga cgccttcaga tgtagactta ctacaacaaa ttagtatcga
    27001 aacatttaca gatacatttg gaaaagataa ttcaccggag catttagctg attacttgac
    27061 acaagcttat gcactagaaa aacttacaga agagctaaaa gataaagaaa ccgatttttt
    27121 cctagtgagt atcgatcaag agcctgcggg ttacctgaaa ctaaatacag gaacgagtca
    27181 atctgaggaa atggctgatc attttttaga aatcgagcga atctatgtac gaaaaaaatt
    27241 taaacatcaa ggtatcggta catttttact agatacagct attgaaaaag caaaagaaaa
    27301 gcaaaaaaca gccatttggc tcggtgtttg ggaacacaac gatgttgcgc tcgcttttta
    27361 tcgtaaaaaa ggatttatca aatacagcga acatgttttt tatatgggag atgatcccca
    27421 aacagactat ctattcatca agcaattatt ctaatcaaga ggtgtgaaca gatgaaaaaa
    27481 gtaatagtgg ataccaattt ctgggaagta tttcctgaag caaccatcga aatcttatcc
    27541 gtaaatggta tcgataacca cgtaacaaaa gaaagtgaag aaacgtatca tcaattattg
    27601 aatgaggcag caaaagaagc aagaaattac cttacagaag aaacattcag ccaaaacgaa
    27661 gtcattgctc agtggcgtca ggcatttacc acattcaaga ctaaaaaagg tgcacgttct
    27721 tctattgaag ctttactgaa acgtgcaaat caagaacggg aatttttccc aatcaatccg
    27781 cttgtagata tctataatag tatttccttg tcttttggcg taccttgcgg cggagaagat
    27841 atcgaaaaga tcgcaggaaa tctgcattta ggaaaagccc aaggtggcga atcattcctg
    27901 cctttaggtg cagattccga tgctccggca cttccggaag aaatcatcta ctatgatgaa
    27961 caaggagcga tctgtcgctg tctcaactgg cgtgaagccc aaagaacgat gctgacggaa
    28021 gaaacacaag cagcggtgct ggtcattgaa tccatcaatg ctgaacaagc ggcacgtggc
    28081 gcacaggcaa tcgaagcgtt gaaggaaaaa atcgatcaaa catttggcgt aactagtgtt
    28141 agacacacac taaccaaaga tgaatcagaa gcagaattat aaaagaatcg aaataa
//
LOCUS       DAXWLF010000017        28125 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXWLF010000017 DAXWLF010000000
VERSION     DAXWLF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.017"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(51..419)
                     /gene="rplL"
                     /locus_tag="ACGE4U_000559"
     CDS             complement(51..419)
                     /gene="rplL"
                     /locus_tag="ACGE4U_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFF8392747.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(472..975)
                     /gene="rplJ"
                     /locus_tag="ACGE4U_000560"
     CDS             complement(472..975)
                     /gene="rplJ"
                     /locus_tag="ACGE4U_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFF8392748.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(1179..1306)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1385..2074)
                     /gene="rplA"
                     /locus_tag="ACGE4U_000561"
     CDS             complement(1385..2074)
                     /gene="rplA"
                     /locus_tag="ACGE4U_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFF8392749.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(2178..2600)
                     /gene="rplK"
                     /locus_tag="ACGE4U_000562"
     CDS             complement(2178..2600)
                     /gene="rplK"
                     /locus_tag="ACGE4U_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFF8392750.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            2943..3611
                     /gene="sdaAB"
                     /locus_tag="ACGE4U_000563"
     CDS             2943..3611
                     /gene="sdaAB"
                     /locus_tag="ACGE4U_000563"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFF8392751.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            3624..4496
                     /gene="sdaAA"
                     /locus_tag="ACGE4U_000564"
     CDS             3624..4496
                     /gene="sdaAA"
                     /locus_tag="ACGE4U_000564"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFF8392752.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(4587..5687)
                     /locus_tag="ACGE4U_000565"
                     /pseudo
     CDS             complement(4587..5687)
                     /locus_tag="ACGE4U_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(5779..6666)
                     /locus_tag="ACGE4U_000566"
     CDS             complement(5779..6666)
                     /locus_tag="ACGE4U_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8392753.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(6743..7288)
                     /gene="nusG"
                     /locus_tag="ACGE4U_000567"
     CDS             complement(6743..7288)
                     /gene="nusG"
                     /locus_tag="ACGE4U_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFF8392754.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(7389..7559)
                     /gene="secE"
                     /locus_tag="ACGE4U_000568"
     CDS             complement(7389..7559)
                     /gene="secE"
                     /locus_tag="ACGE4U_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFF8392755.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(7583..7735)
                     /gene="rpmG"
                     /locus_tag="ACGE4U_000569"
     CDS             complement(7583..7735)
                     /gene="rpmG"
                     /locus_tag="ACGE4U_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8392756.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            7897..8541
                     /gene="cbpA"
                     /locus_tag="ACGE4U_000570"
     CDS             7897..8541
                     /gene="cbpA"
                     /locus_tag="ACGE4U_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFF8392757.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(8597..9100)
                     /locus_tag="ACGE4U_000571"
     CDS             complement(8597..9100)
                     /locus_tag="ACGE4U_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFF8392758.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(9113..9159)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            9697..10662
                     /locus_tag="ACGE4U_000572"
     CDS             9697..10662
                     /locus_tag="ACGE4U_000572"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8392759.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(10761..11933)
                     /locus_tag="ACGE4U_000573"
     CDS             complement(10761..11933)
                     /locus_tag="ACGE4U_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8392760.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(11920..13065)
                     /locus_tag="ACGE4U_000574"
     CDS             complement(11920..13065)
                     /locus_tag="ACGE4U_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8392761.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(13079..14011)
                     /locus_tag="ACGE4U_000575"
     CDS             complement(13079..14011)
                     /locus_tag="ACGE4U_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8392762.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            14128..15207
                     /locus_tag="ACGE4U_000576"
     CDS             14128..15207
                     /locus_tag="ACGE4U_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFF8392763.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            15204..15833
                     /locus_tag="ACGE4U_000577"
     CDS             15204..15833
                     /locus_tag="ACGE4U_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8392764.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(15943..16170)
                     /locus_tag="ACGE4U_000578"
     CDS             complement(15943..16170)
                     /locus_tag="ACGE4U_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8392765.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(16273..18861)
                     /locus_tag="ACGE4U_000579"
     CDS             complement(16273..18861)
                     /locus_tag="ACGE4U_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFF8392766.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(18881..19540)
                     /locus_tag="ACGE4U_000580"
     CDS             complement(18881..19540)
                     /locus_tag="ACGE4U_000580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8392767.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(19589..20092)
                     /gene="trmL"
                     /locus_tag="ACGE4U_000581"
     CDS             complement(19589..20092)
                     /gene="trmL"
                     /locus_tag="ACGE4U_000581"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFF8392768.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(20313..21179)
                     /locus_tag="ACGE4U_000582"
     CDS             complement(20313..21179)
                     /locus_tag="ACGE4U_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFF8392769.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            21288..21929
                     /locus_tag="ACGE4U_000583"
     CDS             21288..21929
                     /locus_tag="ACGE4U_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFF8392770.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(21991..23352)
                     /gene="mgtE"
                     /locus_tag="ACGE4U_000584"
     CDS             complement(21991..23352)
                     /gene="mgtE"
                     /locus_tag="ACGE4U_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFF8392771.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(23370..24269)
                     /locus_tag="ACGE4U_000585"
     CDS             complement(23370..24269)
                     /locus_tag="ACGE4U_000585"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8392772.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKSYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(24271..25068)
                     /locus_tag="ACGE4U_000586"
     CDS             complement(24271..25068)
                     /locus_tag="ACGE4U_000586"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFF8392773.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(25046..25750)
                     /locus_tag="ACGE4U_000587"
     CDS             complement(25046..25750)
                     /locus_tag="ACGE4U_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFF8392774.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            25881..26456
                     /locus_tag="ACGE4U_000588"
     CDS             25881..26456
                     /locus_tag="ACGE4U_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFF8392775.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            26574..27251
                     /locus_tag="ACGE4U_000589"
     CDS             26574..27251
                     /locus_tag="ACGE4U_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFF8392776.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(27341..>28125)
                     /locus_tag="ACGE4U_000590"
     CDS             complement(27341..>28125)
                     /locus_tag="ACGE4U_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFF8392777.1"
                     /translation="SVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLAT
                     REAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGN
                     SHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQ
                     GYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYS
                     NGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT         8200 a         5915 c         5083 g         8927 t
ORIGIN      
        1 aaaagcaccg aaatccaaca taggatctca gtgcttttaa atagtaaaaa ttatttaaca
       61 gttacagatg cgccaacttc ttcaagttta gctttcaatt cttcagcttc ttctttagat
      121 acgccttctt taactggtgc aggagcgcca tcaactacag ctttagcttc tttcaagcct
      181 aaaccagttg cttcacgaac tgctttgata actttaactt tttggtctcc agctgcagtt
      241 aattctacag tgaattctgt ttgttcttca gcagcagcag cgccaccagc agcagctaca
      301 gctacaggag cagcagcaga tacgccaaat tcttcttcaa tagctttaac taggtcgtta
      361 agttctagga tagttgcttc ttttaactca gcaacgatgt tttcaatgtt caatgccatt
      421 ataatttcct ccgtttaatt gttctttttt atttaatgct ttatgcggca attaagctgc
      481 atctccatct tctttgtctg ccactgcttt gacagcgtaa gccacgttgc ggactggcgc
      541 ttgtagtaca gatagcaaca tagatagaag tccttcgcga tttggcaatt ttgccaatgc
      601 tgtgatttct tccaatgaag agactttacc ttcgatgata ccgcctttga tttctaatgc
      661 ttttgcttct ttagcaaatt cgtccatgat tttcgctggt gcgactacat cttcgttact
      721 gaatgcaaca gctgtaggtc cagtgaatac ttcgtccatg ccttcaaggc cagctttttt
      781 agctgcacgt gaaagaatag aatttttgat aactttcatt tctacgttag catcacgaag
      841 ttgtttacgt aaacgtgtta cttcatcaac tgttaatccg cggtagtcaa cgactactac
      901 tgatgcagct gctgtaaatt tttcgcttac ttcatcaacg attgctgctt tttttgcgat
      961 aattgcttca ctcatttttg agtttcacct cctgaatttt gatggaagag ttttttgcac
     1021 tgaaaaggca agtcactttt atagttactt tgctcaacca gtattattca tcacctattc
     1081 acttcgttca tagtgattct actcgctttg ctcgaaagga aactcctttc gctttgctca
     1141 accagtattg ttcatcatct cttcacttcg ttcatagtga ttcacaataa aaaactctat
     1201 gccaccgcag acatagaggg actattgaat atattcaata aacgtcctcg gtaggaaatt
     1261 aaggcttgat gccacctact gtcttcggta caagttaatt attaacctca actagcttag
     1321 catgctaaac tagttgagtc aagaattatt tttcagttgg atcaatcaac attctaatat
     1381 ttaattagaa agaagcttgg tcaacatgga taccaggtcc aaatgttgtt gtgatcgtta
     1441 catttttcat gtattgacct ttagctgctg atggtttagc ttttagcaat acatcatgga
     1501 ttgctgcaaa gttttcaact aatttttcat cgtcaaatga aacttttccg attggtacat
     1561 ggatatttcc agctttgtcc acacggtaag ttactttacc agctttcact tcgtttactg
     1621 cttttgttac gtccattgta actgtaccag ttttagggtt tggcattaag ccttttggtc
     1681 ctaatactcg tcctagacga cctacagtag ccatcatatc aggtgttgca acaactacat
     1741 caaagtcaaa ccatccgttt tggatttttt gaaccatgtc gtcgtcacct acgaaatctg
     1801 caccagctgc ttcagcttct tttgctttgt ctcctttagc aaaaactaaa acagtttgtg
     1861 ttttaccagt tccgtttggt aatacaacag caccacggat ttgttggtct gcttttttag
     1921 ggtctacgtt caaacggtat gcaacttcaa ctgttgcatc aaattttgca atgtttgttt
     1981 cttttgctaa agctactgct tcggcaacag gataaacttt acttgaatca acttttttta
     2041 atgcatcttg catttttttg ctctttttag ccattttggt tcctccttga ttgtggttat
     2101 aacggttgta cctcccacgt gttctatatc tttcaatata gagccaactg agaagctgct
     2161 gtcttagggt gtatagatta ttctacagtg atccccatgc ttcgtgcagt tccttcaacc
     2221 atgcgcattg ctgcttcaac gttagctgcg tttaggtctt ccatttttaa ttcagcgatt
     2281 tctcttactt gatcgctaga aactttcgca actttatttt tgtttggttc gcctgaacct
     2341 ttttcgattt tagctgcttt tttcaataga acagcagctg gcggtgtttt tgtaacgaat
     2401 gtgaatgaac ggtcttcgta tacagaaata acaaccggaa tgatcaaacc tgcttgatca
     2461 gctgtacgag cgttgaattc ttttgtgaat cccatgatat tgatacccgc ttgacctagt
     2521 gcaggaccta ctggtggagc tggagttgct ttacctgcag gaatttgcaa tttaacgatt
     2581 ttttctactt tctttgccac gagacatacc tccttaagtc cgtgatgtgg ttaattggag
     2641 atgcatattt ctcctcccac gttttatctg atctgaaaaa atccagacaa ataatgtgca
     2701 aaacaatgca cactggtaca gtatagcaaa tttatttgtt ttttcaagat ttttttctgc
     2761 tatttttaca ttttgcttga ataaacgcta aatttattct aaaaaaacaa caatttttcg
     2821 ttctcatttc ttcttttatg ttacacttgc gctagcatgg ttcaacagat gaaagcaaac
     2881 aataacacgg ttttttagac ccttgtacaa agattcctac ataacaaaat gaggtggaga
     2941 aaatgaaaga tttaaggtat agaagtgtct ttgatattat tggacctgtg atgattggtc
     3001 caagtagctc tcacactgcc ggagctgcta gaatcgggaa aatcatacgt agtatatttg
     3061 gcgaacagcc tgattctgta gatatttatc tatatgaatc atttgcaaaa acctatcggg
     3121 ggcacggaac agatattgca ttagttggcg gcctgttagg gatggaaccc gatgatgaac
     3181 gtttagctga ttctttgaaa ttggcatatg aagccggtat ggaagtcttg tttgttccta
     3241 agagtgaaaa agccgaacat ccaaactctg taaagatgct tctaaaaaaa ggaacacgaa
     3301 agctttcagt cactggcatt tcgatcggcg gtggcaatat ccagatttct gaattgaatg
     3361 gattcaaaat ttctttaagc atgggtactc ctaccttgat cgttgtccac caagatgttc
     3421 caggtatgat tgctaaagtg actagtatac tttctgagac caatacgaat atcggcacta
     3481 tgacagtgac acgggaatca aaaggtgaaa atgccattat gataattgaa gtagatgatc
     3541 ctcaagtaga agaaacggta aaaagactca aacagctgcc aaatatcgac agcgtaaatt
     3601 atttcgatta aggagctaaa aaaatgtttt tatcgattga agaattagta gatcaagcaa
     3661 aagcttatcc aagtgtagca gaattgatga ttgcagttga aatggaaatg agtggccgtt
     3721 cccgtagtca catcatcgag atcatggaaa aaaacctctc agtaatggaa caatcaatca
     3781 acgagggaaa agaaggtgtt acctccgtca caggaattac cggtggtgat gcaaaaaaat
     3841 tagatgatta tttaaatgag gcagactttt taagtggaga aacaatctta aaagctgtac
     3901 gcaatgctgt agctgttaat gaagtcaatg caaaaatggg gttgatttgt gctacaccga
     3961 ctgccggaag cgcgggtgtt gttcctggtg tgctgatggc tgtccgagat cggcttgatt
     4021 tgacacatga acagcaattg gaattcttat ttaccgctgg cgctttcggg ttagtcatcg
     4081 caaacaacgc ttctatcagt ggtgcagaag ggggatgtca agcagaagtt ggttctgcaa
     4141 gtgcaatggc agcagcagct ttggtttgtg caaaaaatgg gactcctcat caagcagcac
     4201 aagcagtagc cattacattg aaaaatatga tgggactgat ttgcgatccc gttgcaggct
     4261 tagttgaagt cccgtgtgtc aaacgtaatg ctttaggctc ttctcaagct tttatctctg
     4321 ctgatatggc attagcgggt atcgaaagtg tcattccgcc ggatgaagtc gttgctgcaa
     4381 tgtaccaagt cggacgtcaa atgccgtcga ttttcaaaga aacagcagaa ggtggcttag
     4441 ctgttacgcc aactgctaaa cgacttgcgg aagagatttt aaataagagg gcgtgataaa
     4501 gctccttaat ttcgagtatt aagagattag ttatcttcat aacaaaaaac gaagagagga
     4561 ctaattgccc ttctcttcgt tttttattaa tccatcaatt tcgtattttt tacttcatcg
     4621 atcgtttttg ttcctgcaag ttgcatcgtg atcgacaatt ctttgttcag gtgttcaaaa
     4681 acagatgtta ctccttctgc cccacctaga ttcaacccat aaatcactgg tcggccaatc
     4741 gcaactaagt cagcaccact tgccagagct ttgaagacat gctctcctcg acggacacca
     4801 gagtcgaaaa tcactggtac acgtttgttg actacttcgg caatttttgg caatacttca
     4861 aaggacgcag gacctccatc taattgacgt ccaccatgat tggataccca gataccatct
     4921 gcccctgcag agatagcgat tactgcatct tctggcgatt gtattccttt tacgataact
     4981 ggaagatggg tgatttcttt gattgttttg atgtcatctg gtgtcaagcc ttgttttgca
     5041 gctgcataaa tttctgcgat tccttttcct tcgccattac cacttgcact ttgttcgcta
     5101 tacgcagcta agttaggcat tggtaatgga aattggaatt gattgatgac gtcttcttca
     5161 cggtaaccac cgagcgttga atctgccgtt aaaatgatag ctttagcccc tgcttctact
     5221 gctttgttca aaatgaactc attgaatctg tcgtctttac tcatatataa ttggaaaaac
     5281 tgcggtgcgc ctggcgctgc atcggctgcg tctttgattg tcgtatttgc ataagtgctg
     5341 atggaaaaga ttgatccagc agcagctact cctttagctg tatcagcttc ccctttttca
     5401 tgtgccaatc cttgggccgc tgatggcgct tggataatcg gtgtatctaa ttcaattccg
     5461 aaaacagatg tatgcaaatc ggctgagtcg attccgcgta atatccgtgg catgattttc
     5521 tttgtattaa aagaagtcgt attttctttc atcgtccact catcttcaga accaccgcga
     5581 atatagccaa aagctcctgc ttccatgcgc tcttttacac gaccttctag ttaggcaatg
     5641 tttacaatat cgattggatg ttcttcatta cttgtatgat aagtcatagt agatccttct
     5701 ttcatcttct tgattacata taaatcatac taaacttaca aaaagtaagc aaacatttac
     5761 tctcagatac ttttatcgct attctttcca aataaatcga cttacaagct gatctacttc
     5821 ttggttttca tgaagcatac tatgagaggc attttcacct gtcactactt tttcctctat
     5881 ttcatttccg cgttgcttca acaacgcata gaccgctaat gcactgttta acggtactgt
     5941 tccatcactc aagtctgttt catctagttg accagcaatc aagaaaaatc gagtggttat
     6001 aggaatatta ccgatcattt gctggtaatc ttgatagcgg ttagaaacga cagcaggccc
     6061 ctttgctaac tcatctgtca gagactgcgc actatcatcg acaaaatcat taaaaggcgc
     6121 gccaattgct gttaatgtct gaacttgtgg caatgacgta tcctgtccat acgttcctaa
     6181 atagcgtagt gcactaactc ctcccatgga atgccccact atgttgactt tatctatgcc
     6241 gtagttttct ttcagataca aaagacaagt tttgatccat tctgcttgat tccactcgtt
     6301 atttttatta tcttcaaaca atacttggac catcggattg tctgcttttc cagacaaatc
     6361 tcctgttgct tgtatttgtc catctgttcc tactgttaaa accaattctt ttactgcttg
     6421 ctgatttttt tccaaacgcc gcaacatccc tttaaaggaa ttttctgtcc cgctgtagcc
     6481 gtggaaaaaa agtgttggaa tagtggtttt ttctatcact ttactggatt ctgccgtttt
     6541 ttctttatca gaaacttcag tagtatttat cttcgtcgtc tggcaaccac ttaacgcaaa
     6601 caaactgata aggactaaag ctgctgcagt tgcacgtttg cataaatttt tgattggttc
     6661 tgtcatacac ctcttccttt ccatatagaa aaaagaaaga cttgaaaggc caatcatatg
     6721 atcgacattt caagtctctt tattaatcga ttttatctac ttgatcgaaa tcaagttctg
     6781 tactggtttc acgtccaaac atatcgatgt tgactttcaa tttttctttt tctccgtcaa
     6841 cttctgttac ttgtccttct aatccagcaa atgcgccttc gatgattttc acgacttcgc
     6901 caacttctac ttctagatca ttttgtcgca ggctcatacc gatagaacgc aagatatgat
     6961 tgacttcttc tggaagtaat ggtgctggtt tgcttccggc tccgtgtgaa ccaacgaatc
     7021 ctgtcacacc aggtgtgttg cggacaacat accatgaagc gtctgtcatg atcatttcaa
     7081 caagtacgta tcctgggaat gttttatgaa cgatttcttt ttctttccca tttttgactt
     7141 cagtttcttt ttcttcagga acaacgacac ggaaaataaa gtcggccatt ttcatacttt
     7201 gcgcacgaga ttcgatattt gcttttactt tgttttcata tcctgaatat gtatgcaaga
     7261 cataccagtt tttttcaaaa gattccattg aaggctcctc atttcattgt aaaataaaaa
     7321 aacctcagta cacccgaagt ttttctttcg cttcattata gcactaaaag tacgttgtgg
     7381 ctagcgagtt attttaaaat ccagccaaat aagctttgga tggctgtatc cattacgtaa
     7441 aacatcaatc cgaaaataat agaagtttcg atgactacta acgtatcttt acggagttgt
     7501 tttctgcttg gccaagttac ttgtttgatt tcatctttta cgctttttag aaatttcatg
     7561 agacaacctc ccttgctact gtttatttcg tttctttatg taatgtatat tgattacaat
     7621 atttacaaaa cttattgatt tccaaacgtt cgccgctttt tccttcactg actgattttg
     7681 tgtagttgcg agacccacaa acggaacaag cgagtgctgc ttttttcttt gccatttact
     7741 tctgccccct tgctgagaat cagacttata cttcataaca gtatcacgta actttttact
     7801 tgtcaatttg tgaaataaac tatctattgt tttggataaa attatgatat gattaatttg
     7861 aaattaagct aaaatgagac aattggaggt atagcgatgc tattaaaaac acttgtatat
     7921 aagaaaaaag atctgacaac cgttacagag actagtacat tagaagaagc attaaaaatt
     7981 ttagaaactt ccggataccg ttgtgtgccg atcttagatt cttcaggtaa tattttccgt
     8041 ggaaacattt acaaaatgca tatctatcgt cataaagcaa atggcggtga tatgacgctt
     8101 cctgtcactc atcttttgaa aaacgctaca aagttcattt acctggatac ttctttcttc
     8161 aaagtattct ttacgatcaa agagcttcct tatattgctg tccttgacag tgaaaatcat
     8221 ttttatggta ttttgacaca tagtacgttg cttaatatgc tctctcaatc ttggagcgta
     8281 gaacaaggaa gctatgtact gacaattgct tccacaggaa agcaaggaga tttggctgct
     8341 attgcaaaaa tcatttcaaa acacagcagc attgccagct gtattactct tgatatcgaa
     8401 aaagacgaat acattcgacg tacattgatc acgctaccgg ctaacactga agcgaaagta
     8461 tgtgaaacga tcgtcaacca cttagagaaa aagaattata aagtggtaga aattgaaaat
     8521 ttgcaaaacc aagaagcata atcaaaaagc gaaaaaagaa aacagctaat ctgctttctt
     8581 ttttcgcttt ttataattag acacgctttt caaaatcaag cgcgcgatct aaataataca
     8641 tcaatggtgc tgaaacagcc atgacgataa actcacttaa tgcaagcgtg ccataggttg
     8701 tccaaaatgc ttggccacct gttggaacta acatataagc aatcagtccc atgctgatgg
     8761 taaagaagat cgtgttcaat actaaacgca tctttacatt tttcactttg ttttgcaata
     8821 aagctgtcag gcctaaagaa agcaatgttt ggccgccacc aaaaaccaca tccatgatac
     8881 caaaaccaaa gaaggcatta tagacgatga ccccgcctaa aactccccaa agcaatttac
     8941 ggttaaagac aactaaatga ttcaaacttt ctgaaatacg aaactggatc ggccctgagg
     9001 atacaggcgc aactaaaata gttaacgcta gataaagagc catgatgatg ccatttacaa
     9061 cgattacgga taaccgttgt ctgcttttcg ttttttgcat tttctctcct cttttctcct
     9121 agttttttta acgcgggatg gttgatgaac cgcggttatg tgaaacaaac aataattata
     9181 acagactttt catttatttc aatccgtctt atctgctttt aaaaaagcaa aagcttaaac
     9241 aatgtaaacg tttgctaaaa atcgtgggat atggtataaa gtaagtgtaa ggggtcataa
     9301 gaattcccag taaagcagac gaagtaggat ctgcaagaac agaattgttg tgaaacttaa
     9361 ataaataaaa aaggatgtgg gaatttgagc aacctcttac tagagaacgg tctagcaagc
     9421 agctgaagat ctaagtggac atgccgaacc ttggaacttc tccttacgta agtaatggat
     9481 catgcgcttg ctaggctttt ttgtgttttt cggtcttcgg acctttttca ttttcctaga
     9541 cttctgctat aataaccaag aattaatttt gtaacagatt tttttgaaaa aacaagtttc
     9601 tttcattcaa aaaatagaaa aagcttgcta attattggtt aaccagcaaa caggagtatt
     9661 aaaatatctt gttttattga atagcaggag gaaatcatga aattccatta tcatatattg
     9721 atcaatccat cggcaggcag cggaaatggc tataaagtag cagaaagaat tttaccggta
     9781 ttaaaaaata agcatattga ctatacactt cattatagtg aatacaaagg acacgatgct
     9841 gagattgctg aaactttggc aaaagaaaca ttgattcctt ggatagaaga agaacagaaa
     9901 acaatcgata ctttccccct tttgatcatc gtaggaggtg acggcacctt acatcaggta
     9961 cttgatactt tctatcagat ggaagtagaa ttcccagttg cttatattcc agcaggttct
    10021 ggaaatgatt ttgctcgagg ggcaggcctt ccaaaaaatc ccaaaaaagg acttcagctg
    10081 attttagcag ctcaatcacc agaaaaagtc cacataatgg cttatgaaga aaaaataagt
    10141 gaaaaaaaag gaatagctgt taataatttc ggtataggat tagatgcagc tatcgtccat
    10201 gcgacaaatc attcttccac aaaaaagcga ttgaacaaat acaacttagg atcattttcg
    10261 tatttattct ctattctccg tgctttattc actcaaaaag gatttccaat tctggtagat
    10321 gcaggaggaa aacaattaag tttttcaaac gcatttttat gtacagcaac gaagcatcct
    10381 ttttttggcg gaggaatcgc gattgttcca actgctgatg cgtctaaacc agtcattgat
    10441 tttgtcgtgg tcgaacgaat caatttcttt aaaatcttgt ggctcattct tttacttata
    10501 cagaaaaaac aattgaaatc gaaatatttc catcactaca caacaaacag attacgtatc
    10561 gtttcaacaa caccgcaaca tggtcaagaa gatggagaag aaatggaaaa acgacctttt
    10621 gatatcacaa taaccacaac taaacagttg ttttggtgtt aataaaagag gttttgtcag
    10681 aagctacatc tggaacaccg tttattcgga aaaaaggact gtgacacgac ttttgtcaca
    10741 gtcctttttt tactatgcag ttaattcttt gcccaattgg gttgccatcg atagtttctt
    10801 tctttaccca aaataaattg gatcattgaa aacaccgcag caaaagcaac taagacacaa
    10861 ccattgaacc aaaaagattc tgcaaatgtt tgattgtaac tgagtgtttc actgatcaag
    10921 ttattatttt gaacaaacca atatgttggt gtgaatgccg ctattttgct aaccatttct
    10981 ggcaagtatt ctgctggtac aaacacccca ccgataaagc aactgcctgt gataaaaata
    11041 tctttgatcc caccgctggt ttctgagttt tttaccaatg aagtgataca agccgaaaag
    11101 ctaatgatcg gcaataaaaa caagaaagta tttaaaatat gataccatac cgttatatcc
    11161 cacgaggtgt ggatcactaa cagaaccatt agaagtaaga agccactgaa caacagcagc
    11221 gagtaactaa tcaaactaaa ggttactttg cgattcaagt ttcgtttggt aatgggcgca
    11281 ctctggtttc gtttactgat ctctttgcga ttaaacgcta gattgatgac gctgaaaccg
    11341 ctaaaaatgg atagaaacaa cccataagac aagaaattaa aggttgtgcc cgtcaacagc
    11401 tttttcatcc gctgactata tcccgcactt agctcaactt ctccagtaac cgagagtgcc
    11461 atttgtgtct gttccatagc tgcatcgatt gttttatctt cttcttgaaa cagaatcagt
    11521 gtttgcaaaa aggtgttcac ttgctgtgta acaactgttt tagaaaaggc atctgggcga
    11581 ctctgtatct tgatcgtcgc catctttcct acttcgactt gttcggtaaa gtctgcaggc
    11641 agatgtaaga taatgtttac acgattaaaa taaagagcat catcgatttc tttttgtgag
    11701 gttccatcca atgtaacgac tgtttccttg tcattcaaat atttacgaag gccatcgact
    11761 acaggcgact cttcttcact aatcaaggcg attttcgcat cttgcagaga ggtattttca
    11821 acatcaatat tttgcgcaaa aaagaatgtg gcaacaacag tgataattac acctatcagt
    11881 aaattcgatt tatttgcttt aagcaatgtc caaatcgttt tataaagcgt cataacgcac
    11941 ccctctttcc atccaagcat ttcctaacgc aaaaaggaaa ataaatccga tcaaccatag
    12001 aagattggta taaaaactag ccaaatcagt gtagaaataa agtttataca aactttcgct
    12061 gatcaagttg acgagattta attgaccaac aatcggaata tgaagatcaa tccaatactt
    12121 cacttgttcc gaccccatca taccagcaaa aaagctcatc gcaatcgtca ctgaaatcaa
    12181 gatgctatcc ttttgcacca gattcatttt tggtatcaaa ttaccaacca atgtacctaa
    12241 caaaatcgca cacaacgacc ccagagcggt aacaatcatc aaccattgcc aacgttcacc
    12301 aaagtctact tgatagataa aacgaaagac agctaacata atataaatct ctgtcaaaac
    12361 aatcgtaaag cttgctgcca aattagcgat tgaaactaac gattgctttc taggaatcaa
    12421 ggacaagcga ataccatttg acgattgatt tgcttgctga tcctgtacat tgcggacccc
    12481 ccacatcgtc ccgtagagaa tcgccattcc aatcagagtg aagaaataaa aacttttgaa
    12541 actcatatta cgacctgcat gaacttcttg aaaagatact tcttgttcaa aaacagtcat
    12601 gatgggcgcg agatcaacgt tctccacttc tgccacttta ctcgaaatta taccaatacg
    12661 ctgtaaaaat tgatccatga cgtttttcat caacgtctga ctcattcctg cttgacccac
    12721 atggagcgaa acagatgaac catcaatgag ataaaaacca gccaaatcat ctgcatttag
    12781 cttttcattc gcttctactt ccgatagttc ctgtacttca aataatggtt tatcattgac
    12841 ttcgacttgt tctaagactg tggtaaaatt ttccgaaagc gctgtttgat ccgctttgat
    12901 tacaattcca gtattaatcg tttcaagatt gccaccttga tcaatctcgc caaaggccgc
    12961 cataaacatc aatcctaaaa gaattggaaa ggccaatgtc caaaacaaca tggatttatt
    13021 tcgcagcagt atttttgtgc gatatttata aaaatgccaa aacattattt cccctcctct
    13081 agtctctcaa atccttgcca gtaatctcta aaaagacatc attcaaggac gcttctttgg
    13141 ttgctatttt tccgtaactg atcttttctt cttgtaagat tgtaagaatc ggcaacaata
    13201 ctgtatttag ttcagtagtt gttacttgga gctgaccttt tttataagcg attccatcag
    13261 aagctattgt tcgctgaatc gctgacaaca cattttctgg gagtaatggg atgtcgatcg
    13321 tcaaacgatc cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca
    13381 cctgtccttg atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat
    13441 gtgttgtata gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt
    13501 tattccgact ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct
    13561 tgtgggcaat cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg
    13621 gacgaaacga aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt
    13681 tccttttctc tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac
    13741 ttagttcgtc aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat
    13801 aattttcagg agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca
    13861 aagaaagcat acagttgatg gcagtcgatt tcccactgcc gtttggacct aacagaccta
    13921 agacttctcc ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc
    13981 gtttgactaa tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt
    14041 gatctgacta taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg
    14101 acatttgtca caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc
    14161 tttctactgg ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt
    14221 atctatttat tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca
    14281 aattggattt tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg
    14341 tttctgccag cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt
    14401 ttgttaggtt ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt
    14461 gtcgtaattt tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat
    14521 cttgagaaaa agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg
    14581 gaacaaaata aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa
    14641 gaacggaacc gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg
    14701 attttacaaa ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta
    14761 gaaaatttaa gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac
    14821 ctccatgaga catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct
    14881 tcttctgtta cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc
    14941 ttcttaatgg tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc
    15001 gttgttttac attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc
    15061 accagcccac aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt
    15121 caagaacaaa agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc
    15181 attttaccaa gggagggaca ccaatgataa cagtagtcat tattgatgat gatccatttg
    15241 tgacgaaatc gttacaaacc attttagaaa gcacgaaaga gatccgtgtg ttaggcattg
    15301 gtcattgtgc caaggatgcg ctagtcttat acgaaaccca tcgtcccgat gttttgctga
    15361 cggacatccg catgcctaaa caaacgggca tcgatgctgc caaagaaatt ttggctaaat
    15421 ttcccaaagc catccttttg cttttgacga cattcaaaga tgaagaatac atccgcgaag
    15481 cattttccat cggtgtcaaa gggtatttaa tcaaacagaa cttgcaagcg attattcctt
    15541 ctgtcaaatc cgcttataat ggacaggccg tatttggtaa tgaaatcatc gagactttca
    15601 ctcaaatgat gaagaataaa ccgacgattg atagtcatgc ttatgcttct tttagtgaac
    15661 gtgagcttgc aatcatcaaa gaagtagctg ccgggaaaaa caataaagaa attgcagatg
    15721 ccttgtactt gagtgacggc actgttcgca attacatcag ccaattacta gaaaagctag
    15781 atttacggga tcgcacgcaa ttggctatct atttttatca gcatttgcaa taagtcttat
    15841 ctaacacaaa aaaacctgaa tgatgctcct tcgcatcatt caggtttttt tcacatcttc
    15901 tattcaattg tcccgcaaac catttcctca cgattgaaag aatcaaggat tcgcagggct
    15961 gccaggattc gttggattgt tcggcgtgtt aggcggaaaa ataggcgcct tgtctaacgt
    16021 tttttctagt tttgattctt cacgatcgtc tcgttctaca tcaggacttt cattcaattt
    16081 gaattctttt tccatctcat ctaacttttt aaagtcattg ccagattctt ttgctaattt
    16141 cgctaattta tcttcggttt ctttagccat aaagtaacgc cccccttttg atttcagctt
    16201 agcatatcac actgaaaaaa caaaagaaaa cgctaaccaa cgatcatttt tagctatttc
    16261 tcatctttaa aatcaagcat tttttgccgg tataaaatcg tagggagaac tgttttccat
    16321 accaatcatc ggatctacta actggttatc gatcacttct gctgtaagaa cgaaatcttt
    16381 agaaacagca ggttctacct cttctttctt ttcttctcca acaaatgaag aaaacagatc
    16441 tggttccact gtttccatta cttggttttt cgtttcattc gtcccactgt catcttctac
    16501 aggtagcttt tcttttacaa cccgtggctc ttttgtatag ttcccatctt caaatggatc
    16561 atttccgacc aaagaatctt cataagctgc tagtttggtc cggagagtga tattcatttt
    16621 atcattatcc atgatccttg atttcaacgt agtcatccgg cttgctgatt cattcgctac
    16681 acaagtgaca taggcttgct cttcccctaa caaaatcaac agttccttac tgcgagtcac
    16741 tgccgtgtag agcaaattac gctgaagcat ccgctgatat tgatggacca tgggcaaaat
    16801 aaccatcttg aattcactgc cttgtgattt atggattgag caacaataag aaagagtgat
    16861 tttattccat tcgtttcttt tataagtgac ctcatttgca tcaaactgga taactaattc
    16921 atccacttta tcttcagaat cttttgccaa gatgataccc gtgatttctc ccatatctcc
    16981 attgaataca ttcaattcgg gagtattgac taattggagg actttgtctc ctatccgata
    17041 aacggtgtcg ttccaatgta cttccttttt tccaggtttc cctggattga atatctcctg
    17101 catcatttta tttaacgcat cgatccctgc aggtccccga tacattggtg ctaatacttg
    17161 gatatcttgt gcacggaatc ccttattttt cgctttttcc acaattttag agatcaaggg
    17221 ttcaatttga taagcatcac ttcggaaata ggaacgatct ttttgattct ttgtgaaatc
    17281 ttttggcaga cgtccttctt tgatctcatg ggcaagagga ataatactag aaccatcacc
    17341 ttgccgataa atctgattga gttccatttt gggaatttgt tcgatttcca aaagatcatg
    17401 gagtacttga ccaggaccga cagagggaag ctgatctttg tctcctacta agataacttg
    17461 catattcgtc ggaatcgctt tgaataatgt gttagccagc catgtatcga ccatagacat
    17521 ctcatcaaca atcaaaagtc cgccttctaa ttctttcgta ctgactgaag gtgttttttc
    17581 ccgtccattc agccccagaa gacgatggat cgtactggca ggcagacctg tcgtttcgtt
    17641 cattcgttta gctgctcggc cagttggtgc tgcaagtaga ataggaaaaa tttcttgggt
    17701 atattttgaa ggatccaatt ccagtccgtt caactctgca aaaagattga cgattccgtt
    17761 gatcacagta gttttccccg tacctggtcc tcctgttaaa ataaatagag gggaacggat
    17821 tgcttcagcg atagcttgtt cttgcgaagg tccatactgt atgccgaata ttttttcgat
    17881 acgccgaata ttcttctgca cttctttttc aggataatgg atttcttttt tacgactgag
    17941 aaggcgctgg atcgaattac cgatccccca ttcagaaaag tacaaactgt tttcgtaaag
    18001 attagtctct tcttgttgta tctttccttc ttcaacaagt tggataatta cagtagcaac
    18061 ctgatcaggc tggatttcaa ttgggcgact tgtttctaaa atacggatcg tttgctgaag
    18121 caatgtctcc gccggtacgt atgtatctcc tgtttgaact gcatgctgaa aaatctgatg
    18181 caagatagct gctcgaattc ttctcggtga atctgctgca ataccgattt gttcggctag
    18241 attatctgca cgtttgaacc cgattccttc tatgtcttcg actagctggt acggattttc
    18301 ttcgatgata tccaaagact cgttcttata ggcctggtag atactgaaag ctaactgact
    18361 accgaacccg taacgattca gtcccatgat cacttgatcc atcccgtggt ttagccgaat
    18421 cgtctcagca agcatttctc tttttggctt cgtcaaaccg gagatttgtt ttaataccga
    18481 aggatcagca atgatttttt cgatagcatc ttctcccagc aattctacaa ttttttctgc
    18541 tgttttcttc ccgattcctg ggaatttttc gctggaaaga tacgctacga tcccatttgc
    18601 agttgttggc ttttcttgct catatctttc gagtttaagc tgccttccgt atttaggatg
    18661 atccactaat tctccataaa aacgataagt ctcattttct tgaatatcgc cgaaacttcc
    18721 ggtgactaca atctcttttt cacggtaatc agctgtggtt tcactgatgt gtaccaaaag
    18781 cactttgtaa aaattactag gattttgaaa aaaaatcgcg gcaactgtac caacgaaata
    18841 aggcatttct tctggctgca tcggaagccg cctccttctt ctatagtaaa ggttctggtg
    18901 ctgtttcagt tcctaaaaaa tctgtttggt tccatacttt cagttcatat ggcaataatc
    18961 tgcaaggctc ttcagtctct aaaatcgtca aactactatt gaacaatcca cccatttcac
    19021 gcaactcact tagctctttt cctgtcatcc attgaatggc tgcagttaaa gaagctccat
    19081 gtcctacgaa taaaagtggg ccatctttat gtcgggcaac cgcatctgct acaacagttt
    19141 cgatacgtgt gattgccgat tcaatcgttt ctccgtgaaa aggagaagga tcatacagat
    19201 ctaaatgatt acgcaaattc ggcagattat ccggatattt ctggtacatt tcatcgatcg
    19261 actgaccttc taaagtaccc agccctagtt ctcttagctt atctgtataa aaaatctcta
    19321 ccggacgagc aagctctttg ttgatacctt ctgctgtatc acgtgctcgc tttgctgtac
    19381 tggaataaat tgctttaaaa ggaacatatt ttaccttttt cccaaatgct ttgatttctt
    19441 cgtaactttg tggcaaaagc ggagaatcgc cattgctccc ttgaaaacgt ctttcctgat
    19501 tccattgtgt cttcccatgt cgggtaaaat atagttccat acgcagcaaa ctcctgtctt
    19561 tcttttattt tactctataa taaactgtct aatccagctt gtcattttca tagtgatgcg
    19621 tcaattctaa attagggaat ccttgctggc gaagtgcttc atacactatc attgcagctg
    19681 tattcgataa attcaatgag cggacatgct catcattcat agggatgcgt aaacactttt
    19741 cttcgttttc acgcataaat gtttctggca aaccggttgt ttcttttcca aataaaaaga
    19801 agtgctcttc tccatcagca aaattttcgt cactatatac tttatgggca aattttgaga
    19861 tcaaatgtag tttacgatta cctaaaaatg ttaaaaaagc ttctaaatta ctgtggtaag
    19921 taatatttac atcattccaa taatccaacc ctgcccgttt tagatgtttg tcatcagttg
    19981 aaaaacctag tggctcgatt aaatgaagcg gagtattcgt ggctgcacac gtacgggcaa
    20041 tatttccagt gtttgcaggg attaatggtt caaataaaac gatatgattc atcaggatca
    20101 ttcctctcat atttatgttc gtttctattt tctgacaatt tgatttttgc ataaaaaaaa
    20161 acgtatcaac tcggtgtcag tcactgcgag tcgttacgtt agtgaagctc ttctcactaa
    20221 cttttatcag aacaggaacc tgataaaaac caatgaaaaa acaacttgtt gctaatataa
    20281 caccctcaac ttttttttgc aacctctact cattaaaaac tgctagaaaa acgttttctc
    20341 ttagttcctt ttaataattc tatgtcaatt gtaatcttct tcaaaggatt cttatataag
    20401 tctgctttga ttcgctcttc tacctgccat tctaaaggtg acatggccaa aagatctttt
    20461 tgattttcct ctggtatctc aaattcatag gttacacgct gacgttcagt taatttcatt
    20521 gcttcagcaa acttggctac tacacgttca ttggaataac tctgtttttc aggttgattc
    20581 ggataaaaag cctgacgtag ttcctttaaa taattttctt gtggcaccac cttcaacact
    20641 tctccatctt ctgagagtac acgttggaat tcttggtaat gagaaggcga aaagatattt
    20701 aaaaccgcgt tgaatgtttg atcagcaaac ggcaaattgg tcaaatcagc tacacaccaa
    20761 aatgcttcgg cagattgttc tgtagctgcg tagactcctt cttttgaaat atcaaatcca
    20821 acagcatttt gaatcttttt caattttgaa agttcattca agaagctacc ttctccgcag
    20881 ccaacatcta atagcgagac ttcttcaggt aaatgctgcg caatagtctc aataacgggc
    20941 tgatacatgc cagattggat cattctacgc cttggaacga acattccttg atcgtattct
    21001 gttttaaccg catgggataa aaaataaagt gttccttttt tggaaaggtc aaaacggtga
    21061 ttatttttgc atatcaagcc attatcagac ttttttactt cttcttggca aaccggacag
    21121 cggaacagct gatgattttc tgccaaaaac tggcgtcctc ggtcaatttt ttttaacata
    21181 gttccccctc atttctgttt tatcttaccg ttagatgctc actttggcaa ctgctaaaaa
    21241 tatgatacaa tgcctagaag aaagtgaaag ataaaggaga agattagatg atcaaagaat
    21301 tttgcgctga aaactacaca tctattcctc tagcaattgc tagaggagcc gatcggatcg
    21361 aattgtgtga caacttagcg gtcggtggca caaccccgag tactggtgtg atcgaagaaa
    21421 cgttaagtta tgctggggaa aaagatatcc ccgtaatgac gattattcgt cctagagggg
    21481 gcgacttcgt ctataacgat atcgaattga aaatcatgca tactgatttg attgaagcaa
    21541 agaaattagg gacggatggc gtagtatttg gttgtcttac tccttcaggc tggctagacg
    21601 aagaggcctt agaactattg atcgaaacag cagaagggtt gcagatcacc ttccatatgg
    21661 cttttgacag tattcctgaa gatcggcaat tcgaagcaat cgattggctg gtagaacatg
    21721 gtgttgaccg tatcttgact catggcggtc ctgctgacaa aactattgta gaaaatttcc
    21781 cacggcttaa agaattgatt gcctatacag atggccgctt aatcattttg cctggtggtg
    21841 gtgtcactgc agagaatgca gcacatgtag ctaaagaatt gaacgtaagc gaagtacacg
    21901 gaaccaaaat cgtggatctt cagttataat cttataaaca aaaaaacatt tgcaacaggc
    21961 ataatgctta ttgcaaatgt ttttgttttt ttacataaaa tagcccataa ataaagaagc
    22021 aatattgaaa taaatcagta cactcgtcaa atcgctcaaa gtcgtgataa agggaccgct
    22081 tgctactgct ggatcaaatc ctagacgatc cattagcatc gggattaaac tacctgcaag
    22141 attcgccact gtgattgcaa atagcatagc catccctatg acaaacccca agataaaatt
    22201 atgtttccaa aaacctacga ttagaaaaat cgtgatcccc gtaacagctc ctgtgacaac
    22261 gccggtcaag acttcagcca aaacaagtcg aacaaatcca tgatctttct catcagaaat
    22321 cgcaagtcga cgaacagcca cagctagcga ctgggttcca gcattaccgg cagttcctgt
    22381 aatcagcgaa atgaagactg ctaaaatact cgcttcactt accaagtctt catagtgact
    22441 gattaaagaa gcggtcgaca tacctaaaaa caacagagtg atcagccaag gcaaacgtcg
    22501 agacgctgcc tttactggat tttcattaat ttcttccaca ttgaccccgg caagtcctga
    22561 ataatcgcta gcagcttcat catcaataac gtcgatgata tcatcgactg tgacgattcc
    22621 taacaaatga tcatcataat cggtaacagg aagagctaag aagtcataat ctctaaatgt
    22681 ttgtgccaca tcttcttggt cgtcgcctac atgaactgaa agcactcgtt cgctcatcag
    22741 atcagaaatc attgcgtcgt catcattcac gattaggtcg cgcagagaaa taacccctac
    22801 tagctgacct tcttgattaa cgacatagat gtaatagatt gtttcagcta catctgcttc
    22861 gttcttcagc acgtacatcg cagagcgaac agtttgattg gctacgatcg aaacaaattc
    22921 agttgtcatg atcgcgccag ccgtttcatc ctcgtaatgg agaagttcct tgatttcact
    22981 ggcatcgtct gccgacatca aacttaaata tttagcaatt tgctttttat ccagcgtatt
    23041 aagcaagtca acagcattat ctgtatacat ctcagaaagc atatcagctg catagctagg
    23101 acgcatttca tctaggtatt ctttcatatg ttcgttgtct tcttcaatca catcgaacat
    23161 atccgctaat tctttaggcg acagatagga atagatcagc tgcctgttct cttctgtaag
    23221 agattggtag aattgcccct gctcatagat atgcatctct aaaaaactgt ttctgaatgc
    23281 ctgaatatta ttttccctta aatcttgagt caactgggca aatctttcct ctaattcttg
    23341 attttcatcc aaaccaatct cacctcgcct tattcttcaa accgtctcac aactgcctcc
    23401 atgtctgcgg ctaaaggctg cttgaactcc agcaactctt ctgtaaatgg atggtaaaaa
    23461 cgcagatcat aacaatgaag ggcttgtctt tttattccca gatccattcg cccgccatac
    23521 atatcatcac cgagcagggc acagccaatt gcggaaaaat gaactcttat ttgatgtgtc
    23581 cttcctgtat gcagctggat atcaactaac gcgatttctg ttagtcgctt ctctatccag
    23641 tactcagtct gtgccgaacg tccttcatca gagaccattc gcttcaatag cgaagaataa
    23701 tctcgggcga tcggcaaatc gatatgtcca tgtgccgata aatctgaaag ccttccactg
    23761 actaaggcct gatatttttt cacaaacttc ttttcacgaa gttgcatatc taattttgcg
    23821 tgcgcaaatc catgctttgc aaaaagcatc aagccagatg tatcccgatc tagtcgcgtc
    23881 accacatgga ttacttggtt ttcgtaacct tgttttttat aataagactt tacacgatta
    23941 gccattgtcc catttggatg gtattgggca ggaatagacg aaacgcctgc tggtttattt
    24001 actaccagaa cgtgttcatc ttcatacacg atatcgatcg gtgtttcatc caaaagtacg
    24061 gtttcatgtt ctccttcagc aggaatcaca atggttacct tatcatcctt agctagagaa
    24121 aacaagacat tttctacttg atcattaact ttgatttgac cgccttgaaa ttttattttc
    24181 gctaacagac cctttgagat ccctttttct tttaaaaaat attttacttg ctgagaatga
    24241 tctttctcat aatcccatgt aaactgcata tcaatcctca ctaataaagg catctttcac
    24301 acgatgccaa aaatgcatat gtcgataaga agcaaaatgg atccgttctt ctgctattcg
    24361 ataataaatt gagcggatat ttgcttttgc gacattcaat tgatcaaccg ttacaagata
    24421 atcttctgta ttttccaatt ttatttctag ccattcatca tgggcaatca cgatcggaga
    24481 acccaaagta cgaaacactc gattattcag tgaagcaatc tctgccaact gaaaggcatt
    24541 gatactagga tggatcacag caccgccgac acttttatta tatgctgtcg aaccagtagg
    24601 cgtagaaatc gtcaatccat ctcctctaaa actttcaaac agctcgtttt tgatgtatac
    24661 atcggctacc atcgtacgat tggcccgttt aatcgttgat tcattcagtg ctaaaaaatg
    24721 ctggtccggt gtttcatcca aataactgat ccgaacatcc agcaagggat aactaacact
    24781 ttgttcccgg ttcgttcgta agctgtctac tagttcttcc agctcgtaat cccgccaatc
    24841 cgtgtaaaac cctaaatgac ctgtatgcac gcccaagaaa cgcacatcat tcagacaatg
    24901 actgtagtga tggaacgccg acaataatgt gccgtctccg ccgactgaga tgaccaattc
    24961 tggctcatga tcatctcgtt gaatccctgc ttgttctaaa agtgcgttca aacgttctgt
    25021 cacttctata gtctttgctt ctttattatg aacgatcgct actttcatcc cctgcctcct
    25081 ctctttcgct ttctttcttc aaaagcgttc gatagtagac atcatctgat tttcctcttc
    25141 catgggagaa taaccgctgt gcttcttgga tttcttcacg gatactggac atctcttcat
    25201 ccaactgata ggcagcttct gccgcacgct gaagtcgttc actcatttct tctggaaaaa
    25261 cgccttgata tttataattc agcgaatgct caatcgttgc ccagaaattc atcgcaagag
    25321 tccgaatctg gatttccgcc aaaattttct tctcaccgct gatcagctgt acaggatact
    25381 cgatcaccag atgataagaa cggtagccac ttgctttttt atttgtgatg taatctcgtt
    25441 cttggagaac tttcatgtct tttcttttac ggatgatctc aacaacttga tggatatctt
    25501 ctacaaactg acacatgatc cttaacccgg caatatcaga catttcttct tctaaacgtc
    25561 ccattggaat atgccgtaat tgtgattttg ttacaatact atcgactggc ttgactctcc
    25621 cagtgacaaa ttcaatcggg acatgtcgat tttgttcacg gaactgtttg cggatccccc
    25681 gcagtttcac tttcaattct gatacagttt gttcatatgg tgctaaaaat gcttcccaat
    25741 ctcgttccat agctgatacc tctacttcat taacattcat tgtttattct accatatatg
    25801 caaagttttt tcgtactctt gatttatgca ttgaccaaaa aaagaaaatt aggcaaaata
    25861 gacaaaaagg agtcgatcaa atgacacaaa tgcttgaaat cgaatataaa tctatgctga
    25921 caaagcaaga gtacgagata ttcatcgctc actatcaatt aacagataaa gatttccgtg
    25981 tacagactaa tatttatttc gataccgttg atgaacagct gaagaaacaa aattgtggcc
    26041 tacggatccg tttcgttggt catcaagctg aatatacatt aaaaacacct gctgaaaaag
    26101 ggcgtctcga aacaacggat acctttataa cagaagaagc agaagcattt atcttcgaaa
    26161 aacgtctgcc tcgttcaggc gcagtttttc aaaaactttc tgatttgaat atcgatcctg
    26221 cttcattaat ccaaaccgga aagcttacaa caaaacgagc tgaattccct attgaagaag
    26281 gacttttagc tattgatgaa agctggggag acaatctaca cgattttgaa ctggaactag
    26341 aggtagggga tgcatctgtg ggaaaaattg cttttataaa ttttttaaaa cgtttttctt
    26401 taccttatcg tcctgctaag aacaagatcc aacgtatgct tgaagctaaa aattagaaac
    26461 ctttcatttt cacaaggcac ttgagttcat ttttttactt ttttctgtta agttatagga
    26521 tgtgaaagaa tctagtcact agttgtatag aatctatttt gggggagatt catatgatcg
    26581 aaatctattt atttgttaat cctttagggg aatactgttt cgaaatggaa caacagttat
    26641 tacaatttat cgaagaagag tacgggacac catcaaaaga aaagatgcaa tttcgctttc
    26701 ttcctttggt caatttgcag acaattgggg atgtcatgca acaaaaagga atttctcaga
    26761 acaatcttga ggaacgtaat cacttattct caatgactta ttctgcagca ttggactgca
    26821 aagcggcgca gtttcaagga aagaaaaaag gccgccgttt tttgatcaaa ttgcaagaag
    26881 cagtcggctg taatgacata ccatattctc aagaacttgc tgaatccata tttgaagaaa
    26941 tcggcggcga tttgaatatg tttaaagaag atcgtcagtc tgacttcgtt aaagaagtat
    27001 tcttttctga tcaaaaaatt gctcgggaaa tgggcatcac aaaacaccct tctgcagtcg
    27061 tttacaatta tgcctgcgac cgtgattttg gtgttttagt agaagtaaaa ggtacaagta
    27121 gcctttggag aatcacagag ctgtgtaaaa caacagataa ctcatatcaa gtttttaatg
    27181 gtgaacattt acatacaaat gattctcagt tccattcaag aactagccat ttacacttgc
    27241 tttctaatta aaaaaggctc tttgtcaaat gatgttggta aagagaaatc ttagaataat
    27301 ttggacagaa cagaaagaag aaaacctctt tctgttctgt ttatttggtt aaaaatgttt
    27361 tgccatgacg aatcataatt cgtagtttga aattgtaaaa gttgcgaaag ccgtacgcat
    27421 tgcgttttaa tactttgatg tggttgttta agcattccaa tggtccgttt gaatagctgt
    27481 agtgaagcgc attttggatc tgtggcagaa actttttaaa cgttcgtaaa gtggttgtat
    27541 aggtttctgg cagaccgcta atgtctctag ttaaacaatc ctcaaatagt gactgatctc
    27601 ttttctttac cgcataaaga atgtcttggt ataactgata gccttggcgt aagttgggac
    27661 aagcagctaa cagtcgatcg gcaatctcac tttcagtcaa ataatcttta aaactggcac
    27721 gccaaacccg tttttcatag tttaactttg cttcttcttt ttgtaggagt ttccaatagc
    27781 gcttcaattt tttccctaac cctcgttgag agtgggaatt tcctttaagt cgtaccgtgg
    27841 tttctttcac tcgatgattc aagaaagttc tgccaatatg ttggacgatg tggaaacgat
    27901 cgatgatcaa ctgagcattc gggaaaagct ttttcaccaa cgaaacataa ggcgcataca
    27961 tatcaataac aatcaattga acggcttcac gagtcgctaa tgaaaaccga gaaaaatagc
    28021 gttctaaata aggcaagcgt cgattttcga caatatccaa taatgcatgg ctttgtccgt
    28081 ccatcatgat aaaactcatt gcactggcaa cacttcgaac cgatt
//
LOCUS       DAXWLF010000018        27157 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXWLF010000018 DAXWLF010000000
VERSION     DAXWLF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.018"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(172..1590)
                     /locus_tag="ACGE4U_000591"
     CDS             complement(172..1590)
                     /locus_tag="ACGE4U_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8392778.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIDGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(1830..3398)
                     /locus_tag="ACGE4U_000592"
     CDS             complement(1830..3398)
                     /locus_tag="ACGE4U_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFF8392779.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(3402..3812)
                     /locus_tag="ACGE4U_000593"
     CDS             complement(3402..3812)
                     /locus_tag="ACGE4U_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFF8392780.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(3809..4732)
                     /locus_tag="ACGE4U_000594"
     CDS             complement(3809..4732)
                     /locus_tag="ACGE4U_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFF8392781.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(4729..5607)
                     /locus_tag="ACGE4U_000595"
     CDS             complement(4729..5607)
                     /locus_tag="ACGE4U_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFF8392782.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(5594..6931)
                     /locus_tag="ACGE4U_000596"
     CDS             complement(5594..6931)
                     /locus_tag="ACGE4U_000596"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFF8392783.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(6934..8037)
                     /locus_tag="ACGE4U_000597"
     CDS             complement(6934..8037)
                     /locus_tag="ACGE4U_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8392784.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(8111..9619)
                     /locus_tag="ACGE4U_000598"
     CDS             complement(8111..9619)
                     /locus_tag="ACGE4U_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8392785.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(9585..10277)
                     /locus_tag="ACGE4U_000599"
     CDS             complement(9585..10277)
                     /locus_tag="ACGE4U_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFF8392786.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(10299..11273)
                     /locus_tag="ACGE4U_000600"
     CDS             complement(10299..11273)
                     /locus_tag="ACGE4U_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8392787.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            11573..12445
                     /locus_tag="ACGE4U_000601"
     CDS             11573..12445
                     /locus_tag="ACGE4U_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8392788.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(12903..15101)
                     /locus_tag="ACGE4U_000602"
     CDS             complement(12903..15101)
                     /locus_tag="ACGE4U_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8392789.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            15333..16325
                     /locus_tag="ACGE4U_000603"
     CDS             15333..16325
                     /locus_tag="ACGE4U_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8392790.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            16385..17806
                     /locus_tag="ACGE4U_000604"
     CDS             16385..17806
                     /locus_tag="ACGE4U_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFF8392791.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            17799..19298
                     /locus_tag="ACGE4U_000605"
     CDS             17799..19298
                     /locus_tag="ACGE4U_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFF8392792.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            19337..20731
                     /gene="uxaC"
                     /locus_tag="ACGE4U_000606"
     CDS             19337..20731
                     /gene="uxaC"
                     /locus_tag="ACGE4U_000606"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFF8392793.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            20890..21573
                     /locus_tag="ACGE4U_000607"
     CDS             20890..21573
                     /locus_tag="ACGE4U_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFF8392794.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            21575..22411
                     /locus_tag="ACGE4U_000608"
     CDS             21575..22411
                     /locus_tag="ACGE4U_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFF8392795.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            22422..24176
                     /locus_tag="ACGE4U_000609"
     CDS             22422..24176
                     /locus_tag="ACGE4U_000609"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8392796.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(24452..24793)
                     /locus_tag="ACGE4U_000610"
     CDS             complement(24452..24793)
                     /locus_tag="ACGE4U_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8392797.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(24861..27032)
                     /locus_tag="ACGE4U_000611"
     CDS             complement(24861..27032)
                     /locus_tag="ACGE4U_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFF8392798.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
BASE COUNT         8110 a         5574 c         4816 g         8657 t
ORIGIN      
        1 aaaaaagggc tccgactaaa taaatgcctt ttggaataaa tacaggtaag tgttctttcg
       61 caccttgatc gattgctttt tgcagtatta ttgtatcatc ttgttgagtg tcgtttgttt
      121 taaagttcaa agcattgata atcccttttt ttgtccgcaa tacgtttctc cctatctttc
      181 attgattttt ttcaattctt caccaataaa tacagttttt gaacgattca gtgggtaacc
      241 tactagcaaa atgattgcgg atacaaataa gcagaatgct ggtaacaagt ttgccatcgt
      301 ataaatcctt tcttgtacta aaactgattg gatggctcca cctgttgtag acgattggta
      361 accaatgagt gccagcataa atccgcctac cgctcctgca catgcttgtc ctacttttct
      421 cgcaaaagaa tttactccat atactgttcc atcttctctt aagcctgtct tatattgatg
      481 ataatcaatc acgtcagtaa tgaatgccca gaccatcaaa ttgaataaac cagatcctaa
      541 agtagcgata aataaaagaa tcagatatac ccatgcattt gttatatgaa ggaaatacat
      601 cagcaagtac atacatgaag aagcaagtaa cgcaaatacg cttgcttcct ttttaccaaa
      661 taattttgtc agctttgtag caaatggtgc taaaactact actgttccgt aagtaaataa
      721 aagagcaaca gacattgctt gtttattatg gaagtaatca ttgaataaat aagtcatatt
      781 cgtcccagct aaaatctgat tgatcacaat gaaaatgtca acaatgacta aaataatcag
      841 tgctcgatta gctaacaaac cttttaccaa ctgagaagca gatacttttt cgtttttgac
      901 aactcttact ctttcagtag tcaatttaca agttaacata tatgcgacaa atgctattgc
      961 agcacatccg attgaaatcc aaaagaaatg attccctgaa acgacttgtt gtccactctt
     1021 gttatgggta tacattaaaa tcggaatgaa gaatcctgta gttgatccgt caatcgcaga
     1081 accaatgctt cggaacgtgg acaacgaggt gcggtcatta ggatttgagc taattgctgc
     1141 tgccattgct ccatacggga tattgattgt tgagagaaaa atcccataaa caatgtatga
     1201 aacaaaaata taagcaatct tcaccgtcat tggaagatgt tggacgaatg gcaaaaacaa
     1261 aatgacagtc actaagcaaa atggatactt catttttctg atccaaggaa taaatcggcc
     1321 agtttctgtt aactctgaga catcacataa tcttccaacc atcacatcag caaacgcatc
     1381 caaaattctc gaaatcaaga ataacgtacc tacgattgcc cctgaaacgc caagaacatt
     1441 cgtataataa atcatcaaga aactattgac taaacctaaa ataaaacagt tacctagatc
     1501 accgaacatg tagcctatct tatcggaaat cccaaattcc tttttttctt tttttattgt
     1561 atttgctgta acagcatttt gtgaaagcat ttgaattcct cctacctctt ctagtaaacg
     1621 ttttcaaaat tataactctt tttttcttgt ttttatattc acaatgttgg ctttttattg
     1681 cattatcttg acagataaaa aacaaaaaat aatctaaaaa cattgatgca taagattgat
     1741 aacttttttt acataacaat atatacaaac aaaaaaattc actaactagt gaaaataagt
     1801 tagtgaacct catttattaa agaattaaat tacagatgat cttttccatc atacttgaaa
     1861 aactcaaaat cagcataact tcctcttttt ttatttagat catgcactgc tatcccgata
     1921 aaatttccag taaaccctcc tgagagtata cacatgtttt tttgcggacc tactttcttc
     1981 cattcttcag ttgatacgtc tttcaaatga aactgtgctg tctctttctt tacagtgact
     2041 ttcaaaaaag tcttcttttc tgtgagtgga atcgtccaat cttctatttg tgctactccg
     2101 ttttctgatt ctatcatacg taggcatttt cccttgtcct cattccaagt aacataacaa
     2161 taaatataat tggattcatt taagtacagt accagtccag ccatctgatt aaaattatta
     2221 ggcatgtatt caaatgctgt ctccgcatca aaagtaaaat ccgtttgccg aatagctaac
     2281 aaatgatgtt cgaaggtaga ttgaagagat tcaccggaaa tcagacgtaa aaaacctggt
     2341 ctacattttg tatcacacca atcagcattt ggcaaaatgc gtctggcatt ccagttcttc
     2401 tttagctctc cttcaaatgt atccataaac tgatgatctt tttcttgttg tacctcttca
     2461 tttgtctcaa tcattatttc ttccatcgga gttgtctggc tatctttaag tcttaaccaa
     2521 tcgtcggacg tccaatacac ttcttgaata gcggtttctc ttcccaaaat agccgcaccg
     2581 tttaatggtc tggtacaaag atatgtcatg taccatcttc ctgacggggt ttgtactaag
     2641 ctcccatgtc ctgtacattg caatgtagaa gcaggtttgt cactagctgt cagcatagga
     2701 aaaccaggat caagctcgta aggaccagta atattctttg ctcggcaaac tgtaactgaa
     2761 tgtcctcttc ctgtaccgcc ttctgccgtc atcaaataat aatacccgtt acgaaaatac
     2821 aaatgtggtg cctccgtttt cgctaattct gtacctgcaa atattttata gatgggacca
     2881 tcaagcttct tcttctctgc atcatattct tgtaatacga taccgctgga tttattcggt
     2941 gtgtccagac gataatccca tagttcattg acaagccatt tttttcccgt ttcaagatca
     3001 tggaacaaag aaggatcaaa gccgttacta tttaaataaa ttggttcgga ccaaggacct
     3061 gtgatctcag atgcagtgat caaataattg tggacatctt taaacggacg gcctgcactt
     3121 ttgacatctg tgtaaacaag ataaaacaat ccatcagcat aactgatctg tggcgcccat
     3181 atacttccat ttgtaggatt tccttgcaaa gatacttgag tggtcaaaat atccgttgta
     3241 tgtgaccagt tgactaaatt tttcgactca tatactcgga ttcctggaag ccattcaaaa
     3301 gaggagacaa taatgtaata ccgctctttc actcttaata tattaggatc tggattgaat
     3361 cctcttaaaa taggattttt cactttgata gtctgcatga ttcaggctcc ttttctcatt
     3421 cttactcgat ataaactgaa tgccccagct gcgaaataaa aaaatgcaaa aggaaacgca
     3481 ataaactgag tgccaatcaa caatatgaat cctgttataa aatacaaacc agtggcagtt
     3541 gttaagtatt ttcctttagc agtcaaaaac aatgctactg ctaaaaaagc aagtgaaata
     3601 aacaacacaa cggcaaacag accagtcatg tcctgtaata atccaaatgt attttctaaa
     3661 gacaaagttg aatgatctac agcaagcgac tgataacttt ttttaaactc tgacgcagac
     3721 atattactta aagtaactgc taacccgcca aagaaaatca aagatagact gccaccgacc
     3781 attcctagca atagttccca ttttcttttc atttctgaac cccctgcttt tctaaatgta
     3841 tccattcagg tcttttgatc gatgtttcat aattgttatt acgttcagag aatttgacag
     3901 tctgttcatt tttcttgtca tcccaattat cccaaccagt tgggtgaaca tgcccaccta
     3961 tcttacacgc ttcaaataag acatttgcat acggacgcca cggtcttcct aaatagacgt
     4021 ttctagtatc aggttctgca gtcaaggtac agctttcaaa acgataccct gttttttgat
     4081 ttttcggcgt agatgctgct gtgatgtatc caggattttt actggctaag ctatggatat
     4141 gacactgatg aaaaacggca tctgccccac caaatatgaa atccaccgtt ccttcaatcc
     4201 agcaatattc ataggtctga cgacaatact caggattggc agaagccggt gtacaaaaag
     4261 gagtaccatc tttttgcaaa tcaggcaacg gacccgtgca taaggtatcc tgataaccag
     4321 atagtcgaca gttttttaaa gatacgcgat ctcctgaatt gaataaagca acagcctgtc
     4381 ctaccttttc tcctggacct gctgtatttt cgatgtgaag attttctaat acgacattcg
     4441 atccttctaa gaaaaaagta gcacttcgga aagtccctaa ctggctaccg tcaagatact
     4501 cctgttttgc aaaacgatta ccggagataa ctactgttcc tgctcccaca agtttaaaat
     4561 tggataatct gctttctata tattcatcat atcttccttc ttttactatc aagacctttg
     4621 gatggctatc cggcaggcct gccaaataat cgattccttc ttggatagtt tcaaaatccg
     4681 tcggggtatc tatagcattc tttccaatta tcagtttgtc tttcatcctc ataaatgctc
     4741 ccttatgaat tgtttccaaa tatttctcat ttcttgtgtt tccatatgac cgcctgttaa
     4801 agaaaaagaa gaaaagtttt ctggactacc cattgattga tactttttgt ccagcccttt
     4861 tctcagcttc agtacgcctt cgtccaagca catcgtatcg ttttttccaa ctaagctaag
     4921 acgagggcgc ggagcgattt tttcttggat tgctaacgtt gtataagctg tcaatagatt
     4981 tggcacatag taataaaacc catgatgatc taggcggcga tgtagcaaaa gtatttcgat
     5041 gtccacctgt gccgctagat caatacaaac tttgacacga gaatccaatg ctgtcaccca
     5101 ccagcttaat aagcctccca tagaaagacc aatcgttgcg agccgttgag tatctgtgtc
     5161 ttcccttgtc tccaaataat cgatcaagct gatcacatca taaatacgca ttccccataa
     5221 cgtttttcct gttaataaaa attgtttgaa caactcgctt tcagaaatac ctccgcgttc
     5281 ttcaaatccc caagcatcaa tcgaccaaac agcatatccc attcctgtca gtacttctgc
     5341 aaaagatgga gaagctaagt aaggagcacc atggatcaat tcacttttcc cgactgaaaa
     5401 atctccgccg tgagaatgca ggtaaaggac cggcggaaac ggtccttctt tttctggata
     5461 agcatagagg gctggtactg tttccaattc atttaagtac aattgatagt attcaagcat
     5521 aaaaccgttt tgtttttctc ggtataatcg ttttccacta ggggtatgtc tgctagatgg
     5581 agcacctaaa aaatcattca actgcataag caaagttttt acgaacaacc agttcatcca
     5641 tattttctga ttgatttgat tggaatttct caatactgat ttctttagta ttttctaata
     5701 gaaatgctgg tccctcgtga ttttcaatcg acatctggtt aaaggaaacc ttacccgcaa
     5761 agccaatata aaatccttgt ctgctcattt cctctattcc tgtcatcata gcaggaacac
     5821 cgggaactgc atcttgggcc atcgagacat cgatattgtc aaatgtgatt tcttgtatga
     5881 attgttccgc taatccataa ataaaacctg cagaagcatg gacattcctt gccgtgatat
     5941 tcgaaaaatg gatccttctg aatgctggtg tccgttcatc tatcggataa gccttttttt
     6001 cccatacata cggttctttc cctcgcggac cacagaaata ataaagattc agtataaatg
     6061 gacacatcac attatccatg actatattgg atactcgaat atcttcaacg atcccgcctc
     6121 gacctcgtct agatttcaat cgtattcctc gatcagtttc ttggaagata caatttgaaa
     6181 tggtaatatt acgaatacta ccactcattt cacttcctaa tacgacaccg ccatgtccat
     6241 ggaccatcgt gcaatttgtg atcgtgatat tttcgcaagc gatcctttcg tacgtatctt
     6301 cagtccctgc cttgattgca atacagtcat ctcccacgtc aatatggcaa ttactaatac
     6361 gcacattttt gcatgattct ggatcaattc cgtctgtatt tggtgaatcc gctgggttca
     6421 agatcgtcaa gttatcaaac gttgcattac tgcaaagaat cgggttgatc gtccaacttg
     6481 gtgactggat caatttaata tctttcacag taattcgatg acagttatgg aaactcatca
     6541 atttaggacg aggataagcg agattatctg gttcatttct aaaagtatgc caccattttt
     6601 ttccatttcc atctaatgta ccgaaaccag tgacagagat gttctctaca ttttgagcat
     6661 agatacagga agcataaact tttcgatgga ctccttccca acgtgaatga acaacaggat
     6721 aatcttttgg atcgtcagag aatttcagaa ccgctcctgc gcttaaatga agttcgacat
     6781 tgctttttaa aaataaagct cctgttagaa attcacctgc tggaacaact acggtcccgc
     6841 ctccgtcact tgctgcagca tcaatggctt gttgtattgc ttcagtattc aattcatcaa
     6901 ttgatgcgcc aaatgtcaaa atatcataca tatttataat gcctccactt caatcaatgc
     6961 ttgcaagaat gcaccaacgc ctttttgatc attacaaata attggttcac tgatgtagta
     7021 agcatatgaa ccatctcgct gatctgctcc gcctaaccct gctacttgac agtttttatt
     7081 caagttcagc cagccttctt ttgtttccag aacaaattca tcaagtaatc cttgatgtga
     7141 tttcttcact tgtggaagcc attcttcttt gttcagcagt cccaaacgta ttcctttagc
     7201 cattgcgtaa acaaacatac tactgccgga agcttccaga taatttcctt tccgttttgt
     7261 ttctgttgtt acttgatacc atgtcccgct gttttcttct tgatatggaa gtaatgcagc
     7321 cagtgtcttt tctagaattt ctttcagcaa agaacaatct gtttcttcat gaagtaattc
     7381 ataggtatct gctgcagcca tcaaatacca gccaatcgat cggctccaaa aatgatggga
     7441 tagacctgtt tttggatcag cccatggttg gaccttcttt tcatcccatg catgaatcag
     7501 caaatttgtt tttggatcga atagatgttt ataactgatt tcaaactgtc tcaaaacatc
     7561 ttggtaatct ttttcttcac taaaattctg gataaattca gcataaaaag gagaacccat
     7621 atataagcca tccaaccaaa tttgataagg ataaatttct ttatgccaaa aagctccttc
     7681 ggatgtgcgt ggatggtttg ccaactgctg ggaaaggagt tctgctgctt ttttatattt
     7741 ttcttcgcct gtttctttgt acaataaaaa gaaaagcttt cctgtattta catggtcgat
     7801 attgtattct tcaacgctgt agccacggat cgttccatct tcttgcacga aatagtcgat
     7861 attgtctttt atgaaatcaa aataaatttt ttcttgggtt cgttcccaaa gtaattggaa
     7921 ccctttaagt acgacgccat agtcataaga ccattttcct ttataccact tttcttcata
     7981 taagcgaggt gttctttcca tgatactttt tgctaatttc tttgaccagt tttccattat
     8041 ttgctctcct ttaaaaatta aaataggatt tgggacaaat accaagaagt atttgtccca
     8101 atcgtctata ttactgtgcc gctgctttgt atgcttcctc gtactcttgt gtgattcgat
     8161 cgcctccttg tgctttccag tcttctacag cttttttgaa tccatcttcg tcaagctgtc
     8221 ccatgatgta ttgcaccgta gcatcaatga cgattttttc aatttctgat cctacttcat
     8281 tgttcgtcgg tgaatccaat ggcacagaag gatccattac agcaaattct tcattttctt
     8341 tgatcaattg attagagaat tctttttcag ggtctgtatc tttgaaagag aacgtcactt
     8401 cactaggacg agagcttgat aacggttgca cctcttgttg ccaaagatcc atatcgataa
     8461 attcgataga accatcatca ttcaattcat aatgcgttcc ttcgataccg ccagtcatca
     8521 atttatacac atcttcttct aaaagatcat caacaaattg aagtaatttc ttcaattgtg
     8581 cttcgtcttt tacttgagat ttgggaaatg ctagcaagcc gccaatacca tttccttctg
     8641 accaaacatg ataaccgccg ccttcaccat ctgagatctt gtttacaggc actaattcca
     8701 tatcatcttg aataccttga cccatcgtct tcagattctt gatatccacc attccagtgt
     8761 agattccagt tcttccttgt gcaaattgtt cttgctgatc agtcttttgg gtaactgcaa
     8821 agtcttgtgc caaatagcca ttctcaaata gatctcttga aaaattcaaa gctttcatat
     8881 actcttctgt atcaaattct ggagtgaatt tgccattatc gtctacagcc cagccatttg
     8941 gtgctccaaa ataagaagtg aaaagtttaa agctggtata acgaatatca ctaggcgcac
     9001 gatcgccaaa accaactgtg tcgtccacac catttccatc aggatcgttt tctgtaaatt
     9061 gtttagctac ttcataaagc tcatccatgg tagttggaac atctagtcct agattatcca
     9121 accagtcttt tcgaatgact aagccagcac gagcgtagtc tttttgtatc ggtacgccgt
     9181 acaaggcacc gtcgatactc gcagcttcga ttctatcttc agaaatttga cttaaatttt
     9241 tatagtcttt taaatattct gatacatccc agaatacgcc tgattttaac gcattgcgga
     9301 cagaagagtt tttcaacatc gcaatttgaa gtgtaacgat atctcctagt tcatcagaag
     9361 ccaatgcagt tgttaatctt tcttcttttg aagcatcagg aatccagttg aatgtcagtt
     9421 ccgtatcggt tttttcttcg atcaagttca caatcgtgtc tgtaggcggt gaagcatgat
     9481 gcaaaatatt taaccaagtg attttagctt ttccatcttc tgtctctgaa gaactgttac
     9541 ttgaactgct gccgcatgcg gcaagtgcta gaattcctgc aaaactaaga cctaatttta
     9601 cgagcgttgc ttttttcata aattttctcc ttcactgttt taataatatt tttgttttct
     9661 tctgtttttt ctgtatatga gttcttccat tccaaatggc aaaatataga atttgctgtc
     9721 cacgtgactg cggaaaagaa gagactgcca ccgataaagg ttaaaacacc tggcattgtt
     9781 tggatgatca tcagataaat ccctaccata cttaatatca acactaatga gtaatgaaga
     9841 tgtgagaatc cgaataaaaa tgccattttc attttgaaaa aatatccttt ccaatcataa
     9901 tgagccatga ttgggaaaat atagagtgct gagagcacat atatgaacgc agcaatatac
     9961 aaagcgatcc taagatacca attgtgtatg aaagaaagat ccacaagtaa aacaatcccg
    10021 atggcgccat atatccagct catcagcaag gtttcacgat agttttcttt aaaatatgtg
    10081 aagtacgttt tcgtcaaagg aaacgtttct tttgagcgca gccattttct aatcactgca
    10141 accatcgcat acgatgcagg accgaaagta aaaactccca aacctgcgat gactaaaccg
    10201 ccccacagaa aattcaaata aaccaactgt aaaatacgat ttagccttcc gtttatttta
    10261 tctaatgact gcagcatatc tttccctctc tttctatctt agtaaacgcc gtcttctcct
    10321 agctttttgg caatcttatt agctaaaatt accataaaca atcctacgac ccctttaaac
    10381 aatcccattg ctgtactgaa acttaactgt ccattcttca atccagctgt ataaatatag
    10441 gtgtcgaaaa tttctgcaac gcttcgattc aatgaattca ataacaagta catatgctcg
    10501 aaccctaagt ctaatgtctg accgattttt agaatcagca aggtaatgat tactggacga
    10561 attgccggca atgtaacatg ccatgttttg cgtaagcgtc ctgctccatc catttctgca
    10621 gcttcataca actgtgtatc gactgcagtt atcgctgaaa ggtagatgat cgtagaccag
    10681 cccaactctt tccagatcac ttgtccgata tatactgtgc gtgtccattc aggggatgtt
    10741 agaaaactga ttttttcaaa tcccattgtt gcaaggaatt cattcaatac ccctccgtca
    10801 acattcaaaa atacataagt aatcgaaaca atgattaccc aagacatgaa gtgagggatg
    10861 taaatgatcg tttgaatccc gtttttcaat cgtttgttcg ttacttcatt taatagtaaa
    10921 gacaaaatga ttggcatcgg aaaaaacacg aagatattta atccgaacaa gatcaacgta
    10981 tttcttaata atgtaaagaa tgtcggttct gtaaacaaac gaatgaaatg tttgaaaccg
    11041 acccaaggac ttcccattat tcctaaaaat ggttgataat cttgaaaagc aataacgagt
    11101 ccccccatag gaagatacct aaaaatcaag aaataaagga ctcccggcag aatcatcaca
    11161 tataaaaatt tattcgtttt gattcctgca attatttttt ttcgtcttct gagcttttct
    11221 tcttttttcg atagcgtcag ttgaaatggt tcttgtttct ttacactttc cataagcccc
    11281 tccttagttt tgtgaatgtt tgctgcttat gacttcatga tagataacta tctgaattac
    11341 gtatataatc atttttaccg taagtaaaaa cgcttacata gcaatgattt ttgtgctata
    11401 caaaacgata aagtgtattt tttcacaaat aaactacgca tagcaaacct tctatttttt
    11461 tctatctgac acttagcata ggaaatgatt atatacgtaa tctttcaccg atgataaatt
    11521 gaacatatag aaacagaaac atttaattta ttttgaggag gagcctatct atatgaaaaa
    11581 gaaagtctct ataggagaaa gaagttttac acttttcaac aatacgtttc taattttgct
    11641 agctttgatc tgtatcgttc cttttttaaa tatcatcgct acatcttttg cttctacaca
    11701 agaagtcgtc gcgaaaaagt ttatcctttt tcctactacc ttttctttag acgcgtatcg
    11761 ctacattctt tctacgccga ccatttttag agcacttgct gtttcgattg gtgttacagg
    11821 tgtcggaacg atcgtcagta tgtgtgcaac ttcactgatg gcctacggtc tttccagaaa
    11881 gtatttgttt ggtcgaggtt tcgtcaactt tcttgttgta ttttctatgt tgttcagcgg
    11941 ggggatgatt ccaacgttct tagttgttcg ttctctaggg ctggtcaatt cttactgggc
    12001 catgatcctg cctgtggctg ttaatgcaat gaatatgatc attatgagaa actttttcca
    12061 agccttgccg gatagtttgg aagaatcagc aaaaatggat ggttgcacag attttggtgt
    12121 atttttcaaa attatgctgc cattagcgtt accgtctatt gctactatct cgctttttta
    12181 tgctgttact tactggaaca cttatatgac tgctattttg tatatcaacg attcatctaa
    12241 atggccgata cagattcttt tacgccaaat cgtcattgta tcaagtggga tgcaagcaga
    12301 aagtagtgcc gttgatgtga ttccaccagc acaaacagtt aaaatggctg ttatcgttat
    12361 tgcaacagtg cctatgctga ttgtttatcc ttttgttcag aaatattttg tcaaaggagc
    12421 actcgtaggt tctgtcaaag ggtgattatg tactgaacgc aaaatggtag ttgtgtcaga
    12481 agtggtcagc ttcaagaaat aagaaggcat ttacgaaaat tgcttcttaa atttttgtga
    12541 aatttcggct tatttttcga agaatctact cctggaacac cgtgtatcag aggttgtgaa
    12601 agagacgttc agccttgagc gatgataaaa actctcacaa acattgcgac gagtaaccca
    12661 caggagcaat aagaagcgat tttgaaaaat agcttcccat atttcacaaa attatgcctt
    12721 attgtcgata gcctgtctct tgaacgccat ttattcgtaa aaaagaactg tgacaagact
    12781 tttgtcacag ttctttttgc gtgcttagcc ttgaaattta actatccata aatacaaata
    12841 ctagggttat tctaacttct tttattgctt gaatcagtcc acaagaaaaa cagcctcttg
    12901 attcacaaat atttttttcg atattctccc ggcgtaatcg attccttctt tttaaagaaa
    12961 cgaataaaat tttgcggatt tcgatattgc aatctttctg caatctcttt tacggagagg
    13021 ttagtctctt tcaaccattt ttttgctacc tctaaacgat gattttgaac aaaatcacca
    13081 aatttttctc ctgtttcctt tttaaataca ttacttaaat agttagggtt ataatgcaac
    13141 ctatcagcga tcacatctaa agacagatca cgatcatatt ctgtgtagac gatatgcata
    13201 atattctccg acaaattttt aaattcatga tcagtgattg cttgagagca ttctgtgatt
    13261 ggcagaacta attgttctaa aacaagtttt tctagttctt tgggatgata attgttcagt
    13321 gcttttacat aaatttgttt catgttatca aagttttttg aatctactcc caataactgt
    13381 cctaattgaa cgagttcatt gatcaagcgg accgaaacta cttcccgact caaaggattt
    13441 ttattttgtg caaacaatgc atcaatcaat tcatgtacta aattcttcac ttcctgattt
    13501 ccagaacgaa tggcttcaaa caatcgattt tgttcttcta tcggatatct aataagtgtt
    13561 ttttcatgag cagcaggaac gatttcctga taaaaaataa ttgaacttgg accagtattt
    13621 acacgataat acagggcttc ttttgcccga tcaaccgatt gcttgctttc attcagcgtt
    13681 tcgaaccggt tactgatgcc tatactgata gttaaattca agtattcttt tacacgtttt
    13741 tgaatgagtc ggcaatactc catgactttt ttgcttgctt ctgcatcatc tttatccatt
    13801 cgataaatag tggcttgcat ttcttcattc aaaacgatcg gcagcattcg attttccttt
    13861 ggcacaattt ccgtgatcat gttgttgatc gctaaaagta gcaaatcacg ctccccaccg
    13921 tgactatctt ctaacagatc gatttgaacc aaagctgtat aatagcagtc attttgagaa
    13981 ctataaccaa attgctgcaa ccgctgatct agttctcgcc tttctactcg atttctaaat
    14041 aaactaagta caaatagcgt ttccagctgt ggcttttggg taactaagtg tgcacttaac
    14101 gcttcttttt ctcctatgat ccgactgact gattgagcga ccaaatctag ttctttccca
    14161 ctgaatcctt ttgatttaag attcagacgt tcctgtattt gagaaatcgg acgggcaaat
    14221 ctgtccgaaa ataagtagga aagaagacca atgagtccta ccagtgttaa agacactgca
    14281 actagcccga ttctcagatt gcggatcatt gcgccaattg cactagagtc aacttctaat
    14341 ctgtaaatcc aattattata atctgatttc agtactatat acgttttatc tttttctctt
    14401 atcactttca gctgattgta atctagatcc acgtccgtta atacagttgg ttgctcttta
    14461 ttttttactg tctgagtagt aaaaaggttg ccaacttcgt tttcaataga aagtatgtct
    14521 tctccacttt caccaacaac ttgttctatt gtatgtttct ctatcgtagc aattcccaat
    14581 gcatattttt ctttttcaaa cattggcaaa gaaatcacca tttcgattcc tttgtttttc
    14641 ttttgccaaa ataaattatt tttgttattt atgtacttct gttgatactt ttcaatttcc
    14701 tcttcggtca gttgactcaa cgatccattg attactcccc atttaccagc caaactgact
    14761 aaatcataat tattatcatc cataacaatc gtttctataa aatttagttc tttcctgatt
    14821 tcagaataag tggagtaatc tgtatatgtc agcggatgct tgaatatgtc cgagaaattt
    14881 ttggtcgaac catacgtaga aaacgaatat tcaatcgttc taattttcca ttcaacgtta
    14941 gttttaactt gcctgagcaa attctttttg tctgttaaat aactcgattc gatcgattca
    15001 taggtattct ggtaaatgaa taaactaaat cctatgacaa gtacggctcc taataaaaac
    15061 atcggtaaaa atattttata aaatactgta cttttcttca ttttcttcct ctcctccttt
    15121 taaaataaaa ggctttcatt tttctgtaaa attgatactc atctaattaa atgagcattc
    15181 cccgctttca agaatagcat aattaaaaat aaaaatccaa gtaattttta acgtgtgcaa
    15241 tcttttcata tcaaagatct attgacttat cgtagttatc gtaatatgat gaatgcgtta
    15301 acaaaaagtg aaaaagtaag gaaaatgatg gaatggtaac gatcaaagat gtcgctaaag
    15361 cagctggcgt ttctcatacg acagtttctc gtgctttgaa taacagcagt ttaataaaaa
    15421 atgaaacaaa acaaaaaata cgacaactag ctaaggaaat gaattattcg ccaaactata
    15481 gtgcaaaagg tttagtgaat caaaaaaaat atctaatcgg actatttttc tcaagtttgc
    15541 ataaaggaac ttcatcaaat ttcctagcag attccattgc aggcatccat tcagtattag
    15601 acacaaatta cagtttatct gttgaaagta tcgatgttgt tgatttaaca gaaatcaacc
    15661 tgcaacgcta cgatggactg atcgttatga gtcaaagtga tgatgatcag cctttcatcg
    15721 actatctcaa agaaaaagaa tttcctttcg ttgttgtcaa tcgtcacatc aatgatccag
    15781 cggttatcaa cgtcactgcc gatgatgcgg ccggtgttat gcaggcagta gattacggta
    15841 ttggatgcgg acatacgaag atcgcttata ttggcggaaa gaaaaatttt cgttcgacaa
    15901 aagaacggga aaaaggggta ttgaaaagtc taaaaaaagc gagcatccct gtcaatccag
    15961 cctatttttt aacaggagat tacagcttag aaagcggcct ccaaaacatg gaatacctat
    16021 tatcactttc tgacctgcct actctggttt tttgcggcaa cgacgacata gcgattggtg
    16081 cattgagagc tgtacattta gctggcctcc gtgttcccca gaacatttct ctgatcgggt
    16141 tcgatgattc tccagtcgtc tcctatctca atccgccgct taccaccgta cgtaagcctt
    16201 tgaaagaaat gtgtcaagaa ggaacaaagc ttttactaga tttgatcgaa ggcaggacga
    16261 tcgaacaatc taaaattgag ctctccacct tgttgatcct tcgtgagtcg atcaattact
    16321 tgtaaactac aagtaatttt atataaaaat gttaacgtgt gcataacaca gaaaggaagt
    16381 ttatatgaaa aaactgacaa aagaattagt gaaaaaaaac gaagcacctg taaaagttct
    16441 tcagtttggt gaaggcaatt ttatgcgtgg atttattgat tggcagattc agcagctgaa
    16501 taaccaacaa ttgtttaaag gtaatgttgc aatcattcaa cctttagaac aaggtcttgg
    16561 aaatatgatg aaccagcaag ataatttata tacggtcatt ttacaaggac tcagcaatgg
    16621 cgaaatcgtt gatacttccg agattatcac cgtagtagaa caaatgatca atccttatga
    16681 tgaatgggat gcttatctat ctctagcaga aaatgacgac ttggcctata tcgtctccaa
    16741 tacaacagag gcaggaatcc aattcgtgga gactgataat ttaacagata ctcccccaca
    16801 gagcctcccg ggcaaactga cagccttttt ataccgacgt ttcaaattaa acaaagctgg
    16861 ctttaccatc attccttgcg aactgattga cagaaacggc gaacgtctaa aagaaatcat
    16921 tttacgctac gcagaaaaat gggatttaga acctgctttt gttgagtggt tagaaaaaga
    16981 aaatgttttc tgttgtagtt tagttgatcg catcgtccca ggttatcctc gcgaagatgc
    17041 aggtgttttc aatcaacggc ataactatga agatcaattg atggtcaaag cagaaccatt
    17101 catgctttgg gtgatcgaag ggcctaaaga attggagaat caactaccat tcaaacaggc
    17161 tggcttgaat gtcatcatca cagatgatat gactccttat cgtgaacgta aggtgcattt
    17221 gcttaatgga ccgcatacag cgatggtccc tcttgctttg ttggcggaat tggaaactgt
    17281 agaagaagta atgaaagatc ctttattctc cgcatatgtt gatcagttat tcaatctaga
    17341 attgattcct atgctttctt tacctaaaga tgaattagcc atttatgctg accaaatcaa
    17401 agaacgtttc ttgaatcctt ttgcccatca caaattagaa gcgatttcat taaatagtgt
    17461 ttcaaagttc agcacccgtc tgcttccagt gttcaagaag tatatcgaag aacaaaatca
    17521 ggtaccaccg ttaatcactg tttcactagc tgcgctactt cttatgtatc gaggcgatca
    17581 agtaaagcca catgatgatg aaaagacgat aagcgaattt actgatgctt ggtctgataa
    17641 tggaacagct attcctcgat tattgcaaaa cgctgcgctt tggggagaag atttgtctca
    17701 aataccgaat gtgacagata cagttcaaga aatagctgca aagatcgatc aaaaaggtgt
    17761 ccggtcagta ttacaagaaa tgaaaggaac gatggtccat gcttgaaacc ctgcaaccaa
    17821 caatgaaatt aaatcgacga gacagtgtgg tcgtcgcatt aacccctatt ccaaaacaaa
    17881 cacaactgac gattgatcag caaacgatca ccactcttga agatatcccg caaggtcata
    17941 agattgcatt gggtgatttg aaagctggag acaacgtgat caaatatggc tatccaatcg
    18001 gtcatgtgac gacagacgta actgccggtc aatggctgca cactcataat gtaaaaacca
    18061 atctttctgg tgaattggat tatcgatatg aaaaagatgt ccatccaagt cattaccttt
    18121 ttgagaaccg aacatttcaa ggttacttgc gaaaaaatgg aaaagtaggg attcgtaatg
    18181 atttgttttt agtacctacc gttgggtgtg tgaatggcat tgctgaactg atcgtcaaac
    18241 agttcaagga aaaacatcca gatctgggac agtttgacca tattacgatt ttaaagcatc
    18301 cttatggctg ttcgcagctt ggaaaagatc atcaaaatac tcgagaaatt ttagcggacg
    18361 cagtaaatca tcccaatgcc ggaggagttt tagtatttgg attaggttgt gagaacaata
    18421 cagttcctga attcaaaaaa attttaggtg aatatgatga agaacgagtg aaatttctag
    18481 tggctcaaga tgtttacgat gagatcgaac aaggtgtggc tctattagaa gagcttttaa
    18541 cagcggcgga aaatgaccag cgcatatcag tcccgctctc taagttgaat gtcggtttga
    18601 aatgcggcgg ttctgatggt ctatcgggaa taactgctaa tcctttactt ggtgcatttt
    18661 ctgactactt gattgctcaa ggaggcagca cgatcttaac agaagtgcca gaaatgtttg
    18721 gcgcagaaca agtattgatg gcacgagcgg aaaatgaaga agtttttgac tcaatcgttg
    18781 acttgatcaa tgacttcaaa cagtatttct tgtcttacgg ggaaccagtt tacgaaaacc
    18841 catctcctgg taataaagca ggtgggatca ctacattaga agataagtct ttaggctgca
    18901 cgcaaaaatc aggaagctct cctgtcgttg atgtgctaca atatggcgaa aaaatcagaa
    18961 aaccagggct tagtttattg caagctcccg ggaatgatct tgtagctgcc tctgctcttg
    19021 cttcatccga ttgccagttg gtcttgttta ccacaggaag agggactccg tttggctcat
    19081 atgtcccaac ggtcaaggta tctacgaata caactctttt tgaacgcaaa ggtcattgga
    19141 tggattttaa tgcaggcgtc ttattagaac aaccaatgga agtaatctta gaatcgtttg
    19201 ttcaaaaaat catcgctgta gctagtggag aagaaacgaa aaatgaacaa aatgacgttc
    19261 gagaaattgc catttttaaa aacggagtaa cactttgatt ttcataaaac agtataaaaa
    19321 gaaagaagga agatttatgt ttttatctga tgatttttta ttaaaagacg attgggcgaa
    19381 aaagctttac cattcctacg caaaaaacat gcccattatt gattatcact gtcacttaga
    19441 tccaaaagaa atttacgaaa acaacaattt cagcaatctg acagaagctt ggttaggagg
    19501 cgatcattac aaatggcgct tgatgcgagc ttgcggcgtg cctgaagaaa aaatcacagg
    19561 aaatgcttct gattttgaaa aatttttagc ttggtgtcaa accgtaccaa aaattatcgg
    19621 aaatccgctt tacagttggt cacatcttga attgaagcgt ttcttcggta ttgacttgtt
    19681 gataaatgaa gaaaatgcag agaaaatttg gacacaagca aatgaaattt tggcagctcc
    19741 tgattttcgc cgtcgggaaa tcgcaaaaaa atcgaatgta gaagtgattt gtacaacaga
    19801 tgatcccgca gacgacttgc actatcatca gttgcttgct catgaagaac cagatttgaa
    19861 ggttctacct tcattccgtc cagataaagc actcaatatt gaaaaagaag gatttaatgc
    19921 atgggtcaca ctactggaag aagcagctga ccaaacgatc accacctatc aagaactgat
    19981 agaagtctta gggcagcgga tagaatactt ccatcaacat ggatgtcgtg tttccgacca
    20041 cgctttagac atcttacgct ataaagaagc ggatgaaaca gtattagaag agatttttca
    20101 aaaagcacga gctaacaaac tattatcttc agatgaaatc gatgcttatc gtacagaaac
    20161 actgatacat ttgacccact tctatcattc tcataattgg acgatgcagc tgcacatcca
    20221 tgcttatcgt aactgtaata cacaaatgtt ggaaaaatta gggcccgata ccggctatga
    20281 tggaatgaat gatctttctt tgactattcc tctgcaaaaa ttattgaacc gtgcagaaga
    20341 aacggatcag ttgccaaaga cgatccttta ttcattgaat ccgaatgact atcctgcgat
    20401 tttagctttg atgggctgtt tccaaaaaga acagaatgga aaactgcagt taggttctgg
    20461 atggtggtac aacgatactc gagctggtat gcgcgatcaa ttgacacaat ttgctgatgg
    20521 tagtgctttg gggaattttg tcggcatgct gacagattca agaagtttcc tgtcctacac
    20581 tagacatgaa tatttccgtc gtgttctttg tgaattttta ggcgaaatgg tcatgcgagg
    20641 agaagcacct gaagatgaac aacttttagg aagtctagtt caagcgatta gttatacaaa
    20701 tgctaaaaat tactttggct tttttgccta attgattaat agtaacagta acgaatcaaa
    20761 aacaggatag tgaaataacg gaatccgttt atccacgttt tagagaagaa gacggaccaa
    20821 atttgctccg tcttcttctc ttgctgttct gcttcattgc ctataaacct ttatgaaaga
    20881 agagataaca tgcctactat ccatattgcc ggtgattcta ctgcggcaga aaaagatgaa
    20941 aacaagcgtc ccgagacagg ttggggagaa aaaatctctg cttatttttc acccgagatc
    21001 cagatcaata accaagcaaa gaatggacgc agcaccaaat catttatcca ggaaggaagg
    21061 ttagcccgtc tagcttcaca gtttcagccg aatgatttct tatttgtaca atttggacat
    21121 aacgaccaga aaatccaaga agaaagaggt acgcttccct tcggtagcta tttaaaaaat
    21181 ctgcagatat tttttgaatg cgcaaaaaaa gcgaacgtcc aaatggtctt actgacatct
    21241 gtgacgcgac gagattactt agaaaacgga acattgaatc cagatatact tggagactat
    21301 ccaaaagcga tgcgtgcatt tgctgagaaa caccatattc ctcttttaga tgtgttctca
    21361 agatcacaag aattgttcca gaccttctca aaagaagaga caaaaaaatt ctacctgcat
    21421 cttatacctg atacttgtaa aaattaccca gaaggactaa aagacaatac ccactttagt
    21481 ccagaaggcg ctgataaagt tgcccgaata attgttgaat tgataaaaga atcccgtctg
    21541 cctcttgcta attatttaca aaaaggagtt tgagatgttt ccattaaatt taggcatccg
    21601 tgcccacgat ttagatgttc gaaatcgtga agaaatcgtt caaaaagtat cacagcttca
    21661 cttgtcccat atccaatttg caccacaaaa agcatttcct gaacaactta aattaactga
    21721 catgacactt gggagtgctg cctattttgg tgaatacttc caaaaaaaca atatcgaact
    21781 ttccatttta ggatgctata tcaacctatc cagcaaaaat caagaagttc gtgcacaagc
    21841 attggcaact ttcaaacagc aaattgctct gtgctctgcc tatcaagcca agatgattgc
    21901 tacggaaacc ggtagtgtct ctgtcggtta cacaaaagaa aattttactg aagaagctta
    21961 tcagatcgcc agaaattcta tcattgaatt agtcgctcat gcagaaaatt ttggcgtaac
    22021 tgttgctgtt gaagcaggga tcaatcatcc aatctataac tatcagttag ctaaacgttt
    22081 aatcgatgaa gtagcttcac caaatttaaa aatcatttta gattgtgcga atttgatgca
    22141 taaagaaaat catgctgatc aggaaaaagt gatccgaggt gccttggaca atttacatgg
    22201 ttatattaca gcgcttcatc tcaaagacta tatcatggaa aatggaaaga tccgcatggt
    22261 acctgttggc aagggctgtt tagatttccg cccggtactg cattatatca aatatgagcg
    22321 tcctctaatg tacgccactc ttgaagctac tcctgagatt tatgttcctg aagcaataga
    22381 acacttgcag tttatttacc aagattgtta aggagaagat tatggaggaa cgaatacaga
    22441 aaagttatca aaaatatcat gcaaatgctt ttcagacatt gctgcatctt tatcagggaa
    22501 attattggcg ttttgccatg tcaggactct tttttattat caaacatctt ccatcttggt
    22561 taatgccgat cgctattgcc aatgtaatca atgcagcttc atcgaaagat tcttctcagt
    22621 tgcaggccat ttatttcaac gcttttttga tggcttgtct gattggtcaa aatatattga
    22681 cgaattattt tcatgtaaaa taccatagca cttcgatccg tcaagtagaa gctggactaa
    22741 gagcgaccct tatcaagaaa atccaagaac tatctattcc ttatcaaaaa gaattgcaat
    22801 ctggacgaat ccagtcaaag atcatccgtg atgtagaagc tatccaaacg ttgtcctctc
    22861 aagtatttgt cagcggatta aatatcgctg tcaatttatt cgtagcttta ggtatcacgc
    22921 tttataaaag tcctgttgtt ttcatgtttt tcttattgac tgttcctctc gccgcattcg
    22981 ttactctctt tttccgtaaa aggatcagac agtccaactc agacttcagg caagaaatgg
    23041 aagagacttc tgcaaaagtg atcgaagtga tcgaattagc accgattgct cgagctcatg
    23101 ggttagaaaa acacgaaaca actcgcttaa ataagcattt tactcaaatc tatgaaaaag
    23161 gattccactt agatatcctg caatctgttt tcggatcagt cagctgggct agttttcaga
    23221 tatttcagct gatttgtttg attttcactg ggatattagc tttacaaggg cgcatccaac
    23281 ccggcgatgt cgtgatgtat cagacgtatt tcacgacgat cgtcaattcc gtttctggaa
    23341 tgatcacgct gattcctacg atttcaaaag gcatggaatc tatcacttct attggcgaac
    23401 tgttgattgc tggcgatgta gaaacttatc acggtaagaa aaaaatccaa catttggatg
    23461 gaaattttcg ttttgaggat gtttcttttc tttatccgga ctcactcaaa ccgattatcg
    23521 atcatttttc tctagaggtg aaagctggag aaacaattgc ttttgttggg ccatctggct
    23581 ctggaaaatc tacactcctt aacttattga ttggatttat ccagccgact tcaggaaaaa
    23641 tttttctaga tgatcagcct ttggatgaac tggatatgcg aagtgtgcga aattatttag
    23701 ccgttgttcc tcaaaccact ttgttgttct ccgcttcgat caaagagaac atcacttatg
    23761 gcttgaaaaa cgtatcaaaa gaacgtttag atgaagtcat cgaagctgcg caactttctt
    23821 cactgattga ccagcttcct gatggtctgg ataccttagt aggcgaacat ggcaacaagc
    23881 tgtctggtgg ccaaaagcaa cgtatctcca ttgcacgagc gttgatacgt cagccaaaaa
    23941 tcattttgct agatgaagca acctcagctt tagataacca gtcagagaaa aaaatccagc
    24001 aagccctgaa ttacctgaca gaaacaccca ctacttttat tgtggctcat cggctttcaa
    24061 cgatcaaaga ggcagataaa atcgttgtga tcgatgaagg aaaaatcgtt gaaataggta
    24121 cttacgaaaa actgttagct caaaaaggct atttctaccg cttgaaaaat gaatgacaaa
    24181 aaataggagc tatgacaaga cttttgtcgt agctcctatt tctgaataaa cggtgttctc
    24241 ctgccaactc ctcggtcaca agtcacaata gtcatcaaac atgagaagct gttttttgaa
    24301 attgttcctt gtaactcaga gttaaacggt aggctcacga cctcttggta aatggtgttc
    24361 aagagacagc ctatcggcaa taagaagcaa ttttgtcatc gctcaaggcc gaacgtctct
    24421 ttcacaccct ctgttgtttc ataaaatagt tttattcgct gtaaaggtcg cgaactggtg
    24481 tcatgatgat acggttcaag tcattgatga ttgtactgaa gccttgctct ttttgcatca
    24541 agtcattgat tacttcttct gattggattt tcacagccat ttcttgtgct ttcattgcat
    24601 cttcgtcgct gaactcttgt ccactcattt gtttttcttg taattcgatt tgcatcgctt
    24661 ggaagtcttt gaacagtttg tgtgcttctt cgtttgcttg tactttctcg tacgcttctt
    24721 tcaacgcttt gaattctggt aattcacgga tctcacgttc gatttgattc gcgctatcgt
    24781 agatgtttga catgttatgc cctcttttct tttagtagtt actctctatt gtaacatggg
    24841 gttattcgtt ttcaaagggg ctactctcct tgatatttcc gccagaggtc tttagccgct
    24901 tcaccaattc cctgaacgcc ttctttcacc atatcgccga agtttttcag cccttctttt
    24961 gcttgttcac catattgatt caagttatct tgccaatttc cagactctcc attagaattt
    25021 cctgtgtttc caacttgatc tgcagctacc acttgtcctc ctgtggcata ggcatctgct
    25081 acactaaatc ctgtttttga agtatacgga aggatgcctt ctgccgctga tttgaagact
    25141 ttcccgacaa catttccact tgtcccttcc aaatagtgca gcttgctgga ttcttcgtaa
    25201 cccatccatg tcgaaatcac tacgtctggc gtgtaaccga tcatccattg gtcatttgct
    25261 tttgtggcat cgaaattagt ttctgtggtt cctgttttcc ctgctatcgt ataacctgca
    25321 ggattcgcag cagccgctgt tccatttgaa aatgtaccaa gaagcatgct ggtcatttca
    25381 tcggctgttt cctttgatat gacctgtttt ggttttttgt ttgtgttatc gacgatcacc
    25441 gctccagtcg aatcaacgat tttcgtgata agatgcggcg tatacatctg accatcattg
    25501 gcaaatgcac tgtaagcacc cgccatgatc aatggagata ctcctttttc cagtccacca
    25561 agggctaagc cataatactt atcagatttt gctaaaggca ggccgaattc ttctgcttta
    25621 tcatatcctt tttgtaaacc gatctcatgc aaaagccata cagtaggcaa attcaaactt
    25681 tgtgcaacag cttgatacat cggtacttct ccttggtatg tcccgtcaaa attatgcgca
    25741 tcgtaataac tttgcggttc gtctttcaag atactggaag gcttgtatcc agcttccagt
    25801 gctggtgtat atacgcttaa tggtttgatc gttgatcctg gtgaacgacg catctgtgta
    25861 gcaaagctga atcctctaaa gacatgatct cctcttcgtc ctactagtgc ttgtacacca
    25921 cctgtattcg gatctaaagc tactgaagca gattggacca tggcaccatc tgaagcattt
    25981 gctgggaata atgcactgtt ttgataagta gcttctaatt gcttttgata atcctgatct
    26041 aaagctgtat agattttata gcctttattc atgacatctt cttcatcaag accatactcg
    26101 ttaactgctt catcgatcac tgcatcaaaa taataaggat aacgataacc agaatctgaa
    26161 ttatcgtatg tgtcattaag atacgcgctg atatctgtct ggctttctgc ttgttcttgt
    26221 tcttttgtga tttttccgtt ttcttccatt acacttagta ctgtgttttt ccgatcattc
    26281 gcattttttg ggtgatcgat cggattataa ataccaggac ctttcagcat tcctgccaaa
    26341 gtagcagcct cagacagcgt aacattcgtt gcatctactc caaaatactt tctggaagcg
    26401 tcttggacac cccatacccc gttgccaaaa taggcattgt taagatacat ggtcaagatt
    26461 tcttctttgc tgtacttctt ttcgatctcg attgccaaaa ataattcctt cgcttttcga
    26521 tcaaacgttt ggtctaatgt cagatacgca tttttggcaa gctgctgagt gatcgtactt
    26581 cctccgcccc ctgatgttcc gccagacaag accattcgga ctgctgcacg ggcaatccct
    26641 ttgatatcaa acccgtgatg ctcgtaaaaa ttcctatctt ctgtcgagat tactgcatct
    26701 tggatatagg gagaaatcgc gtttaattct acatatgttc ctttttgggc gtacagtgtg
    26761 ccagcttctt cgtttttctt atcataaatg actgtcgatt ggcttaatcc tgattttaac
    26821 gtttcgacat ttgcttgttt tgctaaaata aagaggtaga tacttgtcac taagacacag
    26881 atcaaaccaa tcagcaaaat caatttgttc acatgatatt ttttccagat gcgctttcgc
    26941 catcgatgga attgtccgag atatggtctg atccaacgcc aaaaactgat caaagcagct
    27001 tttatctttt ctaaaatgct ttgaaaatcc atttgcttct tctcctgtct atttgattca
    27061 atcgtcttat tttaccatag tgaaagagtg ttttcttcct gcttaagttg tagttcaata
    27121 tgttttttta cgttagtgca agtagagtaa cataaga
//
LOCUS       DAXWLF010000019        26560 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXWLF010000019 DAXWLF010000000
VERSION     DAXWLF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.019"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            162..593
                     /gene="mraZ"
                     /locus_tag="ACGE4U_000612"
     CDS             162..593
                     /gene="mraZ"
                     /locus_tag="ACGE4U_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFF8392799.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            614..1573
                     /gene="rsmH"
                     /locus_tag="ACGE4U_000613"
     CDS             614..1573
                     /gene="rsmH"
                     /locus_tag="ACGE4U_000613"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFF8392800.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1578..1979
                     /gene="ftsL"
                     /locus_tag="ACGE4U_000614"
     CDS             1578..1979
                     /gene="ftsL"
                     /locus_tag="ACGE4U_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFF8392801.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            1980..4172
                     /locus_tag="ACGE4U_000615"
     CDS             1980..4172
                     /locus_tag="ACGE4U_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFF8392802.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4198..5160
                     /gene="mraY"
                     /locus_tag="ACGE4U_000616"
     CDS             4198..5160
                     /gene="mraY"
                     /locus_tag="ACGE4U_000616"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFF8392803.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5174..6535
                     /gene="murD"
                     /locus_tag="ACGE4U_000617"
     CDS             5174..6535
                     /gene="murD"
                     /locus_tag="ACGE4U_000617"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFF8392804.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6561..7649
                     /gene="murG"
                     /locus_tag="ACGE4U_000618"
     CDS             6561..7649
                     /gene="murG"
                     /locus_tag="ACGE4U_000618"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFF8392805.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            7664..8884
                     /locus_tag="ACGE4U_000619"
     CDS             7664..8884
                     /locus_tag="ACGE4U_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFF8392806.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9040..10365
                     /gene="ftsA"
                     /locus_tag="ACGE4U_000620"
     CDS             9040..10365
                     /gene="ftsA"
                     /locus_tag="ACGE4U_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFF8392807.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10388..11629
                     /gene="ftsZ"
                     /locus_tag="ACGE4U_000621"
     CDS             10388..11629
                     /gene="ftsZ"
                     /locus_tag="ACGE4U_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFF8392808.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            11646..12323
                     /locus_tag="ACGE4U_000622"
     CDS             11646..12323
                     /locus_tag="ACGE4U_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFF8392809.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12337..12936
                     /locus_tag="ACGE4U_000623"
     CDS             12337..12936
                     /locus_tag="ACGE4U_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFF8392810.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13017..13238
                     /locus_tag="ACGE4U_000624"
     CDS             13017..13238
                     /locus_tag="ACGE4U_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFF8392811.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13287..14069
                     /locus_tag="ACGE4U_000625"
     CDS             13287..14069
                     /locus_tag="ACGE4U_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFF8392812.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14090..14797
                     /locus_tag="ACGE4U_000626"
     CDS             14090..14797
                     /locus_tag="ACGE4U_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFF8392813.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15059..17857
                     /gene="ileS"
                     /locus_tag="ACGE4U_000627"
     CDS             15059..17857
                     /gene="ileS"
                     /locus_tag="ACGE4U_000627"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFF8392814.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(17906..19432)
                     /gene="zwf"
                     /locus_tag="ACGE4U_000628"
     CDS             complement(17906..19432)
                     /gene="zwf"
                     /locus_tag="ACGE4U_000628"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFF8392815.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19447..20094)
                     /locus_tag="ACGE4U_000629"
     CDS             complement(19447..20094)
                     /locus_tag="ACGE4U_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFF8392816.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20278..20607)
                     /locus_tag="ACGE4U_000630"
     CDS             complement(20278..20607)
                     /locus_tag="ACGE4U_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFF8392817.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            20784..21512
                     /gene="sfsA"
                     /locus_tag="ACGE4U_000631"
     CDS             20784..21512
                     /gene="sfsA"
                     /locus_tag="ACGE4U_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFF8392818.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21528..22541
                     /locus_tag="ACGE4U_000632"
     CDS             21528..22541
                     /locus_tag="ACGE4U_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8392819.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22541..23818
                     /locus_tag="ACGE4U_000633"
     CDS             22541..23818
                     /locus_tag="ACGE4U_000633"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8392820.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            23881..>26560
                     /locus_tag="ACGE4U_000634"
     CDS             23881..>26560
                     /locus_tag="ACGE4U_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8392821.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMF"
BASE COUNT         8987 a         4584 c         5681 g         7308 t
ORIGIN      
        1 gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt
       61 atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt gggggattgt
      121 ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg ggtgaattcc
      181 aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg
      241 gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat ccgatgagcg
      301 aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta
      361 cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa caaggacgaa
      421 tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc gtagtcatcg
      481 gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt tctgcagcag
      541 cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta taagaatagg
      601 aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga aagaaacggt
      661 agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg gcggtgccgg
      721 acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg cgtttgacca
      781 agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg aacaaggtat
      841 ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg agcatgtaga
      901 taaggtagac ggcattttgt atgatctagg ggtttcctct ccccagctag atgaagcaga
      961 acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc agtcagctcc
     1021 tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa agattttctt
     1081 ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa
     1141 aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag tcatcccagc
     1201 tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc
     1261 agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc ggttattgaa
     1321 agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa ttgtcaaaag
     1381 tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg tcatacctga
     1441 cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt cagaggaaga
     1501 gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa aaataagaat
     1561 gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa tatgatttac
     1621 ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat caagaaatca
     1681 ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa aaaattgctg
     1741 tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg ctgcgcacaa
     1801 atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat gataaaaagc
     1861 aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa cggattaaac
     1921 aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa
     1981 tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat ccgatgaaca
     2041 atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattgttc tttttatttg
     2101 ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca ttagctgaaa
     2161 aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga acgatttatg
     2221 atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa gcgatactct
     2281 caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat tttgataaaa
     2341 tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg aatattttag
     2401 aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg aaaaatatct
     2461 cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt
     2521 attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct cattttattg
     2581 gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt ttggagtctg
     2641 cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa gataattttc
     2701 aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga caagatatct
     2761 acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat caagtaaatg
     2821 aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct
     2881 tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg actggaaaag
     2941 atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca accatgaaag
     3001 ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa accttccaat
     3061 ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt gaaaaaggtg
     3121 tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc atactggaac
     3181 agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga caaagtactc
     3241 attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt gtcgaccgag
     3301 caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg aaagctttca
     3361 cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa gtggtggatc
     3421 cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct ttttcaaaag
     3481 aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa aactacggta
     3541 ttgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaacc ggaacagccc
     3601 aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg tattcgattg
     3661 tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg aaacacccta
     3721 agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg
     3781 attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa gtatccgtag
     3841 ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa agtggactgc
     3901 aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc
     3961 agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg atgcctgacg
     4021 taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt attgaagtta
     4081 gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg
     4141 aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga aaagagcatg
     4201 gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat tgccgcaatg
     4261 ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga
     4321 ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt gtttctgatc
     4381 ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac tcctactctt
     4441 ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga tgattttata
     4501 aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat tggacagatt
     4561 ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg tgtattgaat
     4621 ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct cttctggctt
     4681 gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt tgctggctta
     4741 ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca gtatgatgta
     4801 ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta caacagaaaa
     4861 cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact tttagcggca
     4921 atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat ctatgttatg
     4981 gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa acgaattttt
     5041 aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg gaagattgat
     5101 acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat cgtttggtag
     5161 gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg
     5221 actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg cacttgtcac
     5281 cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc tttctttagg
     5341 gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat tttcattgat
     5401 ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc aagaaatggg
     5461 tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc caattattgg
     5521 gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac tactgaatgc
     5581 aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag caagtggtgt
     5641 agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct tccaactcat
     5701 gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg aagcacatat
     5761 tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc aaaaaaacat
     5821 gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag aattagcgca
     5881 aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag acggtgtgta
     5941 tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat ctgaacttgg
     6001 cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag caaaattgta
     6061 tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg taccacatcg
     6121 tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa aagcaaccaa
     6181 tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg tgttattggc
     6241 tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa aaggaatcaa
     6301 agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg
     6361 catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag cattagaaaa
     6421 cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg atcaataccc
     6481 aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa aatagaaaaa
     6541 gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca gggggacata
     6601 tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat gctgaattca
     6661 tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt
     6721 tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac atcaagacgg
     6781 tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat tttcaaccag
     6841 atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct gcagccaaaa
     6901 tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc aataaatttt
     6961 taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc ttttttcctg
     7021 aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat actaaaaaat
     7081 cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg atttttggcg
     7141 gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt gaatatattc
     7201 cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag atcctaaata
     7261 aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat atggcagagg
     7321 tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc gcagaattca
     7381 cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat gatcatcaga
     7441 ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca gataatgaac
     7501 taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa gaattgcaaa
     7561 agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa cgtctttatg
     7621 acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga
     7681 accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc
     7741 taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa aagaagacat
     7801 aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag aggaacagca
     7861 agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa
     7921 cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa gtcaacggca
     7981 caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac cgctgatttt
     8041 tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat caggcaataa
     8101 gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag aaaatgtttg
     8161 gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac agccaagaat
     8221 cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg taacagaata
     8281 taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg aaaatggaac
     8341 agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg aagagttcaa
     8401 agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat cagaattaca
     8461 aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc tgatccaatt
     8521 gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga accaaatgaa
     8581 atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg
     8641 tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc aatcggaaga
     8701 aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag aggcagaaac
     8761 gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt caaatgattc
     8821 aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt taaatgaaaa
     8881 ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa tttatggtag
     8941 aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg ctagttgttc
     9001 gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac aggaatgtat
     9061 gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata tatcgacagc
     9121 caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg aggcattatc
     9181 gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc agaagaaaaa
     9241 gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa
     9301 aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga tgaagatgtc
     9361 agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca
     9421 atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg
     9481 atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac gatcatccat
     9541 aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt gattactcct
     9601 ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac gatcgtcatt
     9661 gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt gaagtttacc
     9721 aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt attaaatacg
     9781 tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc agaacggaca
     9841 tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt gaaagtcgat
     9901 gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa caaagcaaaa
     9961 gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt gacaggcgga
    10021 gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa
    10081 ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt aataagtatc
    10141 gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc tgttacgggt
    10201 gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac atatgagagc
    10261 tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc cggagaaaat
    10321 gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat cctgggagga
    10381 ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat
    10441 cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg
    10501 tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa aagctgaaac
    10561 agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc agccagaagt
    10621 tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag acggtgcaga
    10681 tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg caccaatcgt
    10741 tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac gtccattcac
    10801 ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa
    10861 tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag ataaaaagac
    10921 accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg tacaaggtat
    10981 ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg tgaaaacagt
    11041 gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag aagatcgcgt
    11101 agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta ttgatggagc
    11161 tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg aagcacaaga
    11221 tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc taggtacatc
    11281 tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag gtatcgatga
    11341 atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc aatcacctgc
    11401 tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag aagaaaacag
    11461 ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg atgattccaa
    11521 ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa caaaatctca
    11581 tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag
    11641 agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc
    11701 ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct gttgaatccg
    11761 ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac cgagtagata
    11821 aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg catttaattg
    11881 gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac tttcatgcgt
    11941 tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt
    12001 ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca gatgagcttt
    12061 taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg ttgatgacta
    12121 tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg ctgaaagaat
    12181 tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg
    12241 ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc cgtgtaggaa
    12301 cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa cagaggagca
    12361 attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga ttatcctgaa
    12421 acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca atcaactcat
    12481 tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg
    12541 catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca atcacctaca
    12601 aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa agcaaatctt
    12661 gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc gattgctaaa
    12721 cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga aaaacaagcg
    12781 agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga tatcaaacgt
    12841 gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag ttcaaccgct
    12901 caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg atttatacta
    12961 ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt atcttgatgg
    13021 tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga cgattattag
    13081 ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg attggaccct
    13141 tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa ggtttagcac
    13201 agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa aatctttatt
    13261 ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac tttcgggcag
    13321 atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa ggacaatatg
    13381 caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa acactgattc
    13441 gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca gaaagaaaaa
    13501 gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa acagcattgt
    13561 atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc cttggaactt
    13621 tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac ggtgatcgct
    13681 ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta acaaagattg
    13741 gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg ccgaaagata
    13801 gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg atctctgaga
    13861 tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa gtaaaagtga
    13921 actggacaga aaatacaaga cctgattttg tacttgaact attagatatt atttcagtca
    13981 gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca
    14041 gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca tggctttaac
    14101 tccattagat atccaaaata agacattccc gacaaaaatg cggggatata atcaagacga
    14161 agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc aaagaaatcg
    14221 agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca atgaattaaa
    14281 agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag taaaaacaag
    14341 tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag atgagttagt
    14401 tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa aagcaagaaa
    14461 aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg aagatcttaa
    14521 gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc aattagaaca
    14581 agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg tgactgacag
    14641 ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg atactgatgt
    14701 aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac aggctattga
    14761 tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac tttgttattc
    14821 ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa agatcctcta
    14881 cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg ttattggtca
    14941 cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc atgttcgtcc
    15001 cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat
    15061 gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc
    15121 aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca
    15181 agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat atgccaatgg
    15241 taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca ttcgttcaaa
    15301 atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg gattgccgat
    15361 tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt
    15421 ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag attttaaacg
    15481 cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt cttatgaagc
    15541 tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaagc tatatttaca aaggattgaa
    15601 accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa ttgaatataa
    15661 agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca aagatattct
    15721 tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc cagcaaacca
    15781 aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg gccgtaagtt
    15841 tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg aaaatgtaac
    15901 agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc atccattcta
    15961 tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg gtactggatt
    16021 agtccatact gctccaggac atggggaaga tgactatatc gctggtagca aatataaact
    16081 atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac ctggatttga
    16141 aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag aaaaaggagc
    16201 tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga cgaaaaagcc
    16261 tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc gtcagaatat
    16321 tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac gtttgtataa
    16381 tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg gagtaccact
    16441 tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa tcgaacatgt
    16501 ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag cgaaagaact
    16561 tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa aagaaaaaga
    16621 tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac gtcagcgacc
    16681 agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc gtggctggtt
    16741 caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca aatcagttat
    16801 ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt taggaaacat
    16861 cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct tatgggtatc
    16921 tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc aagtggcaga
    16981 agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag aagatttcga
    17041 tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat atatgaccgt
    17101 tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat ataacttcat
    17161 gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat tctacctgga
    17221 ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc gttgcatgca
    17281 aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta tcattccgca
    17341 tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc agttaagcga
    17401 gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct ggactgcttt
    17461 catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca gcaaattgat
    17521 tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc gtgaaatgtt
    17581 aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact actttgaagt
    17641 tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg ttgctattac
    17701 agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta aagatgtagg
    17761 agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg aagacttcta
    17821 tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg
    17881 ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca cgataccagt
    17941 tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa gctgctttag
    18001 gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc caagcttttc
    18061 gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag aagttcgttc
    18121 catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta ttttcaacca
    18181 tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct tgccctactt
    18241 ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc gttagaacat
    18301 taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat acattaacag
    18361 gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt ttgcctgtac
    18421 gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg cctgcgacga
    18481 atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc ttgaattgta
    18541 caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac acttcttctt
    18601 cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt tctttttcgg
    18661 aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata tgattttgca
    18721 ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct acaccgaggt
    18781 tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga gcctcaaaaa
    18841 tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct aaataatgat
    18901 caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc aattcaaaag
    18961 cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag ccattttttg
    19021 tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag
    19081 ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt
    19141 cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag taaaaatggc
    19201 ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt tctttgacaa
    19261 cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca gcaaaatgct
    19321 cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta cgctgggcta
    19381 ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac atgtggatac
    19441 actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga cggggatttt
    19501 ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa cgatctcata
    19561 ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt tttcatcaag
    19621 cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt gtcggcgttc
    19681 ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat gttcaggatg
    19741 attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt ccaatacttc
    19801 agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa aaacttccca
    19861 taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag tcaattgtac
    19921 accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa tcatttcact
    19981 gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga ctttcgttgt
    20041 atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg tcatattcga
    20101 tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac gttaattgac
    20161 tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa aacgaaagat
    20221 gatagagaag actcattctt tcgtttataa aggatatata tatacattgg gagggatcta
    20281 tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag tttgttcggg
    20341 ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat attcatctat
    20401 ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat cagatgtaat
    20461 ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat actttgagag
    20521 tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg agagaacggt
    20581 aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc tttatgagaa
    20641 aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa aagctaaaat
    20701 ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga taggatacat
    20761 agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct ttttttattg
    20821 accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata attattgcgc
    20881 atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa gtggctattg
    20941 tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta aaaaagaagg
    21001 gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat ggtattttag
    21061 acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag cgtgaagtaa
    21121 cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa aaagcatttg
    21181 ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct gatgcgccca
    21241 cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat ggttatgccg
    21301 cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc catgaagaaa
    21361 tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt caaatcctcg
    21421 cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt ccatttgatg
    21481 tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg atcaacttag
    21541 gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta agcacaaaaa
    21601 aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa tttggttctc
    21661 attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat attactcata
    21721 tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct aagcaagcaa
    21781 ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca aaagaaatgg
    21841 atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca gcaagaaata
    21901 tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac gagataatcg
    21961 gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta gatggagaag
    22021 aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta ggtgtttacc
    22081 ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat ttcccaagaa
    22141 agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag aattttgatg
    22201 tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt tattttgact
    22261 caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt catgatcgcg
    22321 cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg gcagatgaag
    22381 cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga ttgttgtatg
    22441 aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta agaaaaaatg
    22501 gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat ttgtacaagt
    22561 atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac cttctcttat
    22621 tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg tttctcctac
    22681 aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg aaccaggtga
    22741 agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag tagcagatgt
    22801 tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg gcggagccaa
    22861 cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg gtacaccagg
    22921 aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga aaacaatggt
    22981 atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg atcaaattct
    23041 tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag atcgttccct
    23101 tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga cagcagagga
    23161 cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa agaaagatga
    23221 ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct ttaatcaact
    23281 gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg taggaagtct
    23341 ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca aaaagaaaat
    23401 ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta caggtcttcc
    23461 ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc gttcgggaag
    23521 aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata ataatctaag
    23581 agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt ttttatacgg
    23641 aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag taaataaaaa
    23701 gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag aaaaaataag
    23761 aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc
    23821 gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag gaggggttat
    23881 gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca aggattgaca
    23941 gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg atatttaagt
    24001 gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt ttactctttg
    24061 ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca agtgactttt
    24121 gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc aaaagctgcg
    24181 attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg tacagggaag
    24241 tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct ttctgcagat
    24301 gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact gctctttgga
    24361 cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag tcccggtcta
    24421 atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt accgccggaa
    24481 gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct tggagaaagt
    24541 gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc ttcagaaagt
    24601 ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa tcttgcttca
    24661 cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt
    24721 ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg
    24781 gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa atctttttta
    24841 cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact ttcccttcaa
    24901 gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg attcatggac
    24961 agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg gtcacaagat
    25021 ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc tctttccgaa
    25081 accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga tgcttatttc
    25141 cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt gccagatgaa
    25201 atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga ccgtatttat
    25261 aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga tcgtgtgaga
    25321 gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt taagagggaa
    25381 ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt tgagattcct
    25441 tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga aagtgagcct
    25501 gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac tgcttcaagc
    25561 atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt taatatgcca
    25621 ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa
    25681 gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa caccttcagt
    25741 gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac aagtaccatg
    25801 attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga tatttatcaa
    25861 aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa
    25921 actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc
    25981 aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact gatccagatg
    26041 cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga agaatatgag
    26101 atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt tttttcagcc
    26161 tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga tgatgagatc
    26221 ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt ttctattgat
    26281 gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt gtcgattatc
    26341 gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt agacggattg
    26401 atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa aatttatcgt
    26461 ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata tcaggtaaaa
    26521 attccagaag atgatatcgc atttcttaca caaatgtttc
//
LOCUS       DAXWLF010000020        26456 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXWLF010000020 DAXWLF010000000
VERSION     DAXWLF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.020"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(191..1135)
                     /locus_tag="ACGE4U_000635"
     CDS             complement(191..1135)
                     /locus_tag="ACGE4U_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFF8392822.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            1356..2570
                     /locus_tag="ACGE4U_000636"
     CDS             1356..2570
                     /locus_tag="ACGE4U_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8392823.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            2592..4607
                     /locus_tag="ACGE4U_000637"
     CDS             2592..4607
                     /locus_tag="ACGE4U_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HFF8392824.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(4690..6063)
                     /locus_tag="ACGE4U_000638"
     CDS             complement(4690..6063)
                     /locus_tag="ACGE4U_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFF8392825.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(6089..6373)
                     /locus_tag="ACGE4U_000639"
     CDS             complement(6089..6373)
                     /locus_tag="ACGE4U_000639"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8392826.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(6375..8390)
                     /locus_tag="ACGE4U_000640"
     CDS             complement(6375..8390)
                     /locus_tag="ACGE4U_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8392827.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELWSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(8569..8874)
                     /locus_tag="ACGE4U_000641"
     CDS             complement(8569..8874)
                     /locus_tag="ACGE4U_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFF8392828.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(8963..9994)
                     /locus_tag="ACGE4U_000642"
     CDS             complement(8963..9994)
                     /locus_tag="ACGE4U_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8392829.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10201..11076
                     /locus_tag="ACGE4U_000643"
     CDS             10201..11076
                     /locus_tag="ACGE4U_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8392830.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(11228..12070)
                     /locus_tag="ACGE4U_000644"
     CDS             complement(11228..12070)
                     /locus_tag="ACGE4U_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8392831.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(12162..14039)
                     /locus_tag="ACGE4U_000645"
     CDS             complement(12162..14039)
                     /locus_tag="ACGE4U_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8392832.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(14036..15457)
                     /locus_tag="ACGE4U_000646"
     CDS             complement(14036..15457)
                     /locus_tag="ACGE4U_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8392833.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(15460..18849)
                     /locus_tag="ACGE4U_000647"
     CDS             complement(15460..18849)
                     /locus_tag="ACGE4U_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8392834.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            19106..20497
                     /locus_tag="ACGE4U_000648"
     CDS             19106..20497
                     /locus_tag="ACGE4U_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8392835.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(20570..21217)
                     /locus_tag="ACGE4U_000649"
     CDS             complement(20570..21217)
                     /locus_tag="ACGE4U_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8392836.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(21225..21911)
                     /locus_tag="ACGE4U_000650"
     CDS             complement(21225..21911)
                     /locus_tag="ACGE4U_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8392837.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(21917..22990)
                     /locus_tag="ACGE4U_000651"
     CDS             complement(21917..22990)
                     /locus_tag="ACGE4U_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8392838.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            23212..23886
                     /locus_tag="ACGE4U_000652"
     CDS             23212..23886
                     /locus_tag="ACGE4U_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8392839.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            23891..25231
                     /locus_tag="ACGE4U_000653"
     CDS             23891..25231
                     /locus_tag="ACGE4U_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFF8392840.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(25380..26366)
                     /locus_tag="ACGE4U_000654"
     CDS             complement(25380..26366)
                     /locus_tag="ACGE4U_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFF8392841.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         7711 a         4988 c         4838 g         8919 t
ORIGIN      
        1 ttgatggaaa cgaattcctg aatacgcata acagataaat gacaaatgtt tacacctata
       61 tcccctgata atacaaccaa aagtttagaa ctgaaaataa atccaaaaaa attcgctgca
      121 acgatataat aagacagcaa tcgcacctaa aacaattttc cttcatatgc tttagtgaaa
      181 gattttcttc ttaatgttta tttctccatt cattgggagt acatgttgaa tacttcttga
      241 ataatttgaa taaagaagtc gtttgtttat atccgatacg attagcgatt tcttccaacg
      301 aaaaactcgt ttcttgaatc agttccaacg caaagtcgaa ttttttcttt agaaccagtt
      361 cttgaaagga agcatcaaaa tacattttca atttattact aagatagacc ggattgtagc
      421 caaaatgagc tgctgtttct tttaacgtac aagaagtaaa atgcttatcg atataatcta
      481 atactctaag catctcattt tctgcgcatc catctgctga tgaaaagctt tgttgttcta
      541 ataaatgagt caattcgatc agtaatagtg atgtatagag atttaacgaa cggatcttat
      601 gactgtatgt atcaaaataa atcaacagca tattttcaat caaatgcgtc aaacgacttg
      661 tttttccgcc gcgaaaatga atgaattgtg tatgttcccc tttttctttt gatgcattta
      721 tcaaaaaaga agaaagtaga ttatttgtgc ttgccaaatg attcaatatc tctgtagata
      781 aggtttcttc ttttaataag atgttgatta aaatatcctc ttgccctgtg tattcgattc
      841 tttgagtagc ttctttatca atgatcacca cgtcactttc ttccatctct atcctttctc
      901 cattaataat ttgtaaacat gtaccgctat acatataatt gatttcaaca aaatcatgtg
      961 tatgctcggg aacttccgca tatcgacggt gtttactgat atgaatattt ccctgttgaa
     1021 atagatcccc tcggtggatt tttggcggtg tctgaagatg gtaagctaag tttaaatcat
     1081 tgatattcat tccttgttca cgctgcagag tttcgattga ttcttcgtta cgcaaaaaaa
     1141 atgtctaatt cagctagttc caattgactc cctccttttt gtaagcggtt tctttcgctt
     1201 attagttcac gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc
     1261 tatggttggg acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta
     1321 tatttttgat aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga
     1381 aattttctct gctgtcagtc tctcttattt tgacttctgc tggtgcagtc acgtctgcaa
     1441 ttcccagtat gtcagctact tatgccacgc gttctttatc tgctgttgaa ttattaacga
     1501 ctgctccctc ttttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa
     1561 tcggacaaaa acaaactgtg atcatcggat tagtgattgc atcgatttat ggtatcctcc
     1621 ccctcttctc acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct
     1681 ttggattgat caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac
     1741 gagcctctat gattggtttt caaagtgcat ttcaaggatt tggtgcagct ttaatgacat
     1801 ttgttgcagg acagctagta cagttcggtt ggcaatatac ctttttagtc tacgccatca
     1861 gcttacctat tcttgtcttg tttgttttgt ttgttcctac acctgaaaca actgaacttc
     1921 aatcacatgg cacagcccca acaagacaag taatcaaaac gaatcctaaa atgattagtt
     1981 atggtatttt tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt aaattagcca
     2041 cagtcctgac gacttattct ctaggaaacg aaactaatgc agcgaatatc ttaacaatca
     2101 tgcaattggc aagtatggct gccggagttt gttttggctg gcttcagaca aaagcacatc
     2161 gctatatgct tacactttct ttactactga tgggaatcgg ctttgtattg attgccactg
     2221 tgataaacct gtatgtgatt gggtttggtg cgatcctaac aggaatttct ttttcgttat
     2281 tcattcctta cttattcaac caagtaagta tccaagcgcc caagtacgcg gagagtttca
     2341 atacttcaat cttattggtt ggcgctaatt taggtacttt cattgcacca tatggtttgc
     2401 tctttctctc ttttgcttct cttgggagta aaacacttgc tgttttcgtc aatggcagta
     2461 tcttgctgtt gattagcgcg cttattagcc ttctagtagt acacaggacg aatagaaaga
     2521 cagtgataag agtagatgaa gaaataaaag aacaattgac aacaaaataa atttgatgga
     2581 ggtcgtgtaa aatgacaaat gaaaaacata aagatagatg gctttggtat cctggagact
     2641 ttgagatccg ccatggactg ctacaaaatt tccaacggga agaacgaggt tttgactggc
     2701 cagcgtactg gtacatggat gattgccatc ggaacgtcaa gtttaagaga tattactttc
     2761 ttgatcaacc ttccatgttt aaagtgacga tccaaggagt tgggtatgta gaaattaatg
     2821 gacaaaaaca tccatgtgga aaatggctga cttgtccagc tggtaaagca aaaattcgta
     2881 tctttgtagg acatacatca gggctaccag cgatgtttat cgagggagac gaagtaaaat
     2941 cagatattgg ctggacagca agtaatttta ttgaagaata tccagctggc tggagtccgc
     3001 tttatgtcga tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc
     3061 cagtcactga aacggagatt ggcggaggaa tcttattcga ttttgggcga gcagtgaatg
     3121 gctgtgtaga actaatatct ctggatgacc atatcgaatc tatcactcta tgttatggag
     3181 agtccgatgt agaagctatg gatatcgatt attgctacta taagcaaata ggaatgacaa
     3241 gaaaaatgaa agctcgtaaa cgcgcttttc gctatttgtt tataccagag gtttctactg
     3301 gaatggttca acttcaagtt tttcatgaat ttttatctct ttcgaaacaa gctgcttttt
     3361 caagcgattc ttctttactg aatcagatct ggtcagtatc agaagaaacc ttcaagttat
     3421 gtagtgattt gttttttatc gacggtatca aacgagatcg ctggatttgg tctggagatg
     3481 cgtatcaatc attcatgatc aatcaatatc ttttttttga cgaggaaatc aataaacgaa
     3541 cgatattagc attaagagga caaaatgaaa tcaagcagca tatgaatact atcgttgact
     3601 attcgatctt gtggttgatt ggtatcgaaa accactatat gatgacgaaa gactatgaat
     3661 ttttacgcat cgtctaccct aagatgcaat cattaatgag atacttgatg gagcaaacga
     3721 atgaattagg gtttatttat gggagagcaa aagattggat atttattgat tggtctgaaa
     3781 tggataaaga aggaacgctt gctgcagaac aagtcttatt actgaaagcc tatcattcca
     3841 tgatcacttg cggagatatc ttagctgaaa catatggaga gaacgaaaat tatcatactc
     3901 atctctatca agaaaaatat caactattga aacaaaacct atttgactac ttttgggatg
     3961 aagaacttgg cgcttttatc gattgctatg aatctgggag aagaaatgtg acgcgtcatg
     4021 cgaatatttt cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa
     4081 agaatgtctt attgaatgac actatcacac agattactac tccatatttc aaattctatg
     4141 aacaagatgc tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg attcttgatt
     4201 attggggagg gatgttatct catgatgcgg taactttctg ggaagaatac gaccctactc
     4261 aaacaggaaa tgaaaagtac agtatgtatg gagatcctta tggaaaaagc ttatgccatg
     4321 catggggagc aagcccaatc tatcttttag gacgctattt tatcggtttg tatccaacaa
     4381 agccaggtta tgaagaattt atgatacaac ctaatctaga ttcatttgaa acactgaatt
     4441 gtacgctacc tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga
     4501 ttacttctga tagaacaggc ggaacagtga aaatgaatgg taaaagttat cctcttctag
     4561 caaaacagcc acttgtctta acaagctatt caaaaataaa tgcataaaac aaaaaactgc
     4621 ttgcgacata tcagaagact ttaaatcttc ttctgatatt gtccaagcaa tttttatttt
     4681 gaagtttgct taattctgtt ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa
     4741 gctagataat acgacaagta aaaagaaagc gatgattcca actgtcaagg ctatttgacc
     4801 gtattctcct agtttcccaa gtacaagtcc tggtactaga taatcagtat cggcaaatgt
     4861 agaagaagcg attttaagat ctcctaatac tggaaggata aataatggca gccaactaat
     4921 caataagcta ttgaaagcag aacctaatac cgcccctctt ctaccgccag tcgagttgcc
     4981 gaagacacca gaagcagctc cgcagaagaa atgtcctact accccaggaa tgataactgt
     5041 agtttgcaag cctatcatag caaacatgga tagtgttcct acaaagaagc taacaaaaaa
     5101 gccaattaat acagcattag gtgcatatgg aaaaacgata ggaacatcta atgctggttt
     5161 agaatcgggt acgagttttt tagcaatccc ttggaatgct ggaacgattt ctgccaacac
     5221 cattcgaacc ccttgaagga cgataacaaa cccagctgtg aatgttccag cttggatcag
     5281 tgcaaagatt acagcgttgt ctccattgct tagttctctt tcaacaaatc caggaccagc
     5341 taacaaagca agtgcgacat atacgatcga catcaagagc atgatcgata cagtagaatc
     5401 tcgcaaaaag ctgaaacttt taggaatctt tatgtcctct gtagaacggt ctttgtttcc
     5461 aaaaaacttt ccgatccatc cactagaagc ataaccgata tttccggtat gccctaacgc
     5521 aacttgattg tttccagtga tgttcttcat aaacggttga caaagagcag gcgtaatagt
     5581 taatagtgtt ccttcgaaga ccccgcctaa taagatcatc cacaaatttg cagtaaaccc
     5641 cgcgcttact aaaatgactg ctgttagaca agaaacataa agcattgctt gacctgtaag
     5701 aaaaatgaat ttatatcttg tgaatctagc aagtaaaata ttcacgatca tccctactag
     5761 catgatcaaa gcagtaggtg taccgtattg ttgcaaagca atcgccacta ctgcttcatt
     5821 gctaggaact actccttgag tatgcagagc tgtttggaac atcgaagcaa aaggggttag
     5881 tgaattactg attaaaccag caccgccgat cagtactagg aatccgacaa atgttttgat
     5941 agttccttgg atcacttctg aaaatttctt cttctgaaga acgagaccaa gcagtgcaat
     6001 caaagaaacg aggatggagg gctgactgac gattgacaca aacaaattca acaatttatc
     6061 cattgatagt tcctcccttg aatcattttt attcttttga caagatcttc gttacttttt
     6121 cttttacttc ttcgctgtca atgatcgatt gcaaaggtac gagcttttca tcaggtatag
     6181 aagttagcgt atctttcaaa gaatgctcaa tgaagaaata atcagccata tcgctagata
     6241 cacttccaac atccgtatga cttacttcaa actcgtccac atctgctcca agatctgcaa
     6301 gtgcttgttt gatgttcatc tctaccataa aacttgttcc taatccactt tgacatactg
     6361 caaaaatttt catatcattc gtctcctttt cttatgatcg ctaatatttc ttcttttgtt
     6421 gatgcgtgta ataaatcttg aagattttgt ttattagaaa gtagctttgt taaatttgcc
     6481 aaagcccgaa gatgtgcctc attatcgata gcagctaaac agatgaacaa actaatggga
     6541 tgggctggat tatcaagcag atccacaggt tgttgaagtt tcagcaaaga catccctact
     6601 tcgtttacgc catcttctgg acgagcatga ggtaatgcga tgtgatcgcc aatatggata
     6661 aacgcaccat actgctccac gcgctcgatc atagctttga tataactttc ttctatttta
     6721 ttttctgata atagcggctg tgcggcaaat gtgattgctt cttgccagtt ttttggttga
     6781 tccgataatt ggatcatttc ttctgttaat aattcagata gcataggacg ttgatcctcc
     6841 ttcttttcta aaagtttgtt cattttacga tttaatactt gatagagctt ttcttcgtct
     6901 acgccttctt ttaattcgat ataaggcagt agcgccgacc acaattcttg ggcagaaggc
     6961 attgaaaatc ctggtatcag ccaatctctt tgtacttgat tgatcagctg atttttttcc
     7021 aagtcagtca tgattggatg cagaacatag agagactttt tcttatcagt aagtgtgaga
     7081 ggtacagtag aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct
     7141 gaattagtct ctgtaaaaaa gatcatcggg aataattgct gaagttcact ttttaagatc
     7201 aatgaagagc taatgccact tggacagacg atcacggctc ttatttttct atttctttct
     7261 tctgcgtccg cttttctgat ctctccgcca aatagtatag tgaaaaaacc aatctcttct
     7321 tcagggatgc tcttccctat tcgtctttct aacggaagta atgcttttct tgtcatttcg
     7381 tagatggaag catattgatg cttaatttga tcgatcatca cattaggtaa ataaaaagaa
     7441 taggaaatac gaaaataggc agggaccaag tgataaaaga cattcatcaa taattcacga
     7501 aacgaagcaa attctacggc catcaggaat tccatgcgat ggatgacttc tgaagaacat
     7561 tccagcaaaa aatcaagtgc aggctcctgg acatctcctt caacgatcga tgctaagcaa
     7621 agagagaaaa atataatctc cgaattttga tgttcggaat agccaaggcc agtcaatact
     7681 tgtcgactaa tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt taccagttcc
     7741 atggatgttt ttccaattcg aatggttgtc aaactcaaat aataagctaa ttcatccatt
     7801 cgatttggtg cgaatcgaag atctgatagt gaaatcgttt tatctagcaa atctctaact
     7861 ttggcgtaat aatcaatgga aagggttgcg gttaaatacg tgagcccaaa tcttccagca
     7921 agtgactgca tcaaacgagc agtgcgattt tttgcttctc ttcttaatat tgcttctgac
     7981 cctagcagcc taaaaccagt tttcctagta tacgacagct gacaatcttc cacttctaat
     8041 tcatttctta tctttttgat atctgacagg actgtccctt tagaaacctg aagaaaatct
     8101 tgaaaatgaa aaacagaaac agacgttgca gtcgaataaa ccatcaaata aagaagatct
     8161 cttcgctctt gttcggtata cacagcttca aatcgagtaa tatccaaaaa tttttctgcc
     8221 cagtcgattg ctcggaattt ctttatacgg atacgggtat catccacata ttcggcaaaa
     8281 ggagttaatt gtttgatcgc ctcgcttgta tttacttcga tgatctctgc tgctttttct
     8341 agactgattt ctcttggtgt tttcaggaga taactaattt tgtagttcac agcgctgttc
     8401 acctcctact tatattctag tatggtagcg cttttaaatc gaggggtgtt tggtacaact
     8461 tacagttgga ttttttttac gactgtaccg aacgatttct atcgaatgga tacagaaaaa
     8521 aagcaggtga ccaaatgtct cctgctttta tgaattgatt tcgtccgatc aatgatgatc
     8581 atgtttattt tttgaggtca gttcttcaaa tttttctttg acatcttcaa ccgctttttc
     8641 gatatctttt tcaaagtgat tgggctcatt aggctttgtt tcctcttcta cagaatttga
     8701 accagataaa gctgtttctt tattttctgt ttcaccagag tttgtgccag ctaatgattc
     8761 ttctttgtct tctgtttcac cagaattagt tcctgctaaa gccacttctt cttctgtttt
     8821 aggggccatt gaacctctta atcgtttgtg tttttcaccg tcataatttg tcatgttggt
     8881 tcctcctagt agttctatac tttttcatcg tacaataaga cagtttatgc gtcaaaaaac
     8941 aaacccgatt cttacaaata tgtcatattt ttccttctga ttcttgaatg cttttgattc
     9001 cttcttccaa aattttcatt tgtaagattg tttcttcatc tttgatgact ttttctgcgc
     9061 cattaactaa tgtctcgtaa atcccttcgt agacacggct gtaatcacca acctcggaaa
     9121 ttactttttc ttcatgataa ctcccttgtt catctatata ggtcaataca ccataatgtt
     9181 cgggcaaatc gataccaaag tcttcatgat caggcatgta aaacattttc agatgttctt
     9241 cttgtcgatc ttttgtttgt ttcacgaaca tccctttttt tccgtaaacg acgaaacttg
     9301 gacgttcttt gatgcggaaa taactggatt ttatcgaaat tttcatattt ccatagaaaa
     9361 aatccagatc gaagtaatca ttcattcgtc ctttccctaa aagttgacgt acatcatagc
     9421 tgatatcatc tggttcgccg aaataagaaa gaacttgatc tactgtatga caagcatgtc
     9481 catataaata gctgttcgca gtagaaaatt catgcgtatt ttctggtact tccggtctga
     9541 aataatcata atgcatttct acttctaaaa tatcccctaa ttttccgctt tcgattactt
     9601 tttggactgt caagaagtca gaatcgaacc gtctgttttg gtaacattgg ataaatagac
     9661 ctttttcttt tgctaatgca aataactctt ctgcttcctt tgacgtttct gcaaaaggtt
     9721 tctctactag tacattctta ccatgttcga gtgccatttt tccatattca taatgaaatt
     9781 gactaggtgt agtgatgaca actaattgga tctcaggatc atcccaaaga tcagacagct
     9841 gatcagtata ttcgatgtct tcgaatgttt tccacactgg ttcagatggg gaaaaaattt
     9901 ttttgatatt gattttgttc gtaagttttc ttgaaaaagg caaatgatag cgatttgtac
     9961 tctttccatt gccaagataa gcgattgtaa gcatgagtgg tgcctccttt tattgatgtt
    10021 ttcagtatat cctagaaaag cgcagaaagt atttataata gctattctgg taaaaaagtt
    10081 cagaaaaaca cttgttatca ttcggtttgg tgaaattaat gttcaaagta aggataaaaa
    10141 tcaaaaagat agtagcggaa ccaagtgttt atcagtaaga tagaatttgg aggtcagatc
    10201 atgcttattg aagaaaaact aacaaaacaa gagttgttta ccacgacaga gaaacggatt
    10261 gtcgattaca tccgtaggaa tatcgaagct gcagtctaca tgacgattga agaattagca
    10321 aaagctacgt atacttccca ttcagctatc attcgtttat gcaaaaaaat gggattcagc
    10381 ggatttaaag aattccgttt cgagctgggg cgggaagttc atcaattgat tgctcagttc
    10441 aatcaaacga ttgatcctaa ttttcctttt tcaagtgaag atagcactca gcaaattgca
    10501 aagaaaatgg cggatttatc gatccagtca ataaaaaaag cacaattgca gattgaacat
    10561 gaagatttag atcaaatcgg taaagtattg aacaaggcac agcgaatctt tctttttgca
    10621 aaaggtgatt ctcaaatcac ggcaaggaaa ttccagaata aaatggtcaa gttgaataag
    10681 tttttgatca tggctgaaga atacagtgat tcttcttgga atgcagccaa tttgactagc
    10741 gaggattgcg ccatttttat tagctacagc ggtcgcatcc atcattatga acgaataatg
    10801 acctatttga agcatgtaga ggcccctacc ttgctgatta ccggaaatca gcattcagaa
    10861 atggccaaac aggctgatat gtgtcttgtt atatcgcagg aggaatatga ttttgctaaa
    10921 gtagcgactt tctcctctca aattgccttc gattacgtat taaatacgtt attctcagtg
    10981 atatattctc aaaattttga agagaatatc cgcaacttga aaaacaaaca agaattgatg
    11041 caaaaaggcc ttcttaaaga atatcggaaa acttaagaaa agatcctcat ttagtaataa
    11101 gaaagatagt gtttgatagg ctctctttga tatcatcata aaaacaggct gaagttttct
    11161 ttttcaaacc tcagcctgtt tgtttacgga acgattcgaa tagatatatt atagaaatga
    11221 ggaatagcta acttctgttt cttattttga acgattgacg aaaaagatat ataagaagga
    11281 ggaaaatcag tatacatgca gctaacaatc catacaggaa aaatcgtttg ttctcggata
    11341 ctttctgtac ttctttttga acttcttttg catgataagg aacccggtgc cctctgacaa
    11401 gtaagcgatg actgttgacc atataaggtg tacaagttag gagtgtcacg aaatcctgcc
    11461 ctttttcgat tctcagatct tttgtatcag tcggttctac cgtcttgatc tgatcgactt
    11521 gataagcgag tgtccgtcca ttgatttcga tatagaaaga atcacctaaa gtcagtttcg
    11581 gtaagtctgt gaacaacttt gcttgaggca gtcctcgatg gctggaaata acggcatgtg
    11641 tattttctcc ccccgtagga taagacgttc cttccaataa tgaggcacct ttttctagca
    11701 gcacagtatt cgtttcatca aaaattggca aatggacatt gatctcaggg atcgtcaaaa
    11761 taccgatcgt atgtttctca aaataggttc tatccttttt cgcaggttct tttttcttag
    11821 taaagggatc tgcacctgga tttccacctt cttttgctaa ttgctggttt ttctttgtca
    11881 tgttttcttg gattttagcc atgacttctt cattttcttt gaccgcttgt tgctgataat
    11941 gagagatgat ctgctgatcc agataattat tcagcgcgtc gctaacaaat ggataagcta
    12001 gtatccctat tcctgagaga agcaagaaaa gcatcagcag atctagaagt ttccttttct
    12061 ttggtttcat ttgtgttctc cttgtgatga ctgtttgata ataaaagaaa aaggagcatt
    12121 tgcgtaatcc gctgctcctt tttccaattc ttgcaaaaag actaaatctg actgtgcttg
    12181 cgtctagcaa agtacagtcc agcaatcagt agaaggacta ctcctgcacc gatatacaca
    12241 tagattccct taccgcctgt tgaaggaagt gtacctttgt gtttatttgg tattttttca
    12301 ggagaaatca actgtcctgc tgttacataa gattgttcat cgatagtaaa tgctacacgg
    12361 tttgttaatg gaacatattt ttctggcgct ttcgtttctt ccagatagta cgtgccatat
    12421 tttagacctg tcacatcgat taaaccgtta cttgtggttg taaaaaccgt tgctgattct
    12481 ttcgccgata cccagctgac ggcttttgtg gatgggtcga tcgataaata tttcgctgtg
    12541 tcactatctt gatcacgaac gacgaattct gctccagcaa gtgtttggtc tgatgtaacg
    12601 tcaccatcta ctttaacaaa tcgtttgccc ccagtaacga catcgactga cggtggtgtt
    12661 tgatcatttg tatgcccgtt atcgacattt gcttggttag aaaatccttt ggttggatct
    12721 gctttttcgt tcaaatgcat atagtaaacg aatttcaatg taccgccagg agttaatgaa
    12781 ggaatataat tcggatcaac tgaaaccgtg aatccgtctg tttgctcagt gacagaataa
    12841 tttactgggt cgatttcttt atttccatca tataaggcat aagcatacgt tcctgaagaa
    12901 tcattatcaa atgttaaagc agcgtcatga gtatcgataa gtttgaatgt tgtgtacttg
    12961 ttttgcgttc cttctttatc agcgatacct aatgggatat tgacagaaat ctcatattga
    13021 attttttcac cgattgcgac atcttttcca ttcaattgag gtgttgtttt atcaacttta
    13081 gacgtatcat ttttgatggt cttttctact ggtgtttggc tatttgccag gatctcaaaa
    13141 ggcgtgattg tttgcttttc aatcatttcc gcattatcag gagcttttac ttcttctaaa
    13201 ttatattttc caacttctaa atgattaacg atcaactggc ctttttcaat agatgcctcg
    13261 gcaaagtcat tgttgccgat gtcataagaa tgaccagtaa tgaaatgttt ggctttggtt
    13321 tgatcagttg tccaagtgta caatccatct gtgacacttt ggatgtattt tttgacgctt
    13381 ggtgttcctt cttctttaga aataataaat tctgctccat ttagtgcttc gttttcggca
    13441 gtaccgattt ttgtaacttt caacgttcca tcattaccga ctgtattttt agggtagaga
    13501 tggatagtat ctagttcttc tgtcccgtat ttataagagc catctgcttg tttgatcatc
    13561 tcatatacag ggaaagctaa aaccatgttt gcggcagctg acactccgtc ttttggttct
    13621 tctttgatcg tgtagactgc atctttccca ttttgttttt taggtaaaga taaagtgaca
    13681 tttccatcag catctgtcgt tcctgaagca actggtgtac caggagtcaa agactggact
    13741 gcttgttttg ctgcatccac ggacgctcct ttatctcgtt gcgcataaaa ttcttgagtg
    13801 acgttataaa ctgaaaatga aatatcggct aatccttggt attgatcgaa ttcgctcatt
    13861 tctttcccgc tgttttggat taaaggatcg ggtaaatcag tcattttctt tttatgaagg
    13921 atgacttgag ctgctgtctc ctctgcaaac actttttttg cgccgaagct gtttgtgagt
    13981 aatggtaaaa taaggaaaag gactcctaac ataacgttta tttttttatg atttttcatt
    14041 ttttcactcc tgttcttttt ttgataaaca accagattgc aaagcttgcg aataccatac
    14101 caacaacgcc taaaagtgta tttttcgttt cgtttgtttt tggaagaaat ggacggctat
    14161 tttctttttt attgactact ttcaatatac tgtccgcctc tttatcctca gaaagaaaag
    14221 ttgaaatggt aaacggaatt tctgtttcac ttaaaatata accagaaggt gcttcgatct
    14281 cttttaatcg ataacttcct gttttcaacc cggaaattga aaaagcgcca tttttatcgg
    14341 aaatcaggtg aagcgcatct gattcgttct ttgtccactt gtatccgttg gctgtttgct
    14401 ggaggtaatt tcctgctttg tttttaataa ggaatactgc gccagctaat cgtttatctg
    14461 gtttatttgt ctcgattttg acaaattgct tactccctgt atatacagag actttctttg
    14521 tactgacacc aaaaccaaaa tccagtgttc cgttgttgtt gatttccttg ttcgcctgtg
    14581 ctgtactgct tagctgcatc tgataagaaa tggtcagttt ttttccagca tatttttcca
    14641 acgaagggat cgaaaataaa acatcaaatc cgtgaggttt cttgtgtatg acaaagcctt
    14701 ctgtcagctt ttctccattc gatgaaatcg ttaaactatt aggtaaaaac gttaatgctt
    14761 catccgcact atctgacaat tcgaactttt gatagtcaag gatatttaca ggaattgtcg
    14821 tatgcaagga ataagtgatt ttttcaccaa tcgtaaaatc atttctaggc tcttctaata
    14881 ccttctcaaa aggtggatca gggtagtcgt tttcttcatt tttaggatac aaatggatag
    14941 ttgaaagttt ttgattgttt tgtccatgta caggaagaac aactaccata ttctctgcct
    15001 tttctttgac gacttctggt actttggatt caatgaaaag atacgcttta tctctttttt
    15061 cttgatcttt agcggccaga gaaaaagaag cgataccatc ttcgttgtta agagttgtag
    15121 ttgttgcttc tgcggtaaac ataccggaag acgcaccgtt ttttgcgatc tctgcttgtg
    15181 cttcttctac cgttttgccc ttttcccgta gatggtaaaa agaatctgtg acatcataaa
    15241 cttggaatgt gacaccattt aatcctcgat acgtttgtaa taaagctttt tcttgtccgt
    15301 catttggatg atttttcggc agttgcccgt ttgggaaaag caatttgtgt aaagtaatct
    15361 gaaccatttc tgtttctgtt tctgttgcta cctgagtaaa agctggttta aatagtaaca
    15421 agaagagaca catactaagc caaccaagtt ttttcatatt tagctgcccc ctctttcttt
    15481 tttcaaaaaa agataccaaa tagaaaacgc acaaccaatc atccctacta gatagattcc
    15541 taaacggcca attcctcccg tttcaggtaa tggtactttt gcctgattcg tgatgtctaa
    15601 agaaatctgg ttgtttttgg ctcctggtga cagaacagat tcatgatctt gtccatccac
    15661 ttgaattgac ccatcttcgt gtataactat agtaactggc tcttttagac cttggtatcc
    15721 ttctggtgta aaagtttcgg tcagcgtata agttccaggt tttaaatttt cgaatagaaa
    15781 ggtatctgtt tcttgtccat cttttggcga ttctaagtcc atttctggtc cctgcagtct
    15841 gaattttgct ccttttaatg tctgtccgtt gtcttctttt ttgtgtaccg ttagatcaaa
    15901 cgcttttagt cgattttgat gagtgagcgt ccatacatta tttgtcatct cggtattttt
    15961 tcccgtgtaa atgatttttc catatttatc gatttggaat tcataatttc ccggcgaagt
    16021 atcatatccg gcaggaccag ctgtttcaac gactcgatat ttaccaggtg tgagtgaatc
    16081 aaaactggca agaccttttt cattcgttgt ttggctgtca attggctgat aagtaccatt
    16141 tgcttctttt ttctgtaggg aaaatgctgc tcctttaaga tttatctctt tgtccgtccc
    16201 ttgcatcgca tacttacgta ctgctacagg aacttcaaca aaaggatttt caatagtcaa
    16261 ctggatcgta tcatcggaag tagtgactgt ttttccatca atggttaccg taccatcaga
    16321 agcgatcttg atttcccaag tagtcttttt gcttaatcca tgcccttctg gagcttttgt
    16381 ttctgtcagc gtataggtca tttctttttg caatttgaca ttttctggaa gagaataggt
    16441 tccgtcgcca tgatctgtta gtaaagtatc aatagaatct cctgttaatt tgaaaacagc
    16501 gccaataaga tccttttcac ctgtagagga atttttcgtt attttcaagt ttaacggtgt
    16561 gaattgttta gtatttttcc atgtcattgc atctatttgt tgagaatcgt atcctggtac
    16621 aggtaattct ttgattgttt ggtaactgaa tgcttgacct tgattattgt attgaggtag
    16681 tgataatatc tcttgataac tttctccgct atttgcagat aatttgtcaa tgtctgcacg
    16741 ttcccacgta tttgttgtat ctttagctgg tttaatgatt cgaatatatc cgtttttcca
    16801 agctgcagca tttgtcgtat gttcccgttg aatctcaaaa gtaacttgat ctggacgatc
    16861 agctagattg ttgtcaaatt cttcccataa ctttttgatg tgaagactga ctccgggagc
    16921 tttagcagat ggtataccga attcagctaa ttcattggta tcaatgtttg gctggaaagt
    16981 tgtcctgccg ttcatttgat accagaaatt tggatggaag tcctcatttt ctgtttggat
    17041 tctcacttgg taatggattt ggatttcttg gtcttttcct aaatagatct gattgctctt
    17101 gatggtattt ccttctatgg aaatagatgg agtgaccgtt gtaggacttg tccccacact
    17161 cttgactgaa agtgtaccag gctgataaat gaatggttct gcgattggat catttatttg
    17221 tccattgctt acagttgctg agatctgtac agcttttttg gctaggtatt cagagatatc
    17281 tgttgcatga tcagctgatt catagtaaag atcgcctttt tcatctgatg aaaccatttg
    17341 tcgcatacga gactctactt ctgcttttga gagaccagct gccggatcgc tttgaagttg
    17401 gatgccaaga ccatgtattt cgattcctcg ttctttgagt gccatcgctt ctccgatggt
    17461 tgcgataaac gtactgtcga ttcttctgga tagattgttt tggtcaggtg catcatagat
    17521 tcttgagatt agagaagtat ttcccggccg atcttgcgta ttagaaaact gagttccgta
    17581 ataattgctg cttgattgtg cgtgtacccg ctgtacttta taggaaaatg ttggtacacc
    17641 atccgtcagc aaaacgatca cttttttatg tccatttgga acggatagca tgcttcctgc
    17701 atctcttagt gctttttgtg taaaagtacc accatttgtc cgtgaaggtg taatggattt
    17761 tacttgattt ttcactgaat caaatgaacc catctgtact gcaccgttac tgtagctata
    17821 tccttcgctt gagtagccga catatcccat attgattttg tctgtgatac cgctatctgc
    17881 taaagtatcg acaaaacgat cgacaccaat ctttacttca ccgatccgat tattgtcgtt
    17941 catacttcct gaccagtcta cgaccaatac gagatcaaga ggcgtgatat ctttttgtac
    18001 atttccacga gcattcaaat aaacatcaaa caatccgggt gtacttgttt cctttgcata
    18061 ctttcgtatc gcatagtctg cttgattacc ggttcctcca tattcgatat acgaatgggt
    18121 ccgatcatta ggatctccat tccaagaatt tataccatcc cattgatttg ttccgttttt
    18181 attgccttga tgatcaagaa catttgtatt tcctgtaggt tgccaactgg ctttaggata
    18241 agtgccctga tcatctgttg tatattctgt aggaatcgtt tgattagatg gcacactcac
    18301 agctggcaag gataatgtag gcaatgtaaa tgggctgaca gaattttcgt tttcttttga
    18361 tgattcttct tctatttcgt ctggctttgg tactgacaca tctgtatctt tcttattttc
    18421 tgtctcatca ataatgtcat attctttatt atcgacgatc agcttgtttg cctggctgtg
    18481 gtaaagtgct ggatcgattg gaaaactcag atcgatagtt tgtttagtgt ttgatgagat
    18541 ttccaacgta attttcccgc tatttacctg ataagcatag ccatctcctt cttttctttc
    18601 cacatcactt aagtaatttt cgactagacc aatttctttt tctatctttt cagcactggt
    18661 gttattcgca tttagcttga tgttcagtgc tttttttcca gactgatccg aaatgacttc
    18721 agctgaaatt gtttctaaaa ctgttggatc atctgtagtg tctgctattg cagataatgg
    18781 cacaaagttt gataaactca atattatcag catgaaaata actttcagtt tcttccctgt
    18841 ggtcgtcatt tttcccctcc tttttctttt tttctgtttt gacgtgaata gtgaaaatta
    18901 ttaaaataaa caggcgttac ataattttat ttattttaat atgataatta ttatacgaag
    18961 ggcgctaaag ggaatcacac caattttcat accaattggt ttcatttatg aagttgattc
    19021 cttcgattaa tgagcgtttc attgaaagaa aagggttgag ggagaaaaac atatgatcag
    19081 gttgttggaa ttaaactata gaaagatgga aaaaatcatt cgaattctgt ccatttctga
    19141 caagtggatg actaaaaaag aacttgccaa acgaattggt agttcagaat ctacatttgt
    19201 aaggtatatc gaagagatca aaatccgctg gggaacggat gtaacgattc aatcttcaca
    19261 aaaattgggg tatcgtcttg aacatttgaa tgtaccagct tatttaaatg ttctgatgga
    19321 aatgtcgcaa agttctacaa acagtcgttt attaaatgag attattgtca atccgggtaa
    19381 aacgattgag tattattgtg aaaaaatttc catcagccgg tcttctttta cacgtaagtt
    19441 gaaacaatgc aatcaaattt tagaaagcta tttattaaaa gtgatcgttg accaaggttt
    19501 tcagttaact agccttaaaa gtgagctcca gttgcgtatc tttgttacct ttttcttttt
    19561 aagttactac ggaagacgca cgttgccatt tccaatcgaa aaaaagaaga tcaaagaaat
    19621 tatggtcaag aataattgtc agatgaattt gtttcaggaa gaaagcccat atgagcaatc
    19681 tttctttatt atgtattttg ttgtgcatct catgagagaa tcccaaggaa ctccatttga
    19741 atggaaagct ggagactctt atgtacagtt gataaatatc gataaaaaag acttcttaat
    19801 cttgaaaaat ctcttaggaa aattgaagta cagcagctat ataaaagtag tgaactattt
    19861 tgtcaatacg attttttcag aattttcgga gaaagaaaaa aatgaaatca gtgagcagat
    19921 acgaaaaaat atgtcctact gttcgttttc atccgctgat tgcaaagaaa aaaacgaaga
    19981 atatgttgtt caattattaa cgcaaattta ttttttctcc cgtattttcc catacgatac
    20041 gactcatttg acgatccgtg caacttactt tgcacagcag atgcaacacc gccagatacg
    20101 tctatatgct ggatttcgtg cagaacttca attttattcc actctactga atcagaatct
    20161 gatttattat ttatctagct atatcttttg gatgcttatc ggtaatcctg aaattcacca
    20221 ttatacttct gataaaaaaa tactgattat cagcgactta agtcttcgac atagtcaata
    20281 tattgaagaa tatatcagtg atattcttgc tgtgcataaa attcactctg agacaactgc
    20341 tattactgaa gatcagctgt cgaaatacaa tcttctcgag tatgatctga tcgttacgaa
    20401 tcaacccatt ttgaatgccc atgttcctca tatattgatt gatgatagtg tgtcattcgc
    20461 aaatgaagaa gaactcatcc gcttattcga gttatgaata atcaaaaaac agctatgtat
    20521 aaaccaccct gataaaaatt tcaggacaaa tacacatagc tgtttctttt tattgtattc
    20581 cttcagcaat ccaacgagat ttatagcgaa agtcttttaa caactcttct tttgtccaat
    20641 tatttgcaat gagatccgtc aaagtgttca ttacgagggt catggaaaag tataggaaac
    20701 taactgcttc ttccgacgat tgaatggaga aatgattgat tcttaataat tccagccatt
    20761 tttcgttcat tggagaatca gccgatagtt ctctacgttt ggttgtctgc tgatcatttc
    20821 gctgggttaa caagtataac ctttgccaat agatactttc gtgatctaag tcgcagcacc
    20881 actctaaata tttttcgatc cccaaaaaga aatgttgttt gttttcgcta atgaatttta
    20941 agatatcttg atggactatg tgagaactgc ttttgatcgt atacaaataa gcatcttcca
    21001 aatcttgaaa atatttataa aaagcaccgc gcgacatccc catcttgtca acgatttctg
    21061 agactttgac ttggctgatg tgctgtgagc aaaatatctc cagtaatagg tcatcgattc
    21121 tctgtttctt tgcgttcgat aaattgaaaa aagtttcttt tggcatccac atctctcctt
    21181 tatctctgat tatacgtgga tagcccttca ttgtcatgaa ggattcattc cttctttttc
    21241 tcttcttctg tcaagtaccc atcttccatt cgataaacgc gatcactcca ttgaatcata
    21301 cggacatcat gagtaaccat aatagtcgct ttttgtttct catgagcttc tttaactagt
    21361 aatttgacga cttcatatgc atgatctgta tccaaactag ctgttggttc gtctgctaag
    21421 atcaagcttg gctcattata taaagcgcga gcaattgcca ctctctgtct ttctccacca
    21481 gaaaggtctt taggataact atttttaagt gactggatat ctaaagattg cagcaaggaa
    21541 tcgatttttg ttgaatcttc tttttttgaa gtaacttggt caactagttc aaattgttcc
    21601 tttaccgtta aaaaaggaat caaattagag ctttggagaa taaatcctat ttctttaaag
    21661 cgtaaatctg ctcgtttttt ttctggaagc tcagttagtg agtgaccgtt gatcactatt
    21721 tctccggaag tcggtgtttg caatcctcct gaaatggtaa ggaaagtact tttacctgaa
    21781 ccagaaggac caatgatgct gacgaattct ccttccttta cataaaaatc aattgctttc
    21841 aaagctgaga cctttgtatg accagaacca aatgttttgg taactttttt catcgttaat
    21901 atttttttca tttttattaa cctcctattg ctgtaattgg gtctactttg gttatcgtac
    21961 gtatcgaaaa caatccgcct agcattgcga caatgatcaa aatcccacta tatagcaacc
    22021 attgtgacca aaaaacagca aaaggcatcg cgcttggtaa aatcagacta gtcaaataag
    22081 caaacaaaag agcaagcaga acgcctacca ccccaacaat aaaggactgt gctactaaag
    22141 atttagcgat gaagaaattt ttgatccctt gtgctttcat tactccaaaa atagcagtct
    22201 tctgaagtgt catcacgtac atgaaaatac cgaccacggc agcaaccact aagaacaaaa
    22261 agtatatcat cgcatttaag gtcatatttt gggcagaata gccaggaata ctttcaatga
    22321 atgtcccaat agttaataac tgtagaccct tcgatgcttt ttctttcgaa attttagctg
    22381 aatcctttat tacgatccca ttgatcggct gttcttcttc agaagcaaac ggctgttgtc
    22441 catacttcaa agcagtccac gtatctaggg acgcatagat gacgggagaa actgtgtaat
    22501 aagtttcagg aaagataccg acaatcgtca aaggatcgtc gtaactgcca attttgatcg
    22561 tatctccaat tttatagcct gaatcagcca gattttgaga gatgatgatt tcattctttg
    22621 ttttaaattc atgccctttc gtgagttttg gtaataaaaa tgcttctttc gttgtgccga
    22681 aaactgtaac attttctttg ttttttcctt tgattgctcc gctgtacaat ccgattggtg
    22741 ctttctctcc tccttcaatg tatttcagat ctcctcttgt taattgtgaa gctgcaaata
    22801 cttgatttgc atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt
    22861 taaactcttc tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca tacgcaatca
    22921 agaacataat accgacgatc aagccatatc gtaatttaga atagcgaatt tctttgatag
    22981 ctagaaacat ttgtttttcc tccctatgat agattgatag tgacaaaatg tcacttgttg
    23041 aatagtgtaa ctctaaagtg acaatttgtc acttgtgttg tttttttctt atcaataaat
    23101 tgtctaagac atctttcgat aaaaccttaa catgagtcat aaaaaattta tttaagcctt
    23161 atttatgctt tccatgataa actaaagtga ccaaatcaat gaggtgggaa aatgattaaa
    23221 ctattagtag tagaagacga taaagtatta tccaataata taaaagaaat actcgctgca
    23281 atcggcgaga taacacaagt ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga
    23341 gtttatgatc ttgcaatctt ggatttgatg atgccggaaa tgaatggata tgaagtgtta
    23401 gcaaaaatac ggaaagaaaa aatacagact ccagtattga ttttgactgc aaaagatagt
    23461 ttggaagata agattgaagg atttgaacga ggtgcagatg attatctaac aaaacctttc
    23521 tatcgagaag aactgatcat gcgcgtgaaa gcattattga aacgctcgct aggtctcttt
    23581 gatgaaaatg ttctgaagta taaggatatc agttgtgatt taaaaagcaa tgaagtcttg
    23641 tataaagaac aagttttgcc catacaagga aaagagtttg aattgctcgt ttatttttta
    23701 caaaataagg gaatcatttt gacaaaagag cagatatttg accggatctg gggatttgat
    23761 tctgatacga cgattacagt tattgaagtt tatatgagca acttacgaaa acatctgcga
    23821 agtactggat tggataaaga aatcaaaacc ttacgtaatg tcggttatat actgcaaagg
    23881 gtgtgatgca atgaatggag atattacacg aaagcaacgt agtcgctttt ttgcgacaaa
    23941 tgtagcagca tttgctttta tcttttttct tttgggattc attactttac agatattaaa
    24001 ccaatctgca tacagagaaa cagatctgtc attagagaaa ataaattttg attctcgaac
    24061 agtacaatta gagatacaaa gatatcagca aggcgatccc tttttaaacc cgactcaaac
    24121 ggaacaaaac agaacaaaca tggaacagcc attaatggaa ccatcaaatg accgttttaa
    24181 cactcaagtg atcttgtggt ctaaaaatgg tgagatattg aataaagcag ctattggtgg
    24241 gcgtttcaac caaatcaaag aattaaaaat ggatacgaaa aatctgaata ccatccaaaa
    24301 tattgaatta aatgaagaca gtggctcatc tgccctctct ttccgttcga ttacgaaaaa
    24361 agcggagacg aatcaagatg gcgttgccta catccaaatt ttagaaaata caaatcaaat
    24421 caagaattca ttagcgactt ttcgcacgat tatgattttg tgtatgattg ttttttggat
    24481 tctctctatt ggtatcagtt attacttgtc tagtctaagc atgcgcccta ttattctttc
    24541 ttggagaaga caaaaagaat ttgttgagaa tgcttctcat gaattgcgca caccgttaac
    24601 gattattcaa aatagtttgg aacatctttt taccaagcct aatcatacta ttattgaaga
    24661 atccgaaagc atcgctcaag cattgtcaga gacgaggcgc ctcactggat tgacttcgga
    24721 tttgctgaca atcgctcgga atgattctga tcagcagctg ctttcaaagc aaatgatcaa
    24781 tacacaagaa tatatcgaac aacttgttaa accatttcaa gagatggcca tcatagatgg
    24841 aaaagagttt attctccata acgatgcaac attagaagta ccaattgatg aaaaaaaaat
    24901 ccatcaagtt ttggttattt tgctggataa tgcattaaaa tatacgaatc cagaagataa
    24961 gatcattttg gattcatctg ttaaccataa agaatggctg attcacgtaa agaatactgg
    25021 cccttctatc agtgaagaag atcaagcaag gatttttgat cgattttatc gggaagatca
    25081 atctcgggcg aaagagactg gcggatacgg gcttggcttg gcaattgcaa aacaggtaat
    25141 cgaacagcat catggacaga tttctgtatc caattatctt ccacaaggag tcgattttaa
    25201 agtccaattg ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa
    25261 aaatcttgtc acagcccttt tttgttgcga ataaacggtg ttccagaagt agcttcttcg
    25321 gaaataagcc gaaatttctt taagctgacc acttctgtca caacctcttt agcttacgct
    25381 tacgattttt tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct
    25441 tctcggctaa catcttctgt cactttttca acatcttcat gttcttcttt atgaatccca
    25501 acttcttcac gaataactgt atgtttgctt acatctactt gctcttcttt gattgggata
    25561 gtaatcgtgt catcttccaa tttttcgtct gttccctgtg aagattgatc tgttactggt
    25621 ttgcgttcga tgacgatttc ttcacgtttg accggtactt ctaccgtttc agtatcgttg
    25681 accacatgtt tgtggatgtc gacttctcca gttgtcacat catgtgtatt tacatctaat
    25741 ttttcttctt ttagttgaat tgtatcatca tctagatttg gtttgcgatc gatatcttca
    25801 gtcttgcttg tacccattgt atcagtctcg ccaggtacgt ccatcggatc agttcctggt
    25861 gtcatattca atggatccgt tgtattttga aagcctgtcg tgccacctac gtccattgga
    25921 tctgttccag tgccagtttc taatccagtc cccattgtgc ttgctgggct aacagtatct
    25981 tgtgtatcca ttgtatcttc tgttgtttct ggctgacgat agccttttac tgtgatgaca
    26041 aattttccat cagaaatatc tcctctatat tgatataaag gatcattttc tggtgtagcc
    26101 gtctcagaat cataggtatc aaaagaaaaa gtatctttta ttttttccca taaagaacgg
    26161 tcttcttcac catccatccg attttcatct gtttcaacat ctataccaga caatccgtca
    26221 aatcctagtc tagatgctgc ttcatcagta gtataaataa cgatatcatc tcgttcatac
    26281 ccttcgttac gcaaccgttc aattactgat aatgcttctt ccctcgtact atagcttccg
    26341 atgaccgtta aatttttctc ttccatgtaa attcctccta gcaaatagat ttatgattgg
    26401 tatattgtta cttataaatt tattctatag gagacgattg ttttctacaa atattt
//
LOCUS       DAXWLF010000021        24271 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXWLF010000021 DAXWLF010000000
VERSION     DAXWLF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.021"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..572
                     /locus_tag="ACGE4U_000655"
     CDS             <1..572
                     /locus_tag="ACGE4U_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFF8392842.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="ACGE4U_000656"
     CDS             576..1217
                     /locus_tag="ACGE4U_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8392843.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="ACGE4U_000657"
     CDS             1214..2131
                     /locus_tag="ACGE4U_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8392844.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="ACGE4U_000658"
     CDS             2128..2799
                     /locus_tag="ACGE4U_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8392845.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="ACGE4U_000659"
     CDS             complement(3340..3828)
                     /locus_tag="ACGE4U_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFF8392846.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="ACGE4U_000660"
     CDS             complement(4029..4295)
                     /locus_tag="ACGE4U_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8392847.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="ACGE4U_000661"
     CDS             complement(4295..4558)
                     /locus_tag="ACGE4U_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8392848.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5079..5219
                     /locus_tag="ACGE4U_000662"
                     /pseudo
     CDS             5079..5219
                     /locus_tag="ACGE4U_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5361..7184)
                     /locus_tag="ACGE4U_000663"
     CDS             complement(5361..7184)
                     /locus_tag="ACGE4U_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8392849.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="ACGE4U_000664"
     CDS             complement(7782..10487)
                     /locus_tag="ACGE4U_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFF8392850.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="ACGE4U_000665"
     CDS             complement(10793..11740)
                     /locus_tag="ACGE4U_000665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFF8392851.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="ACGE4U_000666"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="ACGE4U_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFF8392852.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLHKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="ACGE4U_000667"
     CDS             complement(12375..12737)
                     /locus_tag="ACGE4U_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8392853.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /gene="alr"
                     /locus_tag="ACGE4U_000668"
     CDS             complement(12748..13869)
                     /gene="alr"
                     /locus_tag="ACGE4U_000668"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFF8392854.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="ACGE4U_000669"
     CDS             complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="ACGE4U_000669"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFF8392855.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="ACGE4U_000670"
     CDS             complement(14365..15876)
                     /locus_tag="ACGE4U_000670"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8392856.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /gene="murF"
                     /locus_tag="ACGE4U_000671"
     CDS             complement(16198..17550)
                     /gene="murF"
                     /locus_tag="ACGE4U_000671"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFF8392857.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="ACGE4U_000672"
     CDS             complement(17703..18779)
                     /locus_tag="ACGE4U_000672"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFF8392858.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="ACGE4U_000673"
     CDS             18975..20720
                     /locus_tag="ACGE4U_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFF8392859.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="ACGE4U_000674"
     CDS             complement(21000..21437)
                     /locus_tag="ACGE4U_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFF8392860.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="ACGE4U_000675"
     CDS             complement(21421..22794)
                     /locus_tag="ACGE4U_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392861.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="ACGE4U_000676"
     CDS             complement(23116..23604)
                     /locus_tag="ACGE4U_000676"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8392862.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="ACGE4U_000677"
     CDS             complement(23626..24228)
                     /locus_tag="ACGE4U_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFF8392863.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6960 a         5163 c         4487 g         7661 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcat aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DAXWLF010000022        24177 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXWLF010000022 DAXWLF010000000
VERSION     DAXWLF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.022"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..1435)
                     /locus_tag="ACGE4U_000678"
     CDS             complement(<1..1435)
                     /locus_tag="ACGE4U_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392864.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDK"
     gene            1557..2141
                     /locus_tag="ACGE4U_000679"
     CDS             1557..2141
                     /locus_tag="ACGE4U_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392865.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2152..2397
                     /locus_tag="ACGE4U_000680"
     CDS             2152..2397
                     /locus_tag="ACGE4U_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392866.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2403..3125
                     /locus_tag="ACGE4U_000681"
     CDS             2403..3125
                     /locus_tag="ACGE4U_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392867.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3256..3999
                     /locus_tag="ACGE4U_000682"
     CDS             3256..3999
                     /locus_tag="ACGE4U_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392868.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4005..4370
                     /locus_tag="ACGE4U_000683"
     CDS             4005..4370
                     /locus_tag="ACGE4U_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392869.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            4386..4916
                     /locus_tag="ACGE4U_000684"
     CDS             4386..4916
                     /locus_tag="ACGE4U_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392870.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5132..5386
                     /locus_tag="ACGE4U_000685"
     CDS             5132..5386
                     /locus_tag="ACGE4U_000685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392871.1"
                     /translation="MMIGMRIQREQSYFEVSANNVIERCHYGQHFWTGEVRENIDREY
                     VQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV"
     gene            5493..5801
                     /locus_tag="ACGE4U_000686"
     CDS             5493..5801
                     /locus_tag="ACGE4U_000686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392872.1"
                     /translation="MYESEFINLTFLNSVFIDYVITTKRIGSYSSTYASLVPMLNSMS
                     RFLKEREKKELQYIKEIMPDFEMHEWQIALSDWKLANKVRRITPFQAKRFLRAMKFVQ
                     "
     gene            5938..6738
                     /locus_tag="ACGE4U_000687"
     CDS             5938..6738
                     /locus_tag="ACGE4U_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392873.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            6775..6957
                     /locus_tag="ACGE4U_000688"
     CDS             6775..6957
                     /locus_tag="ACGE4U_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392874.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7068..7385
                     /locus_tag="ACGE4U_000689"
     CDS             7068..7385
                     /locus_tag="ACGE4U_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392875.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            7388..7531
                     /locus_tag="ACGE4U_000690"
     CDS             7388..7531
                     /locus_tag="ACGE4U_000690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392876.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIVQMFRLPVSE
                     LKK"
     gene            complement(7735..8214)
                     /locus_tag="ACGE4U_000691"
     CDS             complement(7735..8214)
                     /locus_tag="ACGE4U_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392877.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            8298..9671
                     /locus_tag="ACGE4U_000692"
     CDS             8298..9671
                     /locus_tag="ACGE4U_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFF8392878.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            9694..10221
                     /locus_tag="ACGE4U_000693"
     CDS             9694..10221
                     /locus_tag="ACGE4U_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392879.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10208..10771
                     /locus_tag="ACGE4U_000694"
     CDS             10208..10771
                     /locus_tag="ACGE4U_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392880.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            11193..11930
                     /locus_tag="ACGE4U_000695"
     CDS             11193..11930
                     /locus_tag="ACGE4U_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392881.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(11947..12363)
                     /locus_tag="ACGE4U_000696"
     CDS             complement(11947..12363)
                     /locus_tag="ACGE4U_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392882.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKYKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            12543..12758
                     /locus_tag="ACGE4U_000697"
     CDS             12543..12758
                     /locus_tag="ACGE4U_000697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392883.1"
                     /translation="MNLDKYNLYTAREASLILGKGRNYVTNIINYYPNRLPKGSFKKV
                     GDKTYIITEEGIEALRSQTENKNKKKE"
     gene            12755..14002
                     /locus_tag="ACGE4U_000698"
     CDS             12755..14002
                     /locus_tag="ACGE4U_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8392884.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            14270..16660
                     /locus_tag="ACGE4U_000699"
     CDS             14270..16660
                     /locus_tag="ACGE4U_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8392885.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            16709..18544
                     /locus_tag="ACGE4U_000700"
     CDS             16709..18544
                     /locus_tag="ACGE4U_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8392886.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            18619..19476
                     /locus_tag="ACGE4U_000701"
     CDS             18619..19476
                     /locus_tag="ACGE4U_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8392887.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            19466..20575
                     /locus_tag="ACGE4U_000702"
     CDS             19466..20575
                     /locus_tag="ACGE4U_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8392888.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            20914..21714
                     /locus_tag="ACGE4U_000703"
     CDS             20914..21714
                     /locus_tag="ACGE4U_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392889.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            21820..22608
                     /locus_tag="ACGE4U_000704"
     CDS             21820..22608
                     /locus_tag="ACGE4U_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392890.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(22625..24070)
                     /locus_tag="ACGE4U_000705"
     CDS             complement(22625..24070)
                     /locus_tag="ACGE4U_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFF8392891.1"
                     /translation="MLGVILMMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFD
                     FIYPIVESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYS
                     FEDPIPHFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANA
                     NKHKFTKEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESG
                     YYVKGEREKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRV
                     VADAGYKTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPL
                     LFSTITRDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTA
                     LNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLW
                     KTKKPMAFYFKIMQRLTKRSGLITNSFLSTI"
BASE COUNT         8892 a         3401 c         4692 g         7192 t
ORIGIN      
        1 ctttatcctt taattgaaaa ccctgatcca tagtcactgc atttggatat agaagcccac
       61 tataatgttt acgtaaattc acttggaatt tctgattata aaagtctaac agattagaaa
      121 taaaaggcgg aaaattactg tttttgtaaa attgattatt tccatttagg ttgtatagta
      181 atttgttaaa ttgctcctga tttaatttat tgtaaatcca tggtaatact tccatatcct
      241 tatctgctaa cctgaatgaa ctagcatcgg taagatgttc gatcataaaa tctatgaaca
      301 tctctttttc accaaaatat aaatattgat tatatattgt ttctaatgtt gaagatggta
      361 gcgaagaaac tctcattttt tgctggagta taggatcaac tagactattt aagtgatttt
      421 gtaattcttc attgttgttt cctagaactg caagggaacg taaacggtag taatattcta
      481 tttctgttag tacttgttgt ttcggtttaa aatcatttat tgatatttgt aaattttgaa
      541 ttgtctcgtc tgaaaaatca tgttgtaatt ctggatgggt agctataaaa gtaagtaaaa
      601 agaacccttt tgtatacgtg atctgttgat tttcaccaag tgatttatta atttcctctc
      661 ttgattcagc tgtgaaccta tactttgcaa tttctttttt tagaatattt aaatagaatt
      721 ctttattatc aaaaattagg gcattaagta gtctgaaagt actttcccta tctctatctg
      781 cttcctcatt tccataagat aattgaaaaa caaacttaaa ggctgtctta cataggtttt
      841 ctttggcttt ttcgcttagt ttatttagat agctattttt tagtgcagag tgaaattctt
      901 caggtgtaac agacatagca tgtatgcgac ttgcatattg tataatttcg atcgttactt
      961 tttttcctaa tattggattt tttaggaata taatttcata ggacttccgt ataacatcct
     1021 gacaagtttc ttttgttata tctcttaatt cccaagtatt accgttgtaa gtgactacag
     1081 gatgagctga ataatttctt tgatccttaa tatattttaa atcctcatat tcatagctat
     1141 caattagctc agttgaacta taagcttttt ctagtagttc catttcccat tttggagagt
     1201 ttggatcact ttttcttagt ctagctatat cttctaaaat tttcttagaa gtattatcct
     1261 cgtaaatatt tgctaaatca gataatttat caattaaatc taaaataagc gcactgtaat
     1321 tgacagctat tgcagaacga taatttccct gataaaaact acttctaact tcttctaata
     1381 atggaataat tctaggattt ttaacttttt ttatttcatc atctaatata aacatataaa
     1441 tctccttttt ttatttgtat gacaagttta ccataaaaaa aaattaaaaa gcgagacata
     1501 tttatgttgt tgtgttatca taataacata aatatgtaga gtgagatgat gaaaaaatgg
     1561 atttgaaaca atcgttttac atctggttat atcctgataa tcctatgtca ataatggctg
     1621 atatttttgt agttagcatc ttattgtatt tattttatct aattttcttt cacaatttaa
     1681 tcgggtacat gattcttgat tggaaaggaa tgttcgatat agaaagaaaa ccatttgcta
     1741 acaatcaaac ctataccgtc ttcgaaagtg gagattggga agaaaatgaa gttgatgtga
     1801 tccatgaaat tggattagct ttatcaggaa tacaagatgc tgatgttttg gtatcaatga
     1861 caacggatac tggatacaac gcttatgagg tattttatct tatgtatttt atatggcgtg
     1921 atggtcaaga aattatcaat ggtcgtgctg aaaaaacaat caattctaat actgaaattt
     1981 ctttttgtat gggtgaaggg gaaatgattg tatataaaga tgggtcagca aacattaaaa
     2041 cagcctttgg tgatattgaa acaagtatta aggaacttag ggaatatgtg cagaacccat
     2101 ataattttca atatttagca ggaagaataa atttagttta ggagtaaaaa tatgaaaaaa
     2161 attataaatc aaccaacaaa tcacttgata attgaaggtg ttattttcgt aacaatgtat
     2221 tttactgtag gaaatggcat ctcaaataca atcattcaaa aaaatccaca atacctatta
     2281 tttagtcttt atgcttggat tgtacaaatg gtttgggttt ccatattgat gctgttgttt
     2341 tattggatag tacgggttat tcttttattg ttaaacaaaa gaaaaaagga taggtgaata
     2401 aaatgaaatc aactaaattg aaggttgtat tgttatcgac agcagcatta ataaccctta
     2461 cagcatgtgg tacaactaat agtgctaata ataacaaaga cacaaagtct agtagtacga
     2521 tggttactaa aaacagcact acatcggcat caacaacaat gtcttcaatg actgaaaata
     2581 atgctgaaaa gtatcaaaaa tacgaagaca ttaataaagg tcaaaagccg gatgaagttt
     2641 ataacaactt tattaataca gtaaaaaatg atgaaaatta tgggaatgat ccattgcaaa
     2701 ataaaaatct gactatcgtt ctttacaaac ctgattgtcc agattgtcaa aaagcagaat
     2761 caaaaatcat gcaagaaata aaagaaggat ttagttatgc tccaacatta atgcatgata
     2821 ttaccataaa taaaccagct tacgagtatt acgcagttgc tgtagatatt actgatggtg
     2881 tacctgagtg ggtacaaaac attcctggtc tggctgatga gaacaatcaa tatcatacgc
     2941 caacagttgc agtagtaaca ccaaagaaag ttaacggcac actatactgg gatgtaatgg
     3001 gaatgacaac aaatacaact gatgaaatga ttgaaagtac aactaaatat tatatggatt
     3061 tgggagcata taaattatat caacaatcag agcctgttat ggacgatcaa tccaaagtta
     3121 aatagatctt tagggcgtaa gccctatttt tttaaaatta agtgataaca aataaatgat
     3181 ttctgaaaca aacgttctta ctcaactact cgaaacacac aagctaatta actagattat
     3241 aaaggagaat gcgtaatgaa agaaatcagt gcaaatgaag ctcatattcc gcaaaagaat
     3301 caggttggta tatataaggt gcttcatggt gaaggaattt ataaaataca gttacttacc
     3361 ggttacaata atggtgcaaa tgggaagtgg ttaaaggcaa caagtattcg tgaacttaga
     3421 agtgaagtag aaaaaattag gcttggtctc acaagtgaag aaatagctaa actgaaggga
     3481 aataattctt ttgtaattga aaaagaaaag gaaaaacatc tactttatcg tgagctgaaa
     3541 attgaagaat actatgaaat tgatttacct gaaaaattaa gaattattga tcaacgttca
     3601 gaagaaaaat ggaaaactga aatatatcca agcttaaatg actacaagac atcaggaaag
     3661 cccatgtata actacacagg ctttttaacc tacacacaat acaaatatgc ctttcgcaaa
     3721 ttaaaaggca ttaaacaagc tgaaattgcc ctgtatgaga tgaaaaaagt ggatagattg
     3781 aaattgattc aagatttagt gcaaaataca tggaatcaag cgattgtcga gctagaaaaa
     3841 gaaaatggga aaattttaac taataaatat ctgatcgtaa atgaaattga agaattaatt
     3901 ggtttatttt catgtgaaaa aggtgaagtc ttcttgaagt catcaccaac ctattcaatt
     3961 ttggacagtc cgtatcagtt tatacaggca aggagatgag ttagatgtct agcttgaaag
     4021 atgactatat ccaagcaaaa caagagtatg aaaaaataca gatgttgaaa tctgagaaag
     4081 tcgaattatt tttaacacaa aaattagcag aggatcatat tgctaactta gatgctaaaa
     4141 ctttagagga atatgatcgg cagtttaatc agatacatca caatatcatt ttgcttgttg
     4201 agcaggcaaa gcgaaggttt gaaaaagcgg aatatgatct attagatcaa ggaattgtat
     4261 gcttggataa gggattgcga agatattatg gtgcagagtt aaaaaagcaa aatgtagata
     4321 gaaatgcact aaaacatgaa atcttagaat tgataatgca acgaatttga agggatagaa
     4381 agcaaatgtc acaaaacatt agtgctgtaa aacaattaat agaagaagat ttagagacca
     4441 cagttcagag attagcggaa cgaatccatt ttgcaaatta tcaaaccatc tctaactggt
     4501 caagcaggaa cagaaatccg gaagaaattc caatgaaatt tttttatcga atcggaaaag
     4561 aatatggtgt tgatccgata aaaattttcg agaagaccat gatttattac tatcatggaa
     4621 cacctaacga agtgttagaa catcttttgg aagaggcaaa agaaaaatca tcaatattag
     4681 agctatcgca aaatgacaaa gttattcaag tgaaatgcgt tgatccaaca ggaaagatta
     4741 ttgtcggttt tgatattgaa agaaaatcca aaatagattt tattacacaa ttaaaaaaga
     4801 taaataattt agatattcta acgcttagac cagatcatga acagttgttt atgagtttta
     4861 aaaaacaatt gaaagaaata caaaaaagat ataacaaggt gtttcctaat gcgtgaaaag
     4921 ctcaaagaaa tcagagtttt ttggataatt aacaaagaat gtatcatctt atatactctt
     4981 ggatttctta gcatgatctt gttttggtgg ttatatttaa aacagtaatt ggagagggtc
     5041 aagaggattt ttatcaagat aaggatgtgg aaactgcatg tggaataata gattaaaaac
     5101 aggattgtta ttaatagtta tttcctgcgc tatgatgatt ggtatgagga tacaaaggga
     5161 acaatcctat tttgaagttt ctgctaacaa tgtaatagaa agatgccatt atggtcaaca
     5221 tttttggact ggggaagtca gagaaaatat agatcgtgaa tatgttcaaa gaattgtttg
     5281 ggatgcatat tctatcaaag attatcctaa aagtttaacg agtagattat tttacagtga
     5341 aaaagataat caaaaactct cagatttgat gatgaaaaaa gtatgactat tattacgaaa
     5401 gagaaacaga gtgctatatt cgaggggaaa aagcctacaa caaaagtgac tattggggtg
     5461 acaaggcgaa gcgattaact gcaaatttta gagtgtatga atcagagttt ataaacttga
     5521 cctttcttaa tagtgttttt attgattatg taattactac gaaacgcatt ggaagttatt
     5581 caagtaccta cgcatcctta gttccaatgt taaactcgat gagccgtttc ttaaaagaaa
     5641 gagaaaagaa agagctacag tatatcaaag aaatcatgcc ggattttgaa atgcacgaat
     5701 ggcaaattgc tttatcagat tggaaattgg cgaataaggt tcgtagaata acaccttttc
     5761 aagccaagcg ctttttgaga gctatgaaat ttgtgcaata aaaaaagcca ccagacaagt
     5821 tgatggagac taacacaatt accaacagtt gtaattgtgt tagcttacaa attaattatg
     5881 gtgtttagtc aatcccaaat tgtgaaatgg caccaataat tggaaaggaa aaatgaaatg
     5941 tctgaagatt tacgagagct gaagttacga aagaagcagt atgatcagta tttttccggt
     6001 ttgttgaata aacatattga agaaggactt gatcatgatt ttggaaaaga tgaatattgt
     6061 ttatattgcg aacgttacaa tgaaaaaatg tctgaatatc tacagctgaa aaaagagata
     6121 cagaaattag agaaaaaagt acatgcttgg aagtataaag attccatgtc tggttatgtt
     6181 gaatctcaaa ataagccaag aaaaaagaag caagcgtatg ttctaaagat ggtaaatggg
     6241 gaagagttaa ttgtattagc gtacagtgaa gaagaggttc gagaactcgt tgaaacacct
     6301 gtcaggaaaa aggttgaaac aataaatgag atagacgaca gccaatacaa tagcatctat
     6361 attgaaacaa gtcatggtgg atataagaaa ttagcaactt tccttagaag ttctaaagtt
     6421 gggataattt ctaaaaaaga cataagtgct gttaccgata gtttttgtac tttatctgtt
     6481 cctaaagctt atttccaata cgatagtaaa agatttgaac gcttagagaa attctgtaga
     6541 ccgtatactg tattgaaaac taaaaaattt aataaaacta aaagagtttc tatacagtta
     6601 ccgactggtc aaatctcatt aggtgaaaaa gactatctga taaaagtagg aaaagagtta
     6661 attataaaag taccggaaca cttatttaaa gaaaattttg agatacgtga aaccttggta
     6721 tggaaagaag aaaaatagtt tggaagacat agaatggtat aaggggaaat tgaaatggca
     6781 gtaaataaaa tattgcaggt actaatcata gacgaatttg gcggaggaga atggtttgat
     6841 gtgcagtcca tagactttga agaagaagtc gtagttatag aaaatattta cggcgaagta
     6901 tattcatatt cgcttgaagg tgagaatatt gaggatttac ggttgattgg tgagtagaaa
     6961 aaaacagaaa tatcaaaatg gagataaaaa ttaatagcat gtaaagatag tcatgcttgg
     7021 gttaaaaatg ttaaaattca aaaaaatgag tcagaaggga taaaagtatg aaaagagagg
     7081 aatacaaaca aagattaaac gaactgcttg aagaggacga aacattaact catggttcgc
     7141 ctgatgaaat tctatacatg attgataata tggtgatctt tggtggatat gaacttggta
     7201 ataggtcagt tgatcataat attttagaat ttgatgatgt atcatgggaa gaaattcttg
     7261 attggggaat tttggcggta cctgaaacta aaacctatat tagtgacact atggttcctt
     7321 tttttgagga attggattat aaaagacttc ccaaaaatga aaatcatata ctgggaggaa
     7381 actaacaatg ttaagtgact tatttatttt actgatctat ctaggtgtaa gtgaaattat
     7441 tgcgtatttt ttgtttgtca ttgctaatta tggtatttcc agtgcaatcg ttcaaatgtt
     7501 tagattacca gtaagtgaac taaaaaaatg atgagattat cagcatatct tttaatgttt
     7561 ttgaatgtgg tcacgttaat ctttgtagta atcggtgtga ctttcgattt aatggaatag
     7621 tgggaaatct ataatggaaa aaagagacac gaatgaacat attttttgaa tcaatattgt
     7681 tgatggtttt tgcgatcagc gcttaccaga aatctaaaaa agagtaatga ttgtttaatc
     7741 atcactcttt ttttgtaggg aaattgaaat atgtggagct tgtattggtt tagttaggat
     7801 tgcttggcta taaggagcta gttctttatc aaaaccatca aagtaggttt tcagtagcga
     7861 tttaactgat cgctcatctt gtttgatttg gataatttca gcatccaaat cggttgtgag
     7921 atcaggagct tttgatgcga ttacctgatt ttttttatct tccaactctc ttaggctgtt
     7981 taacaagttg taaaaagaat cattttgatt gttcattcca acgtgagttg caattttaac
     8041 agaagtttcg tccgtattgt catcaaaaga actcattgga atatcaagta gtaacgagga
     8101 tgtttctttt actgactttt tctttacagc attttttgtt tttccatctt ttttgctctt
     8161 tactcgagat gtttttggag ctagtttagg taaatcactt gttttatcag tcatttatta
     8221 gcttttcctt tctattaatt tatgttatta tattatcata ataacataaa aaagtacact
     8281 ttttgggggc gtagttattg aaaaaatcta agagaaagat tgccacattg cttatgttaa
     8341 tggtgcaatt tacagggatt gcaatgagtg taatcacaat aaatccgcaa gtgatacatg
     8401 ctgatgggat agctacttat gatgcacaat ttaactgtac tactgaaggt agtgggtcag
     8461 gaacagatag cgatggaaac gatactggaa gtggcgctgt aacaggtgat gtagccaaaa
     8521 atattgaaga tatttacaaa tacatgaatg gagaatatgg cttatcaggt gctagtattg
     8581 caggtatttt aggtaactgg aaacaagaat caggtattga tcctcgagca gtagaagggt
     8641 attacggaga tggacagaga gaagttgact tcatcaaaca atttactgat ggtctgatgg
     8701 gtattggcta tggtcaatgg acagctgggc gacatacagc attagttgat tggtctaaga
     8761 aaaataatgg cggttcttgg tggactacag aagcccaatt ggactttatg ttcaaaggtg
     8821 atgggggatt cgtaggaata ttaaaagatt atgctttaaa tgcttctgat gatgtcgtag
     8881 aaaatgcagt aacttttcat aaaaactggg aaatttcagc tgattccgaa tcagctgtaa
     8941 tagctgtacg tggtgaaaat gcaaaagaaa tctggaagta catgaaaagc aagggtatgg
     9001 acggtgcaaa agatgaaagt aagatcaata aaatatccgg aggttcaagt accggagatg
     9061 gatcgaataa ttcatcaagt tcagcatcag gaacagtaga agatgcctgt gcaactacgg
     9121 atgacgctaa ttctggttct ggatcaattg ggaaagaagg aaaaattaac ggtaaaaaag
     9181 gtcaagtaat agcgaatatg acttatgaag aagctttgaa aaaatatggg aaagcgatca
     9241 cgctaccaaa attcaaagaa ccagattggt caacttcacc ttttaacgga atatatcaag
     9301 gtcagtgtac agagttgacg tgggcttata tgagccaatt atacgatggg aatcaaccga
     9361 ctactggaaa tggtggttat gtatgggaaa gctacaaatc ggcaggagca aaaataacga
     9421 acaaaccaat cgtaggttat gggtttagtg cttcggatgg atggttatgg gctgttggag
     9481 atgcagggca tactggtgta gttatagcgg tgtttgaaga tggatcattt ctatgtgcta
     9541 actttaatgt tccaccaacc caagctccaa caaggggagt ttcggtaacg ttgattgatg
     9601 gagtggacgg atcagataat gtgcatttct ttagcggtgt aggaaaagct aaatttgaaa
     9661 tcgaaaaata aattggaatg aaggacgata tagatgaata gtaaagtgaa agatacaatt
     9721 atcatttctt tgctttctgg tatgttatta gcactgcttg ttggaatcct attcttcaat
     9781 aagcagaatc aggacataga agctgaaaaa cacagtgtgc atattgaaga taagctgaca
     9841 ttctttaaag aggttaaacc aaatgaggtg gatggattac ggaaagattt agaaaatgat
     9901 attcaagaaa tgttgaatgg atcagttcaa aataagtttg cagatcaatc aggtgaagaa
     9961 tttttgagaa aactatttat tgtaacaggt aaaccaccaa ttactgataa gagtagtgag
    10021 aaagaaaaaa tcgagtacta taagcctttt acttttaagc taacaaactt tggaattaag
    10081 acatctagta gtgggttgta tgaagcattg accgattttg aagttcaata caatggaaaa
    10141 aaagtgtatg ataccacatt aaccatcaca ctaaataatg ataagaaagt gactgggggt
    10201 gctttttatg caaaatcatg aaatcactac tgctaaaaag gcgatcggaa tactttccat
    10261 aattgtgatc ctttgttgtg tggtactatt tcaaaagaaa aatgcttatg ataataatgt
    10321 caaagaattg gaagacctta aaagtaaaca aatcgaagta gtcaagaata accagaaagt
    10381 gaaagctatg caggaagctc aatacaaaga attgggaaat gctgaaatta aggaacaaag
    10441 taggaaattc tttaatttat tttacaactg gaattcatgg gaaaaatata cgaagaatat
    10501 gaaggaaata caaaaagaat ttcctcagat agtaaagtct agtcctgttg atatttctgg
    10561 cacgatggta ggcactggta atagtccaat atctagctat actgctgatt actatacaac
    10621 aaatcaaaaa ggtaaaatgg ttgaattgat tacacagaca agaaatactc caacaacagc
    10681 aacaacaaca atttggcgtg gagtaacaag cataggagaa aatcaattgt tttatttgga
    10741 ggatttagat agctatgtaa gagcagagta aaataaaaaa agagctaacg gtaccaacgt
    10801 tagctctttg tgctgacgat gataacaaaa cctaccgaaa aaggattgtt tcatcacaaa
    10861 cacaaacaga aaagaattga taaaaaaccc gaacctcaga atttttatta atcaattcat
    10921 ttagattata ataaattgag ctgaacatta caatacgtat atgttgtaaa acgtaaaaaa
    10981 agtcaaaagt aaatactttc ttaaaaagtc aaataaaact attaaagaca atatatatgg
    11041 tttatttgac ataagaaaat ggtctagatg ataattgtaa tatagaattg tagacttaaa
    11101 taaagcataa aatacctttt atgataaggt atagctctta attttaagca gttttagata
    11161 taagattgaa agtaaagtgg gggagaataa taatggcaga tcataaatta gaaaagttat
    11221 tagaagaaac gtggaatcca aaagaattta gtgaattttt tatggagaat tttgaaactg
    11281 acttagcagt gattgttaaa gatgctctta gagagcaagg gtatcctgaa actgcaaact
    11341 atataaacat taattttact ttgtatactg aaaataaagg aacttgggat ttttgggcta
    11401 ctttagcaaa taaggagtta agtgataaat cagacacagg aattcgtaac ttttttgaat
    11461 caaacagaga tgattatatg tatgctaaca atcaaaataa attgaacttt agagtagagt
    11521 ttgatgaaac acctgaagaa tttatagaaa gacaaccgcc taaagaaaat gtagcaaagg
    11581 tattggaaga tcgttggaat tcagatgaaa tagtttcaac aattagcgaa ctaggcggac
    11641 aatatgaacc gctagtagaa gcggttagag aagagctaag actgaataag tttcctgatg
    11701 ttcaaaatat tgatgtttct caaattgaaa taaacgtcaa gatcacgaat aaacttgatt
    11761 atggtagttg ggcagatata gcattagaaa aatacatcta ctcaacgtta aaagagttta
    11821 tcgaaaatag aatggatatc atgtatctac agcatccaca gtatttaaac tttggtgttg
    11881 aaatagctac acctcttgaa gaatggaaaa tggagcaagg gcttgactag cctttgctcc
    11941 atttttttat tttacaaatg aatacagttc gtcgttttta ccaagaatga ttgtttgccc
    12001 aactttgaga ttgtcaggtg atccttcagc ggtttgtaag gagaacgttc cattgaactt
    12061 atcggatacc gttataggta gctttaattc tgatccttcc tcatctttga gaattgctgt
    12121 ttttcgttca tcaaaataaa cagtctttat taaataattt ttcgtagtga tctcagcaga
    12181 tttgtatttt tttgtagtag taccagtttc tttagaatta gaggtatttt ctacgactat
    12241 tccatctttg gcatttgagc ttgtgtttat ttgattgaat attttcagcg acaagataac
    12301 gctaagaata gtcaatatag aaacaagtgt aattaatact gataatttcc tttttttgtt
    12361 cattttttct taacatcctt ttttgttttg attagttaga tattccttat ctaacaagcc
    12421 tttttaggct attaaatgaa gataaacccc aaaaaatatt gcaattatga cagtgcaatg
    12481 gtactattat tatacaataa attttgcgaa aggggtaggt ctaaatcgca aacaaaaaga
    12541 aattgaacct agacaaatac aacttgtata ccgcaaggga agcaagtttg attttgggta
    12601 aaggtagaaa ttatgttaca aacataatca actattatcc aaatcgtttg cctaaaggtt
    12661 cgttcaagaa agtgggtgac aaaacctata ttataacgga agaaggaata gaagcattgc
    12721 gatcacaaac agaaaataaa aacaaaaaga aggaatgaat atgaagagaa attcgcttgg
    12781 tatggtttta ttggcatcag ctgtacttgc ggggttatca gtaccagtaa cctcacatgc
    12841 tgatgaatgg actgcaaaca caccggatca aatccaaatt acaacaggac aatcaagtta
    12901 tgcgttcaaa aaaggagata ctatttggtc aactgtagag aagcttaaaa gcttaggtta
    12961 caagactact gttgctgagt ttgccgaatt gaacaatgtt gatttggcaa aaggagaaca
    13021 acggactatc ccgattggac gaactttcaa catcagtaaa gatacattaa cagttaagga
    13081 agcagatggc gaagtagtta gtcaaactgt tcaaaaagat aaacctgatc ctattgaaaa
    13141 tacggagcaa gagaacaaaa aatccgagac tacaaaagaa tcggaaccaa cagcaaacac
    13201 ttctgataag acagaaacaa gtgcttcaag tgttgatgga gcaagttcat ctaatacaaa
    13261 taattctaca agtgagaaag aagcatcaca gggaacaagt gaaagtagca ctacggaagc
    13321 tgatactgga acatcaacaa ataactcaca atcaacatca aatgatgcaa atgaatcaac
    13381 cagtacatct tctagtgatc aatcacaaac aggttcgagt gatgaaactg acagttctac
    13441 ttcaccaagt aaacccgaac aaccaactaa tgttgtgcta ggcgttccat ttattagtca
    13501 aggtaacaca atgctttgcg aaggaacatc tttgctagaa gcgttacatt ataaaggtgt
    13561 tgcaacaaat caagatttaa tgacttttgt taaatcaatg ccactatctc ctgacaataa
    13621 tccaaataat ggtttttctg gtgaatggag acacaatgtg gatggaactt atcaaggtat
    13681 gaatccggct cctgtagttt catggggtgt tgctaatggc ggaaatgttg tagatatttc
    13741 tggttcatcg gtacaaggat taaaagatga gattgtaaat ggaaatcctg tagtagcgtg
    13801 ggtaacttat gcctttgaac ctgctcaaca taaaccaatg ttttggggcg atgctatttg
    13861 gaatcgacat gtagtagccg ttgatggatt taaagacggt ttttaccatg ttgtcgatcc
    13921 tgtgtttggg gcaagttgga ttaatgcagg atcatttgaa acagcatata acgtatctca
    13981 attagcggtt tcggtacgtt aataaaagat atcgtttata tactaagtag ttatatagtc
    14041 tttatttgtc cttgcttaac tacttagaaa acttgttgct catggtgggt agaactaaaa
    14101 ggggtgatgc tatgaatact agagcgttga aacagcttgc tttcaacgaa aaaaatgatt
    14161 aataggtgtg gtgattttaa cttaggaatg ggcgcgaagg tcattaccac accgacatat
    14221 cgtggctcat tttcttcgcc taactggtat aaagaaggag taacgagaga tgaagcatga
    14281 aaaaacgata attttaaaat caaaaataat ttattgtttt gcgactttag taatgatgtt
    14341 atctacgctt ataatgcctt tttcagcaat aagagctgaa gcagatgtca caaaaccgac
    14401 aagtgcaaaa tttatagaaa ctggcgtaaa aacagacggt tatattaggg ttaatatgcc
    14461 gaatcatcca aatgaatgga tgatttcaag tcaatttaaa gattctcatg gaaatattgg
    14521 atattgtatg gattcagaat tgccatcccc aacaggaagt ggagccggaa gcttgaagta
    14581 caagggagct ggcagtgatg agttctatcg tatgtttaaa ggtggttttc catcaaaaac
    14641 agcaaaggaa ctgggtgcag gaaatgatac agaagcatgg tatgcaacac aattagtttc
    14701 atgggtttta gccggaaact ttaaagtttc acagattgta tggtcacatc caaaccatac
    14761 cgccgcagaa acagctcgtg taaaaaaagc atttgaaaaa atttatgact atgctaagaa
    14821 tggaaaagat acgcccaaca ctgagttttc tatcacagct tctaagacag ctgacgaagg
    14881 aaaatatcac actttcacat ataaaacagc atcaaataag actggtaatg cgaagctcac
    14941 ttttacatca gcaaaaccag ctggtatgaa gatttatgat gcagatggta aagaaattac
    15001 taacaacact gtgaaattaa attcaagttt tactattaaa gtacctgtga ctacaccaag
    15061 tggtactttg tcatttaaag gtactgctaa tgttagcaca accaatcctt ttacttttga
    15121 tggacgaggt gtgtatcaag atgccgtagt tatgatcact acttcagaaa caaaggactc
    15181 taaatctttg agtgctaaat ggacaagagc tgttggtcag gtaacagtta agaaagttga
    15241 tcaagatgga aatgctttaa aaggagcgga gtttactcta actgacaata acaatgctaa
    15301 atctgttaag acaaccggag atgatgggtt agctacgttt gatttgcaac aaggcttaac
    15361 atacagctta gaagaaacaa aagttcctga aggttatcaa ggttcattta aaaaggatgg
    15421 tattactgtt aataccgatg gacaagaatt tagttacact gctacgaaca caattaaaaa
    15481 aggtaaagta aaagttaaaa aggttgacca agatggaaaa ccattagcta atgctgaatt
    15541 taccttgacg gatgattcat cccaaacaca agcagaaaaa acagatgaaa acggacttgc
    15601 aagcttcgat attgtagcaa acaagaccta cagccttgaa gagaccggag taccaactgg
    15661 ttatacaggc gtattcaagc aagaaggaat taccgttaaa gatgatggac aaacttttga
    15721 atatacagct acaaacactt tgaaaaaagg tttagtaaaa gttcataaag tcgatcaaga
    15781 tggaaaacct ttggcaaatg cagagttcac tttaaccgat gaaaaaggtg caacacaagt
    15841 agaaaaaaca gatgaaaatg gacttgcaag cttcaatatt gtagcaaaca agacgtatag
    15901 cctagaagaa accggagtac cagtaggtta cacaggtgca tttaagcaag agggtatcac
    15961 cgttaaagac gatgggcaaa cttttgaata tacagctaca aataatttga aaaaaggtgt
    16021 agtaaaagtt cataaagtcg atcaagatgg aaaaccttta gcagatgcag aattcacctt
    16081 aacggatgaa aaaggtgtaa aagaagttaa aaagacggat gcaactggtc tggctacgtt
    16141 caacattatt gcgaacaaaa cttataactt agaagaaaca aaagtaccaa caggttacac
    16201 aggcgcattc aaacaagaag gaattaccgt caaagatgat ggacaagtct ttgaatatac
    16261 tgctaaaaac actttgaaaa aaggtgtgat caaagttcat aaagtggatg atcaagggaa
    16321 agcattagca aacgcagaat tcactttgac agacgacaaa ggtgtaaaag ttgtacaaaa
    16381 aacggacagc aatggcttgg caacatttaa tattgttgcc aacaaaacgt acaaaatcga
    16441 agaaacacaa aaccctaatg gttatacagg gaaatggtct aaagaaggca tcaccgttgc
    16501 tgacgatgga caaacctttg aatatacagt aattaatcac cttgtacctg aaacagtaag
    16561 aacaggcgga atcggtacat cagccttcct tcttgtattt ctaattgcag gattgagtat
    16621 ggtgctactg tattatcgag aacagcacaa agcaaaataa aaaagtgtta atggaaaaga
    16681 agtaaatatg aaaaaagaag gctgacgtat gaaatcaaaa aaaatttttt caaatttaat
    16741 gctatttggt actttgctag gagtgggatt gggcgcaagc tcaatctctt ctagccttgg
    16801 actagggggg caagtagcat atgcacaaca aaactataat tatcaaggaa ctggaacgat
    16861 tcatatccat aaagtaacaa atactaatgg ggatgggaac cctgtctcag atattgataa
    16921 caaagggaaa gaaatagctt taccaaatgg tgtaaaacct cttgaaggcg ttgagttctc
    16981 ttatgtgaaa cttactaaag atgaagctaa atccttggga tggacggata gtgatactac
    17041 aaaaggtcaa catattgatg atgctaaagc acgattatta ctaaacaaaa aatacagtga
    17101 agtaagtaga atgaagactg attcacaagg gaatatagac atacctatac aagtaaatac
    17161 agacgattta acagataact tgtatttgat tgttgaaaca ggaacagtca aaggtgtagt
    17221 tgaagaagcg tatcctatga ttgttaacgt acctgaacta aacggatcag atgatcaata
    17281 ctttaattat gatgtacatg tctatcctaa aaactatttc caattaaact cagcagagct
    17341 tgataaaaaa gacgaagatg gaaaggctct tgccggaact gtttttgaat tgcgaaaaga
    17401 tgatggatct gatcaggttg ttggaactta taaaacagac agcacaggta aattaactgt
    17461 agatggtttg gaattaggtg actattattt cctagaaacc gatttgggaa cagatgctaa
    17521 taaaacaacc tatttactag atggtaaaaa attcaaattt agcgttaaac gtggggagac
    17581 tgctaaagta gttcttgatg ttaccaacta caaacgacct gtaattacta aggtaattga
    17641 taaagaaacc gttgatactg gcgaagatgt tcaatataca ttgaaaccat caattcctgg
    17701 taatattggt aattacgaaa aatatgttgt caaagatgtt ctaggtgcag agttaactta
    17761 tgattcatca gaagtgatcg aaggaatgac tttagatact gattacagta tcgaacgtga
    17821 cggacaaacg acaaactatg tctttactga atcaggtatg gataagcttc agaaacaatt
    17881 tgatgcaggg gatcgcttct ttacaatttt gatgaacgct catactaata gtaaagctaa
    17941 aatggataca ccacttaaga atgatgtttc aacgatctac aacaatggat atgaacccga
    18001 agacgaaaca ccagttgatc acgcttatac aatcattggt gatcaccaat tcattaaaac
    18061 agatggagat acaagcaaag cattgagtgg agcaaaattt gtagtgaaga acaaaattga
    18121 tccgaataaa gttacttacc taaaacaaga tccaacaacg aaatctgtta cttggattgc
    18181 agataaatct aaagctactg aattcgtttc cggatcagat ggtaagttcc aaattgaagg
    18241 cttgcaatat acctatgatg tagattttga taaacaaggt aatccaacaa tcacagataa
    18301 aaaaatcaat gattatgctc ttgaagaagt tgttgctcct tcaggatata caccaactag
    18361 aaatgatgtt ccatttgtgg tatcgaatga tgatgcttct agcgaagtaa cagacatcaa
    18421 gaaccttaaa acacctgaaa cagttcgtac aggtggaatt ggaacagtag tatttctagt
    18481 ggttgcaggt gtggcaggaa cagggctata tgttgtctac aaacgagaac aacacaaagc
    18541 ttaatgaaat aaaattccct agagtgtttt tggctctagg gtttcttttt aaaaagtgtg
    18601 tgaaaagtag gtatgagcat gaaaaagaag aatatcaagt tgatcatctt aaaaattggg
    18661 atgttgctat gtttaggagt aggtctatat cctgtaatta ctcaggctta tacctactac
    18721 caaactcaac aaacactaaa ctcctataag gaaaaatatg atgaagcttc tgaaaaggag
    18781 aatgaagaag tcttcaagag atattcagaa gttaatagga aacgaaaaga cacagaacaa
    18841 acctcagtca cgattcctga agataataac aatattccgt caaatggaaa tcttcagaag
    18901 gataaattag gtgaattagt gggtgaaatt cttataccaa agattaatgt ggatttgcca
    18961 atttatgatg gtgtaaacga tttccaacta tcagcaggag caggaatatt aaccggaact
    19021 gattatccaa ctggtgggat tggaaaccat tctgttatat cagctcattc aggcattcct
    19081 aataaaaaat attttacaga tttagagttg ctcaaacttg gtgataaatt ttttattgat
    19141 atttcaggga aaaaacttgc ttatcaagta gatcggataa aaacaattct accaaccgaa
    19201 tttgaggatt tgcttcctgt ggcaggaaaa gattatgtaa cacttatgac atgtacacct
    19261 gttggagata attctcatag gttactcgtt agaggggtta gcatacctta ccaaaaaagt
    19321 attgttgaga aagaaaagaa gaaagaagat ttaaaggtaa aggcgcagat gaactggtat
    19381 cctatcgtag ttgtaggggt tttattaata atagctgtga tagtcattat taaaaaaata
    19441 gtagcaaaga gacgaggaat gaaaaatgaa gaataaaata ctactttttt tgactcttat
    19501 ttgcactttg gttttaacaa tttatcctgc aaatcagctc tatcatcaat tcaaaacaaa
    19561 agagaatgtg gcggaaatcc aagagaaaat tgagaaaaaa caaactcctt caaaagagga
    19621 agtggcaaaa aaagtcgaac aaataaaatc gaacagcggt aaccaaggat tagacaatac
    19681 tcagaaaaca gataaaggag tagacaatac tcaggaaaca gacggtggat catttgatat
    19741 tcgtgaagct gaaggaaagg tcaatccata cacaggagat gttttttcaa aaaatcaaac
    19801 gaaagatttt gaaccatcta cctattcagg agcggttggt tatgtctata ttccaaaaat
    19861 aaatgtgcta gagcttttgt ttctaggcgc ttcacaggag catttatata gaggtgtggc
    19921 acaaatgagt gctactagtc tacctttatc aggtaaaggt catcaaacag taatagcagg
    19981 tcatcgtggc ggttatacgt caccacaatt tctttatgtt gatcaattag tagccggaga
    20041 ccacatttat atagattatc ttggtgaaaa agcagtttat acggttttcc gaacagacgt
    20101 tattagaaat tacggtcaga attcaacact gactgatatt cctgatttag atatgttaac
    20161 actttatact tgtcatccat atcctcagaa ctacgatcgt ttattagtta gaagtctaag
    20221 agatgaaaaa gcaaaataca tcacgccaca agtaacagtt ggcaataccg catacgctga
    20281 tgccattgat agcgagtccg tttcagataa gacagaaact gttacatctt ctaatgattt
    20341 atttgaagat gttgatcgtg ccaacaagga attagatgga aataatgatc ttaatattcc
    20401 ggaacaaggt agcgtagaga tgcaagacag aacgtttgct gaaaaggcaa gttcaatatt
    20461 tacaggaatt accaataaac atcagaaaat gcttttagtc atttggactg taataggagc
    20521 attagcaagt ctaaaggttc ttattctttt aatcaaagag tttcgaaaag cataatgaag
    20581 attataaaaa ttgatagagt acagtaaaaa gggattgata ctattattga tctctttttt
    20641 attttgaata gagctagatt cacttttaaa taaaatcaaa actgatctta attctaaaga
    20701 aaaagtcaag aaaaaaatac aataaatttt tttatcagaa tatttatcag aatattatta
    20761 gaaaagataa actcaaaagc ttaatttaat agggttattg gttgtattta gaaataatgt
    20821 atggaaaagc tataaagcaa tagataagat gtagaaaatc ttatttgtga atgaaaaaca
    20881 agtcatcgta aaatattaat aggaggtgta gtaatggtgg gagtatcaaa aacagcttta
    20941 gactcattag ctaaactatt tagtagcagg tttgatggtg cagaatttgt tgaatatcta
    21001 agccaaaacg aaagtggaaa aactctgatc gaaacaatga aaaaagacta tttgaatgaa
    21061 aatgactttc cgacatggac gaatattgaa gatgctacat gggatgtagt atgtacgata
    21121 tattctcctt gggtaacgat tgctgatttt gttttagata aaattggagg ggatacatac
    21181 cctgatgcca aatctcttgc tacaagtgac tttaaaacgt tctatggtta tctaataggt
    21241 ctaataggca atggaataga tttgaaagtt acgccaaaac aaacacctga agaaattgaa
    21301 aaagaaaaag gagaggttca aatggcttat gcaaatgaca gagaaaaagt aaaattattg
    21361 gaaggtaaat tatctgaaga agaacttgga aaaatggtgc aagaaatcaa tgaagacagc
    21421 aactattttc ctgaattgaa ggtatatcaa aatgacgaag atttctttaa tgaaatgttt
    21481 agtaatagtc catgggaagc agtaagtaaa acaaatttag gagaatggaa ctttcaagat
    21541 gatctagttg catttagcaa tgatgggtat ttacgatcat attcagaaag tgagtataga
    21601 gctgaattaa aaacgtatga aacggatatt ctgatgagct atttaaatct attagagagt
    21661 ggtcagggag agttaagaga taaagttgaa gaactattaa aagaagagct atagggatta
    21721 tttcaacata aacacaagca gaaaactacc aaacaaaacg taccatgcaa aaagtcggtt
    21781 aaaccggctt tttgctctat tatgagagga gatcaaacca tgagtgcaac aaccgatatg
    21841 gctttagaag cagtaataaa agataataca ggaaatgtcg aaaatgtaga agactttagc
    21901 gatattgcaa tcaatctttc agcatggatt tcagctgtag gagaagaaaa tatcgaattt
    21961 gagatcaaga atatggatga cttaaaagca aaatttcctt ttattgcatc gaaactaagt
    22021 caagttgccg aggataaatc gtggaatgaa ttagttagtt tttccgaaaa aatttatgat
    22081 attacgcaag aagatgagtg tgtttcatgg gaaatgaata atcaatacaa gaaaattgac
    22141 gctttcgtca aatcacgtat agaaggaaca gcttttgagt ttgaaggtat agataccgca
    22201 gataattggt taaataaaga atcctttatt aaaagtatag cggatgatct tgaaaaacaa
    22261 tggtctaagg aagactatgc agagtaccta aaaacagaaa gagatacaga agtgaaagaa
    22321 aaactcactg aggtatgttt ggatgaacat attgatccca atatcgtaaa acaagaagac
    22381 tatgaagtgg ttattgataa agttcctact gattttgagg aagttgtaaa gtatatagtt
    22441 gaagaagcat tatcgcaata ctcttcagat gaggaagtgg acgaagaaac tgttcgtacc
    22501 agagtttggc aactggtttt tgatgacaaa gattataaat atttgagtga tcaaaactcg
    22561 gttaattgga gtgtacgatt attaattgat caaaatgata ttgaatagag ctgaaaagct
    22621 ctattcagat tgtagacaaa aaggagttgg taattaagcc actccttttt gttagtcttt
    22681 gcataatttt gaaataaaaa gccataggct tttttgtttt ccatagccat gtggctagtt
    22741 tttttaaatt catggcagca aacgtaagca tcgtgtgcgt ggcgactttt tcaagacctc
    22801 gatatttcgt ccaacgcata ccatgctttt cttttgcatc tgcaaatatt cgttcaatgg
    22861 tttgttttct ctttttgtaa atttctctat tcaacgccgt atggcgttgg tgttcaagtt
    22921 catccataag atcagcccaa atatgtcgag tcactatttt ttgatgtttt gtactcaaag
    22981 tacaattttc aagtaatgga caactcacac agaattttgg atttgattta taggttcgat
    23041 aaccatcacg agtgatagtt gaaaataaca acggttgatt attgggacat aaatagcagt
    23101 caaaataggc atcataaaca aagtcttttt tacgaaacat tcctttcact ccatgaggtg
    23161 cagtatatgg aagaatcggg tagagtttca agtgtgacag aaaatgaaca aatttaggcg
    23221 tcttatatcc tgcatcagca accacacgct ttatatttgg aaaagctctt ttcgactgtt
    23281 taaccagatc gattgcgact tggctgtcat gcacatttcc tggtgtgatt aatgtagata
    23341 agataaaacc gttatcttca caggaagtat gtgcagaata agcaaattgt ttttctcgtt
    23401 cacctttaac atagtaacca ctttctggat ctgaatgact tattttttga agactcaact
    23461 cactttctgt gtcccatgta aaaggacgtt ttcctacagc aatacgttct ttatttattt
    23521 catcttctaa ttcatcttga taagcttttg cttgaccgta ggtcatctct ttcgtgaact
    23581 tatgtttatt ggcatttgct ttgatatgag tagaatcaat ataaaggttg tcttccgtaa
    23641 cgaagccagc ttttacagct tgttctagaa tatgagagaa aatatcttca aataaagttg
    23701 tttctctaaa tcttcgaaca tagtttttac caaaagtaga gaagtgtggg ataggatcct
    23761 cgaatgaata acctaaaaac caacggtaag caacatttgt ttcaacgtct ttaattgtct
    23821 gtctcatcga acggatacca aataaatatt ggataaatac taatttaatt aagaccactg
    23881 gatcaatact gggacgacca taggttgaat acgtagattc tacaatggga taaatgaaat
    23941 caaactgaat cgcagcatca atttttcgaa ctaagtgatc tttaggtaca agatcatcaa
    24001 ttgttaacat agcaaattga tttctacctt caatcgattg ttttctcatc attagaatca
    24061 ctcccagaat agtttataca attagtataa aacaaaaaac tgcaaacaac actccaaatt
    24121 taggagtttg tctacagtct gaataaattt taaatttatt gtccataatg ttattat
//
LOCUS       DAXWLF010000023        24003 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXWLF010000023 DAXWLF010000000
VERSION     DAXWLF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.023"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(214..1509)
                     /gene="purB"
                     /locus_tag="ACGE4U_000706"
     CDS             complement(214..1509)
                     /gene="purB"
                     /locus_tag="ACGE4U_000706"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFF8392892.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1522..2646)
                     /gene="purK"
                     /locus_tag="ACGE4U_000707"
     CDS             complement(1522..2646)
                     /gene="purK"
                     /locus_tag="ACGE4U_000707"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFF8392893.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2639..3127)
                     /gene="purE"
                     /locus_tag="ACGE4U_000708"
     CDS             complement(2639..3127)
                     /gene="purE"
                     /locus_tag="ACGE4U_000708"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFF8392894.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3543..4850)
                     /locus_tag="ACGE4U_000709"
     CDS             complement(3543..4850)
                     /locus_tag="ACGE4U_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFF8392895.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4853..5434)
                     /locus_tag="ACGE4U_000710"
     CDS             complement(4853..5434)
                     /locus_tag="ACGE4U_000710"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFF8392896.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5550..5647)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6006..7298)
                     /locus_tag="ACGE4U_000711"
     CDS             complement(6006..7298)
                     /locus_tag="ACGE4U_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8392897.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7295..7918)
                     /locus_tag="ACGE4U_000712"
     CDS             complement(7295..7918)
                     /locus_tag="ACGE4U_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392898.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7921..8757)
                     /locus_tag="ACGE4U_000713"
     CDS             complement(7921..8757)
                     /locus_tag="ACGE4U_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8392899.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8774..9661)
                     /locus_tag="ACGE4U_000714"
     CDS             complement(8774..9661)
                     /locus_tag="ACGE4U_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8392900.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9677..10960)
                     /locus_tag="ACGE4U_000715"
     CDS             complement(9677..10960)
                     /locus_tag="ACGE4U_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8392901.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRIKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11643..12326)
                     /locus_tag="ACGE4U_000716"
     CDS             complement(11643..12326)
                     /locus_tag="ACGE4U_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFF8392902.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12344..12961)
                     /locus_tag="ACGE4U_000717"
     CDS             complement(12344..12961)
                     /locus_tag="ACGE4U_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFF8392903.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12971..13795)
                     /locus_tag="ACGE4U_000718"
     CDS             complement(12971..13795)
                     /locus_tag="ACGE4U_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFF8392904.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGTKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13788..14348)
                     /locus_tag="ACGE4U_000719"
     CDS             complement(13788..14348)
                     /locus_tag="ACGE4U_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392905.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14522..15478
                     /locus_tag="ACGE4U_000720"
     CDS             14522..15478
                     /locus_tag="ACGE4U_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8392906.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15783..16739)
                     /locus_tag="ACGE4U_000721"
     CDS             complement(15783..16739)
                     /locus_tag="ACGE4U_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFF8392907.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16843..17445
                     /locus_tag="ACGE4U_000722"
     CDS             16843..17445
                     /locus_tag="ACGE4U_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8392908.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17533..19032)
                     /locus_tag="ACGE4U_000723"
     CDS             complement(17533..19032)
                     /locus_tag="ACGE4U_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFF8392909.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19048..19572)
                     /locus_tag="ACGE4U_000724"
     CDS             complement(19048..19572)
                     /locus_tag="ACGE4U_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFF8392910.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19870..20619
                     /locus_tag="ACGE4U_000725"
     CDS             19870..20619
                     /locus_tag="ACGE4U_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFF8392911.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20610..21395
                     /locus_tag="ACGE4U_000726"
     CDS             20610..21395
                     /locus_tag="ACGE4U_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8392912.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21364..22080
                     /locus_tag="ACGE4U_000727"
     CDS             21364..22080
                     /locus_tag="ACGE4U_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8392913.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22182..23201)
                     /locus_tag="ACGE4U_000728"
     CDS             complement(22182..23201)
                     /locus_tag="ACGE4U_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFF8392914.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23220..>24003)
                     /locus_tag="ACGE4U_000729"
     CDS             complement(23220..>24003)
                     /locus_tag="ACGE4U_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFF8392915.1"
                     /translation="VSKPFFLSYKGDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVM
                     YSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQ
                     VSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAAS
                     EKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIVIMMATPAATVAAAYA
                     ISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7073 a         5191 c         4215 g         7524 t
ORIGIN      
        1 ttcttcatcg atcattaaca gacaaagaac tggcccgcca aggatatgag gacatctcct
       61 tgaagtacca gttcgtacac tcaacttatt gaaccgccgt atacgggtcc gtacgtacgg
      121 tggtgtgaga ggggcggaag gtcagttggc tttcccctct actcgattat ttttgctatt
      181 ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc gtttgaatat
      241 cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt cttctttaga
      301 taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca cttggttgtc
      361 ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg tcattccctt
      421 atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg cagacatatt
      481 ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa aacgatttaa
      541 catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg aagaatgaga
      601 aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt gtccgcgcac
      661 cactctggct agaccagcca tattttctga accgatcgga ttccttttgt gcggcatagc
      721 agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt cggatttttg
      781 caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg ccatcgctga
      841 gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt gtggacggat
      901 ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga acgttcccac
      961 tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc gttcgatgtt
     1021 ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg gttcagcatg
     1081 gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt tttcgccaat
     1141 gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct tgatgagata
     1201 gccataagca gtatctacga catccgtact tgttagacca taatggaccc attttctttc
     1261 ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc gtgttaattt
     1321 ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt ttttcacatc
     1381 ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct cgacttcgag
     1441 ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc gtgtataacg
     1501 ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt cttccagcgt
     1561 ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt tttttgcttg
     1621 tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc gatagctttc
     1681 cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac gtactttttc
     1741 tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca tagaacaagc
     1801 ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca catatagccc
     1861 gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca ctgcttctgc
     1921 aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg cggctggagc
     1981 aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa aagtcgtgaa
     2041 atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg ggatccacgc
     2101 ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat ccgcccgact
     2161 tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca aaacacaagg
     2221 atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg ttgcataagg
     2281 agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt cttgcgtaat
     2341 cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat ttaacgcctc
     2401 tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa gctctttcag
     2461 cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg cggctggaca
     2521 atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac taatcgccat
     2581 catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca acaaaggttt
     2641 aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt ctttcagcca
     2701 atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt agtccagcat
     2761 taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca ggcatttgga
     2821 caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt acaccgataa
     2881 ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc cctgctcctg
     2941 caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac atcaaatctg
     3001 gggttcgatg agcagaaacg accttttttt cataagacac accgaattca tccaatattt
     3061 gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca gagatttttg
     3121 ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca ctttttttga
     3181 tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg tttattaaag
     3241 ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat aaacggtgtt
     3301 ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa gctttttttt
     3361 gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc cttcaacggt
     3421 gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga ggaactattt
     3481 ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt tcttttgctc
     3541 tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc attaaagatc
     3601 aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt gaacatttgc
     3661 aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag cccaaatcca
     3721 acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc caacagcata
     3781 cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc aagtaccggc
     3841 tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac aatcaagaag
     3901 aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac caatccgaca
     3961 ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat aacggcaagt
     4021 ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc agtaatgtca
     4081 ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat gatgatcatt
     4141 agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg tggaacatgg
     4201 aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc agctaagatg
     4261 cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt tgccagcatt
     4321 tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt tttgtcacca
     4381 aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg aatcaaagtc
     4441 aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt tttaatttta
     4501 gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga tccgtagata
     4561 gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg gatcgcacat
     4621 cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg caataatgtc
     4681 gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc ttgattaaag
     4741 cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat cgctagcaga
     4801 tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac gattatgcgt
     4861 cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca agagaaacga
     4921 ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt tcgatgacga
     4981 tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat ccttttgccg
     5041 cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa gataaaaatt
     5101 tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca gaagctgtca
     5161 ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt ggaacatcta
     5221 attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc acaactttcg
     5281 tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc atcaatacag
     5341 ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct aatatacgtc
     5401 cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta tcatttatta
     5461 ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag aaaaacaccc
     5521 tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat agtccagcat
     5581 ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta ggtatgtaaa
     5641 actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg cagtttgcca
     5701 tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac tttctgaaac
     5761 tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc attattcctt
     5821 tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca gcccacataa
     5881 aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata gtgttcaaaa
     5941 gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt tccgtaaatc
     6001 tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc tgaagcaata
     6061 gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg agttgctgcc
     6121 cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa acgcggatct
     6181 cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc accgaacatc
     6241 ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc tccacctgca
     6301 ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc attgaaagaa
     6361 acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat cgcaccagtt
     6421 tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga aaatacactg
     6481 cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg caattcctca
     6541 ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc atagcatcga
     6601 ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa gtttaatcca
     6661 ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag atcaaaatga
     6721 tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg ccatcttctg
     6781 aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga aaacacatca
     6841 ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc ataacgataa
     6901 ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat ttcttgagct
     6961 ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt tttcattgca
     7021 cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc agccggccgt
     7081 acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt tttctgtcca
     7141 tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac atcacaaaaa
     7201 ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa aaaacgcgct
     7261 ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc tccttttctt
     7321 ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg agtaaaaaat
     7381 catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga aaccccttga
     7441 cgttttttga caacagccag atattccttt tgatcaaata aacaagacta tctgctttat
     7501 cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa agcaacagac
     7561 acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa taccaatcaa
     7621 ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc cgtttgattt
     7681 ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga taataagaag
     7741 gatacatcat caaaaaataa atcagataga caacacgagc agacagcact aatcctaaag
     7801 tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg gttaaaaaaa
     7861 tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc tttttcatat
     7921 tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga agaataagaa
     7981 aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg cgtaagtcgt
     8041 actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag atctcggctt
     8101 gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact ggatgatgat
     8161 cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga cactatactt
     8221 gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga ccatgaattg
     8281 catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca gcggcacata
     8341 cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa tcacttgtgc
     8401 aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt tgaaatcgat
     8461 tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg tcgaaactgc
     8521 agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa accccgccca
     8581 tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg aaccactcat
     8641 aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat aaatcatgac
     8701 tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat tattcatttc
     8761 ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc agaaaggtga
     8821 cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc atagaattaa
     8881 aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca ttcggaccac
     8941 cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc tgcataacca
     9001 agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac gtctgccatt
     9061 tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct ttgatacctg
     9121 cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca agagggatca
     9181 tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt tccaggaaaa
     9241 tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata gaaatactgc
     9301 caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct ttgatattcc
     9361 tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca gatgcaaata
     9421 catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc atctttatat
     9481 aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg aacgaataat
     9541 aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg aatggagaag
     9601 caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt tcttttttca
     9661 tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga ctctctgctg
     9721 cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg tattggtctt
     9781 gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc atatcggaaa
     9841 tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga gcgagctgtt
     9901 ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca ttggcttctt
     9961 cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct gagctttttg
    10021 ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct gcttttatgc
    10081 gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat atttctgtca
    10141 actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc tgaaaagaca
    10201 gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg tctaccagat
    10261 ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc aagctgttag
    10321 atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt tgaataatac
    10381 agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct tcagtgattt
    10441 gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca gggatattta
    10501 attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg ttagacagtc
    10561 caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca ttagaaatat
    10621 caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg tattgaggaa
    10681 attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt gtacctaatt
    10741 ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg ggattttttt
    10801 cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa ccccaccaag
    10861 ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag aagaataaca
    10921 taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc tctacttcca
    10981 ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac gatctttttc
    11041 aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca actcaatact
    11101 ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact tcttgcttca
    11161 atgatgggag gataattacg caaagaaaaa aggatataag acaactgccc aatatccttg
    11221 acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca gaaaaataac
    11281 tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca ttgactatgg
    11341 caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc tgctactaaa
    11401 ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc gcttcaataa
    11461 atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact cttcttatgt
    11521 acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa caaatagtac
    11581 tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt gattctcacc
    11641 tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg actaaaaagc
    11701 tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat ggtctcagca
    11761 atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata ttttttacga
    11821 aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat cgaaatacca
    11881 gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat aactggaata
    11941 tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt caaaacgacc
    12001 cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc accttgtgtc
    12061 aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat gcttttagta
    12121 atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt tgtattacgt
    12181 atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa tatcttgatc
    12241 ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa ttccgtcatg
    12301 atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc cggtcatact
    12361 gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg gggtctgtcg
    12421 cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc gtaatcactt
    12481 tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta tggtcatcca
    12541 tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc cattcttcgt
    12601 taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc ttgatcccga
    12661 aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct aatatagctt
    12721 gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg gcatgcggaa
    12781 caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga ttatcagtaa
    12841 aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact gctacaccgg
    12901 caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt tttttcgcca
    12961 tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc aattggtgtt
    13021 tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt tcatatgtga
    13081 tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac tgcgcagcat
    13141 tatctgcgat ccatttagtt ccaggctgat ctccgtaact tgcatccaat acagtagaag
    13201 gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg tggtgttcgc
    13261 tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct tctgatacac
    13321 cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg gatgatacag
    13381 accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc ttggcaaatg
    13441 cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat gatgccaatt
    13501 gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa actagcttcc
    13561 aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt tctttgtcct
    13621 ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt ttggtgcttg
    13681 ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta aaaataacat
    13741 agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta ttcacttgaa
    13801 aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt ttcttccaga
    13861 ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt ttgaacatct
    13921 gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg aatacttact
    13981 ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag ctcgactttt
    14041 gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt gtactctgca
    14101 acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt ttgcgttagc
    14161 atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga tggcagattt
    14221 ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta ttcatctaaa
    14281 aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc aatatatact
    14341 tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca aaaagcacct
    14401 cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt attcttcgtg
    14461 atttttgtta taataataga cagacaagaa actcaatcca actatgaagg agcgttttca
    14521 gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg agtttgccga
    14581 aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt cagaaaaagc
    14641 ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc aagaaatgtt
    14701 agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc atatcgacca
    14761 tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca tcacgatgaa
    14821 taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga tcttagcaga
    14881 agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa tggatacagg
    14941 aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag aaccagaccc
    15001 taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata tcggtacgta
    15061 tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg cctctacaat
    15121 ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta acaaagaaaa
    15181 tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg gtatcgttgc
    15241 ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg cggagatctt
    15301 atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag caatgaatta
    15361 cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct ctctcttctt
    15421 gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa atatgtaatt
    15481 ttaaaaggtt gtgaaaaagc tgttctgcat caattaataa gaacagccaa acaaaaatag
    15541 ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt tgaacaccgt
    15601 ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca atttcaaaaa
    15661 acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac aggagaacac
    15721 cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg aattcacaac
    15781 ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa acttttaaaa
    15841 gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact agtcggcaga
    15901 ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg tctgccatca
    15961 tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg ttttccataa
    16021 taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac attggcctgt
    16081 gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca gtagttttct
    16141 tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg ttcgcatttg
    16201 atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct caaaatattt
    16261 cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg atctggcatg
    16321 taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc ttcattgccg
    16381 atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac cgaatttact
    16441 actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa taaaaaaaga
    16501 ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt tgccgtagct
    16561 aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt ggtatttcct
    16621 acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa gctacccgct
    16681 gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc tatacgcatt
    16741 tacttccccc ttttttattt taattatact tgaataatga aaaactagct atcagacaaa
    16801 agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt tggccgtttt
    16861 cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat cttgtttttt
    16921 catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt ttctttgcgt
    16981 caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa tacattaggt
    17041 ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga ttttttggta
    17101 gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga tggtattaac
    17161 aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt gagcatccct
    17221 caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat agggacattc
    17281 attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa ggaagaacta
    17341 tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa tttacaagag
    17401 atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc ttttgcaata
    17461 aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga ttgaacggtg
    17521 aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg gcgcttcata
    17581 catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt ccttcaagtc
    17641 aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg tggcataatt
    17701 tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga caacagagat
    17761 tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta gcatatcgat
    17821 aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga tcttttgttt
    17881 tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt gtccagctaa
    17941 acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt ctgcttcggc
    18001 ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt accgatcggc
    18061 atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga tttctttttc
    18121 ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct gttcgattac
    18181 ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg ctttagcaac
    18241 atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg attctgcgat
    18301 ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag cttttttgat
    18361 acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga cttgggcgat
    18421 tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt tgatagtgaa
    18481 agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt gtacgctttg
    18541 actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa gaatcgagcg
    18601 caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg tcttgcctaa
    18661 aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga tgatcgaagt
    18721 accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg agacgtccaa
    18781 tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga cgaatgcgcc
    18841 acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat ttttgcttcc
    18901 taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt acttcgctac
    18961 aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga taaaaacaac
    19021 tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat aaggcatgac
    19081 ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct cagctgtctg
    19141 ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac ttttcaactg
    19201 aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt gtccttccag
    19261 agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca atatatagaa
    19321 attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata aaaataaaga
    19381 tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag ttaaccaagg
    19441 gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa aaataatcaa
    19501 taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct caagtgcgat
    19561 tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa tgtttgaaaa
    19621 ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt gtgtttatcg
    19681 ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat tctttcgagg
    19741 tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac gatcatcctt
    19801 gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat caggaatttt
    19861 attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc taaaatcgtt
    19921 gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca tgcgattcaa
    19981 ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt catggaccct
    20041 gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt ctcaagtcat
    20101 gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga aggaatgtta
    20161 gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat tggtgcagtt
    20221 tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca aaaaatgatt
    20281 gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt tcttgcccaa
    20341 aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca gttaagtgaa
    20401 aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc tgataaaact
    20461 atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca gctattggat
    20521 ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa gagacgattg
    20581 agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa actatttcta
    20641 gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact ttacgaaatc
    20701 gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat ataaactcca
    20761 tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt ttgattgatt
    20821 tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat tataaaaaag
    20881 agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt ttattaggca
    20941 aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat tcttttatcc
    21001 ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg aacttacttg
    21061 ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc cggattcatt
    21121 taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac ggtgtcggga
    21181 tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag ggaatcaatc
    21241 gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga tttttagaat
    21301 tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa aaggaagatc
    21361 aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga attgaatcgt
    21421 acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa tgtagaagaa
    21481 ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt atacatgcct
    21541 ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga tatcccagtg
    21601 attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg ctacggtgcc
    21661 ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat cgaaaagtat
    21721 cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt gatcgatcaa
    21781 gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt gcctaaaggt
    21841 ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga tactattttt
    21901 tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa gaaatatttg
    21961 aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga aatcggccgg
    22021 ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga ttactgttga
    22081 gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac tgtttacttg
    22141 gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa ttgacgggct
    22201 ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg aaagttctcg
    22261 aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac atgcggggtt
    22321 acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg aatcgtctcc
    22381 catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc ggttgcaaaa
    22441 ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg aagctctcca
    22501 cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc ttcattgatc
    22561 atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc gcttttggct
    22621 aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt ctgatgaata
    22681 tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt caaccctatc
    22741 ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa catttgctca
    22801 tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt tctcaaaagc
    22861 atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga tggtacacgg
    22921 gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac tcccacagtc
    22981 cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt ctcatctgct
    23041 atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat attgtcatgt
    23101 gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt ttcaaaaaaa
    23161 tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc ctccatttct
    23221 tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac aggtgtcagt
    23281 accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc ctccctatca
    23341 aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat gataacgatt
    23401 gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac tgctagaatc
    23461 acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca tgaagttttt
    23521 tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc tagccacgcc
    23581 aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg tgcagtctgg
    23641 tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac tgacacttgt
    23701 ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat aatgattgga
    23761 ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat ttttatgtat
    23821 ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata catcacgccg
    23881 acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga accaaagatc
    23941 gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa aggttttgaa
    24001 act
//
LOCUS       DAXWLF010000024        23869 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXWLF010000024 DAXWLF010000000
VERSION     DAXWLF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.024"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            141..1010
                     /locus_tag="ACGE4U_000730"
     CDS             141..1010
                     /locus_tag="ACGE4U_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8392916.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1219..2244
                     /locus_tag="ACGE4U_000731"
     CDS             1219..2244
                     /locus_tag="ACGE4U_000731"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFF8392917.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2283..3233
                     /gene="manA"
                     /locus_tag="ACGE4U_000732"
     CDS             2283..3233
                     /gene="manA"
                     /locus_tag="ACGE4U_000732"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFF8392918.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3327..5030
                     /locus_tag="ACGE4U_000733"
     CDS             3327..5030
                     /locus_tag="ACGE4U_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8392919.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5032..7065
                     /locus_tag="ACGE4U_000734"
     CDS             5032..7065
                     /locus_tag="ACGE4U_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFF8392920.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7076..7516
                     /locus_tag="ACGE4U_000735"
     CDS             7076..7516
                     /locus_tag="ACGE4U_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8392921.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7716..8843)
                     /locus_tag="ACGE4U_000736"
     CDS             complement(7716..8843)
                     /locus_tag="ACGE4U_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8392922.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8846..9523)
                     /locus_tag="ACGE4U_000737"
     CDS             complement(8846..9523)
                     /locus_tag="ACGE4U_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8392923.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9787..11553
                     /locus_tag="ACGE4U_000738"
     CDS             9787..11553
                     /locus_tag="ACGE4U_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFF8392924.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11568..12005
                     /locus_tag="ACGE4U_000739"
     CDS             11568..12005
                     /locus_tag="ACGE4U_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8392925.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12018..13169
                     /locus_tag="ACGE4U_000740"
     CDS             12018..13169
                     /locus_tag="ACGE4U_000740"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFF8392926.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13231..13929)
                     /locus_tag="ACGE4U_000741"
     CDS             complement(13231..13929)
                     /locus_tag="ACGE4U_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFF8392927.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13922..15274)
                     /locus_tag="ACGE4U_000742"
     CDS             complement(13922..15274)
                     /locus_tag="ACGE4U_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFF8392928.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15497..16897)
                     /locus_tag="ACGE4U_000743"
     CDS             complement(15497..16897)
                     /locus_tag="ACGE4U_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFF8392929.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17058..17795
                     /locus_tag="ACGE4U_000744"
     CDS             17058..17795
                     /locus_tag="ACGE4U_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFF8392930.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17920..18399
                     /locus_tag="ACGE4U_000745"
     CDS             17920..18399
                     /locus_tag="ACGE4U_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFF8392931.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18396..19163
                     /locus_tag="ACGE4U_000746"
     CDS             18396..19163
                     /locus_tag="ACGE4U_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFF8392932.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19393..19962
                     /gene="lepB"
                     /locus_tag="ACGE4U_000747"
     CDS             19393..19962
                     /gene="lepB"
                     /locus_tag="ACGE4U_000747"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8392933.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20066..20668)
                     /locus_tag="ACGE4U_000748"
     CDS             complement(20066..20668)
                     /locus_tag="ACGE4U_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFF8392934.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20774..21004
                     /locus_tag="ACGE4U_000749"
     CDS             20774..21004
                     /locus_tag="ACGE4U_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392935.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            20995..21243
                     /locus_tag="ACGE4U_000750"
     CDS             20995..21243
                     /locus_tag="ACGE4U_000750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8392936.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21349..21933
                     /locus_tag="ACGE4U_000751"
     CDS             21349..21933
                     /locus_tag="ACGE4U_000751"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFF8392937.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21936..23009
                     /gene="prfA"
                     /locus_tag="ACGE4U_000752"
     CDS             21936..23009
                     /gene="prfA"
                     /locus_tag="ACGE4U_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFF8392938.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23002..23841
                     /gene="prmC"
                     /locus_tag="ACGE4U_000753"
     CDS             23002..23841
                     /gene="prmC"
                     /locus_tag="ACGE4U_000753"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFF8392939.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
BASE COUNT         8139 a         4209 c         4834 g         6687 t
ORIGIN      
        1 agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct cacatttttt
       61 ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa taaagcttga
      121 cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc tatttaaaaa
      181 gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag aacgttgttg
      241 gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa gaagctggaa
      301 gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa
      361 agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat atcctttgtc
      421 gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga cgtatgggga
      481 ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag aataagaacg
      541 accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa tctgaagaaa
      601 aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat gttactaact
      661 taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat aatgaagaag
      721 agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa gctagcggag
      781 gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa ttcatgatcg
      841 atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt gatttcttct
      901 tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa tggtggttcg
      961 tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa ctttttcttt
     1021 cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc ttattctaga
     1081 gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc agtttttttg
     1141 tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa gaatctgata
     1201 gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa aaagatggaa
     1261 gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt agaaacattt
     1321 acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga tctgttgttt
     1381 gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg tctattagaa
     1441 gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag
     1501 acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg gataccaaac
     1561 agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga accaggtgag
     1621 caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg gttgatgcta
     1681 gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt ggaaccatta
     1741 ggaatgatga acagcttatt ccttcctttt ccttccaaaa aaatcgttcc cacagaacaa
     1801 ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag agttttggct
     1861 gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac ttttgtccaa
     1921 atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga gacgattgat
     1981 tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg atgggactta
     2041 aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac aggtacgttt
     2101 ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct
     2161 gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa
     2221 gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga ggagtttgaa
     2281 ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc
     2341 gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa gattgggcga
     2401 tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat
     2461 tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa ccagtgtttc
     2521 cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta catccagatg
     2581 atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg
     2641 atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga gaagaactag
     2701 ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca gttaaaaaag
     2761 gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga atcatgattt
     2821 tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac agaaaagatg
     2881 cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca actgtccctg
     2941 cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg atcgtcactt
     3001 atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc acatctttca
     3061 aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac cttgtcgttg
     3121 acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat ggcgtttctg
     3181 agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa tagttcccag
     3241 atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa atccgtccat
     3301 caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc
     3361 gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg atcatgccaa
     3421 atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt gtgtctggcg
     3481 atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt ttacccatct
     3541 tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa
     3601 ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga gcgatgatta
     3661 tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat gtgaaattca
     3721 aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt agcgttattt
     3781 gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg ttgctcgcct
     3841 ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg atcttagaac
     3901 ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca cctttaggaa
     3961 tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca aatcctggac
     4021 ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa aaagtaaatg
     4081 cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat tttccatttg
     4141 tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc ggcacgttga
     4201 tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca ctactcgtga
     4261 tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata gcaatcagta
     4321 catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa gaaaaagaga
     4381 acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat tccgtcattt
     4441 ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat
     4501 tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa ttgaataacc
     4561 aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac
     4621 ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg ctgtcacgaa
     4681 taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca gatatcacag
     4741 aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga cgcggctcac
     4801 aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta
     4861 tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa
     4921 gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt ctagttgaac
     4981 attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa gatgtattta
     5041 tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc tgtatctatc
     5101 caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga attagataat
     5161 cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag aggctcattt
     5221 gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga tgggaaaagt
     5281 aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt actgacgaca
     5341 gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac cactttttat
     5401 gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat cattcggaac
     5461 cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc gaatgtatta
     5521 gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc atcactgaat
     5581 tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt aatcggggaa
     5641 gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt ggttttgatg
     5701 ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga gacatataat
     5761 agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg gatcgcttct
     5821 gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt attaaatgac
     5881 ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca gcttgcttat
     5941 ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt ttatgaagat
     6001 gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag agccgtttta
     6061 caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata cgaggaagtt
     6121 gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt atccgaagaa
     6181 gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc
     6241 aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta
     6301 cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta
     6361 aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga
     6421 tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta
     6481 aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat
     6541 aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga tcaaatcaat
     6601 caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg
     6661 attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat
     6721 cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg
     6781 ttattccata catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa
     6841 catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt
     6901 atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga
     6961 gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt
     7021 tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat
     7081 acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt
     7141 gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc
     7201 aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca
     7261 tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga
     7321 tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc
     7381 tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat
     7441 cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa tgatttttga
     7501 acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac
     7561 ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat
     7621 gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac
     7681 aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata
     7741 gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc
     7801 atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt
     7861 tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa
     7921 gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga
     7981 gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac
     8041 aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg tatctcctga
     8101 ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt
     8161 atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta
     8221 aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag agagattacc
     8281 tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc
     8341 aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agagagatgc
     8401 gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac
     8461 tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga
     8521 tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg
     8581 ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat
     8641 tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta
     8701 tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca
     8761 aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg
     8821 cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa
     8881 tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag
     8941 aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt
     9001 tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca
     9061 acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat
     9121 gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca
     9181 atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca
     9241 aataatcaac agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag
     9301 accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag
     9361 ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg
     9421 caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt
     9481 ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg cctccaatat
     9541 aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca aaagttattg
     9601 tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac
     9661 cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc gtgccaaagt
     9721 acgactattt ctctttttag taagcggata caattagagt atcaaaagga gagagggaga
     9781 gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa
     9841 ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt
     9901 gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt cgctaccatg
     9961 gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta
    10021 attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga
    10081 acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc
    10141 aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat
    10201 aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt
    10261 ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc
    10321 catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat
    10381 gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa
    10441 tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt
    10501 actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt
    10561 ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta
    10621 tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta
    10681 agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct
    10741 taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca
    10801 gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag
    10861 acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc
    10921 tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc
    10981 gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa
    11041 gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa
    11101 gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat
    11161 tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct
    11221 gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag
    11281 attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa
    11341 gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta
    11401 aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg
    11461 gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa
    11521 tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc
    11581 cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt
    11641 gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat gccatggtag
    11701 atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag
    11761 atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg
    11821 tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt attgcaggag
    11881 tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt gatatggaca
    11941 atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat ctagcaattg
    12001 cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc ggtcgaggat
    12061 ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat gtgaatcaca
    12121 cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca gatgaatcaa
    12181 aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag
    12241 taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg aatatcttac
    12301 cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa
    12361 catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag
    12421 aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc tttccaaatg
    12481 cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc gtccaagtgg
    12541 aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa atccaattaa
    12601 aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta tttagtgtca
    12661 atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag accattgatg
    12721 aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag gaaacaggaa
    12781 gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat attgaaaaaa
    12841 tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc gctcggacac
    12901 cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa ctacaagaac
    12961 ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat tactatgatc
    13021 ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac ttggaacaat
    13081 tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg
    13141 tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa
    13201 atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa gatagggcag
    13261 atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag ctgccaggtt
    13321 ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc ggtaaatgac
    13381 accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc ccaaaggtcg
    13441 ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat aagtttcatt
    13501 gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct gacttaacgt
    13561 gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca tgtagtagtg
    13621 acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt cgatatagtt
    13681 gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct caaagtcttg
    13741 accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat gtatactaac
    13801 gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca tcaaaaggtt
    13861 tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat tgagttcata
    13921 attagccacg gttcattcct cctttgataa atccttgagc agctagttct ttacgaagct
    13981 gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat tctttgattt
    14041 tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga atcccggcaa
    14101 cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca gctgggatat
    14161 ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg attccgtctg
    14221 cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga ccaatcatat
    14281 cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat tttttcccat
    14341 tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa ccttgtttga
    14401 ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa gcattgtaga
    14461 cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg aaatctgctg
    14521 acacattgtc catccgtacc atatcagtta atcgataatc caattcagga cgtttgaagt
    14581 cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta taatgcaaga
    14641 tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt tgttcacgat
    14701 cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata gaattgaaga
    14761 ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca ccgtctacaa
    14821 tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg aaaatatttg
    14881 taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt gcttcatcta
    14941 tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag aaagtcgata
    15001 caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg aattgctggc
    15061 gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg gtgacaacga
    15121 cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc agcgctagtt
    15181 tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc gacgtttttc
    15241 caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa acgatttgct
    15301 gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt gcttttctta
    15361 ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac
    15421 aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat tattccctct
    15481 tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca tagcctagat
    15541 ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc cgcccaataa
    15601 acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta atccctaaag
    15661 ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc agcgtttgtt
    15721 cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa agcataaaga
    15781 caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata ttgatgttat
    15841 cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta gttgttcgaa
    15901 tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa aaaatgatca
    15961 gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta cgagtcgtaa
    16021 tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg aataaatggt
    16081 ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca aaagctaaca
    16141 cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa cgtttgtata
    16201 atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa
    16261 acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa tccccagtga
    16321 cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa aaaacggcat
    16381 cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa
    16441 gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg ataccagaca
    16501 aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt
    16561 tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca acttgaaata
    16621 atatttccag cagtataata ccgttatcat tgaacacaga attgatatca aaagtcgata
    16681 gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa gaaacatggc
    16741 tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc gtcatcaata
    16801 tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc
    16861 gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc tgacaatagt
    16921 cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca aacagataac
    16981 aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta gaaaaaagaa
    17041 ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa
    17101 aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa
    17161 agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca ttggaattca
    17221 tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat cctaaaagtg
    17281 agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat gagaaaactg
    17341 accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc aaaatggttt
    17401 taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata
    17461 tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac tttggaagtg
    17521 ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga atggatatcc
    17581 aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac
    17641 gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt ggttgcgtgt
    17701 tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca aaagcttact
    17761 taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga
    17821 aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc gtagtaaagt
    17881 aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt agcacctatt
    17941 ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac aggtattgtg
    18001 aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga aacaacacca
    18061 actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga aaccactacg
    18121 acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata tacaactgac
    18181 agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc aaccaatcca
    18241 agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac gactgtagat
    18301 gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat
    18361 acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta gaccttatca
    18421 acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt aaccgtggat
    18481 atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat ggactttggc
    18541 aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg cttgtttatt
    18601 ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc gatgtcactc
    18661 agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa gaatcgatag
    18721 ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct tatgacgaag
    18781 attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt gagatcaaaa
    18841 caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg tatccttatt
    18901 actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga agctactcgg
    18961 acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg gaacctgtcg
    19021 gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat gagccttatc
    19081 gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt tatagtcgta
    19141 gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa
    19201 acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt ttttatataa
    19261 cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa cattgtagaa
    19321 taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga aaatacaagt
    19381 aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct tgtattagcg
    19441 gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg
    19501 gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa ctatgaacga
    19561 atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt taaacggatc
    19621 atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat taatggcaaa
    19681 aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga taagttacaa
    19741 ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc cacctttacg
    19801 agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga taaccgcttg
    19861 atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca aggaaaagtt
    19921 gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag
    19981 ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat ttcttggagt
    20041 tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag aaaagcgaag
    20101 cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta gcagagacaa
    20161 agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta tggactttcg
    20221 gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg ccaggagcta
    20281 aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa agtacaagtg
    20341 atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca gcgtggatcg
    20401 ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc agcatagttt
    20461 ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa ctaacaagcc
    20521 cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt agataagtag
    20581 ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca taatggttag
    20641 cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac tttgaaattt
    20701 taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca aaaagggaga
    20761 atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag gaaagtgtgt
    20821 ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa cggaaaaata
    20881 tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag aatatttagg
    20941 ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta ttgtatggga
    21001 ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga agcagaaggt
    21061 tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg gcactactct
    21121 atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt ggaagttttt
    21181 ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt ggaacaaaag
    21241 tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta aaaaatgctg
    21301 aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat ggcacaacta
    21361 ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa agtcgcacat
    21421 aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga tacaagagat
    21481 ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc gatatttgaa
    21541 gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg tgtgctgatc
    21601 gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat tgtggacgaa
    21661 ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga gttatttgaa
    21721 ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac aatctgttgg
    21781 ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc tgtttatgaa
    21841 ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg aaaacattac
    21901 tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta caagcaattg
    21961 aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt tctgatacaa
    22021 agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc gaagtttatc
    22081 gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt ggtgaaaaac
    22141 tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa aaagaaaaag
    22201 aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat gacgataaaa
    22261 atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta ttcgctggcg
    22321 atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca gaagtgatgg
    22381 aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc accggagaca
    22441 acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt gttccttcaa
    22501 ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg ccagaagctg
    22561 aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat catgcttcag
    22621 gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact cacttaccaa
    22681 cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt gaaaaagcaa
    22741 tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa agcgagtatg
    22801 atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc cggacgtata
    22861 atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa aaattagatc
    22921 agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat gatcaaacat
    22981 caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct gtcacgggct
    23041 tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt atttttggaa
    23101 cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat ttcagaagaa
    23161 gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc acctcagtat
    23221 ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc aacactgatc
    23281 cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc tggtgtaccg
    23341 ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt gaaagcagca
    23401 agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga agtagcgaaa
    23461 gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac attaggacca
    23521 gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag tcggaatgaa
    23581 tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct ttttgctgag
    23641 aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt actaaaatca
    23701 aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa aaaaatattt
    23761 cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg caatgaacga
    23821 atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgt
//
LOCUS       DAXWLF010000025        23240 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXWLF010000025 DAXWLF010000000
VERSION     DAXWLF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.025"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..226
                     /locus_tag="ACGE4U_000754"
     CDS             <1..226
                     /locus_tag="ACGE4U_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392940.1"
                     /translation="VAEVQKLGKSMWTEKPKRRTPRKIAIWHDDRILVTGTAEQLSEI
                     TGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            226..582
                     /locus_tag="ACGE4U_000755"
     CDS             226..582
                     /locus_tag="ACGE4U_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFF8392941.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            542..787
                     /locus_tag="ACGE4U_000756"
     CDS             542..787
                     /locus_tag="ACGE4U_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392942.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            784..1203
                     /locus_tag="ACGE4U_000757"
     CDS             784..1203
                     /locus_tag="ACGE4U_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFF8392943.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            1200..1757
                     /locus_tag="ACGE4U_000758"
     CDS             1200..1757
                     /locus_tag="ACGE4U_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFF8392944.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            1754..2050
                     /locus_tag="ACGE4U_000759"
     CDS             1754..2050
                     /locus_tag="ACGE4U_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392945.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            2127..2540
                     /locus_tag="ACGE4U_000760"
     CDS             2127..2540
                     /locus_tag="ACGE4U_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFF8392946.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            2849..3001
                     /locus_tag="ACGE4U_000761"
     CDS             2849..3001
                     /locus_tag="ACGE4U_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392947.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(2998..3273)
                     /locus_tag="ACGE4U_000762"
     CDS             complement(2998..3273)
                     /locus_tag="ACGE4U_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392948.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            3727..3933
                     /locus_tag="ACGE4U_000763"
     CDS             3727..3933
                     /locus_tag="ACGE4U_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392949.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            4129..4296
                     /locus_tag="ACGE4U_000764"
     CDS             4129..4296
                     /locus_tag="ACGE4U_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFF8392950.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            4322..4666
                     /locus_tag="ACGE4U_000765"
     CDS             4322..4666
                     /locus_tag="ACGE4U_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFF8392951.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            4671..4952
                     /locus_tag="ACGE4U_000766"
     CDS             4671..4952
                     /locus_tag="ACGE4U_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392952.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            5055..5369
                     /locus_tag="ACGE4U_000767"
     CDS             5055..5369
                     /locus_tag="ACGE4U_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFF8392953.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            5347..7041
                     /locus_tag="ACGE4U_000768"
     CDS             5347..7041
                     /locus_tag="ACGE4U_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFF8392954.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            7061..8239
                     /locus_tag="ACGE4U_000769"
     CDS             7061..8239
                     /locus_tag="ACGE4U_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8392955.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            8202..8888
                     /locus_tag="ACGE4U_000770"
     CDS             8202..8888
                     /locus_tag="ACGE4U_000770"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFF8392956.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            8888..10048
                     /locus_tag="ACGE4U_000771"
     CDS             8888..10048
                     /locus_tag="ACGE4U_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFF8392957.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            10058..10933
                     /locus_tag="ACGE4U_000772"
     CDS             10058..10933
                     /locus_tag="ACGE4U_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392958.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFLEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYRKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            10930..11241
                     /locus_tag="ACGE4U_000773"
     CDS             10930..11241
                     /locus_tag="ACGE4U_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392959.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            11231..11584
                     /locus_tag="ACGE4U_000774"
     CDS             11231..11584
                     /locus_tag="ACGE4U_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFF8392960.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            11574..11975
                     /locus_tag="ACGE4U_000775"
     CDS             11574..11975
                     /locus_tag="ACGE4U_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392961.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            11968..12372
                     /locus_tag="ACGE4U_000776"
     CDS             11968..12372
                     /locus_tag="ACGE4U_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392962.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            12384..12992
                     /locus_tag="ACGE4U_000777"
     CDS             12384..12992
                     /locus_tag="ACGE4U_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8392963.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPNNMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            13012..13374
                     /locus_tag="ACGE4U_000778"
     CDS             13012..13374
                     /locus_tag="ACGE4U_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392964.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            13377..13559
                     /locus_tag="ACGE4U_000779"
     CDS             13377..13559
                     /locus_tag="ACGE4U_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392965.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            13577..16897
                     /locus_tag="ACGE4U_000780"
     CDS             13577..16897
                     /locus_tag="ACGE4U_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8392966.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SSVFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAG
                     PEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            16948..17685
                     /locus_tag="ACGE4U_000781"
     CDS             16948..17685
                     /locus_tag="ACGE4U_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8392967.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            17695..19986
                     /locus_tag="ACGE4U_000782"
     CDS             17695..19986
                     /locus_tag="ACGE4U_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFF8392968.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            20010..22232
                     /locus_tag="ACGE4U_000783"
     CDS             20010..22232
                     /locus_tag="ACGE4U_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8392969.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVIFSLDGTFMSEAGYYKHCYVEYRKGNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGA
                     DYSPVDGKLILIRYRYVASGTGKIVLDLPINSNSITQEWKEFSITGTTPTFSPQAYHP
                     WIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDAPHYLSKTPLGENIANKSVTF
                     PINTSAYKLYEYNTEEDLIIGQTYTITLKGTKPASQTFAVYNYWNINLGSLKPVEGLT
                     DVWSITFTPTTLEPGLPKDLRILQSPKETAGACQIDWLKIEKGDTRTPNIEQYKYRGI
                     GMRDSNNPKDYVWDLAPEYVENNLATDIKVAEITGKANNYTDGKVSQINSQLTASINE
                     VDTTAKGAQTKANANATAIDELDNKIDERINDTATTAFYGAQGEVSNKTKVAEYGVGT
                     GYTTTAVGETHFERQSNGYVKCLKAGKYSISAQIRVQLGRKYASWLYTDLYKDGTSVD
                     NLVSYGVDALQNRFAASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTLVLTRLSD
                     "
     gene            22310..22552
                     /locus_tag="ACGE4U_000784"
     CDS             22310..22552
                     /locus_tag="ACGE4U_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFF8392970.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            22568..22765
                     /locus_tag="ACGE4U_000785"
     CDS             22568..22765
                     /locus_tag="ACGE4U_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8392971.1"
                     /translation="MVLPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            22776..>23240
                     /locus_tag="ACGE4U_000786"
     CDS             22776..>23240
                     /locus_tag="ACGE4U_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8392972.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPG"
BASE COUNT         8207 a         3483 c         4952 g         6598 t
ORIGIN      
        1 agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac
       61 gccgagaaaa attgcaatct ggcatgatga ccgaatttta gtaacaggta ctgctgaaca
      121 gttatctgaa attactggat taagcaaaaa cattatctgg gatagagcta ggagcttatg
      181 gattgattca aaaggacgac agtttaggta tgtggaggag agataatgga tctcattaca
      241 caatacagtg acatcatcct caagaaaatc atgatgaaga ttcagaaaga taaaaaatca
      301 aaagaacgag ctgaattagt taagttagaa atggctgaaa caggagcagg agtgcgaagt
      361 agcaggcatt ggaaagcagc agcaaacatt gaattttatt acaacgaaat tcaaaaaggg
      421 ttcgatcaga tgcgtgagct ggatcggcaa acaaattgga gcaagaaact tcatcaagat
      481 cgtttcaaat ttgtagagaa gtataaagaa atattagaag agtatttgag gaggacagca
      541 aatgataaaa aaactcgttc aattcagcat ggatttatat gatatcgaat caggagcaac
      601 actatctgtg gaatcggacc atctaatcat aaattttggt ggaaagcgcc agattatttt
      661 gtgggtagtt gatgatgtac tgtttccaga aattgttcat gatttcgaag aatcaaaagc
      721 ggttgagttt gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg aggaggacag
      781 cgaatgatac cgaagtttag agcgtgggat aaacgaaaga acgtaatgag agatgtagcc
      841 gtcttgcatt ttactaaaaa cggcaaaaca aactttattg aatattggat aaatcctacc
      901 gaattgaaat catatcacgt gcgaaacatc gacctcatgc aatccacagg aatgaaagat
      961 aagaatggtg tggatatatt tgaaggggat atagtattag tcagcgtgcg aaatggcttc
     1021 gattacttag ataataaagt ctgtattgtc aaaaattcaa tagattattc cggattagtt
     1081 tgtgccactg ttgatgaaga cttagagtat caaattttta acacagagct gtttgaagaa
     1141 tacacgtacg aagtcaccgg aaatatatac gagaatagcg agttattgga ggaacagcga
     1201 tgaataaaca ggaagcaatc aaaaagttag aaagtattaa agcgatagga aatgatgcaa
     1261 tagctgcttg ctataacgag agtataaatt caggtattac gttaatgaaa aaaatagacg
     1321 aaccgcagaa accagttatt ccacaactcg tggccggttg gcttgagaaa tctacggacc
     1381 cttttacaaa agctgaaaaa atagcgtatt taatcaaatc taaagatggt gattcatatt
     1441 atttctgtga ttggtttgta cgagatggca tagtgacgca agagcaagga gaagaattac
     1501 tcgcttgggc aacaagacaa tcatatgaaa cactattgag cctatacaac ggctacgagg
     1561 ttgagaaaga gccgttatgg gcaataaaga atgccgatgg aaactatctt actaaatgtg
     1621 ctttatgggg aaaagatgga gtaaattata gttttgaatg caatccatct catcgattgc
     1681 ttttcactga taaagcaaca gcggatgctg cagcattgtt ggtgaatgga acagtggaag
     1741 aggtggtaga aagatgaaac taaaagacgg attttacgct agcagtcacg gtatcggcgg
     1801 tttaatgcta gatatgccga caaagaaccc taaaacacgc aagaaatcaa aattcaaagt
     1861 cggtgacatg gtccgctgtg aagcagaaga gttcgtttat ccgttcagag gatacgttaa
     1921 aaagatactg tcaaactcag caatcattcg tattgaaaat acaatgaagt gtgatcgttc
     1981 gactgcaaaa cacaagcatt atttagctgt agcgagatta gttgacatgg aagtaatcaa
     2041 gagcaaataa aaaaagccgg atcgctccga ctgattcaat aaatccaaca catttattat
     2101 atcacataaa ggagcggttt gacttgatgc aattgttacg agaggtagat ttcaaacaga
     2161 caagatgtaa tgcgagagat gtgctgaaga actttcggcg tttggagcgg atggcaggtc
     2221 gctctttgat agatattaag tcgccgatta taacggatat gccgaaggca ccgaagcatg
     2281 gcaataaggc agaagacgcg attattcaga tgatggatat agaaacggag agagatgcga
     2341 ttctagcggc tttgatggct cttagtctga ttagtcgtca gatactctac tacagcttct
     2401 gtgtgccaga tagcttctca aactacagaa ttagccgtga agtgggttat tcagaaagaa
     2461 gtatacaacg gatgaagtcg gaagctctaa tagagtttgc agaagcatat aaacacggaa
     2521 aaataattgc ttataaataa tttggcggtt ttttggcgga atgatggcgg tttttagaca
     2581 tttaccagtg ttattatggt agtgtcgaaa gattaggaaa caggatcgac aaaataaacg
     2641 aagggaggca atctccctca tcgttttaaa cttcttctta gatagacagc aactaattat
     2701 gaagaaaagg atgtgaattc aactcctcct aaattcttca tcggttgctg tttattttga
     2761 ttagacagca gcactcacaa aactaaacat gttcttgtcg tctttcaact gctgctgtct
     2821 attaatttat gtattggagg gaagaaaaat gaaattatca gtagaaggca ctccagaaga
     2881 aatacaagaa ttgctccaag ctattggtag tagcaaggag caaaaggtaa gtgtttctaa
     2941 ttcagatatt gacggtattt gcaattgttt aaacagagaa aaaatatcta atccttacta
     3001 agagaaaata tgttcgttaa tgaatttcca attttcctct gtagctctac cttgataatt
     3061 tttttgcagt tcacagatga ataaggaatc gttattatca agtactgtat ttaatttttc
     3121 acacatttga tttggattca aattagtttt aactaaccaa aaagattttt gtagctttat
     3181 atatgcccca aattctttta ttatttcaaa tactttatca tacttttgcc ctgggttatt
     3241 aagatcataa ctaatcatgt agggtttata catgttgtct tgcacatcct tgttttatat
     3301 ttcagcggac cactcgctga taaataaaat tatacgctta gtatttattt tcacaatatt
     3361 aatttatcgt aataactttt gagtaaatag aacaaaaaac ctgcactagt ttccgctagt
     3421 acaggcagtg actatatcgg ttgataatct agcatatatt aaaaataatt gcaagaaaag
     3481 aaatttatag tggtttatac caaattatca aagcaatgtt attttgttgc tgtctattgt
     3541 tttttaatta ttcacacgat aactaaaggt gggtgaagag aaatgattcc attaataatt
     3601 tcaatttttg cgctctgtct taatgtctat atgattggat ttaaaaatgg gcaaaataaa
     3661 aaatagtagc agccaagaat aattttatag tgtcactgtg gcggaaaggg tagacgctaa
     3721 gcatgtgtgc taggtcaatg cttcggcaac catgcaaggt tcgattcctt gccagcgaca
     3781 ttaaatgcct atgacggtta cgactaccga aaaaagatcg ttaagaagct atacggtgct
     3841 acgtacggca atgtagtaag tgtgctatct gtacaccacc aagcttcggt cactgtggcg
     3901 gaagtagaag acgcagcggt aaatggcgag tagcctcgtg agagcctggt aagttctcgt
     3961 gagtggtgca atcccactcc agcgacttta agcaaccgag gcatcggcgg tttaaaaata
     4021 taggggtgcg caatttcgta cgcgttttgt gcatcgtgca agttactatt acatattaga
     4081 tcactctttg agtggtcttt ttatttttgc acaaaggagg aaacaacaat gtatagacca
     4141 caatacttag aacagaagta tgaagtaatc actgttcata aaggtaatgg cgaaaaagta
     4201 tatgagtata gaagaccaat aaagagcgat acatataaac gaaaggaaaa caatgaagtt
     4261 attccattgt atggcaaaag aatagctaag cattaaataa gattgcgaaa ggagacggaa
     4321 catgaccgag gaattctata gatggctatt acagttgaca agagaagatc gtttggttaa
     4381 gttctatcag tctcctaaat ggcgcaggct tagagagaaa gcgatgaaac gagatcacta
     4441 tgaatgccaa gagtgtagaa gactaggtaa gtatcataga gtagagaacg ttcatcatat
     4501 aaaggaagtc aaggatagac ctgacttagc tttagattta gataatctta tttgtttatg
     4561 tgttgaacat cataatgaag ttcatggcag atatcttaca gcattagata aacaagagaa
     4621 gaagatagaa agctttgcta acttcgatgc aagtgaaagg tggtaagtgc atgatcatca
     4681 atgataatgg cagagagtat gatacagaaa agattgaaga gtattcatct tatactcagg
     4741 gattaattaa acgtttgata tacgttcgct atgtaggtat tagggatctg ttatcagata
     4801 actgttgtag taaatacaaa gtgaatcaag taagagaagc gttgaataaa gataataacg
     4861 tcgaaagaat aaaaaatgtt tttggatata gtattgaaga gattaattat tacattgact
     4921 tcgctgaagc tttcattccg atggtgagat aaccccccct taaaataaat cgcaaatttt
     4981 ttgggggtga tgaaacggag ggggctgtca ggaaaagaga ttttttcgaa ctttatcatg
     5041 aaaggagggc taaaatgttt aaaaacgaat tgtctcaaaa tcgctacaga gaaaaattac
     5101 gccgctcttt aataagccaa ttggaaagtc agaaaacaaa tattgagcca ttcttagata
     5161 atgttgatcg ttatatcagt ttatgggaaa cggcgatatc actggaagaa gatatatccg
     5221 agaacggcat tagactggag aatggtaaaa agaatgaatc agtagcgttg cttgtttctg
     5281 tcaacaaaca aatgggattg atgttggata aacttgccat tactcctgaa ttggtaggtg
     5341 aagcaaatga atcaattcct gagttataag catattgaaa attggttcaa agctatagaa
     5401 gaaggcacta tcaaggtatg caaagagcaa ttattgctaa aaaattatct agaagaaaga
     5461 gtctttacta gagaagatat ttacttcgat aagcagatgg tagaggattc aatcaatata
     5521 ccagcacaat actttccatt cgaattaatt ccgtgggaaa aatttctaca atgttttatt
     5581 tatggtgttc gatggaaaaa agataaaaca ctagtgttca atagatatct ttcattaatg
     5641 ggacgtggta atggtaaaac tggttttgct tcttggaaca acttctttct actaaccgct
     5701 aaacacggta ttaaaaatta tgatattgat atctatgcca ataatgaaag ccaagcaaag
     5761 actagttttg atgatgtatt taaagtaatt aaagatcatc ctgatttaga taaaaaagta
     5821 tttaaagcta cgaaggaagt tattcaaaat atcgctacaa acagcaaact tcgttataac
     5881 acggcaaatg ctagaacaaa agatgggaag cgaccaggtg caaaccgctt tgatgaaatt
     5941 cacgaaaatg aagattattc aatgataaat gtggctactt ctggtggtgg taaaattcga
     6001 gattatagag aattttatga tacaactaat ggtcatgttc gtggtggtcc gcttgatgac
     6061 attatagaag aatcaaaaat gattctttct ggagaacttg gaattgacaa ggatggagca
     6121 gaattttcta gtttgtttcc atttatttgt cgcttggata acgataatga agttgatgat
     6181 cccgacatgt gggaaaaagc ttgtccaact attaattaca atgcagattt aaaacggaaa
     6241 atgtttcaag aatactctca aatgcaacgt aatgctggtt taagacttac gttcatgacc
     6301 aaacgaatga acagacctat ggaagataca cgatttgctg ttgcttcata tgatgatgtt
     6361 ctgcatacga aagaaaaaga atttcctgaa aaaatggatg aagtgatagg aacagtcgat
     6421 tttgctgata gacgagattt tgccagcgtt gggttgctag gaaaatacga taaagatgtt
     6481 tattttacac aacatacttt tatccacgaa tcagcccttc gattacaaaa catcaaacga
     6541 gaggttatag atatttctat agatcaagga aaatcacaga tcgttcatgg aaaaaatata
     6601 gaagctgatt atattgtagg ttggtttctt gaaatgagta ataaatatta tattaaaaaa
     6661 atcgctatgg atatgtaccg tgcaaaaata ttgaagcccg ctttagaaga agcaggtttt
     6721 actgtggaaa ttgttcgaag cggatctgtt acacatggta tgttaaaaga tctggttgat
     6781 gaccttttta ttaatcaacg tttatttttt ggtgacgatg cgattatgcg ttggtattgc
     6841 atgaatgtat atgaagagca tatttctaat ggaaatatac gctatgaaaa aatagaacct
     6901 gaaactagaa aaacggatgg ctttttttca ttccttcatg gtttgaattt tttagatgat
     6961 atttatgatt ctgctcctgt aacagtcaca aatagctcag tagaaaatac aggaactgga
     7021 tttactcctc tagtattcta acttgaaagg aggtgagaaa gtggggattt ttcaaaaggc
     7081 ggtaggatac ttcacaaaaa aagcaacggt tcctttagaa gaatactttt gtaaattgca
     7141 agttgatttt gtgtatcgaa aatttgcgat tgaaacttgt attgatttga ttgcaaatgc
     7201 gatgagtaaa gcggaattca agtcatatga agatggaaaa aataaaaaga atgatcttta
     7261 ctataggctg aatgtagctc ctaataagaa aaataatgca acagaattta gaaaaaaact
     7321 gatcaggaga ttaatattct acaatgaagt attgatcgtt tctccgtcta ataattctag
     7381 cgaaatattt attgcggata gttgggatgt cacagaatat gcattgaaag atgatgtgtt
     7441 ttctcaagtg caaattaaca acatagtcct tgatagagaa tttctagaaa gtgatgttat
     7501 ctatataaaa tacgcagatc aacaaattag gcaactagtc gatgcgtatt atcaagcgta
     7561 tgggaaactc atttctagtg ccatgaatgt ttacaagcgc tctaacgctc gtagatacgt
     7621 actgaaaggg aatttattcc gaccgcaaga caatacaaca caagatcaaa tcaataaaat
     7681 gatgacatca caatttaagg cttttatgga agctgataat gcaggtgcgg tatttcaatt
     7741 acaaaatgag tacacattag aagatttcag cggaaacttc caaagcaatt caagagatat
     7801 aaaaaactta atagacgaca tctttgagat gacagcagca gcgtttcacg ttccgaaaaa
     7861 cctactaaag ggagacatga gtgggttatc ggatcaagtg gacgcttttt taatgttcga
     7921 aatcataccg attgctgaac ttattcagga tgcgtttaac gctagtctct atgaagtaga
     7981 agaatacttg tcagggaatt ttgtacgtgt ggatacaact atgatcaaga ttactagctt
     8041 caaagatttg gttgacgcta ttgatgtagg cattagaaat ggagtattta caatcaacga
     8101 aggaagagaa cgcgttggaa atgatcgctc tgataaggcg atggcagatg aaatatttat
     8161 aactaaaaac aatcaacaag tatcgaaagg aggtgaggcg aatgacgaca atgaaaacat
     8221 ttctagcagt aaagaatgaa ggcgcagtac cgcaaatttt tattcaggga tttattggtt
     8281 ctagttggtt ctttgaaggg aatactgaca agggaatcaa aaatattttg gatagtctag
     8341 gtgatcaaga agaaattgaa gtagtaatta attcaaacgg tggagacgta tttcaaggga
     8401 ttgctattgg gaacttactt aagtcaaata aagcaaaagt taacgttgtg attaacggct
     8461 tagccgctag tgctgcttca attatcgcaa tggctggcga tactataaaa atctacaaca
     8521 atgcacaatt gatgattcac cgcgcttcca catacggaga aggaaatgtc gatgacttcc
     8581 gtacgattgc tgaccaactg gaatcaattg ataaatcggt aaaggcttca tataaaacac
     8641 gattcaatgg cacagatgaa gcattgcaag aacttcttga aaaagaatcg tttatggatg
     8701 cagaaacagc tttgagttat ggattggtcg atgaaattat cgatgcagaa aatagctcag
     8761 gtactgaagc taaaaaagaa caaagcgttg aagaaatttt gaatgacgtt aaagaaaaaa
     8821 gagcagaaaa aattgctgca tttacagcag cattaaataa aacatttgga caaggagatg
     8881 taaaataatg acagttaaaa atttaaaagg tgtaacagct gcaagcgacc aattgatgaa
     8941 agcttttaaa gatggtaacg aagaatcttt tagcgcagct atggtaagct tatctaagga
     9001 aattcaggat aaaattttag aagaagcaac agcaaaaaat caagatcaat tagtattaat
     9061 gaaccgtggt cagcgtgtat taactacaca agaaacaaaa ttctataacg aagtagtgaa
     9121 aaacgaaggt tttgcagggg tcgaagaatt agtgccagct actgtatttg aacgtgtatt
     9181 tgaagattta gaacaatctc atccactatt gcaaaaaatt acttttgtta acacaactgg
     9241 tgtaacagaa tggattgtgt cacgtggagt caatccagca tggtggggta aactttgcga
     9301 agctgttaaa aaagttttag ataatggctt tgatgtaatt aacatgaagc agttcaagct
     9361 atcaggttat attcctgtat gtaaggcaat gcttgattta ggtccagtat ggttagatcg
     9421 ttatgtccgt actgttttag tagaatcatt gagaattgca ttagaacaag caattgttga
     9481 tggtactggt aaagatatgc cagtcggaat gatgcgtgac atgagcaaac aaactagcgg
     9541 agaatatgct gaaaaaaaag cagaacctat tacagcttta gatgctgtaa ctatgggcgg
     9601 tttgatggcg cgactatcaa aattcaatat cgaaggtgtg aatgatccga tttatcgtaa
     9661 tgtgaatcct tctgatgtgg tcctaattgt gaatccaact gattactggt ctaaagtatt
     9721 cccagctaag actgtactaa ctgctaatgg agaatacgta caagtattgc cagtaccagt
     9781 ttcagatttg cagtcaacgg ctgtgccaga aggaaaagca gttattgggg tagcctcaga
     9841 ttacttcatg ggtgtaggat ctacactaaa aattgaagct tcagatgaat accattttgt
     9901 tgaagacgaa cgcatttatc tagctaaaca atatgcaaac ggtcaaccta aacgtaacga
     9961 tagtttcatt gtattagata ttagcgcttt gggaactact actacaacta caaaaccaac
    10021 aaccacaaca actacaacac aagcgtaggt gatcagaatg aagtatattc tttgtcagcc
    10081 ggcaatcaat cggtttaaat gggagcttga agtttgttta actaatctga agaaactagg
    10141 aatcaaagat atcgtattgc ttttcagcag acacgatgat cagattccta tttttcttga
    10201 gaaggaatat ggtgttgaag ttcatgtgta cgatgatctg cgggacgaca aagagtatat
    10261 tccttcgatt aaaccatatt tatggtggaa atatttagaa gaagatcatt cgcgtgagga
    10321 cgaccgatat ttctatatcg attcggatgt cattttcaat aaaagaatta atttgcgcaa
    10381 attgccttct aaagatgatg tttggtattg tagcgactgc tgtagttatc taagtcttga
    10441 ttatattaga agctgtgaaa acggagaaaa tattctaaaa gatatggcaa acattgtaaa
    10501 tgttacagta gaatctttgg aaactataaa cactaattca ggaggcgcac agtgggttat
    10561 taaccgtcct aaagcgaatt atcggaaaaa ggtttatctg gattctaatc ggctatatcg
    10621 ctaccttaga gggcaaaaaa caaatataca aatctggaca gccgagatgt gggcacagct
    10681 ttggaacatg atgtatttca atattggtcc taaagttcac gaggaattag acttttgttt
    10741 tgctactgat ccaatagaaa aagttaaaga agtaaaaatc ttacacaatg ctggagtaac
    10801 aacaaacgat gaagatttat ttttcaaagg gagatacgtg acttctacgc cttttgatga
    10861 agatttatca tttgtaaaca agaaaaaatg ctcttacgca tatgttaaag caattaaggc
    10921 ggtggttaga tgacgcctga acaagtgact gaagaattgc taatagctgt gaaggataat
    10981 atttacgtta cctggaacga agaagatgag tcaattaaaa agatgatagc taaaaatgct
    11041 gtttatcttc aaagtaaagt gagtacaaca ctttcttttt ctcctgaaag cttagaatac
    11101 ggattgctaa tcgaaagatg tagatacgac tggaatcgtg ctttagatga gtttgaacaa
    11161 aatttcgcta gtgagttatt aggtttcatt caacattatg cgctacaaga atatattgca
    11221 ggtgatgtga atggcgaata atcgtagact cgaagaaaca ttcaacgatg gttggttaaa
    11281 gattttgacg caaactacca aaagaaatga actaggaaaa aagattggtg tagaagatac
    11341 agaaatcact tctttaaaat ttagaaatct ttccatgaga gatagtgata taacagctat
    11401 ggatgcgatg ggatcgaaat taactaagaa agtaaagact ccatttcatc caatcgccaa
    11461 gaaatttaat aaagatcaat attttatcgt aatcgatagt atgcgttaca acgttatcta
    11521 tgccgattac gataattttt atatctattt ttatcttgaa agtgtgggtg aatatggtga
    11581 ttgataattc taaagaaaaa gaacgtttaa ataagcaaat ttctgctatc aaaacttcct
    11641 tagaagagca ttttggcctc aaactctttc aagactccgt aggcgaggat gagctacctg
    11701 atgattttaa ttacttcatt ctcgaaacag gagaaataga aatgatcact gagccaaaat
    11761 atagcgtggg tcaaaatcta tatctaactt tctattcaga aaatagagaa gatttaacag
    11821 gagactcact agatattatt tcattgattc aaaatcgttc gattcgtttt cagagaatgg
    11881 atcccaatca tttaaaacta gagaaccaag atcgctatat cgatcaattg gtatttacgt
    11941 ttagacgatt attgaagagt gattgtcatg gctaaaaata gttgggagct aaaaataaat
    12001 ggacatgatg aacttcttgt gcggatggaa cgctattcaa gcgagagcga acgactgatt
    12061 aacgaagcat tgaaatcaaa aggttcggct attgcagtgg ataggattac agaaaaaatt
    12121 cctgtttctg aagcagattt aagaagaggg caccaacacg caaaaaatag tcgtccactt
    12181 aagactcaat acattaattt gggtttcatc attagaccta caagaaaatt tgagtattta
    12241 aaatatcctg atttggggat aggtacttct aaaagaaatc agccggacga atttatgaga
    12301 agaggattag gtcttgcact tgatccaatt acagaacttc tgattcgtca attcgataaa
    12361 ttaaataaat agggggaaca acaatggcta aaacaaaaac tgtagtaaca acgttcgata
    12421 acgtaagtat caaacgaatt gcttttaatt ttaagaacgc aggaaatgca atcgcaacga
    12481 attgtaatgg acaattagat ggcgaaacag aaatgcaaac ggtggttaaa aaatgtggag
    12541 cgacagaagt aaaatcaaaa tctaaaccaa acaatatgac ggtaacaatt actgcacatg
    12601 taccgatgga agtttatcga cgtttcaatg ggttgaaaca agatggacgt attaaaccag
    12661 gcatttactc ttacggtcct gattccgtag gcgaagattt ctcacttgct gcagagatcg
    12721 tggatgactt cgaagaaaat agaaagttag ttggtatgtt agcatgcact tcgaatacag
    12781 gattaacatt ctctattgaa aatggtgcgg atgaagtagc tgcgttagaa ctagaaacaa
    12841 aagttatgca agatgaattt ggtaaatttt atcatgaagc aattgttgca gaacttgaag
    12901 aagacttaac agatcaatgg atgacgaatc tatctgctga tgtgattaaa aaaacttcag
    12961 ttgtgacaac tacggccact caatcacagt aaaaaaaacg gaggtagcga aatgaacgaa
    13021 gattactcaa aaattgaact aaacgatgga acaattttga atttagaacc taaactgaat
    13081 atcaagaaat tattgatgat caatagagat tttaacacag acgagtttgc aaaaatgtcg
    13141 atgggaaaag gctctatgga tattacagtt attcaaggtg caaaagccgt atatgtagct
    13201 tatcgtcaag cgaacatggt cgattacatt tcattcgatg aatttataga taaatgggat
    13261 tttgatatgg aggttgcagt agctgtatac agtactatga tgttcaaaca agcacgtgat
    13321 gcctatcaaa aagaattcga aaaagcaaat aaggaaaaaa agcttcaaaa gtaaaaatgc
    13381 caaagctctt agttgaaacg tgggtcgatg tctattcgat gttgaccgac gttttttcta
    13441 tgccttcaga tttggtttta agcgatatct gtttagatga cattttgcaa atggcttaca
    13501 acaagagcgc ttatgaagga tggaagaact atgcaataaa tcaatcccag aaaaactaaa
    13561 ggaaaggagg taaaaaatgg ctaaaaagag aacggaagca gaagtaactt tcatagctaa
    13621 cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta aaaatagagc
    13681 agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag acaagttagg
    13741 aagtaaatta tcttctttag aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac
    13801 tagtcaacgt ttagccaatg ccaaaaaata ttatggagaa aattccaccg aagttcagaa
    13861 acttgaaaga gaactgatta atcaacaaac agctcagcaa aggttatcta atgatataga
    13921 taaaacgagt aaggcattgg ctcaatctaa aggtgaattt aaaacgtatg agtcaactat
    13981 gaaagactta gataatgagc aacaacatct aaagtcaagc gcatcattag tagaatccga
    14041 atataaaaaa tggcaagcaa ctgctggcca gtctgctaca gaatctgaaa aattagcaaa
    14101 agcacaagaa tatgtcggca aacagagtga tattgctgaa caaaaaatcg aagtgctaaa
    14161 aaaacaatta gatgccacac aaaaagaatt tggagaaaca tctaccgaag ctttagaaat
    14221 gaaaactaag cttaatgatg cggaaagaga attcgaagaa ttaagtaatg ctgctaaaaa
    14281 cgttgatacc tccacagtag atgatatcgg caaaaaactt gatatgggta atttaatgga
    14341 agctactgat cacttgtcag tgatcggaga taagcttatt gatgtaggta gtaagtctat
    14401 tgaagcagct ggaaaagcac aagctatgca ggcccaattt aaacaagtct ttggctcttt
    14461 agaaggggaa gcacaggacg ccgttgaggg aatggctgaa gaatttggaa tgttaccaaa
    14521 tacgatcaag cctgttttta cacaatatac gtcaatgttt aaaggacttg gatatgatac
    14581 caaagaagct atggagttag ctggtgatag tactcagtta gcagcagatg cagcagcttt
    14641 ttacgataag tctatggatg atgctagtga atctcttaat tcatttataa aagggaacta
    14701 cgaaggtggg gagcaaatag gtttatttgc taatgatact caaatggcag cttatgctgt
    14761 taagcataat ttgataccag cgactgaagg agcaaaaaaa gccagtgaag agtcattgtt
    14821 agctgttgaa aaagcacaat ctaagtatgc tgatgctatt aagaaacatg gtgaaggatc
    14881 tttagaagca agagaggctg ctttaaaact taaagatgcg caagataaaa taaatgaaga
    14941 attaggccca caaacgcaaa aatggtctga tttagatgag gctaccaagc aagcagttcg
    15001 agttcaatat gctgaagata tgcaaaaatt agccggtgct acaggtcagg ctagtaggga
    15061 gtctgatggt ttagaaaacc aaatgacaag agcaaagcaa gcgttagaag atttttatgc
    15121 ctcattgggt gaagatatac tgcctgtgtt tatcaaaggg ttgcaagcag gagcgaaagc
    15181 tttgcaagga ttagccgaat ggtggagtaa acttgatggg ccaatgaaaa atttcatttt
    15241 agctcttgga ggaattctag cattattaag cacattagct cctgttataa ccgcagttgt
    15301 tacgatagtt ggcacatttg gttctacagt tttgctgcca ataataggaa ttatagcggg
    15361 cgttgcagct gtaatagcga ttgtaattac agcgtttcaa aactggggcg caatcactga
    15421 ctggtttagt gatttatgga aaaaatttac cgattggtta ggtgatacct gggaaagtat
    15481 aaaagaaagt gcctcatcag tttgggatgg agtcaaagaa acctggtctg gatttgtaga
    15541 ttgggttcaa gaaatttggc aaggagtttc tgattggttt ggagagttat ggagcggatt
    15601 agttgaagga gcttccaaca tctggcaagg agtccaagag acttggcaag cattcgttga
    15661 ttgggtttca aatatttgga acggagtcaa agaagtatgg tcgattattt gggcagacat
    15721 tgtaggaatt gttcaaatac catggacatt aataacgtca ttgattcaag ctggtattaa
    15781 tattatcgtg ggtatttttg atgtagctgg acagttatta ggcgcagctt ggcaagctgt
    15841 ttggacacct atttctgatt tccttaaaaa tacttgggat actatgacac aatggataag
    15901 catcgcttgg aatggaattg taactacatt ccatactata tttgatccag tagtggcatg
    15961 gtggaatggt atatggacat ctattagtac tacggcttca aatatttgga actcaattag
    16021 tgcaacagct tctagtattt ggaatagtat caagaataca atcactagct tggtacaagc
    16081 agctgctaca gtaattcaaa atatttggtc aactgtatct agttggttag gtggaatttg
    16141 gaattcaatc agctctacag catcaaatat ctggagtagc gtgactagca gtataagcaa
    16201 tgctataaac gcagctaaaa gtgccattca aagtgtttgg aatagtatat cttcgtggat
    16261 cagcggaatt tggaacggta tcaaaaacac tgctttgaat ctttggaatg gaattacaag
    16321 cactattagc tctaaagtaa acgatggaaa aaatgcaatt tcaagcggtt ggtccaatct
    16381 aacaggtatt gtttccgaca tattcaataa tgttaaaagc acgatagcta atatatggga
    16441 aggtatcaaa aagactgtta gcgctccgat tgattggatt agagataaaa tcagtagtgt
    16501 ctttgataat ttgaatattt cgataccaca tattccgtta ccacatttta aattgagcgg
    16561 agaattcaat ccattgaagg ggaaaatccc aacattgggt gttgattggt atgcgaaagg
    16621 tagtgtgttt aattctccaa atattatcgg tgtcggtgaa gcaggacctg aagcagtttt
    16681 acctttgaaa agatctgtgc tgcaagaaat tggggatcgt atcttgagta gcacatcagt
    16741 ttcatctagg gcacaaacga ttcaacctgt gaataactac gaattcaatt tcacaattga
    16801 tggtaacgca gatgaggtta ctatgaagca aacaactcaa caaatcattg atagcattac
    16861 aaaagttcaa aatgataatg cttcggcatg gcgttaaaca ggagagtatt tctcctgttt
    16921 ttttagtttt aaaaaggatg tgaaaaaatg actgattgta tacattctat aatcgatgga
    16981 tttcctgatt atttgcataa attggcttta gcggaaagac caaccatacc ttctccaaaa
    17041 agacagagag ttgaaacttc tgttttagga cggctaggtg gcttagtaca agattactcg
    17101 tttgaagata tgtcgtttac attgcactat aactatttag aggatgtgga agaccatcaa
    17161 gcgttcaagc aatcgtttta tatcatgcgt cattggttaa actatgcaaa gaaattagaa
    17221 ttctctgacg atcccaacgt ctattacgtt atccagacta tcgatattgg ggatgcagaa
    17281 aacgatattg ttgaatgggg agagttcgat gtaaatatta ctgcgaaacc attcgcaaga
    17341 gttcaagaag atgtacctat aaccgtagat aaaccacagt catttaactt gctgaataat
    17401 agtttagaag aaagttttcc aaagattatc atcactcctt cagctacttc atgccagttc
    17461 atcttaaatg attatgtgtt tagtttcgaa ggcttagtag caggaactga tgtagtcatt
    17521 gatagtgatt tgatgctttg ctacgaagag caatcggacg gagatatttt agatcggtcc
    17581 aacaaaatga agaccatgca atatccgaca ttgcaagtgg atatcaatca ttttaattgc
    17641 actggtttga gcaaaataca aatttatcgt aacgggttaa ggtaggtgaa atagatgatc
    17701 gataatttaa taactattta cgataaaaat gacgcgaata atttagctga acatttatat
    17761 gatacgcaag gtttaggcgc tttgtcagac tggttaacag ctactgttag caataaacta
    17821 aacggagccg agatatttca gggtacttat tcaataaacg gaactaatgc agatttgatt
    17881 gtagaaggac gtattattca gtgttatgta gatgaaaatc gagcaaaaca gcgtctacgg
    17941 atttattatg caaaaacttc tgtaataggc aatacgatag aagtaaaagc tgaacctatt
    18001 ttcaatgata taagaaaatc ggtgttgaat aaatatgaca gcggaacaga aaagatcact
    18061 gctactcagg catggcaaaa cgcaaaagtt ttagcgaaac cagctatccc ttcgcagttt
    18121 tctttctcgt cattagtaga tacgcttgct aatgtgaaga tagaaaaggc gaatttttta
    18181 gaattctttg gtggaaaaga gggatctatt ctagatcgat ttcatggtga gtttctaaaa
    18241 gataataaca cattacgtca tgaaaaaagt ctaggtacgg atcataaaat caaagcgatt
    18301 tatactaaaa acttaactgg tcttgactta gagattgatg ctcaaagtgt tttagttgga
    18361 gtttatccat tcattagcag ctcttcagaa ggagaagacg agatcactct accagaagaa
    18421 gttattttca cggattacgt ggatgattat cctgctggat atgtttcttt tgttgatttt
    18481 aaagacaaag cgactgatgt agccacatta agggaagctg ctaaagactg gttgaaaaca
    18541 aacatagata aacaaaaacc acaagtgagt ggttcgattg aattagtacc attgaggcat
    18601 caaagaggct atgaaaaatt tgttgatcta gaaaaagttt cgatgggtga cggagtagat
    18661 gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa ttgtggaata tacgtttaat
    18721 gttctaacca attcatacga taaattagtt gtaggaaacg tcaaaacaaa cttcttagaa
    18781 aacacagaga ataatgtcag caatttgatt aatgatgcca ttgatcaatt gaaaaacggt
    18841 ggcgaaatca gtgatttact caatgatatt gtagatcatc aaactgatat gattactggt
    18901 caaaatggtg gttatgtttt attagatcct aaagaagcgc ctagtcgtat tttgattatg
    18961 gacacaccag ataagaatac cgcaaggaat gttttacaaa tcaacaatgc tggtattggt
    19021 ttttctaaaa ctggcattaa tggaacatat gaaacggcat ggacgttaga tggcggattc
    19081 aatgcctcgt ttattacggc tggtgaaata gtaggaatta ctattagagg tactacatta
    19141 attagtgatg gtgctgatta tagaacaagt attgctaatg gcaaaatgac ttggtactca
    19201 aaaaaagtta acaaagatat tatggagcta gaagcacgtg attatgtaag tgctgatgcc
    19261 ggtattgtat catacaccat gaaaactggt ggtggtttca tgattagaaa tccacagggt
    19321 aacttggttt ttagtacgtg ggataatggt aataacagac cgtttttatc ttttggtgcg
    19381 ccaaatttca gatatagcaa tgctagttat gtaacttctg gcgatggtaa ttctttgggt
    19441 atagatggta gtgcgggtaa ctcatgggaa tttaaggtag ctggtaggac tatgaaattt
    19501 actagtgatg gtatgctaac gttaccaggt tgtttttttg gttcatggga agatgggaaa
    19561 cttgctaggt ttgaacaatc aacggtacaa gtatataaag attttactgt tagaggtact
    19621 aaaaactcaa ctgtaccaac agaacattat ggacaacgac tattgaacgc ttatgaaact
    19681 ccagaatatt atttcgctga ttatggggaa gccgttacag gtgacgatgg taaagttcgt
    19741 gttgatattg accccatgtt tgctgagaca gtaaatctaa gtcggtatat gacacatgtg
    19801 acacctacag aactagtttt gtgtgctgtt actcatgaag atattgacca tttcatcatt
    19861 gaaactagta agccaaacgt attagttaga tggaatttag tggcacaccg tctagggtat
    19921 gaagatatta gattaaaaga ggatacagca tatgatagca cagtgcttga ccaaaaacgt
    19981 ttttaaaacg aagacaagga ggtatataaa tggctagcag tttatataat ttggctttag
    20041 atttcagcaa agaattaaac tacaccaaag ctattatggc tcgtcaaggt gataaaggga
    20101 ttacggtgac tgttaaacca tttttaaatg gcttgcagat ggatacgagt ggcggaacat
    20161 ttactttaaa aggaacaaca ccatctaacc gttatgtaga tagtgttgca actagcgtaa
    20221 ctagtgaaga agtcatattt tctcttgatg gcacatttat gagtgaagca ggatattata
    20281 aacactgcta cgtagaatat agaaaaggta atcaaatttt aacaacgcaa gacatcattt
    20341 ttttctcact aggagtgtct gacatttcgc aaggccaagc cgatgaatac gtttcgcaat
    20401 tggaagagtt gattcgaaag tataacgaaa cttttgatgc ttttatggct gaaattaaag
    20461 gtagagtgga tagcttaaat caacagatta ctgatttaac tggtcaagct aaaacgctac
    20521 aagacaagtt agatgctctg aaagaagaaa tttctaagtt aggtaactta caagtgatgt
    20581 acagcaacag catcgacttc ggtgactatg attattcggg gaacccgaat ttgttaagta
    20641 agctatcata cgacttaatt gaaaatcaaa atacttcagc tggaacactt tctaagggtg
    20701 aaaactcgtt taaatataat aagatatcag ctgaagtgga aggtggagta gagttatatt
    20761 ataaacgaag aggtatagct aactggttac cctctaataa aacgcttgta atgactgtta
    20821 agcttagagc tggagcggac tatagtccag ttgacggaaa acttatactg attagatata
    20881 ggtatgttgc cagtggaact ggcaagattg ttttagactt acctattaac agtaattcga
    20941 taactcagga atggaaagag tttagtatta ctggaactac tccaacattt agcccacaag
    21001 cataccatcc ttggatacaa tttagggctc aagatggggt acttggggaa atagaaatga
    21061 gctatgacat caaaatcgaa gaaggctcaa cagctacacc ataccagcct aatttgctgg
    21121 acgctccaca ttatttaagt aaaacaccat taggtgaaaa tattgctaat aagtctgtga
    21181 ctttcccaat taacactagc gcgtataaac tatatgaata taatacggaa gaagatctta
    21241 taataggtca aacgtatact attacgctta aaggaacaaa acctgcaagt caaacctttg
    21301 cagtgtataa ttattggaac attaatcttg gaagcttaaa accagttgag ggattgacag
    21361 acgtatggtc tataacattc acaccaacga cacttgagcc aggtttgcct aaagaccttc
    21421 gcattcttca gtcgcctaaa gaaacggccg gcgcatgcca aattgactgg ctcaagattg
    21481 aaaaaggcga cacacgaacc ccgaatattg agcaatataa ataccgagga atcggcatgc
    21541 gagactcaaa taatccaaaa gactatgtct gggatttagc accagaatat gtagaaaaca
    21601 atctggctac agatatcaaa gttgctgaaa ttactggtaa agcaaacaat tataccgatg
    21661 ggaaagtatc gcagattaat tcgcagttga ctgcttcaat taatgaagta gacaccacag
    21721 ctaagggtgc tcaaacaaaa gcgaatgcta atgcgactgc tatagatgaa ttagacaata
    21781 agatcgatga acgcattaat gatacagcta ctaccgcatt ttatggcgca caaggtgaag
    21841 tctctaacaa aacgaaagta gcggagtatg gagtaggaac agggtatact actacagctg
    21901 taggagaaac acatttcgaa cgtcaatcta acggatacgt taaatgctta aaagctggta
    21961 agtattcaat tagtgcgcaa atacgtgtgc aattgggcag gaaatatgct tcatggttat
    22021 atactgattt atataaagat ggaacttctg ttgataattt agtatcttat ggggtcgacg
    22081 ccttgcaaaa tagatttgca gctagcggaa acattgtaac tgatttagaa gtaaacgatg
    22141 tactaaatat gcgtactgaa atcggcgttg cagatggaat tttacaattt acatcttgcc
    22201 gtactttagt cttgactaga ctatctgatt aatacaatat ttataatttt gctatcaaca
    22261 cgctcaaagg agggtgtttt ttattttgca atgaaaggag gctagttggt tgaaagacga
    22321 agcaatacaa gacgtggtag aacgcttagt gcgtattgaa acgaaactgg ataattacga
    22381 attattacgt gaaaaagcgg aaagtgcaaa agatagagcg gatcaggcat attctattgc
    22441 gcttaataat gcagaagaca tcaaagagat gaaagccaat aataaatggt cgtggggtta
    22501 catgatcggt ttaggcatta caatcattgg ctatttcttg actaaattgt aaaggaggtg
    22561 agaagaaatg gttttaccag ataagtatta tcaagtcatt aaatggacag ttttaacagt
    22621 attgccagct gcatctgtat tagtagcgac gttagggaaa gcgtatggat ggaatggaac
    22681 agatatgaca gtactcacta tcaatgcagt agcaacattt ttaggtgtta tcactggtgt
    22741 gtcggcttat aatttgaaaa aataggagga aacaaatgaa aaagaaaatt actattactg
    22801 cgatgagcct attaacggct ctttttttat tgccaattaa tgggtttgcc tatactatca
    22861 acaatgaatt taatttgggc gcaaatgaag gtagctcaca agtagcaaat aatcagtaca
    22921 ttttactgca tgaaacggct aatgaaacag caacaggacg caatgaagcg cagtatatgc
    22981 aacgttcatg gactagtgct tacactgctt acattgtggg tgatggcgga attgtttacc
    23041 aagttggaca acctggttat gtacagtacg gtgctggttc gtatgctaat gccaacagtc
    23101 ctgtgcagat tgagttacaa cacacacata ataaagcaac ttttgaaaag aactataaag
    23161 catacgttga attggcgaga gattccgcta agaaatacgg tattccgctt acattggaca
    23221 cgccttataa ccaaccagga
//
LOCUS       DAXWLF010000026        22814 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXWLF010000026 DAXWLF010000000
VERSION     DAXWLF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.026"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            33..503
                     /gene="rpsG"
                     /locus_tag="ACGE4U_000787"
     CDS             33..503
                     /gene="rpsG"
                     /locus_tag="ACGE4U_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFF8392973.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            597..2684
                     /gene="fusA"
                     /locus_tag="ACGE4U_000788"
     CDS             597..2684
                     /gene="fusA"
                     /locus_tag="ACGE4U_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFF8392974.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            2856..4043
                     /gene="tuf"
                     /locus_tag="ACGE4U_000789"
     CDS             2856..4043
                     /gene="tuf"
                     /locus_tag="ACGE4U_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFF8392975.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            4450..4758
                     /gene="rpsJ"
                     /locus_tag="ACGE4U_000790"
     CDS             4450..4758
                     /gene="rpsJ"
                     /locus_tag="ACGE4U_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFF8392976.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            4789..5418
                     /gene="rplC"
                     /locus_tag="ACGE4U_000791"
     CDS             4789..5418
                     /gene="rplC"
                     /locus_tag="ACGE4U_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFF8392977.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            5446..6069
                     /gene="rplD"
                     /locus_tag="ACGE4U_000792"
     CDS             5446..6069
                     /gene="rplD"
                     /locus_tag="ACGE4U_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFF8392978.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            6069..6359
                     /gene="rplW"
                     /locus_tag="ACGE4U_000793"
     CDS             6069..6359
                     /gene="rplW"
                     /locus_tag="ACGE4U_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFF8392979.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            6398..7231
                     /gene="rplB"
                     /locus_tag="ACGE4U_000794"
     CDS             6398..7231
                     /gene="rplB"
                     /locus_tag="ACGE4U_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFF8392980.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            7274..7552
                     /gene="rpsS"
                     /locus_tag="ACGE4U_000795"
     CDS             7274..7552
                     /gene="rpsS"
                     /locus_tag="ACGE4U_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFF8392981.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            7575..7922
                     /gene="rplV"
                     /locus_tag="ACGE4U_000796"
     CDS             7575..7922
                     /gene="rplV"
                     /locus_tag="ACGE4U_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFF8392982.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            7936..8592
                     /gene="rpsC"
                     /locus_tag="ACGE4U_000797"
     CDS             7936..8592
                     /gene="rpsC"
                     /locus_tag="ACGE4U_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFF8392983.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            8595..9029
                     /gene="rplP"
                     /locus_tag="ACGE4U_000798"
     CDS             8595..9029
                     /gene="rplP"
                     /locus_tag="ACGE4U_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFF8392984.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            9019..9207
                     /gene="rpmC"
                     /locus_tag="ACGE4U_000799"
     CDS             9019..9207
                     /gene="rpmC"
                     /locus_tag="ACGE4U_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFF8392985.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            9232..9498
                     /gene="rpsQ"
                     /locus_tag="ACGE4U_000800"
     CDS             9232..9498
                     /gene="rpsQ"
                     /locus_tag="ACGE4U_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFF8392986.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            9556..9924
                     /gene="rplN"
                     /locus_tag="ACGE4U_000801"
     CDS             9556..9924
                     /gene="rplN"
                     /locus_tag="ACGE4U_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFF8392987.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            9961..10269
                     /gene="rplX"
                     /locus_tag="ACGE4U_000802"
     CDS             9961..10269
                     /gene="rplX"
                     /locus_tag="ACGE4U_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFF8392988.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            10297..10836
                     /gene="rplE"
                     /locus_tag="ACGE4U_000803"
     CDS             10297..10836
                     /gene="rplE"
                     /locus_tag="ACGE4U_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFF8392989.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            10855..11040
                     /locus_tag="ACGE4U_000804"
     CDS             10855..11040
                     /locus_tag="ACGE4U_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFF8392990.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            11076..11474
                     /gene="rpsH"
                     /locus_tag="ACGE4U_000805"
     CDS             11076..11474
                     /gene="rpsH"
                     /locus_tag="ACGE4U_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFF8392991.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            11506..12042
                     /gene="rplF"
                     /locus_tag="ACGE4U_000806"
     CDS             11506..12042
                     /gene="rplF"
                     /locus_tag="ACGE4U_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFF8392992.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            12203..12559
                     /gene="rplR"
                     /locus_tag="ACGE4U_000807"
     CDS             12203..12559
                     /gene="rplR"
                     /locus_tag="ACGE4U_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFF8392993.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            12580..13080
                     /gene="rpsE"
                     /locus_tag="ACGE4U_000808"
     CDS             12580..13080
                     /gene="rpsE"
                     /locus_tag="ACGE4U_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFF8392994.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            13095..13274
                     /gene="rpmD"
                     /locus_tag="ACGE4U_000809"
     CDS             13095..13274
                     /gene="rpmD"
                     /locus_tag="ACGE4U_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFF8392995.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            13318..13758
                     /gene="rplO"
                     /locus_tag="ACGE4U_000810"
     CDS             13318..13758
                     /gene="rplO"
                     /locus_tag="ACGE4U_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFF8392996.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            13758..15053
                     /gene="secY"
                     /locus_tag="ACGE4U_000811"
     CDS             13758..15053
                     /gene="secY"
                     /locus_tag="ACGE4U_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFF8392997.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            15115..15762
                     /locus_tag="ACGE4U_000812"
     CDS             15115..15762
                     /locus_tag="ACGE4U_000812"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFF8392998.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            15953..16171
                     /gene="infA"
                     /locus_tag="ACGE4U_000813"
     CDS             15953..16171
                     /gene="infA"
                     /locus_tag="ACGE4U_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFF8392999.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            16204..16320
                     /gene="rpmJ"
                     /locus_tag="ACGE4U_000814"
     CDS             16204..16320
                     /gene="rpmJ"
                     /locus_tag="ACGE4U_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFF8393000.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            16338..16703
                     /gene="rpsM"
                     /locus_tag="ACGE4U_000815"
     CDS             16338..16703
                     /gene="rpsM"
                     /locus_tag="ACGE4U_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFF8393001.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            16731..17120
                     /gene="rpsK"
                     /locus_tag="ACGE4U_000816"
     CDS             16731..17120
                     /gene="rpsK"
                     /locus_tag="ACGE4U_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFF8393002.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            17200..18138
                     /locus_tag="ACGE4U_000817"
     CDS             17200..18138
                     /locus_tag="ACGE4U_000817"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFF8393003.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            18166..18546
                     /gene="rplQ"
                     /locus_tag="ACGE4U_000818"
     CDS             18166..18546
                     /gene="rplQ"
                     /locus_tag="ACGE4U_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFF8393004.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            18818..19657
                     /locus_tag="ACGE4U_000819"
     CDS             18818..19657
                     /locus_tag="ACGE4U_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8393005.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            19633..20502
                     /locus_tag="ACGE4U_000820"
     CDS             19633..20502
                     /locus_tag="ACGE4U_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8393006.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            20492..21289
                     /locus_tag="ACGE4U_000821"
     CDS             20492..21289
                     /locus_tag="ACGE4U_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFF8393007.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            21492..22238
                     /gene="truA"
                     /locus_tag="ACGE4U_000822"
     CDS             21492..22238
                     /gene="truA"
                     /locus_tag="ACGE4U_000822"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFF8393008.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(22567..>22814)
                     /locus_tag="ACGE4U_000823"
     CDS             complement(22567..>22814)
                     /locus_tag="ACGE4U_000823"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393009.1"
                     /translation="KMTNLFRLSSLRAIKTTFHRFLVTEKIGNKKMIHLSTPRPQVTI
                     FLKDEKLFFEIVTCDSELNDRLTTSFLNGVQSADLRY"
BASE COUNT         7515 a         4174 c         5013 g         6112 t
ORIGIN      
        1 tatgaacaat atttcggaag gaggagttac ggatgccacg taaaggtcct gttgcaaaac
       61 gtgatgtttt accagatcct atttataact caaaattagt aactcgcttg atcaaccgtg
      121 taatggttga cggaaaacgc gggattgcag ctaacattat ctataatgcc tttgacttga
      181 tcaaagaatc tacaggtaac gatccattag aagtgtttga acaagcaatg aaaaacgttc
      241 aacctgttct tgaagttaaa gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg
      301 aagttcgccc tgaacgccgc gttactctag gtcttcgttg ggtagtaaac tacgctcgct
      361 tgcgcggtga acacacaatg gaagaacgct tagcaaaaga aatcatggat gctgcaaaca
      421 acacaggtgc ttctgttaaa aaacgtgaag acactcataa aatggctgat gccaaccgtg
      481 cgtttgctca ctatcgctgg taagattctc tctgtttggt cggcttttat aagtcgacct
      541 cagatagtgt ccttattttt agcaactaga tagtaaagag aggagaaaca aggagaatga
      601 ccagagaatt ttctttacaa aacactcgta atatcggtat catggcccac atcgatgctg
      661 gtaaaacgac tgctactgaa cgtatcttgt actatactgg taaaatccat aaaattggtg
      721 aaactcatga aggagcttca caaatggact ggatggaaca agaacaagaa cgtggtatta
      781 cgatcacttc tgctgctact acagcacaat ggaagggtca ccgaatcaac atcatcgaca
      841 caccaggaca cgtagacttc acagttgaag ttgaacgttc attgcgtgta cttgatggtg
      901 ctgtaactgt tcttgatgca caatcaggtg ttgaacctca aactgaaaca gtttggcgtc
      961 aagcaacaac ttatggtgta cctcgtatcg tatttgcaaa caaaatggac aagatcggtg
     1021 ctgacttctt atattcagta tcaactttac atgaccgttt gcaagcaaac gctcacccaa
     1081 tccaattacc aattggtgcg gaagacgact tcacaggaat tatcgacctt gtaacaatga
     1141 aggctgaaat gtatacaaac gatcttggaa cagagatcga agaaacagaa attcctgaag
     1201 aataccgcga attagctgaa gaatggcgcg aaaaattggt tgaagctgta gctgaaactg
     1261 atgaagaact tactttgaaa tacttagaag gtgaagaaat cactgaagct gagttaaaag
     1321 aaggtatccg ccgcgcaaca gttaacgtag aattctaccc agtactatgt ggttcagcat
     1381 ttaagaacaa aggggttcaa ttactattgg acgctgttct tgattacttg ccatcaccgt
     1441 tagatatccc agctatcaaa gggattgacc ctaaaactga tgaagaagta gaacgtcctg
     1501 cagacgacag cgctcctttc tcagctttag catttaaagt tatgactgac ccattcgtag
     1561 gtcgtttaac tttcttccgt gtgtactctg gtgttttaca atcaggttca tacgtacaaa
     1621 acgcaacaaa aggcaaacgt gaacgtgttg gtcgtatcct acagatgcat gccaactctc
     1681 gttcagaaat ctctgaagtt tacgctggag atatcgctgc tgctgttggt ttgaaagaca
     1741 caactactgg ggacacacta tgtgatgaaa agagtctagt tattcttgaa tccatggaat
     1801 tcccagaacc agttatccaa gtcgctattg aacctaaatc aaaagctgac caagataaaa
     1861 tgggtgtggc tttacaaaaa ctatctgaag aagatcctac tttccgtgct gaaacaaacg
     1921 tggaaacagg cgaaacaatc atcgctggta tgggtgaact tcacttagat atcatcgttg
     1981 accgtatgcg tcgtgaattc aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc
     2041 gtgaaacttt ccgtgctggc actcaagccg aaggtaagtt tgtacgtcag tctggtggta
     2101 aaggtcaata cggtcacgta tggattgaat ttacaccaaa tgaagaaggt gctggtttcg
     2161 aatttgaaaa tgcgatcgtc ggtggggttg ttcctcgtga atacattcca gctgttgaaa
     2221 ctggtttgaa agatgctatg gaaaatggtg tcttagctgg ctatccatta gtagatatca
     2281 aagctaaact ttacgatggt tcataccatg atgtcgattc aaatgaaact gccttccgtg
     2341 ttgctgcttc tatggcatta cgtgcagcag ctaagaaagc taatccagta atccttgaac
     2401 caatcatggc tgtagaagtt gttatccctg aagattattt aggtgatgtt atgggtcacg
     2461 taactgctcg tcgtggacgt gttgaaggta tggaagctcg cgctggcggc caacaagttg
     2521 ttcgtgcgat ggttccactt gctgaaatgt ttggttatgc tacaactctt cgttctgcta
     2581 ctcaaggtcg tggtacattt acaatgacat ttgaccacta cgaagatgta ccaaaatcag
     2641 tacaagaaga aatcattaag aaaaacggcg gaaaagctga ataatttctt aaaaccggat
     2701 agtcctgcaa agagattatc caaagagact atatttttaa tatagtttcg tgtaaaataa
     2761 ttatcgatga tatgggcatg tggtgtacct tgtgtacatc atatacctat cagacaaata
     2821 aaaaatatat tgtattttta ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt
     2881 ctaaaccaca cgttaacatt ggtactatcg gacacgttga ccatggtaaa actacattaa
     2941 cagctgcaat cacaactgta ctatctaaga aaaacggcgg ccaagctatg gcttacgatc
     3001 aaatcgatgg tgctccagaa gaacgcgaac gcggaatcac aatctcaaca gctcacgttg
     3061 agtatgaaac agatacacgc cactacgctc acgttgactg cccaggacac gcggattacg
     3121 ttaaaaacat gatcactggt gctgctcaaa tggacggagc tatcttggta gtttctgctg
     3181 ctgacggccc aatgcctcaa actcgtgaac acatcctatt gtctcgtcaa gttggtgttc
     3241 cttacatcgt tgtattcttg aacaaagtag acatggttga tgacgaagaa ttactagaat
     3301 tagttgaaat ggaagttcgt gacctattaa cagaatacga attccctggt gacgatgttc
     3361 ctgtagttgc tggatcagct ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa
     3421 ttcttgaatt aatggctgca gttgacgaat acatcccaac tccagaacgt gacaacgaca
     3481 aaccattcat gatgccagtt gaagacgtgt tctcaattac tggacgtggt actgttgcta
     3541 caggtcgtgt tgaacgtgga caagttcgcg ttggtgacga agttgaagtt gttggtattg
     3601 ctgaagaaac ttcaaaaaca acagttactg gtgttgaaat gttccgtaaa ttgttagact
     3661 acgctgaagc tggagacaac attggtgctt tactacgtgg tgttgcacgt gaagacatcc
     3721 aacgtggaca agttttagct aaaccaggta caatcacacc tcatacaaaa ttctctgcag
     3781 aagtatacgt gttgacaaaa gaagaaggtg gacgtcatac tccattcttc actaactacc
     3841 gtccacaatt ctacttccgt acaactgacg taacaggtgt tgttgaatta ccagaaggaa
     3901 ctgaaatggt tatgcctggc gacaacgtaa caatggaagt tgaattaatc cacccaatcg
     3961 ctatcgaaaa cggtactaaa ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg
     4021 tcgttacaga aatcaaagct taattcactt aagttttaaa gtaaagcaac atttattcgg
     4081 acgtaatcgc tcggaacgaa ggtcttagga tctttgttcc gagctttttt atttattgca
     4141 ggatcaggaa aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt
     4201 attttctaaa tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg
     4261 tgagtttata tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta
     4321 atcagaatga tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca
     4381 cgaacgagcg tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga
     4441 gggaacaaga tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt
     4501 ttagatcaat cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct
     4561 ggtccaattc cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa
     4621 tacaaagatt cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg
     4681 aacccaacac caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac
     4741 atcgaaatca aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga
     4801 atcttaggga aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca
     4861 gtaactgttg tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat
     4921 ggctacgaag ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct
     4981 gcgaaaggtc atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa
     5041 aatgttgagc taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca
     5101 ggagacatta ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa
     5161 cgccacggac aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg
     5221 tcaatgggtc cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg
     5281 ggtggcaacc gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat
     5341 gttatcttga tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca
     5401 gctgtgaaag ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca
     5461 ttattcaaac aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga
     5521 attgaaccta atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga
     5581 caaggaacac acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg
     5641 cgtcaaaaag gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga
     5701 ggtggcgtag tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt
     5761 cgtcgtttag caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca
     5821 atcgaaggct tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac
     5881 ttatctatcg actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta
     5941 tcagcacgta acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac
     6001 gttgtatcaa atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg
     6061 cttgcataat gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc
     6121 ttgccatgga tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag
     6181 taaaacaagc ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg
     6241 tacgtgcgaa attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag
     6301 cgatcgttac attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag
     6361 cactaataga taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc
     6421 tacctcaaat ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac
     6481 acctgaaaaa tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg
     6541 catcactgta cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa
     6601 acgtaacaaa gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc
     6661 agctaacatc gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa
     6721 aggactagaa gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa
     6781 tgctcttcca ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc
     6841 tggtaaaggt ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga
     6901 aggcaaatac gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg
     6961 ccgtgctaca atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc
     7021 tggacgctct cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa
     7081 tgatcaccca cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc
     7141 tccttggggt caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa
     7201 acttattgtt cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga
     7261 gaggaggttc accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat
     7321 gaaaaaagtt gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg
     7381 ccgttcaaca gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa
     7441 acatgtacca gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc
     7501 tacaagaact tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg
     7561 gaggataaat ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc
     7621 gcacttcacc tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag
     7681 atgcaatttc aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat
     7741 tgatgtcagc agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag
     7801 taagcgaagc ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag
     7861 gatcagcttc tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat
     7921 aaggagggat aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc
     7981 cgcgactggg atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat
     8041 ttaagaatcc gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa
     8101 atcgaacgcg caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt
     8161 attggtaaag gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt
     8221 aaaagaatcc atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta
     8281 ggtgaaggaa tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa
     8341 gccatccaac gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt
     8401 ctaaacggag cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat
     8461 actttacgtg cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta
     8521 ggagttaaag tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa
     8581 ggagggaaat aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa
     8641 aaatgcgcgg ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag
     8701 ctgttgattc acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc
     8761 gttacatgaa acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg
     8821 ccaaagcaat tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac
     8881 cagtaaaacg tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg
     8941 aagcgttacg tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg
     9001 aaatgggtgg tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg
     9061 cttgatcaag aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca
     9121 ggtcaattag aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa
     9181 actgtgttgc gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa
     9241 ggaagaaatc aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca
     9301 attaccgttg ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat
     9361 tctaagaaat acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga
     9421 attatggaaa ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa
     9481 gaagcagtta ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa
     9541 aggaggatac acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc
     9601 gcacgtgaaa ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc
     9661 ggtgatgtga tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt
     9721 gacgtcgtta aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca
     9781 tatattaaat ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga
     9841 acacgtatct ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt
     9901 tcactagcac cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt
     9961 atgtttgtta aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa
    10021 ggcgttgtac tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc
    10081 gttaaaaaac accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa
    10141 gcgccaattc atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt
    10201 ggctacaaag aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta
    10261 gataaataaa agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat
    10321 cttaaagaag taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca
    10381 cctaaagttg aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa
    10441 aacttagata aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca
    10501 aaagctaaaa aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa
    10561 gttacattgc gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta
    10621 ccacgtgtac gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat
    10681 actttaggta ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa
    10741 gtacgtggta tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag
    10801 ttgttgacac aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct
    10861 aaaaaatcaa tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact
    10921 cgttgcgaac gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt
    10981 tgcttccgcg aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa
    11041 aaagtaaatt cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag
    11101 attttctaac tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg
    11161 catcaaaaat taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg
    11221 ttgaatacat cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa
    11281 acggcgaacg tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg
    11341 tcaaagctga cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt
    11401 ctgaaggtgt tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg
    11461 cttacgtatg gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga
    11521 aataaagtcg ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca
    11581 gttaaaggac caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc
    11641 gaaggaaacg aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat
    11701 ggaacaactc gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa
    11761 gcgttagaac taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg
    11821 aacgttggtt actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta
    11881 ccagctaaca cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct
    11941 gcaaacattc gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt
    12001 ggcgaatatg tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca
    12061 tagaagccag cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt
    12121 ttacttgtgc cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa
    12181 taaaaataaa gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa
    12241 gagacatcgt cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt
    12301 ttttcgttct aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct
    12361 agcaagtgcc tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca
    12421 agctgtcggt aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt
    12481 tgaccgtggt ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga
    12541 aaatggacta gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa
    12601 catttggaat tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt
    12661 ggacgtcgtc tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtaggg
    12721 tttggtacag gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg
    12781 aagaaaaact tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt
    12841 gcttttagtg gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct
    12901 ggtggaccag ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct
    12961 ttaggctcaa atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg
    13021 aaacgtgcag aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa
    13081 ggaggacaac aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc
    13141 ctcaaaacca acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg
    13201 ttaagccttc taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg
    13261 ttgaagaaat ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg
    13321 aaacttcatg aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt
    13381 ggtacttcat ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt
    13441 tcaggcggcg gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta
    13501 ccaaaacgag gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta
    13561 ttgaaccgct ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc
    13621 gtgaaaaacg aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta
    13681 actgtgaaag cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga
    13741 tcaatcgagg tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta
    13801 gatcgaaaat cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta
    13861 ctgtaccagg cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca
    13921 tgttgaactt ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt
    13981 caccatacat tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat
    14041 ttgttgaatg gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat
    14101 atctgacatt agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat
    14161 acgctcaatt aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga
    14221 ttctaacagc cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta
    14281 tcggaaatgg cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta
    14341 tcagagaaat ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg
    14401 ctatttttgt ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt
    14461 tccaacaagc tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta
    14521 caagcagcta tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta
    14581 gttcgtttat tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg
    14641 aaaactggta caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga
    14701 tttatacgat tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg
    14761 agaaattagc ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta
    14821 aggggactga agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct
    14881 tccttggttt agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat
    14941 caatcggatt aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga
    15001 aacagttaga aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg
    15061 tgttgaggtt tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac
    15121 ctcattttaa tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat
    15181 gcttatggta ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa
    15241 acagctcttg gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa
    15301 gtaacaaatg gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta
    15361 ttagatggct ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat
    15421 ttgaataaaa aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa
    15481 cgtctagccg gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac
    15541 cctacaaaag ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa
    15601 gatgataaac ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca
    15661 atcttggctt attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc
    15721 gatgctgtat ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg
    15781 acttgcatta tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc
    15841 gacttctaga gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt
    15901 tccgccattt tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa
    15961 agaagatatg attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa
    16021 agttgaacta gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca
    16081 ttacatccgt atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa
    16141 ccgtggccgt attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt
    16201 atcatgaaag taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt
    16261 aaaggacgcg ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa
    16321 ttggaggtgc tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg
    16381 tagtagtttc acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag
    16441 ctgatgctgg tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg
    16501 ctatccgtgc agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact
    16561 tgaacatcaa acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat
    16621 tgcctgttcg tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg
    16681 ctatcgctgg caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa
    16741 aaaaagtgaa tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc
    16801 attctacatt caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt
    16861 ggtcatcagc tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc
    16921 aaatggcagc agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag
    16981 taacagttaa aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag
    17041 gtttagaagt gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc
    17101 caaaacgccg tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg
    17161 aacaagacac ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca
    17221 agaatcgcaa aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa
    17281 agagggtatg ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca
    17341 ggtgctgcca tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa
    17401 ggtgttagag aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg
    17461 tatggccaag aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct
    17521 ggcgatatta tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt
    17581 gtctctgaag gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt
    17641 caagcagatg aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc
    17701 tacacacctg ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa
    17761 gattttgata aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca
    17821 atgagtttgt ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat
    17881 gaagcaaaaa atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg
    17941 gaaatgacga tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca
    18001 ggaatcaata ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt
    18061 aacttaggac gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt
    18121 ttacgtaaag aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa
    18181 ctaggacgca catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta
    18241 atcaacgaac gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa
    18301 aaaatgatca ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc
    18361 gtacgtaatg aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct
    18421 ttacaaaaac tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca
    18481 cgcattttga agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt
    18541 gtctaaaatc acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt
    18601 ggaagatatt tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag
    18661 taagcactca tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt
    18721 tgaaggcata aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata
    18781 gtgttagtag gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga
    18841 aaagataaat tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt
    18901 tacaattgat aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac
    18961 acttgccaaa acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa
    19021 tcaaatattg gatgaagaaa atatttggac gatccgacag atggttggaa tggtttttca
    19081 aaatccagac aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga
    19141 aaatcaaggg attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt
    19201 tagaatggct gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg
    19261 tgttgccatt gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac
    19321 cagtatgcta gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga
    19381 agaaagtcaa ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa
    19441 tcggatacta gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt
    19501 ttctgctggt cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa
    19561 gagcgcactg aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat
    19621 ggtggattgg ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca
    19681 ccatttgaac aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact
    19741 gcattagtag ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt
    19801 gttaagccaa caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat
    19861 aataagaatt taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca
    19921 cagctattcg aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc
    19981 agcgaagaag aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa
    20041 agctatttgg aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc
    20101 gctggtgtgc ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt
    20161 gatccccagg gtagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag
    20221 atcactattg tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc
    20281 tatgtgttgg aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac
    20341 attgaatggc taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa
    20401 ttaatggcta aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac
    20461 gctatcgtcc attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta
    20521 tatgcctgga gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt
    20581 ttattttatc ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt
    20641 tttcacatta ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat
    20701 tcgcccattg atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg
    20761 cggagaaact tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg
    20821 cctctttatt ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac
    20881 tacaccgcct ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt
    20941 acggttccca gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac
    21001 attgatggac gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg
    21061 tgaaggaaac ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt
    21121 cagcagcttc aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg
    21181 tggggaagga agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat
    21241 cattgctttt gtattattaa ctgtgctact gatctttttg agaggataaa cgttcagtgg
    21301 gccgctatct attcttttct tgctgatctg tttgcaagaa aggatgcgag cagtgtatga
    21361 caagaaatat tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa
    21421 gaaagaacga aaaaatttta taaaaaaatc attattccag ataacctttt aggaacaaag
    21481 gagagaaaaa catggtacgt tacaaagcaa tcattgcata cgacggaaca aattttaatg
    21541 gattccaaaa acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa
    21601 aaatggcaaa tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac
    21661 atgcgattgg gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc
    21721 gtttcgcgct agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt
    21781 cagaagactt ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata
    21841 tagggaaacc acgcagtcct tttcgacgcc attatgcaag ttattttcct taccctcttg
    21901 atttggaaaa aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt
    21961 tttgtgcatc aggaagctcg atagaggata aagtacgtac gatctacgag gctaagatgg
    22021 aggtaaatga aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata
    22081 agatgatacg gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag
    22141 atagtatccc agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacatc
    22201 ctgaaggact ttatttgtat gaagtgaaat atgaataaga ggttgtgaca gaagtggtca
    22261 gcttcaagaa ataagaagga atttacgaaa attacttctc aaaattttgc aaaatttcgg
    22321 cttattttcg aaggagctac ttctggaaca ccgtgaatca gaggttgtga aaaagctgcc
    22381 ctgcatcaag taataagaac aaccaaacaa aaatagctcg tcatattttt cgtttggttg
    22441 ggcttattac cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag
    22501 cgttttgacc tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt
    22561 gttgagctaa taccgaaggt cagctgattg aacaccgttt aagaaagagg tcgtgagcct
    22621 gtcatttaac tctgagtcac aagtaacaat ttcaaaaaac agcttctcat ctttgaggaa
    22681 tatcgtgact tgtggacgag gcgttgataa gtgaatcatc tttttgttac ctattttttc
    22741 tgtaaccaaa aaacgatgga acgttgtttt gattgccctt aatgaggaca gtcggaacag
    22801 gtttgtcatt ttta
//
LOCUS       DAXWLF010000027        22672 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXWLF010000027 DAXWLF010000000
VERSION     DAXWLF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.027"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            83..679
                     /locus_tag="ACGE4U_000824"
     CDS             83..679
                     /locus_tag="ACGE4U_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFF8393010.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            660..1106
                     /locus_tag="ACGE4U_000825"
     CDS             660..1106
                     /locus_tag="ACGE4U_000825"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFF8393011.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1449..1772
                     /gene="relB"
                     /locus_tag="ACGE4U_000826"
     CDS             1449..1772
                     /gene="relB"
                     /locus_tag="ACGE4U_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFF8393012.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            2092..2319
                     /locus_tag="ACGE4U_000827"
     CDS             2092..2319
                     /locus_tag="ACGE4U_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFF8393013.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2335..3501
                     /gene="hemW"
                     /locus_tag="ACGE4U_000828"
     CDS             2335..3501
                     /gene="hemW"
                     /locus_tag="ACGE4U_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFF8393014.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3736..4779
                     /gene="hrcA"
                     /locus_tag="ACGE4U_000829"
     CDS             3736..4779
                     /gene="hrcA"
                     /locus_tag="ACGE4U_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFF8393015.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4873..5436
                     /gene="grpE"
                     /locus_tag="ACGE4U_000830"
     CDS             4873..5436
                     /gene="grpE"
                     /locus_tag="ACGE4U_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFF8393016.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5487..7316
                     /gene="dnaK"
                     /locus_tag="ACGE4U_000831"
     CDS             5487..7316
                     /gene="dnaK"
                     /locus_tag="ACGE4U_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFF8393017.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7467..8633
                     /gene="dnaJ"
                     /locus_tag="ACGE4U_000832"
     CDS             7467..8633
                     /gene="dnaJ"
                     /locus_tag="ACGE4U_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFF8393018.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(9001..9738)
                     /locus_tag="ACGE4U_000833"
     CDS             complement(9001..9738)
                     /locus_tag="ACGE4U_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8393019.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9740..11242)
                     /locus_tag="ACGE4U_000834"
     CDS             complement(9740..11242)
                     /locus_tag="ACGE4U_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFF8393020.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11501..13495
                     /gene="uvrB"
                     /locus_tag="ACGE4U_000835"
     CDS             11501..13495
                     /gene="uvrB"
                     /locus_tag="ACGE4U_000835"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFF8393021.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            13505..16321
                     /gene="uvrA"
                     /locus_tag="ACGE4U_000836"
     CDS             13505..16321
                     /gene="uvrA"
                     /locus_tag="ACGE4U_000836"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFF8393022.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16584..17849
                     /locus_tag="ACGE4U_000837"
     CDS             16584..17849
                     /locus_tag="ACGE4U_000837"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFF8393023.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            17853..18584
                     /locus_tag="ACGE4U_000838"
     CDS             17853..18584
                     /locus_tag="ACGE4U_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFF8393024.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            18719..19603
                     /gene="rapZ"
                     /locus_tag="ACGE4U_000839"
     CDS             18719..19603
                     /gene="rapZ"
                     /locus_tag="ACGE4U_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFF8393025.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            19600..20598
                     /gene="yvcK"
                     /locus_tag="ACGE4U_000840"
     CDS             19600..20598
                     /gene="yvcK"
                     /locus_tag="ACGE4U_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFF8393026.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            20632..21567
                     /gene="whiA"
                     /locus_tag="ACGE4U_000841"
     CDS             20632..21567
                     /gene="whiA"
                     /locus_tag="ACGE4U_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFF8393027.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            22135..>22672
                     /locus_tag="ACGE4U_000842"
     CDS             22135..>22672
                     /locus_tag="ACGE4U_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393028.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELL"
BASE COUNT         7552 a         3820 c         4895 g         6405 t
ORIGIN      
        1 taacttgaca caaaatgagt ttttttcgat ttttcaagtt atctaatgca attatattga
       61 tatcattagg agctgcattg ttatgaatcc aacattgatt actaagaaag aattactaaa
      121 aaaattagat atttctactg gtgtattagc taatttaatt agaaatggca tgccaaaaga
      181 gggagaaatg tttaatcttg ataagattat tacatggcga gaaaattggt ctaaaaacat
      241 attaggtgag ttagaagttg gtagagtata tactaacaaa gaaatttcag agaagtttaa
      301 atgttccaaa cagggtggaa tgagaagatc tcatcaaact aatactcttg tactgtttag
      361 tgatcaaaca ggaagtaatg tttataaaga taagtggtta aatggtattt tacaatatac
      421 cggtatgggg ctaaaagggg atcaagtatt ggataagaat caaaataaag ttttagccaa
      481 ttcaaaaagt aattttgtaa agattcattt atttgaaaca tttaaaccaa aagaacacac
      541 atatctggga gaagtatatt tagcaggaca aatatatact gttaatgaaa aagatagttc
      601 gggcaacagc agaaaagtat ataaatttcc attagcttta ataaaccaag agcaattgat
      661 tgaagataaa gatatataac caggaagaaa atcaaactag acatattcgt aatttatcta
      721 atgctaaatt agaggaagaa gcaagaaaag ttagtaatta taataaaaaa atggctgcaa
      781 aatatgttaa cgaagaggag ttttgcttct caaaaacaaa aatatataaa cgtaatccct
      841 tcgtatctga atatgttaaa cgacttgcaa aaggtatttg tcaactttgc caagaaaaag
      901 ggccatttat aaaagacggt gtaccttatt tacattgtca tcatatagaa tatttgtcgc
      961 aaggtggaaa agatgtgatt gaaaactgta tcgcgttatg tcccaattgt catgctagaa
     1021 ttcatgaact tgagttacct gacgataaag aaaaattgct gaagatcgtt gaaaaaagag
     1081 aaaatagctt ttctaaaata ccgtaattta tttttatagg acaaataagc taatgtgagc
     1141 tatgccttct atataagact aagttctatt ataagtaacg aagtaattct cgttccttcc
     1201 tgtagtaata ttttttaaat aacctatagg cttcttcaaa agtacatcct ccagtaaatt
     1261 ctaagtactg gaggattctt tttctagtgt tgctttactc atgaaaaagt agttggctac
     1321 ttcatcaaaa tttctgaact tttttaatgt ataataaaga gctcgatcat ttactacaag
     1381 gatgctagct cctacatttt cacagttttt tagcaagtaa atttttctaa aatcacatca
     1441 ggaaatgcat gagattcttt aagagaggca attatagggc tatgattacg gttcgtgtaa
     1501 gtgacgccaa aaaagaatgg ctttcttata tggctgaatt ttatgggatt actttatctg
     1561 aacttctaaa aaactattcg atggaacaac tagaagatga atacaatcgt caaacggctg
     1621 aaattgccca taaacattgg ataagaagtg ggaaaacaac agtattcatg gaagaatcct
     1681 taatgaattt ggtgggctgg aatgacctgc cgtttaaaat tcacgcccga aattcagaag
     1741 cagctctgat tggcaaatac gtgtattggt agagatagaa gataatcaat tcgttgttat
     1801 cgctattcaa gtagggcacc gaagaaatgt ctatgattaa gagactgttt atttttacaa
     1861 aaaaataata attggaatct agttaaaact cttgaagtga gagatttgac tagatttttt
     1921 tatgaataaa ataaggacaa aattgtttag gaagaatcat gagtgtaaaa aaatcatcca
     1981 agaagtcgat tcttggattt tttagtggat ttgttcgcca aatacgttta gtatgcgtat
     2041 atgagaggtt gtaagcaaag cgttgtggtc tgagcactta aggaggaatc tgtgaaaata
     2101 gcttttcata tttttgcaga ttttgactta atgccgaagg tcagctttgc gattaccgtt
     2161 tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc atttttagag
     2221 ctaggtttcc ttgattctaa atatgcggct tatttcaatg agttggtgct tgaacgcgat
     2281 tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat ttcaatgata
     2341 gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc attttgcgag
     2401 catatctgct attactgtga ctttaataaa gtttttttag aagggcagcc agtagatgag
     2461 tatgtggaaa tgctgctgaa tgaaatgaga ttaatgctgg aaagtcatcc tacagcgaat
     2521 ttggaaacac tttatgtagg aggcggaaca ccaacttctt tatctgctaa acaacttgat
     2581 cgactattat caggtgctag agagatcctg ccttttcaaa atggtaatga gtttactgta
     2641 gaagcgaatc ctggtgattt gacccgtgaa aaacttcaag tgatgaaaaa ctatggcgtc
     2701 aatcgtttat ccatgggagt gcagacattt gacaatcgct tattgaaaaa aattggacgt
     2761 aaacatactg cagaagatgt gtatcaaaca atgcaatttt tagaagcaga aaattttacc
     2821 aatgtaagca tcgatttgat ttatgcatta ccgggacaaa cactagaagg atatcgggat
     2881 actttggaaa aagctttggc tttggatctg cctcactatt ctttgtactc attgatcctc
     2941 gaaaacaaaa caatgttcat gaactgggta cgacaaggaa gactcgaact gcctgatcag
     3001 gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa tggaaaagca tggacgtcac
     3061 caatatgaaa taagtaactt tgggctctcc ggacatgaat ctcaacataa cctcatgtac
     3121 tggaacaatg accactattt tgggtttgga gctggtgcta gcggttatct tggagatatt
     3181 cgttaccgca acaaaggacc aatccaacac tatttacgcc cattacgagc aaatgaacgc
     3241 ccggtattgg aagaagaaaa attgacacga aaaaatcaaa tagaagaaga aatgttctta
     3301 ggactaagaa aaaaaacagg gattactaaa aaacatttct atgatcgcta tcaaaaaaca
     3361 attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt acttgttaat
     3421 gaagaagacc gtgtttattt gacaccaaga gggacttttt tggggaacaa tgtgtttgag
     3481 cgctttttat ttgaaaaata gtttgaagaa tatttgtttt ttatcgaaac ttgaaaagaa
     3541 aaagtttaga aagtttgaca atgaggtatt gaagtattga tataactgga tttacatcaa
     3601 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
     3661 ggagaagggc ttcttgctat atttataagt gaattagcac ttagaatata tgagtgctaa
     3721 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
     3781 cactatacga gtagtggcgt gcccgttggt tccaaaacat taatgtctga gggagtcaag
     3841 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
     3901 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
     3961 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
     4021 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
     4081 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
     4141 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
     4201 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
     4261 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
     4321 cttcgtacgg agattccgat gattcttcat aaatatttcc agacgacaga agggatgtct
     4381 cagttatttg acaccgttct tggtcatgtt tttgaagaaa aggtttatgt cagtggacaa
     4441 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
     4501 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
     4561 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
     4621 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
     4681 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
     4741 gctgattact accgctcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
     4801 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
     4861 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
     4921 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
     4981 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
     5041 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
     5101 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
     5161 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
     5221 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
     5281 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
     5341 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
     5401 gtgttaagac caactatggt cattgttgca caataattgt gcggatataa aacttacact
     5461 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
     5521 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
     5581 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
     5641 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
     5701 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
     5761 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
     5821 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
     5881 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
     5941 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
     6001 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
     6061 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
     6121 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
     6181 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
     6241 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
     6301 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
     6361 gtcaagcgtt aaaagatgca ggtctgtcac aatcagaaat cgacgaagtt attttagtcg
     6421 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
     6481 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
     6541 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
     6601 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
     6661 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
     6721 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
     6781 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
     6841 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
     6901 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtgaaa gatgcagaag
     6961 caaacgctga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagacg
     7021 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
     7081 aagtgaaaaa agcagaaaca gctcgtgacg aattaaaagc cgctgttgaa gcaaacaaca
     7141 tcgaggaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
     7201 aactatacga acaagctgca caacaacaag ctcaagaaaa tccagaagca gctcaaggcg
     7261 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
     7321 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
     7381 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
     7441 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
     7501 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
     7561 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
     7621 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atacgaccaa tacgggcatg
     7681 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
     7741 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
     7801 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
     7861 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
     7921 aaatctgtca tacttgtggt ggaaacggtg caaaaccagg cacacaacca actacttgtc
     7981 ataaatgtca tggtgcaggt acgatcaatg tagaacgcca aacgccactt ggacgagtga
     8041 tgagccgtca aacttgtgat gtttgtcatg gcacagggaa agaaatcaaa gaaccttgtc
     8101 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
     8161 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
     8221 gcggaccatt tggtgatttg tatgtggttt ttcgtgtaga agaaagtgat atctttgatc
     8281 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
     8341 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
     8401 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
     8461 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
     8521 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
     8581 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
     8641 agtaaaaata taaagtagac ttgcccaaat tttctatttc tagtaaaaac gctaagtcat
     8701 taataattaa aaaccagacg aacaatcata cgttcgcctg gtttttttgt agttgtcatg
     8761 tttgtaccaa caaaaaagcc agctttagaa taagataatc atcatagttt ctgaaggtgg
     8821 tatcgactat gtacttgata tcgtcaatga accgatggta ctttcttatg cgatggaaca
     8881 ggtagagcga ttgcttgttt gtgcaggaga acaagcgatg cggctggtac agttaagtga
     8941 aaaaacaaga aactaaaaat gcccggtcgt catgaagcga ccgggcattt ttggttttta
     9001 ttaaatattt aatactttat tcaagaaatc ttttgtccgt tcattttgcg gattttcaaa
     9061 gatttgttga ggcgttccgt cttctaaaaa gtttccgtcg tcaatgaaca tgacacgatt
     9121 ggccacttct ttagcgaagc ccatttcgtg cgtaacgata accatcgtca taccttgttc
     9181 tgctaacttt ttcatgacgt tcaagacatc accgaccatt tcggggtcga gtgcagatgt
     9241 aggctcatca aaaagcatga tgtcagggtt catcgctaga gcacgagcaa tagccacacg
     9301 ctgcttttgt ccaccggaaa gcatttcagg atacatatct tttttatctt ctagaccaac
     9361 tgtctgcagc aatttgattg cttttttctc tgcttcttct ctagattctt tcttcaggtc
     9421 catcggtgct aaaatgatgt tttccaaaat agaaagatga gggaataggt taaagtgctg
     9481 gaataccatt ccgatatgtt gtctcaccaa gttgatatct gtgcttttat ccatcaagtg
     9541 ggcgccgtca ataataattt caccgtcaga aggttcttct aaacgattca agcaacgcaa
     9601 aaatgtactt tttcctgaac ctgaagggcc aatgatacag acgacatctc cttcgttgat
     9661 ggacacattg atgtcgttta atactgtgtt atcgccgtat tttttgacta aatgctcaac
     9721 taagattttt tctgccatct tacttcacct tcttttctaa aacttttgct aattttgtca
     9781 atgctgtgat caaaatcaag tacatgatcg caatgatcaa gtaaacatac gtactttgtg
     9841 ttgtacgtgc aacaatgatc ttaccagttt gcagcaattc gactacaccg attgcggaaa
     9901 tgatcgttgt gtcttttaat gagataacga actgattgac gaatgatggg atcatgatgc
     9961 gtatcgcttg cggcaaaatg attttttgca ttgtccgatt ataagaaaga cctaaacttc
    10021 tggaagcttc catttgtccg acaggaacgg cgttgatccc gcctcgaaca atctctgcga
    10081 tataagcact tgcgttaaga gtcaaggtga taacaccagc aaggaagtca ggaatcgtaa
    10141 atccaacgat accaggcaac ccaaagaaaa taaagaacgc aagaaccatc attggaattc
    10201 ctcggatgat atcgacataa atactagcaa ttgtgcgaag agcgcgtatt ggtgctacac
    10261 tgaataatcc aaaaatcaca ccgattacta tagccaaagc aaaggagatc aaagttagga
    10321 gaattgttct ccaaagtcct ttcaacaatg cttgatagtt gttttggata agtccaagaa
    10381 atgttgattc atcagcagtg gaagactcac tggaagtttc tgaaccatcc gctacatatt
    10441 ctttaataat cttatcgtat tcgcctgttc tttttaattc tttcaatcct tcattgaaca
    10501 tattaagaag ctcaggattt tgtccttttt taacggcaaa tgcatatttt ccgccagatt
    10561 cttttttgat cggtgtttcc aattcttgtc cttgtttgat cgcataaccg attaccggat
    10621 aatcatccat cattgcatcg attgcgccaa cttttaaccc gtcgtacaat tgatcagcag
    10681 catcaaaata tttgatagaa tagccatatt cagattcatg gtcgcttaaa aattcagcac
    10741 tttcggtacc aacttttgct ccgactgttt ttcctttcaa atcttcataa gacttgatcg
    10801 aagtatctcc ttttttgaca gctaattgga tcccgctatc aaagtaagaa tcagaaaaat
    10861 catagctttt ctttcgttca tctgttacag tcattccggc aatcattccg tctgcttgtc
    10921 ctgattccaa agcttgcaaa gcagcagaga aaccacggta gtccattcgg atgttaaatc
    10981 cttgcagttt ggcgatgttt tccatcaatt ttacatcgat gccttcgtac tcgccctttg
    11041 cgttttgaaa ttcaaatgga gcaaaagcag agtcactggc aattacgtat acgtcttttt
    11101 tgggctctat tttttgcatt gtctcgcttg tgcttgactc tgccgttgta gtgttgtcag
    11161 cttgtgcaac atgtccaaac gcaagtgtca caagtcctgt cattaagaaa acaaatgagt
    11221 gaaataaatg ctttcttttc ataatcaacc tcctaaattt ccaatatttt attattatag
    11281 gcgaaaagtg ctgaatcaga caagactgta agctcgaaaa aatgaaaaaa atgcgagttt
    11341 ctaacatttt atgttataaa acgtaacatg cataataaga taaaaaagtg gcgaaagtat
    11401 gttcggtgtg gtataatttt ttagtaagga atgcctttgc tttccttttc tttttattgg
    11461 tcgtaaaatg aaaaggaatg aatgaaaagg aggacgaaga atgattgaga gagatacctc
    11521 ccgacacttt gagcttgtct cgaaatacca accggctggt gatcagcctg aagcgattaa
    11581 ccaattagtg gatggcgtag tcggcgggaa aaaagcacaa atcttattag gtgcgactgg
    11641 aacagggaaa acatatacga tttccaatct tattgagaaa gtgaataaac caacactaat
    11701 tattgcccat aacaaaacat tggctggtca gctgtacggg gaattcaagg aatttttccc
    11761 taacaatgct gttgaatatt ttgtttctta ttatgattat tatcagcctg aagcttatgt
    11821 gccttcaagc gatacgtata tcgaaaaaga ttcaagtgta aatgatgaga tcgacaaatt
    11881 acgacattcg gctactagtt cattattaga aagaaatgat gtgatcgtca ttgcatccgt
    11941 ttcatgtatt tttggattag gttcgccttt tgagtaccaa aaacaagtag tttcgattcg
    12001 acagggagca gaactagatc gcaatcagtt gattcgtgac ttagtcagta tccaattcga
    12061 aagaaatgat atagattttc agcgcggtcg ttttcgagtg cgcggtgatg tcgtggagat
    12121 ttttccggct tcaagagatg aacgtgcctt acgtgtggag ttttttggag atgaggtcga
    12181 acgtatccga gaagtcaatg cactgacagg tgaagtttta ggagaaacgg aacacgtggc
    12241 aatcttcccg gcgactcact ttgtgacgaa tgatgaacat atggagcatg ccgtagcgaa
    12301 tatcaaagcg gaattagaac agcgtttaac tgtcttgcga aatgaaaaca aacttctgga
    12361 agctcaacgt ttagaacagc gaaccaatta cgatattgaa atgatgttag aaatgggcta
    12421 cacgtcggga attgaaaatt attctcggca tatggacgga cgtaaagaag gtgaaccgcc
    12481 ttatacgcta ttggatttct ttcctgaaga ttttttgatc gtggcggacg aatcgcacgt
    12541 aaccatgcct caagtcagag gaatgtataa tggtgaccga gcgagaaaac agatgctagt
    12601 agattacggt ttccgtttgc catctgcttt agacaatcgg ccacttcggt tagaagagtt
    12661 tgaaaaacat gtcaatcaga tcatttatgt ctctgcgacg ccaggaccat atgaacatga
    12721 acaaacagat accgttatcc aacaaatcat ccgtccgaca gggctgcttg acccagtgat
    12781 cgaagttcgt ccaatcatgg gacaaatcga tgatctagta ggcgaaataa atgaacgagt
    12841 cgaaaaagac cagcgggtgt ttgtaacgac attgacgaaa aaaatggcag aagacctcac
    12901 agattatttc aaagaattag ggatcaaagt caaatatttg catagtgata tcaaaacgct
    12961 ggaacgaaca gaaatcattc gagatcttcg attaggtgaa tttgatgtgt tagttggaat
    13021 caatttgctt cgtgaaggat tagacgtacc tgaagtttcc cttgttgcta tcttggatgc
    13081 ggataaagaa ggctttttac gtagtgaacg ttccttagtc caaacgattg gacgagctgc
    13141 tcggaatgaa gaaggtaaag taatcatgta cgcagacaaa gtaacagatt ctatgcgtct
    13201 agcgatggat gaaacatcaa gacgccgtac gattcagcag aagtacaatg aagaacatgg
    13261 gatcgttcca aaaacgatca ttaaagaaat ccgagatttg atttctatta cgaaagaatc
    13321 ggaagatgat acaaaagaag cggtacaagt ctcgtatgaa gaaatgacga aagaagaaaa
    13381 agatacgtta ttgatgaaat tagaaaaaga aatgaaaaat gcggcgaaag cattggattt
    13441 tgaaacagcc gctaatgtgc gagatatgat tttagaatta aaagcttcaa agtaggagga
    13501 caacatggct aacgataaaa ttacgattca cggtgcacgt gcccataact tgaaaaatat
    13561 agatgtaacg attccacgag ataaattcgt ggtcgttaca ggtttgtccg gttctggaaa
    13621 aagttcccta gcatttgata cgctgtacgc tgaagggcag cggagatacg tagagagcct
    13681 ttctgcttat gctcgtcagt ttttaggaca aatggataag ccggacgtag atagcatcga
    13741 cggattgagt ccggcgattt ctattgatca gaaaacaaca agtaaaaatc ctcggtcgac
    13801 agtaggtact gtgacggaaa tcaatgacta tttacgcttg ttgtttgcac gtgtaggaca
    13861 tccgatttgt cccaacgatc atatcgagat taccagtcaa tcagtagatc aaatggcaga
    13921 tcaagtaatg gctttgcctg agcgcacaaa gatccaagtg ttggcaccga tcgtggtaaa
    13981 gaaaaaagga caacataaaa aaatattcga acgaatccaa aaagagggat acgtccgcgt
    14041 tcgagtcgat ggtgagatct atgatttaag tgaagtgcct gagctggaga aaaacaaaaa
    14101 acatgatatc gcgatcgtca tcgaccggat cattgtgaaa gaagggattc gttctcgtct
    14161 atttgattct ttagaggcgg ctctgcgatt ggcagacggc tatgctctgg ttgacgtgat
    14221 cggtgaagaa gaaatgttgt ttagcgaaca ctatgcctgt ccttattgcg gatttactgt
    14281 tggcgaacta gaaccaagac tgttctcatt caatgcgcct tttggagctt gtccagactg
    14341 tgatggttta ggtgtcaaat tagaagtcga tcaagattta gtgattccag atagaacgaa
    14401 gacacttagt caaggtgcta tcgttccatg gaatccaatc agctctcagt attatcctca
    14461 aatgttagcg caggcttgtg aaagtttcgg tatcgattta gatacaccat tcgaagaatt
    14521 accggaagag catcaggata tcatcttgaa tgggacagat gatcttttcc atttccatta
    14581 tgaaaatgat tttggtggcg ttcgcgatgt agaaacgact ttcgaagggg tattgaagaa
    14641 tatcgagcgt cgttatcatg atacgaacag tgatttcacg cgtgagcaaa tgcgtcttta
    14701 tatgacagaa ttaacttgtc agacatgtaa aggttatcga ttgaatcccc aagctctatc
    14761 tgttcaaatc aatggaacga atatcgggca aacaaatgag ttgtctattc aaaaagcagt
    14821 agatttcttc tcaaacttaa cgttatctga acaagagaaa gtaattgcca aaccgatttt
    14881 aaaagaagta aatgacagac tttcattctt ggaaaatgtg ggactagact atctaacttt
    14941 gagtcgcgct tccggaacat tatctggggg agaagcgcag cgaatcagat tggctacaca
    15001 aattggatcg aatctttctg gagttttgta tattttagat gaaccatcga tcggattgca
    15061 tcaacgcgac aacgatcgac tgattggctc attaaagaaa atgcgtgatc ttggcaacac
    15121 cctcgttgta gtcgaacatg atgaagatac gatgtgggca gcagattatc tgattgacgt
    15181 cggacctggg gccggagaac aaggcggaca aatcgtggct gcaggcactc ctgaagaagt
    15241 agctaatgat gaaaattctt tgacagggaa atatctttca gggaaaaaat caatccctgt
    15301 accaaaagaa cgccgtaaag gtaatgggaa agcaatcaag atcactggtg cttcagaaaa
    15361 caatctgaaa aatattagtg ttgaatttcc attaggagaa tttgtagcag tcacaggtgt
    15421 gtctggttct ggaaagagta cattggtcaa tagtatctta aagaaatcgt tagcgcaaaa
    15481 attaaataag aattctgcta agccaggtaa attcaagaca atttccggct acgaaagtat
    15541 cgaaaagatc atcgatatcg atcaaagccc aatcggccgg acgccgagaa gtaatccagc
    15601 gacttataca agtgtatttg atgatatccg tgggttattt gctcaaacga acgaggcaaa
    15661 aatgcggggt tataagaaag ggcgttttag tttcaacgta aaaggcggtc gttgtgaagc
    15721 ttgtcgcggg gatggaatta ttaagatcga aatgcacttt ttgcctgatg tctatgttcc
    15781 ttgtgaagta tgtcatggca aacgatataa ctctgaaaca ttagaagtgc attacaaagg
    15841 aaaaagcatt gctgatattt tggaaatgac agtagaagat gctgtagaat tcttcaagca
    15901 cattccaaag attcatcgca aactgcaaac gattgttgat gttggcttag gttatgtgac
    15961 gatggggcaa ccagcaacga cattgtccgg tggtgaggca caacggatga aacttgccag
    16021 tgaattgcac aaaatctcta atggaaagaa tttctatata ctagatgaac caacgacagg
    16081 acttcatagc gatgacatcg cccgcttgtt gcatgtatta caaagattag tagatgctgg
    16141 taacacagtt ttagtgattg aacacaatct agatgtaatc aaaacagcag attatatcat
    16201 tgatttagga ccagaaggtg gagaaggtgg aggaacgatc cttgcgactg gaacaccaga
    16261 agaaatcatt aacgtaaaag aaagttatac aggtcactat ttgaaaaaaa taatggtata
    16321 aagaggatag atagctgaga tagttaaaaa aacataaaaa aatgaatttt tgcttgtttt
    16381 attaatatca gtgttctaga atcattacat ggtagttctt tctacactga ttggtataga
    16441 caaaaacttc ctataagttc aatccgttca aaaaagaatt gagcaatcgg aggagtttcc
    16501 tttgattgct tttttcttta tggtatgata ttccttgtgt agttggaaaa gaaaaacaag
    16561 agattatgag taagtgagag ataatgatga acagtattga aaatgaagaa tttatcccta
    16621 tattattagg aagcgacatg aacgtttatg gtatggcacg ttcttttaat gaagcatacg
    16681 gcaaaatctg ccaagcatat gcttcggatc agttagcccc tacccgctac agtaaaattg
    16741 tcaatgtaga agttattcca ggatttgata aagaccctgt gtttatcgag acgatgcttc
    16801 gtttggcaaa agaaagatat tctgataaga gtaaaaaata tcttttgatt gcttgtggtg
    16861 atggatatgc agaattgatc tcccaacata aacaagagct gtcagaatat tttatctgtc
    16921 catatattga ttattcttta tttgaaagac tgatcaataa agtaagcttt tatgaagttt
    16981 gtgaagaata tgatcttccg tatcctaaaa cattgatcgt cagagaagaa atgttagtaa
    17041 atggacattt ggaacaggaa ttgccttttg aattcccagt tgctttgaaa cctgcaaaca
    17101 gtgtggagta tctttctgtc caatttgaag gaagaaaaaa agcttttatt ttagagacta
    17161 gagaagagtt cgatttaatt ttaggccgga tatacgaagc cggttataaa agtgaaatga
    17221 ttgtacaaga ttttatcccg ggtgatgata gtaatatgcg tgtgctaaat gcgtatgtag
    17281 atgaagatca tcaagttcgg atgatgtgtc ttggtcatcc actgttagaa gatcctacac
    17341 cagcatcgat tggtaattac gttgtgatca tgccagatta taacgaaaaa atctatcaaa
    17401 ccattaaggc atttttggaa aaaatcgaat atacaggctt tgccaatttc gatatgaagt
    17461 atgatcctag agatggagaa tataaattat ttgaaatcaa tttgagacaa ggtcgtagca
    17521 gtttctttgt gacattgaac ggcttgaatc tggctcgttt cgtcacagaa gatcgagtgt
    17581 ttaacaaacc ctttgttgaa acgacctatg gaacgaatca atcagataaa gcaagattat
    17641 ggatgggtgt gcccaaaaaa atctttttag aatatgctcg ggaaaatgaa gacaaaaaac
    17701 tagctgagca aatgatcaaa gaaaatcgtt acggtactac tgtgttttat gaaaaagacc
    17761 ggtcaatcaa gcgctggttg ttgatgaaat atatgttcca taactatatt ccaagattca
    17821 aaaagtattt tcatgtgaaa gaaggctagt tcatggagaa ttttttcagt attttaggcg
    17881 gaatgggcac gatggcgaca gaaagttttg ttcgcttgat caaccaccga acgaaagcta
    17941 caaaagatca agaatatttg aattatgtct tattcaatca tgcaacagtt cctgatcgta
    18001 cagcttatat tttagatcga tcagaagaaa atccaatgcc gtttctgctg gatgatattg
    18061 agaaacaaaa tttattgcag ccgaacttca ttgtgttgac ttgtaacacc gcacactact
    18121 tttttgaaga attacaagcg gcaacggata ttcctatcct tcatatgcca agagaagcag
    18181 caaatgaact tgtccgtcag catacgacag ggagagtagc gattctagga acagaaggaa
    18241 gtatgaaagc tggaatctat gaacgcgaag tcaaaaatct tggattcgaa acagtgattc
    18301 ctgatactgt tcttcaagaa aaaatcaatt atctgatcta tcatgagatc aaggaatcgg
    18361 attatttgaa tcaagagcta tattacgaaa tcttagaaga agcggtagag cgcctaaact
    18421 gtgaaaaagt tatcttaggt tgcacagaat tgtcattgat gcatgaattt gctgaagata
    18481 accattatcc agtgatcgat gcccaatcta ttttagcaga tcggacgatt gaacgagcgc
    18541 tagctgaaag aagcaaggca ttggatacag catcggaaaa gtaaaaaaat tggatttggg
    18601 gcaaatatcc taaaagatat ttggtccaaa tctttttgta aaagaaaaag aaaactggta
    18661 atgatacgcc cggaatgata taattttact atgcacaatg tgagaggagt tcgtaattat
    18721 gacagataat ttgcaattag tcatcataac aggtatgagc ggtgcaggga aaacagtagc
    18781 tatccaaagt tttgaagata tgggctattt ttgtatcgat aatatgccgc ctcgattgat
    18841 tccaaaattc tgggaattaa tcaaagaatc aggcaaagtc acaaaaatgg ctttaggagt
    18901 ggatttacgt tctcgttcct tctttgaaga aatccaaaac atgctgatcg aaatcgaaaa
    18961 tacgaacttc atcgacactt ctatcttatt tttagatgct tcagacgaag aactagtggc
    19021 tcgctacaaa gaaacaagac gagcacatcc aatggcgatg gacggtttga tcactgaagg
    19081 gatacgtaaa gaacgggcga tactagacga cttgaaagcc caagcttccg tgatcattga
    19141 tacgacgaca ttgacaccac gacaattacg tgaaaaaatc aatgagtcat tcaaatcaaa
    19201 gggagaccaa ggatttcgga tcgaatttgt atcatttggt tttaaatacg ggttgccgat
    19261 cgattcggat attgtgatgg atgtgcgttt tttgcccaac cctcattata tcccggaact
    19321 aagaccattg accggaaaag atcagccagt ctatgattat gtgatgtcct ttgatgaaac
    19381 gcaatcattc taccataaat ttttggatct tctggaaact gtattgcctg gatacattaa
    19441 agaaggaaaa agcagcttga cagttgcaat cggctgtaca ggcggtcaac accgttctgt
    19501 ggcattgact gaacgtttgg caaatgcttt agggaaaaac tataaagtca atgtgacaca
    19561 tcgggacaag gataaaagga aagagacggt gaaccggtcg tgaaaatgaa aacctaccgg
    19621 ataagaaaac caaagatcgt agtggtcggc ggtggcactg gtctgccagt tatcctgaaa
    19681 agtttgcgaa atcaaggagc agatatcaca gcagtagtga ctgttgcaga tgatggggga
    19741 agcagcggtg caatcagaga atcgatcgct atggctcctc ctggtgactt gagaaatgtc
    19801 ttagtggcgt tatcggatat gccacaattc tatgaagata ttttccaata ccgtttcaaa
    19861 aaggaagatc agtttttagc caatcatacg atcggtaatc tgatcattgc cgcagtttcc
    19921 gagatgcggg gaagcactta tgaggcgatt caattattag caaaaatgat gcatgtagat
    19981 ggacatgttt atccttcttc agaaactcct ttgaccttgc atgctgtctt taaagacgga
    20041 acgactgcag taggagaatc taaaatcgcg atcgaccgta aaaccattga ccgtgtgtat
    20101 gtctgcaaca caaatgataa aagtgaagcc aaagcggctc gaaaagtcgt ttcagcaatt
    20161 atggacgccg acatggtcgt tttgggaccc ggaagtttat tcaccagtat ccttccaaat
    20221 ttagtgatac cagaaatcgg cgaagcaatg ctcaagacaa ctgctgaaac cgtctatatc
    20281 tgtaatatca tgacccaaaa aggggaaacg gaacatttca cagatgccga tcatatacgt
    20341 gttttacaca agcatttgaa tcagcctttt atcgatacag tattggtcaa tacggaaaaa
    20401 gtgcctgaag attatatgga cccagagatc tatgatgagt atctagtaca agtcgcacac
    20461 gacttcaacg gattgagaaa tgaaggatgt cgagtgattt ccaccgactt tttggaattg
    20521 aaagatggtg gtgtcttcca cgatggagac aaagtagtag aagaattgtt ccgtttagtt
    20581 ttcggttcaa aatattaagc aatttattgt tggagaaagg aggagaatag gatgtcattt
    20641 gctgcagatg tgaagaaaga actaactggt ttggaagttc atcgagaaca tgcgaaagcg
    20701 gaattagctg ctttgctgcg aatgaacggc tctttaggta ttgtgaacca gcagtttatt
    20761 ttgaacgtcc aaacagaaaa tgctgcaatt gccagacgta tctattctct tttgaaagat
    20821 cattatcagg tacgaagtga gctactcgtt cgtaaaaaaa tgaaattaaa aaagaacaat
    20881 gtctatatcg tccggttgaa gcaaggcaca aaagatgtgt tgatggatct tgatatcatg
    20941 gatggtatga tgttcaatgg acacgtttcg gacgagatta tgggaaattc gcaaaaaatg
    21001 cgttcttact tacgtggtgc ttttatggcc tcaggttcag tcaataatcc ggaaacgagc
    21061 cgttatcatt tggaaatatt ttctatttat gaacagcata accaagacat ttgcgatatg
    21121 ttaaattatt acggtctaca tgctcgaacg cttgaacggc gcaatggcta tatttcttat
    21181 ttaaaaggag cagaaaaaat tgctgatttc ttaacattga ttggtgcaac caactcaatg
    21241 ctccgatttg aagatgttcg aatcgtacgg gatatgagaa attcggtcaa tcgtctagtc
    21301 aattgtgaga cagcaaatct gaacaagacg attgatgcgg cttctaaaca aatcgaaaac
    21361 atcgaattga tcgaagcaaa ggtcggtcta catgcccttc ctgaaaaact tcaggaaatc
    21421 gcagaacttc ggcttcaaca tccagaggtc agcttgaaag aattagggga aatgattccc
    21481 tcaggtgcga tatcaaaatc gggaattaat caccgaattc gcaaaataaa tgaatttgcg
    21541 gaaaaactgc gtgaagaagc agtttaatcc aaagaaaaaa ctgatacatg aagcaggaga
    21601 tgcgaatgta tcagtttttt ttaaaagtta tacttgcttt tcttccgtca aactataatt
    21661 ccatctttac tggagtaaca taatttggaa aggcttctat tgtttttaat acaatcgtat
    21721 aaaaagcggg gagacaactg aatgaagatt ttagttttgt acataaattt ttgcttaata
    21781 tagaagtcct gatatagata agatcaagta tttatctata actggcctga ttaattaaaa
    21841 aactggaagg aactgctcga tcatccttga gaactgctct tcttacttct ggtcaagatt
    21901 ctcgtggttg gtggataagg atggttgata agttcattgg attagctagt cgaaaacaac
    21961 caggtgtaat taatgtagaa gttttaacaa ggggatggag tgatttattc agtgataatc
    22021 ataagttcat aaatagtaat tgagatgtat ggttggaata cgttatacct gaacttgaac
    22081 taccaagatt tggtttagaa tttgagaatc gatgtttata aagggagggc aaaaatgcgt
    22141 ttttttacaa cttttactat cattatgatt gcagtacttt ttatttttct agatatagca
    22201 aagcgaaata cagcattttt attatatcgg gttttacttc gagcaggtct cataacattt
    22261 atatcgatcg ttggattttt tctattcaca gttattgtat ttatttggag aactccagca
    22321 ccaccattgc cagagattac atatggagaa tttcctttta gacttgagta tgaattgaat
    22381 gaagaattac atgtgataga agacacgtta atagttgaat ttgatggatt tggaatgaac
    22441 gaaggcatag ggagatatcg tagatggaca agtcgtttag ccagcggaga agatttagtg
    22501 ttgctgttag aagttagtga taataagcaa atattttatt ttccaggacc tgctaattat
    22561 tacatgggag ataggttgaa tggttataat catacttttc ctagtgcttc tttcattgaa
    22621 agggaaagag gcattactag aagggatatt ttacatgaca aagaattatt ag
//
LOCUS       DAXWLF010000028        22657 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXWLF010000028 DAXWLF010000000
VERSION     DAXWLF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.028"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(7..2388)
                     /locus_tag="ACGE4U_000843"
     CDS             complement(7..2388)
                     /locus_tag="ACGE4U_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFF8393029.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITERTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2378..3025)
                     /gene="recU"
                     /locus_tag="ACGE4U_000844"
     CDS             complement(2378..3025)
                     /gene="recU"
                     /locus_tag="ACGE4U_000844"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFF8393030.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3089..3628
                     /locus_tag="ACGE4U_000845"
     CDS             3089..3628
                     /locus_tag="ACGE4U_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFF8393031.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3718..4137
                     /gene="gpsB"
                     /locus_tag="ACGE4U_000846"
     CDS             3718..4137
                     /gene="gpsB"
                     /locus_tag="ACGE4U_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFF8393032.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4159..4526
                     /gene="rnpB"
                     /locus_tag="ACGE4U_000847"
     ncRNA           4159..4526
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACGE4U_000847"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4735..5901
                     /locus_tag="ACGE4U_000848"
     CDS             4735..5901
                     /locus_tag="ACGE4U_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFF8393033.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5947..7443
                     /locus_tag="ACGE4U_000849"
     CDS             5947..7443
                     /locus_tag="ACGE4U_000849"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFF8393034.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7524..8207
                     /locus_tag="ACGE4U_000850"
     CDS             7524..8207
                     /locus_tag="ACGE4U_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8393035.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8852..10042
                     /locus_tag="ACGE4U_000851"
     CDS             8852..10042
                     /locus_tag="ACGE4U_000851"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFF8393036.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10144..11097
                     /locus_tag="ACGE4U_000852"
     CDS             10144..11097
                     /locus_tag="ACGE4U_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFF8393037.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11166..12515
                     /locus_tag="ACGE4U_000853"
     CDS             11166..12515
                     /locus_tag="ACGE4U_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8393038.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12698..13021)
                     /locus_tag="ACGE4U_000854"
     CDS             complement(12698..13021)
                     /locus_tag="ACGE4U_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393039.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13150..14313
                     /locus_tag="ACGE4U_000855"
     CDS             13150..14313
                     /locus_tag="ACGE4U_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFF8393040.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASRQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14383..15732
                     /locus_tag="ACGE4U_000856"
     CDS             14383..15732
                     /locus_tag="ACGE4U_000856"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8393041.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15777..16208)
                     /locus_tag="ACGE4U_000857"
     CDS             complement(15777..16208)
                     /locus_tag="ACGE4U_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8393042.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16596..19238
                     /gene="alaS"
                     /locus_tag="ACGE4U_000858"
     CDS             16596..19238
                     /gene="alaS"
                     /locus_tag="ACGE4U_000858"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFF8393043.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19438..20034
                     /locus_tag="ACGE4U_000859"
     CDS             19438..20034
                     /locus_tag="ACGE4U_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8393044.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20156..21304
                     /locus_tag="ACGE4U_000860"
     CDS             20156..21304
                     /locus_tag="ACGE4U_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8393045.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21342..21755)
                     /locus_tag="ACGE4U_000861"
     CDS             complement(21342..21755)
                     /locus_tag="ACGE4U_000861"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFF8393046.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21834..22265
                     /locus_tag="ACGE4U_000862"
     CDS             21834..22265
                     /locus_tag="ACGE4U_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFF8393047.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22364..22597)
                     /locus_tag="ACGE4U_000863"
     CDS             complement(22364..22597)
                     /locus_tag="ACGE4U_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8393048.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
BASE COUNT         7278 a         4002 c         4751 g         6626 t
ORIGIN      
        1 tttttactat ctgtttccag aagctgaggt gctgctggat ggtgtatggt catttgcagc
       61 atccgctccg ctagttgctg gctcactgga agaagcagga ggagcagatg aagacggtgt
      121 cgagctttct tcggatgaag aagaaggaac cgttgttgaa gacgctggct cgcttgtttc
      181 tgctgtcgtg ctttcttttg aggactcagc agttgattcg ctttgactag tagagctaga
      241 gctttgagtc gttgtctctg ttgttgagct tccaggagtc tggacatagc tggaagatgg
      301 aatagttgtt gatggtgtaa ttgcattcga tctagctgta tattgatctt tgtagtacag
      361 ttcaccgccc acacgaatta atccgcttgg catctcccag tctgtatttg tcacgttagc
      421 tgaaacatac tgcataagct cgcgataaac atcagaggct acgtgtgacg attctgaagt
      481 gacaggtgtc atttttttat tgtaacctgt ccacacggaa atcgaataat ttggcgtata
      541 gcccgcaaat aggatatctg gatacacccc agaagaaata ccaagttttg catactcgtc
      601 atctgtgtaa ttggatgttc cggtcttacc ggcttgatat aacccagcga tttgcgcatt
      661 cgtaccagtt ctttcagtga tcgtatcttt taaaatgtct gtgatcatat aagctgtttc
      721 tggagacatg gctgtttttc catctggctc ataagtttct tctgttccgt cttggaagac
      781 gattttatta acatattgag gtttgtaata agtccctcca ttggcgaaag ccgcataggc
      841 tgctgccatt tttaaggaag aagctccgta ttttgtccca tcttgttctt ccgtattact
      901 agagatcgca ttcgattgat ggatcgtact gtattcgatc ccaagatttt ttaagaaaga
      961 agccacttta tctgaaccga cttcattgaa taatttgact gctggaacat tacgggataa
     1021 gtaaagtgct tggcgtaaag taatggtgcc catatattga ttatcccagt ttcctacagg
     1081 agtcgaagtc ccttcatagt tatacggtgc gtcagtaata gtttttccag tagaatattt
     1141 caaatattca aaagctggtc cgtaatctgt gacaggtttc atcgtagaac caaaatcacg
     1201 ggaagtattc actgctaagt tgttgcctaa agttacgtct tctgcgatgt gacgtccgcc
     1261 aatctgggct tttactttcc cggtattggt gtcgataagg gtggaggcaa cttgcatctc
     1321 gtcgtctggg taagagacat actgatccgt gttgacgata tcatatagtt tcttttgggc
     1381 gtccagatcc aaattcgtat aaatctctaa accgtctgtg tagacattct tatctgtttt
     1441 ttcttgtact tcattgatga cttctttgac ataattatcg acgatcttag tattgtcatc
     1501 ggattgagtt agttcttgca gaccatccgt taccggtaca ttaacagctt gatcatattc
     1561 tgtttgagag attttttcgt tttgaagcat cgtatacaaa acagtatctc gtcttttctt
     1621 cgcctgatct ggataaacat aaggatcata ggcggaaggg gcttgaggca tcccagcaag
     1681 caatgctgtt tgcggtaaag aaagttctga taatttctta ccaaagtaca tctcagaagc
     1741 tgtctccatc ccgtacaagc cgtttgacat atacacttta tttacgtagt aggtcaagat
     1801 ctcttgtttc gatttttttt gttccaatct tacagccatc cacgcttctt gggccttgcg
     1861 tttcagagtc tgatcctcgg cactggtgga gaagaaggaa agtttgatca attgctgtgt
     1921 caaagtactt ccgccttgca gtccgccaga ggtaaagtta gagagggcag aaccgatgat
     1981 ccgaataggg tcaaccccga tgtgtttata aaaacggcga tcttctaccg acacgatcgc
     2041 atcttctaat gttttcggta attcattcgc agaaattttt tcacgctttt ctgcgcctaa
     2101 atcttcgaat agttcgccat cttgtgtata tagtttggaa gatacggtag cgtctaattt
     2161 tttatccgtc aattcgggcg catcttttgc gtaataccag aaaagtccta cacctgctaa
     2221 aaagaggata cagaaaaatg agagtattcc taaaaggatc ttgataaata atcctttttt
     2281 atgtgttcca gaggactttc cagaaccttt gccagaattc ttcttcacac tctttttagg
     2341 agtaggctgt ttctgtcgtc tagagcttct agtttgttca tttgccattc ttgctttctc
     2401 cttcaataga taatgttgat aaatagaagt caaccgcgtc caaataagga atccgcggag
     2461 aaataccagt tttgatctct attcctagtt cttcgatcaa agataaaggt agtgatttgc
     2521 gtcctttttc cttttgggcg tcccagtaag caatcaaatg aacactattc aaataaaaac
     2581 agcgcttcaa agacgaaaac cataacaaga caaagcagac cccattttgt tcgaggcatg
     2641 ctttcatatg ttcgatttga tgggcatgga agtttttaaa gggaaaagat gttttgttct
     2701 tggtttcttt tgcttcaaag tccaagtaaa agcctcgata gacgccatta tagtctgtag
     2761 ttgaggcttg ggtaaagtag gcttccttga tcacagcagc acttctctta ggatagtcta
     2821 ctttcacgat ttggataggg gtaggctttt tatggataac cgccaacccg cgggaaagat
     2881 aatatttatt actttcattg attgcttctt caaagcgcat cccccgattg ccgaattcag
     2941 tgatctgatt tcttttttct tgaatttttt cgacgggaga ggcattctta taataaggtt
     3001 ggccatttgg atagcgcaat accatcttta atcacactcc taggccacat tatagcaaaa
     3061 aagcggatag aaagaaaagg aatctgagat ggataagtca aaagtaatct atgtgtctgg
     3121 gtatcgaagt ttcgaactcg gtgtttttaa agaaaatgat cctaaaatcc aagttgttaa
     3181 aaatgtttta aaaaaagaac tccatcagtt agcagaagaa gggctggaat gggtacttgt
     3241 cagtgggaat ttgggcgtcg aactatgggc tgtgcaaata gtagcggaat tgaaagaaga
     3301 atatccggaa ctatctcttg gtattttgta tcctttttct gattttggta ataactggaa
     3361 tgaagggaac caagaaaaaa aacaattagc agaacaatta gcagattatg tcgaagctgt
     3421 tagccatcaa ccctaccaat cacctagtca gctaaagaat cacactcgtt ttatacttga
     3481 gcatacagtg ggctgtctgc tgatctatga tgaagaattc cctggaaaaa cgaagtattt
     3541 ttttgaagat gcacagaatt accaagcaga tcatccctac gaaattcggc tgatcagtat
     3601 ggacgatctt caaaatttta gtgaataatc gatattgtgt ttgatttatc aacttttttc
     3661 ctatacaatg aaaaaggtag aaatgttatt acgtaaatga aaactagagg tgtaaatatg
     3721 gcgaatttgg tttatagtcc taaagacatt ttacaacaag aattcaaaac aaaaatgcgc
     3781 ggctatgatc ccgttgaagt tgatgaattc ttagacaata tcatcaaaga ttatgaaact
     3841 tacagcaaag aactccttgc tttacaagaa gaaaacgatc gattgagtgc aaaagtagct
     3901 caattatcta aaactcaagg agcagctcaa acacgcgtgc aacaaacaga ggcaccaaag
     3961 agcgcagcag tgaccaattt tgatatctta aaacgcttgt caaatcttga acgtgaagta
     4021 tttggtaaaa agcttgatca acaagcaagt gcagtcaaac cagcacaacc taatccaaat
     4081 aactacacaa atgcagacac aagcttagat gataatgaaa aaacacgtca attttaattt
     4141 ttgattaatg aatttgcggt tttcgggtaa tcgcggtctg acttgatcag gctgaggaaa
     4201 gtccatgctc gcacaggctg tgatgcctgt agtgttcgtg cttagcgaaa ccataagcta
     4261 aggtactctt tggagtaacg gcaggaaaac aagctaaggc ttcggctatg cttaagtatc
     4321 cttgaaagtg ccacagtgac gaagcggctg gggaaaccca tccggtggaa cgcggtaaac
     4381 ccctcgagcg agcaacccaa acaatggtag gggcgctttt tgtctggaag aagaacagga
     4441 caaaaaggca gtataactgc agatagatgg ttaccgcttt gttttttctc cctggaaaag
     4501 caaagtacag aacatggctt atagaaaacc gcaaacatca gggaattagg tcgtgacaaa
     4561 agtaatgcgt tataagaaac aagaaaaaca tttgaaagct atttttcaat acttcagatg
     4621 aatcgttctt attacaaaac aaacttttgt atcaccggtt attcggaatt tgagtaaaag
     4681 tgtggggtag gactgatgtc ccacctcctt tttttataaa taaggagatt gtgtatggaa
     4741 aaaaacagga agttcaacct cgtcgcaact gcggcaagcg ggctagaagc actggttggt
     4801 aaagaattaa gagatcttgg aatcgaatgc caagttgaga acggacgtgc acgttttgaa
     4861 ggaacaatgg aaacgatcgc tacagctaat ttatggttac gtacagcaga tcgagtaaaa
     4921 atcgtggttg gggaattcga tgcgtatacg tttgatgaat tgtttgaaaa cgtaaaagca
     4981 ttgccttggg aagattttct tccactagat gcagcttttc ctgtagctgg aaaatcgatc
     5041 aagtcgaaac tatatagcac acctgattgc caagcgatca caaaaaaagc aatcgttgaa
     5101 cgtctccgta cctattatca ccgtccagct tctgtaccac tgacggaaac tggcgcacat
     5161 tttcagttgg aagtagcttt gctgaaagac catgtgatgg taactctgga tacgacagga
     5221 ccaagtttat tcaaacgtgg ttatcgctta gaaaaaggtg gagcaccatt gaaagaaaac
     5281 atggcagcag cacttattat gttgacaaat tggcgaaaag accgtccatt ttatgatcct
     5341 gtatgtggtt caggtacgct ttgtattgaa gcagccttaa taggccataa tatcgcacca
     5401 ggattcaatc gtgagtttgc ttgcgaatct tgggattggt tcagtcaaga aatcatggaa
     5461 aatgttcgat cagcagccga agaaaaagca gattatgata ttgaattaga tattactggt
     5521 tcagatatca atggacggat gattgaaatt gccaaagcca atgcagaaga aatcggatta
     5581 ggctcttcga ttacctttaa gcaacaagca gtcaaagact tcaaaacgga taaagaatac
     5641 ggggtcatcg tggctaatcc gccttacgga gaacgtctag gagaagaaga aactgttcgg
     5701 aaattatata aagaaatggg cgatgtattc cgtccattaa aaacatggag taaatatatc
     5761 ttgactagcg acttagcgtt tgaggaattt tacggacaaa aagcaaccaa aaaaagaaaa
     5821 ttgtacaatg gtgcattgcg tacggatcta ttccagtatt ggggaactcg tccgcctaga
     5881 aaaaaagaga aagacgaata atcttactaa ttgattcatt caattggatg tttggaggaa
     5941 tagggaatga aagaaaaaga gtttttacaa gaagtaaaag aaatcaatct tctacaacaa
     6001 gctttaggaa tcctcgattg ggatacacaa acaggtatgc ctgaaaaagc tagtgcatac
     6061 cgaagtgaag tagacagcta tctttacagc atttattttt caaaaaaaat cggtccaaaa
     6121 atcaaagaag ccattcgcta tttttctgaa catccggaag aattgtcgga agttggaact
     6181 gccgtttttg ccaaagtaaa agaagagtat gatttggaac atcgcgtacc ggaagatctt
     6241 atgagaaaat atacagctgc cgtttcttca gcacacggac aatggcaaaa agctagagaa
     6301 acacaacaat tttctgattt tcaacacgca ttgaatcaaa atattgaatt aacaaaacag
     6361 ttgatacctt actggagaaa agatgaaaag acgaattatg atgttttgct gaatcaatac
     6421 gaacccggga tgactgtcga aatccttgat aaggtatttg atcagcttaa agaaggaatt
     6481 ctttcgatcc gccgtacgct ccaagaaaaa gggactgaac ctgaaacaga ctttttaaat
     6541 cggagaatga cgaaaaaaca acagaagaga tttgtagtga aagtaatcga acaacttggt
     6601 tatgatttta gtcgtggtcg tttagatgac actgtccacc catttatgac tggaattaat
     6661 ccgaatgatg cacgaatcac gacacgatgg aacgaaacag atttcaaaat ggctgttttt
     6721 ggagtgatcc acgaggctgg tcatggtatg tatgaacaag acatcgatga taaatatgcg
     6781 tatacaccgg tttatgaagg tacatcaatg ggtatccatg aatcacaatc gttgtttaac
     6841 gagatcatta ttggaagtag tcgcagcttc tggaaaaagc aatatccttt ctttcaagaa
     6901 tgtgcagaag gaacattcga tgatattcct tttgacaaat tttatcgttc attgaaccat
     6961 acaaaatcaa gtttggttcg tatcgaggca gacagtttga cttaccctct ccatatcatc
     7021 attcgttatg aaatcgaaaa aatgattttc aatgaagggg tagatgttgc tgaactacct
     7081 gaaatatgga atcaaaaata tgaagagtat ctgggaatac gaccaacaaa tgatgtggaa
     7141 ggcatcctac aagacgttca ttggtcaggt gcaagctttg gttattttcc ctcttatgca
     7201 ttagggtaca tgtatgcggc acaattactt cattctttgc agcaagaaat agatgtggaa
     7261 aatgttctag cgtcagacga ttattctcca atcaaaaact ggatgtcaga gcatatccat
     7321 caatatggtg catctagaaa accaaatcaa ttaatcatgg atgcaacaca tgaaccgctt
     7381 aatccgcaat acctgatcga ctatatgaaa gacctgtatt ttaatgtata tcaggtaaaa
     7441 taaaatacaa tttacaaaga tagctgaaat tcatctaatc tatagatgtt ttcagctatt
     7501 tacttaagaa agaagtggaa cgaatggata aattacaagt gatcgctgat tatagtatgg
     7561 accaattagc gcatgatcaa acaggacatg ggagcgatca taccaaaaga gttgtcaagt
     7621 tagctgaaaa aattttggag acggaaccgc aggcagaccg ctttgtgacg cttgcagcag
     7681 cttacctaca tgatacgatt gatgataaag tcgttaaaaa tgagaacgag gcaaagcaac
     7741 agcttcgtgt attccttcgc tctttgccaa tagtagagga acagatctcg atgatttttt
     7801 ccattatcga aaatatgtcc ttttctaaaa atctctctga agcggccgaa ctatctctgg
     7861 aaggtaagat cgttcaagac gctgatcgta tcgaagcatt aggagcaatc ggcattttgc
     7921 gtactgctta tttcggcggt ggacatgggc acccgatttt tgatcctgaa ctgtatccgc
     7981 aaacattcaa ggacaaagaa aattaccgaa aaggcacgac agtgatcaat catttttatg
     8041 aaaaactgtt cttgctgtct gataaaatga atactgatta cggatatgaa gaagccaaac
     8101 gaagagaaac cttcatgcgt gaattcttag aagaattttt tctggaatgg tctattgacg
     8161 atgaaaattt tactccagac aattggatca aaaataacaa aaaatgatgg aggttgtgac
     8221 agaagtggtc agctccaaga tatttcggat cattttccgt aaaagttact tctggaacac
     8281 tgtttaccaa gaggtcgtga gcctgccgtt cagctccgag tcacaaggaa caatttcaaa
     8341 aaatagcttc tcgtgtttga tgaatattgt aacttgtgat tcggaattgg caggagaaca
     8401 ccgtttattc ggaagaatag gactgtgaca agacttttgt cacagtcctt ttttcgaatt
     8461 caacaagtaa aacttttaag aaatattcag agagtaaaat taaaaattgc tgataaacga
     8521 agatggaaat tttatgtatt tttattggtc tatgcaataa ataaattaaa tattcagaat
     8581 acaattgatt ttaacgaaag ggcttctatc aatggttgtg ttgtatttat ctgcccaggt
     8641 agagtaaaat agatcattat ctaaaaggta attgttcata ctatataagt catttgaaag
     8701 taaaaagata ctgtaaataa aaaatgtggt taaataattc tcataatcag tgtaagcgtt
     8761 atcaaaaata tagcatactt gctttgtaga tgaatgacac gatatatgtt tggtatgttc
     8821 tgcaagacag atgaatatgg aggtggagac aatgtcttcc aaagaactaa caatgtattt
     8881 agagaaagaa ttggatcaac tgaaagaaaa ggggttatac aatacgatcg acatactaga
     8941 aagtgaaaat ggtgcttgta tcatcgtaga cggaaaaaag atgatcaatc tagcatccaa
     9001 caattatctt ggatttgcca atcgcgaaga attgaaaaaa gcatgtattg aagcaaccga
     9061 aacttatggt gtaggagcag gagcagtccg aacgatcaat ggttcgctga aaatccatca
     9121 gcaattagaa gaaaaaatcg cagaattcaa agggacagaa gcagcaatcg cctttcaatc
     9181 tggattcaac tgcaatatgg gcgctatctc tgcagtaatg acaaaagaag atgctatctt
     9241 atcggatgaa ttaaaccatg catctattat tgacggttgc cgcttatccg gagcaaaaat
     9301 catccgaatt aaacatcaag atatgaaaga ccttgagaaa aaagcaaagg aagcgataga
     9361 aagtaagaaa tataaaaaaa tcatgtatat cacagatggt gttttctcca tggatggaga
     9421 cattgcacga ttgccggaaa ttattccgat tgttgaaaaa tatggactga tcacctacgt
     9481 cgatgatgcg cacggctcag gcgtaactgg aaaaggtgca ggaaccgtca agcattttgg
     9541 attaagcgac aaaatcgata tgcaaatggg cacgttatcg aaagctatag gtgttgttgg
     9601 gggttatgta gctggatcga aaacactaat cgactggttg aaagctagaa gtcggccatt
     9661 cctattttct acttcgctaa ctccaggtgc agctgcagct gcattggctt cgattacttt
     9721 gatgcaagaa catccggaac ttgtcgaaaa agtttgggaa aatgctaatt attttaaaga
     9781 agaattgaaa aaagtaggct ataacatcgg tatgtctgaa acacctatta ctccagtcat
     9841 tttgggagac gaaaaagtga ctcagacttt ctctaagaag ctgatcgaac atgggattta
     9901 tgctaaacca atcgtttatc caactgtgcc tcttgggaca ggcaggatca gaaatatgcc
     9961 gactgcggaa catacgaggg aaatgcttga tgaagcagta gcagtttatc aaaaaatagg
    10021 aaaagaaatg gaaattattt aactaaattt agttttttcg tctatttaaa attagagaac
    10081 atgttcacgc atatattgtt aatcatctga agatagtagt aaaaaagatc agggaggatc
    10141 atcatgaaaa aagtattggt gacaggatgt ttaggacaaa ttggttcaga gttgatcaca
    10201 cggctacgtc aagaactagg cagtgaaaat gtcattgcga cagatatacg catgcctgaa
    10261 gtcattgaga aaaacgatat ctttgagatc ctagatgtaa tggattacga aaaaatgcgc
    10321 agcatagcag aacaatatca agtggacaca ttgattcatt tggctgctct tttgtcagct
    10381 gttgcagaag caaaaccaca attagcctgg aatttgaacg tgactgggct ggtcaatgca
    10441 ttggaagtcg ctagagaatt ccagctgaaa ttttttacgc caagttcaat cggcgcgttt
    10501 ggtccaaata ctccaaaaga tcatacccca caagatactc ttcagcgtcc tacaaccatg
    10561 tatggggtaa cgaaggtagc tggagaacta ctatgtgatt attattatgt gaaatatggc
    10621 gtagatactc gtggtgtccg tttccctgga ttgatttctt atgcaacctt gccaggaggc
    10681 ggtacgacag actacgcagt cgatatttat tattcagcaa tcaaagaagg aaagtacact
    10741 agtttcattg atcaaggtac atcaatggat atgatgtata tgccagatgc gatcgatgcc
    10801 attataaaac tcatgaatgc agatcctgat cgattggttc atcgaaatgc cttcaatata
    10861 tcggcgatgt cttttgaccc ggaagagatc aaagcatcta ttcaaaaaat cataccagat
    10921 tttgaaatgg attatgatgt ggatcctgta cgtcagtcaa tagcagaatc ttggccaaat
    10981 tcactagatg tttcttgtgc gcaagaggaa tggggctttt cgccgaaata cgacttagat
    11041 gtgatgacaa aggatatgtt ggaaaaactg aaagaaaaat tggtagagac ggtataaaac
    11101 aagaaccaca tccaaaaagt tttttatact tttgggattg gctatggtat gaaaggggac
    11161 aagggatgga agaatatcaa gccacgccag taaaagaagt aaaaatagaa aatgaattaa
    11221 aacgtacgat gggatttttt actgctttat cgactgttat gggcacagtg atcggtgcag
    11281 gggtattttt taaagccgca agcgtagcag aagttacagg atccgccagt ttgcatatgt
    11341 tttcttggtt tctcggtggt atgatttctg tctgtgcagg actaacagga gctgaactgg
    11401 cagctgctat tccagaaaca ggagggatga ttaaatatat cgaacggatt tacggaaata
    11461 cagcagcttt tttattgggc tgggcgcaag tcgtgattta ttttccagca aatgtagcag
    11521 ccttgtcgat cattttcggt acacagttcg tcaatctatt tggtctttcc cagtcaatga
    11581 ttgttcccgt agctgttaca gctgctgtat cgatcctgtt gatcaatttt ttaggttcta
    11641 aagctggagg ggcttttcag tcgattacgc ttgtctgcaa gcttattcct ttgtttgtga
    11701 tcgtcatttt cggtttgttc cgacaagaag gagtagactt ccaactgttt ccgatccaag
    11761 caggagaaaa tctttcattt ttctcagcct tgggagcggg cttgctagca acgatgttcg
    11821 catacgacgg ttggatacac gtaggaaata tttcgggaga attgaaaaag ccggcgaaag
    11881 atttgccaaa agcaatttct ttaggaatca tcggaatcat gatcgtttat ttattagtaa
    11941 atgcggtgtt cttaagaacc gcatctatcg acggagtcgc aggaaacagc aatgcggcaa
    12001 gtgacgttgc aaaaatgatt tttggcggct tcggtggaag actggtcaca gttggtatct
    12061 tgatttctgt ttatggaacc atcaatggct atacccttac tggaatgcgt ctcccttatg
    12121 ttatggcaaa agaaaacaac ctgcctttta gcaagctctt tgctaaactt catgataaaa
    12181 ctaaagttcc tgtagccgca ggtatcttag agctagtcat cgctattggc atgatgatga
    12241 tcggtggttt tgatacacta acggatatgc tgatctttgt catttggatt ttttatacga
    12301 tggtctttgt tggtgtcatt cttcttcgta aaaaagaacc tgatttgttt cgaccataca
    12361 aagttccaat gtatccgttt attccactag tagcaatcat tggagggact tttatcgtat
    12421 caagcacgct tatcactcag acctttttag cttcgatggg gatagtattg acacttgctg
    12481 gtatcccgat ttatctctat ttaaaacgta gatagctaaa acattcgaat tttattagaa
    12541 ctgcacttct ctgccaaaca aaatatttta tttaaatgag gagtacgtgg aagaaatgtc
    12601 aaaagtactc gttaattttg atcacgtaaa tgtcgttttg tctacgatga aatagcaaag
    12661 taaaagacag aaatcgggct caaccgattt ctgtctttta ctttgatttg cgtaactgaa
    12721 ctattcggcg tacgccgcga taaatattta ataaaaccaa gcaaccagaa atgaaaatgg
    12781 gaagccaaat cagatagaca gggtagccaa gcactttaga caaagcatgt ggagtcattt
    12841 tatccatttc aagaagtgct tgctgccaag tctgaagatt gaagactagc atgaaacccg
    12901 aaataatcag gagaataaat cctaaataat gaattgtagt tctttttctt acataagcga
    12961 tcgtagcata aaggaacagt aaaaaaatag gtacgccaaa aacaaagtac atattcagca
    13021 tctcgacacc ccctttgtgt ttatcttact ttactttgaa agaaatgaga aataatagcc
    13081 tcgttacttg cttgaaacag caaaggactg ctaagataag agagatacaa agaatcaagg
    13141 aggagtcata tggcaagtta tatcgaaccg attcgttttg cgctgattgt ttttccattt
    13201 ttagccttag cgatctcatc tgtttttttt atttatgaat atcgaaagta tggaacattt
    13261 ctagtaacaa gggcagttat tttgtattct ttcgtttttt atttattgtg tgcgtatttt
    13321 ttggtgatct taccacttcc ggcaaaggag acggttgccc aaatgacagg gccaaagatg
    13381 gagctgcatt tatttgcttc aaggcaacat ttcagagaca acacggtatt ggtcttgtct
    13441 gaacctagta cctacctgcc ggcaatgaag cagagtgtat ttttggaacc agcttttaat
    13501 gttttattac tcataccatt tggcatttat ttaagatatt atttccggct ttcttttatg
    13561 aaaacgatga ttgctagttt ctgcctttct ctattttttg aattgacgca gctaagtgga
    13621 ctttatttta tctacccaag accataccgc ttatttgatg tgaatgattt agtgacaaat
    13681 acattgggtg gaatcattgg ttttgtcgtt acgccgttat ttacatttat gcttcctaca
    13741 agaagacgaa tggatgaact ttcttatgaa aaaggaaaga ctgtctcatt gactcgacgc
    13801 ttggtcgctt atttgatcga ctggacattt ttatctctta ttaatttggt agcggcgatt
    13861 ttctttaaaa ttgcgttcga ttcaggggca gttacttctc atatctggtg gcttttatta
    13921 gaagtagggg tatactttat gattgtaccg tttataacca atggatggac acttggcaaa
    13981 aaaattgtgc gtatccgttt ggtggaagaa gaccgcgaac gaatttcttt aaaagcattg
    14041 atgatccgct acgggtatct gtactttctt ttttatggtt taagctggtt ggggcagttg
    14101 cttcagcaaa tcatgaattc gacaacgaat acggtgttgc agatgagtgc agtattcatt
    14161 tctctcggtg tcttgttact acaaggtatt tttattttaa atatcttgct atcatttttg
    14221 agaaaaaaac ggattctgtt ttatgaaaaa gctagtcaca catatactgt tagcacgatt
    14281 ggccaaacaa aggatgatga ttctcgaaat tagctgagca aaaacgcttt gtctgtgtta
    14341 taatgccaaa gtcgtgataa tttaaagaga agaggtgcac gaatgaataa ttttgatata
    14401 ttccgcttca aaccgtttat caatgaagcg ttaaccgcaa agggcttcaa acagcccaca
    14461 gaggtccagg aacgtttgat tcctttaatt gcaaaaggaa aaaatatcat tgggcaatct
    14521 caaactggta caggaaaaac acatacgttt ttgttgcctt tgtttgaaaa aatcaatgca
    14581 gaaaaagagg aagtacaagt tgttattaca gctccaagta gggaactagc tgcacagatc
    14641 tatgaagcag ctacacaaat cgctaaattt tctacaccgg agatccgtgt ctcgaatttt
    14701 gtcggcggta cagacaaaca gcgtcaatta aatcgtctgc atcaccaaca gccacaggtt
    14761 gtagtaggca cgcctggtag aatattagac atgatgaatg aacaagcgct gaagattcac
    14821 accgcatttg catttgttgt cgatgaagcg gatatgacat tggatatggg ttttttaaat
    14881 gaagtagacc aaattgcaag tcgattacca gaaaagctgc agtttttagt tttttctgcc
    14941 actattccag aaaaacttcg tccattctta cgaaaataca tggaaaatcc aatcattgaa
    15001 gaaatcaaac caaaagcagt tatttctgaa gacattgaaa attggctgat ttcaacaaaa
    15061 ggtaaagatg agaatcaaat gatttatcaa ctgctgacaa ttggtcatcc ttatttagca
    15121 atcgtatttg ccaatacaaa acagcatgtg gatgaaattg ccgattactt gaaaaaccaa
    15181 ggcttgaaag tagcaaagat tcatggagac atcaccccaa gagagagaaa acgagtgatg
    15241 cgacaagtac agaatctgga gtatcaatat gttgtagcga ctgatttagc tgctagaggg
    15301 atcgatatcg aaggtgtctc tcatgttatt aatgcagaaa ttccagaaga tctagacttc
    15361 tttatccatc gtgtcggacg tactggacgt aatggcttaa aaggaacggc cattacgtta
    15421 tattcgccaa atgatgagca agccattgat gcgatagaaa aattgggcgt ctcatttgaa
    15481 ccaaaagaaa tcaaaaacgg ggaaatcgtc aaaacatacg atcgtaatag acgtgccaaa
    15541 cgagaaaaat caaaagatgt attagatccc acgctgattg gtcttgtgaa gaagaaaaag
    15601 aaaaagatca agcctggtta taagaaaaaa atcgattggg caattgcaga aaaaaataaa
    15661 aaagaacgca aaatcgaacg cagacagcaa agccgtactg ctcgtaaaaa taagaaaaat
    15721 tcacataaat aaaaaacaag aggttgtgac aaaaggcgtg tcacaatctc ttgtttttaa
    15781 gctagacttt ttttcattcc gatacaaagt acttcatctt caataaaagg ttctgtgcaa
    15841 gtatgatacc cttgtttttc ataaaaatct acagccgtca tttcagctac tacataacta
    15901 gtctgacaac cttctgaaac cgcccgtttt tcagcagctt caagcaactg accgcctaac
    15961 ccttttcctc ggtattcttt tgctacgcaa aatcgttccg gcttgaagca agtaggtgtt
    16021 ttcttttcat aacgcaaagt agctactggt cttttttgat caagaaatag ataataggtt
    16081 tgttgcggga tatcttttcc gtcgaattct agttctgcag gaatcccttg ttctaataca
    16141 aaaacttgat atctcaaaaa gcagctagct gcgcgtatcc attcatccgc tccaactatt
    16201 tttttcatca tattcctcct tgtcatgaat agcagagatg tattgattcc tttattctta
    16261 tcgtaccaaa aatcgtggag tatgaccaaa aggaaatgaa aataaaaatt gagatattga
    16321 cagtgatcaa tagaatgcgt atactaatta agtacgaaaa ggatatgatt tgttttttaa
    16381 gactcacaga aaattctttc ttggctgaaa aaagaatagt cactaatcaa attgctccct
    16441 ttttctattt tggtagaaat accgacgcag ctcagcgtta tcgagtttaa gaggtaatga
    16501 ttacacatca ttgcaatcaa ggtggtaccg cgagttttcg tccttggctg tgatggtgtt
    16561 tttttgtatt taatttatag aaagcaggta atgacatgaa acaattaagt agtgcacaag
    16621 ttcgacaaat gtttcttgat ttcttccaat ctaaaggaca tacgattgaa cctagtgcgt
    16681 ctttgatccc cgtgaacgat ccaaccttat tgtggatcaa ctctggtgtt gcaacattga
    16741 aaaaatattt tgatggttca gttgtgccag aaaatccaag aatcacaaat gcccaaaaat
    16801 ctattcgaac aaatgatatc gaaaatgtcg gtaaaacagc acggcaccat acgatgtttg
    16861 agatgctggg taatttttcg attggtgact atttcaaaaa tgaagccatc cactgggcat
    16921 gggaattttt gactgatcca aaatggatgg cattcgatcc agaaaaactt tatgtaacag
    16981 tttatccaaa agatacagaa gcaaaacgca tctggcatga agaagttggc ctgccactag
    17041 atcacattat tgatgtagaa gacaacttct gggatatcgg tgcaggccca tgtggtccgg
    17101 atacggaaat tttctatgac cgtggagaag cttataacga tttagcagaa gacgatcctg
    17161 aaaactatcc aggaggagaa aatgaacgtt atttagaaat ctggaactta gtattttcag
    17221 aattcaacca taagcctgat aacacatttg aaccacttcc tcataaaaac attgatacgg
    17281 ggatgggatt ggaacgcatg gtctcaatcg ttcaagatgc gtcaacgaac tttgagacag
    17341 acttgtttat gcctattatc cacgccgtag aaaagctaag caaacaagta aaatatggtg
    17401 aaaacccagt aacagatatt tcttacaaag taatcgcaga tcatattcgc gccctttcat
    17461 ttgctattgg cgacggtgca ttgccatcga atgaagggag agggtatgtc ttacgccgtt
    17521 tgcttcgtcg tgctgtcatg catgggaaaa aattaggtat tgaacaagca ttcttatatc
    17581 gtttagtccc tgttgttggg gaaatcatgg taagttatta tcctgaagta cttcaacaaa
    17641 aagaatttat tgaaaaagtt atccgtacag aagaagaacg tttcaatgaa acaatcaatg
    17701 aaggtttgga aatcttaaat caagtgatcc aagaagtaaa aaacgaaaac gggacgactc
    17761 tagcaggaaa agacatcttc aaactatacg acacatatgg cttcccggta gaattaacgg
    17821 aagaggtggc agaagatgaa ggactgaaag ttgatcatgc tggttttgaa aaagaaatgg
    17881 atgcacaacg cgaacgtgct cgctctgccc gcagtactga aaaatcaatg ggggtacaat
    17941 ctgctttatt gacagatatc aaagtagaaa gtaaattcat tggatacaca gaacaagaat
    18001 cagatagtca attattggtc atcgttaaag gtgagtctct tttgaatgaa gtcgcagaag
    18061 gtgaagctca aatgatcttt gatcaaacac ctttctatgc agaaatgggt gggcaagtag
    18121 cggatcaagg atggatcatg aatgaagtag gcgaaatcgt agcagttgtt acagatgttc
    18181 aaaaagcgcc taatggtcaa tttatgcaca ccgtagaagt aaaagcacca atgagtgaag
    18241 gacaaaccta tcatctattg atcgatcaat cgatgcgcaa tcggatcatc aaaaaccata
    18301 cagcaaccca tctactgcac aaggcattga aagaagtact tggcgagcat gcaaatcaag
    18361 ctggttcttt agtcgctcct ggacatttac gattcgactt tacccacttt ggtcaaatca
    18421 cttcagatga attagctgaa atggaaagaa tcgtcaacga aaaaatctgg gaagctatcc
    18481 cagttgaaac aattgaaaca gatatcgata cagccaaaaa catgggtgca atggctttgt
    18541 ttggtgaaaa atacggacat gacgttcgag tagtaaatat tggcggctgg tcaatcgaac
    18601 tgtgcggagg cacacatgta gcaaatacag aaaacatcgg aatcttcaaa atcgtttctg
    18661 aatcaggaat tggtgcaggc gtgcgacgaa tcgaggctgt aacaagtaaa gaagcgtacg
    18721 aactacttga agaagaagaa aaacaactaa aacaagtagc aacaattgtt aaatcacctc
    18781 aattgaaaga agttgtttca aaagcggaac aattgcaaca gcaagtaaga gagctacaaa
    18841 aggaaaatga acaattagct agcaagttag cgaatcaaca agctggtgat gtattcaaag
    18901 atccagaaac gatcaatgga aaaacaatca ttgcagcaca agtaaaagtg aaagatatga
    18961 atcagctgcg tcaattagct gaccaatgga aacaaaaaaa tatttcagat attcttgttt
    19021 tagcaaacga acaagaagga aaagtcagtc tgctagcggc aatgacgaaa gaatcaaatg
    19081 aagctggact aaaagctggc gacttgatta aagcaatcgc accgaaagta ggcggtggcg
    19141 gtggtggacg tccagacatg gcacaagctg gcgggaaaaa tcctgctggt attccagatg
    19201 cacttaaagc tgcgaaagaa tgggttgcgc aacaataggg cgtttacgtt tgacagaaaa
    19261 acaaaagttc aaacattgaa cgaaacatga ttatgggggc gaagacaaac aagtcttgcc
    19321 ctcatacttt tttcaattgg atggcgcatc taatcaaatg gtcggattcg gatggtcttt
    19381 agttgacgaa agtgacagaa atttttacga ttagtataaa taaaaagggg tgaaaaaatg
    19441 aatagtagaa ataagacctt tcgactagtc ttacgtgcga tcttgttagc aatcatcatc
    19501 gtacaagcga tggttccttg gctggggttt attccattag gattcattag tttaacaatt
    19561 atccatatca cagtaattat tgctgcagta gtattaggcc caaaagatgg catggtgatt
    19621 ggcttgtttt ggggaatcgc tacaattgtt cgtgcctatg ccatgccaac aacgccattt
    19681 gatacattag tgttcacgaa tccgattatc tcggtagttc ccagagtact tgttggctta
    19741 gtagcaggct tggttttcca ctggatttat caaaaaaata aatcaataac gatcagctca
    19801 gcctttgcag gggtactggg ttcattggtc aatacagtct tagtgttagg attcatggga
    19861 ttattttata caggtgcaac ggcagatgcc tatggtgtag actctgctct tttatttaaa
    19921 accttagcag gggttgcagc aatcaatggg ataccagaag caataggtgc gggaatcata
    19981 acaccattac tagcaaaagc gttatttgca gcaacaccgt tgaaaccaga ataatgacca
    20041 tcttttaata gtaataggac gcacgataga tagaaaaagg aagagaggag aggaaaacaa
    20101 tcgtcctctc catcttttta cctgagcaac aagcaaacta gaaggggtaa aaaacttggc
    20161 taagaaacaa gaagaaaaaa agaaacaaat accgcaacag cggacaaaaa aaggagtact
    20221 agcgggtatc ttaggattgg ttacgaatct gatcttgttt gtctctaagc taatgatcgg
    20281 gcttttttca ggaagtgtct ccattatggc tgatgcagtg aacagtctat cagatactgc
    20341 ttcatccata ttgactttga tcggatttaa aattgctgca aagccagcag atcaggaaca
    20401 tccttatgga cacgaacgct tcgaatatat cagtgggtta tttgtttcga tcatcattac
    20461 ttatgtagga tttcaatttt tagagtcttc tgctgaaaaa atcatccatc cagaaaatgt
    20521 ctctttaaca cccatcgtct tttttgtcct aatcatttcc atctttttga aatttgcaca
    20581 aggcaaaatg tatcaggtta ttgcgcaatc cattgattct gaaacattga gagctacagc
    20641 aacagatagt tataatgatg tctttactac actggctgta ttgctttcag cagcaatcga
    20701 atgggtaact ggatggcgta ttgatggata tataggtttt gctttagcat tatatatcct
    20761 ttatagtgga ttgacgatgc ttcgaaattt tgtatacgaa ctattaggaa gtcgcccaac
    20821 gccagcagag attgaagaaa tggaagagca tttaaatgct tgcgatgata tacttggcta
    20881 tcatgattta ttggtgcata actatggacc caataaacgc tttgcatcag tccacatcga
    20941 agtagatgaa tcaatgaatt tgaatgtggc tcataagatt attgatctga tagaaaaaga
    21001 ctttaaaaag tctttggatg tggaacttgt ctgtcacttg gatccaatgc ctgttgataa
    21061 cgaaaaatac tataatatct tgcggcagtt aaagcaaatt cttcttgaaa caggtacagg
    21121 gcttaatatt catgatttta gagtaattca tcaaggaaaa acgctgcaat ttgatgtagt
    21181 ggtccctgag aaattccagt ttgttgatac agaactagaa agaatcttac gtagtcaaat
    21241 agaagaaaaa atcggagatt accaattgga aattacgttt gatcacaatt atcttttata
    21301 ttaaatgaga aactataaaa agactcctta aagtttttaa tttaaagagt ctttttttct
    21361 tttaattgtt tatggagagc ctgacgtgca agattatctg ctcctttatt tttgttttcg
    21421 ggcaaccatt gcaggatcaa taaagaaaat tcggatagaa gttcttggat tctttgaagg
    21481 tagggaagga agagctcgtt cttcgttgcg tttttgtcga ccgtagaaac tagtgttttg
    21541 ctgtctgaaa aacagaaaat cgtttcattc tgtaagtcgt ttttctttag aaattctaaa
    21601 gtttttaaaa agacttcgaa ctctgcttga tggtttgtcc ctatagaaag cgggatagat
    21661 agctgttctt gtttaccttg atagacaatc aagattccac ctccactagg accagggttg
    21721 ccttttgtag aggcatcaat gtatactttt aacataaaaa gagattctcc tttttgttca
    21781 aactaatgtt atggtaagat tatatcatta ctgaatcggt gagaggttag agaatgaaaa
    21841 aaaatattcg atggcagcct gaactggcac atacgatcat ctattggtca ttgaccttga
    21901 tcattctgtt ttttagtctg attctttctt tagaaaacac aggtccttat tggaaaagca
    21961 attttgtttt aggtattttt cttgtttttc tatttctagg tttgcggcgt tcttttattt
    22021 taaaatcaaa ctctgtgatt atacgttatg ctgctttttg gaaagaccga gaaatatctg
    22081 tcggacagat tcgatctttg acgccaacta atactggggt gcaaatgatg ttgcaagatg
    22141 agaaagcccc atctgttttc atgatgcgaa aaaaagatca agtagtcctt tttgatcatt
    22201 ttgaaaaaaa cggacccaaa tcagaatcaa ttccttatcc ttatacgaaa aaaagaaaaa
    22261 gctaatttta aaaatcagaa cctaaaaaaa cgaattttta caggaaaaga aaagagtgag
    22321 acgtacggtt catcccgttt agcgtctcac tctatttgtt tatctattca tgcatactag
    22381 tatcggtagt ctgaacgttg cctttcttga catgggcagc ttgtgttcct cgattccctt
    22441 cgatgatctc aaattcgacc acttgatttt cttccaaagt tttgaatccc tcttcttcaa
    22501 tcgcagtaaa atgtacaaaa atttcttctg tgtcatcata actgataaag ccgtatcctt
    22561 ttttgttgtc aaaccatttt acaattcctg tggtcaaagc actcacgacc tttctaaagt
    22621 ctgatcatag ccttcattat aaagagtatt tataagt
//
LOCUS       DAXWLF010000029        21973 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXWLF010000029 DAXWLF010000000
VERSION     DAXWLF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.029"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(235..348)
                     /locus_tag="ACGE4U_000864"
                     /pseudo
     CDS             complement(235..348)
                     /locus_tag="ACGE4U_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(731..1963)
                     /locus_tag="ACGE4U_000865"
     CDS             complement(731..1963)
                     /locus_tag="ACGE4U_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFF8393049.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2217..2786
                     /locus_tag="ACGE4U_000866"
     CDS             2217..2786
                     /locus_tag="ACGE4U_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8393050.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2964..3404)
                     /locus_tag="ACGE4U_000867"
     CDS             complement(2964..3404)
                     /locus_tag="ACGE4U_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFF8393051.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3562..4326
                     /gene="map"
                     /locus_tag="ACGE4U_000868"
     CDS             3562..4326
                     /gene="map"
                     /locus_tag="ACGE4U_000868"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFF8393052.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4358..5281
                     /locus_tag="ACGE4U_000869"
     CDS             4358..5281
                     /locus_tag="ACGE4U_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFF8393053.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIVPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5357..6496
                     /locus_tag="ACGE4U_000870"
     CDS             5357..6496
                     /locus_tag="ACGE4U_000870"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFF8393054.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6489..7289
                     /locus_tag="ACGE4U_000871"
     CDS             6489..7289
                     /locus_tag="ACGE4U_000871"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8393055.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7289..8116
                     /locus_tag="ACGE4U_000872"
     CDS             7289..8116
                     /locus_tag="ACGE4U_000872"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8393056.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8121..8828
                     /locus_tag="ACGE4U_000873"
     CDS             8121..8828
                     /locus_tag="ACGE4U_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8393057.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8928..9794
                     /gene="rfbA"
                     /locus_tag="ACGE4U_000874"
     CDS             8928..9794
                     /gene="rfbA"
                     /locus_tag="ACGE4U_000874"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFF8393058.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9808..10380
                     /gene="rfbC"
                     /locus_tag="ACGE4U_000875"
     CDS             9808..10380
                     /gene="rfbC"
                     /locus_tag="ACGE4U_000875"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFF8393059.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10402..11430
                     /gene="rfbB"
                     /locus_tag="ACGE4U_000876"
     CDS             10402..11430
                     /gene="rfbB"
                     /locus_tag="ACGE4U_000876"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFF8393060.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11528..12379
                     /gene="rfbD"
                     /locus_tag="ACGE4U_000877"
     CDS             11528..12379
                     /gene="rfbD"
                     /locus_tag="ACGE4U_000877"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFF8393061.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12414..14447
                     /locus_tag="ACGE4U_000878"
     CDS             12414..14447
                     /locus_tag="ACGE4U_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393062.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKQPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14491..15771
                     /locus_tag="ACGE4U_000879"
     CDS             14491..15771
                     /locus_tag="ACGE4U_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFF8393063.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15981..16787
                     /locus_tag="ACGE4U_000880"
     CDS             15981..16787
                     /locus_tag="ACGE4U_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8393064.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16799..18019
                     /locus_tag="ACGE4U_000881"
     CDS             16799..18019
                     /locus_tag="ACGE4U_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393065.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18009..19595
                     /locus_tag="ACGE4U_000882"
     CDS             18009..19595
                     /locus_tag="ACGE4U_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8393066.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19634..21772
                     /locus_tag="ACGE4U_000883"
     CDS             19634..21772
                     /locus_tag="ACGE4U_000883"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8393067.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21840..>21973)
                     /locus_tag="ACGE4U_000884"
     CDS             complement(21840..>21973)
                     /locus_tag="ACGE4U_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8393068.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         6929 a         3715 c         4562 g         6767 t
ORIGIN      
        1 tacgaacgaa tgttcttttt gtgaaggcgg aatttaatat actgaataat agaaaacaag
       61 tacttgtgcc aaattatgtg ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa
      121 aaccttctat attaagattt cctgcaataa caaacacaac agaagacgtg taatagttag
      181 tcaggaacgt acaaataacc cctgtatcct ttgcggtaca gggggttatt ttgtataagt
      241 agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac
      301 tccttgataa agtaaaatag atgcatgcgt atgtcttagt ccatcaataa acagtcgaag
      361 agaaaatagg gaaaaattga ttactaaaga aaaacttact aggtcgacgg tgtagttgat
      421 tttcatactt caatagattc gaattaaacg atttcgattg gcttatacca gtcctatcca
      481 acgaaagata tatcgttctt aactctagcg gtataacttg aagctttacg taaagccaat
      541 gggaatgaac tggagatgac tttttattat ttttagtaat agctgatggg ttaatactta
      601 caactttcct tttcaaagta ccatataaaa aaacggtggg gttgtttagc gaaataaagg
      661 atctaagcca atagcttaga tcctttattc tatgctttat attatttttt gcatgtgtgc
      721 gaaagtatac tcaagcccaa tcgccatttc ggaaaatcgg tactgtagag ccatcttcac
      781 ggattccgtc gatatccatc ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc
      841 tattcaatcc agcttctgct aattcttcat cggacatttc tgtaccgcct ttcacactga
      901 aggcataagc agaacctagt gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga
      961 aaaagatgat gccagattga gaaataggtg aagggtcagg tacaagtgcg acttctccta
     1021 aacgacgcgc accttcatct gtatcaagca gttttgctaa gacatcttct ccttgttcgg
     1081 cagaaaaatc aacaactttt ccatctttaa aagtaaattt catacccgag atgatggtgc
     1141 cagcataact cagtggcttt gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg
     1201 ctgtgaacac ttcttccgtc ggcatatttg ccatgaattt ttctccacga gcattatagc
     1261 ttccagcgcc ttcccaaaga tggtttttag gcaatccgat aatgatatct gtaccgggag
     1321 cagtatagtg aagggcagaa aattgttctc gattcaattc ttctgctttt tttgctaatt
     1381 tttcatcatg cttcttccat gctaagacag ggtcttcttc atagacacga gtcgttttga
     1441 aaatctgatc ccataatgcc tcaacttgtt tttcttctgg cagttctggg aagacttttg
     1501 ctgcccattg tttaccagcc gcagccacga cagtccaact gactttattt gcttgagttg
     1561 ccttgcgtaa attcattagt gctttgccat tcgctgattg ataagacgct actcgatggc
     1621 tatctacacc agcaaatgca tcgggatcgg cagaaacaac gctgatcctg cttgctcctt
     1681 tttcaagcca ttcatccgct tgatcgattt tagattgtgg tacattctca atacgatccg
     1741 ttgcagcatg taaaagaaat tctctttgga tctggtcgtc agtccattga acgataacct
     1801 cagcggctcc tagtttataa gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca
     1861 cgctgatctg taaaacaact gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta
     1921 agcgtgcata tttttttaga agttcattaa aatcagataa cataataaaa attcctcctc
     1981 accatttttt ctggtaacta ttagcatgat accatttaat tatgattcaa acaatgaggc
     2041 tttaaataaa aaaaggaggt tattttttta aggttaagtt tcatgatata tgttactact
     2101 tgctatttca gcttgtgaag aaaatcaaaa actaattttg catatcaaaa aaacatgtta
     2161 caatgaaatt aatcaatggt ggaagcgtta taaaaaaatt aaggagatta aaaaatatgg
     2221 caagaaaaaa gacgattact agagaacaga ttttggcagc tgcttatgaa gtagctgcca
     2281 ccgatggatt tacacgattt actgcgagaa acatcgcgaa caaaatgaat tgttccacgc
     2341 aaccgattta tcttgagttt aaaaatatgg atgacttgag acaagctctt tttgagcaga
     2401 tctatgatca tttacaatat gaagtgttca ctgttgagca tacagggaat acgattatcg
     2461 atcttgcttt taattatatc cattttgcaa gaagagagaa gaagttgtat cgttcactgt
     2521 acttagaaga atatggtgga ggaaaagaga tgcagaattt ctcttatact tactttgtga
     2581 atacagtgaa acgcgatcca gcttatgtga atctttcaga agagcagatc gaatctttgt
     2641 ataatgggac atggatcata gctactggga tcgcagcttt gatgtcttca agcatcatcc
     2701 atccaactga tgaacagatt gaaaagatga tccaagacag tatagatgct atcttgaaat
     2761 tcaaagaacc aatcgaaatc gattgaatat actcaaaagt tccacaaatc agtaggtaac
     2821 aagagatgtg tcaaaataag gaaaagttcg tttttcttac agcgtatgca tttttaactc
     2881 ccgaagaact cttccatgaa tgcaatgaat aaaagagcca tacagattga ataaatctgg
     2941 atggctcttt tattgcaagt atattaaatt tttgcgacta gttctttagc gaaagcttct
     3001 aaattttcga tatcttcttc ttcagcattc agatccactt taacattttc ggctcctttt
     3061 gatgcgcctg tttttgcaaa agcagcatcg aaatcatcca ccgatttgca gaattcatcg
     3121 taaaaagtat caccagatcc gcagacaccg taaatttttc ccaatagatc caattcttgc
     3181 aagtcttcat aaaagtctac gatctcatca ggcagatcgc catcatcgta tgtataagta
     3241 gctactacgc aaatatctgc ctcctcgaat tcatctgcat ctacttgggt acattcgttg
     3301 atttcgactt cgatatccag attttcaaat gcttctgcaa caatatcggc gatttcttca
     3361 gtgtttcctg tcatgctagc atatacgatt tttgctaaag ccatgatgtg cctcctcgtt
     3421 tataattatt taacaattat gaattatacc aaaacatcat tgaaaagaaa ataaaaccta
     3481 aagacacctg gggaatgtca tttggctaaa tgtatgccgc tatgttaaaa tgagagggat
     3541 ttgtaaaaag gagacgaaaa tatgattact ttaaaatcag cacgtgaaat tgaaatgatg
     3601 gcagaatctg gagctttatt agcagatgta cataaaaatc tgcgtgattt tataaaacca
     3661 ggaatcacaa gttgggatat tgaggtattt gtcagaaact atatcgagag tcatggcgga
     3721 attgctgcgc aaattggctt tgaagggtat gaatatgcga cttgtatcag tataaatgat
     3781 gaaatttgtc atggattccc tcgtaaaaag cctttgaaaa acggtgattt ggtaaaagtc
     3841 gatatgtgta tcgatttgaa aggcggcatt tctgattctt gttgggccta tgtagttgga
     3901 gaatctacac ctgagatcga ccgattgatg gaagtaacga agaaagcttt gtacatcggt
     3961 attgagcaag cacaagtagg gaatcggatt ggtgacatcg gtcatgctat ccaaacttat
     4021 gtagaaggcg aaaatctagc gatcgttcgt gattttattg gtcatggcgt tggtcctacg
     4081 attcatgaag aaccagcagt tcctcattac ggagaagctg gaaaagggtt gcggttgaaa
     4141 gaaggcatgg ttatcacgat cgaacctatg gttaataccg gaacttggaa aatgaaaatg
     4201 gatcctaacg gctggactgc gtatacaaga gacggcggac tttcttgcca atatgaacat
     4261 acattggcta ttactaaaga tggtccaaga attttgacat cacaaggaga agaaggcact
     4321 tattgatcta tccagtcaat aggtacggga gaggtacatg aaagtttgga acaaaataaa
     4381 acaaaatgaa aatctgatgc gctttattga gacgactcaa aagcgcatca ctgattcaga
     4441 aatgagtacg acctctgtcg tcgttgctta ttatttactc ttgtctttat ttccattgat
     4501 tattgctttt ggtaatattt taccgtttct ccacatcgat caagagactg tactgaccta
     4561 tttacgagaa gtgattcctg agaccgttta ccagtttatc gttcctgcaa tcaaagacct
     4621 tttgacacaa agttcaggag gcttgttatc cgtttctgca ttagcggctt tatggtctgc
     4681 aagtcaaagt atcaatgctt tgcaaattgc gatgaataaa gcgtatggcg tagaaaatag
     4741 aaaaaatttt ttgatcgtcc gtttcttctc actaattgtc attgtcttgt tcatgattgc
     4801 tattagtggg gtaactatcg ttcttgggtt gggacagctg attttggatg ctattcagcc
     4861 aatcgtacaa atacccgtga actttatcga tcagttccaa actttgaagt ggccaatcac
     4921 attggtagct ttatttgtca tcatgttctt gatttattta attgtcccta atgctcagtt
     4981 gaagttgaag acgatcattc cgggcacgat cttcgctact gtcggctgga tgctgttatc
     5041 acaagtgttt agtatctatg ctaggtattt tgcaacgagg atcagcggtt atcagatcat
     5101 aggaagtttt attgtactca tgctttggct taattttgct gctacgatca tcattttggg
     5161 cggaatcatc aatgcagttg ttcaagaata cgtgacgggt catcaaatca aaaaacgtcg
     5221 agatccaagc cgaaaatggt tcaataaatt gaaaactaaa ttttctcata aaaaaaagta
     5281 gaaacaagag ttcttattgt aaatttatat cagtaattta taataattat gactcttaat
     5341 taaagtaggt gcccatatgc ttgtttcttt tagtatggtt ataagatttt ttctgacaat
     5401 tttattatca ctgatattaa cacctgtatt caaaatcatt tcagtacaaa caggtatggt
     5461 cgacaaacca aatgaaagaa gaataaataa agttccaatg ccttcagcgg gcggactgcc
     5521 tatctttatt tcatttgtta tatcgacttt attcttattc cgtaaaatta tccctcaatc
     5581 gtatattgta ccgatcttga ttgcttcttt agtaatcata gtcacaggag tgctcgatga
     5641 taaatatgag ctgacaccta aacaaaaaag tatagggatc ttgattgctt ctcttattat
     5701 ttattttgta gcagatatac gtattgattc cttcacgctt ccttttattg gatatatcca
     5761 actaggctgg cttagttttc ccgtaacgat tttttggatc ttcggtatca cgaatgcggt
     5821 caatctgatt gacggtttgg atgggctggc ggcagggatt tcattgatcg gtttaattac
     5881 gataggaatc attggatatt ttttcctcca tgcttctaca gtgtatattc cagttgttat
     5941 tttctgtctt gtagcaagta ttatcggttt ttttccttac aatttttatc cggcaaagat
     6001 ttatttaggc gatacaggag ctctattcct agggtttatg atggccgtcc tttcactaca
     6061 agggctgaag aacgtgactt ttgtttcatc gatttctttg ctgatcgtca tgggagtgcc
     6121 ggtaacagac acgttttttg ctatcattag gagaaaagca aaccgtgttt ctttttctac
     6181 agcagataaa aaacatcttc atcaccgact aatcagttta ggctttactc ataaaggtgc
     6241 tgtcctgacg atttatgcga tggctttgat gttttctttc acagctatgg tcatgaatta
     6301 tactggaaga atcggaacga tcatattgat tattgccatg ttgtttgctg caattttatt
     6361 atttgaattg attggtctga tcaatgagaa acatcagttt attttaaaaa cattgcgatt
     6421 tttagggaat aaagagtacc gtacgcaagt aaaacagaaa tatttgaaga aatggaaccg
     6481 tcatatagat gaataacaaa ttaaaccagc cgatccttat tagtattgtg acggcaaaca
     6541 gtgataaaat ttttcggaca ctagataatt ttatttcaac tgtaaaagat gaaacagcag
     6601 taaaaatccg aatttttgac aacaattcac ttcccgagta caaaaaaaga ctaacagagt
     6661 ataggaaata tccctacatc gatatctatt ttcatgatga aaattccgga tttggttatg
     6721 gacataatca caatctgata gctagcgatt ctcgctatgg cattatttgt aacccagata
     6781 ttttagtgga tgaagagaca gttacagcat tggtcttgtt attgaaagaa catccggaat
     6841 gtgcgatgtt agcacctaag attcttaatg aagatggaac aactcagcac ctcatccgtg
     6901 agcagctggc tgtttttgat tacgttttgc gctttgtgcc tttcaaatgg gtcaaaaaaa
     6961 agtttgacaa acgactggct gtgtttgaat gccgaggatt gcctactgat gctaatagtt
     7021 atgtacggat gatctcaggt agttttatgg tagctgatat tgataaattt aaagagataa
     7081 aggggtttga tgagcgtttc ttcatgtact ttgaagacaa cgacttgtgt ttgagttttg
     7141 aaaaagcagg ttacaagtta ttgtacacac ccttttatgc agttatccat ttttacggga
     7201 agggtgcgca tcgaaatgca aaactgtttc gcgtgttttt gacttctatg atgaaatttt
     7261 tcaataagtg ggggtggcgt tttttctgat gaaaccagaa atattagtag tcgttgtgct
     7321 ttatcagcaa ctattcagtc aatctccagc ttatggacca ttaagcaaag cacttagcga
     7381 aaagcaaatt cagcttatta tttacgataa tagtcctcaa aggcagcaaa atgagctgtt
     7441 tgaaaaagaa aatatcgttt atcatcatga tccgcaaaat cctggtttag cggtggctta
     7501 taattttgcg ttgtctcaag cagatgaagg aacacgttgt ctcgtcactc tggatcagga
     7561 tacgaggcta gtcgacgatt attttgaact attgcgtaaa gtaaccttta cagatgaatg
     7621 tgttgctgca gttccgatga ttttttctgg gagccgacaa gtttctcccc tatattcaga
     7681 tcgttatatc aatcgtacag ctcaagcagt agaagttgaa ggcccaacag ctgaacgaat
     7741 catggcaatc aattcaggtg ttgcctggtc tgtcaaattt ctaaaaaaga ttggcgggtt
     7801 taatcctgct ttttctttag attttttgga tcattggctg ttttggaaag tggatcagtt
     7861 ggaaaaaact gtggagatcc ttcctgtacg aatggaacat gatctttcgg ttttagacta
     7921 tgaaaaagtt tcaagtaatc gataccgttc tattttacaa gcagaaaatc gattttatca
     7981 tgaatatgat caaaggcatt tatccagtca ccgacgacaa ttattgttaa gaacagctaa
     8041 acagttcttg accgtcaaaa accggcatat ctggcggatg actttgcgct catatgttaa
     8101 gaattggaag gtgtgaattc gtgcgttcgg tttgtatagc tacctttaat ggaggcagat
     8161 atttaagaga gcagttagat agtattttat ctcagctttc agcagaagat gaagtagtca
     8221 tttctgatga tgggtcagta gatgatacat gggatatatt aactgaatac gcagaaaaag
     8281 ataagcgaat ccagcttttt tcggggcctc gtcaaggatt gatcgctaat tttggttatg
     8341 cgatccagca gacgaaagga gatctaatct ttttggcaga ccaagatgat gtttggttgg
     8401 agacaaaagt gaaaaagatc actgactatt ttgagcagaa tccggatcag ctagtagtag
     8461 tttctgattt aatcattgtg gacagcgagc tgcaagagat ccatgattcg tatttttcgt
     8521 ttcgaaattc caaagaagga tggcttaata atgtgataag aagtcactat attggtgctg
     8581 gtatggcttt ccggtcttct ttaaaacccg atattttgcc gatcccgtca gaagtgccga
     8641 tgcatgatat gtggattggt ttgctgggag gaaagaagac tgggttccta agagaaccgt
     8701 taactctgta ccgaagacat gatttaaacg cgagtgagat tgaaacacat tctaaattac
     8761 ggcaaaaaat ttcttggcgt gttcatctga ttacggcgtt gatcaaacga aaatggttta
     8821 aacgttgata aaagatgttt aaatccttaa tatttccatg acaaatataa caattgatga
     8881 gataataaca aagtgaaatt tgaaatcaaa aaggagagca gatatccatg aagggaatca
     8941 ttcttgctgg gggcagcgga acacgtctgt accctttaac aaaggcaaca tcgaaacaat
     9001 tgatgccgat ttatgacaaa ccaatgattt attatccaat gtctactctg atgttggcgg
     9061 gaatcaaaga aattctgatt atttcaacgc cacaagatac accacgtttc aaggaattat
     9121 ttggaaacgg aaatgattta ggactgcaga tcgaatatgc agtacaagaa agtccagatg
     9181 gattggcaca agcgtttatc attggagaag aatttattgg agatgatagt gtctgcttgg
     9241 tattagggga caatatttac tatggtggcg gtctgtctaa aatgttgcaa cgagcagctt
     9301 caaaagatac aggcgcgaca gtatttggct atcatgtcaa tgatccagaa cgctttggtg
     9361 tagtagaatt tgatgagaat atgcaagctt tgtcgattga agaaaagcct gctcagccaa
     9421 aatcaaatta cgctgtaact ggcctttatt tttatgacaa tgaggtaatt tctatcgcta
     9481 aaggtatcaa accttcagaa cgtggagaac tagagattac ggatgtcaat aaagcatatt
     9541 tggataaagg aaaactatcc gtagaactta tgggacgcgg atttgcttgg cttgatacag
     9601 gaacacatga atcattgctt gaagcatcta cctttatcga aacaattgaa aaacgtcaga
     9661 acttaaaagt ggcttgtttg gaagaaattg cttaccgtat gggctacatc gataaggaac
     9721 aattgctggc ccttgcacag ccattgaaaa aaaatcaata cggtcaatat ttattgaatt
     9781 tggccgctga ataaggagtc cttacagatg aaagtaacag atacaaaatt aacagatgtc
     9841 aaaatcgtag aaatggacgt ctttggcgat catcgcggtt tcttcacaga aagttattca
     9901 aaagcaaaat ttgcagaaca tggattgaaa tatgactttg ttcaagacaa ccattcacta
     9961 tcagctgaag ctggtgtcct tcgggggctt catttccaaa aaggagaagc tgcacagaca
    10021 aaattgattc gggtagtcac tggagcagtt ttagatgtag tagtggatat ccgcaaaggt
    10081 agcccgactt atggtcaatg ggacggattt atcttatctg aacataatca tcgacagttg
    10141 cttgtaccta aaggatatgc ccatggattc gttactttga caccaaatgt gaacttcctt
    10201 tataagtgtg ataattacta taatgcagaa gcagatgggg gcatcgcatt tgatgatcca
    10261 gatttagcga ttgattggcc aattgatcca tcaagagcga ttatgtcgga aaaagatcac
    10321 cgtcatccga cattaaaaga atttgaagca gaaaatccat ttgtttacgg agaaatctaa
    10381 gaattaagga gaaattttct tatgaaaaac atcattgtta ccggaggtgc cggattcatc
    10441 ggctctaatt tcgttcatta cgtggtaaac aatcatccag aagtgcatgt cactgttttg
    10501 gataaactga cttatgcagg aaacaaagag aacttggccg gacttcctag cgatcgcgta
    10561 gaattggtcg taggagatgt tgcagatgca gaattagtgg accgtctagt aaaagaagcg
    10621 gacgcagttg ttcattacgc ggcagaatcc cataacgata attcattaaa agatcctttc
    10681 ccatttgtac aaacgaatat cattggtacg tatacacttt tagaagcttg ccgcaagtat
    10741 gatgtacgct atcatcacgt atctactgac gaggtttacg gcgatcttcc attacgtgaa
    10801 gacctgccag gacatgggga aggcgaagga gaaaaattca ctgcggaaac accatataat
    10861 ccttcaagtc catattcttc tacaaaagct ggttcagatc tgttagtaaa agcatgggtg
    10921 cgttcttttg gcttacgtgc aaccatttca aactgttcaa acaactatgg tccttatcag
    10981 catattgaaa aatttattcc tcgccaagta acgagtatct taagcggtat tcgtccaaaa
    11041 ttatatggcg aaggaaagaa tgttcgtgac tggatccata cgaatgatca ttcttcagca
    11101 gtgtggctga ttttgacgaa aggcagaatt ggcgaaacct atctgatcgg tgcagacggt
    11161 gaagaagata ataagaccgt gatggaattg atcttagaaa tgatgggcca accaaaagac
    11221 gcatacgacc atgtaaacga ccgcgcaggg cacgatttgc gttatgcaat cgattcgaca
    11281 aaattgcgag aagagctagg ttggcagcct gagtttacga acttccgtga tgggttggct
    11341 gacacgatca aatggtacga agaacatgaa gactggtgga aaaaagaaaa agaggccgtt
    11401 gaagcagcat atgccaaaaa tggacaataa tacagaggaa atttcactgt attaatggaa
    11461 aaacacatgt gaggcaggcg ctcgcctgtc tcttttttaa aataaaaatc aaggagcgga
    11521 taaccatatg tttttattaa ctggaggaaa tggacaatta ggtacagaat tgcgccattt
    11581 attagatgaa aaaaatctgc catatgtttc aacagatgca aaagacttgg atatcacaga
    11641 tgctgagaaa acaatggctt atattacaga attaaaaccc gatgttattt tccattgcgc
    11701 agcttacaca gcagtagata aagcagaaga agaagcaaaa gaattagatg agaaaatcaa
    11761 tgtcgatggc acaagaaatg tagcacttgc agcaaaagct gtgaatgcac aattcgtcta
    11821 tatcagtaca gactatgttt tcgatggaaa aaacaaaaat gtagaatacg aagtagacga
    11881 ccaaacgaat ccactaaatg aatatggtcg tacgaaatta ttaggcgaaa aagcagtaga
    11941 agagatttta gaagactatt atatcatacg aacttcttgg gttttcggga tctatggaca
    12001 caatttcgtc ttcacgatgc aacgcctagc ggaaacccgc gacaaactga ctgtagtaaa
    12061 tgaccagttt gggcgaccaa cttggacacg tactttggca gaatttatgg tatatgtcat
    12121 cgaagaaaaa gcaccatttg gtatttatca tttgtcgaac gaaaatagct gttcttggta
    12181 tgagttcgca aaagaaatat tgaaagatac agatgtagaa gttgcacctg ttacgtcaga
    12241 agaatttccg caaaaagcaa cacgtccgca gtattcagtc atgagcctga aaaagacaga
    12301 agcgttaggt tttgtcatac caacttggca agaagcactt gcgcaaatgc tggaagctgc
    12361 caaaaaaatg aataagtaat ttccttttta tcaaatagaa tttggagcat gatatggaaa
    12421 aacaaacaag aagtgcacga cgaaaagaaa agccaaaagc gaatcaggca gactcgaatt
    12481 tggtgcttga cctgtttcta aaactccgct atcttattgg cgttttagtc atcgtggtca
    12541 tcgttacttt taatttaaac ggaagttcac ttggtgtttg ggataaatat gtgtcgcaaa
    12601 gagacgatgg gaaaaaaact gatgtgattt ttggacagaa tcgggaagtg cggtcagacg
    12661 aatggctggt gcagacacct ttttacctct cacaagctga agaggacttt tcgttggtga
    12721 acgaggatta ttcattaaac ggacaaaaca tgatcattgc gtataattct cctgtaaagg
    12781 atattacggt tatcggaaaa ccattcaact ggggattctt tttcctcgga agagatcgag
    12841 gactttcctt ttattgggcg atgaaattag tcgtcatggt cttgcttgga ttcgaagtac
    12901 tgatgatatt aacgaagagg aaaaaagctc tatcgctaat cggtgcattt gcgctgacat
    12961 tttctccagc agtccagtgg tggttcatgc aacatgtggg cgatttgatt ttctttactt
    13021 tgggactgat gattgctttt tatcattatt tctacaatca tgataaaaaa tggctacgaa
    13081 cgttgatgat gcttttggtg gttattttcg gtattgggtt tattttagtg atttacccag
    13141 cccatcaagt gatgctggcg tacttgctga ttttttattt tgttggcctg cttatttact
    13201 atagcaagaa agtttcatgg gatctgttgg atgtagtgtt gattggaggg gcagttttat
    13261 ttattggcgg agtcatggtt catttctggc taacgtcaaa agacgcattg ttagcttctt
    13321 tgaatacctt atatccagga aaacgggtaa gtactggagg aaagtggacg attggcgaat
    13381 ttttttcttt ccttacaaat tggaaaattc cttttgaaga tatcaactat tcgaataact
    13441 ctgaagtggc cctgttctat cacttttttc caactgtatt cctagcaagt ccatttgtgt
    13501 tatttggcaa aaaagatagc gaacaaaagt tactgggacg tattttgatg attttttgtc
    13561 tgttttctat cttttggatt accgttgggt tgccaaaatg gctagcagaa atcactctgc
    13621 tgtcttatgt tcctccatta agagccatcc ttacttttag ttttgcggct tgtttattat
    13681 cgatctggtt tatcgcttat atttggagag aagatgttat tcctatttgg tataaattca
    13741 ttttgttggc agtaaatgca tggtcttatt tctatgcgat tacgcattcg aaaatggata
    13801 attatttttc ggattttgaa accatcgcag ttgctgtgtt gggaacagtg attttagctt
    13861 ttgcattatt tggctggaga cgcttgtttt acctcgcttt tgtcgcctta gttatcgtat
    13921 ctggcttttt cgtcaatcca gtcgtagaag gtacaggagc gatttttgaa aaaacactgg
    13981 caaaagaaat ccaaaaaatc gatcaacaag atcctggtaa agtttggttg tctgaagatg
    14041 aattatataa cttcacccca acattgggag tgaaatcctt caatacagta cgcttttatc
    14101 cagatatagc tgcatggaag aaaatcgatc cgaaagaaga atacgagaag tattataatc
    14161 ggtatgctca tacaagagct tttatcgcta aggaagatac aaagttcatt ttagatcgtc
    14221 cagatatgtt ttcggtcacg ttgaattttg aagacgcaga aaaattaggg atcgactatg
    14281 tgatcagtaa acaaccatta actgattaca accaattata tgaagcgcag ttcacgcagc
    14341 tatatggacc ggataaagac ggttatatga tttttaaagt agaatatccg gattatccag
    14401 atgtaccaca aacacaaact caggaaaatc agctagtcgt acagtaatag gctagctgag
    14461 aataatcctg aaatattagt agaaaggaga gtgaagataa ttcaagcaat cgtacaaaaa
    14521 tttgtcaaca aaatgagtgt cggtttcttg cttttgttgc tgacgctagt cttttttgtt
    14581 tggacagcag gggtaggttt tccagcaact ttgtttatat ataacaatag ttcattactt
    14641 atcgctttta gtgtggctat tttgctcatt ttaaatgttt ataaagcaaa aggcgtcgat
    14701 tttctttatc taggtgttgc gcttgccctt ttcattttct tttcttactt tcgggaatta
    14761 agaaatgagt ctttatttgg agacttactg tttcctttga ttattttatt ttgtttagta
    14821 attaaatgga tcgagtggac aaaatgggat cgcggtattt atctgattgt ctttacggtc
    14881 ttcctcggaa tcacgttata tcgtgtattt acagagatcc aagtaccaga aggacaaagc
    14941 atctgggctc caggcaattc gctttctgat atttggatca atacgaatac aatcggaagt
    15001 tctttgatga ctcttgcttt attgatcagt ggatttgcca gttcttttga acgatggtat
    15061 gttcgaatca tcggtgggat agcagtcgtt gcagcctttt taggtatttg ggtctgccaa
    15121 tctagaggtg cattagtcgc gttgattgtt tttgcgatat tggatctttt acctaaatcg
    15181 ttgatgcgtg tcattcgtgc gccatttatt gcctacacgg ttacgattct tttagctttg
    15241 ccgatttctt atctggcggc tgtctctgaa aaagtcaatt tgtttaccgg tcgagaagat
    15301 atttggcata aattctatca gacgctagga gaaaaatctg aacaagtttt actaggaatg
    15361 aaaacattta tcttccagcg aggaaaccaa tttttaggaa accacaatag ttataattct
    15421 attttgaata tctatggatt gataggattt gggattgctg ctttgctgct tatccttttt
    15481 attggtcgct taacgttgaa agcagattta tccaacgggc agatgacttt tatctgggcc
    15541 ttttttgcgg tgatgatgca gtcatttatg gaggatacat tgacttcata cccatgggtt
    15601 ccgattattt atatcctgtt ggctatggcc gttcatcgat atgatgaacc agaagttacg
    15661 gaggaattct atgttatgga agaggcatat gacgatatga cttatgatga tacatcttat
    15721 gaggaacaac cagatagcgc tcattcttcc aggatccaac gctatcattg ataggcacaa
    15781 aatacgagag aggctgtgac aaccgtcgca gccttttttg gtttattgca attgtctagc
    15841 atcttctgta aaatagaaac gttagagaaa atcttgtatc atagaaaatt aagaaagaaa
    15901 taagaacgca gctcggtacc aaaaatatgg taaaatggca cttgactgta attcactgaa
    15961 aatcgcttgg aggaacacaa atgtttaaag atttgtattt atttatgaag gacatttatc
    16021 agaacagaaa attactgcta cagttttcga tcaatgactt caaatcaaga tatgcaggat
    16081 cgtttttagg aatcttatgg gcgtttatca atccgctatt tactgtattg atttattggc
    16141 tggtatttgg tgttggactg aaagcacaaa tgatcgatgc aaaataccca tttatcgtat
    16201 atttagttac cgggatgatt ccctggttct tcttctcaga tgcatttgca tcaactacgg
    16261 ttgtttttcg ggaatatact tatttagtaa agaaagtcgt attcaatatt cgtatcttgc
    16321 ccacgacgaa aatcctatcc aatctgtaca cccatttatt cttcattctg attggcatag
    16381 ctgtcgcgct tataaatggt atatatcctt cagcaatgtc tttacagctg atttattatt
    16441 tattttgcat gtgtgctttc ttgacaggat tgacttggct gacggcatcg atccaaccat
    16501 ttttacctga tatcatgcaa ttaattacga tcgtgctgca gttgatcatg tggacattac
    16561 cgatattatg gagccctaat cagtttgatt caggtcttgt aagcatattg aaattaaatc
    16621 cgctgtacta tgttgttcaa ggttatcgtg agtcattttt aagtgaagct tggttctggg
    16681 agcatggaac atatactttg tatttctggg ttttcacgtt agtaatgttc gtaatcggct
    16741 ctgcagtgtt ccgtagattg aaaccacatt tttcagatgt gctgtaagga gaatagctat
    16801 gacagaatat gcaattagac taaaagatat cacaaaatct tataatatgt acgcaaaacc
    16861 ttcggatcga tttcgtgaag ccctcaatcc ttttaagaaa tcctatcatg atgtatttta
    16921 tgcgttgaaa aacgtgaatg tagaaataaa aaaaggtgag atgatcggtt tcgtgggaga
    16981 aaacggatcg ggaaaatcta caatgttgaa aatcattacg ggagtactga caccaacatc
    17041 cggcacgatg gaaatcgaag gaaaaatctc agctttgcta gaactaggat caggattcaa
    17101 tcctgagtat tctggctatg aaaatatcta tttaaatgga atggttctag gattttcaag
    17161 agaagaagtc gatgaaatgg tcgatgatat cattgaattt gctgacatcg gcgaccatat
    17221 ttatcaacct gtaaaaacct attcaagtgg gatgttcgtt cgtctagctt ttgctgtagc
    17281 gatcaatgtg aatcctgata ttttgattgt ggatgaagca ttggcagtag gagatcttga
    17341 atttcaacta aaatgtatgg aaaaatttac agaaatcaag aactcgggaa agacgatttt
    17401 attcgtttct catgatatta gttcgattcg ccgtttttgt gatcgttcct attggttgaa
    17461 aaatggaaaa gttgtcgaat atggtgatac gatggaagta acgacgaatt acgaaaactt
    17521 tttgaagaaa aaatcagtta aaacagtaga tcgaaataaa aaccaagtag aacgcttcgc
    17581 agctccagat atcgtagacg tgacgaaagc cgaattgttg gataaagata aaaatccagt
    17641 ggatatgctg gaacaaggag aaaaactata cgttaaagta acctatgaag taaaagacga
    17701 tacaatcaaa aatccagttt taggaattgc tctgcgtaca gtcgacaatt tttatgtgtg
    17761 cggactcaat actctactag atggtatcaa gatcccttgg aaaaaaggtg agaatatctt
    17821 ttatctggag tatgaaaagt taggtttgtt agctggtgaa tattattttg acgttgcggt
    17881 ctttgaagaa aatgcaacgg ttcctttggt atacaagaca aaatacatga acttgtttgt
    17941 cagcggttct tatatcggag aaggcatcgt ggtcttagac cataaatggg aagaagggac
    18001 tcatagcaat gaaatatgat tttgaaatgg acctagatga acaaagcagt gtgggaaaaa
    18061 ttgccgcaca gatcaaacca ggaagtaaag ttttagagtt tggtcctgga aacggccgat
    18121 taacgaaaca tttgatcgga gcaaaacaat gtgaagtgag cattgtcgag ctagataagg
    18181 aattgttcga ttttgtcagc gagtttgccc aagatggatt ttatggagat atcgaaagtt
    18241 ttgaatgggc aaactattat gctggacaaa catttgatta cgttttgttt gccgatgttt
    18301 tagaacattt ggtagatcct ggaaaaacgt tgaagaaagt tagagaattt ttgaatgaag
    18361 aaggagaaat tctaattaca ttccctaacc ttgcgcataa ttctgtcatg attgatttgt
    18421 tcaataatca attgccatgg gcttcttatg gtttgctaga tgaaacccat aattcttttt
    18481 atactcatga tggcttccaa aaagtttttg aaaaagcagg attatacatc aatattgaag
    18541 attatttgta cttagctgta ggtgatacag aattaaaatc aacttatgaa gaattgccgg
    18601 aagctgtacg ttatgatttc aaaatgcgac catttggcga agtctaccaa tacttcttct
    18661 cattgatgaa acatccagta gcacaaagtt ccattgcgca gccgcaaaat tctaattatg
    18721 tgaaagtatt agaagtaatc cagcaaacga aacaagatga aataattcaa caaataccat
    18781 tcaataattt cacaggggaa aatgagacac taagtttcca tgtatcagaa ttagcagaac
    18841 gaatggtgtt tcgcttcgcg cagcaaccta gctttatcga attcagcgca gaagctgctg
    18901 gagaaaaact cacatttatc gattcaaatg ctgtagtcaa aacagtaaat gattgttatt
    18961 tgtttgatgg taaagattta ccagagttct ttttaacgga tattgcaggg aaagaagtga
    19021 ctatccattg tcactatcgt tttatcggag aattgacacc aacaatgaaa gaattgctgg
    19081 aaacaatccg tccaatggct gaagttacaa agcagctttc tgagaaaaat gatgaattag
    19141 aaagagaaaa tcattatctt attgagaata atacaagatt agatcagacg ttgaaaacaa
    19201 ccataaatcg ctattgcacg ttgttggaaa gtgatgaatg gacaatcaaa catagattag
    19261 ggcgacgtaa gaaagaaaca tcgaaaaaaa tccaagaaaa agagctttct ctatgtattg
    19321 acgaaaaaat ctgggatgca gaaacaaaga tattgaaaat catcgggtgg ggaatcgcta
    19381 attcggatcg ccaaccattg tcctataagt tatcagccga ccaatcacca ttttttgaag
    19441 caattcctgt tttacgagaa gaagtcaatc aagcaaaaca gctagctgaa ggagcaaaag
    19501 caggatttga actgcggatc ctttgcgaga aggaacgttc attcttagtt gaagcagtgg
    19561 ctcaaaatgg tcaatcatgg atcattgaaa tgtaaataat gattaactta taaagataag
    19621 gagtaaacga atcgtgcaaa aaaaagaaat tgccgtattt atcgatcaaa tcactcgtga
    19681 acgggccact ggcgatttgc ttattgtggg ctgggcaatt gatgaagtga ccaaagaaat
    19741 ccctacgata aaggtagaaa aagaaaatgt gattgctgaa gcaacacatg tcgtaagatt
    19801 ggatatcaat catttatata atcttgatgt taagacccaa agcggtttta aggtacgttt
    19861 atcagggaaa atgagaggaa aagcaatctt agacttccaa acagcaagac atcaaaatgg
    19921 catagctgtg aaattaaacg gtaaatatcc ttatgatgac ggcattgaat cttcatggga
    19981 aagaaaaaaa agattactga aaaaagggat aaattatgca aggactcatg gcgtaaaaaa
    20041 agcaatccgc cgggtcaaac tggaattgaa accaggatcg atcgactatg cagagtggat
    20101 cagtcgtcat gagaaacctg atctgaaaga gcaaaggaaa gaagtacaag gatttgccta
    20161 ccgcccattg atttccatac ttatgcctgt ctataacgtt gaaatcaaat ggctcgaaaa
    20221 atgcattgat tcggtacttg atcaaacgta tgatcactgg gaactatgta tcagtgatga
    20281 tgcttctact gatccagcta tccgaaagtg tctagaatcc tatcaagcaa aagatgatag
    20341 aattaaagtc gttttccgac aagaaaatgg acacatcagt ctggcaacta attctgcatt
    20401 gaaaatggca gaaggagagt ttatagcgct cttagataat gatgacgaat tacctccatt
    20461 tgccttatat gaagtggcaa aagtgctaaa cgtacatcca gaactggatt tgatctacag
    20521 tgacgaagat aaaatcgatg cagatgggaa tcgctttgac ccgcacttca aagcagactg
    20581 gtctcccgat acattgatgg gaaataatta tatctctcac ttaggcgtat atcggacaag
    20641 cattgttaag gaacttggcg gtttccgtaa aggatatgaa ggctctcaag attatgattt
    20701 ggttcttcgt gtgacagaac aaattcctgc agaacacatt taccacattg accgtgtctt
    20761 gtatcattgg cggacgatcc cgggatcaac tgcaagcaat ggagaagcta aaagctacat
    20821 ctatgattct ggagtgaaag cattgacaga tgctctatct agaagaaata tcaagggaag
    20881 cgtacatcct ggcagaattt ctggttttta tgaaatcact tatgatgttt tgcaagaaga
    20941 cttggtcagt gtgatcattc caactaagaa tggctatgaa gatctaaaaa cttgtgtgga
    21001 ttcgatcatc gaaaaaacgt cttatccaaa ttatgaaatc attattgcag ataatggaag
    21061 tacagatcct aaaatgcaag aattatttgc agaatacaag catcagctga aagaccgatt
    21121 tatcgtcgaa ttaattgaca ttccattcaa ttattcacgt atcaacaatc tggcagcaga
    21181 aaaagcaaac ggcaaatact ttttattctt gaataacgat acagaagtga ttgaacctga
    21241 ctggatgaca gcgatggttt cttatgcaca gtttgatcga atcggctgtg taggagcaaa
    21301 attattttat ccggatgata caacgcagca tgcaggtgta ttattaggga tcggtggcgt
    21361 tgcaggacat gcattgaata actatgatcg aacacattgt ggttactttg gccgattggt
    21421 cattgacgtc aattacttag cagtcactgc agcttgtatg atggtcaagg cagctgactt
    21481 caatgctgtc aatggatttg atgaaatatt agaagtagca ttcaacgatg ttgacttgtg
    21541 cttgaaagtt tatgaactgg gtcgttacaa tgtctatgca catcaagcag aattgtacca
    21601 tttcgaatct aaatcaagag gttatgaaga tacaccagaa aaacaaagac gttttgctgg
    21661 ggaaatcaaa aaaatgcaag ataagtggcc ttcttatatt gctcatgacc cattttataa
    21721 cgataatcta actaaagaag gaatcggcga tttttcgttg agacctgatt gattaaaaga
    21781 actgtatttt catttttatt aatgaaaata cagttctttt aatttaatta tatgatttgt
    21841 caaattaagc tagaataaaa tgcttcttgt acatagctca aaaatatttc gattggtgtt
    21901 ctgtaattca atgattttct tggaatatga ttacgttgat tgctgacact ggaaataaat
    21961 gtctgattca ctt
//
LOCUS       DAXWLF010000030        21824 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXWLF010000030 DAXWLF010000000
VERSION     DAXWLF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.030"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(141..2216)
                     /gene="glyS"
                     /locus_tag="ACGE4U_000885"
     CDS             complement(141..2216)
                     /gene="glyS"
                     /locus_tag="ACGE4U_000885"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFF8393069.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2218..3135)
                     /gene="glyQ"
                     /locus_tag="ACGE4U_000886"
     CDS             complement(2218..3135)
                     /gene="glyQ"
                     /locus_tag="ACGE4U_000886"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFF8393070.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3519..4331)
                     /gene="recO"
                     /locus_tag="ACGE4U_000887"
     CDS             complement(3519..4331)
                     /gene="recO"
                     /locus_tag="ACGE4U_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFF8393071.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4474..5373)
                     /gene="era"
                     /locus_tag="ACGE4U_000888"
     CDS             complement(4474..5373)
                     /gene="era"
                     /locus_tag="ACGE4U_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFF8393072.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5388..5783)
                     /locus_tag="ACGE4U_000889"
     CDS             complement(5388..5783)
                     /locus_tag="ACGE4U_000889"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8393073.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5767..6243)
                     /gene="ybeY"
                     /locus_tag="ACGE4U_000890"
     CDS             complement(5767..6243)
                     /gene="ybeY"
                     /locus_tag="ACGE4U_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFF8393074.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6260..8452)
                     /locus_tag="ACGE4U_000891"
     CDS             complement(6260..8452)
                     /locus_tag="ACGE4U_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFF8393075.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8476..9447)
                     /locus_tag="ACGE4U_000892"
     CDS             complement(8476..9447)
                     /locus_tag="ACGE4U_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFF8393076.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10153..11532
                     /gene="brnQ"
                     /locus_tag="ACGE4U_000893"
     CDS             10153..11532
                     /gene="brnQ"
                     /locus_tag="ACGE4U_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFF8393077.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11686..12132)
                     /locus_tag="ACGE4U_000894"
     CDS             complement(11686..12132)
                     /locus_tag="ACGE4U_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFF8393078.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12159..12335)
                     /gene="rpsU"
                     /locus_tag="ACGE4U_000895"
     CDS             complement(12159..12335)
                     /gene="rpsU"
                     /locus_tag="ACGE4U_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFF8393079.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12497..12925)
                     /locus_tag="ACGE4U_000896"
     CDS             complement(12497..12925)
                     /locus_tag="ACGE4U_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8393080.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13017..13877
                     /locus_tag="ACGE4U_000897"
     CDS             13017..13877
                     /locus_tag="ACGE4U_000897"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFF8393081.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(13969..14319)
                     /locus_tag="ACGE4U_000898"
     CDS             complement(13969..14319)
                     /locus_tag="ACGE4U_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFF8393082.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14334..16061)
                     /locus_tag="ACGE4U_000899"
     CDS             complement(14334..16061)
                     /locus_tag="ACGE4U_000899"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFF8393083.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(16275..17201)
                     /gene="trxB"
                     /locus_tag="ACGE4U_000900"
     CDS             complement(16275..17201)
                     /gene="trxB"
                     /locus_tag="ACGE4U_000900"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFF8393084.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17346..18779)
                     /locus_tag="ACGE4U_000901"
     CDS             complement(17346..18779)
                     /locus_tag="ACGE4U_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFF8393085.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            complement(18779..20182)
                     /locus_tag="ACGE4U_000902"
     CDS             complement(18779..20182)
                     /locus_tag="ACGE4U_000902"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFF8393086.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            20449..21627
                     /locus_tag="ACGE4U_000903"
     CDS             20449..21627
                     /locus_tag="ACGE4U_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HFF8393087.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGAKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAV
                     YQASSKEEANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIE
                     SFNKQIKKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
BASE COUNT         6029 a         4664 c         3872 g         7259 t
ORIGIN      
        1 gtgtttttaa aagttacaca aaaaataaaa taacaaaaaa attaacattg aattaataat
       61 agttatttat atttataaca aataaaccag agaaatagcc tattttgtgc ctgttctctg
      121 gtttatttac atattagaaa ttattttgtg atgatttgat ctaaacttgc tagtgacaaa
      181 gccatggcag caatttgact taattgcgtt aatcggttct tacgtactgc tggttcttct
      241 gccatgacca tcgtctgatc aaaataattt tcgatcagtg ggcgaaggct tacaagctga
      301 tcataatttt cgttgatcgt ttggtcagca aagcttgttt tcattgcctc tactgctttg
      361 tgcagttctt tctccgcttc gttttcgaac agttcagggt ctacttctga ttgtttttca
      421 agctccgctt ttttcgctaa gttcacgaca cgtgtcaaag cttccatcga tggtttgaag
      481 tcttcatctg caagatgctg cttgaacaat tgtgctgctg caaatacttt tgtcagatct
      541 ttttgttctg cattcaaaac agcttcgatg acatcatgac ggatattctt cgttgtcaat
      601 aattggcgca gacggccttt gataaagtcg atcacctgtt gttgtcccgc tgacaagcca
      661 atgccaaaac gatccgtatc tttactgata acttcactaa tcgtttcttg cagaacagac
      721 aaaggaaagg cccatttttt cgattcgatg attcgaacga caccatatgc ttgacggcgc
      781 aaagcatatg gatcgtttga gccacttggg atcatcccaa ctgcaaagaa agaaaagata
      841 ctatctaatt tatcagcaat cgctaaaaca gaacctactg tagtctcagg taaatctcct
      901 tcactggata taggcatgta atgttcacgg atcgctgttg ctacggctgg tttctcgcct
      961 ttaagcagag catatttttc gcccatgatc ccttgtagtt cagggaattc tcctaccatg
     1021 ttggttacta gatcaaattt atagatttca gcagcgcgtt gcagatccat caattcttcc
     1081 tcagatagtc ctacttgttc gcctatgatg cgagcgatga cttgtacccg ttgcattttc
     1141 tcatagatgc taccgatttt ttcatggaaa gtaacagttt ttaatttttc aacgtattgg
     1201 tcgattgaag ctttcttgtc ttcattataa aagaattccg catcttctaa acgagcagtc
     1261 aatacttttt gattgcctcg taccacattt tccaatttta cccgatcgcc attacgagca
     1321 gaaataaaat gaggaagcaa cataccttga tcgttccgta cttcgaaata acgttgatgt
     1381 tctttcatag aagtgactag tacttcttca ggaacagata gatatttttc ttcgaagtcg
     1441 ccagaaaaga cagttggata ttcgactaaa ttgttgactt cttctagtaa atcttcatct
     1501 agatcaatga tccagtgatt tttttctgct aattcgtttg cttgtgtgat gatcatttct
     1561 tttctttttt gcggtgaagc aataacaaat tgttcattca atttttcttc gtattgtttt
     1621 gcatgcgtaa atgtcacatc ttcgcctaag aaacgatgac ctcttgaagc tctgtctgtt
     1681 ttgacatcca agatctcaaa cgggattatt tcgtcatcta acaatgccac gatccagtga
     1741 atagggcgaa tatattcaaa atcgtattct gcccagtgca tcgttactgg gaaagtgagg
     1801 cttttaatga catctgctaa acctgtcaat acttcttttg cttctttgcc gtgagtgtat
     1861 ttcgttacat agacatattc cacgcctttt aattctctaa aagtgatagc atctgtcgtc
     1921 agaccttgtc cgcggacaaa tccttcagct gctttcgtcc agtttccatc tgcgtccaaa
     1981 gcaatttttt tggctggtcc tttgacttct tcttcgctgt ctgcttgacg ttctggaatg
     2041 tttgttactt tgattgctaa gcggcgcggt gtagaaaacg tttcgatcgt atcataagtc
     2101 aattggtttt catctaaaaa ttttgctgtt ttttcttcta attgtttcat gcttggtgtg
     2161 actacatgag caggtatttc ttccagcccg atttcaagta ataagtcttt tgccatctta
     2221 ttcagcctcc ttcacgtttt ctggatttgc tgtgttttta ttcaataaag ggaatcctaa
     2281 tttttcgcgt tctgccacaa agattttggc tacagcacga gccatgttgc ggatacggga
     2341 aagataacct gcacgctctg ttacggatac tgcaccgcga gcatctaata aattgaatgt
     2401 atggctacat ttcaaaatat aatcatacgc tggatgaaca agattttcat cgatacagcg
     2461 tttagcttct ttttcaaaac gatcaaaatt gcttagtagc aggtcttggt cgcttacttc
     2521 aaaagaatat ttagaatgtt catattctgg ttgtttgaag atctcaccat atttcacgcc
     2581 atcagcccat tccagatcat aaacactttc aacttcttgg atataagaag caagacgttc
     2641 tagtccataa gtgatctcag aagtaactgg tttacaaggt agtcctccga cttgctggaa
     2701 gtatgtgaat tgtgtgatct ccatcccatc aagccatact tcccagccca caccggcaca
     2761 acccatcgat gggttctccc agttgtcttc aacaaagcga atatcgtgtt ctaatggatc
     2821 aattcctaaa agacgcaagc tttctaaata aagctcttgg atattttcag gtgaaggttt
     2881 cattacgact tgaaattgat gatgctgata caaacggttt gggttttctc cataacgccc
     2941 gtcagctggt cggcgggatg gctctacata agcagcattc catggttccg gtccgattgc
     3001 ccgtaagaat gtatatgggc tcatcgtacc tgctcctttt tctgtatcat aagcttgcat
     3061 cagcatgcag ccgtttgatg accaaaattt ttgtaatgtt aaaatcatat cttggacggt
     3121 caatttttta ctcatctgtt ttgccccttt caatttttaa gcgttgctgt caataagaat
     3181 acaggaaaga aaacgacaaa aagtccctat gtctgattac agacataggg acgaataatt
     3241 tcgcggttcc accctacttc cgaataaaga ttcggcagct tcatttatcg tactcgcaag
     3301 tgcctcttca aaaatcgttt gggtccggct tccactctcc cgaactcgct aaaccacgtg
     3361 ttttttactg atcttggtca atgtacgtat tcattttgca gttttctggt ttgtataata
     3421 gatcctctca gtgaacgatc tatttaatct acggttaata atagacggaa gtccctgtat
     3481 ttgtcaaata gttttgattt ttttgttgta tctttcattt attcttcttt ttcaggtatc
     3541 tctttttcag gcatattttt ctctggcgtc tttaatacgt gtgcccaaga cttcatttga
     3601 tcaatgaact ttttgctttt tagatgaata ccgacatatt catcatacaa ctggtcaatg
     3661 aggtcacgga ttgcctgctt cgtttcttct ttcaagtgga ttccactgat cttatcataa
     3721 gaaatagccg aaaacattcg aacgaaataa acagcgcgag gatctgcatg ataacggtgt
     3781 tcgtccattg gccagtgttt ctcgcatatc acaccgccat atttggaaga atagtcaaat
     3841 tttccttttg tttccccgca tacgacacag tgcgtccaaa ttggcgcgat tccgaagcgt
     3901 tgcagtattt gcacttcaaa gatattcgtg atgatttctg catcatctcc ttggtccatc
     3961 cgctgcagag cttgttgcgt aaattggaat aaatgcgggt catattgatg atcttcgatt
     4021 gctgcatcta ctagatttag caaatacgta ccataaccgc tcaaaaagat atcttcttgg
     4081 atgttccgaa acgcatggac gtctttgaca ctgcttaaaa acgacaaacc ttcacttcga
     4141 aaattaccga tatagactgc ttcggtaaag gggagcaaag ccggagctaa tggctgattt
     4201 tgccgatgga ttcctttgat ataaaacatc attttgccag aagattccgt aaagattttg
     4261 actaatttat ctttttcttt gtaattacgg ctagataaaa tcaagccttt cgattctgca
     4321 agctggctca tattctccct cctaaaaaat aaatctggaa aagcagattc tgccgatcca
     4381 gattatttat gtttcgatct tccgctgttt ttctcaacgt acggattttt atccagttta
     4441 tttctatacc taacctataa gaagtccaga atatcagtac tcgtcttttc ggtagccgaa
     4501 gtcttgcaga tagacttttt tgtctcgcca gtctttttgg actttgaccc aaagttctag
     4561 aaatactttg tctcctaaaa gattttcgat atcctttctt gctttcgttc ctacttgctt
     4621 cagcattttt ccgcctttgc cgataatgat tcctttttgg ctatctcttt cgacgatgat
     4681 cgtggcctgg atatggattt tatcattctc gtttcgcttc atcgaatcaa caacgactgc
     4741 gacagaatga ggcacttcgt ctcgtgttaa aaacaagact ttctcacgga tcaattcaga
     4801 aacgatgaag tattcaggat gatcagtcac ttgatcatct ggaaaatatt gtgggccctc
     4861 tggcatttca ctgactaata catccattaa gcgttcgaca ttgtttcctt ctgtagcaga
     4921 gatcggaatc acttgtgcaa aaggcatctg cgtcgaataa tcttcgatga ttcccagcaa
     4981 ctcgtctgga tggatcttat cgattttatt gataatcaga taaacaggag aatgcacgtt
     5041 ttttagacgt tcaataataa aatcgtctcc ccggccacgt ttttgatcag cactgatcat
     5101 aaataagact gcatccactt ctttcaacgc actgtaagct gcttctacca taaaatctcc
     5161 tagacgatgc tttggtttat ggattcctgg tgtatcgata aaaatcagct gagcttctgg
     5221 tgtagtatat actccttgga ttttatttct agttgtctgt gccttgtcac tcataatggc
     5281 aattttttga cccacgattc ggttcaataa tgtggatttt cctacattcg ggcggccgac
     5341 gattgcgaca aaccctgatt tatgttctgg catatacgtt ctccttttta attaaacaac
     5401 aaatgatata ttttagggac aaataaaatc aacccaatga tgacagcaaa acaagcggtc
     5461 actaaaactg ctccagctgc catatctttg atttttttcc caatcggatg aaaatgaaaa
     5521 tccgtaaaca tgtccactac attttcaaaa acagtattga tgatctctac tatccaaacg
     5581 agaaaaacag ctaaaagcag ccataaccac tctatccgat caagctggaa aatcacgccc
     5641 ataaataaag caaggatacc gaacgcaaca tgtttcctca tattgcgttc ttcctcaaaa
     5701 acggttttga ctccttggac agcaaattcc acagacatca ggaaatgttt gtttttctct
     5761 acgtttttat cttttgagtc cataggcatc caagatctct ttctgtaaac caaacatttc
     5821 tttctcatct tctggtgtca tatggtcata cccgttgata tgcaagaatc catgtaccgc
     5881 taaaaagcca agttcccgat catatgaatg gccgtaagct tctgcttgtt cagcagcacg
     5941 ttcaatagag atcatgatgt ctcctagtac acgaggtaat ggattctctc catcttcaaa
     6001 aatcactggg atttcgtctt cgccttcttc ttccagagca aaactgatga cgtctgtcgg
     6061 tgcatctttt ccacggtaat cacgattgat tacttggatt gccgcattgt ccatgaaagt
     6121 aacagacatt tctgtttcct ctggcagctt taaataagat gctgcaaatt gcagcaagtc
     6181 atcgatttct ttcattttct cttctgagac ttggtttgtt tcatccataa aggtaatatc
     6241 cattatacat cgcttctttc tattcttctt gttcttcctt tatcttctca acttctgttt
     6301 tcattttagg atatttgatc cgagaatgga aggtgctact taaaccgctg attaatgatt
     6361 tttccacttt tcggatttct ttcaaggtca gtccactatc gtctaattga ccatcggaaa
     6421 tacgctcttc gataagatta tggacaaaac tttcgatttt ttcgctcgtc ggatgatcca
     6481 ttgcccgtac tgcagcctca caactgtcag caatattgac gatccctgct tcacgacttt
     6541 gcggttttgg tccaggataa cggaattgct cttctgttac ttccggattg cgttctttcg
     6601 ctttgatgta gaaataacgc attagcgtgg ttccatgatg ctgtttgcaa atatcgatca
     6661 ccatttgcgg catattataa tcttccaata ttttggctcc atctgtcaca tgtccaaaga
     6721 taatatgttt actgtcttct ggcaataaga aattgtgcgg gttttccgct cctgaaggca
     6781 agttttcaac gaaaaagcta gcatgtttga tcttgccgat atcatgataa tagcaagcta
     6841 ctcgagttag caaagaacgt ccgccgattt ctgctacagc gttagcactt agattagcta
     6901 ccatcatact atgatgataa gtacctggcg cttcttccag caactgtttc aataaaggat
     6961 gatttggatt gctgagttca tttaatacaa ttacactgtc atcattgacc atcaattcaa
     7021 tatatgggtg tagaccgatc gtcagcaaga atgagaagag tgcaccagtg aacccgcaga
     7081 cgagcattaa ccaagtcgtt gcatcaccaa agctcattcc ttgatagacg atcaaaatga
     7141 catccatcat cacaggaaaa ataatgaccc acatggcagc agataatcct tgttctgaga
     7201 tccgtttgcg tctgacaatc gttgccatga gcccagagaa aacataagac atcaagataa
     7261 tcgttagaaa gttcgtgcca atcgaatcat aaaaaatgaa aagtgcggat actgcttgga
     7321 aaataccagc catgatccct gctcttcgat tcaagaaaaa gcttaaaaca agtggtgcaa
     7381 aagcagcggg ataaaacagc gggatataag tagcttgttc cgtttggaat aattggaaaa
     7441 atttcatcag cactatgctg actgacatag ctgtcacata gaaaagcacg tattttgttc
     7501 tttctcctgc attttggaat tgaagtgaat tataaatcag tacgccgatt tgaagcaaaa
     7561 cagctaaaac catggcaatt agcggaaaaa tcgaagttgt ttgattagtc agtcctagta
     7621 attccaattt tttcatagct gaagcatcga tttgattacc ttcacgaaca atgatttctc
     7681 cttggaagat catcactggt tgtacagctt ctcgtgcgga ttgtttcaat tcttctgtct
     7741 ttttctcatt caatacgtca ttcactacga taccttgatc cactaagtag cgaatcattt
     7801 gttgttgtgc tggtgtgaca ttcaaatatt ggatttgctc ttctgcttct tgtttaaaag
     7861 cttccaattc actttcacga acttgtttgc tcatctgttc gtcaatgagc ttcagactct
     7921 catccctcac agtatgaatc tcagtttcag tcatttcaaa tatcgtcgaa taaaaattgt
     7981 tcggtagttt ttgataaaac acaacatttt ctgcattcac attttcaaaa tttttcttca
     8041 gcgcggcgat ccgctcttct gtagtaggct ttgggatatt ttcatcttcc tttgccttat
     8101 caatcttttc gtcataagat ttgttgatgg catcattcgt cttatcgatc aattcaaata
     8161 gttgcttgat ccgattgttc tggtcatctg caagatcttt ttggtaagtg tattctggag
     8221 tgactgcttc tgctgccagt tttctttttt gttcggtttc ttccgtgttc tcgatcgttt
     8281 tgttcgcacg gatactttct tctgctactt ggccttctct gaaatcattg gccttttgtt
     8341 tgacactact aaatgtaacg ccgcagataa tgataaaaaa caagaacagc aggaaaggca
     8401 atgctgcctt tccaagtttt tcttgtagtt tctgcagtgt tttattcaac atgtgcttat
     8461 cctcctaagc aaagcttatt cttgatggag atctgccttt tcataagctt tgatgatttc
     8521 ggcaactaca gggtgccgaa caacgtcact tgcttcaaaa ttaacaaaat cgattttctt
     8581 gatttgcttt aatgttcgtt ctgcatgaac aagaccactt gtcgtccctc gaggcaagtc
     8641 tatctgactt gtatccccat tgacaatcat ttttgattga taaccgagac gggtcaagaa
     8701 cattttcatc tgagcgactg tcgtgttttg tgcttcgtcc aagataacga atgcatcatc
     8761 caatgttcgc ccccgcatgt aagctaaagg tgcgatttcg attacgcctc gttccatcaa
     8821 tcggttcgta tgatccaatc caaaaatttg gtataaagca tcataaacag gccgcagata
     8881 aggatccact ttttctttta gatccccggg aagaaagcct agattttcac ctgcttccac
     8941 agcaggccgc gtcaaaatga ttttttgcac ttcacctttt ttcaaggcag cgattgctaa
     9001 tactactgca agaaaagttt ttcctgtacc ggctggtcct acgccaaata cgatatcatg
     9061 tttggctacc gattcgacat atttcttttg gccgctgttt tttacccgta taggttttcc
     9121 attacggtct ttgacgatct cttcctcata catctcaaca aaataatcta atgtaccttt
     9181 ttgccccatt ttcaatgccg tcacaacatc gggcgtactt acgtggattc ctcgtttgat
     9241 caattcctgt aatgtccgaa tgactgaggc agccaaggaa atctgggatt gctcaccaat
     9301 gatctgaatg atttctccac gtgtatggat ggttgtttcc gttgtttcct caatcagttt
     9361 tatatgcttg tcgtgtgcac ctaaaagcat gctgacatca tcagcagccg tcaatctaat
     9421 ctctaaagaa ctagactctt ccgtcaaaaa tagacatccc ctttttttcg attcattctt
     9481 tcctatttta ccatactcag aagaaactat atagcaaata agaaaaggtt ctaaaagagt
     9541 tgcttaacca taagtaccaa gaaacgttaa aaaactagcc gcttccattc tctaaaggtc
     9601 tcgtttgaat aaaaaaagct gatcatgtaa gctatgcata agatagagga caagtattcc
     9661 gtctaactta gcaagtattc tctgggtatt tttcttgttt caaaaaaaga aaacaaaata
     9721 tttgaaatat tcttgcattt caaaaaaaac aggtgtatta taacgtcaac aaataaatat
     9781 acagcaaagc gtcgaggaaa acagtaggct attcagtgat ttattcccag tgagtcatgt
     9841 aagtgagaaa tgacaataaa tcgaatagac gaacatcatt tccgagtcaa tgtcgaaaac
     9901 gtagatcatt gtggctgcct ccattatcag gctagcggat aactttatcg aaaagcgtcc
     9961 gtgaatgaag aagctttttt aagcgaaaaa aggtggtacc gttctagaat ctagagccct
    10021 tttcccaagt ggaaaagggc tttttttata ttttaaggtg gtggttttca tggaatctga
    10081 tattagtggt atttctagtt tctgcaacgt ttattttgtt actcaaaatg ataattattg
    10141 gaggaaaaat ttatggaaga aacaaaaact tattcatgga agcaattatt gatggtcagt
    10201 tcacttatct tcgggatgtt cttcgggtca ggtaatctga tctttcctgt acacttaggt
    10261 caaatggctg gatcaaactg gatcagcgct ggtgtaggat ttgccatctc aggcgctctt
    10321 ttcccattat tagctatctt agctgtagta gtgacaaaaa gtgatggtct gtatgactta
    10381 gctaaacctg tcggaaaatg gtatgctgcc ttgttcttga tccttgttca tttaacgatc
    10441 ggaccattct ttggtacacc gcggacagca gcaactgctt ttgaaatggc tgctaaaccc
    10501 tttttgcctg aaaaatatga ttcattaggt atgtttttat tctctgctct tttcttttta
    10561 cttgcttatt tccttactgt acatccaaca cgtttggtca aatatgtcgg aaagtattta
    10621 aactccttgt ttcttgcatt attagctatc atttttgtcg tttctttatt ccaacccatg
    10681 gggaatctaa accaatctgc tgcagattct tatcagacga acgctttgac aaatggcgtt
    10741 ttagaaggat acaacacggt tgatgcagta gctttacttg ctttaagtgt cacttttgtc
    10801 catgcagttc gtgatcttgg ttttaaagat aaaaaagtca cagaattaac agcaaaagct
    10861 ggtagcctga gcgttgcgtt agaagtcatg atttattttg gtctggtcct tctcggtgcg
    10921 atgagtttga ataagatgtc tttatcagaa aatggcggaa ctgcactttc tcagatcact
    10981 aatttttatt taggtaactt cggtgctagc ttcttagggg taatggtcac attaggtgtc
    11041 ttcactactg ctatgggact cgttgtttct tttgcacaag atttccataa actgttccct
    11101 aaagtcagtt atatgacatg gctgcggtta acgacttttg tctcttttgt cgttgcgaat
    11161 gccggattgg ataatatcat ccaatggtct ttaccagtat tgatgctttt gtatccattg
    11221 tcacttgcgt taatcttact ttccttaaca gcaaaattct tccaaaagag tccatttgtc
    11281 tatcaagtaa cgatgttctt ttctgccgtt ccagccgtat tggatatgtt ggcaaattcg
    11341 cctgcattag tgagtcagca aactgtcgta gcaagcttac taggatttta tcatcattat
    11401 gttccttttg ctgcattagg tcttggctgg gtcgtcccaa cgttgcttgg ttacgctggc
    11461 agcttgctct tttattatgt atatcgcgta tcaggatata aacaagaagc aagtgagtta
    11521 ccagaagaat aaccaaatca aacacaattt aacataagag atattaaaca ggaaaaaaac
    11581 agctagaaga aagaataaga ttctttcttc tagctgtttt ttagttcttt ttttacgaac
    11641 acaaaaaact gcggataatc actgtccgca gtttaaatga taaaattaag attgtaatag
    11701 ttctttcaca atcgcattca cttggtttcc atctgctttt cctttgactt taggcataat
    11761 tgcacccata actttgccaa attctttcat tgatgaagca ccagtttgag tgattgcttc
    11821 ttgaacaagt tgtcgaattt cttcgtcgga aagttgttcc ggcatatatt tttcgactat
    11881 tacaatttca ctttttactt tttcagctaa atcgtcacgt cctgcttctt caaactcatg
    11941 caaagaatca cgtcgctgtt tcatttcacg agacaacacc gttagttctt cttcttctgt
    12001 caagtcgtga ccttctttaa tctgctcgtt ttgtatagat gacttgatca ttcggatgac
    12061 ttgtaaagat tccttatctt tcgctttcat cgctgctttc atgtcatcgt ttaatctact
    12121 tagtagtgac acactaccaa ctccaattca tcacagaact agaatttttt gcgttttcta
    12181 gctgcttcag attttttctt gcgtcttaca cttggttttt cgtagaattc acgtttacga
    12241 gattcttgta aagtacccgc ttttgacacg gaacgtttga agcgacgaag agcatcgtca
    12301 agtgattcgt ttttgcgaac gactgttttt gacatgttaa ttccctccct ccgagcttaa
    12361 aaagtaaccg taattgttat tgaaatatat ctcaaaaaac aagactgtcc tgtaaatatt
    12421 atatctaatg ctgttttgac cgtcaaggaa atcttgcttt tttttaaaat tatcaaatgt
    12481 tattttattt aaatccttat tgacaatttt cacatcttcc gaaaatctcg aatctatgcc
    12541 cttcgatcgt gcatccacct agctgttctt cgaaataatt catcggacac atatggattt
    12601 ctttcgttgt tccgcacact gtacagataa aatgatggtg atggccgatt ttcccctgac
    12661 agcaatgaaa acgaaacttc atttctccat ttaaatcggt ttcttccagt aaatcgatct
    12721 ctgcaaaatc atggagattc cggtaaatcg tatcataact tacccctttg aattcctgat
    12781 tcatatagtc aaaaacttct ttagcagata cgtagcggtt acgcttcact aaataagtta
    12841 acaatgcttc tcttttttta gtatatttaa aaccgttatt tttcaatgtt tctaatgatt
    12901 gttcgatcaa tgtctgtttt ttcatgactt attcctccaa atcaaaatga ttacgaagta
    12961 cgcattctag agtatggtat aataaacata aaaaaaatgc aagtgaggtt ttatccatgt
    13021 cttccgattc tacagaaatc gttacttttt ttgtcatctc cgactctgct ggagaaactg
    13081 cgacaaaact agcgcaagct actatggctc agtatcctac cgttgagttc aacctattcc
    13141 gcagaacgtt cgtgacagat gaaaaaacat taaggcaggc attgcaagat gcattagaag
    13201 aaaaagcact tgtcctgcat acattaatca atcaagaact gatcgagata acacgttctt
    13261 tttgtgaaga acatcaatta tttagttttg atgtgatgac gccaccgata tcagaaattg
    13321 aacgtttaac tggtatcaag ccaattcggc aacctggtgc cctccattta ttgaatgaga
    13381 actatttcaa acggatcaaa gcaatggaat ttgctgtaaa atacgatgat gggaaagatc
    13441 ctcgtggttt tttagaagca gatgtcgttt tgctaggcgt atcccgaact tccaaaacgc
    13501 ctttgagctt gtttttagct aacaaaaatt taaaagttgc caatttacct ttgatcccac
    13561 aagcacatat tcccaaacag ctttgggaga tcgatcctaa aaaaatcgtg ggactaacga
    13621 ataacccaga tattttaaat aatatccgta aagaacggat gcgctcttac ggattaaacc
    13681 ctgatactgc ttattccgat atcgaaaaaa ttagagctga acttgatttt gcgaatgatc
    13741 tttatgaaaa actaggatgt gtagtgatca atgtggcatc tttatctatt gaagaaacgg
    13801 cttcgcttat tttgaatgct ttagacctag aagatcatag ttattatgga actgaaacaa
    13861 cagaaaaaag cgattaatca tttttgtctt tttcttaaaa aacactggag tgtgacaacg
    13921 aagaaatttc gttgtcacac tccagtgttt atctatttgc acctatcgtt attgatgttc
    13981 cacatgtttg agtgtctggt tcaaaatatc cagtacatga gaatcatcaa gactatacaa
    14041 aatcgctttt ccctcgcgat gagacttgac gacatgattt tctcttaata aacgtagttg
    14101 atgcgataca gcagactgct ccatttccac tgcttgggtg attgctgtca cattttgttc
    14161 cccgtctttc agatatagca gaattttaag acgagtgggg tcactcaaca ttttgaataa
    14221 ttgactgact ttctctactt ttgatttttg cgtgtctggc aatccattca cctccaactt
    14281 gaaaaaagac tgacttctaa agtttaccac tttttccatc agtctcaagt tttttattct
    14341 tctgtcatct ttttgataat cgcttcataa gaggctactt tttcggctgc gtcttctgta
    14401 gagcttcctt ttacaccaat ataaaacttg attttcggct cagttcctga agggcggata
    14461 gcgatccagc cattttcttc caatgtatac ttcaatacgt cggatggcgg tgttgttagt
    14521 ttttctacgt ttccttcacg gtctgttctc gtcaattctt taaagtcttc tgtttgaata
    14581 acttttgctc cggcaaattc tgtcggtgct tctttacgga atttttccat caaagcagag
    14641 attttcgcag aaccttcttg tccgctcatt gtcacagaaa tcgttttttc tgcgaaatag
    14701 ccataggttt caaaaatcgt ttgcaagccg tcatacaacg ttttgccttg ttttttatag
    14761 taagctgcta cttctgctaa taaaagcaat gcttggatcg cgtctttatc gcgaacaaaa
    14821 ggtttaacta aataaccata actttcttcg aatccaaaca taaaagtatg tgaatggtct
    14881 tcttcaaatt gttggatttt ttcagcaatg aatttaaacc ctgtcaacac attgaacata
    14941 gtagcaccgt agtgttccgc aattgctgta ggcaattcgc tggaaacgat tgatttcaat
    15001 accgctgcgt tttctggtaa agtgcctctt gattgatgtg cttccaaaat atattgagca
    15061 aacaatgctc cgatttggtt cccagtcaat acttcataag aaccatctgg caaacgaacc
    15121 gctgcgccta aacggtctgc atcaggatct gttgcaatca gcaagtcagc tccctctttt
    15181 tctccaagtc gtatagcata ttcaaaagct gaatgttctt ctggatttgg cgatttgacc
    15241 gtagtgaaat ctggatctgc cacagcttgt tcgggaacta gtacgaattg ttcgaatcca
    15301 gcttgtttca acgcacgttc gcccaacatt ttccctgttc catgaagtgg cgtgtacaca
    15361 agtttcaaat ctttccccat tgtttcaacc aattcacgat caatcgtcac tgctttgatt
    15421 tcttctagat aagcagcgtc cacttcttct ccgatgatcg tgatcaaccc gctatgttta
    15481 gcttcttcgt cagataatac ttcgactttt agcggattgc tgacttcacg gacatatttc
    15541 gtcaatgcat ctgcgtctgc aggaggcatt tgtccgccat cttctccgta gactttatat
    15601 ccattgtaag ccgctgggtt gtgagatgcg gttaccatga ttcccgcaaa cgtcttcaaa
    15661 tgacgaactg caaaagaaag ctctggtgtt ggacgcagac tttcgaatac ataagacggg
    15721 atattgtgat gtgccagtgt tttggctgct tccattgcaa attctggtga ctgatggcgt
    15781 gagtcataag cgatagcgac acctcggcgt tttgtttctt caccttgtgt atccatgaaa
    15841 cgtgctagtc cttctgttgc ttgtcttacc gtatagatgt tcattcgatt gatcccaggt
    15901 cctaagattc cccgcatgcc tgcagtaccg aattccaacg gtgcataaaa agcatcttca
    15961 agtttttcag gatcttcacc cagatcattt aattgttttt ttaagttttc ttctaaattt
    16021 tcttcatgta cccattgttc atacacttgt tcccaagaca tgaaaataac acctctttct
    16081 gaaatatggt tcttaataag tatagcattc acttattaga tatgaaagta tgaggaccaa
    16141 aaaacgctta aatcaagaaa ggattctttt atcaagcatg attgcaagta ttcacataaa
    16201 tcggattcag tggatttcac gcgaaaaaaa agagtaaaag aacaattgaa accgttcttt
    16261 cactcttttt aaacttatgc tgttgtttct tctaaagact ccaagtattt atacacttgt
    16321 tgtccagcaa taccgccgtc accaacagct gtagtgattt gtcgcaaatc tttttcacga
    16381 acatctccga tggcaaaaac gcctggcata ttcgttttca tttcgttgtc tgttggaatc
    16441 cagccagctt cgttcgtcaa acctgcttct ctaaaaggtg cagttaatgg atccagtcca
    16501 acatagatga atactccgtc tgctgctaaa tcaaattcat tgccggtttt gacattacgt
    16561 gctttcacac ctgtcactac catttcgttt ccaacgattt cgtcaacgac tgtatcccac
    16621 ataaaggaaa ttttttcatt tgcaaatgca cggtcttgga tgatcttttg tgcacgtaat
    16681 tcatctctac ggtggacgat cactacttca gaagcaaatt gtgtcaaata gatggcttct
    16741 tctactgctg agtccccacc cccgatgact actagacgtt tgttacggaa aaacgcacca
    16801 tcacagactg cacagtaaga aacgcctcgt ccagcaaatt cttcctcgcc aggtacgcct
    16861 aatttacggt ggatacagcc tgttgcgata atgactgttt ttccttcgta acttttatct
    16921 tcacagatga cttctttatg gctgccgtga tccttgatat cttgaacgat tccataagca
    16981 ttttctgtcc caaatcttga gacactttca tacatcttat aagctagatc ggggcccatg
    17041 atcgaatcga atccaggata attttcgatt tctgccgtat tgttcatttg gccgccaggg
    17101 gcaccttgtt cgatcatcaa tacggataag tttgaacgtg aagcatacaa agcagcagtc
    17161 atcccagcag gacctgcgcc aataacgatt acatcataca tatgtagatt ctcctttact
    17221 taatcaagta agcatacttt acagccaatg cgaaaaaaag tctattgttc tgcttgcgtt
    17281 tctcgaatag ctttattata ctcgaaaaat ccgctgaaaa acaatagact tttataaatc
    17341 attgatcaat tgttcgaaag attttctcca tttgaatcaa ttactttttt ataccagttg
    17401 aatgattttt tctttgaacg ggctaatgtg ccatttcctt ggttatcacg atcgacataa
    17461 atgaatccgt accttttctt catctcaccg gtaccagctg atactaaatc gatacatccc
    17521 caagtcgtat agcctaatag atcgactccg tctagttcta ctgcatcctt catcgcctgg
    17581 atatgcgctg ccaggtaatc gattcggtaa tcatcttcca cataaccatt ttcatctggc
    17641 gtatcgactg cacctaagcc gttttctaca ataaataatg gtttttgata acgatcataa
    17701 atatcattca tcgttattct caagcctaat ggatcaattt gccaccccca ttcacttgct
    17761 tgcagatatg gatttttgac agaagcaaag atatttcctg cagtctgttc caagagttcg
    17821 ttgtcggtag tcgaaactcg tgaagaataa taagaaaaag agataaaatc aacagtatgt
    17881 tctttcagca atgcggcatc ccctggttcc atttcgatct gaatgccatt tcgctccatt
    17941 tctttcaatg cataagcggg atattctcct cgtgactgga catcaatgaa gaaataattt
    18001 tctcgatctg attttctaga agcccaaaca tcttcaggat tgcaagtgta tggataatat
    18061 gctcctgctg ccagcataca gcctactttg ttctccggat ctacttcatg agcaatcttc
    18121 gttgcgatgg cacttgcaac caactcatga tgtgccgctt gatatttgat ttgttcttca
    18181 ttttctcctt cttcaaaata cagccctgcg cccataaatg gtgcatgaag gatcatattg
    18241 atctcattga atgtcagcca atatttgaca agtcctttat agcgattgaa gatgactcga
    18301 cacagatttt cataaaaacc aacgagttct ctggaacgcc acgcaccata ttctttgatc
    18361 aaatgcattg gacaatcaaa atgcgtaatc gtcacaagtg gctcaattcc gtgcttttgg
    18421 cattctttaa agacatcctc ataaaatttc aagccagctt cattcggctc tttctcatcc
    18481 cccattggaa aaatacgact ccaggcgata gacattcgat aagtcttgaa ccccatttct
    18541 gcaaacaaag caatatcttc tttataacga tgatacatat cgattgcaat ttttgctgga
    18601 taaaaatgat cctcatcaaa ttcaaacatt tttttctttc cggtgataac aggaaaacgg
    18661 tcttttccga ttggcacaac atctacgttt gctagtccgc gtccaccttc atcataagcg
    18721 ccctcacatt gattggctgc tgtagcaccg ccccataaaa aatcttttct gaaccccatt
    18781 atacttcctc cattatatgt gtattacctg ataattctct tgccagttca tcttgaactt
    18841 cgattccttc ttcttttaat cggctgattg tttccttgaa ttgcggtaaa tactgctggt
    18901 gtgcaataaa caactcatcc atgatccgtt ttgctgtctt tcctgatcgt ataagtggat
    18961 tgatcgtaaa cgcctgtaaa gctgtcccat aatcaccagt cacagctgct tcgatcgtca
    19021 gcaattccat attcttcatt acttgcaacc agcctctttc agcagcagga agagaaccaa
    19081 acgctacatt tctcgctcct tgtgcgccaa cataggcagt gacttctaca acacaatctg
    19141 ccgacaggtc aggaacagct ccatcattct ttgtagatac aacgatctgt gtttctttat
    19201 ttccatagat tgaagcaatc gtttcacagg cagcgtctga ataataagcg ccacctcgtt
    19261 tttgcaattg ttcaggttta tggtctaatt ctggatcttt gtataattca aatagctctg
    19321 cttctgtttc tttcacttgc tgcgctcttg taccaatcgt ttgatattct tctaaagcat
    19381 gttccagcat ttcttcttca cgatagtaat agcggtggta gccacaaggg atcatgttca
    19441 tttgttttaa ttgttctttg aaaaacggga tatcaaagat atttttcggt aatccattat
    19501 cttcatcgta taaacgatca atcaattctg ccgtgcggtc atttccttta ttatctgcta
    19561 ctttgtgcca atggaaatga tttagaccgg caaacttgta gatcagctct tcttttttct
    19621 tgccaagcat tgctggttct gtcatcatcg ccatcactgg cacattgcat aatccaatca
    19681 ctttatccca tttgccatat ctgataactg cttctgtcac catcccactt gggttcgcga
    19741 aattgatcag ccaagcattt ggacataaac gcttcatgtc ctcaacgatt gccaaaacga
    19801 ctggaacggt acgaaaagct ttgaatatcc cgccagcacc atttgtttct tgcccaatca
    19861 tcccataata actagggatc cgctcgtctt tgattcgtgc atctaattga ccgacacgaa
    19921 actgagtcgt aacaaaatcc gcatctttca atgcttcttc ccgatccaga gttgcataga
    19981 tcttgacttc atatggagaa gcatcccaca tacgctgaga catctgacta acgatctcaa
    20041 gcttttcttt tccttcttct acatctacga gccagatttc tcgtatcggt aattcctcat
    20101 atcttttgat aaatccttcc atcaactcag gggtgtaact gctgcctcca ccaatcgtag
    20161 cgattttgat tccttttgcc atgttctcac tcctttactt ttttacactt tcattatctt
    20221 ttttgtaaac aaaacaaaca atagaaatcc gtttctttcc agtaacattt acaaaagtta
    20281 ctgttttcta gtggattttt gtaaatatct tgtcaaaaaa agcctatttt aagttatata
    20341 gtataaatcc gtttgagagt gtaaaatatt ttgtgtaaat agaaaaaagg aagtcccttc
    20401 tgtagaatag agttaccaca acacattcac agaaaagagg acttccatat gaacgatttt
    20461 actacagaaa ttctaaagac tctagcgaac aaaggcgatt tgaatgaatt attccgtgtc
    20521 catttggaaa aagctgtcaa tacgcttctc aaaacggagt taacggcttt cctcgattac
    20581 gaaaagtacg atcgtattgg ttttaacacg ggtaattctc gtaacggctc ctatgaccgt
    20641 acggttaaga ccgagtacgg ggaacttcat ctccagattc cgcgcgaccg caacggcgag
    20701 ttcaagcaac agactgttcc tgcttataga cggacgaatg ataccttaga ggagaccgtc
    20761 attcacctct tccgaaaagg tattaccatg tcggaaatcg cagacttgat tgagaaaatg
    20821 tatgggcatc actacacgcc ccaaaccatg tccaacatga ctaaagtgct gactgaagaa
    20881 gtaaatgcat ttaaagttag aactctaaat gataagtatg tcgctatttt catggatgct
    20941 acttatattc ctttaaaacg gcaaaccgtc tccaaagaag caatctatat tgccattggt
    21001 atacgagaag acggcgctaa agaagtacta agttatgcga ttgctccgac tgaatcgacc
    21061 tacgtttgga atgagttgtt acaggacatt tactccagag gggttcatga tgtcttactg
    21121 tttattaccg atggcttaaa aggcatgaaa gatacgattc accagattta tcctaaagct
    21181 aaataccaac attgctgtgt tcacgtttcc cgtaatattg ctcataaagt acgtgtcaaa
    21241 gatcgaaaag aaatctgtga tgattttaag gctgtttatc aagctagctc aaaggaagag
    21301 gcaaatacct ttttagggag tatgattgag aagtggcaga aaacttatcc taaagtgacg
    21361 cagtcactga taaaaaatca agatttattg actttttatg agtttccgcc tggtatccgc
    21421 cgaagcattt actcaactaa tttaatcgaa tctttcaata aacaaatcaa gaaatacagc
    21481 cgcagaaaag agcagtttca aaatgaagaa tcactagagc gtttcctagt atccatcttt
    21541 gatacataca atcaaaaatt cttaaataga agccataaag gcttccaaca agtgacggat
    21601 acattagctt caatgtttac tgagtaaccc attattttgc agaaggacga gttatttaca
    21661 caaaattatt gacgctccct ttaagtgtaa tgcttttatg cacctattta atttatcatt
    21721 ctcatctgat acaatctcac tcataacaat atgaattttt cttctcatta gtctcagttc
    21781 accatgaatt atacataaca cccaatacaa ttatttttcc atac
//
LOCUS       DAXWLF010000031        21194 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXWLF010000031 DAXWLF010000000
VERSION     DAXWLF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.031"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(169..417)
                     /locus_tag="ACGE4U_000904"
     CDS             complement(169..417)
                     /locus_tag="ACGE4U_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393088.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            416..544
                     /locus_tag="ACGE4U_000905"
     CDS             416..544
                     /locus_tag="ACGE4U_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFF8393089.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            601..2295
                     /locus_tag="ACGE4U_000906"
     CDS             601..2295
                     /locus_tag="ACGE4U_000906"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFF8393090.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            2362..2691
                     /locus_tag="ACGE4U_000907"
     CDS             2362..2691
                     /locus_tag="ACGE4U_000907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393091.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFL
                     TWLDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLP
                     RGYEKGV"
     gene            3069..4202
                     /locus_tag="ACGE4U_000908"
     CDS             3069..4202
                     /locus_tag="ACGE4U_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFF8393092.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            4233..4553
                     /locus_tag="ACGE4U_000909"
     CDS             4233..4553
                     /locus_tag="ACGE4U_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFF8393093.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(4582..5046)
                     /locus_tag="ACGE4U_000910"
     CDS             complement(4582..5046)
                     /locus_tag="ACGE4U_000910"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8393094.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(5177..6430)
                     /locus_tag="ACGE4U_000911"
     CDS             complement(5177..6430)
                     /locus_tag="ACGE4U_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8393095.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(6459..6776)
                     /locus_tag="ACGE4U_000912"
     CDS             complement(6459..6776)
                     /locus_tag="ACGE4U_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8393096.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(6802..7122)
                     /locus_tag="ACGE4U_000913"
     CDS             complement(6802..7122)
                     /locus_tag="ACGE4U_000913"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8393097.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(7586..9001)
                     /locus_tag="ACGE4U_000914"
     CDS             complement(7586..9001)
                     /locus_tag="ACGE4U_000914"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8393098.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(9018..10334)
                     /locus_tag="ACGE4U_000915"
     CDS             complement(9018..10334)
                     /locus_tag="ACGE4U_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8393099.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(10484..11182)
                     /locus_tag="ACGE4U_000916"
     CDS             complement(10484..11182)
                     /locus_tag="ACGE4U_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFF8393100.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(11471..12464)
                     /locus_tag="ACGE4U_000917"
                     /pseudo
     CDS             complement(11471..12464)
                     /locus_tag="ACGE4U_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(12545..13378)
                     /gene="msrA"
                     /locus_tag="ACGE4U_000918"
     CDS             complement(12545..13378)
                     /gene="msrA"
                     /locus_tag="ACGE4U_000918"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8393101.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDTDYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(13612..14355)
                     /gene="truA"
                     /locus_tag="ACGE4U_000919"
     CDS             complement(13612..14355)
                     /gene="truA"
                     /locus_tag="ACGE4U_000919"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFF8393102.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(14546..15181)
                     /locus_tag="ACGE4U_000920"
     CDS             complement(14546..15181)
                     /locus_tag="ACGE4U_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFF8393103.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(15328..16548)
                     /gene="sstT"
                     /locus_tag="ACGE4U_000921"
     CDS             complement(15328..16548)
                     /gene="sstT"
                     /locus_tag="ACGE4U_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFF8393104.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            complement(16890..17567)
                     /locus_tag="ACGE4U_000922"
     CDS             complement(16890..17567)
                     /locus_tag="ACGE4U_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFF8393105.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(17818..18360)
                     /locus_tag="ACGE4U_000923"
     CDS             complement(17818..18360)
                     /locus_tag="ACGE4U_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFF8393106.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(18365..18592)
                     /locus_tag="ACGE4U_000924"
     CDS             complement(18365..18592)
                     /locus_tag="ACGE4U_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFF8393107.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(18671..20551)
                     /locus_tag="ACGE4U_000925"
     CDS             complement(18671..20551)
                     /locus_tag="ACGE4U_000925"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8393108.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            20837..21172
                     /locus_tag="ACGE4U_000926"
     CDS             20837..21172
                     /locus_tag="ACGE4U_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8393109.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
BASE COUNT         6521 a         4386 c         3590 g         6697 t
ORIGIN      
        1 tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt aaaagttgtt
       61 ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc tccctccatc
      121 cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt agtccgtcat
      181 cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc taaaaataga
      241 accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta caggaaaatc
      301 agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca tttggcattc
      361 taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt ttttcatgag
      421 taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg ctgcatatct
      481 aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat tcgaatcaat
      541 ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg aggaatcaca
      601 atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc agcggctgtt
      661 tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc attggatgat
      721 cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg gaataaaaac
      781 catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct taattaccgt
      841 acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga gccagggatg
      901 actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga caaagcacgt
      961 ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa taataaagac
     1021 cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa actagacaat
     1081 atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa cttctggttt
     1141 atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt gcgtcgctat
     1201 atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt caatcgaaca
     1261 cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa agaacaagga
     1321 tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac accgatgcaa
     1381 gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga agaagaatac
     1441 gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac agattcagct
     1501 actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc agcttctagt
     1561 ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa attctttgct
     1621 gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca tttgttgttg
     1681 aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc atttatttca
     1741 accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt cgtgcatcat
     1801 caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta tttcctttat
     1861 ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg aaaagaaatg
     1921 ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa tattcgtgaa
     1981 aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat gccttatgct
     2041 tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc taaacattcg
     2101 accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat ctttactgaa
     2161 caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat tcctatgtcc
     2221 cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg tgccgcaaaa
     2281 aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     2341 accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc gcagatgcaa
     2401 gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca agtaataaga
     2461 acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt accgcagatg
     2521 caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca tcaagtaata
     2581 agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt attaccgcag
     2641 atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta gtttcgagta
     2701 tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga cttaataccg
     2761 agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt tttgaaaaca
     2821 actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga tacaactatt
     2881 tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac aaatacataa
     2941 gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg taagatctcc
     3001 tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat ctgaaaggag
     3061 catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc agaaacgtag
     3121 atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca acagccattt
     3181 gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa gggtatgttg
     3241 taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa acattgaaaa
     3301 aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat ttctgcctga
     3361 atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc gggcttttcg
     3421 gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta agagttcctt
     3481 atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa aaagttttat
     3541 ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga gtaaaaaaag
     3601 gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa aaattcgctg
     3661 ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt ctagataaag
     3721 cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa gctggaacag
     3781 aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta ggaatggacg
     3841 gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc ggcacaatct
     3901 ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa gtaactggtg
     3961 tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg gatatcgctg
     4021 tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca atgattgcag
     4081 aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca gaagcagcac
     4141 aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt ttgaaacctt
     4201 gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga aatacccatg
     4261 agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc cgcttctatt
     4321 tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg attttctata
     4381 tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc tatttttatt
     4441 tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg aacattttta
     4501 tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca tgagaatttt
     4561 tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac gagcggtcgt
     4621 cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat aaaacgtggt
     4681 atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt ttctgatttc
     4741 cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa acagggcaca
     4801 aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc ccgctcctcc
     4861 gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt ccttattagc
     4921 gattccagta cgaatacttt cctcatacac aacagcagga actccccatt ttgatgaaaa
     4981 ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc agttatatgt
     5041 aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac aagtctttat
     5101 gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa tcacttgttc
     5161 tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg atcgtagcta
     5221 ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg gctagtacac
     5281 cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt acccatggag
     5341 gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca acactaacta
     5401 agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg ataggtaacc
     5461 caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag tttgttacaa
     5521 ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa cctaaagttg
     5581 cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg ataaacggtg
     5641 cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca ttgattgttt
     5701 gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag aatggtgtga
     5761 taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat ggttcttgga
     5821 ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa attgcagcaa
     5881 ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat gcttttgcaa
     5941 ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg cttaaacgta
     6001 caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg tttgagctca
     6061 tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct gcggaaacac
     6121 cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc aagacagaaa
     6181 gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat tcaccaccaa
     6241 aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc attactgcta
     6301 atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc gcaactaaat
     6361 gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata aatgcaacaa
     6421 atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa tcgacgatac
     6481 agatcaatga tctcttttgc taaatctgta aaagctatag atgtcatcag atgatcttga
     6541 ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg tgtcagcatc
     6601 tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat tttttcatct
     6661 gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc gctttttgca
     6721 tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc ttccatttat
     6781 tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt cgagtacttt
     6841 ttcaccattc atcatgccat agtcagccat attgattaca tctaaaggaa tgcctttagg
     6901 tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta aaagtaaaac
     6961 gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag caaagatgtc
     7021 tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga gacttgtact
     7081 cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca cgttcctttc
     7141 tatacttaca cttgtatata agcaagtagc gtgccaactt taaaagcgct ttaaaaagtt
     7201 gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag aaagcgcaca
     7261 ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa aaaaagttgt
     7321 gacacaaaac ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt gcatctgcgg
     7381 taataagtcc aaccaagtta aaaacataag atgttgtttt tactttgcta ttcttattac
     7441 ttgatgcagg acagcttttt cacaacctct caataaacgg tgttcaatca gctgaccctg
     7501 ggtattagct caaaacgctg gattcacaac ctcttttatt tcaaataacc agtaaataca
     7561 atgaacatct acaaaaaact tctatttatt tatagatttc gtcaggcaaa tccaattctc
     7621 tcttatcaat caaagtctga taccacgaag cagatttttt caatgttctg cttcgttcat
     7681 cgttcaattc gattcgaact aatccatatc gattcttaaa tgcattcatt ggtgacacac
     7741 agtcagtgaa tgcccataac atatagcctt cgcaatttgc gccttcttct gttgctttga
     7801 tcaagtaatg caaatgctgt ccgataaatt cgatacggta atcatcttgc acgattcctt
     7861 tctcatccat atactgttct tcatattctc ttcccatacc gttttctgtt atcagccaag
     7921 ggatattatg ataattttct tttagataca tggctatatc atacatgatt ttcggataga
     7981 tttcccatcc cctggaatta ttcattttct tcccaggtaa atcaaagact tcataatatc
     8041 gttcaggatg gaaaggtgtc tcttcattcc attgataacg aggggcgcga acgcgtttag
     8101 gataatattg attgattcca aggaaatcta ctgtatgatg ctgaatcgtt tctaaatcct
     8161 ctgttgcagg atcgaaataa atcgattgtt tttgacacaa ctccaggaac tcttctgtgt
     8221 agtatccttt taccatcgga tcaaaaaata ctcgattata aaacaaatca tacatataag
     8281 cagctttttg gtcttctgga gaattggatc gagcatagac catttcagga tttaaaatac
     8341 agccaatcct tccaccataa tttccgctgc ggtaaatctc tactgcttta gccgttgcta
     8401 aaactttatg ataattccac atcatccact tttttgtgtt ttgttcatgc ggccagcgaa
     8461 cagcatccaa atagcaacgc gtttggggaa cgattggttc gttgaatgta aaccagtttt
     8521 ttaccctatc accgtaacga tcaaaggcaa tttgagcata cttagcataa aaatcaacta
     8581 cttttcttga agaccagcca tcatattttt ccaaaagaaa agctgggact tcataatgtt
     8641 ccaaacaaat catcggttct acgtctgctt cgatcaaaga gtcgatcatt tcacttatat
     8701 gctgagcata ttcttcatct actatacagt tctcataatc agtaaaaaaa cgagcccaat
     8761 tgatagaagt ccgataatgt gtcagatgga tggatttcat cagatccaca tcctgctggt
     8821 attgttccat aaaattagtt gctacggctg ggccataccc attatgccaa acaaaatgtt
     8881 catttttata ccagctatct ataaacgaat cctgtccttc tttttttcct ttccaacctt
     8941 ccgtttgcca agcagaagat gcagcaccaa gaataaaatt ttttggaata gtaattttca
     9001 tgatttcctc ctgtttttta gatcccatca ttttccacac gatttgctac ttttacaaat
     9061 ggcagataga tcaaaataga aacaataata caaacaagtt gcgtaataac tgcaccgata
     9121 tttcctgctg ttgccagata agcattgacg atcggcggca ttggccaagg gaccattaca
     9181 actgctttcg ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc agtgataaga
     9241 ggcgttaaga taaacggaat agctaaaatt ggattcagca tgatcggcat accaaaaata
     9301 actggttcat tgatgttgaa gatcccagga gcaagggata acttggtgat ttgtttcata
     9361 tcttcccgtt ttccaactaa caatactgca atcagtaaac cgatcgtgac tccagacccg
     9421 cccatactca tatacatatc ccaaaatggc atagtaataa tgttcggtac ttcttttcca
     9481 gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa gcaacggttc ccgtatcggc
     9541 ttcaccattt gatttccatg gataccaatc acccaaaaga cttgcgcaat aaacatcaat
     9601 agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac gacattgtaa
     9661 atgatatcat aaaggtacat tcctgtaagt tctttgatga taaatcccgc agccgcgata
     9721 cccgatacag tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac attcgttggc
     9781 acggtatcag gcattctcag ctgtagtttc ttctgtttcc tcaaccagca ataaagttca
     9841 atagaaagga tggagaccaa cattcccaaa aataaccctt tcgtatctgt atattgttta
     9901 gccaatacgt tttctactat aacagtctga ttttcattca ctaattcata aacagttgga
     9961 ttaacagata aataagacat cactgcgatg ataccaggga atacaccatc cactttattc
    10021 agcttggcta attcgatccc tatcagaaaa acagcataga tggtcaagaa acttagtgtg
    10081 acatagctga tggcagatgc gataggcttt aattcttcta aaaaacgcaa gaaacttatc
    10141 tgtgctaagc cattttccga gtcaaagacc attgctgaaa acaatgtgcc aaaagcaccc
    10201 gtaataatta ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat ataccttaaa
    10261 ttattgattc tcgttgccac gttccctaaa acattggcta atttatccat aaaactcatt
    10321 ccagatcccc tcattcctgc ttcttctcct tgacgtcatc tctttttgtc tattagtcag
    10381 cgcttacatt tcgatgaaca gaaactattt actcctcttt tttaccaaat ataaaacaaa
    10441 aattcatctg atgtatttat cataaaatac atcagatgaa ctgtcaatta gtttcttttt
    10501 ctcctattat tttctgtaat tctctccgat ttattgccat atggacagtc attgcatcaa
    10561 acgcagcaag tgcattccgc ctttcgatgg cctgaacgat ctctgtatga atatcgatcg
    10621 tctcgatttt cagatgctgg atatcataat ttttattaaa caaagcaatt gattcattaa
    10681 tgattgggat aatatgcagc aaggctacgt ttccgctagc ttctgcaatc atcgtatgaa
    10741 aatcgatgtc taattgaaca tgttcttgtg tgccttctcg aaaagaagtt tcaatctttt
    10801 gcgttaattt tttcatttgg acgatttgat tcttcgttgc atattccgct gccaaacctg
    10861 ccatcttagg ctccaataaa tatctcagtt caaataagtc tcgtatcatc cgatcctgat
    10921 cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc tcgcttatca ctgacaaatg
    10981 tccccgctcc ttgccgaact tcaaggatat tcctggaagc tagagcccgc acagcttctc
    11041 gcaccgtgct tcgccccacc tctaaataat cggacaattc atattcattc ggcagtttat
    11101 ctcctacttg caactgcatt tcctttaaat atctaaggat cttgtcagta gttacttcta
    11161 ctagggtttt attttcttcc aaaccacata cctccttttc atctgatgta ttttttcgta
    11221 attattataa cacgtttgaa tcatctgatg attttttgta tacaaaattg attaatccgt
    11281 tcatagacat acaaacgaag aagatattta ccataaaaaa gactaggata aaattcactc
    11341 ctagccttcg atcattttta ttttgaacga caaaaaatat gatgttcaat aacagacgat
    11401 cttcattcga ttcttgatgt catcttttgt tttttttaat atatggataa aaagtttgat
    11461 tacatcgttt ctattctaga atttggtcga ttctcaattc ttcttctggt gagaaatcta
    11521 aattatccat cattcgaaca ctttcttgta actgacttaa ccgacttgct ccgaccagta
    11581 cacttgtaac agatttttgt cgcaaattcc atgcaagggc catttgcgct aatgattgtc
    11641 ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc cagagttgat ccaacttgtt
    11701 cttctgaaag gaatggtatt tccttacgat gtgctctaga atcttcagga ataccatgta
    11761 gataacgatt tgtcagcaac ccttgagcaa gcggactgaa cgtgattgcc cctagccctt
    11821 cttcttctaa tactggagat agatcgtctt ctagtagtgc acgagaaaac atattgtagc
    11881 gcatctgatg aataatgaac ggtgcttcct ttctttttaa aatttcagtc atttttttgt
    11941 atcttctcca ttatagttgg atataccaat atataatgct tttccttgtc ttacaagttg
    12001 catcaacgct tctgctgttt cttccaaagg tgtatcaggg tctgggcgat gatgatagaa
    12061 gatgtcaaca taatctagcc ccattctttg gaaactttgg tcacaacttg atatgatgga
    12121 tttacgagag ccccattccc catatggccc ttcccacata tgataacctg ctttgctaga
    12181 aataatcagt tcatcacggt aggcttgaaa atcttctcta aaaatacgcc caaagttttt
    12241 ctctgcagct ccagcagggc ctccataatt attcgcaagg tcgaaatggg taattcccat
    12301 gtcgaatgcg cctcttaaaa tctctctttg attatggata ggatcataat caccaaaatt
    12361 ttgccataat ccgagaggag agataaaatc ggtaatttta aacctgaatg accggattta
    12421 cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc tcctcctcat
    12481 caaatctttt taaaacgctt tcttgttttc tatttttagt ttactacatt ttaaagaaaa
    12541 ggagttaatc aaaatgattt tttcgtatat ttttttgcca tttgtttttt actcgttgat
    12601 agatcagaaa ttgattacgt gcttgctgga tttttttata gcgtttcggt tgttttttgt
    12661 aaaattgctg atgatagttt tctgctggcc aaaacgtacc ggctggttcg atttttgtga
    12721 cgattggttg gtggaaagtt cctgaactgt ctagtttctg ttttgattgt tccgctaatt
    12781 ctttttgtcg ttcgtcttga taaaaaatca ctggacgata ttgcttccct ctatcttgaa
    12841 attgtccaaa tgtatctgtg ggatcagtaa tttgccaata gattgtcagc aactcgctat
    12901 aacttatttc tcttgtgtca taaatgatct ccacagcttc cacatgacct gtgtaaccac
    12961 cacttacttg atcataattc ggtttttcaa catgtccgcc cgtgtatcct gacaaaacag
    13021 ataggatccc ttttttcgat tcaaacggct cgaccataca ccagaaacat ccccctgcaa
    13081 atactgcacg ttcttgatag tcagtatctt gcatggcatg cttggaaaag tcatattttt
    13141 gctccttatc aaatccttcc gtaatcaata aataaaagtc cattacatca ggcgtcagat
    13201 tcattcggat agctaatgga cgtagtgtta cttctagttt tgataactgt tctttaacgg
    13261 atatattttc tcttgtgtca ttctttgtct gaatcaatac ttttcgttcc caatctcgtg
    13321 tattcggatt caaaatcaga ttataaagtg tttgaatgaa tatttgttcc tcatccatag
    13381 attctcctcc tttccgaaga agacaagcta gtgactttag ctgtaattta tcataaagtg
    13441 aggaaataat cgattgaatc gtatttattc aaagaagata agcagcttca gatgatataa
    13501 gatgatcttt atcttaaaaa gcatactcta tggtttggtt taactaaaaa acgacctctg
    13561 aaaaaaggga tgttctccaa ttctcagaag tcgagtaaag atgattcttt tttatgagcg
    13621 ataataaact tcatccaaaa acagaccttg tgcagggacg gtttctcctg catgttgccg
    13681 cactttgctt tcaaatattt catcgattgt ttctatcggc atggcacctg tgccgatttc
    13741 tagtaatgtc cccatgataa tccgcaccat tttatataag aatccgtctc cgacaaaagt
    13801 aaaatgaagc atttctccct ttcgttcgat agataattca tcaatcgtac gcactgtgga
    13861 tttttttgat tttttcaatg cagaaaaacc aatgaaatca tgtgtaccca ctaatttttc
    13921 gcaagctgca ttcagcttgt ccatatccaa ctcttgaggg aaataaaagc tgtggtttcg
    13981 ctcaaatgct gaaggaatca cgttgttcca aacataatag ctgtattttt tgcccaccgc
    14041 attatatctt gaatggaagc gctcaggcat ttcttcgatt cttttgatga caatatcccg
    14101 cggaaggtaa cgattcataa agtcgatcat ttctgccagt ccaatttctg tgtttgtttt
    14161 aaaatttgct acttgtcctc gcgcatgggt gccagcatct gttcttcctg atccaataat
    14221 ttctatctct tcatttgtca tctgatgcaa tactttttcg atttttcctt gaatggtttt
    14281 atcgtcatct ccaagtcttt gccatcctga atatcttttt ccgtcatatt cgattgtcaa
    14341 cttgatgttt ctcatatttt agtggctcct ctgtcttcca tttggtcttc tctctacctt
    14401 tttaagattg tagcacaaaa tatagctcct tgcttctcta agacagaagc tatacaagaa
    14461 aactgtggaa atatatattt cagaaaaaca aaaaacagcc accggatgaa gtatccgctg
    14521 actgttcaat ttgacggctc aagttttact gtttgagatg caagatactc aaccaggcaa
    14581 tgggcacata attaaagaaa aaactgattc tttggcaaaa acctatttta ttcagtaatg
    14641 gccaagaatt tatagaagga attcttgcca gtccataaat cgccgcaaaa agcaaactga
    14701 tcacagacag tccaaggtat aagtggctta cgtccatttg cccatttttt cgatagatca
    14761 agaaaagaag aactggaaac aataaaaaac cactgtaacc taaacctgat ccgatattat
    14821 ggacccatgt cgagacattc cacgaagatt catgggtatt gatactgaac aaacctgtaa
    14881 agatacaatc acctatacca tagaacgcga tggctgctgt aactagggta gctaacacac
    14941 gagatgtact tacaaatgca gcatacaatg caggcaatga caggatgaaa aagatccctg
    15001 aaaccacaga ccaaataaga aacgcttttc ttacaggact ggaaacctct ccaaatacac
    15061 tgatcacgct cttcatttga tctaattcag gataatacaa tcctaatacg tacggagtca
    15121 aaaaatcact cagcacgcct aataataaaa agaaaaaacc aaatttacgt aaaaaatcca
    15181 tgatacgcct ccaataacaa ttacctatgc ttttaattgt tattataaac gactttatct
    15241 tgatttaaca gaaacctcta tttacacgaa catctatata actcattaaa aactcgaatc
    15301 attttaaaaa tactaaatat ctaacgttca acgttcactt aatccatcta aacaatcttt
    15361 caatttgatt ttttccccac ttcttttacg cgcgcctagc tctcctattg cagtaaacaa
    15421 cagatcactt gacgaattta aggcagtttc cattgaatcc tggataacac cgataataaa
    15481 tccaatacca acgatttgca tcgcgacatc attagagata ccaaacaaac tacatgctaa
    15541 tggaatcaat aacagtgaac cgcccgcaac acctgaagct ccacaagcag ctaaagcaga
    15601 caaaatacat aaaaggactt tcaaaacaaa tggtgcttca atccctaaag aatgtacagc
    15661 agtcaacgtc atcaaggaaa tggtaatcgc ggcaccgccc atattgatcg tagcacctaa
    15721 cgggatcgat atggaataag actcttttgt caatttcaat ctttcagcca acatcatgtt
    15781 tatcgggata tttgcggcag aacttcgggt aaagaaagcg ggaagtgcgc tttctttcaa
    15841 acaaaagaaa atcaatggat agggattttc tttaataaac aaaaacgtga ggaatgggta
    15901 cacaactaat gcgacaaaca gcatcgtacc gatcaaaaca agtaaaagct gcagatactc
    15961 tgccaatcca gcgattccag tcgtagcaat tgattgataa accaatccta aaatccctat
    16021 cggagcaaat gcaataatca cttgtacgac ttgtgacaac gctatagata tctgatccac
    16081 caactctttt gtcggttctg aacgtgctcg aaaagctagt cccaacaata cagcccagaa
    16141 taaaacgcca aggtaattcg catcaataag agattgaaca ggattttgtg tgacattcgt
    16201 caacatagta gataaaacac tttccaaact ttgcggtgct ttttcagata ctgtttcttg
    16261 taaagtaata ggaacagtaa aaattgaact tacaatgact gctacaagtg cagaaagcaa
    16321 tgttcccact aaatataaga tcaaaatagg cttcacgaat gtcttattcc caattttatg
    16381 cttggcaatt gacgccatcg tcaagaaaaa aaccagaata ggtgcgattg ccttcaagca
    16441 actgacaaac aacgtcccca atattgaaat aaatgtccac gtggggaaaa agataccaaa
    16501 caatattccg actaaaatac caacaacaat acgttgaact aacgacatat ccaaaaactt
    16561 ctctaacaaa tgaccaccct ttcacaattt taacaatcat tgtatcattt tatctaaaga
    16621 aactaaaggt gcaattttct aaaaattcta ataatacaat tggcaattca tgctcttgca
    16681 acattcaatt ttagtattgg agggtttatt caatctctaa agaagtcgag agaaaactat
    16741 ttatcgttat tattatagtt atccaagtaa aacgtaacta agatgaagga cgaaactaga
    16801 aatttcatcc ttcatcttaa ttcttagaca tagcaatcaa ttaatatttt ccactgagtc
    16861 tcttctttct ctcactctca ctggtttact tacccataaa cgatttgatt aaagccattt
    16921 ttcaattcat tattcgctgt ctcgttaaag taaagataat tatcgggatt cccctgattt
    16981 tgttgtgtca ccaatacttt tggctgattt tgctgaaacc cgaatagata gacgtgcttt
    17041 ttcagatatg gttctgtctc tgcatcggaa tagacggctg tgatttgata gtctgcttga
    17101 cctgtcccac tttctgacca ttctgcagaa acaggcactc caccaatagc catcttccac
    17161 tccccgttca aaataacttg agggacttta agaccatata agtccacact catttgattc
    17221 gtatagcttt tgtattgttg tcccattgtt tggccccatg tagccataaa tgcctctaat
    17281 tgtgcggatt tgtctgcatt ccacagtgtt tgttcgactt gttcgctgct ttctgaagat
    17341 gaagcctcta cagaagaact tgaagtagat gtttgagttg tttcagtcgt tttggaagac
    17401 tctgacgaag tagaggtatc tgctgtttcc gtacttgctg cagttggctt gctctctgtg
    17461 ctagttgttg tagtagttgc aattgttgct ttcgctgttt cttttgttga ttcttttttc
    17521 gattcattcc cgttggtaca accagctaac aaaaaagcta gaccaattgt tactaaaatc
    17581 ctttttttaa attaaaaaac tcctttcgat tactcccact tctttattct atcaaataag
    17641 caatgtgatt ttattgattt tttgtatttt ctcatccatc ttttaaacct tttaaaaact
    17701 aagaagcacc cgctgatgtt gtctgtaata catcagcggg tgcttctttt attttaatct
    17761 tcgcgatttt ccaatttacc tcacgcttta tatttgtatt tgaagaaaca tagactttta
    17821 gtcatcatcg tcttcctcat ccaaatcttg tcggacagtt tttccttgga gtctttgtac
    17881 ttggtagatg atttctttat tgaaacttaa aagttcaaca agcaattgtt tcaagccata
    17941 tttttcttct ttttcagcta atttgattgc ccgtgtgata aagacatttt ggaaatcaaa
    18001 atcttttgca aagttttcca acattgttga ggctggtaaa tatttgtttt gaccatgttc
    18061 gtaaattttt ccataacgca tgacttcttc taaagtagac ggaacgattt cattttcaat
    18121 cactaactca tgagagattt tattgaacca tctcgcttct tgatcagcat attctgaaaa
    18181 aagggtactt aattcttgcg aagatgtacc caaaacatac aattgtgctt ggatcaattt
    18241 catttcttct aatttaaaat ttgccaatac atgccccacc atggcgccag cagttggttt
    18301 atgatgatcg atatctgctt gttttgtttc ttgagcaaat ttttcttcca ctatattcac
    18361 tgatttacgc ctcttttact tttgttgatt ggacttcata acctaatttt tccacaactg
    18421 cagctaattg ttcagggtgg acttcatctg gatcaaagtt tgctttgatt ttgctcgcat
    18481 taaacaaaac tttcgtattg cttaccccag gctgttgaga aaccgcatgt tctatttttt
    18541 gcatacagct tggacaagct agtgtatcta attgcataat catttttttc atcgtagttc
    18601 ttccttcctt tttcttgata gtcttattct agattacagc ttccataaaa aaattgattc
    18661 ttatcaagtt ttacgatttt tcttctttgc tttcgtgtcc aaaatacaat aaacgcatcg
    18721 cgttaaagat aacgatcaag atactagctt cgtgaacgaa catcccacta gccatgtaaa
    18781 taaacccggc aaataagccg atgagtaaaa ataagacgac accaatggca atcactacat
    18841 tttcacgggt attcaaaaca gttttcttag ccaaacggta agcatgaacc aacgatccaa
    18901 aactagattg catcaagaca acgtcactcg tttcgatagc gacatccgtt cctgatccca
    18961 tagcaatccc gatatcagcc gcagccaaag agggagaatc attgattcca tctccaatga
    19021 aagcaacgtg caatccttgt tcttggtatt ttttcacaaa ttgaaccttt tcatctggca
    19081 ataattcagc atggacttca tcaatgccta acatgtcagc tacataatca gcagtcatct
    19141 gattatcacc tgtcagcatc actaaatgtt ttgctcctgc ttgtttcaac atagctaatt
    19201 gttcagcgac ttctggtcga atcacatctg acacaccaat gatttgagtg atttttttgt
    19261 cgattgctac aataatgact gagctgcctc tttgttgtaa ttgatgtaaa tcttgtactt
    19321 gctttggtgt gagagaaagt ttgtgagcgg caatcaattt ggtgttgccc gcacaaactg
    19381 tatgaccatc aatctcagct gtgattcctt gtcccttgat cgtctgatta tctttgactg
    19441 ctaattgttg aaaatcaatg tctttctcct gagcataatg gatcacggcg cgccccagag
    19501 gatgatcgga taacgtttct aatcgagcca ctaaagctaa gctgtcccct tcatcctctg
    19561 tatagttctt gatagtggat acagcagtag ttcctttggt caatgtaccg gttttatcaa
    19621 agacaaaagt atcgattttg gaaaaagtat ccattgcttc accgcctttg actaagatgc
    19681 cccgtttcgc tccattaccg attcctgcta cattcgaaac aggcgcacca atgactaaag
    19741 cgccaggaca accaagaacc aaaacagtga tggctaaacg aaaatcacga ctgatcagac
    19801 caacaagcaa cgcaataagt aaaacggttg gagtataata ctgagaaaat tgatcaatga
    19861 atttttccgc atgtgatttg gtatcttgtg cttcttcgac taattctata attttggcaa
    19921 aggtcgtatc atctccgacc ttagttgctt cgatcgtcac gtagccatct gctaaaatcg
    19981 ttccagaaaa tacgttatct ccactgattt tgacgacttg tcgtgattca cccgtcactg
    20041 cggcttcatc cgcataccct ttcccgtcaa tgactcgtcc gtccacagga atagctccac
    20101 cagtttttac taagacgata tcgccttctt ccacgtcatc gatttccact tcttcggtcg
    20161 ttccatcttc ttggacaacg ctggcagtcg atggggccat ctcggtcaaa gccttgattg
    20221 attcccgcgt tttggtcaat gttttttgtt ccagataact accaaacaaa aacaagaatg
    20281 taacgatagc tgactccttg tattctccga tcataaaggc accaagcaca gcaatagtaa
    20341 cgagtaaatc aattgagata actttgactt ttactgcctg atacgcatgg attataatag
    20401 gtatgaccgc aataatagaa gctatggcta gagcaatgtt atagaacaag acaaatccag
    20461 taaatttccc gataaaacca aaaataatca acacagctga taaggcggtg atatgatttc
    20521 gatgctcttg gatccacttt tgtaatttca tctttctccc tcttttcctt ttcattctta
    20581 cgagactaag tatagatact tatccatcat aaaaaattga ccactatcaa gtttattcaa
    20641 aaaggcggtt ttcttttggt atactgaatt cctacagtca ttaaacgatc atatagaaaa
    20701 aggaagcgcc gaaacgcttc cttaggtagt tctatttttt tattcaaaga taagatgctt
    20761 tgtcatctta cgactgaaga acaacaatca aaaaagggca gcagccactc attcacaaga
    20821 aatatcgttt tttgatatgg gagttgaagc agtggacaaa tatctctact tattagctgg
    20881 gaataagatc caaaaaagct taatggattt tatccaagaa ttggaatgta ctttccacaa
    20941 aaaattcact cattccattc ttttaaaact tttgattcat acagcgtgtc taattgagcg
    21001 tacattgata aacggtcatg aacttaaaat tatatcagaa gatgacacaa gaccttctca
    21061 tgaaacaatt tttcatgtaa aaaaggcctt caaaaatatt gaaacagaat ttgggatcac
    21121 tgtttcttat gatgaatgtt tctttatcta tgatattatc gcatctaaat aatctttttt
    21181 tatgtaaacg agta
//
LOCUS       DAXWLF010000032        20806 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXWLF010000032 DAXWLF010000000
VERSION     DAXWLF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.032"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(568..1593)
                     /locus_tag="ACGE4U_000927"
     CDS             complement(568..1593)
                     /locus_tag="ACGE4U_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8393110.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1868..3553
                     /locus_tag="ACGE4U_000928"
     CDS             1868..3553
                     /locus_tag="ACGE4U_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFF8393111.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTNKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3567..4850
                     /locus_tag="ACGE4U_000929"
     CDS             3567..4850
                     /locus_tag="ACGE4U_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8393112.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4920..5789
                     /locus_tag="ACGE4U_000930"
     CDS             4920..5789
                     /locus_tag="ACGE4U_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8393113.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5786..6622
                     /locus_tag="ACGE4U_000931"
     CDS             5786..6622
                     /locus_tag="ACGE4U_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8393114.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6648..7715
                     /locus_tag="ACGE4U_000932"
     CDS             6648..7715
                     /locus_tag="ACGE4U_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFF8393115.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7712..8539
                     /locus_tag="ACGE4U_000933"
     CDS             7712..8539
                     /locus_tag="ACGE4U_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFF8393116.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8584..9609
                     /locus_tag="ACGE4U_000934"
     CDS             8584..9609
                     /locus_tag="ACGE4U_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8393117.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9606..11789
                     /locus_tag="ACGE4U_000935"
     CDS             9606..11789
                     /locus_tag="ACGE4U_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFF8393118.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11789..12917
                     /locus_tag="ACGE4U_000936"
                     /pseudo
     CDS             11789..12917
                     /locus_tag="ACGE4U_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            12914..13591
                     /gene="pgmB"
                     /locus_tag="ACGE4U_000937"
     CDS             12914..13591
                     /gene="pgmB"
                     /locus_tag="ACGE4U_000937"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFF8393119.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13675..14202
                     /gene="msrA"
                     /locus_tag="ACGE4U_000938"
     CDS             13675..14202
                     /gene="msrA"
                     /locus_tag="ACGE4U_000938"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8393120.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14403..15089)
                     /locus_tag="ACGE4U_000939"
     CDS             complement(14403..15089)
                     /locus_tag="ACGE4U_000939"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFF8393121.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15169..16707
                     /locus_tag="ACGE4U_000940"
     CDS             15169..16707
                     /locus_tag="ACGE4U_000940"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFF8393122.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16827..17294
                     /locus_tag="ACGE4U_000941"
     CDS             16827..17294
                     /locus_tag="ACGE4U_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFF8393123.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17828..18256
                     /gene="rplM"
                     /locus_tag="ACGE4U_000942"
     CDS             17828..18256
                     /gene="rplM"
                     /locus_tag="ACGE4U_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFF8393124.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18272..18664
                     /gene="rpsI"
                     /locus_tag="ACGE4U_000943"
     CDS             18272..18664
                     /gene="rpsI"
                     /locus_tag="ACGE4U_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFF8393125.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18752..19978)
                     /locus_tag="ACGE4U_000944"
     CDS             complement(18752..19978)
                     /locus_tag="ACGE4U_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8393126.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTSDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20050..20352)
                     /locus_tag="ACGE4U_000945"
     CDS             complement(20050..20352)
                     /locus_tag="ACGE4U_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8393127.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
BASE COUNT         6712 a         3740 c         4323 g         6031 t
ORIGIN      
        1 ttattgtcac aacctctttt ttggtttgtc taaaataatt tgtctaacta gaagaaaagt
       61 gggaaaggat cttaacaaca tcggttcgta tccaattaag gtatcggaac gatcatcttc
      121 caagagaagt caatcaacaa aagtaaagat agatcatttt ttgaatgcaa aataaattga
      181 tgggaagata gagcggttat ttttaatatt ttgctagcta ttattgaata attcctcgaa
      241 taaaacaaaa ccagtagaca tgtctgttat tctatttatg ttttagagat aagtatcccc
      301 aaataacatg gattgaaata agggtaaatg tgtttaaaaa aacgaactat aaaaacttat
      361 acacaacgtg gttaaaaata gttttttctt ccctgtggaa aaaatagttt aacacaaaca
      421 ttatagttat ccacagctat ctaaaacaga tggaaaataa gaaaaattcg ctaaaatacc
      481 gggagggggt aacgagggat gagtgattga aaaaagaaca gagtaactga cttatcctca
      541 gtgtttctgt tcttttataa ttctgttcta catttcttgt tgttggatcg aatctgtgag
      601 agtaatctcg gttgaaagaa tgattctttt ttcttcaggg tattggtggt ggattagttg
      661 gaataaaagt tctgtggctt tgattcctaa ctgatttgtc ggaatgtcta tccgagtcaa
      721 ctgtggcgaa aatagggagg acaataaggg atcactgaat cctgcgatcg aaagatcatc
      781 aggtatctgc tttccagatt gttctgccac tttgtaaata ctcaatgatt ttaatgcatc
      841 ggtagaaaaa atcgcagttg gtctttctcg tagcttcatt aaatcattca ttgctgccat
      901 gctatcttct aagcgaaagc cgccgtctat aatccattca ttacggatat ctaggtggtg
      961 atcaaataag cattgacggt aaccattgag acgatcattg gatacataaa cttctgtcgg
     1021 agcatggaga cagccgatat gacggtgtcc attcttgatc agtgtttcca ccaaatcgta
     1081 actatcctgg aaattatccg tgtccactga gtagatgttc gtgtagtcgt ttccgacttt
     1141 tccgattacc acaataggaa tattgtattg atccaattca aaaagaagtg tttcatccat
     1201 cgtagagctt aaaataataa ttcctcggat cattttttct tggatctttt tcgcgcattt
     1261 ttctagctct tcttctggtg tttttgccgt ttgtagaatc aagtcgaagt tattttgttc
     1321 agaaacggca gaaatggatt gaataacatc tgaaaaaaaa ggattgccat aggtcgtatg
     1381 agaagtacga tgagataatg ctagaatcgc gtcaaaacca tttcctgaca gtcctcttgc
     1441 aattttgctc ggttcataat tcaattcttt gattgcttga agtacacttg cttttgcttt
     1501 atcagagata tttggctggt tatttaatac tctggagaca gttgattttg agacattagc
     1561 ttttttcgca atgtcataaa tagtaggttt caattttata tgcacctcct ttttgggagc
     1621 gttttagaat aattccatag tacaatagac attctttttt gtctatgatt ttgaaaattt
     1681 aggaatagct tgcatgatca tgagagacca tattttttta agaagctatt aattattgat
     1741 acataagctt tcttatttgt atttttgtgt atatcaaaat tatttaaaaa aatgctattg
     1801 acagtaagcg ttttccataa tatgatcaaa tcgttgggaa cgctcccaaa aaagaaaggg
     1861 gaatccgatg tatacaaaac ttttacaagc gatttatcgc gactcagatt tgtctgagaa
     1921 aaccgcctct atgattgaac aaaaagtcag tgtttatcag cagaagaaag gccaggcagg
     1981 caaactgcat tgggacgaag aagatatttt cttgattact tatggggatc aattttttga
     2041 agagcagcaa ccaactttgc gtacatttaa gaaattttat caaaagtatt tggcagatac
     2101 atttagagtt gtacatttct tgccattttt cccatataca tctgatgatg gtttctccgt
     2161 tgtaaattat caggcaatca acgaccgttt gggcacatgg gaagatatag aagaaatgag
     2221 aagcgaaaca cggttaatgt tcgactttgt ctgcaatcat atgtctgccg aaagtacttg
     2281 gtttcaggaa tatttaaact tgaatgatac gtacaaagat ttttttattg atttgccact
     2341 ggatacagat acaagcagcg tgactagacc aaggaccagc ccgttactta caacgttcat
     2401 tgacaaaaac gggaaagaaa aacacgtatg gacgactttt agcgaagatc aagtagactt
     2461 gaattttaaa aatccagaag ttttattgaa aatgatcgat attcttttgt tttacttaga
     2521 aaaaggcgct tcatttattc gcttggacgc tgtgggattt ttatggaaag aactaggaac
     2581 ttcttgtata cacctgccag aaacgcataa aatcatccaa ttattccgtg caatcgctga
     2641 tgaagcagct ccaggaacgg tattgatcac tgagacaaat gtgccacaca aagataatat
     2701 cagttatttt gggaatggcg agaatgaagc acagatggtc tatcagtttt cgctaccacc
     2761 tttgaccctt caagcgattc gttcgggtaa tacaaataag ttgaaagaat ggttaaaaac
     2821 catcgatttc gcatcgaaaa aaacaacttt ttttaatttt ttagcctccc atgacggtat
     2881 cggattgaat ccggtacgag gtattctgcc ggaaaaaacg atccttgaat tagtggaaca
     2941 gctgaagaaa gagggtgccc gagtaaacta caagaaaaac ccagatggaa cgaccagtcc
     3001 atatgagata aacgctacgt atctagatgc gttaagttct ccaacagata cgacggaatg
     3061 gagaagcaaa cgctttttag ctgctcattc atttttggta acgttaccag gcgtgccggc
     3121 aatttatatt caaagtgtgc tgggaagtcg aaatgattac aaaggcgtag aaaaaacagg
     3181 agagaatcga aaaatcaatc ggcaaaaatt tttgttagca gatattgaga aagagttgca
     3241 gacagatcca ttaagaacag cagtatatgg tgaattgacc cagatgctat ctattcgaaa
     3301 aaaagaaaaa gccttccatc cgaatagttt gatttactta ctggactcag cagattcttt
     3361 tatcagtttt attcgcgaag cgaaggaaga gaaagtctta gttatccata atttttccaa
     3421 tcaatcagca ggttatcttt taccaaaagg agattaccta gacctgtttt ctggacaaat
     3481 tatccattgc agtcaagaac gaaagttgat attccagccg tatgaatata tgtggctgaa
     3541 aataataaaa taagaggtgg aaaaaaatga aaaaaattat tgttactacc tgtctgctag
     3601 ctactggatt tcttcttact gcttgtggtg aaagtcaaag ctctaacgaa tcacaacaga
     3661 caacgattca attcatgcac tcttctgtcg aacaggaacg attaagtgtg atcaatgaac
     3721 tggttgctga ttttgaaaaa gaaaatccag acatcaaaat caagcaagtg ccggtagaag
     3781 aatcagcgtt caatacaaaa atcgttacgt tagctcgttc cggtaaatta ccagaagtaa
     3841 tggaagtcag tcaggatttt gctaaagtga tggataaaga tgaactgatt gatcagacgg
     3901 cagtccaaaa cgttatcgag gaagctggag aagataatta ttatgacgga gccaaaaacc
     3961 ttgttcgttc agaagatgga aaaagctata ttgctgcacc aattagcggc tgggttcaag
     4021 gtatttggta tgacaaagaa aaattatcag aagctggttt ttcagaacca gaaaattggg
     4081 atgatatttt aaaaattgct caacatttca cagataaaga gaataaacaa tatggaattg
     4141 ctatgcctac tgctgaagga acaatgagtg agcaagcgtt ctctcaattt gctttgtcaa
     4201 atggagcaaa tgttttggat gatgaaggcg aggtaacgat cgatacggaa aagatgagag
     4261 aagcattgtc gttttatcaa aacttatccc aatacacgat gccaggctct aatgatgtga
     4321 cagaaatcaa agatgctttc atgaatggaa cagcaccaat ggcaatctat tcaacttaca
     4381 ttttgccttc tgtttatgaa gcggggaact cagaaaatat cggttttgct atcccaacac
     4441 aaaaagatca agctgtctat ggcacagtat ctggattgac gatttcttct ggtctagacg
     4501 agaaacaaaa agaggctgct gaagcattca cagctttctt atctcaaccg aaaaacatgg
     4561 aaaagtgggt cttgatgtct cctggtggtg ctcaaccagt gaataaacaa gtagtcgaac
     4621 tggatggtta caaagaaaat gaagtcatca agtcttttgg cgagctgcca tctgagattg
     4681 ctagtgcttt tgatgaagta caagtctttg gactagtcgg ggaaaagaat ttcacgaaaa
     4741 tgggagatat cacaagctca ggtgtgatcg gaaaagcagt gaaccaagta actgtcggaa
     4801 acgaagatgt cgatcaagct ttagctgacg cacaaaaaaa tacgaaataa caagaagttc
     4861 tgccaatggt gttcgaaaaa agtagatagc tattggcaga ttatcagggg gaggtttcaa
     4921 tgaatcgtcc aaggaaacat tctgacttaa aattagccat gggactttta gcaccaagca
     4981 tgcttttatt agctgtttta gtgctgattc caatgctttc caatattcag atcagttttt
     5041 tcgatatgcc gctgaatcct aactcagacg ccgtttttat cggattaagc aattatcttg
     5101 aaattttaag tgatagtgaa ttttatcgtt ctttaggttt aacagttgct tacacattct
     5161 tagttgtagc aggcagtaca gctttaggat tagccgtagc tattttgctt aaccgcaagt
     5221 tcagatttag gaaaacggtg cgttcactga ttttattaag ttatgttacg ccatctattt
     5281 ctttgatttt tgcttggaaa tatatgttca ataatagtta tggaatcgtc aatgatctgt
     5341 tagtagatag gttgcagctt ttcaacgaag caccgctgtg gttcgaccag ccagttgcta
     5401 gcttctttat ggtcgttttg tttgctatct ggagatacta tccgtatgcg tttatttcat
     5461 ttttggctat tttgcaaaca gtcgacaaaa cgctttatga agcagctgaa atggacggtg
     5521 caaatgcttg gcagaagttc aagatcgtga cactgccggc aatcaaacca gtactagcaa
     5581 ctgtgattgt tttacgatct atctggatgt tctatatgtt tacagatgtc tatctactga
     5641 cgaataaagt caatatctta ggcgtttatt tgtatcagac agcctttgcg ttcaatgatc
     5701 ttggaaaagc agcagctata tcgattctat tgttcatcat tattgcagcg gtcgtcttgc
     5761 tctcacgaaa gaaggtggac ttgaaatgaa aaataaaaag agcaggaaaa tcttatttta
     5821 cgcgacgatt ttgttgctat tagctgtcaa tttgtttcca tttttgatca tgctgatgac
     5881 atcattcaag gattcacaag aagcaattgc aacgacacaa actatttttc cgaaagaatg
     5941 gacttttgaa cattatcagg atattttcaa tgctgagatt tttccattca tcatgtattt
     6001 caaaaatagt ttagtgatct ccttgatagc ctccgtaact gcagtagtga ttgggatcag
     6061 cggggcatac gctttgtcaa aactacgctt caaagctcgc tcgaaaatca atgctagctt
     6121 ttacaccgtt tatatgtttt ccggtatttt gatcgttgtt ccattattta aaatcatttc
     6181 cggtctaggt ttgtatgata ccaatacagc actgattatc acgatggtcg ttcaaacact
     6241 tcctactgcg atattcatgc taaaaagtta ttttgacacg attccgactg atattgaaga
     6301 agcagcaatg atcgatggtc ttagccggat ccagatcatt attcggatca ttataccatt
     6361 ggctatttct ggaatcattt ctgtcttcgt ttattgcttt atggtttcgt ggaatgatta
     6421 tttatttgct tcgattttcc tgtcgacctc cgaaaaattt actttaccaa tcggtttgaa
     6481 cacattattc agtacaccag actacatttg gggacgaatg atggccgctt cattggtgac
     6541 cgctttacca gtcatcatta tgtatgcgtt atctgaacga tttattaaag gtgatttagt
     6601 cgaaggcggc gtaaaaggat aaaaagaaaa gaaagaggaa agaaaagatg aaaaaattag
     6661 ttgcattaga accaagaata gccggattca aagattacga agaccgtgag atcgaaaaga
     6721 acgaagtacg tatccgagta attttcgcta ctccaaaacc aggaacagaa attgtcgact
     6781 ttagaggatt aagtccattt atcgatgaag aattttcaga agaatggcgc ctattcttgc
     6841 caagagaaga aggaagccaa cgcggtgtgg tatttggcga atttcaatta ggaaatatga
     6901 ttgtcggtac gatcgaagaa acgggcagtt tggtgacaga gtatcaaaaa ggagagttag
     6961 tctgcactta tggacccatt cgtgaaacgc tgattacaaa cggtgttgat aattataaac
     7021 tacgtaaact tgatcggttg gatcaatgga aaaatgcagt atgttacgat cctgctcaat
     7081 tcgctttagg tggtatccgg gaagcacatg ttcgagcagg tgatgctgta gcaatcttcg
     7141 gtttaggcgc tatcgggcaa attgcgattc aattagctaa aaaagcaggt gctactacca
     7201 tcgttgggat cgatcccata gcgcagcgta gagaattagc agaaaaacac ggtgccgatt
     7261 acacgctaga tcctatgaaa gcagatgtcg gttttcgtct aaaggaattg acagataaac
     7321 gtggagtaga ttcagttatt gaaacgagtg gcagtgctgc cgcattacaa gcttctttaa
     7381 tagggatcgg ctatggcggg atcatttctt atgttgcctt tgctaaacct tttccagctg
     7441 ggtttaatct tggtcgcgaa gcacatttca ataatgcaaa aatcgtcttt gctcgagcat
     7501 ctagtgaacc aaacccagat tacccaagat ggaaccgtaa acgaatcgag gacacatgtt
     7561 gggaattatt gatgaacggt tacttggatt gtgaagaaat cattgatcca gttgtgccat
     7621 ttgcagaaag cgcagaagct tacatgcgtt atgtagacca gcacccagaa gaaagtatca
     7681 aaatgggcgt tcaagtccga ggaggagaac aatgaaattc ggtacgcaag atcaagcatt
     7741 tttcccaaga gacattttag aaaaatttag atttatcaaa gactgtggat ttgaagaata
     7801 cgaaatcgac ggtaagttgt taatagaaaa tatcgacaca gtcaaacagg caatcaaaga
     7861 aacagggatc ccagtagtga cagcttgtaa cggctacgat gggtggatcg gtgactttat
     7921 cgaagaacgg cgtcttaatg gtgtacgtca aattgctgag attttgcgtg cactaagcga
     7981 ggttggaggc aaaggaatcg ttatcccagc agcttgggga atgttcactt atcgattacc
     8041 gccgatggtt tcaccccgaa gcaaagaagg agactataaa gcagtcagcg aatcacttcg
     8101 ttatttagat aaagtagccg aagaaacagg tacggttatt tatttggagc ctttgaaccg
     8161 ataccaagac catatgatca atttgttagc ggatgcgcgt cattacattg actctaatca
     8221 attgaaacat gtgcagatca tcgctgattt ttatcatatg aatattgaag aagatgatat
     8281 ttcaaaagca ttgactacgc aaaaagagta tgtgggtcat atccatttag cagataatca
     8341 tcgctaccaa ccaggatctg gcgcaattga ttttactcgc cattttcaaa cgctacttca
     8401 aaatgagtat gaaggttata tggtatatga atgtcgtttg cgaggagaaa atgaagtgaa
     8461 aatttatcag gaatcattgc aatacttgaa aaactgtctg gaaacagcgc gtcatttaaa
     8521 tgatttagag aaagaataga gaaagaataa caataagtaa ataagcaaag gagggagaaa
     8581 taaatgaaga aagtagcaat cataggcgct ggacaagtag cagaaaaagt acatgctgct
     8641 tattatcaaa aaagatcaga tgtcttagaa ttggccgctg tggttgatcc agtcagccag
     8701 cgagcagaag acttttgccg acgtacagga ttttctcatg cgtatacatc agttacacaa
     8761 atgctgaatg agatttctcc cgatatcgtg agtgtctgta cacccaatcg ctttcacttc
     8821 gaacatgtat tggctgccct cgaagctaat gcttcagtga tgtgcgagaa accaccagct
     8881 atgacagctg atcaagcaag aaagatggca gagacagccg ataaaaacgg gcagattttg
     8941 gcttatggat tccagcatcg tttttctgac gaagcaacaa tgatcaagaa tcatcttgat
     9001 gttctcgggg atatttatta cgtagaagcg aatgcgcttc gtcggtctgg tgttccaggc
     9061 tggggaacgt ttatcgacaa agaacttcaa ggaggcgggc ctttgatcga ttatggcatt
     9121 cacatgcttg atacagcgat gtatattact ggatttccta aagtaaaaag cgttcgggcg
     9181 tacagcttcc aaaaaattgg tacgaaaaaa aattcaggga aattcgggaa atgggatcca
     9241 agcaaattca cagtagaaga tgcaattttt ggcacactgg aattagaaaa cggcaagatc
     9301 atccaactga atacttcgtt tgctttgaat atcagagaag acaaactgaa tgtggctttt
     9361 tgtggcgatc aggcaggtgc taatctgtat ccaggcgaac tattcgaaga tgtagatgga
     9421 aagctggttt ccttacaaaa gaccacgcca gttaatgacc cacatgaact aagtatggct
     9481 cattttgtga atcgggcgct cggtgaccag actcagatga ttgcagatgg ctggcaagga
     9541 tatgtcatcc agaatctggt ggaaaattta tatcgttcgg cagaaacagg agaagttatt
     9601 accttatgaa agtagaagaa aaacagtttg atttagagta tatcaataag tatgcgagtc
     9661 agatgaccat tggaaatgga ttttttggta tcagaggaag ccaggaggaa gactatcgga
     9721 atcaagtaag gggaatgttt gcagctggtg tatataaccg acctttagga gcagaatcag
     9781 ccgagttagt gaatctccca gatgtcatca gatgggaaat aaaaatcgac ggtgaacatt
     9841 tttctcttga gacgatgaaa gttcttgcat acgagcgggc actagattta gaaatgggag
     9901 agcttaaacg ctatgcgctc attgaaactg ctcaaggaaa aaaagtggaa gtcattactt
     9961 gtcgtgttgc tgatcaaaat aatctccatc aggcagccct gaaagtggtc atcaagccgt
    10021 tgaccgaaga ctgccaagta gatgtgaaaa cgggtatcga tggacagcaa accaattttg
    10081 gtacacagca gttggttgaa aaagaactgc gagtatttgg tgacgatttg atcagtgcat
    10141 cctaccagac aacagaaagt aaaattgata ttgggattgc agctaaattc aataaagcag
    10201 gtgtattcca agcaaaaaat cgccggatca ccgcttcctt ttcagaaaag atcagaaaaa
    10261 atacgtcctt tacgctggag aagatcatcg tgctctctac ctctcttcag caaacagacc
    10321 cgtttaagaa aagtattgcc caagcccaga aaagcgttag ttatacagaa attttagagc
    10381 agtcgatgtc ttattggaca gatttttggc atcagcatcg gataacgatc caaggcgatt
    10441 cttctttcga tcagttagcg atggacttcg ctgcttacca tttacagatt atgactcccc
    10501 gcaatgatcc aagtgttagt gtaggggcga agggactaac tggcgaaggt tataaagggc
    10561 atgttttctg ggatacggaa atttttatcc tgcctttctt tttatatcag tttcctgaag
    10621 tagcaaagaa tttgctgctg taccgttatg atcgattaga aggcgcacgg caaaaagcaa
    10681 gagattatca ctatcaaggt gcacttttcc catgggaaag cgcacacact ggtcaggaag
    10741 agacacccaa atttgctgca ttgaatatca aaaccggaac aagacaagaa gttgcttcag
    10801 ctgccgcaga acatcatatc agtgcagacc ttgcttatgc cgttcaagag tatgtccaag
    10861 caacagagga tcaatcgttt ttagaaacaa agggaaaaac gctcattcat gaaacagcag
    10921 ctttttggct gagcagagct gtaaaagtcg atgatcattt agagatacac agagtcattg
    10981 gtccagatga atacacagaa tatatcgaca ataatgcata tacgaactat ctggcgcatt
    11041 ataatgtggc cttagctgtt aaatatcaag ctggcgatcc gctttttttg aaagaatgtc
    11101 aaaatttttt agataagtta tatttgccag tgcctaacaa agaacagctg attccgcaag
    11161 atgacacgta tctcagtaag ccagttattg atttgaccac ttacaaagaa cgtcctggca
    11221 gccagtctat tttacttgat tatgcccgta gcgaagtaaa tgaactccaa gtcagcaagc
    11281 aagcagatct tgtcatgctg ttatacttat tacctgagta tttttctcaa ccaatcgttt
    11341 ctaagaattt gcattactat gaaaacaaaa caatccacga ctcatcgttg agtaaagcga
    11401 ttcacgccat agaggcttgc cgtatccaag aaatggaatg ggcgtatcat ttattccaag
    11461 aagcgtgcct gattgattta ggaccagatc cgcattcatc agatgacgga attcatgctg
    11521 cttcattagg agcactatgg ctagcagtta tatttggttt ttcaggtatt acagtaacga
    11581 atggaactct ttctatttct ccaagacttc cttcagcatg ggaaaaaatt tcttttcctt
    11641 ttatttggaa aaatcacagg ttgctatttg ttttgacaaa aaaacaaata gtaataaaaa
    11701 aagagacgaa agaaacatta cctatttttg tcaatggaga ccaataccag ctggaaaaag
    11761 aactgatctt ggaacggagg ggaaaataat gcgtattctg attgcgccag attctttcaa
    11821 agaaagtctg acagccttag aggcagcaca agccattcaa tcaggttttc aagaagtttt
    11881 tgttcaggct gaatatgatc tgctacctat aggcgatgga ggagaaggaa cggtttcatc
    11941 attagcagac agtttaaggt tgaagcggca aacagcagtc gttactaatg catttggcaa
    12001 aaacaaagaa atccagtacg cttcagacgg tcaaactgcg atttttgaaa tggcagaaat
    12061 cgtcggattg aaagatgtcc cactaaacca acaacagcca cttaggttat ccacgcgagg
    12121 agtaggagaa ctgatacgac aattgttgca acaaggtgtt cgacacatca tcatcggcgt
    12181 tggcggaagt agtaccaacg atggcggcat cggcatggca gaaggtctag gttatgcttt
    12241 ttacgatcaa gataatcaaa aagtagaagc actgggagaa aatctaggta aaattgctac
    12301 agttgataaa acgaacgtgc ctattgagct gattaaagcc aaaatcactg tcattactga
    12361 tgttactaat cttttatgcg gaaaagaagg cgcttctttt gttttcggac cacaaaaagg
    12421 cttgaaagaa aatgaattag cagtggctga ccaagccatg catgattttt atatgagatt
    12481 tgcccctcaa gtaatcgagg catcgggcag tagtgctggt ggtggaatgg gtgcaggact
    12541 gttagcattt gcttcaggga aaatagtctc tggcatcgac tacgtattgg atttactgga
    12601 gttcgatacg ggtacagcaa gcagacctcg taatcgtagg cgaaggacgt ctagatggac
    12661 aaagtctaaa aggaaaagcg ccagttggga ttgctagaag agtaccagag ggcgttcctg
    12721 tgatagctat ttgcggaagt gttggtaaag gaagcgaaaa agcagcagat gttggcattt
    12781 cggccgtttt tccgatcctt cctgcagctg tgcctttaca agaagcttta cagcaagcca
    12841 ctgataatct tcatcgcaca gctaaaaaca tcgcatcttt acttcaagtc gttcattttc
    12901 aaggaggtaa tcgatgaaaa tcaaagcagc actattcgat ttagatggcg ttcttgtaga
    12961 cacagctcgt tatcattacg aagcgtggct ggttttagct aaccagctgt ccattccatt
    13021 cactgagaaa gaaaacgagc aactgaaagg aatatccaga accgagtcac tagagcgatt
    13081 gctgtcattt ggaaaaatgg aacagaaatt ctcagaaaaa gagaaatcag cctttgctga
    13141 gcaaaaaaac aatctatatc ttcaagcgat tcaaaaaatg gatgaaacat ccgttttgcc
    13201 tggtgcaata gctgttcttg agtacttgaa aaaaacaaac atcaagatcg gattgggatc
    13261 agcaagcaaa aatgcacgcc tcattttaga aaaaacgaat ttgacaagtt attttgatgt
    13321 ccttattgac ggcacgcaag tatcaaaagc aaaacctgat ccagaagttt ttctcaaagg
    13381 agcacagcaa ttgaatgtac cgccaaatgc atgcttggtc attgaagatt cagaagctgg
    13441 ttgtcaagca gctttggctg gtaacatgca tgtattggga ataggtgaaa acataaatct
    13501 tccttctgct gaatatgtga ttcctgattt aaccgttttt gatcaagtac gttcgttttg
    13561 gcatttatca gaagccgtat cacgtatata atagtttccg ttttttctgt cacaagcaaa
    13621 acgttatcct ccttttggaa atcaaggtat gatgagaaaa aagggggatt ttatatgaca
    13681 aaaaaagcag ttttcgcagg cggatgcttt tggtgtatgg tcaaaccatt tgaagagcag
    13741 ccagggatca tttctgtgac atccggttat actggcgggc atgtaccaaa tccaacttat
    13801 gaacaagtaa caacaggcac aacagggcat acagaagcag tagagatcga atatgatcca
    13861 gaagttattt cttatgatca gttggtggaa atctattggc agcaaaccga tccgactgac
    13921 gcattaggac agttcgctga tcgcggtgat tcttatcgtc cagtgatcta ctataatgat
    13981 gaagaagaac gaaatattgc agagcgttca aaacaagcat tgcaagacag cgggcgattc
    14041 gataagccaa tcgtgacaaa aatcgaacca agaagcgaat tttatcctgc agaagattac
    14101 catcaggatt attacaagaa aaacaagctc cattacaatc tttatcgaga aggttctgga
    14161 cgtgcaggtt tccttcgtcg acattggggc gtcaagaaat aagaagccga aaaagctact
    14221 tatggaacac cgtatatcag aggttgtaaa accagcattt tgagctaata ctgagggtca
    14281 gctgattgaa caccatttag tggagaggtt gtgaacctgt cgcctagctc caagtcacaa
    14341 ggatttggta agagaacact atatatcaga ggttgtgaca agtcgtttgt cacaaccttt
    14401 tttcaatacc taagatgaag gacaatcagc aacgcactgc tcaaaaaagg aatgaaaggc
    14461 agcttttcca actttttttt atgacagata aagtaaacag aaaaaacgga tattccaaac
    14521 agactagcaa gaaacaagat ctgaagcaac tcttctagag acaagccacc actccaagaa
    14581 agcatcaaca gagtatcgcc agctccgaga taggattgac catagcgaag gcacacaccg
    14641 cctacgataa acgttaacag cacactttgc cattggaaga cgccggcctg caaccaaaaa
    14701 atccataaaa ttgcccaaga acagaatagc gtgtgagaat ggaccatgaa gtaaaataaa
    14761 tccatcaacg aaaatagcaa agccgaaagc atccatatga acgtcgaaaa atttcttgcg
    14821 gaagaaaaag taaagaacca ggcaaaaaga ccaccacata aggcttctgc caacaaatag
    14881 tagaagggca accgacacct gcaatagcgg cagcgaaatc gttggacgat gatcgagaga
    14941 atcggaatca attcatacca agaaagcgaa cgacagcatt tgacacaatg agatctagga
    15001 taaataatag agtgacctaa cggaatccgc tgggcggtta aacataaaaa tgatccgata
    15061 cagcaaccaa tgataaaata aagtaacacg ccatctcccc cttttcaaaa ccaagatacg
    15121 caagcttaaa ctaaattttt gtacatacat aaaatttgag gtgaaaaaat gaacgtttca
    15181 atcggattag atatcggaac cacacaaacc aaagcagttg ctttcacaga tgaagcccaa
    15241 gaagcagcat ccgcttattt tcgctaccct ttgatccaag aaacacaagg gatggcagaa
    15301 caagatcagg aattgatctt tcaaggtgtc tgcaccgtta taaaagaagt aattaaacag
    15361 ttgccttcag acacaatcat tcaatttgtc tccttctcct ctgcgatgca tggcctgata
    15421 ttgttggatg aagagaaaac accgttatct cgaatgatca cttgggcaga taaccgagca
    15481 gcggacgaag cagaaaaact gaaatatacc gtgcttggca aagaatttta tcggcagact
    15541 gggacgccga ttcatccaat gacaccattg aacaaaatca agtggctgca aaacagccaa
    15601 cctgaattat tcaaaaaaac tgcttatttt gtcggtatca aagactatat cttttataga
    15661 ttgtttggcg aactgatcag cgactacgga acagcttcag gaactggata ttttgatatc
    15721 catgaattcc aatgggcaga agaagtgctt gactacttga acattcgaat agagcaattg
    15781 ccgcaagtct ttccttccac ctatcaagta acaggtctac catcaaaaac agcagcagaa
    15841 ctaggtttgc caaatgatat atcatttgtt ttgggagggg cagacgggcc gctttctaat
    15901 ctagggttag gtgcttttgg tgatacgatc gccgctttga ccgtaggaac gagcggagca
    15961 cttcgtttta ttactactca gcctcagttc catccagaaa tggagacgtt ttgttatgtt
    16021 ctggatcaga aacattgggt agtgggagga gcgactagca atggtgctgg catctttgat
    16081 tgggcagggc atacattttt acaggaagtc gttcaatctg ctaaagaaaa aggagaaaat
    16141 ccatatgatg cactgctttc ggctatcgct tttgtgccag cgggtgccaa tggtctgatt
    16201 tttcatccct atttacttgg tgagcgagca ccgctttggg acgcagatgc gtttggcagt
    16261 tttatcggtt tacagcggca acatacagaa aaagaaatga tgaaggcgat tctagaaggg
    16321 atctgtctca acctttatcg gattttaaaa ggagtttgcc aggaaaatca gctcacagag
    16381 atccgtgcta caggcgggtt tgctcaatct ccgatttttc gtcagatgat ggctgatgta
    16441 ctgggttacc cgcttgtttt ccctactgtc acagaagcat ccgcattagg agctgtgatt
    16501 ttaggctgga gaagcatcgg aaagatccaa agtatcagtg aagcacagga attgatagaa
    16561 ttgaaagagc aagttggagt acaagaaact gatcataaaa aatatcagaa aatctatcca
    16621 ttatttgttt cgacacaaaa gcatttagca cagacttatc agcaatttgc tcaactgcga
    16681 gaagaattag ctgattctga aaaatgattg gctttacgga taaaaaagcg aactgaaaat
    16741 cgttctcagg taaatgattt tgttgtactt ttcattgaaa gtctactatt attttaagtg
    16801 taagaaaatg ttaggaggca agaaagatga aattccaaca aaccaaagaa gtattaaatc
    16861 aattagttgc agatctaagc caaatgtctg ttgttgttca ccaaacacat tggtacatga
    16921 gaggaccagg tttccttaca ttgcacccaa tgatggatga gttcatggat gatttgaacg
    16981 ctcagttaga tgaagtatcc gaacgcttga tcacattaga cggttcacct tattctacat
    17041 tgcgtgaatt tgcagataac acaaaaatcc ctgatgaaat tggccgctgg gatcgtacta
    17101 ttccagaacg tttggaaaca cttgttgcag gctatcgtta tctagcagat ctttaccaaa
    17161 aaggaatcga agtttctgga gaagaaggcg acgatccaac acaagatatc tttatcgaat
    17221 tcaaaacagc cacagaaaaa cgtatctgga tgatccaagc tcatttgggc aaagcgccag
    17281 agatcgacgc gtaaaaactg ttgttgaact tgaccgaaaa agaggttgtg aaatcagcgt
    17341 tttgacctga gtattagaga aacaaacgtc aaaatagttc ctcatatttt gccgtttttt
    17401 gagctaatac cgagggtcag ctgattgaac accgtttacc aagaggttgt aagcctgccg
    17461 ttcagctccg agtcacaaga aacaatttta aaaaacagct tcttatgttt tatggatatt
    17521 gtgacttgtg accgaggagt tggcaggaga acaccgttta ttcaaaataa ggggttgtga
    17581 caagactttt gtcacagcct ctttttgttt ttcattttga caggaaacga cccttttata
    17641 taaaagaaag aaaagaagta aagtttcata taaatttttc aatcaagcag aaaaacgttg
    17701 acattgacta gattagcggg taatatgtta aatggtattg tttgccccac aaagagcccc
    17761 ggaaactcga agtgtatgtc aaacatcaga acttaaattg gaggaaaata acgtgcgtac
    17821 aacatatatg gccaaaccag gcgaagtaga acgtaaatgg tatgtagtag acgcaactga
    17881 tgttccattg ggacgtcttt caacagttgt tgcatctgta ctacgtggta aaaataaacc
    17941 aactttcaca cctcatttgg atactggcga tttcgtcatc gtcatcaatg cagacaaagt
    18001 gaaattaaca ggtaaaaaag cgactgacaa aatttattac cgtcacagca actacccagg
    18061 aggattgaaa tcaatcactg ctggcgaatt gcgtgctaaa aattctcgtc gtttgatcga
    18121 aacttctgta aaaggtatgc ttcctaaaaa cactttaggc cgcaaacaat ttacaaaatt
    18181 gaacgtatac ggtggagcag aacatccaca tgcagcacaa caaccagaag ttttagacat
    18241 cacaaactta atctaatagg agggaatttc attggcacaa gttcaatata tcggcacagg
    18301 ccgtcgtaaa aacgcagttg ctcgcgtacg tctagtacca ggaactggca aaattactgt
    18361 aaataaaaaa gatgttgaag aatacattcc acatgctgac ttgcgtgaag taatcaatca
    18421 acctttcgct gttactgaaa caaaaggcgc atatgacgta tttgttaacg taaacggcgg
    18481 aggatacgct ggacaatcag gagctatccg tcatggtatc gctcgtgcat tgctagaagt
    18541 tgatcctgac ttccgtttag ctcttaaacg tgctggctta cttactcgtg acgcacgtat
    18601 ggtcgaacgt aaaaaaccag gtcttaagaa agcacgtaaa gcttcacaat tctcaaaacg
    18661 ttaatatcct ttatatatca acgtttctgg cacttttcaa ttatttggaa agtgcttctt
    18721 ttgtttttaa gaacacattt aagaacacat atcaatcaca cttattcaat cctctctgaa
    18781 aagcttcaaa aacagcctta tcaactatat ctggtgtgtt cacatatatc tttgttgtag
    18841 aaatatctga gtgtgttaaa gcatttgaaa tttgattcat atctgctcca ccttgtctag
    18901 ctatagttgc ataagtatga cggagtttgt gtggagataa atgaactaag tcaggatgcc
    18961 ttcttttaat tgaattgatt cgatagttta ggtaatcggc atgaacagga caatttactt
    19021 ctccagatgg tttagtataa gtaaagagat actgatctga cgtttgctta atttttaatt
    19081 tcaacaattg atcagcttgt accgatttcc attcacttag caatgtcatt acaacttctg
    19141 gtacttgaat gacggtatct tttcgacctt ttgtaaactt ttttctttga tatttatcta
    19201 aagtatgttt aagacggacc gtttgttttt caaaatcaat atatttccat tgcaaagcgt
    19261 aagtctcact ttttctatca ccaagataca gggtaatcat aaaaagtgtg taatcatgaa
    19321 aagtaagtaa atgattatta aaatcatcat ttactgcttc tatccaatta gtaagttctt
    19381 ttgatgatag tgcttgctta ccttcttgta ttcttttttc ttctaaagca ttttttttag
    19441 gtgcagtgat actttgaata attttagtag ttcgattaaa ttcgatataa tttagtattt
    19501 ctgcaatatc gaacatggaa cgaacatatc cttttatgat ttttatattt gcatattctt
    19561 tcgatttttt ggttaattca tctgatatta tctcagtatt gatatttaga taattcattg
    19621 cgtattcacc aaacataggt aaaatatgca accgaaatat atcttttgta ttactaatgg
    19681 ttatatcact aggaggcctg tttgatctaa ttgtttgacc taattcatat ctaggaagcc
    19741 atttagattc gtaaaatttt ttaaacgtta tttttccttt taattctaat gagtttgcat
    19801 tctcctctct caaaactttt tctattttct tcttcatatc attttcggct ttaatcgcca
    19861 attgcctatt tggaaatgtt ttttgatact tttctgatgc aattcctgtt atttctttaa
    19921 acctttttgg atatctcaga ttagttttat atttcccatt tgaaagcttt gtaggcaaaa
    19981 tcctcactcc ttaaaaaaag taaggcagta tatttcacta attttagtat atgcggcgag
    20041 aagaaaattt caagttattt taaatgtatt cataaagtct ttaatatctt ctacgtcaaa
    20101 gcgaactaca ccaccaactc ttattactgg gaaattatat atacgaatcc acttatctag
    20161 agtattatta tcgattccta gaaatgcaca ggtttgtttt ttattcaggt agactctgga
    20221 ttctataaaa tcatctccta aaatagtagt tatgaaattt ttgaattgct ttgggtaagc
    20281 acataggaat ttcacctctc cccagtttac ggcgagcctt acggaagtat cattatttgc
    20341 tgctattgtc atggcatttt gttttcccag aacaaaaaga aatctctaag tatttgtcgc
    20401 taatcaggtc atggcgtact tgagtttcta gctcgaatag cagggatcgt tattcaaaaa
    20461 ttgtatttaa ttgtcaatga tcaaaaatag tttttttcct attcactatt aataacgata
    20521 aacatgtata aaaagacaca ctacgaaaaa agttaaatgg agtttactga ttttcatatt
    20581 tgtatatgga acagacatag tcttgaataa tttttataaa tgttttgatg cttatcaact
    20641 tattcaaccc gctatccact tcaagtataa tttttccata cgccaataaa gtagttttag
    20701 gagcatgttc tcatttgaga acgctccttt tttatatatt ttgaaagcta cttactatac
    20761 gcgtagtttc aaatagtttt gtcattctaa tcttcagcaa cagaaa
//
LOCUS       DAXWLF010000033        20789 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXWLF010000033 DAXWLF010000000
VERSION     DAXWLF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.033"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(286..459)
                     /locus_tag="ACGE4U_000946"
     CDS             complement(286..459)
                     /locus_tag="ACGE4U_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393128.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(462..860)
                     /locus_tag="ACGE4U_000947"
     CDS             complement(462..860)
                     /locus_tag="ACGE4U_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393129.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1076..2131
                     /locus_tag="ACGE4U_000948"
     CDS             1076..2131
                     /locus_tag="ACGE4U_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393130.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2151..2504
                     /locus_tag="ACGE4U_000949"
     CDS             2151..2504
                     /locus_tag="ACGE4U_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393131.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2686..2973)
                     /locus_tag="ACGE4U_000950"
     CDS             complement(2686..2973)
                     /locus_tag="ACGE4U_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393132.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(2986..3804)
                     /locus_tag="ACGE4U_000951"
     CDS             complement(2986..3804)
                     /locus_tag="ACGE4U_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8393133.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4030..4251
                     /gene="relB"
                     /locus_tag="ACGE4U_000952"
     CDS             4030..4251
                     /gene="relB"
                     /locus_tag="ACGE4U_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFF8393134.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4253..4519
                     /locus_tag="ACGE4U_000953"
     CDS             4253..4519
                     /locus_tag="ACGE4U_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8393135.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4686..5171
                     /locus_tag="ACGE4U_000954"
     CDS             4686..5171
                     /locus_tag="ACGE4U_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393136.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVGTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKLQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     T"
     gene            5380..5562
                     /locus_tag="ACGE4U_000955"
     CDS             5380..5562
                     /locus_tag="ACGE4U_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8393137.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            5665..5949
                     /locus_tag="ACGE4U_000956"
     CDS             5665..5949
                     /locus_tag="ACGE4U_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393138.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            5965..6201
                     /locus_tag="ACGE4U_000957"
     CDS             5965..6201
                     /locus_tag="ACGE4U_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393139.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6223..6477
                     /locus_tag="ACGE4U_000958"
     CDS             6223..6477
                     /locus_tag="ACGE4U_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393140.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            6559..6744
                     /locus_tag="ACGE4U_000959"
     CDS             6559..6744
                     /locus_tag="ACGE4U_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFF8393141.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            6811..7206
                     /locus_tag="ACGE4U_000960"
     CDS             6811..7206
                     /locus_tag="ACGE4U_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFF8393142.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7223..7573
                     /locus_tag="ACGE4U_000961"
     CDS             7223..7573
                     /locus_tag="ACGE4U_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393143.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            7595..7969
                     /locus_tag="ACGE4U_000962"
     CDS             7595..7969
                     /locus_tag="ACGE4U_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393144.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(8276..8674)
                     /locus_tag="ACGE4U_000963"
     CDS             complement(8276..8674)
                     /locus_tag="ACGE4U_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFF8393145.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(8866..9633)
                     /locus_tag="ACGE4U_000964"
     CDS             complement(8866..9633)
                     /locus_tag="ACGE4U_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8393146.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(9668..10828)
                     /locus_tag="ACGE4U_000965"
     CDS             complement(9668..10828)
                     /locus_tag="ACGE4U_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8393147.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLENHFTQYTFDQVV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            11872..12675
                     /locus_tag="ACGE4U_000966"
     CDS             11872..12675
                     /locus_tag="ACGE4U_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8393148.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            13260..13715
                     /locus_tag="ACGE4U_000967"
     CDS             13260..13715
                     /locus_tag="ACGE4U_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393149.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            13834..14892
                     /locus_tag="ACGE4U_000968"
     CDS             13834..14892
                     /locus_tag="ACGE4U_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFF8393150.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            14934..16883
                     /locus_tag="ACGE4U_000969"
     CDS             14934..16883
                     /locus_tag="ACGE4U_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8393151.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            16886..20527
                     /locus_tag="ACGE4U_000970"
     CDS             16886..20527
                     /locus_tag="ACGE4U_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFF8393152.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         7279 a         3282 c         3688 g         6540 t
ORIGIN      
        1 taaatagctt aacctcgcta ttttgttttt tctgatcact atctatacag cattagtaaa
       61 tagaatttta aaaataaaaa aattaagaca aaaaaagtat caacataaac atatacatat
      121 acacgtttat gttgatacac ttaatttgtc ctttaaaaag cacttatttt aatattatgt
      181 tattttataa tcttattaca agctgattgt tagacgttgt cctttcatta catttattaa
      241 cggttatttt atcgtttaat ttattaatta tttttgatct aaaacttagc ttaacaaata
      301 aataattaac caaccgccag ataaaaatgc gatcattgct aatacataag caaagataaa
      361 aatatgttcg atcagcttaa accctgatgc gattaagcag accaagtaaa atagaaattc
      421 tcttacagat aatggtaatt ttctaactag catactcata attaacaccc caaagtaata
      481 ttgtattttg aacattttac atccaataca gcatcaatca accattgcca atcaaaaata
      541 caaggcttca ttgaagtatg tttttcagat gaaaagatat aatataattt cttcggaata
      601 atattttcag ttgttgggat caagctaaat ttaataccat ctaactcata gctaccagct
      661 ttctctccat taaaaatttc cttagcactt tcttctaaag aatcaaaata tttttcgaca
      721 gcaacctttg aagcttttac tattgaagca tttccctttt cgataaaatc tgcttttgtt
      781 ttaccgacaa taagtaattc ttgatcagta aaatcttcta aaataatagt agtgtaattc
      841 ttatttcttc tcaatcgcat caggatcact ccttaatata atttatgctt ttataatatc
      901 ataataagat aaatttgcaa gtgtttattt acaccttttt atttacactt tattgtaatt
      961 gattatactt ttttgattgc atttttttct tataaacagt tgcaactatt agtaaatagg
     1021 tgtaaactat tttcgtaccg aaataccata aacacaaaca gaaagaggtc ataccatgaa
     1081 agaaccatta ttgttttccg ttgacttagg gaataaatca gtaaaagaaa tgagtacaca
     1141 cgtttctcca ctctcgcttc cctctcgtat tttgaacaaa aatatcatta attcaggacg
     1201 tttttctaat ataggttcct cattaaaacg atcactaaac gtttctgaat atagcctagt
     1261 aaacactact gaaagctatt attttgggaa ggatatacac gagctaggaa aagatgacta
     1321 cgtgatagaa tcactaggtt ttgggatcag cagatacaag caggaaacct accaagatat
     1381 cctatctttc tctttagcta acctagcacg tttagaagat caatctgatg acgttttaac
     1441 cgttaatcta gttttaggtc tgccttcatc cgactatacc gaaacaatcg tcaaatacgt
     1501 gatgcaacaa gccacacgac aacattcagt tgaagttgac ggtaaagctt ataatataaa
     1561 cgtagatacc gtttgggtac tgccacagcc gattggaaca ctatataacc tcctccttgc
     1621 tgatgatggt agtgtacaag atgaatcatt gatcagtcag aagatcggtt ttgttgatat
     1681 tggtggtggc acgaaacttt ttgatgttgt ccaaaatttt gaaattgacg aaaacaaccg
     1741 tgaacaaaag gataccggta tttttacttt atataatcgt atcgtaaata tgcttgacct
     1801 tccgatcagt cctaatgctt tccaacttga aaagattatc cgagaaggca aagaaaaagg
     1861 cgtatatcta tttaaaccaa gcggaaatga aagttttgat atttcagaag tagttgaaag
     1921 agaaataaaa acatacacca atatggttgt tgcagaagtt ttagctacat tcaaaaactt
     1981 cacaacctta gataaagttg tctttactgg tggtggggct tctattatcg acaaacaact
     2041 tgtaaaagat aaaattccgc ataccttgtt tgttgaagac agtgaatttt ctaatgtaaa
     2101 aggcttttac aagtttggtt taactcaata aaacagcgaa ggagatcaac atgaataagg
     2161 gaaaatcaga gcgttatgta gttataatca atagagaaaa ggttagcgag ttacctttag
     2221 cagagtttct tgaatctgaa tatgaaacac taggcgttaa atactctcag acgatcaaaa
     2281 aagctttgaa tctatatcgg cgtgtaaaga acaatgaagt tacaattatt gaagcaggca
     2341 acgatagaaa cgatagcaaa gtagctgaag taaagaataa accaactgat cataacccta
     2401 ttcaacaagc gcatccttca acggttacat ccgatcattc agcaaagaaa gaaaagccaa
     2461 tgctaaaagg gttcaatgca ccaagtaaca aagacttact ttaaaaataa aaaggcgagg
     2521 ttacaatacc tagccttttt attatgtaca aatacaaaca taaacatata cgtatacatg
     2581 tatataaaca tgtatacgta tatatccata aacacgtata cgaaatttag ttaagctata
     2641 cctaaccgct tattccaata aaaaaagtga tcagctttaa actgatcact caccaaaaac
     2701 ttcatcatac aagttatcta tcatttcacc aagtaaagct gaatctgatc gaattcctaa
     2761 cttttcgctg attttagata gtttctttct ttgtgatggt tgaagcgtga attgataatt
     2821 tttcttcttt tcagaactat cactttttag tttagctacc aaagaacttg ttgtttcttt
     2881 aggtgtttgt ttaggttctt caactgcttc aaccacttca ggaacttctt ctacttcagc
     2941 ttcagcgaat tttttaaatc tattttcttt cacttcaatc actccttatt cttgtgccgt
     3001 atcaattatt ttgttgtata gcgtaaacgt gctttcaaag aaatccttat tcttacgata
     3061 gatattctta tccttagcga actcagcaat attcttacct tctaagctag ttgcattaag
     3121 caattccttc tcttgtacat atccaagata attagataac gttggtacca attctaggaa
     3181 ctcacgactt gaattagtat tatgtttgat cctgctaccg atcgtagcgt atttagtttc
     3241 catcagtgtt tcaccagtct taatatcaat caactgtttt gctagtgcat caatatcttt
     3301 accaagctta agcaaagctt caaaaccatc tttagatgga tcagaaatgc ctacgacaag
     3361 atcagataca gcccaagcat tttgagctag tagacctgca tcattatgag tatcaatcag
     3421 aattagatca tacttctctt taagctgttc atagttaaca acgatccatc taaacagtag
     3481 ggttctgttg ttaggcatat cacgcatttg catctcaact tgttttaaat cttcacctgc
     3541 tactaaaata tccatgttct catttatagc taaaggttca gctgatccat gaagatataa
     3601 atgattgatt gtattctctc ttttcgcttt actgaaagca tcaggaaatc gttttgatag
     3661 gttttgcgac ttatcttcat caatatacag caccttcata cctaaatttt cgacagcata
     3721 tccacctaga ttgtagattg ttgttgtttt acctgttcca cctttttcaa tgtttccagt
     3781 aatgattgac aacttctttt ccatgattaa acctccctta ttaatctttt gtaactccat
     3841 tatacactat aaacacgttt atgtatatat ttatatacat aaacgtgttt atatttatat
     3901 ttgcttttat attctacaac ataaacatat atatgtatat gtttatgttt atgtatatat
     3961 tcaaatatat aactattgca tttgtcatac aatcgtagta caatgtaatt gaaaaggagt
     4021 tgataaacta tgccagtaat aagtattcgt gtatctaaag aagaaaaaga ttttcttgaa
     4081 aaagttgctg aattcaacgg agataacatt tctgaattta tgcgtagtaa ttccctgcaa
     4141 acagcagaaa cgttagtaga ctttgccacc tacaagcaac taatgaaaga gcatgaggaa
     4201 aacgatcaaa gtatttccca cgaagatatg ctaaaagagt taggcttata agatgactta
     4261 ccaagtaaga tatgaacgtg aagctcaaaa agcgctaaaa aaaatggata agtttcaggc
     4321 aaaggttatc ctaaactgga tcgagaaaaa tcttgtaggc actgatgatc caagaaagca
     4381 tggtaaaggt ctaacagcta ataaatcagg ctactggcga tatagggtag gtgcataccg
     4441 tataatagct gatattagcg agcatgaagt gacaattctt atattgtcta ttggtcacag
     4501 aagtgaagtt tataagtaac agacaagctg ataaggcttg tctgtttttt tgttaaccca
     4561 acaaaatcac tgtttacgat cccactttac ctaaaaaaga acaatatcct cttgatatta
     4621 tacacaccag tgtgtataat ataagtgtaa acaagctaat taactttcaa aaggagcgat
     4681 tttttatggg atggtatggt gattattcat cagcaaatga agtgatcgaa gaaaagaaag
     4741 ggtacgccaa aaatggtttg gaaatcgtag gtaccaaagt aacgaagacc tatggaatta
     4801 tccttttcaa agatgcagaa ggaacctacg atattgacag cttcatcttt cgagatgaaa
     4861 tgtacaaacc aatctacttc ttaaatgaca gtcctgctta ctttaagaaa attccgaaaa
     4921 aatggttgaa attctttgtc ggtgatcaag aaaaaatggt taagctacaa ctagatagaa
     4981 ttaaagaaga aagcaataaa ccaaaacttg atgacttatt agttgcaggt aaaacttact
     5041 caatctggga tggatcgtat gtcgctgtct atcagtataa actcaaacgt agctttattt
     5101 ttcgactaca agatggatca ctcactcggt tcactcgact atcagcagga gatgttaagg
     5161 aagtaaccta aaacttaaat acataaacat aaacgtgtat acgtttatgt ttatgtatat
     5221 gtatatgaat ataaacgtat taggtacgaa cacttttaaa ttcttttctg ggctttgttt
     5281 ttagggtagg gctaaaaaaa acatcaccat ctgatccaaa acagctcaga aacgcattta
     5341 aacgtatctg ataccaatat tacagaagga gtgtatcgta tgattaacct atttctttta
     5401 gtgacagcta gtacattatt ctttactgga atatcactga taatttccta tttgatctat
     5461 aagaaagccg aaaatcacac aagcaaagca atcggactag ccgtttcatc ctttttaact
     5521 ttgctgactt tatttgttac tttatcgttt tcttttgttt agaacagctg atccattagc
     5581 acctttttct ctttacatta tacacactgt tgtgtataat gtatttgtaa acaagctaat
     5641 taacttttag gaaggagtga caagatgcta acagctactt tacagcaacc tactcacgtt
     5701 gctattgcag aaagttatac aattaatgca gggatcgtta aacagctgat caaggatcga
     5761 gaattcaaaa agtttaacag tcggcaagcg tataacagac agctaaagaa agctattcta
     5821 aagcacaaag attccttcat cgggaacttg gaaagtgtag atgttaaaac tgtttcaacg
     5881 gcattttcag ttgttgaagt gactattgac ggtcgcttga tttctctatt tgcttttgga
     5941 attaattgat agggagtgtg aactttggaa ggtaagactt ttgcagaaat ttggactgaa
     6001 tttattgagg taccaactcg cattgatccg ataacttctg aaaaggtatt ggatgcagac
     6061 ttctacaatc tcaaagcagg tactagcctt gaatgcgttt ggatgtattt agaagagcat
     6121 ttcaaaggcg taacgctcgg tcacgtctta taccacaatg gtgccgagtg ggatgcaatt
     6181 ggaaatatcc atttttctta atataagaaa ggagaacaca atatgaatta taccgatgaa
     6241 gaacaaatcg ctttggaata cgttttcaca aatgaagaag gggaggaaat tgaaaaagtc
     6301 ttgatcattt catcaaaaga cttggagaag gctttggctt atgaaccata cgaatcaacc
     6361 gtaactgtca ttgaattctt agatacctat acttctaatg atacttggga aattatgaat
     6421 atttgcaacg ctaataacat tcaatttgct gaaaaaacca ataaatcatt ttattagttt
     6481 tgcaataaaa catcctcttt ttctattgat tccatacaca acaatgtgta taatagattt
     6541 tgtaaaggga gggtagctat gcctaagaca gcaagagaaa ttgtaaaaat tctcaaatct
     6601 aacggcttca aaaagactgg acaaactggc agtcatgcaa aattttataa ttccaagact
     6661 aaaagaacgg tcgttgttcc gatccacaat ggtgatgtac cactaggaac agaagctaat
     6721 atctggaagc aagcagggct ttaacagcct tgccttccta tttggtatta ggcatactat
     6781 cctctcttac actagaaaag gagcgaggaa atgaagacaa aaaatatttt ttatccggca
     6841 atcttcgaga aagaaggaga agcttataac atcgttttcc ctgacttacc ggaaatatct
     6901 acctttggaa atagtcttga agaagcatat ttaaacgcta aggatgcttt aggattagcg
     6961 ctatatagtg taccagatgc agaatttcct aaaccatcag cagtggaaga attaactttg
     7021 aaagccaacc aagtggtagt aatcatccaa ctaaacatta aacttttccg aagaagctta
     7081 aatacaaaaa caattagaaa aaatgttagt gttccggaat ggttagttct tttagggaag
     7141 gagaaaaaca taaacttctc tcaactactt caaaaagcac tagaagaaga gttgcttgaa
     7201 aagtaaaaag gagaccgaac ctatgaaagc tacacactta aactatttct ctcgaacgtt
     7261 aatcaataat ctattaaaag atacaactgg cattagcgtt agtaccaaac actttgccaa
     7321 tgatctaatt atttttgagg ataataactt gcgaattgag atgtttattg aacaggcaac
     7381 ccctaactca atacctgaat tctgctatac gcacactgaa aaaatgaaac atgcaaaatc
     7441 agttaaagat gttgaagcat taagtgaatc actaacgaac gctttaaacc tcgttaagaa
     7501 ggcaaaattg ttatttgaat ttcaaaccga ctaccacaat gagttgcttg ctcaatctga
     7561 aattttagat taattgaaac aggagaagag aaagatgaaa caaacacaaa cagaaaccgt
     7621 taacgatacg cttgctgatc aattagcagg ttattataaa ttatttgaga tgggtaaagc
     7681 acagcgcttg atccactatc tttggaaata caaaattttg tttctttttt gggaatcaac
     7741 actaacattc tcagttcttt tttataagtc agatttttat aatcaacact tagttttagg
     7801 gagttttttg cttatctaca ccattttgat gcttaattta tatacctaca tcacacttga
     7861 agaacactta gtaattagcg atgcagggat cgagagaaac atcaaaaggg tatccttcaa
     7921 gatcacgtta aacaagccta ccgataaaaa gctctcagaa gcgttctgag agcttttgac
     7981 attttcttgt ataattttat cctaacacat tacaaagcac ctaattcgag cttatatcaa
     8041 gcaaatttgc tatttattac tcaatacata tctaaaaatt acttgatgga aaattagata
     8101 catggtaagc atgaaaagaa aatcaatttg agcagatgca gtacaataca tagcaattta
     8161 aaagctctca gaaggcttct gagagctttt aatatattct ttgataattt gtctttctta
     8221 aatccatacg tcctaaaaca agctgttttt gcttaatatg atcatctgag agacatcaaa
     8281 tcaacttttc tctcaacaaa tcattcaaag ctttcgacag attaatattc tcttcagccg
     8341 ctaaaacatt taaatagtgt tccacagtca ctgattttcg atcataattt tgcttgtttc
     8401 gagctttagc aactttcgta ttagctttta cgacaaataa attaacattc tcagatcgct
     8461 tgattcttgc atttaactct tgaacaggcg taggcgatgg aatttctttt tcttgcaact
     8521 ctaaaagcac caagtatcct tccaatactt cttgcgccat ctcaaaagca tcttctaaag
     8581 tttcaccatg tgtatataag tctggaaaat ctgggaattt aactgcatat tcatttcctt
     8641 tccattcgaa tactgcacaa aaaaagtatt tcatcgcatt gcacctctct aattaaaaaa
     8701 taatttttgc ttttttgttt caaaaaaaag aatcgcctac caaaaaaatt gataggcgat
     8761 cacaagctaa ttaacctcta aaggagatta ataccttgct cccaaggcac tatatatctt
     8821 agcatacgtc tgaagcatct tcaaacgagt aagatatatt ttgcattaac ctaatttttt
     8881 ttctctataa gttttttcat caaactcact agaaatatta aactggacag caaaattgta
     8941 atttctgcct tttctaactt tagcaaaatc aatattcagc attgattttt cattaatttc
     9001 ctcaactgat tcccttaaaa cacgtctatc aaaattcgat attgttttat actcattttc
     9061 tgttttcgta atctcacgaa ttttaactat ttcaatcagg ggattcttat atatttcagc
     9121 tttctcaaaa tctcctttat ttttggcgat aagatatttt ttataataca tcgccaaata
     9181 tttgtataac acgatgctat attttttttg aaaagatgct aagtcttcaa aaacaaagaa
     9241 cggatacttc tttttgattg agacaattaa gggaattaca tcttctgaaa aactgaaaga
     9301 tacactatgc tcttctaaat cccaatgaat ccgatcaata gcagatacag tagtatacgt
     9361 cttatatttt ttgtctagat tcgaaatttc ataaactgct tgtctttgca aagtgttaac
     9421 tatggttttt agttccgttt caagattttg atcaacatcc atgtaattaa taatcgcttt
     9481 aacaggtaaa tctactacgc ctgttttctt tagctttgtg ggtgagatat aactgaacag
     9541 caattgataa agttcatacg actttaaatc cattttcatg tcaattgttg ctaagaactc
     9601 ctcattgtac tttgtcagtt catctttttt caaaagtttt tccttccttc acgcattttt
     9661 tcagttgtta atcataaagg ttcacataag gcaaataact cgtaattgat ttttcgagat
     9721 atttaacagt attttttatt gtgtaatcct ccttatgagt agcaacgtaa gataggtgat
     9781 tgacaactga ttcaaatcct ctatctttgg ataaacgatc ataaagcgga taaactttat
     9841 tctgaagtcg gctcataagt ttaagatcgc ataagtcggc agaactgatc aaaaagtttt
     9901 tgattagtaa tttagtgtat ttgctattca tagctttcaa gtacgattga ttcattttct
     9961 ctgttttatc ttctgcgcct tttaaataag cagggtctct ttgttcttgc ttataaggta
    10021 ccggaactga catggtacgt gtaagcaaga actttatgtt tgtataagta tgacccttct
    10081 tgataagctc atagtcaatt ttaaaatcag tgtatttatt cagttgggca atcggttctt
    10141 taattacacg tctttcaaag tctcgaaaag ctttatattt tttctcaata ctaaatttct
    10201 ttcgtaaatc tgcaatggag aaaacaggat tttttaatct atcaagtgat tttgtactcc
    10261 atgttaagtt atctctattt gcttcataca gattaaaatc tttagtgaat aattgaaaaa
    10321 aactgatcgc atacttgctt gtcaagttaa ctacttgatc aaatgtatat tgcgtaaaat
    10381 gattttctaa gttcgttaat aaaggcataa ttgaactact aaatttaata gatactgcat
    10441 cagaaaaatc attgaattca gtaatttcta gcggtgagat gactttgtat tcagtcttac
    10501 cgttaggttt agttctttga attttaatga cagattgctg atgcaatttt tcaataactt
    10561 gttttaatct tgaatgtttt gaagaagatg aactagaaga catcaacgca aataaagcct
    10621 ttttagatac tttgacggta ttattaaaag gcggattttt agggtcaaga tgtcctatag
    10681 cgtaaagata aattttttgt ggcatagcat ccatttcaac aatgctatct acaagttgat
    10741 tcgataaagt aacagttaaa tcaaactttt ttagattgct ttcggtagtt tcaaatttcg
    10801 ggtttacatt cttttctgtt attttcatac gattgcctct tctttctaag cttggtacga
    10861 gcttaaaaac ttggaactgg tacgattcca ttgaataagt atagacctgt tttcgatacg
    10921 ttacaataag aaatgtatcg taaatttata acataactca atgtcagtaa aacacatttt
    10981 attttttcca atcaaatttc attgaaaata cagcagaaat cgctatttta gaacattttc
    11041 aaaataaaaa tccttttcaa taaaatacga aattataaaa attaccaaaa ttaaaaaaca
    11101 aatatttttc tagtttttag aaataaattc acatactttt tctttttttg gatatattcg
    11161 gaataccaca tcgctttttg tccacgtttg catcgctttt tgtccacatt cacatcgctt
    11221 tttgtccatg ttcgcatcgc tttatgtcca ccctaaaatc tatttaagat tgatataaag
    11281 cctttttgga ggttgctaaa gttttaaaga ttaaaatata tattataaat ataaataaaa
    11341 ggggaagctg aagcttcctc cttttccttt ttttcaatta ttcaaagtta cacaagcaaa
    11401 ataaactcct agatagtgtt agtttttttt aagatttttt atttttaaga ttttgaaata
    11461 agagaggagt aatcctcctt tattaaatat ctgttaatat atctgttaaa tatctgttaa
    11521 tatatctgtt aggggaggct taatctcttg ggagagtaag tattaaaggg gtataaaggt
    11581 aacacttttg gacagtaaag gtaacaaaag tggacagtaa gggtaacagt tttggacagc
    11641 aaaggtaaca aaagtggaca tttgaggtat cacactagga taagacgtta ccttcggttt
    11701 tcttgatttg gaaggcgaaa aaatagaaaa ttagattagt aacaaatctt tgttgactgt
    11761 tacctttagt tgtgatttaa tttaggtaac aagcagaaaa aattgtttcc tttaattagt
    11821 accagattaa catgattaaa ggagcgcaaa aagaagattg gagagtaatc aatgtctcaa
    11881 gaagttaccg aaaagattat tgatagtgat gtagatttag tatcagaaca aacaaatgca
    11941 attgtgcgtt ataacaatgg tctgaataca acagctttgc gaggatttaa gcctgttgaa
    12001 atggatgtgt tttgggctat tttatcaaaa atgaaacgtc aaggaacagc agaagtaact
    12061 tttgatttca acctctttcg ttatttggtc aaatacgaca gaagagaaaa ggacagtttt
    12121 tattctgtgt taaaagattt gagtgacaaa ctaggtaatt taacctaccg ttttgaagat
    12181 tcagacgtct atgaacagct gtggcttttt caaaaattcc ggatagataa acagaaggaa
    12241 acagttacaa ttaaggcaaa tgaggaattt gaatttatct taaactcaat tggcaaaaac
    12301 tttactcgtt ttgaacttga taattttaca aaactaagaa gtagctacac aaaagaattg
    12361 tatcgtcatc tcatgcaata cagaaataaa gagtggcgaa atggttattg ggtagtcaaa
    12421 gttgaagatt ttagaagagc attaagtata ccttcaagtt atagaatgac aaatattgat
    12481 aaaagaatac tagagcaggc gaaagaagaa tttttagctc ctgatgaaaa cggaatacag
    12541 gtatttgaaa cttttgatta tgaaaaaatc tatgcaaaga aaggaagaag agttgatcgg
    12601 ctcagattca cttttagtga gccagaatca aacttgccaa ccatttcaat gcataattgg
    12661 ttagaagaag attgagaaga agcaggttgt aattttaata caacctgctt ctttaaaaaa
    12721 gtatgggtaa cacttttgga caataaatgc aagaagtttt tttgtaagca agtttcagtc
    12781 taaataatat ttgaaagaat ggctaatcta tacaaatttt aacaactatg ggtaacactt
    12841 ttggacaagt caagaaaaaa aacaattttt cttaatttgt tgatcgacta aaggtaacac
    12901 ttttggacaa cacagaataa aaagtccggt gatatcagtt ttggtaggaa acatcttaat
    12961 atctttgtga gattgaaggt aacatttttg gacaagtcaa ctatttgtta aaagttaata
    13021 tagagtaatt agataagttt tttcttttcg tctgataaat ggataatatt agttatttta
    13081 ttgctaatta gtctttcatg atgactgaaa tagctaaaat aaatatctcg ttggataatt
    13141 gctgatactt taagggatat ctaaatttgc tgattgattt atttatgtta ttatgatatt
    13201 ataataacat aaatgtgtta cgcccgaacc tcattttaaa aaaatgaagg ggtgagacaa
    13261 tgaatgctat taattggcat gaatttgtta actatatgga gagcttacta gatgatctaa
    13321 aaagttttag tattgctgat gcagtaaagc atgggttacc agttagtaca acgatgaaat
    13381 tacgaggatt gaaaattcca gatgaaatta ttaagctgag aactaaaaca attattgaaa
    13441 ccgcaaaagt cgttgacaat atgaaccatg aactaaaagg caagaaaaca acttacgaga
    13501 aagattttga acgtttttta aaatctgtta ttggaaaata tctaggtaaa aaatctttca
    13561 gtgaattgag agatgaagag attgtaccta ctccaatggt tagcaaaatc cgaaaaaaca
    13621 aagccgaggg gaattactat cttggtacaa tagcaaaaat cattgattat ttggaaaata
    13681 aagagcaaaa gaaaggaacg aagaaaggaa gataattttt gtgttaaata ttaatgggga
    13741 agaatatacc gaaatgattc aaaatcccga acctagaatt accgaaaaaa cgtctgattg
    13801 tttgctcttt tatgaaaggg ggcagacaaa ctaatggaaa aaaacgtcaa ttcaacatct
    13861 accaaagatc aatacagtat tattggaggg ttgcttggtg ataaggataa gagaacacgt
    13921 cacggattac gcattatgct tgcaactttt ctaggagtgc tttttttatt aacaggcttg
    13981 ggtagctaca tttatcaatt aggaaactat aattattatc tcgtgaacaa tacaacacct
    14041 ataggctctt cattggagtt tgctcgatca ggagcagaga tcactatttc agatgtgtgg
    14101 acagatcaaa acagagatgt aacagtagta aaactcggtt actcgcaaaa cgctagaaag
    14161 ttgttatcta cgcaaggaaa aaactacaac ttatatatgg caacgaaaga aaaagatcgt
    14221 cctaaaaagc ttgaaatgag ctatgggatg ttaagtactg atggggatgc tttcttattc
    14281 ctaaaaggta aattagatga aagagcttat caagttttta ttgcaaacca agtatcacta
    14341 atttctggtg atgatacagc tgacgatgat accaaagtca atgtgaatga agcagattca
    14401 gttacaaaag cactttctca atattctatg aatgatgtta ataatgaagg agtaatttct
    14461 tttgggaaaa aagcaaaacc tgttaatact cctgataata tcaatttcag aataaatcca
    14521 tattcagatt caactaatgt ctatcaagga agctttctta ccaaagatgg cgatattgat
    14581 tattcaaaag ttgtagcagt tacaagtaag aataatgttg taagtaagtt agaagatcag
    14641 ttaaatgctt caaatactaa catcaagaaa attgaagcta caaaagctga gtatctggaa
    14701 cgtttacaac aaaatcctga cgataaagat tcgcaagaaa gtattcgtca actagacagt
    14761 cgaatggaga ccgaaattaa agcaatcaat gatacacaaa aattgattga tcaatacaaa
    14821 aatgctgact ttaacaaaga ttcttttgga gacatgaaga cgacatttaa gtacctaact
    14881 ctgactgact agaagtaaat tattcttatt ttagaggaag tgaacacaaa cagatggaaa
    14941 atagtaccga gttttttaag aatattcaaa ctgaatttaa tcatgctctc acaccaatga
    15001 aaccaccgaa acataagtta gtgggacgtg atgacgaact ccatgagttg tcactgatct
    15061 tgaatagacc ggaaacaccg caagctatct tgttaggtga agccggaaca ggtaaaacag
    15121 cacttgcaag tgaatgggct gaaagacagc gacaagatgg aaagaaagta caagtataca
    15181 aattatcaat cggacaattg cgtgttgggg gagatgatgt cctccaaaca cgtttagaaa
    15241 cattaattcc aagattgaaa gaatatgagc gaattttaaa atctgaggac aaagaaaatg
    15301 aatttgtcct cttcattgac gagtttcata aagttgttag ctcttttggt agtggcaata
    15361 aaattggggg tgattcgcta aaagaaccgt tagcaaatga atcacttcaa ttgattaccg
    15421 ctactacaag aaaagaatat gaaaactata tcaaaagaga tgatgcctta gggagacggt
    15481 tacaaccgtt agaattgaat gaactaaaaa gcgatgttgt cgtcgatatt ttgagacaat
    15541 ggctaaatac atcctctgtt aaagatgatc ctcaggcttc ttttaaatta tcgcaaagga
    15601 ttcctgatga attacttcaa gaaatcgtac aagcaaacaa gctttatcgt gaggatttgt
    15661 ccgaaccggc taaatcaatt gccgtaatgg aggatttgat tgcaaaagat cgagtagacg
    15721 gaaatgctta taatcgagaa agtctattgc aaatatttgg taagtataat attcagctag
    15781 attctgattt tgatatggaa aaacttcaag aggaattaaa gtccataaaa gggcaaccgt
    15841 tagcagttta tactataaca atgttgttta aacgtatgaa gcttggattt gatcgtttta
    15901 aaggtcgaaa gcaaccaaaa gcaactgctc tgtttacagg atcaacaggg gtcggtaaaa
    15961 ctgcaatggc taaagccgtg gcaagaggac tttatggcga tgaaaataaa gttctcttag
    16021 ttaatatgcc ggactacaaa acaaaagatg atgcaatgcg ttttagacga ctcataggta
    16081 ctcatgtaag gcatgatcct tctgcgatta ttgtgtgtga tgaatatgag aaagctggtg
    16141 tagaaacact cgatgccttt ttatcaattt tagatgaagg taggataagt tttactgaaa
    16201 gaggatggga tgatcgagaa gttcacgaca ctgtttcatt gtcaaataca attttatttg
    16261 cgacaacaaa tgcaggtgcg gagatgttcg attcgttaaa cagatatagc tcactatctc
    16321 gtgttacaaa caaggaaaat tctaatgaat atagcactga actaaaaaga gaataccgaa
    16381 aagtcgcatc agggatcaaa caagccttga ttggtgaagg tctaaagcct gaagttatta
    16441 accgatttgg acgaatagtt ccattttggg ctttatccga acaaactctt ctggaaattg
    16501 cttataatga aatgttgaag agtgctgaat tagtacgaaa acatttaaat atagatttac
    16561 gtcttccacc taggaaagaa agagtatatg gtcaaaattt ttatgcagac gatctctcca
    16621 tgcacttagt ttttgacatg gcttcacaag aggattctaa atcagggggt gcaaggaata
    16681 tcttgcattt agttgaatct gagtttgaag aagcgattac tgaagcaata attacttatc
    16741 caaacgcaaa aatattcgat gttaagttga gtgaagccgg agcgtttgaa ggaaaaggtg
    16801 attctaatgc tgaaattatt gcaaagcctt ccgttgctga aagtaaagaa aacaagattg
    16861 tattttcgat tggaggggcg tagctatgaa attcaagaaa tatttactac aaaaccttag
    16921 aataaaacga tgggtcgtaa gaggtctagt tattggagga gtattagcag ttactttagg
    16981 cgctcctttt ggaatgaatg atccaactcc tgacaatcaa gatagcaaac aagaaacagt
    17041 tataaataag aacgttgcat atgcggaaag taaaccggca gaacaaggaa tgtggacact
    17101 atatgctcgt atcctaatgg gagataaaga agttaatgat agtgccgcta aaactggaca
    17161 aatgaatttg ttaggacaag gtggtatcag tgccgatttc ccttattctg acatttctaa
    17221 acaaggtaat tcgattgcag ggggtaatgc aggtactcaa ctagcatcta ctttagcaac
    17281 ttatagttac tacggatata ttgaaacagt ctcaggaaat gctattgcca gtcaagcatc
    17341 atccattcta ggaagtattg gtaggacgat tggtagtttt atcggatcaa ttagtttaat
    17401 cctttctaat attgctgaat ccataaatga actccttgga aaatttttca tgagtatgaa
    17461 tatattttct cttatggggt ggggagaagg tggatcggat gtatctaatc cggtatctaa
    17521 agcattaact agcttgctta acaatttggg attaaataat gaattattta aagcgatagc
    17581 aagcttatct atgctctttt tcacagcaat actaggttat caaggaatga aattattgtc
    17641 gaaagatggt gttaactcga agggattatc accagtactt gaaaaatgga ttgttcgtat
    17701 ttttgtattg tttatattct taccagtggc aggatcgcta ttgggagttt atatcaacgg
    17761 catccagtca gcaaaaaaag atggagatat accaacaaat gttgtttctg actacatcat
    17821 taacacaaga ctttgggctt ctactcaaaa tcttgctcca tcatctgata ctggtggtga
    17881 tgtacctgac ggaaagccta catccgaaat caagttcgtt gatacagatt atgaaccatc
    17941 taaaaaatct gcaatgataa gagcgatcaa tactagcagt tatgccagac aaaataatga
    18001 tctagattct aattcagtcg gtatggctct attaaaagaa tggtcatcga atgatacttt
    18061 caatattaat acttatgccg gtgatgttaa tgtcgccgaa aatgatgctt atatgggaag
    18121 agcgcctgct gtttggacag gctataattt aggggatgga aaagaaggaa gtgcaattaa
    18181 gaaaccaacc aatttaaatg gatatatttg gtcagcaaat caaaatgtga cagacgacaa
    18241 tcgcaagcct gatggaaaag gaacagcgca taactttgat gctactcaac cttggggcgt
    18301 tgctaacaat gcaactttta gcacacagtc ggtagcttta ttgctccaat cggcacttga
    18361 tacatcggga gctaagtttt atgcgtataa tatcgctcct tcaggaattc aaggcagtgc
    18421 taagaatatg tcaacagtaa aaacagaatg gcgtacagca tccttagttg gtcatggagc
    18481 tatgggtaag tttggctcgt ggctagcgat gaacgctgaa tcaatcgttt tagcagttct
    18541 ttacttagca tgtgcttggg cgatgttcaa tgtgaatatc gtgaaagcat ttaaacgtga
    18601 atttattcgg atttgggaag tagttctaac aggatcactt gcatcaggag caggtgtgat
    18661 ggcaatttca ttaggtgttg ttacaacatc tctgatcgga ctacaattac ctatctttct
    18721 gattactttt attcgtgcat ttggtgatac tgctgtggga ttcattccta atacattagt
    18781 tggcgatggc gtagcaaata ttgttcgatc tgctatgatt ttaggtgtag gatatctttt
    18841 agtctttaaa aaaggcgaaa aagtaaataa tttgattaat atgcttattg gcttaccact
    18901 tgaagtagcg tttgggtttg atgaacgtgc taagttatta tttggtaata gaaatgatgc
    18961 tgtgcgagtt gtggacggcg tttctaaagc aatcaagaaa agccaaggag ctaaaaatcc
    19021 ctattatgat ggtggcaaca gctcagtaaa agatatggtt gcaggaggtc ttggagcagt
    19081 tggagcgcaa aaaatggctg aacgtttgaa caatatggga gatagcaatg ctgatgctaa
    19141 gtccattgct gataaagtaa ttcctgaaac accagaagga atgtcattca acggtggcgg
    19201 agttggttcc acaatcaata atacctcttt caaagattta gttcgtcaag atcaactacg
    19261 taataactcc caacaatata ttgatcattt aggacgtatg gttgatgaaa acggaaactt
    19321 tgtcgatgaa aatggtgaaa tattaaaaga tagtaaatac ggtaaagatg ctaaaggcaa
    19381 ctttattgac gaaaaagggc gtttggtcga tgaagatgga aaccttatag atactaatgg
    19441 taaaccattg gaagatagtg aagatggtaa tattggtatg gatagcgaag gacgtttaat
    19501 tgatgaaaaa ggtcgcttag tcgatgaaga tggaaatctt gttgacgaaa atggacgcag
    19561 gttgctaaat ggtgattttg gtcatgatag taacggacgt ttagttgacg aaaaaggtcg
    19621 tttagttgat gaagatggaa atcttgttga tgagtttggt aataaacttg gtaaacacag
    19681 cgatggatat aatgtgaatg gagatttagt cgatgaggat gggaatatca ttgagacgag
    19741 agatgcgctt gtggcagatc gaaatagaga actcgaacaa caacggcata cgccaattgc
    19801 tgttgcaatt cctgagaatg aagaagacga gattacttta gatcaaactc atcaagaact
    19861 gaggaatgaa atgagtggtg aggacgtgtc agacagagtg catcgggaat taggagatgg
    19921 agcatctggt gaaagtcagt caagagagct taacagcaac tttgataata gtgctattcg
    19981 gaatgacaca gtacttaaag acagagaatc gctaacaaaa gaaacggata aagaacaatc
    20041 agatgtgaag aaaaagttca ttcctactcc taatgttaat caaacagata aatatgaaac
    20101 aaatattgga tcaggaatgt cgaaagacaa tcaatcaatt agagaacctg aactgacaaa
    20161 taaaaataat agaacaaata attctaaatc tacttcagat aattcaggga aatctaatca
    20221 atcaaccaag aaaacatcaa cagctaaatc agccgttgaa tatctatcaa aaactggtaa
    20281 aaatgtttac acctcagcag ataaagcttt agcaaaagct ccaaaaggta gtctagcagg
    20341 gataacctat gaagctaaaa acgaagctat taaaataggt aaaggaacgg ctaaggttgc
    20401 caaggctact gctacaggag catccaaaac tggtaaagta gtgaagaaat ctaccatgtc
    20461 agtcaaggac tatattgtaa aatacggtaa tcaaaaatca actgaaaata agtctgaaga
    20521 cgagtaaaaa gaaggagcat tgatttgctc cttcttttta ctttgatttc taactgaaga
    20581 tgtcaaggaa aatttctatt tatgcaaaaa aattatttta aattgtctat aaatgaatta
    20641 aatatttact gttcaaagtt gtataattaa aaagggctta tgctggtaaa gaacagaatt
    20701 attatctaaa caaaaacttg ataaaccagc atttttagcc aataaaaaaa gtgaaataat
    20761 ttaatggaaa atctctttta atcatcgaa
//
LOCUS       DAXWLF010000034        20573 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXWLF010000034 DAXWLF010000000
VERSION     DAXWLF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.034"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            172..900
                     /gene="purC"
                     /locus_tag="ACGE4U_000971"
     CDS             172..900
                     /gene="purC"
                     /locus_tag="ACGE4U_000971"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFF8393153.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            900..1154
                     /gene="purS"
                     /locus_tag="ACGE4U_000972"
     CDS             900..1154
                     /gene="purS"
                     /locus_tag="ACGE4U_000972"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFF8393154.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1156..1827
                     /gene="purQ"
                     /locus_tag="ACGE4U_000973"
     CDS             1156..1827
                     /gene="purQ"
                     /locus_tag="ACGE4U_000973"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFF8393155.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1828..4050
                     /gene="purL"
                     /locus_tag="ACGE4U_000974"
     CDS             1828..4050
                     /gene="purL"
                     /locus_tag="ACGE4U_000974"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFF8393156.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4035..5474
                     /gene="purF"
                     /locus_tag="ACGE4U_000975"
     CDS             4035..5474
                     /gene="purF"
                     /locus_tag="ACGE4U_000975"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFF8393157.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5497..6549
                     /gene="purM"
                     /locus_tag="ACGE4U_000976"
     CDS             5497..6549
                     /gene="purM"
                     /locus_tag="ACGE4U_000976"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFF8393158.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6546..7124
                     /gene="purN"
                     /locus_tag="ACGE4U_000977"
     CDS             6546..7124
                     /gene="purN"
                     /locus_tag="ACGE4U_000977"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFF8393159.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7121..8656
                     /gene="purH"
                     /locus_tag="ACGE4U_000978"
     CDS             7121..8656
                     /gene="purH"
                     /locus_tag="ACGE4U_000978"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFF8393160.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8672..9946
                     /gene="purD"
                     /locus_tag="ACGE4U_000979"
     CDS             8672..9946
                     /gene="purD"
                     /locus_tag="ACGE4U_000979"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFF8393161.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10068..10982
                     /locus_tag="ACGE4U_000980"
     CDS             10068..10982
                     /locus_tag="ACGE4U_000980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFF8393162.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11115..12680)
                     /locus_tag="ACGE4U_000981"
     CDS             complement(11115..12680)
                     /locus_tag="ACGE4U_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8393163.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12673..14124)
                     /locus_tag="ACGE4U_000982"
     CDS             complement(12673..14124)
                     /locus_tag="ACGE4U_000982"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8393164.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14138..15154)
                     /locus_tag="ACGE4U_000983"
     CDS             complement(14138..15154)
                     /locus_tag="ACGE4U_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8393165.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15374..16462
                     /gene="chvE"
                     /locus_tag="ACGE4U_000984"
     CDS             15374..16462
                     /gene="chvE"
                     /locus_tag="ACGE4U_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8393166.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16516..18042
                     /gene="mmsA"
                     /locus_tag="ACGE4U_000985"
     CDS             16516..18042
                     /gene="mmsA"
                     /locus_tag="ACGE4U_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8393167.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18044..19249
                     /gene="mmsB"
                     /locus_tag="ACGE4U_000986"
     CDS             18044..19249
                     /gene="mmsB"
                     /locus_tag="ACGE4U_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFF8393168.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAVRLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19506..20483
                     /gene="guaC"
                     /locus_tag="ACGE4U_000987"
     CDS             19506..20483
                     /gene="guaC"
                     /locus_tag="ACGE4U_000987"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFF8393169.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT         6606 a         3480 c         4701 g         5786 t
ORIGIN      
        1 gatggtataa gattttcgtt tctgataaaa cacgaacatt aaaagaatct aatgccttaa
       61 atgtacatat ttttgttgat ttataaaaag atgattgcta aaatttaaag tgaaaaaacg
      121 aacaaaaaac gatgtatttg cttttaatgt aaataaaaag gagagacgta catggagaag
      181 aaagaacttt tgtatgaagg aaaagccaaa aaaatatatc ttacttctga tgagaacttg
      241 atttggatag aatatttaga tcaagcaact gcgttgaatg gcgcgaggaa agaccagata
      301 agtggaaaag gagaattgaa taatcaaatc acaagtgcaa ttttccgtta tctgactcaa
      361 agaggaataa aaaatcattt tattgaacaa ttgtctaaaa ccgaacaatt agcaaaaaaa
      421 gtacaaatta tccctctgga agtggttatc cgaaatattg cagcaggaag tttctcaaaa
      481 cgtctaggtg ttcccgaagg taaagaattc atcaaaccaa tcattgaatt ctactataag
      541 gatgaccagt tggatgatcc atttatcaat gaagagcata tctatttctt acaacttgca
      601 aagccggaag aaatcagtga gctgaaagcc caagcgttgg ctattaatga aattttgacc
      661 gaattatttg ctaaggtcgg tttggtttta gtagatttca agctagaatt tggccggtcg
      721 aatggacaaa ttttgcttgc ggatgaagta acaccggata cttgccgatt atgggatgaa
      781 cgtacgaatg aacatatgga caaagacgtt taccgcagag atctaggtga tttgatccca
      841 gtctatcaag aagtatataa ccgtctatcg gaactaactg aagaaaagga gcgagtatga
      901 tgtatttcgt aaaagtatat gtaacctata aagattcagt gttagatcct caaggggagg
      961 cagtaaaaaa agcagtccat cgtatgggat atgaaacaat cgaagatatc cgaataggaa
     1021 aatactttga gatacaactt gctgatgcag aagaaccaat cgatacagtg attgaaacga
     1081 tttgtgacaa gctgcttgca aatgtaaata tggaaacata ccgatatgaa atcaaaaaaa
     1141 tggaggaagt ataacatgaa atttgcggtc atcgtttttc caggttcaaa ttgtgacgca
     1201 gatatgttat gggcagttcg cgagatcatg ggcgctgatg ccgaatttgt acggcatgat
     1261 gagtcctctt tagaaggatt tgatggggtt ttactgccgg gaggattctc ctatggtgac
     1321 tatttgagat gcggtgcgat tgctcggttt tctacaatca tggaagaagt gattcgtttt
     1381 gctgaagaag ggaaaccagt attcggtaca tgcaatggat tccagatatt gacagaagca
     1441 ggacttttgc caggtgtact gagacgaaat gagtctttgc attttatttg caaaactgta
     1501 ccattgaaag tcaaccccaa gacggccttt acttcagagt atgaagccga cgaaataatc
     1561 tatcttccag ttgctcatgg ggaaggaaat tatttctgtg acgaagaaac acttcaagaa
     1621 ctaaaaaaaa ataatcagat tgcctttact tataatgaca acatcaacgg cagcgtggaa
     1681 aacattgcag ggattacgaa caaagcaggg aatgtgttag gaatgatgcc tcatccagaa
     1741 cgtgcagtag aagacttgct aggatcagaa gatgggaaac ggttctttgc ttccatttta
     1801 aaaaacttcg gaaaggtgac cgtatagatg aaaacattga aagaaccgac agctgaagag
     1861 atcaagaaaa aaagaatcta tgccgaatgg ggattgactg atgaagaata tcgaatgatc
     1921 gaagaagata tcttaggaag aatgcctaat tatacggaaa ctggactgtt ttctgttatg
     1981 tggagcgaac attgttccta taaaaattct aaaccggttc tacgcaaatt tcctactagt
     2041 ggacctcatg tgttgcaagg ccctggagaa ggcgcaggca tcgtcgacat cggtgatgga
     2101 ctagcagttg tcttcaaagc agaaagtcac aatcatcctt cagccgtgga gccttatgaa
     2161 ggagctgcga caggagttgg cggaatcatc agagatattt tcagcatggg ggctcgtcca
     2221 attgcgttgc tagattcatt gaggttcggc gaattagata atccaagaac gaaatattta
     2281 ctagaagaag tagttgcagg tatcggcgga tacggcaatt gtatcggcat accgactgta
     2341 ggcggcgaaa ttgcatttga tccttgttat gaaggaaacc cattagtcaa tgcaatgtgc
     2401 gtaggtttga ttgaacacaa acatatccag aaagggcaag ccgctggcgt cggtaattcg
     2461 atcatgtatg taggtgcaaa aacaggtcgc gacggtatcc acggagcaac ttttgcctca
     2521 gaagaattta atcaagaaga agaacagcaa cgttctgctg tacaagtagg cgatcctttt
     2581 atggaaaagc tgctgcttga agcgtgtttg gaattaatct tgcatcattc ggattctcta
     2641 ataggtatac aggatatggg agcagcagga cttgtttcat ctagtgcgga aatggcttca
     2701 aaagctggct caggacttat attgacatta gatgaagtcc cacaaagaga aactgggatg
     2761 acaccttatg aaatgatgtt atcggaatca caagaacgga tgttgatttg tgttaaatca
     2821 ggagaggaag aaaaaatcca agaactattt cagaagtatg atctagatgc agtcactatc
     2881 ggtaaagtaa cagacgacgg gcaatatcgg ctatatcatc aaggagaaga agtagcaaat
     2941 ctgccagtcg atgcattagc tgaagatgca ccagtttatc ataaagaaat gaaagaacca
     3001 gctcgaatcg tagaatttca gcagttagct ccttatcagc cagttatcga agaacctgga
     3061 caagtattgc ttgatctgct gcagcaaccg acgattgctt caaaacgttc aatctacgaa
     3121 acttatgatt cacaagtaca aaccaataca gttgtccgtc caggaagcga tgctgctgtg
     3181 atgagggtta gagggacgaa taaagcgtta gctatgacca cagactgcaa cgcgcgctat
     3241 ctgtatttgg atccaaaaat cggaggacaa atagcagtgg cagaagctgc tagaaatatt
     3301 attgctagcg gtggtaagcc cttggcgatc accgactgcc tgaactatgg ttctcctgat
     3361 aaaccagaag ttttttggga attatcgact tcagctgacg gaattgctgc tgcgtgtgaa
     3421 acgttaggaa ctcctgttat ttcaggaaat gtctctttat ataatgaaac ggatggtcaa
     3481 gcaatctatc caacaccaat gattggtatg gtcggattga ttgaggatca taaacatatc
     3541 actactcagg aattcaaaaa atcaggggat ctaatctata tattaggtaa gacgtttgca
     3601 gattttgatg gcagtgaatt acaaaagatg cagcttggaa gaatcgaagg tgtgatccga
     3661 aactttgatc tatccattga aaagagaaac caagagttag tattgacagc catccaaaac
     3721 ggattgattg aaagtgctca tgactgttca gaaggtggct tggcaattgc actggcagaa
     3781 tcagctttca aacatcagct agggatatct gtacagtttg aattgtcctc tgctcaatta
     3841 tttgcagaaa ctcaatcaag atttgtgttg acagtcgcac cggaaaataa gacacgattc
     3901 gaagaaatga tgggagatgc tgctgtttta gcaggtaagg tgacggatga agcgataatc
     3961 gaaatttcag caactgacgg tcagatcaaa atagaaactg cagtagcaag aaagtgctgg
     4021 gaggatgcaa tcgtatgtct tatgaagtga agagtttaaa tgaagaatgc gggatcttcg
     4081 gtgtttgggg tcatccagac gccgctagtg tggcatactt cgggctccat agtcttcagc
     4141 atcgaggaca agaaggtgca ggtatcgtgt ccaatgatcg aggaaagctt aaggggcatc
     4201 gagatttggg gctgctttct gaggtattca aagatcaaag aaaattgcag cagttaacag
     4261 gagaagctgc aatcggacat gtacgttatg caacagcagg caatggaagt gtggacaata
     4321 ttcagccttt tctattcaaa tttcatgatc agtcgatcgg cttagcgcat aatggtaact
     4381 tgatcaatgc tcgttccctt agaaaagagt tagaggaaaa tggggcgatc ttccattcta
     4441 actccgatac ggagatattg atgcatttga tccgacgaag tgaagaaaca gattttttaa
     4501 ataaattaaa aagtgctttg aatgaagtca aaggtggctt tgcctattta ttaatgacag
     4561 aaacggcaat gattgccgct ttggacccaa atggttttcg tccattagca atcggacaaa
     4621 tgaaaaacgg agcatacgtg attgcctcgg aaacttgtgc attagaagtt attggggcac
     4681 gttttataag agatgtccag cctggtgaga tagtgatcat caatgacgaa ggaatccaga
     4741 ttgattgctt tacgaaggac acgcagcttt ctatttgttc gatggaatat atctattttg
     4801 ccagaccaga ttcaaacatc gcagggatca atgtgcatac tgcacggaaa aatatgggac
     4861 ggaatttagc gaaagaatcg ccagtaaaag cagatatggt cgttggcgta cctaattctt
     4921 ctttgtcagc cgcaagcggt tatgcggaag ctagtggaat cccttatgaa atcggattag
     4981 taaaaaatca atacattgcc cgaacattta tccagccaac gcctgagcta agagagcaag
     5041 gtgttcgaat gaaattatct gctgtacgag gagtagtgga aggtaaaaag gtgattatgg
     5101 tcgatgattc aattgttcgg ggtacgacaa gcagacggat cgtccatctt ttgaaagaag
     5161 caggggcaaa agaagtccat gtgaggattg cttctcctcc tttgaaatat ccatgttttt
     5221 acgggattga tattcaaacg cgtaaagaac tcattgcggc aaatcacacg attgatgaaa
     5281 tcaaagaatg tatcggtgca gattctttag gttttttaag tgaagagggt ctgatcgaat
     5341 cgatcggtct ggaaaaagat gctccgtatt caggattgtg tatggcttat ttcaacggag
     5401 attatccgac gccgttatat gactatgaag aaaaatatca ggaatcacta aaagaaaaag
     5461 tatcgttctt ttaaaagcag accaagaagg gagtatatga attcaatggc aaatgcatac
     5521 gcaaaagctg gcgtagatgt cgaagctgga tatgaagtag tagaacggat caagaagcat
     5581 gtcaaaaaaa cggaacgttt aggtatgatg ggcgctatag gaggattcgg cggttgtttt
     5641 gatttatcgg tctttcaatt aaaagagcca gtccttgtct cggggaccga tggcgtgggg
     5701 acgaaattag cagtggcaat cgaagcagac aaacacgata caatcgggat cgactgcgta
     5761 gcgatgtgtg tcaatgatat cttagcacaa ggcgcagagc cgttatattt cttagactac
     5821 ttagcaacgg ggaaaaatga tccgttacgt ttggaaaatg tagtaaaagg tgtagcagat
     5881 ggatgtgttc aaggaaatat ggcgctgatt ggcggagaaa cagctgaaat gcctggtatg
     5941 tactcaggta atgattatga tctagccgga tttgctgtag ggatagctga aaaatctcaa
     6001 ttattgacag gagaaacgat caacccagga gatgtgttgc ttggaatagc atctagcggg
     6061 attcattcaa atgggtactc tcttgttcgc aaaattttct ttgagcagca gcagttttct
     6121 ttatcatcaa aactgccaga attgaaaaaa acacttggcg aagaattgct tgagccaacg
     6181 aaaatctatg tttcttcctt gcttccgctg atcaaagaaa agaaaataaa aggagctgca
     6241 catatcactg gtggagggtt tgtagaaaat atcccgcgaa tgttaccaga atctatggct
     6301 gcacaaatca aacttggctc atggccgatt cttccaatat tcggctgttt acaaaaatat
     6361 ggtgaaattc ctgagatgga gatgtatgaa atcttcaata tgggcatcgg tatggtcctg
     6421 gcagtccaag aatcgaaagt aaaagaaata gtacacgaaa ttgagcaagc tggcgaaaaa
     6481 gtttatgaaa tcgggaaagt gatcgccaaa gaaaaacatc gtgtggtgtt tataggagaa
     6541 gaacgatgag aatcgctgtt tttgcttctg gaaacggaag caactttcaa gcgctagccg
     6601 attatctatc gaaaaaagga ttggaatctt cgattgactg gttgttttgt gatcagcctg
     6661 aagcatatgt tttgaaacgt gcaactgcgt tgagtgtacc ggcagattgt ttttcaccta
     6721 aagaatttga ttcaaaaaaa gagtatgaag aagcaatttt gcataaattg aaagaaaaaa
     6781 agatcgattt gatcgtatta gctggatata tgcgaatcat tggaccggta ttgctgaaaa
     6841 attatgataa aagaatcatc aatatccatc cgtctctttt acctgccttc cctggtttgc
     6901 atggcatacg tgatgccttt gaagctggag taaaagaaac gggtgtcaca attcattata
     6961 tcgaccaagg tgtagatact ggaccaatca tccggcagga aaaagtgaga attgaacagg
     7021 aggacacgtt cgattcttta gaagaaaaaa tccatagagt agagcatcga atctatccag
     7081 aagtgattag tgaaattatt gaaaatggag gctatctgaa atgacaagag cattgatcag
     7141 tgtatcagat aaaactggga ttgtagcatt cgcccaaggt ttgattgaac aaggagtaga
     7201 aatcatttca acaggaggaa caaagcatgc gttggataaa gctgggatca aaacgctttc
     7261 gatcgaagaa gtgacagggt ttcctgaaat gatggatggc cgtgtcaaaa cacttcatcc
     7321 taaaatccat ggtggtcttt taggaagacg cgatctccct agccatatgg aggcaatgga
     7381 aacggaaaat atccaaccaa tcgactttgt ttgtgtcaat ttatatcctt tcaaagaaac
     7441 gattatgaaa ccagatgtcg aaatagcaga agctattgaa aacattgata tcggtggtcc
     7501 tagtatgctc cgtagtgcag caaaaaacca tgaatttgtc acctctgttg ttgatccatc
     7561 tgactatgaa gaagtacttt cagaattacg tcagtatggg catacttcac tttctttcag
     7621 acaaaaacta gcggcaaaag tctttcgtca tactgcggca tatgatgcac tgatcgctca
     7681 atacttaacg gattgggtgg aagaaaaaga accggaaaaa ttgacactta cgtatgaacg
     7741 aaaacaaacg cttcgttacg gtgaaaatag ccatcaagcc gctgcctttt atcagtccgc
     7801 tcttctggaa aaatattcga tcgcagcagc aaaacaactc catgggaaag aactttctta
     7861 taataatatc cgagatgcag atgctgcgct acggatcatg aaggaatatg acgagccaac
     7921 ggtagtcgcc ttgaaacaca tgaatccttg tggtattggg acagcatcta cgattttaga
     7981 agcgtgggaa gcagcgtatg aagcagatcc tgtttcaatc tttggtggga tcatcgtttt
     8041 gaatcgagaa gtagatctgc caacagcaga acagatgcat aagctgtttc tagaaatcat
     8101 cattgcccct tcttatacac aagaagcttt ggaattactg caaaaaaaga aaaacatccg
     8161 attactgaca gtagactttg cgcaggaaga aaatcaaaat cgagaagaaa cggtctcagt
     8221 cttaggtgga cttcttgtac aagatcaaga tctggcaata gaaaatgaag aagagtggaa
     8281 agtcgtgaca aaacgtaagc cgactccagc agaagcaaaa gcattagcgt ttgcttggag
     8341 agctgtcaaa catgtgaaat ctaatgcgat cgtactggca aacgaacgtc agacagtagg
     8401 aatcggagcc gggcagatga atcgagtcgg ttcagtaaaa attgcgattg aacaagcgca
     8461 agagaaaatg gaaggtgcag ttttagctag cgatgcttat tttccaatgg atgattcggt
     8521 tgaatatgca gccaaacatg gtataaaagc aatcattcag ccaggaggaa gcatcaaaga
     8581 ccaagattct attgatatgg cgaataaata cgatctagca atggtcttta ctgatgttcg
     8641 gcattttaga cattagagag gagtcggata aatgaacatt ttggtgatcg gtgcaggtgg
     8701 tcgtgaacat gcaataagta aaaaactttt agccagtccg aaggtggata cggtgtattg
     8761 tgcgccagga aatccgggga tgacaaaaga tggtatcaat tgtgtcgcta tcagtgagcg
     8821 agatcattca gcgttaatcc attttgttaa gaaagaggct attgattgga gtatcgttgg
     8881 tcctgaagtt cctttgttaa atgggatcgt cgatgatttt atggcggaag gattgaaaat
     8941 cttcggtccg acaaaagcag cagctctgat cgaaggttcg aaaagttttg ctaaacaatt
     9001 aatggatact tatcaaatcc cgacagctaa atccagaagt tttgaatgtt acgaaaaagc
     9061 agcagcgtat atcaaagaaa atggtgcgcc aatcgtagtg aaagcggacg gcttagcagc
     9121 tgggaaaggt gtagtcgtgg ctaccacagt gaaggaagcg ctggatgcgg cagaacaaat
     9181 gttgaaccag catcgatttg gggatagtag caaaaaaatc gtcgtagaag agtttttggc
     9241 aggagaagaa ttctcccttt tggcgtttgt ccgtaatacc aaggtgtatc cgatggtcat
     9301 ttcgcaagat cataagcggg catttgataa cgataaaggg ccaaatacag gcggaatggg
     9361 tgcttattca ccagtaccgc aaatttcaaa aaagatgatt gaaaaagctg tggaagaagt
     9421 tttaaaacca gcggctttgg gaatgaaaga actaggacgc tctttcacgg gtgttttata
     9481 tgcgggtttg attgctacga aggaaggacc aaaagtgatc gaattcaatg cgcgtttcgg
     9541 tgatcctgaa acgcaagtcg ttctttctcg gttgaagagt gactttgcgc aagtaatcga
     9601 tgatttatta gagaatcgta cagtagagtt aaagtggcag caagaaggat acaatgtagg
     9661 cgttgtggtt gcagctgccg gttaccccga agaatatgag acaggaatgg ttctaccaga
     9721 ttttctggaa gaagagcttt ctgtttacta tgctggagtt tcctcaaata acggcaaact
     9781 agttggagca ggaggacgtc tttatttagt agaagcatac ggtgagacga tcaaagaagc
     9841 acagaaaaaa atttacaccg cgttaatgga taaaacgaca gatggaacat tttatcgtac
     9901 agatattgga gaaaaagctg ccaatagcga actgatcgtg aagtaaatca gctgcttcca
     9961 tttataaaaa atacaacaaa cataatcgga agcgccgatt ttagtgaagc cttttcataa
    10021 agagtgtggt acacttttgt tgtgtatata aataaggagg agttcagatg aaaaaaattc
    10081 aattaggtac ttctgatcag aaagcagctg cagtgatctt aggctgtatg cgtatcaaca
    10141 atgcggaaga tccagtaaaa gtgattgaga cagcttatga aaacggcatt gatttttttg
    10201 accatgcaga tatttatgga aacggtgaat gtgaagaaat ctttgcagat gctctagcaa
    10261 aaacatcaat caaacgagaa gatcttttta ttcaaacaaa atgtggaatc gttccaggtg
    10321 tgatgtttga tttttctaaa aaacatatca ttgaatctgt agaaaaaagt ttgacccgtc
    10381 tgaaaatgga ttatgtggat gctttacttc ttcatcgtcc agatacattg gtagaaccag
    10441 aggaagtagc tgaagcgttt gatgaattgg aacggtcagg aaaagttcgt tattttggcg
    10501 tcagcaatca aaaaccaatg caaatcgaat tgctgaaaaa atatgttcgt caaccattag
    10561 tagctaatca gctgcaattt ggcttgaaac atactggaat gatcgatcaa gggattcatg
    10621 tcaatatgac agatgaagca agtatcgatc atgacggtag tgtacttgat tattcccgat
    10681 tgcatgatat gacgatccaa gcatggtcac cataccagta tggatttttt gaaggtgtat
    10741 ttatcgggaa cgaaaaattc cctgaattaa accaaacatt agaagaacta gcagctaaat
    10801 atcaaacgac accaactggc ttggcttctg cttggatttt acgccatcca gccaatatgc
    10861 aagtgatcgc cggtacgatg aacgtagggc gaatcaagga aatcgctcaa gcttcagaaa
    10921 ttaatatgtc tcgggaagat tggtacgaat tgtaccgtgc tgctggaaac attttacctt
    10981 aaagattgtc ataagcaggt caaaagggct ataaattaaa aagacagaat aacttgaaag
    11041 ctgttttcag cttcgagata ttctgtcttt ttttattgca aatggaaaga gtatcattgt
    11101 acggatttcg tttattagtt atttttgttt ttaaggaaaa gctgtgtccg atattctttt
    11161 ggagaaatcc cttgtttttt cttaaataaa taactgaaat agttagggtc attataacca
    11221 atttcaaaag caatatctga aatacgatag tctgattgct gcaataattt ttttgctaat
    11281 tcgattcttt gttctgtcag ataatcgatg aatgtttggt taaacgattg agaaaaaatc
    11341 gtactgaaat gagagggact taatgcaact tgttgagcga cccaagttaa ggacatatct
    11401 ggatcggtaa aattttcttt gataaaatcc aatgcatggt tcaaaattgg ctgatatttg
    11461 ttccttgaag gctgtgaggg cagagcagtt aagaaagaaa tgaacatata aactgtcgaa
    11521 cggtaaagcg agtcgtcggc gctgattgct aagcgttgac tggtggatgt gcaatgaacc
    11581 atttcttgcg ggattttctt ttctttcagg cagacatgct ggagtagctt agtcaagaca
    11641 aataaacgat ataatcgtgt tcgagttggt gtgccttgtg gtttcgtcaa gtgttcgatg
    11701 tacttagata cttcgttttt cgataatgaa gacaaatcaa gtgagaggtc aaagtaaaag
    11761 acagaatcaa ctggaagttt cgcttcttga atcaaatcct catagctgat aatttgttct
    11821 ttaagggaac tggcggtttc aagtatcatc tgcgtgatct caaaagatgc aggtaattcg
    11881 ctgattcttt ctgcgacagg accaatggcg acagaaaaat tatttttttg tcccttttct
    11941 aattcatgga ccaacgtttg ggctacctga taactggtct cgatcacatt ttcttcaaga
    12001 gaatcaacta ttaaaaattt gatgaatcga gaggaaacag cagagaagat cacttgttga
    12061 ttctccctaa ataaaaactt caaataatca ctgagatgag cgtaatcttc aaagtctgta
    12121 ctgacttggt ttgctgctaa taatacggtc atttttttcc ctgctaaaga gcgattgaaa
    12181 gattcagcaa gtttcaatgt ttcactgatc gtaagtgagc ctttgaacaa accgttcaag
    12241 aaatgatttt ttttcaattc gaaaacaaga tcagaagaag tgtgcgtttc tttttgctgc
    12301 aaatctaaag cagttactgc tttttctatt gcttgtttca attcgctact tttaatcggt
    12361 ttaagcagat aatcatctgc ctgtacctgg attgctcctt tcatataatc gaaatcgtcg
    12421 aatccactaa taaatatgag acgagtccaa ggaagtagtt tccttgcttc ttttgaaaaa
    12481 gtcaacccgt ccatgaaagg catttggata tcagtcagaa tgatgtcggg ctgtaagtcg
    12541 ataattgaag acagtgccat ctcaccatct ccagcttcgc cgacataagt gattggataa
    12601 taagaaccaa ggacagttat ttgttcccgc aagttattgc gaatgaggta ttcgtcttcg
    12661 atgataaaaa ctttatacat gtttgtttcc tccaattgtt ttgggagata gagggatcat
    12721 cagtgtgaca gttgttcctt ctcggtattt gctgtcaatc gtgataggtt tttgatcatt
    12781 tccgtaataa agaaggagac gcttgtttac gttgtaaagt ccgtagccga tactgaaatc
    12841 tgaatcgatc cctttggcta actctttacg tatttctgcc agtttttctg cagggatgcc
    12901 aatcccgttg tctataactt tgaattggat catctcatca attttgcatg tggtaatacg
    12961 aataaatcct ttgtgacgga cgtacttcgt tccatggtat acagcatttt caatcaatgg
    13021 ctgcaagatc atttttaaaa tagaaacagg aaaaagtgtt tctggtatat cgatcttata
    13081 gtcaagcata tggccgtatc ttatttttaa gatgactaga taatcattga tatggctaat
    13141 ttctttttca agaggaatcc agtcttgtcc tttgcttaaa gacgtacgaa aaaagccgga
    13201 aagtgcatgg gtcatagtca gagcttcttc aaagcagtct tgttcgatca aagaaataat
    13261 ggcatccaaa ctgttataaa taaaatgggg agtaatctga gcttgcaata cacgaacttc
    13321 actttgtgct aaatggtatt gtttcaaagc gttctcccgt aaagactggt ttagatcttc
    13381 cgccatttta ttcatgctag cggtaagtgt ttgtaattcc agtgtttccg gcagggttaa
    13441 acgaaagcca aggttccctg aagaaagttg ttctgacatc tcgatcagct gattgatcgg
    13501 ttcttggacc tttctctcta aaaagcgacg attctttttc gcatagaaaa aaatggtact
    13561 aagaactcca agttccacta ttgttaatag aatgagcgag cggacaagtg tcgtattttt
    13621 tttgcttgct aagtcgatct ccacacgaac aaaatcttgt aagatatctg acagcagttg
    13681 agtgacagaa tctacctgat tgatgatctc ttcattggta tccataaaat cttttgccaa
    13741 gatattattg atcagctcgt tttgataatc ttccagtgta tcgatcgtac gtagcgatac
    13801 atctaacgta ctaatttctt taagtgtatt tgtattattt ttgatctctc gaatatcatt
    13861 cctaagttct tctaggatat tttcctcatt gaactcatct acggttactt ggccagaaat
    13921 caagtcccac atactttcca aaactttttg atcaaccttc aaagcaattg catctgcttt
    13981 ttggatattt gcgatcgtct gttgataaat caaaagctgt tgcgaatata acagtatgct
    14041 cagtagcaaa gggataacag caaatagaag catggctttg tttgcttggg caaaaatctg
    14101 ttttatgctt aaattttttg tcatgatact tctccagtca ataatttctg gttgggatgt
    14161 tagctatatt ctgctgggaa aagacagtct cggaaataaa aatatcttta ggtaatgttt
    14221 ttcctgtttg aatatttcca aaataacgag taacggtatt ggctacatac cagccgacat
    14281 caggtttaca ctcgactaca caattgacga ttcctgagcg tagcttctcg atcatatctt
    14341 cttgtccatc aatagttaca atgatcaaat cttctccagg tttgattgat gtttctttta
    14401 tcgcagctaa agcaccgtga gtcatttcat cactatggct gtataataca tcgatttcgg
    14461 ataaatcatt tgtttctaaa tatcgtttca tctggtcttt tcctttcatc cgaataaaat
    14521 caccgacaat tcggtgaaca atagtcatcg gaggtttatg gtcgatggtt ccatcttttt
    14581 ttagggttgg tttacgattg attgattcca aaaatccttc ggagcgtagc ttggtcggtg
    14641 tggagttctc tgaaccagca agttctagga ttctgatttc ctgtttcttt gaatttgtga
    14701 agtgattgct gacaaataaa cccgcccgat ttccttctgc tttgaaactg gggccaatgt
    14761 gggtgacgaa aagctcggga tcattcgttt gaatatgtct gtcgacaagt atgacaggga
    14821 tccctgctcg ctttgcttct tttaatacag agtcccaccc ggattcttgc aaaggagtaa
    14881 aaatgatcat atccacttta taggcaatga acgtccgtat atcttggatt tgccgttcct
    14941 gattcatgta gccatttcgg tagagaactt ggtaatcttc tttttcaagc gcagtgataa
    15001 ttgattcagt atgctttttc cgccagctac tctccgttcc tgattgcgaa aaaccaacga
    15061 ctagtgggtc aggtgcttcc tttgtattag gtgtacaccc catcatttgg taagcgatta
    15121 caacaagggc taaaaaaaat aaaacacgtt tcactttaaa tcactccatc tggatttgtt
    15181 agtactatct tatcagataa aagggaaaat caaagtcaaa aggaatcaat cgaaaaaaat
    15241 ccaagaaact taaaaaaatc caagacaaaa aaagtacacc taaaaaattt aaagtaaatc
    15301 ccataaaaaa atcaatgtta acgctttctt tattagctat actcggtgta tcaattgaaa
    15361 tgggggataa aaaatgaaaa acaaatttag aaagattgtt gggactatcg ctattttgac
    15421 cgcaagcatt ctattaagtg cgtgtggcaa ttcaggatct gcagatgata gtacggggta
    15481 tgtaggaatc gctatgccga ccaaatcagc agaaagatgg atcgctgatg ggaacaacat
    15541 ggtctcggaa ttagaaaaac tgggttataa aacagatctt caatatggag aagataaagt
    15601 agaaaatcaa gtagcacaaa tagaaaatat gattacaaaa ggggtagata cacttgtgat
    15661 tgcttctatc gatggttcgg ctttaactga tgtgttagct aaagcaaaag aagcagatat
    15721 caaggtgatc gcctatgatc gtttactgat gaattcagaa aatgtcgatt attacgcgac
    15781 ttttgataat tttggagtag gtgtatcgca agcagcttat attgaagagc acttaggatt
    15841 gaaagaagga aaaggaccat tcacgattga attatttggt ggctcgcctg acgacaacaa
    15901 cgcactaatc aattacaacg gagtgatgtc tgttttacaa ccatatatgg acaacggtca
    15961 gttggtcgtg ccatctggac agacgagttt cagtcaaatc gctacgcttc gctgggatgg
    16021 ttctacggcg caagcaagga tggataatct gttaagcgca aattatactg accaaacatt
    16081 ggatgctgtt ttgtcgccat atgatccaat aagtttagga atcatttcgt cattaaaagg
    16141 cgtggggtat gggagtgaaa gtaaaccttt acctgtgatt actggacaag atgcgacagt
    16201 tgctggcgtt aaatcgatca ttgccgggga acaaacacaa acgatcttta aagacacacg
    16261 tatcttagca aaaaatacga ttgaaatgat caaagcaatc agtgatggag aagaggttcc
    16321 ggtcaatgat actgaaactt atgataatgg ggtgaaaact gtccctacgt atttagcaaa
    16381 tacagtatct gtcgataaag ataattatca agcagagctg atcgacaccg attattataa
    16441 agaatccgat ctgaaaaatt aagaggaacc aaacaaggaa ctaaagagat ctaagtcaga
    16501 gagaggataa gaattatggc ggattgtatt ttaaaaatgg cagatatcac caaaaaattt
    16561 tctggaatcc gagcgttaga ttgcgtgaat ctgatgattg aacgtgggga aatccatgca
    16621 ttgtgcggag aaaacggagc gggaaaatca acattgatga atgtcttgag cggcttgtat
    16681 ccatatggct cctatgacgg ggaaatcttt tatgatggaa agctgtgtca atttaaagat
    16741 ttgaaagata gtgaagaaaa aggcatcgtc atcattcatc aagaattagc actaagtcct
    16801 tatctttcaa taaaagagaa tatttttctc ggaaatgaac aagcagaaag aggcgtgatt
    16861 gattgggaac tgacagaaaa gaaaacatat gaactgctga ataaagtcgg tttgtctatc
    16921 gacccgaatc ttctagtatc ccagattggc gtagggcaac agcaactagt tgaaatagct
    16981 aaagcattat caaaatccgt ccgtctgttg attttggatg agcctaccgc tgcattgaat
    17041 gaagaagaaa gcgcgaattt gctgagattg atccgagagt tgaaagagca aggtgtaacc
    17101 tccatcatta tttcacataa gctgaatgaa atcgtggata tcgcagatcg aatcaccatt
    17161 ttacgggatg gacagacgat tgagacacta gaaaatgaaa tgatcagcga agagcatatt
    17221 attcgcggaa tggtgggcag agatttagtt gatcgttatc ctgaaagaaa accggatatt
    17281 ggagaagttt attttaaagt agaagactgg acggtctatc accctcttga tcacgagcga
    17341 atcataaacg accacatcaa cttgcatttg aaaaaaggag agattgtagg aatagctgga
    17401 ctgatgggag cgggcagaac tgaattcgct atgagtctat ttggacattc ttatggaagc
    17461 aagatatccg gacgtatatt aaaggaaggg gcagagatat cagttaagac cgttccttca
    17521 gccattgcaa acggactcgc ttatgtctcg gaggaccgaa aatctttagg actgaatctg
    17581 ctgatggata ttcgggaaaa cgtgacgctg agcagtttgg ataaaataag tcaaaaagga
    17641 gtattagata aagaaaaaga agtaactgag gcagaaaatt tccggaaaaa gatgcgtata
    17701 aaaacaaact ccatccatca aattgtcagt agtcttagtg gtgggaatca acaaaaagtc
    17761 gttttatcca agtggctgat gacacaaccc gatattcttt ttttggatga gccaacccgt
    17821 gggatagatg ttggggcaaa atatgagatc tacacgatta tcgaagaaat ggcagcaaat
    17881 ggcaaagctg tctgtcttat ctcttctgaa cttcctgaaa tattagggat gtgtgatcga
    17941 atctatacga tgaacgaagg acgtattaca ggagaagtct cgcgagaaga tgcagatcag
    18001 gaagtcttga tgaaattaat gacgaaagaa gaggtgggtt aagttggaac aggcaaaaaa
    18061 tcttacggat aaaaaagaga aaaaactaaa tttgaaaagt agagcagctg atctttttgg
    18121 tcgctacagc atggtgatca ttttagcggg actacttatt gcgtttcaat tgatgacgga
    18181 tggtattttc tggcgtccat tgaatataac taaccttgtt ctgcaaaata gccatatatt
    18241 agtactagct gcagggatgt tattagttgt gctcttaggt catgtggatt tgtcggtagg
    18301 gtcagtgatg gcgtttgttg gtgcaattgc gggcatgatg atggtcaatc gccaaatgaa
    18361 cccgtggatg acagttatcg catgtcttgc agtaggcggg ctgatcggcg catggcaagg
    18421 tttttgggta gcttatgcag ggataccggc gtttatcgtg actttagcag gattgctgat
    18481 gtttcgtgga ctgacacaag ttgttctagg aggccaatct ctagcacctt ttccagctgt
    18541 tttccaaaaa atgtctacgg gttatttacc agacttaagc gaaggaagca gccttcactt
    18601 cttgacattg attttaggaa tgatcatcac gatcgtgtta gttgttggtc aatggcgaac
    18661 aagaaatcgt cggaaaaatc atttatttga agtggaatct ccaacagctt tcttggtaaa
    18721 ggcggtattt acagcagtcg ttgtactagg aataacttat atctttgcct cttatcaagg
    18781 gtatccgatt attttgatca ttcttggttt gatcgttgca gcatatgggt ttttgacaaa
    18841 taaaacagta gcaggtcgac agatttatgc gacgggcgga aatcgaaaag cggctgaact
    18901 atcagggatc aaaacaaagc gaatcacgtt ctgggtattc gtcaacatgg gagtcatggc
    18961 agctttagct ggattagttt tagctgttcg attgaatgcg gcaacacctc aagcaggtac
    19021 atcgatggaa ctggatgcaa tggcggcagt atattttggt ggtgcttcga cttccggcgg
    19081 cattggaaca atcatgggtg cgattgtcgg tggcttggtc atgggtgtat tgaacaatgg
    19141 catgtcgatt ttaggcgtcg gggtagactg gcagcaggca atcaaaggat tgatcttgct
    19201 tttagccgtt gtgctagact tgtataataa gaaaaaacga acaacgtagg agaacgccgg
    19261 ttattcagaa aaaagactgt gacaagacat ttgtcacagt ctttttttcg ctaaaagcga
    19321 acattcattg tttcgtttgt atagactaaa aataatatat gtttttttac cattcgtata
    19381 aattctcttt ttgctcttga tttttttgct taattgagtt aaattacata gggcgctaaa
    19441 aaaagccttg attaaattaa taatgttcgt gttttgctgg atgtgaacat caggaggata
    19501 atgtaatgaa agtatttgat tacgaagata ttcaattaat tcctaataaa tgtatcgtaa
    19561 atagccgttc tgaatgtgat acaacggtca cattaggtaa acatacattc aaaatgccag
    19621 tagtgcctgc caacatgcag acgattattg atgaaacaat cgcagaattc ttagcggaaa
    19681 atggttattt ctatatcatg catcggtttg atgaagcagc tcgaattccg tttatcaaaa
    19741 aaatgaaaaa gcgtggattg attacctcga tcagtgttgg cgtcaagaaa gaggaatatt
    19801 cctttataga gaagttggca gaagaatcgc taaatcctga ttatatcact atcgacatcg
    19861 cgcatggaca tgctaactca gttattgata tgattcagca tattaaaaaa tatttgcctg
    19921 aaacttttgt gattgccggt aatgttggta caccggaagc cgtccgtgaa ttggaaaatg
    19981 cgggcgcaga tgcaacaaaa gtcgggattg gtccaggaaa agtatgtatt acaaaaatca
    20041 agacaggctt tgggactgga ggctggcaac tggcagctct tagatggtgt gcgaaagcag
    20101 ctcgcaagcc gatcatcgct gacggtggga tcagaacaca tggcgatatt gcaaaatctg
    20161 tccgttttgg tgcgacaatg gtcatgattg gttcattgtt tgccggtcac gaggaatctc
    20221 ctggcgaaac aaaagtcgaa aatggaatcg tgtataaaga atactttggt tctgcttcag
    20281 aattccaaaa aggcgaaaaa cgaaatgttg aaggaaagaa aatctggatc caacacaaag
    20341 gatctttgaa agatacacta gtggaaatgc aacaagacct tcaatcatct atttcttatg
    20401 ctggaggaag agatctggaa gccattcgaa aagttgacta tgtcatcgtg aaaaattcga
    20461 tttttaatgg agacacaatc taaaaaataa ataaaatatt aggatgttgt gaaaaagttg
    20521 tcctgcatta agtaataaga acagccaaac aaaaatagtt ctttatatgt ttt
//
LOCUS       DAXWLF010000035        19833 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXWLF010000035 DAXWLF010000000
VERSION     DAXWLF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.035"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(29..226)
                     /locus_tag="ACGE4U_000988"
     CDS             complement(29..226)
                     /locus_tag="ACGE4U_000988"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFF8393170.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(372..1250)
                     /locus_tag="ACGE4U_000989"
     CDS             complement(372..1250)
                     /locus_tag="ACGE4U_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8393171.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1289..2407)
                     /locus_tag="ACGE4U_000990"
     CDS             complement(1289..2407)
                     /locus_tag="ACGE4U_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFF8393172.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2404..4503)
                     /locus_tag="ACGE4U_000991"
     CDS             complement(2404..4503)
                     /locus_tag="ACGE4U_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFF8393173.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4554..5375)
                     /locus_tag="ACGE4U_000992"
     CDS             complement(4554..5375)
                     /locus_tag="ACGE4U_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8393174.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5372..6181)
                     /locus_tag="ACGE4U_000993"
     CDS             complement(5372..6181)
                     /locus_tag="ACGE4U_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8393175.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6202..6696)
                     /locus_tag="ACGE4U_000994"
     CDS             complement(6202..6696)
                     /locus_tag="ACGE4U_000994"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8393176.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6708..7208)
                     /locus_tag="ACGE4U_000995"
     CDS             complement(6708..7208)
                     /locus_tag="ACGE4U_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8393177.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7273..9969)
                     /locus_tag="ACGE4U_000996"
     CDS             complement(7273..9969)
                     /locus_tag="ACGE4U_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8393178.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10397..11470)
                     /locus_tag="ACGE4U_000997"
     CDS             complement(10397..11470)
                     /locus_tag="ACGE4U_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8393179.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11467..12294)
                     /locus_tag="ACGE4U_000998"
     CDS             complement(11467..12294)
                     /locus_tag="ACGE4U_000998"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8393180.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12295..13101)
                     /locus_tag="ACGE4U_000999"
     CDS             complement(12295..13101)
                     /locus_tag="ACGE4U_000999"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8393181.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13113..14198)
                     /locus_tag="ACGE4U_001000"
     CDS             complement(13113..14198)
                     /locus_tag="ACGE4U_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393182.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14215..14757)
                     /locus_tag="ACGE4U_001001"
     CDS             complement(14215..14757)
                     /locus_tag="ACGE4U_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFF8393183.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15015..16004
                     /locus_tag="ACGE4U_001002"
     CDS             15015..16004
                     /locus_tag="ACGE4U_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFF8393184.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            16001..16738
                     /locus_tag="ACGE4U_001003"
     CDS             16001..16738
                     /locus_tag="ACGE4U_001003"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFF8393185.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16735..17286
                     /gene="coaC"
                     /locus_tag="ACGE4U_001004"
     CDS             16735..17286
                     /gene="coaC"
                     /locus_tag="ACGE4U_001004"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFF8393186.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17302..17934
                     /locus_tag="ACGE4U_001005"
     CDS             17302..17934
                     /locus_tag="ACGE4U_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8393187.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18019..18666
                     /locus_tag="ACGE4U_001006"
     CDS             18019..18666
                     /locus_tag="ACGE4U_001006"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFF8393188.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18671..19654
                     /locus_tag="ACGE4U_001007"
     CDS             18671..19654
                     /locus_tag="ACGE4U_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFF8393189.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         6078 a         4253 c         3460 g         6042 t
ORIGIN      
        1 atttatttta tacggctgaa aaaatctatc accaaaaaaa ttcaaacatt aagccaagga
       61 ttatgaagtc aacatttcga aaaatgaaca tacattggta agcaactata actattacca
      121 cagacataaa gtaacacaat atattactat ttaagtgtac gccaccagtg aatatcctca
      181 aggtatacag cgcacaacaa aaagaaaaaa ggaaacgcag aagcatttcc tttcagtgat
      241 ttaatgaaaa caacacgatt tgacttagag cctttttatt tgaattcatt ttcgctttat
      301 ccaacttcat ttgatcattt gattactcgg ttaaatactt ttgacatgag tatcaatgaa
      361 acattcatta attatggttg cttatttctt accgcttttc tggccaatgc taatccgccg
      421 attattccgg cattatcacc taattcgggc gtaataatgt agctatcaat tggtggtatt
      481 tcaacatatc catttataag ctcaacaaat ttatcctgaa cttttttctt catgtgttct
      541 tgtttcatta caccaccgcc aaaaataatt ctatctggcg aaaacatcag tgttgtattg
      601 tatgcacatt gggcaaggta aaaggcttca atctgccaaa acgaatcgtc agccaataaa
      661 ttctggcctt ttacacccaa ccgtttttca atcgctggtc ctgctgccat cccctctaaa
      721 caatcttggt ggaagggaca gtttcctgaa aaattgtcct ttggatgtcg cttgatcaac
      781 atatgcccca tttccggatg actgaaccct tccacgaaag tcccattgaa caaggcacca
      841 cctccgatac ctgttcccac agtgaagtat gcaacacctg ctaacccttt gccagaacca
      901 gcaacatatt caccatagca agccgcattg acatctgttg tccattcaat cggaatattg
      961 aaatattgct ttagcgttcc tacaaaattg aaattttgcc aagcaagttt tggtgttgat
     1021 gtgatatgac cgaaactcgt tgatttgcat tgaatatcaa ttggaccaaa gcagccgacc
     1081 ccgatgcctg ccaattgttc cttaaaaggt ttaaaaaaat caatgacctg cttcattgtt
     1141 tcaactggag ttgttgtggg aaaactgact ctttccaaaa tcgttaattc ctcagtaccg
     1201 actgcacaga caaatttagt ccctccagct tcaattgccc catatatcat cataggttcc
     1261 tcccttcgaa tatgtgaaaa acacttcatt aacgcggcaa gcgttctcga tcagtgaaat
     1321 gacgtttagt atttcctgaa gcttctgttg aataccagta agcgacacta gcaacatcgt
     1381 cttgccgctc aaataagcga acatcatcgt tgccgatttg ttgtaaagtg accgataaat
     1441 cttcggtaaa acagatgggg tcttgaagat gccaacgata aagaccatgt cgtggcattg
     1501 cgtcgtcacc aaaggcatga acggcattca attttcccgt ttcaaaacga tctcttgtgg
     1561 catctctatt tgattggtaa ggatacccta aaaaggctgt tgaatagttt ttcgcatgtg
     1621 gacgtccgaa ctcatcgtac tcatgaaagg cccacgcccc gccaaaataa tcttctgaac
     1681 ctgttgaggt tacggtcgga tactcactat ccccatcaat gtagaattta aactcgcctt
     1741 cgccccacca ataacgttct aggcaggtaa gcgcaaggta agtacccacg taatagcctt
     1801 gaccctttaa tcgatcaatt aacttataat cctcctgtaa tttggtaatg cgttcccgat
     1861 gccaatttgc gtgaaaatat aggctatttt cttccaattc gtccaccaaa gcgtaattta
     1921 tcgtataaaa aaatgaggac atatccttcg gatgtaaatt ctcgatcgta attttagcgg
     1981 atgtttggaa aggcatttcg aaataggaat taaagccccc tgtaggatta accactatcg
     2041 gcaacgagtt cacctcgcaa cgctctccaa acccattaca aaagaaatcc cctaaaggac
     2101 tttcaacagc agggctatcc gacccatccc aataaattct taagattaaa tcacgtaaaa
     2161 caaaactgcc aaattctgtt cgatcacgaa tcgtcatcca catatgacga attattcccg
     2221 ttccagtaat atccgcaatc acagtttctt caccagaagt cagcggaatt gcgccacgac
     2281 ctttgcggct tggtcctagc tcgcttggca acatcgcagc tttacctttc tcgcctgttg
     2341 gattttcggg agtgattgct cgactctgga tttttttaaa ttgggtaata ttttttagta
     2401 tactcatctt tttctccttt gaatgtgaat cgtttttccc ttttctacgt gaagaatttt
     2461 tttcgtatca cttattttca atggcttttc tgacaaattt gaaatactca actcggattg
     2521 cgtacattca atcgaaatga aggcttgacc gaacttcaaa ttcttccaag aaaacgttaa
     2581 attatcaaca ggatcaattt gcaatactcc ttttttgaat ttcaatccga aatattgact
     2641 gatcattagg ctgacaaaaa gacccgatgc ccagccgcat ttgccgcagc caaagtctcg
     2701 cacgacttct ccttgttgag cctctaagcg atacggccac caccaccacg aaccgtctaa
     2761 atctgccaaa caaattagtt cttctaatcg ggttacgaaa gcttgagggt tgtgcagctc
     2821 gctcattaaa acagtgataa agccaggaaa agtggcgccg gattctaatc cccaagtgat
     2881 gcctttgatt tcctcgctat aggttggatt gaaagaacta gcagaaaagg ccatggtgtt
     2941 ctgatataaa tgatctttat tatctaaaaa ttgataaaaa ggcatcaacg tagtgtctga
     3001 ttcttctcca tcatgcatca ataaattaat ttttccatcc acaatcacat cacttaaaaa
     3061 gattaaccct tgctcaagaa tcggtttttg atagtgcttc actgaatagg ttgttttctc
     3121 ctcatcaccg attccctcca aaaattgctc accaaaagga ccatcgatag tcatttgttt
     3181 acaaattgat tcctttagtt tacaggcaat gtttttgtat cgcttgctaa gactcagttg
     3241 ttctagggct tcaaaaattt ctgcaagtcc aatacaggcc ttccaaagac aaagattgct
     3301 gccggtatga tactttccaa gtgcaaaggc gtctgataac caaacagatg gaaaaagcat
     3361 tggttcccct gaccgacgtt gagctaatat gtcctcaata attcttgaag cattcagtag
     3421 aacctgcgga caccgctcaa agaattttgt atccccagta tgttcaaaat aaagactagc
     3481 cagtaacgca ctggataacg aattactcag agaatgattg atacctcctg taaattttga
     3541 accaggaaac ttaacgccat attgatcaaa ccatagaacc gttttttgac ataactccgg
     3601 ttcaaacatg gtgaatggca atgatgcgta atacatatcc ttattccaaa ttcgattagt
     3661 agcaggatag ctgccccaat tgctgccgac gacctgtccc tgtcgattca taccgaaact
     3721 agaaaagctt tgataaagtg cacgacgata gagatggaca actctatcgt ttggcattga
     3781 cagctgccca aatagctgag caaaatagtt caatgtttcc ttcagccatt tttcgatatc
     3841 agattcctga aaagtcatta cttcttcaaa agcatttggg tccacaaaag caatggcgaa
     3901 gcttgccact tcctgttgtg cgatcgtgtc cttatttcct ttttgagaaa tcaacacatt
     3961 ttgctgatcc gaatacttct cttgatacga aggcacatct ttcactgaaa cttgaaggct
     4021 ttcgtgtgtt tcattcacaa tgtaaacctg ttgaacaagc atacttaaac gcttattagc
     4081 cagtatggga gcaaaggata ctacaatgac cttaaacgtc gaataatgat gaatatatct
     4141 aggcaaacag tcttgaatca catcgacttc aatacgatga tccgattctg acaaatggta
     4201 gacaatcccg tttacttcaa cctttaagga taacgaattg gtttgagaga gctgtttatt
     4261 gacccaaaca cctggttttt cctcagttag taaattttct cgataaaagg tcaacgtttg
     4321 aatcgtcccg caaacatcaa ggtttgctaa tgtcttttga ttacttacat cgaaggtcaa
     4381 tccctttaca tcaggaatat tccgatagta gctgtattcg tttgaagtag tcacatttcc
     4441 gtcgtttaac tcagcattaa aaaatgggtg tttgggtgaa taaattactt cattaatagt
     4501 catcttttcc ctccttgcta aaaaaactgc agcctagtag ctgcagcttc ctactatgcg
     4561 attccaagta atgacaataa aattcccaag acgaccattc caaaaataat tttgatgacg
     4621 ccgacttttt tatctagtaa tttgaaacag ctcaatgtta ccaataacgg tacaattcct
     4681 ttaaaaatac tatcgaggat ttcttggaca ttcattagtt cttcgccctt cattgaaaag
     4741 ctcaaaacgg tattgaattt gaccatactg atcgtcattg cacctaccat gatcagaccc
     4801 aagatactag cagcttttgt cagaatttca attaacccgt tcgcatacag cttttcaata
     4861 tatttagagc ctaatgaata gcctaaaacg ccgccatagt atcgcatcaa gtaattaggt
     4921 atattgaaca taactaaaaa gataattggt gccaaaatat ttccggcatt ggctaagttg
     4981 atcgctagtc ccgcggtaac gacacgccaa actccccaga acaatgaatc gccaattcca
     5041 gccaatggtc ccatcaaact cgttttgatc gcgttgatac tttccacgtc aaaaccagct
     5101 ttttcagaat tttccttctc catcgatgcc gcaatcccca tgacaaagga cgcgcaagga
     5161 atggtcgtcg aaaaatactg tgtatgacgt tccagcgccg cagctcgatc ttcttcactt
     5221 ttgtagaaat gattaatgaa gggcatcata gaataggcga aaccaccgcc gcccatcgtc
     5281 accggattca ccgaagcata catagtcatc gagcgccaaa acatcttccg aaccaatttt
     5341 ttctcatctt tagaaattcc atcagctaat ttcattgaaa gaaatcctcc tcttcttggt
     5401 caagcgttgc atttacagca gcatcgcttt gaactgcatg aacagatata ttttttagtt
     5461 gtagatctct ttgggctata acaattgcaa taatcgttcc aactgcggca attgcaatca
     5521 atggtaactc gagatacgcc ataacaacgg ccccaaagaa aaaccacaca gcaatatttt
     5581 tactccaaag catccgtaac agcatcgcca tcccaactgc cggcagcaga tttccagcga
     5641 ctgttaatcc gttcatgatg acctctggaa tattatccaa gacgcttttg acaacatctg
     5701 ctccaaataa aacggcaaag aatggaataa gagcataaag cgcatagtgg acaaaccaca
     5761 taaagaagtg cagattgatg attccttttt gatcaccaga ttttgcaaat ttgaaaaatt
     5821 tagcagccat cggtccacat acaaaagaaa aaatcatatt cattaactgt aatcctaata
     5881 ctgctagtgg aaagacaatg ggaataatcg catccacgtt tcctcccatt ttgattgcga
     5941 acgctgtagc cagcccagca gccaaagaag gctccgccgt actagagatt ccgatattga
     6001 cgacacccat aaagatggct tctagtgacg ccccgacaat caaccccgtc tgcaaatccc
     6061 ccatcaacgc tcctgctaac ggagcaacaa caatcggacg actcagcatg gacatcccga
     6121 acatttcatg tccagcaact ccaataaaaa cggccaaagc gactacaaat gcatcataca
     6181 taatttttct ccttcctttt tctaaaaatc actcaaaata gagatagctg aacgtttttc
     6241 catcgccggt gtaggctgaa aaacggtatc tggataaagt tcaactaatt cttttaagtc
     6301 ttgaatttct gacgtcgtta acatcacttc ctttgataag gttttcttat cttccgatgg
     6361 cggtgtcatt ttcccagcat tccccacgtt cattaattcg atttccgttg ggaaagcttt
     6421 cgcaactttt accgcatctt tgacattagg gactaaaacc atcaagcgca tgtccttcgc
     6481 ccgcggatct tttaacaaat gaatcgcgtc atcaactgtt ttaataatga ctttgacatt
     6541 cgatggtgca gccatcttca atgtcatctt ttgaatatcg ttggtagccg cctcatcgct
     6601 tgctactact aacccggtta aatttagact ctttgtccaa gtcatcgcta cttgtccatg
     6661 aattaatcgt tcatcaattc gcactgcttt aatcattttt cttcctccta aaaaaaatct
     6721 tcctcttcat ttactacatg tacctcatca agaatggtca tctttaattc ctgttggcat
     6781 ttatttacca gttcctgaat atcctccttt gttaattcct tggttgccaa tactatttcg
     6841 agcagaattg gcagattaaa gccagacaaa atagttacag caatctcttt ctccaaaaag
     6901 gttttggtaa tcttctgatt gacacttccg ccaaataaat cggtaaaaac aaaaatctga
     6961 tcctgctcgt tcacctggtt tacaaataca ttcaactcct gttcaaaatc acactcatcc
     7021 acataagcat taatcaccgt aatctcttga accttccccg tcagtaattg aatactgcta
     7081 agaaagcctg aagctaagtg accgtgagtc gctattagaa ttttcttcat atggttaatt
     7141 cctttctttc tgttcacttt tatataagca attcctgtgc caacttttaa aaacattggt
     7201 ttaatcacgt taattcacat taaaaaaagt gtctaaccta aaaaaaacag attagacact
     7261 tttttagaca ctttattttt gaatatacgg tcctaataat tcatagagca gcgaaagctc
     7321 ataagcattg actttaaaat tatatttcat ctccattgat tgaaaaatac ttctagaaac
     7381 ataataaaat tcatcttcat ctggtttcaa atcataattc ggttcttcag atttttctgt
     7441 ccgagccaag aataaacgtt ccatcatcaa tgccgtatgc atatagagat ttagttttac
     7501 acggccattt aaagtgaaat gatagtaatt ttcatattta gaaatgactg tttccacttc
     7561 actcatcacg atcgttgggt tcagaaagtt caaacggtcc gcaattcctt ccaacgaaaa
     7621 gaattttact agctcctgaa tcaagcggtt aaattctgcc ggcttcattt caggactcaa
     7681 aatatgctcc aaattggctg ctccatcacc gtctaagatg tcataaacat taatattagg
     7741 aattgaaaag gaacgtggaa gatccgaagt cgtgatgacc aattttgtct tgtcaaaata
     7801 agtttcatca ttttggtcga ttaattcacg tagttctctg tagtccttcg tgaaaacttc
     7861 gatggattca tccgataaat gatgacgaaa aacatctttc aatttgtccg aaatccccaa
     7921 acccgacata caggaaatga taatgttgct actttgagaa aagccttcaa agtattgaac
     7981 attgattgaa tacttgtctg ctgcatcctc cgcaatctgt ttaaatggcg cattcatcgt
     8041 catcttcaag ccaatatcaa gtgcaatgga agtcgtcaaa ttattcagaa gaagtaactc
     8101 cccttttaaa tcgttcttga tctgtgtgta aatttgattt aaagagccca tatctacgat
     8161 cagcactaga ctatctgtat tcacttgccg tttcacgaaa ttccttgtcg attcgacaat
     8221 ctctgacaca ttactctcta ctggcatatc caacgcttca aaaacataat tgccgcataa
     8281 ttgattcact actgcttgaa tgctgctagc cgtactatct ccgtgagcaa gcaacaggcc
     8341 cttcagctcg atggattcat ctacataatc agataaagtc acgacaataa gcagtcttag
     8401 aaagttctcg tccttttctg agagcggtgg taaatgctcc aagaactgat tacataagta
     8461 agtcgtccga ggtagatttg cagctaatgt acgatttatc ccggttaagt ctgtcttata
     8521 gcttggggaa atgacatagg atctgctaat aatttctgca acaggtccac tcgtcgtcaa
     8581 tccgtacgtt tttcctaaaa cggataccca gacctttcta aacatctgaa tcatcaattt
     8641 tctactcatt gataaaaaag aatccttatc aatgtgatgc accaaatgct gtacttcatt
     8701 ctttatcgaa gaaaaatttt cattgaaatt accttcattt atcagctgag taattttttc
     8761 caaaatcgaa tcataatcaa acggtttgat tcgatccgtt tttatctttt gatttttctt
     8821 tatcaataaa ctagcaatcg gcaagctttc tacttcggat tccagcatat ttaactgaat
     8881 caagatctca tttttcccaa tcgttaatgg ttcttcttgt gttcgactat acacatccgc
     8941 acaactgatt ttaatgatat tcttcagctt accaatattc ccactaaagg gatgagataa
     9001 cattaaagac aaggcttctc ttgaaacaat aatatctttt tgtaaaacat ccgcttcatt
     9061 ttgaaaaaat aattgaatca gctctaatcg ttctcgaatg gggcgatctt caaaagttgg
     9121 caacataaca ctaatttgaa tacgccgatc aaaggtgtct aataaataat tttcaccttt
     9181 ttccgtcgtg gcgaaaataa aacgaacctt agcagaatgc caatttacat tttcaccgac
     9241 tggacgatag tttccagtat caataaatag aaagagcttt tcttgatttt ctttcgatag
     9301 gcgatggacc tcatcgagaa aaagatagcc gttgttggcc gttgccaata aaccatcggt
     9361 atccttttca gcccccgtaa aggccccctt cttgtatcca aacagtgttg ccgaaagcag
     9421 ttcgggattg tccgcataat cggcacagtt taagatagca aatggtgcat ctttttcaat
     9481 aatttgctca tggatagcgt attcaaaaat ttttgtggct aaaaaacttt ttccaacacc
     9541 cgtggctcca tttattaata cactgagtcc gttaggcgga tatttgacag cggctataca
     9601 tttttgaatg acctctctca gacttccgtc atgaccaact acagaatgaa aggcttcgtt
     9661 tatttgctcc gtattatcta caaccgcata ttcatctaaa ggcttccaca aaacaggctt
     9721 tcccggtaat ttctcgacct ttttatcttc cactaatctc gtcaaataat ggctggcatt
     9781 ctgacgagag atacctagat tcttcgaaat tttctcggca gtgacagaag acatattccc
     9841 tgttagtcgt atcagctcat caaaaactcg atctttagcg ctctttttca ttttttcctc
     9901 cagtttaatg tgtctaaaag cataataaac tatcggctgc aatatgtcta ttctatattt
     9961 gtattccaat ttttactttg aaacacaaat agtcgaatat ttgcctcaga tatctgacaa
    10021 aataatttat ctaccaattt agtttttatc taatatgatt tttatgtttt ttcatagcta
    10081 caaatattga actggattca tatattagtt ttattaatag attacagaag ggttaagtat
    10141 atttgcatag tttccaatta ctttatcctt ctgtaacttt ttcaatctta gatttactag
    10201 cccgtctcgg gccctatttt acaatggttt tgtccacttt tctctggtag aaaaacgtgg
    10261 tagaaaaaat aaaataacac aaaaaaagcg gcgtttcatc cgcttttggt agaagattgg
    10321 aaaactcaga tagaaaaagg acgattttta gctctttaga acgttgataa gtccccattc
    10381 ttactgagtt ttgccattat ctcctataca tcttgaattc taagaatagg tcattataga
    10441 tgcctaggta tttcgatcct agatctttgt agacttcaag atgggagatc gtgtcatcag
    10501 atggatagaa ttgttcgtcc gacgaaatgt cttttggcaa taacgctttt gcttcttcat
    10561 ttggtgtaga ataaccaata tactctgcat tttgtgccgc attttccggt tctagcataa
    10621 agttgatgaa atcataggca ccttctttat ttttcacggt ttttggcata acgatattat
    10681 caaaccaaag attggatcct tctggtggga tcacataatg taaatgttca ttttcccaca
    10741 tcatatcggc agcttcgcca gaaaatgtca ctgccacgga ggcttcttca ttgatcatat
    10801 acattttgat ctcatctgcc acgattgctt tgacatttgg tgttaaatga ttcaatttat
    10861 ttgctgcttc tgtcagctgt gccatattct tactattcaa agaatagcct aaactattca
    10921 aagaaagccc cataacttct cgtgcaccgt caatcagcat caaactgtct tttaattccg
    10981 gcttccacaa atcatcccaa tgcttaattt gatttgcatc aaagaattta tcgttatata
    11041 cgatccctaa agttccccaa aaatatggaa tcgaatactg attatctgga tcaaaatcca
    11101 agtctaaaaa tcgtggatca atattattta atcctttgat cttcgaatga tctaatggaa
    11161 gtactagttt ttctttgatc attttttgga tcatatattc acttggtata gcgatgtcgt
    11221 agtttgtccc accttgctgg atttttgtga acatcgcttc attagaatca aatgtttcat
    11281 aattcacttt gtatccatat tctttttcaa atttcgtgat caaactagga tcgatataat
    11341 ctccccagtt atagatggta agtacctttg ctccactcat gccacttgtt ttttcaagtt
    11401 gacgaacacc aagcagcagc accgcaataa tgatgagaat acctaaaaac agagattgta
    11461 attttttcat cgcagccgtg ccaccccctc gcgtactctt ctgtttttct tcagccgttt
    11521 tggacggttt tctttactaa taaagtagta tccaatgacc aatatcatgg agaacaagaa
    11581 aactaacgca cttagcgcat tgatttctaa actgattcct tgtcttgcac gagaatagat
    11641 ctctactgag agcgtcgtaa agccatttcc tgtgacgaaa aatgtaaccg caaaatcatc
    11701 aagagaatag gtaaatgcca taaaatatcc agctatgatt cctggagaaa gataaggcaa
    11761 aatgatattt ttgactactt ggaaattatt agcaccaagg tctctagccg catcgaccat
    11821 tgaatcgttc atttcctgca attttggcaa gaccatcaag acgacgattg gaatgctgaa
    11881 agcaatatgg gaaagcaata cactctgaaa acctaatccg aatcctagta gtgtaaagaa
    11941 aatcaagaaa ctagcaccga taataacatc aggagagact aataagatgt tattcatgct
    12001 caacaacgct gtccgtgcac gtcgtttttt cgtgtaatag attcccatag ccccaaatgt
    12061 cccgatgatc gtggcgatca atgcagataa gaaagccagc atgaacgtat tcaatacgat
    12121 aatgatcaaa cgtgtatctt caaaaacagc ttcataattt tcccaagtaa atcctgtaaa
    12181 gctgttcatc gttcctcctt cattgaagga ataaaaaatc agatagaaga tcggtgcata
    12241 taagaataaa aagacgatcc ctaaataaag atacgaccat ttaaattttt tcatttatga
    12301 gcgacctcct tttttcttct cgcctgtcag gagcatcacg atgaacatag caatgattaa
    12361 aatcacaccg attgtagaac ccatccccca gttttgtgta accaagaaat gctgttcgat
    12421 agccgttcct aaagtgatca cccgatttcc gccaatcaga cgagtcagca tgaataaaga
    12481 aagagaagga ataaatactg cttggacacc acttttcaca ccatttaatg ataacggaaa
    12541 aatcactcgt cggaaggtct caaagctatt cgcgcctaaa tctctactag cactgatcaa
    12601 agaaggattc atctcttcca atgcattgaa aattggcata atcataaatg gaatctcgat
    12661 atacgttgcc acgaacagaa aactgaagtc tgtgaataat atctgtttcg gcccgatccc
    12721 gataaaatca aagaaatgat tcacgctgcc gtgcacactg aaaatcccaa taaatgcgta
    12781 agctttaagc agcagattca cccatgtagg caaaataatc agcatcagcc ataattgctt
    12841 gtgcttcagt tttgttaaaa aataagcagt cgggtaactg atgatcagcg tgaaaaacgt
    12901 gatcaaaaat gcataccata cagaattgat cgtcatagaa agcgatgtcc ccgacgtaaa
    12961 ataagataga taattattca gcgtgaattg cccattcata tcaaaaaaag attgataaat
    13021 gatcattaat acaggcgcga tcacgaataa accgagccac aaaatataag ggacggaata
    13081 gatcctgcgc attgttttca ttcttgtccc ccctattctt cgtagctgtc aagacgtgca
    13141 tcgaattctt cttcagactc gttgaaacgc atgacatgga tatcttctgg ctcaaaggcc
    13201 aatcctactt tgcttccttc tttagctttt ttcgttgaat ggaccatcca ttcattttgc
    13261 tgttcatcat aacagatgat ttcataatgg acaccacgaa acagctgagt atcgaccgtt
    13321 acgactagtt ttcctttatc tggtgtcgtg atcgtcaaat cctctgggcg taaaacaact
    13381 tcaactggtt catttagacg catcccgcca tcgacacatt caaattgctt accaacaaac
    13441 tcaactagat tatcttcgat cattacaccg tccacgatat tgctctcccc aacaaaatca
    13501 gcaacgaaat gattgattgg ctcatcataa atatctacgg gtgtaccact ttggacgatt
    13561 ttccctttat tcatgacaaa aatctcatca ctcattgcca acgcttcttc ttggtcatga
    13621 gtaacaaaaa taaacgtgat tcctaatcgt tgttgcagat cccgcagttc atactgcata
    13681 tctgtacgta gtttcaaatc aagcgccgat aacggttcat ctaatagcaa tactttcggt
    13741 tcatttacaa ttgctcgtgc aatcgctaca cgctgtcttt gaccaccaga catttcacta
    13801 atctcccgtt tttcgtatcc aggaagctgg accatacgca aagcatcttt taccttcttt
    13861 tcgatatctg atttggacat tttcttgatc ttcaatccaa aagcaacgtt atcaaaaaca
    13921 ttcatatgag gaaaaagtgc ataatcttgg aagactgtat ttacttgtcg cttattagct
    13981 ggcaaatcat tcaaccgttt cccttcaaaa tagatgtccc cttctgtagc ttcggtgaat
    14041 ccagctataa tacgtaggat cgttgtttta ccacagccgg aaggaccaag cagtgtataa
    14101 aattttcctt gttcgatctc aaaacttact ttttttaata ctgtttcatc atcatagcgt
    14161 ttcactactt gatcaaatga aatgattggt ttccccacgt ttttaccccc tatattacaa
    14221 atatgattgc gttgcaacaa tcaatacttt tgttcgtttt ttgagatgat tccttaattg
    14281 atgttgtttt gtcgcttgat aatagatcga ctgtcctgct tttgccacat agaccgtctc
    14341 tcctaagctt aactctaatt ccccttctaa tacaaaaata aacgtttcag ataatgaagg
    14401 ttcaaacgtc ttatattctc ctgcaccttc aaaagtcagc aagacaggct ccatttcttt
    14461 ttcattcgaa tcagccacga gccattccaa ttcatacccg ttttcttcat cgaaataaac
    14521 cgtatgatct tcttttgaat aaaccacttg cagattcgct gcttcttgat gaaaaaattc
    14581 ttctggagtc acacctaaga cttcaagaat agaaaagaat gtttccattg atggtgaact
    14641 taaatctcgt tccaactggg aaatataccc ttttgacaaa tctgtgcgtt cgcctagttc
    14701 ttcttgtgtc aaattctttt ggatgcgcaa atttcttaac ttctcaccaa tttccataat
    14761 ttcctccgaa tcgaagtttt ctaatagtaa acttcctgtt tactaccact cgtattagta
    14821 tacgaaattt attgaaaaag gcaacccttt gtaaaatttt ttttgctgaa tatttcttta
    14881 tcttttggaa taaattctag ttgtttgatt ctttattttt cacctaattt ttgtctaaat
    14941 ttacggaaga acaagaataa aagagtaggc tttatggtat gataaaagaa aattttaaaa
    15001 ggagtgtctc acgaatgaac gcttttcgat cttttcaggt aataaaggaa cctgttttcc
    15061 atacagcaat cagtgatctg ccatttactg agttggctga aaatgaagtc ttggtaaaaa
    15121 ttgcctattc tgatgtcaat tataaagatg cattggcaat gagcgagtct ggtcaggtga
    15181 tacgaacata tccgatgact ccagggattg atttatccgg aactgtcgtc caaagtaaca
    15241 atccacgttt ttcaaaagaa gatcttgtac ttgctactgg ttttgggctt ggcgtcaccc
    15301 atccaggcgg ttatagccaa tatcaaaaag taccaggaga ctggttagtt cctctaccaa
    15361 aaaatatgac gctgcgtcaa gcgatgatcc ttggtacagc agggtttact gctatgttgt
    15421 gtgtgaacgc tttaatccgt caaggtatga cctccgataa aaaagttgtc gttactggtg
    15481 cttctggcgg tgtaggcagt acagcgatag ccctgttaca taaactagga ttccagtcaa
    15541 tcattgcctt ttccagaaaa gaagagtctg tcacatggct aaaaagtcta ggtgctttgc
    15601 aagtcgtacg acctgatgaa tttctgccag agacaacaaa agctttagga aaacagcaaa
    15661 tcgactatgt catcgacaca gtaggtggcg agcagctaag cagtcttctt ccattgatct
    15721 cttataacgg tgcagtagct ttatgtggca atgctggggg aatcaaacta aacgcgactg
    15781 tcttgccttt tattctgcga aacatccagt tgattggaat tgattctgtc aatgtaccaa
    15841 tagatcaacg tctttcttta tggcaacaga tggcggattt gcagattgca gatgaactgg
    15901 tcgtccaaga gattactctg gatcaattgc ctgaaacagc atctaaacta cttgctggaa
    15961 cacaccaagg aagaacacta gtaaatgtag gtgaccataa atgaagattc taatcacagc
    16021 tggaggaacg actgaaaaaa tcgatcaagt acgtgctatt acaaatcatt cgacaggacg
    16081 attagggcaa gcattagctg attatttagc tgccaatccg gataccacaa tcgattacgt
    16141 gactacgaaa caagcactga aaccgaaaag acactccaat ataacgattt atacgatcga
    16201 atctgcactg gacttatttt tacaattaga agaactaacg aaaaaagaac actacgatgc
    16261 gattatccac agtatggcag tcagtgattt tactcctgct tttagttttt cagaagaaca
    16321 gctggcaaaa aacctaccca cttcttcaac gcaagaagaa ctggacaatt ggtttgcgga
    16381 aaatgaacag acagatacca ctgtttcaaa gatctcttct aacaccgagc atcttgtgct
    16441 cgttttgaag aaaacaccaa aaatcatttc ttatctacgt gaatggcagc caaaagcaaa
    16501 aatcattggt ttcaaactat tagtagatgt accaaaggaa tctcttctgg cagtcgcaaa
    16561 aaatagtctg ataaataaca aaactgattt tattttcgca aatgatttga cggaaatcca
    16621 tggcgagaca caccacggct atctcttatc taaagatgga acagtggaag aagctcaaag
    16681 taaatctgag attgctgcat tgattactga gaaaattaga ctggaggaat caaaatgaaa
    16741 aagatcttac taggcgttac tggaagtatt tctgcttaca aaagcgctga tcttacaagt
    16801 caattcgtca aattaggtgc ccaggtagat gtgattatgt ctaaaagcag cacacatttt
    16861 atcacgccat taaccttgca atctctgtcg aagcgcgcag tccatacaga cgtcatgcaa
    16921 gaatcaaatc cgcaggtaat caatcatatc gaactagcaa aacaagctga cttgtttctc
    16981 atcgcgcctg ctacagcaaa cattatcggc aagctagcaa acggcattgc agacgatctt
    17041 ctttcaacag tcgcaatggc gcttccagtg ggaacaccaa aattgattgc ccctgccatg
    17101 aacacgaata tgtatcaaaa cccgatcact cagcgtaatc tggaaacatt gagatctgtc
    17161 ggctatcagg aaattgaacc aagagaatct cttttagctt gtggagattt tggaaaaggt
    17221 gccttagctg atattcagac gatcatcgaa caggtcatgc tgactcttaa tgaaagaaat
    17281 gcttagaaaa aaggagtact tatgaaaaat acaaaaaact ttacattaac tgcgatgttt
    17341 ttagctattt tgattttatt agctgttacg cctttaggtt ttatccctat cggtccaatc
    17401 aatgcaacaa ccatgcatat tcctgtaatc attgcttcga tcgttttagg accaagacta
    17461 ggtgcttttc ttggcggtac atttggactg atcagcatga tccgtagcac agttatccaa
    17521 acacctttgt cttttgtctt ttctccgttt atcccagtca tcggaacaga acatggaagc
    17581 tggaaagcct tgctgatcgc gtttattcct cgcatcttga ttggggtggt tccttatttc
    17641 gtttataaag gtctgcgcaa actaatgaaa caaaaagcag attccgtttc tttattctta
    17701 gctggacttt ccggttcatt gaccaatacg attttagtga tgaatatgat ctacttttta
    17761 ttccagcaag attatgccaa agtcatcgga acaaatttaa atgctgttta ttcagctatt
    17821 ctagcggtta tttttacaag tggtatacct gaaggaatcg tcgcaggtct tgcgactgca
    17881 gcagtatgca atgtcttgtt aagatacttc aagcaccctt ctcttactaa ctgatagtca
    17941 tcgtagccga tctctgattt ttttctcttt tggtgttact attgaatgga aagatttgac
    18001 gtcgaaagga tgatccaaat gaaaattttt gttgtaggtg caaacggaca aattggccga
    18061 cacctgataa aagatttagc tccaagttcc catgaaattt ttgcaggggt gagagatgtt
    18121 gcttcccaaa cattagtaaa aaaagagaat gtttcgtatg tctcttttga tttaacttgg
    18181 tccgtggaaa aaatgaccga agcattcaaa ggcatcgatg tattgatttt tgctgccggc
    18241 tctcaaggaa agaatcttct gcaagtagac ttggatggcg caatcaaaac agtgatcgca
    18301 gctgaaaatg cgcatgtctc tcgctatctg atggtcagtg ctgtttatgc agatgaacca
    18361 gcaaaatggc ctgagtccat gacagattat tatattacta aacattatgc agatgaatgg
    18421 ctgaaacgaa ctaacttaga ttttgtcatt cttcaaccag tcacattgac taacgatgaa
    18481 gaagttactt ccattcaatt aactaagcct aatgagaagg catcaaaaac gatcactcgc
    18541 tctacagttg ctgctgtact tgccgctcta gtggaagaaa cggatatttc tcgtacgact
    18601 ctagtacttt ctgaaggtag caaagagttg aatacagcat tccaagaatg ggcaaaggag
    18661 gaatagatcc atgcgtgcca tttcaatcaa gcatcctggt tctgtagatg tattagaaca
    18721 aattgaagtg cctgttccta aaccaaaaca aggcgaatta ctgatcaaag tccatacagc
    18781 agctgtcaat cgaacagata ttatgaaaag agaatcagtc tctttgaaac ctccctaccc
    18841 tattttggga gtcgaagtag ctggagaggt agttgagaat aaaagtgata tctccacttt
    18901 tactcctggg acaaaagtat acggattagt caatctaggt ggttatgccg aatatgctgt
    18961 gatgcctgcc gatcgtgcaa tccttctccc tgatacgcta gactatgttt ctgctgctgg
    19021 aatctcagaa gtttttttga ctgcttatca aacactttac tggcttggaa aattacaaga
    19081 aggcgaaact gtcttgatcc atgcaggagc aagtggtgtg ggaacagcag cgatccaatt
    19141 agctagacag ctttctcacg cgaaaatcat cgttactgca ggatcagaag aaaaactcgc
    19201 cttttgtaaa gaacttggtg cagatgaatt gatcaactat aaaaaacaag actttcaaga
    19261 agaggtacga aaaatcactg aaggtcgggg agtcgatgtt attttggatt tcatcggcgc
    19321 ttcttactgg gaaaagaatt tagcttctat agccgttgat ggacgttggg tcttgattgg
    19381 tatgctaggt gggaccatcg tgcctgagat agaccttggt gtattgattg ggaaacgtgt
    19441 ccaactgctt ggtacattat tgacacctag aagtgatcag tataaagcag agctcgcaca
    19501 aacttttatg caaaaagctg ggccttttct agaaaatggc aagattcggc caatcattga
    19561 ccgtacattc caattcagcg cagtcaaaca agcgcatgaa tatatggaag caaacaaaaa
    19621 catcggaaaa atcattttga agataactga ataaaatcat gctgatcaaa aaagaggctg
    19681 ggacagaagc gtttaactcc aagaaataag aagaaattca cgaaaattgc ttttcaaatt
    19741 tttgtgaatt tcagcttatt tccgaaggaa tttcttctgc ttccgctgtt tatacgtgtt
    19801 tagagcgtga gacaaaagtg ttctttactt ttg
//
LOCUS       DAXWLF010000036        19680 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXWLF010000036 DAXWLF010000000
VERSION     DAXWLF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.036"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            192..1505
                     /locus_tag="ACGE4U_001008"
     CDS             192..1505
                     /locus_tag="ACGE4U_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HFF8393190.1"
                     /translation="MATLHEKKVQFNSKLTISNTGGNLSTDSGLVLVKEFMESLNFSD
                     LSKQYLGIEDKRLYHIHDNFSLMEQLIYQNIAGYSTDSSANILKQDPIFKVILDKSRL
                     ASQSSISRFWDRISEENISQLQDLNQAMIDKVRSARNTTEMIFDLDSTHSDTYGDQEN
                     TDYNAHYRTNGYHPLVAFDGLTGDFLKAELRSGNVYTSNGVGTFMEPLFEHYNQVVPV
                     SNILVRGDSGFATPELYDLCEAYDSFFVIRLKANRNLSKIAESFIQIDDNHPWDKKEV
                     VYSSTSYQAKSWSKERRVCIKSTREADELLFRHEYIVTNYSNNVSAETVFRTYCKRGT
                     MENFIKEAKNGFYFDKTDSPSFLENHARMMVSLLAYNIVNFMRTLCFTQGSASMQVDT
                     IRLKLFKVAGKLVRTGRRLLLKLSSYHLHQGLFYQVLGNIQQLCW"
     gene            complement(1708..2076)
                     /locus_tag="ACGE4U_001009"
     CDS             complement(1708..2076)
                     /locus_tag="ACGE4U_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFF8393191.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            2247..3203
                     /locus_tag="ACGE4U_001010"
     CDS             2247..3203
                     /locus_tag="ACGE4U_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8393192.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            3276..3392
                     /locus_tag="ACGE4U_001011"
     CDS             3276..3392
                     /locus_tag="ACGE4U_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFF8393193.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(3447..5129)
                     /gene="recN"
                     /locus_tag="ACGE4U_001012"
     CDS             complement(3447..5129)
                     /gene="recN"
                     /locus_tag="ACGE4U_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFF8393194.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(5147..5596)
                     /locus_tag="ACGE4U_001013"
     CDS             complement(5147..5596)
                     /locus_tag="ACGE4U_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8393195.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(5703..6521)
                     /locus_tag="ACGE4U_001014"
     CDS             complement(5703..6521)
                     /locus_tag="ACGE4U_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFF8393196.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVASNAHEQFDHK"
     gene            complement(6530..7420)
                     /locus_tag="ACGE4U_001015"
     CDS             complement(6530..7420)
                     /locus_tag="ACGE4U_001015"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFF8393197.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(7422..7646)
                     /locus_tag="ACGE4U_001016"
     CDS             complement(7422..7646)
                     /locus_tag="ACGE4U_001016"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFF8393198.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(7651..8988)
                     /gene="xseA"
                     /locus_tag="ACGE4U_001017"
     CDS             complement(7651..8988)
                     /gene="xseA"
                     /locus_tag="ACGE4U_001017"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFF8393199.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(8989..9843)
                     /locus_tag="ACGE4U_001018"
     CDS             complement(8989..9843)
                     /locus_tag="ACGE4U_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFF8393200.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(9940..10389)
                     /gene="nusB"
                     /locus_tag="ACGE4U_001019"
     CDS             complement(9940..10389)
                     /gene="nusB"
                     /locus_tag="ACGE4U_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFF8393201.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(10382..10807)
                     /locus_tag="ACGE4U_001020"
     CDS             complement(10382..10807)
                     /locus_tag="ACGE4U_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8393202.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(11053..12117)
                     /locus_tag="ACGE4U_001021"
     CDS             complement(11053..12117)
                     /locus_tag="ACGE4U_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFF8393203.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(12289..12534)
                     /locus_tag="ACGE4U_001022"
     CDS             complement(12289..12534)
                     /locus_tag="ACGE4U_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8393204.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(12673..13047)
                     /locus_tag="ACGE4U_001023"
     CDS             complement(12673..13047)
                     /locus_tag="ACGE4U_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFF8393205.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(13130..14044)
                     /locus_tag="ACGE4U_001024"
     CDS             complement(13130..14044)
                     /locus_tag="ACGE4U_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8393206.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(14065..14868)
                     /locus_tag="ACGE4U_001025"
     CDS             complement(14065..14868)
                     /locus_tag="ACGE4U_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFF8393207.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(14901..15890)
                     /locus_tag="ACGE4U_001026"
     CDS             complement(14901..15890)
                     /locus_tag="ACGE4U_001026"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFF8393208.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(15993..16484)
                     /locus_tag="ACGE4U_001027"
     CDS             complement(15993..16484)
                     /locus_tag="ACGE4U_001027"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFF8393209.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(16675..19482)
                     /locus_tag="ACGE4U_001028"
     CDS             complement(16675..19482)
                     /locus_tag="ACGE4U_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8393210.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
BASE COUNT         5672 a         4320 c         3295 g         6393 t
ORIGIN      
        1 taaaataaga aataaattaa caaataataa ggacagacct gaataattca taaaatttca
       61 ggaaactaca aataaccttg ctgcaatagc atttttcact tgttcgataa gcaccctttg
      121 ggtgtacaaa aagaacccca atctgatatg gttaagttac cacactaaaa ccatagaaag
      181 gggctctctc aatggctaca ttacacgaaa aaaaggtaca attcaactct aaactgacga
      241 tttcaaacac aggtggaaat ttgtccaccg attctggatt ggttctcgtc aaagaattta
      301 tggagtctct taatttctcc gatctttcta agcaatactt gggaatagag gacaaacgtc
      361 tctatcatat ccatgacaat ttttctttga tggagcagtt gatttatcaa aatatcgctg
      421 gctattcgac cgattcctcc gcaaatatat tgaagcaaga ccctatattc aaggtgattt
      481 tggataagtc caggcttgcc tcgcagtctt cgatttcccg attctgggat cgcataagcg
      541 aagaaaatat ttctcagttg caagatctta accaagccat gattgataaa gtacggtcgg
      601 caagaaatac gacagaaatg atctttgact tggattcaac ccattctgat acttacggag
      661 atcaagaaaa cactgattac aatgctcatt atcgaaccaa tggataccat ccgttagtcg
      721 catttgatgg tttgacggga gatttcttaa aagcagaact tcgttctggc aatgtctaca
      781 cgtctaatgg cgttgggaca tttatggagc ctctttttga gcattataac caagtggttc
      841 ctgtcagcaa tattctcgtc cgtggagata gcgggttcgc tacaccggaa ctttacgatc
      901 tctgtgaagc ttatgacagc ttctttgtga tccgcttaaa ggcgaaccgt aacctttcaa
      961 aaattgcgga aagctttatt cagattgacg ataaccaccc ttgggataaa aaagaagttg
     1021 tttattcttc aacatcctat caagcaaaaa gttggtccaa agaacgccga gtctgcatta
     1081 aatcaacacg ggaagctgat gaacttctat tccgacatga atacatcgtc accaattact
     1141 ccaataatgt ctctgcggaa acagtctttc ggacttactg taaacgcggc accatggaaa
     1201 acttcattaa agaagcgaag aatggctttt atttcgataa gaccgatagc ccttcctttt
     1261 tagaaaatca cgctcgcatg atggtaagcc tgttggctta caacatcgtt aactttatgc
     1321 gtacgctttg tttcacacag ggatcggcca gcatgcaggt ggacacaatc cgattaaaac
     1381 tattcaaggt cgcaggtaaa ctcgttcgaa caggacgtag actgctgctg aaactcagtt
     1441 cttatcattt acatcagggc ttattttatc aagtcctcgg aaatatccag cagctctgct
     1501 ggtaaataaa tcccaatttt aaaaattata ttgtacttca agggacgagt gcgcccaaat
     1561 ttggctgaac cgcagctgtt tacctaaaca gttcggattt tttaatctgt tgggttctga
     1621 gacggaataa aaaatataaa ttccggtaaa atttagagca acagatcatt tttttatcgg
     1681 tatgaattat tcaggtttct atttttttca taggtaagaa agtatgttca caatgatcaa
     1741 gacagcgtac aagacgattg tcaacagaaa aaccaaacgc caagtcatct tgaaataacg
     1801 cggataaata atttcttcat aataatatgc ttggaaaaca gcaatacaaa ttcccattag
     1861 caaaacagaa ataaagaaaa acggtaagat agaaattgtc cctgtatttc tagacaatgc
     1921 atgcataccg ccccacaaaa aaggaatcgc caaatcaggg gcttttattt taaaacgacg
     1981 atttaacgaa aataatttta caagaaattg gcaaccaaac aaaacgatta ccgggtaaat
     2041 ataccaaaac aagataagtg gtgtgaatgt gcccatgaac gtaaactcct tttttatcgt
     2101 tgcttcaagt atactgtata tactttacac aactgtttca ttttatttaa atttatgcag
     2161 ttatattcta tatcttacaa acacattcca tgtattccaa ttccctatcc gttataataa
     2221 aagaaacagg caacaggagg aaaatcatga tcaaatattt tacattagat aatgagaaac
     2281 tgcgtaatac cggaaaggaa actgaccaaa cgatctggta ttgtgtagaa cgtcctaatc
     2341 aagaagagat tcatcagctg acacatcaat ttgaaattcc gtcagattac atcacttcca
     2401 tattggacga tgcagaaaat tcccgtacag aagaattcaa tcaatcaaag ttgacaaagc
     2461 cagcgttatt attgttacaa tatccttttg ctaaaacaag tcctagcggt tattttcaag
     2521 tggatacata cccgctctct attattttga cgcccaaaaa gaagcttatc acaattacga
     2581 atcatgaacc actgttttta aagaaagtct ttagccagac ctttccaaaa agtgatattt
     2641 cgcctaatct caatatcttc tttcaattca tttggcaggt cacactctct tacaacagtt
     2701 atttagctac gcttcaatct caatgcgata ctttggaagc caaacttcaa gtatccactg
     2761 aaaacaaaca actttatcaa atcatggata tccaaaaaag cctcgtttat ttcagagaag
     2821 cgacttctgc taatttagat gcattgaaag aactagcgca aaataaactt ttccaagaaa
     2881 atcatgcttt gaaaaatcat ttacgtgatg tattggtgga aacagagcag gcaatgacta
     2941 cttctcgcat tcaattaaaa ctagtagaac aaatgaatca gactttttca gcaatcgtat
     3001 cgaataatct gaataatatt atgaaaattt taacttcttt aacgattatt ttaactatac
     3061 cgacaatcgt cggcagtatc tatggaatga acattaagtt acctattgct gaaagggatg
     3121 atgcctttat atggctagtt ctattaacat tatttatttg tatcgtaact acctattatt
     3181 taaaaaaagg gaagttttta taaaaaagcc ctcttgtgga aatggaaaaa agattgtaca
     3241 cttgaatcaa ctaattaatg acgaggttga aaacaatgcg tgaaaaaaaa caaacaagta
     3301 cttttgctaa agtaacaaaa tttgtcattt ggacaatgct gattttaacg attggatctg
     3361 ttgtattgac tgctgtaatg agtgtcatgt aaaaataaaa tatagctatc agctgctgaa
     3421 aaggacctga accactgcga ggtggctcag gtcctttttg cgagtttcaa tagttctttt
     3481 gcatgttcga gtgtgagttt tgtaatctca cttccagcca gcatacgtgc aatttcttct
     3541 tctctttctt ttgttttcaa ctctgaaaca gaagtttgcg tacgtccatc aatcactgct
     3601 ttcacaatat aatattgata gtcggcgact gcagcaactt gaggcagatg agtgatgcac
     3661 aaaacttgag agtatttcga tatttgagaa attttatctg cgatggcctg tgcaactctt
     3721 ccgctcacac ctgtatccac ttcatcaaaa ataatacttg tcacaccttg ctcagaagaa
     3781 aaaatagttt tcaaagcaag caacatccga gacagttcgc caccagaagc gactttcacc
     3841 aaaggcttca gtggctctcc tggattggtc gtaatgtaaa attcgatttg gtcaaatcca
     3901 tcatgattca attgtctatt ttcttctttt aagaaacgta cctcaaactc agtgttttcc
     3961 atatacagac ttttcaattc atgcagaatg gatttttcca attcttttgc gattttttta
     4021 cgcgcttcat gaagatctgc ggcttgttgc tgaagtagaa gctctttttg ctctagttcc
     4081 tctttcattt tgtccaattg accttctgaa aaatcagaag aagctagctc ttcggtgatt
     4141 tcgtcatagt aagccaatat tgactcaatg gattcgccgt atttacgttt caactgtcgg
     4201 attaattcta aacgttgcgt aacttcttct aaacgactct catctaactc caataaatca
     4261 atctgacgac tcatatcgcc gattgcatct tgcagcaaat agtaagcact ttggatattt
     4321 tcataaattg cttcatactc cacatctaga tgggcgatcc cttgcagttc tgaaacggcc
     4381 aagccaacac catctacact gttttgttca ttattcgtca gtgctccgta acctgcagct
     4441 aatccatcag caattttttg ataattgcta agtttttctc tttcttcttt taatttctct
     4501 tcttctccga cttgcaaatc agcagcagct atttcttctt gctggaaatg gagcatatct
     4561 attctctgaa cataggattt ttcattttgc tgcacttttc gtaccctttt ttccagctca
     4621 cgataagaga tatattcttg ctgataactc tttttcttct gttggaaagc tacatctcca
     4681 aaacgatcca ataaagcaag atgtgattca ggctggagca gttcctgatg ttcgttttgt
     4741 ccttggatat ctactaaata actgccgatt cttctcagat tagctaatgt gataatatga
     4801 ccattcactc gacaaacatt ttttccagtt aaggacatat cccgtcgaac aatcagattg
     4861 tcctcatctg tttcgatccc taattcaacc atcagttcag agaatccttc ttgttttggt
     4921 agttcaaaca acccttcaag cacacatttt tctgctcctt gacgtatata atcgcttgaa
     4981 cctcttcctc ctgctaacag acctaaagca tcgataataa tggatttacc cgcaccagtc
     5041 tctccggtca gcgcggtcat tccttcgtga aaagaaagcc gcaattccgg aataatcgca
     5101 aagttagtaa tactgatttc ttgtagcatc acattcgctc ctcaacttaa aggtaatgca
     5161 ggaagtcttt cttcaataaa actgcctggt cttcagttcg tgtaatcatc aaaacattat
     5221 cgtcatcatt gataatcgaa aataattctt tcttgtaacg tttatcaatc aaatttgctg
     5281 ctgccgaagc atttcccggc aatgttttca aaataacaaa tttttccatt tggtcaactg
     5341 ccacgaaagc gtcttttaat agtttttcca atttagtatt tacatcttcg tttgtttcag
     5401 ctggtatgct ataacggtag ccgccatctg aagccggtac cttgatcagt ttcagttcct
     5461 ttatatctct ggaaatcgta gcttgagtaa cagatattcc tttgcttttc aggatttcaa
     5521 caaattcttc ctgctttcgg atatcttgct cactcaacaa tcgagtaatc aaacgatgcc
     5581 gatctttttt tctcattacg tcttcgctcc ttagcatatt cttcttaatc aatgatagct
     5641 tatttccttg gtataataaa gaagaatccg catacttctc acttgatttt cacagttaca
     5701 atttatttat ggtcaaactg ttcgtgtgca tttgaagcca cttctgccat attaatagct
     5761 gaattgattg ttccgctttc gggtactttc ctcaagtgag ccaagaactc gatatttcct
     5821 tctccccccg tgatcggaga ataacttaat tccagcacat catatccacc ttgtgccgca
     5881 aatgacaaaa tgtcttctaa aactttttga tgggtctgag ggtcacgaac aattccgttt
     5941 ttccctacag cttctctgcc tgcttcgaat tggggtttga ttaaagcaat cacttctccc
     6001 tcttctttta aaatagcatg taatggaggc agcatcagtt ttagtgaaat aaaagaaaca
     6061 tcgataacag caacttctgg aattcctaag gtaaagtctt cgggttttgc ataacgaaaa
     6121 ttcactcttt ccattacgac gactctagga tcttgtctta ttttccaagc tagctgattg
     6181 tagccaacgt ctaaagcata actcattttt gcgccatttt gcaaagctgc atcagtaaat
     6241 ccgccagtgg atgcaccaat atctaacaat gtttttcctt caacagatag atcaaatgct
     6301 ttgagtgctt tttctaattt taatccccct cgagaaacat aaggaagttt tttacctttg
     6361 actcttaatt cactactcac tggaattttt tctcccggtt tatcaaaccg ctcgtttttt
     6421 tcgttgtaaa tcaaaccagc catcactgaa cgtttggctt gttctcttgt ttcaaataat
     6481 ccttgtttga ccgctaatac atctacgcgt tctttttcca ttctttccct catttctcca
     6541 catcaaattg cttgatcatc tggcgcatca gcccgccatc aaaagaaaaa acgtcctctt
     6601 ctaatttgtc tatgatcttg ttggcggata acagctcaat ctccaacgcc tcacgtgtct
     6661 tttctaggcc taataaacgc gggtaagtat ttttttccat acgttcatct ttaccagctg
     6721 ttttccctaa tgttttcgtt gtaccaatca catcgagtag atcatctctt atctgaaaag
     6781 ctaaacctaa atgttcggct aatcgttgaa gtagcaataa gatttcttca ggttgcttcg
     6841 ctaatatgcc accagccagc aaggcataac gaatcaaatg ccctgtcttt ctttcatgaa
     6901 taaatgctaa ttcttctaaa gaaagctttt tcgattcgcc ctcgatatct gcagcttgcc
     6961 cagcaaccat accttgtgat cccgcacaaa cagctaattg ctgaagcagc agaagcttag
     7021 gagaattacc taagtgtgcc atactgatta gctggaatgc tcctgtaagc aaaccatctc
     7081 cggccaatat agctaatgct tcgccgaata ctttatgatt ggtaggtttt cctctacgaa
     7141 gatcatcatt gtccatcgca ggcaaatcat cgtggatcaa ggaatacgta tgcaccattt
     7201 ctaaagcagc tgctacttga tagacgggaa catcaatagg ctcatcaaag gaagcaacgg
     7261 ccgttaaaag taataaggga cgtattcttt tcccgcctgc tttcactgaa taactcatcg
     7321 catcatagag tcctctatcc gttgtatgct catctaaaaa agacaagatt tctttttcta
     7381 cagcaggtag atgaatagct gaaaagtcag ttaatctcat ctcagctttc ctcgtttccg
     7441 tcaaaatcaa cttcttcatt tgattctgtc atcattttcg tcaaggtctc ttctgcttta
     7501 gctaatgtat cttggcaatg tttgcttaat tccattcctc gtttaaacga atctagagct
     7561 gattctaatg gaacgtctcc ttgttctaac tgcatgacaa ttttttctaa ttcttgcagt
     7621 gactcttcaa aagttggatt ttccatattt ttattctcct ttgtctatct cttgtacttg
     7681 tcctttaacg attccatctt gataatggat cgtcagcaaa tccccttgat gaatctcggt
     7741 gacacttttt actacttgat catttaacgt cgtatagctg tagcctcttc ccatgatttt
     7801 caatggactc aacaaatcaa gagattgaac agcttgagca aaacgctgct gctgttgttt
     7861 catatattgt tggattttct cttctaagcg tttttctaga taagcagttt cttggcgagc
     7921 tgctttaact cgataaagcg gagccacttg ttccaattgg tggcttaatg cgatcgtttg
     7981 tttttctttc gcatgataaa tttgctgtgt agcttgatat aatcgctgtt tcaactggtc
     8041 taatttgatg gtctgacctt catacaatct ttcaggctgc cgaaatacat aggaacgtcg
     8101 agcacgctcg aaccgttctt gtttgcgctg gatctggcgt acaaacgcct gctgcaatct
     8161 agcttgccgt tcttgaatgc gcatcaactc ttctgacaaa acaggaacag ctaattctgc
     8221 agctgctgtt ggagtggcag cccgaacatc tgctactaaa tcagcgatcg tggtatctgt
     8281 ttcatgtcct acagatgaaa tcaccggcgt attagcttga taaatcgccc gagcaacccg
     8341 ctcttcatta aacggccata aatcctcaat cgaaccaccc ccacgaccga tgatcattgt
     8401 atcgaaattg cccatctctt ctactctctg gatattcctg acaacattgt ccgctgcttg
     8461 gtttccttgt acaactgttg ggaacaaaac caattggaca attggatatc ttcttttcac
     8521 cgtcgtgatg atatcgcgga taaccgctcc gcttggactg gtaaggaccg cgattcgctt
     8581 tgggaaacgt ggcagcattt ttttaggccc ttgaaaaagg ccttccttca tcagcttttc
     8641 ttttcgttct tccaattctt gatataaagc acctattcca tcaggctcca tgtgctctac
     8701 atagatttga taagaacctc ccgcttcata taatgaaata cgcccaacga caaggacttt
     8761 cattccttct tttggctgga atcgtaattt ttggaatgct cctttgaaca taatagcaga
     8821 tatttttgca tggtcatctt ttaagctgaa atattgatgg gcattggcac gtaaacggaa
     8881 attcgatatt tcgcctgtta aatatacgcg ttctaaataa ggatcggcat caaattttct
     8941 tttcaaatac ttcgttaatg tagtgacagt taaatattct tgtgccatct attcacctgc
     9001 tccaaaagct tcggcagatt ttacggtttg atacatcagc atcgtgatag tcattggccc
     9061 tacacccttt ggcacaggag tgatccagcc tgcaataggc tccacttcat caaatttcac
     9121 atcaccgatc agcttaccgt tagcatcacg attcatccct acatctatca ctacagcacc
     9181 tggttttacg aattcttttg tgacaaaatg tccacgtcct attgctacga ctaaaatatc
     9241 tgcttcttgt gccagttcag ccagattttc ggttttagaa tgagcaacag taacagttgc
     9301 atctgccatc aagagtagct gagccatcgg tttaccgacg atattgcttc gtccgataac
     9361 tactgctcgt ttacccgccg gatcgataga ataagcttca aacattttca tgatgccgta
     9421 tggagtacaa ggaatcttgt ccggtttacc cgcaaggagt ctgcctaaat tcatcggatg
     9481 aaaaccgtct acgtcttttt cagggcggat agctaacagg atcttttctt catctatatg
     9541 ctttggcaat ggtaattgga ccaatatacc atgaaaacta ggatcttgat tgtatttttc
     9601 aatttctgca agtagttcgt cttcagaaat cgtctcagga taacgctcta ccaatgaatg
     9661 gattccgatt tcttttgcta cacgttcttt atttcttaca tagatttggc tggctggatt
     9721 ttcccctacc aataaaacga ctaatccggg atggatacca ttatctttca atttttggat
     9781 tttttcagca atctctgcct gcattttttc agctaattct tttccgttaa tcaattctgc
     9841 catctaacca ctcccagata ttttttgtgt tttcattcta ccatagaatt ttcccgattt
     9901 ataacaggtt tatgatgatt catagaaaaa agctaggact tacgccccag cttctaattc
     9961 tttcaataca ttggataaaa caccgttgac gaatttacgt gaacggtcat cactaaatgt
    10021 ttttgccaac tcgattgctt catttaaagc aacagccgct ggtacatctg tcacataagt
    10081 catttcaaaa atcgcgatac gcaagatcac caaatccatt tttgagatcc gattgatgtg
    10141 ccaattgttt tttaaatgtt tttcaatcac tttatccaat tcatcttttt ttgcacagac
    10201 accgccaact agcgtatcaa gataaacagg aacaaactct gattcatctt cgttgatcat
    10261 gtcgcggtgg tctaattcga ttgcgttgaa aatcgcatct tcttttgtta ggtctgcatt
    10321 gaagtctaat ggaaataaag cctgcaatgc catttcccga atctcgtgtc ttgataattc
    10381 tttattcatt ctcttcctct tcgtcttcaa aaagttcttc gattgttggt tgttctaatt
    10441 tttcaggaac aacagctact acatggatat tgacttcaga taaatcgata tccgtcatga
    10501 aaaggacttg ctggctgacc cgttcctgca tttccattgc tactttagga actgaaacac
    10561 catattctag ataacagtaa agatctactt tcaagccatc ttcctcgttt gctaagtaaa
    10621 caccttttcc gtgagctgca cgtcctaata attcagtgac attgctggca aaagttccgc
    10681 gcattccgta tacgccttct actttagaag ctgcaatccc gataattact tcgatgactt
    10741 caggagcgat cacgatctct cctaaatctt ggtttgcgtt taaaatcaaa tttttttcat
    10801 ctgccattta tttattcctc cctttgccta agcacgagat acatccgaat tctctatgat
    10861 gcggcatagt gatcacgtgg taagaaaact gccgccgatc agctgcaaac tgctatgaat
    10921 tggagaaaac acccaacttt attatctctt acagcttaaa agttcttcta tagtgtatca
    10981 ttaaatcgag tgattttcta taacaaagtc tgtgaacact gatttttacg cttacttctt
    11041 ctattgatga aactataaga tgatcaattc ttttggtgaa cgagtcaaca cacgatttcc
    11101 atcttctgtg atcaacaagt catcctcaat acgtactcca ccgattcctg gtaagtagat
    11161 ccctggttca tcagtgatca catttcctgg tacaaattgc ttttctgcac gagaagaaac
    11221 gtttggtcct tcgtggatct ctaaaccgat cccatgtcct gtagaatgtc caaatgcttc
    11281 cccgtaacca tgttttgaaa tataatctct agcaactgca tctaattgga caccagtcat
    11341 tcctggtttt gcagcatcaa taactgctaa ttgagcatct aagactattt gataaatttc
    11401 ttttaatttt tccccaggat caccaatagc gaatgtacgt gtcatatcgg aaacgtaccc
    11461 ttcgtaatag caaccaaagt ccaatgtgat caagtcacct tgttcgatga tttttttgct
    11521 tgctacacca tgcggcatag ccgaacgcaa tccgctagca acgatcgtat caaaagaaac
    11581 acctgaagcg ccaagagaac gcatataaaa gtctagttga tttgcgactt caatctcagt
    11641 catgcccggt tggatcattt tcaggatgtg atcatatgcc atatccgcaa tatgacatgc
    11701 tttttcgata atagcgattt cttcttcatc tttcacttct ctgagttctt cgatcatctg
    11761 tgaaacaggt actaattccg catcgatgat ttcttctaaa accgaatatt ctaaaaatga
    11821 gactgttgtt tcctcaaaag ctaatgagtg cagttcttct ttgcgtacta agtttgctac
    11881 ctcatcaaaa ataggtccga catttttaat aatttcaaac ccttgagcct gcgctgccgc
    11941 ctgctcggta taacgaaaat cagtaataaa aaatgctttt tccaaagtaa taacagctaa
    12001 tcctgtcgta cctgtaaagt tcgttaagta acgaagattg tacggactgg taaccaaaaa
    12061 tgaatccaaa ttttcttctt gcattttttt gcgcaatttt tctacacgta gcatcataat
    12121 gaaactccct ctttctttgc acgctttttt ccattatagc aaacttttct tcgtacacga
    12181 cagttaaata aaaatttccg gaaaacttct ttttatttta aataaataaa gccaaaaagg
    12241 agtcacagtc tcgctgcaac tccttttagt ttactatttt gtaaaaactt atgatcgttt
    12301 tccaaagaaa aatgagacaa ctgctaccaa aatcactgcg cctaaaatag atggaataag
    12361 tgccatacct gcagcttttg gtccccattc gccgaaaatc gcttgtccaa tacttgaacc
    12421 gactagtcca gcaatgatat tcgttacaca gcccatcgaa tttcctttac tggtgatcgc
    12481 acctgcaatt gctccgatca cggctccaac aatcaatgtc cataaccaac tcataatata
    12541 acctccttct ttgctaatca attttagcat atgggattta aaggggacaa ataattggtt
    12601 ttgctatttc atattttctt ctgcaaagta aaaaaataaa gaaaacctcc tcttagaagt
    12661 tttctttatt ttttacttgc gtttaaacat actacgaata ccacgtttca ctacatcaaa
    12721 gaaactcaat gattgtacca ttctatcttc atctacatat tctctgatcg cacgaagcac
    12781 ttttttaggt tctttggaag aaaatgtgta tgtaccattt tttttcgttt gaatggcata
    12841 tcgaggaatc cattttccct ttagcatgac tgatgctagt acataatcca cttcttccca
    12901 tggaatttgg atatatttgc gtctatctcg cgcatggtaa aactcaaaac cattatcacc
    12961 tatcatgatt tgcccgtaat ctgtcagacc cgtaaacgaa gtagcatcaa ttaccaagtc
    13021 tacttttgta ttgattgatt gaaccatttt ctccctccag atatttgttt atgaacagta
    13081 tactatataa aaaaagctcg ggacaacgcc cgagctatat ttttatggat tataaaagtc
    13141 cgatgacgtg tccaacaaca ccgactacga ataaaccaag gatgataacg attggagaaa
    13201 cctttttctt aagcaaccac atacataaca atgttaatcc taatgcagct aaaccaggaa
    13261 tcaattggtc taagttgtct tgtaatgttg ttaccttttc aggagaaagg gctaagccat
    13321 tattaacttg ttcaaatgca ctcttgattc cttcaccacc tgaaggcaat ttatcccatt
    13381 cgatgtaagc acctttatct aattttacag ttgatacaac tggtaagaat ttgattgata
    13441 cccatctttg tacaagggca gctaatacga acatccctaa aattgaagca ccttttgtta
    13501 cgtcttgtaa caatccgcca gaaaggtcat cagtaatttt agaaccagct ttgtagccaa
    13561 attcttgtgt ataccacatg aatgcccaac gaataatgtt ccaacctaag aagaagatga
    13621 ttggtccaag gatatttcct cccatagcta atgaagcacc tagtgcacct agcattggac
    13681 gtactgtaaa ccagaacact gggtcaccaa caccagctaa aggacccatc atacctactt
    13741 taaccccttg aatcgctaca tcatcaacag gcgcgccatt cgcacgttct tcttccagag
    13801 ccaatgtaac ccctaaaatt ggagaagcaa tgtatgggtg tgtattaaag aattccaagt
    13861 gacgttttaa tgctgcagaa cgttcttctt ttgttttgta taattttttg attgctggaa
    13921 tcatagaaaa tgcccagcca ccgttttgca tacgttcgta gttccatgaa ccttgaatga
    13981 aggtagagcg ccacgcaaca gctaaacgat ctttttttga taatttgatt ttttcttctg
    14041 ccattttatt ttcctccttc aagattaata gtcattcaaa atgtcgccta aagggtcacc
    14101 agagttgctt ccgcctccat tatttgatga accacccatt ttagaaaggt tcaagtaaat
    14161 aagagctaat gcaacaccta aagcacctag agcaatcaat gttaattgag aaattgcagc
    14221 tacaacgaaa ccaataacga agaatggcca tacttctttt gtagccatca tgttgattac
    14281 caaagcgtaa cctacagcta cgaccattcc cccaccgata gccataccat ctgttaacca
    14341 tgctggcatt gattcaagga atgattgcac agtttgtgct gggataaata gcaacgctgc
    14401 tgctgggacc gcgatacgaa ccccttgtaa acatacagca atgatgtgcc acatttcaac
    14461 cttacggatg tttccttctt cagcagccgc atccatcaaa tgaacgattg gaacagcgat
    14521 tgtacgtacg atcattgtta agaacaaccc tgcaacagcc aaaggaacgg caatcgcgat
    14581 cgcagaagga acacctttaa caccttgtcc acctaaaact aaaataattg ctgatgctac
    14641 tgatgctaat gcagcatctg gtgctacagc ggcaccgata tttgcccaac ctaaagcgat
    14701 catctgaagc gtaccgccaa gtacgatacc agcttcgagg ttaccggtaa ctaaaccgat
    14761 caatgtacat gccactagtg gttgatgaaa ttgaaattca tcaagaatac cttccatacc
    14821 agctagaaag gcaactaata cgactaaaat tattgaaata atagacatga taagcctcct
    14881 ataaatttta atattattta ttacgctttt gctaattcag cttttgcttt tttcaaaata
    14941 tcatccatgt tatcgcgtga atcattaggt actttacgta catcgaattt cacacctttt
    15001 tgttcaagct tctcaaacgc ttcaacgtct tcttgtccca tagacaatac tttacttaca
    15061 acgactttac cgaccgaatg cgccattgac ccaacattta attctttgat gtctacgcca
    15121 ccttcaacag ctgtcaatac gtcttcagga ttttcaaata aaagcaatgc tttggtattt
    15181 ccaaaacgag ggtcttttgc tacttcgatc attttgctaa taggaataac atttgctttt
    15241 acacctggag gagcagcttg ttcgattagt ctcttacgca gatcatcctt tgatactgca
    15301 tctgaaacaa cgataatacg gtttggttgc actgcttttg tccaagctgt cgcaacttgt
    15361 ccatgtaaca gacgtgagtc aacacgtgct aatacatatt tgatttttcc gtctccgacc
    15421 acagttcctt ctggaagtgc tcctttaggt tgtgcgtctt caactgctgc tttcggtgct
    15481 tctgccggtt ctaattcttc cggttttact tttacaccgt cttttgctgt ttcaatgatg
    15541 tgtgctgcaa tttcatgagc agattccatt gaaaagcgtg atgcgtaggc ttcaatcaac
    15601 attggtaaat tcaaaccgct gacgattgcc catttatctt tgtgctcttc gaataatgtg
    15661 tttgattgat tgaacggtgt tccgccccat aaatcaacta agaataacac ttcgtcttga
    15721 ttatcaaaag aagcaaccgc ttcttccaat ttggctctca agtcatctgg accttcactt
    15781 ggtttcaaaa tcacggcttt gacgttttct tgttcgccaa agatcattga accggattgt
    15841 aagattcctt cagcaaattg tccatgactt gctaggataa tccctaccat atttatacct
    15901 cctatttact tttgttgtaa caaattcaaa aatattaata attgctaacc attacaacag
    15961 aattatttga ccttttccaa caaccattct tgctacacct ttttgatttt cgctagtagt
    16021 tccatcaaaa ggattttcct gtcagcaggt acttttcgaa tctctaactc cacttcttga
    16081 ctgttcaagt aacgaaagga atcaatatct tgttgattta ctgaaacaaa attggtaatc
    16141 attgatttgc cttcagtaaa actcataccg ccgatattca acgacttcaa ctgtacaccg
    16201 cagcggacaa ccgcttcaac atcttgggga ttggtgaata aaagcatcac ttttgctcgt
    16261 gataataatc cactatgata gatttgaatc attttctgca cggttattac atttgctgtg
    16321 atccccggcg gtgcagcttc ttttagtaga aactttctca atttatcttg agcgactaca
    16381 tcgctgatca caatgatacg ttcaacacct gtttgcttcg accaaactgt tgcgacttgc
    16441 ccgtggatta aacgatcatc tatacgtgcc aatcgaatat ccatctgcaa aactccttct
    16501 tcctcactac ttcacagaaa cccctatcat ttctgtgtga acaaccacct cctagcacac
    16561 actactataa gcaaaaagcg tgccaacttt tttacgatac actaattgaa tttaaaggat
    16621 tcttaaccaa tacacaaaaa cgatacacta taaaagtgta tcgtttttgt gtattcatgg
    16681 cacagtgtat tcttttttat ctaactgatt atggactaga cgtagcagat aatacatttc
    16741 ttctatcggc acttttactt ttatctgatc ttccaacaga acaactgagg ctgccaattg
    16801 gctataaaga ggatcattcg ttgtatttgt caattcgttt tcttccacag tcaatggttc
    16861 attccgaata atacgttcga tcattcctgc tgtgtgcatc actaggttga ttttgaatcc
    16921 atattcttca tcgccgattc ccgtcgtctg agagatttgt gtagaaaatt gccacattgg
    16981 atcgattaac ttactaccat taataaagat gaagttatca gagataaact caacacacgt
    17041 atttttagct gactcttcat ctagggagat gaattcagtt tcctccaatt ctgattccat
    17101 cagcaactga tccaaaatga cttcgatatt ttgatcaata aaacgttcta aaggaataaa
    17161 tggggcttgg atattcaaat cagtaatccc agtagtagct atgatctgat attctttttg
    17221 gataactggc agttcttctt tcacttccac aatcgacaaa gctagaactt ctaattctgt
    17281 ttcttgacgc ttgtttaccg ctcgttcgat caattctttg attctttgtg ctgtaccttc
    17341 gccagatgcg cagatagcca aaacagcttt tttcttatga ataggttttg cttcctgaac
    17401 agattctaca gaagatattt ctgcgtaacc tcgaaaattt ttcaatgatt catgcaacat
    17461 atctagatct gtccctaata ctgagacctt tcgtacagtt tccaaaacta gagaagttgt
    17521 taccatatca acagtacgta ctggaattcc agtgtctctt tgaatttgcg aattaaacga
    17581 cgcaagggac cccatatcta ctaagagtat gaccccgctt ccttcatcta cttcttgtac
    17641 attgcgttca attcggtcca atgctgtttt cggatccata tccaaaggca tgtctactgc
    17701 tcgtacgttc tctgcatcta acaactgttg gacaacttgg accatactgc ttgctgtgct
    17761 gtttccgtga gctgcaacga ctacaccgat tctgccgctt gcttgatttg cccgtaacga
    17821 gacgagtaga acggtcaagt aatagtcttc actttctgga atctccactt gaaaatacgt
    17881 ttcgatataa ctacgcactt ctttcgcaac agcatattca tctggaaaat caaaagccat
    17941 ctcacggatg ttatcattcg tatgccgttc ttctcctaag ttgatttttt tcaaaaaaga
    18001 actgatatgc aagctcatag cataaataaa gttttgctga aactcatatg gtaaagatct
    18061 tttgaccatt acataaatct gattggtcac ttctatgatt ttcggatcta caaattctgc
    18121 taatttgctg tctgcattaa aagaaaaacc atggtcttta tagaaagact tcagatggat
    18181 gttgatatct gtagaaatga aatgattgat tgcttcttga tccaaaccat ccgacttcag
    18241 taaagctgct ttatctccga tgatatcata tagattataa ggtaactcat aggagtccga
    18301 ttggattttc atgatcgtat catttggtgc tacagttatc tttggctcca atattttaga
    18361 aagctctgcc attgcactgc ggttgcttgc taattgaatc aatccgctac gaatcccttc
    18421 tgtcaaatca tcgattgtga tcgagatttc gggtgattgc atatgattta gaaatcctcg
    18481 tgcacatact aactggatat ttgacttcaa ttgtcctata tttccatagg tcacactgcc
    18541 gatcaatgct tttgctacat cttctgtcaa agaaatcttt cgctgcgtgc ggtttgcttc
    18601 gtgggcaatc atgattttta ctaaatcgac tttctcattt gcaggacgtt tttcaaaagc
    18661 cggaagtttg atattgattg ggatacgccg aacaaaagtt tctaatagag aagaacctgg
    18721 gtcttctgtg gtcgcaccta cgatccgtac atttgcttca tgggatttcg ttgtttcccc
    18781 aagacgactg taagttccat gatccataaa gtaaaagatc atttcttgac cttcaggcgg
    18841 caaccggtga atctcatcca aaaacagcat accgccatcc gcttgatcga taatccccat
    18901 tttttcttca tctgcgcctg taaaagcacc ttttgcgtac ccaaataaat gactcatcaa
    18961 aagctcaggg ttattggcat aatcagcaca attgaaaaca atcagttccg tttcctcatc
    19021 tattacatga tgcgcttttg caaaatgata catcgcgtgt gcaaaataag tctttcctga
    19081 tccggtaggt cctgtaatca aacaatttaa tcctcttggc ggatacaaaa tcgcagcttt
    19141 tgcctgttcc actgaatttt tcatgctgcc gttagctcca atgatttttg caaagatatc
    19201 cttagtatct atttggagtg tatttttttc ttcagagacc ggcgtttctt tgtaactgac
    19261 tacatgtttt gtaactggct ttacgtgcgt aacgagtgtt tgatatatgt aacgtactgg
    19321 tcgtccgtct gtctttatca gttttccttc gcgcactagc tgattcagat ctttactgct
    19381 atttgttcgc tgaatcccta acacttcagc aatttcctgt gttgttactc cttgatcata
    19441 ttctgccttt gttaaatgag cggttttttc tttgacatac atataaattc ggtcaatgcg
    19501 ttttgccaat ggtatcgctc cttttctata gactattgta cttaggcttc aaaacataat
    19561 actatgtatt tatttctgtt tcgtcaacta tttacctgat tttgttttgt tgataacacg
    19621 cggatagata gaaatgttca atttaaaaga acgaaaaatc ccgatacgat ttttataatg
//
LOCUS       DAXWLF010000037        19492 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXWLF010000037 DAXWLF010000000
VERSION     DAXWLF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.037"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(111..566)
                     /locus_tag="ACGE4U_001029"
     CDS             complement(111..566)
                     /locus_tag="ACGE4U_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393211.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="ACGE4U_001030"
     CDS             complement(720..1928)
                     /locus_tag="ACGE4U_001030"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFF8393212.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="ACGE4U_001031"
     CDS             complement(2040..2372)
                     /locus_tag="ACGE4U_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFF8393213.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="ACGE4U_001032"
     CDS             2520..3395
                     /locus_tag="ACGE4U_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8393214.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="ACGE4U_001033"
     CDS             complement(3477..4058)
                     /locus_tag="ACGE4U_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFF8393215.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="ACGE4U_001034"
     CDS             complement(4064..5323)
                     /locus_tag="ACGE4U_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8393216.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="ACGE4U_001035"
     CDS             complement(5325..6371)
                     /locus_tag="ACGE4U_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393217.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="ACGE4U_001036"
     CDS             complement(6606..6881)
                     /locus_tag="ACGE4U_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFF8393218.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="ACGE4U_001037"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="ACGE4U_001037"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFF8393219.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ACGE4U_001038"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ACGE4U_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFF8393220.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /gene="cmk"
                     /locus_tag="ACGE4U_001039"
     CDS             complement(9980..10660)
                     /gene="cmk"
                     /locus_tag="ACGE4U_001039"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFF8393221.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="ACGE4U_001040"
     CDS             complement(10709..11344)
                     /locus_tag="ACGE4U_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFF8393222.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="ACGE4U_001041"
     CDS             complement(11410..12813)
                     /locus_tag="ACGE4U_001041"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFF8393223.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="ACGE4U_001042"
     CDS             complement(12800..13831)
                     /locus_tag="ACGE4U_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8393224.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="ACGE4U_001043"
     CDS             13862..14083
                     /locus_tag="ACGE4U_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFF8393225.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="ACGE4U_001044"
     CDS             complement(14248..14985)
                     /locus_tag="ACGE4U_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFF8393226.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="ACGE4U_001045"
     CDS             complement(15138..16478)
                     /locus_tag="ACGE4U_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8393227.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="ACGE4U_001046"
     CDS             complement(16715..18238)
                     /locus_tag="ACGE4U_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFF8393228.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="ACGE4U_001047"
     CDS             complement(18616..19227)
                     /locus_tag="ACGE4U_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8393229.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         5548 a         4130 c         3291 g         6523 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc cgttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tt
//
LOCUS       DAXWLF010000038        19251 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXWLF010000038 DAXWLF010000000
VERSION     DAXWLF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.038"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(40..636)
                     /locus_tag="ACGE4U_001048"
     CDS             complement(40..636)
                     /locus_tag="ACGE4U_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFF8393230.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            986..1330
                     /locus_tag="ACGE4U_001049"
     CDS             986..1330
                     /locus_tag="ACGE4U_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFF8393231.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1331..1750
                     /locus_tag="ACGE4U_001050"
     CDS             1331..1750
                     /locus_tag="ACGE4U_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFF8393232.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1842..2192)
                     /locus_tag="ACGE4U_001051"
     CDS             complement(1842..2192)
                     /locus_tag="ACGE4U_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393233.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2158..3489)
                     /locus_tag="ACGE4U_001052"
     CDS             complement(2158..3489)
                     /locus_tag="ACGE4U_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFF8393234.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3744..4310
                     /locus_tag="ACGE4U_001053"
     CDS             3744..4310
                     /locus_tag="ACGE4U_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8393235.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4455..4542
                     /locus_tag="ACGE4U_001054"
     tRNA            4455..4542
                     /locus_tag="ACGE4U_001054"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4487..4489,aa:Ser,seq:cga)
     gene            4878..5852
                     /gene="bsh"
                     /locus_tag="ACGE4U_001055"
     CDS             4878..5852
                     /gene="bsh"
                     /locus_tag="ACGE4U_001055"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFF8393236.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6177..8072
                     /gene="asnB"
                     /locus_tag="ACGE4U_001056"
     CDS             6177..8072
                     /gene="asnB"
                     /locus_tag="ACGE4U_001056"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFF8393237.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8267..8713)
                     /locus_tag="ACGE4U_001057"
     CDS             complement(8267..8713)
                     /locus_tag="ACGE4U_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFF8393238.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8941..9090
                     /gene="rpmG"
                     /locus_tag="ACGE4U_001058"
     CDS             8941..9090
                     /gene="rpmG"
                     /locus_tag="ACGE4U_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8393239.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9178..9696
                     /locus_tag="ACGE4U_001059"
     CDS             9178..9696
                     /locus_tag="ACGE4U_001059"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFF8393240.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9773..10729)
                     /locus_tag="ACGE4U_001060"
     CDS             complement(9773..10729)
                     /locus_tag="ACGE4U_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8393241.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10895..11701
                     /locus_tag="ACGE4U_001061"
     CDS             10895..11701
                     /locus_tag="ACGE4U_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393242.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11698..12687
                     /locus_tag="ACGE4U_001062"
     CDS             11698..12687
                     /locus_tag="ACGE4U_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8393243.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12684..13688
                     /locus_tag="ACGE4U_001063"
     CDS             12684..13688
                     /locus_tag="ACGE4U_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8393244.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13820..14362
                     /locus_tag="ACGE4U_001064"
     CDS             13820..14362
                     /locus_tag="ACGE4U_001064"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFF8393245.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14382..15080
                     /locus_tag="ACGE4U_001065"
     CDS             14382..15080
                     /locus_tag="ACGE4U_001065"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFF8393246.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15101..15313
                     /locus_tag="ACGE4U_001066"
     CDS             15101..15313
                     /locus_tag="ACGE4U_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFF8393247.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15313..16275
                     /locus_tag="ACGE4U_001067"
     CDS             15313..16275
                     /locus_tag="ACGE4U_001067"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFF8393248.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16293..16688
                     /locus_tag="ACGE4U_001068"
     CDS             16293..16688
                     /locus_tag="ACGE4U_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8393249.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16851..17216
                     /locus_tag="ACGE4U_001069"
     CDS             16851..17216
                     /locus_tag="ACGE4U_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFF8393250.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17213..19024
                     /locus_tag="ACGE4U_001070"
     CDS             17213..19024
                     /locus_tag="ACGE4U_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFF8393251.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19086..>19251)
                     /locus_tag="ACGE4U_001071"
     CDS             complement(19086..>19251)
                     /locus_tag="ACGE4U_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFF8393252.1"
                     /translation="KKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENR
                     KKAMRKRVSR"
BASE COUNT         6224 a         3282 c         3927 g         5818 t
ORIGIN      
        1 taacattgtc aatcaatgac taattttaca aaataaatat tatccaagaa gaccacttag
       61 atattgacct ccataaatca aggccatact atataagaaa atcgacagtg gtttagcaat
      121 caaaatgatt agtgtatatc gtttcactga catgtttgtc aatccagcca tcaaacataa
      181 cgcatcgtca ggagcaactg gaagaaaaat agctagagca aacagcttct caaaacggtt
      241 ttgattatcc agccagccaa tatatttgtt atatgtctta tcgctaatca tggaaagtat
      301 gaatggcttt ccataacgac gccctagcag aaaaatgata atcgaaccaa tgcagattcc
      361 aatatagtta tagataaatc cagcataagg gccaaatatc aggacaccag ctgcagtact
      421 gatccctcca ggaatgattg gaataactac ttgcaggatt tggatgaaga caaaaataat
      481 cggtcctaaa atgatggaat cacctactaa tcctcttaac gcattcaaat ctttgaaaac
      541 acctaaatta ataaaataaa tcgtcagcgc gattgatagg cctaatccga taatggatat
      601 gaaatttatc agttttcgag aggcagcaat attcattatt catcaactcc ttagaggttc
      661 tttgttaact atactttact agaaaagcaa ctaactgcgc cattttttga tatgacagaa
      721 aacaaacaat tttgtaagtt catcataagt ataaaagatt gatctcaaaa aaacatccaa
      781 ctaaagactt atttaataac ttgttttttt taaaataaaa aaagcgttag caactatgct
      841 taaaaagcat atcgactgaa taaactaagc attggacagg ctgaaagact acatttaaaa
      901 tgaaatgcga agaaacaata gaaaataaag ttgccttaga accatagttg aatcacctaa
      961 cagtcgtgaa gggagaataa gagaaatggc agaaaaacaa accgcaggac gagatcaatt
     1021 aggagatttt gcaccaaaat ttgcagaatt aaatgatgat gttttatttg gacaagtatg
     1081 gtccagagaa gaattatctg ctcatcagcg tagtatcatc accatatctg cattaatttc
     1141 aggaggaaat tttgaacaat tgccagctca tctgaagatt ggcaagcaaa atggcattac
     1201 tagtgaagaa atagcagaaa tcattaccca tttagctttt tatgtagaat ggccaaaagc
     1261 ttggtcagct tttagtttag caaaagaaat ctatttagaa gaaaatagcg aagataagaa
     1321 agaggcgtaa atgatgacaa aacacgaaga aatacacaat ggcgtgattt ttccagcagg
     1381 tgaaaaaaac gaagcttacg ctcaagtatt taccgggcaa agctatctga atatgttagt
     1441 gaatgatccc gaagtttctg ttggtgtagg taatgtaact tttgaaccag gttgtcgcaa
     1501 tcactggcat gtccatacag acggctatca aatcttactt gtgaccggag gagaaggctg
     1561 gtatcaggaa gaaggaaagc cagcaagacg attaatggct ggagatgtcg tcgtaacgca
     1621 caaagggatt aaacattggc atggtgcgac aaaagacagt tggtttgaac atttggcgat
     1681 cactgctggt aattcagagt ttttagaacc tgtaacagat gaagtgtatg aaggattgga
     1741 aaatcaataa tgtcaagctt gaacagctcc tgaaagtaga ttttaatcga actttcagga
     1801 gctgcttttt tgattcagtc ttttataaat agaaaatagg gttaataata ggaatgagaa
     1861 gcacgtcgaa agatcttaat attgataatc ggctgggcat acatgatttc cacgtcatca
     1921 atcaaactac ctaacagatg ataatcttcc cgatcatgtc ggggattacc taatagttct
     1981 tgatagtaga tttccatact agggtcccga ggattgttca gcaaatcagc aagtacatct
     2041 aaatcttctg gctgagtttc tgccgtaccc tgtatctcta tgaaaagccc ttcctcagca
     2101 aattctactc caatacgtat ttcagttatt tcatgggaat ataaataagt aagaagctca
     2161 tcgatgattc tttttgataa ttctttttcc aagtgtcttt ttttcctttc tgacagcggt
     2221 aaagttatct agtatcggta caaggatacc tgcaacgaaa ccagttgaaa aaccactgtt
     2281 atatagattt agtcctccgt gcaaagtgct gacgttatgg acaagagcaa tatgtagcac
     2341 accagctatt acaccgtaaa aactgccgta aaaaccgctt atgggcgcaa gacctgtccc
     2401 aaacacagct gcaacaagca tgccagtact ggtgatctca tgaaaagttc caaacaaaga
     2461 agcaataaat attccgacta gtatcggaaa actgtttttt aaatgacaac caaaagcact
     2521 gaaacccacc gcagataaaa tagcaccgat gacaggtcca tttaactgac cgccaaccag
     2581 caaaacataa gaagacagca tcagaccaac taagcccatg ttgatcaaag tagcaccaat
     2641 accagaatta gcaatgaaat cagttgtcaa ttttccagat gaatcgaaaa tttctcgaat
     2701 ccccgaaagc cgaaaggaat tataatagaa accaattccg actgtcagcg aaaaaaagcc
     2761 aaaaataatc caaatgagag gaaagtgata ttcattgaaa accaatgtat tttcgacgat
     2821 tgaatagctg aataacctca aaacagcagt aaaagtcatc gcaatcaacc cagaggtgaa
     2881 tccaatatta taaagtgtga atccacgatg gaaaactaaa gtttgagcag ctaagggcgg
     2941 aagaagtata ccaattgcta tgccagctaa attccccaat aaaataccta tcagtaaagg
     3001 aaaacgcata ccaaaagtga tataactaac gaccggtgat aatacgctac caaacagtcc
     3061 gatcatgatg tactgagaaa aaggtttatt gacaaataca gcgtatagat acacacctaa
     3121 aatgattgag atagaattat aaaggttttt gccaaaaaaa gagaaaccgg taactgttag
     3181 aatcgaagca aataagggac cgtttaaaat caccttgtgc cgataagcta aaagcacaga
     3241 aagtaaggtc aaaatcccta catttatgaa tgcgcttccg aaaacaccta aagcaaaata
     3301 atctgtcaaa agattgcttg gcgaggtaag aatagttagc aatccactcc aattatcagt
     3361 aggagtatta gaaagcaagc caataaaaag aaaaaaaaca cctgtaaaac caagataaaa
     3421 atactttctt ttctgtgaaa cgattcgttt ttgaggatga atataaataa cgtcagtttc
     3481 gttttccatt acacaccttc tttttcattt tctttaacta taacttgaaa tgaaagattt
     3541 ggacaactat catttaggtc aaaactaaaa atgtaaggga acccattgaa tttaaccaaa
     3601 tttttttaat tttgcaaaaa aaagttaaag aagactgaaa aaaatttaaa atataacgtt
     3661 ttgagtaaaa tgtttgcaaa aaatgctaaa aatagggtac aataaaacaa catatactgc
     3721 agtaaagaag tgggggagtt ttaatgagaa gaaaagtcta taccaaggat caaattctaa
     3781 aagctgctta tgatgttgtt gcaaaggaag ggtttaaagg atttactgcg agaaatattg
     3841 ctaaaaagat ggggatttcg acacagccga tttatctgga attcaaaaac atggaagatt
     3901 taaaaaatac gttgctggat tctatctttg aacaactaaa agaagatgtt ttccctattg
     3961 aacgtactgg tgataaaatc attgatctcg gtttgaacta tatctacttt gcaaaaaagc
     4021 atcatagtct ttacatggca ttgtacattg atgaacatgg ggacggaaag aaaatgcatg
     4081 atttttcata caattatttc cgtgaaatag ttaaagaaga tgaaagatac aatcaattat
     4141 cggatgaata tgtcgatgct ctgcatactg gtacatgggt agcagtgact ggcttagcgt
     4201 cattgatgtc atcaaacatc atgcatccaa cagaagaaca aatcattgca ctgatcaaac
     4261 ggacaattga ctcgatttta gcattagata atcctgaaat aatcagatag tgttctatca
     4321 gtatgaattt gatcggtaag tccgttggct gcaaactgtc aatggacttt tttgctaaaa
     4381 aaagcgattt ctgttattta tctattagaa ataggcttga catttcgtga aaaaagttta
     4441 aaaatagtac ttacggaagg ttggcagagt ggtaatgcac cggactcgaa atccggcgaa
     4501 ccggctaata ccggcgcgca ggttcaaatc ctgtaccttc cttatatcaa cgattagagg
     4561 ttgtgaagaa gctgtcctgc atcaagtaat aagaacggcc aaacaaaaat agcttttcct
     4621 attttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttgttcg
     4681 gaataagggg ctgtgacaag acttttgtca cagcccctta ttgttgtact tttaacctac
     4741 aatatttttt gattccccct gagtactatt ttttataaat gctagtaaat caatcttctt
     4801 tggccttttt tttgataaaa aaaaatacta gatgtaagat aaaagtgtaa aaacgatttg
     4861 ttttaggagg gaaaacgatg tgtaccgcaa tcacttatgc aacgaaagac cattattttg
     4921 gaaggaattt tgattatgag atgtcttata atgaagtagt cactattact ccaagaaatt
     4981 atcgtttcga ttttagaaag gtaaaaaatt tagataaaca ttatgcaatg ataggaatag
     5041 cagctggggt atccaactat ccactctatt atgaggctac aaatgaaaaa gggttgagta
     5101 tggcaggact gaattttcct ggcaatgctg attataaaga attacaagaa ggaaaagata
     5161 acgttgcacc atttgagttt attccatgga tactagggca atgctcaacc atagacgaag
     5221 ctaaagaatt attggctact atcaatctag tcaatatcga ttttagtgaa aaactaccct
     5281 tgtcaccttt acactggtta ttagctgata aagaaaaatc gatagtaatt gaaagtatga
     5341 aagatggact tcatttatat gataatcctg tcggtgtgtt gacaaataat ccgccatttg
     5401 attatcaatt atttaatctg aacaattatc gctcgctatc aaatggaaca ccagaaaatc
     5461 atttttcaaa ccagatcagc ttgaatgttt atagtcgtgg gatgggagga ttgggcttac
     5521 ctggagatct ctcttctgtt tctcgatttg taaaagccac atttactaaa atgaatgctg
     5581 catcaggtga ttcagaatct gaaagtatca gtcaattttt tcatatacta ggctcagtcg
     5641 agcaacaaaa aggagtatgc gatactggag aaggaaaata cgaatatacg atttattctt
     5701 cttgctgtaa cgttgataaa gggatttatt attatcgtac gtatgaagac agtcaaatta
     5761 ctgctgtaaa tataaataaa gaagacttgg atcgtcatga attggtcagt tatccagtca
     5821 taaaagagca acagataaac tatgtcaatt aattaatgaa tagatccgtt tggtatcata
     5881 ttttctgata cagtcataat tagacacgat ctttaggtcg tgtctttttt tgctgttttt
     5941 tttggaaata gcatccacta atattttttt aacacgaaga gaataaggta gcctcaaaaa
     6001 agaaaacatg gtaaattaga tattgggact ttattttctg taaaaacaga aactttcatc
     6061 ttttgttttc acgtgacgaa attgtaagag agaaaatatt cgtccgctat ttattctatg
     6121 ataagatgca tatgaatgaa ataaacagta attttatatt aggatgtgaa agttaaatgt
     6181 gcggtattgt aggttttgtt aacgacaagg acaacaaaaa aacaattatt aatacaatga
     6241 tggatcgaat cgttcaccgt ggacctaata gttcaggaga atatatcgat aaacatgttg
     6301 ctttaggatt cagaagatta agtattattg acttagaggg cggtactcag cctatttaca
     6361 atgaagatcg gacaaagatc attattttta atggcgaaat ctataattac cagcctttga
     6421 gagaagaatt gattgcagct ggccacgttt ttcaaacaca tgcagataca gaagtccttt
     6481 tgcatggtta cgaagaatgg ggaacagaat tattgcaaaa gatccgcgga atgtttgctt
     6541 ttgctatttg ggataatgaa aagaatgagt tattcggtgc aagagaccac tttgggatca
     6601 aaccatatta ttatgcagaa atgaacggaa catttatgtt tggttcagaa atcaaaagtt
     6661 ttctgcctca tcctgatttt aacaaagaat tgaacaaaga agcgttgaaa ccttatatga
     6721 ctttccaata ttctccatta aacggcgaaa cattcttcaa agatgtctat cgtttgcctg
     6781 aaggccatta ttatacgtat aaagatggaa aactagacat tcagcaatat tgggatgcag
     6841 attttgaaac aaaagaaacg cattcccgac aagaatggat cgaaaaaatc gacgaaaccg
     6901 ttcaagcttc tattgaagca catacggtca gtgatgtaga agttggttct ttcttatcca
     6961 gcggggttga ttccagttat gtaacctctg tattgaaacc agatcattct ttctcaatcg
     7021 ggtttgatga taaaacctat aatgaagcga ttgaagccag aaaactaacc gaactattgg
     7081 acttagataa tacagcagca gtgatcgacg gagacatgtc gttcaaagca ttccctttga
     7141 tccagtatca cttagatgaa cctgattcaa atccttcttg cgtacctttg tactttttag
     7201 caaatcttgc ttcacaaagt gtgcgggtag tccaatctgg agaaggggcg gatgaactgt
     7261 ttgcgggata ccaaacgtat ggattccata cgaattcaaa attcatccgt gtgattgctc
     7321 aaggattgaa aaaattacca aaaggaacac gctataattt aggacgtaaa atcggaaaaa
     7381 tgaaaaattt ccatggacgg atccatttat atgaatcact tgctcctgca aaagaatatt
     7441 ttatcggtca tgctcgagtg ttcgaagaat cagaagcatc agaagtgtta acaccaaaat
     7501 atcaaacagc accatctgtg gatgaaatca tgactgttca ttacgagaaa acagaaggaa
     7561 tcaaagatga agtcaataag atgcaatatg tcgatcttca tcaatggatg ccaaaagata
     7621 tcttgttaaa ggcagataaa ctttctatgg caagttcttt ggaagtccga gtacctttat
     7681 tggatatcga agtcatgaaa ttggctcaac aaataccaag taagttttta ttgaatcaaa
     7741 acaacacaaa agatattttc agacaagctg cgaacaaaca tcttccggaa gaatggtcga
     7801 atcgtgtgaa attaggattc cctgtgccaa tcaaagcttg gttgaaagaa gaacacggct
     7861 acgaacaagt caaagcatta ttcgaagcag attttgcaaa agaatttttt gatcaagaaa
     7921 aaatcatgaa gttgctagat gaccatcacg aaggacgtaa agaagagcaa agaaaaatct
     7981 ggacaatctt cagtttcttg acatggtata aagtctactt tgtagatgaa acgattccgc
     8041 aagcagaagc aatcgactat gtgactgttt gatgaaaatc aaatatcaat aaaaaagagg
     8101 ttttctccta ccgtttaaca ctgagtgata agaaacaatt tcaaaaaaca gctcttcccg
     8161 tttcatagat attatgactt atggttcgga gttggcagag gaacaccatt gtttcgaaca
     8221 taagggggtt gtgacacgat tttcgtcaca acccctttac atatgttcaa taatcgattt
     8281 ctttttcttt tgtcccatct tttttataaa tgaacaaagt tgattctttg ttttgtgcta
     8341 cttgctttcc tttttcgatt gcttcttttt ttgtatcaaa atggttgctt gccttttttg
     8401 cccctttaga tttcacgatc cattgttctt cctcatattc tacgatcaca tctgaatcaa
     8461 gcagttttcc agcacgagga ttcgtatcgt gttcatccgt cttgcttgga tttttttctt
     8521 tttcgaaagt tcgtttttct gattcactag catcttcata ccattttttt gcttgatcaa
     8581 tggcaattgg gatcgcacgg ttatcagggt acccttcatc taataatgca tttgcgatgt
     8641 cgattgcttt ttttcttgtc aatttatcta agtttttcat tgatgcagga aagtccttca
     8701 tgttccatgg catattagta tcccccttta ttaaaaaaca taatctatat aaacaagtgt
     8761 aagggtattt atacaaaaag aaaaagattt tgtttgagaa gtaaaaccgt tgtatttatc
     8821 gtcaagtagt tttacttaac aaaatcgtga aaaacactag caatctagaa aaaatcatgg
     8881 taagataatc aagtctgaga gtcatcttag aggattcttg taaaaatggg agggaaaaac
     8941 atgcgcgtaa acatcacttt agagtgtact tcttgtaaag aacgtaacta tctaacaagc
     9001 aaaaacaaac gtaacaatcc tgatcgtttg gaaaaacaaa aatattgccc acgcgaacgc
     9061 aaagttactt tgcaccgtga aacaaaataa tgtttttaaa gttctcaatt ccttgctggt
     9121 caaagaattg agactttttt tatctcaaaa catctaaaat cataaggagc ggataatatg
     9181 tttatttcca tgtgggcaca agataaaaat ggtctgatcg gcaaaaatgg tttgttacca
     9241 tggcgattgc caaatgatat gcgtttcttt cgcgaacata caatggatcg aattttagtg
     9301 atgggaagaa agacctatga aggaatgggg gatctttcac ttccttatcg ccatatcatc
     9361 gtgttgacaa cgcaaccaaa ttttcgcacg aaagaaaatg cggaagtaat gcattcgatc
     9421 gatgaacttt tgtcgtatag tcaaacgatc tcagaagata tctatgtttc gggtggtagt
     9481 cggatttttc aagaacttct tccttacact ggtcggatct ggcgaacatt gatcgatagc
     9541 acgtttgaag gcgatacgta tattgggaat attgattttt cagacttcgc ccttatagag
     9601 gagtatgagg ggataacgga tcaagaaaat ctatatgccc atcgttttca aaaatgggaa
     9661 agagtaaaaa atagggttgc ttcaaaagaa gaataaaatc tatgcgtcta gtttgttaat
     9721 aagaaaaaaa gcatctcacg actaatcgtg aagattcttt ttttagttaa aatcaattta
     9781 gtttttcttt gatatcattg accatttcct gactggcaat aggtccgtta tatagccaac
     9841 tagattcagg tccgaattgc agtacatgat tgtctgtcac tgctttaaga ttcttccagc
     9901 ttggttcatt gaacatggca gcatctttgt cactgttcac aaggatgatg tagtcagcat
     9961 ctaattcagc tagcttttca gaggaaacag cagaccagtc agaagtggct tttttggaga
    10021 cctcttccac tagatcagga acaccaaatt ccagatcgtc gtatacgatt cttccacttg
    10081 accgattttt gctgaccata aaggctgagt tgttggtcac ccacaacact gcagctgtct
    10141 tgttctcgat tttatctgct agttcttctc gagttttctt gatggatttt tgataatcgg
    10201 aaatgatttt ttctgcctct ttttcttttg ccaaagcttt gccgacatct tttagttgtt
    10261 cttcccatgt gacatctgtt ccgtttttta caacataagt tggcgcgatt ttgctatatt
    10321 gttcgtattt cccgccttcg acagtagcgg aagaagaaat caacagcaaa tcaggctcaa
    10381 agcttaacac tttctcataa ggaaggtcat aagaaatcgt tgggacatcc tttaaatcat
    10441 cttgcagata atcttgaatg gaaccactgc caacagtcca ctgggcgata ggtttttccc
    10501 ctagtgcaat cagatagtct tctaagtaac tgccgatgat ccgtttgggc gcgtttggaa
    10561 tctctacttt atgtccaagt gtatctgtga ctgtatgagt actattggat gaagtcgtcg
    10621 aattggcagt tgtagctgtt tctttatctg aagaggggga gcaagcgctc agtactccag
    10681 ccagcagcaa tatgctagat aatgtgaata cttttgtttt taatttcatt tctttcctcc
    10741 taacataatt gagaatcttt ctcattggtt attattcctg atatttcgaa cgctgtcaac
    10801 cgcttcattt ggctatagat atgaaagtag cgcttgacga aatgttgaaa cagcgtcata
    10861 attgagaatg attttcagcg aaaggatggg tgaaatgacg agattaagag cagctgacat
    10921 tgcagccggc tacgaaaaca aaaaaatcat tgaaaacatg tctgttacga ttcctgaagg
    10981 gaaaatcact agtttgattg ggccaaatgg aagtggaaaa tctactttat tgaaaagttt
    11041 tacccgtata ctacctgtga aatcaggaaa gatcattgtc aataactctg atatctctac
    11101 atacagttca aaagaacttg caaaaaaaat tacattatta gcacaaacta gtgaacaccc
    11161 tacaggtatg acagtagaag aagtagtatc ttacggacga tacccttatc aaaaactgtt
    11221 cagtggcatg gacacgcaag atcatgaagc aatcgagtgg gcgttggatg ctacaggtct
    11281 agctggattt cgtatgcggg atatcgcaac gctgtctggt ggacaaaccc agcgtgtctg
    11341 gatcgctatg gcgcttgctc aggaaacaga tatcttgatc ttggatgaac cgacaacatt
    11401 tttagatccg gcacaccaat tagagatcct acagttattg actgaaatca atcaaatgaa
    11461 acaaacaacg atcttgatgt cgattcatga cattaatcat gcttcacgtt tttcagatta
    11521 tttattagca atgaaaaacg gtaagttgat cacagaaggt acacctgaag aagtgatcac
    11581 aaaagagtgt atgaaaaaaa tatttgagat tgatgcaacg attgtcacgc ttccaagaac
    11641 aaataaacca gtttttctaa cctatgattt gttgccaaaa gagcaggagg atcaattatg
    11701 aggactatga agaaaaatag actcctgctt ttggtctgtt ttgttctttt gattattttt
    11761 tttgttgctc tttcaaatgg cgcagcgact gtgggaaaag cagatttttg gcaggcgttg
    11821 tttgattttg atagatcaaa acaaagtcac caaatcatac gaaatattcg tttgcctcgt
    11881 gtggcgggag catttcttgt agggagttgt tttgcattga gcggtgcatt gatgcaagga
    11941 gtgacaagaa atcctttagc agattcaggc ttactaggaa tcaacgcggg tgcttcgttg
    12001 gcaatggccc ttagttttgc tttttggcct aaagcttctg atttttctgt gtttctaatg
    12061 tcattggcag gttcttttgc tgtaactttg ctcgtatttg gttttttgca gttttcgaaa
    12121 atgggcatga atcctttaca gctgatttta gctggcgtag ctattagttc tttttttact
    12181 gctatcagcc aagcacttac ccaattcttt aatctgcagc aggatcttgc tttttggttt
    12241 gtaggcggag ctgccaatat cacatggaaa caattagcag tattgtttcc tttatatata
    12301 cttgctgttt tggtttcttt ttcattagga aaagcaatat ctttgattca tttgagtgaa
    12361 acacatgcat tagctcttgg aagtcatcct aaacgaacgc ggttgattgt tttctgtcta
    12421 gttgcagtgt tagcagccat tgccgtttcg ttagtcggac ctgtttcatt tattgggctg
    12481 ttggttccgc cagttgttcg cggaatggtt ggaacggact atcgttatgt tctacctgtc
    12541 tcttttttag ttggcggtgg attggttatg ttggcggatt ttacagcgag gatgatcaat
    12601 cccccttttg aaacaccttt tggattagtc attgcattga ttggcgttcc gtttttactg
    12661 atacaagtaa ggagggaaca acggtgaaaa aaatatattg gacgttctat ttcttgcttt
    12721 ttgtacttgt catcctgtct ataggtagtt taatgattgg tacgcctttt atttacctgg
    12781 atcagctttt tgccacgttc atagggaaag aatcatcttc tttggcgctg attgttagag
    12841 aatttcgagt tcctcgatta ctgatcagtt tattagcagg agcttgtctt ggcgtttctg
    12901 gttatatctt gcaaggagta acaagaaatg aattggcaga ttctagtata ttgggcatta
    12961 atgccggagc aggattttta gtgatgcttt atttaggttt cttttcgcac tattcgctgc
    13021 cgtttctatt gctagttgta gcctttctag gtggtgtttt agctgcttgc tgtgtctatc
    13081 ttgcggccta tgatcgaaat ggctttttgg gtatgaatcg aatcttattg tccggtattg
    13141 ccgttaatgc cggcctttct gcattaacat tgctatgcac gatccaatta tctaaggaaa
    13201 attatggatt tgtgaatgca tggttagcgg gatcaatctg gggtgcgagc tggccttatg
    13261 tttgggcatt gcttccatgg gcggttatct taattccgct aggaggactt tatgcacaga
    13321 ggattggatt gctatcattt ggtcaagaac gcgcgcaagg attaggcctt aacgtggcta
    13381 aaagtcaaaa aatccttttg ttgttagctg ttgctttggc aagcggtagt gtagcggtga
    13441 caggtagttt atcttttgta ggactactag ctcctcatgc tgcgaagcta gtcgtacgta
    13501 aagaaaataa ctggaccttt gttctgagcg gtttgttcgg ttccattttt ctgcttagtg
    13561 cagatatggt tgctagagtg attctaccaa gtggggaagt acctacagga attttgattg
    13621 ccctttttgg tgcgccatat tttctgtatg cactttttat cagacaaaaa cacgcgctga
    13681 acagttgaaa tttattaaaa aaggaaggcc aatcatttct aggtcttctt tttattctat
    13741 ttatgcattt atttgaaagt tcgattgcta tttttctttt tttcctatac cattttatga
    13801 gagaaggaag ggtgagtttt tggaccaatt gaaagtaaga ttacgccgga aaggaatcga
    13861 aaagctgaaa aatctggcca aaagtggcaa gaaaaaggaa aaagaacagc gaatctataa
    13921 acagctattc gccagcgagt actggaagtc agcaaaagtg atagggacga ctttatcgaa
    13981 tgaatttgaa ctggatacac agccggttat ccaacgagca gaaaaagaac aaaagaaaat
    14041 agttgtacct aaaactttgc cgaaacgtca gatggcgttt tatgaaatgg atgaacagtc
    14101 cgttcttgta cgttcgagtt ttggtgttct tgagccacaa tcagagaagc tctatttgcc
    14161 ggagatgatc gatctcctca ttgtaccagg tattatctac aatagagccg gttatcgaat
    14221 cggttttggc ggtggttatt acgaccggta tttaaataat tttcataaaa aaacatgtag
    14281 ccttgttttt cgtgaacaat tgaatgaaca atggcaaccg gagagctttg atcagaagat
    14341 ccaacaattg tttattgact aaaatgaaat gggaggtttt catgaattat caacagcaaa
    14401 taaaattgcg tgcatggtta agacgaccgt tatggaccta tgctttttta gggatacaga
    14461 cagcggtctt cctcatgatg gaattatttc cgcgacttga gatcccttat tatgccggaa
    14521 tgtatggtcc ttatcttgtt cacttcaatg aatggtggcg tttagtgaca ccaatcttca
    14581 tccattttgg actgatgcat ttcgtaatga actcactgat actgtatttt atggggcagc
    14641 aaatcgaggc aatctatggc cattggcgtt ttttcttgat ttatatgttc agcgggataa
    14701 tgggaaatac agcaagcttt gcttttaacg aagcgaatgt tctgtctggt ggtgcgagta
    14761 cttcgatatt cggtttgttc ggtgcgttgt ttatcttagg gtttcatttt aaacataacc
    14821 cagcgatcca gcagctagtc agacattttc ttttatttat tgtgttgaca tttgtttttg
    14881 gtcttctgga tacctctgta gatgtttggg gacatgtcgg tgggctaatt ggcggattag
    14941 tgttagggaa tgttttgggt ctaccgaaac aacagacaag ctattcgata caccaaagga
    15001 ttctttctac actagttttt gtatttcttt ttgtaatatg tattttactg ggtttaaaaa
    15061 aatatggttt acttgtataa tatgaaggaa gtgcttaaag atggagagct tatatgatgt
    15121 acagcagctg ttcaaaaaat ttggcatctt tatctatgtg ggtgcacgca tctatgacat
    15181 tgaattaatg actatcgaat tgcgtaatct gtatgatagc cgtttgattg atcatgatac
    15241 atacatgaca gcgtggcgca tattaaaaag agagcaccga attgaagaaa gccgaaagaa
    15301 aggaaatttg taatggacaa gaagattatt gggattgatt taggtggaac aacagcaaaa
    15361 tttgcaattt taacaccaga aggagagatt caacaaaaat ggagcatcga taccaatatt
    15421 ttggatgatg gtaagcatat cgttcctgaa atcattgaat caatcaacca tcgtttgaat
    15481 ttatataata tgaaagccga agactttatc ggaatcggca tgggtacccc aggaagcgtg
    15541 gacagcgaag ctggaacggt tattggtgcg tataacttaa actggacaga agttcaattt
    15601 gtgaaaaaac tgatcgaggc aggaacagga atcaaattcg ctatcgataa tgatgcgaat
    15661 gttgctgctt taggtgaacg atggaaaggt gctggagaaa acgatccaga cgttgtattc
    15721 gttactctag gaacaggtgt tggcggtggt atcgttgctg gcggaaattt gattcacggc
    15781 gtgagcggag caggcggaga gatcggccat atcactgtgg atcctgaagg gttcgaatgt
    15841 acttgcggta aacgcggatg tttagaaact gtgtctagcg ctactggtgt ggtacgtcta
    15901 ggtcgttact tggcagaaga atacgcagga gattcaaaac tgaaagcaat gttagacaac
    15961 ggtgaagaag taacaagtaa agatatcttt gaaatggctc aagaagacga tccgtttgca
    16021 ttgatggttg ttgatcgcgt atgcttctac ttaggtttag cttgcgccaa cttggggaat
    16081 acattgaatc cgtcaagtat tgtgttaggt ggaggagtat cggcagcagg agaattttta
    16141 cgtagccgtg tagagaaata ttttaaagaa tttactttcc cgcaagtaag agaatcaaca
    16201 aaaatcaaat tagcagagct tggtaatgaa gctggtgtca ttggtgctgc ctcattagcc
    16261 ttgaaatttg cttaaaaaaa cggaggcatt acatggtttt atgggtaatt aatatcattt
    16321 tgctattgat tgtgcttgca atcatctttt gggaagtcta tctacgagta atggccaagc
    16381 gttcagcaac gactttgaca gaagaagaat ttcgagaagg aatgagaaaa gcacaagtaa
    16441 tcgatgttcg cgaaaaagat gtgtttgatg ctgggcatat tttaggtgca agaaacatcc
    16501 cttacactgt tttgaaagat tcattaggat ctattcgaaa agaccagcct gtctatattt
    16561 acgatcaaaa gaaaagcttg agcatccgta cagcaaataa attacgtaaa gcaggttatc
    16621 aagagattta tttacttaag ggtggatatg acggatggtc tggaaaaatc aaaagtaaaa
    16681 aagtataaaa cagatgattg tatgacagct ttaaaaaccg aatcaatcat tagattttga
    16741 ccaagatcac atgaacggcg caatggatag ttgtgccgtt catttttttt attttgtgct
    16801 attatctaca tatataagct aagcgaaaag gcagaaagaa ggaaacaact atgatcaaat
    16861 taaaacgtgt ttaccttgat gctgaaaaag aggatggcta tagaatactc gtggataggt
    16921 tctggccacg aggaatttca aaagaaaaag cgcagttaga tctgtggttg aaagaaattg
    16981 cacctagtaa tgaagcacgc aaaacttttg accatgtacc agaacgattt gcttcatttg
    17041 caaagaaata ccagcaagaa ttgattttcg aagaagaaaa gaagcaagct atttttaaac
    17101 tcagtcaact tatgaaagaa catccagtca ttacttttgt ttatggtgca aaaaatgaac
    17161 atcagaacca tgctgtttta ttgaaaaaat ttatcgaaat ggaggcatgc aaatgaaaat
    17221 agggatcatt ggtgccggac ctagaggatt aagtatagca gaaaggattc tccataataa
    17281 tcaggacaga cattctcttg aactttttct tttcgatccc aatggtcctg gaggtcgagt
    17341 ttggcggttg aatcagccac cggagttatt aatgaattct gtttctcaac aagtgacgct
    17401 ttttacagat gaaacattgt ccagtggagg attggtaaca cagggaccca acttgtttca
    17461 atggagtaaa ggacctgcta cagattatat cgaacagacg gtaaccaaaa ataagcaact
    17521 aattatagaa gaaatcaatc gtctggaaaa agatcagcaa tctactcgat gtttatacgg
    17581 actctatcaa cgctggtttt tctcaaagtt gcaagaagaa tttccagctt ctattcagtt
    17641 tgtttattct ttagtaaaaa aagtcagcaa aagaaaagat ggttatcttt tacagacaga
    17701 tcagcaaagc tattctatcg atcagcttgt tttagcaagc ggtcactggg aaaatgatga
    17761 gacagaagaa gaaagagtgt gggtagacta cgcaaagaaa aatgatttac tgtatcagct
    17821 tccttctaat cctgcagatg tcgatctttc gaagataccg cctaaagaaa atgtcttttt
    17881 aagaggtcta ggcttaagtt tttttgatta tgtcggattg tttacagtat caagagaagg
    17941 gcgcttcgag aacgaaaaag gaaaacttgt ctatcatcca tcaggcaagg aaccgattgt
    18001 atacagtgga tcaagaaggg ggctccctta ttaccctcga ggaagaaacc aaaagcaagg
    18061 cggatcaatg gcatggccac ggttactaac aaaagaaaac ctggagcaat ggcatcagtc
    18121 gggatttttg actggcgaaa cattttttga ctatctgaaa aaagacgttg agttgtttta
    18181 ttacaagaaa atcatcgaag aatatcattt gccgattcca aagaaaaatt ttgagcatga
    18241 ttttctctcc ctctcttcag aggagtgtct cggaaagcat ccagcactaa tcccttatag
    18301 atgggattgg tcgtatttag aaattcctct tacctatcag gatgagtcct ttgcagaagc
    18361 tagccgagca tttattgaag ggcagataaa agaagcggaa aaaggcaatt gcacaggagc
    18421 atttgcttca acatttgatg cattaaaaga ctggcgtgat ccaatccggc aggctattga
    18481 atggcacgtt ttttctgcaa aggaatacaa agaaatacta tggggatggt ttactcatct
    18541 aaatagcttt ttaacgatcg gtccgccagt cattcggacg aaagaattag cagcactgat
    18601 agatgcgggt atttttcatt tgttcgaacc gccatttgat attcagcaaa agcacaaccg
    18661 ctatgttatt tctctacaag ataaagaaat cagcagtcgc tatttgattg aagctcgatt
    18721 gccacaaaca agcttttctc gttcaagtaa tcctgtcata aaaaatttga aggaaaacaa
    18781 acttgttcgc cctttttcat tttcagataa tacaggaagt tatcattcag aagctataga
    18841 tgttgagcga ggtacgaatc gcgtgattga tgcttcaggg gaaatccagt ctgactttta
    18901 ttgctatgga atacctgttg aaggtgtgga ttggctaact gcagcagtag ctagacctta
    18961 tacagatcca tggaatctga gacaggcaga taaaatagca caacttatat tgactgacaa
    19021 ataaaaatac gcatgatgac ttatctgttc tttcacaagt cttcatgcgt gtttttaaat
    19081 aattattaac gagaaacgcg tttgcgcatt gcttttttac ggttttcttc gatcattgct
    19141 tctttttcgt caattggatc gatcactgtt tttttcacag aagcgaccaa accagcaacg
    19201 gcagcggctg ttgccagact tcctactaat attccagata caaatttttt c
//
LOCUS       DAXWLF010000039        19036 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXWLF010000039 DAXWLF010000000
VERSION     DAXWLF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.039"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(70..279)
                     /locus_tag="ACGE4U_001072"
     CDS             complement(70..279)
                     /locus_tag="ACGE4U_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393253.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(290..1618)
                     /locus_tag="ACGE4U_001073"
     CDS             complement(290..1618)
                     /locus_tag="ACGE4U_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393254.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            1754..1909
                     /locus_tag="ACGE4U_001074"
     CDS             1754..1909
                     /locus_tag="ACGE4U_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFF8393255.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(1983..2570)
                     /gene="yihA"
                     /locus_tag="ACGE4U_001075"
     CDS             complement(1983..2570)
                     /gene="yihA"
                     /locus_tag="ACGE4U_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFF8393256.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(2653..3903)
                     /gene="clpX"
                     /locus_tag="ACGE4U_001076"
     CDS             complement(2653..3903)
                     /gene="clpX"
                     /locus_tag="ACGE4U_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFF8393257.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(4150..5439)
                     /gene="tig"
                     /locus_tag="ACGE4U_001077"
     CDS             complement(4150..5439)
                     /gene="tig"
                     /locus_tag="ACGE4U_001077"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFF8393258.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(5593..6525)
                     /locus_tag="ACGE4U_001078"
     CDS             complement(5593..6525)
                     /locus_tag="ACGE4U_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393259.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(6682..7341)
                     /locus_tag="ACGE4U_001079"
     CDS             complement(6682..7341)
                     /locus_tag="ACGE4U_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFF8393260.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(7344..8369)
                     /gene="sppA"
                     /locus_tag="ACGE4U_001080"
     CDS             complement(7344..8369)
                     /gene="sppA"
                     /locus_tag="ACGE4U_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFF8393261.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(8382..8672)
                     /locus_tag="ACGE4U_001081"
     CDS             complement(8382..8672)
                     /locus_tag="ACGE4U_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393262.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            9082..10887
                     /gene="glmS"
                     /locus_tag="ACGE4U_001082"
     CDS             9082..10887
                     /gene="glmS"
                     /locus_tag="ACGE4U_001082"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFF8393263.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(10959..12314)
                     /gene="glmM"
                     /locus_tag="ACGE4U_001083"
     CDS             complement(10959..12314)
                     /gene="glmM"
                     /locus_tag="ACGE4U_001083"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFF8393264.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12338..13507)
                     /locus_tag="ACGE4U_001084"
     CDS             complement(12338..13507)
                     /locus_tag="ACGE4U_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFF8393265.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(13504..14391)
                     /gene="cdaA"
                     /locus_tag="ACGE4U_001085"
     CDS             complement(13504..14391)
                     /gene="cdaA"
                     /locus_tag="ACGE4U_001085"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFF8393266.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(14580..18260)
                     /gene="nifJ"
                     /locus_tag="ACGE4U_001086"
     CDS             complement(14580..18260)
                     /gene="nifJ"
                     /locus_tag="ACGE4U_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFF8393267.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            18458..18979
                     /locus_tag="ACGE4U_001087"
     CDS             18458..18979
                     /locus_tag="ACGE4U_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFF8393268.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         5337 a         4099 c         3366 g         6234 t
ORIGIN      
        1 agtcaaaaaa aaataaaaaa ccagtcaaaa agactggttt tttatttttt tgtctatcat
       61 caagccgttt tatcgttttt tattttcgct tcttgttttt ttcatataaa cagaagcagg
      121 ttttctttcc ttatgaagaa gataataata aagtcccatc ccgccaaaca tgaccaaaaa
      181 accgatgact gatcgaatag gaaaattagg aacaaaaaag agacagacgg ataataatga
      241 tgccggaacc attattcgat aatagagttg tttatccatt ttcttcacct catatttttt
      301 gtaaagtaat caattgttct aattctcggt aatctaaaaa ttgcacacgc agatgctgct
      361 cacctttctt gacatcgaat tggatacttc gccgcttttt ctttattcgt ttgaaggtaa
      421 catcagagag tctatgtttg atttccttta acggtaattc tgacagggat tctgaaggaa
      481 catagattct taaggataaa agatactctt gatatttttc atctgttacc tctacttcta
      541 gatattccct aatataataa caataagctt cgattccatt atccaaagca acagtcggat
      601 cgataacttt atagcacatc atgcgcgagc gattctcgtc aacccaagga aaatacgcct
      661 gagcatttag tgttccgcga acacgccttt ctttataatc tccttgttga atatcataac
      721 tttttgttac aaaaaatgaa gcattctttt taggaacagg ttgcatttgt gcattctgat
      781 actggacaga ttggatcgta tggtcagtgt aaatgtattc aacgaatggc ggtttcaata
      841 aaccgacacc attgtcccaa gcaagatgat caaacccaag ctggtcccta aaataggatt
      901 gaaacatttg attgtttggc ttttgccagt taaaggggtc tcgataaagc tggatacata
      961 tgacttcgtt ttctaattcc ttcacttttt ctacttgttg gatgtcgaga aaaatcggtt
     1021 tagggatgat ttgatgataa tagcgactat aatacatttc ccaacacgaa gtgaaacata
     1081 aaccttggta aaaaagatcg tatcctggaa attggttgca atcgactact acttccccgt
     1141 tttgttgttt cattttaggt aaatgttcga tttcatcctt tttctcaaac aatagccgtt
     1201 gagagatctg ttttgtattg tgatagataa actcgctata agctcgcata taggcgaaca
     1261 ccccatgagc aatcattttt ttctgaatga agtctcgaat caaaaattgc ttttctttga
     1321 aaacagagat agacaataca ttttttatca ataattgatt atcttccaag cgaaaactac
     1381 attcgttctc gtcatttcct acaactaacc aactttcgtc tcttaccgat gctagcatta
     1441 ttttttcttg aagctttcga agatcatacg gatccgcttg ttgtgtgaaa gagcggataa
     1501 atgttggctg gatcaacaga cgtgtcaaaa tagcccaatc gcttatttcc tgtttttttt
     1561 cttcttcatt taagtctgtg agctggatag tcatcaataa tttatcttcg ccaatcattt
     1621 tctcacctct ttaaaatctt tttataattt aattataacc aacgataaag aaaaactaaa
     1681 actttatgtc ttagaaacca atttggacga ttacatgata gactaagtaa aaagaaacaa
     1741 agaggtgttt acaatggggt tgaaatttga acgtacagat aatttagata aacttttcga
     1801 atcatttgct gtcgatccgg aaaaaacagt tgcaccaaaa gaagagatcg atcgatttct
     1861 aaaaccacag ccacaaaatg aaaagaaaaa agacaagaaa caaaagtaag ttaagctcaa
     1921 aaaaagactt accagccaaa cttttatgaa agagtttgac tggtaagtct tttttattct
     1981 ttttatgatt caattgcttg ttcgattgct gcccacgctt cttcttttcc ctgttttgta
     2041 acggaagaaa agagaataaa cgtgtcattg cgatcaaaat ccaacgcttt tttgatttcg
     2101 ctctcatgct tgttccattt accacgaggg atcttatctg ccttagtggc aacaacaatt
     2161 acagggatat cataatactt caaaaattga tacatctgga tatcttcttg agaaggctta
     2221 tgacgcaaat ctactaaaga aacgaccgca cgtagctggt ctctagtcgt taaatatgtc
     2281 tcgatcatct ttccccattt tgctcgctca gttttggata cttttgcata cccatatccc
     2341 ggaacatcga caaaatgtaa tgcgttctcg atcaaataaa aattcaatgt ctgggtcttt
     2401 cctggcttac cggaagtacg ggctaaattc tttcgatcaa tcaatgtgtt gataaaagaa
     2461 gatttcccta catttgatcg tcctgccaga gctatttctg gcaattgtgt atctggatat
     2521 tgttttggtg ccactgcact tatgataatt tcagcttgat ggactttcat ggtctctctc
     2581 cttaacaagg tgatgaataa agcaagcggc tgtcgcaaaa gccaaaatga cttttacggc
     2641 agcctctttt catcaacccg cttttttctc tgaatcttta tagattactg ttggctcacc
     2701 agacaattca gctgcttctt ttgtgataat cactttttcg atgttttcat ttgaagggat
     2761 atcaaacatc acatccatca tgatttcttc aatgatggaa cgaagtcctc gagcaccggt
     2821 attgcgttcg attgcttttt tcgcaatggc tctcaacgct tcaggttcga attcaagttc
     2881 ggtattatct agagaaagaa gtttttgata ttgtttgact agcgcatttt tcggctcagt
     2941 caagatccgt accagatcat ctgttgtcaa tttctctaaa gctgccataa caggtaatcg
     3001 tccgataaac tcagggatca atccgaattt tagtaaatct tcaggaatga tatgctgcat
     3061 gacactttcg tcttcagaaa gtttttggtt ttgtgtacca aatccaatcg tcttttcgcc
     3121 catacgattc ttcacaatcg tttcgatgcc atcaaatgca ccacctacga tgaacaatat
     3181 gttcgtcgta tcaatctgaa taaattcttg atgtggatgt ttacgtcctc cctgaggtgg
     3241 cacgcttgca acagttcctt ctagaatttt cagcaaggct tgttggacac cttcgccgga
     3301 aacgtcacga gtaatagata cattttcgct tttacgcgca attttatcga tctcatcaat
     3361 atagatgatc cctttttctg cacgttccac attgtaatca gcagattgaa gcaatttaag
     3421 caaaatgttt tctacatctt cccccacata accagcttct gtcaaacttg tcgcatctgc
     3481 gattgcaaaa ggtacattca atgtttttgc taatgtttgt gctaaaaatg ttttaccaga
     3541 ccctgtaggt ccgatcaagc agatattgct tttttgcagt tcaacatcat ctgtgttttc
     3601 ttgtgcttgc tgattgacac gtttatagtg attgtatacg gcaacagcta acgagcgttt
     3661 tgcgcgatct tgtccaatca cgtagttatt caatacttca agaatttcta gtggtttagg
     3721 gacttcagtg aattcgcgta cagcttcttc ataaaattct tcatcaatga tttctttaca
     3781 taggtcgata cattcattac agatgtagac ccctggacca gctacgatct ttttcacttc
     3841 ttcttgggtt ttcccgcaaa aagaacagcg tacggtctca ttgctacttg gattgtcata
     3901 catggtcttc accccttaac ttgcaaaaag tatgcaagaa actatcaaag actttacagt
     3961 cttttactat cataacatag tttagtaaaa agaaaaagtg tttaacaaag gatcaactga
     4021 cctatacctt tgcatttttt actcttaaca aaaaataagg aactgtgaca aaatcgtgtc
     4081 acagtccctt atttcgaata aacaattttc tagaagtagc ttcttcagaa atgatttatt
     4141 acttgatttc tattctttcg cttctgtcgc tgtacctgtg atcaattcga ttgctttttt
     4201 cattgtcaca tcatgttcca gcatatcttc tgtcaacacg cgtttgattt gttcgattgg
     4261 catgttgtat gtttctgata attcttggat ttctgcattg atttcttctt ctgttgcttg
     4321 aagattttca gcagctgcaa ttgcttcgat cacaagattt gttcttgtgc gcatttctgc
     4381 ttcaccttcg aattgtttat gcaagtcttc ttctgtagaa ccagttaatt gatagtacat
     4441 ttcaggagag ataccttgac gttgcatgtt gttcaagaat tcgtccattg aacgatgtac
     4501 ttcatcatga accatcacgt gtggcaattc aacgatttct gcgttttcaa cagccatacg
     4561 gatcgctgct tcatcttttg catcgtccgc tgctttttct tttgtttcta ctaattcttt
     4621 gcgatatttt tctttcaatt cgtctaatgt tgatacttcg tcatcaacat cttttgcgaa
     4681 ttcgtcatct aattcaggca cttcttttgc ttttacttca tggattgtca ctttgaagac
     4741 tgcttctttt ccagcaagat cttctgcttg ataatcttct gggaaagtta catttacatc
     4801 tagactttca cctgatttat gaccaactaa ttgatcttca aaacctggaa tgaatgagcc
     4861 tgaacctaat tcaagtgagt aattttcgcc ttttccgcca tcaaacgctt cgccatctac
     4921 aaagccttcg aagtcgatta ctactgtatc gccattttca gctgcagcat cttctttgat
     4981 aactaattca gcttgtgctt cttgttcacg tttgatacgt gcatccacgt cagcatctgt
     5041 tacttcacgg tcttgttttt caacaactag atctttatat tgtcctaatt ttacttctgg
     5101 tttcactgtt acttcagcag tgattaccca gtcttgacct ttttccatgc tttcaacatc
     5161 gatttttggt tgagaaacag gatcgatgcc agcttctttg acagcagctt cataagcttc
     5221 tggtaataca gcattcaatg catcttcata taaagcttct tcaccgtaca tacggttgaa
     5281 tacttgacga gaaactttcc ctttacggaa tccaggtacg ttcaagcttt ttttcacttt
     5341 attaaacgca gttgttaatc ctttttggat ttcgtcttgc gcaatcgtaa attttaaaac
     5401 accatcattc gtgccttttt tttcaaatgt tgcagtcatt taattccctc cgagtttaga
     5461 aattcatttt atacatcaaa aacgtttcat gcataccttt aaatagtaca ctaacccttt
     5521 caaattgtaa acatattgaa tggatagaat ccttgttttt cataacattt ttatcttttt
     5581 cactgataaa atctaatgga agaagatcat ttgctgcaaa attttttctc ttttttttaa
     5641 aaaatcttgt ttttcatctt ctgtaccttc atacatcgtt tttcctgtac acgcctctac
     5701 agtcagttga aaccagcgct tcgggtcttc gatgtatgta tcatagacag gataaacaat
     5761 tgcagattgc aacgtgaatt cttcatttaa cacggaacta agcagcgcat cttcattacc
     5821 gtaacgatct gaaaatacac ataattgctg gtaacttgcc tgctgctgcg gctttggcag
     5881 atcttttggt atgactttga ctaacttttc ttcaatcgtc aaatagaaaa caggatggca
     5941 ttcgttcaat tgcctaagtg tctccagtaa ttttgccctt gctaaaatat ggacctctgg
     6001 cgatacaata agtttactcg ctgtttggat aaactcatga taaggaagct gttcgatctc
     6061 atcaatagac gcaagctgac gtgcaggttc caacgaagga agttcctccg atttattgtg
     6121 gagtgccttg atcttctgcc tctccatttg tccggataat tcttctgcaa attggatttt
     6181 ttcaatgacc cgctgctcga aagcttcata aaaatctgta cttgctatca attttctagc
     6241 atataaaaaa tcttgtgtac tgattaacaa gtcaaaataa aattcagcaa actcctcatt
     6301 ttgaagatac tgtttttcat gcaagagtgc ttgcttcaat gcttcttttt tctcgtccaa
     6361 ctcgtaaaga caaaaaacaa gcaggctatt agcctcaaag ctttctttca actcgtaagc
     6421 tcgttgaaat aattcttttg cctgctgata ttgctggttt gctagtgctt gttgtgctac
     6481 tcgcattaaa ttctcatatt tttctggaaa atcaatcggc ttcacgcagt gtcctcctct
     6541 tgctcatgtt cagctcatgt tcaatagtag caaaacaaaa aaagcatgac aattaaaaat
     6601 aactgaagca ccctctaaaa gctagatttt agatctgact tttagaaggt gctttcaact
     6661 gttactgctt tttattgctc atcagctgtt gacttcctgg ttgaatgctt tgatcagatt
     6721 gatcgtaaca acacttgtat tagtgaaaaa atcgcctaga tgttgctttt tctgattaaa
     6781 gattgctgga agatacccta acatcagcaa aggaaatttt agaatgaacc ggcaagcacc
     6841 ttctctaact aaaacggttg accagctaag ttcttcttca tcaaagctga tgactcgaat
     6901 accaaagatc attttcccaa tcgtttgacc atgattgaat ttcgtcaata aaataaaata
     6961 cgctagataa ataagtaagg ctgtgaatcc gtagatactc agataagaat ctgaaaaatt
     7021 aagccaacct agatttttgg caataccaag tgttaaacgc gtaatagctc cgatacacaa
     7081 aagatcgacc aagaaagcaa acattcggat aaaaaaacct gcatagaaat acttaggaaa
     7141 atcatttgct tttggcggtt catttcttgt gtccgtatac tttttccatt tacgttgttt
     7201 ctttttgatt tcttcgggcg tcaaaggctc tctttcaaaa gagctcagta ccttttggga
     7261 aaagttggag tcaggtccta cagcaggctg ctgttgctta tctttttctt gttccttatt
     7321 ttcatttgaa agctctgaca tgcttactca cctccgtaat aatacattgc ttttggtgat
     7381 tccgctgtcc ctagattctc aagaacggat aagatctgac tagtctcaga aggcttcaat
     7441 ccctggaatt ctgctagttt acttcctaac catgtactag caaacccaga tggcgtggaa
     7501 gaatattcta ctagttccgc atcttcaagc tgttgttcct ttcgcatagc ttttaaagca
     7561 tcttcaggaa atcccaattc atcgactaaa cctacttctt ttgcttggac accatcataa
     7621 atccttccat cagcaatttt tttgacttct tcttcagatt tattccgtcc ctcgctgacg
     7681 attgctacaa aacgactata tgcgctgtcg atatatgctt gaagcaccgc ttcttcttct
     7741 tttgtctctg gtcgcgttcc tgaacccata tctttcaacg ctccgctttt aactgttgta
     7801 tcttccacac ccagtttttc tagaagtcct gaataattca atcctgacat gatcacacca
     7861 attgagccgg tcactgtttc ttctgttgca aagattttat ctgcttgagc agaaatatag
     7921 tagccaccgc tagctgccat gtttttcatg ctgacataca ttgggatatc atgttcttta
     7981 cgaatcgtat ccaatttctt agcgatttcc gcactttcgt agacaccgcc accaggagaa
     8041 tttacttcta aaaagatacc tttgactgtt ggatcttctt caatcgcctt cagttgttcc
     8101 ataaaatttt tatgattgta accttcagaa gcaaataaac ctgaagaccc tcccgcagca
     8161 atcgttccat ctactgataa ttttacgatt ttttgcgtac tgtctccctc ttccaacaca
     8221 tttgaagcta gttcttcatc cccatataac caactgttca atccctcaat ttgatcgttt
     8281 tcctgaggct tagtcaaact agctgaaact aacgaaacca caaataatcc tgcagcaatc
     8341 agaactgctg cccaacgtct cttattcatt ctttctcctt tctaatacaa gttttttatg
     8401 acgatcacag tttctgactg ctcagaagtg tcgaaccgct ctgctttttc ttcccgcgca
     8461 tacccggaaa acgactcttt tgtcaattca tcttctaccg taaactcacg agcttcagca
     8521 acttccaagg catcaggtgc gatttcttgg atgctatccc agccagtatc taaaaatatt
     8581 ttcgtatcat ctgatacacc agctgatgct tcgaatttgg acaattgttt ttttaacatt
     8641 cccagtgtca tgatctcaat ttttgaatcc atttttactc ctcctctatc cgattacttt
     8701 ctattgtacc gaactcctga aaaagatgat agataaaaac ccattatttt ttgacataaa
     8761 aagagcattt gacaaagcat ttgatatata ccaatttatt ttttattctt tttttgttga
     8821 caagaacatt cgcaaaaggt tatgatttta aatgtttcca gataaaaaac aaaagaggag
     8881 gatagttata ccttttagga tagcgccagg tctgtgtaaa cagatgacga ggagagggtt
     8941 tatcgaaaga ttcggcggat ggccctaggg actgcactct acagaaaatc cacaaaaaat
     9001 atctgcaaag ataaaacaaa aggattttcc tgcagctgga agcattcata cgaattcatt
     9061 atagaaagta ggatgttttt tatgtgcgga attgttggaa tgattggatt aaaaaatgta
     9121 acaccaggat tgattgacgg tctagaaaaa ttagaatacc gtggttatga ttcagctggt
     9181 atctttgtct cagatggcac aactgattat ctggtcaaag cacaaggcag aattcaaaat
     9241 ttgaaaaaca aaataaatat tgatactaca ggaaatatag gtattggtca tacacgctgg
     9301 gcaactcacg ggcaaccttc tgaagaaaac gcccatccac atacttccca aagtggacgc
     9361 ttcgtgctcg ttcacaatgg cgtaatcgaa aactttgaag aacttaaaat tgcttattta
     9421 gcaaatgacc actttattgg tgagaccgat actgagatta tcgctcatct aattgaaact
     9481 ttcgcgaagg atactacaac gcaagaggcg tttttaaaag ctttgcgggt catcaaaggt
     9541 tcttacgctt tcgctttgat tgatcggaca gcacctgatg ttatttacgt cgctaaaaat
     9601 aaaagtccac tattaatcgg actcggcgat ggattcaatg tgattgccag cgatgcaatg
     9661 gctatgcttg ctcatacgaa ggaattcgtt gagattgaag atgaagagat ggttaccgtc
     9721 acatcagaaa aagttattat tcaaaatttt gcaggtgaca tcgtagaacg ttcttcattc
     9781 gaagcacaag tagattcttc agatatcgag aaaggtacct atccttttta tatgctgaaa
     9841 gaaatcgacg aacagcctat tatcatgcgt cgtatcgctc aaaaatacgt aacagaagac
     9901 aatcgtgtct ctcttgatca aaagctggtc gatacattgt ctgatagcga tcgaatctat
     9961 atcgttgctt gcggaactag ttaccatgcg gggttagcag gtaaacagac tctggaaaaa
    10021 ttgacacaga tccctgttga ggttcattta gccagtgaat ttggctataa cacacctctt
    10081 ctttcaaaaa aaccattctt tattttcttg agtcaaagtg gcgaaacagc agatagccgc
    10141 caagttctgg taaaaatcaa ccgtctcggc tacccttcac ttacgattac aaatgttgct
    10201 ggttctactc tttcgcggga agcatccttt acactgctat tgcatgctgg acccgaaatc
    10261 gcagtagcat caacgaaagc ttatactgca caaatagctg tactaacttt attagccaaa
    10321 gctatcggtg ataaaaaaga atttgaaact tctcttgtat tcgatatcac tcatgaattg
    10381 agtttagttg ctaatgcgat ggaatcggtc atcgcccaaa aagattattt agaagagttg
    10441 gcagaagtat atctcagcga tacaagaaat gccttttaca ttggacgtgg cgctgactac
    10501 aacgtttctc ttgaagctgc tttgaaattg aaagaaatca gttatatcca ggcagaaggt
    10561 tttgccgctg gcgaattgaa acatggaacg attgctttga tcgaagaagg aacaccagtg
    10621 attggaatca tcagtgaaga agtgactggt gcgcatactc gaggaaacct gaaagaagtc
    10681 gaaagccgtg gcgctaaaac actagttatt gtctcagagg gattagaaaa ggaaagtgac
    10741 cagctggtgc taccaactgt ccacccttat ctaactactc taaccacagt cgtaccgact
    10801 caattacttg catattacgc aacgctatta cggggattcg acgttgacaa accacgtaat
    10861 ttagcgaaat cagtgacagt agaataataa aagaacaagg accagtgtca gacatctgac
    10921 actggtcctt gttcttttat tttttatgaa aatatctctt aatccagtcc gatttcttct
    10981 ttgactactg ccgcgatttt atcgacatag taattaactt tctcatcagt aggcgcttct
    11041 gccatcactc gaagtaatgg ttctgtaccg gaaggacgaa ctaggattct tccttctgat
    11101 cccatttcag cttctgcctc ttcaatcact tttttgattg ccggaacatc catcgcaccg
    11161 tttttatcac ttactcggat attcactaat ttttgcggat agattgttac ctctgctgca
    11221 agctctgata atttcttacc tgtctgtttc atgatattca gtaactgaat tcctgaaagc
    11281 atcccatctc cagttgtgtt aaaatctaag aaaatcatgt gcccagactg ctcgccgcca
    11341 aagttataat cgtttttgcg catttcttct acaacgtaac gatcaccaac ttgtgtaacg
    11401 acatctttca aaccgattcc ttccaccgct ttgtggaatc ctaagttact catgacagtc
    11461 gtaacgatcg tatctttttt caatcgattt ttttctgcca agtatttagc acagataaac
    11521 atgattttat caccgtcgac aatgtttcca agttcatcca ccgcaataat acgatcgcca
    11581 tcgccatcga aagctagtcc agcatctgct cctttttcaa cgaccatctc agctaaacgt
    11641 tctggatggg tagagcctac gccatcattg atatttaacc cgtctggatt tgtgcccatg
    11701 gtataaaaat ctgtttcaag atcggcaaac aaacgattaa cacttgttga agttgctccg
    11761 tttgctgcat cgacacaaat ggtcaaaccg gaaaggtcac cgttgatcgt ttgtacaagg
    11821 aattgagaat atttcaataa gccttctgga aattcttcta cagttcccaa tccttcagct
    11881 gatggacgag gcaattcatc cacttcagca tctaataatg cttcaatttc tagctcttgg
    11941 tcatctacta gtttaaaacc atctgcaccg aaaaatttga ttccattatc ttgtgctgga
    12001 ttatgagaag cagaaatcat gacccctgcg cttgcttttt gcaaacgtgt cagataagca
    12061 acacctggtg tagagatcac acctagctgg aacacttcga tacccaccga taaaagtcct
    12121 gaaatcagcg cttcttctaa catttgacct gagattcttg tatcccgccc tactaacacg
    12181 cgtgggcgtt ttccttcttc ttcatgctgg cttaggacat aacctccgca ccgtcccagc
    12241 ttaaatgcaa gttctggcgt aagttccttg ttggcttcac cgcgtacgcc gtctgttcca
    12301 aaatatttac ccatagttgt aaaattactc ctttaactta attttctgag gaatttgttc
    12361 tgctcatttg tgttgattcg cttgtttctg atgtttcttc tgtacttgtg gtcgagctgc
    12421 ttgtcccaga ttccgaactg gaagaaatgg ttgttgtcga tggttccgtt cctgaatcag
    12481 cagatgaaat acttctactc gtacttgtat tttcttgtgt ccgactgctc gtcgaagcag
    12541 agctactgct tgacgtttgc aacacgggtg taatctccac actaactgat tcgggcttga
    12601 tacttaaccc ctcttctact ggtatttcaa ttttacgagt tgttcgatga ttgatacctg
    12661 agacattgat cggtacagca atactgtcac cgatcgatgc aagcaaatcg ctagagccgc
    12721 ttacttctgc ttccagtttt gaaaaactga aatcatagct ttctaccccg gcagctggtg
    12781 ttccttgctg tatcccataa agtcgtaccg acttgcttgg cttgatcgaa tcagctctca
    12841 ctgttacttg cactggatca gagacgatac ttaatacttc ccctgctgca ttcagcgcct
    12901 gtattgggac ctcactatta atcccatcat ctgtgaccat cgaagcgtcc acatcagcta
    12961 ctactcgttt gatttctgcc aatgttttgt cacctgtagt gatagagact tttttaggac
    13021 taacggaagt atcatcaagt tcatatccag atggtgtgaa cgttgaagga atcaacgttt
    13081 ccacatcgaa atcttttatt acctttttct caaccgttac tgtaatagtc gatggttcga
    13141 tctttgctgt gacagcactg ctcaaatttt gaatttttag cggaacctca tgtgttcctt
    13201 cgcccagctt agtgatatct gctgtgacgc ggaacatccg tgtatcttcg tttgcctctg
    13261 cattcaactg aactcggttt gcactggaaa gtttgacagt cactgcattt tcatatccat
    13321 ggatatagta gcgctttgaa tcataagaaa tcttgattgg caccccagta actgtttgat
    13381 tatacgcttc atttccagaa agcgtatttg gaacggactg gccatttgca ttgaagtaca
    13441 gcgcaatact gaacagcaat gcaataaaac tgtaaagaat gcttgtccgt ttctcttttg
    13501 agatcatttg cgatcccctc ccttagtcat accgtctagg aaagattgaa tcacgttttt
    13561 cttgtttttt gttttctctt cagcaatcag ttctctacgc aaaattgcca aatattcttg
    13621 ctgggataag ccagccatca gttcattatc taatgtaatg ctgacatccc cagtttcttc
    13681 agagacaacg atggtaatcg cgtcgctgac ttcgcttata ccgacagctg cccggtgtcg
    13741 agtcccgaat tcttttggaa tcaacatact ttctgataga ggaagatagg cgcttgctac
    13801 tgcgatcttt ccttctttta caataactgc accgtcatgc agcggtgtat taggaataaa
    13861 gatattgatc agcagctctc cagtgatatc tgcatctaat gcaattcctg tctcaatata
    13921 ctcatctaat ccagtatgcc gttcgatagt aattagcgca ccgatttttc gcttggacat
    13981 atattggatg gctttgtcaa aagacaagat catcttctca tcttcctgct gttcgctttt
    14041 tgattgtttg aaaagcgaac tgcgtccaag atgttccagt cctctccgga tttccggctg
    14101 aaaaatcacg atagcagcta tcaccccgta cgtaatgact tggttcatca gccaagacaa
    14161 ggtgtgcaga ccaatgattt ccgctacgat tcggataaca ataaaaatag ctacaccttt
    14221 cagaagctga atcgcttttg tgcctcgcag cagttggatc agtttgtata caagatacca
    14281 gacaaccaga atatcaagta tgttcaccaa aaaattcaga gagaacaagt caccggaaaa
    14341 catttgctgc caatagtcca aattaaaaag ttccccgatt tgaaatgaca taaaaagcct
    14401 ctcttctctt ttgagatttc ctctctagta tatcatatgt tttaatttta gaggctgatc
    14461 tgctcgtatt tttcttgaga aaaacgcata attttcagga aaattgcaag tatgcgtttt
    14521 atttttctct gcaataaata tttttaaact tcagagaatt attcattata ttttttctgt
    14581 tatttttcct ttttttcttt tgtagcttgt tccatttttt tacgtcgttc tgctctggca
    14641 gctcttcttt cttctgcttc aaggtccgtc gttttttctt ttctagtctt aacgggtaca
    14701 tcgatagatt cctctggttc aagtcgtttc ttgatttttt ctaattctcc cgtcagtgca
    14761 gcatagttgt aaaaacgtgt tttagcgtct tggatagtct tttcaaataa agcttttgca
    14821 agatttggat ttgcgttctc tagcgaggaa aagcgaactt gtttcctcat gaaatcaggc
    14881 atagcagaga aatcaggttt tttgtagtcc aaagtcattg ggttcttgtt tttttcgacc
    14941 attgcagggt tatatcgata taatgaccaa tagccagaag aaacagcttc ttttgcttct
    15001 ttaagcgatt ctttcattcc accaagcaaa ccatgtgtga tacaaggcgt ataggcaatg
    15061 attaaagaag gtccaggata cttttctgct tcttcaaaag cctttatcgt ctgcatttga
    15121 ttggcacctg aagcaatttg cgccacataa acatttccat atgtcatcgc catcattcct
    15181 agatctttct ttgaagcata ctttccacta gccgaaaatt tagcgattgc cgaagctggt
    15241 gttgcttttg aggtttgacc gccagtattg gaatagactt cgttatccaa aacaagaata
    15301 ttcacgtctg ctccgctggc aagtacatga tcgatcccgc caaaaccaat gtcgtaagcc
    15361 catccgtctc caccaatcat ccattggctg ttttttacga atagatcctt tctcttgtat
    15421 atatcctcca gttcagggac aattgatcgt tcgctttcga tcgcagcttg cagttttgct
    15481 gctctttgct gcgttccttc gctctcaaac atgtgtacca accagtcttc catcaattct
    15541 cgtagttctt ctgatacttt tggcaatact tctttgattg aacttgccaa agcctcgcga
    15601 cgagtctgtg ccgctagaag cataccataa ccaaattcag cattatcttc caataaagaa
    15661 tttgaccatg caggcccttg accacattca ttggtcgtat aaggagaagc aggagaagca
    15721 ccgccccaaa tagaagaaca gcctgtcgca ttcgcgatca tcattcgatc accgaacatt
    15781 tgtgtcaata attttacata aggtgtttcg ccacagcctg agcaagcgcc cgagaattca
    15841 agaagcggtt gattgaattg tgtagacaag acagtattct ttcctttgac tggatttgct
    15901 ttttgtctta atgtcattgc aaaagcccaa ttcacagctt gttctttctg ttcttcataa
    15961 ggcttcatca ctaaagcttt ttcttttgcg ggacaagcct ctacacacag accacaacct
    16021 gtacagtctt ccactgaaac ttggatacgg tattttacgc cgtctgcgcc tcgcatttct
    16081 ctcacgataa atccttctgg cgcttcttcc atttcctctt catctgctaa aaatggacgg
    16141 atggcagcat gaggacaaac aaaggcacat tcattacaca tcgtacagcg atcactaatc
    16201 cactcaggta cttctaaagc gacgccccgc ttttccatag aagccgttcc taaaggcatt
    16261 tcgccagttg tcattccatt tgctacaagt gtaccaacag agagttgatt tccttcctgt
    16321 gcattcactg gttcaagtat ttcttgtata taaccagtgt tccttttatt gattttttca
    16381 ggcatctcca acgttctcca atggtctggg atcgcgactt gatgaagcag ttcggtcgtc
    16441 cgatcgattg cttgtatgtt tttttctacg atagccattg atttatgtcc atagcttttc
    16501 agggcttctt cttttagcag cggaagcacc tcatcaaaag gaatgatgtc agctaattta
    16561 aaaaaagccg tttccattgc cgtattgatc cggcgtccca aaccgacttc ttgggccaat
    16621 ctcatggcat tgatcgtata gaatctaatg ttattttcag ccaagtaacg ttttaatttt
    16681 aagggcagat gcgtttctaa ttgttcgtcg ctccaaagag tattcaataa aaaaatgcca
    16741 cctggcttca atcctttgac caagtcatag ctatgcaggt aagctggtgt gtgacaggca
    16801 acaaggtccg catgctcgac aagatacgct gaacgtatcg gcgtatctcc aaaacgaaga
    16861 tgagagacag tcagaccgcc agattttttc gaatcataat aaaaatagcc ttgcgcatat
    16921 ttatcggtgt gatctccaat aatcttgata gctgatttat ttgctccaac tgttccgtca
    16981 gatccaaatc cccaaaactt ggcctggaat gtttgcggtt ctgtcaaatc cagactttcc
    17041 tttggttcca aagattgata agtcacatca tccacgatcc ctatggtaaa ccgttttttg
    17101 cgtatgcttg ggtctttttt caattcatca aaaactgctg agatttggtc gggagtcaca
    17161 tctttagacc ccaatccgta gcgaccacca ataacagccg ggcgaagttc agaatcatat
    17221 aacgcacttt gaacgtctaa taagagaggt tctccacctg cccctggttc tttacttcga
    17281 tcaagaacag caattgtttt tactgtatga ggcaactttt gcacgaatgt ttccactgga
    17341 aaaggacgat aaagatggat attcaaaaaa cctgttttcc gtccgtttgc attcaagtaa
    17401 tcaatcgttt gttcaatcgt ctgtgcaact gatcccatag aaacaatgac ttcttccgca
    17461 tcgtctgctc cgtaataagt caccaagtca taagaagttc ctcgtaatcg gttgatctct
    17521 ttcatatatt tttgaacgat ttcaggtaat ttatcataat aggggttgat cgtttcccgt
    17581 tgttgaaaat ggatgtctgg attctggttc gtaccactaa cagtcggatg attgggattc
    17641 atccctcgtt tacgaaatga agctaatttc tcctgattga tcagaggagc taattcttca
    17701 tattccaaaa cttcgatttt ttgaatctcg tgactcgtac ggaatccatc aaagaaattc
    17761 aaaaaaggta cttctccttc aatagaagca agatgggcaa caggtgctaa atccatcact
    17821 tcttgtacac tgctttcagc taacatagca aatcctgttt gtcttgcagc catcacgtct
    17881 ccgtgatctc cgaaaatatt caacgcattt gtcgtcactg cccgactagc aacatggaaa
    17941 acacttggca gtagttcccc agcaatcttg tacatattcg gtatcatcaa aagcagccct
    18001 tgagaagcgg tatatgtagt tgtcagtgca ccagttttca atgatccatg gaccacacca
    18061 gctgctcctg cttcggattg catttctact acttttactg gttgaccgaa tacattttct
    18121 tttcctgctg ctgaccattg atctactaat tcagccatgg tcgaactggg tgttattgga
    18181 tatattgcag caatctcggt aaatgcataa gaaatatatg cggctgccgt attgccatcc
    18241 attgttttca tttttcgcat gattcatcat ccttgttcat attctctctt attatctgtt
    18301 ccattataac gtaaaacatt ccattttttc ttttatttat aaaaatattt cacatatgtc
    18361 tatttgaatt ggtcatctaa tttttagaca aacagtctaa atcgttgcct ttttttgtca
    18421 gacatgtata aactataagc gaaagaggtg ttttaacatg gatatatcag tgattgatgc
    18481 aacgaaaaca aatacacaaa aaggaatcct ttatggttca tcgaatgctc ctaaaaaaat
    18541 ggtcgaattc atcaacctat cttgtccgta ttgccgacaa tggtttgaag aatcttatga
    18601 gctattagaa gaagcagtcc aaagcgggca attgcagcga gtgatcaaat tgttcgataa
    18661 agaaaaagaa agcttgcagc gaggaaacgt gatgcaccgg tatctcacaa tcagcgatgg
    18721 tcaaaaagcg atcaaagaga tcaaacaaat attcgatact caagacgagt ggaaacatct
    18781 ttctctccaa gaagtagcgg attttgcagt agataaacta aagctgacag aacaaaaaga
    18841 cgaacagctg agccaagctg tcatcaacga agctgagcaa gcacatattc gatttgttcc
    18901 gactgtcatc ttgggtaaag aaatctttga tgaatctatt tcaatagagg aattaaaaga
    18961 attgatccaa acaaaataaa aaaataagga gcgtaggaca aaagtaaaga acacttttgt
    19021 ctcacgctct aaacac
//
LOCUS       DAXWLF010000040        18210 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXWLF010000040 DAXWLF010000000
VERSION     DAXWLF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.040"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            34..1695
                     /locus_tag="ACGE4U_001088"
     CDS             34..1695
                     /locus_tag="ACGE4U_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8393269.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1765..2112)
                     /locus_tag="ACGE4U_001089"
     CDS             complement(1765..2112)
                     /locus_tag="ACGE4U_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFF8393270.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2196..3137
                     /gene="opp3b"
                     /locus_tag="ACGE4U_001090"
     CDS             2196..3137
                     /gene="opp3b"
                     /locus_tag="ACGE4U_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFF8393271.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3138..4181
                     /locus_tag="ACGE4U_001091"
     CDS             3138..4181
                     /locus_tag="ACGE4U_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8393272.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4187..5257
                     /locus_tag="ACGE4U_001092"
     CDS             4187..5257
                     /locus_tag="ACGE4U_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393273.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5247..6200
                     /locus_tag="ACGE4U_001093"
     CDS             5247..6200
                     /locus_tag="ACGE4U_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393274.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6212..7222
                     /locus_tag="ACGE4U_001094"
     CDS             6212..7222
                     /locus_tag="ACGE4U_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFF8393275.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7507..8328)
                     /locus_tag="ACGE4U_001095"
     CDS             complement(7507..8328)
                     /locus_tag="ACGE4U_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8393276.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8425..8703)
                     /locus_tag="ACGE4U_001096"
     CDS             complement(8425..8703)
                     /locus_tag="ACGE4U_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393277.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8971..10980
                     /gene="metG"
                     /locus_tag="ACGE4U_001097"
     CDS             8971..10980
                     /gene="metG"
                     /locus_tag="ACGE4U_001097"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFF8393278.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11459..12046)
                     /locus_tag="ACGE4U_001098"
     CDS             complement(11459..12046)
                     /locus_tag="ACGE4U_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393279.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12221..13543
                     /locus_tag="ACGE4U_001099"
     CDS             12221..13543
                     /locus_tag="ACGE4U_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8393280.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13698..14474
                     /locus_tag="ACGE4U_001100"
     CDS             13698..14474
                     /locus_tag="ACGE4U_001100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFF8393281.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14467..15036
                     /gene="rnmV"
                     /locus_tag="ACGE4U_001101"
     CDS             14467..15036
                     /gene="rnmV"
                     /locus_tag="ACGE4U_001101"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFF8393282.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15029..15913
                     /gene="rsmA"
                     /locus_tag="ACGE4U_001102"
     CDS             15029..15913
                     /gene="rsmA"
                     /locus_tag="ACGE4U_001102"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFF8393283.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16364..16993
                     /locus_tag="ACGE4U_001103"
     CDS             16364..16993
                     /locus_tag="ACGE4U_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393284.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17117..>18210)
                     /locus_tag="ACGE4U_001104"
     CDS             complement(17117..>18210)
                     /locus_tag="ACGE4U_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393285.1"
                     /translation="KNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGKSTTLRM
                     IAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGLKLRKYD
                     KADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMDEPLSNL
                     DAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIGTPKEVY
                     DTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGYEGKEVI
                     FGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKVDARDFH
                     KPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         5940 a         3291 c         3736 g         5243 t
ORIGIN      
        1 tattctgaaa attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg
       61 gttgcggtat gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt
      121 agttcagcaa aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa
      181 gaaatgccta cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat
      241 aacgtttatg aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct
      301 agcgagttgg cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac
      361 gcaaaatggt caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga
      421 acagtagacc cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca
      481 gaagacatta cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac
      541 tatgaattag aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc
      601 ccatcatttt tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct
      661 gcaagtgata aagctgttta taatggacca ttcacattag aagattttga tggaccagga
      721 acagatacag aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa
      781 ctttctgaaa tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa
      841 gatggccaag cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat
      901 ccagcttata cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa
      961 aagaattcac cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt
     1021 gaagctttag taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca
     1081 tcagatatgg taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta
     1141 gtttcttatg acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc
     1201 gttgattctt tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc
     1261 gaatatgttc aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct
     1321 gttccattct ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc
     1381 ggatgggctg ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat
     1441 tcatacaacc gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca
     1501 tcaacagacg ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc
     1561 atctctgatg atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat
     1621 gaaaaaatca aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca
     1681 tacgttgaag aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg
     1741 tcgcaacctc gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga
     1801 aagagcaatt agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga
     1861 cgagagcgag gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga
     1921 tcgctgaaaa tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag
     1981 aaacggcaat gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt
     2041 gatgagattt tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga
     2101 tttttttttc atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac
     2161 atctgtcaga agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat
     2221 taaaacgtgt ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct
     2281 tgatgcaatt gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa
     2341 tcgagatgct aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt
     2401 atctttctaa tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag
     2461 tcgcacattt gttagctgga agaatcggac catccttgca gttagggtta caggcaatta
     2521 tctttggaac agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat
     2581 gggcagatac atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat
     2641 ttgctgtttt attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat
     2701 gggatggctt tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg
     2761 actctgctcg gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat
     2821 tagcaaaagc aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata
     2881 gtttgattcc attgatgact ttattaggac cgttagctgt agcattgatg accggatcac
     2941 tggtggtaga aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca
     3001 caacaaatga ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt
     3061 ttgttatttt gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag
     3121 aagggagccg aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc
     3181 ctgcggatga atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg
     3241 caccttcttt gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg
     3301 ttatttcaat ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt
     3361 ctcctcatga tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg
     3421 gaatcaatat cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat
     3481 atgctcaagc aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac
     3541 gtgatgtatt aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg
     3601 ctgcattact agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg
     3661 gacgtgtcga taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg
     3721 ttgtcatgat tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta
     3781 tggccatcac aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa
     3841 aagatcaaga atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat
     3901 tcaaacatat tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc
     3961 catctgctat tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt
     4021 cagcttcatt aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc
     4081 tattatggat tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg
     4141 atggattgcg tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt
     4201 agaagtgaaa gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg
     4261 cggtgtcaat tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag
     4321 tggaaaatca gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga
     4381 tgctggtcaa atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca
     4441 aaaaatccgt ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc
     4501 gacgatgccc attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa
     4561 aaaagaggga caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga
     4621 gaaacgtatc aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat
     4681 cgcgattgca ctggtatgtt atccggaaat cctgattgct gatgaaccta caacagcgct
     4741 tgatgtgact attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga
     4801 tacatcaatt atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt
     4861 cgctgtcatg tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa
     4921 cccgcaacat ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa
     4981 cgatcgattg tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga
     5041 cgcattctat ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt
     5101 ttttgaacta tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa
     5161 agtgacaccg ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca
     5221 tacttacggt caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga
     5281 aacagtattt caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc
     5341 acatttatga aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta
     5401 ctggccgtac gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc
     5461 aagatataac gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga
     5521 tgatctttca agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg
     5581 ccgaaggaat tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg
     5641 atgatctatt gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat
     5701 tttcaggtgg gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt
     5761 ttatcatttg tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca
     5821 atttgttgca agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt
     5881 tgtcgatggt taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag
     5941 agatgggaac aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt
     6001 tatctgctat tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc
     6061 aacctgaacc agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg
     6121 tctatgcaac tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat
     6181 ctcttctggt tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta
     6241 tccgacagaa gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta
     6301 taaccatttg ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc
     6361 gctcacaccc gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc
     6421 tttagcaaat gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc
     6481 cagcttccca aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca
     6541 aacccgtacg atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat
     6601 caagataaat cagcatcggc cagttgaagg atcgattcgc tccgctacga tttctgctcg
     6661 atataatgaa gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc
     6721 tttagctaaa tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc
     6781 tctagaagta actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg
     6841 gaaattgaat gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag
     6901 caattatcag aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg
     6961 agaagattat cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt
     7021 agaatctttt ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt
     7081 aataacaaaa ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac
     7141 cgacggagca gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag
     7201 acattcggtt atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct
     7261 tgttaataaa aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc
     7321 actaattatg acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct
     7381 attgttaatt ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt
     7441 aactcacaaa gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc
     7501 acaaccttat tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc
     7561 tacaggacgt tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg
     7621 atcgatcgtt ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat
     7681 cgtgaaatct ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac
     7741 acggttgaat gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga
     7801 agactctcca gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt
     7861 gttactatga cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag
     7921 cggttcttct ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg
     7981 attaggcgta ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt
     8041 ttcttccttt gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc
     8101 accaggcaat gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt
     8161 cagctgatct tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt
     8221 gtcatattct ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt
     8281 atgttcataa tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc
     8341 attgacagta aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt
     8401 tacttgaaaa atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa
     8461 taaaaagaaa agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa
     8521 gccacaaata acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa
     8581 actgttcaaa tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa
     8641 tattaaatgt gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg
     8701 catgattgtt ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga
     8761 gaaataaaaa gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac
     8821 ctattcatcc ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt
     8881 cattttgaaa aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt
     8941 cgcatcatgc gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact
     9001 acgccaatct attatccaag cggcaaactt catatcggca actcgtatac tacgattgca
     9061 tgtgacgcaa ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga
     9121 gtggatgaac atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa
     9181 gaatatgtag ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt
     9241 tatgataaat tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt
     9301 gatcgtttat tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt
     9361 tctgacgaag aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt
     9421 aacgtgatcg gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca
     9481 tacttcttcc gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc
     9541 gaattcatcc agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga
     9601 ttagaggatc ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat
     9661 ccgaaacatg tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga
     9721 tatggctccg aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc
     9781 ggtaaagaaa tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat
     9841 ttgcctctac ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg
     9901 tctaaatcaa aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat
     9961 gcactccgtt attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca
    10021 gaagattttg tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat
    10081 cgaacgattg ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt
    10141 gtcactccat ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat
    10201 gatgcaatgg aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct
    10261 cgtgcaaaca aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag
    10321 aaagctgaat tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata
    10381 ttacttcagc caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac
    10441 tctgaaacaa tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta
    10501 gttgcaaaag gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt
    10561 caagaaaaaa tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa
    10621 gaaacagaac ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta
    10681 gaactaaaag tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg
    10741 caattccgac tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa
    10801 ttttatcctg atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa
    10861 ccacgaaaga tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat
    10921 ggctctttac aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag
    10981 aaattcaaag ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag
    11041 caattttcgt aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa
    11101 ctatccaaaa taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga
    11161 ataatactaa taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga
    11221 ttttaattga ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc
    11281 aatgacttta ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt
    11341 tctatcactt tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa
    11401 aaaaacaatc atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc
    11461 acttgatttt atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa
    11521 gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt
    11581 gatagagctt tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta
    11641 taaaccaatg attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga
    11701 aataaaaata gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca
    11761 ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg
    11821 aatacaacat cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga
    11881 acaaagtcag caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa
    11941 ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt
    12001 tggataaata atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct
    12061 tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag
    12121 ggagtttttc actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac
    12181 tgaaaattat accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg
    12241 agaaatcaat ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt
    12301 cttcttcaaa actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc
    12361 ttggcttttg ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac
    12421 agcaatcccg gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc
    12481 cagcttttta ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt
    12541 atcgattatc tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt
    12601 gttgattgca gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt
    12661 cggtgcttct gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt
    12721 catatgggga ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg
    12781 aaaagatgtt acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta
    12841 tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt
    12901 gcctaaagca attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt
    12961 tgtttttttg aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga
    13021 agcatcagac gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat
    13081 ctcggtatac ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat
    13141 ggctttagaa gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac
    13201 ggttccttat gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg
    13261 ttctttcgac tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat
    13321 ttttatcggt gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt
    13381 accactttat ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat
    13441 gacgatgatg acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat
    13501 cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga
    13561 cagaaaaagt acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa
    13621 gttcttctat tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat
    13681 gaaaaggaga gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca
    13741 atgaagatat tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg
    13801 tcgtgggctt cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa
    13861 atatatacag tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg
    13921 aaaataaatt acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct
    13981 tggattatca ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga
    14041 ttgctatcgc aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag
    14101 acacgtatca aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt
    14161 tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta
    14221 gcggtgtcgt cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa
    14281 tcgaacgatt acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa
    14341 agcgaaatga accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc
    14401 ttccgacaga agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa
    14461 gcgaacatgg ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca
    14521 agaaggctgc gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat
    14581 gaagagatcc tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc
    14641 gatcctgatt tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca
    14701 aaacatgctt ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc
    14761 gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt
    14821 tcccaaacgg ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct
    14881 ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg
    14941 aacggcaaac aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc
    15001 caagcaatga aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc
    15061 tagaacaaaa gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa
    15121 ttttctgact gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca
    15181 tacgaacgtg atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca
    15241 tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac
    15301 gatgcaaccc tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc
    15361 aacagctgtt agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc
    15421 ttactatatt acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga
    15481 actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa
    15541 agcttatggt tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat
    15601 cgtaccaaaa acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac
    15661 aagaagagat ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa
    15721 agcggcattt cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa
    15781 agatgaccag acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa
    15841 acgtcgtggg gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga
    15901 aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag
    15961 taataagaac agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttattac
    16021 cgcagatgca agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc
    16081 taagtcacaa ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta
    16141 ttacttgatg caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt
    16201 gtcacatctc ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca
    16261 ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact
    16321 aagtcgacat ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt
    16381 tcgaaaacta acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat
    16441 gcctaaaatc atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat
    16501 tgcgatgttt tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt
    16561 ctttttaaca acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt
    16621 gattggggct tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac
    16681 ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc
    16741 agaaatgcta gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc
    16801 tcaaggatat ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt
    16861 agttgattat aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc
    16921 cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa
    16981 aacgacatat tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa
    17041 tcctctaaga tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc
    17101 tctaacaggg ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt
    17161 ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg
    17221 catctacttt tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc
    17281 caagtaattc ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg
    17341 ctacttgttc actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat
    17401 aacctttttc aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc
    17461 catctgtgat aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa
    17521 taaagccggc aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt
    17581 gttggataaa tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct
    17641 gatcgtgagt aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag
    17701 cacgcatggc tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga
    17761 cttttgcatc ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata
    17821 aagcagctgg ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca
    17881 cacgtttttt gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat
    17941 caaaaacagt catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt
    18001 ctttaggtgc cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat
    18061 cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa
    18121 aaacgataaa ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg
    18181 cattgtcata ttttttatat acgtttttca
//
LOCUS       DAXWLF010000041        17825 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXWLF010000041 DAXWLF010000000
VERSION     DAXWLF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.041"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            114..926
                     /locus_tag="ACGE4U_001105"
     CDS             114..926
                     /locus_tag="ACGE4U_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFF8393286.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLLRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            923..1723
                     /locus_tag="ACGE4U_001106"
     CDS             923..1723
                     /locus_tag="ACGE4U_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFF8393287.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1884..3152
                     /gene="rseP"
                     /locus_tag="ACGE4U_001107"
     CDS             1884..3152
                     /gene="rseP"
                     /locus_tag="ACGE4U_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFF8393288.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3220..4929
                     /locus_tag="ACGE4U_001108"
     CDS             3220..4929
                     /locus_tag="ACGE4U_001108"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFF8393289.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5141..9493
                     /locus_tag="ACGE4U_001109"
     CDS             5141..9493
                     /locus_tag="ACGE4U_001109"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFF8393290.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9637..10110
                     /gene="rimP"
                     /locus_tag="ACGE4U_001110"
     CDS             9637..10110
                     /gene="rimP"
                     /locus_tag="ACGE4U_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFF8393291.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10134..11309
                     /gene="nusA"
                     /locus_tag="ACGE4U_001111"
     CDS             10134..11309
                     /gene="nusA"
                     /locus_tag="ACGE4U_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFF8393292.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11331..11624
                     /locus_tag="ACGE4U_001112"
     CDS             11331..11624
                     /locus_tag="ACGE4U_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFF8393293.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11621..11932
                     /locus_tag="ACGE4U_001113"
     CDS             11621..11932
                     /locus_tag="ACGE4U_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFF8393294.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11945..14251
                     /gene="infB"
                     /locus_tag="ACGE4U_001114"
     CDS             11945..14251
                     /gene="infB"
                     /locus_tag="ACGE4U_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFF8393295.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14278..14625
                     /gene="rbfA"
                     /locus_tag="ACGE4U_001115"
     CDS             14278..14625
                     /gene="rbfA"
                     /locus_tag="ACGE4U_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFF8393296.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14733..15237)
                     /locus_tag="ACGE4U_001116"
                     /pseudo
     CDS             complement(14733..15237)
                     /locus_tag="ACGE4U_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15402..17765
                     /locus_tag="ACGE4U_001117"
     CDS             15402..17765
                     /locus_tag="ACGE4U_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFF8393297.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6088 a         3153 c         3920 g         4664 t
ORIGIN      
        1 gaaaaaatta ttcagttttt aaataaaaaa acaatgattt cataggaaaa acagttagat
       61 attgctataa tacttaagat agtgggaatc ttttaagtat aggaggaaaa agaatgttac
      121 gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa tttaataccg
      181 atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt tgggcgcaaa
      241 accgtcgttt gctacgagtt gctggccata aagaaggcat ggaaacagta aagaaagtca
      301 caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt tcgactgaaa
      361 actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc gacttctttg
      421 atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg ggttatgaaa
      481 acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa acaaaaaaca
      541 atactggaat ggtattgaat tttgctttga attatggaag tagagcagag atcgtgactg
      601 ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa gaaatcgatg
      661 atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta caagatcctg
      721 agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta tggcagatcg
      781 catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt gcgcatcttg
      841 aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta aaaaaaacag
      901 aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg tggcgctagt
      961 cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag cagcaatctt
     1021 agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct taagttttga
     1081 aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa gatggttttt
     1141 ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta tgattctttt
     1201 aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt ttcctgtgct
     1261 tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa cagaaagctt
     1321 ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag catatatggt
     1381 tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca aaacgattga
     1441 aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt tattgctata
     1501 cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga cgattatttt
     1561 ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc attatgatgt
     1621 gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt tcgacagttt
     1681 gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat acctgcgttt
     1741 ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat actattggtg
     1801 tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa aaacacaaaa
     1861 aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc gtttttggta
     1921 tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca ggaatcctcg
     1981 tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca aaagacggga
     2041 caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca ggcaatgggg
     2101 atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat tctgacggca
     2161 tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc ccgatggaat
     2221 tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat ggagacgaaa
     2281 cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac gacggaacag
     2341 aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg cagcgtatgc
     2401 tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta ttcatcattc
     2461 tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga gagatcatgc
     2521 ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc agtgtggacg
     2581 gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat ccaggtaaaa
     2641 cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg acaccgaaat
     2701 ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca atgaatacag
     2761 gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca ttggaaattt
     2821 tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc ggacctgtca
     2881 tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt attagtttga
     2941 tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct gccttagatg
     3001 gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta agtcaagaaa
     3061 aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg gtgctagtta
     3121 catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg gtataataac
     3181 aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc aaaaatgttg
     3241 attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca aatcttgcta
     3301 agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc attagcaaat
     3361 cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat cggagcggta
     3421 gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg gcgttacgaa
     3481 acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat gatccttgga
     3541 ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc atataagaaa
     3601 ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg acctcgcttc
     3661 ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca tgcaacggaa
     3721 gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt attcaagcgt
     3781 tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg gaaagactca
     3841 aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc gactgaaagt
     3901 gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa atcgcatgaa
     3961 acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga cgaagtagca
     4021 gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat tgcggatgaa
     4081 caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa actgaaaaac
     4141 tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa atatctaggt
     4201 gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt atatgcagac
     4261 cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg attccactat
     4321 aaaaatgtga atccaaaacg cgatttcact gtgctttctt atgaagatct tcgttttgta
     4381 caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg aggaatcgaa
     4441 atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc aactgtacta
     4501 gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg tgtaagtcgt
     4561 ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg gccaaaagga
     4621 attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata ccaaacaagc
     4681 ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact ggttgatgac
     4741 agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg tccaatcaga
     4801 atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa gaaaactggc
     4861 gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct cttgaaccaa
     4921 ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct gtttttgaca
     4981 atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc aattttttaa
     5041 tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag gataagatgc
     5101 gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa aacgagattt
     5161 atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc atccactgct
     5221 tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat gggaatttac
     5281 gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc agcatctgac
     5341 tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg accagacatt
     5401 cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc aatgcgatac
     5461 accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc gtaaagtcat
     5521 cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact tgccattagt
     5581 agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag aagtggatga
     5641 gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac aggcagaagc
     5701 ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa aggaaagaaa
     5761 agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc cagccgatga
     5821 accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac ttgaggggta
     5881 cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga ctttgaaaat
     5941 cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa aagatgaaca
     6001 agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta ttcaagaaga
     6061 tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca aacatacacc
     6121 tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc atagcaatat
     6181 gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag gaaaatgggg
     6241 acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg aagcacatag
     6301 tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg tcgtagatga
     6361 tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca catacgtcgt
     6421 atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg agctagccgc
     6481 agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg atccaggaca
     6541 tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg ttcgagggtc
     6601 aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata caattttggt
     6661 agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa ggtatggtat
     6721 tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc tgtatcctca
     6781 attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct tggaacagca
     6841 tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct ttgtgaaaga
     6901 ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata tcggagaagg
     6961 agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa atcaggcagg
     7021 actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg aacgcgtccc
     7081 acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg gctcagcttg
     7141 tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg caagaaaccg
     7201 agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc ctttgatcga
     7261 gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt tggtagaaat
     7321 cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc tgaacgaaga
     7381 agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc cgttgaatcg
     7441 tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg catttcactt
     7501 tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa tcgcagatat
     7561 ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc caggttcaga
     7621 agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag atccgttacc
     7681 tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta atggcttctc
     7741 ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg gctatctagt
     7801 aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa tcactgaagt
     7861 taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt tttttgaaga
     7921 tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat gtggtgcacg
     7981 cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc atggagacaa
     8041 agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc ataactatac
     8101 gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta cagtcgccga
     8161 caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc agtttcgaag
     8221 tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa ccggccagca
     8281 tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta cgccgatcca
     8341 atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt tccactcgat
     8401 tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg ttattcggat
     8461 gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc cggaagtgat
     8521 gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt attccaaaac
     8581 agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt tagaagaaac
     8641 gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg gtacagacgt
     8701 atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg cggaagtaat
     8761 cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata gcgggatggc
     8821 atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg atgaattgcg
     8881 agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata tcgattcttg
     8941 ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat tgatggctct
     9001 tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat acttttctgt
     9061 acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg tgaaacaagc
     9121 aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga atcaactgac
     9181 agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa tgattgattt
     9241 gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg caccatttag
     9301 agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa gaaaagacgg
     9361 tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa cattgatcga
     9421 atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat tatcgttatt
     9481 cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga gtatggtata
     9541 gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac tctttttaat
     9601 ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt tgaaacagtc
     9661 acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga agtcgaattt
     9721 gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg cggtatcgat
     9781 atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc agcagatcca
     9841 gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga gcggccattg
     9901 aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc cttataccaa
     9961 ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc tgaacagctt
    10021 acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg aaaaaatatt
    10081 gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa aaaatgagta
    10141 aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca aaagaaatcg
    10201 tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat ggccaagcac
    10261 aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat gcggtaaaag
    10321 aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat gcacttttaa
    10381 tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca aaagattttg
    10441 gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt gaagcagaaa
    10501 gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa ggaatcgtcg
    10561 aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca gttctttcta
    10621 aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa gtttatgttt
    10681 cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc catccagacc
    10741 tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc gttgaaattg
    10801 tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca aatgatccaa
    10861 atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa gcaatcgtca
    10921 atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca gctgtattca
    10981 tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag acaaatccaa
    11041 aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt aaacgcggac
    11101 aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa tcagaatctg
    11161 acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa ctgcatgatg
    11221 aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca ttcaacaatg
    11281 aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag atgaaacaaa
    11341 ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa aaagagatgg
    11401 tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga aaaatgcctg
    11461 gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg gataaaaaaa
    11521 ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa cttcttgact
    11581 atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag ttaatcggca
    11641 aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca cgggtgaaga
    11701 actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg caaccgatgc
    11761 aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag ttccttgctt
    11821 tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca aggtattcgg
    11881 aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt aggaaggtga
    11941 ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat ctagtaaaga
    12001 cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg gtgcgattac
    12061 tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga aacaacaagc
    12121 tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac gtaaaaaaga
    12181 aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc gcggtcaagg
    12241 gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata cgggaaatac
    12301 cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta ctaacaataa
    12361 caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta atcgttataa
    12421 taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac caccacgcaa
    12481 attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag cagatattgc
    12541 gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc ttggtgtcat
    12601 ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga cggactatgg
    12661 catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat tcttcgaggc
    12721 agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga ttatgggaca
    12781 cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg taacaagcgg
    12841 cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg atggtaaacc
    12901 aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg cacgtggagc
    12961 aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca tgccacaaac
    13021 agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg ctgtaaataa
    13081 aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg agtatgaatt
    13141 aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga aattcggcca
    13201 aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag atttaaaagc
    13261 agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata aaggtaaagg
    13321 tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc caatcgttgt
    13381 cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc gcgataaaga
    13441 agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc aagcaggtga
    13501 ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag aacgcgcaaa
    13561 acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca acttatttga
    13621 aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag atgtacaagg
    13681 ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg tccgtgtaaa
    13741 aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag cagcagccag
    13801 caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag tgcaagctga
    13861 tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg aagaaatcga
    13921 aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg gtcaaatgat
    13981 cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct atgtaacaga
    14041 aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg taatctatga
    14101 aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca aaatgggtta
    14161 tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg taattgaagg
    14221 atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc aatcattatg
    14281 gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa tgatattttg
    14341 cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt gaaagttacg
    14401 ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc atcagataag
    14461 aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga gttagggcac
    14521 aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc agttgtctat
    14581 gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa aaatcccgca
    14641 aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt attacctaag
    14701 agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa aagaaaagta
    14761 aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat aaagatcaag
    14821 ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta tagccaagta
    14881 actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc gttgacagtt
    14941 aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg atttggtcac
    15001 gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata ccaattcaca
    15061 tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa ggaattgctc
    15121 gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca gcaaattatg
    15181 catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa gttttttgga
    15241 tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc tgttcatttg
    15301 gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa ttgggagtat
    15361 ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat tctagtaaag
    15421 aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct gttgggcaac
    15481 tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat gtcaaaaaac
    15541 atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc catttgaacc
    15601 gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga cacggcggac
    15661 aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat ccagaagtaa
    15721 cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct ggaggcattg
    15781 cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag cttggatact
    15841 ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct gctgtcgtga
    15901 ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca aatgtcttta
    15961 tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt gcaaaaatag
    16021 ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac tttaacggac
    16081 ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat cctgttatgg
    16141 ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa gctcgcctga
    16201 agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc acaccaaaag
    16261 gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc cgagcacacc
    16321 aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta ctccgttggc
    16381 tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta gaagaaatca
    16441 gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc aatggtggaa
    16501 tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa tttgaaaaac
    16561 caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc gagatcattg
    16621 agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca aaccgattga
    16681 atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca gaaaatgatg
    16741 aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac caagatgaag
    16801 ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt tacgaatcat
    16861 tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg aaatcacatg
    16921 acctttcttg gcgaaaagat tatccttcgc tgaatctcat tgcaagctca acagtcttcc
    16981 agcaagatca caatggatat agccaccaag atccaggaat attgacacat ttggcagaaa
    17041 agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg ctcgcagtca
    17101 tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc aaacatccgc
    17161 gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc ttgaaaatca
    17221 ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct gctgcaggaa
    17281 cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa ttcccaaaac
    17341 taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca agtcaagatc
    17401 cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa ccgatcttat
    17461 ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt cataaccatc
    17521 atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt gacatgcgtg
    17581 tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa atcaaaggaa
    17641 gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat caaaactata
    17701 ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa aatcttgacc
    17761 aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgtatt atttgtcaaa
    17821 ttaag
//
LOCUS       DAXWLF010000042        17250 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXWLF010000042 DAXWLF010000000
VERSION     DAXWLF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.042"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            29..466
                     /locus_tag="ACGE4U_001118"
     CDS             29..466
                     /locus_tag="ACGE4U_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393298.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            561..1445
                     /locus_tag="ACGE4U_001119"
     CDS             561..1445
                     /locus_tag="ACGE4U_001119"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8393299.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1669..4836
                     /locus_tag="ACGE4U_001120"
     CDS             1669..4836
                     /locus_tag="ACGE4U_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFF8393300.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4904..5401
                     /locus_tag="ACGE4U_001121"
     CDS             4904..5401
                     /locus_tag="ACGE4U_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFF8393301.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5420..5884
                     /locus_tag="ACGE4U_001122"
                     /pseudo
     CDS             5420..5884
                     /locus_tag="ACGE4U_001122"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5881..6639
                     /locus_tag="ACGE4U_001123"
     CDS             5881..6639
                     /locus_tag="ACGE4U_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFF8393302.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6725..7465)
                     /locus_tag="ACGE4U_001124"
     CDS             complement(6725..7465)
                     /locus_tag="ACGE4U_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFF8393303.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7526..8779
                     /locus_tag="ACGE4U_001125"
     CDS             7526..8779
                     /locus_tag="ACGE4U_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFF8393304.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9101..9328
                     /locus_tag="ACGE4U_001126"
     CDS             9101..9328
                     /locus_tag="ACGE4U_001126"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFF8393305.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9451..9777
                     /locus_tag="ACGE4U_001127"
     CDS             9451..9777
                     /locus_tag="ACGE4U_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFF8393306.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9845..10558
                     /locus_tag="ACGE4U_001128"
     CDS             9845..10558
                     /locus_tag="ACGE4U_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFF8393307.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10868..11719
                     /gene="licT"
                     /locus_tag="ACGE4U_001129"
     CDS             10868..11719
                     /gene="licT"
                     /locus_tag="ACGE4U_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFF8393308.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11840..13705
                     /locus_tag="ACGE4U_001130"
     CDS             11840..13705
                     /locus_tag="ACGE4U_001130"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8393309.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13719..15162
                     /locus_tag="ACGE4U_001131"
                     /pseudo
     CDS             13719..15162
                     /locus_tag="ACGE4U_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15270..15971
                     /locus_tag="ACGE4U_001132"
     CDS             15270..15971
                     /locus_tag="ACGE4U_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8393310.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16097..16609
                     /locus_tag="ACGE4U_001133"
                     /pseudo
     CDS             16097..16609
                     /locus_tag="ACGE4U_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(17007..17216)
                     /locus_tag="ACGE4U_001134"
     CDS             complement(17007..17216)
                     /locus_tag="ACGE4U_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFF8393311.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         5756 a         2900 c         3716 g         4878 t
ORIGIN      
        1 atcaaaaaat gcacgtagaa gtagataaat ggagtggttt tgcaaatttc ttttatttct
       61 agaagttatt cacgtatacg gatttgtttt tctcacaaat aaaaaggcag gaagtacaat
      121 ggatcatttg tatataaaat atattcatca attttccgtt ttggtgttag gcacactaat
      181 tatagcgggg attgtcgcat ttattattca aggccgtgac atattaacag tacatcaaga
      241 gtttttcagt tatgcatttg gtattcctct gatcgctata aggtctgata acaaaaagtg
      301 gaagaaacgc tattacattc tcttgctatt tttgtgtttt tatctgccac ctaagatttt
      361 tactttatta ggtatacaat gcctatcatt ttcttttatt tcctatatca ttgttttaat
      421 tttcctcagt ttcatagtat ggatgtttta tcttgtcaaa aaataaatga atgtttcatg
      481 aacagtactt catcacagcc taattttttt ctttatgcta tcgttatatt aaagaaatca
      541 atcagaaggg aaagaaagtg atgaaaaaag ttttattagt ggtaaacccc agttcaggcg
      601 gtgaacaagc aaaggaattt gagcaattag cgatagcaaa attggaatcg gtgtttgatg
      661 aagtggtcgt cttgcataca aaaaaagcag gggatgcaaa aaactttact cgcgaagcgg
      721 ctacggaagg gtatcatagt gtgtttgtca tgggcggaga tggaacagtc aacgaaggaa
      781 tcagtggaat cgctgaacaa gaacatcggc caaatttcgg atttttccca ttgggtacgg
      841 tgaatgactt ggctcgagca ttagggatac cgctagaacc tgaggaagcg atcaatcatt
      901 tttcgattga atcagtcaag ccattggata ttggaaaaat aaatgacgac tattttatga
      961 atgtcgtcgc aattggctct atccctgaat caatcaatga tgtagatgcg gagaagaaga
     1021 cgaaatttgg aaaactagct tatttcatgt caggaatcaa acagttagct tccacacaat
     1081 cctactcgtt tcacgtagaa gttgatggaa agaaggaaga aattgagagc agtaccttgc
     1141 tgataggact aactaactca gtcggcggtt ttgaaacatt acttccaaat gcaaaagtgg
     1201 atgatggtaa gctccatttg gtttatttaa aagattcttc tttgatcgat acgcttaaaa
     1261 cattgccaga cctattaaaa ggagtagatg aatctacgaa aaatctagcc taccttactt
     1321 gtgagcaatt agttgtgtca ttagctgatg aggaagaatt gacgacgaat gttgatggag
     1381 atgaagggga taaattacct gtgacaatca aagtattgcc ctcacattta caggtttatt
     1441 gttaaaatgg aggttgtgac cgaggagttg gcgggagaac accgcttgtt cgggataagg
     1501 gactgtgaca agacttttgt cacagccttt tttttgtaca aaaaagaaat ttacacctaa
     1561 tagaaaaatt tttccataaa aatacatatt ttaaatatga aagtacaatt ggaaatagat
     1621 taaaacgata aaataaaatt gtcaaaacgt cagagaggat ggtggagaat gaatcaatgt
     1681 gtaaaaaagc tgattgcgtc gattttgttt gtcttccctc tcttgatggt gacatccgtt
     1741 tttgccgatg tagagaaaac aaacgaatca acgcaatcag agaatcaaag ttttgacaag
     1801 actacttttt cagaagaatc gattacttct gaaactacag aatctcaaag tgaggcttct
     1861 actaataaaa ggcaagaaga aaataagaca agtgagacct cagaagtagc tgaacaaacg
     1921 tctgatagca gtgaagcggc actggcaaga gctgtttcag cggatgggta cagcgaagct
     1981 gccacaacag aagaactggc gcagcttctc gcagacgaat cagtaactaa gatccgactg
     2041 attcagcctt tgactttaga cagggaatta gagatcaaac gagatatcgt tattgatttt
     2101 ggtggattcg ctcacaattt cggcacacac catatttata tcaatgagat acctcagtcg
     2161 atcgagttcc agaattttaa aggaacagct gcacatccag gtggattgac accaaatatc
     2221 gatggaaatg ccattatcat tgcttatatg accgattttt ggggaagcag atatcatttt
     2281 acgggagaaa taaaatttac cgggacgctt gatttgtatg atgggtccaa attaggactg
     2341 atttacgctc ctcgtgcaac tgtcacatta gatggtgttt ccggtgtgtt ggatgtccaa
     2401 ccagtgaagg aaggactgaa tgccgctccg ggtaaagcct atttttgtag aagctatcaa
     2461 ttgaatgtga tcaatggtag tcaactttat ggaccgtatc tagggaaatt ctacggtttt
     2521 ctagattcag gagatgagac tggtagtgga aaaacagcgc caggaatcca tatcttaagc
     2581 ggttcaaagg tctctcttga ttatgatcgg gcagatggcg ttagcgatga aggagaggca
     2641 gtcgatacgc tacctggtaa tgtcatgttt gaagtcagtg gttcgggaag cgaattttcg
     2701 gtaaatacaa agatcaatat taccaatgac aacaatcgcg gaatcatcca aatgagaggt
     2761 tcaggatcga aagtcactgt atcgaatggt gggaaaatca ccatcaatac agcaactacg
     2821 ggcggatttc gcttgcaagg agattctagt caaatccatg tatcaagtgg tggacaaatc
     2881 tcggtcaaaa tggctggaga tgacgatcaa cccgggaaca atgggatgcg ttttgtagga
     2941 aaaggccttt ctttgatcgt agaaggagcc ggatcagtta tcgacatcga taagcagagt
     3001 gggcgttcca gcgcagttcg ttttgagaat ggtacgcaaa agctgacagt aaccaatgga
     3061 ggctcattga gggtaagaaa tacaggtgat ggcaaatctt acgtgaacag actcaatcaa
     3121 ggaaatcaag cgatccaatt ttatgacgct tctggttttc tagaaagtcc aggttcagct
     3181 gattttacag tcactggaga aaaatcaaat atcgatatac gcgcagattc cggagcaact
     3241 gtagatacaa ctggagatat tgatttaaat tttacggcag gtgaaaaaac ttatttagtc
     3301 atggtcggga aaacagggga agataagacc ggcatcatca gtggggatgt gttgaatgtg
     3361 gacttagaat ctcctaccta ttttgatttc caaaacaaac ggattggaag cgagaccaat
     3421 ggcataggcg gctgggtatt ccaaggaaat ggagattctt ctttaaagat cgcccattca
     3481 gaaatcgctt tatggcgaaa aacaggcgca acattcgata actttgaaaa agacccggat
     3541 tatttttctg atatgacaga cttatcgatc aatgggaccg acttaggcaa tttcgtatca
     3601 agccctagtg caggtttaac aaatgaattt gctcagacag gtgccggaat gaaaaattat
     3661 aatcgaatca gtgccaataa tcagctcccg atgattgaat ccttaagagt gccaacgaat
     3721 gcagataaaa aaatctatgg gcatgtcgtt gttcccgaag gtgtcgaggc aaagccaaga
     3781 gatgcttgga caggcgaagt agaagtgact ttacgtatta cttacgcaga tacgacgaaa
     3841 tcatctgtag aaattactgc tctcacgcaa ggacaaacag atgaaaatga aggggcagga
     3901 tataacccat ggggagaagg cgaacaaggc ggcttgttcg aagttcaagt acccaatgat
     3961 gagagattaa aaacggagga tcaagtgcaa gttgttgctg ctaaaaaagt aagaggtggc
     4021 caagaagtga cagaactgga ctctgccaaa acgacagtcg atgtgattcc tcctgaacct
     4081 gctaatttaa gtaccacatt gatcaatgca gcttctcgca cgatcacagg tacaggtgaa
     4141 gcaggagcct tggtttcatt gaaaaaaaat caagctgttt tgtcagaaac aactattgat
     4201 gatacagggg aatttgttct ttctatccca gaaaatcaac tttttgcagg agatgtatta
     4261 gaaattgtat taaacgacca agcaggagaa gctactgcta agggtgtgca gaacaagcca
     4321 agtaccaacg atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat
     4381 gccccgcctt ttgcacctgc tacgaagatc gtagtagaag gtggtctctc atttcttgcc
     4441 ccttccaaac tcgattttgg tcagatcaaa gcaactggtt tgacacaaga agcatttggc
     4501 caaatggata tacaggaaaa attatggatt tatgatcagc gagaaacgaa aaatccgtgg
     4561 atcttaacga taaaattatc tcagcctttc acaaatgatg caggattttc tatgcaggat
     4621 gtcctatatt tcaagaaagg agaggagtat caattagtga atgaagaaga actggaaatt
     4681 gtcagaggac aaaattcaac gaatgtgcaa gcagattatt cagaatattt aaataacggt
     4741 cgggcgttta aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag
     4801 ataacatgga cattagcaga tgcgccgaca gaataaagat caaggaggat gattgatgaa
     4861 tcgaatatgg aataaaagtc atcgaaacaa ctgctcttta cttatgatcc ttctccttgg
     4921 attcatcctt tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga
     4981 attaccggct aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg
     5041 gaaaacacag gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga
     5101 gacattgatc cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa
     5161 aagtaaattt gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga
     5221 aataactgtt cctcctcaaa gtgcggtcac tgagacactt tcactgaaag cgccagaaaa
     5281 agagctggct ggtgtggtcg caggagggat cacgtttcaa gaaaaaaaag agaacgtttc
     5341 taacaaaagt acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgatttg
     5401 aaatagcgaa aaagagtctt ccccagattt gaaattaaaa aaagcttatg cggatcaaag
     5461 cgatggaaga aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg
     5521 cttgactaca tcggttgaaa tctacaaaaa agaaacaaaa gagccttacc tttcaataaa
     5581 aaaagaaaaa atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg
     5641 aaaaaaatta gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg
     5701 ctggaatctc cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa
     5761 ggatattgat ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg
     5821 agtcattctt attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa
     5881 atgaaaaaaa caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa
     5941 agtgtttttg ctgagaataa gagctcagcc actacagaag gaacgatttc tgtgaccaaa
     6001 ccagatgctg caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatcct
     6061 gatactcctt ttacacctga cgaaccaaat cagggaacat ccggattact gacgattgac
     6121 caagctccag atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct
     6181 gcattacaaa gcggaacgga cagtactgga gcacaaacac aagtacagaa tttcgtacaa
     6241 gtaacggata aaagcggaaa ttatgccggt tggaaattat cagtcaaacg aacagaattt
     6301 attggtacaa catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc
     6361 gtgattcgaa atagcgcaac taatagcagt ggaatgccat ctaccgtttc tggaactggc
     6421 gctgaaggaa tcgaaatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat
     6481 gaagggattg gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca
     6541 gtggaattat ttgttcctgt atccaattat gctgtagaca actacgtttc aacattcaca
     6601 tggtcgttaa cagatgctcc tacagaagag gaagcgtaaa atcatatcaa cgatttcagg
     6661 gagtggagcc gattcaaggc ccactccctg aaatctgcta tgcttacgtc tcagctctta
     6721 ttcatcaact cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga
     6781 tctttcgacc aacgagtaag gagtcaccgt tgaaaaattc taacacataa tgagcagaat
     6841 caattttctg caattgcgat aagttgatac agtaagattt taaaagtaaa aagtaagcat
     6901 acttttctaa ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag
     6961 ttgttttgac cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc
     7021 gaggatacag gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga
     7081 tctgctgttc tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc
     7141 tgtcttttct gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat
     7201 tcgtataagc ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg
     7261 cgacatcaat acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat
     7321 tatcaatagt gcccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga
     7381 tattctcgat atgatgattg accaattctc tgatgatcca ttcgctgtga aactggttat
     7441 cttctatgat aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt
     7501 tgaaacaaaa caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac
     7561 tttgatttta gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag
     7621 ctttggttgt atagctttga tggtggtgac acttttactt cctatcccta aagtgagctt
     7681 tccttaccta gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt
     7741 tatcagctgt ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt
     7801 ttttgatctg atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact
     7861 acttatcgca tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct
     7921 caacttgctt tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag
     7981 ctggggcgtg atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct
     8041 tctatctctt ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat
     8101 cagtttccaa gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga
     8161 agaatattac catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt
     8221 aaaaattact cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tattagaaga
     8281 tcattctgca gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc
     8341 agctgtcagg ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat
     8401 ccaagtagtt attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg
     8461 ctgtttatct atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat
     8521 tacgatcaat ctgctggaag aaaaagaggg atcacgtttt agtatagcga acaaggcaaa
     8581 tccaacgaat ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg
     8641 cgggttaggt ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa
     8701 aatcgaattt tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa
     8761 gagcaaatca gcctactgac ttactgtcaa aggataagtt tgaggaaagt ctacacggag
     8821 atagagacaa caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct
     8881 tctgtttctt ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag
     8941 tacgaaagta tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg
     9001 tcaaaatgct aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta
     9061 ggcgtggaga agcaagcaaa tccctacgat cacaaaaaaa atgctgaccc agcagttaag
     9121 agagttagaa caagatgaaa gtatccatcg aaaagcctat aaccaagtcc cgcctaaagt
     9181 agagtactcg ctcaccgaag aaggaaaatc attaagagag attctagtag cgttgtctgt
     9241 ttgggaagaa gaacgtatcg aaaaacaaaa agcctcaagg gaagaagaca cgctactgca
     9301 tacacatggt ggctacctaa attattgatt ttctatgaca tttattttaa ataaaagaaa
     9361 acaggagttt ttttaaaagc atcatctatt ttttatgtaa aaaaatggta tgattaatca
     9421 agtaagaagt gattggaaga aggcaagcaa atgaatagta tggaaaaagt caaccagttt
     9481 agagatgaac gtaattggcg acaatttcat aacgaaaaag atttggctat ctctatttct
     9541 ttggaagcat ctgaattgtt agaacttttt caatggaaaa cgccggaaga agtaaaagaa
     9601 acacaattag aacgaatcaa agaagaactg gcagatgtat tgatctattc atacatgatg
     9661 gcagacaacc ttcacctgga tctagatgag atcattgaag aaaaattgat caaaaataat
     9721 ttgaaatatc ctgtagaaaa aagcgcaggt gtacgaaaaa aatacacaga gttgtagaaa
     9781 tgtgtcattc ggctgttgga aagagcaaaa gaaaaacagt aaaatgagtc tggagggaga
     9841 gaaaatgaaa aaaaaaggaa aagcaaaaag atggctagtc aatatcctct tgttcctctt
     9901 gttattagta ggactagctt tgattttcaa tgaacagatc aaggattatt ttgtcagaga
     9961 gacaggagat aaatatgcta tagcgaatgt aacaaaagaa gatctgaaaa agaataacga
    10021 taaagatgtc agttttgact ttgatgcggt agaaccaatg acgacagaag gggtcatgag
    10081 gtcgcaaatg agaggaacag atctaccagt gattgctagt atcgcagtcc cttcagtttc
    10141 aatcaattta ccggttttca aaggcttgga taatacgtca ctactttacg gagcaggaac
    10201 attatctcca gatcaagaaa tgggaaaagg aaattatgcc ttagcaagcc atcgtgcaac
    10261 gaaccctgaa ttgctgttta caccgctaga aaatttagag atgggtgcca agatctattt
    10321 gacagattta gaaaatgtgt atacatacaa aacatttttt aaagaaaaag ttgctcctac
    10381 agatacccaa ttactgaatg aagtcgaggg aaaagaaatt gtcacactaa ttacttgtgg
    10441 cgacatggac gccgtcacgc gactagttgt acaaggagaa ctggaaagtg ttacatcgat
    10501 aaaagatgcg acagatgata tgagatctgc tttcaatcta gagacaaaaa cattttagga
    10561 tactgggaca taagtcgtta cagattaaaa acagccaaag gatatgaatc gataaggaaa
    10621 aaccaatttg ctgaaaaaaa gagaaacgca aagaggtgca tctctgattt ggttgttttc
    10681 tagatagact tgtgttccag tttttttatt ttttatatga aaaggctcgc ataaataaaa
    10741 attcgatggt atgatgattt tgaataagaa atggattgtt actgtacggc aggcaaaacc
    10801 agaattcagc taagaaacag taggcgaatt ctaggttttt ttatttttct tgagagggcg
    10861 aagcaaaatg aaaattgata aaattttgaa taacaatgtc gtcatttcga aaaatggatt
    10921 cggggaagaa gtcgtttgta tgggaaaagg actagctttt caaaaaaaga ttggggacga
    10981 gatttcgcca gaagcggtcc aaaaagaatt tgttttaaga gattcttttg ctaaaaacca
    11041 attccagcag ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt
    11101 cgaactggct gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac
    11161 cgatcatctt cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt
    11221 attatttgaa acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt
    11281 gttcgtgaaa caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt
    11341 tcattttgtg aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac
    11401 aatggtaaga gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga
    11461 atctttgaat tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa
    11521 aggggaagga tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc
    11581 aaaagcgttt cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga
    11641 aatggggatc gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa
    11701 aaaaagcatc caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa
    11761 taggttcacg aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa
    11821 aagaaagaag gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt
    11881 aggaggacaa aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt
    11941 aaaagatgaa tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt
    12001 cgtacaaagt ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca
    12061 atcgatcgtg gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa
    12121 tattttgaat cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat
    12181 ggcagccgcc ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac
    12241 agctgatagc ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt
    12301 acctgtcatg cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt
    12361 agcaattgct atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc
    12421 tatcagtttc tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat
    12481 accaatcatt ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat
    12541 tccgcaattt ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt
    12601 aacatttctt gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt
    12661 caactcgatt tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca
    12721 agtattcgtg atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga
    12781 acaatatggg tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg
    12841 ggctgcttta gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc
    12901 ttctacagtg acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc
    12961 tttgaagaaa ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg
    13021 attttccgga gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt
    13081 cattaatacg gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat
    13141 tgcttttgtt cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga
    13201 caatcaatta gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac
    13261 cttaaaaagt cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt
    13321 ttcttctggc gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc
    13381 acctgcagac ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac
    13441 gactgacgga gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa
    13501 tggctttgag atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg
    13561 ttttgacatc gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac
    13621 gaatactgat gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga
    13681 agatgttctg gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa
    13741 aatttttatg gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac
    13801 ggaaaaggac tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc
    13861 ggcagtgaag aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga
    13921 ggaatcgatc attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc
    13981 aaatgttatc gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact
    14041 cctaacgaag cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat
    14101 attgaacctg tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac
    14161 ggtggctgga aaaatcgaaa actgatcgac ttctatgaac gatttgctca gaaagtatta
    14221 gagagataca gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt
    14281 cactttccag ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat
    14341 attttccaag catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac
    14401 gaattgcgtt cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc
    14461 gatgccaatc cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc
    14521 tgcacggatg tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa
    14581 tatggagtcg atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa
    14641 tatccagtgg actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat
    14701 ccacaagcag caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag
    14761 gcaagtgaat ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta
    14821 tacaatcgtt accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat
    14881 atggaagagg atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc
    14941 gaagcgatgg ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga
    15001 tgtatcgatc ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat
    15061 gtggatctag acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat
    15121 tggtataaag aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa
    15181 aaagaacaca cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt
    15241 ttagtaatag taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag
    15301 tcgcagataa aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc
    15361 cgacagaaag tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa
    15421 tcgaactgct ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc
    15481 taagcaatcg tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga
    15541 actcccgata tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg
    15601 aagatccttt gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt
    15661 tgaacaatca gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca
    15721 gcagagaaga atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta
    15781 ttgactcatt ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc
    15841 tgaaaacaga cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg
    15901 aaatgattga atattcggag gcggtctacc atacggagct gcatccttac catattgatt
    15961 atgaaacata aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
    16021 agccaaacaa aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca
    16081 cggctgtttc tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg
    16141 aatctatgaa ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac
    16201 gatcgcttat ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga
    16261 tacattgaag accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat
    16321 cgtattgaac gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg
    16381 cggattaagc atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt
    16441 cgctacgatg accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac
    16501 aggaataacc attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga
    16561 agaaaaagag gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa
    16621 tggattacaa gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc
    16681 agagttagaa agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag
    16741 catcggcacc ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag
    16801 ggtctggtat accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa
    16861 aaaggaaaga aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt
    16921 acacgttcga gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca
    16981 ctgaattttc ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc
    17041 gttaacaaaa agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt
    17101 agttcgtcgg cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac
    17161 aaaatgattt tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta
    17221 ttggcatata ggacgaaatc ccagtatctt
//
LOCUS       DAXWLF010000043        17204 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXWLF010000043 DAXWLF010000000
VERSION     DAXWLF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.043"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(119..478)
                     /gene="comGG"
                     /locus_tag="ACGE4U_001135"
     CDS             complement(119..478)
                     /gene="comGG"
                     /locus_tag="ACGE4U_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFF8393312.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(450..875)
                     /gene="comGF"
                     /locus_tag="ACGE4U_001136"
     CDS             complement(450..875)
                     /gene="comGF"
                     /locus_tag="ACGE4U_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFF8393313.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(838..1185)
                     /locus_tag="ACGE4U_001137"
     CDS             complement(838..1185)
                     /locus_tag="ACGE4U_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393314.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(1157..1627)
                     /gene="comGD"
                     /locus_tag="ACGE4U_001138"
     CDS             complement(1157..1627)
                     /gene="comGD"
                     /locus_tag="ACGE4U_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFF8393315.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(1602..1880)
                     /gene="comGC"
                     /locus_tag="ACGE4U_001139"
     CDS             complement(1602..1880)
                     /gene="comGC"
                     /locus_tag="ACGE4U_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFF8393316.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(1877..2926)
                     /gene="comGB"
                     /locus_tag="ACGE4U_001140"
     CDS             complement(1877..2926)
                     /gene="comGB"
                     /locus_tag="ACGE4U_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFF8393317.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(2883..3812)
                     /gene="comGA"
                     /locus_tag="ACGE4U_001141"
     CDS             complement(2883..3812)
                     /gene="comGA"
                     /locus_tag="ACGE4U_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFF8393318.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            3944..5698
                     /gene="ade"
                     /locus_tag="ACGE4U_001142"
     CDS             3944..5698
                     /gene="ade"
                     /locus_tag="ACGE4U_001142"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFF8393319.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(5796..6458)
                     /gene="trmB"
                     /locus_tag="ACGE4U_001143"
     CDS             complement(5796..6458)
                     /gene="trmB"
                     /locus_tag="ACGE4U_001143"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFF8393320.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(6528..7310)
                     /locus_tag="ACGE4U_001144"
     CDS             complement(6528..7310)
                     /locus_tag="ACGE4U_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFF8393321.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(7514..8725)
                     /locus_tag="ACGE4U_001145"
     CDS             complement(7514..8725)
                     /locus_tag="ACGE4U_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8393322.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(8718..9455)
                     /locus_tag="ACGE4U_001146"
     CDS             complement(8718..9455)
                     /locus_tag="ACGE4U_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393323.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9593..10027
                     /locus_tag="ACGE4U_001147"
     CDS             9593..10027
                     /locus_tag="ACGE4U_001147"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFF8393324.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            10020..10364
                     /locus_tag="ACGE4U_001148"
     CDS             10020..10364
                     /locus_tag="ACGE4U_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFF8393325.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            10544..11554
                     /locus_tag="ACGE4U_001149"
     CDS             10544..11554
                     /locus_tag="ACGE4U_001149"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8393326.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(11623..12567)
                     /locus_tag="ACGE4U_001150"
     CDS             complement(11623..12567)
                     /locus_tag="ACGE4U_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8393327.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(12551..15271)
                     /locus_tag="ACGE4U_001151"
     CDS             complement(12551..15271)
                     /locus_tag="ACGE4U_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8393328.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(15268..16488)
                     /locus_tag="ACGE4U_001152"
     CDS             complement(15268..16488)
                     /locus_tag="ACGE4U_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFF8393329.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(16769..>17204)
                     /locus_tag="ACGE4U_001153"
     CDS             complement(16769..>17204)
                     /locus_tag="ACGE4U_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFF8393330.1"
                     /translation="LEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKANIFN
                     HLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMF
                     ESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
BASE COUNT         5286 a         3441 c         2876 g         5601 t
ORIGIN      
        1 atcgaacaat tgacaaaatt tcaatcgttt tcactgtttt ttatttgtga aacggcaaac
       61 atttttttat taaaaacagt gttataacag aaaagagggc gttttcacga acattccatc
      121 attctgtact agtatctgta tcatttttta cttcttctgt gaaccgaaag ataaacggtg
      181 agacttccac ctctaagaat agggactgtt gtacccactg atatgttaca gatccagttg
      241 tgtagtaaaa aactccttcc gaactttcgg caagcgtaga ctgttccgat aaagccatct
      301 gcttcattat ttttgctata taataatttt tgattcgtat attaagatcc attgacaaac
      361 ggtagctttc taatgctgcc atcaataaaa acgtacataa aagaaataaa agcaagacag
      421 aaaataaaat agagccctta taacattttc tactgatcgt tttttgtcca tatggcatat
      481 ccatcttctc cattttcaaa gtgtacataa aaagaaattg tgtgttcttt ttccaataat
      541 tgaagctcag tgatacttgt taacataggt tgatgtccgc cggaagttct tcttttacgt
      601 atcattgcct gatatctttc gatgatatac tgtttatcct cgatcgtgta gtgcagttgc
      661 tgatttttca cagagacatt gtgactgctc ttgagttctt cttctaattg tgctaaaaat
      721 attagccact ctttttgatc tttacgctgc aaaaattcac ttacttggat ggtttgctga
      781 atcaaaagat tgaatgtgag caatagactg ctcaatatca tcagtccaat tagacattca
      841 atcagcgtaa atgctggaat tacttgtttt ttcaatactg atcgcttctt tctccatatt
      901 gacaaacact ttttcgatta ctccattatt atcgacaatt tggatatcgt atgggatcat
      961 ttcttccctt cgctctggtc ttttgtgata catccgctgc gtcttgatat cttcataaat
     1021 cattcgaata aatagcagtt cctccccact tttcttttct gcctgaagca aaaacagctg
     1081 gccaaagctc actaagtaga cacagacaga agcgatcata agcgctgtaa gggactccaa
     1141 caaaacaaat cctgttttat tcaattcttt ttgtatagcg accactccct atctggaatt
     1201 gatacgtaat cgtccgctct ttttcttccc aataaaattg ataagctttt agtgaacttt
     1261 cattcccggt tctagcagca aaagtaatag aagcatggcg tttcaaggta atctcatcag
     1321 gaatatctaa tacttcccaa tttattttat ctggattcgg aacatgaaaa atgatttgat
     1381 tctcttcact attccaataa aagcctgttt ggatctgatt cacaatagcg attttttgcg
     1441 tagaatagat tcgtttttca aattggccga aaaattgata gactgccaat tcattcttcc
     1501 atgcagaaaa tgcgagaact ggaaaagaaa ggatcgttgt aagtaataaa atcagtaaga
     1561 gtgattcaaa caacgtatat cctgatgcta aaaccttacc ttcaaggccg ctgatagatt
     1621 tctgcatgtt ctcgtttgat atagccttca ttcaacaatt cctccacagt aggaactctt
     1681 ccattatttt gaagcgcata gagctctttt tgtgtgtcta ccaactggat gatcgcttca
     1741 cgactttctt ggtctacatg attcctataa cgcgacaaat tagggacgaa caacaacact
     1801 aatacactga tgatcagtaa gacgattagc atttcgatca atgtaaaacc gctataataa
     1861 gatatttttc tctttttcat ggcaaaacct cctctatgct tccgtaaaca ggtaataaaa
     1921 cagcggcata aactaataaa atcaaagccg ctacgcccaa aaaaatgatt ggctgaataa
     1981 aatgcatcca gtgatgaaga cgtttgaaaa gctgttttct tgtccattcg ctgtaaaaaa
     2041 tcagttcttt acctaaactt ccctttgcct ctccctgtaa aacaatttta ctgaattctt
     2101 ctgatagaaa acgataattc tggaactgct cagccaatga catacctgcc tccaatcctt
     2161 gaatcaactt agaggcaaga tgctgaatca gactttcttg tttcgtttcc tttaagcaga
     2221 ctatgatttg ccttaattcg aaaccttcat gaaaaagctt tccaatctct agtgacaaat
     2281 aagccgattg atacaaggaa tatatcggtc caatcaataa ttgtctgctt atccattcac
     2341 tctgatcaac aggagacaac ttcatcagac gataccttac tagtaaagcc aatagagtaa
     2401 ccgctaggat gaatagtaga acataccaat gaaagacttg tatcagctga ataccccaat
     2461 gctcttgcat gaccattcct gaaccaataa gctgaggcaa aacgaacaac cgcatactaa
     2521 acaagatacc tagcaaaaag gtgagcaata gcatcggata tgcaagcagc tttttcaaat
     2581 cattgcgaaa tccttcaaca agccgaaact gtttggcaat ttccaacaaa gttgtcggca
     2641 gattcccatg acgttcagct aactcgatct gtaccagttg ctctgctcca tatcctattt
     2701 gcgaaagtac ctcactaaac gcttgccctt cctttagctg atgttggata tttgccaaaa
     2761 taacaggtgg aaacgaacgg atcgtgagta ataaataaag ggattcttgc agactaaaac
     2821 cagataaaag cagttctccc atggtttgtg caaactggtt ttgctggctt cttgttaatc
     2881 ctttagaata gttctgactg ccaaatattt tcctgagctt cttcataact ttcctcccat
     2941 gattttttta ctgattcttc ctccataaat tgataactcc ataaaacaga aggatggtga
     3001 ttggcatctt ccaaaagttc ttgataaatg acaccagcta aacattcaga tagctcttct
     3061 tttttctttg tcagttcatg gattctgacc aatgtacctt caagattgcg tgcatgtacg
     3121 gttgcaaaaa cccggtgccc tgttagtgct gctcttaccg cagcatgtgc agttgattca
     3181 tctcttatct cgccaatgat tagcaagtct ggacgatgcc gtaatgcgag tctcaaaagt
     3241 gtatcataag tctgtgctat tttcggattg atctgtagct gcagaaaaaa cggttcttct
     3301 atctctacag gatcttcgat agtaatcact tgcaattttt cttccaaagc caaccgatac
     3361 attagagaag tcttgcctga tccgactggt ccgctgaaaa ggtataatcc acgtctgacg
     3421 gttttctgta aaatagttgc aaaatctcga gaactaaagc aacgatgggg tgtttcaaaa
     3481 gtaccgtata atagccgaat cactaaactc tctcgttgaa ggtaatctcc tacagaagac
     3541 aaccgcagac gaacttcctg tttttctaaa gaataagtaa atgcacctaa ctgagctttt
     3601 cttttttctc cgatatccat ctgtcccaaa tacttaaatc ttgagatcag ttgctgtcca
     3661 tctgccgaag agactttctt ccagaatgtt cgtttttttc catagcgaaa atagatgtaa
     3721 aatacttctg aagctggcaa tatatatacg tcttgggcat gtttggcaaa actttcactt
     3781 atcatttgat ccgataatcg ttttatttcc attattctcc cctccacaaa aaacatacgt
     3841 ctttttgatc tttttgtttc aaataaaatg aatttacaaa aaaatattgg caaaaaccga
     3901 atttatgtta tacttataaa aatttatctt gggaggcact ccattggaaa caacattaag
     3961 gaaatatatc gaagtagcaa gtaaaaaaaa gcctgctgac ctagtgatca agcatgctaa
     4021 gatcgtcaac gtctttacga aagaaatcat ggaaaaagat gtagcaatct gcgaaggaat
     4081 gatcgtagga atcggtgaat acgaaggaaa aagcgtacat aatgctaatg ggcaatatct
     4141 agtgccagga ttcatcgatg gccatgtgca tatcgaaagc tcacttttat caccaagcga
     4201 attttcaaaa gtatccttgc ttcatggagt tactactgta gtgacagatc cccatgaaat
     4261 aggaaacatt gctgggagta caggtcttga gtttatgata gaagatgcaa gacaaacact
     4321 catgaatatc ttttttatgc taccttcttg tgtacctgta accccttttg agaccaatgg
     4381 tgcaaattta aatgcaaaaa aattagcccc ttttttacaa aaacccgaag ttttaggctt
     4441 agctgaagtc atgaactatc aagcagtagc aaataatgaa acagacatcc tagaaaagat
     4501 ccaattgatg caccagcata agaaaaaaat tgacgggcat gcagctggta tcggcatgga
     4561 agagttaaat gtttatcctg cagcaggtat tcggacggat catgaagcta caactgcaaa
     4621 agaagctaaa gaacgtttgg atcttggtat gtatttaatg gttcgagaag ggaccgttgc
     4681 aaaagatctg gcatcgctac taccggcgat cacacctgaa aattcaagaa gatgcttctt
     4741 tgtcaccgat gataaactga tcaatgattt agtaaaagaa ggatcaatcg atcatatcgt
     4801 ccgtcaagct attgcacttg gaatcgatcc attacaagcc attcagatgg caacattgaa
     4861 tgcagcagaa tgttttgatt tgaaaacact aggtgcgatt gcccctgggt atcaagctga
     4921 tttcttctta acagacgatc ttaccaagct gccaattcat gaagttttct ataagggaac
     4981 attagttgtt caatctgggc aactccaaga ccatttattt gctgatcatt caaattcata
     5041 tacgtgttgt ctgccaaaac taaacgctca accattaaat ccacattcgt tagagctgct
     5101 tctttcttct tcacaagccc atgtgatcga aattatcccg aatagtctag tgactaaaga
     5161 tttggtagaa aaagtggacc gacaaggcga acgattcatt ccttcaatcg aaaaagacca
     5221 attaaagatt gcggtgatcg agcgtcacca tcaaacggga aacattggat taggcatagt
     5281 gaaaggattt cagctgaaac aaggagcgat agcgacaacg attgcccatg actcgcataa
     5341 tctagttgtc gttgggacaa acgacgagga tatgctgtat gctgctaatc ttttgattca
     5401 aaaaggtggc gggatgaccg ttgtcaacaa acagcaggaa atagcttgtg tttctcttcc
     5461 tataggcgga atcatgacac aggaaccttt ttcgatcgtc tatgaacagt tgaatgaatt
     5521 aataactaaa gcacatcaac taggagcagc taaaaccttt gatccttttt tgacactttc
     5581 tttcttaaca ttatctgtca tacccgaatt gaagatcacc gacaaaggat tattttcatt
     5641 ttctcgcttc caattgattc caccttgtgg aaaaaccgat gaaataaata cattttaaat
     5701 caaataaatc ccagaaaata cattacatct gtaatgttca ttttctggga tttatttatg
     5761 gatattaatg tttttatgaa agcagaaagt tagtttcaca gacttttctt tttggtctta
     5821 tcttgaaatt gagcttctac tcgataaata cgttgacctt ttgaagaaaa tttttcttca
     5881 tactccgtca taatattgcc ttcaaattga ctttgatgta aatctaacca tacctgtttg
     5941 aggatcatgc catattgaga aaaacttgcc agagaatact caaacaatcc ttgattatct
     6001 gttttgaaat ggatttctcc atttggacga agaatctgtt catctacagc aagaaaagtc
     6061 ttatatgtca atcgccgttt ttcatgacgt ttttttggcc atggatcgga aaaattcaga
     6121 tagatttgat ctacttcgct gtcagcgaaa tactctgtca acgctgatcc atccacatgt
     6181 agaagtttca ggttaggaag cggttgttcg atcaacttgt ctagtgcaat cgaaacaaca
     6241 ctgacctgca tttctactcc aatataattg atttcaggat gggcttttgc cattccagtg
     6301 atgaactggc ctttccccat accaatctca atgtggattg gatgatcgtt ttcaaacaat
     6361 tcattccatt ttcccttcca caatgttgga tcactaatta caaaattcgg gtgagccgct
     6421 aacatttccg ctgctcccgg acgatttcgg acacgcatta tttatctcca ttcctttcct
     6481 ctaattaaaa aggtctggcc aaatctccgc agagtttggg ccaaacctca gccactgaaa
     6541 attcgtttta attgtaaaat ttcttcgttc atccgtcgat gatcttcacg tttgcaatat
     6601 ctggtgattt cttggagcaa attcatggcc gcataccaat gaaatttttc aagcatttcg
     6661 tcagtcgggc gtatgccata actgatcagc cactgattcc agctagacag tggcacataa
     6721 tttcctaaaa tcgttgcaat atccaccgca gggtctgcaa acatgattga atcccaatcg
     6781 actagatata aataattttt acatactaac caattacgat ggtttacatc tccgtgtact
     6841 actgataagt tttccttatc tattgacggg atctgctcac gaagaaaacg atggacaatt
     6901 tgcagaaatt gatttttctg gagttcttcc ggtaattctt tttcatattt ttgcagcatc
     6961 ttctccggcg tcatgacacg accaccgata cgggtcagca tggttttaag agaatttgaa
     7021 tgatgtaaat ggtacaaaac atcaatcacg tcgtttcttt gaccaatctc attaggagtc
     7081 agcaagcgac cttccagcca ctcttgtgct gtcaaagtat cgccattccc cgttcgtttt
     7141 gtccaaacaa gttttggtgt aattccttcc cttgaaagag cagccaacat cggagtcgtg
     7201 tttcgtttaa taaagacttt gtcctcatca cgcatgccta tgtatgtttt tcctgtgtcg
     7261 cctttgatgg gacgtaaccg ccactcttgg tctaactgaa actccatcat gcattctccc
     7321 gtcctcttac tctttgtttt gcagtactta ttattctagt cgcctgaaca ctacacgtca
     7381 agcacatata cgttcttttt catttaattg ccattcaaaa aaccagcttt tctcaaaaaa
     7441 aatagaacga acagcattct tactgtcaac gctctatttt tcattctcat ttttacccta
     7501 cacagctttg ttttcagtct gacatcttct tcaaacgagt tggtacatat agtttaacaa
     7561 agagcatagt aacaatcaaa taagccgcta gcggaaccca tcgctgctgc ccatctaata
     7621 cgactgcagc aatcaaacca aaaataaccg acgcgataat taatacccat gtgagtagat
     7681 actgaatcgc ctgcttcttc tgattcttat ggattggata aagctgaacg agaaccatat
     7741 agcggaactg gttcgctaaa ggtaacagtt ggaaaccgat caaatataaa aataagaaac
     7801 cgataatcag actaaaataa aagtcgacta ctccggcaat caataaccca cctataatta
     7861 ctaatcggaa ataaagacca ctaaaatcag aacctcttgc caatcttctt gcaaacaagt
     7921 aaagataagt gttttttgat tctcttttaa tatgagtcaa tataggatcc aaatagcgtc
     7981 ttcttttgat ctttgccgta atttcaggaa catctgtgaa taaattgata aattgataga
     8041 tctgatgcaa cctatgttct tctacatgca ccattttctc ccaatccaat cctttttctt
     8101 gttgcttcca caaaaaccag taatatagac ttgcctgtac taatgcacct gctattcctg
     8161 cccatattag ccaataaaat gatagaaaca aaaagatcag agaagaaaaa aaccagccta
     8221 atttccaata acgaataact tgttgatcta gtcgaaacaa ttctgctctc ttgatttgca
     8281 gatgactgaa tttcaaactc cataatatga tcagatagac gaaaaacatc gaaaaggatt
     8341 gctccgtact aaccaccact aatggcatgg ccaatccgca tgccaatatc aaaaagatca
     8401 acggaaaaat gcaagaatag cgaaaagctc gttccaaata ggtttgcatt tctttttctt
     8461 taggaagtaa aaaaacaata tctgctgcct ctgcaaaagt agccatctta cccacgaata
     8521 aacagagcat ccaaaataca gagattatga gccctccaat tgcaaacggt ggctgcaggg
     8581 ttttcaacca attagaataa tataatccca caccgccaat caagaaaaga caaactaaaa
     8641 caaaatgatc gttcaataca taacgcatat atttcatcat tttcttttga tgtcttgcta
     8701 aacgtttttg aaaaaaacta ctcatgtgcc tgctcctttg ttaaagcaat atagatttcg
     8761 tccaaagaag aatccggcag ctgaaattct tttctcaaca tctcaagaga accgtctgct
     8821 cgtatctctc cgttatgtaa gaccacaaaa cgatcacaat atttttctgc agtagctaaa
     8881 atatgcgtag acatcaaaat accggctcct tcttttttca tttctaccat caattcaagc
     8941 aatgcattga tagctagtgg atccaagcct aaaaatggtt cgtcgatgat atataaagaa
     9001 ggctcgatca aaaaggcgca taagatcatc actttctgtt tcattccctt tgaaaaatta
     9061 gctgggaacc agtcaagacg attagataac cgaaaagtct ccaacaactt ttctgctctt
     9121 ttcattgcta tatccatagg tatatcgtag gccatcgcag tgacttcgat atgttctctc
     9181 aatgtcaatt cttcatataa agaaggagtc tctggaatat acccaatttg tttgcgatat
     9241 tcttccgtat ttttctttaa ggatagatga ttgatttcta tcgttccttt tgaagcttcc
     9301 agcagaccga taatgtgctt gatggtcgta cttttccctg ctccgttcag tccaatcaac
     9361 cctactatct ctccaggctg tacatcgaaa ctgatatctt ttaacacagg gagatgtccg
     9421 tacccccctg tgacatgatc gataattaaa ctcattcttt ttcctccgcc atttattttt
     9481 atttattata ccacgaagaa aatgacggta ttttgttttt ttccttacaa tagctttaac
     9541 ttatggtaaa gttaagctga agtatttatt ctttatagaa aggatgtttt atatggaaaa
     9601 ctgtattttt tgcaaaatca tcaatcaaga aatcccaagc tataaaatat atgaagacga
     9661 caaggtctat gcttttttag acatctctca agcaacgaaa ggacacacat tagttgtgcc
     9721 taaacgtcat gtagcagata tttttgaata tgatcccgaa cttgcaggag aggttttttc
     9781 gcgtgtgcct aagattgctc aagcattgga aaaagctttt ccagaaatga aaggtcttaa
     9841 tatcttgaat aacaaccgtg aactagccta tcaatctgtt tttcattcac atatccattt
     9901 agtccctcgc tacagcaaag aggatgattt ttctattcac tttgtcaatc atcaagatag
     9961 ttatggatcg gaagaactaa aagctattca agaaacaatc gtaaagcagg tgtcctgcga
    10021 tgattaaaaa ctttttcaaa ggactgttat ttggagctgc tgctggcact gtcggaggtc
    10081 tgttattggc gcccaggagc gggaatgaaa caagaaagaa actgatcgaa gaattggaag
    10141 aagcaacaac tctgacaaat gatttaaata acagcctgaa tcattttaaa agcgcattga
    10201 tcgaaacaaa agaaacagct gaatctgtga ttcctgcttt ccaagaagca atcaaaaaag
    10261 atatcgaaga ttttaaattc caagccgaac cacgtatcac acaaatcaat gaacaagtag
    10321 aaaaactaaa agctgatctg ccagagtatc ctgaacaaat ataataatga ttattttctc
    10381 aacagattta actatataag tgagaaaagt ttgcgtattt cctaggaatg tgatgaaatt
    10441 ttgaattttg tttgaaacta ccacgacaac gtttttaaag gaaatgggat atggtatagt
    10501 taatatataa aaataataac taattacagg agtgcaaatt ttaatgaaaa aaaaatcaat
    10561 tattttggca gctactagtg cgttggctgt tttaactcta gcagcttgtt caggcgatac
    10621 taataaagat atcgcaacga tgaaaggcgg aacgatcact gtatctgatt tctatgatga
    10681 agcaaaactt gaatcatcga accaatcctt agtacaacgg atgatcattt ataaagtatt
    10741 caataacaaa tatggcgata aagtaaccga taagcaagtg gatgcagaat acgacaaaca
    10801 agcgaaatca ttaggtgata cttttgaatc acaattagaa gctgctggat atacgaaaga
    10861 tacttataaa gaatatattc gtaacaattt agcttttgaa gctggcttga aagctcacgt
    10921 agatattaca gatgacgatt tgaaaactgc ttggaaatct ttccatccag aagtagaagc
    10981 acaaatcatc aaattatcaa gtgaagatga agcaaaagat gtgaaaaaat cagcggatga
    11041 tggagatgat ttctctaaat tagccaaaga caaatcaact gatacagaga caaaagaaga
    11101 tggcggtaaa gttaaattcg actcaacgac aacaactatt cctgcagaag taaaagaagc
    11161 agctttcaaa ttgaaagacg gtgagatctc cgatgttatt actacaacaa atcctacttc
    11221 ttatgcgaca gaatactacg ttgtcaaaat ggtgaaaaac caaaataaag gcaatgacat
    11281 ggataaatac aaagatcaac taaaagatat cgctactgaa actaaactaa gtgacaatgc
    11341 attcacaact aaagtaatcg gtgaagaatt aaaagatgca aatgtaaaaa tcaaagatga
    11401 tgcctttgaa aatgtgttga gtgcattcac aacaacttca tcaagtacga aagactcaag
    11461 tgaaactaca gcttcaacaa agagttctga cacaaaatca actgattcaa caaaagaatc
    11521 ttcaactgaa gaaacaactg attcatcaaa ataaaagaaa acaaaaaaag cgaagactta
    11581 tgtctcgctt tttttgtttt gaatgaaaaa aataaatttt tgctataaat cttttggtac
    11641 atagaaactt cggttgtcca taccgaaaat ccgatcggtg tattttcctg gttctgtttg
    11701 acgaatcgaa gtcaacatca tctgcatcga agcatcgata ttgtcgattt gatgtaatat
    11761 ttctgcttcc attattcttg gacgtacagg tgagccatat tctaataagc catggtgtgc
    11821 taaaaccata tgacgtaata cgagaacatc ttcgtcagct tcgctgattt tcaacgcaag
    11881 acaggcttta gttatttctt cgtcaatcaa taccagatga ccgatcaaat ttcctgcaag
    11941 cgtatattct gtcgacatag gaccggaaag ttcaatcacc tttcctaaat catgcaaaat
    12001 gataccagca tacaacaatg ctcgattcag ctcaggatat tcatcggcga ttgatttccc
    12061 taagtgaagc attgtcaaag tatgataagc taatccactg gcaaatgcat gatggtttct
    12121 ttttgctgcc ggaaactcaa aaaattgttt ttgatattta ttaagtagat agcgaacaat
    12181 acgattccaa tgcgcattgg tgatctcaaa caacaattga ttgatttctt cttccatttc
    12241 ttctttcttc aaaggggctc tttccatgta taaactaggc tctcctggct catctaccct
    12301 tgttaagcgc atatgaagaa tctttacttg agggtttcct tgataaacct ctcttttacc
    12361 gtttagaaaa acgactgtgc cggctgtaaa tttattgatt tcctcctccg aagcatccca
    12421 gtatttacca tcgatagtac ctgaagtatc ttggaaagta aaagcaataa atttttttcc
    12481 gtttttagcg attcttacat ctgcactttt gatcaggaca aattgttcaa actgctcatc
    12541 gattactaaa tcacgaattt ttttcattat ttgctccttc taattgtttt acgatttggt
    12601 tcatctcatg gtaatagctt accatttctt tatcagaaga aaaacaaatt atttgatact
    12661 tttctccaaa tttagctaaa agttcagcta attgatactt tcgctgatga tcataatgaa
    12721 gccagccatc atcaatgatg atcgggcata tcgaacggtt agcttctaat gagaggtaag
    12781 caaatctcat cgccataatc agctgatctt tcgtacctgt agataactca tagatgggaa
    12841 attctgtata tccgtctgtt aaagtcagtg tatcattttt cagtaatatc ttctgataat
    12901 ttccttttgt taaaatacct aaataatcac ctgctgctct taacaactgg ggtaattgtc
    12961 tttcactcat ctctgttgaa agatcaccta agaatgtccc ggcgatttca agtgctcccc
    13021 attcctccag taattcctgt aattttgctt tttgccggct agcttgttga taaagttcgt
    13081 ccaatgtgcc gtctctttgc aactcttcta cttgaagttg gagtcgttgc cgattttcac
    13141 ttgcttcttt ttcttcttgc tggagttggg attgcttttc tttcagttgg cgcagtctta
    13201 cagataattt ctcttttgtc agctcttcag gaaatagtgg agtaagcatt tccgataatt
    13261 cattgtatct tttcatctgc tgttcggctt gtttggcttt ttgtaaaaag acaggtactt
    13321 ccgaaggata agataagcct gcttgagtca attgttcgct gtattcttgt atccatactt
    13381 tttgtttctt tttcaattga ttgatttctt gcttcaataa agtattttgc tgatagcttc
    13441 tggcaaattt catctgttcc atctcttgta taaaacggga aagcaatacc attttttctg
    13501 aaattgtctt atcttgtatc ggtaaccatt ctttaacaaa ctcaaatttt tggtcgattt
    13561 gtcgtagttt ttcttgtatt tcctgttctc ttttatgttt ccgctcaaat acgccatata
    13621 atttctgata atctatgact tcctgtccat attctttcat gagtttgagc gtatccatct
    13681 ttcctaagtg ccgatgatcc gcaaaacgat atacttcttg ctgaagaatc tgtttttctt
    13741 ctccagttct cttttgtttt tcttctgctt cagctaattg agctaaggct agatcaagtt
    13801 gtcctagttt ttcttgccac attcctttta tctcagagaa atcattttta gttttaattt
    13861 ttggttgcct tttgtagaag aaatagccaa tcatgcctgc accaattagt aaaaggatca
    13921 ctttcaaggg cattggtaca aatatacctg caatgattat tgctccgcca gccatcagca
    13981 tatacggttt agatgaagag actggtgcta tctctgactg attttgtaat aatgctggat
    14041 aggtctcttc caagtcattc acttctgctt caatcatctc ttgctgttct ttagctattt
    14101 ggagacgcac agaaatctgg ctttctagat cagcaatcct tctttcttca gccagaagat
    14161 gcttccattc ttcttcatat aaaaattctt ccggtaacgg atttttctgc caatgccacc
    14221 tttgttccaa aatcttcaat tctgaatttg tttctctagt ctcaatttgt aatctttgcc
    14281 attcttcgtt taattgttcc gctgtaaatt gaagttgctg gagatctttt agttcctgct
    14341 cctgctctaa ataaaaataa tatctggctg actgctgatc catcccgcta tgcttttcca
    14401 gtgttgcttc taattcttcc aaacgctcat ttgtttgctg ataccgtttg gagaaagact
    14461 ccagatctaa aagaagttgc tcatcaggaa gacctgtagt gctgtttttt ttgagagatt
    14521 gccattcttc atataaagaa aaattaagtt cttgctgtct ggctaatgaa aattgtttgt
    14581 tcgcctcttt taattgtgcc aataactgct gctgttgcga ctcgtattcc tgttcttgtt
    14641 ggatcaactt gccaaaataa gcttcctgtt cttgtttttg atgaattttt tcttgcagct
    14701 ctttccattc atttaatttt tgattgatta gtaatttttg gccttttttc ttgaataatt
    14761 gttgtgcttt ctgataatat tcttgccgct tttggaatac ttgagaactg cccgtgatgc
    14821 ctaaagaaat cagcgcatca tgtagctctt tttctctcaa atgatctagt tccatcaact
    14881 gctcttgctg gaaagtaaat acctgctgga ataactcttt cgtcaaaggt gcgatcagtt
    14941 tttctagtaa ctcatcactt cccacttgat cccccagcca caccttcgat tttccgcgat
    15001 tttgctgctt gtagcgctcg acagcaaatt caccatatac aggatgttta agccatattt
    15061 tgccgccata ggctgagccg tcttttggtg tataatcctt ttttttacgg ccttttgaag
    15121 ggaaaccaaa taatattgct tgaataaatt gataaatcgt tgattttcct gcttcgtttc
    15181 ccccatagat cagctgattg cctgaataaa agtcaaaact tttttgacgc cattttccaa
    15241 aagcagcaat ctcgatcctt aaaatcttca ttcctgatcc tcctcaaata aaaaatcttg
    15301 attaagcagc tgcttggctt tttcaagtgt ttcttcttga tattccggca gttcgctcaa
    15361 tattctagcc gcttcttgat gactatatac ttcatttaaa atttgttgga aatcttgagg
    15421 tgtctgatag tattgaaata actgttccat caatttagca gaagcagcaa gtggggtctt
    15481 cgtataaaaa gtattctcta tgagagaaat acgccaaata aagaaatcat ttatcctatc
    15541 tgagaattcc tctaacaaat agtctaatag ctctcctgat tgtatccgtt ccaaaacatc
    15601 tgtattgaga tggtcataat cttttaattt tatctcaatt aaactaaatc catcctctac
    15661 ttttgaaagc tgctgacgaa tgtgaacaat gacctctttt gtcgttcttg ctttcctcaa
    15721 tgatatttcc tttgtcttcc aatagacctc tgctacttta atcggatgta tttggcaact
    15781 tcctttactt aattcaacta gtagaataga tgtagaagct tgttcctttt tagtatgacc
    15841 ttgaggcgca cctggataaa caattgtttg cttttcattt aagaccgttg gcacatggat
    15901 atgccctaaa gcccaataat cgtatccctt ttcttgcatc tttgagggtt ggaaaggagc
    15961 atagtttcct ttttgagaga cgccaggttc cccgtggtac aacccaatat ggtaatctgt
    16021 gagttctcta gaaggaaatt ccatcacttt gtctttttga atccacggtt gacgataact
    16081 aaatccactg agagagacag tttctccact ttttatcgtt attttttttg tctctaccgt
    16141 ttcggatgta aataaatgga catttttagg aaaggagaac caataccgct ctttttgata
    16201 gaaatcatga tttccgaata ttataaatac aggtatatgg ttcttttcta accgtttcat
    16261 ctgttctgaa aaatgtttct gtatttttaa tgaaggacga ttttgatgaa aattatctcc
    16321 tgcaagtaaa acaaaatcaa cgttatttat tattgcctgt tcaatgatat ttgataaaac
    16381 ggtcagattc gtcttcaata gtttttcctg tacagtttta tcaagagttg tcagtccttc
    16441 aaatgaccga tccatatgta aatctgccgc atggataaat ttcaccatta aaaatttcct
    16501 cctttttttc ttttatcata ccattttcaa tgtttttttt catcaataac cgaaaatttg
    16561 ttcgtattat gcaaagaaat tacgataaat ggccgacttc aaaaaataag tttattcgaa
    16621 aaaaagattg tgacatgatt tttgtcacaa tcctaaatat ctatgttata ttcgcttcgt
    16681 ttgacactga agaagtacta tttgtactgg aatttatgct ccatttttcc gtatttagaa
    16741 ttctatgttc tatactacct taattagttt atatggtaag aattctttca aatgcttgag
    16801 atactccgtc tttatcattt gaaacagtga cataatcagc ttttttcatg atttcttgat
    16861 gactatttcc cattgcaaaa ctaatatctg cactttcaaa catagataag tcatttaaac
    16921 tatctccgat cgcgattctc aaaggtttct ttaaattgta gattgattcc aaacgatcaa
    16981 acaccacacc tttagataca ttttgaggta cgatttcaat atttacaggt gccgattcag
    17041 taacagacac atttagcatc tttttcaaat ggttaaatat attagctttc aattgacgat
    17101 taggaataaa aataaaaaat ttgtaaatgc tgatagcatc cttttctata atttcctcaa
    17161 tattattata agggattgaa caaatagatt tttcatactc aagc
//
LOCUS       DAXWLF010000044        16632 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXWLF010000044 DAXWLF010000000
VERSION     DAXWLF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.044"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(46..780)
                     /locus_tag="ACGE4U_001154"
     CDS             complement(46..780)
                     /locus_tag="ACGE4U_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFF8393331.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="ACGE4U_001155"
     CDS             1106..1516
                     /locus_tag="ACGE4U_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFF8393332.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="ACGE4U_001156"
     CDS             1509..2210
                     /locus_tag="ACGE4U_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFF8393333.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3823
                     /locus_tag="ACGE4U_001157"
     CDS             2210..3823
                     /locus_tag="ACGE4U_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8393334.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3813..4034
                     /locus_tag="ACGE4U_001158"
     CDS             3813..4034
                     /locus_tag="ACGE4U_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFF8393335.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4031..4792
                     /locus_tag="ACGE4U_001159"
     CDS             4031..4792
                     /locus_tag="ACGE4U_001159"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFF8393336.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5173..5682
                     /locus_tag="ACGE4U_001160"
     CDS             5173..5682
                     /locus_tag="ACGE4U_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFF8393337.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5752..6291)
                     /locus_tag="ACGE4U_001161"
     CDS             complement(5752..6291)
                     /locus_tag="ACGE4U_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFF8393338.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6431..7042)
                     /gene="rpsD"
                     /locus_tag="ACGE4U_001162"
     CDS             complement(6431..7042)
                     /gene="rpsD"
                     /locus_tag="ACGE4U_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFF8393339.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7304..8155
                     /locus_tag="ACGE4U_001163"
     CDS             7304..8155
                     /locus_tag="ACGE4U_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFF8393340.1"
                     /translation="MRGHVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPAATILVEGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLAYFASQGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8183..8818
                     /locus_tag="ACGE4U_001164"
     CDS             8183..8818
                     /locus_tag="ACGE4U_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFF8393341.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8838..9611
                     /locus_tag="ACGE4U_001165"
     CDS             8838..9611
                     /locus_tag="ACGE4U_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8393342.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10019..10816
                     /locus_tag="ACGE4U_001166"
     CDS             10019..10816
                     /locus_tag="ACGE4U_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFF8393343.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10794..11207
                     /locus_tag="ACGE4U_001167"
     CDS             10794..11207
                     /locus_tag="ACGE4U_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8393344.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11191..13836
                     /gene="mutS"
                     /locus_tag="ACGE4U_001168"
     CDS             11191..13836
                     /gene="mutS"
                     /locus_tag="ACGE4U_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFF8393345.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13854..15962
                     /gene="mutL"
                     /locus_tag="ACGE4U_001169"
     CDS             13854..15962
                     /gene="mutL"
                     /locus_tag="ACGE4U_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFF8393346.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15984..16544
                     /locus_tag="ACGE4U_001170"
     CDS             15984..16544
                     /locus_tag="ACGE4U_001170"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFF8393347.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5399 a         2971 c         3602 g         4660 t
ORIGIN      
        1 cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag ccataaataa
       61 taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa ctggtaccca
      121 tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt gtacaaaacg
      181 tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa gtgaagcaac
      241 taatgtccat acaaggaaac cgagtgctaa gtgagctgta ccaagattat cttggaagaa
      301 tggtgctttt gtaataccta aggcaccaaa gaataaaacg aatgaaccaa agaattcatt
      361 gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac ctaacacatg
      421 gttcacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga tcaattgtcc
      481 aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc aagggaatag
      541 accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc tgattccacc
      601 aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca gcatccagtc
      661 actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt tacctagaac
      721 gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg agtaatccat
      781 aaatacctcc aaaagttata aaaatgcaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaacca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagctatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agagggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc aggaagggta tctgtaaacg aaatggagga
     1501 aaatcaaatt gactgagttt tttgtgaatc cactatttgg cttaagtttg actttaggag
     1561 tgtatgtatt atctgataaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat cggtacagca aaagcaatcg
     2101 agattggcga aatagaagga atcatttccg gacttgcgat atgcgtaaat gggatattga
     2161 ccgttttgtt gctgccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatacatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaagtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg ggtcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgaccgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaaaaaac
     3781 tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt ttaccagaat
     3841 tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt tatgttgttc
     3901 tcgtatccga tgatgaattt gatggctgtc cagtgacacc ttacttattg atcaaaacaa
     3961 gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca gatgattggg
     4021 cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat ttatcacagg
     4081 agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag atgcttttgt
     4141 atttggatta gatcaaaagt tcggtaaaat ggaagaactc tctttgcgat ttcctgaaac
     4201 ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag cggtatcgaa
     4261 atgccaggat cgttttggtg aagttacgat tttactgaat acatcgggga tattggatgc
     4321 ctacaaacca ttgatggaaa ctgacgaatc gctgtgggat cttatttatg agacgaatgt
     4381 gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc gaaaaagcgg
     4441 aacaatcatc aacatggctt cgattgcggg tttggttgct ggcggaggtg ggatcgctta
     4501 tacaagtgcg aaacatgcaa tcgtcggatt taccaaacaa ctcgctctgg attacgcagg
     4561 acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa tgaatgcggc
     4621 tgattttgaa gggaaaggtg agatggctga gtgggtcgca aatgaaacac cagtcaagcg
     4681 atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc ctcaagcaag
     4741 ctacatacaa ggaacgatca tcccaatcga tggcggatgg ttgttaaaat aaccttgtgt
     4801 ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat gataaaagaa
     4861 agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat tctttttaat
     4921 aatcgttaat attcgaaact aagcggcttt tcacgacctt ttgatataaa cggtgttcaa
     4981 aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc tatttttatt
     5041 tgactattct tattacttgt tgcagggcag ctttttcact accttctgcg gcacttcagt
     5101 tggaattttc ttcccatcct ttggtcgcag tcattaccaa gataaaatta tcttgcaaag
     5161 gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac aacgacagat
     5221 gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt tttgtctgtc
     5281 tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct gtccttgtat
     5341 aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat ggcttctcct
     5401 cttggactga tagatgttgt agtaggaagc gtcagcacgc tgcttgtatt gcttatcaac
     5461 tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggctgtgac agcatgcgta
     5521 tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca actacctttt
     5581 ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt cggtgggata
     5641 attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt ttaatttaaa
     5701 atatggcagc gtggaagaga ctttgagtct tcttccatgc tgcttttttg attaatttgt
     5761 atgaagaaat cgatttagcc gttttttcat caagataccg atataagctg ctccagttgc
     5821 cttgattgct tccggcagta aaaacggcag tagtccactg gcaaatgcag ttgtaaaagt
     5881 aagatcacca cttattttca gccagatcgt tccgcagatc aatgcagcaa gagagcccat
     5941 tagattggca aagattgcat ataagtaatg agcatccgtt ctttcgatca tccaaccagt
     6001 tatccacgtt tgtaagagaa atccaatcaa atatccgcca gttggcccaa ataaaacagc
     6061 tatacctcca gtcatccctg aaaatacagg aagaccaatc aagccaagta aaagataaac
     6121 tacaacagat atcaatccta gttttcttcc gagaaaagtt gctgttaatc caatagcaaa
     6181 tgtttgcccc gtcaaaggaa ccaagccaaa aggaatggtc atctgagaaa ggacagaaat
     6241 aatcgcggca aattccgcag caagtattaa ttctttcaat gttcggttca taatgccctc
     6301 ttttcgttaa cttaatattt ttatttggtt aacgaaattg taaaacaaag tgattacttt
     6361 gtaaatagaa aatataaaaa aagaccagct atccaataga tagcggtctt tttcaagaaa
     6421 gcttttgaaa ttataagctt tggttgtagt attcaacgac aagagcttcg tcgatttctg
     6481 gatatagttc gtcacgttct ggtaaacgag tcaatgaacc ttccaatttt tcagcgtcaa
     6541 agcttacgaa tgctgggcgt ccaacagttg cttctacagc ttcttttact gttacgatgt
     6601 tttgtgattt ttcacgaact gagatcactt gacccacttc tacgtggtat gaagggatat
     6661 caacgcgttt gccatcaact aaaatgtgac catggttaac taattgacgt gcttgacgac
     6721 gagtcgttgc aagtcctaaa cggtaaacaa cattgtctaa acgttgttct agtaagatca
     6781 tgaagttaac accatgttta ccttctttga ttttgctagc ttttacgaac aagttacgga
     6841 attgacgttc gttcattccg tacatgtgac gaagtttttg tttttcagtt aattgcatgc
     6901 cgtattctga ttgtttgcca cggctgttag gtccatgttg acctggtgcg taagggcgac
     6961 gtgctaattc ttttcctgtg cctgacaatg agatgcctaa gcgacgagaa attttccatg
     7021 atggtcctgt ataacgagac attaaaattt cctccaatga atataagttt ttggagtaaa
     7081 ataatccgtt gaaaaattca taatcatata gtttgttctt caatcttcac ctttgcagcc
     7141 gcggttacac aatcgaaccc gagacggggc aacaaactag ggatgagtta ttatttttct
     7201 gctgcattat tttacacaaa ctacagtata tgatagatca tccacgaatg tcaagataat
     7261 tagtacataa aaacgcttta caaacctagg ctgtctttat aaaatgagag ggcatgtgca
     7321 aatggaaaaa agcgaaggaa gtttaaggag aaaatcaatg aatccggtat caccattatt
     7381 tgagaaaatc gatgcttcaa ttgaaaagaa ggcgaatctt tttgaaaaca gcttttcacg
     7441 ttacgctgtt cgagcggttc tcgcctgttt gtttttgact ttaggaacgg ctgtggcatt
     7501 tgcaatcgcg atgaaggggg aagatatcgc gcatggtcta ggaaagattt tatacgcatt
     7561 tatgttcagc tggggtctgg tcatgattct atacatgaac gctgaattag gaacatcgaa
     7621 tatgctttat atgaccgtgg gtgtttttcg gcagagactt gctttaagta aggcagccaa
     7681 gattctattt gcctgtattt ttttcaatat gattggcggt atattatgtg gatttcttat
     7741 ttctatgact acgccatttc aagatttaac gttggacaac tttatgttag aatcaatagc
     7801 agtaaaacta gcaaaacctg cagcaacgat tttggtggaa gggatgtttg cgaatatcgt
     7861 tgtcaatacc gctgtactga tcagcatgcg gatgaaagag gatgccggga aagtgatcac
     7921 agtcgtattc atcattttca tttttgcttt tttaggatat gaacacgtga tagcgaattt
     7981 tcctgccttt acgcttgctt actttgcttc tcaaggacag acggatggaa tgacagtcag
     8041 caatgtgtta cataatttgt tttttgcttt ggcaggaaat tatattggtg gcggtttagt
     8101 aatgggactg ggatatgctt ggctgaatca gtcaaaatca tcctatgtgg actaaaaagt
     8161 cgttcattaa aggggaagtg ccatggaagt tttagttttt ccaaaaaatg aggaaaaagc
     8221 gttacaaatg gaaaagtaca tgaaaaatct tttttccttt gctggagtac ctaagccgga
     8281 acgcgctctt ttacaacaac cgttactgga tgagagcaaa aatttatcta tttctgaagt
     8341 tttagctttt gtttattatt attatgaaca aatcgaaaga gagtatcagt atgtagcgat
     8401 cgatttagtc cagaaaaaca tcaaacggct tacatttgaa gacttagtcg tattgttgcc
     8461 actagttcag aaaaaagaat ggtgggacag catcgactct tggagaaaag tatacagcac
     8521 atggtgtaaa gcctacccag agcagcgtga agaagtcttt caattgtttg aaaaaaagga
     8581 agatttttgg ctacgtcgaa tagcaatcac gttgcaatta ggttataaac agcaaacaga
     8641 cacaggacta ttgatccgag caatagacga agatcgtcaa acaaaggaat tctttatcca
     8701 aaaagcaatt ggttgggcat tacgcgatta cagtaagacc gatgccgatt gggtaaaaag
     8761 acagctgaca acagatttga gtaagttagc tgttcgagag gcaagtaagt atttataaga
     8821 aagcgagacg acaagagatg agacgcaaat tatttgcttt tgatattgat ggaacgttgt
     8881 tagattcaaa caaacaagct ttagatagca cgagagaagc attggaaaaa ttacgcaaag
     8941 ccggtcatct ggtcacgatt gctacagggc ggagccgatt tcatgcgcaa gagatcattc
     9001 gcgatcttga ttttacgaac tacattttat gtaatggagc ggccggtttt ttagaccatg
     9061 aacaagtata taagaattta ctagatcgcg accagttgga tgcgttcgtt caagaagcgc
     9121 atcatgagca gatcgataca gcttttgtca gcttagacaa tatcaagcgt tcttcttcca
     9181 atgatgtaac ggtgatggaa gaagcgatgc attctttcgg tgctgtcttg cctgaattag
     9241 atcagttttt tgcagaggaa caagaaatat accaagcgtt agcatttttc gatcaaacat
     9301 atgatgggaa gttctcttca tatgataaaa ttcgatttgt ccgctggcat gagaacagtg
     9361 tagatgtcgt acctaaaaat ggttctaaag cagcgacaat tctgcactta gcagaacaag
     9421 taggcatccc tcatgaagac atcatcagct ttggcgatgg acaaaacgac cgtgaaatgt
     9481 tgcgcatgtc aggaattggg atagctatgg ggaatgctgt gccagaagta caagcagaag
     9541 ctaagatggt cactgataca aacgatcagg atggaatttg gaaggcattg aaagagttaa
     9601 gcgtgattta aaagaggttg tgaaaaagct gttctgcatc aagtaataag cccagccaaa
     9661 caaaaatagc tcctcctatt tttcgttttt ctgggcttat taccgcagat gcaagctttt
     9721 gaacaccgtt taaggaagag gtcgtgacag aagtggtcag tttcaagaaa taagaaggga
     9781 tttacgaaaa ttgcttctca aatttttgta aaattacggc ttatttctga agaagtagat
     9841 gaaacaacgt ttattcggga aatgggactg tgacaagatg tttgtcatag tcccattttt
     9901 ttattttatt tttcaaaact aagaaatacc aggaaagagt tccttttgtt tgaccttttc
     9961 tttcttattg aaaacaacta taatatagag tgatgttaaa agagaagagg aatttagtgt
    10021 gcgggtattg tttattggtg atgtagtagg ttcaatggga cgagagatga ttactgaata
    10081 tcttcctaga ttgaagaaga agtaccgtcc gcaagtaacg attgtaaatg gtgagaatgc
    10141 cgcttctgga cgtggaatca cagaaaaaat ctataaaaaa tttttgcaag acggcgtaga
    10201 tgtcgtgacg atggggaatc atacgtggga caaccgcgga atctttgagt ttgtaaatga
    10261 agctaaaaag atggttcgtc cggcaaattt tccggaaggg acgcctggac aagggatggt
    10321 ctttgtcaaa gtgaaccaag tcgaactggc agtgatcaac atgcaggctc gttcttttat
    10381 ggtggacttg gatgatcctt ttcgtaaaat gaatgagctg gtagaagaag ccagaaaacg
    10441 tacgccgatc atttttgtag attttcatgg agaaacaaca agtgaaaaac aagcaatggg
    10501 ctggttttta gatgggaaag tttcagcagt ggtcggtaca catacacacg ttcagacaaa
    10561 tgatgcgcga attttgccaa gaggaacagc gtatctgaca gacgttggga tgacaggtcc
    10621 ctatgatggt attttgggta tgcgcagaga acctgtaatc gaaaagtttc tgaccgcatt
    10681 accaaaacgt tttgaagttg tggaacaagg ccgatcgatt ttaagcggct gtatcttgga
    10741 attggatgat acgacaggtc atgcaaaaaa aatccagctg attcagatca acgatgatcg
    10801 accgttcatg gagtagaaag agaggcagtt ttttgcagca ggagccggaa attcaaaaag
    10861 aagtaaggaa actgacaaaa cttttaagag agaatgaaac gatcattcgc tacaaagaac
    10921 ttgaagaaaa gatccagcaa aaccagtatt tagctgagtt gagagaaaaa atcaaacaag
    10981 cgcaaaaaga tgctgttcat tttgcccact atgacaaacc agcagcagaa caagaagcaa
    11041 tcaaacaagc ggatcaattc atgcaagaat ttgatcagca tccgcttgtt gtagcttata
    11101 gaaaacagct gttggaagca gatgatctgc ttcatcattt aacaactatg atacaagaag
    11161 aaatcaatgg acaaatagag gaggaaaaac atgcctcaaa aaactaaaaa tactcctatg
    11221 atggaacaat atctaagcat caaggcccaa taccaagatg cttttttgtt ctatcgttta
    11281 ggagactttt atgaactatt ttacgaagat gcgatcaaag cgtcacagct tttggaatta
    11341 acgttgacga gcagaaatcg aaatgcagaa gaacctattc ctatgtgcgg tgtacctcac
    11401 catgcagcac aaggatatat cgatgccttg attgaaaaag gctataaagt agcgatttgc
    11461 gagcaggtag aagatccaaa gacgactaaa gggatggtca agcgcgaagt gatccagcta
    11521 gtcactcctg gtacagtcat ggacagcaaa ggattgtcgg caaaagacaa caatttttta
    11581 acagctgtag ttagtcaagg aaatgaattt ggctttgctt atgccgattt aagtacggga
    11641 gaattgaaga cggctcggtt gcatgatgaa gaagctgttt taaatgaagc ctctgcattg
    11701 cagacaaaag aactcgtatt aggcagtgag atcactgata cccttcgtga acagctaagc
    11761 gggcgtttag ggctagtctt ttctcaacaa aatgagatgg aagaaaacgc ggaatttagc
    11821 tttttgacaa gcgaactagt cgatccgctg aaaaaagaag caaccggcaa actattgact
    11881 tatttatcgg tgacacaaaa gcggagttta gctcatatcc aaaaagcagt agaataccaa
    11941 ccagatcatt ttttgaaaat ggattattat tcaaaattca acctagaatt aagccaatct
    12001 attcggactg ggaaaaagca cggcactctt ctatggttgt tagatgaaac aaaaacagca
    12061 atgggtggcc gtttactaaa acaatggcta gaccgtccgc ttatccaggc aaaacaaatt
    12121 aaagcaagac aagaaatggt cgcttctttg ttagacagct attttgaacg tatcgatttg
    12181 cagtcagcat taacgaaagt ctatgatctg gaacgcctag caggtcgagt agctttcggt
    12241 aatgtcaatg ggcgagactt gatccagctg aaaaattctt tagaacaagt gccaatgatc
    12301 agagggttga ttgaaggaat cgatcaaggt caatggcaaa gtctgctttc agaaatgcag
    12361 ccgatggacc atctcgtcca attgatcgac caagctatcc aagatgatcc gccgttacaa
    12421 ataacggaag gaaacatcat caaagatgga ttcaatgagc agctcgatat gtaccgttct
    12481 gctatgagaa acggaaagaa atggttggca gaacttgaag caaaagagcg tcaagaaaca
    12541 ggaatcaaaa acttaaaaat cggatttaac cgcgttttcg gttattatat agaaatcaca
    12601 aaagcaaatc ttggaaatgc agaactggag aagtatgaac gaaaacaaac tttagcaaat
    12661 gcagagcgtt tcatcacacc agaactaaaa gaattggaga cacaaatcct cgaagcagaa
    12721 gaaaaatctg ttgatttaga atatcagttg tttttagctg tgagagaaga agtcaaaaag
    12781 gctatccagc cgctacaagt acttgcaaaa gcaatcagtg cagtagatgt gctacaaagt
    12841 tttgcaacga tcagcgaacg ctatcagtat gttcggccag aacttgtcag tgacaagcat
    12901 cagcttctga tcaaagacgg acgacatcct gtagtagaaa aagtattagg acatcaggaa
    12961 tatatcccta acagcgtaga aatggcagaa gacgaaatga tcttgctgat tacaggaccg
    13021 aatatgtctg gtaaaagtac gtatatgcga cagttggcat taactgttct gatggcgcaa
    13081 atgggctgtt tcgttccggc aaaacaagca gttttaccta tttttgatcg gatttttacc
    13141 cgaattggtg caagtgacga cttaattgct ggacaaagta cttttatggt agagatgatg
    13201 gaagccaatc aagccctgcg ttatgctacg ccaaacagtt tgattttgtt tgacgaacta
    13261 gggcgaggaa cagcaaccta tgacggaatg gcgttggccc aagcaatcat cgaatatatc
    13321 catcgtcatg tacaagcaaa aacactcttt tctactcatt atcatgaact gacggtactt
    13381 gagaaagaac ttccgcagct taaaaatgtc catgttggag ccgttgaaaa agacggagaa
    13441 gttgtcttct tgcataaaat gatggatgga cctgcggata aaagctacgg catacatgtg
    13501 gcaaagatcg caggattgcc ttccaatcta ctagaacgag cagcagatat cctgcatacg
    13561 ctagaaaatg gcgataacgg acaccatact gttccagctg aagtagaaga gaaaactaaa
    13621 acagccaaac aggagcaaat aaaaccacct gcagaaaaag taaatcaaga ccatatacaa
    13681 gaccgtgtaa aagaagaaaa ccaacagctt tctttattca gtgaattatc cgaaaatgaa
    13741 acagaagtca ttgaatcact gaagcaaata aatttactag aaatgacgcc aatggatgca
    13801 ctaaacacat tatatgaatt acaaaaacaa ctgtaggaag aaggcggata tcgatggcca
    13861 aaatccaaga attatctgaa cgtttagcta atcagatcgc tgcgggagaa gtagtggagc
    13921 gaccagcttc cgttgtgaaa gaattagtgg aaaatgcaat tgacgccggc agtactcaga
    13981 ttgatatctt agttgaagaa gcgggactgc ggacgatcca agtgattgat aacggagaag
    14041 gcattttggc agacgacgta gagaatgcct tcaagcggca tgcgacaagt aagatccata
    14101 atcgcgacga tctttttcgc atacgaacgc tgggctttcg aggggaagct ttgccaagta
    14161 ttgcctctgt ttctgaaatg actctggaaa cggcgaatca ggaagaaaaa caaggcacct
    14221 atctgtcgct gaaaggcgga gaagttattg agcatcgacc agcaccactg cgtcaaggaa
    14281 cgaagattac cgtcagcaat ctgtttttca atacgccagc ccgcttgaaa tacgtgaaaa
    14341 cactgcacac agaattagcg aacatcgggg acatcgtcaa tcgactggca ttgagtcatc
    14401 catcgattgc ttttcgttta gtacatgatg gaaacaaaat gctggcaact gctggaaatg
    14461 gcgatctgaa acaaacaatt gcgggaatct acggattatc taccgcaaaa aaaatgctga
    14521 aaatcgaagg aaaagatcta gatttcgagt tatttggcta tgtttcattg cctgaggtga
    14581 ctcgtgccag taggaattac ttatcgacga tcatcaatgg acgttttatc aagaactttt
    14641 ccttgaataa agcaatcgtt gcaggttatg ggtcaaaatt aatggtcggt cgtttcccta
    14701 ttgctgtatt ggaaatcaaa atggacccgc ttttagtaga tgtaaatgtt catccgacga
    14761 agcaggaagt ccggctgtcg aaggaaaaag aactaacaga gctaattagt aacgccattc
    14821 aaaaagcatt gtctcaagaa aatctgattc caagtgcagc ggataatcta cggtttaaga
    14881 aaaaagtacc tgatcagcct aaaacagaac aaatcgaaat cgatttgtcc tacgcttttg
    14941 aagaagaaaa acctaaaaag ccaagtagtt tgaattttga tcgagagaca ggtacctttt
    15001 tcgtagaaga accaaagaac acatttgttg acagaattgt tgataagtct gtggaaaatg
    15061 ttgaaaacta ttctggtttt tctgaagaaa acaccactta tccagaaaaa acagcgaaaa
    15121 cggtggataa gtttggtgaa aatgttgata actttgtaga aaaaacagtg gaaaaatctg
    15181 aaaagaaaga atttcccgtt gtggataaca tggagaaaac aaatgaagaa caagcagata
    15241 aatccgtaga agaggatgaa aagatttacg aggaagaatt gacactccat cctgaatttg
    15301 atttcactca aggaaacgac aaaacattag aacaagccat caagaaatta gaaacggaaa
    15361 aaccagtaca acgttttcct cagttggaat attttggaca gatgcacgga acgtatcttt
    15421 ttgcccaaag tagcgatggg ttgtacatca ttgatcagca tgctgcccaa gagcgcatta
    15481 agtatgaata tttccgggaa aaaattgggg aagtatctga tgatctgcaa gaattgcttg
    15541 ttccatttgt gttagactat ccaaacagtg atgctttgaa attaaaagag caaaaacact
    15601 tgctagaaga tgcagggatt tatttagaag aatttggtca aaacagcttt attgtacggg
    15661 cccatcctac ttggtatccc aatggagaag aagaatcgat tattcgggag atgatcgata
    15721 tgctgctgac aactggaagt gtgagtgtaa aaaaattccg tgaagcaaca gcaatcatga
    15781 tgagttgcaa acgatcgatt aaagccaacc attatttgaa tgaagcacaa gcacgtgtgc
    15841 tgctgaagga tctggcacga tgcgaaaatc ctttcaattg tccccatggc cgtccagtat
    15901 tgattcactt taccaattcg gatatggaaa ggatgttcaa acgaatccaa gacccgcatt
    15961 aagtcaaaga gaagagggaa aacatgaaga ttgtattagc atcccaatca ccaagaagaa
    16021 aagagttatt ggctcatatc gtttcagaat tcgagattga acctgctgat atcgatgaaa
    16081 cgccatttcc agacgaacag cccgtcgatt atgttcgtag aatggctgaa gaaaaagcac
    16141 gaattgtctg ggaacaagat gaaaaagaag accatttagt gattgccagt gacacgactg
    16201 ttgttctcaa tcaagagatc atgggtaaac cagaagatct ggctgaggct gaattcatgc
    16261 tgaaaaaact aagcggggaa acacatactg tatatacagc tgttgtttta aaaacaaagg
    16321 aacgagaaga acggatacta gcagaagcac atgtcacttt ttatcctttg acagacgaag
    16381 agatcaaacg ctacttggag acgggggatt atgcggataa ggcaggggca tacggcatcc
    16441 aaaatgctgc gagtgtattc gtcaaagaaa tttctggaga ttattacagt atcgtaggat
    16501 ttccgattgg tgctgtcaat caggcactga aaacattcat ttaaacaaaa aagcggacta
    16561 tctgattttc gattgaaaat ccatgatagc ccgttttttt tattgaaaag attacttatc
    16621 tactctgact aa
//
LOCUS       DAXWLF010000045        16512 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXWLF010000045 DAXWLF010000000
VERSION     DAXWLF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.045"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..546
                     /locus_tag="ACGE4U_001171"
     CDS             <1..546
                     /locus_tag="ACGE4U_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8393348.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="ACGE4U_001172"
     CDS             735..1403
                     /locus_tag="ACGE4U_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFF8393349.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1989)
                     /locus_tag="ACGE4U_001173"
     CDS             complement(1603..1989)
                     /locus_tag="ACGE4U_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393350.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1961..3343)
                     /locus_tag="ACGE4U_001174"
     CDS             complement(1961..3343)
                     /locus_tag="ACGE4U_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFF8393351.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3499..4686)
                     /locus_tag="ACGE4U_001175"
     CDS             complement(3499..4686)
                     /locus_tag="ACGE4U_001175"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFF8393352.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4693..5724)
                     /locus_tag="ACGE4U_001176"
     CDS             complement(4693..5724)
                     /locus_tag="ACGE4U_001176"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8393353.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5740..7194)
                     /locus_tag="ACGE4U_001177"
     CDS             complement(5740..7194)
                     /locus_tag="ACGE4U_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFF8393354.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            7369..8274
                     /locus_tag="ACGE4U_001178"
     CDS             7369..8274
                     /locus_tag="ACGE4U_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFF8393355.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8365..9042
                     /locus_tag="ACGE4U_001179"
     CDS             8365..9042
                     /locus_tag="ACGE4U_001179"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8393356.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9039..9209
                     /locus_tag="ACGE4U_001180"
     CDS             9039..9209
                     /locus_tag="ACGE4U_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393357.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9323..10219
                     /locus_tag="ACGE4U_001181"
     CDS             9323..10219
                     /locus_tag="ACGE4U_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8393358.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10274..11644)
                     /locus_tag="ACGE4U_001182"
     CDS             complement(10274..11644)
                     /locus_tag="ACGE4U_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8393359.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11882..12628
                     /locus_tag="ACGE4U_001183"
     CDS             11882..12628
                     /locus_tag="ACGE4U_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8393360.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12625..13464
                     /locus_tag="ACGE4U_001184"
     CDS             12625..13464
                     /locus_tag="ACGE4U_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8393361.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13465..14415
                     /locus_tag="ACGE4U_001185"
     CDS             13465..14415
                     /locus_tag="ACGE4U_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFF8393362.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14556..15308
                     /locus_tag="ACGE4U_001186"
     CDS             14556..15308
                     /locus_tag="ACGE4U_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393363.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15327..16478
                     /gene="queG"
                     /locus_tag="ACGE4U_001187"
     CDS             15327..16478
                     /gene="queG"
                     /locus_tag="ACGE4U_001187"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFF8393364.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5371 a         3088 c         3317 g         4736 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttttcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt tgataccgtt gactagttct tccatcgcaa aagcagtccc
     1921 ccattttaat ccaaatggcc gatctcgata ctcgttatag tcaatgaatt tttttgctgt
     1981 ccgtctcatc ttttccacct tctaatccac tcataccacc tgcatgcccg ccaacgagag
     2041 aactacgtgc gatggcgcgt cctccttgca agagactatg cgcatggatg attgattgaa
     2101 agccgtattt ttttctaatc gtatcgacaa caaaatcaac tttcgcttca tttatctgct
     2161 tttcaggatc ttcaaaaagg ttaagctgta aacctgtcgc gtatacgagg tcggaacagt
     2221 tcacgcctac acgccgaata tcctggtagt ggtaatgctg gtggaaaatg gtcaataaag
     2281 catctgctag atctctgcta gcatttgtcg cttcgatttt catttgctgg tgaaacccgg
     2341 tcttgccatt tggatcaaga tagcctaaag agtatcctac ccataagctg actacttgtg
     2401 tttttgctgc agatcgtctc aagcgagtgc cgacttggtc ggccatttct ttgataacga
     2461 tctcgatttc ttctcgtctt gtataatctc tatctaggat ttgactattt ccgatacttt
     2521 ttgattttgg tgtatattct tgtcccaaaa aacttcgatc gatgccccaa gcatgggcat
     2581 aaagctgagt accgatgacc cctagcttgc ttttcaagtg ataataatcc gcgtgagcta
     2641 aatcataaac agaatgtata cccatttgtt tcagacgttt tgctgttcgt ttgccgattc
     2701 cccaaaagtc agtgatatct gagattttcc ataacttttc tggtacatct tcataacgcc
     2761 attcagcaaa catttgcttt ccgtcttttg agccattgtc taaggctagt ttagcaagta
     2821 gcgggttatc gccaattcct atagtcgtgt agattccggt ctgctcaaac acatctttat
     2881 ggatcagttt agccatgtcg tacgcatttg ttttaccaaa gagtgccatg ctatttgtta
     2941 catcaaggaa gctttcatcg acggaataga catgatggtt cgcttcgtct gcatattttt
     3001 tatagataga attgatttct ctgttctttt ccatataata ggccatcctt ggggcaacaa
     3061 tatataaatc acttggatag gggaagggaa gatctctggc acggctgata ttggtgatgc
     3121 cgtaagcttt ttttgctgca ggactggaag ccaagattaa tccgctaccc cgtgtttctg
     3181 tcgaatcgga cggataggac atgactacta atttagttgt aaggggatca agatcctttt
     3241 caacgcactc tacggaggcg taaaaagatt tgcagtcaat acataaaata tctcttgaag
     3301 gttctttttc ataaacaaat accggatttt gattcgccca cataacaatc acctcttcaa
     3361 cacaattaga acacacgttc tctttccgtg tcaataaaaa acagcaattt tcaaatacga
     3421 gcaaaaggct cacatttgga aattgctgag aggttatttt tatctatttg tttttagctg
     3481 agatcgttga tttttttatc aaacatgaac agcttctgga tcgatctttg ttgtcccatt
     3541 tccaatgtgt gcaacataaa attccggagc atagccgaca actttcatgt attcatcata
     3601 gacattgttt ttgaaggcag gtatattttc ttctttgaca agagcaatcg cacagccacc
     3661 aaatccagct cctgtcattc gtgcacctaa gacaccttct tgtttttgag cagcatctac
     3721 tagtgtatct aattcgatcc cagttacttc ataatcatag cgaagagagt ggtgagaatc
     3781 atttaacagt tgaccaaatg cttctaagtt cccagcttct aattcagctt ttgccttcaa
     3841 agtccgctga ttttcagaaa cggcatggcg agctctgcgt atcaatgtat catcgccgat
     3901 ttggtcaata gttgattcaa attcttcttc atctaagtct cctaaagaag agataggcaa
     3961 agttttttgc aaacgtgcca aggcttcttc gcattcgcta cggcgttcat tgtattttga
     4021 atctgccaat tcccgccgct tattcgtatt catgatgacg attgcataac catctagttt
     4081 cactggaacc atttcgtatt tcagcgtatt tgtatctaat aagattgcat gatctagctc
     4141 accgaagccg atggcaaact gatccatgat acctgaattg acaccgatga attcattttc
     4201 tactttttta cctgtttgga ctagttccaa gcgatctgtt tttaaatcaa acagatcttc
     4261 taatacaaca cctactaata attctaatga agcgctactg gaaagtcctg caccattagg
     4321 gattgttcct tccactaata actcaaaccc atgatcgatt gagtgtccag cttcttttaa
     4381 tttaaggacc attcctttta cgtaatttgc ccagtttgct tttttatcat attctagctc
     4441 atctaaagta aaactaatga caccgatatc gtcaaagttt gtggaataaa ctaatatttt
     4501 attatcgtct cgaggtgctg ctacaccttg cgttccataa gtgatggaag cggggaagac
     4561 atggcctcca ttgtaatctg tgtgttcacc aatcaaattg atgcgtcctg gtgagaagta
     4621 ttgtgtagtt tttttaggtc cgaatgtttc ggcaaatttt gtgtttaagc ttcttttgat
     4681 ttccatgatg agttattcct ttcttgtttt gattttgaat tctgtcactg tttcaaatac
     4741 tgatccggct tttaaatgaa tcgaaccaaa tgctgagaag cgctctgcac ctggagctgt
     4801 ttgagtctca aatgtgatgc caccgtgatg tgccagtttt cggttgcgca tttctggtgt
     4861 ttctgtgccg aaatttgcag tgaaaataac cacactagaa gcatctgtag caatatctac
     4921 ttggattctt ttatcggggc tagtcaagcg agcggcttct ttacccaagc cggtttcttt
     4981 taagaaaaat ggatgatcga ttccgtcaac aagatttttt tgatccaaat cactagcaaa
     5041 aacggacgct aattttttag gtatttgaaa atcgaaagca cttcctgcag cattttcttt
     5101 tacccctata ggaatactat ctgttcgcaa aggagcataa gcttcgctat tgagccaaag
     5161 ctcatgttga tcgattgact gagaagcatc tcccgtcaag ttgaagtaaa catgatttgt
     5221 cggattgaat aaagtatctt ggtcactggt gccgcgagag gtcactcgcc agatattatc
     5281 ttttgtcaaa gtatagcgga tttccacatc cattgtacct ggaaaaccat gttctccgtc
     5341 gggacttgta gtagtaaaaa tgacagaagc ttcattttcg ccattcaaaa tgacataaga
     5401 ccattttttt aattcaaatc caggagcacc accatgcaga ttatgtccag tttttggatc
     5461 tgtggctaat tggtaagttt ttccattgag agaaaagtgg ccattttcga ttctccctgc
     5521 ggtgcgaccg atagaagcac cgatatacgg atctttttcg atatattctt cagcggtgtc
     5581 aaatcccaaa actaattctc gatcatttga ttttaaactg acgattctag cacctaaatc
     5641 agagatagaa agtgctagct tgtttttgtt ctccaaggtt atcaagtgat aaccttggcc
     5701 aaaatctttt tctgtaatct tcattttcaa gatcctttct taatgggaag gaactactgc
     5761 atcgtgaagt tcatcttcca ttgtatcagc tggagccaat ttgttttcca atttctcaac
     5821 gatttgtgca tgttttttct ctgtcaatgt aatcttccaa gcaaaaataa tagcggagaa
     5881 aatcatcaaa gcagcaggag catagaatgc ataagctttg aaagtcgtta cgccatgagc
     5941 agtgatatca gcagcagtag cattaccagt catcccagaa gcgatagcga caaaccctac
     6001 gatcccgtta gacaaagcac cagcgatttt atctagtaaa gggcgaattg atagagtaac
     6061 agcttcgtta cggataccat ttttccattg accatattca acagagtctg tgatcgtcat
     6121 caaagcggat aagaaaatca attgttgagg gaagtagaac aatactagcc cgatgataac
     6181 gattggcaaa cttgaacctg cgatcgtaaa gaaaaagtaa gaagaaatca tcattgcgat
     6241 ccctccaagg taaacgtagc gacgagagat gacgcgtgta agcattggga atagtggaac
     6301 agctactaaa ccaagtaagg cagcaactag accaacgatc caaaattcag ttggttggcc
     6361 aagtacatat ttgaagaagt agaataatac ggcagttgtt gctacattgg caatcgcata
     6421 agcgatataa gcaagggcaa gccacatcaa ctgatcattt tgagcaactg ctttgaacac
     6481 atctttgatc ccagcttttt cattttgctg acggatcgca gtatcgactt cttttgtgcc
     6541 caaggcagtg ataatcgcag taccgcctga aataacagca gcaatcacac cgaaccagaa
     6601 ccagcctgaa gcgccttcaa tatgtttatg agtaacaaga taggtaaaga aagtaacgat
     6661 agggacaacg actagagtag taccatttgc acctagagaa gagccgaaac gagcaaacgt
     6721 agcaaatttt tcacgttcct ttgtttccgt actcaaagct ggaatcattg accaaaaagc
     6781 gatatcctta aatgaataaa aactatccaa taagatgaaa acgattgcaa ataatatgat
     6841 aaataaagtc gtattatgag tagctaagcc aaagaaattg gtaaagattg ctaccaagag
     6901 tacggaacta acaacgccgc cgataactaa ccatggtttg aatttaccgt attttgtacg
     6961 agtattatcg atgatgccac caatcaaagg atcaaaaata atttcaacta agcggatacc
     7021 tacaactaag cttgtaactg tagcaatcat ttttgcattc gttgccttat ctgttcctgc
     7081 gaacatctgg cttgtcacga ataccataaa ataagtgctt aatgtaacat agaatgtgtc
     7141 gtgtccaaaa gcgcctaaag cgtaggacac acgttggatc attttgcttt tcataatctg
     7201 tattcctctc ctgttcaata tataatacta agtaaaccgg ttacaacgaa taacaaagat
     7261 cttttaatga cttaaactta acaagaaatc gcttcctttt taatatattt ctgtgacttt
     7321 tatttaactt tttgttgcat tatactaaag gagtaaggag ggatagacgt ggagcaatcg
     7381 atttttttaa aaaaggaaca tcaacaagta gaaaaagaat tgtattttga tttttgtggt
     7441 ttctctaaaa cgatgccgtt tcattctttt ggtcctgcta ttcgcaatga ttacatcctg
     7501 catgtcgtga tggaaggcaa aggatcttat catgtgaaaa atcaacaatt ccaattgaaa
     7561 aaaggtgatc tgttcttgat tcgtccagga gactcgacgt tttacttatc agatggagaa
     7621 gatccgtgga tgtattgctg gctttcattt ggtgggagtg ctgcagaaga aatcattcgg
     7681 cagtcgcttt tcagagaaga ccaatacacg atggtctctt ctgacataca aaaatatatc
     7741 gacattattt tagaatgcat gaaagatacg gaagaaacct tggaaaatga attacagtta
     7801 acagcattaa cctaccgttt tttatcagtc cttttaaaag atggcggaaa ggtcagcggt
     7861 ggtgtacaaa aggttcattc ctctttagct gttgaaacag tcaaatacat cgaggcacat
     7921 tatgctgaaa aattgtctgt tgaggaaatc gcccagcatt tagcggtcaa tcggagccat
     7981 ttgtcgcgag tattcaaaaa tcatatgggt accagtatca aggaatattt gatcggggta
     8041 cggatcaatc gaggggcgtt tcttctttct ctgacggatg aatccgtgga aagtattgcc
     8101 tatcaagtag ggttcaatag tctagtcgta tttagccgaa tgttcaaaaa gagtaccgga
     8161 gaaacggcta cgaactatcg taaacgtatg aaaaacgaaa attttaagaa tattgctttg
     8221 gataccttga aaaaacagtt ggaaaaacaa accgcagttt cttggacaac ctgattagtc
     8281 tggttaaaat gacaagcatc ggcaaaagaa acttgtggcg gttatgctac actgtaaagc
     8341 gaagactaaa aaggaggaaa agggatgcgt acacttatca tcattagcca tccaacgatc
     8401 gaagaatctt taaatcagca atttttcaaa gaagcttctg ctggtttacc agctacttgg
     8461 catcatttgg aatcaagtta tccagacgga aaaatagata tagagactga actacagctc
     8521 ttggcccagc atgatcggat cattttccag tttccgtttt actggtatag ttctcctgct
     8581 catttaaaaa tttggcaaga taatgtcttg gaacgagcag cacatgtatt aaaagggaaa
     8641 gaactaggaa tcgtattgac aacaggtgtg gcggaaaaag aataccaagc tggaggaaaa
     8701 gaagaatata cgatttccga atttttgagg ccgtatcagc gaatcgcaaa taaattccat
     8761 atgacctatc ttccgccatt cgttttagca caatttatgt atttatccca ggaaaaacgt
     8821 tgggagaagc taattgctta tcagcagtat ctctcattgg aaggcaaacc atcgctgact
     8881 cagcggattg actggtttat tcaaagagta caggaaaatc agaaaatgca ggaagaagaa
     8941 tctgaaaaac aagcatatat cgtagaagca ctgactgagg caaaagagca gatcgaagac
     9001 ttgaccttta cactgcaaga aatgaaagga acttcattat gacaaatgat tggctgatcc
     9061 aacaattaga tgaattaagc gaaaaatcag aatatcaaga ccgtgcgctc ttatatgaaa
     9121 taaagaaact gatggctgag atagacgaac gaacaatgca gctgcaagga gaaatcgacg
     9181 gacaatcttg gaatcatctc aactggtgaa attttcttat aaataacaaa gataaaaatg
     9241 tgcacagcga caattatttg ctacaataag aatatgaagc gtttgataac gctattatac
     9301 ataaaaattg gtggtgaaaa aaatgtatga tgaaaatgag tggggagaaa atgttcggat
     9361 gacccgaaga agacagcaaa aacaagcagc tcgtaagcgg atgatcatga ttggggcagc
     9421 agttgccgtg tttttgatca tagcaggagg agttgcgggt ctatattttc atggaaaaaa
     9481 ttcgtcagaa gcgaaaagta cgaccataac ggaaacaacg acaaagaaaa cagagacgaa
     9541 acaaacaagt gagtcatcaa ctaaaacaaa agagacacaa acttctgaag aaaaaacagc
     9601 aaatggtttg ccagagcaac ttcaagcaat cgtaaatgat tattcgaaaa agatcgaaga
     9661 aaagacgcct gcattgattg aagaatacca gacagaaatt cagggaaatc aagagggaat
     9721 agctggttta tcagcagttg ctaaccaaaa agcacgtgag cttcaagcaa tctccgacga
     9781 agggatcaga aaattacgtg cagcgtatca ggcagcagaa aataaagacg gtgtagactt
     9841 agatactttg atcaatcaat tatcggctaa ttatacgaat catgtcgcta agatttccga
     9901 tatttatttg cagactagcg cctctcttca agcagagtca acaagcacac aagacacaac
     9961 atcttctgat tctgagacag cagagtcggt agaagcttct gaaatgcaag ctcgaacaac
    10021 acaagacaca acagattcct ctgaaactaa taccaacagc gcgacttcac aggctgcgac
    10081 aaccgtagtt ttggaaggag aaggtccaaa tcaaatagcc gaacgaactg gcgtaccagt
    10141 agaaacgatc ttgtcgctga atgggatgag catggagaat tatttcctga ctcccggtga
    10201 gacgctaaaa ttgaattgat aaaagcaata aaaaagaacg aagcttccac taaggtgaag
    10261 cttcgttctt tttttatttt ccggttcttt tttcgcgtac aaagtaatgg atcgtcatgt
    10321 aaagcaagaa aacgagtcca ataatcaatg agattctcgt ttcaggattg ataaacatga
    10381 aaatcaatac gatagctaaa aaagcaagtg tcacataatt cgtataagga gatagtggca
    10441 ttttaaaagg atgatcatgc attttatctg cgtgcttctt tttgaagcga atctcgctga
    10501 tcaaaatcac tacccaagga accataccag gcaaaatact tgagctgtat acgaaaacga
    10561 aaatattttc tgactggttg atgaacattg gtagaagata atttaagatc agaccaatta
    10621 agattccacc tgagattgct acgacaggat aaaaaggcgc accgtgacga gatagtttca
    10681 gaaatttttt agacatcttt ccatttatgg caagtgtata gaccatacga cttgcgctga
    10741 aaatccctga attgcaacct gataatgccg ctgtaatcac gacaaaatta atcagtcctg
    10801 cagcaaatgt aatgccgact ttagaaaaag tttgtacaaa aggtgaacca atctcactca
    10861 attgattcca cggatagata ctcacaatga taaaaatagc accaatataa aaaatcaaga
    10921 ttcgaccgat agtggactgg acagcttcaa tgatcgtctt tttaggattc tctgcttcac
    10981 cagcagtcat tccgatcagt tcgattcctt gataggaagc aacaacgatc gataatgcaa
    11041 agaaaaatcc tttgacgcct ccagtgaaga atccaccatt tttccaaaga tttgaaatcc
    11101 ctactgcctg accatgatta ccaaatccaa ataagattac cccaagacct gcaatgatca
    11161 tcaaaatgat cgttatgact ttgatgatcg aaaaccaaaa ttctaattcg ccaaacgcct
    11221 tgacagaaat aagatttgcc agcataagaa acaagacgac aatgattccg ggaataattg
    11281 ccggcaaatc aggccaccaa taattcatat aagatcctac agcaatcact tcacttattc
    11341 ctacgacaat atattgaaaa atattactcc aagcggtcaa gtagccagcc acaggatgga
    11401 tgtactcgct cgcgtaattt gcaaaagaac cagtcgaagg atcaacgtaa agcatttcgc
    11461 ctaaagcacg cataatcaaa tacaaaaaga atcctgcaat cgcataggct cccatcactg
    11521 agggccctgt ccaacgtatg gttgaggagg cacccataaa cagaccgaca ccgatcgttc
    11581 cacctagtgc gatcatttgg atatgacggg cactaagact tcgagttaac ttttcttctt
    11641 ccatacaaaa actcctttcg ttgttgagtc aacaagaaaa aataatcaaa cgtttcaaca
    11701 catgtttttc attattaggt tagccttatt gaactcttga tgtgattcta cagaaaattt
    11761 tcataaaatg cgaggaaaat tcgaaaaaaa ctttttcatc attctacttg aaaatgcttt
    11821 ccattttagt taaactatat ttaaataaaa agttaggtaa acctaaaaag gagtgtaaga
    11881 tttggaacgt acggccattg taattcaaga cctttcagcc gcttatcaag ggaaaactgt
    11941 tttgaaaact atcaacttga cgataaatcc acaaaaaatt accggtatta tcggaccaaa
    12001 cggtgcagga aaatcaacat ttatgaaatc tttgctagag ttgataccag ctactactgg
    12061 taaagtcacc ttttctggac agtcggtaaa ctcagtccgt aaaaaaatag cctacgtgga
    12121 acagcgaagt gaattggatc tttcctttcc aattgatgta ttaggcgttg tacttttagg
    12181 aacatatcca tctttacgaa ttggacaaag acctgggaaa aaagaaaaag aacgtgcaag
    12241 acaagctttg aaaaaagtag ggatggaaga atatgccaaa agacagatca gcgaactatc
    12301 gggtggacag cttcagagag tttttattgc aagagctcta gcccaaggag cagaatggat
    12361 ctttttagat gaaccattcg tagggattga tgcgttaagt gaacgaaaga tctttgacat
    12421 cttgcaggaa ttgaagaatt caggaaaaac gattttgatc gtccatcatg atcttcataa
    12481 agtagaagaa tatttcgatg aggttattct tttaaataaa cagctgatcg cttccggtcc
    12541 agtacaagaa gtctttacat cgaagaacct tcaattggcc tatggtgaga tcatcagaca
    12601 tttagtaaaa ggaggagaga aaaaatgatc caatccttta ttgatggatt aatgaattat
    12661 caattcctgc aatacgcttt ggtcacttcc atgcttgtag gacttgcttc tggagtcatc
    12721 ggttctttta ttattttacg agggatgtct ttgatgggag acgctatctc tcacgcagtt
    12781 cttccaggtg tagccatttc ttatatgttt ggttttagtt acatctttgg agccactgct
    12841 tttgggatgc taacagcagc agtgattggt ttcgtgactc agcggagccg attgaaaaat
    12901 gatacagcga ttggaattgt ctttagctcc ttttttgcat tagggattat cttgatttca
    12961 tatgcccaga gtgcgacaga tttgtatcat attttatttg ggaatgtctt agctgttagg
    13021 gaaagcgatt tgttgctgac agcactcgtt agtggaatcg tcttgatatt tgtctttttc
    13081 ttttataaag aattgaagat cacatcattc gatccgacaa tggcaaaagc ttatgggctg
    13141 aacacatcgc tgatccatta tcttttgatg ttctttttga cattagtggc tgtagtcagt
    13201 ttacagacag taggaacaat cttggtgatt gccatgttga tcacaccagc cgccacggct
    13261 tacttgctaa cgaaccattt actgaaaatg atcattacag ctgcaggaat cggtatgcta
    13321 agtgcagttg tcggtgtgtt tttcagttat agttacaatt ggccatcagg agctacgatc
    13381 gtgttagcat gtaccgcatt ttttatcctt gcttttttat tttctccaac aaaaggaatt
    13441 ttatttcaga aaggaagcag atagatgaaa acaaaaaaat cactcttttt aatcttggca
    13501 gttagtttct tggtattagc cggttgcgga aagcaggctt ctgaccaagc ggatgaagga
    13561 agtaaagaaa agttgtcagt cgtggctacc aattcgatct tggcggacat ggcaaaagag
    13621 gtcggaacag atcaaataga tatccacagt atcgtaccag tcggaacaga tccgcatgaa
    13681 tatgaagtac taccagaaga catcaaaaag gcaagcgatg cagatgttat tttatacaac
    13741 ggtttgaatc ttgaaacagg taacagctgg ttcgataact tgatggaaac ggctaaaaaa
    13801 gaagaaggaa aagattattt tgcagttagc aaaaatgtag aacctcttta tttaactagc
    13861 ggtgaagaac atacaaaagc agatccccat gcttggctag acctatctaa cggaataaaa
    13921 tatgtggagg aaatcgcacg tatattctct gaaaaagatg cagaaaatgc gacactctat
    13981 aaaaaaaatg cagaagcata tgtggaaaaa ctaaaagaat tagatacaca agcgaaggaa
    14041 agttttgctt ctatcgaaga gaacaaaaaa ttattagtaa caagtgaagg ggcattcaag
    14101 tatttttcac gagcatatga tttgccagca gcttatatat gggagatcaa tacagaaagt
    14161 caaggtacgc ctgatcaaat gaaagcgatc attgatcaga taagagcaaa agaagtaccg
    14221 gttttattcg tggaaaccag tgtagactca agaagcatgg aacgggtagc aaaagaaacg
    14281 ggtttgaaaa tctatgataa actgttcact gattccatag caaaagaagg agaacaagga
    14341 gattcttact atcagatgat gaaatggaat attgaaacga tccatgaagg tttgtcacaa
    14401 acaaaagaaa gctgaaaagt gcaaggtcta gcttgaggag cagaccttgc attttttata
    14461 agagatatga aaaaaaatta tgctgttttt cgtcgtacag ataccttttt tgaagaaatt
    14521 ccgttatagt tagagtaagg aaggaaggtg tggctatgaa ttttacacgt aaagataaat
    14581 atacgattaa agaagaagtc tttcgattga tcagccagca ggaaaatacc gacaaggatc
    14641 aagaccttca atttcttgaa gcagaattag ctaaattatt catagcaaat caaaatgaga
    14701 caacagaacc agatgaagag ctgtacatag gcaaatggtt gtttgatgaa tttcagcctt
    14761 tagatgaaaa taccggtatc ggttattttg tgttcctttc tccagtcggc ttcacacctg
    14821 gcgtattagt taaacaaaac gacgaaataa ccgtcaaagc atggcctgca ttattttcag
    14881 tcatcaaagc agaaggagta atcaaaaaga tccagtcggc ctattataaa agtcaattta
    14941 ttgccaacag caaattaatg aatatgtttt ataatcgtct agtagaaatt gctaggcctg
    15001 ggaaaatccc gcaaacattc acgtacacac agcaaaaaaa tctctatgaa gaagtattgt
    15061 attcccgcga tctgtctgag ttttcgccat gggaagaggt aaggctccat gaactgttaa
    15121 gtacaaaacc agtttcattg aattttttta atgcattaga taacaaaaga aaattaattt
    15181 caacaaatta ttgggaagta tttgctgcaa gaaaaaaatg gatggaagaa aagaagattg
    15241 ccgatcagca gatccagttt gtccagctga aaaaagaaaa actatattat gggttataca
    15301 gccgttaaca gcaagcgagg aagaccatga cattaaaaga agaaatcata caagaaagca
    15361 aacgattagg aattgacaaa atcgggttca ccacggcgga accttttgat tcattaaaag
    15421 aatcgttgga agaacaaaaa gcagctggcc atacttctgg ctttgaacat ccaaatatcg
    15481 atgagcgtct ttatccagaa atgacatttg atcagccgaa atcgatcatt tccattgcac
    15541 ttgcgtatcc tactaggatc catgaagaag tgcctaggga tgaaaaacgc gggcagtttg
    15601 ctcgtgcttc ttgggggatc gattaccatg atatactcag agaccgtttg aaccgattga
    15661 tcgaattcat ccaaacaaga gcagaaaaat ggcaaaaaga agaggaatgg cgtctagctc
    15721 cgcaagtaga tactggagaa ctgatcgatg tcgctgtagc acagcgagct ggtttaggat
    15781 ttattggacg aaacggattg ttgatcactg aagagttcgg ttcatttgtc tatcttggtg
    15841 agatcgttac taatattgta tttgagcctg ataaaccagg agaatttggt tgcggtgact
    15901 gtatgcgctg cgtgactgct tgtccgacaa aagcattgct tggtgacgga agaatgaatg
    15961 cccagcgatg tttgtcttat caaactcaga caaaagggat gatgccggaa gaatatcgta
    16021 aaaaaatgcg aaatgtcatt tatggatgtg atatctgcca gttagtctgc ccttacaatc
    16081 aaggaaaaga ttttcatttt catgaagaga tggaaccaaa agtagacgaa atatatccaa
    16141 aattaaagcc aatgctttcg atgtctaata aagacttcaa acagcaattt gggcatttag
    16201 ctggttcatg gcgtgggaag aaacctttgc agagaaacgc attgattgct ttagctaatc
    16261 taggtgatcg aacggcattg cctgagattt cagaatgttt aacagatgtc cgccctgtca
    16321 tcagaggaac agcagcttgg gctattggac gtctaggaac aaaagaaccg gaaaaatggc
    16381 ttgatgtatt agatgaaata ttagctaaag aaacagaaga agtcgttata aacgaattgg
    16441 aacatgccca gaaattattg cggagaaagt taaaataaaa aaatagttaa cattattcaa
    16501 agtttttgtc ca
//
LOCUS       DAXWLF010000046        16306 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXWLF010000046 DAXWLF010000000
VERSION     DAXWLF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.046"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(153..551)
                     /locus_tag="ACGE4U_001188"
     CDS             complement(153..551)
                     /locus_tag="ACGE4U_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFF8393365.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="ACGE4U_001189"
     CDS             complement(548..1321)
                     /locus_tag="ACGE4U_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFF8393366.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="ACGE4U_001190"
     CDS             complement(1318..2001)
                     /locus_tag="ACGE4U_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFF8393367.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="ACGE4U_001191"
     CDS             complement(2168..3055)
                     /locus_tag="ACGE4U_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8393368.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="ACGE4U_001192"
     CDS             complement(3052..3921)
                     /locus_tag="ACGE4U_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8393369.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="ACGE4U_001193"
     CDS             complement(4058..5344)
                     /locus_tag="ACGE4U_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8393370.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="ACGE4U_001194"
     CDS             5617..6195
                     /locus_tag="ACGE4U_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8393371.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="ACGE4U_001195"
     CDS             6195..7124
                     /locus_tag="ACGE4U_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8393372.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="ACGE4U_001196"
     CDS             7121..8857
                     /locus_tag="ACGE4U_001196"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8393373.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACGE4U_001197"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACGE4U_001197"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFF8393374.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="ACGE4U_001198"
     CDS             complement(9753..10262)
                     /locus_tag="ACGE4U_001198"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFF8393375.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACGE4U_001199"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACGE4U_001199"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFF8393376.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="ACGE4U_001200"
     CDS             complement(11563..12654)
                     /locus_tag="ACGE4U_001200"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFF8393377.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACGE4U_001201"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACGE4U_001201"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFF8393378.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACGE4U_001202"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACGE4U_001202"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFF8393379.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACGE4U_001203"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACGE4U_001203"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFF8393380.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4699 a         3612 c         2901 g         5094 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatc
//
LOCUS       DAXWLF010000047        16264 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXWLF010000047 DAXWLF010000000
VERSION     DAXWLF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.047"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..456)
                     /locus_tag="ACGE4U_001204"
     CDS             complement(<1..456)
                     /locus_tag="ACGE4U_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8393381.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="ACGE4U_001205"
     CDS             complement(561..1424)
                     /locus_tag="ACGE4U_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFF8393382.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="ACGE4U_001206"
     CDS             complement(1620..3719)
                     /locus_tag="ACGE4U_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFF8393383.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="ACGE4U_001207"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="ACGE4U_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFF8393384.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /gene="dnaG"
                     /locus_tag="ACGE4U_001208"
     CDS             complement(5072..6913)
                     /gene="dnaG"
                     /locus_tag="ACGE4U_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFF8393385.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9410)
                     /locus_tag="ACGE4U_001209"
     CDS             complement(7095..9410)
                     /locus_tag="ACGE4U_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8393386.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="ACGE4U_001210"
     CDS             complement(9595..10455)
                     /locus_tag="ACGE4U_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFF8393387.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11146)
                     /locus_tag="ACGE4U_001211"
                     /pseudo
     CDS             complement(10514..11146)
                     /locus_tag="ACGE4U_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            11313..12074
                     /locus_tag="ACGE4U_001212"
     CDS             11313..12074
                     /locus_tag="ACGE4U_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8393388.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="ACGE4U_001213"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="ACGE4U_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFF8393389.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="ACGE4U_001214"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="ACGE4U_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFF8393390.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
BASE COUNT         4400 a         3410 c         2840 g         5614 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 acct
//
LOCUS       DAXWLF010000048        16102 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXWLF010000048 DAXWLF010000000
VERSION     DAXWLF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.048"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(116..745)
                     /gene="pyrE"
                     /locus_tag="ACGE4U_001215"
     CDS             complement(116..745)
                     /gene="pyrE"
                     /locus_tag="ACGE4U_001215"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFF8393391.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(752..1468)
                     /gene="pyrF"
                     /locus_tag="ACGE4U_001216"
     CDS             complement(752..1468)
                     /gene="pyrF"
                     /locus_tag="ACGE4U_001216"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFF8393392.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1465..2397)
                     /locus_tag="ACGE4U_001217"
     CDS             complement(1465..2397)
                     /locus_tag="ACGE4U_001217"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFF8393393.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2394..3170)
                     /locus_tag="ACGE4U_001218"
     CDS             complement(2394..3170)
                     /locus_tag="ACGE4U_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFF8393394.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3224..6409)
                     /gene="carB"
                     /locus_tag="ACGE4U_001219"
     CDS             complement(3224..6409)
                     /gene="carB"
                     /locus_tag="ACGE4U_001219"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFF8393395.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6414..7490)
                     /locus_tag="ACGE4U_001220"
     CDS             complement(6414..7490)
                     /locus_tag="ACGE4U_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFF8393396.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7495..8775)
                     /locus_tag="ACGE4U_001221"
     CDS             complement(7495..8775)
                     /locus_tag="ACGE4U_001221"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFF8393397.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8794..9720)
                     /locus_tag="ACGE4U_001222"
     CDS             complement(8794..9720)
                     /locus_tag="ACGE4U_001222"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFF8393398.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9733..11016)
                     /locus_tag="ACGE4U_001223"
     CDS             complement(9733..11016)
                     /locus_tag="ACGE4U_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFF8393399.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11030..11569)
                     /gene="pyrR"
                     /locus_tag="ACGE4U_001224"
     CDS             complement(11030..11569)
                     /gene="pyrR"
                     /locus_tag="ACGE4U_001224"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFF8393400.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11804..12715)
                     /locus_tag="ACGE4U_001225"
     CDS             complement(11804..12715)
                     /locus_tag="ACGE4U_001225"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8393401.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12712..13176)
                     /gene="lspA"
                     /locus_tag="ACGE4U_001226"
     CDS             complement(12712..13176)
                     /gene="lspA"
                     /locus_tag="ACGE4U_001226"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFF8393402.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13181..13879)
                     /locus_tag="ACGE4U_001227"
     CDS             complement(13181..13879)
                     /locus_tag="ACGE4U_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFF8393403.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13866..14252)
                     /locus_tag="ACGE4U_001228"
     CDS             complement(13866..14252)
                     /locus_tag="ACGE4U_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393404.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14399..16069)
                     /locus_tag="ACGE4U_001229"
     CDS             complement(14399..16069)
                     /locus_tag="ACGE4U_001229"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFF8393405.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
BASE COUNT         4325 a         3717 c         2877 g         5183 t
ORIGIN      
        1 tttcttttca tacctgatga aaagatgctt ctcgcaatca gtttttggat ttttgctatt
       61 ccaattgatg attctggaga cacaaatcgg atttctctat cttcatcaag cgcgcttaaa
      121 tctgccaatt ttcaggatct tttttccatt cttttagcag agttaattct tgctcatcga
      181 tataacgatc ttctaaagct gcttcaatca atacagaata attggtcaac gtcataagag
      241 ggatttcggc tttttcgaaa tttgcttttc cttttggcaa ctcatacgta aagatagccg
      301 caacaccaag gatatccgca ccttctcttt ttgctgcttc agctgcttcc aagacacttc
      361 cgcctgtaga aatcaaatcc tcgatcacaa ccatcttctg accttcaaat atacgtcctt
      421 cgatttggtt acctttccca tgatctttcg ccttacttcg aatatagacc attggcaaat
      481 ccagtatttc cgctacccat gcggcatgag ggattcctgc tgttgcagtt ccagcaatca
      541 cttgtacttc agggtaagct tctttgattt tgcttgctaa tccttttgcg atctcttttc
      601 ggacttttgg atagctcatc gtgattcggt tatcacagta aattgggctt ttgattccac
      661 ttgcccatgt aaaaggatta cttggattta aaaatactgc ttcaatttct aataagtctt
      721 ttgcgatact ttgttcaaca ttcattttag gttagctcca ttctgtttga atcgtttgat
      781 atgctttata aggattagtt gactgagtga ttggtcgacc tataacgata aaagttgagc
      841 cgatcttttt tgcctgagaa ggtgtgacta ctcttgtctg atctcctgcg actgttcctt
      901 ctggtcttat acccggagtt aagcaaacaa attcgtctcc tgccatttct ttgatcgctt
      961 ctacttccca agcagaagaa accacaccat ctaatcctgc ttttttggca cagctagcat
     1021 aatggatcac acttttttct aaagaaactt cgattagttg atcggcatgc atttcttctt
     1081 cacttgtaga agtaagctga gtgatagcta gaagtagcgg acgttttcca ccttctggag
     1141 tcccctcttc taatcctcgc attgccgcct ccatcatacg aataccgcca gcggcatgaa
     1201 cacaggtgag atcaacacca agctttgcca atccccgcat tgctttttcg accgtattcg
     1261 gaatatcgtg aagtttcaga tctaaaaata cattatgacc agcctctttt agataacgta
     1321 cgatttcagg tccttcttgg tagaacaatt ccattccgat tttcacaaac aatttttctt
     1381 ctttcgggaa aaatcgaaga aagtcttcta cctcttgcct agttgaaaaa tctaatgcaa
     1441 taattggtcg ttggtctagt tgattcatcg ttcacgttcc tctctcactt ctttgattaa
     1501 tctttccagc gattcgatgc ctagttcgct cattcgcaca gggagctctt taattagttt
     1561 aggacagata tacggatcgg tgaaattcgc ggtccctata gctacagcac ttgctccagc
     1621 cataaacatc tcaagaacat catccactgt ctgcacgccg cccatcccaa tgattggaag
     1681 gctggatata cttgctactt ggtgaatcaa acgaatagcg acaggcttga ttgctggtcc
     1741 ggagagtcca cctgtttgat tggctaaaat cggttttctt gtcttaaggt caatccgcat
     1801 accaagaaga gtattgatca tcgtaaaccc atctgcccct ccagcttcga ttgcctgagc
     1861 gattggaacg atgtctgtta catttgggga caatttgaca tagacaggaa cagaagaaac
     1921 tttctttact gcttgtgtta attgaaaagc aacatcagaa tctgttccaa aagcaattcc
     1981 gccatgtttg acatttgggc aagaaatgtt cagctcgatt gctttcacat ttggtgcttc
     2041 gccaattttg ctacaaactt ctacatagtc ttcttcacaa gcaccagcaa catttgcgat
     2101 gatgggtaac tcattgtact cctctaattc tggaagaagt ttatgcataa caacgtctag
     2161 accaggattt tgcaaaccaa ttgcattcaa catgccagat ggtgtttccg ccactcttgg
     2221 cgtgggattg ccaaagcggg catttggagt cgttgccttg accatgatcg atccaagctg
     2281 attcaaatca tagtattttc cgtattcttt gccaaaaccg aaacagccgc ttgccggcat
     2341 gataggattt tttaattcta ggccaggaag tttgaccgct aaaggattct cattcatata
     2401 attacctctc ctattttaaa aatcggtcct tcgtcacaaa ctttcacact ttttgttcct
     2461 gtttcatctt ccggcgtatg acaaacgcaa gcatagcaag cccctatccc acatgccatg
     2521 cgggattcta atgataactg gacattttga tgcgaagaaa acagctgttc cactgtcttc
     2581 aataacccgt ttgaaccaca tgcaaaaaca gctgtaggtt ccgtcttcgt tcctaacaat
     2641 aaattaccga catttccgtg cataccgtat gttccatcgt cagtagcgat tcttgtttct
     2701 cctagacgat taaattcttt ttcataataa acgacttcat gtgtggcaaa acctaaaaaa
     2761 tgcgttggct taattccttt ttcgactaac tgttttgata actcatatag aggagggaca
     2821 ccgattcctc caccgattat aaatacttcg tctcctgtat gaagattgga caaatcaaaa
     2881 ccattgccta aaggacccat gacatctaaa tggtctccgg cagaaagagt agagaagact
     2941 ctagtcccct caccttccac ccgataaatg atacggcaag tatttgtttc gtggtcgata
     3001 tgattcaagc taatcggtcg gcgtaatagc aagtctgcac gcggcacgag aatatgcaag
     3061 aactgtcctg gttgctgcat ttcttttact agatcacctt tcaatgtcat ttcaaaaatc
     3121 tttggtgcca gctgtttttg ctgaacgatt tccatcattt cttgtttcat ctgtcccctc
     3181 ctgcaacggt ttttcgtctt tttgtcttaa catgtgctcg ccgttaaata gggttagttg
     3241 aaaaagcacg ggattccatc actcgaacga tcgcatctgc tgtgtctaaa gaagtaaaca
     3301 atggaatacc atgttcaact gcttctcttc ggatgatgaa accgtcttta gaagcatttc
     3361 cccgatcttt atcaatcgta ttgacaacca cttgtgtttt accatctctt attaagtcga
     3421 ctacattttt ttctgctgct tcctcagaaa ttttcgctac tgtttcaacg accagaccat
     3481 ttttttcgaa ataagcagct gtatgtttcg ttcctatcag actatagcca atttctgtaa
     3541 aacgtttagc taattctagt gcttcttctt ttgtttcgtc cgcaatcgta aataatacgg
     3601 ctccatattc aggcaagtgt aatccagaag cttcgaatgc cttgtacaaa gctttgtcta
     3661 attgtctgtc agatcccatt acttctccag tcgatttcat ttcaggccct aagtaagtat
     3721 caactttctg caatttcgta aaagaaaaga caggtgcttt aacatgaact tcttttgttt
     3781 ctggataaag tccgttttca taaccaagtg tctttagttt ctctcccaaa atagctttcg
     3841 tcgcaatttg tgccattgga attcctgtca ccttacttaa gaatggaaca gtccgacttg
     3901 ctcgaggatt gacttcgatc acataaacct cattttcatg gatgacaaac tggatattca
     3961 tcatcccgac acaattcaaa cctaatgcta agcggatcgt gtaatcttcg atcgttttct
     4021 tgatcgattc agataatgtt tgaggtggat acacagccat tgaatcccct gaatggacac
     4081 cagcacgctc aatatgctcc atgatccctg ggatgagtac tgtttcaccg tcacaaatcg
     4141 catccacttc gcattcacca ccaagcaagt aacggtccac tagtactgga tgttctggag
     4201 atgctttcac agcatgttcc atgtaatctt ctaaatctcg ctggttttca acgatttcca
     4261 tcgcacgtcc gcctaataca tagcttggac gaacaagaac tggataacca attctcgaag
     4321 cgataaccac ggcttcttct ttacttgtag cagtatcccc taaaggttga ggaatatcca
     4381 actctttcag cgcttgctcg aataaatcac gattttccgc acgatccaag tcttcgatcg
     4441 ttgtccctaa gatttttaca ccagcagcag tcaaaggttc ggctaagttg attgctgtct
     4501 gaccaccaaa ttgaacgatt actcctatcg gctgttctag ctcgatcaca ttcatcacat
     4561 cttcgaaggt caatggctca aaatataatt tatccgaaat agaaaagtca gtcgaaactg
     4621 tttcaggatt gctgttcatg atgattgctt cgtatcctgc ctgctggatt gctttgacag
     4681 aatgcacagt cgcatagtca aattcaaccc cttgtccgat acggatagga ccagatccca
     4741 gtactagaat tgagggcttt tctgatcgtg aactttcatt ttcatattca taggaactat
     4801 aaaaataagg tgtttttgat tcgaattccg ctgcacaagt gtcaaccatc ttatatacag
     4861 gaatcaaatc attttctttt ctcatctggc gaacggcttc ttcgtttatt tcccacagct
     4921 ccgccacttt tttgtctgta aaaccgtttt gtttcgctgt tttcaatatc ttgtcatcca
     4981 acggctgctg tttcaactgc tgttcgattt ctacgatatg caacagttta tctaaaaaga
     5041 ataaatcgat ttttgtcaaa tccgctaatt cttctatggt gtaccctctt ctaatagctt
     5101 cagctacata aaacaggcga tcatcttgtg ctcgaaccac tttttggatt aggtcatcgt
     5161 ccgtgatgtc tgccaactca ggaagttcga tatggtgtgt accgatttct aaagaacgca
     5221 cagctttcaa taaagactct tcgatattcc gaccgatagc catcacttca ccagttgctt
     5281 tcatctgcgt gcctaaaagc cgttctccgt tttcaaactt atcaaacggc cagcgaggaa
     5341 ttttagctac aacataatct aatgcaggtt caaattctgc aaaagtcgtt cctgtaacag
     5401 gatttttcat ttcgtctaat gttaatccaa gcgctatttt agcagctagc ttagcgatcg
     5461 gatagccagt cgccttactt gctaacgcag atgaacgaga aacacgcgga ttgacttcaa
     5521 tcacataata attaaaacta tgtggatcaa gagcgagttg gacattacag cctccctcga
     5581 tcttaagtgc tcgaataatc gaaagagaag catcacgaag catttgatat tcatggtcag
     5641 acaatgtttg actaggggca aatacgatag aatcacctgt atggataccc accgggtcaa
     5701 aattttccat attgcagaca acgattgcat tatcagccga atctctcatc acttcatatt
     5761 caatttcttt aaaacctgca atacttcgtt caatcaaaca ttgggtagcg ggggaaagac
     5821 ttaatccatt ttctgatacg atacgtaatt cttcttcatt ttcacacata cctccaccag
     5881 ttccgcctaa agtaaaggct ggacgaacga tgacaggata gccgatttta tttgcaaaat
     5941 ctacagcttc ttctactgta ttgacgattt cagactcggg gattggctga ttcaattctt
     6001 tcatcaactg tttgaataga tcacgatctt ctgcttgatc gatcgcagaa agctttgttc
     6061 ctagaagttc tatgttcaat tcttctaaaa tacctgatgc tgccagctcc attgccatat
     6121 tgaggcctgt ctgtccgcct aaagttggaa ggagagcatc tggacgctct tttcgaagaa
     6181 tccttgatac aaattcaagt gtgatcggtt cgatatatac ttgatctgcc acttctttat
     6241 ccgtcatgat cgtcgcagga ttagaattta ctaaaaccac ttcatatcct tcttctttta
     6301 aggcaaggca ggcttgtgtt cctgcgtagt caaattctgc ggcttgacca atgatgatcg
     6361 gaccagatcc aatcaccata attttcttga tatctgtacg tttcggcatc tgtttactgc
     6421 tcctttcctg cgtccatcat ttccataaat tcatcaaata aatgtagtgc atcatgcgga
     6481 ccaggggcag catctggatg aaactgcaca ctgaaagcgg gataatctct gtgtttgact
     6541 ccttctactg ttccgtcatt tacttctaca tgagttacga tgagtttttc tggatcaatc
     6601 gtggcttcat cgactgcata gccatgattt tgagaagtaa aatcaattct tccggtcgct
     6661 acttctcgta ccgggtgatt taatcctcga tgtccgaatt tcattttata ggtatctgca
     6721 ccgttggcta aagcaaataa ctggtgaccg agacaaatac caaaaatcgg tacttttcct
     6781 tggatttttt tgatcatttc gattgcttct ggcacatctt ttggatcacc aggtccattc
     6841 gtcagcatga ctccgtctgg tgatagcgct aaaatcgtct cggcatctgt gttataggga
     6901 agtactgtga ggttacagtt tcttttactc aattctcgta aaatactatg tttcaagccg
     6961 aagtcaatga caatgacttt ccgacctgtt cctggacttg gataaggttt gttagttgag
     7021 acttgctgca cttgattggt tgctagaacg gttgctttca gctgatcaaa tgcatgttcg
     7081 aaggaatctc cagcatcaac gatactagct ttcatcgttc cgacttcacg gattttgcga
     7141 gtaagggcgc gcgtatcgat acctgatatt ccaggaattc ctttttgttt caaaaatgaa
     7201 tctagcgtca tttggtttcg ccagtttgaa gctacacgtg catgttcttt tactactact
     7261 cctttgcatg ttggagaaat cgattcataa tcatctctat ttaccccata attacccacc
     7321 ataggatagg taaatgtaat gatttgaccg ttaaaacttt gatcagtgat agtttcttga
     7381 taaccagtca tacttgttgt aaaaacgatt tctccgaaga cactagcatc tgcaccaaaa
     7441 gcctttcctt cgaaaactgt tccatcttct aatataagca aacgttccat acttttattc
     7501 tcccttctgc cacacaagtt ttccgttgac aaaagtcatt tgtgtttcgc cttttacttt
     7561 ccagccgata aaaggtgtat tctccccttt tgataaaaag tcttctttat cgatcgtgca
     7621 agtttgtgta atatcaaaaa ctgctacatc agcgggggca ccaaccgtta gcgttcctgc
     7681 attcaaacca aaaatctcag ccggtttaac tgccaaccaa ttgatcactt gttccaacgt
     7741 gaatcgacca gtttctacaa aatgagtata gatcagttga aacgctgttt cgcttcctac
     7801 gataccaaat ggtgatttca tgaaactttg gcttttttct tctaatccat ggggggcatg
     7861 gtctgtagcg atgcaatcga tcgtaccgtc aagcaagcct tcgatcaaag cttctctgtc
     7921 ctcccgtcct cttagcggcg ggttcatttt ccaaaagccg aaatcttcgg gaatatcttc
     7981 gtcgatcaaa ataagatgat ggggtgaaac ttctgctgtt acgtggattc ctgctttttt
     8041 tgcgtctctg attacacgga cactttcttt agtagaaaca tggcaaacat ggtaatgaac
     8101 accagttgct tcagctagca acagatctct agcaatttga gaagactcgg ttacgcttaa
     8161 gatcccggga agaccaagtt cttctgcctt cttgccagcg tgcataacgc cgccaaataa
     8221 taaagattca tcttctgtat gggctactag ggctttattg ttttttgcag cttctttcat
     8281 agccagatac atcgtcccag ctgtttggac acctacaccg tcatttgtga aagcaaaagc
     8341 accttcttca atcagtgctt cttgatcaac taatttctca cttcgcagat tttctgtgat
     8401 tggcgcatac tgcaatacct tcacaactgc atcttttcta atgatgtcat atacttgtct
     8461 cagcttttct gccgtatcag gtacaggatt taaattcggc atcgcgcata cagtagtgaa
     8521 accgccacga gcagctgagc gagtcccagc ttcgatcgtc tccttgtatg taaatcctgg
     8581 ctctctcaaa tgaacatgga cgtccactaa accaggtgtg atcaattgtc cctttgcatc
     8641 aaacacttca tcaaattcgg cttctgagaa tcctgtaccg attgctttga ttcgcccatc
     8701 ttctatccaa atttctgctg gtgtcgtcat attttttcgt gtattgatct gaccgttttt
     8761 aatgagtgtt ttcatttttt catcctccta taattacgct tttccattta gcactgcttc
     8821 taagattgcc attctgacga atactccatt gctcatttgc tgaacgatcc gcgactgtaa
     8881 tccttccact aatgaatcag ctaattctac atcacggtta actggtgctg ggtgcataat
     8941 gattgcatgt ttttgcaact tcttcgcacg ctcaactgtt aacccatgtt cctgatggta
     9001 gccttctttt gagaaacttt cttttccatc atggcgttcg tgttgcaccc gaagcatcat
     9061 catgacatca acttgatcta gtagttcatc caatggcata taatgtccgt acacttcgaa
     9121 ttcatcgtcg taccattctc taggacctga gaagaatact tgtgcgccta gacgttttaa
     9181 catctgcata ttggatttag ctacgcgaga gtgagtgata tcacctacga tggcaacttt
     9241 cagaccttca aaatgaccaa attcttcata aatggtcatc aaatcaagca agcattgggt
     9301 tggatgctgt ccgcttccgt ctcctccatt gataatcgaa cactggattg ttttactttg
     9361 tatcagttcg tcataatagt tctcatcacc atgacggata accgctacgt ctacgcctaa
     9421 tgctgacatc gtcagtactg tatcgtatag tgtttcccct ttttggacag agcttgtgct
     9481 tgcttcaaat tcaatcactt ctagccctag ttttttctcc gctacatcga agcttttatg
     9541 tgtgcgagta ctattttcga aaaagaggtt tgtagcaaag tactggcgct tttgcggagt
     9601 ccaattagca ccttgcttga attcctgccc tctgcggatc aagcccataa cttcttgatc
     9661 agtaagtgcc tcaacggtca ataaatgctt taagctgata cgttctgatt tgatgatcat
     9721 aatgtttcct ccttagtttt cttcgcttcg tgaagtttct ggtaagataa gatttaaaat
     9781 gatgccaagt acagttgcta atgccattgc cgacaatgta aatgtgccta cttcaaagac
     9841 gagtccgcca attcctacta ctaagataac tgaagcaatc aacagatttt tctttttatc
     9901 aaaatcgatt ttgttctcga ttaaaatctt cattccactt gcggcaatta ctccaaataa
     9961 gataaaactg attcctgaga tgactggtcc agggatactt aaaatcaaag cgcttaattt
    10021 tcctacgaat cctaaaatga ctgcaaatac tgcagctcca ccgatcacaa acacgctatg
    10081 gacacgtgtg atagccagaa caccaatgtt ttcaccgtaa cttgttacag gaggacctcc
    10141 gacaaaggct gccacgattt gtgctaatcc atcacctgtc aacgtcttat gaagacccgg
    10201 ttcttcaaag aaattacgtt tagttagttt attcaatacc ataagatggc cgatatgctc
    10261 tgtcatcgtc acaaaagcga tgggtgccat tgttgtgatt gctccaagat atagctttgg
    10321 ttcatattgt acaaagggaa tctcaaagtt aggaacagca aaccaggcag cgtctacgat
    10381 tggcttagta tccacgattc caaacagcaa ggctacgatg tatccgctaa taatacctag
    10441 taaaatcgga atcaatccga gaaaacctct tagccacatg ttgaagaaaa ttgttaatcc
    10501 taatgtgatc agcgctacag caatatactt gaaatcatac gtgccctgat tatacatagc
    10561 atttgttgct gcatttgccg caagtcccaa cccgataacc atgatgactg gaccgacgac
    10621 gattggaggc aagattttat ccagccatgc tgaaccgatc tttttgatga tcagcgcaac
    10681 aatcaaatag actaatccag ttgtgactgc tccttgtgca atcgctggat aaccatctgt
    10741 tttcatcaaa agctgcatcg ctgcaatgaa agcgaagctg cttcctaagt acgcaggtat
    10801 ctttccttta gtagtaaata agtacaccaa tgtacctagt cctgaactga ctagcgcaat
    10861 acctggatcg atacctacta agatggggac taacacggtt gctccgaaca tcgtgaacaa
    10921 gtgttgtaaa cttaatccta cccaatgtcc tggggttggg cgatcgtgga tatctaacac
    10981 cacgtcatca ttatgaaatc cttttttctc cgacatgttt ctctctcctc taatcttcct
    11041 ctttcaaaat catgatgcga tcttgtccat caagctcttg catctctact aaaatctctt
    11101 ccgcccgaga tgttggaatg ttcttcccaa cataatctgc acggattggc aattctcgat
    11161 gcccacgatc gaccaggact gctaatgaaa tttttcttgg tctgccaaaa tccattactg
    11221 cgtccattgc tgctcgaatc gttcgtcctg tatacaaaac atcatcaatc aaaataactt
    11281 cttttccttc taaggaaact gggatgtcag aagaatgaag ttccggttct tctggatttt
    11341 cttttttgtc atctcggtat aaagtgatat ccagttctcc tacaggaata tcgatatctt
    11401 ctagctgctt caggcgttcg gcaattcttg atgcaatgta gattcctcgt gttttgatac
    11461 ctaccaatac gatatcttgg atactatggt ttctctcgat aatctcataa gtaattcgag
    11521 tcaatgctcg cttcatggtt acctgatcta ctacttcttt tgcttgcatt ttcattcccc
    11581 ctactttttt gcataaaaaa actcctgccc taagaggaca ggaggaatgt ttagactgag
    11641 cgtatactga tacactgata gctatactat gtcaatgagc aatctactta tggcgcacca
    11701 tacgtatcct tgctcccacc cttcttagcc tcacgggact aaacttaaag gaattctatt
    11761 aagttttatg ctttcgttgt taattactat accgttatct aaattaaaat gcaatgtttt
    11821 ttcttaattt ttctaaagtt ttttcaaaaa cttctggaag aggtgcttca aagatcatct
    11881 tttcgtttgt agtcgggtgc gtaaaaccta acatttgcgc atggagaaac tgaccattgc
    11941 ctttcaaagt tttcttcggt ccgtatactg gatctccagc taacggataa ccgatatact
    12001 tcatatgcac acggatctgg tgtgttcttc cggtttctag ttttaattca accagagtaa
    12061 atccttcgaa acgttccaat acagtaaaat gtgtgacagc cgacttccca tcttctctta
    12121 tcgcctgcat tttccggttt tccttcgaac ggccgattgg cgcattgatg gttccttttt
    12181 cgtgaggaat ctctccatgg actaaagcaa catatttgcg caaagaggtc ttatctttta
    12241 attgctgagc taatgattcg tgtgcctggt catttttggc aaccattagc agtccggaag
    12301 tatctttgtc gattcggtga acaatacctg gacgaataac gccgttgatc gtagaaagat
    12361 ccttgatatg gtacatcagc gcattgacca ttgtgccatg cgtatgacct gctgaaggat
    12421 gaacgaccat tccttgaggt ttgtttatca ccgctacatc ttcatcttcg taaacgatcg
    12481 tcaacgggat attttctggg accacatcta atgtttctgg ttcggggatc aagacagtaa
    12541 tctcgtctcc ttgttttact ttgtaatttg ctttgactgg ctgtccattg acagaaaccg
    12601 ctccattttt cagccacagc tgaatttgcg aacgactgta atcggtgaat aaattagtca
    12661 atactttatc gatcctacca gattcatggt gaatcgtcgt tttaagttct gtcatttttt
    12721 tctttgatcc ttttcatcta aaataatata aattaacata caaatcactc cacatacaag
    12781 tgtcacatct gctacgttaa aaatcggaaa gctcataaaa tctgtctgga acatatctac
    12841 tacatagcca agacgaatac ggtcgataaa attgccaatc gcaccagcta gaatcaaact
    12901 caatccaatc atgaaccagc gattaccttt ttcacgattt ttaatcatca atgtgagaat
    12961 cacaacgact gcaatgagtg tcactatagt aaagaaccac atcttccctt ctagcatact
    13021 ccatgcggct ccagtgtttc gaatatgatt cagtgacaaa atacctggta taagattttt
    13081 cgtctctcca agcgctagat tatcagtgat ccaccatttg accaactgat ccactcctac
    13141 tagcaaagcg ctgaatatcc aataaataat taacaaaaaa tcaatccttt tctatttgtt
    13201 tataaatatc acgaggtcgt acgatcccga gcagattacc atcgggattc ccatctttag
    13261 tgataagtac ggcttccaaa gtcccttgct cacgaaacat cgcttcaacc tcgtaaatat
    13321 gtgttttagc tggaacaaaa tgataatttg tgtactttcc gttttggatg atcaaatcca
    13381 acgctcgttt atttgttaaa tcaaccggac ctttttggct ttcttttgct agccaaaacc
    13441 cgatattacg taaagtaatc aatgctacaa attttccttt attgtataac ggaaattgtg
    13501 aatacctttt ttctgctact atctttaaca gttcaagtaa cggaagatct tgttcaaaac
    13561 ctgttacacg ttttgcaaat cgaggcagca cggtttcagg ctgaatcagt tcttgttcga
    13621 tcagctggat tcgcttgact gtccaatcat ttggctctgc aatgacaaaa tcgactgcga
    13681 tccgatcatg gacgatcgca ttccttaact gagcaagctg taaaagatca tcttcgtatt
    13741 ttctgatcat taattgtttt ctttgagcta ggcgtctgac aagttcggta aatcccatat
    13801 ttcgtgcatt tcccatctcg tctctcatcc atttttcgat tcgattgaaa ctgattaaaa
    13861 aaagatcaga gtttgccata attttcctcc ttgatcaaat atgcccggtt ttttatatag
    13921 aaaaagaacg caatcaacac aataactgca aaaaaactga aaaataaatt ttgtaggaaa
    13981 acggccccca acccagttgc tagaaataaa aacggataga tttgaagaat caagtaattc
    14041 attgtgatca cataagcagg ctttgcttct ttcactgtat aaacggggct ttttagccaa
    14101 taaaaggtga cctgcatcgt aaagggcact tccagatcaa gcgttatctt ttgttcatga
    14161 ctgagaacac tgcgtttttc attgttgatt cttagctgaa tcgagctacc cataccgtaa
    14221 aaaccggttt ttcttataaa tgtcgttttc atcataccct cctattatgc tttagtatag
    14281 ctcaaaatac aataaagaaa aagaatgatc ctagtgtaaa caaaattaat tccctatcgt
    14341 ctaaaaaaag aagctgtagt caaaatacag ctccttcgat ggtagattat aatcaagctt
    14401 aaaacaatcc caacacttct ccgtcatttg tcacgtccat attcaatgca gcaggttttt
    14461 taggcaaacc aggcatcgtc ataacatctc cagtcaaggc tactaaaaat cctgccccta
    14521 attttgggat gatttcgcgg atcgtgatgg tgaaatcttc cggtgcacct agcagtgaag
    14581 gatcatcaga aaatgaatac tgtgtttttg ccatacaaat cggcagtttg tcccatccat
    14641 tcttcgtaaa ctcttctatt tgtttttgcg cttttttgct gaaaacaaca ccatttcctc
    14701 cgtaaatttt cttgacgatt gtttctgtct tttcttgtat cgtttcttct aggcggtata
    14761 atggcttgta attagcttct tttctatcaa tcaatcgtac tacagctttt gctaaatcaa
    14821 cgccgccttc tgctccatct gcccatacac tcgctctctt ggcaagaatc ccctgatttt
    14881 cacacaagtg ctccaacagt gtgagttcat tgtctgtatc tgttacgaat tcattgattg
    14941 cgacgatcac aggtagctga tattgttcca tattacgtat atggcgcttt aaatttgcaa
    15001 aacctacttt caaagcatca agattctcat ttttaagttc atcttttttc attccgccat
    15061 gcattttcaa tgcacggaca gtggcgacaa tcaccacagc atccggcgct tttttcaagt
    15121 taggtacttt gatatctaaa aatttctctc cgccaagatc tgcaccaaat cctgcctctg
    15181 tgacaacgta atctgctaat ctcaatgctg tcttggtagc taaaatacta ttgcagccat
    15241 gagcgatatt ggcaaacggt cccccgtgga caaaagctgg tgttccgtaa attgtttgta
    15301 caagattcgg cttgattgca tctttcagca gtaatgccag agcgccttct gctttcaagt
    15361 ctccagcagt cacaggctga cggtcaaatg tatagccgac aacgatcctt gcaagacgag
    15421 cctttaaatc ttcaaggtct gccgcaagac acaagatagc catgatttcg ctggcaactg
    15481 tgatatcaaa cccatcttct cttggaacac cttggattgg cccacccaag ccaacaatta
    15541 ctttacgcag ttcacgatca ttgagatcga ctacccgttt ccaaatcact cggcgggcat
    15601 cgatgttcaa ttcatttcct tgatgaatat gattatctaa aagagcagat aatgcattat
    15661 tagctgttgt gatagcatgc atgtcaccag taaaatgcaa gttgatatct tccataggca
    15721 atacctgtgc atatcctccg cctgcagcac cacctttgat tcccatcaca ggaccaagtg
    15781 agggttctcg caatgcaata acagattttt tattgatttt attgagcgcg tctcctaacc
    15841 ctacagtgat cgttgacttt ccttcgccag ctggtgttgg attgatggaa gtaactaaga
    15901 tcaaatgccc ctcagcgtta tcctttgatc gattgattcc agaaaaattg attttcgcct
    15961 tatatttccc atacagttcc aaatcgtctt ccttaagatc taatttacta gccacttcaa
    16021 caatcggttc tagctctgtt tcttgtgaaa tttgcagatc attcttcatt tgattacccc
    16081 ttttcacgaa ctttattcaa ca
//
LOCUS       DAXWLF010000049        15851 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXWLF010000049 DAXWLF010000000
VERSION     DAXWLF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.049"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(3..1304)
                     /locus_tag="ACGE4U_001230"
     CDS             complement(3..1304)
                     /locus_tag="ACGE4U_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFF8393406.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(1534..3525)
                     /locus_tag="ACGE4U_001231"
     CDS             complement(1534..3525)
                     /locus_tag="ACGE4U_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFF8393407.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(3669..3935)
                     /locus_tag="ACGE4U_001232"
     CDS             complement(3669..3935)
                     /locus_tag="ACGE4U_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8393408.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(4445..6571)
                     /gene="pnp"
                     /locus_tag="ACGE4U_001233"
     CDS             complement(4445..6571)
                     /gene="pnp"
                     /locus_tag="ACGE4U_001233"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFF8393409.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(6759..7028)
                     /gene="rpsO"
                     /locus_tag="ACGE4U_001234"
     CDS             complement(6759..7028)
                     /gene="rpsO"
                     /locus_tag="ACGE4U_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFF8393410.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            7279..7842
                     /gene="def"
                     /locus_tag="ACGE4U_001235"
     CDS             7279..7842
                     /gene="def"
                     /locus_tag="ACGE4U_001235"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFF8393411.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(8095..8616)
                     /locus_tag="ACGE4U_001236"
     CDS             complement(8095..8616)
                     /locus_tag="ACGE4U_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFF8393412.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(8619..9461)
                     /locus_tag="ACGE4U_001237"
     CDS             complement(8619..9461)
                     /locus_tag="ACGE4U_001237"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFF8393413.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(9454..10179)
                     /locus_tag="ACGE4U_001238"
     CDS             complement(9454..10179)
                     /locus_tag="ACGE4U_001238"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFF8393414.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            10355..10651
                     /locus_tag="ACGE4U_001239"
     CDS             10355..10651
                     /locus_tag="ACGE4U_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393415.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      10907..11081
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            11219..12811
                     /locus_tag="ACGE4U_001240"
     CDS             11219..12811
                     /locus_tag="ACGE4U_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8393416.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            12824..13564
                     /locus_tag="ACGE4U_001241"
     CDS             12824..13564
                     /locus_tag="ACGE4U_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8393417.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            13732..15081
                     /locus_tag="ACGE4U_001242"
     CDS             13732..15081
                     /locus_tag="ACGE4U_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8393418.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(15209..15799)
                     /locus_tag="ACGE4U_001243"
     CDS             complement(15209..15799)
                     /locus_tag="ACGE4U_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFF8393419.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         4564 a         3272 c         3030 g         4985 t
ORIGIN      
        1 ttttattcta tttttctttc cgtggactct actttgaaat aatcgatatg tgcatgctgg
       61 tattctaata ccgtgaaacg gaatgatgca atctcaatga ttgttcctgc ttttatttct
      121 ttatatcggc tagaagcaaa gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga
      181 atctctacat gaaaataacg ctggaaatca tctaatgtta gtttgccgga gatgatataa
      241 gagccatctg actgttccgc tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc
      301 ccgacgattt cttctaaaat atcttcaatc gtcactaagc cgcttgtccc tccgtattca
      361 tcttttaaga tagcaatatg ctttcccttt tttcgcatga tcgtcaataa tttttttata
      421 gggatcgttt cagttgttac gatcggctct tcaatcaact gttctactgt cagctggtca
      481 tctttataag attgcttgat caaatctggc aaggtgacat aacctaaaac attgtctttt
      541 gattctaaga taaccggata gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct
      601 ttgacggttt ctgaagcttc gaatacttgc atggacgtac ggtctacttg tatatctttt
      661 gccaaggtat catcaaattc aaacacgttt tccaaataat gatattcttc tttggtcaac
      721 tctccttttt cataagaatt caatgcgact aatgtcagct cttcttgaga caatgtctct
      781 tctccttcag atacgagacg cacacctaat agcttcccta gccctgcagc tgaatgattc
      841 aatacccaaa taaaaggaaa cattacttta taaaaataat gaagtggttt aactacgaac
      901 aagacgacct tctccgtatt gacgatacta tagctcttag gaagcagttc gccaataacc
      961 acatcgatat acgtaactaa aataaatccc agcgccacag agattgtact gctaatagcc
     1021 gaactaaaag gtagtttacc gatcactggt tctagtaaag ccgcgacaga tccttcaccg
     1081 acccacccga tgatcaaacc ggttagcgta attcctaatt gactagctga taaatagtta
     1141 tctaaatggt gtgtaacatg aagagctagc ttggcgttct ttacattttc cttttctagt
     1201 tgttccaatc ttgatcgtct gattttcact aaagcaaatt cacttgctac aaaaaatgaa
     1261 gtaattgcaa tcatcaaaaa taaaataatg aaattgataa ccatattctc ccgcctttct
     1321 ctttttctat tgtacccttt ttctacaaaa agattgaaaa taatgcgtat tcatatttag
     1381 gaataaaaaa ggagatgcgg catttttcag ccacatctcc ttatcacttc aaaatcattg
     1441 cgttacttgt tgctaaacag ctttttcatg acctctgata aacggtgttc aagtagctga
     1501 cattcgacat tagttcaggt caaagcgcta ctttcacaac cgctctttca accatgtttt
     1561 ccgagtattt ccaatgataa gcggaacagt ttcatgtttt acttcgctgt actctagccc
     1621 aaaccatgtt tctggcgagg tagcgatttt tttgattgcc agttttgcct gcatgatttg
     1681 acgttgccat tttcctagag ttgtcacatt ggaaactact tcatcaatca ggacaaattg
     1741 gaaatcgcca acttctcgcc ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg
     1801 gccttgtttc atcaaatcat ggatgacttg gcgaacatat acgttgattt cctgttggac
     1861 cctgaaacct aagaacagat tcacttggac aataaaatca gtgtccatca tatctacttg
     1921 atattctttc gtatatggct catccgtcac ttccacatga acaaaccaat agactcgtgc
     1981 tcttttaggc cgtttatcta aaatcgaata gatgaactgt cggccaactt tatcttcagc
     2041 catatcagga accaagaaga ccacatttgt ctgatagatt ggtaaagaag tatcatcacg
     2101 taattctcta agctgcggga tataatctct caaagacaca tcctcggttg acgattcgcg
     2161 aataatgttc ccttgttccc aaataaacat cacgactaag atgatcaaac caatcagaac
     2221 ggctacaaaa ccgcctcgga agaatttgac aacacttgag ataaagaaaa cgacttctat
     2281 cgcaccgaag aaaaaggtaa ctaaatatgc aagccaacga gaatgcccat tttggataag
     2341 ataaaagtaa agcagtgctg ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc
     2401 agcttccata tgatgagacg ttcggaatgt gacaaccact agagaacagg cgatccataa
     2461 aatcagattg actgctggga tatacatttg cccgatactg ttcccaggat acatgatctt
     2521 taaccgaggc agcagtttca acttgattgc ttctgaaact agcgtaaacg aacctgtaat
     2581 caatgcttgc gaagcaatga ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca
     2641 actttgaggc atcatttgga agaatggatt caggttcttg atttgctgtg cttcagggtt
     2701 ttgataaaca ttcaacaacc acgcagcctg cccaaaataa ttgataacca aacaaatttt
     2761 gacgtaaggc cagctcagac gaatatttcg tttgccgaca tgtccaagat cggaataaag
     2821 cgcctctgca cctgtcgtag ccaggaaaat atttccaaga atgaacaagc ctaatttgtt
     2881 atctgcacta aaaagcagct ggatagcata ataaggattc agtgcacgga taactgtcca
     2941 gtcttgactg aagttcatta atcccattac gcctaaaaaa gtaaaccaag cgaacatgat
     3001 tggtccgaat gcttttccga caatatctgt accaaagcgt tggaccatga ataaaacaaa
     3061 aataataact aaagtgataa tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa
     3121 ctgcggaatt cctcgcaagc cttcaatcgc agtcgttacc gtcacagcag gggtcaatac
     3181 cccatctgct agtaaggcag ctcctccgat cattgcagga atgatcaaat atttcccacc
     3241 ttttcggaca agtgtgtaga gtgagaaaat tccaccttca ccatggttat ctgcattcaa
     3301 tgcaatcacg acatatttga ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac
     3361 tgatccgata acaaaatcag gattgattcc tgacaatccg ccattatctt caacaatcga
     3421 tttcataacg taaagcggac ttgtcccgat gtctccgtag acgactccca tcgctacaag
     3481 caggccagcc attgatatct ttttggtatt gtgcgttgtt gccacgtttt tccctcattt
     3541 cttgttatat ggatatcctt taggtaagct tgaatgatag atgatattct tatccatgac
     3601 acccgctttc ctaaattgct tctaattatc caagtaacac actgaagtgt caacttattt
     3661 gaataaaatt attctttctt tggacgttct ctgatacgtc ttgccacaaa tgaataaaga
     3721 gcaaggcaaa aaattgcacc caataatgct ggtatcaaat aaaaaccgcc gagttccggt
     3781 cccagtggtc ctagtaaaaa ttcgccgagc caactaccta aaaaaccgat aatgatattt
     3841 ccagatacgc ctcccgggac ttcctttcct aaaattgcgc cagctatcaa acctaatata
     3901 ccgccgataa ataacgacca taaaaaattc agcatctcct ttccctcctt tatccatatt
     3961 tctcactttt attttacgct ttattcagaa ggactccaaa aatatgcgcc aagaaactcc
     4021 agaaaaaaag aagttgtaac aaaagtgttc agcttatttc cgtagaagct gcttctggaa
     4081 caccgtgaat cagaggttgt gaaatcagcg gtttgacctg agtattagag aaacaaacgg
     4141 aaaaatagtt cctcatattt tgccgttttt tgagctaata tcgagggaca gctgattgaa
     4201 catcgtttat tggagaggtt gtaaaaaagc tgttctgcat caagtaataa gaacagccaa
     4261 gtaaaaacag ctcctaatgt ttttaacttg gctggactta ttaccgcaga tgcaagcttt
     4321 tgaacaccgt ttattcgaaa aaaaaggact gtgacacggc ttttgtcaca gtcccctttt
     4381 ttcgaataat tattgtcccc ctgtcacctt cacgatcaca agtacattat tcctatgaat
     4441 taaattattt ttcttcattt ttcgttgaac catcttcgtt caacattgct tttcttgaca
     4501 gatttacgcg tccttgttta tcgatttcag ttactttcac aaggatctca tcacctagtt
     4561 taacaacatc ttcgacttta ttgacacgat cgtttgctaa ttgggaaata tggacaagtc
     4621 catcttttcc tttgatcaag ttgacgaatg caccgaattt ttcgatacgg acaactttcc
     4681 ctagataaac ttcaccaact ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt
     4741 ttttgatcat ttctgcatca gctgaagcaa tacttacttt tccatcttga tcgatatcga
     4801 ttttcacacc agtttcatcg atgattccgt tgatcgtatc gccgccttta ccgataacat
     4861 ctttgatttt agcaggttcg atctggatca tttcgatttt tggtgcatat tggcttaatt
     4921 cttcacgtgg agcagcaatc gttgaagtca attcttctaa gatttccata cgtgcttgtt
     4981 ttgcttgagt taatgcttca gttaggattt gttctgtgat tccttgaatc ttgatatcca
     5041 tctgtaaagc agtgatacca tcttttgtac cggcaacttt aaagtccata tcgccaagat
     5101 gatcttccag cccttggata tcagttagga tcgtatagtt ttcaccatca gaaacaagtc
     5161 ccatcgcaat accggcgact ggcgctttga ttggtacacc tgcatccatc aatgctaatg
     5221 tgccagcaca gatacttgct tgggaagaag aaccatttga ttccaatact tctgctacta
     5281 aacggatcgt ataaggaaat tcttcttctg aagggattac ttgtgctaac gcacgttcac
     5341 ctaatgcgcc atgtccgatt tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa
     5401 cagaaaattg agggaaattg tagtggtgga tgaatcgttt gctgacttcc acccctaaac
     5461 catcaatgat ttggtgctct cctagaggtg cgagtgtaca agctgataaa gcttgtgttt
     5521 gtcctcgtgt gaacaaacct gatccatgta cacgtggcaa taagctgacc tctgatgaca
     5581 atggacggat ttcatccaat ttacgcccgt caggacggat cttatcgatg gtgatcaatt
     5641 cacgtacgac atctttttca agatcttctg tgatttgttt aacttctttc aacaagtgat
     5701 cttcatcttc atgacctgca aatttctcag cataagcttc gcggatatcg atcttcacct
     5761 gctcgatatt atcttcgcga gcaagttttt cttcagtcat tactgctgct ttcattgtag
     5821 cgtaagaagc atcaaagatt tcttttttca atacttcatc aacttgcagt aaagtcactt
     5881 ccattttttc tttccctact gcttgaacga tttcttcttg gaaagcaact aactctttga
     5941 tagcgtcaaa accaaacagc aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc
     6001 cgctttcaac catattgatg gcttgtttcg ttccggcaac agtcaattcg atatctgttt
     6061 gttctgcttg ttctactgtt ggattcaaga cgtactcgcc atttacacga ccaacttcta
     6121 cgcctgcgat tgggccgtca aatgggatat ctgagattga tagtgctaaa gaagaaccta
     6181 gcattgctgc catagctggc gagcagtctg tttctacact catcacgatg ttcgttactt
     6241 ggacttcatt acggaagcct tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg
     6301 cagtcaatgt tgcttccgta cttggacggc cttctcgttt gatgaaacct ccagggattt
     6361 tccctgctgc atacattttt tcttcataat tgatcgttaa tgggaaaaag tctgtatctt
     6421 ttgcttcttt agaggccaca gctgcactta agactactgt atctccataa cggactaaaa
     6481 cggctccgtt tgcttgtttt gctagctgac cgacttcgat ttgtaaggga cggccgcccc
     6541 atgtcgtttt gaatacttgt ttttctgaca taacttctcc ttttccttct cgtgaaagca
     6601 agacgctact aaacaaatga aaactgccga aagccgtctt ttgagcagct ttcatttggt
     6661 tagtaggagt ccatctagct tgatcacgga attttactta attgatatac gagtaaaaaa
     6721 agtgagattc atgtgaatcc cactcttttt gactaaaatt aacgacgcaa gcctaaacgt
     6781 ttgatcaatt cgcggtaacg ttgaacgtca gttttacgta agtaagctaa caagttacgg
     6841 cggtgaccaa cttttttcat taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt
     6901 gcatgttcat tcaagtggtt gatttcttca gttaatacag cgatttgtac ttctggagaa
     6961 ccagtatccc cttcatggcg agcgtattcg ttgatgattt cattcttgcg ttcttttgaa
     7021 attgccattt ctttcacctc tttcgattat tcccctgcga ctgagtaaaa cgttggtgat
     7081 tcgcttaacc aagtggcagg tcatgtgcct agagcacaca gctatacttt acctgaaacc
     7141 gggttgaaaa tcaagtagtt aatcattaag aaaaggtagt tttcaaacaa tcttactact
     7201 acttttccat ttttgtggta aactataatg cgaaaataga cttgaaacac agtcttttaa
     7261 gaaaggggta catattccat gattacaatg gatgatatca ttcgtgaagg aaatccgacc
     7321 ttacgcgaag ttgcaaaaga agtttctctt ccattaagcg aagaagatat ctctttagga
     7381 aaagagatgc ttgagttctt aaaaaacagc caagatccaa tcaaagctga agaactacac
     7441 ttacgaggcg gtgttggctt agctgctcct caattagata tttctaaaag aatcattgct
     7501 gttcacgtac caagtcctga cccagaggca gatggacctt ctctaagtac tgttatgtat
     7561 aatcctaaaa ttttgagcca ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt
     7621 ttatctgttg atcgagaagt accaggatat gttgttcgcc atgccaaaat cactgtgtct
     7681 tattatgata tgaatggtga aaaacacaag attcgtttga agaattatga atcgatcgtc
     7741 gttcaacatg aaatcgacca tatcaacggt gtgatgttct atgaccacat caacgatcaa
     7801 aatccatttg cactaaaaga aggcgtattg gtgatcgagt agagaattgg caggttcctt
     7861 cgatactatg ctacaagaaa gctttataaa aaaggggagt gctcaaaagc tgcacttccc
     7921 ttttttatgt ttctccacta atcgaaattt agatagacat ctagaaatcg caattccaaa
     7981 aaattctgtg tattggatac gcatcttttc ttattttttc ttatttcttc catatgggtg
     8041 tttatctcag agatgataga atagtttcta ggataaaaag tagcagctac gatgttatcc
     8101 ttcgtagagg attcctgctt cagctagttt ttgtttgatg tttttcagca tatgcttttc
     8161 tgggtagcgg atcgtatgca gatggatgcc gcctgttaat tcggataaca aagtagcatt
     8221 acttttttga taacgtctca taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt
     8281 tcctgctaac tcaccgtaaa ggggatgttc cacaattaca tcaatgattt caccaccatt
     8341 ttcaacgatt agtctcagtt cttcttctgt ttgatctttt ccatgctgga cagcgatttt
     8401 actgatgtgc ccagtattgg tttcttctgt ttcgaggata taacctcttg ctgttgccac
     8461 gatactttta cctgcagctc gtagtaaagc aatatcgcct acaatgatct gacggctgac
     8521 tccaaaagtt ttcgcaaact tgctagcact cactggtttt tctgtatttg ccaaggtttg
     8581 catgatttgt tttcgccgtg tctctccttc gatcacactt attcctcctt cattttgttt
     8641 tggtattcgc gcgagtgttt catcaaatca acatacgtat ctgttattgt ttccttatat
     8701 cccttatttt tcagcagact aacaacttta tccaatactt gctgctctct ttttgcatca
     8761 taaactgctt ggttttcttc ttttttcact tgtcctattt tagttaccgt atccattctt
     8821 ttttctaata aagcaaccaa ctctcgatcg atcatatcga tcttatgacg taatgtttca
     8881 agagactctt ctgttagttc ttcctctact gccttgatga tacgagcagg atttcctgca
     8941 atcactacat tgtcgggaaa agatttggtc acaaccgaac ctgcgccgac tactacgttg
     9001 tcccctagag tgacacccgg cgtgacgata actcctccgc ctaaccaaac attattaccg
     9061 attttaatgg gttttgcgta ctccagaccg ctgttacgtt tcactggatg tagtggatgc
     9121 gtcgccgtgt aaagctggac attcggagcg aacatgcagt tatcaccgat ttcaatcgtg
     9181 ctgacatcta aaaatgtaca attgaaattt gcataaaaat tctccccaac aaatatatta
     9241 tatccgtaat caaaattgat aactggctcc atatagattt ttttccctgt acggccaaaa
     9301 gtctctttca acaattggga tcgcagttcc gctgattctg cttggttgat gatttggctt
     9361 tgacttcttg caaacatacg gtcggcaacc agctcaggat cattggcaaa ataaagctcg
     9421 cctgcaatca ttttttcttt ttcggttttg aattcagtca taatctgctc ctaattcttg
     9481 tatttttttg aaaacaaccg ctccgtcttc ttgaataaac gtataaggct ccgctaaata
     9541 aacaggcgtt tgattgatta ctaagatttt ggctgacggc tggcgaaact gtaccagatc
     9601 acaaaacgga tgaacttgaa aactcgttcc aacaatgacg atcagctctg cttggctgac
     9661 ggcataagcc gcctgctcca tgactccatc ttgaaaccct tcaccatata acacgatgtc
     9721 tgggcgtatt tgtcctccgc atgcttgatg tttgtcgctg tgaagatatt ctttccagtc
     9781 taccgtttgt tgacattttc gacaatgaca ttgatacaaa cttccatgaa aattgacgag
     9841 ttgcgggctt ttagcctttg cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt
     9901 tttttctttc gctagatgtg ccatttccaa atgaatgata ttgggttttg cttcaggatg
     9961 ataaatcttt ttgacaaatt gatagaattt atccggttca ttcaccattg cttgatgact
    10021 cagcaagtat tccggctgct ccatcccgtg gtagattcct gacaaagatc gatagtctgg
    10081 cactccagaa ggtgtggata cccctgcacc ggtcaaaaat gtaatttgct gataagctaa
    10141 tatctctttt gctgcttcgt ctactgttaa ttgtttcatg ttttgtcacc tcactttcca
    10201 ttatagccca tactcgaaca aatttattgt tgaaaagtca gaaaaatggg aataaaaaag
    10261 tgttatgggc atttgaacaa tttatcacaa tgttcactga caaaagaatg aaaacgtgtt
    10321 acaatactgc tgtataaaag tgaggtgaat cgatatgtta ccattcttta tttattgggg
    10381 cgttattctg gcttgtatcg cctggttagc gttaagcgtt tatttctctg ttttttatct
    10441 tgtacgcaaa gaaaatggaa atctttgggc ttttgcttta ttcaacgtga ttgccgcaat
    10501 tgttttagcc attacattag ccgtatatcg aacatggggc tggggaatca cccaatactc
    10561 aagtttgatt tatctaatat tagccattta tggcgtcgtt gttcttttgc aagcgatcct
    10621 aggacgtgag ccaaagaaag cggcagctta aatgatcgag atggaactgg acacaggaaa
    10681 ttttctgttc tagttctttt tttattgtat tacatacatt tttgtgtata attatactat
    10741 cgtttgcaat gaatagatga ttcacatttt gattgttttt tcggtaaaag taaagatttc
    10801 tatatttatc agtatttttt caaaaaacag cagttataga ttgacaaagc ctctcgagta
    10861 tcgtaaactt tttttgtata aataactgaa tacgagatgc ctcaatcaat gaggtagagg
    10921 tcgcgagaat cattaaccct gtggagtgaa gcaaacatgg agaagcagga atagggagga
    10981 tcgccgaaat gtgcactttt ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc
    11041 ggactgtctt agcagtgatg ctaagttgcg ctatgttttg tgagaggttt atcaccttat
    11101 ggaattttag cggccttttt atatctataa atcggtcgct ttttattttt tcagataaag
    11161 ctttaccgca tttcatctag accgctcgtg tatcatataa tcagaaaggg agaaaaaaat
    11221 gaaaaaaaat atggtttcag ttgccgtact atttgctttt gtcttctcac tattcagtgt
    11281 gggatctctg gcaaatgcag atgaaaaaac accgagcgat gagtttcgcg taggtatgga
    11341 ggctgggtat gcacccttca actggtctca acaaacagat gagaataatg ccttgccaat
    11401 tcaaggccaa aatagttacg ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga
    11461 tggtttaggg aaaaaattag tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct
    11521 tcaatcaggc aagatcgacg cgatcatcgc cggaatgagc cctactgctg aacgacgaaa
    11581 agaaattgat tttaccgatc cttattacga atcacaatta gtagtagtag ttcaaaaaga
    11641 tagcaaatat gcaaaagcaa aaagtttagc agatctttct ggagcaaaga ttactgctca
    11701 gttaaataca ttccactata ctgtgatcga tcagatacca gatgtgcaga aacaacaagc
    11761 aatggataat ttctcagcaa tgagaacagc tttagcttct ggaatgatcg atggttatgt
    11821 cagcgaacgt ccggaaggtg taacggcaac tagtgtgaac aaagacttgg aaatgcttga
    11881 attcagcggt gcagatggct tccagacaaa cccggaagat gttcagattt ctgttggtat
    11941 gcgtaaaggc gacccggata tccaaaaagt aaaccagatt ctttctggca tctctaacga
    12001 tgaacgaacc aaaattatgg accaagcagt caaagatcag cctgctgcaa ctgatagtga
    12061 agaaaaacct ggcatcattt ctgatttcac tacgatttgg aaacaatacg gcaatatgtt
    12121 cttacgaggt gctggactta ccttgttcat cgcattgatc gggacagttg tcggtactac
    12181 acttggatta ctgattggtg tcttccgttc ggtacctgaa tcaggaaatg cactgactcg
    12241 cttcttccaa aaaatcggaa atgcacttct ttctatctat gtcgaagtat tccgcggaac
    12301 accgatgatg gtacaagcaa tggtcatctt ctacggtctg gcactcgctt ttggcatttc
    12361 attgaaccga acgattgccg ctttgtttat tgtctctctc aatactggag cttacatgac
    12421 agagatcgtt cgtgggggga tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc
    12481 cattggtatg acccatggcc aaacgatgag aaaagttgtt attccacaag ttttacggaa
    12541 tatcttgcca gcaacaggaa atgagtttgt tatcaatatc aaagatacag cagttttaag
    12601 tgtaatcggc gtagctgatt tgttcttcca aggaaactct gcttccggtg cgaatttcca
    12661 attcttccaa acatttacga tcgtcgggat catctactta gtcatgacct ttacgatcac
    12721 cagaatcctg cgggcggtag aaaagaaaat cgatggtcct tcagcttata ccaagcttga
    12781 agaaatcgac aattcaaatt tgcaggaata aggaggaaga atcatgaatg aaaaaattat
    12841 tgaaatcaaa catctaagaa aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt
    12901 aacagtcaat aaaggtgaag ttgtgacgat catcggttct tccggttctg gtaaatctac
    12961 tttcttgcgc tgtatcaatt tactggaaaa accaacaggc ggcgagatca tttataatgg
    13021 agaaaatgta ttagctccta aatataattt acctaaatat cgaacgaatc taggtatggt
    13081 tttccaatcc ttcaacctgt tcaacaatat gaatgtcttg gaaaactgta tgtctggtca
    13141 aacaactgtc ctgaaacgag ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga
    13201 aaaagttggt atggatcgtt atatcgaagc caaaccagca caattatctg gtggacagaa
    13261 acagcgtgtc gcaattgctc gggcattatc aatgaatcca gatgtattgc tattcgacga
    13321 acctacttcg gcgttagacc ctgaaatggt tggcgaagtg cttaatacga tcaaagactt
    13381 agcacatact ggcttgacaa tgcttatcgt gacgcatgag atggaatttg caaaagatgt
    13441 atctgaccgt gtcattttta tggataaagg tgtgattgca gaagaaggta caccagaaga
    13501 tatctttgtc catccgaaag aagaacgtac aaaagaattc ctttcacgta ttttaaatgc
    13561 ttaggatcaa tagctgattg ggacaaagag ctgcggtgtt taccgcagtt cttttttatg
    13621 aattactcat aaaaggtaaa tttctgaaaa ttttggaatc ttagttgtgt ttttcagaaa
    13681 actcttttaa tatggaagta attaaacttt tttagttagg atgatttaaa aatgaatgaa
    13741 gagcaagaac tgcagcgtgg actgaaaaat agacatgtcc aactgatttc tattggcgga
    13801 gcgattggaa cggggttgtt tttaggttcc ggaaagtcta tccagctagc tggcccttcg
    13861 atattgctcg cctatcttat tactggatgc atctgttttt tcattatgcg tgcgttaggc
    13921 gaacttctgt tatcaaacac caataaccat tcttttctgg attttgttgc agaatatctt
    13981 ggcaaaaaag cggctttcat cactggctgg acgtattggt tctgctgggt atcgattgca
    14041 atggccgatt taacagccat cggaatgtat gtacgctact gggcgcctgg tgttcctcag
    14101 tggcttcctg aactgattgc attaggtctt cttctttgct tgaatatggt ggctgtctca
    14161 ttgtttggtg agttagagtt ttggtttgca ttgatcaaag ttgtagcgat cattgccttt
    14221 atcctcgtag gagcttatat gatcctcacc cactatacga cagatattgg cacagccagt
    14281 atcacgaatc tatggtctca tggcggattc tttccaactg gtgcaaaagg atttatctta
    14341 gctttccaaa tggtaacctt tgcttttgct gggatagaat tagttggatt agtcgctgga
    14401 gaaacagaaa atcctgaaaa agtgttgccg gaagctatca ataatattcc tatcaggatc
    14461 atcttatttt acttaggatc tctttttgtc attatggcaa tctatccatg gaacagcttg
    14521 aatccagata acagtccatt tgtagaagta ttttcggaaa ttgggatcac gattgctgct
    14581 tccttgatca atttagtcgt attaagtgca gcagcttctg cctgcaacag tgcaatatat
    14641 agtacaggac gtatgcttcg ttctttagcg caagaaggca gtgcccctaa aaaattcaaa
    14701 aagttaacaa cacatcatgt gccagggaat gctttgacat tttctactat cgtcattttc
    14761 atttctgtta tcttgaatta cgtgatgcct agtgaagtat tcacactagt aagcagtatt
    14821 gccactactt gtttcttgtt tatctggagt atgcttgtct acacgcacat gaaatatcgt
    14881 aaaagtatcc tcggcaaaaa agctcattca tttaaaatgc cgttgtaccc atttagtaat
    14941 tatctggtat ttctttacat ggcctttgtg tgtgtcgtct tgttcctagg aaaagatacg
    15001 agaatcgctt tattactaac acctgtgtgg ttcttgcttc tattactaat ctatcatatg
    15061 aaatatgaac gtaagaagta agaaagtagt tgtgacagaa gtggtcagct tcgagaaata
    15121 agaaggaatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt atttcttgga
    15181 gttaaaagct tctgtcccag cctcttttct atttccccgg aaacaaccgc tgataatagg
    15241 catctgcttt tttataggaa aaaatccaaa taccagatac aaaaaaacgt ccgaataacc
    15301 cgatcaacgg tacaaaaaag acaccggcaa tcaagcataa gttaatcatc atggagataa
    15361 gacttcgacg gtcctttgac aaaactctca cgacatggga atctggttta tttgctttca
    15421 tgatttgcct catcaagata ccaaaagaaa aactccacat cgtgtatact aagacataag
    15481 ccatctcagg ccaaaaagct tcagggttac gcccgaccca actagtaaca gaaggacata
    15541 aagtcagcca aaagagcaaa aagatattcg accagaccac tcggtagtta attcgttctg
    15601 ccatctgaaa tatacgatga tggttcaccc agatcaccgc aacaaatata aagctgatgg
    15661 tataagctaa taatgtattt cggatgaccc ataaagaatg aaagctagat gtattaggga
    15721 tcttgatctc taatacaaga actctcaaaa caatggctac cactccatca gtaaatgctt
    15781 ctaatctcga ctttgtcacg atgattcctc ctcagatgat tcagcgcttt tattttatca
    15841 cttacgttat t
//
LOCUS       DAXWLF010000050        15847 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXWLF010000050 DAXWLF010000000
VERSION     DAXWLF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.050"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            25..459
                     /locus_tag="ACGE4U_001244"
     CDS             25..459
                     /locus_tag="ACGE4U_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFF8393420.1"
                     /translation="MSVKNPTQETIFNYLEETKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            625..2670
                     /gene="parE"
                     /locus_tag="ACGE4U_001245"
     CDS             625..2670
                     /gene="parE"
                     /locus_tag="ACGE4U_001245"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFF8393421.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            2682..5132
                     /gene="parC"
                     /locus_tag="ACGE4U_001246"
     CDS             2682..5132
                     /gene="parC"
                     /locus_tag="ACGE4U_001246"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFF8393422.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            5409..7640
                     /gene="pflB"
                     /locus_tag="ACGE4U_001247"
     CDS             5409..7640
                     /gene="pflB"
                     /locus_tag="ACGE4U_001247"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFF8393423.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            7884..8645
                     /gene="pflA"
                     /locus_tag="ACGE4U_001248"
     CDS             7884..8645
                     /gene="pflA"
                     /locus_tag="ACGE4U_001248"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFF8393424.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            8730..9656
                     /locus_tag="ACGE4U_001249"
     CDS             8730..9656
                     /locus_tag="ACGE4U_001249"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFF8393425.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            9838..10731
                     /locus_tag="ACGE4U_001250"
     CDS             9838..10731
                     /locus_tag="ACGE4U_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFF8393426.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSYFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            10865..10990
                     /locus_tag="ACGE4U_001251"
     CDS             10865..10990
                     /locus_tag="ACGE4U_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFF8393427.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11124..11756)
                     /locus_tag="ACGE4U_001252"
     CDS             complement(11124..11756)
                     /locus_tag="ACGE4U_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393428.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(11904..12446)
                     /locus_tag="ACGE4U_001253"
     CDS             complement(11904..12446)
                     /locus_tag="ACGE4U_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFF8393429.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            12581..12970
                     /locus_tag="ACGE4U_001254"
     CDS             12581..12970
                     /locus_tag="ACGE4U_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393430.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12974..13738
                     /locus_tag="ACGE4U_001255"
     CDS             12974..13738
                     /locus_tag="ACGE4U_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8393431.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            13867..14814
                     /locus_tag="ACGE4U_001256"
     CDS             13867..14814
                     /locus_tag="ACGE4U_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8393432.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            14944..15684
                     /locus_tag="ACGE4U_001257"
     CDS             14944..15684
                     /locus_tag="ACGE4U_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393433.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         5584 a         2663 c         3315 g         4285 t
ORIGIN      
        1 aaagaaaagc aaggggtggc agaaatgtct gtaaaaaatc caacacaaga aacgattttc
       61 aactatttgg aagaaacaaa aaatattgcc gtcgtaggct tgagcaataa accagaacga
      121 acaagttaca tggttgcaca ggtattgcaa gacaatggat ataaaatcat accagtaaat
      181 ccattattgg ctggtgaaga aattctaggc gagaaagtct atgcgaattt gacagaaatc
      241 gatgatccaa tcgatatcgt agatgtattt agacggagtg aatttttacc tgaaatagca
      301 ggtgattttt tgaaaactga tgctaaagtg ttctgggcac aattagggat tgaaaacaaa
      361 gaagcagctg agatgctaag aaaagcagga cgtacagata tcgtaatgga tcgttgtatt
      421 aagattgaac ttggtaagtt gaaagaaaac caaaaataaa aatgaccaca aagaagagat
      481 gatcgataga ggggaatgtc gaaaaagcgg ttaggagata attaaaattg atttctcgtc
      541 taccctcttt tttttcacga ccttttctag tatgataatc gtggtgcggt tttgccgcaa
      601 agtattattg aaggagcgtt tgccttggca aaaaaagtaa acaacgaata caatgactcg
      661 tccatccaag tattggaagg attagaggca gttagaaaaa gaccagggat gtacattgga
      721 tccactgaca gccgaggctt gcatcattta gtctatgaaa tcgtcgataa tgcagttgac
      781 gaagcattat caggttatgg cagtgagatt gatgtgacca tccatgaaga caatagtata
      841 actgttgcag atagcggacg tgggatgcct gtaggaatgc atgcttccgg aataccaaca
      901 gttgaagtga tttttactgt gctgcatgct ggcggaaagt ttggtcaagg agggtacaaa
      961 acttctggtg gactgcacgg tgtaggagcc agtgtggtaa atgccttatc gaaatggcta
     1021 attgtaacga tcgttagaga tggcgtagag tatcagcaga aatttaaaaa cggcggtaaa
     1081 cctgacggta ctttgaaaaa gataggaaaa acaaaaaaag caaacgggac gactgtccat
     1141 tttttaccag atgatacaat attcagtaca acgaaatttt catatgaaat tttagccgaa
     1201 cggttaagag aatctgcctt tcttctaaaa ggcgttaaaa tctctctttc tgatcttcgt
     1261 ggagaagaac cagtcaagga aatttttcat tacgaagaag gaatcaaaga atttgttgat
     1321 tatctgaatg aagaaaaaga tacattgaca ccagttgttt atttttcagg agaaaaagaa
     1381 ggtatcgaag tagaggtcgc ttatcaatac aatgacggtt attcagaaaa tgttttatct
     1441 tttgtcaata acgttcggac aaaagatggg ggaacccatg aagctggtat gaaagcggcg
     1501 atgacgaaat cttataatga atatgcgaga aaagttggtt tgctgaaaga acgggacaag
     1561 aatctggaag gtagtgattt cagagaaggg ttagctgctg tactttctat ccgtgtacct
     1621 gaaaatcttc tgcaatttga agggcaaaca aaagaaaaac ttggtacgcc agttgctcgt
     1681 accgtagtag ataacgtcat cagtgaacaa atgggctttt atttgcagga aaatagcgaa
     1741 atgagccaga tgttggtccg taaagcaatc aaagccagag aagcgagaga agcagcacgt
     1801 aaagccagag aagaaagccg aaatgggaag aagcgtaaaa aaggcgaatc tcttctttca
     1861 ggaaaattga cacccgcaca atcccgaaac cctaagaaaa atgaattgta tttagtcgaa
     1921 ggggattctg ctggcggatc tgctaaacaa ggaagagaca gaaagttcca agcaattcta
     1981 cctttacgag gaaaagtcat caataccgaa aaagctaaaa tgcaagatat cttgaaaaat
     2041 gaagaaatca atacgatgat ctatacgatt ggtgcaggag tcggtcctga gttttctatc
     2101 gaagactgta attacgacaa agtaatcatc atgaccgatg cggatacaga cggcgcccat
     2161 attcaagtat tactgcttac tttcttttat cgttatatga aaccgctgat tgaagctggg
     2221 aaagtatata tcgcgttacc gcctttatat aaagtatcca aaggacaagg aaagaagcaa
     2281 gtcattgaat atgcgtggac agatgacgaa ttggcagcaa tgattaaaaa agtcggcaaa
     2341 ggatatatgc tgcagcgata taaaggactt ggagagatga atgcggaaca attgtgggaa
     2401 acaacgatgg atccgacttc ccgtacgttg atccgcgtcc gtatcgatga tgctgcgcaa
     2461 gctgaacgtc gagtaaccac actgatgggt gacaaagtag aacctcgaag aaaatggatc
     2521 gaaaatcacg tgcaatttac gctcgaagaa gatggcagta tcttagataa aaaagaggat
     2581 actgaaatct ctccttcagt ttctaatgat ttattagatg aagaacgagc agataaaaat
     2641 gaaaacaatc agttatttga agtagaatag gaggtcacct gatggaaaat cgacaagaag
     2701 tacaagaatt aactttagaa gaagtgatgg gtgaccgctt cggacgttat tccaaatata
     2761 ttatccaaga acgtgcactg cctgatatcc gtgatggatt gaagccagtg caacgccgga
     2821 tattatttgc catgaacaaa gatggcaata cgtatgacaa aggattccgt aaatcagcaa
     2881 aatctgtcgg aaatatcatg gggaattatc atcctcatgg tgacatcagt atttatgaag
     2941 cgatggtccg catgagccaa gactggaagt tgcgggaagt actgatcgaa atgcacggga
     3001 ataacgggag tatggacggt gatccacctg ctgcgatgcg ttatactgaa gccagattat
     3061 ccaagttaag tggagagatg ctagcagata tcgagaaaga aacagtcgat ctcgtgtgga
     3121 attttgatga tacagaaaaa gaaccaacag tccttccagc tcgataccca aaccttctcg
     3181 tcaatggatc gacaggtata tctgctggat atgcaacaga aatcccgact cataacttag
     3241 ctgaagtgat cgatggcaca atctatatga tcgatcatcc acaagcgagc cttgaaaaat
     3301 tgatggaata tattcctgga cctgactttc caacaggagg gatcttgcaa ggaaaagatg
     3361 aaatcaaaaa agcttatgaa acaggacgag gaaaagttat tcttcgttcg aaaacaaaga
     3421 tagaggcaat gaagggtaat aaacagcaaa tcgtgatcac agaaatccca tatgaagtaa
     3481 ataaagcgac cttggtgaaa aaaatggatg agatccgttt gaataagaaa atcgatggaa
     3541 tagctgaagt acgagatgaa agtgatagaa caggtctgca aattgtcgtc gaattgaaaa
     3601 aagacgccaa tgctcaagga attttgaatt atttgttcaa aaacactgag ctacagatca
     3661 attacaattt caatatggtc gctatcgacc atatgactcc gcatcaagtt ggactgaaag
     3721 acattttaag aagttatatc gagcatcgaa agcaagtcat aacaaaacgt agtcaattcg
     3781 atttagccaa agctcaaaaa agacagcata tcgtagaagg attgatgaaa gctttatcga
     3841 ttctggatga agtaatcgca acgattcgtg aaagtaaaga caaaaaagac gccaaaaaga
     3901 atttagtcga cgtctttcaa tttacagaag aacaagcaga agcaattgtc acattgcaat
     3961 tataccgttt gacgaacacg gatatcacag agcttcaaaa ggaatccgaa agcttgattg
     4021 cgcaaatcac agagttgaat aaaatccttt caaatgacaa agagcttttc tctgttatga
     4081 aaaaagaatt gcgagaagtg aaaaaaaatt attcttccgc tcgtctaacg acaatcgaag
     4141 acgaaatcga agaaatcaaa attgatacgc aagtcttagt ggcacaagaa gacgtaattg
     4201 tcagtgtgac gcgagaaggc tatgtgaagc gaacaagcct tcgttcttat agtgcttcca
     4261 aaccagaaga aatcgggatg cgagaaggcg actacttgct atactcaggt gaattgagta
     4321 cattggacca tgttcttctg atcacaaata aagcaaatgt gatctaccgc cctgtccatg
     4381 aactacccga tttgaaatgg aaagatgctg gtgagcacat ttctcaaaca atctccaatc
     4441 tgtctgtaga cgaatcgatt ttagctgttt tcccatatca aaaaattcaa gcagagaaaa
     4501 catttgtatt catcagcaag aatggattga tcaagcaaac acgaatgaca gagttcgaac
     4561 catggcgcac ctataagagc cgtccgctca gctgtatgaa gctaaaggcg gaagaagatg
     4621 aactgattgc tgtgtatctg aaagaagatc agccaggctt agatgttttc ttagtaacgc
     4681 atcaaggaat gggtttgcgc tatcctttaa cagatgttcc cgttgttgga gcaaaggcag
     4741 ctggagtgaa gtctatgaat ttgaaagatg gagataggat tatcaacggg ctgcttgtgt
     4801 tagcagaagg tgatacgccg atcgtaatcg ttactcagcg aggagctgtt aaacgcatgc
     4861 ttgctcaaga aatcagccaa acttcacgtg cgaagcgggg agtgacggtc atgcgtgagc
     4921 tgaaaaaaaa tccgcaccgt attatctata tgtcggaagg aagatcaaaa gaaataacct
     4981 tgatgaatca aaaagggcag cagttaacga ttgacccaac tgatttccct ataggtgatc
     5041 gaacatcgaa tggatcattt gcgatggatg agaaaaaagg tggagaagtc atcgaagtta
     5101 tcgatgctcc tgagattttc atcgagtcgt aactgttgta gtattttaat gaagagacgc
     5161 catctgtatt aatacaggtg gcgtctcttt ttcattcatt tttaataaat ccttatacgt
     5221 ttgcaaaaaa agatgaaagt gttcacaatg cttaaatgtt ggtatgtcaa cgttgattta
     5281 aatatatgta aataaaacat aaaaattgtt aaataaaaaa caagaaaacc cttgcaaaga
     5341 tagggtgctg tgttatacta atcacgtaaa aactgttaca taaaacagaa atttaggagg
     5401 tccatacaat ggagcaatgg aatggattta aaggtaccaa atggaaagaa ggcgtagaca
     5461 ctagagactt tattcaaaat aactacactg aatatcgcgg tgatgacagc tttttggaag
     5521 gcccaacaga agcaacagaa aaattatggg atgaattgca aaagttaaat gatatccaat
     5581 tcgagcgtaa cggtgtctat gatatggaca cagaaatcgt atcaactatt acttcacacg
     5641 atgcaggcta tttggataaa gatttggaaa aaatcgtcgg tctacagaca gacaaacctt
     5701 tgaaacaagc attcatgccg ttcggcggaa tcaatatggc taacaatgcg ctaaaaagca
     5761 atggctatac accaaacgat gaattgacac atattttcac tgactggcgc aaaacacata
     5821 atcaaggtgt atttgacgct tatactgctg aaatgcgtgc tgctcgtaaa aacaaaatca
     5881 ttacagggct tccagacgct tacggtcgcg gacgtatcat cggtgactat cgtcgtgtgg
     5941 cattgtatgg aatcgactat ttgatggaac aaaagaaaaa agatcatgat atgactggtt
     6001 atcatacaat gagtgaagaa gtgattcgtt tacgtgaaga aatcactgaa caatatcgtg
     6061 cattagcgca aatgaaagaa atggctgcga aatatggcta tgatatttct aaaccagctg
     6121 caaatgcaaa agaagctgtc caatggctat acttcggata tttagcagca atcaaatcac
     6181 aaaatggtgc tgcgatgtca atcggacgta tctcagcatt ccttgatatc tatatccaac
     6241 gtgatttgga taatggagta atcacagaaa aagaagcaca agaattgatc gaccacttag
     6301 taatgaaatt acgtatggtc aaattcgcgc gtacacctga atataaccaa ttattctcag
     6361 gattccctat ctgggctact ttatccattg caggtatggg attagatggt cgttcattag
     6421 ttacaaagaa tgatttccgt ctgcttcata cattaacaaa tatgggacct tcaccagaac
     6481 caaacttgac tgttttgtat tctgaacacc ttccagaagg attcagaaca tatgctagca
     6541 gaatcgctat cgaaagttct tcgattcaat tcgaaaatga taatcagctt cgtgaacaat
     6601 ggggttctga tgattgtgcg attgcttgct gtgtatcagg aacagttgtc ggaaaagaca
     6661 tgcaattctt tggcgctcgt gctaacttgg ctaaaacact attatatgcc atcaatggcg
     6721 gagtcgatga agtgactggc gcccaagttg gaccagaata tcgtccaatc acatcagaaa
     6781 tactagactt tgaagaagtg aaacataact tcatgaatat gatggattgg ttagcagaac
     6841 tatatgtcaa tacgttgaac atcattcatt atatgcatga taaatattct tatgaagcag
     6901 ctcagctggc tttgatggat tctgacttgc aacgcacatt tgctacaggt attgctggga
     6961 tctctcatgc ggcagacagc ttgtcagcga tcaaatatgc aaaagtaaaa gcaatccgta
     7021 atgaaaaagg aatagcagtt gattttgaag tggatggcga cttccctaaa tacggaaatg
     7081 atgatgaccg tgcagatgaa ttagtttcat ggatcttgga atacttcatg ggacaaatca
     7141 aacgtcataa aacttaccgc aactcaaaac caacaacttc attattgacg attacttcaa
     7201 atgttgttta cggtaaaaat acaggaaata ctcctgatgg ccgtgaagct ggaaaaccac
     7261 ttgcgccagg agcaaatcca agttacaatg cggaacaaag tgggttatta gctagcctga
     7321 actcaacagc taaaatgaat tacggctgtg cattggatgg aatttcaaat acccaaacaa
     7381 tcaatccttc aggtcttgga aaagaacaag atacaagaat cgaaaacttg cgtaatgtct
     7441 tagatggata cttctcaaaa ggagcttatc atttgaatgt caatgtattc agtaatgatt
     7501 tattgcgtga tgcaatggaa aatccaatga aatatccgaa cttgacgatc cgggtatctg
     7561 gttatgcagt taaattccgt gacttgacaa gagaacaaca attagatgtc ttgatgagaa
     7621 cttcacacga cagaatgtaa aaagttcgtg aaaaaagttt ttcgacttag cgtcgaaagg
     7681 acgttgaata gccatcgcat caaaatcgaa taatcagtat gcttatttga aagaaacgat
     7741 ttacaggggc aggacttgta tgttccgtcc ctgtaaattt tacacataac gaagcggcaa
     7801 ttgcgaaaaa ttttgttcag aaagcttcgc ttcgacagct aacttgtact taaaatgaaa
     7861 tcaaagaaaa gatgtgaact taaatggaag aaaaaacaat cggctatgtt cattctatcg
     7921 aaactttcgg ttcagtagac ggacctggac tgcgctttgt cgtgtttatg caaggatgcc
     7981 gcatgcgttg ccaattttgt cacaatccag atacttggaa tattggcgga gggaaagaat
     8041 acacagcaga tgaactttta gataaagcag aacgcttcag accttactgg ggagataaag
     8101 gtggtattac tgttagtggt ggagaacctc tattgcagat cgattttttg atcgaactat
     8161 ttaaaaaagc aaaagagcgg aagatgcata ctacattgga tacatgcgga aaacctttta
     8221 cgtatgaaga acctttcttt tcacgttttc aagaattgat gaagtataca gatttactgt
     8281 tatttgacat caaacacatc gataatgaag agcataaaaa actaacgcat tgggacaatg
     8341 aaaatatttt agaaatggct caatatttgt caaaaatcaa caaaccggtt tggattcgcc
     8401 atgtactagt tcctgagcga agtgattatg atgaatattt gatccgacta gacaacttta
     8461 ttcaaacctt gtcaaatgta gacagagtag agattcttcc ttatcatacg atgggaaaat
     8521 acaagtggga aacacttggt ttgaaatatc cattagaagg gatcgaacca cctacaaaag
     8581 aacgtgtcga aaatgctaaa cgattgcttc atacagaaga gtatacaaaa tatttaacgc
     8641 gataggtcgc atcggaaaga agataagtat tgcttctttc cgtttttttg ctattattaa
     8701 ataggagaaa tcacaaagga gcgaatgaca tgtcaaaaat tttagttttt ggtcaccaaa
     8761 atccagatac agatgcaatc ggggcagcca ttgcgttttc acacttgcaa aatgaattag
     8821 gtaaagaaac agaagcagtt gctctaggta cacctaatga agaaacacaa tacgcattag
     8881 accactttca gctatctgca ccacgagtag tagagtcagc taaagcagaa aatgcagatg
     8941 tcatgcttgt ggatcataat gaattccaac aaagtatttc agatatcagc gaagtaaata
     9001 ttttagctgt agtagaccac catcgtattg caaactttga aacagctaat ccattgtact
     9061 atcgtgcaga accagttgga tgtacaagta cgattatttt aaaactatat aaagaaaacc
     9121 aagtaacaat tcctaaagaa atcgcaggaa tcatgctctc agctatcatt tcagatacat
     9181 tgttgttcaa atcaccaaca tgtacgcctg aagatgaagc agctgcaaaa gaacttgcag
     9241 caatcgctga agtcgatcta aacacttatg gacttgaaat gttgaaagca ggaacaaatc
     9301 taagtgataa atcagctgcg gttcttcttg atttggatgc aaaaagtttc ccaatgggca
     9361 gttccaacgt tcgtatcgct caagtcaata cagtggacct taatgaagtg atggatcgac
     9421 aagcagagct agaagcagca atggaagatg aaatgaaagc aaatggctac gatctgttcg
     9481 tattagttgt cacaaacatc ttagacagtg attcagaatt acttgttgct ggtaaaggaa
     9541 aagaaaaagt agaagaagct ttccaaacaa ctttgaccaa caaccgtgct ttgcttaaag
     9601 gtgtcgtttc acgtaaaaaa caagtcgtac ctcaattgac ggctgtattt agctaatcaa
     9661 atggtagtat gaagcattca aaatgaatgg ataagcgatt gatataaaaa gaaacataga
     9721 acatactaaa ggccgaagaa atggaggaga ataacagtca attatgactc ttgttcttcc
     9781 tcctttttac gtaaaagcct tttaagtaaa ataaaacgga tagaaaagag aacatatatg
     9841 aactattatt caaaaaataa ggaaaacttt tatcaaaaac tgaccgatga tccgcttttt
     9901 tcttcattga ccgattatct atatgaacat cgagaacaag aaacgattct aagagaattg
     9961 aaaaaagaat tttcgcaaaa taaattttcc tattttcttg atttattgat cgatgcagga
    10021 ttaatcaaga gggaagaacg aagatatcat ttgaactttc caatatttga tcctaaagat
    10081 tacttgcaac aagccacttc ggctgctgaa accatagctg aacaattgaa gagactctct
    10141 gttgatgaac aaaaattagc gatgggagaa gttatttggg cgtattgttt cgaagatgaa
    10201 cggaaagaag cgtattttta tggagtaaga aacagccgag agacagaatt gctgaggaca
    10261 actgcaggaa atgagaagta ccgttttatc acactttcct ctatagaaca cttcccgctt
    10321 actttggcta attacttttt tgtacagaag aatcagcttc ctgtaacgaa ggcattcaaa
    10381 gaattagccg aacttattgg agacgtaaat gaatcgtact tttttgatca aatagaagtt
    10441 atcgttgaca ggatacgtaa gaataaatat aaaaatcgcc ggcctagtat tttccatcag
    10501 tctctacttg tgacgaatac tataaaggaa gaagaatcat tcacattaga attacctatt
    10561 gtcgaaaaaa acaattttga gattgaatta ccaacattgg atccttcatt gacaatggag
    10621 gaaatggcct ttcttaaacg acaaatcttt tctgagctga gcaagaaatt tataccgcat
    10681 gcattttctt atatcaaaga atatggaaca gttttagtga gtaaaaccta agagagcagt
    10741 aagcaaaaat tatactgact cttgcaggag gagcctttct cttcctttat ggttaaaaga
    10801 attatttgag tattttgcct atctaccacc tattagattc actacatggt ctggaaacaa
    10861 aggaatgcaa aaaaatggtg agaaatgtgg aatgacaaag gaagtagtga taagaaaagt
    10921 tagattttta aataatcaat attatgattc agtcaagtat ggcattttat gggaagaact
    10981 agctgattag attaggaagt ccatttgata atccagaatc actaagaaac gaatggcgag
    11041 tcattaataa tttatccaat cttgggttat tcttttagat tgaagtgttt aaaactaaaa
    11101 aagcatttat gaagtcgtaa tagtcaggct tcaaaatgct ttttattttg tttgtttttt
    11161 ctttgccaca taaatacacc tagactgacg atgaagagca tatccaacag atttcctgcg
    11221 acttggatcg tcgaagggtt ggtcagtggc attaaagcaa ggacaaaaaa ggaaaacaag
    11281 ctgtaaataa aacaggtgac aggaactaat cgaaagaaga gcggacattt caattggcta
    11341 aaaatgagtg aaaaaataaa ataagtaaaa atggataaaa tcacgactaa accattcatg
    11401 ataaagaaga aaagcgtatt gtgattaaac gaataaaaaa gaattgctaa aaaagaaaac
    11461 gttttaaatg caatcaatac gcttgaagct tcgtttaacg taccaggatg tcgatgaaaa
    11521 aaataggcac cgaaataaac agaaaacagt aagccgcaaa gagaagacaa aatcaaaaaa
    11581 aacggatttt cttcggcttc tgtgatatca gaatgatcca aaagcaacca gcctaatgca
    11641 ctagaaggcg catgtgaggt cttaaggtat gaataacata gaaaaaataa gatcgtcaaa
    11701 gttgcaaaaa aagagcctaa atgtaaaaaa ttagtggttc tacgttgaat ttccataata
    11761 tctccaaatt aaattgataa gtatataaag tataataaca aattgatgag tatatgtcga
    11821 tgttttttaa tcaaaataat aaataaaaac tgtctaacat ttaaaattta atttagatat
    11881 tagacagttt ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt tttagtccag
    11941 aaaccacctg aaatatattt tggctcataa gaaatgataa aagcacgctc ttcgatttct
    12001 ttgatcaggc gatacagttc acgttcgctt tttctaggag aaagtatttc gagaactagc
    12061 cgctcacctt ccagccccat tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt
    12121 atacccggca aacttttatc ttctgcgcta tttgctggca aaatcgttgt gaccatgatg
    12181 tatcctaatg ccaatttgtc ttcaatcaaa attccgacac tgatacctac tgcgtaacct
    12241 aacgcataga caaataaatt cagtggatta tctaaacgat ctaaaacaag ggataagccg
    12301 acgatataaa tcgtgatctc gaccatactg acaagtggag caatcagtcg atagcctttc
    12361 attgtcaaca tgaaacgtaa cgtgttcaga gtgatataag caaaattaat gaggaagatc
    12421 atccccaaca tttttggatc aatcatttgt aaaactcctt tcatttatat aacaacaata
    12481 tcaaaaaaaa tcgtaagtga agaaaaattg aaactttata catacaaaaa aatgataaaa
    12541 tgattataat agaattataa agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt
    12601 gttttgtgcc agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat
    12661 tcaacagcca attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat
    12721 tttaagcgtt tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag
    12781 ttttttgaat ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg
    12841 ttctctgtgc ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa
    12901 cctgttccag acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat
    12961 aggtgagtaa caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt
    13021 agaagttgtt ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg
    13081 atggcagaca agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat
    13141 tcgtgtgatc ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat
    13201 tccatcctat acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat
    13261 caagcattta gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat
    13321 ggggggaatg acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg
    13381 tttgatgggt tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga
    13441 atataattat gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga
    13501 tttgtcattg cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc
    13561 tgacgaaaaa atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc
    13621 ttacggaaaa gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaatacc
    13681 actaatggaa gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt
    13741 gaaacggaaa atcactcatg acgatagagg attttccgtt ttttattttg ggctataata
    13801 aactctgtta ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa
    13861 gagagcatgc ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc
    13921 tctcgagtca tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca
    13981 gcaatggctg aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg
    14041 tcgcagatca tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg
    14101 aatttgctga tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt
    14161 acaaatgatg ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa
    14221 aaagacttcg attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct
    14281 gaaggatatg attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat
    14341 gccttagaaa gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt
    14401 gaaatttcaa gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa
    14461 atcttgcggt ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca
    14521 gattttacag ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta
    14581 agagggatta taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat
    14641 ggggtatttt tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata
    14701 cgtatgggtg aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt
    14761 caaggttatc gtcgtttttt tcctgaacta gttatcagag agacaacgaa ttaagtctta
    14821 ttactatata tatatttatg aaattcatcg ctattttctt aaaataatca gcgcattgct
    14881 actgaaaaca atgaatctgt gctattattg agaaaagttc catttttgag aaggatggtt
    14941 tgaatgaaag aaatcagacg aaaacatttc cgttttcctg cgtacgatga tgagttaggc
    15001 gtaaaattaa ccaacgacaa gaagcgagta ttatttgacg gacaagaaga tcttttgact
    15061 gaacagaatc agtccccgtt tgaacagatg aaagaatttc atttcacgaa cacgaacacc
    15121 gagaaaatcc gtcgcaagcg tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa
    15181 aatttggaaa agcataaagg aaaattaccg gattataccg gccattacca atcagaaatc
    15241 acatcgacag gacgaaaaaa actttttggt gattcctctc gttctgtcag acaaagtgaa
    15301 acaaggacag agtccaagca tactacacga aacgcatctg ttgacacgaa ggtgcgggag
    15361 cgttcttatt ttgttcctaa atatgtaccc gcatcgatta tcccagaccc tagagaaagc
    15421 aaaatttctg aggatcattt aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg
    15481 ttcgatacag aaccgacgcc gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca
    15541 ttcaatcgaa caaaaacgtt agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa
    15601 agccatggta tcttagaacg ctcattacaa ggattgatcg atgatcaagg aaactctttg
    15661 ggagaaaata gctattttaa ataagtgata tacaaattta tattcggcta cagttggaaa
    15721 ggaaacgctg cggcgtttcc ttattttata ctctatccga aagtttatat cgtatcctca
    15781 aatttttttg agtttaagcg aattttttct cccaataagt aagaaaataa tggcaaaata
    15841 aaaaaac
//
LOCUS       DAXWLF010000051        15804 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXWLF010000051 DAXWLF010000000
VERSION     DAXWLF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.051"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            32..784
                     /locus_tag="ACGE4U_001258"
     CDS             32..784
                     /locus_tag="ACGE4U_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8393434.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            781..1695
                     /gene="pfkB"
                     /locus_tag="ACGE4U_001259"
     CDS             781..1695
                     /gene="pfkB"
                     /locus_tag="ACGE4U_001259"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFF8393435.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1713..3644
                     /locus_tag="ACGE4U_001260"
     CDS             1713..3644
                     /locus_tag="ACGE4U_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFF8393436.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3755..5314)
                     /locus_tag="ACGE4U_001261"
     CDS             complement(3755..5314)
                     /locus_tag="ACGE4U_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFF8393437.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5469..7703
                     /gene="pcrA"
                     /locus_tag="ACGE4U_001262"
     CDS             5469..7703
                     /gene="pcrA"
                     /locus_tag="ACGE4U_001262"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFF8393438.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7720..9756
                     /gene="ligA"
                     /locus_tag="ACGE4U_001263"
     CDS             7720..9756
                     /gene="ligA"
                     /locus_tag="ACGE4U_001263"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFF8393439.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9862..10167
                     /gene="gatC"
                     /locus_tag="ACGE4U_001264"
     CDS             9862..10167
                     /gene="gatC"
                     /locus_tag="ACGE4U_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFF8393440.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10167..11639
                     /gene="gatA"
                     /locus_tag="ACGE4U_001265"
     CDS             10167..11639
                     /gene="gatA"
                     /locus_tag="ACGE4U_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFF8393441.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11636..13066
                     /gene="gatB"
                     /locus_tag="ACGE4U_001266"
     CDS             11636..13066
                     /gene="gatB"
                     /locus_tag="ACGE4U_001266"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFF8393442.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13076..14131
                     /locus_tag="ACGE4U_001267"
     CDS             13076..14131
                     /locus_tag="ACGE4U_001267"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFF8393443.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14242..15615
                     /gene="rlmD"
                     /locus_tag="ACGE4U_001268"
     CDS             14242..15615
                     /gene="rlmD"
                     /locus_tag="ACGE4U_001268"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFF8393444.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15618..15746
                     /locus_tag="ACGE4U_001269"
     CDS             15618..15746
                     /locus_tag="ACGE4U_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFF8393445.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5373 a         2898 c         3470 g         4063 t
ORIGIN      
        1 tgctattatt tgtattgagg tgatcgtgaa aatgcttaca gaggaacgta gacaaaaaat
       61 cctagacttg cttgaacagc agaaaatcgt gaaatcacaa gatcttgtaa acctttttaa
      121 tgcttctgaa tcaacgataa gacgtgattt acaagagctg gagaatagtg ggtttctaga
      181 aagagttcat ggcggagcta agaaagagca attattgaat tttgagcaga atatgagtga
      241 aaaatcactc aaaaacgttc acgaaaaaca gcaaatcgcc aaatcggcag ctgaaaccat
      301 taatgacgaa gatgtcattt atttagacgc tggctctact acgttagaaa tgattccttt
      361 tctgcaagga aaacagatca cagtagtgac aaattccgtt caacatgcgg caaatttagt
      421 tgatttggag cttgctacca ttattttagg tggaacgata aagctggcaa ctaatgctgt
      481 tttaggatct aatgcgatag aacaactaaa aaattttcat tttaataaag catttatggg
      541 aatgaacggt gcccatcttg aacaaggatt cacgactccg gacccagaag aggcagctgt
      601 taaacgttta gccatgaaaa attccgaaga gacatttgta ttgattgatc ataccaaatt
      661 caaccagttg tccttcacct ctgttgcacc tttggaagca gccacgatca taaccgagcg
      721 ttgtccatta gaattcgtta atgaattccg tgaaaagaca acaattaagg aggcaataca
      781 atgatttaca cagtaacact taatccatca attgattata tcgtacgcgt agatgggtta
      841 aaactaggcg atttgaaccg aatgaaggaa gacttcaagt tacctggagg aaaagggatc
      901 aatgtttccc gtattttgaa acggatcgat tctcaatcta cagcactagg gtttttagga
      961 gggttcactg gcgaatttat ttcgaagtgg cttgaaaaag aacaaatcca gtctttgttc
     1021 acaaccgtaa aagaagatac aagaatcaat atcaaactaa aatcagatac tgaaactgaa
     1081 attaacggtt tgggcccatc tatttcagat gaagaaatcg atgagttgaa gcaagcatta
     1141 ggaacagtag gacaaggaga tatcgtcgta ttgtcaggaa gcgcgccggc tagcctgcga
     1201 aaaggatttt atcaagaact gattgatatc attcgagaaa aaggtgcaga tttcgtgatt
     1261 gatacaacag gtgatgacct aaaaaatgca cttgttaaga aaccattgtt gatcaaacca
     1321 aacaatcacg aattagctga gctttacaat acaaccttcc aatctgtaga agaaatcatt
     1381 ccgtatggta aaaaattgct agctgaaggg gcacagaatg tgctgatctc aatggctgga
     1441 gacggtgctt tgctcttcac ggaacaagga acttataaaa cgaatgtatt agttcgccca
     1501 ttgaaaaatt cagtaggtgc aggggattca atgattgccg gctttattgg ggcttacagc
     1561 cgtacgaaag atccaatcga agcctttaaa tggggagttg cttgcggaag cgcaacagct
     1621 ttttctgatg acttggcagc tcgcgaattg attgatgagt tgctgccaga agttgaaatc
     1681 acaaaaattg attagtttca aggaggataa tcatgaatat caaggattta atggtcaaag
     1741 aagcgatgat catggatttg caagcgactg ataaaaaagg cgcaatcgat gaaatggtcc
     1801 aaaaaatgtt tgatgccggc cggatttccg atatcgaaac atataaagaa ggaattctag
     1861 cccgcgaagc acaaacttct acaggtcttg gtgatggaat cgccatgcct cacgcgaaaa
     1921 acagtgcagt taaagaagca acagttttat tcgcaaaaag ccaaaaagga gtagattatg
     1981 aagccttaga cggacagcca acttatctgt ttttcatgat cgcagctcca gaaggcgcaa
     2041 acgacacaca tttgcaagca ttagcagcac tatctcgatt gctgattgat gcagatttcg
     2101 taggaaaatt aaaagaagca aatacaccag aagaagtcca agacttgttc caaacagctg
     2161 aagagcaaaa agaagcagaa gaaaaagcag aacaagaaac acaagctgca gaagcttctg
     2221 ctcatccaga aagaaaattc gttgttgccg ttacagcttg tccaacaggg atcgctcaca
     2281 cttacatggc agaagatgct ctgaagaaaa aagcgaaaga aatgggtgtg gagatcaaag
     2341 tcgaaacgaa cggttcagaa ggaatcaaaa accgattgac tgctgaagac atcgcccgtg
     2401 cagaaggtgt aatcgttgca gcagacaaaa aagtagagat gaatcgcttt gacggaaaag
     2461 aactgatcaa tcgtcctgtc agtgatggta tccgtaaacc agaagagctg atcaatctag
     2521 cccttagtgg tactgcacca gtattccatg gtgatggaaa agaaagcagt tcagaagaag
     2581 gaaatgcgga tggtactatc ggacaaagaa tttacaaaga tttgatgaac ggcgtatcac
     2641 acatgttacc tttcgttatt ggtggaggaa tcgctattgc gctttctttc atgatcgacc
     2701 aatttattgg cgtgccacat gatcaattat caaatctagg gaattacaat caagctgcta
     2761 gctggttcaa ccaaatcgga caagctgcat ttggtttcat gctgccggta ttagcaggat
     2821 ttattgcttc aagtatcggt gaccgccctg gtttgatcgt tggatttgct gctggtgcat
     2881 tagctaatac aggcggtgca ggattcttag gtgcactgat tggtggattc ttagctggtt
     2941 atgtcatcgt atttttacgt aaattattca agaacttacc aaaatcattg gatggaatta
     3001 aaacgatcct cttttatcca gtgtttggct tactgattac tggtttcttg atgttgctgg
     3061 tcaatgtccc tatgaaagca atcaatgatg cattgaacgg tttcttgaca agtatgagtg
     3121 gttcgaatgc tgctttactt ggtgcattat tagctggaat gatggcggca gatttaggcg
     3181 gtccaatcaa caaagccgct tatgtgtttg gtacagcaac tttagctacg actgttgcaa
     3241 ctggcggaag tgtcgtgatg gcttctgtta tggctggagg aatggtacct ccattggcaa
     3301 ttttcgttgc tacacgtttg tttaagaata aattcagcaa aacagatcaa gatgctggtt
     3361 tgacgaacat cgtgatggga ctatcgtttg tcacggaagg atcgattcca tttgcagcag
     3421 ctgacccgat tcgtgccatt ccaagtttta ttattggttc tgctttaact ggcggattag
     3481 tcggagcttt cgggatcaaa ttactggctc ctcatggcgg gattttcgtt gtattcttac
     3541 tgagtcaccc ggtcatgtat ttggtgttta ttgctatcgg agcagtcgta tctggtgtga
     3601 tttatggttt attgagaaaa gctcctgcag cagcagaagc atagaaataa aaaagaagag
     3661 gctggttttt cccagtctct tcttttttta tagaatcata tagaatcaag tagtgtagat
     3721 gaatagttgt tgtaaaatag tttttccatg ttcgttaaaa tgattgcttc aaatttgtga
     3781 tagaagattt agcctcgatt tcctgcttga ttcttggcaa gtttgcttca tcagtcaaga
     3841 taaccaaaat atctcctaca ttcatgatcg tatcaccgtg agtcaagatt tcctgatttc
     3901 ctctgcgtat agagattagc agcatatttt taggccaatt gaagtctcgt accattaatc
     3961 cgtctaaaga actctctgcc gttactggaa aatcaaaagt tgtccttgca ccttttagtg
     4021 aggcggtctg agtatttcca agtagacgtt ccaataatga ttcgtaaatt ggattaccgc
     4081 cgagaaggtc attgacgata taagcaacaa gagcagccaa tcctaaaggc atcagatgat
     4141 taagagagcc aaccatttct gtcactaaaa taattgctgt cagcggtgct tttccaatcg
     4201 ctgtgaaata accagccatg gcaataacga caaaatcctt gatgtagatt ggatccatcc
     4261 cgaaatcaac cataatgttt ccataaaagc taccgattaa agcccccaat gtcaatatcg
     4321 gaaggaaaat cccccctgga agaccaccac cgtaagaaat catggaaaaa acgaatcgaa
     4381 gaacaaacaa accgataatc ataatgattg gcaaggaagc atgtccaaaa gctaaaacga
     4441 tttgattgcc tccgcctaga gtactaggcc ataaataacc aattggcaaa atcagcaaga
     4501 aaggaacgaa cccgtgaaaa taagctggta aaggcaatcg gttgtaaaat tttggcaaag
     4561 ccagcaagac cttctgataa ataaaaccta agatgcctag aagtactcct aagatcacta
     4621 aggaaccata atatttcaac ggcaaagaag gaaggtcacc tagatataaa acaggtctca
     4681 agccgaaaaa gtaaagagag acgaagttag cagtgattgc tgaagcaaat gatgtcagcc
     4741 aaacaagtgg agaaaaggag tgatggattt cttctaaaac aaagagtaat cctgcgatag
     4801 gagcattgaa tgctgctgca agtccgctac ttgcaccgct agaaattaaa atcttttctt
     4861 ctgaagatgg actccgaaac aattgtccag ctccttgtcc aatggatgcg ccaagctgta
     4921 tagaaggtcc ttctctacct agaaaaaggc ccgcaccaac tgaaagtaca ccgccaatga
     4981 actttttcca taaaacagag aaccagttca ttgataattc cccgcgaagc tgtccctcta
     5041 cttgtggaat cccgcttccc ttgatatccg gttcattttt gaccagataa cccacgaaaa
     5101 atgctgcaag gatgcagcaa atagctaggc caattaagta aatcggctgt tcccgactca
     5161 tttcatagat acttcgaact aaatcgctta gcttttcgat agataatcga aacaggctaa
     5221 cgacgattcc tgtcagcagt ccaacaaata ttccttttag aataaaatat atctgagtct
     5281 tatctaattt cttgattttt cctttttttt ccattcaaac gcctcgcttt cttttaattt
     5341 agcgcgaaat caacgaattg acaaggaaaa atccataaaa agaaacaaat gattttgaac
     5401 tagctagttt aatgaaagcc aagaaaatgc tataattaac aagattgata aaagaaagga
     5461 tgttgttaat gtcatccaaa gctgaattat taaacgggat gaacccccgt cagaaagaag
     5521 ctgttcttca cactgacgga ccattactgc taatggcagg tgcaggaagt ggaaagacga
     5581 gagtgctgac acaccgcatt gcctatttga ttgaggaaaa agaagtcaat ccttggaaca
     5641 tcctagcgat tacttttacc aataaggctg caaaagaaat gaaagaacgt gtcaatgcga
     5701 ttctcgcatc tggaggcgaa gatgtctggg tatcgacttt tcactcgatg tgtgtgcgta
     5761 ttttaagaag agatgttgat tttatcgggt ataaccggaa ctttacaatc atcgattctt
     5821 ctgaacagct gaccttgatg aaacggatac taaaagaact gaacattgat ccgaaaaaat
     5881 atgatcctcg aagtatatta ggaacaatct ctcaagcaaa aaacagcctg cagacccctc
     5941 aggattttgc aaaaatgcaa ggcagttatt acgaagagat tgcggcaaaa tgttatgctg
     6001 cttaccaaaa agagcttcaa tataatcaat gtatggactt tgacgacctg atcatgaata
     6061 cgattcgatt atttgaagaa catcctgatt cactcacata ttatcaaaac aaattccatt
     6121 atattcatgt agatgagtac caagatacaa atcacgcaca gtatacgcta gtcaatttat
     6181 tggcaggtag atttaggaac ctgtgcgtcg tgggagatgc agatcaaagt atttacggct
     6241 ggcgcggtgc agatatgcag aacatcttgg attttgaaaa agattatcca gatgctgcag
     6301 taattttgct agaacaaaac tatcgctcca cgaaaaacat ccttagcgca gcaaaccaag
     6361 tcattgaaaa caatagcaat cgaaaaccaa aaaacttgtg gacagaaaac aaagaaggaa
     6421 acaagatcac ttactaccgt gcagataatg aacgagacga aacccgtttt atcgtagatc
     6481 ggatgcaaga agagattcga agcaatcatc ggaattacgg cgactttgcc atcttgtacc
     6541 ggacgaacgc acaatctcgt gtaatggaag aaactctgtt gaaagcaaat attccttata
     6601 aaatggttgg agggcacaag ttctacgatc gtaaagaaat caaagatatt ttagcgtatt
     6661 tgaatgtgct ggcaaatcct caagattcaa tcagttttga gcgtatcgtt aattcgccca
     6721 aacgaggcat cggaccagga tctatcgaaa aactacgcag ttttgcttct cttcatgaat
     6781 ggccactgct tgaagcagcg caaaatgtgg atctggcaaa tatcggtgga aaagcaggac
     6841 agcaattagg tgcatttgga gagatgatcc aagaagtcac tcagatgatc ccatacttga
     6901 cagtgacaga attgacgaaa gaggttttgg atcgtagcgg atatctagaa gacttaaaaa
     6961 tccaaaatac attagaagca caagcacgca tcgaaaactt ggaggaattt ttaacggtca
     7021 cacaagaatt cgacaaacaa ttcgaacagc aaaatgaaga agacgctgat gcaccagaag
     7081 aaaaactaac ggtcttctta aatgatctgg cacttgtttc cgatattgac aatttagaag
     7141 aagatgcttc ccaagtgaca ttgatgactt tgcatgcagc caaaggatta gaattcccag
     7201 ttgtattcct gattggtcta gaagaggggg tttttccttt atctcgagca ttgatggaag
     7261 aaagcgaact ggaagaagaa cgccgtctcg catatgtggg gatcacgcga gcagaagaag
     7321 cgttgtattt aacaaatgct ttctccagaa cattatatgg acggacgcaa tataaccgac
     7381 cttctcgttt tgtagaagaa atcgatcagg agttactgga aatcgaaggc atgcgtccta
     7441 cgcctaaaaa aacacctgta ttcgctaaaa aaacagcata tagctataaa cagccagaga
     7501 cagcggttgt tccctctaaa agtgctactg gaggggaaaa taacagctgg aaacctggag
     7561 ataaagtcaa acacaaaaaa tggggtctag gaacagtcgt ccgagtaagc gggacgtcga
     7621 aggatctaga actagatgtg gctttcccaa gtcaaggagt aaaacgtctt ttggctgcct
     7681 ttgcgccaat tgaaaaagct tagtagtgga gggtaaatga tggaacatga aatgacgatt
     7741 gacgaagctg ctaaacgagc agaagaactt cgtacaagac tgaaccaatg gtctcgcgaa
     7801 tactacgtag aagataaacc aacagtggaa gactatgttt atgataaaga atacgcagaa
     7861 ttagtagcaa ttgaggaaca atatcctgat ttgatcacgt ctgattctcc cactcaacga
     7921 gtaggcggaa aagtattaga aggttttgaa aaagtcactc atgatattcc gctttacagt
     7981 ttgaacgacg ttttcagcaa agaagagctg atagcttttg atcaacgtgt acaaaaagca
     8041 gtgggacgtg tagttgacta ttgctgtgaa ctaaaaatag atggactttc cgtttctttg
     8101 cgttatgaag atggaaattt cgtgagaggt gcgactcgcg gggatggaac tgtaggagaa
     8161 aacatcactg aaaacctaaa aactgtccgg tcggtcccaa tcaaattaaa agaaccaatg
     8221 aacatcgaag taagaggcga atgtttcatg ccaaaacggt catttgtcca attgaaccaa
     8281 gacagagaag cagagggaaa agatatcttt gcgaatcctc ggaacgccgc agctggttct
     8341 ttgcgtcagt tagattctaa aatcacagcg aaaagaaatc tggacacttt tttatacaca
     8401 gtagctgatt tcggtcctat gcaagcaaaa acccagtatg atgcgctaga agagctagaa
     8461 aaaattggat tccacacgaa tcgggaaaaa cgtctatgtc attcgattga tgaagtgtgg
     8521 tcatacattg aagaatatca tgacaaacgt gtggatttgc cctatgaaat cgatggaatc
     8581 gtcatcaaag tgaatgaatt ctcgctacaa gatcaacttg gttttactgt caaagcaccg
     8641 agatgggcag cagcttacaa gtttccgcca gaagaagttg aaacgctaat cgaaaacatc
     8701 gaatggacag tcggtcgaac aggggtagta acgccaacag ctatcatgac accagtccga
     8761 gtagcaggaa cgacagtaag ccgtgccagc ctgcataacg gtgattatat taagttgaag
     8821 gatatccgct tgaaagatac agttctgatt tataaagcgg gagacatcat cccagaagtt
     8881 tcgcaagtcg tgttagataa acgacctaaa gacagcgaag aataccaatt gcctacacat
     8941 tgtccagttt gtggaagtga acttgtccat ttggatgaag aagtagcctt acggtgcatc
     9001 aatcctaaat gccctgctca aatgaaagaa ggtttgaacc actttgtttc aagaaatgcg
     9061 atgaatatcg atgggttggg gccgcgcgta ctagaacaga tgtatgataa aaaactagta
     9121 gcagatgttg ctgatcttta caaattgaca gaagaagagt tattgacgtt ggacaaaata
     9181 aaagaaaaat cagcaaataa tattttgaca gcaatcgaca acagcaaaga taattcggtc
     9241 gagcggttga ttttcgggtt aggaatccga catgtcggag cgaaagcagc aaaaatttta
     9301 gcagaacatt tcggtgatct agaaacattg agtaaaagtg actatgagtc gattattgcg
     9361 cttgatacga tcggtgatat cattgccgac agtgtagtca cttatttcag taacgaagaa
     9421 gtccatgaat tgatgaatga attaaaacaa gcaggcgtca atttcgaata caaagggctt
     9481 cgaaatgccc aattgcaaga agtcgagtct ccttttaaag aaaaaacagt agtccttact
     9541 ggtaaactaa cacgatttac tcgtgaagaa gcaaaagaga cgatcgaaaa tcttggcggg
     9601 aaagtgactg gtagcgtatc gaaaaaaacg gatatcgtgg tagcaggaga agatgctggc
     9661 agcaagctga ccaaagcaca agaattagga atcgaagttt ggacagaaga tcaaatggca
     9721 gatgcattgg ctaaaagtcg ctcagtagaa gaatgaaaaa tgagtttttt cagacaaatt
     9781 tcaataaaca atttcagaca cggcactctt gtgttaaaat agagagcaaa gtttttaact
     9841 cgtttagaaa gaaggggtag tatggcgatt agtgaagaac aagtgaaaca cgttgccaaa
     9901 ttagcaaaac ttgcgttcgc agatgaagaa cttgcaagtt ttaccgacca attaggtaaa
     9961 atcatcgata tggttgaaat gctggaagaa gtagatacag aaggtgttcc atttacgtcg
    10021 aatgtggttg aaacagtaaa cgtgatgcga caagatcggg cgcaagctgg atggaaccgc
    10081 gatgaattat tgaaaaatgt gccggaaaca gaagacggct ttatcaaagt acccgcaatc
    10141 atcgataatg gggaggctgg cgcataatga cagaattaca caatcgttct ctagaagaac
    10201 tgcatcatat gcttgtatcg aaagaaatca ctgtacaaga tttgacaaaa gcaacatttg
    10261 aacgaatcaa agaaacagag cctgaggtcg aagcattcat tacattgaac gaagaaaaag
    10321 cattggaaca ggcaaaagct ttagatgaaa aagggattga tgaaagcaac gtccttgctg
    10381 gtattccaat tggtatcaag gataatatcg taacaaaaga tatcttgaca accgctgcat
    10441 ctaagatcct ttacaatttt gaaccaattt atgatgcaac ggtcatggat aaaatctatc
    10501 aagcagatat gatccctgtt ggtaagctaa acatggatga atttgctatg ggtggaagta
    10561 cagaaacttc ttatttcaag aaaacaaaaa atgcttggga cccaacaaaa gtacctggtg
    10621 gctcttctgg tggttctgca gcagcagttg cagctggaca aattccagtt tctttaggta
    10681 gtgatactgg gggaagtatc cgccaaccag cagcttttaa cggcatcgta gggatgaaac
    10741 ctacttatgg ccgtgtctca cgttttggtt tgattgcctt tgcttcatct cttgaccaaa
    10801 tcggaccaat gacccgtacg gtgaaagata atgcattagc attgacagct atcagcggtt
    10861 atgacgaaaa agacggtact tcttcaggcg tttcagttcc tgattttgca gagggactga
    10921 caggcgacat caaaggaatg aagattgctt tacctaaaga atacttaggc gaaggtgtgg
    10981 cccctggcgt aaaagaagct gtattgaaag cagcagaaac attccgttca ttaggtgctg
    11041 ttgtggaaga agtcagcttg cctcattcaa aatacggtgt ggctgtttat tatatcattg
    11101 cctcatcaga agcaagttcc aacttgcaac gattcgatgg tatccgctac ggttatcgct
    11161 cagaaaatgt gaaatcatta gatgatgtat atgtcaactc acgttcagaa ggattcggtg
    11221 aagaagtaaa acgccgtatc atgttaggta cattctcctt aagtgctggt tattatgacg
    11281 cttacttcaa aaaagcggga caagtccgta cattgatcaa acgagatttt gaaaaagtct
    11341 ttgcagatta tgacttgatc atcgggccat cttcaccaac agttgcattc ggaatcggtg
    11401 agaacattaa cgatccaatc acaatgtaca tgagtgacat cttaacgatc cctgtcaact
    11461 tggctggact tccaggtatg tctgtaccgg caggtttatc agaaggatta ccagttggat
    11521 tgcagatcat cggtaattat tttgatgaaa aaacgatgta tcaagcggca tatgcgtttg
    11581 aacaagcaac agaattccat aaacaacaac cagctatttt aggagggaaa gacgaatgaa
    11641 ttttgaaaca gtcattggac tagaagtcca cgtcgagtta aaaaccaact caaaaatttt
    11701 ctctccagcg ccagctcatt ttggtgcaga accaaacagc aatacaaacg tgatcgactg
    11761 gggttatcca ggcgtgctgc cagtaatgaa taaagctgcg ctggaattcg ggatgaaagc
    11821 agctttggca ttaaactgtg aaatctctaa agatactcat tttgaccgga aaaattactt
    11881 ttacccagat aatccaaaag catatcaaat ttcccaattt gatcaaccaa tcggtcatga
    11941 cggatggatc gaaatcgaag tagaaggtaa aaagaaaaaa atccggatcg aacgtgtcca
    12001 tttagaagaa gatgctggaa aaaacatgca tggtattggc ggttattctt acgttgattt
    12061 gaaccgacaa ggcacgcctt tgatcgaaat cgtttctgaa gctgatatgc gttcaccaga
    12121 agaagcatac gcttatctag aagcattgcg atcaattatc caatttacag aagtcagcga
    12181 tgtgaaaatg gaagaaggct ctatgcgctg tgatgccaat atttctcttc gcccttatgg
    12241 acaagaagag ttcggtacga aagcagaatt gaaaaacttg aactctttat ctttcgtgaa
    12301 aaaaggttta gcttttgaag aaaaacgtca agcaaaagtt cttctttcag gaggagaaat
    12361 ccaacaagaa acacgccgtt ttgacgaaac aacgaataaa acagttttga tgcgtgtcaa
    12421 agaagggtct agtgattatc gttacttccc agagcctgat atcccgaaat tcgtgatcga
    12481 tgacgaatgg attcaaaaag taaaagctag tttgcctgag atgccagctt ctcgtcgcga
    12541 acgctatatc cgtgagtttg gacttccaga gtatgacgca atggtattga cacttacaaa
    12601 agaaatgtct gatttttttg aagctgtttt ggaaaatggt gcagatgcca agcaagcatc
    12661 taactggttg atgggtgaag tttctgctta cttgaacagt gagaaactgg aattagctga
    12721 gacaaaattg acacctgaga acttagcagg aatgattcgt ttgatcgccg atggaacgat
    12781 cagctcaaaa atcgccaaaa aagtcttccg tgaattgatc acaaatggcg gagaagccca
    12841 agaagtagtt gaaaaaaatg gttgggtgca attatctgat ccgaacaaac tactgccaat
    12901 cattaatgaa attttggata acaaccaaca atctgtcgat gatttcaaaa atggaaaaga
    12961 tcgtgcggtt ggcttcctag tcggtcaaat catgaaagca actaaaggac aagcaaatcc
    13021 tggtgtcgtg aataagctac ttaaagatga attagcgaaa cgatagaggc tgaaaatgaa
    13081 aaaagcacgc gtaatttata accccacctc gggaaaagaa ttattgaaaa aaaatttggc
    13141 ggatatccta caggcattag aagaagcagg atttgaagcc agcgcctatg caactacacc
    13201 agaaccagat tcagccaaga atgaagcatg tagagtcgca gaactaggtt ttgatctagt
    13261 agtggcagct ggtggcgatg gcacaatcaa cgaagtggtt aatggaatcg ctccgcttga
    13321 acaccgtcct aaaatggcca tcatcccagc tggtacaacg aacgactatg ctcgagcatt
    13381 aaaaattcct agagataata ttaaagcagc agcagaagtg ataaaaaaac accaaaccgt
    13441 aaaaatggac atcggccttt ctgataacag ttacttcatc aatatcgctg caggtggcta
    13501 tttgacagaa ttgacttatg aagttccatc agaactaaag agtatttttg gttatcttgc
    13561 ttatcttgcg aaaggagcag agatgctccc tcgagtaaag ccaatcaaaa tgcgcttgaa
    13621 gtatgatgaa ggggaatata tcggaaatgc ttcgatgttt ttcctagggc tgacgaactc
    13681 tgttggagga ttcgaacaga tcgcgccaga tgccaaattg gatgatggga agttttcttt
    13741 aatcattgtg aagacagcaa atatctttga gatcttgcat attgcagcac tcatgctgaa
    13801 tggcggtcga catgtcgatg atccaagaat aatctatacc aaaacaagca gtttgttcgt
    13861 tgaaacagaa gatgatatca atcgaccagt aatgatcaac ttagatggtg aatacggcgg
    13921 ggaagcaccg atgcatttcc aaaatcttca tcaacacatt gagttctttg ccaataccga
    13981 tcagattcct gatgaagcaa tcacaggtac agatgaagaa gaattagagg ttgtcagcaa
    14041 agaattcgta aaagaagtcg aacgtttaac ggatgaagat atcgacggtg acggaaaaat
    14101 agcagacgaa acacctaatc aaaagaatta atcaaaagcc ggtgctgaac gtagtaaggt
    14161 cagtgccggt tttagttgtg taattagctg atttatagta gagttcagta ggattgaaaa
    14221 ataggcttaa aggagcaaaa tatgacagaa cagcaaatag taaagaaaaa cgaacgatat
    14281 acagtcgaaa tcgtcgactt gagttatgaa ggaatggggg tagcaaaaat cgatggttat
    14341 ccattattta tcgaaaatgc actccctgga gaagaagtag aaatactagt agtaaaagca
    14401 ggcaataagt ttggatatgg aaaagtcgaa acatttatta cacaatcacc tgatcgtcag
    14461 cctgtagaaa acaatgcatt acttcgcata ggtatcgcac ctttagccca tttgaagtat
    14521 gaacaacagc ttgtcttcaa acaaaaacaa gtagagcatg taatggcgaa gattgctaag
    14581 atgccagaag ttcctgtact tccaacggtc ggaatggcag aaccattcgg ctaccgtaat
    14641 aaagcacaga tccctgttcg ccgtatcgat ggacagttag ccaccggttt ttacaaaaag
    14701 aatagccatg atctagtccc tatcgaagat ttttatatcc aagatcctgt catcgatcaa
    14761 gcaatcaaag tagtcagaga tatcttgcaa cggttccaag tacgaggata taatgaagaa
    14821 aaaaatgaag gacagcttcg tcacatcatt atccgtcgtg gtcattattc ccatgaaatg
    14881 atggtcgttt tagttactcg taaagaaaaa ttctttaaat ccaaggaaat tgctgaagct
    14941 attcatgaag aattgccaga agtggtttcg gtcatccaaa atatcaatga agaaaaaaca
    15001 aatgtgattt taggagacaa agaaaaagtt ttatatggca gagactatat cgaagatcag
    15061 ctattaggaa aaacctatcg aatctcagca aagtcatttt atcaagtaaa tactcaacaa
    15121 gcagagtatc tgtaccaaac agccatcgag tttgccgagt tagataaaaa agacattgtg
    15181 atcgacgctt attcaggaat aggtacgatt ggcttgtctt tagcagacag agtggataag
    15241 gtgtatggga tggaagttat tccagaagca attgaagatg cacagttcaa tgctttaaat
    15301 aataaaatcg aaaatgttca ttatgaagtc ggaaaagcag aaaaagttat gaaaaaatgg
    15361 caagaaaaag ggatcaaacc atctgtgatc gtagttgacc ctccacgaaa aggattagat
    15421 catcatttta ttgaatcagc aatcgaaatg tcaccagaac gaatcgtcta tatctcatgt
    15481 aatccggcaa cttttgcaag agatgctaag ttatttgcag aagcaggtta tgccgtgaag
    15541 aaagttcagc ctgtagatct tttcccgcaa acccatcata tcgaattagt cgcttcattc
    15601 gttaaaggag aataaaaatg gaccaaaaag aaaaaatgaa agaattgatc aaaaagaaac
    15661 aaggtggagg ccgatccaaa caaatggaaa cacctaagaa tgatcgtaaa aacatgagaa
    15721 aaggccctaa aatctttaat aaatagacgt gctaaagaaa gactaagaag tgttaacttt
    15781 tgttattgtt tcttagtctt ttta
//
LOCUS       DAXWLF010000052        15686 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXWLF010000052 DAXWLF010000000
VERSION     DAXWLF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.052"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(73..792)
                     /locus_tag="ACGE4U_001270"
     CDS             complement(73..792)
                     /locus_tag="ACGE4U_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8393446.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(923..2074)
                     /locus_tag="ACGE4U_001271"
     CDS             complement(923..2074)
                     /locus_tag="ACGE4U_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFF8393447.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2291..3031)
                     /locus_tag="ACGE4U_001272"
     CDS             complement(2291..3031)
                     /locus_tag="ACGE4U_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8393448.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3035..3391)
                     /gene="rsfS"
                     /locus_tag="ACGE4U_001273"
     CDS             complement(3035..3391)
                     /gene="rsfS"
                     /locus_tag="ACGE4U_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFF8393449.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(3415..4008)
                     /gene="yqeK"
                     /locus_tag="ACGE4U_001274"
     CDS             complement(3415..4008)
                     /gene="yqeK"
                     /locus_tag="ACGE4U_001274"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFF8393450.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDVEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3992..4642)
                     /locus_tag="ACGE4U_001275"
     CDS             complement(3992..4642)
                     /locus_tag="ACGE4U_001275"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFF8393451.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4652..4963)
                     /gene="yhbY"
                     /locus_tag="ACGE4U_001276"
     CDS             complement(4652..4963)
                     /gene="yhbY"
                     /locus_tag="ACGE4U_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFF8393452.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4979..6088)
                     /gene="yqeH"
                     /locus_tag="ACGE4U_001277"
     CDS             complement(4979..6088)
                     /gene="yqeH"
                     /locus_tag="ACGE4U_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFF8393453.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6085..6612)
                     /locus_tag="ACGE4U_001278"
     CDS             complement(6085..6612)
                     /locus_tag="ACGE4U_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFF8393454.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6839..7624)
                     /locus_tag="ACGE4U_001279"
     CDS             complement(6839..7624)
                     /locus_tag="ACGE4U_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFF8393455.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(7617..8486)
                     /gene="accD"
                     /locus_tag="ACGE4U_001280"
     CDS             complement(7617..8486)
                     /gene="accD"
                     /locus_tag="ACGE4U_001280"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFF8393456.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8487..9866)
                     /locus_tag="ACGE4U_001281"
     CDS             complement(8487..9866)
                     /locus_tag="ACGE4U_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFF8393457.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9874..10293)
                     /gene="fabZ"
                     /locus_tag="ACGE4U_001282"
     CDS             complement(9874..10293)
                     /gene="fabZ"
                     /locus_tag="ACGE4U_001282"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFF8393458.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10318..10794)
                     /gene="accB"
                     /locus_tag="ACGE4U_001283"
     CDS             complement(10318..10794)
                     /gene="accB"
                     /locus_tag="ACGE4U_001283"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFF8393459.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10797..12032)
                     /gene="fabF"
                     /locus_tag="ACGE4U_001284"
     CDS             complement(10797..12032)
                     /gene="fabF"
                     /locus_tag="ACGE4U_001284"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFF8393460.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12051..12788)
                     /gene="fabG"
                     /locus_tag="ACGE4U_001285"
     CDS             complement(12051..12788)
                     /gene="fabG"
                     /locus_tag="ACGE4U_001285"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFF8393461.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12800..13717)
                     /gene="fabD"
                     /locus_tag="ACGE4U_001286"
     CDS             complement(12800..13717)
                     /gene="fabD"
                     /locus_tag="ACGE4U_001286"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFF8393462.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13824..14048)
                     /locus_tag="ACGE4U_001287"
     CDS             complement(13824..14048)
                     /locus_tag="ACGE4U_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFF8393463.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14101..15063)
                     /locus_tag="ACGE4U_001288"
     CDS             complement(14101..15063)
                     /locus_tag="ACGE4U_001288"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFF8393464.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15073..15513)
                     /locus_tag="ACGE4U_001289"
     CDS             complement(15073..15513)
                     /locus_tag="ACGE4U_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8393465.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4116 a         3571 c         2687 g         5312 t
ORIGIN      
        1 gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt
       61 aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag
      121 atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac
      181 ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt
      241 taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc
      301 ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc
      361 agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc
      421 ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg
      481 attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc
      541 atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc
      601 aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg
      661 gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt
      721 tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca
      781 tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc
      841 ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt
      901 cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg
      961 gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg
     1021 cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc
     1081 ttccaacatc ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct
     1141 cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat
     1201 atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg
     1261 ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag
     1321 tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaat
     1381 aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt
     1441 ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat
     1501 gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat
     1561 tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat
     1621 cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag
     1681 acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac
     1741 tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg
     1801 tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga
     1861 cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt
     1921 tatcgatgat agccggttcg cctcgttgca aaaaattccc gctcattaca gcaatgacga
     1981 cctctgctcc tgttgttttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag
     2041 gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat
     2101 aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat
     2161 aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc
     2221 tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca
     2281 ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg
     2341 cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc
     2401 ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc
     2461 gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct
     2521 cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat
     2581 attctggaaa aatttcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat
     2641 tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca
     2701 tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat
     2761 cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg
     2821 caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa
     2881 aggcaactgc taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt
     2941 gtaaatgacg tttcgaaaaa gctagccatt gctgataaag tgtttcgtcc atcacttcat
     3001 cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga
     3061 ctagcggagc atctgaccat agtttttcca gattatagaa acttcgttct ggtgcatgga
     3121 aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt
     3181 caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg
     3241 cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa
     3301 catttcgaat gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaaccg
     3361 caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt
     3421 ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc
     3481 aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca
     3541 tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc
     3601 tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat
     3661 tgcatgaaga atttctacat cggtgattcc taattcccgc tctacaaacg aagcgccaac
     3721 aagaccatgc cagattgcat tattataatt caataactct ggatcaaaac cgtctcttac
     3781 gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc
     3841 aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc
     3901 tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag
     3961 gttttctcta gaataagcag tatattcatt actatagtcc atattcatac aacccttcat
     4021 tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga
     4081 tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag
     4141 gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag
     4201 tcagctcatc gattttatac cattttggca aatattctac catatcccca ccgataataa
     4261 agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac
     4321 ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccacag
     4381 ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag
     4441 gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt
     4501 cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt
     4561 gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg
     4621 tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc
     4681 tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga
     4741 cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg
     4801 tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa
     4861 cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg
     4921 ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt
     4981 aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta
     5041 ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca
     5101 cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct
     5161 gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat
     5221 gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat
     5281 caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag
     5341 gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca
     5401 tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa
     5461 tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc
     5521 ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga
     5581 cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct
     5641 catgagattt cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag
     5701 cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca
     5761 ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta
     5821 gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct ttctgcccta
     5881 attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg atctcattat
     5941 aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca
     6001 aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac
     6061 agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg
     6121 ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca
     6181 actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc
     6241 acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa
     6301 ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa
     6361 aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac
     6421 aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc
     6481 tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag
     6541 attctttaat tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta
     6601 atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc
     6661 aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca
     6721 gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa
     6781 acaaaaaaca actaagcgag caagaccatt ctttactctt tgcttagttg ttaaacacct
     6841 aatattggcg gaacctcttg tatctttttt ccagtaattc atgctggtcg aatcgaacaa
     6901 gttccgtcaa tctttcaatc aacgcttttt tcataatgtg tgcgacttta tatcgtggca
     6961 actgctcgcc gttgaacgtt tcaggaataa ctcgatcaac gattcctaac tcgcgtagtt
     7021 ctggcgcagt aattttcatc aactcagctg cttctgcggc gcgactaccg tctttccaca
     7081 aaatcgacgc aaatccttct ggagaaagca cagcataaat cgtttcttcc agcatccaga
     7141 cttcatccgc taaacctaga gcaagggcac cgccacttcc accttcacca atgatgatgg
     7201 cgatgatcgg tacctttaaa tcagacattt cgagcagatt tctggcaata gcctctcctt
     7261 cgccgcgctc ttctgctcct acaccgcaat atgcacctgc tgtattgatc aatgtaatca
     7321 ccggtctatt gaacttttcc gcttgtttca tcagtcgtaa agcttttcga tagccttcag
     7381 gatggggaga accaaaattt ctgcggatat tttcctgcag atcccgtcct ttttgaatac
     7441 cgatcactgt taccggtttg ccatctaatc gagcgattcc accaacaatc gcttcgtcat
     7501 ctgcaaacaa acgatcacca tggcattcct cgaaagaatc gaagatctct tcaaaaaaat
     7561 ccaagcttgt ccatcgttct ttagctcttg ctaactgaac gacatcatgt gctgtcttat
     7621 ccacgatcta tccatccttt ctgtgtatgc aattcaagaa gcgtatggat tttctgcttc
     7681 aattccatgc ggggaacaat ctgatcgaca aaaccatgag ataataagaa ttcagccttt
     7741 tggaaatctt ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct tccagcaaaa
     7801 ccgatcaagc tttggggttc tgctagaatg atatcgcctt ccattgcaaa actggcagtt
     7861 actcctccgg tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc attgctatgc
     7921 cgtttgacag cagcagagat ttttgccatc tgcattagag aaaagattcc ttcttgcatc
     7981 cgtgcgccac ctgaagctgt gaacaagacg actggaagac tttcctttgt tgctcgttcg
     8041 aataatctgg tgatcttttc tccgacaatc gttcccatac ttcccatgat aaaattagga
     8101 tccatcactc caataatgaa gggaatccct tgaatggttg ctttaccagt gaggactgct
     8161 tcatgaagtc ctgttttatc ttgcattttt ttaatttttt ccgtatattc gggaaatgct
     8221 agcggatctt tggttacaag atccgtatcc cattcttcaa aacttttttc atcaataatc
     8281 aaagctagac gctgccatgc accgattcga aaagcgtatc cgcaatgagg gcatactttt
     8341 tcttcaccga tgtctttcgt ataaaggata tgtttgcaat ttggacactt tgcccacatg
     8401 ttatcaggaa cagatggcga gttagcaggt ttgctctctc tattaggatt gatccgaata
     8461 tatttctttt tcttaaacag agccatctat gcgcctccta tctccgtttc tggtgtccaa
     8521 ttcggtaaaa attcttcttg taaaaatgct gtgctgtaat cacctgctat gactgcaggg
     8581 tgactaatca aatccatctg aaattctgcg ttcgtgatga ttccttctgt gataaattca
     8641 cttaaggctc gctgcatctt cattaatgct tcaaaacgcg tatttccatg gacgattatt
     8701 tttgcaatca ttgaatcata atatggcggg atcgtatatc ccgggtaaac tgcactgtct
     8761 acccgcagtc ccatcccacc tgctggaagc agtaagtttt ggatcttccc tggagaagga
     8821 gcaaaatgga aagcaggatt ttctgcatta atccggcatt cgattgcgtg tccttggaat
     8881 actacttctt cttgacacac attcaaaggc tcaccggctg ctatctccac ctgttttttt
     8941 actaaatcga ttcctgtgac catttctgtt actggatgtt ccacttggat tcgagtattc
     9001 atttccatga aataaaaatc gccttcttca tccatcagaa actcgatcgt tccggcattt
     9061 tcataattga ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc tctttttgtt
     9121 tgtgagatca ctacagaagg agattcttcc agtacttttt gattattacg ttgcaaagaa
     9181 caatctcgtt ctcctaaatg aatgacattg ccaaaatgat cacctaaaat ctgaacttct
     9241 atatggcgag caggataaat gattttttcc aaatacatat catcatttcc aaatgccgct
     9301 gctgcttctt gctgagctga agaaaaatgc tgtgggagct cttcttttga ttgaactttc
     9361 cgaatgcctt tcccgccacc acctgcagct gctttcagca tcactggata accaattctg
     9421 tctgctattt ccaatgcttc ttctactgta gataatacac cagtacttcc tggaatgact
     9481 gggacatttg cttcttgcat caattgacgc gcattgatct tatttcccat tgcatcgatc
     9541 gttgcacttt taggaccaat aaatgtgata ttgcactctt cacacatgga ggcaaatcgg
     9601 ctattttcag ataaaaaacc aaatcctgga tgaattgctt cggcttttgt cacgattgca
     9661 gcgctcagta cttcctgaac attcagatat gaatcaacgg cttttgccgg gccaatgcag
     9721 attgcttcat ctgctaactg tgtatgaagt gcttcggcat ctgcttctga atatatcgct
     9781 acggtttgga tccctagttc gcggcatgca cgaatgatac gtacagcgat ttcaccgcgg
     9841 ttagctatca atactttaga aaacatattt cacctatcca atcataaagg ttaattccgc
     9901 ttcggctact tttttaccgt ctactttagc gattcctttg cctattccgg ctgaagcttt
     9961 cactttcaat atctcaactt caaggtataa ggtatctccc ggtgtaactt tcttacggaa
    10021 ttttgctttg tccaatccgc cgaaataagc cgtctttccc ttgaattgtt caagagaaag
    10081 taatgcaact gctcctgctt gagccatcgc ttcaacaatt aatacccctg gcataacagg
    10141 ttcttctgga aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt tcttagcaac
    10201 gatccgttct ccttcaacca tttcttccac tcgatcaatc aaaagcattg gataacgatg
    10261 tggaatgatt tcttttattt cttgtatgtt cattgatttc cctctttcct gctttgctta
    10321 tttttgctga atcctaaaga gtggctgtcc atattcgaca actgactcgt tttctacaag
    10381 gatttctgtg atcactccgt cggcctcact agttatctca ttcatcagtt tcatcgcttc
    10441 aataatgcag ataacttctc ctttggcaac ttgatcacct acttttttga aagctggttt
    10501 gtcgggcgcc ggttgcagat aaacgattcc cacgattggc gaaacaacct cgattccatc
    10561 aaatactgcc gattcttttt ctttcattgt ttctggagca gttgttgctt gttcaggtat
    10621 gacattgact ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat tcatgcctcg
    10681 acttgtttta tttttattca tatacaattc aaattgacct tcacgcaaat cgaattccgt
    10741 tagcgaggat tgatcaaatt gactgactaa ttctctgatt tcattgatat ccaaaattat
    10801 tcctcccagc gtttcatgca cagaaccgca ttatgtccgc caaaccctaa tgaattactc
    10861 attgcataag taaaacttgc aggtttgctt tcgtttttga caactgtcaa gtcaatctca
    10921 ggatcttgtt tttctacatt gatcgtaggt gggataaatt gatgttctaa tgccagcaaa
    10981 gttgccaagg cttcgatccc gccggctgct cccaataaat gtccagtcat cgatttcgtg
    11041 ctgctgacgt aagtatctct gaaattttca cctaatgcga tttggattgc ttttgcttct
    11101 gcctggtcat tggctggtgt acttgttccg tgagcattga tatatccgac ttgttccggc
    11161 tgtacaccag cttcttccat cgcaagcttc attgctttag acgcaccaga accatctgga
    11221 gtaggagctg tcatatggta agcgtcacag tttgcaccgt atccaatgat ttctccatag
    11281 attttggcac cgcgagcttg tgcatgttct aaagattcaa gaactagaac acctgctcct
    11341 tcacccatca caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg attaggatca
    11401 gttgattctg agagtgctgt taaagctgca aatccagcaa tccccatctc acaaatcgtt
    11461 gcttctgttc ctccagcaag aatgacatca gaatagccat gtttgatgtt acggaatgct
    11521 tccccaattg aattcgttcc gctagcacaa gctgttacga tagctgtaca aatacctttt
    11581 gcacctacgc gcaaagcaat atttccagct gccatgttag caatcgccat tggtacgaac
    11641 attggagaaa ctcgttttgc acctttttca tgtagacgaa taacttggtt ttcgattgtt
    11701 ggaagaccac cgatcccaga tccgatgatg acgccaaacc ggtcttgatt cattttttct
    11761 ttatctaaac cgctcatctc cattgcttct aaagcagcgt agattccata gatcgaaaac
    11821 atatccattc gtttgctatc ttttttaata aaatatttat cgtaagggaa atctttgact
    11881 tcacccgcta aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg tgcgatgcca
    11941 ttcgtcccct cttttaagct attcaaaaag ctgtctgcgt catttccaat aggtgaagtg
    12001 actccatagc cagtaattac cactcgattc atttgtttgc tctccttttc ttatccgtgc
    12061 atgaccaatc cgccatcgac attgatgact tgtccggtga tatatggatt ttgtgctaag
    12121 aaaactgctg tttgtgcaat atcttctact tgtccgaaac gctgcatcgg aatttgcttt
    12181 tccgcttgtt ccttgacttt gtctgctaaa acttccgtca tatcagttgt gatgaatcct
    12241 ggcgcaatgg cattacatgt aatgcctcta gttgcagctt ctcgtgccac agattttgtt
    12301 aatcctacta cgccagcttt actagctgca taatttgcct gaccaatatt tccaatcaac
    12361 ccagatacac tagagagatt aatgatggcc ccttctcgct gcttcatcat ttttttcaat
    12421 acgtgctgcg tcatattgaa ggttccaatc aaattgatat caagacattt tttgaaatct
    12481 tctgcatcca ttcgcatcac aagtttgtca tttgtgattc cggcattgtt tactaacaca
    12541 tggatcgatc caagcttttc ttctgcttct ttgatcattt gtcccgcttt ctcataatca
    12601 gagatgtcac cggaaactcc tacgcatttt acaccgaatg cttcgatttc ttctatcttt
    12661 tcttttggaa tctctccgcg accattcaaa atgatatttg ctccagcttt tgcaaaagct
    12721 aaggccatcg ctttgccgat tcctcttgta ctacctgtga taaatacatt ttttcctttt
    12781 acatccataa cgttcctcct tatagtattg ctattgtttc atcaaatgtc tgcttattct
    12841 caacacgcag aactggtatt gttttatcga tttttttcat aaatccgctt aatacttttc
    12901 cggggccgac ttcgatgact tgttcgatgc cgattgtttt taacttatgg atactctcat
    12961 agaaacgaac aggttgcatg acttgctgtt caagtagtcc cgcgattgtt tctttcttca
    13021 ttatttccgt tgttgtgttg cttatgatag gaaaagaagg ttcagagaat tgaatctgac
    13081 ttaggtcctt cgccagtttt tttgctgccg gttctaaaat agctgtatga aaaggcccgc
    13141 tgacatttag agggatcatt cttttgaacc ctttttcttt taatagtaaa acagcttcgt
    13201 ctactgcttt ttcttcgcca ccaatcacga tctgctgagg tgtattataa tttgcaggag
    13261 atacgatgcc gtacttgcta gcttcatgac agctttcctc gatcgtttca atcggcgcat
    13321 tcatcactgc aaccattttc ccgcttccag ctggggcagc ttctgtcata taagcgcctc
    13381 tttttgctac taacgcaact gcttcagtga aggatagcgc attactagct actaatgccg
    13441 aatattctcc taaactcagt cctaaagctg catctgggat gattccatgc tccttcaata
    13501 agcgataata cgcgatgctg actgttagaa tagctggctg tgtatattgc gtctgatcca
    13561 aacgttcatt ttctgtaaag caaagttcag ccatatcgta gccgagaatc tcacttgctt
    13621 catcaaatgt ctgctttaca acggcttctt catataaatc ttttcccata ccttgatatt
    13681 gtgcaccttg gccactaaat aagaatgctg ttttcatgtt tttctccaat atatccagaa
    13741 tatatgtttg attcttccta ttcaaaaagg ccgagacatt cctatttcag gaaatgtccc
    13801 agcccccaac ctaattttaa tgattatgca gtttgttttt ctacgtaagt cactaaatct
    13861 tgaaccgttt tgattccgtc ttctgtttcg attttcacgt caaattcatc ttcaatttca
    13921 ttgatgattt ggaataaatc caagctgtca gcttctaagt cttcttggat gtcagtagtc
    13981 aatttgattt cttcagtttc ttttcctaat tcttctgcga tgatttcttg aattttttcg
    14041 aataccataa taatatattc ctccgatatt gtttgctttt tatttttata atttaacagt
    14101 ctacaactcc atgagaatac ttccccatgt taaacctccg ccatagcctg taaatattac
    14161 tttctgctgt tttcccaagg tgatctttcc actagaaact gcttcatcca ataagatagg
    14221 gatgcttgca gcagatgtat tcccatattt atccatattg gtcaggaact tttcttgcgg
    14281 catctttact tttcgggcaa ttttctcaat aattctgagg tttgcctgat gcaaaagcag
    14341 ataatcaact ggtttgtctt tcatgacttc ttggatattg tttgcaacgt ctcgtacggc
    14401 aaaatcaaaa atatctctgc cagtcatttg caaataatac gaaccttctg aatcttctga
    14461 atagaacgga tttttatttt gctggtagcc agcggtcaat gagctacttc tggtaccgtc
    14521 agctgctaat ttttctttca gcaatttttt ctgtggattc gcttctaaga gaatcccacc
    14581 tgctccatcg ccaaacaaca cagcagtcgt acgatcagac caatcgatta gcttagatag
    14641 tgtttcacca ccaatcacta gtcccttagt tgagccgcag cggatcattt tttcagccat
    14701 gcttaacgca taaacaaatc ctgagcacgc agcactgata tcaaaagcga tagcatgagt
    14761 tgctcccaat ttcccttgta cttgtgctgc tacagatggc gtcgtgtaat cgggcgacat
    14821 tgttgctact aaaataaaat ccaaatcttt agggtttaga tttttctgtt ttatcaattt
    14881 ttccgccact aaaatgcata aatcagaagt ctcctgattt gttacacatc ttcgttcacg
    14941 aatacccgtc cttgacgaaa tccattcatc agaggtatcc atcatcttgg aaagatcatt
    15001 attagtaata atcttttcag gaaggacgct ggctgttgca gtaatcattc caaattgttc
    15061 cattaaatat cctcacttat agtcttgcaa gaagtcgtgc aagtttttca gtgcatccag
    15121 caaggcatgc tgttcctccg tagccattcc gtctaagatg ttcttgacca tttcacgatg
    15181 aaaatgctga tggacccgaa atagtaactt tccttttttc gttagtccca actttacaac
    15241 gcggcggtca tcttcactgc ggatacgttc gacatagcct ttttttacta acttgttaat
    15301 agcagtggtc aaggtcccaa cagtaattga tagttctttg gctacttctg aagttgtttt
    15361 tttcttgtac atcccgattg cctcgatcgt gtgcatctct gtgattgaca gatctttgaa
    15421 ttgagatttt ttcagctcag actcttcgat tgtcaaaata tcattgaaga cactaacaag
    15481 ataatcattt actgttttta aatctggttc catgttccac cgtccactaa ttagtttgat
    15541 tatcaaatct tttgatgttc aaactatatc ttaacttttt tcaaaatgca accttaaaag
    15601 atcatttttt tactatatga gcttttttta agattttact catttaaaac aactgataaa
    15661 tcagcattta taatttcttt tattag
//
LOCUS       DAXWLF010000053        15633 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXWLF010000053 DAXWLF010000000
VERSION     DAXWLF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.053"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..188)
                     /locus_tag="ACGE4U_001290"
     CDS             complement(<1..188)
                     /locus_tag="ACGE4U_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393466.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISER"
     gene            complement(201..764)
                     /locus_tag="ACGE4U_001291"
     CDS             complement(201..764)
                     /locus_tag="ACGE4U_001291"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFF8393467.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            992..1654
                     /locus_tag="ACGE4U_001292"
     CDS             992..1654
                     /locus_tag="ACGE4U_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFF8393468.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            1778..2974
                     /locus_tag="ACGE4U_001293"
     CDS             1778..2974
                     /locus_tag="ACGE4U_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFF8393469.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(3075..4451)
                     /locus_tag="ACGE4U_001294"
     CDS             complement(3075..4451)
                     /locus_tag="ACGE4U_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8393470.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(4488..5354)
                     /locus_tag="ACGE4U_001295"
     CDS             complement(4488..5354)
                     /locus_tag="ACGE4U_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFF8393471.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(5443..6468)
                     /locus_tag="ACGE4U_001296"
     CDS             complement(5443..6468)
                     /locus_tag="ACGE4U_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8393472.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(6486..7160)
                     /locus_tag="ACGE4U_001297"
     CDS             complement(6486..7160)
                     /locus_tag="ACGE4U_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8393473.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(7344..8237)
                     /locus_tag="ACGE4U_001298"
     CDS             complement(7344..8237)
                     /locus_tag="ACGE4U_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8393474.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(8311..10008)
                     /locus_tag="ACGE4U_001299"
     CDS             complement(8311..10008)
                     /locus_tag="ACGE4U_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8393475.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(10001..11314)
                     /locus_tag="ACGE4U_001300"
     CDS             complement(10001..11314)
                     /locus_tag="ACGE4U_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFF8393476.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(11314..15583)
                     /locus_tag="ACGE4U_001301"
                     /pseudo
     CDS             complement(11314..15583)
                     /locus_tag="ACGE4U_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
BASE COUNT         4414 a         3524 c         2708 g         4987 t
ORIGIN      
        1 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
       61 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
      121 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
      181 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
      241 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
      301 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
      361 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
      421 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
      481 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
      541 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
      601 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
      661 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
      721 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
      781 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
      841 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
      901 tctttatagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
      961 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
     1021 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
     1081 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
     1141 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
     1201 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
     1261 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
     1321 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
     1381 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
     1441 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
     1501 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
     1561 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
     1621 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
     1681 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
     1741 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
     1801 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
     1861 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
     1921 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
     1981 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
     2041 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
     2101 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
     2161 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
     2221 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
     2281 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
     2341 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
     2401 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
     2461 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
     2521 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
     2581 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
     2641 tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt
     2701 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
     2761 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
     2821 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
     2881 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
     2941 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
     3001 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
     3061 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
     3121 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
     3181 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
     3241 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
     3301 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
     3361 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
     3421 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
     3481 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
     3541 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
     3601 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
     3661 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
     3721 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
     3781 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
     3841 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
     3901 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
     3961 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
     4021 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
     4081 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
     4141 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
     4201 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
     4261 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
     4321 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
     4381 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
     4441 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
     4501 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
     4561 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
     4621 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
     4681 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
     4741 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
     4801 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
     4861 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
     4921 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
     4981 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
     5041 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
     5101 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
     5161 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
     5221 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
     5281 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
     5341 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
     5401 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
     5461 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
     5521 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
     5581 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
     5641 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
     5701 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
     5761 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
     5821 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
     5881 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
     5941 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
     6001 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
     6061 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
     6121 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
     6181 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
     6241 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
     6301 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
     6361 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
     6421 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
     6481 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
     6541 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
     6601 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
     6661 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
     6721 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
     6781 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
     6841 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
     6901 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
     6961 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
     7021 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
     7081 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
     7141 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
     7201 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
     7261 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
     7321 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
     7381 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
     7441 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
     7501 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
     7561 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
     7621 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
     7681 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
     7741 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
     7801 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
     7861 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
     7921 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
     7981 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
     8041 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
     8101 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
     8161 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
     8221 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
     8281 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
     8341 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
     8401 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
     8461 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
     8521 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
     8581 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
     8641 ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc
     8701 taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc
     8761 gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc
     8821 acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg
     8881 tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac
     8941 cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt
     9001 atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac
     9061 tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat
     9121 ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat
     9181 aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt
     9241 gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc
     9301 ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc
     9361 ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt
     9421 agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat
     9481 tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt
     9541 atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc
     9601 ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt
     9661 gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg
     9721 tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa
     9781 atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg
     9841 ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt
     9901 ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt
     9961 aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc
    10021 gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca
    10081 acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc
    10141 ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac
    10201 gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct
    10261 cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga
    10321 tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt
    10381 cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt
    10441 tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga
    10501 tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta
    10561 tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc
    10621 tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt ttaggataga
    10681 tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag
    10741 caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt
    10801 cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg
    10861 caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt
    10921 ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt
    10981 gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg
    11041 tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg
    11101 ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat
    11161 cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc
    11221 ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa
    11281 gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg
    11341 tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat
    11401 atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac
    11461 actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag
    11521 atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc
    11581 ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc
    11641 taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg
    11701 atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc
    11761 caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc
    11821 tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg
    11881 cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg
    11941 caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt
    12001 cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga
    12061 gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt
    12121 ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc
    12181 tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt
    12241 cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg
    12301 gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc
    12361 atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt
    12421 ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa
    12481 accttctggg ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact
    12541 atccttataa acaaatgaat ctggctctcc gattggatga ataactttct caccatccac
    12601 gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc
    12661 tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc
    12721 atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag
    12781 tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa
    12841 ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc
    12901 tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt
    12961 atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt
    13021 gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct
    13081 gatccaaaat atgtcgctga ctgctccagt ttcatctcct aaaatcggca tcttcgtttc
    13141 aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact
    13201 ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc
    13261 cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa
    13321 atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat
    13381 attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt
    13441 gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc
    13501 ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc
    13561 ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat ctagatggta
    13621 aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc
    13681 ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt
    13741 aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa
    13801 atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac
    13861 gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt
    13921 tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg
    13981 actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc
    14041 tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa
    14101 gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc
    14161 ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca
    14221 gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa
    14281 ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat tttctccgcc
    14341 attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc
    14401 gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt
    14461 caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta
    14521 aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat
    14581 ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa
    14641 ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt
    14701 tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg
    14761 tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc
    14821 gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg
    14881 agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta
    14941 atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc
    15001 atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac
    15061 ttttgtatag tcattcgttg taaacttcat ctattctttc atcaaccacc acttgaagag
    15121 gtagttaccc ttcgttcaca tcggggatct tttctgtttc aaaaatcaat gtgccggttt
    15181 ctgcttcttt tgaataaact tttccgacat accattcttg accatcgatc gtctcgactt
    15241 ttaggtcttc tccatcttca tcataggagg atagacctgt tccacgaacg ttttttacgg
    15301 taccaatccc ggcggctgcc gcatcgattg ctaaacgtat tctaccttca ttggcacttt
    15361 cctttttttc aaaagccaga tgccattcaa gggtttcttc tttttcaata tacttccctt
    15421 caatttttgc atagtcctca tctaagaata cctcatcctc ctttgtttct gttgactcag
    15481 cagctacatc cagggtcgtc atatgataga ctgactggaa atatggtcca aatagcaaac
    15541 tgacagttac taactgcaaa agcttcaatt ttaacccttt cacatcctta tactcctttc
    15601 aaaaatattt gtcttctttc acaacaatca aca
//
LOCUS       DAXWLF010000054        15251 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXWLF010000054 DAXWLF010000000
VERSION     DAXWLF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.054"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            42..1493
                     /locus_tag="ACGE4U_001302"
     CDS             42..1493
                     /locus_tag="ACGE4U_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFF8393477.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            1486..2208
                     /locus_tag="ACGE4U_001303"
     CDS             1486..2208
                     /locus_tag="ACGE4U_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8393478.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            2228..3379
                     /locus_tag="ACGE4U_001304"
     CDS             2228..3379
                     /locus_tag="ACGE4U_001304"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8393479.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(3727..4002)
                     /locus_tag="ACGE4U_001305"
     CDS             complement(3727..4002)
                     /locus_tag="ACGE4U_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFF8393480.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            4110..4874
                     /locus_tag="ACGE4U_001306"
     CDS             4110..4874
                     /locus_tag="ACGE4U_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFF8393481.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            4998..5501
                     /locus_tag="ACGE4U_001307"
     CDS             4998..5501
                     /locus_tag="ACGE4U_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8393482.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            5766..6413
                     /locus_tag="ACGE4U_001308"
     CDS             5766..6413
                     /locus_tag="ACGE4U_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFF8393483.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     regulatory      6456..6523
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            6574..7842
                     /locus_tag="ACGE4U_001309"
     CDS             6574..7842
                     /locus_tag="ACGE4U_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFF8393484.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(8019..9221)
                     /locus_tag="ACGE4U_001310"
     CDS             complement(8019..9221)
                     /locus_tag="ACGE4U_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFF8393485.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            9748..10473
                     /locus_tag="ACGE4U_001311"
     CDS             9748..10473
                     /locus_tag="ACGE4U_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFF8393486.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            10463..11137
                     /locus_tag="ACGE4U_001312"
     CDS             10463..11137
                     /locus_tag="ACGE4U_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFF8393487.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(11425..11709)
                     /locus_tag="ACGE4U_001313"
     CDS             complement(11425..11709)
                     /locus_tag="ACGE4U_001313"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFF8393488.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(12003..13310)
                     /locus_tag="ACGE4U_001314"
     CDS             complement(12003..13310)
                     /locus_tag="ACGE4U_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFF8393489.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            13799..14905
                     /locus_tag="ACGE4U_001315"
     CDS             13799..14905
                     /locus_tag="ACGE4U_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393490.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
BASE COUNT         4934 a         2627 c         3124 g         4566 t
ORIGIN      
        1 agcttttctg attacattat tattcaaagg tggccaaatg gatgaaaaaa ggaataaaca
       61 tattattagt ggcgctgatg tttctactgg tttttttacc agtaaaagta caaggtgcag
      121 aaaaagatga aatttacgag cggataaaac aaagtggaga attagtcgtt ggcctatctg
      181 cagactatgc gccctatgaa ttccatgcga atgtggatgg taaggataaa atcgttggtt
      241 ttgatgtatc cattgccgaa aagatcgcaa aggatatggg tgtgaaacta aaagtagaag
      301 aacttggttt tgatgcatta ttaggtgcgt tgaaaactgg caagatcgat ttagttatat
      361 ctggtatggc ggtgactgaa gaacgtttac aagaagttga cttttctgac ccatatttcg
      421 tggtacagca aaaagtactc gttcgtaaag aggacaaaaa taaatttaag acgacagccg
      481 acttttctgg cttagccgtt ggggcgcaaa agcaaaccac acaagaggag ttagtgaaaa
      541 atgaactgac tggtgcagag ttgacctccc tgcaaaaagt tcctgatctg atcaataatt
      601 tgctgaatga aaaagttgaa ggtgttgtgc tggaacaacc agttgcagaa gcatatgccc
      661 aacagaataa gaaagtagct ttgactccag taaaatttgc gcaaggcgaa aaaaccacag
      721 ctattgcgat gtcaaaaaat acaccagaac tgaaaaatca tgtcaatgcg tcaatcaaaa
      781 cgatcaatga cgacggcttg ctgaaagatt atcaaaaaca agcgaatcaa ttgatgttcc
      841 aagatgatca gtcctttctt caaaaatacg gtccgtatta tttgattgga acaaaataca
      901 ctattttatt agcttttgtg ggtgtattgt ttggaatggt ctttggtagt ttgcttgcac
      961 tgatgaaatt gggtaagaat aaatttttaa aggcattagc cattatttat atcgaatacg
     1021 tacggggaac acctctctta gttcaaatct ttatcgttta cttcggaaca ggagtactgg
     1081 gattggatct atcgaaactg gtagcaggtt gtattgctgt ctcgttgaat agtgctgctt
     1141 atgtagccga aattgtccgt gccgggatca atgcggtcaa caaaggacag atggaagctg
     1201 cacgttcttt aggaatggac cagaaagaag ccatgcgttt catcatcttc ccacaagcaa
     1261 tcaaaaatat tcttccagct ctaggaaatg agtttgtcac gatcatcaaa gagtcctcag
     1321 ttgtttcggt cattggggta tcggaattga tctttcaaac gggcgtggta caaggtgcta
     1381 gcttcaaacc attcctacca tatgtgttgg tctctttgat ttatttcgtg ttgaccttca
     1441 gtatttcccg tatattagct ttagttgaaa ggagaatggg tctgaatgct taatgtaaaa
     1501 aaactgtcaa aaagttttgg cgagaatgaa gtcttaaaat caattgatct ttctgtgaaa
     1561 gaaggagaag tagtggtgat cattggccct tcaggaagtg ggaaaagtac aattctacgt
     1621 tgtatgaatc tgctggaaga accaacttct ggtgaaatct tttttaaggg acaaaacatt
     1681 acagcccctg acagcgatat caatcaaatt cgtcaaaaaa tgggaatggt atttcaaagt
     1741 tttaatttgt ttccccatat gaatgtactc gaaaatttga ccatcacacc tgtaaaaatc
     1801 aaaaaagaag accctaaaaa agcaaaagaa catgctctat cattattgaa ccaagttggt
     1861 ctaaaggaaa aagtggacag ttatcctacc agtttatctg ggggacagca acaacgggta
     1921 gcaattgctc gagcattagc aatgaatcct gatgtgatgc ttttcgatga acctacatct
     1981 gcacttgatc ctgaaatggt cggtgaagtg ttggcagtca tgcaggattt agctaaaaaa
     2041 gggatgacga tggtcgtggt cactcatgaa atgggttttg ctaaagaagt tgccgatcgt
     2101 gtgattttta tggctgacgg catcattcaa gaagagggaa caccgaaaga gatttttgaa
     2161 aatccgcaaa atagtcgaac acaggatttc ttgaacaaag tgttgtaaca aagatggagg
     2221 aagtaagatg gacaaaagta agattgcacg ccggtttcag caaccagcag aaaatctgtt
     2281 gatgaatatt gctattttgg caaaggaaaa agaggatatt atcgatttat cgattggtga
     2341 tcctgacttg atcactgatc cgacgattat tgaagcagct tatacagatg taaaaaatgg
     2401 tgctactaat tatactgcgc ctgatggcag caaagaattc ttgtcaacgg tcgtagattt
     2461 ttatcaaaag aaatatggat tgagcgttga atgcaaccaa gttcgagcta cagtcggagc
     2521 aatgcacggg atgtatctag ttttacaggc attactagat ccagaggatg aggtcatcat
     2581 ccatgaacct tatttttcac catatcggga acaagtcatt ttatctggag gcaagcctgt
     2641 ttttgttcct acctatgaag aaaagggctt ccagttggat gtggaagatt taaaagcagc
     2701 gattacggat cgtacaaagg tgatcgtcat caattcgccg aataatccta cgggagctgt
     2761 tttttctgaa agcacgttga aaaaaatcgc tgaagtcgca atcgaaaatg aactctatat
     2821 catttccgat gagatttatg aagactttgc ctttaaagag aagttcgtac cgatggcgcg
     2881 atttgcgccc gataatacga tcactgtcag tggtgtatcg aaggggtttg cgatgactgg
     2941 ctggcgctta gggtacatga tcgcaccagc atacatcaat caaattgctg ggttgatcaa
     3001 tgaaaacatc acttattccg caccgactgc ttctcaaaga ggtgcaatct acgccttaaa
     3061 gcactatgaa cagcttgtac cgccagttgt tgaagtattc aaagaacgat tagcttatat
     3121 tgagaaaaga gtggcagaaa tcccttactt atctttatct tctgtttctg gaagcatgta
     3181 tgcttttatt aatatcagta agagtgggct cgattcagta acgtttgtgg aagaggtttt
     3241 agaaaaaagc ggcgtacttt ttgtccctgg aaaagcgttt ggggaaacga tcggggacaa
     3301 ctatattcga ctagcagcga ctcagccatt ggaaatctta ggtgaagcct ttgatcgttt
     3361 agcaaaacta aatttttaga taaaactgta tatttgaaaa cagctatggg tatccattag
     3421 aacaaagagg gaaaatgatc ctttcgagag ttggcttgtt gactgggcag cgtcaggatt
     3481 tgacaatgaa aaagtggtag tggaaaatca aagtcgattt tccactacca taaaatatta
     3541 tagactccat tttattgaac caaaattttt gaagtcctcc cgcttttacg tatgcctatc
     3601 actggttact aaactcgcta ataagaatgt ttttttgtcg atctaggatg gagagagggg
     3661 caaacttttt cgatctacat acagacagtc cgatttttct gacatttcct attcatttct
     3721 agaaggtcag cctattacaa caaacttttt tctgtcctcc atcatcggat tttcctgaag
     3781 gtctacatat tggacacgtc cgctggggct tggtgatcgt ttgacttctt ccacgaattt
     3841 tttgattgcc tcatcgtcgc ccgcagcttc gatcgtgaca gagccgtcat cttcattttt
     3901 cactgttccg gtgatcccta attgatccgc tacgattttt gtcatatagc gaaaaccaac
     3961 gctttgtaca cgtccttgca cgttcatctt gattttgttc attttattca ccgttctttc
     4021 ttttattttt aaattaatga taccgacttt tttgctactg aacaagtgca atgatctaat
     4081 ccatggtata atcagttgag gtgaaaaatg tgaaagagat ccaatcagca aaaaatccaa
     4141 gaatcaaaga attaaaaaaa ttacataaga aaaaatatcg tgaagaaaaa ggggaatatc
     4201 tgcttgaagg gtttcacctg atcgaggagg ctgttgcctc aaatgcgaag gtcaacgaac
     4261 tttttattac gcaacgcggt ctgactgagt gggctagttg gcttgaagaa aatcaggcgc
     4321 tttctgttac actagtatca gaagaagtga tggcaactgt ttcagatcta ccaactccgc
     4381 aagggatcat ggcagtggtg gaagctgaaa aaagagagat accgaatttt gaaggtggtt
     4441 ggttgttgct cgacaacgta caagatcccg gcaatgtagg gacaatgatc cgcacagccg
     4501 atgcttttgg tctatcttgt gttgtactag gtaagggaac ggcggatgtc tacagtacca
     4561 aagtattaag aagcatgcag gggagtaact atcatttacc gatcatccaa gaggatctaa
     4621 caaaactatt gcctatcctt aaagagcaag gatttataac ctatggaaca gaactgaatg
     4681 aagaagcagt gtccctgaat gatatccaga caacagaaaa ttatgcgatc gtgatgggga
     4741 atgaaggtca aggcgtacgt ccagaagtgc tggcgctaac ggataaaaat gtgtatatac
     4801 cgattcaagg aaatgcggag tctttaaatg taggagttgc agcgggcatc atgctttatc
     4861 acttctccgt atagttaagg aactattaag ctttctgcat tttgcgaaaa cctcactaga
     4921 atattacgaa cactagtggt atactaagtt caatttcaaa gataaatgga attcaaaaag
     4981 gtggcagatc gataattatg acagaaaaat ggcgtgaaga cgaagaatat atgtcttata
     5041 taaatgatct attagaaact gatgcagtaa agaaattagc aaattacacg caacatgtcc
     5101 actcgacacg attagagcat tcaatcagtg tgtcttacta tagctacaaa ttagcgaaaa
     5161 aatggggcgg tgatgcaaga gcgactgctc gagcaggcct tttacatgat ttattttact
     5221 acgattggcg tacgacgaag ttcgatgaag gtacacatgc gtatgttcac ccaaggatcg
     5281 ctgtgaaaaa tgctgaaaaa atcacggagc tttcagatct agagcgtgat attattttga
     5341 aacatatgtg gggagcaacg attgcccctc ctaaatataa agaaggttat atcgttactt
     5401 ttgtggataa atattgtgca gtcaaagaag cagctcaacc aatgacagct tcaatgaaac
     5461 aaaaatggca acgatatttc ggtaaagaac aatcgattta aggtagttaa ctaagtattt
     5521 gacccgagta ttttgaaaaa aatcctcggg ttttttgttt agacattgac taattggtct
     5581 ttttagtgtt tactttgttg agatgaaaaa gtacataacc aacataaaaa taaagggggt
     5641 gatgataaga tgcgatgtgt atcttattta aaagagtttg agggctttat ctaaaggcac
     5701 tttgatagaa agatcacttt tttcttcaaa gtagccgatt ttgaaagaag aaaggactca
     5761 aagtcatgga aaaaacaatc aaaccatatc aatattattt tatcaagcga gaagtcgagc
     5821 agctgttaag cgcctatagt tcagtgaatg atccaaagac agtccaaaca gtccaagcat
     5881 tagctgcgga aaagatccac gaggtacttg gtcatcctaa tacagctgta aatgaattta
     5941 tggaaaaggt actagacgtc actctaaaaa aagaaaaagc agaacgacta ttagaggaat
     6001 tgaaagtgat cgttcttcca ttcatacaac cgacaaaacc gcagatcgat aaattattcc
     6061 gaaaagtgaa aaagttaaaa caaccagatt ggaatcaact ggatttaagg gaacatactt
     6121 atattggttg gaatgatcca ggtagccaaa aaaaatttat gatctattat gaagaagaaa
     6181 aactaaaagg gatcagtgga acattatctc ctacaatcaa caaaggaatt tgttcgattt
     6241 gtcaaagaac ttcaaatgtt tcactctttc tttctaccac aaaaactggt tcagatggaa
     6301 catacacgaa aaaaggaaat tatatttgtc atgatagtga ccaatgtaat cagcagttgg
     6361 tacaattagc tcctttttac gaattcacag aacgagtgaa acgtgagcgg tgatttcatt
     6421 gtatttttct gaaaaatatg ctatcttatt tggtgaagtg aataggtgat ggtgttcgcc
     6481 tttaaacgtt gatcgtaaga taactaatga cgcctactaa agaaacgggg ctaccccttt
     6541 ttttagtagg cgtttttttg aggaggaaat gagatggaaa atcgattacc tgatccattg
     6601 aaggtgatca tctatatgat caaatggtta ttgatcacag caattttagg tatttttatg
     6661 ggaagtttat ccgcattctt tctaactagt ttgactgatg tgacagctat tcggctggag
     6721 catccttggt tactttttct tttgccgata agtgggggac tttttgctta tctatatacg
     6781 acgtatgggg gcaattcttc ccggggaaac aatttagtga tcgatcaagg gaatggagga
     6841 aatgaaaaga ttccattacg gctgatccca ctgaccttat ttggcaccat taccacgcat
     6901 ctttttggtg gttctgttgg tcgggagggt acagcggtcc aaatgggcgg ggcacttgct
     6961 gagaattttg ctcgttggct aaaattgaac aaaatcgagc gtgagattct tgtcatcagt
     7021 gggatcagcg caggttttag ctcagtattt gggacaccct tggctgggac attgtttgga
     7081 ttagaagtat tggcaatcgg aaaagtgaga accgaagcac tgtttccaag cttttttgca
     7141 gcactttttg cgaactttgt aacggaaagt ttcaatgtca agcataccca ttatcaaatg
     7201 ggggcgattc ctgcatggga cccttcactt ttcttcaaat tgttgcttgc gggcattgca
     7261 tttggtatca ttggctggct ctttagccgt tcgatcgtct ggttaaaagc ttggtatgca
     7321 aagcggatcc cccatccagt tttacgcaac tttattggag gagcagtcgt ggtactcgta
     7381 gtagtgattt tacagacgca aagatatctc ggattaagct tacctttatt acaagatgca
     7441 tttaatggaa gtgctcatcc ttatgatttt cttgggaaac ttggctttac cgttctatca
     7501 cttggcgcgg gataccaagg aggcgaagtg acccctttgt ttgagattgg ggcaacttta
     7561 ggtgcgacat tagcacccat tcttcatgta tcagtacctt ttttagcagg attagggttt
     7621 attggggtgt tctctggagc aacgaataca ccgattgctt gctttgttat ggggatcgaa
     7681 ctttttggag gtcaagcagc cagctacttc tttatgatct gtttgattag ctttatgtgt
     7741 tcaggaaata gtggaatcta ttctgcgcaa aaagttgaag tgaaaaaagg aatcttgttc
     7801 ctaccgatcc ttggaagaat ctcacgacaa aaagagaaat aaaaagaggt tgggacagaa
     7861 gtgtttaact ccgagaaata agaagaaatt cacgaaaatt gctttttaaa tttttgtgaa
     7921 tttcgcttat ttccgaagga gttgcttctg ctcccaccgt ttattcggag ttagagtctg
     7981 tggcaaaact gatttttagt tttgtcccac gctctttttt attgagcaaa tttgagaata
     8041 agagcagaaa atactgcaaa gccaatcatt aagccacaga tgataagatg gcgttttttg
     8101 tgtaagacaa ggatggcaat aaattctctt aagatagcat tcggtaagta gtatagtgca
     8161 gtttttgcac cgattccgtt tgcattgagt ttcgcttgtc gcgcatatag tccagctcta
     8221 aagatatggt aattatttga agtaaaaatc gcgcgataag gcccattcat ttgctgatcc
     8281 attatttctt ttgaaaacag catattttct agcgtcgttg tggaattcgt ttctactaaa
     8341 atatcacgct cgggaatccc tttttctaaa gcatattgtt tcattgcaat ggcttcagct
     8401 actttttcat cggttccttt tcctcctgac atgagcaaga ctggcggatt caaagttgct
     8461 ttactttgtg cccaataaaa tgcgatcgcc ttattgatcc ttttagcaag taggggagat
     8521 acgcgttcac catcaatcaa gccagcgccg agaacaacga tataatcttg gttatacctt
     8581 ggacggttga actgatacaa aattgaaaca gtcataaaat taaagaaaac gatgagtaga
     8641 taagcaaaaa taaacggaat gacagcaaat agagcatttg cccattgtgg gagccaatgg
     8701 actaatgtat agtccaatat caaaaagata gtcaaagcga ctcctaacaa gagagtcaaa
     8761 aggttggcca aagaacggga ttcttttttc atgactacaa caccattcca aaataaaaaa
     8821 atgatcaaaa tgtagatacc gattgccaaa agaattaata ttggtatccc gatcaaagcc
     8881 aataacccac cgataaagat attttgcgtc tctattagta gccaccctag ataaccacca
     8941 aagacaatca aaaacgtatt aaaaagtacg ccattgatta acctacgttt ttccttaaaa
     9001 tagaaaaatg caaaaatact gaaaaatagc aatggaatca aaaaataaaa tgtatgataa
     9061 aaaacttcta aaacgacgaa gtaagtgacc atcatcataa aagtccacgt cccacaataa
     9121 aagaatagag cgggatcgct ttggcgcttg cgccaataga ggatgcctga cattcctaaa
     9181 atatagataa ctaaaaatat caggtctcta gtaagattca ttgaatgaac tcctttcgat
     9241 ttgtaaattt tttcttaaaa aagatagcat catttagtag taaatatatc gaatatagaa
     9301 aagttagtta ctaaatagtc aatcagtttt ttcacgacgt ttctattata acgaattatg
     9361 ttgaaaaaag cttttatcga aaacataaat cttcaaatta acagattttt tagacctcga
     9421 tcatgatgaa gacttttgta gatacaaata actgtaacat tgaaaatgaa tgcgaaaacg
     9481 atattttttt ttattggaaa atatcggtca agcaataaac cagttgcata ataaaatgtt
     9541 aacggcaaca aaatctgtgg aaggaatgaa ccggatgatt tgatggaaaa aacaagatag
     9601 acaagaagta tacatagtaa aacgtggaac aagtaggcca gaatgaaaaa tcagttttta
     9661 tccactcgct tttctataca ttcactgtca caaatataat cgaaagtaat tctattttga
     9721 gataaagtat tttataggag atagtatatg aacaaaaaaa gatttaaacg tattgaaaga
     9781 aaatttgcat taacttatga ccaagctaag tatttgaaaa cactatttcc aaaacaggta
     9841 gttcgggata aatatagtga tcattttatt tcttccttgt attttgatac aaacgatcat
     9901 cactttttaa aagaaatcac tacagaaaag tcctatcgag aaaaatttag aatcagaaaa
     9961 tatggagatc ctcaggacat atcgcctatc tacttagaga taaaaagaaa gtttcaagga
    10021 atgacagata aacgtagaat aaaattagcg catcatgagc tcgatgatat gttggaaaat
    10081 atcgaattgg cagaaaccac attcagtaat ttagaagaag agtatatttt taatgaaata
    10141 cagtggttgt tcagaaggtt ggcattggag cccaaagtat atatctatta tttaagagaa
    10201 gcatttgtaa atagagattc agaagatttg cgaatcactt ttgatcaaaa tatccattac
    10261 cgtctaatga acttaacgac ctgggaacac tgtacagaag acaatctggt agcgccagaa
    10321 atcgatgtgc tgatggaagt aaaaacgaca ggagaaatac ctgaatggtt acaggtagga
    10381 ttggatagga tgggtaaaaa agagataaaa ttttctaaat ataaacaagt ctataaaaga
    10441 tatatccaag aggacccgct ctatgttcaa tagtgtctta aaaaatgagc cttctcaact
    10501 cacactagat gatttctttt tatgtatggt ggtgtcgatt ttgctaggca ttctgattgc
    10561 ttacacccat atgtatcaaa acaactgtac aaaaaacttc atgtttgcct tgattgggtt
    10621 acctacttta gtacataccg tcattttgtt gatcaacggt aacttaggca cggggatcgc
    10681 tgttctaggc gcatttaact tagtgcgatt tcggtctgct caagggaatt caagagatat
    10741 tttcagtatt ttttgggcaa cgtcgattgg tttagccaca gggacgggat atattgtcta
    10801 tgcactattc gtgtctagta tagttgctgc tatccaagtc atcatcttca cgcttccaat
    10861 caaaaataaa catacagttt ttaatactga aaaaacgatc aagctaaaag tttctaaatc
    10921 cttgtttctt tcgagtatta ttgaagaaaa aattgcagaa tatacagaaa actgcaattt
    10981 aaaaaaagta aaaatcaata aagaaacttg ttccttacac tatatagtaa aagtaaaaac
    11041 gagtatgctt gaaaaagaat tggttcgaac gatcatggat catccagaag tcatttcgat
    11101 cactgtaggt atggataaat cgaataaatt attgtgataa ccaagaaagt acttaacaat
    11161 gaagtaagac cccagttcca aagtttagcg gggacacttc aataaagtac tttctttttt
    11221 gtcttcagca actactggta aaactgagag caagctttcg tgagagtaca gcgataaaga
    11281 tcaacctccc tttttatctc gagcaatttc cttgcaaggc tgtttcgttg atcttaaacg
    11341 agttagcaac agtcaaagat gcgatggcca taaaaaaatt gcattaagtt ggctggtatc
    11401 tgatagctgg gaatctaagg aagactatcc attctgtttc ttaaattccg ctagttgata
    11461 gatatcagcc aattgttcca gatgatcaac atcaaagata accgaaagat gtttcctttt
    11521 attcccattt gcttgaagag tttttatctt ctctttggtt ccttcactca atgagagtgt
    11581 gactaatttc tccctcgttt tttctgggaa atgaaaggat actttgaaaa atccgtccca
    11641 aatagaaagc cagaagatcg gttttacttt ctgagagtgg gacaaaagta agaaacactt
    11701 ttgtctcatt ctttaaaaac gaataaacgg tgggagcaga agcaactcct tcggaaataa
    11761 gccaaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttctcggagt
    11821 taaacacttc tgtcccaacc tcttgtattc accttttgat agccaagctt ttccatcatt
    11881 ataaaaccgc aaatttcaaa aaaaaacgaa caatacaaaa agcgtattgc tcgtttttct
    11941 cttcagtttc tcacacctaa acgacttttg tctcgacctc tttttggtga ctattcatga
    12001 tgttaaaata aattactgaa aaaatctgtg acatggcgcc aaccaatgac aaaaatatta
    12061 gctttttcaa cagtagaagt ggtaatgatg tctgtcgaag gctcttggtt tgcttccaaa
    12121 tagcctaatt tgtcatcagc taattggatc gttgctgagc caactgtagt gcctttgttg
    12181 ataggagcgg tcacttggtc atccgttact ttctttttat caattgttgg agtaatcgtt
    12241 aattttccag tatccattcc attttcgacc cagactttta cagggctttt tagcgcaaca
    12301 ttgacggagg tttcttttcc atctttcact ttgatgtctt ttaggcttgg gatgcttgca
    12361 tttgcttttc caagttcttt ttctgaccag ttgtcataac aataatccat caatcgtgat
    12421 gtttcattga atcgggcact tggattttct tcatggttat ctgcatttaa aacgactgtg
    12481 atgattcgtc ggccatcttt gttgatcgtt ccgacaaagc atgcacccgc aaaatcagtt
    12541 gtgccagttt ttaatccatc gacaccttcg tgatagttta caaaaccagg tagcatccag
    12601 ttccaattga ccatttcaac cggtgactgt gtgttttcgc cgaatgtttg atttgtggta
    12661 ctcgtgattt ttaagacttc aggataatct aagatgaggt gacgagcaac aatagcgaca
    12721 tctttcgcag acagttgatt ttcatctgtt tcaccagtat tctcaggtcg attctcacct
    12781 aggtaagaat tgtttaaacc tgatgcattg acgatcgttg catctttgat accccaatct
    12841 tttagttgtt tcttcattag ctcaagaaat ttactttcac tgcctgcaac tttttcagct
    12901 aaagcaacca tggaagcatt ggctgattgg atgaaagctg agtcaacaag ttctttcact
    12961 gtataatcat tttctttatg caaaggaaca ttcgatagat ccggcacgac acttaatttt
    13021 tctgcataat ccgaaatggc taccttatct tcccaagata atttaccctc tttgatttgt
    13081 tcaagtacga tatatagacc aatgatcttt gtgatggaag caattcccat cgttttttcg
    13141 ccatcctgat cataaaggat ttttccactt tctgcatcta cggcaaaggc agctttggca
    13201 tttattttaa atgcgtcgtc tgcttgaaca gttgcgctag gcaagaaaga agtgatacat
    13261 aagataagac ttgctaatag tacaataaaa cgggtatttt ttttgaacat ctttgttctc
    13321 ctcattttta aattgattct tacaacttag aatattatca gagagaacaa aaggtttcaa
    13381 tggttctgaa aaaaacctta taaagaacaa gtcttattaa tgaagaatca tagaaagtta
    13441 tcttttgggt tcattgacac gaactttgac tggctgaact tcgggttcgg ttaggcgtga
    13501 tgctgcaaaa agggcgcctg ctgagagtgc gaccaaacta aaaatcatga ataattgcat
    13561 aagatcacct cgttcttttt tgttttttta tagtatagca agagagtttg aagctactgt
    13621 taaggaaatg agcatataat tgatgaaagt atgaaaaaaa tatgaagtag gacaacacaa
    13681 ctaaaaatcg atcaggtatt tctctggtag ggattcgttt tattgtattg ataaaatggg
    13741 atgtggtaga ctttatttac agatacaaag aaatgatttt tttaggaggg gtacgtttat
    13801 ggcaaaaaaa gaaaaagaat tattatcaca attgacttca gaaggtacca aaggaccgtt
    13861 tgtcacgatt atgttaaaca cccatgttgc gcatcaagac gtagaaaaag atcaaatcaa
    13921 attcaagaat tttgcaaagg aagcgaaaaa gcgctttgaa aagaaatact ctgagcatga
    13981 ttggtctcca ttccaagaaa aaattactag tttgttggac gatccgtctt tctggcgtag
    14041 tgccacaacg agtgtagctg tcatagtatc accagaacaa gtgtttatcc accgtttggg
    14101 tattcctgta gatgaccagt attatgtgag tgatctacct tatttattag cgatcatcaa
    14161 aaatggacat ttcaactatt catactattt attaggattg aatcgtgatt cgatgaaatt
    14221 gtacgaagta acgaataaac aagtcaaaga agtggctttg cctgaagatg caccaagaga
    14281 tgtcgtcact gctttaggat ctgaactaac aggaggcggg ttgaactacg catcaaatgg
    14341 tagtgaaggt acgacgttcc atggagtcaa tcctaaagat gaagaagtcg aaattgattg
    14401 ggtcaattat tatcaagcag tcgatgacta tttgaaagat acatttgaaa atccagagaa
    14461 atggcctatc ttcttatttg cattaccaga aaatcagacg atgttcaaaa aagtggcgaa
    14521 gaacccttat tatcgttccg atgttgcgat ctcagcttca cctgcacaac tttcattaaa
    14581 agaaattgaa gcaggcgcaa aaaaactatc gcaagaatta gctgtcaaag aagcaagtga
    14641 ctacaataag ttgcttgata aaaagttcat tgatcaactc gttgatatta taccggcagc
    14701 aaaagaaggg aaaatatccc atttattcat tgctacatct aatttagcag atggttttgg
    14761 cgaagatcca gaaacagaat atgaccgccg tcaggtgctg aatgaactag cagataatgt
    14821 gatcacgaac ggtggtcagg tctttatcct tgaacaaaaa gatgcaccag acgaaaagag
    14881 tttagttgcg atcttacgtt attgagtgaa aactaccaaa gtagatgaat ggtcaggcgt
    14941 tgatagaagt gtatagctcc gagagaagga gtttcctctg tttcagccgt tggattcatc
    15001 ttgagcgaga gacaaaagct agcttttgtc tctcgctcat tatttttgtg ctttagtccc
    15061 attggctttg cgaggggaag atacttttct caaaaaaacg aaccttgcag ttgagagggg
    15121 gatagatgcg catactaatt tttatcagtt aaaaaatgat gtggatcatt agagaattcc
    15181 aaagttcgtt catccttttt cagataataa attgaaattt tttgcaaaag tgaaaatagc
    15241 aaatagaaat t
//
LOCUS       DAXWLF010000055        15081 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXWLF010000055 DAXWLF010000000
VERSION     DAXWLF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.055"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACGE4U_001316"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACGE4U_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFF8393491.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACGE4U_001317"
     CDS             complement(322..891)
                     /locus_tag="ACGE4U_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFF8393492.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACGE4U_001318"
     CDS             1077..1979
                     /locus_tag="ACGE4U_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8393493.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACGE4U_001319"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACGE4U_001319"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFF8393494.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACGE4U_001320"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACGE4U_001320"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFF8393495.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACGE4U_001321"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACGE4U_001321"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFF8393496.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACGE4U_001322"
     CDS             8547..8732
                     /locus_tag="ACGE4U_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFF8393497.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACGE4U_001323"
     CDS             complement(8794..9990)
                     /locus_tag="ACGE4U_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFF8393498.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACGE4U_001324"
     CDS             complement(10160..11596)
                     /locus_tag="ACGE4U_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFF8393499.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACGE4U_001325"
     CDS             11889..12698
                     /locus_tag="ACGE4U_001325"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8393500.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12882..15035)
                     /locus_tag="ACGE4U_001326"
     CDS             complement(12882..15035)
                     /locus_tag="ACGE4U_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFF8393501.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDRELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4402 a         3200 c         2664 g         4815 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aaggttgtga caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag
    12781 tttctcggta tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt
    12841 cttcatactc gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata
    12901 tcataatacg cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct
    12961 aataagttcg gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc
    13021 acgatattac tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc
    13081 cctgtttttg cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga
    13141 tcggtaccat ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa
    13201 atattgaccg tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca
    13261 ttttcgtcat tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta
    13321 gcgactgttg aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat
    13381 gccaaatcgg taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc
    13441 ttcatataat caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc
    13501 gttgtaccaa gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct
    13561 tcatctaaac taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc
    13621 actttgatca tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact
    13681 tgtccgcttc gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca
    13741 tcaatcaaaa cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca
    13801 gccttgacta cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg
    13861 atttctttgg aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga
    13921 ggattcatag ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat
    13981 gtttgataat tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat
    14041 aacattaaat tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct
    14101 tcattatcaa gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt
    14161 ttttccaaat aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc
    14221 tctgcttcat ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc
    14281 agtgaacttg ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt
    14341 tcagaagcgc gttctgccac aacagctaac tctcgatcag taacaccaga gtttttgata
    14401 tagacagtgt ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat
    14461 tgctcctcgt ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt
    14521 tcgctgttcc ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt
    14581 ttgggatcag ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga
    14641 acatcgatca gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt
    14701 gttcctctcg tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg
    14761 gagtcgtaga tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc
    14821 actcgcgcct ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac
    14881 gcaacaaata acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac
    14941 cggcgaaacg ttttgttctt aggttcttgt gcaccttttc ttattttttc taaccaagaa
    15001 gtatttaata tttttttcat aaaatctttc atcatttcct atccttccgc ctatttacat
    15061 tcatgcttca ttgtacccaa a
//
LOCUS       DAXWLF010000056        15041 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXWLF010000056 DAXWLF010000000
VERSION     DAXWLF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.056"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(644..985)
                     /locus_tag="ACGE4U_001327"
     CDS             complement(644..985)
                     /locus_tag="ACGE4U_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8393502.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(985..1215)
                     /locus_tag="ACGE4U_001328"
     CDS             complement(985..1215)
                     /locus_tag="ACGE4U_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8393503.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1502..1840
                     /locus_tag="ACGE4U_001329"
     CDS             1502..1840
                     /locus_tag="ACGE4U_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393504.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2045..2607)
                     /locus_tag="ACGE4U_001330"
                     /pseudo
     CDS             complement(2045..2607)
                     /locus_tag="ACGE4U_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3339..3920)
                     /locus_tag="ACGE4U_001331"
     CDS             complement(3339..3920)
                     /locus_tag="ACGE4U_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8393505.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4103..4729)
                     /locus_tag="ACGE4U_001332"
     CDS             complement(4103..4729)
                     /locus_tag="ACGE4U_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFF8393506.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4751..6082)
                     /locus_tag="ACGE4U_001333"
     CDS             complement(4751..6082)
                     /locus_tag="ACGE4U_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFF8393507.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6237..6638)
                     /locus_tag="ACGE4U_001334"
     CDS             complement(6237..6638)
                     /locus_tag="ACGE4U_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFF8393508.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6724..6930)
                     /locus_tag="ACGE4U_001335"
     CDS             complement(6724..6930)
                     /locus_tag="ACGE4U_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393509.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7025..8527)
                     /locus_tag="ACGE4U_001336"
     CDS             complement(7025..8527)
                     /locus_tag="ACGE4U_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFF8393510.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8545..9000)
                     /gene="argR"
                     /locus_tag="ACGE4U_001337"
     CDS             complement(8545..9000)
                     /gene="argR"
                     /locus_tag="ACGE4U_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8393511.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9098..9715)
                     /locus_tag="ACGE4U_001338"
     CDS             complement(9098..9715)
                     /locus_tag="ACGE4U_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8393512.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9936..11309
                     /locus_tag="ACGE4U_001339"
     CDS             9936..11309
                     /locus_tag="ACGE4U_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8393513.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11384..12496
                     /locus_tag="ACGE4U_001340"
     CDS             11384..12496
                     /locus_tag="ACGE4U_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8393514.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12568..14205)
                     /locus_tag="ACGE4U_001341"
     CDS             complement(12568..14205)
                     /locus_tag="ACGE4U_001341"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFF8393515.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14373..14981)
                     /locus_tag="ACGE4U_001342"
     CDS             complement(14373..14981)
                     /locus_tag="ACGE4U_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8393516.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4106 a         3111 c         2639 g         5185 t
ORIGIN      
        1 actaatcata aaaggtactt ttacattcat aattgtcgct ttgagattct actttggttc
       61 actatctgac ccaattgcac attttcgatc attacaagta ttggtgtcgt tagaacaact
      121 aaataaaaac tagcggtaga tttttcagaa tctatgctaa tttctgtatg gatgttccag
      181 tgatggcaaa tttctctttt cattgttttt cttgtcgcat gatatgcctt tttgtgttat
      241 tcagttaaac tatagaaatt ctttcctaga cagatcttga tcccaaattt tacgtttttg
      301 agcatcgata tagtttaaaa tgacattact catatctaaa gagtttagat tttttaggac
      361 taaaactgtc agttgttctt tttcttcttt ctgttaaagt attgtcgcga cttttcgtat
      421 acgttaagat atgtcgtgaa aaaacaagtt ttgtgacaca aaaaaacgaa tgacttgagg
      481 aaatcacctc aatcattcgt ttaaaatgct atatattctt tagtttaaga tacggaaatt
      541 aatgccatcg accacactag tagaatgaac gacatttact tggccttttt ttaatttcgt
      601 tatgtttagc atataaaaat attatagagt ttacttacaa tgcttaaaac atatactgta
      661 taatctgatc aattttagcc atgtcttgca atggtaagtt ttctattttt ttgagttttc
      721 gttccttaaa atcaagagat tttaattgtg taataattac ttgtcctttt atatctagct
      781 cttcaggtaa tgagtaacgt gtcggtaaat tctttatagt ggaagtaatt ggacatatga
      841 ccgaaaacat tgttttacgg ttaaattcat aacgagagac gactagtcct ggtcgtcttt
      901 tttgaatttc ttttcctacg gagggatcaa aatcaatcca tacaatatca ccttttttag
      961 gaatataaat tctttcttct gacactataa aatttctctc ccttcaggag ttaaatcttc
     1021 ccattcgtct ttctcataat actcggcctc ttccccacca ctaaatggat cttcaatttt
     1081 tggaactaac gtaattgttc catcatcaga atacacaacg atatattctt ggttttctga
     1141 aggtttcttt ccgttatcag aaggtaaagt taccactaca gaacttcctt gtaatctaga
     1201 ttttgtaaca agcattatca tcactccctt aataaaagta tactatataa cactaagtaa
     1261 cgcaaaattc ccctttgtaa actatctaga aaattgtttt gtttaaagta tatcttattg
     1321 gatataaata gaaataaatt gtagccagtt cattttttct ttaactaaaa aaataatcca
     1381 agaaacattg aacctatgga tccaaaagct acccaaatac tatagctaac cccccgctac
     1441 cttcaatttt gcagataaaa cgctttggga gaaaatttca aagacaacag gccatcctat
     1501 catgcccgat tataaacaaa aagctggcta ttccttttgc tctgacaaat ttcaattggg
     1561 aaagttcacg aaactagcag gtgaagaagt ttcaactgtt cggatcaaag aatgcccgct
     1621 tcaaatagaa acattggttc aacgaatttc aactagacaa gactttgcta ttgtagaacg
     1681 tgagatcgta gctatttttg tagaagaagg gattttacat gatccaactc atatcaatgt
     1741 ggataaatgg caacctctta tctataaatt cagagaatat acaactgcca accagtcgtt
     1801 aactttgaat tttagattcc aagaattcaa agagtcataa tgataaacat cgttaggtat
     1861 aaataaaaaa gactcgcagc acgagtaaaa aacaaataga agctatacat tagaaaggtc
     1921 gactgtgacc ctatagatgc agtatatgca gcttatgagt atttcttctg ttctctttgt
     1981 ttttgggatc tgtgctgaag tctttttcgt tttttgccgc tctttctttg aaaaatcgat
     2041 tatgttatac cttctccatt actaattcag gcatctgctg agtaatgatt tttcgcgcaa
     2101 aagaaatatc tgttacccaa ggttcgattt gttcttcagt caagacaagc ggcattcggt
     2161 catggattgg tgcgactgtg cggttaggtt cagtagtcat tatgattgat tctgccattt
     2221 tttcatcctg atggctacga taaaaaccgc caatatacac tattttttcc ttatttgaaa
     2281 acataaattt gtctttttct tcattccact cattgggaaa acacaacgat tttgcaaaaa
     2341 tggttgatga aaggtctttt tttcttctac tgtctctgca cgggcattaa tcatcaattg
     2401 tcctttttta aatccggtaa atccccactt catgatttcc ggagtgagga tgttttcttc
     2461 ctcttttctt aacgtcacaa caagatttga cggaaaaact tctccagctg ctaattctgc
     2521 tgcctgcttc ggttgtttcg tctgggcttc tcgataataa ttctgaagga gctctgactc
     2581 tagatcaaaa aaataacgac cacacatcct aatccctccc aatctttttt tctatcatac
     2641 actgcaacaa gaagttcgca ccaacaaaac gctttcaatt tttgtagaac taaaaaagtt
     2701 gtgacaagtg acaaactctc cagtgaatgg tattctcctg ccaactcctc ggtcacaagt
     2761 cacaatattc aacaaacatg agaagctgct ttttgaaatt gttccttgtg actcggagtg
     2821 aaacggcagg cttacgacct ctgggtaaac ggtgttcaaa agccagtttc tcggtattaa
     2881 gccaaattta tgcaaaaatg tagaaagcta ttttcacaaa atttttctta atactcgaaa
     2941 ctaagcgact ttttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta
     3001 ataagcccaa ccaaacgaaa aatatgagca gctattttta tttggctgtt cttattactt
     3061 gatgcagggc agctttttca caacctctcc actaaacgat gttcaatcag ctaaccttcg
     3121 gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac
     3181 tcaggtcaaa gcgctgattt cacaacctct aatacacggt gttccagaag tagcttcttc
     3241 gaaaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
     3301 ttcttgaagc tgaccacttc tgtcacaacc tctaattatt aaagaacctt ttgcgaagtt
     3361 aacaatcttt ctttgatcaa ttgaaaattc tgcctaagta gtgatagatc ctgtgtgact
     3421 ttcaacaata agatacttcc ctggatttca gcaatcaacg ctttggcttg acggtctgct
     3481 tcttcatcgg acagtttttc ttcagaaagt gccaggatat tttgtttcgt atacttgacc
     3541 cattgattga ctaattctag tagcaacaga cggtattctt catccactgt actcaactca
     3601 ataatcaaat tacctacagg acagccatga taagtgatct gtgtctcatg atcagcgaaa
     3661 atccagtcca acatttcttc taatgcttct tctggctgtt ttccagcatt taagatgtct
     3721 tcgaacaatt ctgtctgcca tacgtccata tgcgcatgga tcaccgctag acaaacttct
     3781 ttttttgaag aaaagtaata ataaaattga ccttttcctg tatcagaagc tgctagaata
     3841 tctcggattg acgtttcatg atagcctttt ttataaataa gatctttggt cgcttccaag
     3901 atcttttcct ttgttttcaa tgatattctc ctttttcatt tctcatagtc ctatattata
     3961 cagtaaattc atcatttttt cttcttaatc gtttaaaaaa gactgtgcca aattatctga
     4021 cacagccctc actataaaac tgttctcata tagctagatg ttcattgatt caagaactgc
     4081 gaggacaaaa gcaattccta tcttatgata gttgctctaa tacttcctct gttgtcatca
     4141 cttgatctgc aataaagcca aagttcgtca acgctgcatc cattgcttct tggccaggag
     4201 caccaattgc atctgtcgct accacaacat tgaatccact ctctaccaat tcacgcatat
     4261 gggaatcgct acacaagttt ccgttcattc caccaagaat tactgtgtcg attccatttt
     4321 ttctcaattg gagcgccaaa tcattgcttt ctggtccaaa gattttatga ggagagctga
     4381 tgatcgtatt ttcatccaaa taaggtttca attcttcgat aaaatcggct cctgaacctt
     4441 cttccactga ttcatattga ctttttctag caaacatctg ctgctcaagc atcatatgtt
     4501 ctcctgcacc gccaaattgc catttatggt catgtgggta aaaataatga ggagaaataa
     4561 agagtttgta tcctttttct tttgcttgtt ttaccatttc aatcatattt tcgatcgtat
     4621 tgtatttttc taagatatct tttgtcaaac caaagccacc gcctgctggt ttcaagaact
     4681 cgacttgcgg gtcagtgaga actactgctg tatttttatt attgaacatg gtggattctc
     4741 cattcgttat ttattttaat acatcattta gagcttcact cattgtcgga tgagaataga
     4801 tttgatctct caataacgta taaggaagtt ttccattcat agctagcgaa atcaagttga
     4861 tgacttcatg agaatcttcc gcatagatac ttgcccctag aatcaagtca gtttcagggt
     4921 cgactaaaac tttgaataca cctttaggat cttctaatac ttgtgcttta gggacaccgc
     4981 ttgtcatata cttgaataat ttgtaattga cgccttgtgc tttggcttgt ttttctgtta
     5041 aacctacatt ggataaagtc ggagtgataa atacactgta aggcacagct ttccggttat
     5101 tcgttgttcg tttattctcg cccttcaact gatccataat gattcgataa tcatctagag
     5161 agatataagt gaactgcaat ccgcctttga cgtctccaat cgcccaaatg ttgtctgcat
     5221 ctgtacgaag gtattcatcc acagcgattg ctccgcgatc tgttagttta acatctgtat
     5281 tttctaatcc aagtgatgct gtacttggtt ttcttccagt tgcagccaaa acttttgacg
     5341 cttggattgt tacttcttgg ccatcttctg taaaggtgac ggcagctgtt gattcttggt
     5401 ctgctacttg atctacagat acacctagat ggaattcgat tcccgcattt gtcatgtctt
     5461 ctaaaatcat ttgcgcgata tcttcatctt cacgtggaag aaattcagaa tgggcatcaa
     5521 gaacgacaac tttagatccg tattcattga acattgatgc aaattccaag ccaatatatc
     5581 cagccccgat gatcactaat ttttccggta attgttcctg atccatcgct tgcgtagaat
     5641 caatcaagta gcgacttgta ttcaatcctt tgattggtaa aatgattggt tcagaaccag
     5701 tattgatgaa aatccgttcg ccttcaacaa tcactttttc accattattc gtcacttcga
     5761 tttgatggtt agaaagaaaa cgtgctgtac cgtccatcac tgtcccagtt gcctcatcgc
     5821 tgatcatatg ataatttttg gcgcgaagca tgcccgtcaa tttttctttc cgagcaacag
     5881 cctctgtaaa gtcgattcct ctttctccat tgaaaatcaa tgatttagaa ggaatacagc
     5941 cgatgttgat acatgttccg ccatacatac gtgttgactt ttcaacgact aaaaccgatt
     6001 caccttttga agctaacgcc ttcgctaaag tcttaccgcc ctttccaaaa ccgatcacaa
     6061 tattttgata tttttccatc atttttccct cgcttctttt ttacctaccg attggtacaa
     6121 aaatatgata accgataatt gtctattctc caaatcatct gctttatttg tcaaaaaaaa
     6181 ccagacaaaa agcggtcctt tcacgttttt agtctggttc gatttccttt attcttctaa
     6241 acaatcgtta cttctacatc gatatttcct ctagttgctt tggagtaagg gcagaattca
     6301 tgcgcatctt ctgcaatttt ttgggcttct tcttcgctga tgccatcgat atgcacttct
     6361 aatactacac cgatctgata gccgccatca tctaaattat aaagagagac acgtgctttt
     6421 actgtggact ctgctgtcat tcctgcttct ttgatcatat gttgtaccgc cccattgaaa
     6481 caagaagcat aacctgctgc aaataattgt tctggatttg ttccttctgc atggattcct
     6541 ggaggagtga ttttcatatg catcgagcca tttggtgcct cgacttctcc attgcgcccg
     6601 ccatggttga tcattgctgt ttcgtatgct tttttcattg tactgctcct ttcgattgac
     6661 catttgctac ttggctataa ttatagtgta tgacaagtaa ggcgctttct acaaaaattc
     6721 tgcttattcg aataccagaa tgccgtcttc tactattttt ccaaaaaaca aaaaaatact
     6781 actcgtgaac aacagcgtca tatttgttcc aaaataaata gcaatctttt ctacataatc
     6841 ttttatatga ttgattttaa gatacatata taccagaaag ttgaccccat aaaccaatac
     6901 tgttaaaaaa ataaccatat tcgttaccat tttttcctcc ttttgctggt ctccctacca
     6961 gcactcgatt taaccatacc actttttttg ccgtccaagc aactagcctt tcaaaaagac
     7021 tggtttattc tgctgttaat gattcaagat cagaaaaatg tccgaataat tttgctaatt
     7081 ttactaacgc caacttgttc tttccgaatt catcattttc tatctgtaat actagtaacg
     7141 gctcatgtaa gcttaaacgg atcaaaaacc agccgtttcc gtatggctct tctagatcat
     7201 atcgaatacc ttcttcattt tccgggtcga agttcatccc tttttcttct tccaacagtc
     7261 gcttcatttc ttctaatatc ttgtttcctt gttcgcgtgg ttcatcgctg aacaatttta
     7321 atcggatttc ttgtacttct acgggctgtt tcaactcttt gatttgttca tctaatgtga
     7381 taccttttct ttttaattct ggcaaaagca tcaggatctt tgcgatgaca tatgcaccat
     7441 catccagaaa atagttttcc ttgaatgcag cgtgtccgct tgtttcgatg gctagctgtg
     7501 tatctttccc aagacggtta agttctaatg ctttattgat tacatttcga tagccggaaa
     7561 tataacgaac ttggatgcct cctttttctt caatgaatct ttgtagatga gaagaagtag
     7621 gggaattcgt cacgattctt gttcctggat gctcttttaa tacgattgta cttaatactg
     7681 cgatcaaatt atttcgattg atcactgttc cagaagcact gacaactgca gaacgatcca
     7741 catctgtatc gaaaatgatc ccaagatctg cttcatttgc taaaacagcc tgcttgatac
     7801 tttccatcgc ttctttatta tcaggatttg ggatatgatt aggaaatcgg ccatctggtt
     7861 ccaaaaattg ggaacctgtt gaatccgcac ctaagctatt caatactttt tcagcaaaga
     7921 atccaccagc accatttccg gcatcaacaa tgattttcca accatttagt ggtttgcttt
     7981 ctagtgttcc aatccccttt ttaatttttg tgactaaatc ttgcgcataa ggtgtcaaaa
     8041 ggtctgcttt ttctacgctt ccagtttgtt cgctttttat tttttgctgc tgtgatagga
     8101 taaaagcaat atcttctttt tctgctcctc cttctgaggt aaagaatttt attccattat
     8161 aataataggg aagatggcta gcagtcagca taaccgcagc atcgcacgag aactgttcaa
     8221 attgagtgga cataaacata gcaggcgtag tagctaattc gaaatcgatg acatgtatcc
     8281 cttgttcttt cagactttgg atcaacgcct ctttcagtgc tggtccgctt atcctactgt
     8341 catgaccaat cccaatcgtc cattttttat ttggatcggt tgatttcttc tttttcttta
     8401 tccagtttgc tagtccccag ccgatctttt ttaccatgtc tgtcgtcaag tttacttcat
     8461 atccttcaac tggtaatgcg attcctcgaa tatccgatcc attttgcagc gctttcatat
     8521 ctttcatata aaagtcctcc tcatttattc cctcattttt tccagctttt ctgcaaatcg
     8581 ctgtgcttct tcttctgagc ggcaaataat aatgaatgta tctacaccgg ccatcgttcc
     8641 gacaacttca gggtaagcca cttcatctag ccaattcgtt accacatcgg caccatttcg
     8701 ttcagtcaat acgataacca tgaactgaac agactggata cgcaaaacct cgcgtctaat
     8761 tgctgtacgc aaacgttctt catttaaaga aacagatggc tggttaaata atgtatagcg
     8821 tactattttg ttttcatttt gactttttac taacttcatt tctcgaatgt ctcgagaaat
     8881 cgttgcttga gtcacatcgg cgcctgcttc atgcagccgc gcaagcagct cttcctgtgt
     8941 cccaataggt tgttctgtaa tgatctggcg gatcagtgct tgtcgctgtt tctttttcat
     9001 attagacacc tctcaatgtt gattatagca aaagataagt aatatttctt catattttgg
     9061 tattttttac ttttattttt tgttttcatt caggttgcta acctttttct attttaacac
     9121 gcaaaatcgt cttcattttt tcaggtgctg attttcttgg atcgctgatg taaatctctt
     9181 tgtgttcttt agaagctctt tcatagcctt cttttgcgat gaatgcttct aatttctcaa
     9241 acgttgctgg ttcttcggca tagggcccga cgtgtaagat ttgggcacaa agaccttctt
     9301 ccatcttttt gaatactact tgttctttca agtcttccgg aagtttgcta gaacgctgaa
     9361 tggcttcttg tgctatcgct tctgtaacaa agtcaggctg tcggatcatc aattgataag
     9421 aaaaatgttc ttttttcatc actggttcat gcaaatactt ttcttgcagt ccccattgtc
     9481 cttccagtgg atagacagta tacatctgat aatcaggaat cagccaattt tttttaggcg
     9541 acatgcgtat tgcatagctg actgcatata gacaggctac tcttcgttta aagtcttcct
     9601 gattcgggtc gccgatccct ctgatgctaa agaatgattt tttaggaacc tctagtattt
     9661 gcgggctttt tttcatacta tagctcttgt ctttacgcca ttcaattttt tccatttttc
     9721 ttcctccgcg aaattccatt tagttattta ttatcatacc tctttttgag tgacttggct
     9781 aatcgtgttg acactatttt tcagtttgtg ctagaataag tgaagtaaat aaatcgtaca
     9841 gataacacgt ttcggcgtgt cgagagagag gcggtttcta tttttataat agaaatcact
     9901 ctcttttttt acgaaatttt ttagaggtga tgtaaatgac aaaacaaaaa cagctgcttt
     9961 tagtacctgc agtaattgca acagggatga tgtcttttgc gggcgtattg atcgagacgg
    10021 ccatgaatgt aactttccca actcttatca gacagtttga tttaacgact gcacaagtcc
    10081 aatgggtaac aacgatttat cttttgatga tttcgatcat tgttccgctg tctacttatt
    10141 tgaatcgcaa ttttagtctc aaaaaactct ttttagcttc aaatctgctt ttcttggcag
    10201 gtataatgat cgatttcttc tctcctacct tcatggtcct attgtttggt cgattgctcc
    10261 aaggtgcagc gacaggtatc gctcttccgt tgatgttcca tattatcttg acatttgccc
    10321 ccctcaatcg acgaggcgcg atgatgggtg tcggtacttt gacaacggcc attgctccag
    10381 caattggccc cacttatgga ggcattatga cttcacattt tacatggaat catatcttcc
    10441 tgtttcttgt gcccgttttg attctttctt tgattatcgg ctggtttgct attcccgaga
    10501 tggcagtaca aaaaagtggt tatttggatt ggataagcgt tttaggaatt tgtcttttgt
    10561 ttagtggaag tctaacattt ttgagtacgt tgggaacagt ttccagctgg attgctttaa
    10621 tcatcggcgg tattggctgg ttcctttttt actatcgaag taaaaaagtg gagcatccac
    10681 tgatccgact gtctattctt tcaaataaaa cattccgctt gtttttgatt ggcttcttag
    10741 tttatcagtt tttattactt ggtgtatctt ttgtcttgcc gaatttcctt cagatcgttc
    10801 aaggaagtag tccttttgtc gctggattag cgatgcttcc cggcgcagcg gttggtgctt
    10861 tgctttctcc tttttccggg aaaatgctgg atcaatatgg tccgaagaag ccaatttttg
    10921 ctgggctgat tctttctttg attggttggc tagcattatt cctactcata ggacacgctt
    10981 cagtgggtgt attgattgct tgtcatgtgt tttatatgat cggtatcgga ttatcttaca
    11041 gcaatatgat gaccaccgga atgaatgcac tgaatgaaga actacaagga gacgggaacg
    11101 caatgttcaa taccttgcag cagttttctg gcgctgtggc tacttcatta gtagcggtca
    11161 ttatcaatta cgtccagcat catacttccc ataactatga agtgtctact acattaggat
    11221 caaaagcagc attaggcgtc ttgttgctgt tgcttctcgt aagctttgct cgattcgcct
    11281 attatctgtt ctttgctaaa aaagcgtaga ttccatatac ggcaggtgtt gcatctgccg
    11341 tttttttgtg tgaaaatggt tgtttgtaaa aggaggcttt tttatggaaa aaattgccat
    11401 tatcggtggc gggatcattg gtatgaccct agccagttac ttagatacat caaaattcaa
    11461 tgttgcttta tttgatgaag ggacaggaca ggcaactaaa gctagcgctg ggattatctc
    11521 cccttggttg tcgaaacgca gaaacaaaca atggtatcgt ctggctaaag acggcgctgc
    11581 ttttttccct aaacttataa aagatttcca acttgatcaa acgatttacg cacaatcagg
    11641 gacacttttg cttcgacctg aaaaagattt agctgaattg gctgttcttg ccgagaaacg
    11701 aaaagaagaa gcgccagaaa ttggaaacat cgaatggctt tcacctgaac agacatctca
    11761 agcacttccg ttattgaaac cagcagtttc actgcgtatc agtggtggtg gtcgtctgga
    11821 tggaaaagca tatttagaac atcttggtca gcgtttagca caaaaacaaa tggagcagat
    11881 ccaggcaaaa gtcactttgc ataagaagag cgattcttgg ctcatcaagg gaccgtttgg
    11941 agaatacaca gctgatcgtg taattttgac acctggcccg gcacttaagc ctttattaca
    12001 agaattaggc tatgctgcag atatacgtcc gcaaaaaggg cagctcttag catttcagac
    12061 atcttataag atgagtcaag actggcctgt tgctatgctt gacggagaag ctgatttcat
    12121 tcctttcatg gatggaaaga tccttttagg tgctacccat gaaaacgatc aaggctggga
    12181 tttggctcct actagcgaag cttataaggg attgactaag agtacagaac agttcttgac
    12241 tgatccagat cagataaaaa actggccata cgagttccgt gtaggtaccc gtgcttatac
    12301 gtcagatttt tccccatttt tcggcgtact gccagatgat ccaagcttac tggctgctag
    12361 cggtctaggc tcttctggct tgaccacagg tccttatatc ggctatctat tagccaactt
    12421 cttaaatact ggtcattggc ctacggaaga ttatcaaaag gacatctcca cctatattac
    12481 gaaaaatcca tcatagaaaa acagcttttt caagtaagga tcccgagcga tattgcgcat
    12541 ggattacttc ttgaaaaagc tgttatttta gttcaatttc tgaatcaaat tctccaaact
    12601 ttcttttgct tcatctggca agagatcttt tcctatctga gcttcaatga atcgtaaacg
    12661 aatatcaagt cgttgtttca ccagattttt ataggacgta tcctcaaaag aaacaggaaa
    12721 atctggtata ggtaaatagt ggatgtcttc aaatggacca aacgacttga attctgccgt
    12781 ttgttgcacg attctttcaa cgattgacaa tgtcgtttca ggtttgattt ttttatcatc
    12841 gaaatatcca gtattcagga catagcaggc tgtttgctgt ttcaaaaaca actctcgaaa
    12901 agcttgatag tcttccctaa gaggatagac acggaaagga ttagcaaatg gttcaatgac
    12961 tagctcatct agctgtcttt ttgtagtgat attttccgcg tctgatcgtt ttgttgctaa
    13021 tgttgcacca aaagacgctg ctaaaactgg gtcattgatc ttgataattg gcggaagcga
    13081 atcatctttc atgatccaaa aaacagcatc gatcttttcg ctaagatgat cgactcggtc
    13141 gggtgtcgca taacgtgatt tgactgtccg cccatttccg tttcgtatat ctcctgttac
    13201 taggactttt tctcctttac ttgtacatgt cacgcccaca ttctgacaag taaggaaaga
    13261 agaaatggcc ggatcatcag acggatagtc ttgtgtttta tcaaagtaag aaggttccaa
    13321 ggcagtagca aaaccatttt ttcgagaaat aacgaaagca tcgtcatgca agatcgaaac
    13381 agagtgacct tccgtatgtt ttgctaaggt aatcgttgat tttccagaac cggacaaccc
    13441 aaatacagcc atcgtatagg tctggtcatt actagcatat tgtttcatcc caccgtggca
    13501 ggcaataaag ccattgcgat ttcccaatgc ccaagcaagc gtcaaagtcg ctttttttaa
    13561 ttctccaaag taacgtaatc ctagcacaac cgctgtatta tggagagcat caaacaaaat
    13621 gagtccattt ggaaatgttt catgtgtcca ttcgggatca caaaagatat aaagatctcc
    13681 ctcatcaaag gaaacagact tttgataagt ctctaagatc ttcgaattcg ctggctgaaa
    13741 attcagcaaa taagaataca aattgttctc aaatccttct ggaatcagca aatgactttg
    13801 cacagaaaat tcttcagata ggccaacgat tacttttgta tggtaaaact ggcgcttgcg
    13861 actttgatac acagcttctc ttaaaatttt ctcgtatttg ctttgttcct tgtctaagtc
    13921 ctcaaagatc cgccgagcac ttgctgttct tcccacaatg cttccatcat tgaatactag
    13981 tgtcctaaca tcagctggta aatctagttc ttcagcatgt gagattgtta aatctgtgat
    14041 gatcgttcct ggactccctt ttgctagttg ataagctgtc ttcctatcaa aaacttcatg
    14101 aacattatta ccgtaaaatg tcgtttctac aatcgatcga atactggata acagtggatt
    14161 tccacttgtg atttcgttta attcgagact ctcttttgta ctcatggctc ctcctcattc
    14221 cctaaacaaa aaaacgtttt ctttattata cattattcga gtttttattt gtactgctgc
    14281 gctgtgggaa gcaaacgagt tatttttcac acaaaaaaac cgagaacttg aataaacaag
    14341 tttctcggtt tcatcatttt ccttttatcg gcttagtacc agccgtttgc taaccagaat
    14401 gttttagcag catcccatga accataacga gatgttacat attgttctgc tacacgttct
    14461 tgattggcag ctgaatagtc gccgtttaag tatgaagcat ctaattgata acggccgatg
    14521 taacgtccgt tagtagctgt gtatgaaccg cctgattctt tttgtgcgat ccattctttt
    14581 gcagagcttg aagttggcgc tgttgcagca gctggggcag caggagctac ttcaactgtt
    14641 tcaacaggtg caacgtatgt atgttcttgc gttgctgttt cagttgtaga tgctgtattt
    14701 tctgttgttt tgttttctgt tgttgctgaa gaatcatttg aagttgggat tgttaattgt
    14761 tcaccaacat aaatgcggtt gatgtttgcg atcgaatttt tttcagcaat tgcatcgatc
    14821 attgagttgt ctcctgcaaa tttttgtgag atcttagata atgaatcacc tgattttact
    14881 gtataaactt catcagcgtg agcatttgta gttcctacaa acattgcagt agcaccagca
    14941 gcaaaagttg tacctaaaac aattttctta aatgaattca tattaaactc ctttttttct
    15001 ccgtttttta ttctttcgac gctattaact ttatcattta g
//
LOCUS       DAXWLF010000057        14922 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXWLF010000057 DAXWLF010000000
VERSION     DAXWLF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.057"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(21..251)
                     /locus_tag="ACGE4U_001343"
     CDS             complement(21..251)
                     /locus_tag="ACGE4U_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393517.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="ACGE4U_001344"
     CDS             complement(687..1253)
                     /locus_tag="ACGE4U_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8393518.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="ACGE4U_001345"
     CDS             complement(1253..2209)
                     /locus_tag="ACGE4U_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFF8393519.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="ACGE4U_001346"
     CDS             complement(2209..2865)
                     /locus_tag="ACGE4U_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFF8393520.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="ACGE4U_001347"
     CDS             3235..5253
                     /locus_tag="ACGE4U_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8393521.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /gene="leuS"
                     /locus_tag="ACGE4U_001348"
     CDS             5680..8094
                     /gene="leuS"
                     /locus_tag="ACGE4U_001348"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFF8393522.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="ACGE4U_001349"
     CDS             8442..9995
                     /locus_tag="ACGE4U_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFF8393523.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="ACGE4U_001350"
     CDS             complement(10376..12160)
                     /locus_tag="ACGE4U_001350"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393524.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="ACGE4U_001351"
     CDS             complement(12175..13896)
                     /locus_tag="ACGE4U_001351"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393525.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="ACGE4U_001352"
     CDS             13996..14757
                     /locus_tag="ACGE4U_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8393526.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4836 a         2757 c         2942 g         4387 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt ct
//
LOCUS       DAXWLF010000058        14820 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXWLF010000058 DAXWLF010000000
VERSION     DAXWLF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.058"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(296..1024)
                     /locus_tag="ACGE4U_001353"
     CDS             complement(296..1024)
                     /locus_tag="ACGE4U_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8393527.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACGE4U_001354"
     CDS             complement(1002..2576)
                     /locus_tag="ACGE4U_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFF8393528.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACGE4U_001355"
     CDS             2805..3743
                     /locus_tag="ACGE4U_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8393529.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACGE4U_001356"
     CDS             3755..4666
                     /locus_tag="ACGE4U_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8393530.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACGE4U_001357"
     CDS             4690..6162
                     /locus_tag="ACGE4U_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8393531.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACGE4U_001358"
     CDS             6213..6824
                     /locus_tag="ACGE4U_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFF8393532.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACGE4U_001359"
     CDS             6854..8767
                     /locus_tag="ACGE4U_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFF8393533.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACGE4U_001360"
     CDS             8895..11876
                     /locus_tag="ACGE4U_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFF8393534.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACGE4U_001361"
     CDS             11873..13534
                     /locus_tag="ACGE4U_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFF8393535.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACGE4U_001362"
     CDS             13574..14506
                     /locus_tag="ACGE4U_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFF8393536.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4818 a         2332 c         2979 g         4691 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat atcaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
//
LOCUS       DAXWLF010000059        14525 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXWLF010000059 DAXWLF010000000
VERSION     DAXWLF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.059"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(41..1006)
                     /gene="nrdF"
                     /locus_tag="ACGE4U_001363"
     CDS             complement(41..1006)
                     /gene="nrdF"
                     /locus_tag="ACGE4U_001363"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFF8393537.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(1056..3215)
                     /gene="nrdE"
                     /locus_tag="ACGE4U_001364"
     CDS             complement(1056..3215)
                     /gene="nrdE"
                     /locus_tag="ACGE4U_001364"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFF8393538.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            complement(3374..3598)
                     /gene="nrdH"
                     /locus_tag="ACGE4U_001365"
     CDS             complement(3374..3598)
                     /gene="nrdH"
                     /locus_tag="ACGE4U_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFF8393539.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            3999..4472
                     /locus_tag="ACGE4U_001366"
     CDS             3999..4472
                     /locus_tag="ACGE4U_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFF8393540.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            4469..6601
                     /gene="feoB"
                     /locus_tag="ACGE4U_001367"
     CDS             4469..6601
                     /gene="feoB"
                     /locus_tag="ACGE4U_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFF8393541.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            6603..6737
                     /locus_tag="ACGE4U_001368"
     CDS             6603..6737
                     /locus_tag="ACGE4U_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFF8393542.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            6831..7475
                     /locus_tag="ACGE4U_001369"
     CDS             6831..7475
                     /locus_tag="ACGE4U_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8393543.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            7662..8855
                     /locus_tag="ACGE4U_001370"
     CDS             7662..8855
                     /locus_tag="ACGE4U_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8393544.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            8848..10575
                     /locus_tag="ACGE4U_001371"
     CDS             8848..10575
                     /locus_tag="ACGE4U_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFF8393545.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            10909..12081
                     /locus_tag="ACGE4U_001372"
     CDS             10909..12081
                     /locus_tag="ACGE4U_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFF8393546.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            12332..14194
                     /locus_tag="ACGE4U_001373"
     CDS             12332..14194
                     /locus_tag="ACGE4U_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8393547.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            14194..>14525
                     /locus_tag="ACGE4U_001374"
     CDS             14194..>14525
                     /locus_tag="ACGE4U_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8393548.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT         4672 a         2537 c         2998 g         4318 t
ORIGIN      
        1 ctttttattt ttcgaaaaat ttttgaatta aaataaatcc ttattccaat ccaatcaaat
       61 aatcgtcgtc tttcatcgct tctacatgac ctaacaaata gccgtttccg acttgtgaga
      121 agaagtcatg gttgcttgtg cctgttgaaa tcccgttcat tacgattggg ttgacatcat
      181 tggcagtatc tggaaagagt ggatccatcc ctaggttcat caatgctttg ttggcattat
      241 agcgtaggaa tgtcttgact tcttctgtcc agccgattgg atcgtataat tcttctgtat
      301 aacgttcttc gttttcgtaa agctcataaa gaagattgta catccagtct tttaacgttt
      361 cttgttctgc ttcaggtaac tcgttgaatc ctaattggaa tttgtaaccg atgtaggttc
      421 catgtacaga ttcatctcgg atgattagtt tgatgatctc tgctacgtta gccaatttgt
      481 tgtttcctaa ataataaaga ggagtataaa aacctgaata gaacaagaat gtttctaaaa
      541 agacgctggc aattttcttt tctagcggtg taccgtgttt gtagatttca ttgatacgct
      601 cagctttttt ttgcagataa ggattcgtat ttgtccagtc aaaaatttct tcgatttctt
      661 ttttcgtatt cagtgtacta aaaatagaag aataactttt ggcatgaacg gattccatga
      721 attgaatatt gttcaaaacg gcttcttcat ggggagtgcg gacatcttta cgcaactgat
      781 ccatgccact ttcagattgg acagtatcta gcagtgtcaa accgccaaat acatgtccta
      841 ctgtatcttt ttccaattga gacaatgtac gccaatcatc taggtcattt gaaagtggga
      901 tacgtgtatc tagccaaaat tgctctgtca gcttctccca agtggattta tcaatgatat
      961 cttcaatttc attccagttt attgcttcat aatatgtttc tgacatgaga atgctccttt
     1021 aattaaaata tgaataagcg aatctatcta tggtattaga tcacgcagct ttcacattga
     1081 ttgcttccga tttcttctgc atcatcagta aaggtacgta cgtaataaat ggatttgatt
     1141 cctttgtgga aagcgtaatg tcttaaaata ttcaaatcac gtgtcgtttg cttcgttgtt
     1201 gtcttccatt cataaatgcc ttctgggatt tcagaacgca tgaataaagt caaactcatg
     1261 ccttgatcga cgtgttgttg tgccgttgca taaacatcga ttactttacg catgtccata
     1321 tcataagcag aagtgtaata cggaatcgtc tcattggata gataaggcgc tgggtaataa
     1381 attttaccga ttttcttctc ttggcgttct tcgatcatgc gagtgatagg atgaatactt
     1441 gcactcgtat cgttgatata tgaaattgag ccatttggtg ctactgccaa gcgattttga
     1501 tgatacagtc catctttcat tactgcttgt ttcaaagcat tccaatcttc agcagttggg
     1561 ataaaaatgc cttcaaacaa tgctttgact ttttctgatt ttggctggaa ttctccagtt
     1621 acgtattttt cgaaataagt gccgtcagca taagctgatt tttcaaaatt atggaaaact
     1681 tgttttcttt cacgagcgat attgttgctt tcaacaagtg tccaataatt caacagcatg
     1741 aagtaaatat ttgtaaagtc taatgaatcc tctgagccat attccatttg ttcttttgca
     1801 agataagtgt gtaagcccat ggcacctaat ccaatcgtat gattcagctt atttccgttt
     1861 tggatcggtg gtacgacatc gatatcagat gcatctgtaa taaatgtcaa tgcacgagtc
     1921 atcgtacgca cagattgacc aaagttaggg ctttgcatca aattcacgat atttgtcgag
     1981 cctaagttac atgagatatc tgttcctaat acttcatatt cctgtttgcc gttaatcaca
     2041 gaaggcgttt gaacttgtaa aatctctgaa cataaattac tcatgatgat cttgccatcg
     2101 attggattct cgcggttcgc tgtatcaata ttgattacgt aaggatagcc agattcttgt
     2161 tgtaattttg agatttcatt ttcaagatca cgcgctttga ttttttgttt gcgaatgttt
     2221 ggatttgcaa ctagatgatc atactcagct gtaatattta cataagagaa tgggacacca
     2281 tattcttttt cgacactata tggactaaat aaatacatat cttcgttttt acgtgtcaat
     2341 tcataaaatt tatcaggtac gatcacgcca agagaaagag tttttacgcg aatcttttca
     2401 tctgcatttt ctttttttgc tgaaaggaaa gcaataatgt ctggatggaa aacgttcaaa
     2461 taaaccacgc cagctccttg acgctgacct aattgattag aatagctgaa actatcttcg
     2521 aataatttca tgactggaac gacaccactt gcagcaccct catatccttt gataggagcg
     2581 ccggcttcac gaagatttga taaagtaatc cctacgccgc caccaatacg ggacaattgc
     2641 aaagcagagt tgattgagcg cccgatagag ttcatatcgt cagtgacttg agtcaagaaa
     2701 caagacacca attcgccccg acgtttacgt ccagcattca agaaagaagg tgtagctggc
     2761 tgatatcgtt gatggatcat ttcgtctgca agagcaagtg ctaattcttc atccccgttc
     2821 gcaaagtaaa gcgcattgaa ggctactcga tcttcataag tttccagata agcggaaccg
     2881 tcgttggttt tcaacgcata ttgagaataa aatttgtaag cagccataaa agatttgaaa
     2941 gaaaaatgtt gctcgtccaa aaattgatat agcttttcaa taaatgatgg tgaatacaat
     3001 tgaatgaatt ctgcttctat atattcttga tcgatcaagt aattgatttt ttccataata
     3061 gatggaaaag tcatcgtatt tggtttgaca ttttcctcaa aaaaggcaac taaagcttct
     3121 ttatccttat tcaaagggat ttgaccattg actggccgat tgatttcatt attcagcttg
     3181 aaataactga catcttgtaa atttttaaga ctcatacgta cgcccaacct tcatttcgta
     3241 tttgatgatg cttgattgca aatcattgca ttgtttttaa gctttaaaaa aggatttgga
     3301 agtttgcgac aaaaactggt gctgtctcaa gcccttcaaa tccatgtatc acgttttgat
     3361 tcttttagtc atgttagctg gcaagtttac gcaactgatc aggacggaat ccgataactg
     3421 tttctgcatc agaagtaatg acaggaacac tttggaatcc ttgtgctttc aaccagtcta
     3481 ctgcttcagg ctgttcatcg atattgactt cttcaaaagc aatttgattt tctgttaaaa
     3541 accgtttgac catcttacat tgcatacaat tatttttaga aaagattttt acgttcatgg
     3601 tgagtgcctc ctctaacaca ttcttattat ctataataga tagtataaaa ctcttagtaa
     3661 aaaagtcaaa caacaaacac tacatattgt gttgtggaaa ctgtggaata ctaagttttg
     3721 tgtttttgaa aaaagagctg aaaacaagta tcgtgtggga aaatagcaaa tttaatagag
     3781 aaaaaattgt ggaaaactat gaagaaatat ttttttctca tttttatcta tcaatactaa
     3841 agattcgctc ataatatcac aagtataatt gacattctca attaccgaga gtattctatg
     3901 tgagaatgat tctcatttat ttgcgtaaca cgtaagaaaa ttagaacata taatgaatga
     3961 ttttaataca tgatttattt cacaagagag gaatcaatat gcagacctta gcgcaggcaa
     4021 agcagaattt cgtttatcaa gtcgaacgaa tcgaaacaga agaaaaaaca aaacgccatt
     4081 tgcaaaatat cgggatcgtt gccggaagta aaatcgtcgt cgtcaattta tcaggcaaca
     4141 atgccatttt actggtaaaa agtcatcgag tggcagtaag tcgggaaata ctaggtcaaa
     4201 tctttgtcaa taaaaagact gcttcctctg aaagctggac ttctcttgat caattaaaag
     4261 caggagagac tgggatcgtc gtagcaatcc acggacaagg cgcagtgaag cgacggttaa
     4321 tggatatggg attgacgcga aatgtaaaaa tagttatccg aaaactagcc cctctgggag
     4381 atccaatcga actgacagtg cgagggtatg aactgacgtt acgaaagtca gaggccgaac
     4441 taatcttgat acaaagggag gaaaaagaat gaatatcgcg ttagtcggaa atccaaacag
     4501 cgggaaaact agcttgttca atctcttgac tggttcgaat caatatgtag ggaattggcc
     4561 gggcgtaaca gtagaaaaaa aagaaggatc gttgaaaaaa aatccgaaca ttcatattca
     4621 ggacctaccc ggcatctatt ctttgtctcc ttatacacca gaagaagtga ttgcaagaga
     4681 ttatttggtt caggaacagc ctgaactgat catcaacatt attgatgcta ccaatttaga
     4741 acgaaatctt tatttaacta cgcagttatt ggatttagac attcctatga tcattggtct
     4801 gaatatgatg gatgtcatca aaaaagaagg aaaaaaaatc aatctagaaa agctttccta
     4861 tggtcttggt gtaccagttt taccaatcag cgtattgaaa aagcaaggaa tcgaaaaact
     4921 aatcgaaaag attcaggagt ctgcaaaaaa gcggccttct attcctgttc cctgtacata
     4981 tgatccacgt ttagaagcag ctttacatga aattgaagat gtactaggag aacaaagcgg
     5041 caagaaacgc tggtatgcaa tgaaatattt tgaacgagat cagaaggtaa gagaggatta
     5101 cgagatagca gtttctcagc aaaaagagat cgagcaactg atccagttaa cagagaaact
     5161 attggacgac gatagtgaga cgattctggt caatgaacgt tatgaattta ttacccaact
     5221 atgtacactc tgtgttgtgt caaatgattc tttccaatta tcgatgagtg acaaaatcga
     5281 tcagattgca acgaatcgtt ggttagcctt gccgatcttc gcatttgtga tgtggctgat
     5341 ctattatctg gctattcaaa cagtaggaac aatggggaca gattggatca atgacacatt
     5401 gtttggtaca tggttaccag aacatgttgg cagattgtta gctgaatggc aagtagcggg
     5461 atggatgcag gagctgatct tgaacggcat catcgcaggc gtgggagcag tgttaggatt
     5521 tctaccgcaa ttgattatat tgtttttatg cttgagcttt ctggaagact gcggatatat
     5581 ggcgaggatc gcttttgtca tggatcgact tttcaggaaa ttcggtcttt caggaaaatc
     5641 cttcattcct atgctgattg ctacaggatg tggagtgcca ggcgtcatgg ccagtcgcac
     5701 gattgaaaac gagcaagatc gtcgtttgac gatcatggtc acaaccttca tgccttgttc
     5761 tgcaaagttg ccgattattg ccttagtagc aggggccttt tttcctcatc aatcttgggt
     5821 agctccttct gcctattttc ttggtatgac tgcaattatt ttctctggaa tcagcttgaa
     5881 aaaaacacgt ttgtttgctg gagatacagc tccttttatc atggaattgc ccgcatatca
     5941 ttttcctcaa tggtcagcgg tcctaagaca aacctatgac cgcagcaagt cttttgttaa
     6001 aaaagcagga acgattatct ttgtttctag tatcgtgatc tggttcagct cttcttataa
     6061 tttttatgct caacctgttt ctgaagacca aagtatctta gcctttttcg ggcgaatcct
     6121 ggcagttgtt tttcagccgc ttggttgggg aaactggcaa ggaacagtcg cagcgattac
     6181 aggattggtc gctaaagaaa atattattgg cacatttggt attttgtttg gtcatgcgga
     6241 agtatccgaa aatggcagag aaatctggca agtgctacaa cacacatata ctcccgcagc
     6301 agcttattca ttgttagcct tcaatttact ttgtgcgcct tgttttgcgg caattggggc
     6361 tatccatcgg gaaatgaatg ccaagaaatg gacttggatc gccataggct atcaatgcgg
     6421 tttagcttat ttagttagtt ttgtgattta tcagttggga cacttattgg agggtggaca
     6481 ggtagcgtta gggacgtatc ttgctatttt actgatgata ggactcgttt atgttttgtt
     6541 ccgtcagcca aaatccaaga accagtttta tactatattg tcattagaag gagaggaata
     6601 ggatggcgac attcttatta agtgtgttaa tctttggaag tgcaggcgca attgtctata
     6661 ctcgtttaaa atccggtaag aattgtgatg actgtcaaac agcttgccca gtaaaaaagg
     6721 aacagatcaa gcgatgatca gtcgttgcaa gaggctattc tccccttttt tgcttatttt
     6781 gctagagcat agtaagatga gggaaagtaa aaaaagaaag aggaaagaaa atgtctggaa
     6841 agaaaaaaat cgtccgcccg cgctaccagc agattgctgt tgatttagcc gaacgtatcg
     6901 ttgaaaaccg atataaagtg ggtgagaaga tccatgcccg ctcaacactt gccagtacgt
     6961 acaatgcttc tcctgagact acccgcaagg ctatcaatgt gctggtagat cttggaatca
     7021 tggaagtacg gcatggaagt ggggcatttg ttgcttcgaa ggaaaaagca aaagatttcc
     7081 tattgcaata tcaagatgta caatccatcc aagatacaaa agcagaaatc atggcaagtg
     7141 tggagaagca acaagatgag ctgaatcatt tttctcaatt attggatcag ctggtcaatc
     7201 aaacgaagcg aatacataag atgaatcctt tacttccgtt tgaattatct ttatcagaaa
     7261 aagcaatgca tctggaccgt tcaattagcg agttgaatat ctggcaggcg actggcgcca
     7321 cagttatcgc aatttctcat aatgaagaac tgcttgtttc acctggcccc tatgctaaat
     7381 ttacggcagg ggacatcgtt cattttattg gaaatgaata tacactacag cgtatgacaa
     7441 acttttttta cccggagcat cagcttgcag aatgattgtg tgaaatatta aaaaaatatt
     7501 aacttggcta aaccttttat ttacaaagat ttagccaagt ttttttcagt ttggacaagc
     7561 aaatttcttt catttttgac aaagtgacta catgatgtta acatagtgtg gtcacttttt
     7621 tcagaaagaa aaatgaattt acataaaaaa ggagaatttg tttggtaaaa gtagaagtaa
     7681 aacatttaac aaagatattt ggtaaaaaga cacaagccgc tttagatatg atgaatgatc
     7741 accagccgaa aacggagatt ttaaagaaaa ccggtgcaac ggttggtgtg tatgacgtga
     7801 attttgacgt aaaagaagga gaaatcttcg tgatcatggg gctttccggg agtgggaaat
     7861 ccacattgat ccgtctattg aatcgtctga ttgagccaac ttcgggcagt atctatattg
     7921 atggagaaga tgtatcgaag ttaagcaaag aagaattgcg agaagtccgt cgtcataaaa
     7981 tcaatatggt tttccaaaac ttcggtctgt ttccacatcg aacgatcttg gaaaatacag
     8041 aatacgggtt agaagtccgt ggtgtaccta aagaagaacg acaagaaaaa gcagaaaaag
     8101 cattagaaaa ttctagtttg ctttcattta aagaccaata tcctagtcag ttatctggtg
     8161 ggatgcaaca acgggttggc ctagccagag ctttagcaaa tgatcctgag atcttgctga
     8221 tggacgaagc gttctcggct cttgatccgc tgatccggcg agaaatgcaa gacgaattac
     8281 tggacctgca agccaatgtt caaaaaacga ttattttcat tacccatgat ttgaatgaag
     8341 ctttacggat tggggacaga atcgccttga tgaaagacgg cgaaatcatg cagatcggaa
     8401 caggtgaaga aatattgacc aatccagcaa atgattatgt acgtgaattt gtggaagaag
     8461 tcgatcgttc caaagtgctt acggctcaaa acatcatggt acctgcattg acgacgaata
     8521 tcgaatctga tggaccaaac gttgcattga caaggatgcg taatgaagaa gtcagcatgc
     8581 tgatggctgt tgacagaaag agacacctga aagggatcat cactgcagat caagcgttag
     8641 aagctaggaa gcaaaaacgt ccattgatcg acttcctaga tgaaaatgtg acggtgattg
     8701 ggaaagacat ggtggtaagt gatattttca atattattta tgactcaccg actccacttg
     8761 cagttgtaga ggatggaaaa ttaaaaggtg tcgtcatccg aggtagtgtg attgaagcat
     8821 tagcagaaac aagcgaggtg agtgaacatg aataatctat tgaactatca attaccagtg
     8881 gcaaactggg tagaaaatat cacagaatgg tttacgacaa ctttttctgg cctattttca
     8941 tttttacaaa cgattggaca ggcagtgatg tcggggatta caaatttact gttagtgatt
     9001 ccagctcctt tgtttatctt gttattgacg gtagcagctt tctttatttc taagaaacgt
     9061 ccaggactta cgttgttcac attaatcggt ctatggttta tttataacca aggtctatgg
     9121 aatgatttga tgaatactgt cacacttgtt cttttatcta gtgtgatctc gatcatcatt
     9181 ggggtaccat tagggatctt gatggcaaaa agcagtaaag cccaaagtat tatcaaaccg
     9241 atccttgact ttatgcagac aatgccagga ttcgtttatt tgattccagc agttgcattt
     9301 ttcggtatcg ggatggttcc aggggtattt gcctcagtca tctttgctct gccaccaacc
     9361 gttcgtttta cgaatttagg gattcgacaa gttcctaaag aattagtaga agcttctgat
     9421 tcattcggga gcacaagtcg tcaaaaacta ttcaaactag aattgccttt agctaaaagt
     9481 acgatcatgg cagggatcaa tcaaacaaca atgctgtcac tttcaatggt cgttattgcc
     9541 tctatgatcg gtgcacccgg cttaggacgc ggcgtattat cagcattgca aagagcacaa
     9601 gtcgggaatg gatttgtcaa cggggttgca ttagttatct tagcaatcat cattgaccgt
     9661 tttacacagc atttgaacca gccaaacaat aaaaaagctg caggagcaac aacacagaaa
     9721 agtaaaaaac gtcaaggact gatcattggt gctgtagtag tagtgatctt aggtgcgatt
     9781 ggtataggga gtttttcatc tgccaaagaa acaaaacgta tcaacttatc ttatgttgaa
     9841 tgggatacag aagttgcttc aacaaatgta gtcggtgaag tattgaaaca aatgggctat
     9901 gatgtaacga tgacgccatt agataattca atcatgtgga aatctgtttc aaatggcgag
     9961 tcagatgcca tggtttcagc ttggctacct aaaactcatg gttcacaata tgcacaatat
    10021 aaagatcaag tcgaagacct aggtgccaat ttgacaggag caaaagttgg tttagcagtg
    10081 ccagcctaca tggatgtaaa ttcgatcgat gagttaaccg atcaggcagg taagaaaatc
    10141 ataggaatcg agccaggagc tggtgtagtt acggctgctg aaaacacgat ccaaaaatat
    10201 gacaacttga aagactggaa agtagagaca tcttcttcag gagcaatgac cgttgcacta
    10261 ggtcaagcga tcaaaaaaca cgaaccaatt gtagttacag gatggacacc tcattggatg
    10321 tttgccaaat atgatctgaa atacttagaa gatcctgaaa acggaatggg aagcgaagag
    10381 cagatccata cgatggttcg aaaaggattg aaagaagacc agccagaagc ttacaaagta
    10441 ttagacaatt tccattggtc agaaaaagat atggaaaaag ttatgttgga aatcaacaat
    10501 ggaaaagatc cacagcaagc agcaaaagac tggatcaaag agaaccaaga attagtcgaa
    10561 agctggaaaa aataaaaaga cagacacgaa ataaatggag cttccaaaat tggatcaaat
    10621 atccaaattt ggaagctcca tgttcgtctc tatctttttt gtctataaaa aaagagaatg
    10681 cgtagctcat cttcgaaacg ttcaaagacg ccgacaaact attatgaatt tatgggtgcg
    10741 agtcaaagag atcgatcgta ggtatgaaag actaatgtga ttaaagttta ttatgtacga
    10801 agtgcattga taaagtccat ataattgtgt aaaagataaa aggccatgta acagcccttt
    10861 tacggtacaa tgtttttaac cacaaaaaca tacccaggag gacgttacat gacccaagta
    10921 cattttacac tgaacaacga agaggttcaa agtattattg aacattcggt aaaagatgat
    10981 gtttctaaaa atattttaac cactgttttc aaccaattga tggaaaatca acgaacagaa
    11041 tatattcaag ccgatgacta tgaacgttca gaaagtcgtc agagtcaaag aaatggctat
    11101 tatgagcgag actttacgac tcgtgtgggt acactcgaat taaaagtgcc tagaacacgt
    11161 gatggtgaat tttcaccgac ggtgtttgag cgttatcagc gaaatgaaaa ggcactgctc
    11221 gcttcaatgc ttgagatgta tgtttcgggc gtttcgacac gtaaagtttc aaagattgtt
    11281 gaagagctat gtggaaaatc tgtatcgaaa tcttttgttt ctagcctgac tgagcagtta
    11341 gacccgatgg tcaacgaatg gcagaaccgt tcactctcag gtacgaatta tccttatctg
    11401 atgactgatg ttctctacat aaaagtccgt gaggaccatc gagtgctttc taaaagctgc
    11461 catattgcga tcgggataac agaaggtggc gaccgtgaaa tcattggctt catgattcaa
    11521 aatgaagaaa gtgatgacac atggtccatc ttctttgaat acttaaaaga acgcggccta
    11581 aaggggacag aactcatcat ttctgatgcc cataaaggcc tagtgtctgc gattcgtaag
    11641 tcatttacca acgcaagttg gcagagatgc caggtccatt ttttaagaaa catcttcagt
    11701 tccattccaa aaaagaattc aaaaccgttt agagaagcag taaaagcgat ctttaagttt
    11761 acggatattg aactcgctcg aacagctaag aatgccttag tcggtgaata tatcgaccag
    11821 cctaaatata caaaagcctg cgaaatattg gataatggct tcgaagatgc ctttcaatac
    11881 acggttatcg gaaatagtca taatcggcta aaaagcacca accttcttga acgactgaac
    11941 caggaagtcc gcagaagaga aaagattatt cggatttttc ccaaccgaac gtctgccaat
    12001 cgattaattg gagctgtcct tatggacctt catgacgaat ggctcagttc tacaagaaaa
    12061 tatattaagt ttgatcaatg agagaccggt aaaacattgt atagtatttt acacaggatt
    12121 atggacttga cttgaaaaag cgtttgatta tttatattac gacttagtgc aataaaaaac
    12181 gctttttaac agtacatgta gtcgtcttta taatagataa actctgtaat attagttaaa
    12241 aagctataag ttgcacttag ttaagtgcaa acatttcata ttgtcgttag tttagaaaac
    12301 gcttatatta gcaatgtaag gagagatgat aatgcagcag ctaacaaaaa gagaaaggca
    12361 gcttttgatt tacttggtgg aaatgtctga accggtaaca atcaaaaagt tggctgaaaa
    12421 agaaaatgtt agtgttcgga cgataaaata cgatctagat gatataagag agtggctgaa
    12481 tgagcggaag caagtcctat atagcaaacg tagtcaggga atttggatga atattagtag
    12541 ctctgatcga atcaaattaa aaagcgaatt gatggatgtt aatcgttttg aaatatttgc
    12601 agatcaaagt ctccgaatca aacgtttact aattatgctt ttagtaacaa aagaagctat
    12661 tactggaatg aaaatggcca acgcattaga tgtcagcaaa gatacgatca tgaatgattt
    12721 agatgtgctg gaaaataatt ttcttaagga aggattgacc ttgaatcgtc aggcgcgaaa
    12781 gggtttttgg atcataggag aagaacgtct agttcgatta acgattgaag agattcttca
    12841 aaaagagttc acggattatg acatttataa attaatgtca ttgttattga atggcggtga
    12901 gactgaatac tttgaaatgt attctgctac tgctacacct atccaagagg tatttaatca
    12961 agtaatcatt cgaatgcgac acttactgga atttgaaaat ctagagaaac tgaattacgc
    13021 ggaattacta aatattctga ttcgagtgac aattgctacc gttcgcttac gcaaagaggc
    13081 tacgattggt cgttatcaat tggttgctga acaagaatta gacaaaaacg atctttctta
    13141 tcgcgtcatg cagcaggtct ttgattatta tcagttgcca ctgttcgagg atgaatatca
    13201 ttatatctat agtgatacat tcgacacgat tcaccagcaa gacgtcatga aactagttga
    13261 gaatgtgatt tataaagtaa gtaaagcaat caaccattct ttttatcaag atccacaatt
    13321 gttgacgaat ctgtatgctc atttatccat gcgattatcg agaaagaaaa aatttgtcaa
    13381 tgaatataat ccttttaaag aagatatcaa gcagaaatac ccggaattat ttgatgcgat
    13441 caagcaagca gttcgacaag aaataagtga acaaaatatt ctgatcaatg attctttcgt
    13501 ggcatatatt gccttacact ttttggtttc ctacgaaaga gaagaagatc tacgaagcgt
    13561 tagagccgtc tacatttgtt caacagggtt gggtgtgact agtctgatca agcaaaagat
    13621 caatgaagaa ttaaataata tcactatcgt ggcatttgct tcagtattga atgcgcaaga
    13681 aatgattgct accaaagatc ctgatttggt gattagtatt tttccaatta atggcatcga
    13741 tcgtcctttc atcaaagtcc atccattacc cactgaacag gacttgaaaa aaattcaaca
    13801 aataataaaa aaaatacttg cttcttccaa tcagcaggaa aggcataact tgaatcttag
    13861 agaaacgtcc atcaataaag atactttaga agactttagc aaggaattga ttttgaaagc
    13921 ttatacgatt tatgaacgtt tacttgtcat tttggaagca gaattgatcc aagaatatcg
    13981 agaagctttt ttgcttcatg tattcttaat ggttcatcgt atcacatttg atcaacaata
    14041 tatggcagaa ggtcgtcaag tagaccagat tattttagac aaaggaacac tggtaggaaa
    14101 aatcgaagca gtttttgcag aaaataattt aggaatcaat cagtcagaaa tcagtgcatt
    14161 attctcatat ataaaagaag gagcgtcaag ttagtgaaaa tgtcagacga ggctttaaaa
    14221 attatcgagg aaagtcaaaa tcgggaaaag ttaaaacaag tgattgatct aattgaagag
    14281 cgattagtag aacatgctat tgtaccaacc gagttgcagt ggaccattct aattaatcac
    14341 ttgaatgaga tgctgaaacg ttctcaaaga aaggaaacga ttcccgtagt cgatcgggat
    14401 ttgttcaaag aagtatccaa agaagccata actatttctg atgaaatagt tagaagtatc
    14461 ggtaatttaa ccgatgatga aatatatgtt ttgtcgattc attttgaaac agcaaaaaat
    14521 aatta
//
LOCUS       DAXWLF010000060        14388 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXWLF010000060 DAXWLF010000000
VERSION     DAXWLF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.060"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            108..1259
                     /locus_tag="ACGE4U_001375"
     CDS             108..1259
                     /locus_tag="ACGE4U_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8393549.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(1368..3407)
                     /locus_tag="ACGE4U_001376"
     CDS             complement(1368..3407)
                     /locus_tag="ACGE4U_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFF8393550.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLYAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3465..4022)
                     /locus_tag="ACGE4U_001377"
                     /pseudo
     CDS             complement(3465..4022)
                     /locus_tag="ACGE4U_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(4170..4336)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(4561..4767)
                     /locus_tag="ACGE4U_001378"
     CDS             complement(4561..4767)
                     /locus_tag="ACGE4U_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393551.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(4783..5397)
                     /locus_tag="ACGE4U_001379"
     CDS             complement(4783..5397)
                     /locus_tag="ACGE4U_001379"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFF8393552.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(5544..6227)
                     /locus_tag="ACGE4U_001380"
     CDS             complement(5544..6227)
                     /locus_tag="ACGE4U_001380"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFF8393553.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(6336..6794)
                     /locus_tag="ACGE4U_001381"
     CDS             complement(6336..6794)
                     /locus_tag="ACGE4U_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFF8393554.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(6878..8641)
                     /locus_tag="ACGE4U_001382"
     CDS             complement(6878..8641)
                     /locus_tag="ACGE4U_001382"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFF8393555.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(8862..10322)
                     /locus_tag="ACGE4U_001383"
     CDS             complement(8862..10322)
                     /locus_tag="ACGE4U_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393556.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(10530..11456)
                     /gene="glsA"
                     /locus_tag="ACGE4U_001384"
     CDS             complement(10530..11456)
                     /gene="glsA"
                     /locus_tag="ACGE4U_001384"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFF8393557.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(11479..12906)
                     /locus_tag="ACGE4U_001385"
     CDS             complement(11479..12906)
                     /locus_tag="ACGE4U_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8393558.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(12937..14151)
                     /locus_tag="ACGE4U_001386"
     CDS             complement(12937..14151)
                     /locus_tag="ACGE4U_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFF8393559.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4100 a         2917 c         2460 g         4911 t
ORIGIN      
        1 aaaaatcttt tggattgttg gtttttttgt gagtatattt tcctattttt tcaataatgt
       61 atgttatgct aagttcgggc tatattttat acaaaggaat gatgagaatg gaatttattg
      121 gtattttatg cctgatattg atttcaacaa cgattggctc acatatctcg cggagattag
      181 gtatcccagc agtgattgga caattacttg ttggtgtttt gctaggccaa gctggattga
      241 attgggtcaa acctgatatt ctagtccatg acttttcgga gattggtgtg atcttattaa
      301 tgtttttagc tggtctggaa agtgatttat ctcttttgaa aaaatatttc cgacctggta
      361 tgttcgttgc tttgcttggg atcatcttcc cggttgtttt tggctggttg acaggcaaag
      421 cattccaagt ttcgggaaat gaagcgattt tctttgggat tattcttgcg gcaacttctg
      481 tcagtatttc agtagaagta ttaaaagaac taaacgtagt gaatacaaaa gaaggatcaa
      541 caattttggg tgcttctgtg gtagatgaca ttttagtcgt tttagttcta agtttcagcc
      601 tatcttttct aacgggggaa gcttctagca atgcttcttt accgcttgtt ttgattgaac
      661 agctaattta ttttgttttt attttcttat tagtcaaatg gattgcgcct ttcctacttt
      721 catttgcaga aaaagtgtac gcaaattctt ctattatcat cgtatcgcta gttatttgtt
      781 taggaatgtc ttacgtagcg gatcttgttg ggcttagctc tgtcattggt gctttttttg
      841 ctgggatagc tgtcagtcag acaaaggtta aagaagaagt ataccacaat gtagaggctt
      901 taggatatgc agtatttata ccagtatttt ttgtcagtgt aggattagaa gttgattttt
      961 ctgactttag tgaacaacta ctctttattt ctatattgac gcttgtagcg attttgacca
     1021 aattgcttgg aggttatttt ggtgctcaga ttgccggttt ttctcaacat agttcattga
     1081 tggtcggtgc tggtatgatt tcgcgtggag aaatggcttt gatcatttta caaattggac
     1141 agcaaagtca attgatcgaa ccacagtact attcaccttt agttatcgtg attttattaa
     1201 gtacacttat ttctccactt gttctcaaat attttacaaa aaaagtttat gaagtgtgag
     1261 taaatcaaaa agaagttgtg aaagagacat cgtgaaaacc gtttatttga aaaaaggact
     1321 gtgacgataa cgccacagtc cttttactgt acttaaaaag caatttttta ttgataattt
     1381 tggttgaggt attgcaatag ttccgatgct cgtttggttg ctgaatcatc atcttcttta
     1441 ttttccaaca tgctgaccat catataacct tggttatcaa gattgaaagc atacaagaaa
     1501 ctgttctctt gcccctttac atcctgtttt tctttgattt ccgctgtacc agttttcgct
     1561 gccaacggaa tccctaaagc agaaagagaa tgtgctgtac catttacatc ttgcacaact
     1621 tctctcagat ctggcacgat cgtttgtact gctgtttcgc tgatgacatt cttcttatct
     1681 tttgtctctt tatctgcaat caatttagga tagacaagtg tgccattgtt ggtaaaaaca
     1741 gaatacattg ctgcttgctg gataggagta attagaagtt cgccctgtcc atatccagta
     1801 tcagctagca agatatctga gttaaagcta tcttcattag aaatttgtgc tggattcata
     1861 ctaattggca aatcaaggtc ttcaccaaaa atgaatttat ctaagcctgt acgaaatttt
     1921 ttctcaccca ttttcaacgt ttcttgtgcc gcatatatat tatcggaata gatcaaagca
     1981 gtttttaagt ctacttgtga tacatcacta ctgacacgcg ttgttttaaa agatccccaa
     2041 gaactgtctt tttgccattt aagcccgttg atcgtcaaca cttcatttgg atcgatagtg
     2101 ccgttgtcga gaccgattgc tgctgtaatc attttaaacg tagatccagg agcataacct
     2161 gtcgcaaatc ggctgatgaa tggatgttca ggattttctt cataagcttt gtaatcttct
     2221 tgtgagatcc cgtttgtcat tttgtttgga tcatagcttg gagagctagc aagcgcaaga
     2281 agatcaccgg tttttggcgt tgtcgcaaca gttgatgcag cttttcctcc tagactgtca
     2341 aaagctattt tttgtgcctt cgcatcgatt gtcaatttga tatctttgcc attttgaact
     2401 tcatgttcga tcagaacttt tttctctaca ccgtctgcat ctgtaatgct taatttccca
     2461 cctgttgtcc cacgaagatc cttatcaaaa gccatctcca aacctgagcg accgattttt
     2521 ccattactgc ttaattctgg attcttatca atatcttctg ctgtgatatc tcccacgtaa
     2581 ccaatcagct gagcagctgc ttcacccaaa ggataatatc tgccgtctac ttcttgaatg
     2641 gtagctccag ctggaagttc tggcgttgct ccatcaatga ttttcaatgg gacaaagtaa
     2701 tcgggttgta cccagctttg tgaaatcgcc tgattgatag catcttctgt taagtcgaag
     2761 gaggaagcaa tcgctttgat attggctgtt ttttcgcctc catctccaag tttacttgga
     2821 acgactccta attgttttag tttgcctgtt gttgctaatg gttcaccatt tcgatctata
     2881 atattccctc ttgctgcttc ttgtgtggtc aggcttactt tgtcatttcc ttccatttct
     2941 ggaaaaacca agttgggttg ccagttgatc gtggtttgac catcatttct gtctaatgtt
     3001 cctttataag aaagatcttt caattcacct aatgaggtat tcatctttgc tttatatgaa
     3061 aaactgtaag tagaatcgtc ttttttatct acttttagat tcgatatctg aagtcctttg
     3121 acatcagcag caccgtatat attctggtat ttatctaaga tctctttttc agataatgca
     3181 cttttatttg ccgccttttt tgatgtcatt tctgcagctt tgttataatc tcccttgttc
     3241 aaagcttgga caaattgctc aactgtcttt tctccagctt ctactgcttg ggtttcttga
     3301 tagtgctggt agtaaaaata accgccaccc gcagctgcta ttaagatcac tgcgccggca
     3361 atataagcgc ctgttcgatt tttgccgtgc ttgtcacttc ttttcatttt ttcctccttc
     3421 tcttgcttgt cattccttta tttcactatt tatacctgtt tattctaaca tatgtctttg
     3481 aggaaatcca taccacaatc cagaagcaca ctttctggaa ggttcgtttg gatactacca
     3541 agtaatttcc caaccgcttt gggtagcttg aatattgaag ttacgtcttt cagctgctga
     3601 gagatagctt tgatcacttg ctgctgcctt ctgatcctcc cgaagtctcc ttctttgtct
     3661 tcccgaaatc gagcatactg gagtaaagta ttaccatcca tcacttgctc tcctttgttt
     3721 atatatacgc catctaaatc tagagatttt tctgcatcga tcttgactcc accaggaaac
     3781 agttcattaa cgcaatcgat aaaatcttga aatgtgatac ttgcataata gggagcttca
     3841 aatttgaaat tttcctttaa tgtttgattt aataaatcaa ctcccccata agagtaagct
     3901 gcatttatct tattcattcc gtaacctgga atatcgacat atgaatctct catgatagag
     3961 atcagttttg cttgttgtgt tttctggttg tattgtgcaa ccagaagtga atccgagcga
     4021 cctccaccaa tcgatctttt ttcaccgcat ctcctcctca cacagcaatc cacttgtcag
     4081 gtgaggagcg agtttgtctc atcaaataag tcagtcacaa aaatataaag aaaaggtcag
     4141 aaaccttttc gaacaagaat tctgacctag catcatctca tcgtccgagt tttagcacta
     4201 tacaacgtaa agaaaacatt cttagctatt ggaaaaagcc ttaatccaac agtttctgtt
     4261 ttacccattg gcgactttcg acgtttctgg gcaacagcct atgtttgtat aggagcctca
     4321 cctaacaagg ttattactat attctattta cgagttcatg atagactttt cctaatgatt
     4381 aggcaatgga atttgaaatg aaatagtgaa atttaacaat ctttaatatt ttttagaaaa
     4441 aaataacaaa taagttgaaa gaatcccgtt gcaaattctt tcagttgcac caggattctt
     4501 tgataatttt ttagttttat gtgttttttt atatgttttt tcacttttcg gaataaatat
     4561 ttatttgtta aagtaattaa acaatccgct aatcgtttga tctggcattt ctatttcctt
     4621 accatctaaa gtaacagtag taacgacaaa atggtcttgc aatggcggaa cttctaagta
     4681 ctgcccttgc agaaaatcat tcactgtttc atattctttt ttcatttctt ttgaatctgt
     4741 cgatggttta tagcttattt ttaacataac tagtgctcct ttttattcgg gacgtgcaat
     4801 cgtgaaaatt tctcctattg ctgcataatc atttcctgcc aaacggctta cagcattcaa
     4861 ttttcttgga tcgatccttc ctgtcttatc atcatagact gtttcattta aatgatattt
     4921 ctgtacttct agcaaaaata aatcagctgt tactttattt ttattccgta tctcgacatg
     4981 ttcatataag gatgtttcaa aacgaacttt tgcttctgtc aatgctggta ctgaaatgat
     5041 tactgaatca gttaaagtca agtcagtcag gtacagttcg ctttcctcat taggcagatt
     5101 ggctgctgtt ttatttactt cttctacgtt gtcctcatca aggatatgga taacagcttc
     5161 tcttgtctca agaatattct ttgctgtgtc ttttactgca tcttgttttc tctggatcgc
     5221 caaggataag atgggtcgat tcgaactgac gatattgaag taactgaagg gtgcgatatt
     5281 cactatatct tttgctgatt tagtcgttac tactgctact ggtctaggga tgactgaacc
     5341 aatcagcaac ttgtaatttt cacgttctgt caattctgaa ggactccgtt caaacatggt
     5401 ttcgccttct ttctataaaa ttcgttaaaa agcagatctt gaagacaaaa tgaaaagtaa
     5461 tgcttttttc atcttcttag aatctgcttt aaactatcta tcaatttttc ttttttgtca
     5521 agaatctgtg ccgtgcgtgt tttttagtta tttgctttac ttgcggcttc gacatatctt
     5581 ttagtgatca attgcggacg ggtgataata gatccaacta cgactgaaaa agcacctaat
     5641 tcatatgctt tctctacttg ttcaggagag tcagtatgtc cttccataat tactgggata
     5701 tcgacttgtt ctatgatttg attcaataaa tcaaaatcag gtgtctgcgt ctttcgtgaa
     5761 tattctgtat agcctgctaa agtcgtagac aaaagatcaa aaccatcttc ttgcgcttga
     5821 agcgcttcct caaaagtcga aatatctgcc atagctaacc gaccgtgttc gtgaatcatt
     5881 tggacaagtt cagcagttgt tttttctgtc ggacgtttac gagaagttgc atccagtgca
     5941 atgatttctg cttgcgtctc caaaagttct tccacttctt tttctgtcgc agtaataaac
     6001 acttctgacc catcgtaatc tcttttgata atccctatta ccggtaaatc aacgattcgc
     6061 ttgatttcat tgatatcttc tgtcgtattt gctcgaatac caactgctcc gccttctttt
     6121 gcagccaaag ccattctccc cataataaaa gaactgtgta gcggctcgtt ttccaaagct
     6181 tgacaagaaa cgacgactcc gcctttgatt tgttccagtg tatccacaat atctcctcca
     6241 gtaatgttat ataaaaatac agcttcatat aaaaatgtaa cccttttcta ttcaggagac
     6301 aataatgagc tatttctttc agcagacctt tcttactaaa agaaaattac ttcaaattct
     6361 tcgtatacga ccagcttctc ttctgtaaat tctatgttca acgcttcttt ataagcatgt
     6421 ttaaaaaact cagcatggac tttccgccaa taagctaatg tccgatcccc ttctccttct
     6481 ttccaagcat gctctttcgt gacttcacta aaaggcaaga tggtcacatt tatatttttg
     6541 atgatacaaa ctggttcatt ttttccattc aggaccacgt ctaattgacc aacttttggt
     6601 aaaggctcat tctcttgttt atacagatcg taaccacttg atgtaccagt ctttatcttt
     6661 tttacaacta gttcgctcaa ctcgtctgcc attttttctg tatccccaaa tgaaaagaaa
     6721 cctaattttt ctgattgata actaggatat ttttcttgga actttttgac atattcttct
     6781 acttgctgat acataacatc cacccctttg ctttttataa tgaaaaaacc atccagatac
     6841 tagtataaac aattatctgg atggtgagca taatgaatta ttgaatattc aagacacgca
     6901 gactatcttc aacgatttgt tttggtgtta aatgataacg ttcataaagc tgccctacag
     6961 gaacgctatc cgtgaattct ttctctgcac caaaatttaa tacgttcatt gaagaatcac
     7021 cataataacg actgatattt tcgccaaatc ctcctgacaa actaccatct tctaatgtga
     7081 tgactaattc gtgtgtttct tttaatttat ctaatgtttc tacgtcattt tctgtgatcg
     7141 ttcctggatt gatcaacgtt gcttcgattc ctttttttct aaaagctttt actgtttctt
     7201 ttcctaattc aagaaaaccg cctaaagcta aaattgctac ttttcctcca ttatttactg
     7261 tttgataggc tggaatacta taatcatttg cgatagctgt accatgttct acaccatgtg
     7321 taggaattcg gattgctaca ggctgcgctg tttgttcgaa cgcccaatct agcatagaga
     7381 tcatatcctc ttttgttgct ggagcaagat atttgatgtt cgggatactt gataagtaag
     7441 gaatgtcaaa tgttccttgg tgtgttcggt cactagctga gatcgttccg ccaccgacta
     7501 aaataatcac tggattctcg ttgatcgcta aatcatgtga taattgatca tatgcccgtt
     7561 gcaagaaagt acttgattgg aagacgactg gacgtactcc ctgagaagcc aaagcagaag
     7621 cgaaagtgat agagtgctgt tcagcgatcc ccacgtcaaa ataatgatct ggatgttttt
     7681 cttggaatgt tttcagtcca aaggcgcctg gtatcgctgc attgactgcg gcaataggag
     7741 ctgtacttgt ttgcattttc ttatccaaat aatccatgat gatcatatta aatgtttctc
     7801 cagaagttac ttgctttgcc tccccggtgg acagatcaaa tggtacatgc caatgatatt
     7861 tcattttatt atcgattgcc ggctgataac cgcgaccttt ttccgtatgg acgtgaagta
     7921 caatcggatg atcaatatct tttacttctt caaataaacg gatcaccgtt tccagatcgt
     7981 ttccattatc cacgtaacga taatcaaatc ccattgcttt aaataggtta ttgctgcttt
     8041 taccgttggt ttgacgaagc tcagctaagt ttttatataa actgccatga ttctcatcaa
     8101 tagacatctc attatcgttt actaatacga ttagattgga ttgtaaaaca ccaccattgt
     8161 tcaatccttc aaaagctaag ccaccactta gggagccgtc accgataaat gctaccacat
     8221 ttcctgattc tttcttcaga tctctcgctt tagctaaacc tgttgctaag ctgatagatg
     8281 tagatgtgtg ccccactgta aaatagtcat gtttactttc atgttggttg gaatatccgc
     8341 tcacttcacg gtaatgtcct ttttcaaaac cattttttcg accagttaaa agcttatgac
     8401 cataagactg atgggaaaca tcccaaacga ttttatcctt tggtgaatca aatacataat
     8461 gaaacgcaat cgttaattct actacaccta gattcggacc aacgtgccca cctacatcgc
     8521 tgtccttttg caagattttt tggcgcatct cttctgccaa ctgtttcatt tcctcaacag
     8581 ataatttttt gacatctgcg gggctattga ttgcatccaa tacatgtaat actgtactca
     8641 taatacattc tctcctccgt actatcttct tttaatgaaa atagatttgt tacactttat
     8701 ctattctact acttagagtt gactctaagt caatagcatt ttaaaaaaat ttcagagaaa
     8761 gcttgttata taagtagatc ttcgcacgaa tcaggactca aataaaaacc ttctacagac
     8821 aattaaaaag ctttgtagaa ggtctcctaa ttaaggtctc atcaagaaat atgttttttt
     8881 aataggtgaa gttcctgagc aatatgatac aaatggatgt ttgcaccttt taatgagatt
     8941 ggatagatct ttgtcttttc agtttctaat ccattttgat agctagcagc atcgcacaaa
     9001 accactcttt ttttcgcaca tagacattca tcaggcaatt cttctaatga acgaaataaa
     9061 ataggattta tttcagcgat gccttcttcc gcatacaaat gaaattgggt tcgataaaaa
     9121 aattgtttga ctgaactttc tgaaacaaca aaaatttcta tttcctgtct ccccgcttgt
     9181 tcactgttga tcaaagaaag acttttgacg aattttcgaa tgaaattttc attccatctt
     9241 agttggattt gatttttctt tttccactct tccaatattt ctacaatttt tccgtacaga
     9301 agttgttctt tttctgatag aagatagatc ctttccggta aaaatacttg tccatctgcc
     9361 cacaacgtac ggacaaattg taaaaaggat tgcttgaaaa taaaattttc tgcatcgtcg
     9421 atttcagcga tactaataat ctgtaaaatc aattcattca gtgataggtc attgagaaca
     9481 aaatttttat aaacaacttc gaaatctttt cttaacagct ccatgttctc attggtataa
     9541 tttctactgt tgaacaaaga aaaaatataa tagacatctt cttcttgtaa gtctagaatt
     9601 cctttttgct cttccaatcc ttgacagact aattgaaata acggcttcgt ttctgcgctt
     9661 ttcttatctt cttctttcag tccaagggga aatgcctttc ttccgattgc gatctctatt
     9721 cctcgtatga taaaggactt ttcatcttcc gaatatcttc ttttaaaaaa atgagtctct
     9781 acataatcaa tcaactcgtt acaagcttct tctacatacg gatttttatt ttcatagcct
     9841 tcccaattga cataacgtac taaagctagc aagatgttcc gcgagtcaaa ttctgggata
     9901 atgatttctt tatttttcaa ataatcattt tctttaaaaa aatccaatac gatttttttg
     9961 attgtatata ctttgctcaa agaaataaat tcaagatcag ccagttctgt actcgtgaat
    10021 ataccattaa aataataact taaaaacttt aaaaaataag attgaccgta cagaaactgt
    10081 gtgagctcag aaagagcaat accatgtttg aatacgagtt tatacttatg cttctctctt
    10141 acttgttctt ttatacaata ttctagacac tcggctagac tttctagatc gtgaaggatc
    10201 gttggtgtag tcacccttag agaaatagct gtttgttcga tgtctatttc ttggaattca
    10261 aataattgtt cacacagata cacttttcta aatatatttc gctcgatgta attttccagc
    10321 attcctgata cacgcctcca gtttttctta tgattttatt taatcatatc tgtaaatcgt
    10381 atttttgttt cctttttatt tagaaaagcc ctttcaaaaa ccaataaaaa aagaaactgg
    10441 gacaaaagtc aaacaagctc acaatttcag cctcattgta ggtagctttg agccgtttgg
    10501 acttttaccc cagcctcttt caagaatggt tattcaaaga tgtcagcgtc tagttctttg
    10561 actacatctt tcaacaattg gataccagct gcgctgttac caaatggatc tagtgccggg
    10621 ctgaagacac ctatgccgta acgatttggc acagctgcca tcaacccgcc gcctacaccg
    10681 ctttttgcag gtacaccaac atggacggaa aactctcctg attcatcata caaaccagca
    10741 gttgtcataa ttgatttgac gattgtagca ctttcttcag taatgatttg tttgccattc
    10801 caaggagcaa ttcctttatt tgccagcaca gcagctaatt ttgctatgcc tgtagcggtg
    10861 acattgaggg agcattgtct gaaataagca tcgagtatct ccggtacgtc tccttcgatc
    10921 atttgattac ctttcatata gtaagcaaga gagcgattga tatcacctgt tcttgattca
    10981 gaatgataga tttcttcatt caatgtgata ttaggatcat cacagatctc tttcataaat
    11041 gacaagattc gctcaaattt ttcatctgca tctttaccgg caatcaacga ggttgttgca
    11101 atcgctcctg cattaacaaa aggattcatt ggatgtttgc gatgattgat ttccatattc
    11161 atgatagaat tgaaggcaaa accggttggt tccgtgttga ttgtttgaaa gactttttct
    11221 attccgttgt ctttgattgc taataacaat acgggaactt ttgacatgct ttcgatggca
    11281 aagcgcacat cggcgtctcc tccctcaatc agttgttttg tatccaaatc ataaatcgcc
    11341 atacctaatt gtttgggatc cacttcagct aatgctggaa tgtaatccgc cacttgtcct
    11401 gtttgataat agtggaaatt ttttccaatc aattgttcta attcttttac ttccatttga
    11461 ataaccagct ttcatttttt atttttctgc atgctggaat ttatgacggc atgcgtagat
    11521 cgtaaatgga ataactaaga cgataagata agaaacaatc agaatcgttt cataaatccc
    11581 atgctgtcca gctgcgatac tagcaggtgg aacaaatgag atacaaaaag caaaaagtga
    11641 agtcaaaaga cctaaacaag caacgattac ttttacaact tttccgcctg gaacctgata
    11701 ggctctttca agactgtcat ctttaaaaac cagcttcaaa tagcccgcga aaaacataaa
    11761 gtacaacatc aggtagatta caacagtcaa tgacatcgct gcaagaaatg aaagattatt
    11821 ccccccgccg cttaatgtaa agacggctgc ccagattgtt gcaacgatcc catttgcgat
    11881 catcaaatga gcaggaacac cgtgtgtgtt ttcttttgca aaatattcag gaagtaatcc
    11941 ttcttctgct gctacgtgaa gtgcttttga aggagaggtt acccaagaag caatttctcc
    12001 gatgacacct aaacaaacaa gaatcgcaag aattttcgca atccatgcaa gatttggatc
    12061 aatgtgtctt aataggaatt ctaacgattg aatcacccct gtattaagag acaaaccatg
    12121 ttgaggtact gtagtagcga cggtcaatcc gccaaaagta tctaaaatga ttgccagaat
    12181 aaccaataca aacattgcta atggataatc ctttttaggg ttttccattt catttgcatg
    12241 actggcagat gtttctacgc ccatatatgc taaaataaag gaaacaaaaa caaccagcgt
    12301 aggcaatttc gaaaaatcag gaatcaatgt tttcatagac agtatcgttt gtagtggagc
    12361 gcctgaagcg acataatgga tccctaagcc aaacaagact aatgctggca acaagatacc
    12421 agctacaaaa ccgatccgag caatcaatga agtgtatttg gttcctttta actgtgagag
    12481 taaaatagcc cagaaaataa tcaataggat tgccaatttc agatatttat tgttttgtat
    12541 agcaccaata cccgttgcat aggataatgc accagttaag aaatacagca tggtcaagta
    12601 gccaactgtt atctcaaacc attggaaaaa gattgcggca aaaccgaatc gttttcccaa
    12661 tgtgcgactg acccaagtat agacaccgcc tttttcccat cctttcaccg ttgccatctc
    12721 tgctgcgcac aatgccacag ggataaacca tagcaaaccg cctaataaca agaaaaatac
    12781 taagctgaag cctgaggtcg cgaatgtggg atattcatat aatgaaacga ccattgagat
    12841 ggtcatagcc aaaaagccaa agaacgttaa tttttttgca ggaattttcg ttttgctttg
    12901 ttgcatgaat cttcctcttt tctatttgaa tttgtttcaa ttgagtttgt tgccaaagaa
    12961 gaaaaagtcc aatccttctt cttgttcttc ttttgtagta gtgatcgttg taaagtgcat
    13021 cgccgatttt aaaatcactg ttgtcagcac ataggcatac acggcagtaa tgatgacacc
    13081 aatcacttga atccctaaga agtaccagct gcttggattc gttagtaaat ggctgttaga
    13141 tgcaaagatc cctatcagaa tcgtacctgt gaatccgccc atgccatgaa caccccacac
    13201 atcaagtgca tcatcccatt gtttcttttt acgaaaatca acagcagcat gacagacgat
    13261 acccgcaatg atcccaatca gcatggcact ttttgcttct acataaccgg cacaaggcgt
    13321 aatggctgcc aatccagcaa cggatcctgt tagtacgtcg acaaaatcaa agccatttcc
    13381 ttttactttt gcaatgatca gccaagtaat catcgcaaat gcaagagcga tgaatgttga
    13441 accaaatgca ttgactgctt gttcatctgc tcgaagtgca ccaccggaat taaaaccgaa
    13501 ccagccaaac caaagcagtc ctgtaccgat agcagctgcc ataagactgc tatgctgact
    13561 tctttctcct aaaggaatct ttctttgacc aagtgtgagg atacatgcta aagagccgaa
    13621 tccagctgtc gtgtggatga ctgctccacc agcgaaatct ctaaatccca tctgatccaa
    13681 aaatccgcca ccccatatcc agtggcaaac agggaaataa atcagcagat tccaaaaaat
    13741 aacaagtata atatagcctt ttaaattcaa tctccctgca aaagctcctg tcatcaaagg
    13801 aacggtaatc acgataaaca tcagttggta aagaaagaac attaaaaatg gaatcgttgc
    13861 accatgaaac atgttgggga agcttgtaac atgtctcatc aaaaagaaat ctgccgggtt
    13921 tccaataaca ccgccgatat cccgtccaaa ggccaatccg aatccaccaa atacccacat
    13981 aatcgttgtt atcccaacgg agagaaaaga ttgagacatg atcgttagta tattattctt
    14041 ccgtgctaat ccgccgtaaa aaaaagctag accaggagtc atcaggcaaa ccattgctgt
    14101 acataaaatc atgaatgctg tatctcctgt attgataggt aatgtattca tttttttcgc
    14161 ctcctttcta cgattacgag tataaaagaa ggtgtagttg tttttttctt ctgttttttt
    14221 gaattaatac ttgcatttat aaaagacaga caattaataa aagcttgatg tgttgtgctc
    14281 tttttcgttt ttcacacaaa taaagacaaa aaataaacgg tcttcttttt atagaaaacc
    14341 gtttattttc gtcttttttt atttttaaag atattttttc ataaggaa
//
LOCUS       DAXWLF010000061        14352 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXWLF010000061 DAXWLF010000000
VERSION     DAXWLF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.061"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            26..1765
                     /gene="yfcC"
                     /locus_tag="ACGE4U_001387"
     CDS             26..1765
                     /gene="yfcC"
                     /locus_tag="ACGE4U_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFF8393560.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1922..3106
                     /gene="iadA"
                     /locus_tag="ACGE4U_001388"
     CDS             1922..3106
                     /gene="iadA"
                     /locus_tag="ACGE4U_001388"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFF8393561.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3217..3420
                     /locus_tag="ACGE4U_001389"
     CDS             3217..3420
                     /locus_tag="ACGE4U_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFF8393562.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3521..4183)
                     /locus_tag="ACGE4U_001390"
     CDS             complement(3521..4183)
                     /locus_tag="ACGE4U_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393563.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4196..5698)
                     /locus_tag="ACGE4U_001391"
     CDS             complement(4196..5698)
                     /locus_tag="ACGE4U_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8393564.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6246..7925
                     /locus_tag="ACGE4U_001392"
     CDS             6246..7925
                     /locus_tag="ACGE4U_001392"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFF8393565.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8052..8531
                     /locus_tag="ACGE4U_001393"
     CDS             8052..8531
                     /locus_tag="ACGE4U_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFF8393566.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8750..9430
                     /locus_tag="ACGE4U_001394"
     CDS             8750..9430
                     /locus_tag="ACGE4U_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFF8393567.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9438..10769
                     /locus_tag="ACGE4U_001395"
     CDS             9438..10769
                     /locus_tag="ACGE4U_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFF8393568.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10784..11746
                     /locus_tag="ACGE4U_001396"
     CDS             10784..11746
                     /locus_tag="ACGE4U_001396"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFF8393569.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11857..12195
                     /locus_tag="ACGE4U_001397"
     CDS             11857..12195
                     /locus_tag="ACGE4U_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFF8393570.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12274..12981
                     /locus_tag="ACGE4U_001398"
     CDS             12274..12981
                     /locus_tag="ACGE4U_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFF8393571.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13084..>14352)
                     /locus_tag="ACGE4U_001399"
     CDS             complement(13084..>14352)
                     /locus_tag="ACGE4U_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8393572.1"
                     /translation="TDQTKKLAVFSKLKLCYLKESNRFNYLMLLINYPQTAVPKKYLL
                     EQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIRLYLYFFLSDTFGG
                     NDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQQAYFPKTSSEITE
                     LMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQISFRQKQEIGFLF
                     STSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTL
                     CFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLI
                     HLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDT
                     THNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4563 a         2490 c         3111 g         4188 t
ORIGIN      
        1 tgaaaaaaga aaggaagaaa taaatttgaa gaaaacaaat tcttcaggaa aaccgaaacg
       61 cgacttcact aaattatcga caccccatac gtatgtcatt atttttggtg tagtcatctt
      121 tgcatggata ctgacttttg tcgttccagc aggaaagttc agcacgcaag atattgaata
      181 caaagatgca aatggcgaaa caagtactcg tactgtttta cgtcaagatt ccttccgtta
      241 tgcttacgag ctggataagt cgtatgtgtt tgatcaatta gaagaactgc aagatcatcc
      301 agcagaacgt gaaaaactgg atgttccgga aaaaggattg gaaaaagtca ttgctgacgg
      361 agaaaaaaat cttacgcagg aaaaactaga tgaaatctcc ttgacagatg atgtgcttta
      421 cgatcagtac ggcgaaaata tctatgatac ttctaaaaaa ttgcataaaa cagccaaaat
      481 atggggaaca gatgattttg gcggctttgg tttcttaaat tttgtttttg aaggactagt
      541 atctggagat aagtatggat ctgctgtagg gatcgctgca ttgatccttg ttgttggagg
      601 tgcttttggt attattatgc gtaccggagc gatcgacgca ggtatctatg cgtttatcag
      661 taaaactaag ggactggagc gtttagcttt accgctatta ttctttgctt tttcattcgg
      721 tggcgctaca ttcgggatgg cagaagaggt aattccattc tcaatggtga tggttccatt
      781 tgtcattgcg ttaggatatg actcgatcgt agcagtaacc gtgacttatg tcgcatctca
      841 agtcggaaat gcgacctctt ggatgagccc gtttagcgta gcggtggctc aagggattgc
      901 tggaatcccc gtgttgtctg gtgcgacatt ccgattgatt atgtgggtag ttgttactgc
      961 attagccgct ggttatatga tgatatatgc agaaaaaatc cgtaaaaaac cagaaagttc
     1021 gttgacttac aaatcagatg attatttccg ttctcatatc aaaaagacat ctgatgaaaa
     1081 taaaaaattc atgcttgggc ataaattgat cttattagag atgttagttg tccttgtctg
     1141 gatcgtttgg ggcgtgactc aaaaaggcta ttacattcca gaaatcgctt ctcaattctt
     1201 tgtgatggga ttagttgctg gaatcattgc tgttcttttc aaattagatg gaatgaaagt
     1261 caatgacatc gcttcttctt tccaatctgg tgcagctgat ttagctggta cagcaatcgt
     1321 agtaggtatg gccaaaggga ttcttcttgt cttgggagga tctgatgcat ctgttccttc
     1381 agctttgaat acgattttgc atgggatcgg gacggcgttg accggtgttc cagctgtcat
     1441 tggtgcttgg gcgatgtaca tcttccaaag tttgttcaac ttggttgtta catcaaattc
     1501 aggacaagca gctttgacta tgccaatcat ggctccactg gcagatttag taggtgtatc
     1561 cagacaagta gcagttcttg cttatcagct tggttcaggt tttatggacg cttttacacc
     1621 cgtatcagcc agcttgattg gtgtgctagg tgttgcacgg atcgaatggg caaaatgggc
     1681 aagattccaa atcaagatgc aaggattcct gtttgtttta ggaactattt tcatcatgat
     1741 tgccattgca ataggtctgc agtagtactg gagacctgta aataaaaaag taatccaaac
     1801 agtagataag gactccaaga accgtcattt tacggtaaaa tcaaaggagt aaagatccta
     1861 tcgtttggat tattttttaa gatcctatca tcagttatac ttaaagaaag aggaaaaatg
     1921 tatgaaaatc attcgacaaa ttgaagtgtt tgcaccagat tacttaggaa aaatggatgt
     1981 actgattgcc ggtgggaaga ttttggctgt agaagaacag ctagaaggcg gctacgaagg
     2041 agtggaagta gaagaacttt caggtgaggg aaaagtacta accccgggtt tcattgactg
     2101 tcatttccat attttaggtg gaggaggaga aggcggttat caaaatcgaa cgccagaagt
     2161 cacattgagc caattgacta cagctggtgt aacgacagtt gtcggttgtt tgggaacaga
     2221 cggagaaggt agagatatga ctgcgttgat cagcaaagcg aaaggtctgg aagcagaagg
     2281 aatctccact tatgtatacg aaggttcgta ccgattgccg gtaaaaacag taacagattc
     2341 tatcatcaaa gactttttga caattgataa aattatcgga atcggagaaa tcgcagtatc
     2401 cgatcaccgt tcttctcagc caagttttga agagttcgct cgtgcagtag cagatgctcg
     2461 tgtaggaggc atgttatccg gaaaagctgg gatcatcaat gtccacttag gcggaggaaa
     2521 aagaaagcta gagctattga cacgaatcgt agaagaaaca gaaattccga tcacacaatt
     2581 tttgcctact catgcaaatc gcacaccaga attatttgaa gcttgcgtgg catttgccaa
     2641 acgtggtggc acgattgact tcactgctag cgaagatcct gatttttggg agaagacaga
     2701 tggtgaagtt cgcttcagca aagcactgaa acgattgatc gaggaagatg tttctttaga
     2761 taatttcacg atgacctcag atggacaagg aagcttgcct tattttgatg aaaacaatca
     2821 tttcctaggg ttaggagtag gaagtgccaa ggcattgcta gtagggatca aagaagctgt
     2881 gcaaaaagaa agtatccctt tggaaatcgc cttacgagct ataaccagta atccagcacg
     2941 tatattgaaa ttggataaaa aaggaaaaat cgaaattggt gctgatgcag atctgtgtat
     3001 cttggataaa gaaacgcttg atattgatac agttatcgct aaaggagaaa taatggtcca
     3061 agaaaaagaa gtcaaagtat gggggacttt cgaaaaaagt ttttaatcaa cagctctaac
     3121 tatctttttt agaaaacgag cgttctgttt gtctgtatct gttgttgatg ttaccctgaa
     3181 ggtagatcaa taaacaaact aagaggagga agcacaatgg cagatttaaa aggacgttct
     3241 aaagatatga aagataaagt agtcggtgaa ggaaaagaag catacggcaa agcaacagac
     3301 gacaagagca aagaaacaga agggaaagca caatcactag gtgctgacat caaagaaaaa
     3361 gctcgaaatt taggcgacga agtcaaagac aagttttctc gaaaagacga aaaaaaataa
     3421 aaaccactta ttactaattg aatcaagcaa ataaaaaatc cgaaccatat ctgcgagaca
     3481 gatatggttc ggatttttta tttgatcatt atcctgtttt ttaaacttcg attttttgaa
     3541 attttttatt ttttagttca aagacttcat cacagctttt tgctacttca atctcatgag
     3601 tcacgatgat aatacagcga ttttgttcat gggcgatttc ttggaaaagt ttgacgatat
     3661 cttgtgaagt tgtttcatct agatttccag taggctcatc cgctacgatt agttgatgtt
     3721 cacagcagat agcacgaacg atagcgactc gctgctgctg acctcccgaa agtttggtca
     3781 cctgtttttt tgctaatatt tcatcaattc ctacacgagc cagtcgatct aaagcaactt
     3841 gtcgataatt ctcttgcttg gtcttagtga tttccattgc tgctatgacg ttgtctagag
     3901 ccgataaata aggaagcaag ttgtaagctt ggaaaataat cgagacatca tttttacgat
     3961 aacttcttaa cccgattttg ttcagtgaac gatcttgata gtataccttt ccttcttttg
     4021 gtgcatctag tccagaaata agcgaaagaa aagtcgtttt tccagaacca cttgctccta
     4081 aaatagcgta catttttcca gcttcgaaat tcagttctac atctttataa agcgcatctt
     4141 gcggatcttg ataccaaaat cccaattgtt ctgtccgtaa catcaaattc ctcctttagt
     4201 ttaataggat ttttcttggg tttaatctta atatccctat agaagctaaa aagacagaaa
     4261 acagactgat tccaaatgct actccggcta atagtcccaa ttgtgcaggt ttgacattga
     4321 tttccaattc gctgacttga tcagaaacag caaatggatt attcgtggtt ccttgttgac
     4381 catcgcgttg accgccttgt tcttgtctgt tacctggcat ttgtccaccg ccaggagctt
     4441 gctggttttg tgtcgtggtt tctgtctgtt ggtcaagtag ctgctgtccc acggcattcg
     4501 cgacgacgtt tccgctaagc gaagcaactc ctaatgcaag aatcatacaa atagccactt
     4561 cggtaaacaa ttggagaatg attttacttc tggattctcc taaagatagc aagacgccta
     4621 actcatgccg acgctcccga atggtaatca taatgatcag agtaagtatg ataataccgg
     4681 ctacagccac tagtaaaaca acatttttag aaaaacttgc cacgttgttt aaaggttcca
     4741 acattgactg atacatggaa tcatttgatt gtaagctaaa tgtatctgtg tcgatcagct
     4801 tctcagcttc tttgagaaac tcatccattt tatctggatc agatagagtg taaatagcag
     4861 aatctatcgt gttatcgtca gatgtcccat ttagttcatt tgcaaaagtg taagaagtgt
     4921 aaagtgtatt cgacggattc atgaagttga atttcatacc catactgccg cttgtttcgc
     4981 tggattcata aattcctttg atcgtcattt cataagtggt atcttcatct tcagtactcg
     5041 tgattgtgaa agtatctccg actgccaggt catttgtttc tgctagagta gaatcaatga
     5101 ctacattgtt tgtgcctttg tcgctgtcat ctattccttc accatccgtg atttttgctg
     5161 ttccatcaga aaaattacta ttttgtgcag ttgctgacac accactgatt tgaaagtctg
     5221 cttgagtcat ttgcggattc ccgcccattc ctccagcagg ttcaccgcct gcatcagtag
     5281 tttctgaact gtcttcactt gaatcagaac tagtgatggc agttattcca tcttttgcca
     5341 tagctgaggc ggaactttca aaggaataac tctttacatt atctaattca gctatttttt
     5401 ccgcgtccgt taaggaaact ggtgtaagag aaaaactgcc tggatctgga cgttcttcag
     5461 tggtagaacc gcttgaagaa gtttgttcct gttttttaaa tgaagcttct cgattggcac
     5521 ttaaagtcac tgttgctcca acttcttttt gtgcttgatt cgctgcttct tctgcagcgc
     5581 ttcgaatcgt gaggccagct agcacaaaaa taagaatggc tgtgaacacg gcaaacaata
     5641 acaatgaacg tccccatttt acttttgtac ttttccatgc acgtttcata aaattcatat
     5701 tttgcatccc tcctagtaga tgcatttgat aatagacaac ctatcttaaa gctagcttaa
     5761 atcttatgct gtttgcacac catcaacatg aggttctgta tatataaaaa gtcactatag
     5821 ttattgcaaa aaatgtggta aaatgaaaaa agtatgtttc aaaccgacga agaagaaatg
     5881 aaagaggaat agcaaaagaa atcctagatg ctacagaggg atttttacgt gcatagttgt
     5941 tctaacaatt gctgatacta gcgatttagt ttatttgttg tataataatg gggaacagct
     6001 gaatacttat ataagtattc tggctatagc gtttcgcaaa agatccattt tgttaatgaa
     6061 gtatatccaa aataaaatgc ccatccaact gtcagaacgc ggatgtgctg gtaattttcc
     6121 tgctgattct ccataagttg agaaatagtg attttgcata tgcttcattg gaatggctca
     6181 tatatttaga taaagaagaa aatcgacatc tttaaacttg gatgtcataa gaagaggtga
     6241 agtttgtgag taccattaaa attatcccat taggcggcgt tcgtgaaaat ggaaaaaaca
     6301 tgtatgtcgc agaagtgggc gaggatattt ttattttaga ttgtgggcta aaatatcctg
     6361 aaaatgagtt gttggggatc gacgtcgtaa tccctgattt tacgtattta gaagaaaaca
     6421 ttgatcgtgt agctggtgtc tttttaacac atggacatgc agatgcgatc ggggcacttc
     6481 cttacttgtt gtcaaaaatt tctgtaccag tattcgggac agagttgacg atcgagttag
     6541 caaaattgaa tgtcagtcgt aatgaagcag ccaaaggatt taaggatttc catgtggtag
     6601 atgagcatac agaaatagac tttggtgaaa cagtagtgag ctttttccgt accactcata
     6661 cgataccaga ttcagtgggg atcaatctga aaacaaaaga aggtagtatc gtttatacgg
     6721 gagattttaa atttgaccaa acggctatcc caatgtatca aacagattat gcacgattgg
     6781 cggaaatcgg caaagaagga gtcttagcct tactaagtga atcggcaaat gcagaaaatc
     6841 ctcagcctgt agcttctgaa gcccaaatag cagatgaagt atttgatacg atcaaatatt
     6901 gggaagggcg tatcatcgtc gcttgtgtag caagtaattt gcaacgagtg caacaagtat
     6961 tggatgcagc agaaaaagca caacgaaaag ttgtcttgac tggtcaagat ttcgaacgaa
     7021 ttattcgcac agcgatgcgt ttagaaaaac ttcaattacc aagtgaagat ctgcttgtta
     7081 cgttgaaaga catgaaaaac tatgatccag aagaactgat cattttagaa actggccgaa
     7141 tgggtgaacc catcaagtct ctgcaaaaaa tggcaaacgg tacacaccgt aatgtcaaga
     7201 ttgaagaagg agatttggtt tatatcacta ctacgccaag catcgcgatg gaaaccatcg
     7261 tggcgaaaac ggaagatatc atttatcgag ctggtggaac agtgaagttg atctctgaga
     7321 atatgcgcgt ttctggccat gccaatccta atgacttgca attaatgatc aatttgatga
     7381 aaccaacata ttttgtgcca gttcaaggag agtatcgtga attagctgct catgcagatt
     7441 tggctcatgc agtagggatg ccatacaaaa atatctatat tactggacgc ggagacgtgc
     7501 tggaatataa gaacaagcgt atgagtgttg caggaactac aagtgcagaa aatgtaatga
     7561 ttgatggatt aggcataggt gatatcggaa acattgtttt acgtgatcga aaaatcttgt
     7621 cagaagacgg gattttcgta gcggttgtca ctatcagtcg gcgagaaaag aaaatcattt
     7681 caaaacctca aattacttca cggggattcg tttacgtaaa agctagccgt gatctgatga
     7741 aagaaagcag caatatgatt gaaacaatcg ttgagaaaca tttagaaagc aatgattttg
     7801 agtggagcaa attaaagcaa gacatccgag atcaattagg ccgttactta tttgaacaaa
     7861 caaaacgccg cccagtgatc ttgccagtca ttatggaagc tactcaacgt cgaaataaaa
     7921 aataaacaaa aagagtaaga tgaacctctc tatagagtgc tcgtcttgct ctttttttgc
     7981 gaaaaacaaa taatttttga atacagcatc cctcgcttta taataaacga tagaataaag
     8041 ggagtgaatc aatgatgagt caaacaaaag tcgaacgtta tttagaagaa caaaagatca
     8101 gctatcaacc acttgatttt agtcatgtgg tagaagaatc tttcacagaa gaactacaaa
     8161 aaagagggat tgatccagct ttgatatgta aaactctcgt agtgaaagga aacaaaactg
     8221 gggtgatcat cgcattgatt cctttgaacg atcacttaga ttacaaaaaa acgagacaag
     8281 tgacgaaaga tcgtaaagtt ggttttcctg gaatggattt tgtattaatg catacaggat
     8341 atccccacgg agcaaacacc cctattggaa taaaactcgc acatcctgat tattgttttt
     8401 tagcagacag ttctttaaaa gaaaaagaag aagtaattgt gtcaagcggt gaaatcggga
     8461 gaagccttcg gataaaagta gccgatttag aaaagatcgt ccatcctatt tacgctgatt
     8521 taattagtta agggacgaga agagaagcta agcagagcag agacctcaaa cttgagatta
     8581 tatctgcttg gtacttggtg catcctgtat tatctgttac aattgctttg tacatactgt
     8641 actatcaatc caatgaaaat atatttagca gctatatttt ttcattgatg tgcaagtgat
     8701 gaaatcgttt ttaataaaaa agatacagag aaaacgcgag gtgtaaaata tgaaaattac
     8761 gtatcatgga cattcctgtt taacaattga aatggaaaat ggccaacgcg tgatgattga
     8821 cccgtttatt acaggaaatc cattgacaga cttaactgca gaagaagtaa agacagattg
     8881 gttgctgatt actcatggtc atagtgacca cataggagat atgattccta ttgcaaaaga
     8941 aaatcaagcc acaattatca gtattgttga gattgctgat tatgcgaaca gcagaggagt
     9001 agattcattc ggtatgaata tcggtggaaa acatgcgttt ccattcggcg tggtaaaatt
     9061 cgtacatgca cagcatagtt caagctatga agtagacggt atcgttcagt atatgggcga
     9121 gcctagcgga attattattc aagcagaagg aaaaacgatt tatcatgcag gggatactgc
     9181 ttatttcagt gatctaggat tgcttgcaga agaattcgat attgatgtcg ctttcttgcc
     9241 gattggcgat aactatacga tggggccaga agatgcagca aaggctgcgc aaacaattcg
     9301 tgccaagaaa gtagttccaa tccattacaa tactttccct gtcattgaac aagatcccga
     9361 atcttttgta gagttactgg aagatagtga aggaaaaata atgagcgtct cggaaacgct
     9421 ggaagtgtag gcagaaaatg gcgacaaaac atgatttgat tttagaatat attgaaagtt
     9481 tacctgttgg caatcgaatt tctgtaagaa gcatcgcaaa agaattgaat gtcagtgaag
     9541 gaacagcgta ccgtgcaatc aaagatgcag aaaatgtcgg actagtttca acaattcaac
     9601 gagtagggac catccgtatc gaacgcaaat taaaaaaaca tattgaacgt ttgacatttg
     9661 gcgaggtcgt caggatcatt gaaggggacg ttttaggcgg ggcggcagga ttagacaagg
     9721 tcttgaataa attcgtcatc ggcgcgatga ctaaacatgc catggaacgc tatattacgc
     9781 caggatcact aatgatcgtc ggcaaccggg cggaagtcca aaaattagca ttggaagatg
     9841 gcgccgcagt attgatcaca ggaggattca atacgactgc tgagattgca aagctagctg
     9901 atgatcttga aatgccgatc ttacgcacct cttatgatac atttacagtc gcaaccatga
     9961 tcaaccgagc gttaagcgac caattgatca aaaaagatat catgcttgtc agtgatatct
    10021 atatgccagc tgaaaagact cattatttgc agcagacaga tacgatttct gattatcagc
    10081 gtttgtcaga agaaacgcag cattcgcgtt ttccagtcgt caaccgccat catcgtttga
    10141 tggggattgt tacggcaaaa gatgttttag ggaaatcgcc aaaccagatc atcgatcggg
    10201 tgatgacgaa agaaccaatc agtgtaaaga aaacaatgag tattgcctct gttagccatc
    10261 aaatgatctg ggatgggcta gaagtcatgc cggtcgtttc cgatgacttg acattggaag
    10321 gcttgattac ccggcaagat gtaatgaaag cgatgcagct tgtccagcga caaccgcaga
    10381 ttgcagatac gatttcggat cagatatctg gaaatatcca tacgattgat actgaccgtg
    10441 aaggaaatcg tttggaaaac cctaagttca aattcgtagt agcaccacaa atggtcaatg
    10501 gcgttggaac catttctttt ggcgtactta gcgaaatcat ttctgatgtc gcacaaaaaa
    10561 caatggtgat gaaccaaaaa cgaaatatct tgattgaaca agtgaaccta cattatttgc
    10621 ggctgatcca attggaaagc gaattagaca ttcgtccacg tgtattggaa atcggcagac
    10681 gttccgctaa actggatatc gaagtgttta ttgaaaacac catcgtttcc aaagcaatcg
    10741 ttgtttgtca agtaatggaa cgttcttaga aagcaggagt gaaatgacag tacagcaaga
    10801 aatagtagat acaataaaaa aatacgataa aatcattatc cacaggcatt tgcgccctga
    10861 tcctgatgca ttaggatcac aagtaggatt agccgaacta ttacgggaaa gtttcccaga
    10921 aaaagaaata cgtcaagtag gtgaaacgat tgaagggctt cgctttttag cagaaatgca
    10981 agaagttgaa gatgtcttct accaaggagc gctagtcatc gtgactgata cagctaattc
    11041 acccagaatc agtgatcaaa gatatcagtt ggcagaaaaa ctgatcaaaa tcgaccatca
    11101 tcccaatgat gacccttatg gagatcttgt gtgggtaaat acaaaagcaa gtagctgtag
    11161 tgagatgatt gcagaatttg ctttgatgtt tcctgaatct ttaaagatga acgttaaggc
    11221 tgcacggtta ctttatgcag gaatcgtcgg agatacaggg cgctttttgt atccctccac
    11281 gacgtcccgt acattagaga ttgcttcgat attgagaaat tatccatttg atgcaagtgc
    11341 tttgaacaga gagattgaac aaatgccgat gaaagtcgct aaactttccg gttatcttta
    11401 tcaaaatatc caggtggatg aaaatggtgc aggaagaata atccttccgc aagaaattct
    11461 tgataaatat ggaatcgatg attcagagac agcttcgatc gtttcacttc ctggagtgat
    11521 tgacgaagtg ctggcttggg ggatttttgt tcagcagcct tcagggtatt accgcgtacg
    11581 tttacgttct aaggggccga tcatcaatga attggctaaa cgccatcatg gaggaggcca
    11641 tccattagca agtggtgcga atgcaaaaga tttagcagaa gtagaggaaa tctataaaga
    11701 gatccaagat atatgccaat cgtataagag cagaaaaaca gaatgatact tcgttcgaaa
    11761 acgtgccttt tgtagtaaaa atcacaaatg tatcttttca agatcttctt tttccgttac
    11821 aatagtgctc actaaagatt ttaaaggaga aagaagatga cattaccaaa ttgcccagaa
    11881 tgtggctcag agtacacata tgaagatcga ggcttgttga tttgcccaga atgcgggaac
    11941 gaatggaaca aagaagaaca agaaactgtc atagaagacg gattagtggt attagatgca
    12001 aatggaaacc gtctgttaga gggcgatagc gtgacagtga ttaaagactt gaaagtaaaa
    12061 ggtgcgtcag gtgccattaa acaaggaaca aaagtcaaga acatccgttt agtagaaggc
    12121 gatcataata tcgattgcaa ggtagaagga tttggaccga tgaaactgaa atcggaattt
    12181 gtgaaaaaga attaaagaat attcaaggtc ttttcacatt cttttcctac tactaatgat
    12241 aaaataaagt aaacgaaaag gagtgaaacg aaaatgtatg gttatggaat gtatggtttt
    12301 ttagatccga catttctttt ggtcattctt ggattagtta tttccggaat tgcgtcagcg
    12361 tacgtcaata gtactttccg aaaatacgat caagtgagaa gcaaaaacaa tgtgactggt
    12421 acacaagcag cacaatatat attacaaagt caacagataa ataatgtcgg tgttcaacaa
    12481 attgccggag atctcacaga taactacaat tcaggtaata agatgttgag tttgtctcaa
    12541 gcaactgcac agtctacttc agttgctgcg ataggtgtag cagctcatga atgcggacat
    12601 gcggtacaag atgcttcggg ttatgtccca ttgaagattc gtgcagcact tgttccagca
    12661 gctaactttg gttcgatgat ttcgttcccc ttgattatgg taggtgtatt gcttagctgg
    12721 aactctacgt taatcaatat cgggattttc gctttttctt tagcattact tttccaattg
    12781 gtgacattac cagtcgaatt taacgcgtct cggcgagcaa tccaaatttt gagtgaaggc
    12841 ggactactga cggaagaaga agtaccgatg gcaagacacg tattatttgc agcagcactg
    12901 acttatgttg cagcagcgct ttcgacattc ctgcagctgt tgcgcttgat cttattgttc
    12961 ggcggcaacc gaagagatta aaataaagaa gttgtgaaat cagcgctttg atctgagcta
    13021 ataccgagag ttagctgatt gaataccatt tatttggaat aagaggatgt gacaagaatt
    13081 ttgtcacatc ctctttgttt tgttctcata accttttttc atatacagtt catgaaccgc
    13141 ttttattaac ttgccaaata acactgtatt gtgcgttgta tccagataga aagtctccgt
    13201 ttcatctgaa attttctcat gctgatctgt aatgatcaaa tcagcatgtt ctgaatatgc
    13261 ggtaatttta acagcatctt tgcgaaataa ctgctgcaaa tggctttgaa gatgataatt
    13321 tcctgtaaac tgtttggtta tctgcaaatg gatgagtaac tgctctttcg tttctgtttg
    13381 tagtaagtgt tggatcaatt gactaaggta ttgactgatt tggtttcttt ccccattttt
    13441 tgaaaaaatt gtatcgattt tttctcttaa tttcttttcc tgtataaaag aactagaatg
    13501 attggacgtg gcttctggaa agcaaagtgt agaaaaacta ctgctggatt cacccaacag
    13561 ttcgaaaaaa acaactgcca acacaagaaa ataaaaataa attggtattt tattttctaa
    13621 atggttgaga ggaaactgtt gagttaagta agtcatcaat tgaccgactt gtcgacaaat
    13681 aaggtttttg cttgtggaaa ataaaaaacc aatctcctgt ttttgtcgaa aagagatttg
    13741 atttggaaaa aaacaataga aaaaataatt aaagtaggct ctctctgttt ctgtctcttc
    13801 acaagggcga ctcactgaaa aagaagtaaa gtattctgaa taatcatgtg tatgctgcaa
    13861 taagtccatc aattcagtga tttcagaaga agtttttgga aaatatgcct gttgtttgtt
    13921 tcgtaatatg aataaagtta acagcaagta gatatttttt tcttgttttg aaattttttc
    13981 tatttgctca tctgtactgt tcatttttag tgcatttaat tcaagtgagg aaaatggcca
    14041 atcgttacct ccaaatgtat ctgaaagaaa aaagtaaaga tagagacgga tagataactc
    14101 atttccttca agagtacagc aattttttac agataggatc cgtaaatgga atttttttaa
    14161 ggaatgattg agctgttttg tcagtttttc gagataggag ggcgatatat aaagttgttc
    14221 aagtaaatat ttttttggta cagcagtttg tggataattg attagtaaca tcaaatagtt
    14281 aaaacgattg gattctttta ggtagcataa ttttaactta gaaaaaacag ctaacttttt
    14341 agtttgatcg gt
//
LOCUS       DAXWLF010000062        14310 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXWLF010000062 DAXWLF010000000
VERSION     DAXWLF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.062"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(82..777)
                     /locus_tag="ACGE4U_001400"
     CDS             complement(82..777)
                     /locus_tag="ACGE4U_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFF8393573.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(867..1967)
                     /gene="ychF"
                     /locus_tag="ACGE4U_001401"
     CDS             complement(867..1967)
                     /gene="ychF"
                     /locus_tag="ACGE4U_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFF8393574.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(1976..2167)
                     /locus_tag="ACGE4U_001402"
     CDS             complement(1976..2167)
                     /locus_tag="ACGE4U_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFF8393575.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2188..3078)
                     /locus_tag="ACGE4U_001403"
     CDS             complement(2188..3078)
                     /locus_tag="ACGE4U_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFF8393576.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3065..3832)
                     /locus_tag="ACGE4U_001404"
     CDS             complement(3065..3832)
                     /locus_tag="ACGE4U_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFF8393577.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="ACGE4U_001405"
     CDS             complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="ACGE4U_001405"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFF8393578.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="ACGE4U_001406"
     CDS             complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="ACGE4U_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFF8393579.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="ACGE4U_001407"
     CDS             complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="ACGE4U_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFF8393580.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLNTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8403..8786
                     /locus_tag="ACGE4U_001408"
     CDS             8403..8786
                     /locus_tag="ACGE4U_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393581.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(8954..9712)
                     /gene="jag"
                     /locus_tag="ACGE4U_001409"
     CDS             complement(8954..9712)
                     /gene="jag"
                     /locus_tag="ACGE4U_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFF8393582.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9730..10548)
                     /locus_tag="ACGE4U_001410"
     CDS             complement(9730..10548)
                     /locus_tag="ACGE4U_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFF8393583.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="ACGE4U_001411"
     CDS             complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="ACGE4U_001411"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFF8393584.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="ACGE4U_001412"
     CDS             complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="ACGE4U_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFF8393585.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11676..13010
                     /gene="dnaA"
                     /locus_tag="ACGE4U_001413"
     CDS             11676..13010
                     /gene="dnaA"
                     /locus_tag="ACGE4U_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFF8393586.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13208..>14310
                     /gene="dnaN"
                     /locus_tag="ACGE4U_001414"
     CDS             13208..>14310
                     /gene="dnaN"
                     /locus_tag="ACGE4U_001414"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFF8393587.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFI"
BASE COUNT         3956 a         3025 c         2567 g         4762 t
ORIGIN      
        1 tattatttaa ctatggataa acaaaatgag gttctttact acccctagat atctagcaaa
       61 atagctgatc tttttgctag tttatcttct tgggacggat aaacttccaa taatcccgta
      121 ttttttcttc aagtagtaac ggattgctaa agcagttgcc ccgataatga taactactac
      181 tggatctaat actggattga taactggtgg caatagtgtt gtcgcagaaa acagtacgac
      241 ccaaagaagt gtcgttccac ctaggataag caatgtctta cccattcctg gtttcttgct
      301 tcgatctgct ccgggatatt catattgata gatgtatttg tacatcatat aaaagaccca
      361 gccaccgatt gcagaaccta gaacgagtgt agaaaagcca taagctggcg ttgttccacg
      421 tgaaaacaat ccggtaatcg caatcatgat acttaataga ccaagcaaca gcaacgtatt
      481 atctaaccac atcaataaag gggtggatga attttttact tctgctggct tattgatgat
      541 tgcttccgtt ctttcagaga ctgtcccaaa caattgacgg gctgttcttc cgcctttttg
      601 ttctgcaact aaagtaggca gaatgtcatg aagggctttt actttttctt cttcagacaa
      661 attcgctgct tctaaagatt ttttcaaatc aaaaatgtac tgttgatttc ttttggttaa
      721 ttttgtttct aaactattgt tttcttttac gattgctcgt aattgttctg gttccattat
      781 ctaatagtcc tccttagccg ttccttttct caacggtcgt cacaaaagac attctaccat
      841 attctacagc tagtttgata gcccttttac acattaaagc ggaataacat tacgtcgccg
      901 tcttgtacga cataatcttt tccttctaat cgaacacgtc cagcttcttt tgctgcatgc
      961 atgtttccgt aatgatccaa gtcttcaaaa gagacagttt ctgcacggat aaatccgcgt
     1021 tcgaaatctg aatggatgat tcctgctgct tgtggtgctt tgattccttt tttgaatgtc
     1081 caagcacgta cttcttgttc cccagcagta aagtaagtag ctaagcctaa caaatcataa
     1141 gcagcacgga tcaactgatc caaacctgat tcttcgattc ctaaagcttc taaaaattcc
     1201 gctttgtctt cttcatctaa ttctgcgatt tcttcttctg cacgagcact gaccacgatc
     1261 acttctgcat tttcttctgc agcaaagttg cgcacttctt gtacataatg attcgcgtct
     1321 gcatctgcta cgtcatcttc tgagacattg gctacataaa gaattggttt tgtcgtcaat
     1381 aaaaatagtg atttaacgat tttttgttct tcttcagtaa attcgatcgt tcgggcagat
     1441 tttccttctt ccagtacagg tttgatttta tccagtacag ctagttctgc cactgcatct
     1501 ttatctttgg ttttagcaac ttttgctaca cgagtatagc gtttgttgat agattctaag
     1561 tctgctaaca ctagttccaa gttgatcgta tcaatatctt ctagtggatc aacacgtcct
     1621 tctacgtgag taatattatc atcatcaaaa cagcgaacaa cgtgacaaat agcgtctact
     1681 tggcggatat ggcttaagaa ttgatttccc aaaccttctc ctttacttgc gccttttacg
     1741 attccagcaa tatctgtaaa ttcaaaagtc gtcggaacag tttttttcgg atgtacaagt
     1801 tctgttaagc gttgcaaacg ccagtcaggt acttctacca tccctacgtt ggggtcgatc
     1861 gtcgcaaagg gatagtttgc tgcttctgct cctgcttttg tgatcgcatt aaacaaagtt
     1921 gattttccta cgttgggcaa cccaacaatt ccagcagtca atgccataaa tttattcact
     1981 ctttctctgc atttttttca attacttttt ttaatttctt ttcaaaatct cgacgggtca
     2041 tcatgacgat atgtccgcaa ttcatacatc tgattttgat atctgcgccc attcgaatga
     2101 cttcccagcg attcgtctgg caagcgtgag gttttttcat ttcaacgata tcgtgcaagt
     2161 cgtacataaa aatagcctcc tttttgttta ttcttcatca aattgaatat tcaagatatc
     2221 aagaattcga gtcaaatctt tttgagaaag atattcaatc tcgatcttac ctttcccatt
     2281 tttttcttgg atagctacac ttgtaccaaa tttatccatc aaacgatctt cgctttctcg
     2341 gatataatat ggtttttcct ttgctagcct tgggattttt ttcttttctt ttccatcatt
     2401 ttgatttaac tcagaaacaa gctgttccag ttggcggaca gttaaatttt cagttactgc
     2461 acgattggct aatttcaaga tcaatgtttt attttttaaa cctaacagcg tacgggcttg
     2521 tcccatagat aaacgctgat cttgtaccat ttccttgaca agatccggta aagaaagcaa
     2581 gcgcaaataa ttggcgatat aaggtctgct ctttcctaaa cgactagcta cttctgcttg
     2641 tgttagtttc aggtttttca tcagcatctc atatgcttct gcttcttcta acggattcag
     2701 atcttctcgc tgcaagtttt ccagcactgc tacttgcatc atcgcttctt cgtcaaagtc
     2761 tctaacgata gcagggatcg tttctttttc agctagtttg gacgctcgaa aacgtcgctc
     2821 ccctgcaata atctcatatc cttttactgc ggaacggcgt acgatgatgg gttgaaagac
     2881 acctgattgt tcaatagaat tagctagttc ctgcaaagat acttcttcaa atgttttacg
     2941 gggctgatac ggatttggtc gcagctcatt caatggtagc tggacaacgg tatcatcttt
     3001 tacatccacc gtctctaagt tttctaaatc ttgaaacagt gcatcgattc ctcggcctaa
     3061 tcctttactt ttcttcacga cttaccactt cctttgccaa tgcttggtag acttcagctc
     3121 ctcgagaacg tggatcgtag tcgatgatcg gttttccatg actaggtgct tctgataaac
     3181 gaatatttct tggaatgatc gtttcgtaaa ctttttcgcg gaagtattta cgtacttctt
     3241 ctacgacttc atttcccaga tttgttcgtg catcatacat tgtcagtaag actccctcga
     3301 tctctaattc tggattaaag tgtttttgga ctaaacgaac cgtattcagt aattggctta
     3361 atccttccaa tgcgtaatac tcacactgaa caggtatcaa aatcgaatca cttgctgtaa
     3421 atgaattgat tgtcagatgg ccaagagaag gcgggcaatc gatcaaaata taatcatact
     3481 gactgcttac ttctgctaaa gatcctttta atcttgattc tcttgccatc attgacgtca
     3541 attcgatctc tgctcctgct aattggagag tcgcaggcac aatactcaaa ttttcgtgtt
     3601 cagtgatcaa agttgcctca tcaattggca attcattgac tagaacatca tagatgtctc
     3661 ttgtcacatc aggttttctg atcccgacac cactggtagc atttccttgt gcatccatat
     3721 ccactagcaa tacttttttg ccaaggctag caagacaagc acctagattc accgtcgtcg
     3781 ttgttttgcc gacgccacct ttttggttcg caacagatat tatttgtgcc atctcttctc
     3841 ctcctatttg attggctgct tattaggcag tccgggcttt cttgggtatt tctttggggt
     3901 tggtttcttt ttttgaataa cgatgatatg ccgttgatca gagacattcg gcaaggtaac
     3961 cgcctcttct ttgaggaatt taccacctaa taaagcaatc gcttgttttg cttctgtcaa
     4021 ttcctcttca ctttttgctg ctttcaatgc ataaaaatag ccttcttgtt tagccaaggg
     4081 caaacacaat tccgctaaca cattcaaacg agcaaccgca cgagcagtga caaggtcaaa
     4141 tgatgcacga aagtgaggat tttgcccaaa tgtttctgcc cggtcatgat ataaggaagc
     4201 atcgattcct aataatcggg ttaactcaga taagaaggtt attcgtttat tcagagaatc
     4261 aacgatcgtt atctgcagtt ctggaaaggc aatttttaaa ggtatgctag gaaaccctgc
     4321 ccccgaacca acatcgcata aagataaagg ttgcgtaaag tctacttcaa atgctaatgt
     4381 aagtgaatcg taaaaatgct tcagatatac gtctttttca tccgtaatag ctgtaaggtt
     4441 catcttttcg ttccattcga ctaatgtccg ataataaata gcgaattgat tcatttgatg
     4501 atcggtaagc tctatccctt tttctgccaa ggattggcga aattcttctg gtatcataaa
     4561 tattcctttc attgtgaaac agcaattgtt tcacagattc tctccctact ttaacgcaaa
     4621 atctctagac atgcaatcct tccatatgga ttcttaaaaa tttgaaaagt aagaaaagcc
     4681 gatagacttt ttctttctag tatgactcat aaaatataca cagaagagag aactagcttt
     4741 gagcaatttc tgcaaaaact agttctctct tctatttatt attacgtatt acgctggttc
     4801 actgactttc gcaatcttcc cttgttcgat atagaccatt aggatactta ggtctgcagg
     4861 gtttacgcca ctgattcggc tagcttgggc aattgtttct ggttggattt tctgcaattt
     4921 ttgttttgct tctgttgcta atccgttgat tgctgcgtag tcgatgtttt ctggaatctt
     4981 tttcgcttcc atgcgtttta atttttctac tttttccatg gcttttttga tgtagccttc
     5041 atatttgatt tggatttcta cttgttcgat ttcttttcgt gtcaattctt cattttctgg
     5101 aataaaccgt agaagttctg cataagtaat ctcaggacgt cttaaaaagt cgcttgctag
     5161 tacaccatct tttaatggtg ccgcattaat cgttgctaaa aagtcttgga cttcttctgt
     5221 tggtttgatt cgtacattca tcaaacgttt gatttcttgt tcaactgctt cttttttctt
     5281 caaataattc ttgtattgat cttcttttac taagcctaca cggtgaccca tttctgttaa
     5341 tcgcaaatct gcattatcat gacgtaaaag cagacggtat tcagcacgtg aagtcagcaa
     5401 acgataaggt tcgttcgttc cttttgttac caaatcatcg atcatgacac cgatgtaacc
     5461 gtcacttcgt ttgatcacta aaggttcttt tccctgaact ttcaaagctg cattgatacc
     5521 ggcaatcagc ccttgtccag ccgcttcttc ataaccgctt gtaccatttg tttgtccagc
     5581 tgtataaaga ttttcaacga ctttagtttc taatgtcgga cggagctggt gtggctctac
     5641 tacgtcatat tcgatagcat atcctgtacg catcatttct actttttcta atccagcaat
     5701 agaatgaagc atgttgactt gaacatcctc tggtaaagaa gtagataaac cttgaacata
     5761 gacttcttcc gtatttaagc cttctggttc caaaaataat tgatgacgtg gtttgtctgc
     5821 aaaacgcacg atcttatctt cgatggatgg acaatagcga gcgcctaccc cttcaacgat
     5881 ccctgtaaac attggggcac gatgtaagtt gtctcgaata atttggtgag tcgtctcacc
     5941 tgtataggtc agccagcaag attcctgatc tagtttatag gcactatctg gtgtgctgaa
     6001 gctaaaatga ttcggctctt tgtcaccagg ctgctcttct gtaacagaat agtcgatcgt
     6061 acttgatttt actcgaggcg gtgttcctgt tttgaaccgt tcgatagcaa agccaagttc
     6121 ttttagatga ttcgctagtc caatagaagg ttgagagtta ttcggacctg atgaatattt
     6181 caattcaccg ataatgattt ctccgcgaag tgctgttccg gctgtaataa caactgcttt
     6241 ggcccggtag ctcgctcctg tggacgtcac tactccatga caaacaccat cttcgacgat
     6301 cagttcttcg acgattcctt gtcttagcgt caaatttggt tctttttcga tcgtatgttt
     6361 catttctgtt gaataagcat gtttgtctgc ttgtgctcgc aatgctcgga cagctgggcc
     6421 ttttcctgtg ttcaacatac gcatttggat ataggtttta tcgatattct tacccatttc
     6481 tccgcctaac gcatcgattt ctcgaacgac aacgccttta gctggtccgc ctacagaagg
     6541 attacatggc atgaatgcta ccatgtccaa atttattgtc agtaataatg tttttgatcc
     6601 catacgtgca gcagcaagtg cagcttctga accggcgtgt cctgcgccaa caacgatcac
     6661 gtcatacgca tctgcttcat aatgttccaa tcgtttgttc ctccttttat tttcctaaac
     6721 aaaattgact gaataattga gtgattagct catcttgtac actgtcgccg acaacttctc
     6781 ctagatagtc ccagcaacgc gtcatatcta tttgaacgag atccacaggc attcctgaat
     6841 caatacctgc aatgacttct tcaagtgaga gggaagcttt ttctaacaac gcgatatggc
     6901 gggtattaga aacataagta gcgtctcttt ctcctgtttc tccgctgaag aataaatcag
     6961 aaatagctga ttccaaacga tctagtccgt cgtttttagc tacggaaatt gaaaaaactg
     7021 gttcattttc gatcaatttc ttcaattttt cttgttccag ctgtgctggc aaatctgttt
     7081 tatttagaag aatgatccgt ttcaatccac ttgtcgcttc caataattgt tcatcttctg
     7141 ctgtcagtgg ttcgctttgg tttagaacaa gtaagatcaa gtctgcttct gctaatgctt
     7201 ttcggcttct ttcgaccccg atccgttcca ccacatcttc tgtttcccga attccagctg
     7261 tatcgattag cttgagcggt accccccgaa cattgacata ctcttcgatt acatctcgag
     7321 tcgttcctgc aatatccgta acgattgctt tttcttctct taacaaatgg tttaacaaac
     7381 ttgatttgcc gacatttggc cggccgatga ttgccgtatt taacccttcc cgcagaacct
     7441 tcccttgctt ggatgtagca agtagcgctt ggattctttg ttggatcatc tgcgcttttt
     7501 caagcaataa cttcgtagtc aattcttcga catcatcata ttctggataa tcaatattta
     7561 cttctacttg tgctaatgtc tctaaaatct cttgtctcaa tgagcggatc aaagcagaca
     7621 gattgccgtc taactgatta agagctagtc ccattgcttt gtctgttttt gcccgaatca
     7681 aatccatcac agcttctgcc tgtgaaaggt caacacgacc attcaagaat gctcgttttg
     7741 tgaattcgcc tggctcagct aatcttgcac cttctcttaa caatagctgc aaaatttgat
     7801 tgactacaac gatcccaccg tgacaattga tttctactac atcttcccgc gtaaatgtct
     7861 ttggtgctct catcacggag accatgactt catcgacaag ctgattgctc ttgggatcaa
     7921 cgatatgtcc ataatggatc gtatgacttg gtacttctga taatcgtttg tttccagatt
     7981 gataaacttt atctgctagt tgcacagctt gatcgccact taaacgaacg atactgattg
     8041 caccttctcc aggtggggta gaaattgccg caatcgtatc aaattctaaa gtaatttcag
     8101 ccattttttc acttcctttt tcattttgcg cacaaaaaaa gtgcccactc caccctttat
     8161 gcacaataaa cggtgaactt cgcactttga ctgcttgttc ttgattaaat tctggtcata
     8221 gattatcaca cttgctataa ggaatcaagt gtttaaaaca attagatgaa gttgagcctg
     8281 ttctgcttcc attaccttat atttaattgt ccttggatgt ctctttgcgt tctttggttt
     8341 attcgaagag aagatactgt ataaacaagg ctttatcttt cttaaaaaaa acggtacact
     8401 aaatggtagc gctatctaaa ctatacactt ttgaaaggag aaaatatgtg agcccgttca
     8461 gatctgctgc attggaagaa gaaagtgacc ctcggatcaa cgatgtcgtt ttatctacag
     8521 ggaatatcaa ccagaaatat atcgtccgcg atatcgtttt tgttgtggaa tgcttagaag
     8581 ctgatttgtt tgacccatct tttcccaaag atgacctttt cttaacaacg aagaggaaat
     8641 taaagacgaa ggtacttgaa tatggtgcga atgcagtaat caactgtcat tttgagtatc
     8701 agcaaaaaaa acgcgacgga aagtattttc tggaactttt tgcttatgga acagtgatcc
     8761 aatttatcca aacaacgatt ggttaaatga aacagacaaa aaaagcggat caaaactgaa
     8821 gacagtttcg atccgctttt ttctatcaaa gttagtgact acttcatcgt atactctgat
     8881 aagaatgatt tctagtaaaa tatacttggt ttgaaaaact ttgtcaacgt gatttattca
     8941 aaaagtaatg gaatcaataa tattttttag caggttctac tactaaataa cgataaggtt
     9001 cctcaccttc cgaatgtgtt tttacatgtt catctttgct taatgcagca tggatttgtt
     9061 tacgttcaaa tgcaggcatt ggttctaaga aaacaggctg ccctgtccgt tttacttttt
     9121 cagccgtgcg atcagctaaa cgctgaatga tctcttggcg cttttcacgg taattgccta
     9181 cattgactac tacagacagc ttatttcttg ctaaacgatg gatgaagact tgtgccagat
     9241 attgcaatgc attcagtgtt tttccatgtt tcccaatcag tatgccttgc ttttgtgttt
     9301 ctaaatggaa aacaaccata ttatttgatt ctctttccat tttgacaaga gctggagcat
     9361 caagctgttt tgaaatttca gtaagataaa ctgccaattc cgttaaagct gcttcatcac
     9421 ctagctcact tacttcttct gttttttctt caagaatcgt ttcgttgact acctcgatct
     9481 cagctgttgg ttcttcttcc aaaatttctt ctacagtctg ttctactgtt tcagaaactt
     9541 tttcgctgat acttggctgg atcgaaagcc gtgcattctt tcgacccatt cctaggaaac
     9601 ctttttttcc ttcttccagt atttcgattt cgacttggtc tttggccaaa cccaaatcag
     9661 ccagacctag ttggacagct tcgtctattg ttgcagcttc ataaatcggc attagcatta
     9721 cctcccctat tatttccgtt ttttcttagg attcattgct ttcttaagag cacgttctct
     9781 ttcgcggatt ctccgtgctt cttcttcacg ctcttttcga attttgaaag ggttgttgat
     9841 cagcagcgtt tgtacaactt ggaaggcatt ggaaactacc cagtataatg aaagaccgct
     9901 ggctaaattg acacccatca ctaaaatcat tacaggcatc acgaaattca tgatcttcat
     9961 tgatgcattc gattcaattt gactcatgct agataaatac gtacttgcaa aagtaaagat
    10021 tgctgctaaa atcggcaaaa taaatgttgg atccgcgttc cctaagttca accataagaa
    10081 atgcccttgt ttcaactctg gtacacgtga aatagattgc cataacgcca tcaagattgg
    10141 catttgtact aataaaggaa gacaaccagc atatggattg acgccatttt ctgcatataa
    10201 acgctgctgc tcttctctta acttactttg tgtttccgga tctcttgaac tatattgttg
    10261 ctgcagtgct tttaatttcg gttgtaattc ttgcgtttta cgcatgctct ttgtttggaa
    10321 atgcatcaac ggcaatagga tcacgcggat gatcagtgta aataaaatga tccctatccc
    10381 tgcattacca aatgacaacg ccttgatcgc ctcagcaaag taataaacaa tataacgatc
    10441 ccaaattcct gtactctgtg cgctgacttc cccagttccg caaccggata agacgatgac
    10501 caaactaacg ataccggcca tcaaaagtaa acgtttgtat ttcttcactt actcttttcc
    10561 tctctgtcga ttatttttgc aagttttaag acgtggacta aattggagcg aacctcatca
    10621 taagtcaacc ccttcacact tggtctagca atcacaataa aatccaactc cggatcaatt
    10681 tcttctttta tagaataaac tgctgctcgg atttttcgtt tgacttcatt gcggtttacc
    10741 gcgttgccga ccttttttcc tacagataaa ccaactcgaa aatgaggctg tcccggtttt
    10801 tccaaacggt aaacgacgaa tttgcggtta gcaaaagaat ctcccgtatg gaacacggct
    10861 tgaaactctc gttctttttt tactcgatac gcttttctca ttagttttct ttccttttct
    10921 atctctcact tacgctcata atcataccat aattacgatt gtcctttcat catttcccta
    10981 aaaaagagta taaaaaaaac cactgaaacc ccagtggcct aagctgaaag aacttttctt
    11041 cctttgcgac gacggctagc taacacacga cgtccatttt tagtactcat acgtttacgg
    11101 aatccgtgaa ctttttgacg tttacgttta tttggttggt atgttctttt catatattcc
    11161 acctccaaaa tgttcttatc tattttataa acacagacat actttgatag tataacgaca
    11221 aagtagtgat tttgtcaata taaaatagaa gatcgattgt taaaacttat ccacaagatt
    11281 atttttttct atgggaatcc tatgtggaaa aagttatgca cagggattat ccacagataa
    11341 taaaaggctg gttttacaca gaaaacacac gtgtaaaaaa agtaatccac aatcaggcga
    11401 tttcctgtgt attccgtctc ctaactattg gtatatctgt attttttatc cacagacgca
    11461 agaggaaatc tattggtttt tacgagtttt ccacctgtgc attagtggtg tggaaaactt
    11521 tttttgacaa ctgtgttttt ctcttttttc tttttgtcga ttgtgtggat aattgtaatt
    11581 aaaaaatttt tttcttcggt ttctgtggaa aactattgaa aaaaatgcta aattagtact
    11641 agttattcac tttttaatga aggaggaggc cttatatggt atccctcgat gctttatgga
    11701 atgaattaaa agcaacatac caaaaggatt tatcgcctgc tagctataat acatggatcg
    11761 aaacagcgaa ccctcgaacc cttgatcaaa accagctcgt tgttgaggtt cctagcaaga
    11821 ttcataaaga atattgggaa aaaaacctgg cgacaaaaat cgttgaagtc ggttatatgt
    11881 tatcaggaaa tgagattatc cctcgcttta ttactggcga agaagcagag caagaagaag
    11941 ttatagaaga aaaaaatcca aaagttgtgg cacctagtcc gctaaaaaaa gccatgctga
    12001 accctaaata tacctttgat acgtttgtca ttggtaaagg aaaccagatg gctcatgcag
    12061 cagcgctcgt tgtagcagaa gatccaggtt ctatttataa cccgctcttc ttttatggag
    12121 gcgtgggact tggtaagact cacttgatgc atgcaattgg tcatcaaatg ctgcaaagtc
    12181 agcctaatgc caaagtaaaa tatgtaagca gtgaaacttt tgctaatgat ttcatcaatt
    12241 cgatccaaaa caaaacagca gaagagttcc gtcaagaata tcgaaatgtt gatttactgc
    12301 tagtggatga cattcaattt tttgcagaaa aagaagctac gcaagaagag tttttccata
    12361 ctttcaatgc cttgtacaat gaagggaaac aaatcgtact aaccagtgat cggttgccaa
    12421 acgagattcc gaaactgcaa gaacgccttg tttctcgctt tgcatggggg ctgtctgttg
    12481 atattacacc gccggatctt gaaacaagaa cagcgatttt acgtaaaaaa gcggcggctg
    12541 aacgtctaga gatcccagat gacacactaa gctacatcgc aggacaaatt gattctaaca
    12601 ttcgtgaatt agaaggtgcg cttgttcgag tgcaagcttt tgctgcgatg aatagtgaag
    12661 atatttcgac tagtctagct gcagacgcac tgaaaacgtt aaaatcagga aaaggacatc
    12721 ctcaattgtc aatcctgcag atccaagaag aagtggcaaa gtattatcac atccaattga
    12781 aagatctcaa aggaaagaaa cgcgtcaaat ctattgttgt tcctagacaa atcgctatgt
    12841 atttggcacg tgagctcaca gataattctt taccgaaaat cggagctgaa ttcggtggca
    12901 aggatcatac aacggttatc catgctcatg aaaaaatcca gcagctgatg gattctagtg
    12961 tcagtatgca aaatgaagta tcagaaatca aaaacttttt actaaattaa tttgtcgatt
    13021 ctatcaactg aaaatagcat gtggataaaa gaaaaaaacg ttcaaaagtt atacacactt
    13081 tttccacagc tgttttttcg tttctctctt acgttttttg agttttccac agaatcaaca
    13141 tgccctatta cttttattat tctttttaac taataataaa taaacaaaca ttcaaaggag
    13201 atacatcatg aaagttactt taaaccgagc tagctttatg caggaattgc aaactgttca
    13261 acgagctatt tcaagcaaaa ccacgatccc tattttgaca ggtgtaaaaa tcacactgac
    13321 acaagaaggt ttgactttga cggggagcaa cgctgatata tcaattgaaa cttttttgtc
    13381 tgttgaaaac gaaaaagcaa atatgcaaat cgaatctact ggttccattg ttttacaagc
    13441 acgtttcttt agcgaaatca ttcggagact tcctgaagaa acatttactt tagaagtttt
    13501 agaaaataaa caagtagcga tcacttctgg aaaagcgaat tttatcgtaa atggattaga
    13561 tgcagataac tatcctcatc ttcctgttgt cgaaagccat aaccagatga aattacctgt
    13621 acacgtattg actaaactaa tcaacgaaac agtttttgct gtctcacaac atgagagtcg
    13681 tccaatcttg acaggtgtgc atttcatttt atctgataat tctttattag ctgtagctac
    13741 tgattctcac cgtctaagtc aacgcgtgat tccagtagaa caagcggctg atcattttga
    13801 tattgttatt cctggaaaaa gtttgatcga attatctcgc tcattaacaa atgaagaaga
    13861 aatcgtcgaa atcagcatta tggaaaacca agtgttgttc aaaacagaaa cgatgtattt
    13921 ctattcccgt ttgctagaag gaaactatcc agataccaat cgtttgattc cttcaagttt
    13981 taatacagag gttgaatttt ctgttcctag ctttttagcg gcgatcgaac gggcttcttt
    14041 actttctcat gaaggccgta acaacatcgt tcgtttatct attcgtccag atgcggttgt
    14101 tttatacgga aattcgccag aaatcgggaa agtagaagaa agcttgagtt atacagcaag
    14161 cagtggcgat ccattagata tttctttcaa tccggattat atgaaagcag cgttgcgtgc
    14221 atttggagat atgagcatca aagtgaaatt tatctctgct attcgtccat ttacattgga
    14281 accaacggaa gatggcgttc aatttattca
//
LOCUS       DAXWLF010000063        14198 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXWLF010000063 DAXWLF010000000
VERSION     DAXWLF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.063"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(34..1194)
                     /locus_tag="ACGE4U_001415"
     CDS             complement(34..1194)
                     /locus_tag="ACGE4U_001415"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFF8393588.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
     gene            1359..2534
                     /gene="mltG"
                     /locus_tag="ACGE4U_001416"
     CDS             1359..2534
                     /gene="mltG"
                     /locus_tag="ACGE4U_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFF8393589.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            2670..3149
                     /gene="greA"
                     /locus_tag="ACGE4U_001417"
     CDS             2670..3149
                     /gene="greA"
                     /locus_tag="ACGE4U_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFF8393590.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3317..4048
                     /gene="liaF"
                     /locus_tag="ACGE4U_001418"
     CDS             3317..4048
                     /gene="liaF"
                     /locus_tag="ACGE4U_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFF8393591.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            4045..5112
                     /gene="liaS"
                     /locus_tag="ACGE4U_001419"
     CDS             4045..5112
                     /gene="liaS"
                     /locus_tag="ACGE4U_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFF8393592.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            5119..5751
                     /locus_tag="ACGE4U_001420"
     CDS             5119..5751
                     /locus_tag="ACGE4U_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8393593.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            5891..6556
                     /locus_tag="ACGE4U_001421"
     CDS             5891..6556
                     /locus_tag="ACGE4U_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFF8393594.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            6584..6877
                     /locus_tag="ACGE4U_001422"
     CDS             6584..6877
                     /locus_tag="ACGE4U_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFF8393595.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(6945..7892)
                     /locus_tag="ACGE4U_001423"
     CDS             complement(6945..7892)
                     /locus_tag="ACGE4U_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFF8393596.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8070..8873)
                     /locus_tag="ACGE4U_001424"
     CDS             complement(8070..8873)
                     /locus_tag="ACGE4U_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFF8393597.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9019..9933
                     /locus_tag="ACGE4U_001425"
     CDS             9019..9933
                     /locus_tag="ACGE4U_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8393598.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            9981..10451
                     /locus_tag="ACGE4U_001426"
     CDS             9981..10451
                     /locus_tag="ACGE4U_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFF8393599.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            10536..11144
                     /locus_tag="ACGE4U_001427"
     CDS             10536..11144
                     /locus_tag="ACGE4U_001427"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFF8393600.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11278..11478
                     /locus_tag="ACGE4U_001428"
     CDS             11278..11478
                     /locus_tag="ACGE4U_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8393601.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            12232..13458
                     /locus_tag="ACGE4U_001429"
     CDS             12232..13458
                     /locus_tag="ACGE4U_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8393602.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13566..14036)
                     /locus_tag="ACGE4U_001430"
     CDS             complement(13566..14036)
                     /locus_tag="ACGE4U_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFF8393603.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         4836 a         2180 c         2888 g         4294 t
ORIGIN      
        1 atacagagat gacaactaat attatgtcaa aatttaattt gataagtgaa caccttccat
       61 cagttcataa atctgatttc ttaaatcagt ggccggcaat ttgatgttct tatccgattg
      121 gatattactt tccatgacga ttgcatcttt agtgtctttt ttcatcgcca ctactgcatg
      181 gaatcgattg atattcccct gagcaaggat ataatcgttt ccactgtaaa caccgccgct
      241 gtaaggatgt ttatcaccat tattttgcca caatgagaaa agtagttctt ttggtatgat
      301 accgccagaa agaacactgg taaagaattt gttcaagtca taaacagaca tacttatgtt
      361 tcctgtacct aattcatttc ggatgtcggt ttcaggctcg atcaatgctt ttgaataatc
      421 gtcagttgca gacatctggt aagatacagc atgttgaggc gaatttttta cattgtcata
      481 aaaatctgta tgttctagat ggagtggacg aatgatttct ttttttagaa gagtttcata
      541 aggctgattc gttagtttga tcaatatccc tgtaagcaat gtataattca atggttcata
      601 atgatacgta tcataatcag taagtttcaa atgatgaagg gcgaactgaa tgatttcact
      661 atccgacata ggtttgtcgg gagaagcagt tcgatgaaga ccagagcgca tataaagcat
      721 atttttgatt gtgatttgct gacttccagg aatctgagga taaaattctg ctaactttgt
      781 gtcaaaagtt aacttaccag cttggatttg ttttagtatc agtaaagcag tcattgcttt
      841 ttgaatcgaa ccgatttgat agtaaataga aggtgaattc gattctttta cgcgcagatt
      901 tgcatatcca tatccttttt ctaatagtac gcgattattt ttgcgaacat aaatcgaacc
      961 gatataatct gatttttgta gtaattgatc aattttgcta gcaagcctct gctcatcggg
     1021 cgttaaattg gaagtaagtt gtgttggctc cgctttataa gtttgggaat gaggtatttt
     1081 tgttaattga cctcctataa tgatgcctgt tgaaaagaag ctgacagaca ataataaaat
     1141 tacaagaaaa gaaatagaaa ttttttgttt ttttcgatgt tttccttttg acatgttaat
     1201 tcctttctat ccatttttaa ctatttttca ttgtaatcct caaaaagagt atttgccact
     1261 tacaattttg acataatatt tggtaaaata attcacggca gtagaaattt catgtataat
     1321 aaacagagtt acgaagatga tagtaaggag gagttgtttt gtcaggaaac aaacaacacc
     1381 caagtaaaga taaaaaagaa gccagtaagg cagctacagc agatataatc gacaagcgga
     1441 gaaatctgcg taaaaaagaa gataagattg tgaggaagat cattttagtg attgctctta
     1501 cattattaat tattggcgga tttcttggat ttaccgtgta tcgttatgta gatagcgggt
     1561 tgaagccttt agataaatca gatgaccaat tagtccaagt ggagatcccg agtggctctt
     1621 ctaacaagca gattggtgag attttggaga aggacaatat tattaaaagc gggattgttt
     1681 tcaattatta tacaaaattt aaaaatttaa ctggctttca agctggctat tatcagttag
     1741 cgcctaatat gactcttgac gagattggca aacagctgca agaaggcggc acttctgagc
     1801 caacaaaagt tgcagatggg aaaattgcca ttccagaagg ctacgacatc gatcagattg
     1861 ctgagcgggt agcgaaggta actggtaaag acaagaaaga atttttagat ttagtaaatg
     1921 atgagacttt ctttaacgag ttgtatcaaa aattcccaga attgttggaa agtgcctcta
     1981 aagctgagaa cacaaaatac cgtttggaag gctacttatt cccagctact tatgattact
     2041 acacgagtac ttcgcttaag gatcttgtga ttgagatggt gaacaaaaca aatacggtca
     2101 tgcaaaacta ttattcagct atcaaacaga aaaatttgac tgtacaagaa gttttaactt
     2161 tggcttctct agttgaaaaa gaaggcgtaa aagaaaatga tcgtaaaaat attgcacaag
     2221 tcttctttaa ccgaatcaag gcaaatatgc cacttcagtc agatatctct gttttatatg
     2281 cattaggaga acataaagaa ttggtgacgt atgaagatac agctgtagat tcaccataca
     2341 atttgtatac caataccggc tatgggcctg gtccttttga caatccaagc gaagaggcaa
     2401 tcaaggcagt attagaacca gcagaaaatg actattatta tttcgtagca gatacgagca
     2461 ctggaaatgt ttatttcgct aaaacgtatg aagaacatat ggaactagtg caaaaatatg
     2521 taaataattc ttagaaaaac aggggctagt cattgatcat ttttagaaaa acatttacat
     2581 tttaagaaaa gcgtggtaat cttttttgga ctatattcct caaaagatta ccttttgcgg
     2641 tattttatcg aaaaaaggag acgttacaca tggtagagaa agtttatcca atgactctag
     2701 aaggtaaaga aaaattagaa aaagaattag aagaactaaa aacagttaaa cgcggagaaa
     2761 tcattgaacg aatcaaaatc gctcgcggtt tcggagatct ttccgaaaac tccgaatacg
     2821 aatcagctaa agatgaacaa gcatttgttg aaggaagaat cacaacaatt gaaaatatga
     2881 tccgttttgc acaaatcatc gataatgacg gcgtagatgc agatgaggta tcgatcggta
     2941 aaacagttac gttcatcgag ttgcctgatg gagatgaaga agagtacacg attgtcggaa
     3001 gtgctgaagc agacccgttt tctggaaaga tttccaatga ttctcctatc gcgcaagcat
     3061 tgattggcaa acgtaaaaat gatgaagtaa ccattgctac accaggtggc gatatgttag
     3121 ttaaaatcgt caaagtagaa caagcgtagt ctgactgaaa gatctagaga gttcatctta
     3181 ctgatgacgc actctagatc ttttctcttt gagtattgca ggtttcactg aacgatctta
     3241 catctaaagt cctatagcca acagtcaaaa aaataggtat gatgaaatga gaatgtgtca
     3301 ggagggagaa aaagcgatga atagttcttg gcgttttttt gtggtagtcg aagcactgct
     3361 gctgttattt gctgtttggc aaatcgtaaa caatactgga cttttgcttt tagtaatatt
     3421 cggtattttc aatatatatc tagctatgcg caaatatcct cgtactaaat ttcaaaattt
     3481 tcaattgatc ttagggagct tggtcatttt ttttagctta gtcaacagtc ctgctttatg
     3541 gatgatggtt gtattggccg ttttatttat cgggctgaaa ggtgtagaga tttcaggaat
     3601 agatttgaca aaaaatgctt tttggcgaaa aaaacagatc atgatggtcc aaacagagca
     3661 attaaaaaca cataataatg agcgtaaaag acagcaatta tttggtaacc aacgtattgg
     3721 aaacgacgtc tatgaatggg atgatatcaa cattgccatc atttctggtg atactatcat
     3781 cgatttgggc aatacacttt tgccaaaaga tgataatatt gtgattgtaa gaaaagggat
     3841 aggcagaaca cggatcttag tacccctggg agtggctatt cgtttagagc atgcgacatt
     3901 agtaggaaat gtcttgtttg aagaagagca gttttcctta aaaaacgaac agatcaaaat
     3961 ctgcagcagt gattatgatg aaaatcctcg cagattgaag atcattacca atactttggt
     4021 tggagatgtc gaggtgatac gaatatgatg ggaagaatat ccaaagcaat gctagctgtt
     4081 tattcgggga ttgcggcttt ccttattatt ctattttcac ttttcactta tttttatgcc
     4141 agtaaccaaa gtcattggtg gggagaattg ctgcgtgcgc gtttattata cgttccgctt
     4201 atcttccatt tgatggcaat ttcattaggt gtaggattga tcgtatttct attgttatca
     4261 cttattcaaa aggcaaaata cgggaaaatc gaagaaaagc ttcgtgcact ttcttctggc
     4321 aattatgaat ccaaactttt gcatcagccg atcccaagcg cgtcggatga tctgtacatc
     4381 aaagatattg atatggaaat tactaaaata aaagaaaaaa tgatcgaagt atccagtgaa
     4441 ttacagatta tgactagccg tcctcaatat gtagatggac aaaccaaaga agagatttta
     4501 aaattagaaa ggcatcgatt agctcgtgag cttcatgatt cagtttcaca gcaattattt
     4561 gcagcgatga tgatgatgtc ggcattgacg gagcaagcag aaaaaagtga gacaccagaa
     4621 atgttccgta agcagttgaa aatggtggca gagatcatca acgcttccca gtctgagatg
     4681 cgtgcgctgc ttcttcatct gcgtccagtc aatttagaag aaaaaagttt aaaacaaggc
     4741 attgagcagt tattgaagga attgcagaat aaaatccaga tttcgctaaa atgggaggta
     4801 gaagatgtaa aattaactag ttccattgag gatcacttgt tccgaatcgt tcaagaattg
     4861 ttatcaaaca cattaagaca tgctaaggct aatgaattag aagtctattt gcataaaata
     4921 gataacaatc ttttattacg tatcattgac gacgggacag gatttaatat gaatgaaacc
     4981 aaaacgggaa gttatggatt gaacaatatc aaagaacgag tagctggtat tggtggaaca
     5041 gtaaaaatca ttagttttaa agggcaaggt acgagcgtgg agaccaaggt ccctttgatg
     5101 aaggaggcat agataaagat gataaaagtt ttattagtag atgaccatga aatggtacgc
     5161 ttaggcgtct cttcttattt atctatccaa gaagacattg aagtgattgg agaagctgaa
     5221 aatggacgtc aaggctatga aaaggcgatg gcacttcgac cggatgtcat tttaatggat
     5281 ctagtcatgg aagaaatgga tggcatcgaa tcaacaaaag caatcttaaa agattggcca
     5341 aaagccaaaa tcattatcgt cacaagtttt attgatgatg aaaaagttta tcctgcaatt
     5401 gaagcaggag cagcaggata tttgttgaag acgtctacag cacatgaaat agctgatgct
     5461 ataagggcta cgcaacgcgg cgagcgagta ctagaacctg aagtgacaac aaaaatgatg
     5521 gaaaaaatga gtcggcgaaa tgatccagta ttgcatgaag aattaacgaa tcgagaaaat
     5581 gaaattttga tgctgatttc tgaagggaaa agtaatcagg aaatcgcgga tgaattgttt
     5641 atcacactga aaacagtcaa aacgcatgtg tcgaatatat tagctaagtt agaggtagaa
     5701 gaccgaactc aagcagctat ctatgcgttt aaacatggtt tggtgaaata aaaacgatga
     5761 taaagaggat aatcaaaagc tgagaaaata actattattt ttcttggctt ttttacaata
     5821 atgatgcttg cttttgtttt tggaaaggct ttgttatact gagaaaagaa gatactaaag
     5881 gaggaaaatg gtgaaacaaa attttgcaat tgtaggcctt ggacgatttg gcggaagcat
     5941 ctgccgtaca ctgatcgaag ctggacaaga agtactggct attgacagta atgaagatcg
     6001 agtcaatgaa tatatgaata tagccaccca tgctgttgtg gcaaatgcac aggatgagat
     6061 gacacttcgt tcattaggta taagaaattt cgatcatgta attgtcgcta ttggagaaga
     6121 tatccaagcc agcatcttag ttactctgat ggtaaaggaa atgggcgtac cgaatatttt
     6181 agcaaaagcc caaaatgagt accatgcccg tgtactagag aagatcggag cagatcgtgt
     6241 cgtacaccct gagcgagaca tggggatacg gattgcacat aacttagtat caaagaatat
     6301 tctagactac ttagagctgt cagataaata ttctttagca gaaatccgag tctccaatcc
     6361 taaatttttc aacaaaacat tagctgaatt aaatttcagg caacgttttg acttaacagt
     6421 agtcgccata cgaagagtag atcaaacggt tatcgcttct cctgcagcag atgaatatgt
     6481 tcgtgaaaat gataatctac tggttattgg agagacaggc gatgtggata ttctagacga
     6541 taagatgaat caatagaaag aaaagagaaa gtaggttgtt gggatgaaat taacggtaac
     6601 gccaaaagca caaaaatggt ttgactcaga aattgatctg ccagaaactt acggaatacg
     6661 tttttttgga aaagtctacg gcaaaacaga ggttcatgat ggtttttcga ttggaatgtc
     6721 agttgaacag ccagaacatc ctgtaaagaa agtggaaatg gatgggacac ttttttttat
     6781 cgaggaagca gatgaatggt tctttaaagg atatgattta cttgttgatt atgatcccaa
     6841 cctggaggag ccaacctatc actttaaaaa acaataaaaa aagagagggt gacacaattt
     6901 ttgtcacctt ctcttttttc ttttgaaaat attgttttca gctattaata tcctgcagac
     6961 aattttactt gattggtttg aagctcatgg gtggcaaagt agcttttcag attggttaaa
     7021 aaaatagcca ttaatttctt ttgaaaatca ggtgtcaaac ctgcaatgtg ggaagtaatc
     7081 agtacattgt ccattttcca aagaggatga tcttgtggaa gaggctcttc ctcaaaaacg
     7141 tctaatgctg caaaggaaag ttgctggtta tttaaagcct gcaccagatc ttgtgtctta
     7201 acagaagcac cacggccaac attgatgaat acagattttg gatcaaagtg ttcaaacata
     7261 tgctgatcaa agagcccagt tgtttcctcc gttagaggta agatattgac tacgaaatct
     7321 acttcaggaa gaacttcctt tagttgattt aaagaaaaag tcttttccat tccttctgct
     7381 ggatgaccgg atgtattgat tccatagcag ttcatgtcta atgctttgca tttttttgca
     7441 atcatttttc caatatggcc agttccaatg attagtattt ttttcccttg gatagattgg
     7501 taatggaccg atgaacctaa ccatttcatg tcactctgtg ccttttgtgc ttgaaaaaga
     7561 ccacgagaat acccaaggat gattcctaag acatgctccg taatcgattc actgtggatg
     7621 ccactaccat ttgaaagtaa aatatgtttc tttgaaaaag tctcaagggg tagctgatca
     7681 acaccagctg agatagattg gacccatttc aaggtagaag aggctaatag tttttcttgg
     7741 tagtctgagt tccagcccaa actgatctct acattttgta gatcctctgg ttgtaattct
     7801 gttttaaaga tataaccagg cgctatagta tggatttctt cgataaattc atcacgaaat
     7861 ggacgatctg cataaatgat tctttctgac atagttaatt tcccgccttt ctttactcta
     7921 agtgtagcgc atacaagaag aactttgaag cgccttgctt gttttttgag aaagaaatat
     7981 ttttcaaata aaaaaggggc tgtgacaaaa aaatgtcaca acctctaaaa gggtctattt
     8041 aagtagaaat aacgagttgt ctttatttct taattgatcg atatttttgc ccatcctttg
     8101 acagcttgag caaaaataaa gattcctgca aaagcaatga aggcgctagt gtcaaaagaa
     8161 ggaagaaaga ccatcaagat tgttgcaatt atagtaggca atgaaattga atagatcaat
     8221 gtttttaaac aatcaagaaa agtagctttt gttagtcgaa agcgtgcata gagaaaagca
     8281 gcaatgttgg taaatagcaa agtgatcagc agattcagaa aactaggata aatagaaaat
     8341 ataaatgtca ggacttttat ccaaagtggt atagatgctt cacttaaaaa agaacggagc
     8401 tgcttacctg ttaagttttt taaagattca ttggtgtagg gaagttctaa ttggttattg
     8461 ttcaacaaag cagaagtcgt gcctgtatta ggaagagcta tgataagttt gttttttaac
     8521 atcccgacac taacaaaatt ccccatcaaa tcagtagaga tgtcctgttc tgttcgtttt
     8581 ccctctggat caaatgtgaa gataatggag tttgtttggt aaatgaatcc ttctttttct
     8641 tcggtttgaa gctgcccgtt ttggattgta aaatcaggaa tgcgagtagc aatcttttgc
     8701 ccatccaatt gaatgttatt taagacttga aaaacttgaa aagagattgg caaagccata
     8761 attatactca aaactaaaag atagacaatg gctttccaaa atgggacatt tctcgcctcc
     8821 tttaggtctt tgaatcgtat aaaggaactg ataattaatt gttttgttgt cataagtact
     8881 cctcatctag tagtgtcatc acttgcaatt ctagcgaagt atcaagtgaa atacaagtgc
     8941 tttagggaaa catctgaatg attggtgaaa tatcaaaaaa atgttattct tgtctaaaga
     9001 ctatccaata taaataaaat ggaggaagga atagtgccta cacagccaca gagatcatca
     9061 aaaaaactat ttggagtagt ggttttgaat gcggtgatca ctttatcaga atttattgga
     9121 gggattatgt cagggagttt gtctcttgtt tcagatgctt tccataacct tagtgataca
     9181 ttggcaatta ttttttctta cggggcacaa aaaatggccc gaaaagaagc aaataaaaaa
     9241 cgtacgtatg gctaccagcg tttagaaata ttgagtgctt tcatcaactc attcatattg
     9301 atcatattat ccttgttttt aaccgttgaa gcattcaagc ggttcacttc accagaaaag
     9361 ataaatagtc atttgatgct aacagtagca gttattggat tgctcgcgaa tttgttctca
     9421 gtattattat tacgacaaga agcagatgaa agtttgaata taaaatctag ttatttgcat
     9481 ttgttaagtg atacactgtc ttcggtaagt gtgattatcg gagcaatttt gatccgtttt
     9541 ttcgatatct actggatcga tccagttatt acactgatca tttcgatata tatattgata
     9601 gaagcgataa tggtgatcaa aaaagctgcc gctattttga tacaaagtgc acctgctatt
     9661 gactatgaaa aaatggaaca agaaattgaa gcaatcgatg gggtaaagaa tgttcatcat
     9721 gtccatattt ggcagtatag cgaaaagata attattttcg acggacatat tgattttgaa
     9781 gatcagctac taagcgagat tgaaaagata tatcctcgta taatttcttt attaaaatta
     9841 aaatatggta ttacacatgt gacgatccaa gcagagacaa aggcaaagga tcaaaaaaaa
     9901 ttaatttttt taaataaaga tgaaagttat tgaaatatag agtatgataa ataagaattt
     9961 ttaataaggt aggttggctc atgaagcagt atcgacagtt aaaaggatat ttagaagcaa
    10021 aaggatattg ggagatacta gtctatctct tttttggtgg tttggctacg attgtaaatt
    10081 ttgttagttt tgctctcgca cgccaatatt ttgatttaag tatggcctta tccaattcta
    10141 tttcgtggtt ttgttctgta ttgtttgctt ttgtaacgaa caaactttgg gtattccatt
    10201 caaaatcacc gaattttaca catgcactta ttgaatgcgg taagttcttc ttctaccgta
    10261 tcctttctta cggattggac atgggggcaa tggtattgtt gatcaatgtt atgaacagca
    10321 atgagtatgt ggcgaaaatc atcactcaaa ttattgttat cctagcgaac tatattttca
    10381 gcaagttgtt tatttttaaa gaaactgagg tttttgaaga agaagtaggt caaagtgaaa
    10441 aagacgacta ggcataacaa ttgaagaatt tccttctctt tgttactctt tgtaatgtag
    10501 acattataaa gaaaacattg aggaggaatt tttttatgac ttacacatta ccagatctac
    10561 cttatgcata tgacgcattg gaaccttata ttgacgaaga aacgatgcat ctgcatcatg
    10621 ataagcatca caatacttat gtgacgaatt taaatgcagc aattgagaaa tacccagaat
    10681 taggcgaaaa aacaatagaa gaattattat ctgatatgga cgctattcca acagatatca
    10741 agacagctgt acgtaacaat ggtggcggac atgctaacca ttcatttttc tgggaaatca
    10801 tggcaccaaa tgctggtggc gaacctacag gagaaataaa agaagcgatt aatgaagctt
    10861 ttggtgattt ttcttctttt aaagaagaat tcaaaaaagc agccgctgga cgatttggtt
    10921 ctggatgggc ttggcttgta atggaaaatg gaaaattagc tattacctct actgcaaatc
    10981 aagattctcc attgatggaa ggaaagacac caattctagg tttggacgtt tgggaacatg
    11041 cttattattt gaaatataaa aatgtccgac cagattatat tgcagcattc tggaatgtta
    11101 ttaattggga tgaagtaaac aaacgtttcg aagcagcaaa ataaccattt cctccgtcat
    11161 atcagtctta aaaataattt ggaaaataaa agagcgaaag cttgctagcg ttttctaaat
    11221 tgggtataat gttcttgcaa ggttacttgc aagaactttt ttggaggcta tacatttatg
    11281 gaacacggaa cggttaaatg gttcaacaat gaaaaaggat ttgggtttat tacagtagac
    11341 ggcggagatg acgtatttgt tcatttctca gcaattcaag gagacggctt caagagccta
    11401 gaagaaggtc aagaagtaga attcacgatt gttgaaggtg cacgcggacc tcaagctgct
    11461 gaagtgacta aattataata atttgttgaa taaagacacg acttcttaga gagtcgtgtc
    11521 tttttgtcta tttatgaagt ttttagattt tttggctaag taaatgcatt tgttcacttg
    11581 acatgtccta actaattatc aagcaaggaa cattttcttc gttagtaatg gattttaaag
    11641 aaatttcagt ttttggatgt tgaataaaag accatttgtg aaaccactag tatacatact
    11701 cattaccatg tcaattctat tactttaaaa gaaaatcgtt ttactttgaa gaggagcata
    11761 tctatccatt aaaagatgat ggtaatgaaa tcactagttc gagtcatttt aagagccatg
    11821 cagtgagctg gaaataaaac gaatagaaga tgggtttaac aaaaggacca gtcataggaa
    11881 gaaattttcg tgaaatcgaa tgataatctg aacggtaggc aaagactgtt aaaaagatgg
    11941 gaaaggtatg tgcgatcatt ttactgcacg attatttgga aaataccttg ttgttcgctt
    12001 tttctggaaa aaatacgtaa aaaaagataa aacgattgcg aactgcgtgt attcggtctt
    12061 tcgatctttt tcacggtatg gtatgatagt taagaatttt gagaaatttt aaaaaatagt
    12121 aatcgttaca caaaaatgtt ttttaaagtt ttcttatgat taaaaagtga atttgctgat
    12181 tgaacagtta tatgagacaa tattttagaa aatgtgaaac agaggtgtat tatgaagcga
    12241 tggcaaaaag ttctattaac cttgttaggg gtatttgttt tgctgattgc cggattatca
    12301 gcatacggaa tcagatttat gggagaagcg agccaaacag taaacaaaat ttctaaaaac
    12361 tctaatcgaa tatcctccaa acgttctgag agagtaagca ttgatgacaa agaacctttt
    12421 tcggttttgc tattaggttt agataccggt ggattaggaa gaacggaaca aggacgttct
    12481 gatacgatga tggtagtaac agttaatccg aaacaaaaaa aatcaacgat catcagtttg
    12541 gaccgagata tttatacgaa tatcgtaggt tatggaacgg tagataaatt aaatcatgcc
    12601 tatgcatttg gtggtgtcga aatggccatg gattcaattg agcaactgct cgatattcct
    12661 attgatcatt atgtaacgat caatttggat ggcatggaag atttgatcaa tgcggtcggt
    12721 ggcatcaaag tgaataataa aattgacttt actttggatg gtgtccatgt accagtaggt
    12781 gaacaaacat tgaatggaga aaaaggttta gcctattctc gaatgcgaca cgaagatcca
    12841 gaaggcgata tcggtcgaca acgtcgtcag cgggaagtag tgacgaaaat cgtcaataaa
    12901 gttttaagtt tagatggcgt aagcaattat agaaaaattt taaaagcagt tgagaacaat
    12961 gtgacgacgg acttagattg ggatgacatg cttgatgtag cgacgaacta cacccctgcg
    13021 tttgaaacaa tcaaacaaga ccagttgcaa ggcgaaggac aaatgatcaa tgatatctac
    13081 tatcaaattt taggaaaaaa tgatcttctt tcgatccaaa atgaactgaa gaaacaatta
    13141 aatataaaaa caagcgatac attacctaat ttgaaaaatg agaatgcttc aatgatgttc
    13201 tttgatgatt cggaagatgg cgattcgaca aacggtgatg gatcgaatag tgaagggtac
    13261 accgattatt cttcctattc agaaaacaca tatgatcagt accaagatcc gaatacctat
    13321 tatcagcaaa cagaagatac aagtcagtac tatacaccaa gtcaagatac tcaaagttat
    13381 gatccttacg ctggacagga acaaaatggt tatggttatg accagaataa tgagtatgga
    13441 acaacaagtg ggtactagaa aaaaatagtt tggttaataa agagtgccac ctaaattaga
    13501 caaaaaatct aacttaggtg gcactttttc tatgtgttaa attacaggac gtctcttttt
    13561 agcgcttagt ttaaaaaata aattgaataa ttcaatagag ttgcgaatag tacccataaa
    13621 taataaggaa tcatgagcca cattgccagg cgatgatgta gccacaactg atacataagc
    13681 actgacaaga aaataactaa aagtgtaata tcgattgctg caatgaaaat atttcttaaa
    13741 ttaaaaaata gagcagacca tataaaatta aaaatgaact gaatcacaaa aagaccgaca
    13801 agattgtttc gctgtttttt gttgttgtga ttaagtaaca aataaagaga aatacccatc
    13861 aatatataca gtaaggtcca catcggccca aatatccagc taggtggtgc aaatggtgga
    13921 agttgcaaag aatagtaata ttctccggga tttccgctaa gcaaccctga taaaaagcct
    13981 aatatgacaa ttcctataac acaaacccaa aaacgaatat cttttatcat cttcatttta
    14041 ctaactcctt tctataatag tagtttacca tgaaggctca cagcaagacc tgtatttagt
    14101 ttgtgaagta agaaaaaaat tgatgattgt tgttattaaa aagttgaaca tttgtttata
    14161 tgaatgatgt ttttgaaaaa gatcatatga tttggttc
//
LOCUS       DAXWLF010000064        13890 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXWLF010000064 DAXWLF010000000
VERSION     DAXWLF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.064"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            135..983
                     /locus_tag="ACGE4U_001431"
     CDS             135..983
                     /locus_tag="ACGE4U_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFF8393604.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1154..1495)
                     /locus_tag="ACGE4U_001432"
     CDS             complement(1154..1495)
                     /locus_tag="ACGE4U_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFF8393605.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1581..2288
                     /locus_tag="ACGE4U_001433"
     CDS             1581..2288
                     /locus_tag="ACGE4U_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFF8393606.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2275..2598
                     /locus_tag="ACGE4U_001434"
     CDS             2275..2598
                     /locus_tag="ACGE4U_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFF8393607.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2687..3724)
                     /locus_tag="ACGE4U_001435"
     CDS             complement(2687..3724)
                     /locus_tag="ACGE4U_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8393608.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4171..5154)
                     /locus_tag="ACGE4U_001436"
     CDS             complement(4171..5154)
                     /locus_tag="ACGE4U_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFF8393609.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5570..6340)
                     /locus_tag="ACGE4U_001437"
     CDS             complement(5570..6340)
                     /locus_tag="ACGE4U_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8393610.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6333..7268)
                     /locus_tag="ACGE4U_001438"
     CDS             complement(6333..7268)
                     /locus_tag="ACGE4U_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393611.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7862..9511)
                     /locus_tag="ACGE4U_001439"
     CDS             complement(7862..9511)
                     /locus_tag="ACGE4U_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFF8393612.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9706..10602)
                     /locus_tag="ACGE4U_001440"
     CDS             complement(9706..10602)
                     /locus_tag="ACGE4U_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8393613.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10595..11275)
                     /locus_tag="ACGE4U_001441"
     CDS             complement(10595..11275)
                     /locus_tag="ACGE4U_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8393614.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11276..12058)
                     /locus_tag="ACGE4U_001442"
     CDS             complement(11276..12058)
                     /locus_tag="ACGE4U_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8393615.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12051..12968)
                     /locus_tag="ACGE4U_001443"
     CDS             complement(12051..12968)
                     /locus_tag="ACGE4U_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8393616.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(12971..13255)
                     /locus_tag="ACGE4U_001444"
     CDS             complement(12971..13255)
                     /locus_tag="ACGE4U_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393617.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT         4110 a         2972 c         2441 g         4367 t
ORIGIN      
        1 taagaaaaga aaaggagaat gaatttttga caattcgagc aaaagtgatt atcaaatatg
       61 taaaatcagc tacttttttc ttaaacgttt ctttatacta aatataaata agtgtttcat
      121 gaaaggagaa ctagatgaat aattggcttg agtattttcc tgaaaacgtt ttggaacgcg
      181 gttacagcta tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc aagtacttgt
      241 ccgcaacagt ttctggcacg gaagattata aggtagtgat cacttgggat gaaaagacca
      301 acatgacttg tgattgtcta tatgccatag aaggaaaaaa atgcaaacac atggctgctg
      361 tcttattcgc ttacgaagaa cgtccaataa aaaaatccaa ctactcactt tccgaactct
      421 cgtcccttgt ttcctccgct agttcctctc ttgtacggga gcttttaaca gagattctta
      481 tagaacatcc gcactttatc gaacgcttca aagttaagat gccatttcat gcaataaatt
      541 attcagataa attaacaact atcatccata aatacgatca tattatcaag aaaaacaaaa
      601 acaggaaaac agcgaagttc atcatggaga tgcgaaagtt catccaagaa gcagttgaat
      661 cgcttattca gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa gtgattgcta
      721 ctctcgaaac attctattgg gagcctgagg atgaacgaac ccttcttctg atagaggact
      781 gttattattt atggaaagaa ttattagcag aagcacctca tgcagaaaaa aggcaaatgt
      841 tctcttggtt cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa cgctactcta
      901 taaaaatcct gaaagaagac tttcgggaaa aagaattttc taaccaaaag aaaaaaatag
      961 ataaaaaact aaagaaacgg taaagaaaga cgatttcaat gaaaaatagc aagtaaacga
     1021 taacaaccat cctaaatcca caagaaatac agcagacctt gctttaaaaa cacgaatcat
     1081 gcaaaggatg aagtaaagaa gacttaacgc gtacccaata ttagtcattt gactattagg
     1141 tacgcgtttt gttttataag tcatgacaaa tttccttcgt tgctggatag attttccggc
     1201 attgttgata gattttatga tacttttcta cattttgtaa gagaggttcc gctgcatctg
     1261 tatattggat aaaattctga caacaacttt caggtgtgtc gaaccaccct aaccctagtg
     1321 cagcaatcat tgttgcgcct aatgcaggtc cttcctccat acttaaacaa tagaccgttg
     1381 catcgaaaat atcagcttgg atttgtagcc atgcaagatt tttcgcgcct cctcccaccg
     1441 aaacaatacg tgtaaatctg cgacccgcta gttctttacg tttcaatccc accattgaca
     1501 aatgctcatt aaaaatttag aatattcaca agtttaacga caattatgaa aatttatgtc
     1561 gtgctaaagg agttttgtta ttggatgaaa agttagatat aaaaactgcg atcaaagaca
     1621 cgttacctac cgttttcggt tatatcggta ttggacttgc atttggtatc gttgggaaag
     1681 ctgccggatt tcatccacta gtcgtcacgt tgatgtccct gctggtctat gctggttctg
     1741 cccaatttat cacagtcagc atgcttgcta gtcacagccc attgctttcc atcgttttct
     1801 cgacctttct agtcaattcc cgaatgattc tgatgagtat gacgattgct ccttatttca
     1861 agaaaaatcg gctgcttcaa aatctgctga ttggcacatt gctgacagat gaaagtttcg
     1921 cactgggaat gaataaattg aactatactg gacaaaagtt aaactttcgt tggatgaata
     1981 cagctaattg gatttcttat ttgacgtggg tgggttcttc tcttgttggc gcacttttag
     2041 ggaattttat tactgatccg aaaaagtttg gtttggattt cgccatcgtc gctatgttta
     2101 tcgggttact ttatcttcaa gtcatttctg atagaaacac ctctaaacga ttacagttga
     2161 tcctgattgg tctcactttg attctggtat atgtcggact gatttttatc ccaagcaact
     2221 tagtgattgt agttgtaaca ctgattgggt gcggattggg ggtgtggatc aaacatgcct
     2281 tcttttgagt ttacattgct gactatttta ggttgtactt tagcaacttg gctttctagg
     2341 gtcctaccat ttgtcttact gaaaaaattc gatttaccgc aaccgttgct tgagtattta
     2401 agttttgtcc cgatcgtcat tatgtctgct ttatggttca gcagtctatt tacccaaaat
     2461 atcggacatc tgccgcagat caatctcgaa aatgctttgg cttcagttcc tacactttta
     2521 gctgctattc tttcaaaaag cttattggtg atcgtcttgg caggaattct ttccctttcg
     2581 ttgattcgct tattataaat aaaaaaacaa gagcttatgg caaattactg tcatgcgctc
     2641 ttgtttttat caaagtcaaa taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg
     2701 gaccactaac aacgactttt cctaacatat gtcctgtttc tacgtctttg gttgcttgtt
     2761 tcaagttctt cgcattcatc ccatctgaat agaccttgct caatgtactg cggataatcc
     2821 cttcatttgc taaagcagca atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt
     2881 tatagtctgt tttagcgaac atatactccc agtcgaagct gatagattgg tttttccaaa
     2941 cacctatatc taacggctct ttgatcccta cgatcgttcc cacatgtcca aatggtcgga
     3001 caaggtgctt gatttgatca aagtagcggg taatatcgaa taatacagcg acatactcta
     3061 cttgatcgat tcctaattca cgaaaacttt tacttaagtc gtgatgataa tcgattggat
     3121 agtctacacc tgtttttttc agccattcaa aattcttcgg acttgcagtc gcgtagactt
     3181 ctaggccagc ccaatgcgct agttgattca aaatcgaacc gacgcccccg gatccgttga
     3241 tcactaagat ttttttcccg cgattggcat tttcttctgg agttagacca aatttttcaa
     3301 ataacaattc ataagcggtc aatgaggtca acggcaaggc tgcagcttct tcatctgaaa
     3361 gattttttgg tgcaagagcg acaatacgtt cgtcaactag ttggtattct tgattgcttc
     3421 ctgctcttgt tgttgtacct gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt
     3481 cgtcacctac agcaacaact ttcccgactc catcaaaacc taagatacgt aaagcatttc
     3541 ttatcccgtt atcttggcga agtttcgtat cgacaggatt aacagatact gcgttgactt
     3601 ttaccagaag atcccgtgaa ctgggtacag gtatctgttc tttttcatca ataaagcttt
     3661 ttggatcatc gatcggcaac ccttcataaa aaccgactac cttcattaat tccttttctg
     3721 acatacgaat tcctccttag cttcatcaag ttggagcatc catacagtgt tcatttcaat
     3781 atatatagta tgctatttgt tccacctttt ttattcatca taattattct cttacttaaa
     3841 gtaaactatt tgcacttttt tggttaaaaa agaggttgcg acaagacttt tgtcacaacc
     3901 tcttataaac ggtgttctcc taccaactct tcggtcacaa gtcaaaatat tcatcaaaca
     3961 tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttaaacggca ggctcacaac
     4021 ctctgatata aacggtgttc aatcaactga ccctcggtat tagctcaaaa aacgacaaaa
     4081 tatgaagagc tatttttgtt tggctgttct tattacttga tacaggacag ctttttcacg
     4141 acctctaatt cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc
     4201 tttttcaaag gtctctaaga tctctcgtac ttcatcagta gatttcgtat tcatcaactg
     4261 aactcgcaag tcatttgctc ctggaaatcc tttgatatag attttaaaga aacgatgtag
     4321 tcctgtgatg gaacgcggga tgatttctga ataatgatct tgcagatcta gttgcagacg
     4381 aagtagtccg atcagttctt tttctgtatg ttccctcggg tctttttcaa atgcataagg
     4441 attcttgaaa atccctcggc caatcatgat gccatcaacg ccatatttct cggccaattc
     4501 ttctcctgtc tggcgatcgg ggatatctcc attgatcgtg attagtgttt gtggagcgat
     4561 ttggtcacgt aaagcaatga ttttaggaat gacttcccaa tgtgcatcta ctttactcat
     4621 ctctttacgc gtacgcaaat ggacagaaag attggctata tcttgatgaa gtaggtgact
     4681 gatccattct tccatctcag acatttccgt atagccgata cgcgtcttga cgctgacagg
     4741 caaaccgcca gctttagctg cttcaatcaa ttgtgccgct acttctggac gaaggatcaa
     4801 gccactgcct tttccacgtt ctgctacatt agggactggg cagcccatgt tgatatcgat
     4861 tccttggaag cccatttctg ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc
     4921 tccccaaata tgggcaacca tcggctgttc atcttctgta aatgttaatc ttcctcgtac
     4981 actgtctttt ccatctggat gacagaaact gtctgagttt gtaaactcag taaagaagac
     5041 atccggtgct gctgcatgct ttacgacatg gcgaaataca acatctgtta catcttccat
     5101 tggtgctaaa acaaaaaaag gctttggcag ctctttccaa aaattagtag tcatttattt
     5161 gttactctcc attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat
     5221 actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa
     5281 aaaaagactg tgacaaaaat cttatcacag tccattttcc ggaataaatg gtgttcaaaa
     5341 gcttgcattt gcggtaataa gtccaaccaa acgaaaaata ggaggagcta tttttgtttg
     5401 gctgttctta ttacttgatg cagaacagct ttttcacaac ctctccacta aacggtgttc
     5461 aatcagctga ccctcggtat tcgctcaaaa aacaacaaaa tatgaggaac tattttcccg
     5521 tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttttc taacttctca
     5581 atcctcttcc tttattgatc aaataccaag taacggcgta caaaacaacg atgaataaca
     5641 caagtacgat catcgatgtt gctactggca catcgatcgt tccaaggaat ccgtaacgga
     5701 aaccggagat catataaacg atcggattga cttttgaaac agcttgccaa aagggcggta
     5761 acatagagat ggcatagaaa acgccaccta gataagttaa cggctgcaag acaaatgtag
     5821 gcacgatcga tacatcatca taagactgag caaaaatccc gttgagcagt cctgctaatg
     5881 aaaacaagat tgccgtcatc aataatgtaa tgatgaccat gaaccatgaa tacacatgaa
     5941 gtggtacaaa gaacaaggaa atgatcgtta cgagagagcc gacgagaata ctgcgaccca
     6001 caccaccaat cacaaagccc caaataatca catgcgtagg aacaggtgct accaagattt
     6061 cttcgatatt tttctgaaat ttttgagaga agaaggacga agaaacattg gcatacgaac
     6121 tagtgatagc agacatcata atcaaaccag gaacgatgaa ttccatatat gaaaaaccac
     6181 ccatgtcacc gatccgccca ccgatcattt ttccgaaaat aacaaaatac aaggatgtcg
     6241 tgatcactgg cggaaccagt gtttggaccc atatacgcaa atagcggttc gtttccttga
     6301 tagctaaact ttttaatgca gtaaaataaa gattaaacat ttttctcctc cgtatgatta
     6361 ttttcttctg tgattttcaa gaacagttct tctaaacgat tcgacttatt cctcatagaa
     6421 agaactttga ttccttgtct tgtaagctga tcgaagattc cgttcacccc ttgatttcgt
     6481 tcaacttcga cagcgattgt ctgctcatct tcaaatgtac aagcatatcc ttcaatcaca
     6541 ggtttggtat catatggcgc taaatcaaag ataaatgttt caaattgcag ttttgacaga
     6601 agctctttca tacttgtatt ctcgatcagt tcacccgatt ggatgattcc gatgttgcga
     6661 caaagcattt cagcttcttc tagataatgc gtcgtcaaaa tgatcgttgt accattttcg
     6721 ttcaattctc gtagaaactc ccacatctcg cgtctcaact cgatgtcaac tcctgctgtt
     6781 ggttcatcca agatcagcaa acgtggttca tgcatcaatg ccctagcaat catcagtctt
     6841 cgcttcattc ccccggaaag catacgtgca cggacatttc tcttttccca aagattggac
     6901 tgcttcaaat atttttcgct gcgtttcatc gcttctttgc gagagacacc gtagtagcct
     6961 gcttgattca cgacgatttg ctggaccgtt tcaaaagggt taaaattgaa ctcttgtgga
     7021 accaatccaa tctgttgctt tgctcgaact agatccttgt ccagatcata accgaagaca
     7081 ttcacttttc cagatgtttt attgactaaa gaagtaataa tcccgatcgt tgtagacttc
     7141 ccagctccat ttggccctaa tagcgcatag aaatctcctt ctttcaccga aagatcgatt
     7201 ccccgcaatg cttcgacccc acttccatag atctttttca aattatttat ttcaagcgca
     7261 tttgccattt atttcctatc tccttataga catgattctt aattgtatca tggaatacag
     7321 cagatgaaaa aacaacttgg cttccacaca aaaacacacc tgctaacaaa gaggagatgt
     7381 gttttctagt tcatgatcat cttcgataga ataactatct aaacaactct ggaactttta
     7441 aaatcataaa agtaccgaca gcaactacta cgatgacata tagaagatcc ttataactga
     7501 ggttcttgtc ttttttattc atgttaagct ttcctccgtc cttttatcga aagctttttt
     7561 cttttatttt aaccgaaata aataaaatgg aaatagttat tttcaatttc tgttttcgct
     7621 gtttgtttta cggagaactt tgccatggaa cgtgcgtctc gtcgctgatg ctaagttttt
     7681 tcgataaaag ctgctagaag gaagacagcg tggtttattt tgtatcgtct tttgctaaat
     7741 cttccaagat catctggaca gcagactcgt tcccattgaa ttctgccact tttttggcat
     7801 caatgtactt cttttgataa tcgtcttttt ggacccacgc atcatgcttc gtttgataaa
     7861 gttaatcaaa cggataccac tgacgctgtt catcgaacaa caaactttct ttttgggcct
     7921 gttcaactat ggatttagga taattcaaaa ctttcagcaa ggcaatcgca tttcgggtcc
     7981 ttgaaggtcc ttcttttaat ttgaaatcaa aactgattcc ttttccttct gttacttgtt
     8041 cttcaaagtg gacattctca caataattct ttaaaatctc tgtcaattcg atatcatgtg
     8101 tggcgacaaa tgctaaagat ggatactccg caagccaccg aacgactgaa gcggaagaag
     8161 caatccgttc gatcgtattt gttcctttta aaatctcatc tacgaagcag tagcagcgtt
     8221 ctttggaagc cacttggtcc agcaaacgtt tgatcgattt gatttctgaa acaaaataac
     8281 tgtcgccttc aaataaatca tcttcgattg ccatggaagt caacacatgt ccaggttgca
     8341 gggtaaactg ttctgcaatc gctgtttgga tcgtctgtgc tagaatacag ctgatggcta
     8401 tacttttgac atacgtagat tttcctgatg cattggaacc cgtgactaat gtattcttct
     8461 gccagttgac tggattaact accgcttctt ttaacaatgg atgatagata tctttcgctg
     8521 tcacaccgcc ctcagtaaac tctggtcggc aagtgatcgg catataggtg cgataattca
     8581 aaacggctgc tgcaacttcc aattctccca gcaattccca cagcttgatg gcttcagttg
     8641 aatgcttttc taatttagtg agaacgaaat gataggagat aaatggcagc atgaacatga
     8701 tgttgacata ttcaaaaatc aattctgctt cactcccgct cttcattcga aatgaaaaac
     8761 ctaatttagg gatgtctctt aatggtttag tttgctgttt cagctctttt tgaagtggtg
     8821 tcgtcatttt tgctaactga ttggcggctg aaatcgttga aaccaaataa ctcatgctat
     8881 tcagctctgt ttctagtgtt tgtttcttca ctagataata gaccgtattg aatactaagc
     8941 caccgattgc tagatagatt cctagtactt gccctaaaag aagcaaacct acacccacca
     9001 gcggaagcaa gccaagaaaa ataaataaac ctacgcgtcc aatcttttta ctttttccat
     9061 ccgctagata ttgttttgca aaattatgat ctttttttcc taaacgagcg aattgatatt
     9121 ggattctttc tctcagttga ggattttcct gataaaaagc aattagtttt tctaactgat
     9181 aatcttctcc aaaatcaaag ctacgtaaac gctgatacaa cgcttctgaa cctacactag
     9241 aataggtact gttgatccct tcaaatactt cgaacatatc caagtcatac caagtaagat
     9301 cgtcgatttc actatcccat gaacggaatt ttttctccgt gagccacgca tccttcaaac
     9361 tttcttcttt atcaaatctc ggttgataag gaatttttcc ccaattacta cgaactgttt
     9421 cctttacttt caaacgtgaa aaaatgtcta aaacaactaa taaaataaca actgcaataa
     9481 taattaaaac gataaattct ctttggccca tatccctctc cgttccttga tttgttagtt
     9541 ttagtatagc actgttgcgc tatttgaaaa gggggtacaa aaataactgt cttcgttacc
     9601 taattcgtat caatcacacc aaaataaaaa caccttccaa aaaagaagat gtctttaaaa
     9661 cgagatacaa ttttttgtca ttcacttcag gtaaattaga gccaattaaa ccctccgcaa
     9721 cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc ctcccattcc
     9781 ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat ttgtgcggct
     9841 ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg ctcctggttt
     9901 gataggattg gccactgtta atcgaatgaa tgaatcctct tttgaaaatg tcacctttgc
     9961 ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc gcagcaacat
    10021 gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta cctcgatatt
    10081 tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt tagaaaaaga
    10141 aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat cataaaactg
    10201 atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat acttctcccg
    10261 tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag cagtcaaagg
    10321 agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt ccatttcttg
    10381 aaatgcttgt cctttttgtt cattttcatc cacccatgta ttcagtgcat ctactaggtc
    10441 cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt gtcgttcaga
    10501 aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga aaataaaact
    10561 tcccataaag ataacaacga gaagcaacag ccacttaatc aaatctaaac cccactcccc
    10621 acaccgtatg aatatacaca gcatgtgcct ttgctagttt ccctcgaaga ttggaaatat
    10681 gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt tcataaagat
    10741 ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga tcgaattctc
    10801 ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct ttgtccagta
    10861 agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa gtctgatgac
    10921 gaagttggat gccgattcgt aacaacagtt cttcgatatc aaatggtttc gtcacatagt
    10981 catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt gctgaaagaa
    11041 cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca cctgaaagcc
    11101 ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac aataattttc
    11161 cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc tcctccagta
    11221 aagaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg aacacctact
    11281 taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg attgttacta
    11341 gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca aagtccgtat
    11401 acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg atttttgcga
    11461 aaacaccggg aacgaaggcc gttcccatga tataaagaaa agaaagaagc aaactgacac
    11521 tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtcgct aatatcagca
    11581 gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt ccctcaactt
    11641 caccaaatcc gaaagcaagt gtgtaagcaa tcatggctag ccccgtaatc aaaaccacgc
    11701 caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc attcgattcc
    11761 gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca atataaaagc
    11821 acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttccacc attaaaatcc
    11881 ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg tattttgtat
    11941 gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt cctagtgaga
    12001 cccacatttt acggctcttc catactttta ttttttcagc agttaataga ttaaacatgt
    12061 ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac taggtggata
    12121 ccatccacag gaacttttgc ctctaccaat gcttgattga tggctgctac ttggtcaatt
    12181 ccttcaaaaa gctggataac acggctagat tggacaaaat aatccctata accaagagag
    12241 tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa ttgacgagat
    12301 tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg caaaataccg
    12361 tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa gacggtgatg
    12421 ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc tgctggatcc
    12481 atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag tgcatttgca
    12541 atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt ccctgtattt
    12601 tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc gccgttcgct
    12661 gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg ggtttcaatc
    12721 gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa ttcgatcgaa
    12781 ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt acccgctcca
    12841 ttctccccaa tcaaaccata gatcattcca cgtttgattt cgatcgatac tttatctaat
    12901 gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt ttcatttcca
    12961 aaatccatac ttatccttct ttcttattga taagtgaagt atagttgacg ggtataaaga
    13021 aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatttca agaaaaatca
    13081 gactgtctcg cagacgtggt tgagttgtct gccaatcaaa gagcgcacca aacagtgcta
    13141 atccaagtgg actggccacc atggtaatcg ttgttatcgt tgacatcaga cgtccttgta
    13201 tatgcgttgg aacttctcgc tgaagccaga ccatataaaa agcaaaagaa aacattaatc
    13261 cttaaaaacg gacttatgaa aaataataca tgccgtttgg gatttacaat tagctaccaa
    13321 ataagaggtt atgacaaaag acttttcaca atcccttctt ccgaataaac ggtgttctcc
    13381 tgccaactcc tcggtcacaa gtcacaatat ttatcaaaca tgagaagcaa tttttgtaaa
    13441 ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcagaagcg gataaataat
    13501 attgtttgat gatactttat ctttttcccg tttgtttata gatcgaatga tattatagac
    13561 caagaatcct acctattaac atagctaaaa acacaaaaaa ggcactttcc aaattgtgga
    13621 aagtgccaga aaagttagga ttaaggtaac tgcgctcagc attgcttcgc ccagtacgat
    13681 tctcgtaact cgtgaactcg aacgataatc gcaattaacg ttttgagaac tgtgaagctt
    13741 tacgggcttt cttaagacct ggttttttct ttatatcatt gaatagcctt gaaaaacctt
    13801 gatataaaaa ggttttagta aaaatgattt tatcaaattt gaataacttt gaaatatttg
    13861 tgttctaaaa tgtgttctaa tttaaaggta
//
LOCUS       DAXWLF010000065        13878 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXWLF010000065 DAXWLF010000000
VERSION     DAXWLF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.065"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..184)
                     /locus_tag="ACGE4U_001445"
     CDS             complement(<1..184)
                     /locus_tag="ACGE4U_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFF8393618.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLR"
     gene            complement(663..1382)
                     /locus_tag="ACGE4U_001446"
     CDS             complement(663..1382)
                     /locus_tag="ACGE4U_001446"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8393619.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1431..3077)
                     /locus_tag="ACGE4U_001447"
     CDS             complement(1431..3077)
                     /locus_tag="ACGE4U_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFF8393620.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3256..3846
                     /locus_tag="ACGE4U_001448"
     CDS             3256..3846
                     /locus_tag="ACGE4U_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8393621.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3938..5443
                     /locus_tag="ACGE4U_001449"
     CDS             3938..5443
                     /locus_tag="ACGE4U_001449"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFF8393622.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5527..6879)
                     /gene="celB"
                     /locus_tag="ACGE4U_001450"
     CDS             complement(5527..6879)
                     /gene="celB"
                     /locus_tag="ACGE4U_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFF8393623.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6879..7397)
                     /locus_tag="ACGE4U_001451"
     CDS             complement(6879..7397)
                     /locus_tag="ACGE4U_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393624.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7413..7739)
                     /locus_tag="ACGE4U_001452"
     CDS             complement(7413..7739)
                     /locus_tag="ACGE4U_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFF8393625.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7752..9731)
                     /locus_tag="ACGE4U_001453"
     CDS             complement(7752..9731)
                     /locus_tag="ACGE4U_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8393626.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9752..10066)
                     /locus_tag="ACGE4U_001454"
     CDS             complement(9752..10066)
                     /locus_tag="ACGE4U_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFF8393627.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10234..10527
                     /locus_tag="ACGE4U_001455"
     CDS             10234..10527
                     /locus_tag="ACGE4U_001455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFF8393628.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10605..11717)
                     /locus_tag="ACGE4U_001456"
     CDS             complement(10605..11717)
                     /locus_tag="ACGE4U_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8393629.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11870..12718
                     /locus_tag="ACGE4U_001457"
                     /pseudo
     CDS             11870..12718
                     /locus_tag="ACGE4U_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13071..>13878)
                     /locus_tag="ACGE4U_001458"
     CDS             complement(13071..>13878)
                     /locus_tag="ACGE4U_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8393630.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4334 a         2836 c         2373 g         4335 t
ORIGIN      
        1 ccctcaagct agccaagaac ccatctgctg aaatatgaaa atttttgaat gctacgacat
       61 tcgccattac aggtatgtaa aagaaaaaaa tcatccaaag catacccggt acagccatca
      121 ggacaagtgc tttataacgc catagattca taaaaaaatg attgaacccc tttcgttttt
      181 tcatctaaaa acccctttcg attcattcgc aacgtaatta tagcgctttc aataatgaga
      241 ataaataaac aaagtatagg gaaaatgaaa caaagtatag gagaggttgt gaaatcagcg
      301 ttttggcctg agtattagag aaaaaaacgg gaaaatagtt cctcatattt tgccgttttt
      361 tgagctaata ccgagggtca gctgattgaa caccgtttat cagaggttgt gaattcagcg
      421 ttttggcctg agtattaggg agatcgtgtc cttactatct aactctgagt cacaaggaac
      481 aatggggact gtaacaaaag tcttgtcaca gtcccttatt ccgaacaaac ggtgttccag
      541 aagtagcttc tacggaaata agccgaaatt tcacaaaaaa gccatccgaa agtcagacgg
      601 acaagtctgc ttttcagatg gcgctacaac tactctgctt attcttttag ctatccattc
      661 aattattttt ctctcacttg ttctagctct tctttggtca tgggtcgata ttgcccttta
      721 ttcaaatctt taggcagcca aaattttccc atacggattc tttttaaata taagacttct
      781 tttcctactg ctttgaccat tcgcttaact tggtgaaact ttccttcatg caaaatcaga
      841 cggataattg aagtttctgt ctcttgatct acttgatcaa tttggagttt tgccggcaat
      901 gtctgctcat cgccatcaat aatcagtcct tctgcaaata aacggatatc ttcttctgtc
      961 attataccgc taacctcagc tagatattct ttctccacat gttttttagg tgatagtaac
     1021 tgatgggcaa acaccccgtc attcgttaaa atcaagaggc cctctgtgtc tttatctaat
     1081 cggccgactg gaaaaagatc ttcccgataa tcctggtctt ccaataaatc gattacggtt
     1141 tgatcatgat tatcctgagt agcagaaatg actcctgcag gtttatgcaa aatgtagtaa
     1201 aaatcttttt gatacgtaat gatttctcct aaataagtaa ccgcatcttc gaattcattt
     1261 acttgcgctt tatcgctttt caccacttgt ccattgactt cgatcgttcc ttttttgatt
     1321 aatcctttga cttctttgcg gctgccaagt cccatatctg ctaaaaactt atctaaacgc
     1381 atgcgtttcc cccatttcaa aagagcatca atctagctct tctgattcac ttattttatt
     1441 tttaatttat gtcgtaaacg cgctattcct ggtcctagta gtctttctgc caagcggatt
     1501 ttcaaagcta aataaccata cactaaagcc cctatacctg ctactacaag aatcaacaat
     1561 agagactgga atttgctatc ttcgtttaaa aaagcaccaa aaatcatctt tgtgatccac
     1621 gaagccaaaa gcataatcaa ggtaagaata aagatcagca atgtcctgcg ccagacaaaa
     1681 cttggtttaa agcgggctac ttcatggatt ctctttaaga tcaaggtaca agacacagca
     1741 aatccgatca ttgtcgccaa taaagggccg tatacttcaa ataaacgaat ggctgggtat
     1801 tgaagaagga gtttcactag aaacccaata accaaataat taattgccgc cttgttttca
     1861 aacattcctt gcagcatgtt ggaaactagc atatatagtc ctaggaagag tcctacaaaa
     1921 cttgcttgga tcagtacatt acttcctaga gaatccggtg tataaaataa tgtatttagc
     1981 ggataggcaa gtaacataac accaaatgtc gccggaaaca taaaaaatga aaacaactgt
     2041 aaatttccgc ttgtcaattt tgccagacct actcgatctt tcaacgtaac agcttctgta
     2101 atcaacggta acccagtaga cgcgatcgat gttgccaatg caataactac catcgtcaac
     2161 ttatctggat tggcactaaa aatcgaaaaa agatccagta actgtgcatt ggaatactcg
     2221 gtggatgcag acatgatctt cataaaagtg aactgatcga ctaatttgaa gatcgttacc
     2281 cctgaaccca caataataaa cggaatcgct tctttaacgg tatctaacaa caattccttc
     2341 gttgcaatat ttattttgtt ttcgctgtag tgaataaacg ccgatgtata cgcttgatgt
     2401 ttcttcaaaa aatacaaaag gacaagaata cttgctagca taccgataaa tgccgcgaaa
     2461 gttgattgcg tgaccgccgt cacatagtca ccatccatga ctttcatgat gatgaatgta
     2521 gccaatagca tataaaaaat acgtgccacc tgttctacaa tctgcgacag cgcataaggc
     2581 atcatttctt gattgccttg caaataccct cgtatcacac tcatacaagg aaaaaccaat
     2641 acagcgatac tcaaagaacg catcgttgga atcagttctt ctccgccacc agaagcatga
     2701 gctagccaag gagaggcgat gtacatgaac aaagcaaaca acaggcctaa cccgcccatc
     2761 atctgtaatg cacggataaa taatcgtcgt gaagtgccat attcattcaa tgaattatag
     2821 cgtgcagttt gttttgcaat cgcagcaggt attcctgctg tagagatcag tagaaacaat
     2881 gcatatatat tgtatcccat attgaataat ccattggcag ccttagcatt ttcgcccatc
     2941 caagcatacc agggaatgat atagacagcg cctagcaatc ttgaaatgat attactggcg
     3001 gtcatccaag cagagcctcg tgccattttt tcttgcggtg ttaattgatc taatgctggt
     3061 tttctttgat tatccatcgg tcagattcca actttctaca aaacattact atcattttaa
     3121 ttggtaaaac agaaactggc aatcgatttt atataacttt tataagaaca attgtttttt
     3181 tgaattcggt ttcgtttttt tggtaaagta aagaaatata cagcctttta tggctaacat
     3241 ttataggaga tatcaatgac acaagaaata aaaaaactac ccgttatatt tttaggtctt
     3301 ttctttatca cagttggatt gaattggttc ttgctccctc acgatatagc ttccgccgga
     3361 gtgggcgcca tcggacattt agttgaaacg gccttttcta tccaacgctc agttactgtt
     3421 tggagcatca atcttatgat gctgcttcta gccgctgtcc tattaggaaa atcagtattt
     3481 tttaagactg tagcgggaag tctgcttttt cctgttgttt tagagattgt tccaaaagtg
     3541 gaaatgatct cctcacattt cttttcactg atttcaggaa gtcttttgtt cagtcttggg
     3601 gtttacacat tgtacacagt cggggcctca aatggcggta taacgatacc gccgattatt
     3661 ttccacaaat tttttcggct tccaatgccg caaggacttt tacttaccaa tagtcttatc
     3721 gtctttttga attattatgt ttttggattg ttggagacac tattcgttct cctatctatt
     3781 acagtcagtt cattgtttat gaaaatactc atccgcttgt cccctgtgtc aagaaaattt
     3841 gaataaagct ggattttgtc atagcggaaa caaacgatat attaaaaaga gtgtgctata
     3901 ataaaccaag aaattgaata aagcgaggga tcaatcaatg agcttctctt tagaagacat
     3961 ccgtcattta ctattaaaag aaaatctatt aaaagaattt gtttcaacaa gaggctggca
     4021 tcttgatgta ccagaaaaag ctgaatttct tcagttaagt tatgattcaa gaaaagctga
     4081 tgcttctact ttgtttttct gtaaaggaat gaatttcaaa gaagaatatt tggattcagc
     4141 aatcgaacag ggtattcaat attatgtatc agaacaacca tacgaaaatt cagcagtagg
     4201 aatcatcgta acggatatcc gtaaagcaat ggcgcttctt gctatggctt tttacgacta
     4261 tccgcaaaat aaattgaaag tgatcggatt cactggaaca aaaggtaaaa caactgctgc
     4321 gtactttaca aaagcaatct tggatcacac aacaaataaa aaaacagcat tgctttctac
     4381 tatgaataca acgttagatg gcaaaacata tttcaaatcc catttgacaa cgcctgagtc
     4441 attagattta tatcgtatga tgtcagaagc agttgaaaat ggtatgactc atctagtgat
     4501 ggaagtttct tcacaagctt acaaaacaca acgcgtatac ggcttgactt tagatgtcgg
     4561 aatcttcttg aatatctcgc ctgaccatat cagtccgatc gaacatccga catttgatga
     4621 ctatttctat tgcaagcgac aattgatctt gaattcaaaa acagttgttt taaatcatga
     4681 aagtgactat tttgatttac ttagggaaac agccgatcta ttccagattc caacgatcac
     4741 ttatggtcgt tcggaagaaa gcaattatca agtgatccgt tcagataaag gtactcacgg
     4801 ttttacttta tcttctcatg aagatcgctt agctatctgc gatactgctt atgatatttt
     4861 gctggctgga ggattcaacc aagaaaatgc agccagtgcg atcattgccg cagcactaac
     4921 aggtgcctcc agtcaagatg cacaagacgg attaaaagaa gctcgtgtac caggccgaat
     4981 ggatcaattg atccaatcca atggagcgca tgtctatgtg gactatgcac ataattatct
     5041 aagcttgaaa acattgctga aatttgccaa aaatgaacat ccagatggcc gtgtaattgt
     5101 tgtattaggc agcccaggaa acaaagcaat ctcccgtcga catgattttg gaaaagtgct
     5161 atcagaaaca gctgacatcg cctttttgac ggcagatgac ccagcatttg aagatccgaa
     5221 aaaaatcgcg gaagaaatca atgaagcaat tactaatcca gatttgatcg ttcattatga
     5281 aatggatcgt cctgaagcga ttcgacgcgc tttagcagaa agcgctccgc aagattcagt
     5341 cgttattgcg ggaaaaggcg tggatcccta tcaaaaaatc aatggtgtag atgaacctta
     5401 tgaaggggat tatgcaatcg tgaagcggtt gattgaagaa taatggtatt tatatactag
     5461 tggtttccca tttctaagaa aaaaagggac tgtgattttt tgtcacagtt ccttttttct
     5521 tagaaatcat tttaactttt cactagctaa ttctgctgcc ttttctcttt cttcatttaa
     5581 taatttatta tcatagaatt tgataaatgg gaaccagata atgaatgcga tgattgcaca
     5641 aatgactgct aacacagcag ctttccaatc accaccacta ccaacaaatg ctccgatccc
     5701 gactggtgaa ggccacggca tctgtgcaag catcggtttg acgatttcca gccgaatggc
     5761 aaaataagcg agtgtagcag aagccatcgg agctaagaaa aacggaaccg ctaaataggg
     5821 attgtagacg attggcatcc cgaaaatgat tggttcattg atattgaata atcctggtac
     5881 aagtgaggct tttcctaaca ctttcagctg atcagatttc gcgaggaatg caataaagat
     5941 gatcaatcct aaagtcgcac ctgatccacc aacggttaca taagaattgt tgaattctcc
     6001 tgccaacgga taatttgcgc cttgtgcgtt agctgccata ttagctaata cgatcggggt
     6061 caagaaagaa gtgatgatat tcgctccatg gatccctacg atccacaatg catggatcaa
     6121 gaaataaatc accatgacac caagccatgt attcgtcaac tgcgtaacaa acccaaatgg
     6181 gatagcaatc attttgaaga tatccgttcc tagtgcaact aataatccat tgatgatcat
     6241 caccacaaac gcaatgacaa atgttgggat cagagcagtg aaagaacgag aaactcctgg
     6301 cggaacaaca tctggcattt ttatgaccca atttctcttg acacacatac aatacaattc
     6361 gaccgcaata atcgacataa taatagctgt aaagattcct gaagttccta aacgttcgac
     6421 aaaagagccc atcctaatac catttactac agtttctgtc tcattgatag aagcaatcaa
     6481 gctgatttta ccatcagtca tgatcaattc agggatacac ataaagaatg caaaaacaga
     6541 tagtaatgcc cccgtgagcg gattaacatt taatttttgt tcatctcgtt caattgacgt
     6601 taattcataa gcaaacacaa tggcaaagta aatagataaa atccccatcg tcattgtatt
     6661 gacgatcatg tacaagtcac taactttgaa aaaagttgca ttaaaaaatc cttctaaaaa
     6721 tgggaacggt aatggcaaaa tatttaaaac caaaaacatc gagcctacaa tcgtgaaagg
     6781 aatagaagcc atccccgctg ccatgacagc ccgaacgatt ttaagttgcg caattttccc
     6841 cataggtccc atcaggtatt tctgcaaaaa atcaaacatt agttattctc ctttcctaaa
     6901 tataacgctt tttcatattg tttaatacgg atatactgtt ggtcttggtt gagtgaaatt
     6961 ttctttaaac gatactgtat aaatgcactg gcaaaaatac cgcttacaac aagaatgaaa
     7021 acaaataact gtcctccatt gttatttgcc ataaaaggat acaagaatgt aaatcctgag
     7081 gtaaacaaca ctggaatcac taataaatta aagacactct ggaacaaaaa ataccacttt
     7141 gtccatggaa cgttcgtttg gtgttttcga taaagcttta cttgttctcc tactgcaggc
     7201 aaaatagcta aaatcagaat catcggtaaa ctacttccta aacttttagc tgatagaagc
     7261 agaatcatcc attgcatgtt catgaaaaag aaaaaggcag tcaggtaacg tacgattaga
     7321 taacgattga aatacatgtt tttcaaacta agttgctttt tttctaattc gattttcctt
     7381 tttactgtat cctgcacaat gatccttcct ttctatttat ttttttgata caaataattc
     7441 atttcctgtg caacttccaa taatgtcatc gtcgtcatca gatgatcttg tgcatggacc
     7501 atgataattt ccatttctat ttttgtcccg ctggcatatt ctttcagaag ttgagtttgt
     7561 gactgatgcg catgaagaat ctcttcattt gcttgtcgca acttctcttc tacttcttca
     7621 aacatctctt tacgcatcga gcgaaatgct tcatgtatca gtgtccgtgc atttccgcta
     7681 tggaaaatga tatcaaaaga agtcacttgt agtgtttcac tgtttatttt ttcagccatc
     7741 tgttttcctc cttattcgat cagtttgata aatttactgc tgaaattctc aaaaataggt
     7801 tcagctaaga tttcctgctg tacttttagc tggtcaacta acctaacgat tgctttagtc
     7861 acgaccgtaa ttccttcatt ttcgacataa gaaggcgaaa ctaaaaaaac aaaatgaatt
     7921 tcttgatatt gatcccactg catcccagaa ggaatcaatg caacggctac ctttgtttcg
     7981 attccaactg gaacagcggg atgtgggact gccactgtat cactgaatat gatttgtccc
     8041 atattttcac gttgctggat ctgctttcgc atttctctta tatatccttc ttcttcattt
     8101 atgctcagct tttgcagcaa atcatgaaga atttcttcct ttttcggttc gtacgtataa
     8161 gtcttgaaaa atttttcaga caactgttcg gtgtaaatct gctttttttg ctgtagagaa
     8221 aatgctggta tcggtctatc tgaggaatga tagggtatag attctccaac tacctttcgt
     8281 attttacgaa catcatcttc attcaaaaaa atactgacat gtaaaactgg aattttgaaa
     8341 aacattgtcg aaagatcaat agaagaaata atcaggtcga ttccttttaa agttgtttca
     8401 ttgatctcat aatagccttt tacacttacc actgtaatat ttttaccgaa ttcactgact
     8461 acgcgattct ttaatagttg ggcactgccg taacccgttg cacagataat caatgcatgt
     8521 agtttatgcg cagctctttc tttttccaat gccgccatga gatgcaaagc caaataggcc
     8581 cattcatctt cattgatggt atagtttttc aaagccggca tgttgctgaa atgccttttt
     8641 actgcgtcaa agactttggg attttctttc tttatctcct cagtcaatgg gttctcaatg
     8701 cgaatacctc ggctcaatcg aatcatcatc ggtgacaaat gatcgagtag tccattgaaa
     8761 agctggagat cctctactaa tgaaaaatga aattcctgag acaatctttt cagaactttc
     8821 gctaattcct ttgatagttc ctgatcattt tgattttctt tgtgattcga cttagccatg
     8881 agatgtaatg tcaaataagc aatttcttct tcgggaaacg atacacctgc gattacctca
     8941 atacgttgaa cgatccgctg cgccacttga tactctactc gcttagaaac ttcttgttcg
     9001 atgcctaatt ccttgatttc aaacccttct cggagccgtt tgatccccaa agccaagtgt
     9061 aaagtcagat tctgtatgat aaaatccgaa acttttaact tagcttctct tgtttcatcg
     9121 agaatgataa tgactagctc ttcaaaactg atgtctggga aaaaacggct ttgccctaaa
     9181 tattctttca atgaatttcc ataatggttt ccaaaaaaag tatccatgat aaaatgccgt
     9241 cgatttcttt cttccccttc gatccatgtc ccttgaccat gcttagaaac gatttttagt
     9301 gaatatggaa tcaatcgctc cttgatttct tgcatgtcct ttgtcaaact ggaacgactg
     9361 atataaagct cttctgccaa atcatccata aaaagaatgg catcttccaa aagcaattgg
     9421 ttcaatatat acttttgtcg atcttcggac tcataaaatc cttgcggttc tctagcatat
     9481 ccaggtacta tttcttgccg actcaaaaac aagtcaaatg tcagtctttg taaaatatgc
     9541 agttggtagc cataacctgg tttagcaatg atcttacctc cgtttttttc tactaactct
     9601 ttgatccgct gcaagtaatt acggattgtc cgatcagata ataacaattc agaagctaaa
     9661 cgttgactgg tgacatactc cttcttgtga tcaatcaaat atctcaacag caatttttcc
     9721 ttctgtttca ttatcatcca ctcctattcc atcatatgtt ctcttcgatc acctctgcta
     9781 acttctggat gcctgtaggg attgggacat acgcttggaa agggatacta acaacaggct
     9841 ttccaacaga ttctcctaat tttttgaatt tatccaaaaa catcgtcgtt tgcgggctga
     9901 tcagaaataa atcaaaatca ctattcttaa tcatattatc gccttctgta gctgaaactg
     9961 catctacgag tacctcttct cttttttgct taaagaaatc cgttgtcttt gaggccatca
    10021 tcgaggatga cattcctgcc gcacaaataa tcaatgcttt tttcataaat caatcactcc
    10081 ttttattttt ataaaccaag catatcttgt aagcgttttc tattcaaaat atatatttcc
    10141 gtagcattgc ggaaaaaatg tgtatcatat cttgaataat aatttctggc accgtgatat
    10201 actagtgata gaattttttc aggaggggat ttagtgagta tcacagtgaa tattttgtat
    10261 acaagtacaa atggacaagc cagaaaattt gcagaagaaa tggtcgaaag aaacattgtc
    10321 aaagcgattc gtgcagaaga aggaaacgag aaatatgact attactttcc actagaagat
    10381 ccagaatctc ttttactgat cgaccgttgg aaagatgaag aagcaatcga aaagcatcac
    10441 aaatccgaaa tgatggctca aataaacgaa ctccgtaaaa agtataaatt aaaaatgaaa
    10501 gtcgaacgtt tcactgattt accgtaaaaa gatacacatg gaatgatagc aatttatcat
    10561 ccatgtgtat cttcactttt tccttatttt ttattcgatc tcatctaatg attcaaaccg
    10621 tgtttttctc ttatttttcg gataagtatt tcagtcaccc caatatacat gattcctaaa
    10681 cataccccaa ggatattctc aaaaatgcgg atcgttactg ctcctggaac cccgaataaa
    10741 ctggcagtga ttgccaaagc accaaaagaa ttaaaaactg ttttatatct ataggtagaa
    10801 caaacaccta aagctagacc acctaatata ccgacccagt taaaaggaat aaattggctg
    10861 ataccaataa ataaaacaca tccaatcaat gagccaatga ttcggtctac tgctcgttct
    10921 ttgaaaccaa tcgacatcaa tccatagcta gatacaatag aagaaaatgc aaaccctgcc
    10981 cacatcaatc gctgaaaagg gacatactct cctgcaacga tcaaaagact aatgccaaat
    11041 gcatagatga gcatccaaat tttttcctgc gaatacatgc ctttcaaaaa aatctcttta
    11101 aacaagcttt tgtctttgtt cttttctcga tgttttctgt aaaggagaac tgaaaaccaa
    11161 caaaaaaaca aaaataataa gctacttgtt tgtgtaaaga aattcttgtt caacgaatct
    11221 ttaggtaacg aataaacaat aaagagatat gaaaagccgt acaaagaagc attccccatt
    11281 ttgggatcac tagcggttgc cattaatatg ctgcttagta aaataaaatg aatcaacatg
    11341 cccataactg accatttgat cagctggaca tacggtgcta ctaatagtga gaacatgacg
    11401 attgctaaac ctatgatcga atgggaaatc ttataaccaa agtcaacaaa acgaatactc
    11461 atcaacatac agaataaaac cacagcatat ggtttatgct tcgattcaaa taatgttgta
    11521 attataatga taaaacaaat ggcaaacaat acgatgaata atgatcgtaa aaacaatgaa
    11581 caaatataca tgcacttttc ttttttagaa gctgcttctc gtattttttg ttttaaaata
    11641 aacggatcta gttgtagaga ctgataaaaa tccacctgtt gtcgtccttt ctttttactt
    11701 ttatttttta cagtcatcta tgacaggttg taaatactat ggaacatcat atggcgttgt
    11761 cattttcttg tcaactattt ttcctattca tgtttttata aaaaaacata tcgttttctt
    11821 gtatgattaa atcaccacaa aataaacaac tgagagaacg atatgcttta tgaatggttc
    11881 tatcaaaaaa attctgagat caatagacaa aaatttgatg attacagagg tctcttacga
    11941 aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt ctcttccctg ctccatgtgt
    12001 ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg aaattaatgg tggtgaaaaa
    12061 caagaagaag gaaaccattg tccactttga ataatataat catatgccaa tggttatacg
    12121 cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc cattggaacg ctcaaagtta
    12181 ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga cataaaatca cttctttact
    12241 gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt taggtatctt tgacaaccgt
    12301 tattcgcaca ttgaaagagt tgagaagcta tttaccaaag tcttccaaaa gaattctgtc
    12361 ttgagtgttg agggttgtga atttcgttct cacgtttccg ttgaggataa actgagtttt
    12421 atttccgcgg atggcgagac agaaaaatta atcgagaaaa tttacctaca caaaaaattc
    12481 ctcgattaac aaactatttc cttagctgca ctaatcctaa agaaatagaa ttcttagtta
    12541 cagacatgaa cgccacctac tttcagctca ccaaacatgt tctaccaaat gcgaaattgg
    12601 tgattgatcg attacttggt ttttccacgc ctttcaagga ggcgtaacct ttattcacga
    12661 gttggttgaa gctttccgag acaaaagacc cgacttgttt ttctccttat tgacaaagag
    12721 ccattaaaac tagagaaaaa attacaactg atcgctgatt atttgtagaa agaaaatcaa
    12781 agtattaccc aataaacttg agtcattagg caaaaaacac tgcgaaactc aagaatgatt
    12841 ttcgcagtgt ttttgtatat tttttattaa ctagtattac tacaaaggtt tatttttcac
    12901 ttctcaacgc agcacaaaat tgtctttact tatttactgg ttgggttcaa ccttctctac
    12961 tccgatacta atatttgttt gtatacccct tctaaaatcg gtagtttcca ttcaattgat
    13021 gggtactagt tgattttaaa aactatgctt attcaatttt ttattttagc tcatatattt
    13081 cgtttctttc taaattttat actgatgaaa acaagtagca cacccaatat tgacaatata
    13141 aactggctaa tgatggtttc tccagttttt ggaagatgct tatttccact gcttgttgga
    13201 ttgtttcctc ttatatctga gctatcagca acttttctat tctcttttgt taaaaagatc
    13261 gccacttctt gttctttttc gatcttgaaa gttactggtg tcgcatccaa ttcatacccc
    13321 gttggcgctt gagtttctac cagctgatat gctcctggtg ctaagccatc tatcgccagt
    13381 tttccatctg ctcctgtggt tagacctgac tgcagaacgg ttccattggc atcttgtaac
    13441 tcaaaaacag ctccttgcag gacttctccg ctttggtcat cgatctttgt caacacgaca
    13501 ccgcctggtg taagtcgatt ttctttcgtc aattctacca ccgctgtctg acttctttcg
    13561 atctcaaact cgattggtgt cgcatccaat tcataccccg ttggcgcttg ggtttctacc
    13621 agctgataca ctcctggtgc taagccgtca atcgccagtt ttccatctgc tcctgtggtt
    13681 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    13741 acttccccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcggttt
    13801 gctttcgtca attctaccac cgctgtctga cttctttcga tctcaaactc gatcggtgtc
    13861 gcatctaatt catacccc
//
LOCUS       DAXWLF010000066        13766 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXWLF010000066 DAXWLF010000000
VERSION     DAXWLF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.066"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(5..781)
                     /locus_tag="ACGE4U_001459"
     CDS             complement(5..781)
                     /locus_tag="ACGE4U_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFF8393631.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(1029..1865)
                     /locus_tag="ACGE4U_001460"
     CDS             complement(1029..1865)
                     /locus_tag="ACGE4U_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFF8393632.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1876..2805)
                     /locus_tag="ACGE4U_001461"
     CDS             complement(1876..2805)
                     /locus_tag="ACGE4U_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFF8393633.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2997..3350
                     /locus_tag="ACGE4U_001462"
     CDS             2997..3350
                     /locus_tag="ACGE4U_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8393634.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3424..4080)
                     /locus_tag="ACGE4U_001463"
     CDS             complement(3424..4080)
                     /locus_tag="ACGE4U_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFF8393635.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4306..6240
                     /locus_tag="ACGE4U_001464"
     CDS             4306..6240
                     /locus_tag="ACGE4U_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8393636.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6326..7549)
                     /locus_tag="ACGE4U_001465"
     CDS             complement(6326..7549)
                     /locus_tag="ACGE4U_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8393637.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7543..9414)
                     /locus_tag="ACGE4U_001466"
     CDS             complement(7543..9414)
                     /locus_tag="ACGE4U_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFF8393638.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(9435..10553)
                     /locus_tag="ACGE4U_001467"
     CDS             complement(9435..10553)
                     /locus_tag="ACGE4U_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8393639.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10636..11469)
                     /locus_tag="ACGE4U_001468"
     CDS             complement(10636..11469)
                     /locus_tag="ACGE4U_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8393640.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11462..12379)
                     /locus_tag="ACGE4U_001469"
     CDS             complement(11462..12379)
                     /locus_tag="ACGE4U_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8393641.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12402..12581)
                     /locus_tag="ACGE4U_001470"
     CDS             complement(12402..12581)
                     /locus_tag="ACGE4U_001470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393642.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(12606..13730)
                     /locus_tag="ACGE4U_001471"
     CDS             complement(12606..13730)
                     /locus_tag="ACGE4U_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8393643.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4286 a         2744 c         2249 g         4487 t
ORIGIN      
        1 ttctttaaaa tgattcattt tctatctttt cttgcataaa attctttagt ttatcaatac
       61 ttctctttcc catctctagt tgttctccat ttgcaaatgt tactatttcg tttactctgc
      121 taatagatgt aatgttttca gtattaatga tataagcttt taaatcttta cataaatact
      181 catattcgag tgatttttta tattttttaa ttgtgccgtt tacgagaatc tcttcatctt
      241 tcaatactag gcctatttta tttttataac ctggcatgct cattagataa aggatttcat
      301 cgtaatggat atagtgatcg ctattaccat aacggacagt tgtccacctt tttttcgtag
      361 catgttgaac aggatagttt tctttttctt tcaataagta actttggatg ctttttttat
      421 ttttttcata agtgaattct tttatcaaat aaccactagg cgtaatcttt cgattaataa
      481 cagttattgc tttattcgga tgagacgtta gataaacaat tttacacttt tcattctttt
      541 ctctaagcat ttcgcctagt tcgataccgc tataataggc gttcaaatcg atgtctagga
      601 aaaaaatatc tgaggaatag atttcattat ttttaatgaa ttcaacaaat tgtataacgt
      661 tcaatccctg aacgatctca aatttccaag gacttaataa aattctggat tcttccaaaa
      721 tttctactag cattttttca aaaaccaaac tatcatccaa aataaatata cgcgttacca
      781 taaaaagtac ctccactcga tattttacta tcaaaatcat ttcatacaac ttttcaacat
      841 acatgttagg aataaattgt aattctcttc acaatctttt ccaacacctt tgaaaccgat
      901 atcaaatact ttcaggatgc tgcttctaaa ataaatatca aacaaatgaa tacatcatgc
      961 aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt ctgagctatg tttatttatt
     1021 ttgttctttt aaaaagaacg atacacatac tgatttttcg gagcagagac tttctcagcg
     1081 tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt gataatagtc tgattggatg
     1141 gttccttcta ttttatacca ttcatcattt ttaaccgtta ctccttccgg catatgcgtc
     1201 aacaaaccaa agactcctga atctgccaca caatggataa tgccgaatcg gaacaagaag
     1261 atatctgtat tgctatcgtt cggtgtttgg taaacaaatc cttcatagct gatttttttg
     1321 ccaatgaatt cgctagggta attataaatt agctccataa cttctaaata gttttcatct
     1381 gtgatcttga tagtttgctt atccccatac ttttctttca actcattcat ttggtctaag
     1441 tagtctgatt tgttgaaata aatactggta tccggcttta agtattgtgt ctgcatctcc
     1501 ggatccccta ctgattcttt acttactggg aaattaaatc ctttggcttc tacgatcgta
     1561 gtgtcgagac taactgttgg aaacaacgtc cccacgatta ctggcaaagc tagcagtaca
     1621 taagcgattc cacgttgata aggttttgac aacccatggt catgatcgtg ctcgtgtgcc
     1681 ttttcttctg cttttccgtt tttgacccac aaatacagct gaactaaagc caaaagagca
     1741 gataaaatca ttgaaagaat agctaagtag cgataatgga cattgatata ttgatcgagt
     1801 ttgccagaaa cttgcaagta catcatcaat tcgaaatacc cagataaaat caaaaaccgc
     1861 agcatatttt ccctcctaaa taaataatgt gaatccaata acgctaaggg caatcagtcc
     1921 gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc atcatcagta gatttttgat
     1981 atcgaccatt gggccaaaga ccaaaaaggc agctacaggt gctgtgccga acagacttaa
     2041 caaagaagat cctataaacg catctgcttc tgagcaaaga gacatcgtaa aggccaataa
     2101 cagcatgatg ataatggcta aaatctttgt tgagccgagc gttagcataa ttctagttgg
     2161 taaataagtc tgcatcgcag ctgcgattaa tccgccaatg atcaaataac gacctgtatc
     2221 aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt tgatggatca gctgattttt
     2281 tggttcattc ttctcacttt gttcttcttc atggacatga tgatggtgat tttctgctac
     2341 tttagacgtg attttttctt tcaatacagc gtctttttga aaataggcaa gccagacacc
     2401 aatcacaagg gcaacaaaaa agctgcctat cacccgccag cttgccatct gccaagaatt
     2461 gccaaatgcg ataaaagtgg aaaaaatcac gattggattg atgatcggag cagtcagcat
     2521 aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca aattgatgaa cgatcggtac
     2581 gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc gaaccagtaa aaatagagaa
     2641 gatacgattt tcaggaagcc atctcttcac acgttctggg gtcaaaaata cttgtaaggc
     2701 gcctgagata agacagccta ataatacgaa aggtaatgct tcaataacga tcgataagaa
     2761 aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa aacatgagtt tcctcctttt
     2821 tatttctagt tcattcgctt tgcaaatata cgcttttatt caaaaaaaac aagttgctct
     2881 gtaaattttt acacaactct tattttttac caaagcactt caattgactt tttctgttat
     2941 tcccattaaa ttaaggtctc atacatatca cttctatcat aaaagggaga ataaaaatga
     3001 aacaaaccac taacttgact gaagttcaag acatactgca acagtcatcc gttgccgtca
     3061 tttatctttc gatgcctaat tgcagtgttt gtcacgctgt tcgtccacga ctagaggaac
     3121 ttctcacaca ttatgatatt ccggcacttc atttggatgc atttgaaaca ccagaagtag
     3181 ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat ttttcatcaa ggaaaagaag
     3241 tctttcggca agcacggttc atcgacttta aaaaaatccg ttatctacta gatgaactga
     3301 cagcagaaga tgattccctt tcttatgaag aaatcttccg aacaaaataa tcgttgtcca
     3361 aacagaaaaa agcgattgaa caagtgaaag ttttgccttc atttgttcaa tcgcttttta
     3421 tttttattct gctgtatttt cggaatgcgc atttgcgtca gaatgcaaga aatagatcaa
     3481 ggtttgcaat tcattcgtca aatcaacatt ttggatcaat acatctgacg gagcattcaa
     3541 tcgagcagcg gtaaagttca agattccttt gattcctgct tccgttaact gattgactac
     3601 ttcttgtgct ctttcagcgg gtatcgttaa aattgctact tcgatttgtt ggatcttgat
     3661 ttgttctgac atatcttcca ttggataaac ggggataccg tctacgatgc gcccaacgat
     3721 ttcctcattc acatcaaaag cacaactgat acgtatgcta ttactttgat ggaatttgta
     3781 ttttagtaaa gcacttccca agttccctac accgatcagc gcaacatttg tcaattgatc
     3841 gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct acatcataac catacccacg
     3901 ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc gttgcactat ctacttgaac
     3961 ggcttcactt aattcagttg aagacacttt tttctttcca gcattattta aaatccttaa
     4021 atagcgataa tacaaaggca gtcttttagc tgtcgccttt ggaataatat gatctttcac
     4081 gaaatgacct ccacgtattg tctaattaca tctttgattt ctccatcata gcagtattta
     4141 taggaaaaaa tcaattccat tgctcagata ttgtgaaatt ttattcttta ttcatttatt
     4201 tatcatttct gcaaatctcg cagtataatg gaatttatgg tagactacta attgaaaaat
     4261 agttttttat ttgaggagaa ttcacaaagt agaaaagagg attccatgat attattacaa
     4321 gccaatcaga ttgctcgcca ttttggtgca gaagtcttat ttgaaaacat acatttagaa
     4381 atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg gcgctggaaa atcgacttta
     4441 ttaaagatca ttgctggaat tgaagcacct gatgaaggaa agatcgctaa aaataaaaca
     4501 gcgactctcg gatacctcgc tcaggacaca ggacttgatt ccaatgaaac agtttggaac
     4561 gagatgctga aagcttttga acaagttaga caaatggaag agcggatgcg cgagctagag
     4621 attttcatta gcgaaggtac gccagacaca cctgcttata gttcttattt aaaagaatac
     4681 gaccggctgc aacacgattt ttcagatcga aacggctatg gttatgaaaa tgaaattcgt
     4741 tccgtattgc atgggtttaa attcgattct tcattttatg atcagaaaat cgatacttta
     4801 tcaggcggac agcgcacacg tttagcttta gctcgcatgc ttttgcaaca ccctgacatt
     4861 ttgattctag atgaaccaac gaaccattta gatatcgaga cactttcctg gcttgagaat
     4921 tatctgcaaa gctatactgg tgcattattg attgtttccc atgaccgtta ttttttagat
     4981 aaagtagtca atgaagtcta tgaattaagc agaagaaaaa tgcgccatta caaaggtaac
     5041 tattcgaaat atctagattt gaaagcagaa cagttagcaa gtgaatggaa ggcttatgaa
     5101 aagcagcaaa cagaaatcca taaattagaa gacttcgttg ctcggaacct tgttcgcgcc
     5161 tctaccacaa aacgagcgca aagccgcaga aagacattag aaaaaatgga ccggctggat
     5221 cgtcctcaag gggatgaaaa acaagcaaac tttctattca gtatagaaaa gccttctgga
     5281 aatgtagtct tgcaaatcga agatgccgct atcggatatg ataatgtcac cttatctgaa
     5341 ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc tagtaggtcc gaatggtatc
     5401 gggaaatcga cgttgctgaa atcaatcatt caccagatcc cttttatccg tggaaaagaa
     5461 acgctaggga caaatgtcac catcggctat tacgatcaag gtcaagctga tctgcataca
     5521 aacaagacga tcctagcaga attgtgggat gagcatccta ccactccaga aaaagagatc
     5581 cgaagtgctt taggtgcttt cttattttct ggcgaagatg tagaaaaacc aatccctctg
     5641 ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt tagcgatgaa caaagaaaat
     5701 tttttgattt tggatgaacc aacaaaccat ttagatattg acaacaaaga agtattagaa
     5761 aatgcattga tcgattatga cggcacactg ctttttgttt cccatgaccg atatttcatc
     5821 aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg gcagcaaagt ctatctagga
     5881 gactatgatt attatttaga gaaaaagcag gaagaagaag aaatcgccga attgttggca
     5941 caagatgagc cagaatctgc tcctgctccc acgaacaaat ctgattatta ccaaagcaaa
     6001 gaacagcaaa agcttatccg tagccttcaa cgaaaaatta ctcaggttga agaagaaatg
     6061 gcgcggatcg atgaatggat cgatcagttg gaacaagaaa tggtcgcgcc agaaaacctg
     6121 gaagatcacg taaaactcaa tgagctgaac caagatttag aagctgctag acaagatcaa
     6181 gaaacaaaat tgacggaatg ggaagaacta agcttggaat tagaagaata tgaaaattaa
     6241 cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt tttctttttt tattttatca
     6301 aacaaaaatt ccatatttac ctttttcacc aataagaaga ccaattacta gtcattctca
     6361 ctaaagcttc taaataatag taatcacccc aaatacaagg tgtatttacc ccattatttt
     6421 tgggcttatt ataaacagac tgtgttaaaa agccatcttc ttcttccgtt gtagcatatc
     6481 tttctttcaa ttgtcgcata attttccaaa cagcagactg gtataagttc ttttctgttt
     6541 tgtctaatag acctgattct attaattcta ataatccaca aaccatgatc accatactag
     6601 atgtatcttt ttcttctctg ctagaatcat caaagatcaa atcccaataa caaatggtat
     6661 cttctggcaa ccgattcata aaataattgg ctaatatttt gcttaaatca aaatattttt
     6721 gttctttcaa ataacgataa cttaatgcaa aaccgtaaat cccccatgct tggcctctag
     6781 cccaacagga attatcacta taaccttgat gcgtacttcc atatcttggt tttccatctt
     6841 tgacatccat ataaaatgta tgataagttg aaccatcttc tcttactaaa taatctgctg
     6901 cattttgaat atgagcctga gcagcttttt tatatttttg atttccagtt tcttcactcg
     6961 cccaatacaa taatggaaga ttcatgcaac aatccatgat cattctacct tgttgtgaag
     7021 gatcactagg atctccccaa gcttgtataa ctcctgcctt ctcccaaaaa cgctgaagta
     7081 atgtttctgc agcatcaatt gaagcttcct tcatttttcg atttcccgtt attttataat
     7141 ctgctacggt tgataaagaa tacaaaaaac caagatcgtg atgattagtg ataatttgac
     7201 agtcaatcct gtggataaaa tctatatctg ctttttcagc tgtttgttga aataaaggat
     7261 cacccgtctt ctcaaataac aaccaattaa ttcctgtcca aaatccttct gtccaaccaa
     7321 tattgtctct taatggataa cgtcctttta taggacactc gcttgggaat tgatactgac
     7381 tgttactgat acgtttgatt aacctctccg tgtgtctgcg acaacgctcg atctcattat
     7441 tccattcttt ttccattaat tctttataac attcatttat ccatttttct ggatgtatta
     7501 tctcttcaaa catcacttta tcaagcattt ccccaccagc ctttagcata attttagtct
     7561 ttttttattg tttttatttt ggatagttag cccggttgtc ccatcaaaat caattttggg
     7621 acgattgttc catacttcta aactataatc ccgatcttga tgagcaacca ctcctgttcc
     7681 gagccaatgg gttccttttc caaatgtacc ctctaatcca ggaataaaag tgttttccca
     7741 tgtcatcaag tttgtattgg gcaaacccat aatagctgtg gattttcgtt taatagtatc
     7801 gtcacttaaa cagataatac ctgaaaagcc agcttcactc accctatagt tttccttgtc
     7861 ttcttctatg attattcctt ctttctcagg agaacattca atagcaaacc ctgtctcata
     7921 tagaacatat tctgtatcta attctacttt gtgtacgcga atctgccagc cttcaaaagg
     7981 aataagccaa gttctgattt ttgcctgtaa agtaggttcc catgtagagg caagtacctc
     8041 tttcttacat acaatgtctc gtacattatt tcgaacgatg aactgtccat aggttttgat
     8101 gctctcattc ttaaaactgg tcgtgatata tgtgtccgct cgctgaatcc ctagcattga
     8161 atccattgcc atttctgctt tcaattgatt cgctcgcggt atcgaaaaac caaattgact
     8221 agaataagca aattttaaat atttttcatt ggtcaatgta tggaaatttg gccctaattg
     8281 tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt atgagcaaat tggcttctga
     8341 aagaagttta gttccttcac gaataggcat aggcatttct ttagccgtcc aatactcatg
     8401 gtcttcaggt agcgccaaca ataaaaaaat cttattcagc cataatggag ataacgtact
     8461 attatagggt tcagtcaatg tcaattgagg gtaggtataa ccaatattca ggataccttt
     8521 ttcatcaaat atttcttttt gcatccacca tcttatattc cgattgacta accccttgat
     8581 ttcacctaat ttaaaaggca aaagatcggc ccacaacata gctgaccaaa agcttgaaac
     8641 tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt gcaccatctt ccgcaaaaaa
     8701 atgaatatag tctctagaaa aatcaatagc tcggttctta tatatctggc taatgggctt
     8761 ttctggttct agtacgctat aaatcaaacc ataataatga aatgcaaaag gattataata
     8821 atcttggcga cctcttgaac tgtctcgata ccagccattt tgaagataac aattttctat
     8881 attatcaaat gcttctttca gtgctgattc tttaactgga taaccaaatt tttggataat
     8941 tgttccgaca attatcttaa aaaattgcca attaccatct gcacattcat attcaaaaat
     9001 ttgcgagaac caatgaataa tctggctttt ttctcctgta tctaattttt tccataataa
     9061 ttgttcatga tgatataacg ctaataacat tgcgggcatt tctaccatac gctggtctct
     9121 ttcatgtatt tctccccaat aatcgggatg ggaaggatct gttccagctt ttattcctga
     9181 tagaattaat ttcaaccatc gttcatctaa ttggtcaatt gcgggccccg ctccccataa
     9241 aacccgagag aatccttcca ttcctgcaat tttatttcca tatcctaccc cttgatgtat
     9301 ataatcaatt tgtgcgccac ttgctcgata aaacggctgg agtggttgat ttatcttttt
     9361 aaataagtca aagtaatcct gtctggtcct taatagtggt actggatgtt tcattgtatt
     9421 ttctcctctt tttactacca aatagctaat tccgactgtt ctaatcgaag taagccttca
     9481 ataaaaaaat aatcagaata gatcagtggc acattataag tcagttctct atggtatgct
     9541 tctgctccgc ctccaatgat cccatcttca tccatattcc aatttgcata gtgcttttct
     9601 gtcttttgga gtatattgat agcagcttca tgatacattt ttgatcgtac agaggaaaca
     9661 tatcctgcta attccagaag accgcatgca gcgcatactc cagcactggt atcaatattc
     9721 gaatctttta cagcaaaatc aatgagagga atataaccag ttttttcgat tttctgaata
     9781 aaatttactg ctatcttttc tgctatagct agatagtacg gattttttgt gtaacgataa
     9841 cttaacgcga atccatataa agcccatgca tttcctctgc tccaacaaga attctcattg
     9901 tatccttgcc cgccaagtaa ccgaatatat ttccctgatt ctgcatcaaa ttctgcgata
     9961 tgcccggttg atccatcttt cctcactaga tacttagctg ttgtatctgc atgctgccga
    10021 gcaattttag agaacctcgg atcaccagtt tccctcgaag cccaatacag taatggtaaa
    10081 ttcatcatac tatcaataat catccaacca ggtttatggt cattccatgc tactaaatag
    10141 ttccctgaaa tattatagcg tccagcaagt accgtagctg catgtaaccc tagttgtcga
    10201 ctatgtttgt tttgagactg ttgataatgt gcaatagccg atggtaagaa taaaaaacct
    10261 acatcatgat gaacgtctga aaaatggtac agggcagatt ctaataattg ttcttgttgt
    10321 attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa agagctgcca taaaatacca
    10381 ctccaaaaac cattggtcca ccagtcgatt cctctgtcct ttattacgtc tgtatatttt
    10441 ccgttgtaag aaatatatgg aattttattc cccattctgt ctaattccgc atctatcttt
    10501 tcagaagcta cattcaaaat ttttcttgac cattccttcg tctgttcatt catggatatc
    10561 ccgccattct tttaattttt atgaatgtgg agttgctcct agaacgaact agatggttaa
    10621 cattcgttat tttttttact tatttgcagc tccaatgtca ttctccttaa atttagactg
    10681 cgcccacaca taaatcaaga ccattgggat cactgaagtt gctaaaatta gcataattgc
    10741 gccataatta attggctcac ctgtactaga aaccgtcata tttttgataa ttaagggaac
    10801 cgtatattta tctttatctg aaagcatgac taatggaaat agatattgat tccaacttcc
    10861 cataaagata agtaaaccac tagtaataat cgtaggtgat aaaatcggta cagcgatttt
    10921 cgtaaaaatc acccactcat tggcaccatc gattctggca gcctccatca gtgctgtagg
    10981 aaattgactg ctatattgtc gcatcataaa cactgaagtt gtactggcaa tcgaagggat
    11041 gatcacacct gcataagtat tcgataatcc taaacgactc ataatgtaaa ataaaggaat
    11101 caatgtggta aaaaaaggca ccatcctaga tagcataact gtattgaaca acagtttatt
    11161 ccccataaaa cgatactttg ctaatgcata acccgccatt gtagaactgg ctgtagctaa
    11221 aactgtactg attacagcaa taacaatcga attccaaata atcaagggat aattatatct
    11281 cgccatgatg gcttgccagt tttttaatag catatcccca aacttgaaat tgattccgcc
    11341 acttaaaatt tcttgattac tttgcgttga agctacaatc atatataaat atggcatcaa
    11401 aaatattaag gcaccaaaca caataatgac gtacgtaaaa attctgcttt gtatcttttg
    11461 tttagtcatt tttatcacct ccgaatttaa attgaatagc cgataatata acagatacaa
    11521 acaccatcgt ccaggcaata gcagaggctt ttccaaaatc aaccatttca aaacttgttt
    11581 tatataaatt gactcctacg accaaagctg aattttctgg tccattggaa gtcccaaaga
    11641 atatatttgg gatattgaaa ttacccaagc cattgatagt ggctaaaact gtggccatta
    11701 aaatgattgg ctttagcatg gggatcgtta ataaaaagaa ttgccgaaat ttcgatgctc
    11761 catctatttc agcactttcg tatatctctt tagaaatccc ttgaagacca gctaaaaata
    11821 aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat gatcattgat attcgtatcc
    11881 aaaaaggatt tgttaaccac tgtacaccgt taattcctag cgcattcaaa aatccattga
    11941 ataacccagt cggattgaaa aaagtcatga atacggaagc tacagccacc gttgatgtaa
    12001 tggttggaat ataatagaca gttctaaaaa atccttttcc ttttaactgt gctgaattca
    12061 ataaaacagc aaagtaaata gctaatagca tcgttattgg aattgatcct gccatgataa
    12121 tcaaaacatt cgtgtaagat ttaataaaca atggatctcg gaagatagct agataattgt
    12181 caaatccaac aaattcaaat gagtagccaa ttttcataaa actaaaagca aacgagataa
    12241 tgatgggaat caaaatcgtc acaataaaga aaataacaaa aggtgtgacc aatccataag
    12301 cacttttttt atgagacgta tctgttgctt ttttcttgct gttctttatt gtcttgcttg
    12361 gtaacgaaga ttgtatcatc gttaagtcct cctaatattt tttaatcacc agttaatttt
    12421 acgccgtact tatgagcaaa attttctgct tcttttagat atgaattttc aaaatcacct
    12481 gtttgccaat agttatatgc agcggtagtc aaataagacg tagcatctga ataagtggct
    12541 ggtaaaacag tatttgatgt attttcattg attttattca atagttcata gtatttttgt
    12601 cctccttagt agtcaaactt tttctcagct gcttctgttt gataggccaa tgagttagat
    12661 gtagccacgc ccagctccag tgcgttggct aagcattctt catctgtcag cataaatgta
    12721 agaaattgtt gtgccacttg tggatttttg gcatctttag ggacataaaa acttgatcct
    12781 cctgatactg caccttttcc tggattttct tttgaaaaag gaatcagtgg agccatgcgc
    12841 catttgtcag atgcatcagg aaaattcaaa gacatattcg tagcatacca gccgccagca
    12901 acaaacattg cagattcttg aaatgctttc tcagcatctt gtgcaccgta gaaatgtaca
    12961 atatctgagt cgatcatttc tttgattaac tttgcagcat gtttcgcttc tttagtattc
    13021 agacggatat ttccttcttc atcaagcaaa gattgattct gctgggctag catgttgata
    13081 aacaattctt ggtccccaga ttctggcaag gcaattccaa aactaccagt tttttctttt
    13141 aatgcttttg ccacctggat atattcttcc caagaactga tttcttcaaa atctacacct
    13201 atttcagcaa atttttttgg ttgataataa caaagtgctg gggtaaaatc actagcccaa
    13261 ggaataatcg tttcattact cgtttgttga cgcaaaatat tcaatttttg atcataaaac
    13321 tgatcgccat atttgtcata aaatccatat tcatcagcac tataaaaagc atcttgaaat
    13381 ttttccagca cacctgctgc tgaagtgtct gccatggaga taatatccgg catatcttcg
    13441 tgtgttacca attttggggt gatcatttgt actgttgctg ttcccgaagc tacattcaca
    13501 atttttaagt caatatttgc tccagttttg cggttataga tttttgcagc attttcaaca
    13561 tagtttgtca gtttgccgtt tgcgacatat acttctaatg tagtggcttt ttttatcatt
    13621 ggatctgcga tgtttgctgt tacttcttcg ttagtttttc cacatgcact taagctgaat
    13681 aatagggtaa tgtttagcaa agctatcacc aatttgtatc tttttttcat gtctatccct
    13741 tctttctttt caagttctta tataaa
//
LOCUS       DAXWLF010000067        13687 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXWLF010000067 DAXWLF010000000
VERSION     DAXWLF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.067"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(32..3685)
                     /gene="rpoC"
                     /locus_tag="ACGE4U_001472"
     CDS             complement(32..3685)
                     /gene="rpoC"
                     /locus_tag="ACGE4U_001472"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFF8393644.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3764..7381)
                     /gene="rpoB"
                     /locus_tag="ACGE4U_001473"
     CDS             complement(3764..7381)
                     /gene="rpoB"
                     /locus_tag="ACGE4U_001473"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFF8393645.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8109..9077
                     /locus_tag="ACGE4U_001474"
     CDS             8109..9077
                     /locus_tag="ACGE4U_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8393646.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9095..10000
                     /locus_tag="ACGE4U_001475"
     CDS             9095..10000
                     /locus_tag="ACGE4U_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8393647.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9997..10803
                     /locus_tag="ACGE4U_001476"
     CDS             9997..10803
                     /locus_tag="ACGE4U_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393648.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10960..11928
                     /locus_tag="ACGE4U_001477"
     CDS             10960..11928
                     /locus_tag="ACGE4U_001477"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFF8393649.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12245..12769)
                     /locus_tag="ACGE4U_001478"
     CDS             complement(12245..12769)
                     /locus_tag="ACGE4U_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFF8393650.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13056..>13687
                     /gene="scm"
                     /locus_tag="ACGE4U_001479"
     CDS             13056..>13687
                     /gene="scm"
                     /locus_tag="ACGE4U_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFF8393651.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFL"
BASE COUNT         3843 a         2961 c         2709 g         4174 t
ORIGIN      
        1 ataatttgtc acaacctctt ttttaaaata tctattttgt ttgattgttc attgcgtctt
       61 ctgcagccat ttgcgcttca atatctgaaa tactgtagac attttcgctt gctacaccga
      121 cttcttttgg ttccatgtca cgataacgtt gcataccagt accagctggg atgatcttac
      181 ctataataac attttctttc aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat
      241 ccgtcaagac acgagttgtt tcttggaatg atgcagcaga taagaaacta tttgtttcta
      301 atgatgcttt tgtgatacct agtaagacag gtcgagaagt tgcaggaacg cctcctgaga
      361 ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc taataatgta cctggtaaga
      421 tatctgtatc acctggatcc attacacgaa ctttacgcag catttgacga accattactt
      481 cgatatgctt gtcgccgatt tctacccctt gcatacggta aacacgttgt acttcacgaa
      541 gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa caattgtttt ggatcgattg
      601 atccttctgt caatggtgca ccacggtgga tcatatcgcc ttctgctact ttcatacgtg
      661 ctgtataagg aacagtatac gtacgtgtat ctgtttttcc tttgattgtt acttctttgg
      721 tacgtgttgc aggatcttct gagatatcga tgacatcccc tgttacttct gtgatcactg
      781 cttgcccttt cggattacgt gcttcaaaga tttcttggac acgaggaaga ccttgagtga
      841 tatcgtctcc ggcaacccca cctgtatgga atgtacgcat cgtcaactga gtaccaggtt
      901 caccgattga ttgagcggcg atcgtaccaa ctgcttctcc gacttcaacg tcagagccag
      961 ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg ttttgtatta catgtgaaga
     1021 cagaacggat tgtcactgtt tcgacacctg catcaacgat ttgttttgcg atgtcttcgg
     1081 aaatcaattg gtttcctgca atgatcaatt catttgtttc aggatgacgt acttctttac
     1141 gggtgtaacg tcccactaaa cgatcttcta atgattcgat gatttcattt ccctcgcgga
     1201 tggcttggat ttcaagacct cggtcagtac cgcaatcttc ttcacggatg attacatctt
     1261 gggccacgtc aaccagacga cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat
     1321 cggtcatccc tttacgagcc ccgtgagttg agataaacat ttccaagacg gatagtcctt
     1381 cacggaagtt cgaaatgatt ggcaattcca tgattcgacc attaggagcg gccatcaaac
     1441 cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc acgagctcca gaatcagaca
     1501 tcatgaagat tggatttttc gcttccaaac cttccatcag tttttgttgg atgctgtctt
     1561 ttgcagcatt ccaaacagca atgacacgtt cataacgttc gtcatctgtg atcagaccac
     1621 gacggaattg ttttgtaata gtttcaactt gtttatgggc attttcaatg atttcttgtt
     1681 tttcattcag tacactgatg tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt
     1741 tgtaccctaa gtctttcata cggtcaagca tcttagaagt ttctgttact ttgaatcgtt
     1801 tgaagacttc tgcgatgatg tttcctaagt ttttcttctt gaacggtaat actaattctt
     1861 gctctttgat atgtgcagga atatcagtac cagcttcaac aaagtatttg tccggtgttt
     1921 ggacggtcaa gttgaagtct gttggttcat tcagataagg gaattcagga ggcatgatct
     1981 cgttgaagat gatcttacca actgttgtga tcatcgtacg ttctttttgc cattcagtaa
     2041 atggtttttc acctaatgag ttagggttca caccgatacg tgaatgtaaa tgaacatatc
     2101 cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa gaccattcct tcgccttcgc
     2161 gtccttcttc ttccattgtc aagtagtagt tacctagaac catatcttga gaaggagtaa
     2221 caactggttt accatcttta gggttcaaga tgttttgagc agctaacatc aacatacgtg
     2281 cttctgcttg tgcttcttca ttcaatggaa cgtggacagc catttggtct ccatcgaaat
     2341 cggcattata agcttcacat acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa
     2401 ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg tgcccggttc agcaatactg
     2461 ggtgttcctt gattacgtct tctaaaacat cccatacttc gtcttcttga cgttcgattt
     2521 tacgtttcgc atttttgatg tttgaagcta gttcgcgttg aacaagctca cgcatcacga
     2581 atggtttgaa taattcaatg gccatttctt ttggaagtcc acattggtac atcttcaaga
     2641 aaggtccaac aacgataacg gaacgacctg agtagtcgac acgtttacct agcaagtttt
     2701 gacggaaacg accttgtttc cctttcaaca tatgagaaag agatttcaat gggcggttac
     2761 ctggtccagt aactggacgt ccgcgacgac cgttatcgat caatgcatct actgcttctt
     2821 gcagcatacg tttttcattt tgaacgatga ttccaggtgc attcaagtct aacaaacgtt
     2881 tcaaacggtt attacggttg attacacgac ggtaaagatc attcaagtca cttgttgcaa
     2941 aacgtccacc ttctaattga accattgggc gtaaatctgg tggaataaca gggataacgt
     3001 ccataaccat ccagcttggt tgatttcctg aagcgcggaa tgcttctaaa atatccaaac
     3061 ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt caattcttct ttcaattcgg
     3121 ctgcttctgc atctagatca acattatcaa gtaattgttt aatcgcttct gcacccatag
     3181 cagcttggaa ttcttgtcca tattgttcac gtttttcgcg atattcgcgt tctgtcaaca
     3241 attgtttttt ctctaaattc gtatttcctg gttcgatgac tacatatgaa gcaaaataaa
     3301 tgatttcttc caaagcgcgt gggctcatgt ctaatacaag acccatacgt gaaggaatcc
     3361 ctttaaagta ccagatatgt gaaacaggag ctgctaattc aatatgtccc atgcgttcac
     3421 ggcgaacttt cgaacgagta acttccactc cgcatcgatc acaaacgatt cctttataac
     3481 ggatacgttt gtattttccg caggcacatt cccagtcttt tgtaggacca aaaatccgct
     3541 cacagaacaa cccttcgcgt tcaggtttta aggtacgata gttaatcgtc tcaggttttt
     3601 ttacttcacc gtaagaccag cttctgattt tttcgggaga agccaaacct atttgcatac
     3661 tttcgaattt atttacatcg atcaaaaggg gttccctcca tttcatttaa tggcaaaccg
     3721 caagacggct gatcttcgac cgccttgcag ctcatagacc ggattagtct aaattatctt
     3781 ctgcggtttc aaagttttgg atgacatcgt ctttttcatc ttccacttgt tcagcagctt
     3841 tcttttctaa ttctttggca gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga
     3901 tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat ctctgtttct tcgatatcca
     3961 agacacgcat gtccagacct agtgattgca attctttcac caatacgcgg aatgattccg
     4021 gtacacctgg ttttggaatt ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc
     4081 cgactacgtc atctgatttg tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag
     4141 cttccagtgc ccatacttcc atttcaccaa aacgttgtcc accaaattga gctttacctc
     4201 ccaatggttg ttgtgtaacc aatgagtaag gtccgattga acgtgcatgc aatttgtcat
     4261 cgaccatgtg agcaagtttg atcatgtaca tcacaccgac agacacacgt ccatcaaatg
     4321 gttcacctgt acgtccgtcg tacagaacag ttttcgcatc gctggccata ccggcttcac
     4381 gaactgtttc ccatacgtct tcatcacttg caccatcaaa tactggcgtt gctacgtgga
     4441 tacctaactg acgtgcagcc atacccaagt gtaattccaa tacttgcccg atgttcatac
     4501 gtgaaggcac ccctaaagga ttcagcatga tatcgattgg tgttccatca ggtaggaatg
     4561 gcatatcttc ttccggcata atacgggata caaccccttt atttccgtga cgaccggcca
     4621 ttttatcccc ttcatggatt ttacgttttt gaacgatata aacacgaact aacatgttta
     4681 cgcctggtga caattcatct ccagcttcac gagtaaagat tttcacatcg tgaacgatac
     4741 cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa
     4801 agatcgcatg caatagacgt tcttctgcag ataattctgt tacccctttt ggcgtgactt
     4861 tccctacaag caaatcgcca tcttggactt ctgcaccaat acggatgata cccatttcgt
     4921 ctaaatcttt taatgcgtct tccccgacat tagggatttc gcgtgtgatt tcttcaggtc
     4981 ctaattttgt atcgcgggct tctgattcat attcttcaat atggatagaa gtgtatacgt
     5041 catcttttac caaacgacgg ctcatgatga tcgcatcttc atagttgtac ccttcccaag
     5101 tcatgaaacc aactaaaacg ttttggccta gagccatttc gccttgttcc atagatggtc
     5161 cgtctgccag agtatcccct ttttcgactt tctcacccaa atgaacgatt ggacgttggt
     5221 tgtagcttgt tccggagttt gaacgacgga attttgttac atcgtattta tctaatgcac
     5281 cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta ttcaacgaca ccgtcatgtt
     5341 tacataacaa agcagctcct gagtcatgcg ctgatttgta ttccatacca gtaccaaccc
     5401 atggtgcttg aggattgatc aacggcactg cttgacgctg catgttggca cccatcaagg
     5461 cacggttgga gtcatcgttt tccaagaaag gaatacatgc tgtcgcaacg gcaactactt
     5521 gtttaggtga tacgtccatg tagtctactt tatcaataga tacttctaag ttttcacttt
     5581 gagcacgagc cataacaacg tcttgagcaa atgttccatc ttcattcaat gggctgtttg
     5641 cttgggctac gatgtaatgg tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa
     5701 cacgacctgt ttcacggtct acacgacgat aaggtgtttc gataaatccg aatttattta
     5761 ctttcgcata actggacaaa ctattgatca acccgatgtt tgggccttca ggagtttcga
     5821 tcggacacat acggccatag tgggagtagt gaacgtcacg aacttcataa ccggcgcggt
     5881 cacgagtcaa tccgccaggt cctaaggctg aaagacgacg tttgtgtgtc aattcgccaa
     5941 gagggtttgt ttgatccatg aactgtgaca actgagaaga accaaagaat tctttgatac
     6001 ttgccacaac tggacggata ttgatcaatt gttgaggtgt caatgtttca gtatcttgga
     6061 tcgacattct ttcacgaacc acacgttcca tacgtgctag cccgatacgg aattggtttt
     6121 gtaaaagttc accgactgaa cggatacgac gatttcctaa gtggtcgata tcatcgacat
     6181 tgccgatacc ttccatcaag ttcaagaaat agctcattga agcaatgatg tcggctggac
     6241 gaaccgtttt tacagctgct tctggatagc cgtttccgat cacgtttact tcacgttcag
     6301 ggtctcttgg tgaaaatact ttgatgactt ggacagtcat tggatctgtg accacaccgt
     6361 cttctgatgg atagtaagtc actgaattta ggccattatc aatgaaagga gctagtgttt
     6421 ccatgacttg gtgagtcaga actgttcctt tttcaacgat gatctcgcct gtttctggat
     6481 caactaatgt ttcagctaat gtcagattca atagacgtgt tttcaaatct aattttttgt
     6541 ttactttgta acgaccaacg tttgctaagt cgtaacgttt tggatcgaag aaacgagcat
     6601 tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa
     6661 cgtctttcaa accttcttct gttcttgagt cgcttgcgtt tttatgcaag tctttttcga
     6721 ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa
     6781 atcctaacgc acgaactaat acggttaaag gaatcttacg tgtacggtcg atacgtacat
     6841 aagagatgtc ttttgcatca gtttccattt ctagccaagc tccacggtta gggataacag
     6901 tagaaccgaa tccttctttt ccgtttttgt cgacttttcc atggaaatag acacctggtg
     6961 aacggactaa ctgagaaacg attactcgct ctgccccgtt gatgatgaat gttccttgtt
     7021 ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc
     7081 ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta gtttgcatca tgcgcacgag
     7141 cttctgctac tgtatacttt ggttctttca attcataatc tacaaattcc aaagatagat
     7201 tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta
     7261 agaaccattg gtaagagtct gtttgaattt cgatcaaatt tggtaattcc aacacttcac
     7321 tgattcttgc gaaacttcta cgttcgcgat gttttccgta ttttactacg tgtccagcca
     7381 agctcttcac ccctctaaaa tgtcattcaa aaagactacc cgatcaattg ttacaataat
     7441 atttcaaata ttatttttag gtaacaacag acattttttg cggcttttag gcaaaaaaaa
     7501 accaaaaata gaacattctc ttgaaacctt acttcgtgag cttctacttt tgtttcctta
     7561 tattaaagcc gaaacggaca atatttcgtt tctgcctttt cttcatcaga tagtttttgc
     7621 aacttcatat actacactta ttttctataa aagtcaacac ttcctggcgt ttcttctgct
     7681 ttttcgcaac attttcttaa tgttttcaat tttatgtttt caattatctg acaattctta
     7741 attgacagac ctttttatct cggttatact ttagaaaatt cattttgtgg aggaacttct
     7801 atgcgaacca acatcaacca aatcgcacca acacaactga ataactttta ctttagctac
     7861 tttagatagg tttgtattca tcatgatgga tacaaacgtt aaacgacagt ttgtatctat
     7921 gatggctaaa gaactttgta ttgcagcaaa aagccacgta gacaaaatgc taagtggctg
     7981 agcagtgaaa atacaaagta aaagtatcaa gcattctact ttttcaattt attttccgac
     8041 ttttcaatcc atttggacat tttgtctgaa tggatttttt gtttcccaaa attaaggagg
     8101 aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc tgccctgcta ctagctttag
     8161 gaggttgcgc acaaaaagac aattccgcag aggcatcaga tggacaaaaa acaattggcg
     8221 tattgcaact tgtacaacat aactcgttag atgctgccta taaaggattc aaagaaggat
     8281 tagcagaaga agggtataaa gaaggcgata atctaacaat cgattatcaa aatgcccaaa
     8341 acaatcaaga caacctgaaa agtatgagtg agaaactcgt caaagaagat cctgatttgc
     8401 tacttggaat cgccactcca gcagcacaat ctttattaaa tgaaacaacc actatcccga
     8461 tgactgtcac agcggtcaca gacttggaac aagcccattt agtcacatcc aatgaaaaac
     8521 ctggcggcaa tgtgactggc acgacagata tggtgccaat cgaaaaacaa atcggcttac
     8581 tgttaaatat cgtgccagac gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa
     8641 actctaagat ccaagctgat ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca
     8701 aagtactgac agcaaattcc acaaatgatg tccaacaagt gacgaaaagt ctagctaaag
     8761 atgtagatgg aatctatatt cctactgata acacgttcgc aagtgcggca agtgttgtcg
     8821 gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg cgcagttgat caagtcaaag
     8881 acggcggtct agcaacagta ggaatcgact acgaaagctt gggcaaacaa acagggatca
     8941 tggcagcaaa aatcttagac ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg
     9001 atttggaact gtatgtcaat aaagacatgg ccaaagcatt aggcatcaat ccagacagca
     9061 tcaaagaagc tgaataaaac acaggaggat tctcatgaca aatttgctct ctcttttctt
     9121 atcatctgca tcgcaagggg tcttatgggc cctacttgcg attggtgtat acgtcacttt
     9181 ccgtatcttg gacattgccg acttgactgc tgaaggaagc tttcctttag gtgcagggat
     9241 cgcggcagta agtattacaa atggctatca tccattgaca gcttgtcttc taggcttttt
     9301 agggggtgcg gcagcagggc ttgtttccgg actgcttcac acaaaattga aaattcccgc
     9361 ccttcttgca gggatcatca ccatgactgg tttatattct gtcacatccc gtgtgatggg
     9421 tgcgccgaac atctctttat tgggtcagcc aacgatcttt cattgggtcg agtcattcgg
     9481 cataacaaaa gacaacgctg tactgatcgt cggtctgctt tttgctttga tcgttgtatg
     9541 tttacttgtt ttgttcttac gtacagaaac aggacttgct attcgtgcaa ctggcgataa
     9601 tctggcaatg agcgaagcaa acgggatcaa cacagataat atgaagatca tcggttatat
     9661 gatttctaat ggatgcattg ctctttctgg ctccttactt gcgcaaaaca acggctttgc
     9721 tgatttaaat tctggtattg gaacgatcgt aattggactt gcttctatta tcatcgcaga
     9781 agttttgtta cgtaaccagc cactatttct ccgtctcttg actgtcgttt taggtgcggt
     9841 catttatcgt tttattttag cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa
     9901 acttgcttct gctttcgtgc tagtgatctg tctttctttc ccgcagatcc agcaaaaatt
     9961 ccatttacct caaaaaaaga aaggcggtag tgtcaaatga atgacgtctt aacattggaa
    10021 aatctccatc atacctttga aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt
    10081 aatctttcct tagatcccgg cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa
    10141 tctactttgc tgaacagcat cgccggtaca ttttctgtcg atcagggtca aattttattg
    10201 aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta
    10261 ttccaagatc ctaaactagg gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt
    10321 gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca
    10381 tttttcaaag accagctggt tcggctaaat ttggggttgg aaaatcgttt atcctcagaa
    10441 atcggtttgc tgtctggagg acaacgtcaa gctattacgt tattgatggc cacattacag
    10501 cgtccagaac tgatcttatt agatgaacac actgcagcgc ttgaccctgc tacttccaaa
    10561 acggtgatgg aattgacaga aaagctgatc actgagcaga atctcactgc ttttatggtc
    10621 actcatgaca tggaagacgc gatccgctat gggagtcgac tgatcatgct gcatcaagga
    10681 aggatcgtcg tagatatcca gcaagaggaa aaacagcagc tcacagtgcc ccaattatta
    10741 gcgttgttca aacaaaacag cggcacggca ttaaaagacg atcaagtact tttatcttca
    10801 taatcagaaa gaggatggga caaaaaatga acgcccaccc tcttcttttt tctctttcct
    10861 tctttcgttt ttgctttatg tatcaaccgt tgtttcttcg aaaactattt tgttctctcc
    10921 ttcttcctga tagaatggat gggaaaggag cttgatgcca tgacaacgaa agacaaggta
    10981 cttcaattat tgaaaaagac accagatttt ctttccggag aaaagttggc acagcaactg
    11041 gaagtttccc ggacaagtat ttggaaagcc atcaaagaat tggaaaaggc aggctatcgt
    11101 tttgagcacg ggcctacagg gtatcgttac ctgccctctg acgtactaga tgcaaaagaa
    11161 atccagcaag agctacggtc gtttattcct gatttgaaag tacaaacgat gttgacttct
    11221 gaatctacga tgaaagatgc caaactggca gcagctcaag gagattctgt ttcgaagtta
    11281 tttgttgctg atacacaaga acaagctcgt ggacgttttg gacggccttt tttcgctgaa
    11341 cctggacgag gaatctacat gagtctcttg ctccatccag acaaacggtt tgatgaattg
    11401 cctcaatata cgatcatcgc agccagtgcc tgtgtgaaag ccattgaatc tgtaaccggt
    11461 cgttctgctt cgatcaaatg ggtcaatgat atctacatgg atggaaaaaa agtctgtggg
    11521 attttatctg aagcagtcag tgatatggaa agcggcagga tcagtcatat catcattggg
    11581 atcgggctta atttttctgt tcctcaaaat cattttccag ctgatctcca agaaaaagcc
    11641 atctctgtct ttcccgacgg acaagctagt gtcacaagga atcagctaat cagcaaaatc
    11701 tggcaagaat tgttcactta tctttctcac ccagaaaaag cgttggactt ctataaagaa
    11761 aaatcattcg tattgggaaa aaccgtgtcg ttcaatcaaa aaggaacgac ttatgtcggt
    11821 cttgcaaaag aaatcggtga tcatggcgaa ctgatcgtcc aaacaaatga acagaagatg
    11881 agtttgtctt ctggcgaaat cagtctcaca tctatccgta cgcaataatt atcagatttc
    11941 acaaaaggac acctgatctc atacagtgtc cttttttctg aattctcttc aaaagactct
    12001 ccttattcta tcagctgaca gtttctctat cctctagaaa aaggtattct aaaaatatct
    12061 ataaagcatg gtacactttc tttaaatgaa tcaatgcaaa aaacaagaga gtcatcagtt
    12121 tacacgatca aaagagatag caggccgctt cctttttcaa tcttatagat agaccatggg
    12181 taacgataaa aacaaaaaag aacttgcgac ttattttgcc gtaagttctt ttttagctag
    12241 tgccttaatc tcttaatccc aacaaacgtc tcgcatagat cacctgacca gagtgcatag
    12301 cagcatcatc aataatagaa acgagacgca ttcctcgtgt gactggtggt gtccagttct
    12361 cgtcaattac ttcatctagc tcggaaggtt ccagccttct taagtaagaa atagcaaaat
    12421 cagcagctcc tcgcaagtat cctagcagat cttctatttg attagcttgg atctgctttg
    12481 cttgttccag actatgcttc caatcttgtt cgttatctgc tatcgtcatc ggaaaccggc
    12541 tttcccagtc ttgactatgc caaattggag aagttcctgc taaatcagca atctggaaat
    12601 ctaattctcg agcggtatgc caagccagcc aagtcattga tttgatctgc ggggctctat
    12661 cttccactgg aaaacgattg gcctcctcaa tagagacgcc ttcaaacaaa cgttcaaacc
    12721 gatcgtatgc ccgatccaca ctctcgcata gcatatctaa ataatccatt tattttcctc
    12781 ctttttattt atcctactta ctttttccta attctaggat acctacttat atgttactac
    12841 acacccttat tttttacact ttaacagtca ccatcatcaa tagtttggag attgaaagaa
    12901 aaagatagtg tcgaactatc aattgaacaa gaagcaacaa tatgaacgtt tcattaatat
    12961 tctttaaata aacaatgatt tttcaaacga acagtttaag actaggagaa aaagagtata
    13021 ataggaaaat gtttgaaagg aaaggaaggg gaaaaatgaa aaaaagggca agtttactag
    13081 tcctagttgc tttgttgttc aatatctttt cgtatggtgt ggccgtttca gcggaaacat
    13141 acattcctaa agaccctggg aatatttata atgatctaac tggtaactat ggcttgttag
    13201 gaattgctag tcaattccac gtatttgcta aaaataagac aactgtgaat gcacatacag
    13261 acggaaatat agcaacacat gaacttgatg caaaaaataa ttttggtaca gagcttcatg
    13321 aaggtttatt gaaccaagaa ataaattata tacaaaaaac tgattctttc gctaattctt
    13381 caggcatacc tacaggtagc acaagaatga ataaatttgt cgttggcagt aacgtggatg
    13441 tagcacttca agatggtcat cctacagtca atggatccag gcttgatcac ttaactccag
    13501 atgattttta taaagatcgt gctgggcatg aatatattga tttcgagcag gaatttgcta
    13561 aattgaatgt agcagcaaat gatcttgcaa cgatcactcc agctaaaaca tatactgctg
    13621 cagattttcc agacatgaac aatcggacaa tcgatttaac tgatttatca gataccgggt
    13681 ttttatt
//
LOCUS       DAXWLF010000068        13189 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXWLF010000068 DAXWLF010000000
VERSION     DAXWLF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.068"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(334..696)
                     /locus_tag="ACGE4U_001480"
     CDS             complement(334..696)
                     /locus_tag="ACGE4U_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393652.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="ACGE4U_001481"
     CDS             complement(770..1150)
                     /locus_tag="ACGE4U_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFF8393653.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1232..2407
                     /locus_tag="ACGE4U_001482"
     CDS             1232..2407
                     /locus_tag="ACGE4U_001482"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFF8393654.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2484..3068)
                     /locus_tag="ACGE4U_001483"
     CDS             complement(2484..3068)
                     /locus_tag="ACGE4U_001483"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8393655.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3269..4267
                     /locus_tag="ACGE4U_001484"
     CDS             3269..4267
                     /locus_tag="ACGE4U_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8393656.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4379..4888
                     /locus_tag="ACGE4U_001485"
     CDS             4379..4888
                     /locus_tag="ACGE4U_001485"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFF8393657.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4918..5577)
                     /locus_tag="ACGE4U_001486"
     CDS             complement(4918..5577)
                     /locus_tag="ACGE4U_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFF8393658.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5579..6343)
                     /locus_tag="ACGE4U_001487"
     CDS             complement(5579..6343)
                     /locus_tag="ACGE4U_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFF8393659.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6351..7028)
                     /locus_tag="ACGE4U_001488"
     CDS             complement(6351..7028)
                     /locus_tag="ACGE4U_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFF8393660.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7065..8450)
                     /locus_tag="ACGE4U_001489"
     CDS             complement(7065..8450)
                     /locus_tag="ACGE4U_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFF8393661.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8419..8673)
                     /locus_tag="ACGE4U_001490"
     CDS             complement(8419..8673)
                     /locus_tag="ACGE4U_001490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393662.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(8660..8744)
                     /gene="ffs"
                     /locus_tag="ACGE4U_001491"
     ncRNA           complement(8660..8744)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACGE4U_001491"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="ACGE4U_001492"
     CDS             complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="ACGE4U_001492"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFF8393663.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9389..10246
                     /locus_tag="ACGE4U_001493"
     CDS             9389..10246
                     /locus_tag="ACGE4U_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8393664.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10327..10662)
                     /locus_tag="ACGE4U_001494"
     CDS             complement(10327..10662)
                     /locus_tag="ACGE4U_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393665.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10729..12099)
                     /locus_tag="ACGE4U_001495"
     CDS             complement(10729..12099)
                     /locus_tag="ACGE4U_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFF8393666.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12177..12713)
                     /locus_tag="ACGE4U_001496"
     CDS             complement(12177..12713)
                     /locus_tag="ACGE4U_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8393667.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12959..>13189
                     /locus_tag="ACGE4U_001497"
     CDS             12959..>13189
                     /locus_tag="ACGE4U_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8393668.1"
                     /translation="MTFHSQFLSNYIQKQNKKEVFMLIEARKKELLVLQMVIQKNNCS
                     LQELSNDLHIPKRTIKELIRKINVSLKQFLLID"
BASE COUNT         3994 a         2651 c         2173 g         4371 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tgtttcctcc taatatttgc atacttcata ataataataa cataatacct
     3121 aacctctcgt aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta
     3181 taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct
     3241 gctatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc
     3301 aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat
     3361 cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat
     3421 ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa
     3481 acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg
     3541 tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat
     3601 tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc
     3661 atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa
     3721 agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat
     3781 tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt
     3841 atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc
     3901 aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat
     3961 tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct
     4021 ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac
     4081 ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact
     4141 gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa
     4201 tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc
     4261 tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt
     4321 tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat
     4381 ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt
     4441 aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt
     4501 gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa
     4561 cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc
     4621 gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt
     4681 gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt
     4741 gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt
     4801 agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc
     4861 agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta
     4921 gacgagttct ttcttttttg tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc
     4981 aaaaaataat tctaataata caaaaaataa gataaagcta tgcatgaaaa attcttctgg
     5041 aagtacattg atgattggat cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa
     5101 cgtttcatga aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac
     5161 aactggcagt aacatgccga tttgaaacgg acgaatcaac cgccaaagcc gatcattctt
     5221 ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca cagcataatc
     5281 cactaaaaac aaaattttca cttcataaaa gtgatgggct ccgctagcag aaactgggaa
     5341 atctggcaag ctgaggatgg ttttgaaggg attattgaga taattcatta ggtgatcaaa
     5401 atttttcagt aacgtctctt gatctaaaga agtataatcc aaaatttgca aatgtccgat
     5461 atcaaatacg tatagtggtc gaaagttgat cgtcacacca actgccagac tgatcagtgt
     5521 cagaaatagg ctgaacaatc cagcatattc catccactgc catctcgaat ttttcatatt
     5581 agaagctcca ttcatccagt gattgtttca cataggtagc tgggataggc aacccaggta
     5641 catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt atctatatta ttgcgtatcc
     5701 cagcttggat atctgtttca tagttatctc caaccatgat tacttcttct ttttgcagcc
     5761 ctaatacttc aacggcttta tccataatga tggcttctgg ttttccgatg tagatcggcg
     5821 gcgtttgggt tgctgtctct acaaaagaga tgactgaccc tgctccagga agaaggccgc
     5881 gttcagtagg tatattctta tctgggttcg ttccaatgaa agtagcacct ttttgaatag
     5941 caagtgttgc tttaactact ttttcatagg tcagataatt atctaagcct accactacat
     6001 agtcaggagc ctcttcttcc catgtaaatc cggcagctaa aatcaagtca atcaatcctg
     6061 cttctccgat aacatagact tttttccctt ttccatctgc tttcataaaa tcgattgttg
     6121 ccaaagtagc tgtgtagact gtctctggag ctacatgaat gtcaaactca tctgctagac
     6181 gattagctac tgtttctggt gacttagtcg tgttattggt aacaaacaag aaaggtaatt
     6241 ttcttttttg caattcttcc acaaatcgtt taccggccgg tatgggttgt gtcccacgat
     6301 aaatcgttcc gtctaaatca attaaatatc ctttatagtc cattcatcca ctactcttcc
     6361 ctttttctga tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg ttcttgtttt
     6421 tttacttttt cagaggtttt tgctgactct gtttttcgat ttttaataac cggttttttg
     6481 cgattagatg cgactggtcc ttttttctca tcgatatacg cacgattatg gttttttttc
     6541 ttttttcggc gctgagttgt cttttctttt ttaccgccga ttctctcgat gacaaaatac
     6601 gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct
     6661 ggatgcgccc gtcgattatc cgcttggaaa aatcctttta ggcgtagttg ttcataaccc
     6721 caatctccta cgatataatc atatcgggct agtacatcac tgtaacgttc tccaagcttt
     6781 tctgcattaa atccttcacg ataatcggac acaagccgat aatgtctttc acctatcaaa
     6841 aaattactgt catctaacag tgtcactagc tcgcctttga ctttttctgc tttaggctcc
     6901 tcaacgattt cttccaaagc agctgtcagt tcttcggtga ctgttgtttc ctgttcagct
     6961 gatgattttt tcttagcaat ttctgtttta tgctcaacgt tctgctgttc attttgtttt
     7021 gcagacatgc gttcaccacc tatctttttt attatacctt gtttttattt gattgggtag
     7081 tgagctttca agtaatctcc taccacactt cggataaatt cagggaacaa aaattcattt
     7141 tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta
     7201 aaagtataat ttttattcag atcgattggc ttgttcttcc aaaagacttg atggttaata
     7261 gaatcatatg tgattccatt atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt
     7321 cccttcatag gatagttacg taaaaagtta cgattttttt ctatttctaa aactaagcgg
     7381 atcagatcat ttccacccaa tgtgacattt aacaagtgca tcggatgcgg caacgcagtg
     7441 tgaagctgat cctgattgac ccgccctttt ggaatttcag ttaaaaacag accactgttt
     7501 aatattgctg cttccgtctt tcctcgttct ttcaccgcct caaaggctac ttggataaac
     7561 gaatgctcat tgaagatatc tagactcaga gaaaatggca aatctgctac ttctttttct
     7621 tttaataatt catgcccacg ttctagataa ctatttattt cttgttcatc ttctgtaaaa
     7681 gctgtcattg tttcggtagg gatggctttg gctgaatgct taaggatttt tttctcttca
     7741 tcgatggtta aatggacttc accgacatac tgcccgtact ttccagcggc tgctatttgg
     7801 acatcattga ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc taaaataaca
     7861 tcaattgctg gtatttcttt tgcaatttgg aaatcatcct gaatcccgag atgactcatc
     7921 aaaactaaaa catccacttg tgaacgtaaa tcttcgacta attctggcaa aatatcataa
     7981 ggattccgta tgtcccagcc attcggatta taagtaagcg gaaatggagc agtaaatgca
     8041 attagtccga ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc
     8101 ggtggctgga gcgtattttt atcgaataag ttatccagga tgatatcaaa ctcagcatga
     8161 tcgtataaat gattcaactc ttctttggaa tttcctacgc cttcattgtt tcctatcgtc
     8221 gctgcatcat aaccaacttc attcatcagc aagatatttt cctgtccatt cgttgcttct
     8281 gacaatggat gccaacgatc cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt
     8341 tctttctttt cgttttctcg tttacgttga tccaaaaacc ttctgatcct tggccaattt
     8401 tctaaatgag aatgcagatc attcgtatgt aaaatcacta ttttttccat gtaccctctc
     8461 gcctctcttt atctttatca taccataggc agctttttct tccattgaaa caagctctat
     8521 ttcagagttt ttcctgcctt gaagcaaatt gtataagtac aatattcgat aaatcagtct
     8581 ggctaaaaaa tcaggaacat tcatttttac agtactataa aaataaaaaa aacagacccg
     8641 aaagtctgtt gtcgtttcat tataatcaga cacatggcgg aacctgctta gtgctgcttc
     8701 cttccagacc tgacacgctt cacaagccca ccattgtcct agcctttcgg caaagaatag
     8761 tataccttat ttttcattcg ttgaccagtc tttttttaat ttcttttttc gctggcggag
     8821 ctttcggaaa aagtcagtca aaatcatccc acattcgtct tctaaaatgc caccttctac
     8881 atatgcttgg tggttaaacc gctcgtcctc caaaagattc atcaaggtac cggctgttcc
     8941 gccttttgga tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc
     9001 gctgcacata ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca agcgccagct
     9061 tcccactttt tcacaggctt ccttgattgc aagcatttct gcatgtgtag tagcatcttg
     9121 cgtggtttct ctaaggttat atcctcttcc aatcactttt ccatccaata caaccactgc
     9181 tccaatcgga acttcatgaa gcatttctgc tttctttgct tctgccaatg caaggcgcat
     9241 ccatttttct ttttctactg catcaagatt tgtatttacc aattaccctt ccctgccttt
     9301 gttttttctt gttttacttt agcatatgat aaaaaggctg taactaactt ttttttctat
     9361 ggtaaaataa atacaagagg tgaacaagat gaaaatggat aaattacttg ctctttaccc
     9421 acaagcacag aaaaaaaact ctccagcatc agaagcggat atcctttctt ttgcaattga
     9481 tgatgctttt atctggatca aacaagattc attatctcaa caagaaacca gccttttgaa
     9541 agcgttattc cctgtaatca acgatcaaaa aaaacaccca tggtatcact atctttttaa
     9601 agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa
     9661 aggagaattc ttaaaaaaag aatggcaaga tactattttg gaattatttc gctattctgc
     9721 tgatttcttt ttttatacag acacagatgc gctcttagtc gaacaaataa accgtgatta
     9781 tcttgatgct actgaaatca acggtatttt tctatcttta gatgctgatt ttgatacgac
     9841 aacgtctgta ttcgttggtt ctttccattc acctggcacc gacctcgtcc ctttgtttgc
     9901 tgaagaaaga aaaatttttt taacagagca aaacaatctt tatactcaca gccgtacttt
     9961 ttctatgcaa gatgtggctt tacattacta tacaaaagat actgtaaaag atagtaagtt
    10021 gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt
    10081 atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac
    10141 tctaaaatat cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa aaaaagccaa
    10201 cgatttactg ttttgctatc ttttattgct tcatgaacat aattaaagat agattaaagg
    10261 ggttgtgaca ataattttgt cacaacccct tatttcgaat gaatagtgtt caaaagctag
    10321 tttctcttac atcatttttt caacaaaatg cattagccgg tcttttacat cttcggttga
    10381 atctttgatt gaatcaccat aaacagagat tttctttttt cctgatttga tttttgctaa
    10441 cattcccatc aaagaatcca agtcactatc tgaaagacca tctacgattt ctagtaattt
    10501 ttcatttcca tcaaatttgt catagacgaa tttttttact ttcgtccgag tagtcatatg
    10561 atggatcttt tccattactt ttcccgatac aacgactgct gtcgctacac tggcagcggc
    10621 tgctacgctt aatccgattg ttacttttgt tgatgttttc ataagtatca gctccttttt
    10681 atctatctcc atcatagcat agacaaaaaa ataaacgaac taatccactc agtgaaatct
    10741 caatccttta gggaaaaagt tctttactgt tccttttact tgtttcccaa atccgacagg
    10801 tatcttatct aatactagca aaacccatcc ttgattccca tcacgttgaa atgtttctcc
    10861 tgctgtgtaa gattgccatt ccttttgagt gatcggcaag caaggtatat tctctatctt
    10921 ttttgtagca agcgctaatg catagctagg ttcaaatcga ttctttttga aatctcctaa
    10981 atgcaaacct gtacgtacga cttttaaacc atcgagagaa ggggctaact caggaacttc
    11041 ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct tcataatgaa aatcatttga
    11101 aaactccgtc cagagttttt cctgctcttt tgtcatttgc actttacttt tttttcgtgt
    11161 cttcttttct ttatgcatct gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc
    11221 ttcaccctga tctttatgag gccatatacg tattgttttt tccaaaccag ctacgcttcc
    11281 ccattcagaa cgtcctgaac taacggattg agtcaaagga atctcctcaa tcgttactgg
    11341 ataattttct acaagccagc taatgatctc ttcattttct tctggtgcaa acgtacaagt
    11401 cgaataaatc agttgtcctt tattttttag catttttatt gctgaagaca aaatctcttg
    11461 ctgacgtttt tgacagtata gcggagattc ttctgtccac tcttttattg catttggatc
    11521 ttttcggaac attccttctc ctgaacaagg agcatcaacc acgatccgat caaaaaagcc
    11581 cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc
    11641 ccatcgttct atattttctg ataaaatctt cgcacgtttc ggaaagatct cattcgtcac
    11701 taataaccct ttccccttca tttgagcagc taattgggtt gatttaccac cgggagccgc
    11761 acacaaatcc aaaacttttt caccgggttt tgctgcagca gctgttccaa cgatcattgc
    11821 acttggttct tggctatatt catacccagc ttgatggagg aaagactttc cattgacagt
    11881 gccaagaaat ccctcattgc tgtagggagc gggttgtaat tcttcgctat ggtaagtttg
    11941 gaccatgtcc aatcctgccg gttttagtgg attccaccga aatccttttt tcacgcttcc
    12001 ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac gatatttttt
    12061 gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt
    12121 ttacaatctt ttttacgaaa aataaaggtg gctgatctac tttttcatgt tgctgcctaa
    12181 ggtgactgtt cctttaagat attttccaag tattcatccg tcacctcgtt tttatcccaa
    12241 acagcgttca tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa acttcccaat
    12301 gcattaagcc ataccaattc aagcgacttt tcttctttga tcttcgtgtg acctttgaca
    12361 tatgagaaaa atggccgctt aaatagcgaa tccatgtcta aatcagattt tgcaatgatt
    12421 tcacgaatac tttcatggat agaaaaaaaa tctttcaata agtagacaat gcttaaaaca
    12481 tcatcggaac tgcattcagc taagtagttt tcaaaataaa cagtgatttc ttctaatatc
    12541 ttctctttta acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg
    12601 cagaactctg ctaactctct tgcagtaatt gacttcattc catattcttc cacgtatacc
    12661 atacttttga acagcaaata ttctttacta taatttgtta ctctccccat cattcttacc
    12721 tccgtctctt ttcatctacc cataaataaa taacataaat aattctaaaa atattattga
    12781 taattatcaa aacatattta gttatatcca ttaaaaatca atttttccgc cttaaaaaca
    12841 agaatatctt gacaaaatac cctaggaaat aaacaataga ataaacaagg taacaaaaaa
    12901 attaaataac gttatataaa aaagaaggcg acactctgtg agaataacac agactgaaat
    12961 gactttccac tcacagtttt tgtctaacta tatacaaaaa cagaataaaa aggaggtatt
    13021 tatgctaatt gaagcaagaa aaaaagagtt acttgttcta caaatggtca ttcaaaaaaa
    13081 taattgttct ttacaagaat tatccaatga cttacatatt ccaaaacgga caatcaaaga
    13141 actgatccga aaaataaatg tatcactcaa gcaattttta ttgattgat
//
LOCUS       DAXWLF010000069        12923 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXWLF010000069 DAXWLF010000000
VERSION     DAXWLF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.069"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            133..3660
                     /locus_tag="ACGE4U_001498"
     CDS             133..3660
                     /locus_tag="ACGE4U_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFF8393669.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACGE4U_001499"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACGE4U_001499"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFF8393670.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACGE4U_001500"
     CDS             7448..7789
                     /locus_tag="ACGE4U_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFF8393671.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACGE4U_001501"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACGE4U_001501"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFF8393672.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACGE4U_001502"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACGE4U_001502"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFF8393673.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACGE4U_001503"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACGE4U_001503"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFF8393674.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4389 a         2246 c         2738 g         3550 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggcaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gtgaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt act
//
LOCUS       DAXWLF010000070        12908 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXWLF010000070 DAXWLF010000000
VERSION     DAXWLF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.070"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            55..957
                     /locus_tag="ACGE4U_001504"
     CDS             55..957
                     /locus_tag="ACGE4U_001504"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFF8393675.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            1170..2945
                     /locus_tag="ACGE4U_001505"
     CDS             1170..2945
                     /locus_tag="ACGE4U_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFF8393676.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3011..4597)
                     /locus_tag="ACGE4U_001506"
     CDS             complement(3011..4597)
                     /locus_tag="ACGE4U_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFF8393677.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            4848..5072
                     /locus_tag="ACGE4U_001507"
     CDS             4848..5072
                     /locus_tag="ACGE4U_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFF8393678.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            5213..6964
                     /locus_tag="ACGE4U_001508"
     CDS             5213..6964
                     /locus_tag="ACGE4U_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFF8393679.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            6964..8748
                     /locus_tag="ACGE4U_001509"
     CDS             6964..8748
                     /locus_tag="ACGE4U_001509"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393680.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            9074..9925
                     /gene="licT"
                     /locus_tag="ACGE4U_001510"
     CDS             9074..9925
                     /gene="licT"
                     /locus_tag="ACGE4U_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFF8393681.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            10021..11901
                     /locus_tag="ACGE4U_001511"
     CDS             10021..11901
                     /locus_tag="ACGE4U_001511"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8393682.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(11941..12843)
                     /locus_tag="ACGE4U_001512"
     CDS             complement(11941..12843)
                     /locus_tag="ACGE4U_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8393683.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT         4086 a         2304 c         2735 g         3783 t
ORIGIN      
        1 taccaatata taggaattgt tagtaacaat tgcctttcaa ggaggaatcc atccatgtta
       61 ttaggctcac acgtcagcat gagcggaaag aaaatgctgt taggagcagc agcagaagca
      121 gccagttatg gatcttcgac ttttatgatc tatacaggcg cccctcaaaa cactagaaga
      181 aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt acatggcaga aaacggtcta
      241 acaaatatcg tcgttcatgc cccttatatc attaatttgg ggaatacgat caaaccagaa
      301 atgtttcctt ttgccgtcca atttctgaga gaagagattg ttcgtgcgga agctttaggc
      361 gctactcaaa ttacacttca cccaggtgct catgtagggg caggaacaga agccggaatt
      421 gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag aacagacggc acaaatcgct
      481 ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa catttgagga attagcgcaa
      541 atcatcgatg gagtaaaatt aaatgaaaaa ttatccgtta cttttgacac ctgtcatacg
      601 aatgatgccg gttataatat taaagaagac ttttcaggtg taatggaaga gtttgataag
      661 atcatcgggt tagatcggct taaagtactc catatcaatg attctaaaaa tccgcaaggt
      721 tcgcataaag accggcacgc caatattggt tttggaacaa tcggattcga cacgctgaat
      781 aagatcgtcc atgatccaat gtttgacaat atttcgaaaa tcttggaaac tccctatgtc
      841 ggcaaagata aaaagaaagc ttacgcacca tatggaaaag aaatcgccat gttcaaagca
      901 caaatattcg atccagaagt gtttcctgag ttattagcag aagaagtgat actctaagat
      961 tttctatctg acagcagcaa aaaaggacca ggcacattgc cttgtccttt tttgtttaag
     1021 aaacaaagga gtactttttg cagtcttttt catcatgcat cttctccccc ctctttttaa
     1081 atttagacaa acaaggtata ctgttagaaa agtaaaaaaa cgaaaagttt cacaagcaag
     1141 tggcaaaacg tgattggaag gacgaaaaga tgctgaacac tttaaaaccc ttgatcaacc
     1201 aactaaaaaa gaaacataca tactttattc ttggcatagc cctattattt acaattattc
     1261 gtttgtattt atatagtaaa gcaatctatg gaacagacat gtcggcaacc tatgatgacc
     1321 agctgctgat tcgctatgct caaaatattt tggacggtaa atggctaggg gagtatacga
     1381 ctacaacact ttccaaagga atctcctaca gcttattttt agtacttgca cataaactgc
     1441 atctttctta tgctgttttg ttcggcatgt tcagtatttt tgccagtata ttggttgcac
     1501 tcgcgtttaa accgatcgtg aagaacaagt atcttctttt aagtatctat ttttattttt
     1561 tgttttctcc gattagtttt acacatgaat attccactcg aacatatcgt aatgccctcg
     1621 tgattccggc tgttgagatc attatcgctt gtttactagc tatttattac cgcaaatacg
     1681 gttctagcag aaaattgctg ccatggtttt tagggctttc tgtcgctttt cctttctttt
     1741 ggttcattcg cgaagattct atctggcttc ttccttttac tgtagtagct ttgggtatca
     1801 gtttcgtcca gatcttgcta gggcaagact tttctttcaa agaagtaaag aagttaaaaa
     1861 aacgactctt tcttctctcg aaacgggaat ggctgaaact ttcattgttt ttacttccta
     1921 tagttggctt ttccatcgtc tatcagtgga tcgaatataa aaatgagaca acatacggta
     1981 ttgcaaccat caatgaccgt tccagtggag aatttggacg tttgatgaaa caactgatcc
     2041 gaatagatga tgaaacgaac ttgaataaag aaaacagtga gatatgggtc tcacatgctg
     2101 cactaaataa agcattagcc gcatctccta cattccatac tgtttctacc cgtattgacg
     2161 aattatatac gactcacgcg tggactcaag gtggaaaagt aaaagaatta cccggagata
     2221 tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa
     2281 aagaaaccaa tgatttttgg aaaaaagtca accaagaatt agaagaagcc tatcacaagg
     2341 gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc aggagatgga aagacgatga
     2401 gtgatctgcc aatcgtattc gatttccttc aatcaggata cgcttatgca tggcgatata
     2461 aagatttcca tcaaggagga aattatagtt tcggaacgca agaacaagtc aaacaggctc
     2521 aagaattatt aaaccttccg ctaatggaga attggatcga tagtaatgaa ccgagaacac
     2581 ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa atttaccaag
     2641 ttctggctat tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa
     2701 ccgtatttgc aaaagctgat cgtgcctatt atagttcctc tttgattgtg gtcaccggat
     2761 taatactgac acaattggtt tttcttttcg gtgtctcgtg gttttgttct tatgcgccag
     2821 aaaaaaagga ttatttttta agtgtctaca ctggcgcagg cgtgccatta acgcaattca
     2881 tcgtagtaac aagcatgcta gttttcagta aattaaatct gaaaaaggct ttatcttcta
     2941 aataaaaaga cccttggtag ctagattttt aacctaacta tcaagggtct ttgtgtggaa
     3001 aaatcagttt ctattcgatt tcttctaccg tttcctgcaa acgctgttca tattttttgt
     3061 ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga acaatgatta
     3121 cataagcgat gtgatcagcc aatgctagtt gagcagaagt ggcttgttca ccaaagaaac
     3181 tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt tagactaatc acagagaacc
     3241 agccaagaag ttttacgatg aatgaatttt taaatcggct acccatctcc gcttggctgt
     3301 tggtcatcat cagtaaaggg atcatcgaaa acggcaacgc aaaagccaag aacacctggg
     3361 attcattcat caagttattc aaagcggtat gttcttcgat cgttcctttt gtacttgtaa
     3421 acaaaacaca aatcaataca ggaatcacag aaagcaatcg tgtgaccagt cttcttagcc
     3481 aaatcgggat acgcatatgg atgaagcctt ccatgatgac ttgaccggtc aacgtaccag
     3541 taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg gataagatac
     3601 cagattttgc tacagcaatc aagataccat tgctcatagc tgatggatca gataaggctt
     3661 catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca gcgacaccca
     3721 tgatcaatag cagtgcattg actagaaaag ccatcgacaa ttggatatta gagtcccaag
     3781 tagaaaaacg tacagcttgt gctacatctt cttcgtcttg gtgatcaatc ttccgagttt
     3841 gagaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca ataatcccta
     3901 aagctccagt cagcggagtt tcgccgccga tgaccggtga gtctgagaat gtatgactgt
     3961 tagggataaa gccagcaaat aaactgtgcc aatctgggtt agacaagcca acttgataga
     4021 caaaaacgaa taaaatgacg aaaattaagc agacaaccag tgcttcgatc ttacgaaacc
     4081 cgacttttgt cagtagtaag agaacgaaaa catctaatac agtaataaag acggcaatca
     4141 ccaatggaat atcaaaaagt aagtagaggg cgattgctgc accaatgact tctgcgatgt
     4201 ctgttgccat aatggctaat tcagtcaaga tccataaaat gacacccaat gtcttacttg
     4261 tgcgtgctcg gattgcttgg gccaaatcca tttgggtcac gattcctaat tttgctgcca
     4321 tatactgtaa aagcatagca atcagactag acatcaaaat aactgacatc aataaatatt
     4381 ggaaattttg tccaccagtg atcgaagtgg accagttacc tggatccata taaccaactg
     4441 ctactaatgc acctggtcca gagtaagcaa gaagtgtttt aaaaaaactt ttcccctttg
     4501 gtacatcaac tgttccattt acttcagcta aagataaacc atttgtatgt tcaataagtc
     4561 gatgcttatg aaattgtttg ttggattgat tattcaagaa aaaaccacct ttcctattta
     4621 tctacgatta cattctacac ctcaaaaaga aaaaattaaa gaaaaatatt cggtatgcct
     4681 aatttatttt cgtggcgcgt cttgttagat gacgaaagaa attttgttct cctctaaaaa
     4741 aaccactatt ttttgcatga aactaaagaa tacgcctttc ttttttcaat aaactttagt
     4801 aaaataagtc attgagagca attcggaaat agaaaggaga gatttatatg ttatcaacag
     4861 gtattgtcgt acttattgct attattgctt tgctaatcgg cgcagtaggt ggctttttcc
     4921 tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc tcccgttaat gaggacatgt
     4981 tgcgatcaat gatgatgtca atgggacaaa aaccatctga gaagaaaatt cgccagatga
     5041 tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac ccaacgactt tttacgggct
     5101 gattttttca gtccgttttt tattttgtaa tttgttagac tattcagatt attcagcaaa
     5161 aagacaagca cagacaatcc taggctcttg ataaagacaa aggaggagta aaatgtcgat
     5221 ttttagaaag ttaggctggt tttttcgtca ggaaaagaaa cattatttaa ttggggtttt
     5281 ctcgctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
     5341 tgatgagatt gccgataaaa atttatcagt aaagatcatt ttcgggtgga tcttcctatt
     5401 gattatcgct gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     5461 aagtgcagct cggttggaga aagaacttcg gcagcagtta ttcaaacatt ttacaaaaat
     5521 ggacaatctc ttttaccaaa aatatcggac tggggatttg atggctcatg caacgaatga
     5581 tttgaatgcg atccagaatg tagcaggagc tggtatcttg acctttgcag attcagtgat
     5641 tacgggcgga acaacgatca ttgtgatgat tttgtttatt gattggcgct taacactgat
     5701 tgcgctgatt cctctgcctt tgctggccgt cacttcacga gtgctggggt cgaaactcca
     5761 tgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     5821 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tagcggattt
     5881 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttc tagactcctt
     5941 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     6001 tggaaagtat gtcttggaag gcacgatctc gatcgggcag atggtctcat ttattagtta
     6061 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     6121 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaaaacgc atatcgtgga
     6181 acaactgggt gcagtgcaga ccccggtaaa aggcgagctg aatgtagcta tttcctcttt
     6241 taattatccg aaagaagaat caccagcatt agaacaaatc aagtttgcaa tccaagaagg
     6301 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acaacgatca tcaaattact
     6361 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga
     6421 atattcattg aatgctttat taggatcaat cggctatgtg cctcaggatc acttcttgtt
     6481 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     6541 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattac caaaaggtta
     6601 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac aaaggatttc
     6661 gattgctcga gcattgatca aaaatcctga attattaata ttagatgatg cactttctgc
     6721 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     6781 aacaactatc attgcagctc accgtttaag tagcgtgatg catgcaaaag aaatcattgt
     6841 tgtcgatcaa gggaaaatca ttgaacgagg aacccatcat gagctgcttg cactaggcgg
     6901 ctggtatgaa caaatgtggg agaaacagca attagaagcg aagatagaag gaggcgaggc
     6961 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaaa
     7021 aagatcatca aacgactgat gaagttcgcc aaacctttta gaacaacgtt catcgttgct
     7081 atcttatttg cgtttgctct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     7141 tttatggatg atcatctggc taaacagtcg gctacgacac aagttatttt gttttttgct
     7201 ggtatgtact tatttggagt cctagtaaaa agtatcattt ggtttttcca atggtatttg
     7261 tattccatgg cttcattgaa gacctatcag tatattaggg taaaattatt tgaaaagctg
     7321 catacactag gtatgcgtta ttttgatcag acaccggcag gatcaaccgt ttctcgagtg
     7381 acgaatgaca cagaaacgct atttgaattt tggtacgtct ttttgatggt attgactggg
     7441 atctttgcag tcgtttcttc attcattgcc atgtttcaaa tcaatggaga aattgccttg
     7501 tggactttgg tgttcttgcc gattttagga attgtcatct ggtattacca gaaatttagt
     7561 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaatacaaa attgaatgaa
     7621 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
     7681 tttgaacaag tgaacaatga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
     7741 attctgttaa gtccgatcat caattttcta tatgcattgg cgattgcgtt ggcgttgact
     7801 ttatttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
     7861 acttatgtcc agggattttt taatccaatg tctcaaatga tggacttttt gagcatcttc
     7921 acagatggga tggttgctgg tagtcgtatc ttgaagatta tggatacgga agaatatgca
     7981 ccacaacaaa accctggagc agatcaaatg attacttcag gtaagattga gtttcgcaat
     8041 gtaagctttt cttatgatgg agagcacaat gtattaaaca atatctcttt tgttgctaat
     8101 ccgggagaaa ccgttgcatt agtgggacac accggaagtg ggaaaagctc gatcatcaat
     8161 gtgttgatgc ggttttacga attttatgaa ggtcagatat tgattgatga tcatgacatt
     8221 cgagaatatc caatggaaga gttacgtaag aaaatggggc tggtcttgca agatgctttc
     8281 ttgttttatg gggatatcgc aggaaatatt cggatgatga atccagctat cacagacgaa
     8341 cagatacgcc atgcggctga gtttgttcaa gcaaacaaat tcatcgatga attaccaaaa
     8401 ggatatcact caaaagtgat cgaacatggt gccagctatt ctagtggaca acgacagctg
     8461 atcacttttg cgcgaacaat cgtgactgat cctaaaattc tagtattgga tgaagcaaca
     8521 gcaaatatcg atactgaaac agaaggattg atccaagaag gattggcgaa aatgcgacaa
     8581 ggacggacaa cgattgcgat tgcccatcgg ctgtcaacga ttcgagatgc agaattgatt
     8641 ttagttttag acaaaggtca tatagtagaa cgcgggaatc atgaaacact tctagcgcaa
     8701 aatggtttgt atgcagatat gtacaaactg caaagtagtg gtatataaaa gatgttgtga
     8761 cagtagcatt ttgacctaag cactagagtg aaaataaaac atagcttccc atatttttca
     8821 tctgtgagag ttagtgtcgt agttaccaga tttaaaaaag acgacgtcag ctagtcagaa
     8881 gtgcctaata gcccctaaga aagcgttgac aaaatcgttt gctttcgata atataaagaa
     8941 aacgatacaa cttggatagt gattgcctga tgcaagctaa acctgatgag tctcccatgg
     9001 tttcatggac ggctttttgg gtttttcttt ttgttttgga tacagcccta tatatcatgg
     9061 aaaggagcaa aacatgctga ttgagaaagt actgaataac aacgtcgtaa taacaaggga
     9121 tgaaagagat cgggaaatga ttgtgatggg aaaaggattg gcattcaaaa agaaggtggg
     9181 tgacccgatt cttgactcat gcatcgacaa aaaatttcag ttgacaaatg acggactttc
     9241 acaacagttc caagaattgg cacaagcgat tcctcttgag tacttagaaa tttcttgtga
     9301 aatcattgaa tatacaaaag aggctctgca agtccattta aatgattctg tgtatatttc
     9361 attgacggat cacttgtata cagctatcga acgtcaaaaa atggggatct ttgtgccaaa
     9421 tgtattgctg tatgatatca aacaactgtt tcctaaagag tatgccattg ggaaaaaaac
     9481 agtgaagaaa atctcggata aataccacat caagttatca gaagacgaag ccggatttat
     9541 cgcattgcat ttagtcaatg cacaaggcat tcaaagccag caagacaata cctacctgtt
     9601 taccacaaca atacaggata ttttaaatat cgtccgctat tactataaga ttacatttga
     9661 tgaagattca gtccactttt ttcgattcgt cacacacctg cgtttttttc tgacacgtgt
     9721 cctagatgga gcgaaatcag aagatggaga agtttctgag gaattgttcc aagttattca
     9781 aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt taaccggggc
     9841 tttcgactac cagatgtcca cggatgaaaa aatgtattta acgatccata ctgcgcgttt
     9901 agtgcatgag aatcgttgta aataaataaa aaaggaggac aaattaacaa gaacgtatga
     9961 aataagggta gtgacattca gttggctaaa ccttccacta attaattgga gggaatagaa
    10021 atgggaaaat accaggaatt agcagaaaac atcgtaaaga atatcggagg aaaagaaaat
    10081 atccgtagct tgacacactg tgtgacgcga cttcgtttca aactaaagga ggaaaataaa
    10141 gcaaaggatg atgttttgaa aaagatggaa gggatagtca ccatcatgaa aagcggcggg
    10201 caatatcaag ttgtaatcgg gaatcatgtg ccagatgtct atgcagaagt actgacagta
    10261 gccgggattt ccgatgaagc agaagaagag gtttcttcaa gtagtaatct gttcaatcga
    10321 ttaatcgaca tattaagtgg ctgtttccag ccttttttag gtgctttggc tgcagcggga
    10381 atggtcaaag gattaaatgc tttgttcttg tttctcaaat tttatactga aggatcgggt
    10441 acgtacatca tgctgaatgg gataggggat gcaatttttt atttcatgcc agtgatctta
    10501 gggtacacag cagcgaaaaa atttcatctg aatccgatgg tggggctcgt gattggtgca
    10561 gcactttgtt atccgactgt acaaggagca gcactacaaa gtggtttccc tgatggcagt
    10621 cctgcgcctt atagcttgct gggtcttccg gcttatgaaa catttttggg tatcccatgg
    10681 gtgggcagca gttatactag tagcgtcgta cctgttatct tcattatcgc atttgccgga
    10741 caagtccaaa aaattgccaa gaaatggatt cctgcagtcg tccagacctt tttggtttct
    10801 ttttctgtgt tattaatcgc acttcctgct ggctttttga ttatcggtcc gattgtcagt
    10861 atactgacag atctattaag cacgggattc caagcattga tggacttttc tccagctgtg
    10921 tatggtgcag ttctagggtt tctttggcaa gtactggtga ttttcggact tcattggagt
    10981 attatccctc ttgcgattat gcagctagcg caaaatgggt ggagtcagat gttagtaggc
    11041 gtttatggcg cgagttttgc acagacagca gtggtattgg cgatgtattt caagttcaag
    11101 gatcaaaaaa tgaaggcgct ttgcatgcca gctgtgattt caggaatctt cggcgtaaca
    11161 gaaccggcta tttatggaat caccttaccg aagaagaagc catttattta ctcgatgatt
    11221 ggtggtgctg tttcaggaat ctatttgatg gcatctggaa caatcagcta cataatgggc
    11281 ggattaggga tttttggggt ggtaaactac atcacggaaa cagacgccag tggtatgttt
    11341 caagcgttta tcggcattgc tattgcttcg gtaatcgggt tttcgctgac ttttttcttt
    11401 tggaaggatg aagcaggtga aacagatgct atacagaaaa aatcaggtaa agaaattgta
    11461 aaaacaccga tacaaggaca aatcgctcca ctaaatgcag cgaaggatgc agcttttgca
    11521 caaggaacac taggtcgagg aatcttaatc taccctgaaa aaggagaagt acgtgcccct
    11581 tttgacggaa cagttatgac cctgttcccg acgaaacatg caatcggaat ggtttctgaa
    11641 acagggctgg aattgctgat tcatgtcgga ttggatactg tgcagttgga agggaaatat
    11701 tttgaaagct tagtgcagca aggagaaaga gtgagacaag gcgatctgtt gctacgcttc
    11761 gacaaaaaga aaatcgaaaa ggaaggatac agtttagaaa cacctgtgat cgtcaccaat
    11821 tacacggatt atttggatat tttggaaaac caaggggaga cggtcgggcc agacgatgca
    11881 cttatcaccg cactgacctg aacggttagg tgaagaatgg actaggctta actattcttt
    11941 ttattccact tctttatttt caaaattgaa aggctctttc atgatggctg agctgatagg
    12001 gcgtttatcg aatttttgac cgacagaaat tagatggact aaattctgtt gataattttc
    12061 tgcaaaaaca caactatata acacgtctag aagataacaa attgatgtat tgatcgtaaa
    12121 attaccaatt tttgaataca gtcgctcgcg tgtcgtaagt cgcaaagcgg ctgtactcat
    12181 tgcagatagc gttgaatcac cgatactggt caaagctaaa attggaacat cattttgttt
    12241 caatatttct gcggtacgaa ggatcacatt gttttcaccc gtataagaaa tcagaatccc
    12301 gcacatatcg ctcgtacaat tgtacgcttc atagaatgat tcaccaaaag tggtgacaat
    12361 tgtgacatcc attttgatgc gcttcatttt cagcatgaaa tcttgagaaa tcaaagtatt
    12421 cgagttactg gcgaagatca cgattttttt tgcctggagc agtaaatatt tagctttttg
    12481 cagttggtca tgatgaatga gagaaagtgt atcatgaatc gtcatctgtt ctaaagaagc
    12541 cattttattt gcgattgtca taataccgtc tgtggtggaa aaaggaagat tcgcatctac
    12601 gtcttgaaaa ttcccttcaa gataggctgt ttcagctaaa aaagcattac gaaaactgac
    12661 ccagccatca aaacccattt tttttgctac acggatgagt gtagacgggt ggacataaca
    12721 tcgctcagct atcgcttgga tggtcatttc gccaagtgct ggagaatcgc ttaagagaaa
    12781 ctggatcact tcattttctg aaggagaaaa ggagatttct ttgatttttt cacgtaataa
    12841 catgtagata ccttcttctt atttttttac gtaaattata acttttttga aaatgttttg
    12901 taaacgga
//
LOCUS       DAXWLF010000071        12811 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXWLF010000071 DAXWLF010000000
VERSION     DAXWLF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.071"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(113..310)
                     /locus_tag="ACGE4U_001513"
     CDS             complement(113..310)
                     /locus_tag="ACGE4U_001513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393684.1"
                     /translation="MIVKETKWMIDSPDYCTCNNNKNIHTEFNDQGSLSICDNCHKLV
                     KDSFKTFESYPVQNYVKSRRR"
     gene            complement(372..797)
                     /locus_tag="ACGE4U_001514"
     CDS             complement(372..797)
                     /locus_tag="ACGE4U_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393685.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1171..2142
                     /locus_tag="ACGE4U_001515"
     CDS             1171..2142
                     /locus_tag="ACGE4U_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8393686.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2174..3571
                     /locus_tag="ACGE4U_001516"
     CDS             2174..3571
                     /locus_tag="ACGE4U_001516"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8393687.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3730..4353
                     /locus_tag="ACGE4U_001517"
     CDS             3730..4353
                     /locus_tag="ACGE4U_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393688.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            5102..7195
                     /locus_tag="ACGE4U_001518"
     CDS             5102..7195
                     /locus_tag="ACGE4U_001518"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8393689.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            7355..8221
                     /locus_tag="ACGE4U_001519"
     CDS             7355..8221
                     /locus_tag="ACGE4U_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8393690.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(8243..8680)
                     /gene="ndk"
                     /locus_tag="ACGE4U_001520"
     CDS             complement(8243..8680)
                     /gene="ndk"
                     /locus_tag="ACGE4U_001520"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFF8393691.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     regulatory      8853..9027
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            9369..12017
                     /gene="mgtA"
                     /locus_tag="ACGE4U_001521"
     CDS             9369..12017
                     /gene="mgtA"
                     /locus_tag="ACGE4U_001521"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFF8393692.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     gene            12104..12775
                     /locus_tag="ACGE4U_001522"
     CDS             12104..12775
                     /locus_tag="ACGE4U_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8393693.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
BASE COUNT         4290 a         2149 c         2674 g         3698 t
ORIGIN      
        1 tttgtaaact gaaatgatcc actgtttgtt ttactgatga agcttgatca aaggaagaag
       61 ctgagtttga agttcataaa aatcagccat agtgagtttt gcttggattt gtttatcttc
      121 ttctactttt tacataattt tgaacaggat aagattcaaa tgttttaaat gaatctttga
      181 ctagtttgtg acagttgtcg catatgctca agcttccttg gtcattaaat tctgtatgga
      241 tatttttgtt gttattacat gtacaataat caggactatc gatcatccat ttcgtctcct
      301 ttacaatcat cttgcttctt tactatagca gaaaagaagg ggagaaacaa aagaagatcc
      361 aacagacggc actatagact gtagatgttg ccatcctcat ccttcaaacg gtaattaccg
      421 atttttgtaa atatgactgt tgtttctttt ttattttctt ttcctgaaat aatatattcg
      481 tcatctttgg tagtgatctt gatatctgtc agcactgttt tttcttcaga tgattcagaa
      541 gaatcagttc cttgtgtctg atacgtgcgt gtccattcca tttttttatc tgtaaatgtc
      601 aactgtcccg cggataaatc catatcttta cgtactttta gcgtatatac actgttggtg
      661 gtatctgctt tttccgcttt taagacagaa gaatcggttg tttcgtccga ggattggtca
      721 gagccgccaa acaaatcaca gccagtcaat gtcaaaagtc ccgcagacaa aataaatagt
      781 acgattaatt tcttcaaaat acttccccca tttgatagga taaagaaatt ataccattgg
      841 aatgtaaaac ttcaaacgga tggaagagaa aaacgcacaa aacacccaca aatagagtaa
      901 attttagaat aaaattttaa aaaggcaaaa aatccgccac tttcagaaag cgttttaaaa
      961 aagtgttgca atcttttaaa aaaatgctat attaaaaatg aaccggttca aatacttgtt
     1021 taagtataat taatagtgag aggaagttgc tctactgaag actttagtta tatggatatt
     1081 gatgacaagc aacactatgc aaagaatgcg gtcatcataa tcgaacaaca agaaagagaa
     1141 atcaaatgta actggcagga gtgagaatgt atggcatcga tccgtgatgt ggctaagatg
     1201 gctggtgttt cagtgggaac agtttcccgt tacttgaatg gtcagcaact gaaggaaaag
     1261 aacatgaaaa aaatagcaga agcgatttca gcgttggact ataaagaaaa catcattgct
     1321 aaagggttga agaataatcg aagcttctct attggacttt tgataaacgg gatttccagc
     1381 cgttttgggt cggaagttgt ctcagggatc gaaagagtag ctgaagaaaa tgggtatagc
     1441 ttattattaa gcgggtttgc cgatcagcca gaaaaaattg aacaaaaaat tgaatacctg
     1501 atgaagcatg tgatcgatgg gctgattgtc ttcttatccg aagaagagtg gtctggattt
     1561 gaaatgctca caaaaatgag tattccagtt atcgcattga attgtccaga aggaccagca
     1621 ggtattgata caatattagt gaatgatcga gaaagtgtag caaaagtgat cgctcatcat
     1681 ggtgaacaag gacataaaaa aatcggattt attgctgcca ctcaaacaga ctacgtagca
     1741 agagaacgtt tagcaggagc caaagaagcg gtccaaaatg atccatcgat ccaattagag
     1801 gtctttacag gagactattc tagaaaaagc ggctattacg gggcaaaagc attattagaa
     1861 aaaggggcaa ctgctatttt tgtcagcaat tacaatatgt cgattggtgc catcgaatgt
     1921 ttcaatcaag aaggtgtcag aatcgggaag gatattgcgt tcagtcacta tgattattta
     1981 gataaaaacg atctgagtat ccttccgaaa atcacgatca cgccaccaac agataaaatc
     2041 ggggaacgag cagcagaaca gctgttaaaa cgaatcaaaa aagaagagga agaaaatgaa
     2101 aaagtgattg tgatggaaaa tatcatacat ggaatcaact aaaacgaact aaaaaagaaa
     2161 aagaggtgct ttgatgaaaa aatatcaatt tccagcaaat ttttggtggg gttctgcagc
     2221 aagtggccca caaacagaag gaaggatttc aggcgatgga aaaggagaga acatttttga
     2281 ttattggtac aaaaaagaac cgcaaaagtt ctttgatcaa gtaggacctg aaaaaacctc
     2341 gcaagtttat acgaagtatc aagaggatgt acagttgatg aaacagactg ggcataattc
     2401 atttcgaaca tctatccaat ggagccgttt gatacctgat gaatctggaa caatcaacga
     2461 agaagccgtt cgtttctatc atcgttattt tgatgagttg attgaaaacg gtatcgaacc
     2521 ctttgtgaat ctgtatcatt tcgatatgcc gatgtatctg caagaaaaag gcggatggtt
     2581 gaaccgagaa acggttgaag cctatgaaaa atatgccaaa agttgttttg aattgtttgg
     2641 ttcgaaagta aaaaaatggt ttactcataa tgaaccaatc gttccagtcg aagcaggtta
     2701 tttatatcga tggcattatc cagaagagtc agatatgaaa aaagcgatcc aagttggcta
     2761 tcatgaagcg ttggcttcag cgttagccat caaatcttac catgaaatgg agcaaggtgg
     2821 tgttattggt atcatcttga atctgacacc aagctatcca agagacgaat caaaccctga
     2881 agatgttaaa gcagcgcaaa tagcagatgc gttcttcaat cgttcattcc ttgatccagc
     2941 tgtaaaaggc gagtttccaa gtgaattgat tgagatagta aaagaacttg atctcatgcc
     3001 tgtgatccat gcagaggatt tgacggttat caaagaaaat acagtagatc ttttaggtat
     3061 caattattac cagccacgcc gggtaaaagc aaaagaaaca ccaatcgata ctaaaaacgg
     3121 tccaatgcct gaggattatt tcgacaatta tgaaatgcct ggtcgtaaaa tgaatcctta
     3181 tcgcggatgg gagatctatg aacaaggaat ctatgatatt ttgatcaatg tacgagatca
     3241 ttatggaaat atcgactgct ttatttctga aaacggtatg ggtgtagaaa acgaagcacg
     3301 atttatcaaa gaagatggca tgatccacga tgattatcgg atcgaatttg tccaatctca
     3361 tctgaaatat gtccatcaag ccattcaaga aggtgccaat tgtttaggct atcatatgtg
     3421 gacatgtatg gataactggt cgtggaccaa tgcatataag aatcgatatg gctttatttc
     3481 agtagactta gcacaagatg gaaaaagaac aatcaaaaaa tcgggtcatt ggttcaaaga
     3541 cgtatcagat aaaaacggat ttgaggctta agaaagaata gttgagagca aatgcgtgaa
     3601 attctccatt cttaccattt tgtgaaaaag aagttcaatt atttacgatc tttttcaact
     3661 tttttgtctt tctatttcat tttttgtata taatagaata gtcaaaatta aataggagtg
     3721 gaggaaagca tgaggaaaaa aagaccgttt cttctctttt ggttattcgg ttttttctta
     3781 ctgcttattt tatccgcagc attttcagga aaaatgacag cagatcttgc tcagaaagaa
     3841 acagaaaaaa tcgtagcaag caaagaatca gtagataatt tcaaaaaaga attctcaact
     3901 tattggtggg cggagtataa aataactgcc aattacgaaa aaggcgaatt ggacatttat
     3961 ttgccaaaag gaaaagaaga aaatcaattt attaatatcg atgacgtact tagtgcgatt
     4021 tctctcagac aatatcaatt taaagacaag catttgaccg ttttgctttt gactcatgat
     4081 ggcgacaaag tagcccgttt gaaaaatgga aaagtgatca ccgcttattc tgaaaaagta
     4141 gacaaaaaag tggtcgccga tcgtttagcg gaatggaaaa agacaaatga acaagtcaag
     4201 aaaacaactg tctcttctca aaaaaacgag tcgacaggaa caagtccttc agattctggc
     4261 agtggacaaa cgataacggg aagtgaagaa aatcaagcct caattattac gaatgagtca
     4321 tcacaaggac aatatcaatc ctatggacaa tgaaaaacag aggttgtgaa atcagcgttt
     4381 tgacctgagt attagagaaa caaacggcaa aatagttcct catattttgc cgttttttga
     4441 gctaataccg aaggtcagct gattgaacac cgtgtatcaa gaggtcttga gcctgccgtt
     4501 taactctgag ccacaaggag caatttcaaa aaatagctcc ttatattttt cgtttggttg
     4561 ggcttattac cgcagatgca agcttttgaa caccgtttac caagaggttg taaaattagc
     4621 gtcctgacat aatctaatac cgagggtcag ctgattgaac accgtgtatc aagaggtctt
     4681 gagcctgccg tttaactctg agccacaagg aacaatttca aaaaacagct tctcatgttt
     4741 tataaatatt gtgacttgtg gccgaggagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtgaaaaag ctgtcctgca tcaagaaata aggagctgcg acaagaattt tgtcacagcc
     4861 tctttttttg tacgaatacc gagaaactgg cttttgaata ccgtggagtg aagcggtcac
     4921 cacaagactt ttatctcttc attagtttaa tcgcttacat cgcagcggtt ttatgctgtg
     4981 aaaagtggac ggaataatct ttagaaagaa taaaaaaatc ttaagataac aactttttta
     5041 ggaaattttc atttttcagg agcaaaataa gaaaaaatgt gaatagagag aagtgatata
     5101 cgtgacacaa cttaaaaaat tgtgccaaaa tcgtttagca atcgttttga cagctgtttt
     5161 ctttttctgg ctgaagacga tcattgccta ctatgcagat ttttcattag gtgtggaagg
     5221 tacgatccag tactttatct tgtggatcaa cccaattgcg actaccttat tgttttttgg
     5281 cctttctttg tatattaaaa agccaaagcc agcgttagct attcttttga tcattgatat
     5341 tttaaatacg cttttgttgt acttgaatat tattttttat cgggaattca cagatttcat
     5401 tacagtcaaa tctgtattag gcttttcgaa agtgtcgcaa ggattgtcag gaagctcctt
     5461 ttctttgatg aagccccatg atgtcatcta ctggctggat atcgctgtgt tcattgggct
     5521 actggtttgg ttgaaggtca agaaaatacc tatcaaaagc aaccctgtcg gcaaaccgat
     5581 ggctatcgct gttacctgtc tttcagggct tattttttca ggaaatctag cgctgagcga
     5641 agccaaccgt ccacaattgc tgcagcgaac atttgaccgt tcatatattg tcaaatatct
     5701 aggaatcgat gcgtatacta tttatgacgg aatcaaaaca ggaatgacta gcagtgttcg
     5761 cgcgcatgca agcagtaatg ggattgatga agttttagat tatacaaaga aacattatgc
     5821 agagccaaat cccgaaacat ttgggatcgc taaagggaaa aatgtgatcg ttctccattt
     5881 agaaagtttc cagcagtttt tgattaatat gaaggtagac ggacaagaag tcacaccgtt
     5941 tttaaatagt attttccaga atcaagcaac gatcagcttt gacaatttct ttcatgaagt
     6001 aggacaggga aagacaagtg atgctgaaaa tatgttggaa actggcacat ttggtttacc
     6061 tcaaggctca ttattcacag aacttgggtc agacaatgtc tttcaagccg caccagcgat
     6121 ccttggacaa aagcaaggct atacaagcgc tgttttccat gggaatgtag ctagtttttg
     6181 gaatagagat catgtatata aaaacttagg ttatgacaat ttctttgatc gatcttattt
     6241 tgatgaatcc gacgaaacgt taggttatgg gatattagat aaagatttat tcagagaatc
     6301 tgcccaatat cttgagcatc tacaacagcc gttttacacg aaatttcttt ccgtaaccaa
     6361 ccacacgcct tattatacgg atgacaagaa tttcgacttt ccatctttga atactggcaa
     6421 tagcacagta gataattatg ttcgaacggc acattatctg gatcaatcat tggaacaatt
     6481 ttttacgtat ttgaaaaaat ctggtattta ccaaaattca atctttgtga tttacgggga
     6541 tcattttggg atctcgaata cagataataa agatttagca agcgctcttg gtaaagatcc
     6601 agatacatgg gatgaatttg acaacgcaca gatgcaacga gtgccattga tgatccatat
     6661 gcctggttat actaaaggga cagtcaatca tgaatacggt ggagaaatcg atgttttgcc
     6721 gacattacta catctattgg gaatcgatga taaagattat cttcattttg gtacagactt
     6781 attatcttct cagcatgatc aagtagttgc ttttcgaaat gggaactttg tgacacccaa
     6841 atacaccgtc ctaggaggaa aagcttacaa taatcagaca ggagaaatca tcgatacgaa
     6901 agcagcagga atcgatgagg aaatctccaa agatcaggaa aaagtcaaga ctgcgttaag
     6961 tctttcagat aagctgaatc aggaaaatct cttgcggttt tatgtgccaa acggatttga
     7021 gacgattgat ccgaaaaaat atgactataa aaatgaagcg gaacgtaatg aagcaatcga
     7081 aaagcaaaaa ggcgaacatt cgacaagtgt cttttcaaaa aatggtaata aaacaacaac
     7141 caacttatat ccagtaacaa gaacagacgt tgaacagcaa aaaaataatc aataaacaat
     7201 caaaccaacc ttcagtagtt agacaacatt ctaatgcttg aaggttggta ttttttatga
     7261 aaaaaacatt tttttatttt tttcgtgtat ttttcctttt taacgtatct atttattgaa
     7321 tgtggaaaaa tttaaattaa aaaggagaac tattatgtat gatggtgaat tgatcaagaa
     7381 acttcgagtc aacaagaagt tgacacagtc ccaattagcg gaaggaatct gttttaagac
     7441 ttctctagtc gggattgaga caaattcagt gaaaaaaatg tcatttgtaa cattgagatc
     7501 atttttagag cgaatgaaca tgacacttgc agaatatgaa tggatgcgaa accagctgga
     7561 tgaaccaata aagttaaaga aaaaaagacg aatgctagat cgggtacaag aagacagctt
     7621 tgatccttat aaagagatca gcaataaccg taggcatttt aagaaaacag aagatctgta
     7681 ttatttggtt ttgaatcttc acatggtcga aaaaacagaa gtatctgaga cagaatttga
     7741 atttttacgt tatgaatgcc gaatcattga agaatacttg atgcagatac cagactatgg
     7801 tcaatttgaa ctatctattc tagctgattt tccttggata ttcagtgatc ggttcattga
     7861 gaacaattat ttgaaaatca aaaaaagaat gcgacgatta gcacagttga acaatcatga
     7921 gcagtatctc tttacttttt taatgaacct gacttcgttt tatatagaaa atggtcggtt
     7981 gaaaaaggca cgtagtgtga atgaagatat gaaacggagc ttagcacata aagaaagaag
     8041 tacagtgatc tatgaaacat tgatggcaga ttattatcaa agattgattt ctgctgcttt
     8101 aggggaagaa aagacgaaag attaccaaga attcttcaca gtgctcgaat atatgttagg
     8161 aaagaatcaa cggaggtcct tagagaaaaa actgctggcc attgctgaac ccagcaacta
     8221 gaccagcagt ttagaatttg tatcacttcg ccgctttttc agacggtttg tttatatcga
     8281 atggcgggaa aaagcgggcg atctctttta ccgcagcatc acgggaatca gaagcgtgaa
     8341 tgatgttttc agtccccggc agcgcgtagt ctcctcggat cgtacctgga acagcatcgg
     8401 cagcttttgt tgctccaacc atcttacgga cgatatcgat cacttcctcg ccgaccagca
     8461 caagataaac gacaggaccg gaagtcatgt aatcgatcag ttcatcaaaa aaactgcgct
     8521 cagtcaagtg ctgataatgt tcttttgcta attcaggagt catagtttca aacttcattt
     8581 cagcaatggc cagtcccttt gcttcaaaac gttggatgat gcttccgact aaatggcgtc
     8641 tcacgccgtc tggcttgata ataacaagtg ttctttccat aacagcacct cgtttcctac
     8701 tatatttcta ttatagcggg aggtggaaaa tgagacaaat aaaaccgcgt gagacaggaa
     8761 tataacgtgt gtattttaaa aaaagtatga aaaacatgga aaattgattt gacatcctct
     8821 tttttagttg ttatattaac gaaggaaaac cgaataagat ttgttaggtg aggctcctat
     8881 acaaacatag gctactgccc aaaaacgtcg agagacgcca atgggtagaa caggaattgt
     8941 cgagataagg cttttcataa ggtagctaaa agcaggtatc tgctttttac gttgtacagt
     9001 gtcaaaactc aacgatgaga tctgagaagc tttttttaat atgctttttc aaacctttcg
     9061 ctgagtattt ggcgagaggt ttttttgttg ccgcaaatcc ccatcggtga aaaataaaaa
     9121 ggaggtggtc taattggaaa aataactatt cgaaaaatga ttggaagtca tgaattaaaa
     9181 ttacttagta aaggagatga gaacaaatgg atgacagtca ttcgaaaaac aaggatctag
     9241 aggaaaggcc agggctatta tctatctgtt ctcagcccct ttcagttttt tctctatcga
     9301 tccgatgaat agctaaagac caaaagcagc gagaacagct gtcttttgga cacaaaggag
     9361 agattgagat ggaaaaaaag aaagatacac aaaaaatcta tgcagctttt gctggaaaaa
     9421 cgaaacaaga attatttgaa ctattccatg catcagcaaa tggattgaca gaagaacaaa
     9481 tcgaacgttc aagagaaaat tacggtgaaa atgcgatttc atatggcaaa aaaacaccat
     9541 tcattgtaga aatattgaaa gcatacatca cgccatttac attagtgttg atcgcattag
     9601 gaattatttc attcatcacg gaatatgtat tagcagctcc tggagataaa gatcttttcg
     9661 gtgtagccat tatcttcact atggttatcg tcagcgggac aatgacgttg attcagtccg
     9721 tacgttccaa tcaagcggct gaaaaactga aatcacttgt gaaagtaacg gcagctgtaa
     9781 aacgtaatgg aaagtacaca gagattccta tggaagaaat cgtctgtgga gatcttgttc
     9841 ggctttcagc aggggatatg ataccagcgg atatgcgttt attgtccgca aaggatttat
     9901 tcatttccca agcagcattg acaggggaaa gctatccagt agaaaagcat gcgaaagtat
     9961 gtgaagacga tcagcaaagc gaaacaagtt acgaaaatct tgtctttatg ggaagtaatg
    10021 tcatcagtgg aagtgcggaa ggaatcattg tttctgttgg aaatgaaaca ctatttggtc
    10081 atgtagcaga aacgctatct gaaaaaccaa ttcaatcaag ctttgaatta ggcatccata
    10141 aaacatccat gctactgatc aaatttatgt cattgatggc tccagcagtt atcgtcatca
    10201 atgggcttac aaaaggggat tggctagaag cttttctttt tggactatct gtagcagttg
    10261 gtctaacacc tgaaatgctt ccaatgatcg ttacgacgaa tttggtcaaa ggagcaagtg
    10321 tgatggccaa aaaaggaaca gtgatcaaaa atttgaatgc catccagaac tttggtgcaa
    10381 tcgatgtgtt atgtacggat aaaacgggga cattgacaca agataaaatc attcttgaat
    10441 atcatatgga ttgtgaagga aatgaagatg atcgtgtttt gcgccatgcg tatctaaata
    10501 gctactatca aacagggctg aaaaatttgt tagatgtggc tatcattgat gaagcaacag
    10561 aaactttaga tacagataag atcaattatc aaaaagtgga tgaaattcct tttgattttg
    10621 aacgtcgtcg tatgagtgtt gtggttgctg atccagctgg taaaacacaa atgatcacaa
    10681 aaggcgcgat tgaagaaatg ctgaacattt ctaaatttat tgatcttggc ggtactgtta
    10741 caccattaac aaaagaacga aaagaagccg tacttgcaaa agtacagtca cttaatgaag
    10801 atggcttacg agtgattggt gtcgcacaaa agaccaatcc aagcgtcgtt ggagaatttt
    10861 cagtcaaaga tgagtcagac atggttttga ttggttatct tgctttcctt gatccaccaa
    10921 aagaaacaac gaaacaagcg ttgaaagctc tgaaagaaca tggtgtagct gtaaaagttc
    10981 tgactgggga caacgaatta gttacacgtt cagtgtgccg tcaagtaggt ctagagatca
    11041 atgaattggt taccggtgag aaaatctcgg aaatgagtga tcatgaatta gcacaagtag
    11101 ctgaagacca tgaagtattc gtaaaattga atcctcagca aaaagcacgt ttgacgacag
    11161 ctttacgtaa aaatggtcat actgtcggat ttttaggaga tggtatcaat gacgctcctg
    11221 caatgaaagt ggctgatatc ggtatttctg tagatacagc tgtggatatt gcgaaagaat
    11281 ctgcagacgt gatcctttta gaaaaagatt tgacgatttt ggaaagaggc cttctttctg
    11341 gacgagaaac ttttgggaat atcatgaaat atatcaaggc tacagcaagt tctaattttg
    11401 gcaatatgtt ttccgtgctg attgcaagta cgttcctgcc atttttacca atgctgcctt
    11461 tacaactttt gtttttgaat ctgatctatg atgtttcctg catttccttg ccatgggatc
    11521 ggatggataa agaatatttg gacgagccaa aaaaatggaa tgcatctagt attggggaat
    11581 tcatggttta ctttggtcca acaagttcga tatttgatat tactacttac ttgttgatgt
    11641 attttgttat ttgtcctgct gtagtgggcg gaagtttcca tacattggat agttcacagc
    11701 gagtgatttt catcgcatta ttccatgcag gatggtttgt cgaatcacta tggacacaaa
    11761 ccttagtgct ccatgcactg cgtacaccga aagtaccatt tatccaaagc aacgcaacat
    11821 ttgccatgtt tgttatcact acattaggaa ttattgtcgg ttctgtcctt ccttttacaa
    11881 gttttggtgc tgaattaggc ttgatgccac ttcctggcaa ttactggtta tggttatttg
    11941 ctacagtcat cgcttatctg gcgcttgtga cagctgtgaa gaaaatctat atccgtcgat
    12001 tccaagaact cttataaacg agaaggctgg ttctttgctt ctgttctttt tggaccagtc
    12061 gtgctaaact atgtgtattg ttttggaaag gattaatgta tagatgaaag gtgcaatatt
    12121 tgatttagat ggtttgttag ttgacacaga aaaaacgtat cgtgatggct ggctttgggg
    12181 atttcagcaa tttgatatcc atatcccaaa agaaatcgtt gacgcttggg gcggaaaaaa
    12241 ctggaaacaa agttttgata ttttagtgaa agcagcaggt agccctgaaa aagtacagga
    12301 agtccgagcg aagcgtgaag aatattttta tcaacagctg acaagcggag ccatccactt
    12361 aaaaccatat gcaaaagaaa tattggcaga cttaaaagaa cgtcatatga aactaggcat
    12421 tgctacgacc actgttacga aaagagcaac agatatcttg gatcacttta aattagctga
    12481 ttacttggac acgtttacat tcggagacga agtgtcggag aacaagcctt caccgattcc
    12541 ttatctgaca gctttggaac gaacaggatt agaagctagt tcagcctttg cagtggaaga
    12601 ttctttagta ggagcaacag ctgcttcacg agcaggatta ggcgttgttt tgatacctga
    12661 ttccagtttt gtccgaaatt ataccgtcga agaaaaggaa aaattgaaac ttcttgcgga
    12721 aggaaaagat cttcgaactg ttattgaaat gttaaataaa aaaacagaaa aataaatata
    12781 gaaaaaataa tttgaatttc gtacttgact t
//
LOCUS       DAXWLF010000072        12666 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXWLF010000072 DAXWLF010000000
VERSION     DAXWLF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.072"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACGE4U_001523"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACGE4U_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFF8393694.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="ACGE4U_001524"
     CDS             complement(481..675)
                     /locus_tag="ACGE4U_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393695.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(901..2103)
                     /locus_tag="ACGE4U_001525"
     CDS             complement(901..2103)
                     /locus_tag="ACGE4U_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8393696.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(2106..2300)
                     /locus_tag="ACGE4U_001526"
     CDS             complement(2106..2300)
                     /locus_tag="ACGE4U_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFF8393697.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(2347..3195)
                     /locus_tag="ACGE4U_001527"
     CDS             complement(2347..3195)
                     /locus_tag="ACGE4U_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFF8393698.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(3400..3750)
                     /locus_tag="ACGE4U_001528"
     CDS             complement(3400..3750)
                     /locus_tag="ACGE4U_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393699.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3928..4791
                     /locus_tag="ACGE4U_001529"
     CDS             3928..4791
                     /locus_tag="ACGE4U_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8393700.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            5415..6407
                     /locus_tag="ACGE4U_001530"
     CDS             5415..6407
                     /locus_tag="ACGE4U_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8393701.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            6423..7307
                     /locus_tag="ACGE4U_001531"
     CDS             6423..7307
                     /locus_tag="ACGE4U_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8393702.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            7356..8963
                     /locus_tag="ACGE4U_001532"
     CDS             7356..8963
                     /locus_tag="ACGE4U_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8393703.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8964..10448
                     /locus_tag="ACGE4U_001533"
     CDS             8964..10448
                     /locus_tag="ACGE4U_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFF8393704.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            10625..11617
                     /locus_tag="ACGE4U_001534"
     CDS             10625..11617
                     /locus_tag="ACGE4U_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8393705.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            11669..12538
                     /locus_tag="ACGE4U_001535"
     CDS             11669..12538
                     /locus_tag="ACGE4U_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8393706.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         3921 a         2106 c         2596 g         4043 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg
      781 cattccccat ccatattata tttacgtctt attcccactc aatatgagta gcctcgtaaa
      841 gaagctatta tatcagtgct ttttcagcct attcctcatt ttggtaggga aatggtaggg
      901 ttagattttt actgcttgat ttccattaaa gtttgcaact ttagattttg ttgcacaggt
      961 aaatttaatg tttcctgtca tttgttctgc aattccttca tcattacggc tccatagatg
     1021 agcgtagtgc tttaaagtga tttcagggct agaatggcct aaccgttgcg acacgaccag
     1081 aatgtctgcg ttaaactcat tgattaggta gctaacatga gaatgacgta atccttttcc
     1141 ttgtattagg ggtaccttag ctagtttggc ataacgctga ataattcgtt gaacagttga
     1201 acgatagagt gggaggtcag tgtagctaag aataaattgt tctacaccat gatttttttg
     1261 aataactttc cactttttta gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa
     1321 gccgctttca gtttttgtgt aaggtttccg aataaaatct ttttgatttg tcatttccaa
     1381 ggtgtgatga accctcaact ttttacgttt gaagtccacg tcattccaat taagtgctaa
     1441 tccttcgctt acacgaacac cagtcatgta atatagccag atcattaaaa aacacatatg
     1501 ttcgtagaaa tcatctttat aaatttttgc caaaacttct tcaaattctt cttttgtcca
     1561 gtaaggaacg attgattttc cttttggaat tgcttttgtt cttttggaaa tattctcatt
     1621 cagaaattgc agggtgaccg catagtctaa tgatttacga aacataccgt acataagtga
     1681 actgtatgct tgtgagtatc cgctatcgtt caataaccaa attcgataat tttcacaatc
     1741 tcgcacattg atttcacgaa gcttcttatc accgaaaaat tcagtaatct gaatcaatcc
     1801 acactcacga gaactccaag tacttttttg tacgctagct ttgtagtgtg gaacgtaaga
     1861 ctgctccata aattctttgt aagttaaatc ataattcgca aatccattta caaccatgta
     1921 ttcatttttt atacgcatga cttctttgtg tgcttcacga gctgatgaga atcgttttcc
     1981 tagagaatct tttgttcctt tcttctggat cctctttcca gtcacttgat ctgttcctaa
     2041 ctcaactaga taagaaaagc gaccattttt atcttgatac acacaagggt attttgtttt
     2101 catagttatt cctcctgtga aagaggaaca cctataattt cttcaattac ttgtcggggt
     2161 actaccccaa ctcttttatt attataatat ggatatccct gatttaccat tcttgttttt
     2221 gcttgtcgga ttattgtggt agcttgatgt gttttaaatc ctagagcctc taaatctttt
     2281 cgacaaattg tatttttcat tgtataaact cactttcaaa gaaaacacta tcattgggta
     2341 atagtgttat ttctttattt ttgtataatg aacggtactt tcttgaaaat ccatcacttg
     2401 ttcaggactt cccaactcat ttaaagctaa agcattgtaa aaaaatggac gtctaactat
     2461 atattcgctt gtatatagta tatcttgatt cgtggaatca tagggtttct ttaccatctt
     2521 tttggtgaca gttggtagtt tgagattccg ggacttgaaa aaagcttttt tcttatgctc
     2581 tttcaattcc agctctttat cgaaatattt gctgatatag cgcccgcgat tttcgatact
     2641 gtctacatcg atcttgttga tcttaacgaa accctgaccc caaatcgtag ttaaccggtt
     2701 aaatgagata aaggggaaat caaacaagat aatatggtag tggattgcac cgcgttgctg
     2761 cttttcccac gtggcgagat actttaaatt tgctttttta gttttataga ggtcataatt
     2821 cagtcgtttg ataaattttt taaattcatt atttgcatac tcaatgtctt gaatgttttc
     2881 tttgaaagtg agagttaaaa atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc
     2941 aatctcaaat cgcatacttt gataatgttt ctgtttgcgt tttaaactgt catattgtcc
     3001 ttgagcggat aattcatcat agttttttct tttttcaaga gtaatatcat tttcataaat
     3061 ccaagctaat ttttgttttc gttccagatt tacttcgttt tcgatcgttt tacgtttggg
     3121 agtaatgatt gcttcataat cataaatttc aatatacgtg ggagtttcaa tgattttctg
     3181 attataagct tgcaaaaatc atcactcctt gttgcgtatt cgattgtcaa agaaccagtc
     3241 attttctcct gatgttgatc tataaatcaa gttaaggggg aaggcctgcg tagcagccct
     3301 tctagcctat ggggtgttgg gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc
     3361 cgccgtttcg tcctaaccac ccaacactcc atagtatagt tagttttgtg tagtggtacc
     3421 ttgttttctg ggaatcaaag ttgccgtatc agcggttgcc gataacccat aactgacaaa
     3481 ttgtccattc gagcgtcccc aaggtacaat caccaaattg gtaaatgtaa ttatggctaa
     3541 accttgttgg gtaagttctt ccggcgtgat cacaggatgt tcagatttga ctttgacact
     3601 gattttttcg tagcgaagtt ttggtaggat acattcgtag ctccaaccta gaatttcgcc
     3661 ctccttttgc cagcgagtga cattgacgac ttggtattct ttttcgagtg tttcttttgg
     3721 taaactgaga tcggtaattt ttattggcat cacaattctc cttttttata cgaaagttta
     3781 tacgatttga ttaaaaaata tagatggcca tctacgactt gtattatata cgagaactta
     3841 tacgtagtca agagattttc taaaaaaata tatacgattt tttatacgag tgttataatg
     3901 aaaaaaatga taaggaggaa tttgaatatg tttggtttag tacaacctaa taaagtgaag
     3961 gtgggccaac gaatcaaaga aataaaagac ggaatgtctc tctcttttac agaattaggg
     4021 aatcgattag gtttaaaaaa gccgacgatc agttcctacg ttcaaggcta tgcgttggct
     4081 cctaagagtg tgatcaacca attatctagt atcagcggaa aacctgtcgg ctggttttat
     4141 tttgggcatg tggaagaata tatccgagag tatttgtttc tcattggtca gaaaaaaata
     4201 gtggacgatt atcctgaaat cattgagaaa ataaagcaag aattttatac gggcgagttc
     4261 aaaaatcctg cttgggagaa tgagctaggc tatcctttgg aggaatttat ccaagaatgt
     4321 tttgtagaat attcgagtga aatcatgaag gactatgtcg tacagattac gaaagaggta
     4381 cttgaagaat ccaaaacgct aaacaaacta tcatcaaaag caaaagaaga agctgtcacg
     4441 attctttcat ctgggatcat tgactatatc gagatgagtg gtgagatcaa gtatggagag
     4501 aaagatcgca ttgcgaaaat ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac
     4561 ctgacattta atgattccta tttgattgga aagttgatca atattcttgg tacagatgaa
     4621 gatacggagc tgtttctttc ttctttatca atgatattta ctgataagca tttttccact
     4681 cgatttggtg gggaagaatt agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa
     4741 ctctatcggg agaaaacacc agatgaattt tatgattggt ttgagaagta aaataagaaa
     4801 agtggcttag attacaagtt ggtgaaaaaa tttttttcag cataacatca gtaatctata
     4861 aaggggttgt tgattatact gtagaagtta agtactcaag agattttttg tacgtaaaca
     4921 aagcaaaaaa gggtgcagtt tcactagaaa atgtactctt ttttgtgatt tttcttagtt
     4981 atagagtttt tttgaaactt taaaaatgag aaaacatatt gattttaaag agtgtagaca
     5041 tagcttcttt tgctaaatag attaaactta ctggtgagtg gtcttgaaca tacttttaaa
     5101 tcacatttta ctgagtttta ttggatttta taattcattg gaaacctcgt acttaatctg
     5161 tttttatcta tcgactagaa tctgtatata ttccagctat caatgaggcc gcagaaaagg
     5221 gtgcttaaaa ataatctgtt gtttaaaata ttattatcag aatacttaga tacaaaattc
     5281 tggcacaaag taaaaaaaat attgacttaa aaaaatggat atggtaatct ataaacgaaa
     5341 atagtggtaa cgtttccaaa atgcatcttc gttaaatttt tattttttta aacgaaaatg
     5401 gaaacgtttc taaaatgtat tgggaggaga ttgaattgga aaagacattg ggaccaggtg
     5461 gtgagataag ggcggtacaa cgggataaaa aaaatatttc gaagtgggaa aaattaaaga
     5521 aacagttgcg gcgtgataag tggatgtact tgttgctctt accaggtatc atcatgacgt
     5581 ttatctttaa gtatgtgcca atgtacggtg ctgtgattgc atttaaagac tataatccta
     5641 tgctaggaat tttggaaagt ccttgggtag gactagatca ctttagggaa tttatcagtt
     5701 caccaaattt tacgcttttg ctgagcaaca cattaaaaat tagtatttat ggtttattgt
     5761 taggtttttt tccaccaatt attttagcac tgagcttgaa tcaaattatc aataaaaaaa
     5821 taaaacagaa aatccagttg attctatatg cgccgaattt tatctcagta gttattattg
     5881 tggggatgct gtttttgttc ctatccgcaa atggacctat taatgggtta gtagaaaagg
     5941 ttacggggca accaattatg ttcatgtcta atccagctta tttcagaacg atttatattt
     6001 tatcgggaat ttggcaagga atcggctggt catccattct ttacaccgca acgctggcgg
     6061 gagtcagtac cgatttatat gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga
     6121 tttggcatat cgatttgcct gcattgaggc ctgtgatggt gatcagcttc atcttggccg
     6181 ccggcggtat tatgaatgta ggatatgaaa aagcctactt gatgcaaact tcaatgaaca
     6241 tccctgcctc agaaatcatt tccacttatg tatacaaagt cggcttgcaa tcaggtgatt
     6301 atgcctactc tgctgctgtt ggtctgttca atatgattat taatgtcgtt ttattactga
     6361 ttgtcaacaa tataaccaag cggctaaacg atggtcaagg cttgtaatga aaggagaaaa
     6421 ttatgaatag tgctttatca aaaatcgacc gtcaaatctt atggttgaat cgagtgattg
     6481 taactttttt agtcgtcatc accattgttc cgctgattta tgtactggta gcttctttta
     6541 tggacccagc tactttgatc aataaaggta taagcttcaa tccgaaagat tggacaatac
     6601 agggatatca acgggtattt gcagatgatt ccattttacg cggttttgta aattcgttgt
     6661 tttactcttt tagcttcagc ttattgactg tatttattac gatgatcact gcgtatcctt
     6721 tgtctcgacc taacttggcg gggaaaaaga ttattatgac tttttttgtc attacaatgt
     6781 tcgtgggtgg tggcttgata cctacctatt tattggtaaa gaacttgggc atgttagata
     6841 gtgtatgggc gattatcgta ccaggtgcta taaatgtgtg gaatattatc ttagcccgta
     6901 cctattttag tgctttgcca aaagaacttt ctgaagctgc tactatcgat ggcgccaatg
     6961 acttgcaaat actgttcaaa gtcatgcttc cattagcgaa accaattatg ttcgtattgt
     7021 ttttatatgc ctttgttggg caatggaact catactttga tgccatgatt tacatcaaag
     7081 atcccgactt acagccgttg caactcgtat taagaaatat tttgatccaa aaccaacctt
     7141 cacaagacat ggtgggagcc aatactgcca tggcagaaat gaaacaaatt gctgagttga
     7201 tcaaatacgc aacgatcgtc atttctagtc tgccattaat cataatgtat cctttcttcc
     7261 aaaaatattt tgataaaggt gttttagttg gttctgttaa agggtaagta ttgttcacta
     7321 tttgtatgaa taaaaattat aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa
     7381 tcgtttttac ttccgtacta attttggggg cttgtggagg ctcaaaagaa aagagtaaat
     7441 caagccctga ttatgaacta gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa
     7501 aaatgatgtc tcaaagtgcc cctttggctc ccaatgatcc taatgacaaa ttgattttcc
     7561 aacgattaga agaagagacc aatctccata ttgattggac aaactacagt agtgattttg
     7621 ccgaaaaaag aaatttggat atcgctagtg gtgatttacc agatgccatc ttcaacgccg
     7681 ctgcaggtga ctatgattta ttaaactggg cggaaagtgg cgttattgta ccgattgaag
     7741 atttgatcga agattatatg ccaaatctga agaaaatttt tgatgaaaac cccgaatacc
     7801 gacaaatgtc tactgcacct gatggtcaca tttactctat gccttggatt gaagaattag
     7861 gcgaaggaaa agagtcgatt catacagtaa atgggatggc ttggatcaac aaggagtggt
     7921 tggataattt gggtcttgaa atgccgcaaa ccactgatca gttgatgact gtgttggagg
     7981 ctttcaagac gcaagatcca aatggtaatg gtgaagcaga tgaaattcct tttagcttta
     8041 ttaacaatgg aggaaacgaa gacttaaaat ttttaatggg ggcattcggt ctaggtgata
     8101 atgatgacca cttagtcgtt gataacgatg gtcaggtaca atttactgct aacaaagaag
     8161 ggtataaaga agccatcaaa tatttcaatg aaatgaataa taaaggtctt attgataaag
     8221 aggctttcga acaagattgg aatacgtatg ttgccaaagg gaaagaacat aagtatggcg
     8281 tgtactttac ttgggataaa gcaaatatca cgggtgcaga tgatagttat gacgtcttgc
     8341 cagtcttagc gggaccagat ggaacgaaac atgttactcg tacgaatggc atgggtttct
     8401 caagagatcg tttcgtaatt actagtgcga ataaaaactt ggaacttaca gctaggtgga
     8461 tagataaaat gtatgaaccg ttacagtctg tccaaaataa ctggggaacc tacggtgatg
     8521 aagcacaggc gaatattttt gaattaaagg atggtatgtt aaaacatttg cctttagagg
     8581 ggacagcacc gggggaacta agacaaaaaa ccgaagctgg aggaccatta gcagttcttg
     8641 attcttatta cggcacagtc actactatgc cagatgatgc gaagtggcgt ttagatttga
     8701 tgcatgacac gtatttgcca tatattacag aagacaatat ctatccacgt gtctttttag
     8761 aaaaagaaga aacagatcga ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc
     8821 gcaaaagagc agagtggatc accggcggta aaatcgatga agaatgggaa gaataccttg
     8881 ctgaattgga acgtgtaggt ttggaagaat ggattcaaat aaaacaaact ggctatgata
     8941 attttttgaa gggagcacaa taagtgttag tagaagaaac ggcgattttg gataaagcaa
     9001 atcgatttat tgaaatgatg cgaggaaaag ttaaccctca gtatcgacaa aactatcatt
     9061 tttccgcacc catcgggtgg ataaatgacc ccaatgggtt tatatattac aaagggcatt
     9121 accatctctt ctatcaatac tatccttacg atagtcgatg ggggcccatg cattgggggc
     9181 atgcgcgcag tagtgatttg gtccattggg aagatctacc agtagcgtta gccccagatc
     9241 aaccttatga cagcggcggg tgtttctcag gaagtgcaat tgaaagagac ggaaaactat
     9301 atttatttta tacaggctgt caagaaattg atggtattag ccatcagcat caatgtttgg
     9361 cggtctctga agatggtatt cattttagca aatacagtgg taatcctgtg gtagcagaaa
     9421 aacagctagg tgaaaatggg ctaatacaag aatttcgtga tcctaaagta attgttcaca
     9481 acgatggtta ctatatggta gtagctacta aatctacaca gtctactggt agaatagtga
     9541 tgtttcaatc gtcagatttg tatacttggc acttttactc tgtgctgcta gaagggactt
     9601 cagagcaagg aataatgtgg gaatgtcctg atttattcca tttagacgga cgagatgtct
     9661 taatcatgtc tccgatacaa atcaagaaga acggactgga atatcataat atcagttcta
     9721 ccatggcttg tattggtgag gtaaactggg aaacaggtca gatgatagtt gataatttcc
     9781 atgaaatgga ttttggaatg gatttttatg caccacaaac aacaagagat cctaatggtg
     9841 gacgggtgat gattgcttgg atgcagatgt gggatcgtac attgcctaca catgagttag
     9901 gccatctttg ggcaggctct atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat
     9961 tggtacaaaa acccatctca actgtatata gtcatttagt ttatcaacaa ggcagtgaaa
    10021 atattgcagt gactgacaca tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt
    10081 tacatctagt cgcagatttg gagaaagctg aagtttttac aattgaatta ctgcggtcta
    10141 agacggattg tctcaaacta gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc
    10201 agaaactagg aagagagctt acaggggcgg aagaagaccc tctgaatgta cgaaaagttg
    10261 aagtaccttt gattgataat cagctcgttc ttgagatttt tagagatacc tcatcaattg
    10321 aagtctttat taatgggtta gaagttatga cggctacttt ctatgagctg gaaaaagctc
    10381 gagatatctt gtttcattcg atcggagaaa gtcgtattca agcttttgaa acagcgagtg
    10441 tgaaataaat gtttgattgc ttaggcgatg aatcaattat ttaataggtt ttgaaggata
    10501 atgtttttag aaaagaacag atagctagat ttcccatgga tttgctatta gtattttgaa
    10561 aaaattagta atttatgaca aaattcatta tactaaactt gtgcagaaat gtgagggatc
    10621 aactatgaaa gcaacaatga aagatgtcgc aaaattagca ggtgttggag taggaactgt
    10681 ttcccgggta ttgaataacg gttctgttaa agcttctact agagaaaaag ttgaagcagc
    10741 aatgacagag ttaaactatc aaccagattt ttatgcccgg gggttaaaga tgaagcatac
    10801 gaacacggtt gctttgattc tacccacggt atggcatcca ttttttggcg aatttgccta
    10861 ccatgtagaa acagcattag aaagacaaaa ctacaagctt tatttgtgta actccagtgg
    10921 taatagtgaa aaagaacaag aatatatcca gatggtcagc caaaataaag tagacggcat
    10981 cattggaatt acttattcag atatcgatga atacatttcg agtaatctgc cttttgttag
    11041 tattgaccgt tatttcactg aagaggtgat ttatgtgacc gctgacaatg cggccggtgg
    11101 acgtttggct gcaaatgaac tttatcgccg aggatgtcag aatgtagcat atatcggtgg
    11161 gtaccaagaa acaccgaatg aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg
    11221 tcaactgggg atttcctata aagcgttaga tatgttagaa cctttagacc atctctcaga
    11281 acgtgtaaca aattttttgt tagagaatcc cgatatcgat gggattttta cgatcaacga
    11341 tccaatggca ttagctgtca ttgatatgct tgaaaaaata gggaaaaacg taccagagga
    11401 tgtccaagtt atcggatttg atggtcaaaa gatgacagaa ggagatcctt atctagtctc
    11461 aacgattgcc caacctacaa agcaaatggc ggaagtggca gtggcaaaat taattgctgt
    11521 cattaatgga gagacagtag aaaaacgtac cgtattgccg attgaatttg gcgaaggtga
    11581 cacaacgaaa cctttagtga gaaacgattt tgcttagtgc aaatacgttt ctttttttga
    11641 aaatgagtga atgaaaggtg tagataaaat gttatatggt ggaatagaag ccggtggaac
    11701 aaagtttgtc tgtgggattg gtaatgaaca attacaagtg atagaacgga aaagttttcc
    11761 taccacaaca ccagaagaaa cgatggagca agtgtttgat ttctttgata agtacaaagg
    11821 aaatatcgca tcaattggtg taggagcatt tgggcctatt gatattcaaa tcgaatcaag
    11881 aacttacgga tatatcacca acacaccgaa gttagcttgg caaaattatc attttgtagg
    11941 agctttggaa aacgccttgt cggttcccat ctattggact accgatgtaa atgctgcttg
    12001 ttatggagaa tatatggcag ggtatggaaa aggtaaaaat agtgttctgt attacaccgt
    12061 tggtacaggt gttggtggtg gtgctgtagt ggatgggaaa atagtcgcag gttttagtca
    12121 tcctgaaatg ggacatatgc tcttacaacg tcataatgaa gatcaatttg aaggaatctg
    12181 tccttttcat catgattgtt tggaagggtt agcatcagga cctgcgattg aaaaacgctt
    12241 tggaagaaaa gcccagacta tctcatttga tcatccttat tggacgattg aggcagatta
    12301 tcttgctcag tgtgtgtata acacaacgtt actatatgca ccggaagtaa taattttagg
    12361 cgggggtgta atgaaacagc cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa
    12421 aatgggttcc tacgtggata cgcctactct cgaagaatat ctactaactc caaggcttgc
    12481 tgacaatgcg ggcttgattg gctgcctagc tttagcaata aagaaactca atcattagaa
    12541 tagtatttat tagaactatt cataacatac cggaagttta aaagggtatt ctgaaactta
    12601 aatagtctca attcattctt ctttcttgag aatctgagta gagactttta ttctgtttca
    12661 aattaa
//
LOCUS       DAXWLF010000073        12519 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXWLF010000073 DAXWLF010000000
VERSION     DAXWLF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.073"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            365..1945
                     /locus_tag="ACGE4U_001536"
     CDS             365..1945
                     /locus_tag="ACGE4U_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFF8393707.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            2143..2367
                     /locus_tag="ACGE4U_001537"
     CDS             2143..2367
                     /locus_tag="ACGE4U_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFF8393708.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDLKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(2572..2892)
                     /locus_tag="ACGE4U_001538"
     CDS             complement(2572..2892)
                     /locus_tag="ACGE4U_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFF8393709.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(3040..5274)
                     /locus_tag="ACGE4U_001539"
     CDS             complement(3040..5274)
                     /locus_tag="ACGE4U_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8393710.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            5753..6019
                     /locus_tag="ACGE4U_001540"
     CDS             5753..6019
                     /locus_tag="ACGE4U_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFF8393711.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            6019..7746
                     /gene="ptsP"
                     /locus_tag="ACGE4U_001541"
     CDS             6019..7746
                     /gene="ptsP"
                     /locus_tag="ACGE4U_001541"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFF8393712.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            7910..8956
                     /locus_tag="ACGE4U_001542"
     CDS             7910..8956
                     /locus_tag="ACGE4U_001542"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFF8393713.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            8973..10196
                     /locus_tag="ACGE4U_001543"
     CDS             8973..10196
                     /locus_tag="ACGE4U_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFF8393714.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            10285..11172
                     /locus_tag="ACGE4U_001544"
     CDS             10285..11172
                     /locus_tag="ACGE4U_001544"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8393715.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            11258..11488
                     /locus_tag="ACGE4U_001545"
     CDS             11258..11488
                     /locus_tag="ACGE4U_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFF8393716.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(11545..12510)
                     /locus_tag="ACGE4U_001546"
     CDS             complement(11545..12510)
                     /locus_tag="ACGE4U_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFF8393717.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
BASE COUNT         4076 a         2180 c         2553 g         3710 t
ORIGIN      
        1 aaacaagcaa cacgtctttt cttttagtca gctgacaaaa aaatatttga ctcttatggt
       61 ttggtggctt tttcattcga tgaatccatg ctatactgat tcagttgtag tagttgacaa
      121 aggttgtgaa aaagctatcc tgcattaagt aataagagca gccaagcaaa aacagctttt
      181 cgtatttttg gcttgggtgg gcttattgct gataggctgt ctctagaacg ccgtttactc
      241 ggaaaaagag actgctacac ggcttttgtc acagccctat atgaaattgt ctttctttaa
      301 gagatcggac aatctgtgcc tagagaaaat ataaataatt gaatggaaga aatggagtta
      361 ggttatgaat aatccagaat taaagcaaca agtggacaat cgcagaactt ttgcgatcat
      421 ttcccatccg gatgctggga aaacaacgat tacagaacaa ttactattgt ttggtggggc
      481 tatccgtcaa gccgggaccg taaaaggcaa aaaaacaggt aattttgcca aatcagactg
      541 gatggaaatc gagaaacaac gtgggatttc tgttactagt tctgttatgc agtttgatta
      601 tcaagggaaa cgagtaaata tcttggatac accaggacat gaagattttt ctgaagatac
      661 ttatcgtact ttgatggccg tggatagtgc ggtcatggta atcgatagtg caaaagggat
      721 cgaagcacag acaaagaaat tattccaagt tgtaaaaaaa cgtggcatcc cgatatttac
      781 ttttatcaac aaactagatc gtgatggtcg tgaaccattg gaattgttag aagaattaga
      841 agagctatta gatatcgaat cttatccgat gaattggcca atcggtatgg gtaaagggct
      901 agaaggtctt tatgatatct accataatcg tgtagagttt tatcgtccgg aaaattatca
      961 ggatgaacga ttagtagaat tggatgaaga tggactgctt cccgaaaatc atcctctaac
     1021 tgaaaattcc ttatatgaac aagtcttaga agaagtagaa ctaatcaaag aggctggaga
     1081 tgcatttaat caagaaaaaa ttgctcgtgg cgatcagaca ccagtatttt ttggttctgc
     1141 attgactaac ttcggtgtac agactttttt agagactttc gttgaattgg caccagctcc
     1201 ttatggacac aaaacacaag atgatcagat ggtaagtcct tatgaggaag aattttcagg
     1261 tttcgtattc aagatccaag caaatatgaa tccagcccat cgtgaccgaa tcgcttttgt
     1321 ccgaatctgt tctggaacat tcgaacgagg aatggatgtc tggttggaac gtacaaataa
     1381 aaaactgaaa ttaagcaatg tcacacaatt tatggcagat tcgagagaaa acgtcgaaaa
     1441 agcagtagca ggcgatatca tcggtgtata tgatacaggc aattatcaga ttggggatac
     1501 attgttcgaa ggaaaactaa aagttgctta tgaagaactc ccttcattta ctccagagct
     1561 gtttatgaaa gtgactgcaa aaaatgtcat gaaacaaaaa tccttccaca aaggaatcta
     1621 tcaattagtt caagaaggag cgattcagct ttataaaaca tatctaacgg aagaatatat
     1681 cattggagct gttggtcagc tacaattcga agtattccaa tatcgtatgt taaatgagta
     1741 taacgccgaa gtaatcatgt ctccaatggg acacaaaatc gctcgctgga tcgatccaga
     1801 agatttggat gagaagatga gttcaagtcg caatatcttg gctcgtgacc gtttcgacca
     1861 accattgttc ttattcgaga accaatttgc agaacgctgg tttgcggata aatatcctaa
     1921 tgtaaaatta aaaagtttga tgtaaagaag tcttgacaca agctgccctg catcaagtaa
     1981 taagaatagc caaacaaaaa cagtctctca tattttgttt agagccgtga cggattttcc
     2041 gtgacggctc tttttactat attaaaatca tagaaatgtc taatcaaaaa aatataaaaa
     2101 ttattttgcg tacatatgat taaagaaagg agagtgtgac acatggaaac agaatggcaa
     2161 atacgtgaaa gaaaaaaaca gaaaaaagaa gagatgttac gattatgtcg attattagat
     2221 ttaaaagact gggacaaaaa cgagcaaatt gcacaaaaag tacgatcaat catccataaa
     2281 gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa atgaaaagag aaaagtacaa
     2341 aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg ctgagacaga agcgtttaac
     2401 tccaagaaat aagctggaat tcacaaaaat ttgaaaagca attttcgtga attttagctt
     2461 atttccgaag gagttgcttc tgcttccgcc gtttatacgt gtttagagcg tgagacaaaa
     2521 gtgttcttta cttttgtccc acgctcttta attattatgt tcaaaatttg tttacacggc
     2581 taatgtagcg cctaattcat gttttggaat cgagatttca acaaggcctg ccaattcgat
     2641 tactttgatt tcatcctcgt ggtaaacggc gtctttgatt ttatctaaga aataattttt
     2701 agcatcttcg atttctttcg gctggatttt acgtttgtca ttgatcagca gaatttcatt
     2761 atgttcaggg gcttcagtgt agacaatagt ggtatttccg gctgtgtaga ccttgactag
     2821 atgggcatca gtgttttcaa gctggtttaa tacaagacgg gaatggctgt ttgttacatt
     2881 taccaatttc atggcgctca cctcttttat gtatttttaa ttgcttgttt ctcttattat
     2941 aatttctttt ttataaaatg aacagtattt tgacttttct gaaaaaagaa tgagacggca
     3001 tttctcaaag aaataccgtc tcacgctata aaactggatt tattcagccg tttcttcggc
     3061 tgattcttta gccaaacgtt ctggtttaga agtaacgatg atttttccat ctttatccag
     3121 ttttgctttc aactcatgga tagaagggtg atcaagataa aattcagcga taccatcttc
     3181 gatttgttct tggatcgtgc gtcgaagagg acgagcaccc atagatggat catagcctaa
     3241 gtcaactaat ttttctttga cattcgtagg aacttcgatg tgaagttgct gtgctgccag
     3301 catttggttg acatcgtcta gcattagtgt gacgattgtc atcaagtttt cttttgataa
     3361 tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat tctggtgtaa agtagttgtt
     3421 taattggttc aatacagaac gagtaacacc ttcgcgtgcc gcaccaaaac caacgtttgc
     3481 ttctacttta ccagttccgg cattacttgt cataatgatg atcgtatctt taaagctgac
     3541 cgttcttcct tgtgcatctg tcaatcgacc atcatctaag atttgcaaga acatatgcaa
     3601 aacatctgga tgagcttttt ctacttcatc taacaagaca agactgtaag gatttctccg
     3661 tactttttct gttaattgac cagcttcttc gtatccgaca taacctggag gagaaccaat
     3721 caatttagag acactatgtt tttccatata ttcagacata tcgaaacgaa tcattgaatc
     3781 ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc tctgttttac ctacaccagt
     3841 tggtccaaca aataagaagg agccgattgg acgattctgt ttgtttaatc caacacggtt
     3901 tcggcgaatc gctttcgcta ctcgatctac agcgttatct tgtccgatga catgagcttt
     3961 caagtcatct gctaagtttt tcagttgtgt ctgttctttt tcttttaatt cacccactgg
     4021 aatacctgtc cgttgttcaa cgattttttc catatctttt tcggtaatca caggtgtttc
     4081 ctcttcagtg agctgacgtt ctttcatttt ttgaagttta ttgatctgat cgcgataata
     4141 agctgctttt tcgaaatctt cttctcgaga agctaaaact ttttgttctt ctgcttcttg
     4201 cagttttttc tcgattgttt taggatcaac gatttggatc gtcaagtttt tcttagaacc
     4261 tgtttcatct agcaaatcga tggctttatc cggcaagaag cgatcttgga cgtaacgatt
     4321 ggataaagtt gcggctgctt cgatggcctc atcagtatat tttacatgat gatagtcttc
     4381 ataacgtttt tgcaaacctt tcaaaatact gatcgtttct tcgacagttg gttcatctac
     4441 ccgtacaggt tgcatacgac gttctaaagc agcgtctttt tcgatgatgc ggtactcatt
     4501 taaggtagta gcaccaacca tttgcaattc tcctcgagct aaagctggtt ttaaaatatt
     4561 accagcatcc atatttccat caccagcaga accagcaccg acgatttcgt gaacttcatc
     4621 tatgaatagg atcacatttt ctgcttgacg gatctcttca atcaattttt gcatacgttc
     4681 ttcaaattgt cctcgtatac cagtaccttg gactaaagat acgacatcta aacgaatgac
     4741 ttctttgtcc attaattttt gcggtacatc accatctacg attttttgtg ctaaaccttc
     4801 aacaactgct gttttaccaa cacctggttc tccaatcaag acaggattgt tcttggttcg
     4861 gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg cgtccgatca caggatcgat
     4921 atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat tctcccaata aaccttctgt
     4981 attgccttga ggtggctgac caccattgaa gttgttgttt ccgccaaatt gtgttggtgg
     5041 tgtttgctca taatttcctt gctgttgcat ttggcgagac atggaacgga aaagatcgtc
     5101 caaactgcca aatccaaatg gatcattttg ggccatatta gtcagacctc cattttgttg
     5161 atttttgatt ttttgataac agctctgaca gtaatcgagt tgtgtccgct gaccattgac
     5221 agtggcatac aagtgaatcg ttgcctcatt cttgccgcag ttttgacaaa gcatgaatca
     5281 ttcacgtcct ttcacaaaaa tgggtacatc catattttac atctcgtttt tcatgaggta
     5341 aaagaataac ccctagtttt ttaagagatt tatgaggtag aaaaggtgaa atctctattg
     5401 accattcctg acctttgact cttattatac taaaaataaa aagggaagca agtaattcgt
     5461 ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa acgtcaggag aagagaaagc
     5521 gacaagaaaa agaaaataaa cacgtttgtt ataaaatgtg aagaaaagga tgaaaaagaa
     5581 tccggaataa tttgaatact tttatctgaa attgaatgaa aactgttgta tctaacgaaa
     5641 aaaaatgtta atctttacag atgaaagggt ggcatttgtt aggattctct gaattactaa
     5701 aaaagaaaac cctcacataa aaacaacact cacttaaagg agaccgatta atatggaaaa
     5761 gaaagaattt cacgtagtag cagaaactgg gatccacgct cgtccagcta cactattagt
     5821 acaaacagca agcaaattta actctgatgt aaacttagag tataaaggta aatcagtaaa
     5881 cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt caaggttctg atgttactat
     5941 tactgcagaa ggtgctgacg aagctgatgc aatggcagca atcgttgaaa caatgaagaa
     6001 agaaggctta tctgaataat ggttgaaatg ctaaaaggga ttgccgctag tgacggtgta
     6061 gccgttgcaa aagcttacct gctagttcaa ccggatttaa cattcagtaa ggcaacagta
     6121 gaagatactg cggctgaaga agctcgtttg gatgctgcat tagctaaatc tactgaagaa
     6181 ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg aagcagaagc gcaagtattt
     6241 gatgcccatt tgatggttct ttcagatcca gaaatggtag gtcaaatcaa gcaaaatatt
     6301 aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag taacggatat gtatatcggt
     6361 atgttcgaag caatggaaga taatgcttat atgcaagagc gtgctgcaga tattcgtgat
     6421 gttgctaaac gtattttagc gcacttatta ggcgttactt taccaaatcc atcaatgatc
     6481 aacgaagaag tggtcgttgt tgcacatgac ttgactccaa gtgatacagc acaattagat
     6541 cgcaattttg taaaagcgtt tgtaacagac attggtggac gtacttcaca ttctgctatc
     6601 atggcacgtt ctttagaaat tccagcaatc gttggtacaa aagaaattac tgctaaagtc
     6661 aaagaaggcg atattttggc tgtcaacggg atcgaaggcg atgtaatcat cgatcctaca
     6721 gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt acgcagcaca aaaagcagaa
     6781 tgggaaaaat taaaaaatgc agaaactgtc acagctgacg gcaagcattt tgaattagct
     6841 gcaaacatcg gaacaccaaa agatctagtt ggtgtccata ataatggtgg agaagcagtt
     6901 ggtctgtacc gtactgaatt cctgtatatg gattctccag acttcccaac agaagatgac
     6961 caatatgaag cttacaaagc tgtattagaa ggtatggaag gaaaacctgt agttgttcgt
     7021 acaatggaca tcggtggaga taaagaacta ccatatctgc aattacctca tgaaatgaat
     7081 ccattcttag gatatcgtgc attgcgtatc agtttatctg aacaaggcga cgaaatgttc
     7141 cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg gtaacttacg tatcatgttc
     7201 cctatggtag ctacactcaa agaattccgc gcagcaaaag cgatctttga agaagaaaaa
     7261 caaaaattga tcagtgaagg caaggaagta tctgatacga tccaagtcgg catcatgatc
     7321 gagattccag cagcagcagt cttagcagac aagtttgcaa aagaagtaga tttcttctct
     7381 gtaggtacga acgatttgat tcaatacaca atggcagcag accgtatgaa cgaacgcgtt
     7441 tcttacttgt accaaccata taacccatca attttacgtt tgatcaaaaa tgtaattgat
     7501 gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag aaatggctgg agatcaaaca
     7561 gctgttccat tgctagtagg tatgggtcta gatgagttct caatgagtgc gacatctatt
     7621 cttaaaacgc gtagtttaat gaaacgcttg gacacagcga aaatggctga acttgctgat
     7681 cgtgcattga aagaatgcga tacgatggaa gaagtcgttg aacttgttca tgaatatgta
     7741 aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag agctacgcca gtttttttgt
     7801 tcttttttaa gaaaacgttt ttgtcagtaa gaatagataa aaatagggct taagtttgag
     7861 tgacagtttg taggctatgt tatactatat aagtgaaggg ggacgtaagg tgaaggtatt
     7921 attatatttt gaaagtgaaa agatgcttgc aaaatctgga atcgggcgtg cgcttgatca
     7981 tcaaaaacgt gctctgacgg aagtcggtat ctcttataca ctagatgaaa aagaagatta
     8041 tgacatcctg cacatcaata catacggaat caatagtcat aatatggtca ataaagccag
     8101 aagagaagga aaaaaagttg tctatcatgc gcattctaca gaggaagatt tccgaaattc
     8161 ctttatcgga tcgaaccagc tttctcctat cgtaaaaaaa tatctggtag gattgtacca
     8221 aaaagcggac tatctgatca cgccaacacc ttattcaaaa cagttgttgg aaagctatag
     8281 tatccgtgtg ccgattcaag ctatttccaa tgggatcgac ttagagaaat atcgccctga
     8341 tccaatgaaa gaacaaaagt tcagagaata tttcaaactg tctccggatc aaaaagtaat
     8401 catttgtgtc ggtttgtttt tcgaacgtaa aggaatcatt gatttcgtag aaattgctaa
     8461 gaagatgccc gaatacacat ttatttggtt tggtcatgtt cccatgtact cgattcctcg
     8521 gaacatccgg aaaattgtaa aagaagatca tcctgaaaat gtcctgtttc ctggttacat
     8581 tcgtggagag atcatagaag gtgcgtattc aggtgcagat ctattctttt tcccttctta
     8641 tgaagagaca gaagggatcg ttgttttaga agctttagca agcaaacaaa acgtacttgt
     8701 tcgagatatt ccagtgtata atggctggtt agaagatagc aagaactgct atatggggaa
     8761 gacaaacgaa gaatttatcc gactgatcca acagattacc aatcaagaac taccgagtac
     8821 gacacaagcc ggttatcaaa cagcaaaaga gcgaagtatc aataaaatcg gtgaagaact
     8881 taaaaatgta tacgaaagtg tactgaatga aaaagtctct agcaaattaa aaaaagtaaa
     8941 tcaggtcaag gactagtttg gaggcgaaaa acatgaaaat cggctttttt accgatacct
     9001 actttccgca agtcagcgga gttgctacat cgatcaaaac attaaaacaa gaattagaaa
     9061 gacatgggca tgaagtctat atttttacaa cgaccgatcc taatgcggat aagttggaaa
     9121 aagatgtgat tcgaatgcca agtgtaccat ttatttcttt caaggatcga agagttgttg
     9181 ttagaggcat gtggtatgct tatttggttg ccaaagaact agaactagat cttattcata
     9241 cccatacaga attcggagca gggatacttg ggaagatggt agctaagaag ttgaaaatac
     9301 ctctgatcca tacttaccat acgatgtatg aagactacct tcattatatc ggtaaaggaa
     9361 aagtgatcag gcaatcccat gtaaaatact tgtctagatt atttgcaaat catacgacag
     9421 gtgtagtttg tccaagtgaa cgtgttattg atacattacg tagctatggt gtaatagcac
     9481 cgcttcgcgt gattcctact ggtatcgaca ttgaaaaatt caaacgttct gatatcactc
     9541 agcaggatat cagcaaatta agggagtcac taggtcttca ggaaaaccat ttgatgattc
     9601 tttctttaag caggatttct tatgaaaaaa atatccaagc cttgattaat gggtttcctc
     9661 aggtcatctc cgcttatcct aacgccagat tagttatcgt aggaaaagga ccttatgtag
     9721 aagagttaga ggaattgatc tcagaactgc agttagaaga atttgtgcag ttcacaggag
     9781 aagtggacaa caatgatgta gcactttatt ataaagcggc agattatttt gtcagtgctt
     9841 ctacttcaga aacacaaggg cttacttata cagaagcgat ggcagccgga acaccttgtg
     9901 tcgtggaagg aaatgcttat ttgaaccgat tgttcgatca tccttcatta gggaaaactt
     9961 ttgaaaccga cgaagacttt gcaccagcac ttattgatta tatttccagt gaagttccta
    10021 aagatccaaa agttttagaa gctaaacttt atgatatttc atctacacat ttcggcgaag
    10081 ctatgatcga attttatcaa gacatggttg tttatcatga agaagagatg cgaagaaaag
    10141 aagaagaaga ctcgattgaa cgaatcaaaa taaaattaaa ttcatttaaa agataaaatt
    10201 caaacatttt ttatgaaaaa atccttgttt ctagcgtata ctagcataga agcaaggatt
    10261 tttgattttt aggaggtcga aaaaatgaaa cttggtttta tcggaacagg cgtcatggga
    10321 agtgctgtcg cacgccatct actagaagca ggacacgaag tagctgtcta taatcggaca
    10381 aaagcaaaag ctgatccttt agtaacagaa ggagctatct gggcagatac accaaaagcg
    10441 gtagcagaac aaagtaatat tctctttaca atggtaggtt accccaaaga tgtagaagaa
    10501 atctactatg gacagtcggg gattttttct gcagatatct caggacatat tttagttgac
    10561 ttgactacaa gtacaccaag cctggcagaa aaaattgcaa aaacagcaaa agaaaaaggc
    10621 gcagatgcgt tagatgcacc ggtatccggt ggtgacttag gagcaaagaa tggtacactg
    10681 acgatcatgg tcggtggaga agaagccgtt tatgatcaag tactgccttt attcaaagaa
    10741 tttggtacga catttacatt gcatggttct gctgggaaag gtcagcatac aaaaatggct
    10801 aatcagatca tgattgcagg tacgatgaca ggaatgacgg aaatgcttgt ctatgcacaa
    10861 aaaaacggac ttgatttaaa gaaagtgatt gagacgcttt caggtggaag tgcagctaac
    10921 tggtctttaa gcaattattc tcctcgtatt ttaaaagaag attacacacc tggttttttt
    10981 gtcaaacatt ttatcaaaga tttaaagatt gcattagaag aagcagaaaa aatggacctt
    11041 gtattgccag caactaccca agcattgaaa ctatatgaag agttggcaga taaaggtttt
    11101 gagaatgacg gaacccaagc tttgatcaaa ctttggtggc ctgaaggaaa aataccagaa
    11161 aaaaaatcat aaaaaacgag tttttttaga gaaaatcaat gcaagtaggc tatgcttttg
    11221 atacaatata acagaaataa gaaaaggagg acctccgatg acacattcac aattagtcgc
    11281 aatcataaaa cgactggagg ctatggtcga gtcagcagat aatgaactgc aagttcgccg
    11341 ttttgaaaaa gaaggcgtag agaagtgtac agtaacttat gataaagcga ccgatacatt
    11401 tgaattaact gaaacagata ctcatcagca atacgaattt gataatatcg atatcgtagc
    11461 gatggaaatc tatgacttaa tccaataaat tttagaataa ttgacatttg aagttgaaga
    11521 tgcctaaaag aagccgagaa gcaactaatt tagttgcttt tcagcttctt ttttgtttaa
    11581 cagttcaatt tcagactgtt ctttttctaa ttttttgcgg taactgatca agatttgtac
    11641 aggatcgatt tcttcttttg acaattgacg tttcaaagat aaatgagctt ctttcttttc
    11701 ataatagaag tcaggagaaa cgtcagcaga ggcaatcatt gtttctgcaa gttgaccata
    11761 acaaacgagg tagtgctttt tctgactctc atctgctgct aatggcatgc tgaaggtgag
    11821 cgatggtgtt cttaaaaaat gcttttgttc ctggatccgt ctaataaaat aagtaactaa
    11881 ttcgatatct ttttgaagca taccggcttt ttcgaaattc aactgataga tgaaatgttg
    11941 ttgtcttttt ttcaagtaag ttagtagctt tttattttgg ccttgaaaca gcccttggag
    12001 ttcctttttt ttctgttcaa aagtcatttc ctgtaggtca ggaagctggg ctcgcaaagc
    12061 taacaaacta atttcagaca accaaggcat ttgataagtt tcttccatta tacgcaaaat
    12121 agacggcatt tttttataga gacgaaaagg cccgtatgtt cgagccgtag gagtatctgt
    12181 taatacaaat cccttattgg taatatgtac ataattataa ttactgaaat aattcatttg
    12241 tcggttataa aaaggacggt aatgttggat caattgacac tcgagcaata aagcatctaa
    12301 ttccgtgtct actacgacga aatcaaaatc tgttatatga tgaatcatac gtaatacttt
    12361 ttttgaatgc tgtttattct gatgaaaata gctgcttact ctatttttga gccgttttgc
    12421 tttaccaaca tagatgacct taccatcacg gtctttcata agatagacac ccggcagcat
    12481 ggggagagtt ttcatttttt cttttaacat aggatctcc
//
LOCUS       DAXWLF010000074        12193 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXWLF010000074 DAXWLF010000000
VERSION     DAXWLF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.074"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(155..319)
                     /locus_tag="ACGE4U_001547"
     CDS             complement(155..319)
                     /locus_tag="ACGE4U_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393718.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            614..1942
                     /locus_tag="ACGE4U_001548"
     CDS             614..1942
                     /locus_tag="ACGE4U_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFF8393719.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2144..2977
                     /locus_tag="ACGE4U_001549"
     CDS             2144..2977
                     /locus_tag="ACGE4U_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFF8393720.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2993..3730
                     /locus_tag="ACGE4U_001550"
     CDS             2993..3730
                     /locus_tag="ACGE4U_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFF8393721.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3885..4853)
                     /locus_tag="ACGE4U_001551"
     CDS             complement(3885..4853)
                     /locus_tag="ACGE4U_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFF8393722.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5085..5918
                     /locus_tag="ACGE4U_001552"
     CDS             5085..5918
                     /locus_tag="ACGE4U_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFF8393723.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5952..6791
                     /locus_tag="ACGE4U_001553"
     CDS             5952..6791
                     /locus_tag="ACGE4U_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8393724.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6817..7377
                     /locus_tag="ACGE4U_001554"
     CDS             6817..7377
                     /locus_tag="ACGE4U_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8393725.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7576..9297
                     /locus_tag="ACGE4U_001555"
     CDS             7576..9297
                     /locus_tag="ACGE4U_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFF8393726.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9462..10604
                     /locus_tag="ACGE4U_001556"
     CDS             9462..10604
                     /locus_tag="ACGE4U_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFF8393727.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10620..11831
                     /gene="thiI"
                     /locus_tag="ACGE4U_001557"
     CDS             10620..11831
                     /gene="thiI"
                     /locus_tag="ACGE4U_001557"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFF8393728.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4154 a         2047 c         2533 g         3459 t
ORIGIN      
        1 gagtcctact gccggagtaa ataaagctta tgcaaagaaa aagaaatcca atcacttggg
       61 tttctttttc ttttattatt atgatgaatc aagctttctg tttttgtcac gtatgttgta
      121 atctttacga aagagtagag ttggctactc ataatcaacc atacttccca tcatcgctaa
      181 atacgaaata aatcagcatt cctacaccaa tcaaagcaca agctgcacca agaaaaggga
      241 tgacattccc aaagttatct gtattcataa gtatgccacc acccacaaac aaaaagccgc
      301 cagcaatcag gtttttcatt ttattcatct cctttcttac gttatagtat ttccttcttt
      361 caatgagtat tttgaaagct atcttattgt atgatgttct tctaggcctc ttctttatta
      421 tacaggccat tttcgttatc tcatagaaat attccttttt atctgaaaac tattgtatac
      481 cgtatgacat ttttcttcat gaagttgtaa gtatattctc attcaaatga aaatttcatc
      541 atagaagaga ttgtatttct aaattcttaa gagtaaaata cgaagtataa cttggatttg
      601 gaaggatgat tggatgaaag ctattacgcc tgatatgaca acaaaattta acaaacagtt
      661 tcaagcaaat atcaaacagc aggcattgca gcgggcagtt gtgaagaacg gaattactgc
      721 ttctgctgaa aatgtttcag cacatgtgga gaatacaccg gtattttcta tagatttggc
      781 tacggggaaa gtggctaatc aaatgcaatc ggggcgttgc tggatgtttg ctgcactgaa
      841 cacattccgt cataagatgc tgaatcaatt ccatttaaaa gagtttgaat tatcacaaaa
      901 ttacactttt ttctgggata aatatgaaaa ggcaaattat ttctatgaaa acatcattgc
      961 aacaggaaaa gaggcaatcg atagccggaa agtagctttc ttactcgcta ctcctcaaca
     1021 agatggcggc caatgggata tgatcgtttc attattccaa aaatatggcg ttgtgcctaa
     1081 atcggtgatg ccggaaagtg cgaatagttc aaattctcga gatttgaata actacttgaa
     1141 caaattactg cgtaaagatg cagtagtgtt acggaaaatg gttgctcaag gagaaacatt
     1201 agacgctatt gaagaaaaga aagaagaaat gctagaaagc atttataact tcttagctat
     1261 ctcattagga acacctccaa aagaattcga ttttgagtac cgcgatgaag aaaagaacta
     1321 tcatcttgac cgaggactga caccacaaat tttttatgat aaatacatcg gtgtaaaatt
     1381 agatgattat gttagcgtga tcaatgcacc aacaaaagac aaaccattca atcgttctta
     1441 tacagtagag atgctaggaa atgtcgttgg cgggaaagaa gtcaaatatc tgaatgttga
     1501 tatggaaact ttcaaaaaat tagcgattgc tcagcttgaa gaaggagaat ctgtttggtt
     1561 tggttgtgat gtaggtcaat cttctactag aactagtggc atcatggcct tagatgctta
     1621 ttcaatggat gatctatttg atacggattt tacaatgact aaagctgaac gtttagattt
     1681 tggagaaagt ctgatgaccc atgcgatggt attgaccggt gttgacttag tagacgggca
     1741 atcaacaaaa tggaaagtcg aaaatagctg gggcgaaaaa gttggaaaga atggcttctt
     1801 tgttatgagt gatgcctgga tggacgaata tacgtaccaa atcgttgtaa gaaaagattt
     1861 attgacgaaa gagcaattaa atgcgttcaa tgaagaacca attgttctat ctccgtggga
     1921 tccaatgggt gctttagcat agacgagtat ttcaatgaag agtatgtagt aaagataaaa
     1981 agagctggct taagccagct ctttttatct ttaaagtcca ttccctgttt tttatggtcc
     2041 ctgtgctata attctaaata aaggataagg attggaaata atccttttat cattaaaaat
     2101 atgatgacga agtagaataa aattgggagt aggggggaaa gtcatggata tccaaactgt
     2161 tttatctgat ctgaaattaa atggtaaagt gattccagtt gttggtggcg atgttaatca
     2221 gacctatcgt ataaaaaccg aacatcgtgc ttatttttta aaaatccatc cgaatgtaaa
     2281 aaaaggattt tttgaagcag aagtagacgg attaaaagaa ctgtcagcat tcgtacgtgt
     2341 accggatacg tatatgctag gtgaaacaag tgaaggcgct tatttgttaa tggaatggat
     2401 cgagccggga aaaggggatc aacgagatct tgctgcagct ttagctaatc tccatcagca
     2461 aacagcgcca caatttggtt ttcgtaaaga taactatttg ggtacacttg ttcagaaaaa
     2521 ttcttttgaa gaagattggt ggactttttt ctttaaagat cggcttgaat cccagatttc
     2581 tttggcagaa gaaacgaatc gatggaatgt acagcgtcaa gaaaagtatc tgcgatttaa
     2641 agaacgtgta ttgaaatcgg ttgaaccaaa aaagatcact ccacgcctct tgcacggaga
     2701 cctgtggagt ggtaatgtgt tctttgacca gcaagggcat ccagtttttg tcgacccagc
     2761 tgtttcttat gggaatcggg aacaagatat tgcaatgagc cagttattcg gtggtttccg
     2821 accggaattt ttagatgctt atcaaactat atttccatta gaaaagggat ggaaagaccg
     2881 actgccgatt taccagttat attatctttt ggcacatttg aatatgtttg gagaaagtta
     2941 cggttcccaa gtagatcaat tactagaaaa tttttaagag gagggcgtgt ttttggcaaa
     3001 aaaatatacc aaaaagcatg aagtttcata ttatgagtgt gacatcaacc aaacgatgac
     3061 atttccttcg atgttaggag tagttatcaa aacgtctgaa gatcaaagcg accagttggg
     3121 aagaggcagt gaattcgtca atagctttgg acttacttgg gtcatcacga attattccat
     3181 ggagattaat cgtctcccta gagtcggtga agtaatctca gtaacgactc aagccatgga
     3241 gtacaataag tttttctgtt atcggaactt ctggatccat gatgaagatg ggaaagaatt
     3301 agtcaaaata gaatctgttt tcgtattgat ggattttgtg aatcgaaaaa tgagcagtgt
     3361 taatgaagag atcattgctc cttttgagag tgagaagatc aagaaaatca agcgtcagga
     3421 aaaaattgaa aagattgatt cgggcttcat gttgccatat cgtgttcgtt tttatgatat
     3481 cgatagtaat cagcatgtta ataatgcgat gtattttaat tggatcattg acgtattagg
     3541 atatgacttt ttgactacgc atgttccaga aaaagtaatt attcgatttg ataaagaagt
     3601 agagtacggg aatgagatcg aaagccattt tgaacaaatc cctatcgaaa atggtgtcaa
     3661 aacaaaacat gaaatccgaa tgcaagaaca gctgtattgt gaagcaaata ttatatggaa
     3721 aaataaataa aaaagaggtt gtgacagaag ggggcaactt caagaaataa gaagaaattt
     3781 acgaaaattg tttctcaaat ttttgtaaat tttcgactta gttttcgaag aagctatttt
     3841 tgtcacagcc tctcttttta agtttagcta ttagataatg atgtttagtt ttggataaac
     3901 gaaatcaatt cttcacccga caaaccatag tttacagcaa tcaatagttt taaacgtgct
     3961 ttttgactat taatactgtt acagaaaata atccccattt tttctagttc ttttcccccg
     4021 ccttcataat cataaacagg ctctgcgatt ccattgaaac aacgagaaac aagtactaca
     4081 gggatttttt ggacaagaag cttttccaaa gcagctaagg cttgtggagg gagattcccc
     4141 gcacctaaag cttcgattac caaaccgtct actttcgtat gggcgatggc ttccaataag
     4201 tccccttgca tcccagcata tgctttaacg atcgggatag tcccatctac agcggaaatg
     4261 tccagtcgct ttgtttctaa aagttcctgc aagaataaaa ttcgattctt agttactaag
     4321 ccgatgggac ccaatgtagg tgttcggaaa gtagcaacat ttgtggtatg ggtctttgtc
     4381 acgtagcgtg cagaatggat ctcatcgttc atgaccacca aaaccccctt atctagcgct
     4441 tcttcacaac ttgcgacacg aatcgcactt tcaaaattat aaaggccgtc acttccaagt
     4501 tcattacttg aacgcattgc cccagtcaaa acaattggta atttgttgcc aatcgttgta
     4561 tcaagaaaaa aagcagtttc ttctaatgta tctgtaccgt gtgtgatcac cactccggat
     4621 atacctgatg cataagcgga caagatacgt tcttttaata ccaacatgtg agaaggcatg
     4681 atgtgtggtg agggaagatt gaagatatct tcaactaata attctacatg cttggggatt
     4741 gtgatttctg catctaataa tgggtttgcc acatcaggac taacaccgcc cgtgagcatg
     4801 ttttctttca tggcgatcgt tccgccggtg tgtagtacaa gtattttttt cattttacag
     4861 acttccttac gaataatttt gcatagccgt taaatcttat tgtatactat ataaaagagt
     4921 tttgctatca gtgaagaata acgaatagcc taacttatca taaaaaagct gaccttatta
     4981 aaatggaaaa gactcaacct tgacctccta atacaatgga gtatctggtt gagtttcttt
     5041 tacaagaaag cagccaagga aatcaatcac gaaggtggcc ggaaatgatc agaatcggat
     5101 taacatcgtt taaagaacat gacaaattaa ctggtaaaaa gaataacact ctttatgaat
     5161 atgcagggtt tctaccctta gtggaattag atactgccta ttatggaatt ccccgaaagt
     5221 cctcagttga gaattggata agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct
     5281 atcaaggaat cagcggtcaa ggagactgga agccttatta tcagaccgaa gaagagatga
     5341 ttgcgcattt tttagatgtt atgtcgccaa tggtagatgc tggaaagatg ttttgctttt
     5401 tagtccaatt cccctcatca ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac
     5461 ttagaaagtg gtttgagggt tatcctgttt ctatagaatt gcgggattat tcttggtaca
     5521 gcgatgaatt tcttgaacgt acaagaaact ttatgtctag agccgctttt tctttagcca
     5581 tcatcgatga gccacagttg ctgaatacaa cgattccttt tgatccattt gtcaccaatc
     5641 cgaactttgc tctttttcga ttccatggta ggaatgcgca aggatggcaa gcaaatgata
     5701 aagagtggcg taaaaaaaga acgttatatc gttattcaga aaaagaactg caagaactag
     5761 cacatgaagt caaagtagtt gattctaaga caaaagaggt aggtgtgatt tttaataata
     5821 attctggtgg tgatgcggct gaaaatgcat tagcactgca aaaaatatta aatatcaagc
     5881 cagatggact caatccaacc caaatggatc tgttttaagg gcttatttta ttcaagaaag
     5941 gggcgaagtc tatgatcaaa gctatttttt ttgatattga cggaacattg gtcaccaatc
     6001 gttcaaaagc gttggaatca acaaaacaag caattgaata tgctcggaaa aacggcattt
     6061 tatgcggtgt agcaacgggg cgcagcccag taaagatcaa agaaatcatt gatgaattgg
     6121 aattagatat gtatgtcgtg tataatggtc aactggtttt tactgcagat cgcacgatta
     6181 tcgaccaccc ttttgaacaa aaagtattgg aacatattgt tgaatttgca gatgagaacc
     6241 atcgacaaat cgtttttggt gcgagaaatc ggttagatgg atcaacgacg atgctattag
     6301 gacaatcgat ttttatcaag cgactagtca gctttttacc gcgtaaattc ccggtacgtc
     6361 tgatgaaaaa aatcctccag gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt
     6421 tagagatatt aaaagaacca atttatcaat gtatattgct cagtccggag tcagaacaga
     6481 aaaagttaga acatcgcttt cccgagtgta cgtttcagcg ttcaaatacg tatactgtag
     6541 atatcatccc aaaaggtggt tcaaaattac taggcatcca agctttcgca aatgctgtcg
     6601 aaattgaaat ggaagaaatt atggcattcg gcgatcatta caatgatatc gagatgctga
     6661 aaggtgtagg aattggagtg gccatgggga atgctcagat cgaagtaaaa catgctgcag
     6721 actttgtgac tcaatcaaat gaagaagatg ggatatacga aggactgaaa cacttcggtc
     6781 tgatttatta gtcaatgaaa gaaggaaaaa atcagtatgg aagcagagac accttttcaa
     6841 aaagtagaaa cattccacaa aacttttgat ccaagaagac cagagatacc cacggctttt
     6901 acagaaaatg aagctcatac acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt
     6961 gcagctactt cacctaataa agaacaactg catgaagctg ttatgctgct gcacgaagca
     7021 ttagataaag cagaagaaaa aatacttggt aaaacacagt ggggaagtga tccgctggtt
     7081 gaacaagtag atgcactgac agatatcctt tactttactt atggttcttt ttcattacta
     7141 ggaatagtgc ctgatcgaat attcgaaatc gtccatcagg caaatatggg gaaattattt
     7201 ccagatggaa aacctcatta tgaccctgta acaaataaag tgttgaaacc tgaaaactgg
     7261 cagatggaat atgctccaga acctaaaatc gcggcagaaa tccaacgtca gattcaagaa
     7321 gcgattgcta agaagaaatc cgctgatata ggcatgtgca caaatgaaaa agagtgatca
     7381 gagtttcact atttttgtta tctttatacg atggaaaaac aaaagatttt cgtttaagtt
     7441 agtgaatgca tgaaaatagt atgctataat ctagaataga tgtcttttgc acgagagacg
     7501 tattatttgg atttatgcca agcagaacgc catctgttgc ataaaagatg catactgaaa
     7561 acggaggaac cataaatggg aaataatgtt atcatcggaa tcgtcattgc aatcattata
     7621 gtcgccattg tcgtcttttt ggttagttac ttgatgaaga aaaaaaacca agatcgattg
     7681 gatgccttag aaaaacgcaa ggaagaatta tttgacttgc cagtgattga agaagtcgat
     7741 gatgttaaaa aaatgcatct ggttgggcaa agccaaaata cctttcgtga atggagtcaa
     7801 aaatggaatg acatatctac ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg
     7861 gaaaacctca atgaaagttt tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa
     7921 gctacaatgg accaaatgga gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg
     7981 cgggaaacgg aagaacgcaa ctcactagct gttcagcagg cacttgacgt ctatgaagaa
     8041 ctgaaagaaa aagtcagaga agacgagcat gaatacggac cagctctttt tgaattgcaa
     8101 aaacagatca aagtgatcga aaaagaattt acgcagtttg tgactttaaa cacttctgga
     8161 gaccctgttg aagcccgcga ggttttggat aaagcagaaa aacatacgta tgaagtggaa
     8221 gctctgatga aaaaaattcc accgctgtat gaggagctcc ataccacttt ccctgaacaa
     8281 cttgaagaaa tcagtgatac ttacgagaaa ttgatggaag aacaatatgt attcccagaa
     8341 gaagatcttg ctgaagatat cgcaaaagta agtcgaagaa ttgaaaattc attagccaat
     8401 cttgaaaaaa cagaagttga aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt
     8461 gattcgttat atgatatctt agaacgggaa atggaagctc agagatatgt aaaaactaac
     8521 caatcaacga ttgcagagta tatcaaacat acaacaaaga ataaccgtca attattgatt
     8581 gagctggatc acacatctca aagctataca ttgaaccaca atgaaattgg ccgtgtacga
     8641 ggtttccaaa ccgaagtgga agaaatggaa cgtcaaaatg aacagatgat tccacagatc
     8701 cgtcaacacg aaattcctta ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc
     8761 ttagaagata tcgaaaagca acaagtagag atcgacgact cattacatga attgagaaaa
     8821 ggcgaaaaag aagcgcaaga aaagatcgat acattcgaat tcaaattgcg cagtttgaaa
     8881 cgttttgtag aaaaacaacg tttgcctggc ttgccgaatg attacctaga attctttttt
     8941 gttgctactg accgtattga agaactaagt gttgtcttga ataaaattcg agtaaacatg
     9001 gaagaggtca atcgtttagt tgctctatgt gaagaagatc tagaattatt agataagaag
     9061 acacatgatt tagtagatgc agcagcatta acggagcaaa tgctccaata tgcaaatcgt
     9121 tatcgtcata cacacgaaga agtgagagaa gcaattgata aaagctacta tttattcaat
     9181 aaagaatatc actatcaaga agcattagac gaaatcggta cagcattaga gcgagtagaa
     9241 cctggtgctt tcaaacgcat cgaagatttt tattttaatc atccagactt agtataaagc
     9301 aaaaaagtta gtaaaccagc aagagcaaga cggattattg tcttgttctt gtcttttttt
     9361 gtatatttga aaattgaaac aagtgaaaat ttcacctgct cttgaaagca ccttggacaa
     9421 ccgttataat agaaaagaat ttcaaaaaag aggggtttct tatgatatat tttgataaca
     9481 gtgcaacaac cccaatcttt ccgcaaagct tagatgcata tgtaaaaacc agtcagcgga
     9541 ttattgggaa tccatcaagc ttgcatgatt taggcaatca agctaaccgg ttgttgcagc
     9601 aagcaagaaa gcagatcgct gatttgatcc atgtagaacc agaagaaatc tattttactt
     9661 caggcggaac agaaggagat aactgggtat taaaaggaac gatgatcgaa aaaagagaat
     9721 atgggaatca tatgatcatt tcaggagtcg aacatccggc cgtcagcgag acagcagaac
     9781 aattaaaaga attaggaatc gaagtaagta tcgccccagt ggataaacgc ggatttgtac
     9841 gtgtcgacga attgaaagaa ttaataagaa aagagacagt attagtctct gttatggctg
     9901 tgaataatga agtaggatcg attcagccca tccaggaaat cagtgatctt ttggatgatt
     9961 ttcctaaaat tcatttccat gtagatgctg ttcaggcgat tggaaaagta ccattctcgc
    10021 aatggttgac agaaagagtt gattttgcaa cattttcagc tcataaattc catggcccac
    10081 ggggaactgg tttcatttat tggaaaaagg gtcgtcgttt agcaccatta ctgacaggtg
    10141 gagggcaaga aagaaatcag cgaagtggga cagaaaatgt tccaggtatt gtggctatgg
    10201 ttaaagcttt acgtttatca ttagagataa aggaacaaaa acctgagcag acacaacgct
    10261 taaaagtagc tctaatcaat gcgttgaaga cgtatgagaa agtgacgatt ttttcagaag
    10321 gaactgacta tgcaccgcat attttatgtt ttgctctaaa aggaattcgc ggcgaagttc
    10381 tcgtccatgc cttagaagaa aaacaaatat ttgtttctac tactagtgcc tgctcctcac
    10441 gtaaacatat ggcaagcagt acattgcacg caatgcatgt accaaatgat ctggctacat
    10501 ctgctgttcg aatcagctta gatgaaaaca attcgcttgc agaagtcgag caatttatga
    10561 ttattttcaa gcagctttat aagaaatttt ctgcaatcaa ttaaaagagg agtgttcttg
    10621 tgaaatacag tgaaattatg gtccgttatg gggaactttc gacaaaagga aagaatcgaa
    10681 aaagctttat catgcaacta gcacaaaatg tcagacaagc tttgaaagac tttccagaaa
    10741 tcaaaatcca tgctgatcgg gaccggatgc atcttttatt gaatggcgca gacgggcagc
    10801 tagttattcc aaaactagca aaaatatttg ggatccaaaa tttctctcca agtattcgtg
    10861 tagaaaaaga tgtcgaagtt ttgaaacaag ctgttcaaga catcatgaaa gaaatttata
    10921 ctgggaaaga aacatttaaa attatcgcta aaagaagcga tcaccaattt gaattgacaa
    10981 gtaatgaatt gaatcaaaca ttaggtaatg ctgtttttga tattttcccg catatccaag
    11041 ttcaaatgcg tcaaccagat attccattac gtgtagagat tcggcgtgat ggcgcttatc
    11101 tatcgtatga aacgatcaag ggagctggag gattgcccgt aggaacaagc ggaaaaggaa
    11161 tgcttatgct ttctgggggg attgattctc ctgtagcagg gtactttgcc atgaagcggg
    11221 gagtggaaat tgaagctgtt cattttgcta gtccgccgta tacaagtgaa caagcactgc
    11281 aaaaagcaaa agatctaaca gccaaactag caccttatgt gggaacgatc cattttatcg
    11341 aagtgccatt taccgagatt caagaagaaa tcaaaaagtc agtgccacaa ggatattgga
    11401 tgacaattac tcgtcgaatg atgttgcgtt tgaccgatga aattcgccgt attcgtcatg
    11461 gtttagtcat tttgaatggt gaatcattag gtcaagtggc ttcacaaact ttgcaaagta
    11521 tggtggctat caatgaagta acgaatacgc caatcattcg gcctgtagcg acaatggata
    11581 aacttgaaat cattgaagta gcagaacaga tcgatacatt taatctttct atccagcctt
    11641 ttgaagattg ctgtacgatt tttgctcctc cacagccaaa aacacgccct caactggaaa
    11701 aggtccatca atatgaagag cgtttagcaa tagatgaaat gatccaacga gcactagctg
    11761 gcttgaagat ggaagagata caagcagaat cgtctaatga aaaaactgaa gaattttcag
    11821 aattgctata agaggttatg aaaatagtgt tttgaccaga gtattagaga aacaaaacga
    11881 aaaaatagct ccatgtattt taccgttttt gaactaatgt cgaagattag ctgattgaat
    11941 accatttatc aagaagttat ggaaaagccg gttctttttt actaactgaa aaataaaatt
    12001 ttatttagag atcaagctac agttgataaa tgatataaaa aagagaattt cccatgaaat
    12061 agcgaggttg tgacagaagt gggcagcttc aagaaataag aaggaattta cgaaaattgc
    12121 ttttcaaatt tttgtgaaat ttcggcttat ttttcgaaga agttacttct ggaacaccgt
    12181 gtatcagagg ttg
//
LOCUS       DAXWLF010000075        12136 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXWLF010000075 DAXWLF010000000
VERSION     DAXWLF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.075"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..610
                     /locus_tag="ACGE4U_001558"
     CDS             <1..610
                     /locus_tag="ACGE4U_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8393729.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACGE4U_001559"
     CDS             723..1001
                     /locus_tag="ACGE4U_001559"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8393730.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACGE4U_001560"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACGE4U_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFF8393731.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACGE4U_001561"
     CDS             1458..2213
                     /locus_tag="ACGE4U_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8393732.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACGE4U_001562"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACGE4U_001562"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFF8393733.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACGE4U_001563"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACGE4U_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFF8393734.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACGE4U_001564"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACGE4U_001564"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFF8393735.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACGE4U_001565"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACGE4U_001565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFF8393736.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACGE4U_001566"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACGE4U_001566"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFF8393737.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACGE4U_001567"
     CDS             6950..7492
                     /locus_tag="ACGE4U_001567"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFF8393738.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACGE4U_001568"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACGE4U_001568"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFF8393739.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACGE4U_001569"
     CDS             9085..9987
                     /locus_tag="ACGE4U_001569"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFF8393740.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACGE4U_001570"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACGE4U_001570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFF8393741.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACGE4U_001571"
     CDS             11430..11852
                     /locus_tag="ACGE4U_001571"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFF8393742.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3269 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttattt
//
LOCUS       DAXWLF010000076        12124 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXWLF010000076 DAXWLF010000000
VERSION     DAXWLF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.076"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(80..637)
                     /gene="frr"
                     /locus_tag="ACGE4U_001572"
     CDS             complement(80..637)
                     /gene="frr"
                     /locus_tag="ACGE4U_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFF8393743.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(640..1362)
                     /gene="pyrH"
                     /locus_tag="ACGE4U_001573"
     CDS             complement(640..1362)
                     /gene="pyrH"
                     /locus_tag="ACGE4U_001573"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFF8393744.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1498..2379)
                     /gene="tsf"
                     /locus_tag="ACGE4U_001574"
     CDS             complement(1498..2379)
                     /gene="tsf"
                     /locus_tag="ACGE4U_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFF8393745.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2478..3260)
                     /gene="rpsB"
                     /locus_tag="ACGE4U_001575"
     CDS             complement(2478..3260)
                     /gene="rpsB"
                     /locus_tag="ACGE4U_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFF8393746.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3619..4098
                     /locus_tag="ACGE4U_001576"
     CDS             3619..4098
                     /locus_tag="ACGE4U_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8393747.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4315..5406
                     /gene="phnW"
                     /locus_tag="ACGE4U_001577"
                     /pseudo
     CDS             4315..5406
                     /gene="phnW"
                     /locus_tag="ACGE4U_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            5399..6184
                     /gene="phnX"
                     /locus_tag="ACGE4U_001578"
     CDS             5399..6184
                     /gene="phnX"
                     /locus_tag="ACGE4U_001578"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFF8393748.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6530..8221)
                     /gene="argS"
                     /locus_tag="ACGE4U_001579"
     CDS             complement(6530..8221)
                     /gene="argS"
                     /locus_tag="ACGE4U_001579"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFF8393749.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="ACGE4U_001580"
     CDS             complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="ACGE4U_001580"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFF8393750.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9705..10724)
                     /gene="argF"
                     /locus_tag="ACGE4U_001581"
     CDS             complement(9705..10724)
                     /gene="argF"
                     /locus_tag="ACGE4U_001581"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFF8393751.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="ACGE4U_001582"
     CDS             complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="ACGE4U_001582"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFF8393752.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3321 a         2526 c         2185 g         4092 t
ORIGIN      
        1 tttttaattt ttaaataaaa gaaatataaa taaaaacaga ggaagcggaa tgatccgccc
       61 ctctgttttt atgattttat taaacttcca ataattcttt ttctttatca gcagcgatct
      121 tatctacttc tttaatgctg ttatctgtca atgtttgtac gtctttttct aatccgcgca
      181 aatcatcttc ggtaatatca ccattttttt gttgtttttt atattcatcg atcgcatcac
      241 gacggatgtt acgaatcgcg atttttgcat tttctgcttc ttttttcaca tctttagcta
      301 attcttttct gcgttcttct gtcagttgcg ggataactaa gcgaatgaca tttccatcat
      361 tggttggact gattccgata tcacttgcca taatagcttt ttcaacatct tgaagaatgc
      421 tcttgtcaaa aggtgtgatc attaaaacac gtgcttctgg aatttggatt gatgccattt
      481 ggttcaatgg tgttggcgca ccgtagtagt tcacagtaat acggtccaaa aggctagcgt
      541 tcgcacggcc tgcgcggatt tgcccaagtt cacgttgaag attttgtgct gcttttttca
      601 ttttttcttt tgcttctgtc ataattgcat ctgccatttt tatttccccc ttacagttgt
      661 tccgatattt tcgcctaaga ttgcacggcg aatatttccg tgttcattta aattgaagac
      721 aagcagtgga atatcattat ccatgcttag tgaacttgca gttgaatcca tgacttgtaa
      781 tcctttggca ataacttcca gatgagtcag ttcttcaaat tttactgcat ttgcatctac
      841 tttcggatca gcagaataaa caccgtctac attgttttta gccatcagga taacatctgc
      901 gccgatttct gccgctcgta atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt
      961 accgcctgca aagataacga tccgtccttt ttctaaatga cgttctgctt tacggcgaat
     1021 atatggctct gcgatttgac gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac
     1081 gttttccaac gtatcctgta aagctaaggc attcataact gtagctaaca tacccatgta
     1141 atcagcttga gcacgttcca tgcccatttg tgctccgatt tgtccgcgcc agatatttcc
     1201 gccacccaca acgattgcca tttcaacgcc cagctcgtga acttctttga tttcttggat
     1261 gatttctttg attactggtg gtttgattcc aaatcctgca tccccagcta aagcttctcc
     1321 actcaatttt aagactacac gctgatattt cggtgtgacc atgttcattc ctccaatatt
     1381 ttctattgcc attttaccat acttagatga tttcgacact ctaagactgg tttttcttgt
     1441 tttgcttaaa aaaagggagc gcacaaaccg tgcgttcccg tctgttattc aacagaatta
     1501 ttttttcact tggctcataa cttcttctac aaagttatct tcgcgttttt cgataccttc
     1561 gccaacttcg aaacgtacga atgttttaac agttgcgcct tttgatgcaa cgtatttttc
     1621 aacagtcata tcaggatctt taacgaatgg ttggtccacc aaagcgattt ctgctttgaa
     1681 tttcttcaag cgtccttcta ccattttttc aacgatattc gctggtttgc cttcgttcaa
     1741 tgcttgttct gtaagaacag ttttttcatg ttctaattca gcttctggaa tttgtgattc
     1801 gtttacataa cgaggattga tagctgctac gtgcatagct acgtctcttg caactgtttc
     1861 gtcagttgtt ccatctagaa cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc
     1921 accaaatgca gcattgtcgt ctttttctac aacttcaaag cgacggaagc tgattttttc
     1981 tccgataact tgagttgctt cgatcaagtc agattcaatt gtacctttat ctgttttgat
     2041 tttcattgca gcttccatgt ctgctggttt gttttcagca acaagttctg caatttcttt
     2101 tactaaatct tggaacattt cgtttttaga aacaaagtct gtttctgagt ttacttcaac
     2161 gattgctgca gtgttacctt tcacagcaac tgaagccaat ccttcagctg caatgcggtc
     2221 atttttcttc gcagcctttg ccattccttt ttcacgtaaa agatcaactg ctttttccat
     2281 gtcgccttct acttcaacta atgctttttt cgcatccatc attcctacgc cagtcatttc
     2341 gcgtagttct tttaccattt tagctgtaac gtctgccatg tttaactcct cctgtgtgtt
     2401 tttcttttta aaaaaagctg cttcaaagga gagtgaggct tgtcgcccag cctttgaaac
     2461 agctccatca ttcaaaatta ttctgctgaa gaattgtcgc cttcaacaac atctacgatt
     2521 tcttcgattg atgttgctgt gttttcttct gtgaaatctt cttcaactac ttgatcttca
     2581 ccttggtttc cttcgatgaa agcatcagcc atttttgaag taatcaattt aacggcacga
     2641 attgcatcgt cgtttgaagg gattactacg tcgatttcat ctggatcgca gtttgtatca
     2701 accatagcta cgattgggat atttaatttg tgtgcttctt gaacagcaat acgttcttta
     2761 cgtgggtcaa cgatgtacat tacatctggg attcttggca tatcagcgat accgcctaag
     2821 aatttttcaa gacgttcacg ttctttgttc aaaccagcaa cttctttttt aggtagaact
     2881 tcaaaagttc catcttcttc cattgcattg atttgtttca aacggctgat acgtttttgg
     2941 atagtatccc agtttgtcaa tgttccgcct aaccaacgat ggttaacgaa gtattgtcct
     3001 gcacgagttg cttcttcttt gattgcttct tgtgcttgtt tttttgtacc tacgaataat
     3061 gctacgccgc cttcttcagc aacgctcttc atgtaatcat aagctgcatc tactaattta
     3121 actgtttttt gcaagtcaat gatgtagatt ccgtttcttt ctgtgaagat atatttcttc
     3181 atttttggat tccagcgacg agtttggtga ccaaagtgta cgccggcttc tagtaattgt
     3241 ttcatagaaa ttactgccat gtttgtttcc tccgatttgg ttttattttt cctcttctta
     3301 tcttctacct tgccggcaaa ctttttcaag cacctaccga caatccaata agaatgtgga
     3361 tttgctgatg cataaagcac cctcagctat tatacagaca aaagatccgt atcgcaagag
     3421 caaatatact tttctgctca acattcaata gtttacctga aagataaaaa gaattcaaga
     3481 aaaacagtag atttctcttt gtttctttat tatccttttt ccataaaata attttaggta
     3541 taaaagacga atattattgg gcaatccttc ttttttttgt tatgatacat gtgaaatctt
     3601 tcgtaaagga gaaagaaaat gcgcaaagct caaagacatt tgctgattaa acaagtcatt
     3661 gaagaattcc cgatccgtac gcaagaagaa ctattaaaca aacttgggca ttatggcgtt
     3721 accgccacgc aggcaacgat ttctcgagat atccgtgatc taaaaatcgt gaaggcacct
     3781 gatgaaaatg gcgtttcccg cttcgtattg tttcgcgaaa aaggaactcc tgagactagg
     3841 actgaggaag aggatcggct ggttcaaatg attgaagaca ttgtattgaa agtcgaacga
     3901 gtccactttt tgacgatcgt caataccttg ccagacaatg ctcatttatt tgcatctgtt
     3961 ttagatgaga tcaaacctcc ctctattgtc agtacaattg ctggattcga tacgatcatg
     4021 gtcatctcgg aaaatgagga ggacgcaaaa cgagtagaag aatttcttca ccatccaact
     4081 gaagtatcac ttttttaatc agagcacgta ttcctaatat ggaatgcgtg tttttttatt
     4141 ttcaatattt tccttattaa ataaaccttt acactcttta caaaaataca tcatttttta
     4201 ttcaattttt tcaacacctt gattgaatca caataatcat catttatatt ggatgttaac
     4261 aagagcagct tcttgctctt tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat
     4321 aaattattaa ctcctggacc attgacgact tctgaaacag tgaaaaatgc tatgctgatc
     4381 gatcattgta cttgggatca agaatacaaa gaaattacac aatggataag aaaaaaatta
     4441 ttggatcttg ctcaagtatc ggaaggaata tacacaacgg tcttaatgca aggtagcggc
     4501 tcctttgggg tagaatcggt attaagttct gtgatcaggc cagaagatac tgtactgatt
     4561 tgttcaaacg gcgcatatgg acaacgaatg atcaacatgt gcgaatgtct gaaactgaac
     4621 tataccgttt atgaggtatc gtaacatcaa atcctccaag ctcatgtgat tacacagctg
     4681 attgaagaga atccctctat ctccgcacta gcaatggtcc atagcgagac tacttccggt
     4741 attttgaata atttggaaga gattgctgct gtcacacaag aaaaagatct tttgtttata
     4801 gtggacgcga tgtcaagctt tggtgggata ccgattcgtg tagccgaact agggattgat
     4861 tttttgattt ccagctcaaa caaatgtatt caaggtgttc caggcttttc ttttgtactt
     4921 gctaataaag cggaactaga aaagcgaaaa aatgtgagtc gaagtttgtc acttgatcta
     4981 tatgaccagt accgcgtgat ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc
     5041 catacggttt tggcttttca acaagcgctg aaggaactag aggcagaagg aggaatcgaa
     5101 actcgatatt cacgttatct cgccaataat catgtactac gacaaaaaat gacagagatg
     5161 gggttccagc ctttcattca agaatatcag gggccattca tcacctcttt tctttaccct
     5221 aaggactggc gatttgattt taatcatttt tatcagtatt taaagcaaaa cggatttgtg
     5281 atctatcccg ggaaaatcag cgatcatgaa tgttttcgta tcggaaatat tggcgagatc
     5341 tatccggaag atattttgga actgacagca attatcaaag actataagga gaacgttaat
     5401 gattaaaact gtaatttttg attgggcagg aacaactgtg gactttggct gtatggctcc
     5461 tgtccacgct tttagaaatg cttttctaga aaaaggaatc caactgactg acaaagaaat
     5521 ccgcgaaccg atgggaaaat taaaatggga tcatatccaa cagcttcttt ccctcccttc
     5581 tgtcaaaaaa caatggatag ctatttatgg acaactacct caggaaaaag aggtggagga
     5641 attgtatcaa caatttgaac attatctgtt taaagatttg gtcaagcatt ctaccttgaa
     5701 acccgacaca ttggaaacga tcgaacggtt gagaaacgct gggatcaata ttggttccac
     5761 gactggattt actgctccta tgatgacaat cgtagcagaa accgcaaaaa aagaagggta
     5821 ttcaccagac ttcatggcaa cttctgaaga cgtcgaaggg tatggtcgac cgtttcctta
     5881 tatgatccat aaaaacatgc agtacttcaa aaacaagtcg gttgatgaag taatcaaagt
     5941 cggagatacg gtctcagata tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt
     6001 aattgaaggt agttcgctaa tggggatttc ttatgatgag taccaaaaat tagagatggg
     6061 gaaaaaagat cgtttaaaac agtctgttgc tcttagattc caacaagctg gcgctgatat
     6121 ttgtgtaaac aatttaaaag agttaacaga atacattttg tacgctgaca agaatccaga
     6181 atgactttta tacaaaatca gaggtcgtga aaaagctgta ctgcatcaag taataagaac
     6241 agccaaacaa aaatagctcc tcatattttt cgtttggtta ggcttattac cgcagatgca
     6301 agcttttgaa caccgtttat tcgaaaaaaa aagggagctg tgacacgact tttgtcacag
     6361 tccccttatt tacaaacaaa cagtgtttaa gagacaacct ctccactaaa cggtgttcat
     6421 tcagctgact ctcggtattt gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt
     6481 tgtttctcta atactcggat cagaacgctg atttcacatt ctctttttct tacatcttat
     6541 ctggtgcatg aagtcctagc aaacgaaggt cttctttcaa taatacagtc actgcataaa
     6601 ctagagctaa gcgtgcttct ttttgttcat catcagctaa aatcttcgta tgagcatagt
     6661 atttgttgaa tgcttgtgct aatttgattg catgtttcgc aatcactgac ggttcgtatt
     6721 tttccccagc agacaagacc gtttcagggt atttttgaac taatttaacc acttcccagc
     6781 tggtatcgtc attcaatgca tagtctgctg tttctgaagg gacaaatcct gctttttcca
     6841 acaggctgac tgcacgagca tgtgtatatt gcacataagg tccagtttct ccttcaaatc
     6901 gaacaacttc ttccaggtta aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga
     6961 aaatgactgc cccaacccca acttgtttag ctacagcatc tttgttttcc aaatcagggt
     7021 ttttctcgct gatttgttct ttagcagatt ggattgcttc atttaatact tcttctaata
     7081 agacgatctt tcctttacga gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg
     7141 gaatatggcg catatcgtca gaccagtcaa agcccatttc cttcaagaca gctttcaact
     7201 gtttaaaatg atagctttgt tcattcccta ctacatatag agattgtttg aagtcgtaag
     7261 tacgtttacg gtacaatgca gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg
     7321 acttcttaat caacgctgga ttcaggtcat aagcagatag gtcaacgatc tctgcacctt
     7381 tatcctcgtt cagcaaatgt ttttcttcta aaagtttgac gatttcatcc atcttgtcat
     7441 tgtagaaagc ttcaccgttc aacgaatcga aacgtacttc cagcagatca tagattttat
     7501 tgaattcttt catcgattca tcacggaacc attgccataa ttgaatagcc tcttcatcgc
     7561 cttcttccag acgtttgaac caagcacgag cttcttcatt taattctggt tctgtttcag
     7621 ctacttcatg gaattggaca tataaacgaa gcaattcatt gattggttct gctttaacag
     7681 cttcttctgt accccatttt ttgtaagcga cgatcagttt tccaaactgt gttccccagt
     7741 cacctaaatg gttgatgcgg attggctgat aaccgatttt ttccataatg aaaccgatcg
     7801 aattaccgat aactgtcgaa cgcaaatgtc ccattgagat tggtttggca atattaggag
     7861 aagacatgtc gattggtact gttccttgat tcccgatatt gctatcccca taatgttctt
     7921 tttctttcac tactgtttgc aaaacttttt tgctgataag ttctttattc atgaaaaagt
     7981 tcaagtaagg tccgacaact tcgatttttt caaagttggc agaatcgatt ttttctgcta
     8041 aatctgcagc gatttgctgt ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa
     8101 atgcaacatc tccgtgttca gctgattttg gattttctag taattgttcg acttgttcca
     8161 gtgttaaatc atctttgaca gcgtcataga cagcttttgc gacaagatct ttattattca
     8221 tctttattcc tcctaaagtg ctcccttgat tgttaaacaa aaaaaatctc tagcgacggc
     8281 atacttagta tgccatcact agagacgaga tttcccgcgg taccactcca attgtcctaa
     8341 aaaaggacca gctccatctg atatcggtag acgaccgtct gtttaaaaca gaattctcca
     8401 agtgcgcttc gttgtatctt cttcattcgg cttccaccac ttccgaattc gctagccaag
     8461 aatgatacaa gtactctctt gttcaacaaa tcttagtctt attaaacttt cttgaattat
     8521 atcagaaaaa aaaacgaaag gctagtcgtt tctgataaaa aaacaatcca gatggcctaa
     8581 aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa agacttaaca
     8641 aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc gatattttct
     8701 aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat agctgcctct
     8761 actttcggaa gcatgcttcc tggagcaaat tgcttttcat caatgtattg tttcatttct
     8821 gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac atacacattg
     8881 tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa tttttctgaa
     8941 gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt ttctacaaca
     9001 ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag cgtttcgatg
     9061 acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct ccatccacgt
     9121 ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt ggcttcttct
     9181 tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc attttcatcc
     9241 acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc cttcagtact
     9301 tcgcccatgg cattttgtaa ccagtaaccg atagatcctt ctgtcattgc aacacatgta
     9361 tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat caataggtta
     9421 ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc gataaatttc
     9481 actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact tgcatccgta
     9541 gacaagattg cattaccccc taaagcgaca actacttttc gtttggccat ttcattcgtc
     9601 ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt tgtgattttc
     9661 cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac gcgagggata
     9721 aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg attttctgct
     9781 tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc catttcagtg
     9841 atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc
     9901 aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat ttccatattg
     9961 atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc acccattgat
    10021 acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac atcgtcggta
    10081 atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac gatttcttct
    10141 tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg
    10201 actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac agtgatacct
    10261 tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg tgttggatgc
    10321 cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag ttcctctacc
    10381 attttttgac tgaatccacg gaattcgatt ccatcaaaca tacgacctag tactttagct
    10441 gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag atattcagga
    10501 tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt agatgttttt
    10561 tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc acgttttttc
    10621 aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt tgtaaagtct
    10681 ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa cattctcctt
    10741 tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca gtaagaccgc
    10801 cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat gcagcgtgga
    10861 ccaccacggc ctcgtgacaa ttcacttgaa ttgatttcta acactttgat gccatagcta
    10921 cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc tggagcgatt
    10981 gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc atctccatta
    11041 ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag
    11101 ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa gacatccatc
    11161 ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc taccattgta
    11221 aatacggtat ccaaatgcat cattgcacgg ttgtttggaa ttttgattgc taacactttt
    11281 tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc tttcgcatta
    11341 gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc gtctccgcct
    11401 tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt tgcaaatctt
    11461 ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc
    11521 atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag
    11581 tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc gtatagactg
    11641 ctagaacgta cttcaatatc ttttgtacga accccagcca tgattttatc taccatttct
    11701 tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt cgatgcaata
    11761 tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc gtcaatcgct
    11821 tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa tgtctttgca
    11881 aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa caacagacga
    11941 tccataatat ctggtgtcaa attttccact tcttgtccag gccttttcag taaaactgtc
    12001 tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt
    12061 ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa ttttgtctca
    12121 ttca
//
LOCUS       DAXWLF010000077        12041 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXWLF010000077 DAXWLF010000000
VERSION     DAXWLF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.077"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..261
                     /locus_tag="ACGE4U_001583"
     CDS             <1..261
                     /locus_tag="ACGE4U_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFF8393753.1"
                     /translation="WIFDRSRNASIGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSS
                     TLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(352..717)
                     /locus_tag="ACGE4U_001584"
     CDS             complement(352..717)
                     /locus_tag="ACGE4U_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393754.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            925..1407
                     /locus_tag="ACGE4U_001585"
     CDS             925..1407
                     /locus_tag="ACGE4U_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393755.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1604..2494
                     /locus_tag="ACGE4U_001586"
     CDS             1604..2494
                     /locus_tag="ACGE4U_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFF8393756.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2820..5294
                     /locus_tag="ACGE4U_001587"
     CDS             2820..5294
                     /locus_tag="ACGE4U_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8393757.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5397..7133)
                     /locus_tag="ACGE4U_001588"
     CDS             complement(5397..7133)
                     /locus_tag="ACGE4U_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8393758.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7130..7834)
                     /locus_tag="ACGE4U_001589"
     CDS             complement(7130..7834)
                     /locus_tag="ACGE4U_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8393759.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7965..8468
                     /locus_tag="ACGE4U_001590"
     CDS             7965..8468
                     /locus_tag="ACGE4U_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFF8393760.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8428..8766
                     /locus_tag="ACGE4U_001591"
     CDS             8428..8766
                     /locus_tag="ACGE4U_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFF8393761.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8787..10205
                     /gene="ffh"
                     /locus_tag="ACGE4U_001592"
     CDS             8787..10205
                     /gene="ffh"
                     /locus_tag="ACGE4U_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFF8393762.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10317..10895
                     /locus_tag="ACGE4U_001593"
     CDS             10317..10895
                     /locus_tag="ACGE4U_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFF8393763.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10899..11420
                     /locus_tag="ACGE4U_001594"
     CDS             10899..11420
                     /locus_tag="ACGE4U_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFF8393764.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11499..>12041)
                     /locus_tag="ACGE4U_001595"
     CDS             complement(11499..>12041)
                     /locus_tag="ACGE4U_001595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFF8393765.1"
                     /translation="IGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLPFYNE
                     DLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHSVWDH
                     KPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNIVEGT
                     LSFFQTILDAYLDFSNKLTE"
BASE COUNT         3950 a         2157 c         2487 g         3447 t
ORIGIN      
        1 tggatttttg ataggagtag aaatgcaagt ataggtttta gaactaaacg aagtctgtct
       61 tctaaaaaaa attgggtata ctcacaaact attttttatg gaggaattgt tttaattagt
      121 ttactttctt caacacttta ttctttgaat attatagatg tttccacctc aaattcaata
      181 tctattattg gaatcattat tgctgcaatt attacacaac tgtttttagt gtttggagag
      241 aagaaaagaa gtaaaaaata gaaaaactag tgatagtttt ggtgatataa gaaaaacata
      301 tatgtataaa aagaagaaag tagcttctct ttgtacatat atggtttgtt tttatggatg
      361 ccgagctttt ttataagata aggctaccgc ccataacgac ccaaggattt gtggaactaa
      421 gtaaatacct gcaagaagca agatcagctg ggtcggtttt aaaggatgat agaagtaacg
      481 ccattcagta aaattggcgc aaaataaata ggaagtgaca gtagatataa tattggcaat
      541 gaataaagat ataatacgcc tgttcctttg aaaataatat ccagcaaaaa gagataaaaa
      601 tatcaatgtg atgaacccgc ttacggaatg ataacgaaaa tcaagcagta aggcgaaatg
      661 cacataagga acaaaagata aaagcaacgg aaggtttttc cagcaagttt ttatcatctt
      721 tcatcaccac ctaacaagtg ttattatttt gttagtttta ttgtaaattg aatcctgcaa
      781 taatcaatta ctttttcatt atttctagat taaatgaata aactctcatg ccattatctc
      841 taaaaaaaaa gtttttcccc ctaatttttt tagatagaaa attgtatagt atgatgagag
      901 aaaaagtagt ttgggggaac gttaatgaga agaaaagcag taatcgatcg gctttattta
      961 tttttttggt tttttcctat cgttttgcta ccttttacat gggtaaatgc tgttgtacaa
     1021 ggtgcggaga tattaggcct gatccaagga agcggattag tggtttctag gaattttcca
     1081 gctttgactg ttttctattt agtgataagt gtaggtctct tattaccaaa atctctgatc
     1141 aatcactaca attggcagct gctttacttt tcttgcttat tagtatttac atctatgttt
     1201 tctttgctgc ctcgcttgat tatcggccct aatggtttga gcgagagtgg attgaaggat
     1261 tcagcttatt cctccatgta tttcttgacg atcaaaccat ggttctattt agtaattgcc
     1321 tcatttattc ttgcaacatt tttttacttc tggacagaaa tcaggataaa aagtgaacga
     1381 tctgtatcta taaaaaataa ctattgatgg tggatcatca gtaaaaaagc atatgagctg
     1441 cgtaaaatgc atgtttctca tatgcttttt ttattattat gtaaagaatg cgaaaaacgt
     1501 ttacatgatt tttacataac ttaactatat ttttacagca accatacgag ggatttacag
     1561 tcgactgata aactttagtt gtaaataaga aaggagcaat aaaatgaaaa aagtattgtt
     1621 tactctaggt atgatgggaa ttcttttagc cggatgcggc tcaggatcag gaaatacaga
     1681 tggctctgcc acacaaaata attcaagtga ctcaaatgaa caagtaaaaa tcgttgcggt
     1741 aggttcgaca gcattacagc cgttagtcga tgcagcacaa gaaagctttg tacaagaaaa
     1801 tccaaattat caaatctctg tacaaggagg aggaagcgga actggtttga gccaagtcga
     1861 agctggagca gttactatcg gaaactctga tgtatttgca gaagaaagag acggagttga
     1921 tgcttcaaaa ttagtggatc ataaagtagc agtggtcggg atggcaccaa tcgtcaataa
     1981 agatacagat gtcaaagata tcaccaaaca ggaattgatt gatatcttta caggaaaaat
     2041 cactaactgg aaggaagttg gcgggaaaga tcaaaaaatc aacgtcgtga accgtgcaaa
     2101 cggcagcggt acccgcgcga cattcgaaaa atggggactg gatggtgcta cccctgtgca
     2161 gtcccaagaa caagattcat caggaacagt tcggcagctg gtaagtcaaa caccaggagc
     2221 aatcagctat ttagctttct catatctcga tgattccaca caagcactaa gtatcgacgg
     2281 tgtagaacca aaagaagaga atgtagcaga taacagctgg ggaatctggt catatgaaca
     2341 tatgtacacg aacggaaaac cttcccctga agtccaaaaa ttcttagact acatgatgac
     2401 agaagaaatc caagaaggac ctgtcaaaga attgggctat ttaccaatca caatgatgga
     2461 agtagaacgt gatcacgaag gaaatatcaa ataactagca gataaacatg ccacggtagt
     2521 cgttactatt aaggaaaaat caactatgtt tccccacata tacatttttc ctttaattgt
     2581 gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga
     2641 acaccgttta ttcgaaaaaa agagctacga catagaactg tcgtagcttt ttttttgcta
     2701 tatgacagga atgaaaggtg tttgcaagct taatgtgagg aacactttcc atatctgttc
     2761 tatactggat aggagaagtt tggaaatatc cagaaaacga aatcaatcga ggtagccaca
     2821 tgaagaataa atggataaat cgaagtaaaa aaaagaaagt aacacaaaga tcatcagata
     2881 aagtcatccg tctttttcca tcgtataaag aaggattaac ggcagatcaa gtcaaggaac
     2941 gaatagaaaa aggtgcagca aataattcag tcgatcccac attcaaaaca aatcagcaaa
     3001 tcgtattgga aaacatattt acgtatttca atttgatctt tcttatctta gccattttgc
     3061 tttgtttagt agaatcctat aagaacttga cttttttacc agttatcata gccaatacag
     3121 gaatcgccat ttttcaggaa attcgatcca aaaagatctt ggacgaacta aatgttttaa
     3181 atacagcaaa agtcaatgtc atacgagacg ggatagaaca aacaatagat atagaagacc
     3241 ttgtaataga tgacattgtt attttagaga ctggacatca aattccagca gatgcagagg
     3301 tcgtggaagg gaaactgcaa gttaatgagg ctttattgac aggcgaagca gatgaaataa
     3361 ccaaagaaat aggcgatcca ttaatgtccg gcagtttcat tgtttccgga aaagcttacg
     3421 cccgattaga caaagttgga gcagattcgt atatttccca gctgacagtc aaagccaaga
     3481 caatgggaga aggagaacag tcggagatga ttgcttctct taatcaattg atcaaatgga
     3541 ttgggatcat cattattcct atcggtatcc tgcttttttc tcaaagttac ttttttaatg
     3601 gaaacacgat caaagaaagt gtcgttgcaa tggaagcagc attgatcggt atgatacctg
     3661 aaggactgta cttgctgacg acgattgcat tagcattaag tgcgactaaa ctagcaaagc
     3721 aaagagttct tttacacaat atgaaaagca ttgagacatt ggcaagggta aatgtccttt
     3781 gtgtagataa aacaggaacg atcacagaaa ataaaatgtc tgttcaaaaa gtgatcgtct
     3841 ctaaaaaaca agaaataatc agtcagtcag atcaattgga agcatgcatt gctgactatg
     3901 caaaagcgat ggcaggagac aatgcaacaa tggaagctgt taaagcatat tttacaaaaa
     3961 ccactgatca atcctatcac catgttattc ctttttcttc tgtccaaaaa ttcagcagtg
     4021 tagcgttggc agacaaggtg tatgttttag gagcgcccga aatggttttg cgcgatcaat
     4081 tgccggaata tgcggaagag tttgtttctt tcgctgaaaa aggctatcgc gtgttggtct
     4141 ttggtgtgta cgatgggatt cttgaagaac cagtattgac agaagcagtc acaccattag
     4201 gctatattct gattgctaat cctatcagaa aagaagcaag agccacattt gattacttca
     4261 aaaaacagca agtagccatc aaagtgattt ctggagataa tccgctcacc gtatccaatg
     4321 tagctgtcca agcagggatt tcacatgctg atcagtacgt tgatgcatct aaattatcag
     4381 aagaagaata tgaagcagct gtcgaaaaat atacagtttt tggacgcgtc aagccagaac
     4441 aaaagaaaat cttcgttcaa ttgctaaagc gtcgtaacaa tacagtagct atgacaggtg
     4501 atggcgtgaa tgatattcta gccatgaaag aagcagactg cagtatagcg atggcttccg
     4561 gaaatgaagc cgccatgcag gcctcacaag tggtcttact tgattctgac ttttcacgca
     4621 tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga tcagccagct
     4681 tgtttttagt gaagaatatt ttctcctttt tgctctcact gttttcagtg atctttgctc
     4741 tgacctatcc tttagaacca tcacaaatca cattgattag tttgtttact atcggacttc
     4801 catctttctt gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa ttcatcatga
     4861 atgtgatgga aaaagctgtg ccaggtggac taacagatat gatcgtagtc ggtgcgttgg
     4921 tgatctgcgg ggtgactttg aatctaaata aaacagatgt ttctacagca tctaccatgc
     4981 ttttgattgc agtagggttc ctcgttctct ataaaatctg ttcaccgctg aataaattca
     5041 gaagtcagat cattcttttc tgtgctagcg gtaacttttt ttcggtcatc tttttgcata
     5101 agctgttttc aatcaccggt atctcagctg tatcactttt gctgttgagc attctatttt
     5161 tctcggcaga ttcgattttt aggcatttga ccacattggt agaatatttg tttgaacgta
     5221 aaaatgaaaa agagcaaaag ccgtttagtc gcgtagttaa aaaatttcta tcgatattta
     5281 aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac cactcgtgta
     5341 tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc tcttttttac
     5401 attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc
     5461 gtaccatgaa ggagttgcac gtagtctttt acaatcgcta accctaatcc ggtcccgcct
     5521 gaatgccggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg ttcctgatcc
     5581 tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc tgttgcctga
     5641 tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc aatcagattt
     5701 ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt tccaatcaat
     5761 cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat ttgctggaaa
     5821 taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt tgacagctgg
     5881 atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa
     5941 gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt aaaaccaatc
     6001 agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc cttttgtagc
     6061 ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg tccggtacct
     6121 tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc tgtaagggtg
     6181 atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg aatgagtttt
     6241 ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac gtttaaatgt
     6301 agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat caggaatacg
     6361 ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt atacgcccga
     6421 tacatcttac tcatttgttc gctcaacgca ttgatcgtct gatacaattc ttcccattga
     6481 tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt ttttagtacg
     6541 ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat cattcctgca
     6601 agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact ctcagttctc
     6661 ggtaaaaaac cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg gccacttttt
     6721 ttgactggta atgcgacata taaaagatcc ccattcaaag tagtactttt gcgcaaagcg
     6781 ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt tcgctgttca
     6841 ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat taaagtaatc
     6901 cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc tgcttggagg
     6961 ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc
     7021 tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa
     7081 ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt catcgtttgg
     7141 gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg tgttttggat
     7201 ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg
     7261 cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca cggtcttttc
     7321 gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc agatggtcgt
     7381 ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat agatattcag
     7441 gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt gctttcatac
     7501 gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca cctatttcta
     7561 ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc ggtgtttcat
     7621 ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga agcatcacat
     7681 ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg cctccatctg
     7741 tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc gttacaatag
     7801 aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa taggtttcat
     7861 tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag aaataaatgg
     7921 tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg
     7981 catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta ccagaattga
     8041 tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc ggcggattac
     8101 caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg tggaaagatc
     8161 aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa aatggcgcga
     8221 tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg aaggaaaaaa
     8281 gcccttcatg cggaacccat tcgatttatg acgggagttt ttccggaata aaaaaaaatg
     8341 gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc tctgatgaag
     8401 aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg
     8461 ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat ggaattatat
     8521 tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag ccgacaagca
     8581 gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa aaaacttcat
     8641 cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc atatattaca
     8701 gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca agcaatcgaa
     8761 gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa cgtctccagc
     8821 aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta aaagaaatga
     8881 tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta gtcaaagatt
     8941 ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt ttatcacctg
     9001 cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt tctgaaacag
     9061 cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt cttcaagggg
     9121 caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat gaaaatgctc
     9181 gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat
     9241 taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc ccggtagaga
     9301 tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata
     9361 cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa atcaaagaat
     9421 tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa gatgccgtca
     9481 acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc actaaacttg
     9541 atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc gcaccaatca
     9601 aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca gatcgtatgg
     9661 ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg cagcaagact
     9721 atgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca ttcgatttca
     9781 atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa gatctaatca
     9841 aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag
     9901 atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga gcaaatcctg
     9961 atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg
    10021 aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag caaatgtcca
    10081 aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac
    10141 taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga
    10201 aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg gagcttttct
    10261 atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg gcacagatga
    10321 gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact gtctggggtg
    10381 ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt aatcaagccg
    10441 gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat
    10501 tcgatattaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta atggaaaaca
    10561 aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct
    10621 tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt tctaatggaa
    10681 aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt cctctttcag
    10741 atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc acaacaatct
    10801 atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt ttccgcaaag
    10861 aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc atattttatg
    10921 gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa tggctttttg
    10981 acactccaac aggtaagcaa accacttatg aagcttttta tgaaacaatc gatacgacca
    11041 tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa gcaatttatc
    11101 cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca gatgctgagg
    11161 tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa ggaaaagata
    11221 tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa atgattgatg
    11281 aatggtatat ccagattacc cctgtattat taggggacgg gattccttta ttccaaaaag
    11341 gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa tttatcgaat
    11401 tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat gcagaaatgt
    11461 cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa tttgttagaa
    11521 aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc aacgatatta
    11581 ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt
    11641 ggcacattca aaaagacaag tgattgtcgt agatggtggt ttgcgccaaa gccgccgatc
    11701 ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact gtgtccataa
    11761 ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg gttgtattca
    11821 ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg agtccattca
    11881 gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc atcgattttt
    11941 acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc tacttttttg
    12001 ttgtaagaat cttttcttaa actaccaata aaaaatccaa t
//
LOCUS       DAXWLF010000078        11956 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXWLF010000078 DAXWLF010000000
VERSION     DAXWLF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.078"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            73..711
                     /gene="plsY"
                     /locus_tag="ACGE4U_001596"
     CDS             73..711
                     /gene="plsY"
                     /locus_tag="ACGE4U_001596"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFF8393766.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(843..1718)
                     /locus_tag="ACGE4U_001597"
     CDS             complement(843..1718)
                     /locus_tag="ACGE4U_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFF8393767.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1800..2591)
                     /gene="codY"
                     /locus_tag="ACGE4U_001598"
     CDS             complement(1800..2591)
                     /gene="codY"
                     /locus_tag="ACGE4U_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFF8393768.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(2609..4009)
                     /gene="hslU"
                     /locus_tag="ACGE4U_001599"
     CDS             complement(2609..4009)
                     /gene="hslU"
                     /locus_tag="ACGE4U_001599"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFF8393769.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(4023..4571)
                     /gene="hslV"
                     /locus_tag="ACGE4U_001600"
     CDS             complement(4023..4571)
                     /gene="hslV"
                     /locus_tag="ACGE4U_001600"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFF8393770.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(4608..5513)
                     /gene="xerC"
                     /locus_tag="ACGE4U_001601"
     CDS             complement(4608..5513)
                     /gene="xerC"
                     /locus_tag="ACGE4U_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFF8393771.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVIYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(5711..7087)
                     /gene="trmFO"
                     /locus_tag="ACGE4U_001602"
     CDS             complement(5711..7087)
                     /gene="trmFO"
                     /locus_tag="ACGE4U_001602"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFF8393772.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(7056..9134)
                     /gene="topA"
                     /locus_tag="ACGE4U_001603"
     CDS             complement(7056..9134)
                     /gene="topA"
                     /locus_tag="ACGE4U_001603"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFF8393773.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(9256..10113)
                     /gene="dprA"
                     /locus_tag="ACGE4U_001604"
     CDS             complement(9256..10113)
                     /gene="dprA"
                     /locus_tag="ACGE4U_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFF8393774.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(10169..10936)
                     /locus_tag="ACGE4U_001605"
     CDS             complement(10169..10936)
                     /locus_tag="ACGE4U_001605"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFF8393775.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10929..11789)
                     /gene="ylqF"
                     /locus_tag="ACGE4U_001606"
     CDS             complement(10929..11789)
                     /gene="ylqF"
                     /locus_tag="ACGE4U_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFF8393776.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         3099 a         2623 c         2101 g         4133 t
ORIGIN      
        1 ttttttcaat tgttcctgtt acctattcat gctaaaataa aggatagttt tcaacttgaa
       61 agagggtaaa aaatgaaatt gatcttatta cttattttag catatctttt aggttctatt
      121 ccttccggtg tctggattgg caaattgttt ttcaaaaaag atatccgaca acacgggagc
      181 ggtaatactg gaacgaccaa tacatttcgt gtattaggca aaacagccgg gattgttgtc
      241 ctgcttatgg atattttaaa aggtacattg gctacttgtt tgccgatgat cttccacctt
      301 tctgggatta accctctttg gtttggggta tgcgccattc ttggccatac atttccgatt
      361 tttgctgggt tcaaaggagg aaaagctgtt gcgacaagtg caggaatgct tttaggattt
      421 aatcctgtat tttttgttta ttcttgtatc atctttatcg ttagtttatt ttgtacaagt
      481 atggtgagtt tgagcagtat gatcagtgca gtattgatca ctttatctac gatcgtcctt
      541 ccttatgtag ctcctgtgat tttggctaag cccaattggt tactgacgat cattgccttt
      601 ttagtttctt cctttatctt ttatcggcat aaagacaata tcaagcgtat cagaaaccat
      661 actgaaagtc gaatcccgtt tggtttgaat agcagcaaga aaaaatcctg atactttttc
      721 attccaaaga gataaaacgg gagtgtgaca aatcgatgtc acactccctt attacttaca
      781 ggatagcttt tttacacacc ttggtaaacg gtgttccctg ctaacttctc ggtcacaacc
      841 tcttacttca ctgtgatgga atattttgtc ttgaagattt catgggcacc tagttgattg
      901 atccctactt tttccatcaa gtttccacta ctgttcaccg tgtcagcgat tccgcaccac
      961 ggttcgatac aaacgaaagg cgcttctttt ggataaggag accagatccc tacataaggc
     1021 atatctttat agcttaaagt tacgctatgg gaagattttt cacttcgaat ggcgtacgca
     1081 tttagacccc ttgtttcaaa aaccaaggca tcttgctcaa aaagttcacg attcaaagcc
     1141 agattcgtat ttgtctgacc taacgtcttt tgatccatat cgatgaatgg gcctttcaat
     1201 ggtaggcgta atcttgattt caatggagaa aatgccagat agaaatcttc aaatgacaat
     1261 ccttcttcaa gaggtacatt gaaagcagga tgtccaccga tagaaaagaa catttctttt
     1321 tcaccggtat ttgccacttg ataatgaacc gtgatgccgt ctcctcctaa ttcatagctg
     1381 atcactaatt caaaatcaaa tggatacttt tctaatgtct ccggcgtact ccttaagatg
     1441 aaactagcca tttcttgtcc atgttcaatg acttcaaaat ccatatcccg agcaaatccg
     1501 tgttgcccca tttgatatgt tttcccttga taaacatact gatcatcttt taaacgtcca
     1561 acaaaaggga ataaaaccgg tgcatgtctg ccccaatacg ctggatcacc ttgccagata
     1621 tactctaaac catgttcttt tgatttcaga ctgatcaatt ccgcaccgtg ttcagcgatt
     1681 tcggcgatca gctggtcatt ttgaataata acagtcattg ccattcctcc aacttcgact
     1741 tactttttaa aatgggaagg ctggaacaaa catttctatc tgccccagtc cccttacttc
     1801 taataactgt gttttgctaa ctcgtcttta aaacgggaat tcaatacttt taaataagtt
     1861 cccttcatgc ctaacgaacg agattcaatg attccagctg attccaattt tcgtaaagca
     1921 ttgacgatca cagaacgagt gatgccgata ctatcggcaa tattagaagc agtcagacga
     1981 ccttcatctc catccaatgc ttcaaagata gcttgtacag ccttcaattc gctataagat
     2041 aatgtgttga ttgccatttg gacagccgtt gcactccgta catctgcttc gatgcttcga
     2101 gacttctgat aaaggatctg cataccgaca accgtagctc catattctgc taagactaga
     2161 tcgtcatctg caaacgattg ttccatacga gataagataa tcgtacccaa acgttcgcca
     2221 gctccaaaga tcggaacgac agtcgtcaga ccaaatggat atttctctct tgactctacc
     2281 ggaaatgcag taatatcact agtaattggg atattagctt cagttttcat caactgtgcc
     2341 actgtatcag tataagattc aggaaattgt ttctcaacaa acattgtttt gattcgttca
     2401 ttgttcacat cgtgacgttc attataacct agtaatactc cgtcacttcc tatgatatag
     2461 gtgttgctgt caagaatacc acctagtgtg acagccattt tatcgtaagg caaatctgct
     2521 tgcatgtcaa aagtattctt ttgctgcaaa agtgtgttta tcttgcgggt tttcgttagc
     2581 aagtctgtca ttgtcgtcct ccgatttttt ataaaatata gcgacttaaa tcttcatttg
     2641 ccgcaattga gccaagtttg tcatccacat atgcttctgt gatcgttatt tctcccattt
     2701 gcatatctgg tgcttcaaat aatagatctt ctagtagttt ttccaaaatg gtatgcagtc
     2761 tgcgtgcgcc gatattatct gtgtcacggt tgacatcgaa agcgatttga gcgatccgtt
     2821 caattgcttc ttttgtaaac gtaactgaca cattttctgt accgatcaac gcgacgtatt
     2881 gttttactaa agcattattt ggttcggtca gaatcttcac aaaatcttca gctgtcaaat
     2941 catctaattc tacccgtatc gggaagcgtc cttgaagttc tggaattaga tcacttggtt
     3001 tagacaaatg gaaagctcct gaagcaatga ataggacgtg atctgtttgg atgatcccgt
     3061 attttgtgtt tacttgtgaa ccttcgacaa ttggcaatat gtctctttgg acgccttctc
     3121 tggatacttc tccagattgc tgtgatttag aagtgatctt atcgatttca tcgataaaaa
     3181 taattccact ttcttccgct aatttgattg cctcactatg gatatcagca tccttgacta
     3241 attttgctga ttcttccttg atcaataact ctaaagcttc tttgactgtt accgtccgtt
     3301 ccactttttt cttcggtgtc aaagcaccta gagtttcatt taggtcgatc cccatttgct
     3361 ccaatccatt gttcatcatt ggtgcaggtt tcttttggtc ttcaacttca atggttactt
     3421 cacgactatc cagtaaaccc ttttgtagtt gttccaagac tgtacggcga ttgattcgaa
     3481 tatcttctgt cacttcttct tgcggttcag cggtattttg ctgactagca ttaaaaagat
     3541 tcatcatttg ttcaaattga ttagtggatt gtttctgttc ttttttgata ccaggtacca
     3601 agatttttac caaacgtttt tctgcatttt ttttagcact tgtgtataca cggctgtact
     3661 gctctttttc tacgatttgg atcgcatttt ctactaaatc ccgaaccatt gattctacat
     3721 ctcttccgac ataaccgact tcagtaaatt ttgttgcttc gactttgaca aatggtgcat
     3781 tgacgatttt tgccaaacgt cgtgcgattt ctgttttacc gacccctgta ggtccgatca
     3841 tcagcatatt ttttggtgta atatcttgtt gcattttttc atctaactgc aaacggcgat
     3901 agcggttacg caaagcaacg gcaacagatt tttttgctgc gtgctgtcct acgatatatt
     3961 catctaattc acttacgatt tgtcttggtg tttgttttaa ttcatacata tctactcacc
     4021 tcttacagtt cttctacgat aatattatga ttagtaaaga tacagatatc tgcggcaata
     4081 tttaacgcat tttctgcaat ttctttcgca ctcatgtcgt tttgagcata gtttttcatt
     4141 gctcgggctg cagccaacgc aaaattccct cctgatccga tagctaagat accatcatct
     4201 ggtgtgataa cttctcctgt acctgaaaca agaagcattt ctttatcatt cattacgatc
     4261 agcatcgctt caagtttttg catagattgc tgtgtccgcc attcttgagc taattcgaca
     4321 gctgcgcgtt gtagattccc gttatattcg tttagtttgc cttcaaattt ttcttctaaa
     4381 gtaaacgcat ctgcaacact tccggcgaaa cctacaacaa cttctccatt gtagatacgt
     4441 cgtactttct ttgcagtgcc tttcatgaca acagattcac ccattgttac ttggccatct
     4501 ccagccatag caaatttccc gtctttttcg acggcacaga tagtggttga atgaaatggt
     4561 gattcgacca tgtttgtttc ctcctaaatt tgttcttttc cttattttta cgctcttgga
     4621 tggaaagaac gatagttttt ttgtaaactt tcttttgtta catgggcata gatctgggta
     4681 gtcgataaat tggcatgtcc tagcaattct tggactgtcc gcaaatctgc cccattattt
     4741 aataaatgag tagcaaatgt atggcgcagc atgtgaggat ggatatcact atccaaactg
     4801 ctttttttga tcatttgatt caagacatat tcaattccag taggcgtgat tggttttcca
     4861 tgatgattca caaaaacgat atcatgctct tcgtgatatt ttgtcatcaa tacctggcgg
     4921 ccttccctga aatattcttg cagagcatct tgagcaaaag aacctagagg agcatatctc
     4981 tctttgttcc ctttaccatg gatcaacatg acctctgaat caaaatcaat gtctgatacc
     5041 gacaaactgc tacactcact taaacggatg cctgacccgt aaatgacttc cagtaaagcg
     5101 cggttcctca aatccaaagg actatctcct gctacgctgt caaaaagtgc ttgcatttca
     5161 ttttcataaa aaaaacgtgg cagtctcaaa tttttctttt tgagatgaac gtaagaaaat
     5221 ggattttctt ttatcacttc ttctttgaga agatactgat aaaacgaacg taggctggct
     5281 atcttgcgac tgatagaatt tctgctatag ctaggacgat gatcagctaa tgcactcaaa
     5341 tagacacgaa ccgtaaaata ttccgccttc agcagatcag cgtctccaga ttcctccaaa
     5401 aaatgtttga aatggagaat gtcttcttca tatgcttctt tcgttttttc tgaatatcct
     5461 ctttcgacaa tcaaataacg caaaaatttt tgttcccacg tggcttctgt cattttctca
     5521 ccttcctttt gtcccagaga ataatttagc acagcgttta taaaaagaca aataaattgt
     5581 cagaatttaa ccaatatttc taagtttgtt cacaattggc gcaattcctc acattttgtt
     5641 tgagaaattc ttaagaaaaa cgcaagaaaa agagcgagac tatccgtctc accctcttca
     5701 tggagtttcc ttactcattt gggattgcag ttttttctaa ttctgttttt attgcctcat
     5761 tagcttttaa agctcgttgt gcaattgctt catatcttgc ttttttgtct cgaatgcgct
     5821 ctggcagttc aggaaacaga ccatagttgg cattcatcgg ttggaaatgt tttccttccg
     5881 catgagtaat gtagtaagcc atacttccaa tagctgtttc ttgtggaaac tcgactggtt
     5941 ccatttcttt tgctaagcgt gcggcgttga ttccagcaag tagtccgctc gctgcacttt
     6001 caacatagcc ttctacacca gtcatctgac ctgcaaagaa cagatcttcg cgtttcgttg
     6061 attggtaagt cggtttcagc aattctggcg aattcataaa gctgtttcgg tgcatcacac
     6121 catagcgaac gaattcagca ttttctaacc caggaatcat ccggaaaaca cgtttttgct
     6181 cgccccattt caagtgagtc tgaaagccca cgatgttgta taaagaagct gcagcattgt
     6241 cttgacgcag ctggataaca gcataaggac gtttgccagt tttaggatct tccaatccaa
     6301 ctggtttcat aggtccgaaa agcatcgttt tgaatcctcg ctgtgccatt acttcaattg
     6361 gcatacatcc ttcaaaatat ttttctttct caaaagattt aagttctgcc acttcggcat
     6421 tgattagtgc ctcatggaaa gctttgaatt cctcttctgt catcggacag ttcaagtaag
     6481 ctgcttctcc tttgtcatag cgggacttca aatatacttt atccatatcg atcgtagctt
     6541 tgtctacgat tggtgcagct gcatcataga aataaaaacc atctgaacca ttgaatgctt
     6601 tgatgctttc tgctaatgga tgagaagtca aaggtcctgt tgctactacg gtgattcctt
     6661 ccggtatttc agtaatttct tcgtttatca ctgttactaa tggatgctct ttgacttttc
     6721 gggtaattgt ttcagaaaaa gaatcgcggt caacagctaa ggcaccacca gctggcactg
     6781 ctgtttcatc tgccgaagtg attactaccg aattcaatcg gcgcatttct tctttcaata
     6841 accctactgc attagccaaa ctgtttcctc ttaatgaatt ggaacatact agttcagcaa
     6901 aattacttgt gtgatgcgcc tcagtcgatt tgactggacg catttcatat aaacggacag
     6961 ggactcctgc ttgtgcgatc tgccaagcag cttcacttcc ggcaagccct gctccaacaa
     7021 catttaccat tttcaatgaa agaacctctt ttctattatt tttggatgtc ttcttcgtaa
     7081 tctccattta cacagactac ttgtttaccg cctttgactt tcttctcgac cagatatccg
     7141 tcacattttg gacaatttct gcccactggt ttatcccaag aagtgaattc acattctggg
     7201 aaacgggaac aaccatagaa tagtctgttt ttcttagatt ttcgttcaat gacttgtcct
     7261 tcatgacata ctgggcaagt cacaccgatt tcttttacga ttggtttcgt attccgacaa
     7321 tcaggaaaat tactgcaagc ataaaacttc ccatatttcc ctaattttat caccatcgga
     7381 tgtccacaga catcacaatc gaatcctgct ggttcatctt tgatttgaat tttttcgatt
     7441 ttttcttccg cattcgtcag ttctttttca aacggtttat agaaacgatc aaccactttg
     7501 acccacgctt ccgtgccttc ttcaatttta tccaaatctc cttccatttc tgctgtgaaa
     7561 tggatatcga cgatttgagg gaagaattca cagattaatg aattgacaat ttctcctagt
     7621 tcagtcggct caaatctttt ttgtgtcaat tttacatagt agcgtctttg gatcgtatca
     7681 agtgttggtg cataagtgga aggtcgccct accccgtttt cttctagtgc acgaatcaac
     7741 gtagcctcac taaaacgtgc tggtggttga gtaaaatgtt gttttggttc aatatcaaca
     7801 gattggacta catcgccttc ttcaagttct ggaagaatat tttctttctc ttctttgccg
     7861 tcatctctac cttccacata cacttgcatg aatcctttga atttgatttt tgaaccgttc
     7921 gcaatgaaga tgacaccatt ttgttctaat gtcactttca ttgtatctaa aatagctggt
     7981 gtcatctgac tagccacaaa acgtgaccag atcaatgtat aaagtttcaa ttgatcttta
     8041 tctaaatatt gtttgagttc atctggtgta cgaagcacac tcgttggacg cacagcttca
     8101 tgggcatctt gtgcgccttg agcatttttg atctttcggt gatcatgtct agaaaattct
     8161 tttccatacg tatcttcaat aaatgctgcg gcctctgctt ttgctgtatc ggcaatccgg
     8221 gtagaatcag tacgcatgta tgtaatcaag ccgactgttc cttgtttacc caaagtgatt
     8281 ccttcatata attgctgtgc taccatcatg gtttttcttg ttcgaaagtt taacttacgg
     8341 gcagcttctt gttgcaggct acttgtagtg aaaggattcg cagggtttct tttgcgctct
     8401 ttcttttcta cttttttaac ttcgaagtct gttccatcaa ttctagcagt gacttctttt
     8461 acactttgtg catctggtaa tttctttttc ttaccgtcga ttccccagaa atttgctttg
     8521 aatttcttgc gagctttttt aaaattaccg tcgatgctcc aatattcttc tggaacaaat
     8581 tctcggatct ctttttcacg gtcaataata atttttaaag cgacagattg gacacgtcct
     8641 gcacttaacc ctttcttgac cttacgccag agaataggac tgatcgaata accaacgatt
     8701 ctgtctaagg tacgacgagc ttgctgtgca tctactaaat ctacatcgat cgttcttggt
     8761 tccttaaaag ctgctttgac tgcgtctttt gtgatttcat tgaagaccac ccgatttttt
     8821 tctttcagat ccaacccaag taggtacgct aaatgccaag caatcgcttc tccttctcta
     8881 tccgggtcac ttgccaagta aactttttga gctttttttg ctgcggcttt caggcttttg
     8941 atgacatcgc cttttccgcg tatagaaata taatggggtt cataattgtt ttcaaaatcg
     9001 atacccattt tacttttcgg taaatctctt atatggccaa cactggccat tactttatag
     9061 tttcgtccaa gatatttctc aattgtcttg gcttttgcag gtgattctac aatcactaaa
     9121 tatttatacg ccattactga atggactcct ttccattgtc gtgttcctac tattaaaaga
     9181 ggattcatac aaatcgtagc taatcatatc cattcagaaa acgtttgtca agaaaagcaa
     9241 atgaaaagta tggtgttatt ctttaaattc tgccaagata tcttcacccg aaaccgtgca
     9301 aactgcaccg tcttggatca aatgatgaca tccggtagaa tgttctgtta aaatatttcc
     9361 tggaacagaa aaaactgttc ttccaagatc caatgcttgc tgtgctgtta tcagagagcc
     9421 gctttttttc ttggcttcaa agacacatgt tcctttagac aaagctgcga taatccgatt
     9481 tctcattgga aaacaatgct ttttcgggcc ggattgagga ggatattcac tcaaaagaag
     9541 atgatcattt gcaatcacct cttgcaagtc acgattttca gcaggataga cttgctctat
     9601 ccctgttcca atgactgcga ttgtccgtcc accatgagag attgctgctt ggtgaccgta
     9661 tgtatctatc cctttcgcgc agccactgac gatcacatat ccagcttcta tcactggttt
     9721 tagtaaataa taaactgttt ttttggctaa agcagaagtg tgtcttccac caacaacgct
     9781 taacatgtta caagaaagta aagatagatc accattataa aacagtacaa ttggcggata
     9841 ggtcaagtga agcagctgag ggggatattt gggatcaaag caagtgataa acgactgttt
     9901 cgattggatt ttgccccagt tagcgtgaat ttcctgccaa cttttgcgaa acagctcccg
     9961 gtgtttggtt attttaccta tcttaatgat gtcttcaaat ccgatttctt tataaggata
    10021 ctgcatcatc cagttagcaa gtgcccattt tccttgacta ctgattcctt ttgtataagc
    10081 aagtttcaat aaattttctt ctatttgata catcataaaa ccgcctttcg tctctctttc
    10141 taataagata cgtaaaagcg gctgtttttt attgtaaata tttttttaca ggtgcaaaac
    10201 ttttacgatg gattggcgta attccttgtc gttcaagtcc ttctaagtgt tcctttgttc
    10261 cataacctgc attatgcgag aaaccatatc caggataaag cacgtcatat tctttcatca
    10321 attggtctcg atatacttta gcaatgatac ttgcagcagc aatcgacaca gatcgtgcat
    10381 cccctttgat tattttttcc tgctgaatag gcaaatccaa ggtcatcgca tcgatcaaca
    10441 aatagcttgg tacaatggac aatttttcta ctgcttggat catcgctaat ttacttgctt
    10501 cataaatatt cacatcatcg attgtctgct gatcaacgat cccgatcccg attgccaggg
    10561 ctttttcttg aacttgttga taaagaagtt ctcgcttttt ctctgataat tgtttggagt
    10621 cattcagccc taatatgatc tcatttttcg gcaagataac tgcagctgcc acgacaggtc
    10681 cagctaatgg tcctcttcct acttcatcaa tgccggcaat ccattggtga ccttgtgcgt
    10741 acccttgctt ttcaaagcaa aacatttctt catatttttc cttaattaat gcttcctttt
    10801 tcaatcgtcg ttcaaaagaa gccagtgctt gctgcacgcc tttgcgtgga tcatttctta
    10861 atgattctag tgtaggatca tctattctct gtatgctttt caatcgttct ttgatacttt
    10921 ggatggattc agtcatcact tttttgctcc atttcttcgc aacgatccag tgtgtatcgt
    10981 ccaagttttc cttgacgaat atcatgcacg actaattcgc tgccacgatc atagtcttct
    11041 ttatacccac gttttgctgt aatatccatc agtaattccg gtgccaattt ttcttcttgc
    11101 tcgattgata aaccataacg atcgctgatt cttcctgaat aatatcgggc aaaaaagtct
    11161 aatccgtaaa tcgctaaatc atctaaatgg acaagctggt ctttgatcgc gcctgtcagc
    11221 gctagttttt tgccgatttc ttcatcttca aatttgggcc ataaaatccc tggtgtgtct
    11281 aacaattcca actcattacc taaacgaagc cactgctgac cttttgttac gccaggctta
    11341 tttcctgtct gtgcaatttt cttgcctact agtcgattca tcaacgtaga tttcccaaca
    11401 tttggaatac ctaagaccat tgcccgaata gcgcgaggct tgatcccccg agcagcatct
    11461 cttgcccgtt tttctttcaa tgcttcttta gccgcaggta ctagaccttt aattccttta
    11521 ctttcttgtg ccgtgatagc taatgcaaga tggccttgtt tacgaaaata ttcttgccac
    11581 agctttgtct gttgtggatc tgccagatcc gctttattca ataaaacaag gcgaggtttt
    11641 tgctgaatga tctgatctag cattggatta cgagaagata aaggtaatcg tgcgtctacc
    11701 aattcaaata cgatgtccac atatttcatt ttttcagaaa cttctcgtcg ggcttttgcc
    11761 atatgtcccg gaaaccattg gatggtcatt gttctctcca ttcctttcat accggaatat
    11821 tccccggctt tacttgtttt tttctttttc aatataaatt cactttttta ttataaagaa
    11881 attccatcta ttagttaagt gaaagtgaac cagtcctcat tctatcacat cttcactttt
    11941 ttgatgatgc aaattt
//
LOCUS       DAXWLF010000079        11946 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXWLF010000079 DAXWLF010000000
VERSION     DAXWLF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.079"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            222..1097
                     /locus_tag="ACGE4U_001607"
     CDS             222..1097
                     /locus_tag="ACGE4U_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8393777.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            1111..1434
                     /locus_tag="ACGE4U_001608"
     CDS             1111..1434
                     /locus_tag="ACGE4U_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393778.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            1431..2183
                     /locus_tag="ACGE4U_001609"
     CDS             1431..2183
                     /locus_tag="ACGE4U_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFF8393779.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2173..4344
                     /locus_tag="ACGE4U_001610"
                     /pseudo
     CDS             2173..4344
                     /locus_tag="ACGE4U_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(4456..6627)
                     /gene="copB"
                     /locus_tag="ACGE4U_001611"
     CDS             complement(4456..6627)
                     /gene="copB"
                     /locus_tag="ACGE4U_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFF8393780.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(6647..8833)
                     /locus_tag="ACGE4U_001612"
     CDS             complement(6647..8833)
                     /locus_tag="ACGE4U_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8393781.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(8833..9042)
                     /gene="copZ"
                     /locus_tag="ACGE4U_001613"
     CDS             complement(8833..9042)
                     /gene="copZ"
                     /locus_tag="ACGE4U_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFF8393782.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(9055..9495)
                     /locus_tag="ACGE4U_001614"
     CDS             complement(9055..9495)
                     /locus_tag="ACGE4U_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFF8393783.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(9569..10067)
                     /locus_tag="ACGE4U_001615"
                     /pseudo
     CDS             complement(9569..10067)
                     /locus_tag="ACGE4U_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            complement(10352..11818)
                     /locus_tag="ACGE4U_001616"
     CDS             complement(10352..11818)
                     /locus_tag="ACGE4U_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8393784.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         3414 a         2518 c         2130 g         3884 t
ORIGIN      
        1 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
       61 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
      121 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
      181 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
      241 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
      301 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
      361 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
      421 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttggcag
      481 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
      541 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
      601 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
      661 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
      721 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
      781 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
      841 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
      901 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
      961 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     1021 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     1081 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     1141 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     1201 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     1261 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     1321 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     1381 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     1441 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     1501 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     1561 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     1621 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     1681 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     1741 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     1801 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     1861 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     1921 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     1981 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     2041 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gcttatcttg
     2101 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     2161 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     2221 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     2281 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     2341 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     2401 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     2461 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     2521 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     2581 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     2641 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     2701 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     2761 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaacc tctcgaggaa
     2821 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     2881 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     2941 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     3001 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     3061 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     3121 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     3181 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     3241 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     3301 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     3361 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     3421 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     3481 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc gaaagaacac
     3541 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     3601 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     3661 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     3721 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     3781 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     3841 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     3901 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     3961 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     4021 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     4081 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     4141 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     4201 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     4261 aaaacctatt tagaaataac gcgtataata agaaaaacgg tatacaaaaa aattctgaca
     4321 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     4381 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     4441 aacctttttt cattattatt tgattttcaa tgtcaaagca tttagagcta caactaccgt
     4501 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     4561 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     4621 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     4681 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     4741 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     4801 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     4861 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     4921 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     4981 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     5041 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     5101 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     5161 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     5221 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     5281 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     5341 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     5401 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     5461 tgatgtggag cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     5521 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     5581 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     5641 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     5701 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     5761 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     5821 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     5881 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     5941 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     6001 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     6061 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     6121 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     6181 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     6241 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     6301 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     6361 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     6421 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     6481 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     6541 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     6601 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     6661 gtcgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     6721 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     6781 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
     6841 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
     6901 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
     6961 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
     7021 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
     7081 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
     7141 tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca gccacggcaa
     7201 tccacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
     7261 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
     7321 gtgtacccgc aaaatagcgt actccattaa tcgtaccgct gatcccagca cctgggtggg
     7381 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
     7441 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
     7501 ttgaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
     7561 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
     7621 catttcgtgc accaacaccc gttcctacca taatagctgt tggggtcgct aaacctaatg
     7681 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
     7741 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
     7801 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
     7861 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg acttccatgt
     7921 gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga ttcttttcta
     7981 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
     8041 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
     8101 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
     8161 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
     8221 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
     8281 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
     8341 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
     8401 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
     8461 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
     8521 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
     8581 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca taaccgatgt
     8641 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
     8701 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
     8761 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
     8821 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
     8881 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
     8941 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
     9001 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
     9061 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
     9121 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tagcttcttc
     9181 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
     9241 cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga atttttttcc
     9301 tttttgttct gtccagatca cttctttttt taccaaacgt cccagcaaag ttttgatcgt
     9361 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
     9421 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
     9481 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt agcttatgaa
     9541 tacagattac gcttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
     9601 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
     9661 ctacttggat cagctgctgt atttttggtt cattgtttcc ttcaaaatgc cagataaaag
     9721 ataaaaattc taagtatttc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
     9781 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
     9841 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaacgtag
     9901 aagtataatt tccttcaacg atccatgacg gattagatac tataaacgat tgctgttctt
     9961 gcaaaaacca cttttttgcc tgaatgcgga aagccggtga tttctttgat cttccttgcg
    10021 agggacgatt ttcccgaccc actttgtcca atcacgatga tcttcattct tctccctcac
    10081 ttctctttgg aatttggttc gatcatacca cttataacgc cgcaaaaaaa caagggactg
    10141 tgacaaaagt cgtgtcaaag tcccttgttt cgaataaatg gtgttcaatc agctgacctt
    10201 cagcattagc tcaaaaaacg gcaaaatatg aggagctatt tttgtttggc tgttcttatt
    10261 acttgatgca ggacagcttt ttcacaacct ctgattcacg gtgttccaga agtagcttct
    10321 gtcacaacct cttgtaacaa gtatttctat tttaaggctg aaccactgtt tctggttcag
    10381 taatttcttt gtttttgtct cgacgagcaa ttgctggcaa gatcatacct aataagatca
    10441 agacaattgg tgtgatgata ttcgtgaata attggaacca ccatgcttta ggatcagcag
    10501 tataatctac ttttggcacc attcctagga tacatgcaaa cgcagtgaag gcaaagcacc
    10561 aagcaccaaa gataaaaccg atttttggat ttttaacaaa tttatattca gaattgaatt
    10621 tcttataagc tttattcaac atcatatatg ccaagaacac ccataggtaa cgcattggca
    10681 tgacaactga gttcaagttc gtcaaccatt ttactaattc attcatatct ttgataccaa
    10741 agatcggcaa caaaataatg attcctacta gtatacctgt cagaagataa ccattcacca
    10801 atgtaccttt acgattacgt ttacgtagcc agtttggtac aaattctgga tccgcatctg
    10861 ctaacaagat ttttagtggt gcatcaattg aaaaggctaa tgcggagatt tgtccaatca
    10921 tgtttgtcaa tgcgtaaatg atcatcaatg agttaccgat accatagtaa ttacctaaaa
    10981 tttgaaaagc actgtatgcc ccatttgcca tcaagtcttt agggatatta tcgctagcaa
    11041 acagcatacc cattgcgact gagcctaata cagcacaaat ccctaccatt gctgctaaga
    11101 agatcatccc tttaggaaat tcctttgcag gatttcttgt agagttgaca taaggggata
    11161 ttttttccgc ccctccaact gcaaatacta gcatcgaaac ggttgtgaaa taactgaaat
    11221 caaatgttgg aatatatgtt ttcacttgat tcatatgtgg tgtggcaaat tccatcgttg
    11281 gattgatgaa cggtgccgtt acagctaaaa gaataaataa gatcgacatg acgaacatcg
    11341 ctgttccggc cagtccgcca atcactttca aagtagaaag tccttttgtt gataaatata
    11401 aaaaggcaag aaatataata agtgaaataa ctgctaccat ttgtactgac atcgtattta
    11461 cgatatttcc atttccttgg actgcccaac caccagcaat cagaattgct tgaggctttt
    11521 gtgccaaata aggaatatgg acgacccagt aagtccaagc ggcatagtat gccagtcgtt
    11581 tattagatgt cgtattttcg acccatgaac tgacgcctcc tttactatcc ttgaaggctg
    11641 atcctaattg tccaacgatc aatgcgtatg gaataaaata aatggccaaa atcaatagcc
    11701 aagaggtgac aactgatact ccttgctgag catagttatt aactacattc cccatgcccc
    11761 aaaccatgtt aaatgcaatt aacgcaacag taaaccagcg taattgtttg ttgttcattc
    11821 gttctctcct ttatgataaa aatttgttat ccagatgtaa tcatattcaa tttttaacaa
    11881 acttacaaga cagtgttatt ttatgtgtgt attattttat ttaatacaca tcgttttttc
    11941 atattt
//
LOCUS       DAXWLF010000080        11945 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXWLF010000080 DAXWLF010000000
VERSION     DAXWLF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.080"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            17..259
                     /gene="yaaA"
                     /locus_tag="ACGE4U_001617"
     CDS             17..259
                     /gene="yaaA"
                     /locus_tag="ACGE4U_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFF8393785.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            246..1370
                     /gene="recF"
                     /locus_tag="ACGE4U_001618"
     CDS             246..1370
                     /gene="recF"
                     /locus_tag="ACGE4U_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFF8393786.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1367..3313
                     /gene="gyrB"
                     /locus_tag="ACGE4U_001619"
     CDS             1367..3313
                     /gene="gyrB"
                     /locus_tag="ACGE4U_001619"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFF8393787.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3333..5804
                     /gene="gyrA"
                     /locus_tag="ACGE4U_001620"
     CDS             3333..5804
                     /gene="gyrA"
                     /locus_tag="ACGE4U_001620"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFF8393788.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6040..6339
                     /gene="rpsF"
                     /locus_tag="ACGE4U_001621"
     CDS             6040..6339
                     /gene="rpsF"
                     /locus_tag="ACGE4U_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFF8393789.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6389..6901
                     /gene="ssb"
                     /locus_tag="ACGE4U_001622"
     CDS             6389..6901
                     /gene="ssb"
                     /locus_tag="ACGE4U_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8393790.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6927..7163
                     /gene="rpsR"
                     /locus_tag="ACGE4U_001623"
     CDS             6927..7163
                     /gene="rpsR"
                     /locus_tag="ACGE4U_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFF8393791.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7306..9279
                     /locus_tag="ACGE4U_001624"
     CDS             7306..9279
                     /locus_tag="ACGE4U_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFF8393792.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9285..9737
                     /gene="rplI"
                     /locus_tag="ACGE4U_001625"
     CDS             9285..9737
                     /gene="rplI"
                     /locus_tag="ACGE4U_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFF8393793.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10011..11378
                     /gene="dnaB"
                     /locus_tag="ACGE4U_001626"
     CDS             10011..11378
                     /gene="dnaB"
                     /locus_tag="ACGE4U_001626"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFF8393794.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         3988 a         2176 c         2647 g         3134 t
ORIGIN      
        1 aaatgaggga tgggcattga aacaacaagt cattctaaat acggaattca tgacgttggg
       61 ccaaatgttg aaagaagtca cagttatcgg tagtggcgga caagcaaaat ggtatttggc
      121 agaaaacact gtgttagtcg atggcgaacc agaaaatcgt cgtggacgca aactttatcc
      181 cggcatgatg gttgaagttc cagaagtagg aacttttttt atggtaaaaa aaggaaacga
      241 cgataatgag gctgaataag ctgtatttaa aaaattatcg aaattacgaa gagctgaaca
      301 tcgaattttc caagaacctc gtcatctttt taggagaaaa tgcgcaagga aaaacgaatc
      361 ttttggaaag tatctacgta ttagctatga cgcgaagtca tcggacaagt agtgagcagg
      421 aattgattgg ctggactgac gatcaggcga tgattcaagg agagatcaca aaaggttcat
      481 ccactcttcc attagaaatc ttattatcta aaaaaggccg aaaaaccaag atcaaccata
      541 tcgaacagaa aaagttaagt acttacgtgg gacaaatgaa tgtcatttta tttgcaccag
      601 aagatctttc tttagtaaaa ggcagcccgc agaccaggcg gaagttttta gatatggaat
      661 taggccagat caatccaatc tatctttatg atcttgtcca ataccaagca attttgaaac
      721 aaagaaacca gtacttgaag caattgaatg aaaaaaaaca aactgatttg ctttatttgg
      781 atattttgtc cgaacaacta gcagcgtttg gcagcaaagt attgaaagca agagcacagt
      841 tcgtcaagcg attggagttt tgggctaatt ctttgcatca gcaaatcacc catcaaaaag
      901 agcagctgga aatcgagtat ctcaccgcag tcgattcttt agaaacacat acgcaagagc
      961 aaatccaaga acaatttctc gcacttttga accaaaataa aaagaaagac ctttttcggg
     1021 gaacaacgac agttggtcct catcgtgacg atctttcctt ttttattaat cagaaaaatg
     1081 tgcagactta tggatcgcaa ggacagcaaa gaacaactgc actaagtgtc aaattagcag
     1141 aaatcgattt gattaaagaa gagacagggg aatatccaat ccttttgtta gatgatgtga
     1201 tgagcgagct ggatgataat cgtcaactcc atctgctaga aacaatcgaa ggaaaagttc
     1261 aaacgttctt aacaacaacg acacttgacc atgtaaaaaa taaaatgact gttgaacctg
     1321 agatttttta tgttcaacaa gggaaactaa aaggagaaga aactgaatga cagaagaaag
     1381 aagtttagta gaacgcgcta aagagtatga tgccagtcag attcaagtac ttgaaggact
     1441 tgaagccgtt cgtaaacgac ctggcatgta tatcggttcg actagttctg agggtctgca
     1501 tcaccttgtt tgggaaattg tcgataattc gatcgatgaa gtattagcag gttttgcaac
     1561 gaaaatccat gtgattattg aaaaagacaa cagtatcacc gttattgacg acggtcgggg
     1621 gatacctgta gatatccagg caaaaactgg acgtcctgca gttgaaacgg tctttactgt
     1681 tttacatgct ggaggaaaat tcggcggtgg tgggtacaaa gtttctggtg gacttcacgg
     1741 agtaggatct tctgttgtta acgcgctttc tacacagctt gatgtcaaag tatacaaaga
     1801 aggaaatgtg tactatcaag aatatcgtcg tggagctgta gtggacgact taaagattat
     1861 cgaacaaacg gatcgccatg gaacaacggt tcactttacg ccagaccctg aaatttttac
     1921 agagacaact gagtttgatt tttcaaaatt ggctacccgg atccgtgaac ttgctttctt
     1981 gaaccgcggg atgcgtatat cgattgaaga caaacgagaa gaagaaccag tcatcaatga
     2041 ataccattat gacggcggga ttaaaagtta tgtcgaatac ttgaatgcaa acaaaaccgt
     2101 gatttttcca gaacctgtat atttggaagg tgaacagcaa gatatcgcag ttgaagtctc
     2161 tatgcaatat acagacggct accattccaa tatcatgagt tttgccaaca atatccatac
     2221 gtatgaaggc ggaacacatg aatcaggatt caagactgct ttaactcgtg tgataaatga
     2281 ttatgcccga aaacaaaagc tgatgaaaga aaatgacgac aacttgaccg gtgaagacgt
     2341 gcgagaagga ttgacagctg ttatttctat caagcatcct gaccctcaat tcgaaggaca
     2401 aacaaaaaca aaattgggca attccgaagt acggacagtc actgatcggt tatttagcga
     2461 acatttcatg aaattcttgc tggaaaaccc aagtgtgggc cgtcaaattg ttgaaaaagg
     2521 attactagct tccagagctc gtctagcagc gaaacgtgcg cgtgaagtaa cgagacgtaa
     2581 aggtgcttta gagattagta atctgccagg aaaattagcg gactgctcta gcaacgatcc
     2641 tgaaaaatgc gaattgttca tcgtcgaagg agattcggcc ggcggatcag ctaagcaagg
     2701 tcgtaatcga gagtttcaag ccatcttgcc tattcgtggg aaaatcttga atgttgaaaa
     2761 agcaagcatg gacaagatct tggcaaatga agagatccgg tctttattta ctgctatggg
     2821 tacagggttt ggagaagatt ttgatgtatc gaaagctcgc taccacaaat tagttatcat
     2881 gactgatgcc gatgtcgacg gcgcgcatat ccgtacattg ctgctgactc ttttttatcg
     2941 ttacatgcgt cctattgttg aagctggata cgtctatatt gctcagccac ctttatacgg
     3001 ggtaaaacaa gggaagaata ttacgtatgt acaaccagga aaaaatgcag aagaagagtt
     3061 gaaacaagtc gttgcttcac ttcctgcctc accaaaacca agcgttcagc gttataaagg
     3121 acttggagaa atggatgatc atcaactatg ggaaacaact atggacccaa gcaatcggat
     3181 gatggcccgc gtcagtgtgg acgatgctat tgcagcagat cagatatttg aaatgttgat
     3241 gggggatcga gtagaacccc gtcgtgcatt catcgaggaa aatgcacact atgtgaagaa
     3301 cttagatatt taatcatgaa ggaggaaaaa gaatgagtga agaaatcaga gaaaacatcc
     3361 atgatgtcaa tctaaccagc gaaatgaaag actccttcat cgattatgcg atgagtgtta
     3421 ttgtcgcacg tgcacttcct gatgtcagag atggtctgaa acccgttcat cgccgtattt
     3481 tatacggcat gaacgaatta ggtgtaacac cggataaagc ccataaaaaa tctgccagaa
     3541 tcgtcggaga tgtcatgggt aaataccatc ctcatggaga ctatgcgatc tatgagtcaa
     3601 tggtacggat ggcacagcca ttcagctacc gttatatgct agtggatggt catggaaact
     3661 ttggttctgt tgacggtgac ggtgccgccg ctatgcgtta taccgaagca cggatgagca
     3721 aaatcgctac agaaatgctg cgtgatatca ataagaacac tgttgatttc caaagcaact
     3781 atgatgatac agaaaaagaa ccagttgtac tacctgctcg cttcccgaat cttttagtga
     3841 acggaacaac ggggatcgcc gtaggtatgg ccacgaatat cccacctcat aacttgtctg
     3901 aggtagtagc ggctatcgat ttgctgatgg ataatcctga tgtgacgacc aatgaattga
     3961 tggaagtatt acctggacca gattttccaa caggtggttt ggttatggga aaatcaggaa
     4021 tccgaagagc gtatgaaaca ggtaagggtt ctattaccgt acgtgcaaaa gttgagatta
     4081 ctgagatgcc aaatggtaaa gaacggatca tcgtgacaga attgccttat atggtcaata
     4141 aagcaaaatt gatcgaacgg atttctgaac tgcacagaga caagcgtatc gaagggatca
     4201 cagatttgcg ggacgaatct tcccgtgaag gtatgcggat cgtcattgac atccgtcgtg
     4261 acgccagcgc atctgttatc ttgaataact tatataaatt gacatccttg caaaattcat
     4321 ttggattcaa tatgttggcg atcgaaaaag gtgtgccgaa agttttgagc ttgaaacaaa
     4381 ttcttgaaaa ctatatcgaa catcagcgcg aagtcatcac aagacggaca gtctttgaaa
     4441 aagaaaaagc agaagcaagg gcacacattt tagaaggttt gcgtattgct ttggatcata
     4501 tcgatgaaat cattgcgatt atccgcgggt ctaaatcaga tgacgaagca aaagcgacga
     4561 tgatcgaacg gttcgattta tccgatcgcc agtctcaagc tattttagat atgcgtttac
     4621 gccgattgac cggtttggaa cgggaaaaaa tcgaaaatga ataccaagaa ctcttaaaac
     4681 tgattgaaga tttaactgac attctggcaa gaccagaacg tgttacagaa atcatcaaga
     4741 atgaattagc agacttgaat caacgatttg gtgataaacg acgcacagaa ttactagtcg
     4801 gtgaggtctt aagtctggaa gatgaagatt taatcgaaga agaagaaatc gttattacat
     4861 tgacaaacaa tggttacatc aaacgtttgg caaataatga attcagagct caacgaaggg
     4921 gtggacgtgg tgtccaagga atgggtatcc acaatgacga cttcgtcaaa aatctggttt
     4981 cttgttctac ccatgataca ttgttattct ttacgaataa cggaaaagtg tatcgggcaa
     5041 aaggatacga aattccagaa tacgggcgca cagccaaagg aataccaatt atcaatttac
     5101 taggtatcga ttcgagtgaa agcatccagg caatcattgc ggtgacggga gaagcagaag
     5161 aaggccacta tctcttcttt acgacgcgtc aaggtacagt aaaacgtact tctgtcactg
     5221 cttttgctaa catccgaagt aatggtctga tcgcaattgg tttgaaagaa gacgatgagc
     5281 tcgtcaatgt gctactgaca gatggtcaat ctaatatcat tattggaaca cataacggct
     5341 actctgtcac tttcaaagaa gaagcagtcc gcgatatggg acgtacagct tccggggtta
     5401 gaggaatacg cctgcgtgag gaagattatg tcgttggggc agccttaatg aaggaagatc
     5461 aagaagtatt agtgatcaca gaaaaaggtt acggaaaacg gacaaaagta tcggaatatc
     5521 cagtgaaagg ccgcggaggt aaagggatca aaacggcgaa catcactgaa aaaaatggtc
     5581 cactagctgg attgaccact gtttctggag atgaagacat catgctgatt acagataaag
     5641 gtgtcattat ccgtttcaac gtatcgactg tttctcaaac tggacgttca acattaggcg
     5701 tacgtttgat gaagatggaa gctgatacga aagttgtcac gatggcagca gtcgaaccag
     5761 aagctgcaga acaaacagtt gaagaacaag gatcagaaga atagccatca agactaattg
     5821 tgtaagctag caagaatgac cgattaaaaa atctggttat tcctgctggc ttttttgatt
     5881 agttgaattt ctcttaaaat attcgaaact gttcattctg tattgctttt gaccaaaata
     5941 gagtgtataa tcagagcttg tgagtagtct aattaattag ataactctcc ttgctcttag
     6001 gaacctaaga gccgaaagtc cacagggagg tgaatatcaa tggaaaacac gaaatatgaa
     6061 atcatgtaca ttattcgtcc aaacattgat gaagaagcaa aaactgcttt aatcgaacgt
     6121 ttcgatgcaa tcttgaaaga taacggagca gaagttatcg aatcaaaaga ttgggaaaaa
     6181 cgccgtttcg cttatgaaat gaacggttac cgtgaaggta tctatcacat cgttaaagta
     6241 tcttctccat catcagcaaa tgctgtcaat gaatttgacc gtcttgctaa aatcaacgac
     6301 gatatcattc gtcacatgat tgttaaagaa gaagcttaat tgttgtttca tgtgaaacaa
     6361 tagaaaacga tgagaggaga tgactatttt gattaataat gttgtattag ttggacgtct
     6421 gacgaaagat ccagatttgc gttacacagc aagtggtacg gcagtcgcaa catttacttt
     6481 ggctgtgaat cgtaatttta caaaccaaag cggcaatcgc gaggctgatt ttatcaactg
     6541 tgtgatttgg cgcaaatcag cagaaacttt agcaaactat gctcgtaaag gaacactttt
     6601 aggtgttact ggacgtatcc aaactcgttc ttatgataat cagcaaggac aacgtgtata
     6661 cgtgacagaa gtagtagctg acaacttcca attgctggaa agtcgttctg catctgaaaa
     6721 tcgccaacaa agcggcggat tccaaagttc tggtcaatca gctggcggat ttggcggaaa
     6781 caataactct aatcaaacat ctcaatcatc caacggtatg cctgatttcg accgtgatac
     6841 ttctgaccca ttcggctcaa gttcaacgat cgacatttca gacgatgatt taccattcta
     6901 aacgaatttg ataggaggga aatagaatgg cacaacaaag aagaggcgga cgtaaacgtc
     6961 gtaaagtaga ctacattgct gctaaccata ttgaatatat cgattataaa gatatcgaat
     7021 tactaaaaag atttatttca gaacgcggta aaatcttgcc acgtcgtgta actggtacag
     7081 gcgctaaaaa ccaacgtaaa attactgttg caatcaaacg cgctcgtatc atgggcttgc
     7141 tacctttcgt tagcgaagac taataaatcc taggaattga atgactgatc cttttgatca
     7201 gctattcaat tccttttttt ctgtaaaaat aacctctatt cgtttagtcg ctcgaatgac
     7261 gaaagttatg gtaaaatcaa attagtcata tatgggagga aaattatgga aaataaaaac
     7321 aatcatttat ccccgaaacc tctcttcctt atcctgttta tattaatcga agtattggta
     7381 gtcttaattt taccatttcg ttttgttagc gcttcggtca ttctattgat gaatttatgg
     7441 ggtgttcgct attggttgaa aatgaaacgt tggcttattt taaataaacg tgaaaaaatc
     7501 cagcaggctt cgactgtagc tgaaaccaat ctagcttatg tctcagaagt catgcctgta
     7561 ggagtgattg cttatcagcc atcaagccaa aaaatcgaat ggctcaatcc attagcattg
     7621 aaaatcaaag atcaaagtgg attaacagag gaagaattgc tagatacttt ttttactgca
     7681 aaagaaaaaa agaaaaacca agtctatcta gcagataaaa catttcagtt tgatatggat
     7741 acagtaaagg gtgttgttta ttttaatgat attacccaaa accagttgtt aaaactccat
     7801 cagacgaaca gtcagccggt gattggtatt ttatctattg ataactatgc agatgccatt
     7861 gataaattgg atgacaaaga gatttcttat ttgaactctt tcgtcactac actagtttct
     7921 gactggatga atgaatacca tgtctttttc aaaagaatca acgcagaacg gtttttcttc
     7981 gtagcacaga tgatcgatct gaaaaagatg atggataaaa aatttgattt gctggatcgc
     8041 tttcgagcag aagctgaaaa acagagctcg cctatcaccc ttagtatggg gattgcttat
     8101 ggctcaagaa ccctcgatga aatcggtgga gaagctcaga ataatttaga tgttgcttta
     8161 gtccgtggag gcgaccaagt tgtcgtgaaa gaggtaaaag aaggagcaaa acctgtttat
     8221 tatgggggga aatctgctaa gaatgtcaaa cgcacgcgtg ttcgttctcg agcgatgagt
     8281 accgctttgc gtcggatttt tgatgaaaca ggcgatgtgt ttatcatggg gcacaagttt
     8341 ccggatatgg atgctttagg ctctgctttt ggtgtcagtt gtctggcacg cttcaaccag
     8401 aaaaaagctt acatcgttat caacgagaac gagatcattc cagatgtaga acggtgtctt
     8461 gaagaaatcc ataagcatcc tgaattagaa atgcaactaa tctctccaga acgtgccata
     8521 gagctgaaaa aagaagagga cctcctcgtg atggtggatt accataaacc ttctctatcg
     8581 atctctcagg gcctatatga gcaatttgat aagattgtga tcattgatca ccatcgtcgt
     8641 ggggaagagt ttccaagtaa acctctgctg acttatatcg aatcttcagc ttcttcagct
     8701 tcagagctag tttctgagct gatccaatac caaagcagca aaacaaatcg cttagatcgt
     8761 tttgaggcca cattattgct tgcagggatg gttgtagata caaatagttt caatgtccgt
     8821 acaactgcac gaacttttga cgttgccagc tatttaaggt cttgtggagc agacgcatcc
     8881 ttagtacaat atttattgag ctcggattta gcgacatact tggagatcag caatttgatt
     8941 gctggaagcg actatgtgac ttctgatatt gttgtggcgg cagggacaga cgaaaaagag
     9001 tataatagtg ttatagcagc aaaaacagca gatacattgc tttcgatgat caatatcaat
     9061 gcagctttcg tcattacaaa gcggacagac ggcttgattg ggatcagtgc cagaagtacc
     9121 ggcacgatca acgtccaaat catcatggaa tccttaggtg gcggaggaca cttcacaaac
     9181 gcagctactc aactggaaaa tacgacagtt gaggaaacaa aacagaagct atttgctgca
     9241 atcaaagaaa atatgaatca aatatatgat aaggagtaat ccaaatgaaa gtaatttttt
     9301 tacaagatgt aaaaggaaaa ggtaaaaaag gggacatcaa agaagttcca gcaggttatg
     9361 cacaaaatta tctaatcaaa aataacttgg caaaagaagc aacgaaaagc agtatcagtc
     9421 agttagcagg acaaaagaaa gcacaagaaa aacacgaagc tgaaatcttg gaagaagcta
     9481 aaaagttaaa agaattcttg gaaaaagaag atactgtagt cgaattgaag gcgaaagcag
     9541 gggaagacag ccgtttgttt ggttcgatcc cttcaaaaca aattgctgaa gggttaaaca
     9601 aacaatacaa aatcaaatta gataaacgaa aaatcgaatt agaaaatccg atccgttcac
     9661 taggctacac aaacgtacct gttaagcttc atcatgaggt gacggctacg atcaaagttc
     9721 atgtcgtaca agaataacaa atcttgatag cgatgttcag caggtaagta taaccagaag
     9781 gaaaaaagac aaaccgaaat ttatctgaga aaacagctgc agcaatgaaa gcaaaagctg
     9841 caaatcgatt tcagattgat ttcggtttct tttttgggaa cagcaaaata gcataaagaa
     9901 aacgagtata ataaaagaga actatttttt tattacactt tcggtacgaa tgatgacttt
     9961 tctctcattt tcttgtaaaa tgaagcaatt gaagacaagg agcgatacgt atgaatgaag
    10021 tgtggcagga tcgaatccca ccgcaaaata ttgaagctga acaagcggtt ttaggctctg
    10081 tttttcttga cgcagaaacg atcattgatg ccatggagta catagagcca aaagactttt
    10141 atcgtcgtgg ccatcaaatt atttttgaga caatgatcga gttaaatgat cgtaatgaag
    10201 cgattgacgt ggttactgtc aaagaccggc tggaacaagc caatttgtta gaagatgccg
    10261 gtggtttaag ttatctctca gacttggcat tagcagttcc gacagcagca aatatcgtct
    10321 actatgcgaa aatcgttgaa gaaaaatcat tgctgcgtac attgatccag acagcaacag
    10381 gaatcgttac gaaaggattc gagcaaggag aagaagttca aacaatttta gatgatgccg
    10441 aacgcagtat cttggaggtt tcggaaaaaa gaaatcgaag cggcttttta tctattgctg
    10501 aagtactgaa ctccacgatt gaaaatatcg atcgactagc tcaaaacaat gaagaaatca
    10561 caggtctacc aactggctat caaggtctgg ataaaatgac agcaggtttc cagcccgaag
    10621 aactaatcat tcttgctgca cgtccagcgg taggtaaaac agcgtttgca ttgaatattg
    10681 ctcaaaatgt aggaacaaaa acagatcgtt ctgttgcgat atttagtttg gaaatggggg
    10741 cagaatctct tgtaaatcgg atgctttgtg cagaaggttc gattgaggcc agccatttac
    10801 gtacaggtca gctttcggaa gaagaatggt cgaatctgat cgttgcgatg gggagtcttt
    10861 ctcgagcaaa tatttttatc gatgatacac cagggatcaa aatctctgaa atccgtgcga
    10921 aatgtcggaa actagcgcaa gaaaaaggca atctaggctt gatattgatc gattatttgc
    10981 agttgattga aggtaacggt aaagagaacc gtcaacaaga agtatcagat atctctcgcc
    11041 aactgaaaaa actggcgaaa gagctgaaag tacctgtcat tgctctttca cagttatctc
    11101 gtggagtaga acagcgacaa gacaaacgac cagtgctaag tgatattcgt gaatccggat
    11161 caattgaaca agatgcggac atcgtagcct tcttgtatcg agatgattat tatgaaagag
    11221 aaggcggaga tgacgatgat ggaaacgagc caaaaaatga caacgtcatc gaagtcatca
    11281 tcgagaagaa ccgaagcggt gcacgaggaa cagttgaact actgtttatc aaagaataca
    11341 ataagttttc gtcgctttcg ccaagagaag aattttaaag aagaggttgt gagccttgag
    11401 caatcccgag agctctcgta agaagatgca aggagtaacc gcaggaacga ctagagtact
    11461 taatttaaac agatctccaa agggattgtg acaaaagtct tgtcacagtc cctttttccg
    11521 aataaacggt gttcaatcag ctgacccttg gtattagctc agcaaacgtc aaaatatgag
    11581 gaactatttt gacgtttgtt tctctaatac tcaggtcaaa acgctgatct tacgacctct
    11641 tttccttaaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata tcatcaaaca
    11701 tgagaagcag ttttttgaaa ttgctccttg tgactcagag ttaaacggca ggctcacaac
    11761 ctctttctta aacggtgttc aaaagcttgc atctgcggta atacgcccaa tcaaacgaaa
    11821 aatatgagaa gctatttttg tttggctgtt cttattactt gatgcaggac agctttttca
    11881 caacctctga ttcacggtgt tccaaaagta gcttcttcga aaaataagtc gaaatttcac
    11941 aaaaa
//
LOCUS       DAXWLF010000081        11813 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXWLF010000081 DAXWLF010000000
VERSION     DAXWLF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.081"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            5..2377
                     /locus_tag="ACGE4U_001627"
     CDS             5..2377
                     /locus_tag="ACGE4U_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFF8393795.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACGE4U_001628"
     CDS             2438..3898
                     /locus_tag="ACGE4U_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFF8393796.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="ACGE4U_001629"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="ACGE4U_001629"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8393797.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACGE4U_001630"
     CDS             6129..6278
                     /locus_tag="ACGE4U_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393798.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="ACGE4U_001631"
     CDS             6353..6946
                     /locus_tag="ACGE4U_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFF8393799.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="ACGE4U_001632"
     CDS             6959..7858
                     /locus_tag="ACGE4U_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFF8393800.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="ACGE4U_001633"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="ACGE4U_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8393801.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="ACGE4U_001634"
     CDS             8724..8984
                     /locus_tag="ACGE4U_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393802.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="ACGE4U_001635"
     CDS             9429..9635
                     /locus_tag="ACGE4U_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFF8393803.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9656..9886
                     /locus_tag="ACGE4U_001636"
     CDS             9656..9886
                     /locus_tag="ACGE4U_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393804.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="ACGE4U_001637"
     CDS             9902..10339
                     /locus_tag="ACGE4U_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393805.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="ACGE4U_001638"
     CDS             10332..11039
                     /locus_tag="ACGE4U_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393806.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11203..11403
                     /locus_tag="ACGE4U_001639"
     CDS             11203..11403
                     /locus_tag="ACGE4U_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393807.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11419..11598
                     /locus_tag="ACGE4U_001640"
     CDS             11419..11598
                     /locus_tag="ACGE4U_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393808.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT         4421 a         1965 c         2458 g         2969 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tag
//
LOCUS       DAXWLF010000082        11721 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXWLF010000082 DAXWLF010000000
VERSION     DAXWLF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.082"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..132)
                     /locus_tag="ACGE4U_001641"
     CDS             complement(<1..132)
                     /locus_tag="ACGE4U_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFF8393809.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..841
                     /locus_tag="ACGE4U_001642"
     CDS             542..841
                     /locus_tag="ACGE4U_001642"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8393810.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            914..2275
                     /locus_tag="ACGE4U_001643"
     CDS             914..2275
                     /locus_tag="ACGE4U_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFF8393811.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            2297..2557
                     /locus_tag="ACGE4U_001644"
     CDS             2297..2557
                     /locus_tag="ACGE4U_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393812.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2571..3323
                     /locus_tag="ACGE4U_001645"
     CDS             2571..3323
                     /locus_tag="ACGE4U_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFF8393813.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3417..4022)
                     /locus_tag="ACGE4U_001646"
     CDS             complement(3417..4022)
                     /locus_tag="ACGE4U_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8393814.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(4075..5049)
                     /locus_tag="ACGE4U_001647"
     CDS             complement(4075..5049)
                     /locus_tag="ACGE4U_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFF8393815.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5427..6146)
                     /locus_tag="ACGE4U_001648"
     CDS             complement(5427..6146)
                     /locus_tag="ACGE4U_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8393816.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(6273..7544)
                     /gene="celB"
                     /locus_tag="ACGE4U_001649"
     CDS             complement(6273..7544)
                     /gene="celB"
                     /locus_tag="ACGE4U_001649"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFF8393817.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7697..9094
                     /locus_tag="ACGE4U_001650"
     CDS             7697..9094
                     /locus_tag="ACGE4U_001650"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8393818.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            9175..9615
                     /locus_tag="ACGE4U_001651"
     CDS             9175..9615
                     /locus_tag="ACGE4U_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8393819.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9763..10089
                     /locus_tag="ACGE4U_001652"
     CDS             9763..10089
                     /locus_tag="ACGE4U_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8393820.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            10090..10839
                     /locus_tag="ACGE4U_001653"
     CDS             10090..10839
                     /locus_tag="ACGE4U_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFF8393821.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10919..11374)
                     /locus_tag="ACGE4U_001654"
     CDS             complement(10919..11374)
                     /locus_tag="ACGE4U_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8393822.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3745 a         2053 c         2394 g         3529 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaagaaaa
      541 aatgaaaaat cggttaattg tcgcatgtgg aagtggagta gcaacgagtc agacaatcgc
      601 tagtaagatc caaagtatgt tagaagatga cggcatcgca tttcctgtag aagcagttga
      661 ttacaaatcg attcaaaatg agcttctaac tgctggaatc tatgtgtatg tcgcacaacc
      721 tgatgaggaa gtacttgaac aagcaaaaga tttgggtatt gaagtatttc caggaattcc
      781 atttttaact ggaatgggtg tagaacctat ttatgacagt atcaaagagt tagtacagta
      841 aaggaggatt gttggataaa aaagttggtt catagtttta tttactatat ttagaaagaa
      901 agaaggttta tttatggaag ttttccaaac cattatcaat tatgttttga acttaggatc
      961 agccattttt gttcccctga ttattttgat acttggattg ttggcaggga tgaaattaaa
     1021 aaaagcattc atgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat
     1081 tggctatatg tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat
     1141 cagtttgcct gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag
     1201 ttatgctttt atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa
     1261 ttggacgaag acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta
     1321 tttagtttat tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt
     1381 tatattggaa ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat
     1441 tccattagta acagtgactc attttatgac atctgccgct gtcttgttgc tgcctttcaa
     1501 tatgatcatg gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa
     1561 gaaaattggg atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg
     1621 ctttgctgct gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc
     1681 attaagtttg tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga
     1741 cgctatgtca gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga
     1801 ctggccgata ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt
     1861 gtttattggt tatgccatta ttttgccagg aaaccaagta ttgccattag cagggattat
     1921 caactattcg attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct
     1981 gatcttggga atcatcacta tgcctattta tttgtacggg gccacttacc ttgcgccaat
     2041 actcagtgat ttagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat
     2101 cacatggtca tccatagaag ggccggagtt cagggtttta tttgcggagg catttaatag
     2161 gaatattctc gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa
     2221 atatatgata aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg
     2281 aaatggtggg aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct
     2341 ttgtggtaca cttctgctat ttctatatgg actctttact cagaatatca tcatcaatct
     2401 attggtcatt tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga
     2461 gtacgatgaa aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa
     2521 agaaatattg agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg
     2581 gacgtatgtt atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg
     2641 aaaggaaaaa cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag
     2701 ggaaagacta catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt
     2761 ctcctgcaca ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc
     2821 atttaactcg tgaaatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag
     2881 ctgtcctaca tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc
     2941 ctaatttgac agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta
     3001 attgctctga agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat
     3061 taccgcatat gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag
     3121 gaatcgaagc tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa
     3181 ttgcctacaa gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag
     3241 gtgagaaaat cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg
     3301 ttattgcagc tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc
     3361 tgatccagat cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat
     3421 ttagttaaga ggtggtctgt ccattccctc gcttgcttga tttcgtccat cgtcaactga
     3481 tgacctgtta cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc
     3541 aattctttaa aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg
     3601 gttttatcct ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac
     3661 aaccctttat ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca
     3721 tttgaatagc cgacaagcag ccagtcactt aacggtatca gttctttttc gctgatttct
     3781 ttgatttttt catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc
     3841 aatccattac gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa
     3901 agcaatgtac tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca
     3961 cctgtgccat gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc
     4021 attttgttcc ctcttttctg tttctataga tgcaaatgaa gtagcatgaa gagattattc
     4081 acgtttttta tttgcatcac ttgtatcgaa aggacggaca taagcttcaa tggcttcacg
     4141 acgttcttct aagaatggag gaagagatag tttttctcct gctgattctg ctggttcatc
     4201 ttcaaggaat ccaggaccat cagttgctaa ttcaaacagt acgtgatgag cggctaggaa
     4261 gtactcagat tggaaataga aacgatcgac gaaaccagaa tttggcaatt caatcttatt
     4321 gattacttga atccagtatt ccaatgcttc tttgtctgct acacgcaatg ctaagtgatg
     4381 gatatttcca aatccttcga ttgcttttgg caagtctgtc cgatgttcaa taatgatgct
     4441 tgcaccattt cccccttcac cgatttcaaa taagtagaaa tctcctgaat ggtctgtttc
     4501 tttgaatcct aagatttttt caagaatcaa gcggatatgc tctaattgac tgacacggac
     4561 aaagacaggt ccaagtcctg tgatcgcaaa ctctgtcggt acattgctgt ttttccaagg
     4621 aatgccagct gctacacctt gattgttttc gtcagagatc aattgataat actgattatc
     4681 gaaatcttca aattcgatat attttttgcc gaaacgttca ttgattgctg aatgagtaac
     4741 gtcatatttt tcaaaacgtt ccaaccaaaa gtctaatgct gcgtcacttg gcacacggaa
     4801 agaagcacga gagatcgtat ctgttccttt ttttcctttt ggctgatttg gaaaatcaaa
     4861 gaaagtcata tctgttcctg gcgcaccagt atcgtctgta aagtaaagat ggtaagtttc
     4921 gatgtcatct tggttaactg tttttttgat taagcgcatt cctaaaatat ctgtaaaaaa
     4981 gcggtagatt tttgtagcgc tagatgtcat tgctgtgacg tggtgaattc ctctgatttg
     5041 tgtatccatg ataaatttcc tcctgttaac ttacaaaaag taagtagttg atttagacat
     5101 agtatagagg gtgcatctct attttgcaat taatttgtct tctaaagtat caggaaggaa
     5161 tcaagaatgg aaaagaggag ggacggatca ataaaataaa aagacttacg actagctgta
     5221 tcgatttcaa aaaaactagc tttaaaaagc taactttaga atcattacgg gtggtcataa
     5281 gtctttttat gctgagttat tttttcattt ttgagctaat gcttaggata gaatagaatg
     5341 ttgatttcac gatctcttct tgttacttga aactgcttac cactgtcacg atctcttggt
     5401 aaacggcttt ttcacaacct ctttggttaa gttgaacgca catccagcac agaatagttc
     5461 cgaacatcaa atcggtttcg gctggaagaa tattcaatcg gctgtccatc tttgagatag
     5521 acgacttgct gaacttccag tacaggatcg ttttctgcac agttcaaata ttgctgatca
     5581 tattcatcac ttttatctgc agaaatagca cgataagccc ctgcaaaatg gacattcaat
     5641 tcttgatgaa gatgatcata gatagaggct aacaaatgtt ctttttttaa acccggcaca
     5701 agatggactg gcataaaggt atgctctaaa acatatggct cgccatctaa gatccttaac
     5761 cggtgaatat tgtagacagg ttgctcggca tcgatcatca atcgttcttg gatatattga
     5821 ttagggaatt caacatcaaa atgaatgatt tgactcttca aagaacgatg tctgcgtttc
     5881 atttggagac tcaaaccttc atactcgtta gcgggcgatg tgtctttttg cataaatgga
     5941 tgattcaaga ttttcgttcc tgatcctcgc tgcgaataga caagtccttc cattgctaag
     6001 atattgatgg cttttttgat tgtcatccgg ctcgcaccaa attcttttac caagtcgact
     6061 tgattaggca aaaaactatc gagaggatac tcttttgaac ggatacgttc tcttaaaata
     6121 tttgcgattt cttcgtattt tggcacgtta ttcacctctt ctagagtata acgaaagatt
     6181 atgcttccga caactcgaag tagcatctgg aacattgttt attcgaaaaa agaggtcatg
     6241 acaagttttt gtcacaaccc cttaatgatc tattaagcaa tatctaaatc tggttgttta
     6301 tctagtactt tcaggaatgg gtaccagata aaggcaacga tgacaaagtc gatgacttgc
     6361 agcataccac caagccatga atttgttgct aagacaccgc ttgcaatgat tggcacagtc
     6421 catggaacag agacgccagt tggtgcaggg acaagtccag cagccattac caaataattg
     6481 aacgtcgtta cgattagagg tgccacaacc caaggaataa ggattgttgc attcaatacg
     6541 atcggtagac cgaagatcgc tggttcattt acattgaaaa taccaggtgc taaagctaat
     6601 cgtccaacat cacgatactg tttcttttta agaacaaagg ccatcaataa aacgacagct
     6661 aaagtcattc cagagccacc aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg
     6721 atgtgtggta attcttttcc atgttgataa gcttctaagt tatcaagcat cagtgtattc
     6781 caaatcgggt ccattacaga gttgacgatg atttgtccgt gaagtccgaa gaaccataag
     6841 aattggacaa agaacaaagc aatcaatgtt gcaggtaaac tacttcccaa accagttaat
     6901 ggtttttgga tcacttcata aacgacgtca tgtaagttag cattgaaaat accagtcatc
     6961 acagcattga ttactaagaa aatagttagt gtcactacag cagggatcaa agcggcaaat
     7021 gattttgtta ctgcaggcgg aactccatca ggcattttga tctgccagcc gcgtttcgtg
     7081 attctgcagt agatttctcc agccaagaaa gcagcgatca ttccaatgaa cattcctttt
     7141 gcgccaagac gatcaagtga aagcacacca gtcacatctt caccgccagc ggttttcatt
     7201 gcaaaaggcg taagaatcaa gaagctggca aaagacacgg caccaccgaa aataccttca
     7261 acgtcataag attttgataa ataatagccg ataccaaaag taacaaaaac cgacatgatg
     7321 ctcattgttg cattttgccc attaccgaat aaattcgata aagttccctt tgttgcgtca
     7381 ctgaaaaaag gcaagttatt gataacgaca acgatcgaac cgaacatcgt taaagggaag
     7441 gaaagcatga aagcatcccg cagcactgta aggtaacgat tttgccctaa taaattagct
     7501 aagggcatga ttttctcaga aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa
     7561 tttatttgca atcgattaca tacaaagtat aacgccaaaa aatttaaatg tctagtcatt
     7621 ttcataaaaa gtattgattt attttttagg gttcgttatg attacggtat caagaaaaaa
     7681 gagaggaagt aaaaaaatga aaacctacac attcccagaa aatttttggt ggggttctgc
     7741 tgctagcgga ccgcaaacag aaggaagagt aagtgatgat ggtaaaggag aaaatatttg
     7801 ggatcattgg tatgaattag agcctgaaaa attcttcgat caagtaggac ctgaaaaaac
     7861 ttcgcaagtt tacacaaaat acaaagaaga tgttcagtta atgaaagaaa ctggccataa
     7921 cacatttcgt acatctatcc aatggagtca tttgattcca gaagggacag gtgaaatcaa
     7981 tcaaaaagct gtttcttttt atcgagattt ttttactgaa ttgctggata atggtattga
     8041 accttttgtg aacctttacc atttcgatat gccactacca ctgcaagaaa aaggcggatg
     8101 gttatcaaga gaaacggtcg acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt
     8161 tggagatctg gtcaaaaaat ggtttactca taatgaacca atcgtacctg tagaaggagg
     8221 ctacttgtat caattccact atccaaatca tatcaatatg aaagaagcgg tccaagtagg
     8281 ctatcatgag aatctagcaa gcgcaaaggt aatcaaagcc taccatgaag gcggacatga
     8341 tggtgagatc ggaatcatct tgaatctgac accaagctat ccgagagatg aaacgaaccc
     8401 agaagatgta aaagcggcga agatcgctga cgcattcttc aaccgatcat tccttgaccc
     8461 tgcagtgaaa ggagagtttc cacaagagtt gatcgaaatt gtcaaagaat tggctataat
     8521 gccagagatc catgaaggag acctagaact catccgtgaa aacacgattg acctgctagg
     8581 aatcaattac tatcagcctc gtcgaatcaa agcaaaagaa actccaatca atcacgaaca
     8641 tagccccatg ccagatgatt attttgataa ttacgacatg cccaataaaa aaatgaatcc
     8701 atatcgtgga tgggaaattt acgaaaaagg aatctatgat attttaacga atacaagaga
     8761 aaactatggt aatatcaaat gctatatttc tgaaaatggg atgggggtcg aaggagaaga
     8821 acgatttgtt aacagcgaag gaattattga agatgattac cggatcgagt ttgttacgga
     8881 tcatttgaaa tacgtccatc aagcaatcca agaaggaacc aattgtgtgg gttaccacat
     8941 gtggacatgt atggacaact ggtcttggac aaatgcttat aagaatcgct atggctttat
     9001 cgctgttgat ttggcacaag acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa
     9061 aacggtctct gaaaaaaacg gatttgatgc ctgatatttt ttgataaaac gttcacaaaa
     9121 tgtttgttct aacactgtaa tcgtgctaca ctaacagtga aggaggcgtt tacaatggta
     9181 gatacttatc gtaatatttt agtagcaatt gatggttctg aaaaggcaga aaaagcattt
     9241 cttgaggcaa tcacgattgc caaacgaaat caggcaacgt tgcacatcct gtatgtcaat
     9301 gaagtaacag gcaattactt tggtgatttt gcatttgtta caacaagcct tcaggaagaa
     9361 ttggatgagg ttgcggaaaa ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc
     9421 ctgacagata tcgagacata tgttctttac ggttatccaa agacactgat tgctaatttc
     9481 catgaatcga aagaaccaat cgatctgatt gtcatggggg cgacaggtct gaatgcggta
     9541 gaacgtgcac tagtaggttc cactacttcc tatgtagtca accacgcatc atgtaacgtc
     9601 atggttgtaa aataaataaa ttaagggaac agaaagcaaa aaagcataac gcttcttttg
     9661 ctttctgttc cttttatttt tttgtaaaaa attactttac ctgatgaagt aatcatgcta
     9721 ttctaattac aacgacagat ggagtaatat ggaggaaaga aaatgatttc aagtgatgga
     9781 atacgcggat acaatgatgc gattatttta tcgatcctta acaaaaacga ttcttatgga
     9841 tatgaaattt ctaaaacgat tcgtgagagg acaaatgatt cctatgtgat caaagagaca
     9901 acactttact ccgtttttgc ccgtttagaa aagaatcaac tgattgtttc gtatcctgga
     9961 gaagtgactc atggaaaaaa gcggacatat tataaaatca ctgaaatagg gcgtaatctt
    10021 ttagaagaaa aaaagcaaga gtggctgacg acgaaagacg tagtcgatca atttttggag
    10081 ggaaattaaa tggatacaat ccgtaattat ttagacagtc tttttatcgg agtacctcaa
    10141 agtaccgaga tagataagct gaaaactgat ttgttggcaa atatggaaga ccattatcat
    10201 gaattgatgg gagaaggaaa aaatgaacag gaggccatcg gtacagtaat ttcgacattt
    10261 ggttcaatcg atgaattatt agaagaacta gatgtcgaaa aaaaacatca agctgacgag
    10321 acggaaacaa acacagcttc tatttattta agcgaagcag aaaactactg gaaagaatac
    10381 agagcggcaa gtctccaagt ggcatcaggt gttctattca ttagcttatc ttttgcaagt
    10441 tttcttttct tttgttcagc aggatatgtg ttcatgggta tttcttgtct gattttcggt
    10501 atagcattag ctgtcggttt ttttattgct tctggtatga aaatcacacg tttaaatcac
    10561 tttttgcacc atcggaaaat acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa
    10621 gagtatcagc gaagttttgg cttctcactg attgcaggaa ttggattatg tattttttca
    10681 cttttcccac ttttggcttc tttaatgtgg tatatggatg gtagtatcgg tgcctctata
    10741 ttttttgtta cggtaggaac gggtgtcttc ttgatcattt atggcagtct agtccgtcat
    10801 tcttatcgtc aattcactca aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa
    10861 tattctattt atccggcgta gaaaaaaaga ctgtgacacg cttttgtcac agtctttttt
    10921 atatataata atttaaaaat tcatcatcta aatccgtaga attttctaga ttgcttaccc
    10981 atgtaaaatc gttatagaca gatttcagtt gacttctgcg ccattgtaca gggataaaaa
    11041 gacgcatgct atcttcattg aatccgacta ataatttctt tccgtcagta atgatgggac
    11101 gttgtaacaa agtaaaatgc gagacgatca gatcgacaag ctctgaggtc gtacattggt
    11161 cgatttggtc tttcaaattt ttataatcaa tcgagcgttt agctaataga tcgtcaaatc
    11221 cgttttctgt taatgacaat aaataaaata tctctttcct tgttaaaggc tgtttaggtg
    11281 tgaaaattcg ttctctgtaa ggcatttgat ggtctttcag ccagtttgcc atcagatggt
    11341 aactatgtgt gcgagaagaa tagatcgtaa gcataaaaaa actccttaaa tagtccatta
    11401 atttgttaga tctgttgaga taaataatag gtaaggagct gtgcataagc attgatcaaa
    11461 acaacccaaa catcattaga aataactttc taaagtctta aacttgcgac gagaaacaac
    11521 tagactaata gaaaaaatgt cttagccatt tggcttcctg tttgtttttt tgcttatgct
    11581 gcagctcctc gggaaagggt tctaacaatt tgaagaaggt tgatgattga tctagtttaa
    11641 attgtgtgaa atttgaaatc tggtgttttt actagatgaa tcacctaaca actatgatta
    11701 taacttatag aacttctgtt t
//
LOCUS       DAXWLF010000083        11720 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXWLF010000083 DAXWLF010000000
VERSION     DAXWLF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.083"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(57..881)
                     /locus_tag="ACGE4U_001655"
     CDS             complement(57..881)
                     /locus_tag="ACGE4U_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFF8393823.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(854..1534)
                     /locus_tag="ACGE4U_001656"
     CDS             complement(854..1534)
                     /locus_tag="ACGE4U_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8393824.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1557..2495)
                     /locus_tag="ACGE4U_001657"
     CDS             complement(1557..2495)
                     /locus_tag="ACGE4U_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFF8393825.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2740..3591)
                     /gene="ispE"
                     /locus_tag="ACGE4U_001658"
     CDS             complement(2740..3591)
                     /gene="ispE"
                     /locus_tag="ACGE4U_001658"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFF8393826.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3745..6318)
                     /gene="mprF"
                     /locus_tag="ACGE4U_001659"
     CDS             complement(3745..6318)
                     /gene="mprF"
                     /locus_tag="ACGE4U_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFF8393827.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6459..7265
                     /gene="proC"
                     /locus_tag="ACGE4U_001660"
     CDS             6459..7265
                     /gene="proC"
                     /locus_tag="ACGE4U_001660"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFF8393828.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7344..8900)
                     /gene="rny"
                     /locus_tag="ACGE4U_001661"
     CDS             complement(7344..8900)
                     /gene="rny"
                     /locus_tag="ACGE4U_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFF8393829.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9311..10360)
                     /gene="recA"
                     /locus_tag="ACGE4U_001662"
     CDS             complement(9311..10360)
                     /gene="recA"
                     /locus_tag="ACGE4U_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFF8393830.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10469..11710)
                     /locus_tag="ACGE4U_001663"
     CDS             complement(10469..11710)
                     /locus_tag="ACGE4U_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFF8393831.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
BASE COUNT         3372 a         2605 c         2030 g         3713 t
ORIGIN      
        1 taaaaagaag cctataaaaa atttttcgtt tttataaggc ttcttttctt tataatttag
       61 ttattttttc ttctgaaggc aaccaatatt cgtttgatga tatttacaat caggaacaaa
      121 gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat ctagataaaa cgagctagtt
      181 agtcctgaca gcatccctag aaatcctaca caaacactga tagcaatcac gacattgaag
      241 cttttgccta atcgcatacc gattgctgcg ggaagaacca tgattgcgga tatcaaaagt
      301 gcaccggcaa tcgggatcat cacagcaata gccacaccag taatcacatt gaacatcatc
      361 gacatcaaat gaacgggtag cccatccacg tgggcggtat cttcatcaaa agtcagaaca
      421 tacatcggcc gtttgaataa aaagaacaat ataccgatgg caacgaataa gattcctaaa
      481 aagatgactt gctcccatgt aatggtcacg atcgaaccaa acagatagga ttggatactt
      541 gttgctgaat tccctccgct caaattcatc aatactaacg ctagcgccaa accaccagcc
      601 attaaaatgg caatggagat ttctgaatag gttcggtaga tcgttcgcag atactccaat
      661 atcacagctg ctaatacaac gactagcatg gtcgtaagtg taggattaag gttcaaaaag
      721 aatcctaacg ctacccctgc taaagagaca tgagaaagcg tatcagccat cagcgattgt
      781 cttctgatca ctaaaaacac acctagcatc ggggcgattc cggcaatgaa gatagcggca
      841 agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca ttgagaatcc tcctttctca
      901 ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc atgatcatgg gtgatcatta
      961 gaactgattt cccatggaca tgcgcactat gtcggagaag acgataaaat tcatttctgg
     1021 attcttcatc catacctgtt gttggttcat caagaacaaa taagtcaggg tcagtagcaa
     1081 aaatacgcgc taaactgatt cgctgctttt gtccgccaga caattcacct attcgccgat
     1141 ttcgcatatc ccacatccct acagcttcta aagcacgctg tacatgttga tgatcttttt
     1201 cagagagacg cttgaaccag cggcctcttg gatatcttcc tgaacggact aattcgatca
     1261 ccgtactagg aaaacctgca ttgaaagatg caacttgttg aggaatataa ccgatactta
     1321 atttttttcc ttcttgattt ttctcagcaa tctttatcat tccttttgaa ggtttcagta
     1381 atcctaacga tgctttcacc aaggttgact tggctgctcc attttctcca gttaaaatga
     1441 caaattcacc cgcatctaca tgataagaga catcttctag tacaggttca tcatcatagt
     1501 aaaaggtcaa atcagatatt tcgatataac gcaccttaca acctccattc taaaacttaa
     1561 ttgatacttt cttttaaagc tttcagattt ttttccatga cagaaatata attttcgcct
     1621 gctttgattt gttcattagt caagctttct aaaggattca acacttctag cttcactcct
     1681 gtttcattcg acaatgtctc agctaccttc gaagaggcat tttcttcaaa ataaatcact
     1741 ttgatgttgt ttttcttcac aaattctttt agctcagcta agcgacttgg tgtgggttcc
     1801 tgatctggtg aaacgcccga aatcgctact tgttttaagt cgtattgatt cgctaaataa
     1861 gcaaaagcag catgctgagt gacaaatgtg cggtttgttg catcttttag ctcatcggag
     1921 tatttttgat ccagtgcttc aagctttttg atataagcat cggcattctt cgtaaaagtt
     1981 tcctttttat ctggatattt ttcgcctaat tcttcagcaa tcgttctgac ttcttttata
     2041 gctaataccg gatcgagcca aacatgtgga tcgtattcat gtgaatgacc atgcccttct
     2101 tctgattctt catgttcgtc tgcttcttgc gattctaaac ggtcgattcc tttagctgct
     2161 tcgatcatca aggtctgttt gctatccact gaatccttta acgaatccac aaaaaattcc
     2221 atgtctgaac tattatacac gaaaacatct gcatcggaaa tttctgctct ttcttttgca
     2281 cttggttcga aatcatgcgg ctccgttcca gcagggatca aaagtttcac gtttccttca
     2341 tcccctacga tctcttttgt aaaatcatac atcggataaa aagtcgtaac gatcgtcaaa
     2401 tcctcttttt tgtctgcttc cttgttcgta tttccacatg ctgcaaacag taaagcacct
     2461 atcattaccg tagctgtcaa aagatatttt ttcatttata tgctcctttc aatgaacaaa
     2521 tcggaatttt acgatttatt aacgtgaact aatgtatcaa aataaaaaaa cacagtcaat
     2581 agaaatccat caatttctga tttaagcatg tatattcttg attaaatcgt aaaatggatg
     2641 agttgctgct catatctagc catttcacta caataaaaaa ctgaagaata ccattttagg
     2701 attcctcagt ttttcatcac ctattttact tcttatattt tattgcaggg ttcgtactaa
     2761 ataaacttcc tcacaaaaac cttttaaccc attatatact ctctgcgctc ttgaataatg
     2821 ttgacacaag gcaaaaacgg ttggtccgct tccactcatc aatgcagcat ctgctccgta
     2881 cttgatcata cgctctttga tttgatggat gactggatgc cgctttcccg ttatatcttc
     2941 taaactgttt cccatttttt tgatcatctg ctgataatct tgctctcgga tggcttcagc
     3001 aagtccgtgg atgtcttgat gttggatggc ttgcgtatct acttctctaa acactgtacg
     3061 tgtagagaca ctcattcttg gtttgaccaa cacaacccag cactgtggca tagaaggtaa
     3121 tacttggatt tcttcccctt tgccaccgac aaatgccgta cttccgtaga cacagtaagg
     3181 aacatctgta ccgacttcca aaccgatttc tgccagttct tctaacgaac agcccagatt
     3241 ccataattgg ttgatccctc ttagagcagc tgcacagtct gtacttcctc cgcctaatcc
     3301 agctgctact ggaatattct tcttaacaaa gatcttgaca ccggttttga tattgtattt
     3361 attttttagc aataaagcag cttgataaac attgttccgt tcatctaccg gtaaaaatgc
     3421 tttatttgtt tctacaatga ttttgtcttc tgttatttct tcaaaaaata gatgatctgc
     3481 caagtctacg ctcgacatca ccatttccaa ctcatgatac ccatcttgcc gtctatataa
     3541 tacatccagt cctaaattta tcttggctgg cgctttttca atcaattcca tcctctcacc
     3601 cattcgaaaa gtctaagtcg caagtattct atcataggga aaaataaaag tatagcttat
     3661 gtctgcctag tggttgattt tgtcatcgtt tcaacagctt tcacagaatt ttctcttcat
     3721 aatccaaaca agttctagtg attcttataa ttctgtgagc acttcttctg ccaaactcgg
     3781 aacatttcct ttttcttttc ctatcagact gagtagctgc atggtagcaa atccgaaatt
     3841 acttcctttc ctataagcaa aataacgtcc ttcccaatga tcgacatatt ttaatttctt
     3901 ttcataagca gactggaagg aatagaaaaa attaccataa ttatatacga tatgcatgac
     3961 tttttcttga aagaaagaaa aatctgtttc tccaacatta gctaatggag ctaagcctag
     4021 attggcttcc gagtagccca ttttctcgta tacaataaac agattggcta acagatagtc
     4081 agctaattcc tcagattctt taggtaagac ccgcaacaaa tcatatcccg cctgcttctt
     4141 cgtcatcggc tgagcggtga tgaagccaac gactttttga tctttacgga caataccgac
     4201 accgctagat aatacatatg atttatcaaa tcgtcccaca gagaagtatt tttctcgtgc
     4261 accttgaagc catttttgtg aaacttcatt tacagcttga tgtaattctt ccggcagttg
     4321 atcgtaccaa gtaaaatggt atccttcttt catcaaacgt tgatattcaa gttgctgact
     4381 cagccgagac aagtcttctg ttgctagatc aacaatccct gcttcaccta ctttcgtgaa
     4441 gtgaaaaccc aattcatgaa gcatcactac gtagctttct gatatcttat aaaacgcaag
     4501 ctgatagcct aaacaatctg cttgtttcat aaactgattc gttgctgccc gcatcatttt
     4561 aggattgcca actggatctc ctaaaacaaa gcatttgttt cctttgatct gataaccaaa
     4621 aacgacttga tcttggtcgt cttcttggta ataaaaataa ctgtaacctg acaagcgaag
     4681 ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa cggtcaaaac ggtctttttc
     4741 gtactcactg ccaagcttcg tagtatcttc agctaaatat tgataaagcg ccactaaagc
     4801 tagcaatgag atgcccaaac caataagccc tgagaaccac acatcatctg aaggaaacaa
     4861 aataaaatga tttcctgtta ttcgaccgac taaaaagcgt ccgctgtgat agcctgcaat
     4921 tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca aagattatag ctccccaaga
     4981 ataaacgaat tttgttcgat aaaattcttt tctggataac cacacagcta acagaatcaa
     5041 gacatatacc aatatcaact gccaagataa cgtacgtgtg attgtattta aaataccaaa
     5101 agatagtaaa gcaatcgtag gcagaaatgc ctttttgact tttactgaaa gtgctctagc
     5161 aaggccgagt aataaaaagc caaccaatag attcactgtc tgatctaaaa aattgaatga
     5221 gaacggcaat aatacttgaa atagccgact gattgcagac agattagtga ttgttgatag
     5281 aagtaccatc atgatccccg caaaatagac agcacttacg agcacgatat gcgcaacctt
     5341 ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg attttgactc ctgtatgtgt
     5401 gatgaacaat agaagtcccg tcataaaagg tagaacataa taaaacaacc gataatataa
     5461 tagccaaact actgctacat cttgccgaac agacaattga ctcattccta agatcatcaa
     5521 aacgtcaaaa gtccccattc cacctgggac catcgtcagc attcctatca atgtagcaat
     5581 catgaacata ggatagatcg aaagcatcga aatatctatt ctcatcaact tgccgatgat
     5641 caaaaatacc gcaagggctc ccgtccattg tccaaaggat gccagtagca gaccaagcaa
     5701 gccttttgga taaaactcga agaacaatgt ctttctcttt agataagcaa aaaagaaaat
     5761 gactggtgct aaaaaactac cgccgaaaag ccagatccaa tagcttcgaa aaatactttc
     5821 tgactgaata aaaaagacat caataaatgt aagaaacgac catacagaca aaccagtcag
     5881 cataaatagt gcgacttttg atacagtggc taaaaccatt tttctatttg tctgctttcc
     5941 ataaaaacta gcccgcagac ttgctcctac cacaccacca aaaccggcga gattgttgat
     6001 cgtgtttgtt acccacgcag caagaagaaa ctcttttctt tccatttttg gttttccttg
     6061 tttttctaag actctgatcg tcacccaatc atacaacaac atcggagtga cacccagcag
     6121 tccagccaga atcatgagca ggatcgtcat gcggctttgt tgtcccatcc tttgccaaat
     6181 atcttgccaa ctcatgccat tagcaatatt tgcgacttga tttgccacaa agactaacac
     6241 ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc tttatccatt gcagcatcaa
     6301 gtatcgttta tttttcacat tttcccattc ctaacttgta tacttttgtg tcatacccca
     6361 atgttatctt atttatttta atttttccac tttagacctg ttctttgctt gcgatgatag
     6421 aaaaagaaag atatcctaat gctaaaggag aggataacat gaaagtagga tttatcggca
     6481 cagggaatat ggcacaagct attattgatg gtttattaaa aaaaggaaca gtctcgaaag
     6541 aagaggtgtt cgtttccagt ggacattttg aaaatgcaca aaaatttgct gctgaaactg
     6601 gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca atcagatatc gttcttttag
     6661 ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact aagagaatat agccagaaag
     6721 tcttattcat ttctattgct tcaggaaaat cattaacaga tttagaaaca gcgcttgaag
     6781 atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa tgtcagcgta ggagcaggcg
     6841 tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca gcttcagcaa gctacgatga
     6901 tttttgaagc aatcggcagc gtctatcctt tagcagaatc agatttcagt acattcattg
     6961 gccttgctgg cagctcaccc gcattcgttt acatgctgat cgatgcgatg ggacggactg
     7021 gtgtactgaa tggtttgtcc aaacagacag ccactgagat tgctgcaaaa gcaattatgg
     7081 gttcttgtca aaaattcctt tcaagtgata aaaatccatg ggaacttgta gaccaagttt
     7141 cttctcctgg cggtacaact gttgcaggtg tagttgcctt agaagaagca ggattcatcc
     7201 cagcaatcat caaaggaatc aatacgacga tcgagaaaga tcttgagatg ttgaataaaa
     7261 aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag aagagctgtg gcttttgccg
     7321 cggctcttct tttgtatttt ttattattta gcataatcag ttgcacgagt ttcccgaata
     7381 acgattactt tgatgtgtcc aggataatcc agttcttctt cgattcgttt acgaatatca
     7441 cgtactaaac gaacagcgtc taaatccgag atttcttctg gcttgaccat tacccgaact
     7501 tcgcgtccag cttgaactgc gtaacttgat tctacaccgg cgaagctgtt cgaaatattt
     7561 tccagacttc ttaaacgacg gatgtagttt tctaatgatt cacttcgcgc accaggtctt
     7621 gctgctgaga gcgcatctgc tgcagctaca aggacagaga taacagaagt agcttccaca
     7681 tctccatgat gagaagcgat cgcgttgatt actactgggt tttctttgta cttggcagct
     7741 aattccgcac cgatctcaac gtgagatcct tcaatctcat ggtcaagtgc tttcccaata
     7801 tcatggagca acccggcacg ttttgctaat tggatatctt cgccaagctc tgctgctaag
     7861 ataccagcta attttgcaac ctcgaccgaa tgcttcaaga cgttttgtcc ataactagtt
     7921 ctgaatcgca gacgtccgat gatcttgatt agatctggat gaagtgtatg agcaccgact
     7981 tcaaacgctg cctcttcccc gtattcgcga atacgttcat ccatttcttt acgagatttc
     8041 tcgaccattt cttcgatacg cgctggatgg attcgtccat cttgtatcag tttttccaag
     8101 gtcatccgtg cgatttctct tcggattgga tcgaatccgg aaagaacaac tgcttccggt
     8161 gtatcatcga tgattaaatc gataccagtc agcgtctcaa gcgttcgaat attacgacct
     8221 tctcgtccaa tgatacgacc tttcatttca tcattcggaa gggtaacgac tgaaacagtc
     8281 gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta atgataataa gtttttcgct
     8341 ttgcgatctg cttcttcttt ggcacgctgt tcagattctt ttaccattag agttaactca
     8401 tgattcagct cttcttcagt cgatttcatg atgacatctc gtgcgtcctc ttttgataaa
     8461 ccagcaattt tttctagttc tttttgttga tcttcaatca ttttttcaac gtctttttct
     8521 cgctcttcaa ttaattgctg ttttgaacta agtgcttctt ctttactttc aagtgagttc
     8581 tcgcgttttt ctaacgagtc atctttacga ttcagcgttt gttcgcgttg aatcaaacga
     8641 ttctcttgag attttaattc taacttactt tctctcagct cactttcaac ttcagaacgg
     8701 tatttctgat tttcttcctt agcttctaac aatgcttctt tttttagagt ttctgcttct
     8761 ttttttgcac tatcgatgat tccagcagct gatgattgtg caccagctat ttctttctcg
     8821 tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga caattaaacc gatgatagcg
     8881 aggagaatgt tgaataccat aaaatccacc tccatactat ctttttcatt tttatcatct
     8941 atacagcttc tatgataaat tattctaaac tcgtcaatat gcatcttcgc atatatgtgt
     9001 acaaaaaaca tgcacaactt tattctaatg tttgtacccc gtagtgtcaa ctttaaataa
     9061 acaaagaaac ggctttctac ttattcatta aacgatggtt tttaaaataa atgacactag
     9121 tttcatatag gtaaaccttt aaattgagaa acttttctat taaattattc tttgtagatg
     9181 aacagatatt tttattttat gtacccactc gtataattaa ataactcacg atttctttat
     9241 tcaataaaaa aacggcaggt caaggagagt acctactctt cagactagcc gtttttgttt
     9301 atcataattt ttattcttct actaaaggca attcttcttg tcctttttca tcttctggga
     9361 cagcgacttc ttcaccaata ccataagctg ctctaactag agcagaaact tctgccatca
     9421 tttcaggatg attagccata tagattttag cgttttcccg tccttgacca atccgatctt
     9481 ctttataaga ataccaagca ccgcttttat caacaatgtc tttttctact gccatgtcta
     9541 gaagttctcc ttcttgtgag atccctaaac catacatgac atctacttca gcgattttga
     9601 aaggaggtgc tactttgttt tttacgactt tgatctttgt acggttacca acaatatctg
     9661 taccttgttt caattgttct gcacgacgta cttccaaacg gatcgttgcg tagaatttca
     9721 atgcgcgtcc tccaggagtg atttctggat tcccaaacat cacgcctact ttttcacgga
     9781 tttggttgat gaaaatagcg attgtttttg ttttgttgat cgaaccagag agtttacgca
     9841 atgcttgaga catcaaacgt gcttgtaacc cgacatgtga gtcacccatt tcgccgtcga
     9901 tttctgctcg tggaactaaa gcagcaactg aatcgactac tacgatatct acagccccac
     9961 ttgatactaa agcatcagcg atctctagac cttgttctcc tgtgtcaggc tgtgaaagaa
    10021 gtagttcatc gatattcaca cctaattttt gtgcatattg cggatctaac gcatgctcag
    10081 catcaatgaa agcggccgtt ccgccatttt tttgtacttc tgcaatagcg tgtagtgcaa
    10141 ctgttgtttt acctgaactt tcaggtccat atacttcgat gatacgtcca cgggggtaac
    10201 cgccgacacc taaagcgaca tctaacgcaa gagagccact tggaattgta gagatttgtt
    10261 ggtcgatttt ttcgcccaat ttcataatag agcctttacc atagctcttt tcaatttttt
    10321 tcagtgcagc atctaaggct gctttacgat catctgccaa tacgataatc ctccttatat
    10381 ttacaaatca gagcaacatt tcattatcct tatcatatct tttttagtga aaaaaagcaa
    10441 gaaaaaaccg aacaaatatt cgcatttatc attttcttaa aagctgtttt ctcaaaagat
    10501 caaatccttg cataaccgca ttatatctga tagcagcacg atctctggca aagtgatatt
    10561 tttccgaaac cacgccgtct ttagatgaaa tagcgatcca tactgttcct gcagattgac
    10621 cttctaacgt gtccggtcca gccactccgg tgaaagctac actgtaatct gttcttgcta
    10681 tctcacgtgc ttgaatggcc atctgctcca cacattcttt gctgactgtt ccatattctg
    10741 ctaatagctc cggatcaatc tccagaaaac cagcttttgt ttggagcgag taggtaacga
    10801 atccgcctgg aaatacttca gatacaccag caatgtcgcc taatgcggct tggaaggcgc
    10861 cggctgtcaa actttccgct gctgttacag tctgcttgtt ttctttcagt agttccacta
    10921 cgacttttgc tagcgaatta tcatcgccgt atccataaaa atattcccct actcgttctt
    10981 ggattttttc ttctaatgct aaaagagctt gattccctgc ctgaacatca tttgttttca
    11041 ctgtcagtcg taatgtcact tcatttggtt tggcatatgg tgcgatcgtt ggattgatct
    11101 gtgtctcaat cagatctttt agctcagtga ccagtcgcga ttcaccaatc ccataaaagc
    11161 gcaaaacttt agaaattagt ttttcttcag aaggaaaacg ttcctctagt aaaggacgta
    11221 cttgttccac aaacattggt ttcaactcat taggcggacc gggaagaagg atatacgcat
    11281 gtgtatctgt ttgataaaaa gtccctaaag caagccctgt acgatttggt agaggaaccc
    11341 cgccttcgat aatctgggac tgttgcagat tgtttttggt catttttcga tgggttgttt
    11401 caaaatatgc caataatttt ttgtatcctt ccgtattttg tatgagagac ttaccgaggt
    11461 gagccgcagt tgcttctttt gtcaggtcat cttctgtcgg tcctaatccg ccacataaga
    11521 gtatgagttc acttcttgtt tcagctagag caatcaactc ttctaaacgc tgctgattat
    11581 ctcctacaac tgtttgataa taaacttcga tacctagatc agctagctgt tctgacaaga
    11641 aagtggcatt cgtattcacg acttgtccta gtaaaagttc tgttccgaca gctattattt
    11701 ctgctttcat tacttcccgc
//
LOCUS       DAXWLF010000084        11691 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXWLF010000084 DAXWLF010000000
VERSION     DAXWLF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.084"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            136..411
                     /gene="rpsP"
                     /locus_tag="ACGE4U_001664"
     CDS             136..411
                     /gene="rpsP"
                     /locus_tag="ACGE4U_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFF8393832.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            423..674
                     /locus_tag="ACGE4U_001665"
     CDS             423..674
                     /locus_tag="ACGE4U_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFF8393833.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            798..1589
                     /locus_tag="ACGE4U_001666"
     CDS             798..1589
                     /locus_tag="ACGE4U_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393834.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1759..2280
                     /gene="rimM"
                     /locus_tag="ACGE4U_001667"
     CDS             1759..2280
                     /gene="rimM"
                     /locus_tag="ACGE4U_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFF8393835.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2270..3031
                     /gene="trmD"
                     /locus_tag="ACGE4U_001668"
     CDS             2270..3031
                     /gene="trmD"
                     /locus_tag="ACGE4U_001668"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFF8393836.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3167..3514
                     /gene="rplS"
                     /locus_tag="ACGE4U_001669"
     CDS             3167..3514
                     /gene="rplS"
                     /locus_tag="ACGE4U_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFF8393837.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            3824..4066
                     /locus_tag="ACGE4U_001670"
     CDS             3824..4066
                     /locus_tag="ACGE4U_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFF8393838.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(4154..4300)
                     /locus_tag="ACGE4U_001671"
                     /pseudo
     CDS             complement(4154..4300)
                     /locus_tag="ACGE4U_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            4357..4671
                     /locus_tag="ACGE4U_001672"
                     /pseudo
     CDS             4357..4671
                     /locus_tag="ACGE4U_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            4700..5959
                     /locus_tag="ACGE4U_001673"
     CDS             4700..5959
                     /locus_tag="ACGE4U_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8393839.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            6012..6908
                     /locus_tag="ACGE4U_001674"
     CDS             6012..6908
                     /locus_tag="ACGE4U_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8393840.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPESAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            6919..7788
                     /locus_tag="ACGE4U_001675"
     CDS             6919..7788
                     /locus_tag="ACGE4U_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8393841.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            7798..8473
                     /locus_tag="ACGE4U_001676"
                     /pseudo
     CDS             7798..8473
                     /locus_tag="ACGE4U_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            8679..10343
                     /gene="alsS"
                     /locus_tag="ACGE4U_001677"
     CDS             8679..10343
                     /gene="alsS"
                     /locus_tag="ACGE4U_001677"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFF8393842.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            10356..11066
                     /gene="budA"
                     /locus_tag="ACGE4U_001678"
     CDS             10356..11066
                     /gene="budA"
                     /locus_tag="ACGE4U_001678"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFF8393843.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            11196..>11691
                     /locus_tag="ACGE4U_001679"
     CDS             11196..>11691
                     /locus_tag="ACGE4U_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFF8393844.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         3884 a         1917 c         2457 g         3433 t
ORIGIN      
        1 aaaagaagtc taaaatcttc tatttcctaa aaaaaatcga atttaatcaa gctgtaaaga
       61 aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg
      121 aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt
      181 ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa
      241 actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta
      301 gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca
      361 aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg
      421 atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg
      481 atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg
      541 ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca
      601 caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg
      661 ataaggaaaa gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt
      721 ttattaattg aagaaatgat tatttggcaa aacaagcaag aaagcgtatc cttataggag
      781 gagggagtga acagccaatg agaaagaaaa tgttatttat tccatttgct ctattgttaa
      841 gcgcttgtag ttcggttact caagaatcag acaatacaca tctatcaagc agtatatctg
      901 aaactgttac cagtccagaa aaaagtagta ccaaaacggc taccaccact gaacaaacaa
      961 tggctacctc aaataatgag aaaaagactg ctttagacca gttgaaagaa caacagccaa
     1021 atgttcccat gcctctagat gtaccagtct cttcaggcta tctcaatatc gcagcgacac
     1081 atacaaagca aggttattct attttatatt acagaacaga tcgtcccctc gggttgaatg
     1141 ctgacgaatt gaatcaagaa acgcctattg caacctatct atatcaatat gggtttgctt
     1201 ctagtcaaga aacaatccaa gttttgcagc cctttgagat cgacacaaat ggtcagcaag
     1261 tggacttagg ttctcgaatc acagggtatc agcaaggtgc agcaggttca agttttcttg
     1321 aatggcaaga aggaaactgg cgtattcgaa tccgtgggaa taatatagaa ggccaagatc
     1381 ccttactatt agctaaagaa atcgtagctt atttggaaga aaacagtcta ccagcacctg
     1441 aacaatttgg taaaattact gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa
     1501 gctggcaaga accgaaaaat gcctatacga tcactcatca agatcctatg tctgcgttga
     1561 agatggcagt ttctatgaag aggttgtgaa atcagcgcgc tgatctgagc ccatcattta
     1621 actccaagtc acaggaagta atcaaaaata gcacgccgca tttttgcata attttggctt
     1681 aataccttaa aagaaaacta gaattgcctc ttcacacatg ctaaaataaa acagacaaaa
     1741 aagaaatggg gatataagtt gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa
     1801 ggtatcaaag gcgaagtccg tgttatctca acaacggatt tcccagaaga acgttacaaa
     1861 aaaggaacgg tgttaacact attccaagac ggaaaagcac ctgtggaatt aacagtcaaa
     1921 agtcatcgca aacataaaaa ttttgactta ttgagttttg aaggacatcc ttctatcaat
     1981 gatgtggaaa aatatcggga tggcatatta aaagtgtcaa aagacaactc tgtagatcta
     2041 gctgaaaatg aattctatta ccatgagatc atcggtctgc atgtattaga agatggaact
     2101 gagttaggta aagtaaaaga aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa
     2161 cgtccgaaaa aagctgatat attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat
     2221 ttagaagctg gagtggtccg tgtagaaatt ccagaaggac tgattgacga tgaagattga
     2281 tgtattgact ttatttccaa gaatgtttga aggtcctatg ggggaatcca tcatcggtaa
     2341 ggctgtagat aaaggattat tagatatcaa tatctctaac ttccgcgatt attcagataa
     2401 caaacatcag acagtagatg attatccata cggcggtgga gcaggtatgc tgctcaaagt
     2461 ccagcccatc tatgataaca tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag
     2521 agtaattctt cttgacccag ctggtaaacg attcgatcag aaaatggcag aagaattttc
     2581 acaagaagaa cacttagtat tcatttgtgg tcattatgag ggatatgatg aacgtatccg
     2641 cacattagtg acagatgaag tttccttagg agattatgtc cttactgggg gagaattagg
     2701 tgcgatggtc atgatcgatg caacagttcg gcttttgcca gatgtgttag ggaatcagac
     2761 ttcagctcaa acggattctt attcaaccgg tttattggaa catccccaat atactcgccc
     2821 ggcagaatat aaagggatga aagtaccaga agtattgaca aatggaaatc ataaattgat
     2881 tgaagaatgg caattaaaag aatcactaag aaggacctat caacgtcggc ctgatctttt
     2941 ggaaacactt gaatggacac ctcaaatgac gaaattttta gaagaaatca agaaagaaga
     3001 acaagaaaaa acagcagatg aagccaagtg actttttcta aaatattctt tacagtaggt
     3061 tgataatatg ttagtatttt atggtgagtg caaaagcact taactattac gatattccgc
     3121 tatgagaaaa gctcataaga atatttggaa taaggagaag aaaaccatga atccattaat
     3181 cgaagaatta acaaaagaac aattacgttc tgacatccca gcgttccgcc ctggtgacac
     3241 tgtgcgtgtt cacgcaaaag ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg
     3301 tgttgttatc aaacgccgcg gtgctggaat cagcgaaact tatacagtac gtaaagtttc
     3361 taacggagtg ggcgttgaac gtacattccc attgcataca ccacgtgttg caaaaatcga
     3421 agtagttcgc tacggtaaag tacgtcgtgc aaaattgtac tacctacgtg cattacacgg
     3481 aaaagctgct cgtatcaaag aaatccgtcg ttaatcattg ttatcagact gagaaagaac
     3541 ccttggtatt caagggttct tttttcttag catgtaattt gaacaccctg atccagtttt
     3601 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3661 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3721 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3781 tacttatacg gagcaagaaa aaatgaaagt ttgagacaca ttcatgaatg atggaaaagt
     3841 aaaacaagta ccaagttcaa caaaaaagaa aaacatccta ttgaaagaag tcttgaaacg
     3901 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3961 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     4021 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     4081 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     4141 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     4201 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     4261 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     4321 acgaataaat accaaaacgt acctcctaaa attagattca aagaagatag caaagaggaa
     4381 aaagatagtt ctgcagcagc tatagcggca tgtggattgc tagaactttc caaacaactg
     4441 cctgtttcag atcctcgtca tttcgattac gaagagatag caatcaaaat cttggaagaa
     4501 ttacagaaaa actatacaac gaaacgagtt aacggatcaa acggtctgct tctccatgcc
     4561 gtttatgata aaaacagctt aaaaggtgta gatgaatgtg tgatctgggg cgattacttt
     4621 tatgtagaag gaatcactcg tctagctaaa acatggtact gttactggtg atgtataaga
     4681 gaagaggagg attcatataa tgaagcgatg gaaaaaaatg atgattggat caatagtttt
     4741 aggaatgtca ctcgtatttg gtgcttgtgg taatcaagaa gacagtagtg aagaatcagc
     4801 aaatgaaatt accgtttcga catggaatta tgaaacaaca cctgaattca aagcgctctt
     4861 tgaagcattc gaaaagaaaa cgggaatcaa agtgaaagca gtagatattg cttcagatga
     4921 ttatgatacg aaactcacca cgatgctttc ttctggagac acgacagatg tattgacgat
     4981 gaaaaatctt ttgtcgtatt caaattatgc tttacgagat cagttagtcg atcaaacaga
     5041 acgaattaga gaattagaca ctgatgctgc tgaagaaaca tatgaaatgt atgatatcga
     5101 tggaaaaaca tatgcattgc catatcgaac ggacttttgg gtactatatt acaataagaa
     5161 aatgtttgat gatgcaggaa tcgaatatcc aaaaaatcta acttgggacg aatatgaaga
     5221 tttagcaaaa aaacttagta aaaatgacgg acaagtttac ggagcatatc aacatatctg
     5281 gcgttcgaca atccaagcta ttgcagctgc tcaaaacgag gcgaatcttg ttgaaccaga
     5341 ttatcaatat atgactgatt attatgatcg agcattgagg atgcaaaaag aaggcgcgca
     5401 gatggatttt ggcacggcta aatcaacgaa agtaacctat cagtctcaat ttgaagaaca
     5461 aaaagcagca atgatgtaca tgggaacttg gtatatggct ggcatactcg caaacaaaga
     5521 agcaagtaag acagaagtag aatggggaat tacagctatt cctcagaaga aaaaaggaga
     5581 atcggtcacg acgtttggct caccaacagc atttgctgtc aataaaaact caaagaaaca
     5641 aaaagcggcg caagaattta ttgaatttgc agccagtgaa gaaggagcaa aagtattagc
     5701 aggagtagga gtagttcctt catatcgtac ggatgagatt gatcagcttt attttaatct
     5761 cgctggaatg ccaacggatg aaatttctaa aaaagctttt tctcctgatg aaatcaaact
     5821 tgaatttccg attgatacgt atgggcctgc aatcgataag attttacaag aagaatatga
     5881 cttagttttg gttggagatg aaacgccgga aaaaggaacg gtaaatatgg agaaacgtgt
     5941 agctgcggaa aaagaataat catgaaagga gagaagccaa gagataaaac actcttggtt
     6001 tcgtcatcga aatggagaat agtataaata agaaagctac aaatcatttg cgtagaaaac
     6061 gaacgtggat cgcttattcg tttatcgccc cgaatttcat tggttttttc gtttttacat
     6121 taataccaat cctgttttcg cttgtactag catttatgaa atgggattct ttttcaacgc
     6181 cagaatctgc tggactagat aactttcgta aaatggccaa cgatgagaca ttttggattt
     6241 ctttgaaaaa tactttttta tataccgctg ctgtagtgcc tggcacactt gtttgttcgt
     6301 tagggctagc tttactacta aatcagaaaa ttagagggat gaagcttttt cgtacgatat
     6361 ttttctttcc gtatgttact tccttagtag caattgctgt tgtttggaat atgttgtttc
     6421 atccaacagt gggacctatt aatatctttt taagacgatt tattgaacat cctccaggtt
     6481 ggacttcaag ttcaaattgg gcgctttggg caattgtaat tgtcagtatt tggcgaggga
     6541 tgggttatta catgattcta taccttgcag gactacaaag tatccaaaaa gaactatatg
     6601 aagcgtcttc gctagatgga gcaaaccgtt ggaaacaatt tttgaatgtg acattacctt
     6661 cgttgagacc aacaaccttc tttgtaacga tcatgcttgt catcaattgt ttcaaggtgt
     6721 ttgaccttgt gcaagtgatg acggatggcg gccctggtcg agcgacaaat gtgctggttt
     6781 accaaatcta caatgaagca ttcaaaaaat ttgattttgg ttatgcttca gctatttcac
     6841 ttgttttgtt cgtaattgtt ttgggcgtta cggttgttca attttcttgg aataagcgtc
     6901 gtacttagga ggtgtgatat ggaagagagt gttcgaaatg aaagaaaaaa agcaaaaatt
     6961 cgttcaaaaa acatgccctt tattcaatgg ttgggtatga ttctgctgat tgtattagct
     7021 ttattgacga tcattccttt tttatggatg gtttctgctt cattcaaagc aaataatgaa
     7081 gtgttaacga ttccgattca atggatccct aaaacttggc atttagagaa ttatctggtt
     7141 atttggtctc gtattccttt agtgacattt tttaagaata cagctttttt aagcgttgtg
     7201 atcacgttga ttcagttgtt tacttctagt tttgctgttt acgggttttc tgaactaaat
     7261 tttaaagggc gcgatatttt atttttagca tatatcggca cgattgctgt cccttggcag
     7321 tcttatatga tcccgcagtt cattatgatg agaaaagtgg gtttaacaga tacgttgtgg
     7381 tcgttggtgc tattacaagc gttcaatgct tttggtgtgt tcttgttgaa gcaatattac
     7441 agtagtatcc cagattcttt atgtgagagt gcgcggatcg atggcttatc agagtgggaa
     7501 atctatcgca agatcatttt acctcttaca aaaccggcga ttgccagttt gacgatcatc
     7561 acattcgtta atacatggaa cgattatatg gggccattca tttatttgtc ttctacggaa
     7621 aataaaacaa tccagttagg gttgaaaatg tttattgggc tatttgatgc agagtacgca
     7681 ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg cttttatatt tttagcgatg
     7741 caaaaatatt ttgtagaagg gatcgcttca tcaggaatga aggaatagga ggtgattatg
     7801 aaagagcagc gttttgacaa aaatgtttat atggaattct tttattggtt gtatcaatca
     7861 ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt atatagcagc tgcgttcctt
     7921 ttgtcgatag atggaaggaa tgtcttttcc tttatttttt ctttactttt tattggtcca
     7981 ggttgcatcg tgatgatagg tatgacagaa gaattagcaa aagaaaaaag tttttcacca
     8041 gtacgtttgt tcataaagac gtttcgccaa ttttggctaa agggattttt ttattggcta
     8101 tttgcttgga tagttagtgt gataatgatt tttgattgtt tcttttttat acgtttttct
     8161 tatggaaaat ggttgattcc gttgtttgtt ttacttgctt gttttacttg cttgtttatc
     8221 agtttccttt agcatcaact gctggtattt tcaggtgaga aatccagcgt cgaaaccaaa
     8281 tcaagttcta cggatagcgt tctactatac gttgaaaaaa tggtatgtga gtttactaga
     8341 ttttttacta ttgacaagtt tgtttctctt ctttttcgta aagccccaat ggtgtattct
     8401 actaggtcct tctatcgtat ttggtttgat ttacttcaac aatcgtaaat tgatgagaac
     8461 tatggatctg tagactttat aaattgaaac tttcaattaa gaatgacagt tttttatgta
     8521 aaaactgtca ttctttttca ttttttgaaa catatggcta aacaaatgaa ataaaacatg
     8581 ctatactttt gaatgaaaca ggtttagaaa ctgtatcact tatttgtgat tatttctaac
     8641 taataattaa aaataaagca taaaaaggac gatgttcaat ggctgaaaaa atgaaacaag
     8701 gttcgaaatc tgtagtagaa agtttgatta atcatcatgt tgattatgta ttcggtatac
     8761 caggtgcaaa aatcgatggt gtatttaatg aattagagga tcaaggtcct gaattaatcg
     8821 tgactcgtca cgaacaaaat gctgcattta tggcacaagc aatcggacgt attacaggag
     8881 agccaggagt agttatcgca acaagtggtc ctggtgctag taacttagct acaggtttgg
     8941 taacagcgac agcagaaggt gacccagttt tagcaattgc cggacaagta aaacgaagcg
     9001 atctattgaa gttgacccat caaagcatgg ataatgcagc actgttccag ccaatcacaa
     9061 aatacagtgc agaaatccaa gatccagaaa caatttcaga agtgattgcc aatgcttatc
     9121 gtatggctaa gagttcaaaa aaaggcgcga gctttatcag tatcccgcaa gatgtagtgg
     9181 atgcacctgt ccaaggtaat gtcatcaagc cattgagcga tcctaaatta ggttctgctt
     9241 ctgcagatga tatccgttac ctagctgaac gcattagaga agcaaaacta ccggtactat
     9301 tagtaggtat gcgaggatca agtgaaaaag aaacgttagc catcaggcaa ttagttgaaa
     9361 agacagcatt gcctgttgta gagacattcc aagcagctgg agtgatctca cgtgaattag
     9421 aagcgcattt ctttggtcgt gtgggtttat ttagaaacca accaggagat atgcttttga
     9481 aacgaagtga tctagttatt gcaattggct atgatccaat cgaatatgaa gcaagaaact
     9541 ggaatgcgga aaaagatgcg cggatcattg tgatcgatga agcgccagct gaaatcgatc
     9601 cttttatgca gcctgaaaga gaattgatcg gtgatatctc agccactctt gatttattga
     9661 caggttcatt agagcctcaa caagtgtcag aagatgcaaa agaatattta gcatctcttc
     9721 aagcaaaatt aacggagcgg gacattgttc aatcaaaagg agaagcaggt atcttgcatc
     9781 ctttagaagt tattaacacg cttcagtcaa aagtaacaga tgatatgact gttaccgtag
     9841 atgtaggtag tcattatatt tggatggcac gccactttag aagttatgaa cctcgtcatt
     9901 tgttattcag taatggtatg caaacgcttg gtgttgcatt gccatgggcg atttctgctg
     9961 cattggtgcg tccaaataca cagatcgtat cagtttccgg cgatggggga tttttattct
    10021 ctgcccagga tttagaaacg gcggtacgta aaaaattgaa tatcgttcat ttgatttgga
    10081 atgatggaca ctacaatatg gtagagttcc aagaaaaaat gaaatatcag cgggcgtcag
    10141 gtgtagactt tggtccagtt gattttgtga aatatgcaga agcttttggc gctaaaggaa
    10201 tccgagctac aagtgtagag gaattagaaa aagctttaga agaaggattt gcgacagaag
    10261 gacccgtaat tattgatatt ccgattgatt accgtgataa tgagaaattg ggagaaacaa
    10321 tcttaccaga tcaattctat taaggaagtg tcgacatgac tgaaaaaatc ttataccagc
    10381 acgggacatt aggagctttg atggccggct tgatggatgg aacagaaacg attgctcata
    10441 tattagaaaa aggaactctg ggattgggaa cattgcatgg gcttgatggt gaagtcatct
    10501 ttttagatgg cgtagcttat caaggaagat cagatggctc agttgcccaa ttagatggaa
    10561 gtgaacttac cccctatgca gcaattacag attttacgcc agatactagt ttttctgtct
    10621 caaaaactgc agatggagaa caattgaaaa aagacatatt agtaagagaa gccggggaaa
    10681 acctattttt ggcagtgaag ataactggat tattcaaaaa tatgcacatc cgtatcatgc
    10741 cgaagcaaga aaaaccttac agaagactag caaaaatttc agagtctcaa ccagaattcc
    10801 aacaaagcaa tgtccaagga acattagtcg ggttctttac gcctgaatta ttccaaggag
    10861 ttgctgcagc gggatttcac ctgcatttta tcgatgatac acatacattt ggcggacatg
    10921 taatggattt tgaagtggct gaaggaaaag ttgagattag ccgcatctcg tcccttgtac
    10981 agcattttcc agttgatgat ccgacttttg ttcacgaaac gattgattat gctgatttag
    11041 cagcagaaat ccaacaagca gaatagaaaa agacagcgag atgcgttcaa ggtgattaac
    11101 ctgacgtatc tcgttttttt gaggagaaaa cctagcgttt acaaaaaaac tgagctataa
    11161 tcttctccat agatcaaaaa ggagcggttt ttatcatgtg gacagaaaaa cagaaaaaag
    11221 atgcatacga aattttatta ttacagcaac aaggattatt agaagcagaa gataactggt
    11281 tagcaaattt agcaaattca tctgcattgt tgaatgagac tttgcctgag actgtctttg
    11341 caggatatta tttattcgat ggtaatgaat tgattttagg accgttccaa gggcgagttt
    11401 cttgcacacg tatcaagatg ggaaaaggag tttgtggaga atcagctgaa aaacaagaaa
    11461 cattgatcgt tgatgatgtc aaaacccatg caaattacat ttcctgcgat tcagccgcac
    11521 aatcagaaat cgtagtgcca atggtaaaag aaggtaattt aatcggtgtt ttagatatcg
    11581 attgcggagt aacgaaaggg tatgatgaaa tcgatcaagc atttctagaa aaatatgtag
    11641 atcttttatt agcaaatttt aatataaatg ctttttcatt tattaataac t
//
LOCUS       DAXWLF010000085        11662 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXWLF010000085 DAXWLF010000000
VERSION     DAXWLF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.085"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..817
                     /locus_tag="ACGE4U_001680"
     CDS             <1..817
                     /locus_tag="ACGE4U_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFF8393845.1"
                     /translation="VDKKIKKLASTQQLQSRKSKLLFLALVFEDQSYVIIDQSGHPVE
                     YSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIPVLIEEKRSGKELANCWVGLP
                     EERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWMLPNEIRKKNTADSLVLTKAL
                     RSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLLGSWQRATKRIREGKPVMIGI
                     LKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYHKVIPASWSNGTVSLP"
     gene            897..1871
                     /locus_tag="ACGE4U_001681"
     CDS             897..1871
                     /locus_tag="ACGE4U_001681"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFF8393846.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            2051..3196
                     /locus_tag="ACGE4U_001682"
     CDS             2051..3196
                     /locus_tag="ACGE4U_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFF8393847.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            3333..3677
                     /locus_tag="ACGE4U_001683"
     CDS             3333..3677
                     /locus_tag="ACGE4U_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFF8393848.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            3948..5234
                     /gene="efmA"
                     /locus_tag="ACGE4U_001684"
     CDS             3948..5234
                     /gene="efmA"
                     /locus_tag="ACGE4U_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFF8393849.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVSYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(5372..5971)
                     /locus_tag="ACGE4U_001685"
     CDS             complement(5372..5971)
                     /locus_tag="ACGE4U_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8393850.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            6375..7496
                     /gene="mnmA"
                     /locus_tag="ACGE4U_001686"
     CDS             6375..7496
                     /gene="mnmA"
                     /locus_tag="ACGE4U_001686"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFF8393851.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            7742..9163
                     /gene="arcD"
                     /locus_tag="ACGE4U_001687"
     CDS             7742..9163
                     /gene="arcD"
                     /locus_tag="ACGE4U_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFF8393852.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            9177..10295
                     /locus_tag="ACGE4U_001688"
     CDS             9177..10295
                     /locus_tag="ACGE4U_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFF8393853.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(10355..11647)
                     /locus_tag="ACGE4U_001689"
     CDS             complement(10355..11647)
                     /locus_tag="ACGE4U_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFF8393854.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
BASE COUNT         3667 a         2057 c         2558 g         3380 t
ORIGIN      
        1 agtagataaa aaaattaaaa aattagccag cactcagcag ctgcagtcaa gaaaaagtaa
       61 attgcttttt ttagctctgg tttttgaaga tcaaagttat gtgattatcg atcaatcagg
      121 acatccggta gaatattctc cagcaaaata tacttatcaa gagggtcttt cttgtaaaaa
      181 atggaagctg atcaatgaag agccaatcga gttatctaga tggatcaaca gaaaagaaga
      241 tattcccgtt ttgatcgaag aaaaaaggtc aggaaaggaa ctcgcaaatt gctgggtggg
      301 attgccagaa gaacgttttt tgaggtataa gaaatgggct accccctcag gctatctttg
      361 tggtacttac gcggcagcag ttctccttgc ctattatcaa gactatcgta aggggtggat
      421 gcttccaaat gagattcgca agaaaaatac ggctgattcg ttggttttga cgaaggcatt
      481 gcgtagccag atccaaccac taggacttcc tacgattcct tttcaggtta gtacagggat
      541 ttctagtttt ttaaagaaga atggcaatca tgagcgtgca cgagcaactc ttctaggttc
      601 atggcagcga gctacaaaac gtatacgtga aggcaaacct gtgatgattg gtattttgaa
      661 agtacttggc agtacatatg gcaatcattg ggtgacagcg tacgcttatt ttgaaactga
      721 aacaggagaa cgttattaca aagtacacga caactggggc gattatcata aagtcattcc
      781 tgccagttgg agtaacggaa cagtttcact tccttaagaa tttgctgaaa ttaacagaaa
      841 atcttggaaa aatatgttaa actatcgaag aatagagaag ttttggggag gactacatga
      901 gtaataagta tcaggatgac acgttgaaga tttttagttt gaatggcaac cgtccattag
      961 ctgaaaaaat cgcgaaagtg ttcggaacag aattaggtaa aagcgtcgtg aagcaattta
     1021 gtgatggcga gatttccatc aatatcgaag aaagtattcg aggggatcat gtgtatatcg
     1081 ttcaatcaac gaatgcacct gtcaatgatt actatatgga actactgatc atgatcgatg
     1141 caatgaagcg agcaagtgcg aaaacgatca atgtcgtatt gccttactat gggtatgcta
     1201 ggcaagaccg cacggcgaaa cctcatgaac cgatcactgc gaaattgatc gctaacttga
     1261 tcgaagaagc aggggcaact cgagtactga ctttagatct gcacacggtt caagtacaag
     1321 gattctttga tattcctgta gataatttat ttacgatgcc gttgtttgct cattattacc
     1381 gtcaattagg tctgacggga gatgacatcg tagttgtttc gccaaaaaac agcggtgttc
     1441 agcgtgcacg tagtctttca gaatatttga acagcacatt agccatcgta gatcatgcag
     1501 atgatgcttc aactgagggt ggttatgtga tcggtgatgt tcaaggaaag acatgtatca
     1561 tggtagatga tatcttgaac acaggagcaa cgcttgcacg agcagcaaat gtattaaaag
     1621 aaaacggcgc aaaagaagta tatgcctgtg cgtcacatgg attattgtcc gatcatgcga
     1681 aaagtatttt agacaatgca ccaatcaaag atatttgtat cactgattct gttttcacag
     1741 aagaaaatcg tcatccagaa aacttaacaa tcgttacatg ttcagaatta atgggtgaag
     1801 cggtcaaacg catccatgaa aatacaccga tgagtccgtt attccgtctg gaagaaaaag
     1861 aattcgaata aacaaaaaaa agagggaaag aagttgtgac agaagtgagc agcttcaagg
     1921 agctgcgaca agacttttgt cacagctcct tttttctatg cccatgaaac gtcctctaga
     1981 caaaaatgag ctcttctttg gtttgttcgc tattttatgc tagaatgtgc ctagcaataa
     2041 tttggaaggg atgaatgaag tgaaaactat ttatctggat catgcggcaa ctacccccat
     2101 gcatccacaa gtaattgaag cgatgacggc aatcatgcaa gacacgtttg gaaatccttc
     2161 aagcatacat ggatttggtc gccacgcgca tgaaaaattg gaaacagcaa gacaaatcat
     2221 tgcagacagt ctgcaagcaa aacctcacga gatcatcttt aacagtgggg gaacagaagg
     2281 ggataataca gctatcttag aaacagcgtt ctcgcgtcaa aaagaaggca gacatctaat
     2341 cactacagct atcgaacatc ctgctgttct taataccatg aaatatttag agacaaaggg
     2401 gtttgacgtt acatatctgc ctgtagatga aaaagggaat ctgtcgatcg aagaggtcaa
     2461 aaaagcgtta cgagaagata cgatcttggt ctctgtgatg tacgggaaca atgaaatcgg
     2521 aaatttgatg ccaatcaaag aaatcggtga acttttagcg gatcatccag cttatttcca
     2581 tacagatgcg gtacaagcgt acggaaacca aacgatccat ccagaagaat taggaatcga
     2641 tttattaagt atctctgctc ataaaatcaa tgggccaaaa ggcgtcggtt tcttatataa
     2701 acgagacgga gtaaatatcc ctgcattgct tttaggcggg gagcaggaag aaaagcggcg
     2761 tgcaggtaca gaaaatttag cagggatctg gggtatggca aaagcagttg aactgctgtc
     2821 acctgaagaa cgtgaagcac gtaacaacaa atataaagga ttccagcaat tgattcttga
     2881 tacattagaa gcaaacgaaa tcccttatca agtaaatggt gatttaacga ataaactccc
     2941 tcatgtgttg aatcttcgct taattggcga agcaaatgat ttgctactga tgaagcttga
     3001 tcttaatggg gtcgctattt ctactggttc agcttgtaca gcaggaaata tcgagccttc
     3061 acacgtatta gaagcaatgt atggagaaga tacaccgata ctaaaagaat ccatccgtat
     3121 cagctttggt ttgggaaata ccgaagacga catccagtat ttccttgatc aattagttcg
     3181 tgctgtcaaa aaataactag tgaagtcatt cttatatgaa cagatgtctg agagaacgcc
     3241 ttcatttttt agggaactta actgaaaaac ctagtaaaaa tcaggataaa cttttataat
     3301 aaagagaaac taattttatc gaggtgaaaa tcatggcatt tgaaacaaca gcaacggtac
     3361 tgggcgcacc aattagctat cgtttgcatc ctgatgcaaa aagatataca ttgcgcgata
     3421 atggatttac agaaacaaac ggaggcaatt atcaattgat ccgaccatta gatgccacgc
     3481 cgcaaagcaa agaaggattc aaattaaaaa tcactgtatc aaaagatatt caaacattga
     3541 aaatgtcgat cactacagca aatggattga aagcagtcaa tattttcaaa gatccaaaac
     3601 agaaaatgag ccaagataaa ttttattttc tgatggatgg gatgattagc cgtgggttat
     3661 tcgaaaaagt cgaatgatct gtggatataa aaagacaact agttttgaaa cggcctattg
     3721 ttagatgact aactttgtaa ggacgacgca aattagttgt cttttttatt tttaaattta
     3781 acacatgttg ttatttttaa tattttgctg atttattgtt tctaagtaaa gaaatttaaa
     3841 ttgaaacgat tgttatttag ggaaaaaggt attttttgaa aaaagaatcc cgtataatga
     3901 ttgttgtgca aacgctatac aaaatgaaaa agaaaagagg gatgtttatg gaaaatgaac
     3961 agtcggtcgt tttaacgaat tggaagagaa attatttgtt ctttttatcg ggacagtttt
     4021 tgtcaggaat cactagtatg gtcgtccaat atgcgatcat ctggtatttg acaagagaaa
     4081 ccggttcggc gacaattttg agttttgcta ctttactagg aatgattccc atggttttac
     4141 taagcccatt tgtaggaccg ctagtggatc gctgggataa aaaagctctt ttgattgtca
     4201 cggatattat tgtagcgatt ttcgcgctta ttttagcagt agtcggaacg atttcagaat
     4261 ccttcccgat ttggctagtt tttgtgtctt tgtttatgcg ttcggtcgca caaacattcc
     4321 aaatgccaac gatccagtcg attatgccga caatcgttcc atcttctcac ataacaagga
     4381 cgaatggaca gttaggaatg gtccaatcag cgaatttcat cattgcgcca gctctaggtg
     4441 cggctttgtt ttcagtcgtt cctgtcagtt accttatttt attagatgta ttgggggcag
     4501 tttttggtgt cggcttattg atttttgtga agatcccaaa agtttctcct gaaatattgg
     4561 aagttccgct tactattttt aaagatgcaa aatttgggct gcagcaattg atggataata
     4621 aagggttatg gtacataacg atcaatggtg catttgtgat gctgttgttc atgccagcaa
     4681 tcagtctgta tccattgatg acgctagatt actttggtgg ttccgttgga caggcgggag
     4741 cagtggaagt agtctacgca gtcggaatgc tcttaggcgg tgcactgatc agttttatag
     4801 gaacatggaa agaccgaatg aaacctatta ttatagccta catcattatg ggcctgacga
     4861 tcggtgcaag cggattggtt ccaaacgata gtcaagggtt cttgtacttt cttatcttga
     4921 atgcaggagc aggttgtgca acgccatatt tcaatacact gctgatggca atgatccagc
     4981 agagttatga atcgaatgtt ttaggtcgtg tcttagggaa cttcaactct ttgatgaacc
     5041 tggcaggtcc aattggatta ttatttgcag gaccattagc tgatcgttta ggggtagaaa
     5101 aaatgttctt gttttccgga atcgggattt tattatgcgg aatcgtttta tttttaacgt
     5161 cagctgcccg aaattatgat aaagaattac agaaaaaact ggtaaaagaa caccatgagc
     5221 aaaaagacga atagtatgga atggagttgt gacagaagtc ttgttacaac tccatttctg
     5281 atcctaatgt tcctgtggca ataagcctac tcaaatgaaa aatatgagta attatcttta
     5341 tttgtttaat acttgatata gggcagtttt ttcacgacct atcgtacttc ctctttgctt
     5401 catagatttc tttgatttgt tgctcagata acggagtgcc aaatggtagt ttttccttta
     5461 agagttctct ttccggtgcg tctacgccta cattgacatc gttctcgata ggtacgctgt
     5521 aatgatggat atgtccatga agattgcgga tagtgtcgtt aggcccgagc aacatcggat
     5581 aatgagtcaa gtaatattgt tgatggtcga atttaacgat ggcacctaca tcataaaaat
     5641 aaaatttggg acttccgtca gtaagcctag gatcattttt ttctaaataa cggaaaaaag
     5701 cgcgactgtc atggttccct ttgatcaagt ggattgttcc ttgcagttga gtgaccagtt
     5761 ccaaaatttc tgcatttgct ttttcatatt gcggatgcat agcaaggtcg cctagatgat
     5821 aaacatggtc ggtttctttt attactgcat tccagctagt gatcaactgt tggttcattt
     5881 catccacatt tgcaaagagc cgcggagcaa agtcgttttt tcctaataag cgttcatgga
     5941 aaaagtgcat atcactgata aagtaattca tgtatctttt gcctcccttt tttcttagag
     6001 taaacaaaat cacttatctg agcaactcat aacatctaaa aagaaaaggt tgtaaatgag
     6061 acgtgcagct ttaagcgata agaagcaatt ttgaaaaata gtttttcaca atttataaaa
     6121 ttattcctta ttgccgaaag gctggccctt tttatgagta tgccaatttt ttatatcgaa
     6181 tgctcaaaag atgagtgatt ttttggttga ctatcatgtt tgtgtactga ataggtccag
     6241 tagaacagta tttttacgac ttgataatta agggatgaaa ttttcagaaa tatgacgtat
     6301 aatgtaaaat actgaaaagt tgcgaccttc aactcgtaga tgtttcagaa tctaattgaa
     6361 atgatggtga tatcatgaca gacaacagca aaacacgcgt tgtcgtaggg atgagcggcg
     6421 gtgtcgactc atctgtaaca gcacttttgt taaaggaaca aggatacgac gtgatcggaa
     6481 tattcatgaa aaattgggat gacacggacg aaaacggtgt atgcacggcc acggaagatt
     6541 ataaggatgt agcgaaagta gctgctcaaa tcgggattcc ttattactct gtgaattttg
     6601 aaaaagaata ttgggatcgt gtatttgaat atttcctagc cgaataccgc gcaggacgta
     6661 cacctaaccc agacgtgatg tgcaacaaag aaatcaaatt caaagcattc ttggattatg
     6721 caatggatct tggcgccgat tatgtagcta ctggacatta tgcgcaagtc actcgcgatg
     6781 aaaacggcgt ggtccacatg cttcgtggag tagacaataa taaagatcaa acttacttcc
     6841 ttagccaatt atcacaagaa cagctttcaa aaacgatgtt ccctctaggc ggcatggaaa
     6901 aatcagaagt acgggcaatt gctgaacgcg caggccttgc gactgctaag aaaaaagatt
     6961 caacgggaat ctgctttatt ggtgaaaaga acttcaaaca atttttgagc aactatctgc
     7021 cagcaaaaaa aggaaatatg gtcactcttg atggtgaagt caaaggacaa catgctggct
     7081 tgatgtatta tacgattggt caacgtcaag gactagggat cggtggcgga ggagattcgc
     7141 aagaaccctg gtttgttgta ggaaaagatc tagcgacaaa cacattatat gtggggcaag
     7201 ggttccatca tcctgcattg tatgccacaa gcttggatgc aagtgaaatc cactttacga
     7261 cgaatgaacc aatgccaaaa gaattcaagt gtacagcgaa attccgttat cgtcagcaag
     7321 acgttcctgt gacagttcgc ttattggatg ataatcgtgc agaagtcgta ttcgacgaac
     7381 ctgtacgtgc aatcactcct ggacaagcag ttgtatttta tgatggaatg gaatgtctgg
     7441 gtggtggttt gatcgaccac gcatatcaag aaacaaaagt tttacaatac gtttaagcga
     7501 tattaaatga atcaaaacag ctatggctaa caaaagaaac accttttctt ttggatagca
     7561 tagctgtttt tttgcaataa aattaatttt tattcatttt atacttgaaa tacttgtgta
     7621 aaaagttcat aatatccaat aatatgaata aagttgcttt ttgttaagca agagcagttg
     7681 caaaatagaa gggaatcttt tgcaagtgat ttgttcaatt ttataagtga gggtgatgga
     7741 aatggaaaag aaatcgacgg gtattagcaa atggggactg gttgctctgg ttgtcagttc
     7801 gtcaatcgga agcggtgtgt ttgggatcac aagtgatctg gctagttctg cagctccagg
     7861 gccagcgatc ctttcatgga taatcgttgg gattggtatt ttagctttgg tattatcgct
     7921 gaatcattta ggagaaaagc gtccagatct agatggtggt attttcggtt acgcggaagc
     7981 ttcatttggt aaattaggtg gcttcatcag tggttggggt tactggttgt ctgcgtggct
     8041 gggaaatgtt gcttttgcaa cgatgctgat gagcgccatg ggagaatttt tcccgatatt
     8101 caaaggtgga caaaatgttc cttctatttt atttgcaagt atttttattt ggggtctgac
     8161 tttattagta aacaatggag ttgaaggagc gagcgtcatc aatacaatcg tgacgatctg
     8221 taaattagtg ccattattcc tatttttaat atttgtattt atcgcattta aagtgaatat
     8281 attcactgct gatttttggg gtaacgtatc tgataatcta gtgaatggaa cagggcagca
     8341 aggaacgatc tggttgcaga ttaaaggttg tttgatggtt atgatgtggg tctttgtagg
     8401 gatcgaaggc gcaagtgttt tagccaaccg cgcgaagaaa cgttcagaag cccagcaagc
     8461 atcgatcatt ggtctgcttt gtttattgtt tatctatatc cttgcttcga tcttgccata
     8521 cggcgtctta tctcaagaag agttagcaac tatttcacaa cctgctatgg caaatatttt
     8581 aaaaggaatc gtgggaaatt ggggagcagc gctgatcaat atcggcttga ttatttcgat
     8641 cattggttct tggttgtctt ggacgatgct cccagcagaa acaacgatgt tgatggctcg
     8701 agacggtctg ttgccgaaag gttggggaaa aacgaataaa aaaaatgccc caacttattc
     8761 gttagttgtt actgcaatct tgactaatct attcttattg actttcttag ttactgatta
     8821 tgcctatcaa tttgcctatt ctttatgtac agcggcaatt ttgatttgtt atctacttgt
     8881 cgggatctat caagtccagt tttctttaaa agaaggaatc aaaaaacaag tagtgatcgg
     8941 tgggattgct gtagtttttg aattgatggg catcatttta gcaggatttt cttatgtcct
     9001 tttatgtagt attgcctatc ttcctggttt ttgcttctac tggatcgctt gtcgtgaaac
     9061 caaacatcaa attacgggaa aagaaaaagt aatgattggg ctgatttgtg tggccgcact
     9121 gctttcaatc gggttgcttg ctgtaggctg gatcaagata taagggggaa agaaacatga
     9181 aaatcgcaaa tttcggtgta gaagaatggc taaataaatg ggaaaagaaa gcggtatatg
     9241 atattgcaca aagctcgatc gaagcattga cattagacga actcgttggc ttagatggga
     9301 ctagcgtaag taaatttttc gaaaaacgca aaaacgaacc attggactat ggctggatcg
     9361 aaggatcaga tgttttcaaa caagaagtcg cttctcttta tcaaaccgtc gatccggaaa
     9421 acatcttaca aacgaatgga gcgactggtg ctaatctttt agctctatac gcgttagtgg
     9481 aagcaggaga ccatgttgtt tcgatgcttc ctacttatca gcaattatat gacatcccca
     9541 aatctttagg tgccgctgtt gactttgttc atttgaagga agaagaagat tggcagtttg
     9601 atttagaaca gctagaagcc ttagtcaaac ctgaaacaaa aatcatctgt ttgaatagtg
     9661 caaacaatcc tacaggcaca ttgctagatc ggaaaacttt agaaaaaatc gcagaaattg
     9721 ctcgtacagt agatgcatat gtattgatag atgaagtata tgcgccttta acggataaag
     9781 gtgaattctt gtcgatcgta gatgtctatg aaaaaggtat agcaaccaat tccctatcca
     9841 aaacctactc cattccaggt attcgtattg gctggacagc tactggacct gagttggcag
     9901 aagtttttcg aaagtatcga gactacacga tgatttgcgg cggagtcctg tcagatgatc
     9961 ttgctgtaca tgcattgaag aataaagaaa aaatattggc gcgaaacaaa aagatcatta
    10021 cagagaacct tgtgatttta aaacaatggg tagccaatga accgaaagtt gaacttgtcg
    10081 ctcccaatta cgtctctact tctttcatta aattagccat cgaagaagat gaccagacat
    10141 tttgtatcaa tttattagaa gaaacaggag tactattagt accaggaagt gcctttgatt
    10201 taccgaagca tgcccgacta ggttactgtt gtaaaaaaga aaccttggaa aagggattat
    10261 tcctcttatc tgagttcttg aaaaaacagg cctaaacaaa aacactgcaa ggacagtctg
    10321 tgacaactgt tcaagcagtg tttttcattt acgcttattg tctatgagaa ggagagtgcc
    10381 ctgaagctat ggagactcct gaagcttttt cttctgcagt ttcttgctga tggatctctt
    10441 gtgtattgtg tcgtacaagc aagaagttga ccaaaacaag aagagaagtc gaaagaaaga
    10501 cgccacgata accaaatgca ctggaaacac tggagccgat cataggccct actacgttac
    10561 cagttgcttg gaaagattgg ttataactga agatccgtcc agcagcttga tgaggcgaat
    10621 attttgtgat caatgcttgg acagcaggga gcagacaagc atcagagata ccaatcaaaa
    10681 aacgtaaggc agctaattgc caaacattcg tgacgaatgc cattgggata taaaccagaa
    10741 tcgcaaaacc caaaccaatc gcaagtattc tttcactccc aatcttgtca cctaagcgac
    10801 caaatcttgg agcggctatc aaagtggcaa tcccaggtat cgaagcaatg attccgctga
    10861 tcaacgtaac attcccatgt ccatgcagca attggcgaat gtacagactg atgattggac
    10921 taatggaatt gttggatgct tggatgatca tcgttgtgac gaacataccg atgacgacgt
    10981 gtggatattt caagtctttg aaaatctgtt tggcagaaac catgttttct tttttcactg
    11041 ggacaaattt ttcatggacg aaaaacagac tcaacaagaa gacgaaaaat aagatgatcc
    11101 ctgtaatgaa aaaggtaggg cgatagccaa acgctgaagc agagatcccg ccaagaagcg
    11161 gtccaagtaa tgttcctgta acagaaccag ttgctaaggt tccaagaact tgaccacttt
    11221 tttcttttgg cgtcccagtt gcgacaaggg cagtggcatt actgatataa ccagaaaaaa
    11281 tcccttgaag cagacgcaat gcgactaatt gatacacact agtcacgcaa cccatcaaag
    11341 aaatgacgat tgccattcct aatgaagcac gcaacagcat caattttctt cctttttggt
    11401 cggctaattt tccccaccaa ggagagataa gcgtagtcac caagaaagta gatgaaaaag
    11461 tcaatccgct ccaaaagttc aattgtgacg tactataatt acctaacgta tctatgtaaa
    11521 gtgacataaa aggcatgact aaactaaatc cgataccagc cataaatgtt ccaaaccata
    11581 agacaaacaa gttttgttcc caagtttctc ttttcctaaa taatttttcc tttattcttg
    11641 cagccaatta gactccttct tt
//
LOCUS       DAXWLF010000086        11609 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXWLF010000086 DAXWLF010000000
VERSION     DAXWLF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.086"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(44..841)
                     /locus_tag="ACGE4U_001690"
     CDS             complement(44..841)
                     /locus_tag="ACGE4U_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFF8393855.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(838..2022)
                     /locus_tag="ACGE4U_001691"
     CDS             complement(838..2022)
                     /locus_tag="ACGE4U_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFF8393856.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2047..3294)
                     /locus_tag="ACGE4U_001692"
     CDS             complement(2047..3294)
                     /locus_tag="ACGE4U_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFF8393857.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3509..5254
                     /gene="dnaX"
                     /locus_tag="ACGE4U_001693"
     CDS             3509..5254
                     /gene="dnaX"
                     /locus_tag="ACGE4U_001693"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFF8393858.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5274..5588
                     /locus_tag="ACGE4U_001694"
     CDS             5274..5588
                     /locus_tag="ACGE4U_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFF8393859.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5613..6209
                     /gene="recR"
                     /locus_tag="ACGE4U_001695"
     CDS             5613..6209
                     /gene="recR"
                     /locus_tag="ACGE4U_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFF8393860.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6273..6917
                     /gene="tmk"
                     /locus_tag="ACGE4U_001696"
     CDS             6273..6917
                     /gene="tmk"
                     /locus_tag="ACGE4U_001696"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFF8393861.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            6932..7261
                     /locus_tag="ACGE4U_001697"
     CDS             6932..7261
                     /locus_tag="ACGE4U_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFF8393862.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7275..8213
                     /gene="holB"
                     /locus_tag="ACGE4U_001698"
     CDS             7275..8213
                     /gene="holB"
                     /locus_tag="ACGE4U_001698"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFF8393863.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8249..9073
                     /locus_tag="ACGE4U_001699"
     CDS             8249..9073
                     /locus_tag="ACGE4U_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFF8393864.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9066..9413
                     /locus_tag="ACGE4U_001700"
     CDS             9066..9413
                     /locus_tag="ACGE4U_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFF8393865.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9482..10354
                     /gene="rsmI"
                     /locus_tag="ACGE4U_001701"
     CDS             9482..10354
                     /gene="rsmI"
                     /locus_tag="ACGE4U_001701"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFF8393866.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10463..11584
                     /gene="dinB"
                     /locus_tag="ACGE4U_001702"
     CDS             10463..11584
                     /gene="dinB"
                     /locus_tag="ACGE4U_001702"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFF8393867.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
BASE COUNT         3714 a         2174 c         2512 g         3209 t
ORIGIN      
        1 ttttgtcaca gccctttttt tgcgaacatt catttagcaa tttttagaaa cttctgccgc
       61 caccgccaaa agtcccgcca ccgctagaat gggtagtact tccgccaccg ccgcttccag
      121 gaggtgggct tttggggatt cttcttgtcg tgacaaaaga atttgtcaga cgatcggttt
      181 tttcagttaa tttgatactg gatttttctc ggaatggata tttgtagatg cctgatttca
      241 attgataacg cgaaacagtc acaaaataaa agacaagact aaggataaga gccaaaacaa
      301 cagcaatgat gatttctact gtcgtcaaaa ccttatagcg tgtaattttg ccagtttctt
      361 cgtctacacg ataatgtcct cctggaactc cgtcttccac atatgttgct gatttagaca
      421 ggtaagcttt tgcagcattg taataggcag catttgtcat ctgatcataa acatcatcta
      481 agatcgaatc gattctgcta tcagtcaaat aatcgatcat attaccagaa gtcgaaatgt
      541 agatttcacg ctgtcccata tctaatagca atactgagcc attttgatca tttccgactg
      601 cgtctcttaa gtaattatca gcaaagtctc ttggttcttc tgaattagat gtagtcgtta
      661 cgatcaagac tcgtcctttg attttttcat tcagcttttc tgcctgttgt tccaaagatt
      721 ggatttgatc tgttgtgaat aacccagcct catcatttac cgtagctgta tctgcatgag
      781 tagccaccgg ccagccaaca cttactagca aaagaaagaa taaaaaaaat tttttcatca
      841 tatcaagtaa cctccgatca gaaagaggat cgctagccca gcgaaaatcc caagagcggt
      901 cagtcccaat tttttctggc tgatcggcag gacaccgctg acctttcctg tttgtccatt
      961 cattgcgtag tagtagactt ttttacttga gtcattgctt cgataagtaa caagccatac
     1021 cggcagtaat acgtaattat ttacttcgtt tgtgattgaa gcagaagttc gcacaccggt
     1081 caatgttgta tatccatttg cggtatcttt taacagcttc tctgaatagt cttttaattc
     1141 agattgaatc tgtgatttga ttgcctcata ttcgatatct cttttttctg cttggaaccc
     1201 ggctaaatac tggcttttaa aatcaattgc tttatctaac gggaaaggtt gcacgccttc
     1261 gaccatcttt tgttgcgtat tttttgataa ggcatttttt actagttctt taaaagaaag
     1321 cttgcctgct cttgaaacag caaattgctt cgtttctgtg tattcgatgt cacctatccg
     1381 ccatatgcgg ataactgtac cattcgcctg catggcacca tctacttcag catctgtagc
     1441 ccaataaggg aaatacacac ctgtcaactt atcgatttga tccttattga aaaaagcttt
     1501 gggaacaaac cattttttct tggtccaagc gagaaatctg ttgattgctt cttctttctc
     1561 gacagcaaaa ggaagtactt tatttggcaa gaattctccg cttaaacgtc ccgatagtac
     1621 gacgggatta tggcagtagt aacaataagt cgcagcagtt gtcgcatcag tcacgatctc
     1681 tgcaccacag ttgggacaaa gaaaaagatc tatcgctgcg gtattatctg cagattcgtc
     1741 cgctgtatct gaaaaaccgc ctgcctcgtc aaccgctttg cgctcggctt cattcatatc
     1801 ggcaagctgt tcttgggctg taaaagtaaa atcttcggta ggtgaaggtt cttctttgct
     1861 ttcttcgcca gccatacgcg cttctttttg cttttgctca tattggctga cttcttcttc
     1921 tgtatagata ttcaggcagt attcgcaatg aaatttttgg tctttgggat caaaagtcaa
     1981 cggaccgccg caattaggac atttatgtgt aagaacatcc atcagctgca cacctttcta
     2041 aataaattaa ttaacgggga ttcctttaaa cggtttacca cagtttggac agaatttagg
     2101 gatgccgttg gaaagatcca cgatctcatt gcattcactg catcgcattt gtagcttagg
     2161 ctgaacgttt tctacaggtt tggcagctcc gcagttggaa cagaattttc cggaattttc
     2221 tgtcccgcaa actggacatg tccaagtatc cgcatttcct tgtgcagttt gttgattttg
     2281 tttttctgct tgttgtttga tttgttcttg gttattttga gctgcttgag aaagataaga
     2341 accatttgca ttcattccca tacctacacc cataaagccg gtcattgctc ctgcttcatt
     2401 ttttccggct gcttccatac cacgtgcaat ggaaccttgc acgtatcctt cacgtacgct
     2461 aggatcacct agcatcgccc cttcgttacg catattgatc agtttcacag aatcatcggt
     2521 gtaagaaatg ctggctacag ccacagaaac aatctccatc ccacgtaatt cacgccaaga
     2581 atcatccaat gcagtatcca tgtatttgct taattccaaa ctcttagatg gtacataaga
     2641 aatccgttga ccatctgcag acatctgatt gattgctgat tgtagcgctg ttaggaattc
     2701 agctaaatac tgctcattga tatcattgat ttccacttgt gttttgtttt tagggattgc
     2761 gtttgtataa aataaaatcg ggtcaacgac atgaatagaa taagtgccat gtgcccgtaa
     2821 gaataattct gcgttataga aattgtcaaa gtagttcaac ggcgcgcttg taccgaattt
     2881 gattcccttt atttcttgga gattgatata aaaaacttgt tgtttctgcg gtgtgacgcc
     2941 gccaaatttg aatcggtcaa aagtttcagc aattgcttct ttcagtgttc cattgaacat
     3001 ggaaggagct gcgtcatttt taatggtgta ataaccttcc tcagcggtat aatcgatgat
     3061 ttttccacca tcgaccaaaa gcatcatcat gtttggatag acatggacga ctgttccgtc
     3121 tgttaaaaca tcttctgttc ctttacgatt ggagccacgt ttgtcatctt tgcggacttt
     3181 gacacctttt gtcatgaccg ttgtatcact catattgtct ggttcgatga cttctagcca
     3241 ttgatctgcc agaccgccgc caaccatact tgttgctgct ttgataagac ccataaaaaa
     3301 ttcctcctct gattcagcta gtgccaaaca attattgttt atttattata ccataggaag
     3361 atgtgaaact tctcggacta gagacggaat tgaaatggaa aattgcgtat tgtttaaata
     3421 cctgaaaaag ccgagaagga tagtagtttt tttacctaat atcacgtata ataagagata
     3481 gtgaattttt taaaaaaggg ggatccttat ggcatatcaa gcactttatc gagtctggcg
     3541 ttcgcagcgt ttcgaagaca tcgttggaca aaaagctgtg acacagacac taaaaaatgc
     3601 tattgtttcc cacaaaacct cacatgccta cttgtttaca ggacccagag gaaccggaaa
     3661 gacgagtgca gcaaagattt tcgcaaaagc aatcaactgt ccgaacagta aagacggtga
     3721 gccatgcaac gaatgcgaaa tgtgtcgatc aattactgct ggtacgcaag aagatgtgat
     3781 cgaaatcgat gctgccagca ataacggagt cgaagaaatc cgatttatcc gtgatcgggc
     3841 aaattatgcg cctacaaagg cagcgtacaa agtgtacatt attgatgagg tacatatgct
     3901 ttcaacagga gctttcaatg ccttgttgaa aacattggaa gagccaaaag aaagtgtcat
     3961 attcatctta gcaacgaccg aacctcacaa aataccagct acgatcattt cacggactca
     4021 gcgatttgat tttaaacgga tcaatacggc agatattgtc gaacatcttg aatttatctt
     4081 gaaagaatcg actatcagct atgaaccagc tgcattgaat gtgattgccc gttctgcaga
     4141 aggcgggatg cgggatgcat tgagtattct tgatcaggca atctcgttta gtgaaggaac
     4201 aattacttta gatgatgcca tgcaagtgac aggtagctta actgacgaaa tgatggacag
     4261 ttacttgagt gcttgcgtga aaaaagaagt accagaagca ttggaaacat tgaaccaaat
     4321 tctcgcagcc ggcaaagaat cacgtcgctt tctagaggat atgttacaat actgccgtga
     4381 cttgctgatg tatcagcaag cacctaacct agttgaggaa caatcgagta cgatcagaag
     4441 tacatttaca gaattggcac aaactgtttc agcggatcag ctgttttcaa tgataaagat
     4501 tttgagcgat actcaaaatg aaattcgttt taccaatagt gccgctattt atttggaagt
     4561 cgctacagtc aaacttgcac agctaccgtc tgcttctgtt ccttcagcag atgtagcatt
     4621 tgcagatgat cagatagtaa aacagctaca acaagagatt aaggaactaa gaaacgaaat
     4681 caaggaacta cgcagtaata caggaattga gaatacaaaa gtacagccgg tacagcaaac
     4741 aaatcataaa aaacagacaa gtacctatcg tgtaccgacg gaacgagtat ataaagttct
     4801 gcgggaagcg acaagacagc atctgaacca agtaaaagaa gtatgggaag acttgctgat
     4861 gagtctatct gtcactcagc gagcaatgct taaagcaagc gaaccagttg cagctggacc
     4921 tacaggacta gtgattgcgt ttgattatga gattgtttgt caaagagcag caaatgacga
     4981 agaattgcag ctttcggtac acaacaactt aagccgaatg atcaaagatt atgcgcctga
     5041 tcctgtttat attacaagag aaagctggcc agaacttcga agaaattatt tgacgcaggc
     5101 acaagaagag ggaacagaaa actcttttgc ttctgaagac tcagaaggag aaatcgagtt
     5161 gcttcctcaa gaacaagaag taccagaaga aacacttgta gtaaaagcag aggaactgtt
     5221 tggctccgaa gctgttacag tggtagatga ttaataaaaa ggagtagata attatgatgc
     5281 gtggaatggg caatatgcaa ggcatgatga aacaagtaca aaaaatgcaa aaagaaatgg
     5341 cagaagcaca agagctgcta aataaaacag agtttacagg taaagccacc aatgaattgg
     5401 tcaaagcggt ttttacaggc gatcgtcgca tgaaagatat ccaaatcaat gaagaagtcg
     5461 ttgatcctga tgatatcgag atgcttcagg atcttgtcgt aatggcagtc aatgatgcga
     5521 tcgcacaaat tgatgtggaa actgagaaaa aattagggaa atacgcaaaa ggtttgccag
     5581 gattttagtt ttaaactgaa ggagtaatga atatgcaata tccagaacca attgcgaaac
     5641 tgatcgacag ctacatgaaa cttccaggaa tcggtcagaa aacagcgaca agactcgcct
     5701 tttatacgat cgatatgaaa gatgaagtag taaatgaatt tgctaaatct ttgttaagtg
     5761 taaaacgcga tttgcatttt tgtagtatct gcggaaacat cacagaagaa gatccctgcg
     5821 aaatatgtag aaatccttca cgtgatcgaa gcgttatcct agtggtcgaa gagccgaaag
     5881 atgtaatggc aatggaaaaa atgcgagaat atcacggact ttatcatgtt ctccatggtg
     5941 tgttatcacc gatggaaggg acaggtccag aagacatcaa tatttcttct ctgttgcagc
     6001 ggctgcatga tgcagatgta aaagaagtga ttatcgcaac aaatgcaaca acagagggag
     6061 aagcaacagc tatgtatctc tcacgtctga tcaaacccgc aggtatcaaa gtgactcgtc
     6121 tagcacatgg tctgtctgta ggctctgata tcgagtatgc agatgaagtt actttattga
     6181 aagctgtcga aggaagacgt gagttataaa tttctaatta agtgtttttt cagatacaat
     6241 ataaaagcag cacaagagat gggggcaaca atgtgaacgg actatttatt acaatcgagg
     6301 ggcctgatgg tgcaggaaaa accagcatct tgaatgaact ttaccctctt ttgaaagaag
     6361 tagcaaaaac agaaatcatt caaacaagag aaccaggagg cattccgatt gctgaagaga
     6421 tccgagcagt gatccttgat ccaaaaaatg atcggatgga tgaacgtacg gaagcactgc
     6481 tttatgcagc agcaagaaga caacatctag tcgaaaaggt tctgcctgct ctggcaaaag
     6541 ggaaaatcgt tttatgcgat agatttgtcg acagttctct tgcttatcaa ggtgcaggcc
     6601 gacgcatcgg cgttaccgag attgctcggt tgaatgagtt tgctacagaa ggtactacgc
     6661 cggattttac cctttatctt gacgtagatt cagacaccgg attaagaaga atcaagaaaa
     6721 acagacaaaa tcaaatcgac cgtttagatt cggaaggttt ggaattccac cagcgtgtac
     6781 gtcatgctta cttaaagctg gcagaagaga atccggaaag aatccacaaa gttgatgcaa
     6841 gaaaaagctt tgaagaagtg ctgcaaacaa gttatcatac aattattgaa cagtatccgc
     6901 aattttttga aaactaggaa ggtgaacgtt tatgaaaatt attttagcga ttatccaaga
     6961 caaagacagc aaccgtttat caaatgaatt gattgatgca aatatccgtg ccacaaaact
     7021 atcatcgaca ggcggcttcc ttaaggctgg aaacagtacg tttatcgtgg gtattgaaga
     7081 tgaccgtgta gaagaagcat tggaaatcat caaaaaaacg tgtgaatcaa gaaagcaatt
     7141 tgtatctaca cctgttacac tggatatttc gatggatggg ggcgtccctt atccagtaga
     7201 agtagaagta ggcggtgcca cagtcttcgt cttacctgtt gaaggattcc atcaatttta
     7261 ggaggagctg agctatgaat caagaaataa acttggctca aatgcaaccg atcgtgtatc
     7321 agcagcttca acggagtttt gagcatgggc gtatcgctca tgcttatctt tttgaagggg
     7381 agaaaggaac aggaaaacat gaagtaggta tatggcttgc acagcacctg ttctgtaccc
     7441 aaatgaaaga caatctgcct tgtggaaaat gcaacaattg tcagcggatc gaaaagaaag
     7501 aacatccaga tgttttggtt attgaaccgg aaggacaaac catcaaagtc gatcagatca
     7561 gacgtttgca gacggaattc agtcgcagcg gttacgaatc aagaaaaaaa gtttttttga
     7621 tcaaagaagc tgaaaaaatg aatagcagtg cggcaaacag tttgttgaaa ttcttggaag
     7681 agccccctgg agatttttta gcggtgttag aaacagatgc agtaggaaga attctcccta
     7741 ctattcaatc tcgttgtcaa gttctgcatt ttcaagaact ttcgaaagaa gcactgatcc
     7801 atcgattgca agaagagcag attccattgg aaaaagcaaa acttttggct ttcttaacaa
     7861 acagtttagg aaaagcagtt gaaatctctc aagatgaatg gtttaatgat gctaaggact
     7921 tgatttatca atggttcgtt tacttgcaaa agcaagatac tcaagccttt atctatgtcc
     7981 aaaaaaaatt ggtaaaatca tttaaagaaa aaacacaaca gtttacagga ttatctgtct
     8041 tgatgtttta ttatcaggaa gcacagcgtc aagcagtagc aaacggcgaa tatggcaaag
     8101 tgaaacagat aaaccagata attgaacgga tattgatcgc agagcagaaa ctacgcagca
     8161 atgtcagttt tcaggctgtt gcggaacagt tcgttttgca tacgattttt tagcttgccg
     8221 caaaggatga tgatcaagga ggatggaaat ggtagaagta gtaggtgtcc gatatagaga
     8281 tgcaggtcat atctattatt ttgcaccagg agactctgaa tatgtttata gtgacaaggt
     8341 attagtcgaa tctcagcaat ccttacatat tgctactgtt gtccttccta aaaaagagat
     8401 tgatcccaat gaccttcctg aagctatccg tccgatcttg cataaagcgt cacaagctga
     8461 attagaaaaa gacaaaaaaa acaaaaaaga tgcttatgat gcattgaaaa tcgcaaaaga
     8521 aaaagtgcag ttgcgtgagt tggaaatgaa aatggtcaaa gccgaataca catttgatcg
     8581 aaataaattg attttttctt tcacagcgga cgggcgaatt gactttagag aattggtaaa
     8641 agatctggcc gctgtatttc gtactcgtat tgagcttcga caaattggtg tccgcgatga
     8701 agcaaagata ataggcggaa ttggtccatg tgggcgtcca ttatgctgtt cttctttttt
     8761 aggcgatttc gtgcctgtat caatcaaaat ggcaaaagat caaagtttat ctttgaatcc
     8821 aacaaaaatt tccggtttat gcggacgctt gatgtgctgt ctgcaatatg agagcgaaga
     8881 atacgagaca gcgaaaaaag aactccctga ctttggaaaa gtaattgcta cacctgatgg
     8941 taaagggaaa gtcgtagggt tgaatctgct tagccgtatc gttagtattc ggctgactgg
     9001 acgggaaaat cctgtcgaat atgaatggga tgaattgaaa gaatttatcc aagtaggtga
     9061 gatgaatgga taagagatca ttatacgacg gattgaatca attagaatca gagttgagaa
     9121 atacattaac agaacttgtt gaaatgaagc atgctttgca tgagatcgta gaaaaaaata
     9181 caactttgga gatggaaaat caacgtctgc gggaacacct gcgggaattg aaccaagttg
     9241 cacaagctcc agctgaaaat gtgaagcagg aactttctaa gtctcgtatg aatcttgaaa
     9301 aaatttatga agaaggattc catgtctgct acgatttata tggatcaaga agggacaatg
     9361 acgagccttg tgcgttttgt ctagaagtga tctatcgcga aagcggtcga tgaccaataa
     9421 caagaggaga ctggagcgga gaaatactgc tccagtcttt ttattaagga gattgtgaca
     9481 gatgtataat caaaaaagtt tcaagggaaa tccggtatat ggggatttat atcttgtacc
     9541 gacgccaatt ggaaatttag aagatatgag ctacagaagt gttcgtatgc tgcaagaatc
     9601 cgatcttatc gcaagtgagg atacgcgtaa tacacaaaag ttattgaatc attttgaaat
     9661 ccatacaccc caaaaaagtt tacatgaaca taattataaa gaacgtgtcc ctcaattgat
     9721 tgctttgtta aaagaaggaa aaacgattgc tcaagtcagt gacgcaggaa tgccttccat
     9781 cagcgatcct ggacatgagc tagttttagc ctgcataaaa gaagggattt ctgtgatctc
     9841 tatcccagga cctacagctg ggatgacggc tttgattgct tcagggctag tgcctcaacc
     9901 atttttattt tatggcttcc ttccacgtaa gaaaaaagaa cagaaagaaa cattgaaaaa
     9961 gctaaaagaa cagacagtta cgctgatttt ttatgagtca ccttatcgga tcactgctac
    10021 attgacgaat ttcttagaaa tttttggaaa tcgccaagtc gttttgtgtc gggaattgac
    10081 aaaggtccat gaagaatacc ttagaggaag tacggaagag ctactcgctt atatcgaaga
    10141 acatccagta aagggtgaat gttgtctgct tgtagaaggg aatttgttcg aaatgccagt
    10201 gacggaagtt gatgaagagt tgggaacgtt aaaggaacaa gtagaagcaa agatcgcttc
    10261 aggcgaaaaa ccaaatgctg ctatcaaagc agtagcgctg aaaaatgggc tgaaaaaaca
    10321 agaagtctac cgtcagtacc atgaattaga ctgagatagt ataagttgtc tataatgaaa
    10381 gactaatgag gaaatcagct actaactttt caaatctctt gaacgcgaac gaatgttctt
    10441 gtataatgaa taagaggtga ggatgatgga gctgcaattt cctttacata atgatacatc
    10501 aagaaaaatc attcatattg atatggatgc ttttttcgct tcggttgaag aacgagatca
    10561 tcctgagttt cgcggacatc cattagtaat tgccagacac cccagtgaca ctggtgggcg
    10621 tggagtagtg actacagcaa attatgaagc aagaaaattc ggaattcatt cagcaatgag
    10681 tgcacaaaaa gcgtatgagc tttgtccgca agccatcttt aagcctggcg accatcataa
    10741 atacagcgat atttccaaac aagtacggga agtattctat cgttatacag atattgtgga
    10801 accagttagt atcgatgaag cgtatcttga tgtgaccgaa aataaaatca attgcaagtc
    10861 agccatcaaa atcgcccgaa tgatccaagc agatatttgg gagagtgtcc agctaacatg
    10921 ttctgcggga gtaagttaca ataagttttt ggccaaatta gcatcagact atcaaaaacc
    10981 tagaggaatc acggtagttg cccctcaaga cgctgtgcca tttttaaaag ctttgccaat
    11041 tgaaaaattt catggggtag ggaagaaaac tgtacctaga atgcatgagc tagggattta
    11101 tacaggaaaa gacctatatg aatgcacaga aatgatgttg attcgaaatt ttggaaaaat
    11161 gggctattct ctttatcgta aagtcagagg aatccatgat tctccagtaa acgttacaag
    11221 agagcgaaaa tctgtgggaa aagagcatac ttatgggcag ccgttgcaaa cggaagaagc
    11281 agtgctgact cagttgcgcc aactagctga aaaagtagaa gaagcactga gaagagtcca
    11341 aaaacatggg aaaacagtag ttttgaaggt caggtacacg gactattcta ctgtgaccaa
    11401 aagagtgaca ttgccagaat atatctataa aaaagaagca ctcttttatc aagcaagcct
    11461 tatatgggag gaaatcttgg gagtagaaaa aggaatccga ttgttaggga ttactttgac
    11521 gaatctggac ccgatgactt atgaaaacat cgtccttcca ttatgggaaa atcaagaaat
    11581 ataaaaacag ctttgagagt tgaaaccgg
//
LOCUS       DAXWLF010000087        11484 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXWLF010000087 DAXWLF010000000
VERSION     DAXWLF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.087"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(140..2221)
                     /locus_tag="ACGE4U_001703"
     CDS             complement(140..2221)
                     /locus_tag="ACGE4U_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8393868.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(2363..2485)
                     /locus_tag="ACGE4U_001704"
     CDS             complement(2363..2485)
                     /locus_tag="ACGE4U_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393869.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            complement(2745..3104)
                     /locus_tag="ACGE4U_001705"
     CDS             complement(2745..3104)
                     /locus_tag="ACGE4U_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFF8393870.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3134..3475)
                     /locus_tag="ACGE4U_001706"
     CDS             complement(3134..3475)
                     /locus_tag="ACGE4U_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393871.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3472..4146)
                     /locus_tag="ACGE4U_001707"
     CDS             complement(3472..4146)
                     /locus_tag="ACGE4U_001707"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8393872.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5080..6378)
                     /gene="asnS"
                     /locus_tag="ACGE4U_001708"
     CDS             complement(5080..6378)
                     /gene="asnS"
                     /locus_tag="ACGE4U_001708"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFF8393873.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6418..7608)
                     /locus_tag="ACGE4U_001709"
     CDS             complement(6418..7608)
                     /locus_tag="ACGE4U_001709"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8393874.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7629..8156)
                     /locus_tag="ACGE4U_001710"
     CDS             complement(7629..8156)
                     /locus_tag="ACGE4U_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFF8393875.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8203..10992)
                     /locus_tag="ACGE4U_001711"
     CDS             complement(8203..10992)
                     /locus_tag="ACGE4U_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFF8393876.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11139..11339)
                     /locus_tag="ACGE4U_001712"
     CDS             complement(11139..11339)
                     /locus_tag="ACGE4U_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8393877.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT         3156 a         2498 c         2007 g         3823 t
ORIGIN      
        1 ttattgaatt tcaagctatt ttgaattttc tgcaaaatta aaaacattag ttattttata
       61 atcctatcta ttttctttac atttttcatt aagaaaagga tataaaaaaa agaggacgtt
      121 cattcgtcct cttcattgat caacgtttat tttttacatg caagcttcgc atggcattca
      181 aaacagctaa tactgtcacc ccaacatctg caaatactgc atctcccatt gatgcaaaac
      241 caaaagcacc taaggaaagt accaaaattt ttatcccaat agcaaagaca atgttttgtt
      301 tgacgatttt caatgttttc tttgctaaat ggattgcttc tgcaatcttc gatggttgat
      361 cattcatgat gactacatct gctgcttcaa ttgcagcatc gcttcccaat ccacccattg
      421 cgattcctac atctgctctt gctagcaccg gtgcatcgtt gattccatct ccgacaaatg
      481 cggtcttttt attgttactt tgtaagattt cctctaaccg ctgcacttta tcttctggaa
      541 gcaattcgct atatatcttg tccacgccta cttgcttccc gatatgatca gcaatttttt
      601 tcgagtcgcc cgtcaacatg actgtgtttt tcacaccagc ctgttttaat tgaaccaatg
      661 cgtctacggc atcttctttc aaggtatctg caataacaag ataaccactg taactttgat
      721 cgattgctag ataaagcaat gtaccgattt cttgacttga aggaaaagag atttcaaatc
      781 tttccatcaa tttgtgatta cctactaata cttgtttgcc atcaacttca gctaagatcc
      841 cttcacctgc aaattcctgg atattagctg ttggtaaaag cggacctgcg tagccttcta
      901 aaacagattg agcaatcgga tgtgtagaaa actgttctgc actcgcaata tattgcatga
      961 atatctttgg atcgattttt gtatcgatcg tctggatcga aaagtcgcct ttcgttaatg
     1021 tgccggtttt atcgaatacc actgtctcgg tttgcgcaag caactccaag taatttgaac
     1081 cttttactaa tacaccgatt ttacttgctc ctccgatacc gccaaagaaa ctaagtggca
     1141 cggaaatcac taatgcacat ggacatgaga tcactaaaaa tgtcaatgct cgatagatcc
     1201 attcagaaaa cgccgctcca gaaacaacaa gtggaggtaa aacagctaat aaaacggcca
     1261 atcctacaac gactggtgtg taatagcgcg cgaatttagt aatgaaattt tcagctggtg
     1321 cttttttact gctggcattt tctactaatt ctaataattt gctgatagta gaattttcat
     1381 aagatttttc tacttgaact aaaaatggtt tatttttatt gataaatccg cttaatactg
     1441 tttcaccaac atatacagat ttagggactg attctcctgt aagtgctgac gtatcgacta
     1501 atgattctcc ttcgatgatc gttccatcta acggtacacg ttcaccaggc ttcacaagta
     1561 caaactgacc gatcagcacg tcttcagggt tcacggtcat caatccttcc gccgtctgca
     1621 cgtgagctgt ttctggacgg atcgccatca attcgcgaat cgattttctt gaatgactga
     1681 ctgccaatcc ttggaaatac tcgccgattt gataaaatag catcactgct acggcttctg
     1741 gatattcacc gatcaacata gcgccaatcg tagcaacaga cataagaaaa ttctcatcaa
     1801 agatttttcc tttgacgata ttcatcactg ccttcttcac tacatcatat cctatcagca
     1861 gataaatcaa taaataccct atcaagcgaa ttagttcatt aaaatcaaat aatagcaata
     1921 aagcgatacc tgcaagggaa aataaaatcc ggaacagcgt atttctctct ttttcatttc
     1981 catgttcaga tactttttcc ttatctattt ctgttacttt gacatctggt tctaattcct
     2041 tcactacttt ttttgtttct tgttcaatcg tagccaaatg atcttcctcc gcgtcaatcg
     2101 ttagttttcc agaagtgaag tttaccgttg cttccttgac tccattgatt ttctgaactc
     2161 ctttttcgat tttcattgca cagttcgcac aatctaaacc ttctaatctg taacttttca
     2221 ttttaatgac ctcctcgcct caacatatga atatctcttc ataactaatt ataaatgaat
     2281 ggttattcat atgtcaaggt atccgaaaga aaaaatgaaa aacatctgca tcggaaaatt
     2341 tcttccatac agatgttttg gcttattctt tctttttctc ttcgatcatt gccactgctc
     2401 ttatccaacc cgcacttgcc ttcttgatct tgacaggata gcaactaagt ttgaagcctg
     2461 ttgctggcaa tttgtctaaa ttcattaatt tttcgatagg acaatatctt ttttcaatcc
     2521 ctgcaaaatg tccttcccag atatacgaag ctccactacg ctttcaaaaa tatagaaaga
     2581 gttctttaat gaaaattatt tcaactttcc ataaacagta accccatata aataaaagac
     2641 gtaaaagtca catggaagaa cttatacagg aaaacgcata gagtcaggtg aaatacatgt
     2701 atcccacctg actctatgct ttatctaacc tttattattc tcttttatac aatttctgca
     2761 ccgaatggca tcaacgccaa tcgagcaagt ttgaaagatt gcttggcaaa aggtatccct
     2821 atgatcgtga tacatagaag gatgccgctg acgacatgtg cgagagcaat tggtaggcct
     2881 gaaacgatca gccagatgat attaacgatc aatgacatgc cagactggtg ataaattact
     2941 tctttgccaa aaggcgcaaa agaaaggaag gcgatcttaa aacattgcat accgattggg
     3001 atacctatga tcgtgataca ccaaagtatc cccgctgcta accagctaag accaccgatt
     3061 agtccgccga atataaacca gatgacattt cctaaacaac tcattgtatt tctccttcta
     3121 ttactgtttt tgattaaaac tgggcaaaaa ttgattttat tcttcttttt ttagcttcca
     3181 gcatcaaata ttctgcaaat tttttttgga tttcttccgt attttctttt gttactaatg
     3241 tgtgttggat tagttttcct gatggtcgct gacccacccg ttcttctgac tgttgcacgt
     3301 ccgcttgcgc aaaaccgata atccgttttt cttttgttac agcaacgagc aatagtttat
     3361 tcttatcatt taggtcagct gccgtttttg cttttacgaa tgattctgga tgatcagtaa
     3421 tcagctgatt gatttgaggt agatcttctt ttactgccca ccgtatttcc atcatttctc
     3481 acttttcttt cgctcatttt tatctccact aaaaaaatcg actgcggcaa ttgcatctat
     3541 ccagctttgt gcaatgatct catgtccgcc aatcgttggg tggaccccat cttctcttgt
     3601 atatttcgaa aaaccataag caatccctaa agcattgaat atgccatcta aaggaaccag
     3661 caatgcgcca tattccttag caagtttacg aacaatttgg attttagggt ctaagtcttt
     3721 acgccagcca tgacggtcgt cggttatagg aagtacaaac ggctccatca agatgatgtt
     3781 gcagattccc gctttttgaa gttcatttag taattttcga taaacggatt caaattgctg
     3841 agcagatttc tcagttccga atatagcctg atcgctagta ttatgccagg tatcattgat
     3901 tccgatcaaa atcgatacat agtcaggttg taaagctaaa caatcttctc gccagcgtac
     3961 ttgcagatct gcaattttgt ctcctcctat cccacggttc atgcattcaa attccatcgt
     4021 tggatattgg gagattagtt ttccggcaat caaaagtgga taacctttac ctagatcagt
     4081 tgcttctgct cggtctcttc ctgcgtctgt aatgctgtca ccgataaata atatacgttt
     4141 tttcatttgt caaaaactcc tttttgaata aaacgatgat tttcttcctt atattatccc
     4201 ataagcccta tcaagacgaa agagaaaata tatgtataaa aaatgacacc agcctagtcc
     4261 cttcaaacaa ttgaatgaac gctcatcaag tgtctagttt tttatgctat ttttttctta
     4321 acgtgctgga ttccccttga ttccttcatt gtttggtcca gagagatcga tcacacctaa
     4381 gcttccgtca atcagaccac ttgatgacgc catcagaaca tatatttata ggctcatcta
     4441 agacttccag taaatgttcc tttactaata tctcttctag attgcttggg atagattggc
     4501 cataacttta tctggtccga tgatttcaag ggtgaattga acttacatat ggatcattgc
     4561 atgctgaaaa tacggaccta ctccatcttc gaatgttact attagtttgt tttcaggtgc
     4621 tagatgctgc tttatttcaa cagcagaagc atcatcaaat cagatttcca tttgaataac
     4681 cttctttgta tatgtttgat tgattgcgat ttcgttatag aaccttgtga atcaaatagc
     4741 aatttctttt gctttcttct cttatctgct ggaaacttcc tttatcccgt atttaaaaaa
     4801 aaagaactgc gagagttcgc agttcttttt tttaaataaa cgttgctctc cggccaactc
     4861 cttggtcaca agtcacaata gtcatcaaac atgaaaaaat gtttttttga aattactcct
     4921 tgtgactcgg agctgaacgg cagacgcaca acctcttgat ataaacggtg ttcaatcagc
     4981 tgaccctcga tattagctca aaaaacagta aaatatgaga agctattttt ccgttttgtt
     5041 tctctaatac tcaggtcaag gcactgattt cacaacctct tatggataaa ttcggttcaa
     5101 caaacgtggg aatggaatag cttcacgcac atggtcgata ccagcaatcc aagtgactgt
     5161 tcgttcaagg cctagaccaa aaccagagtg cggaacagaa ccgtatttac gtagatcaag
     5221 ataccatgaa tattcttttt catccaaacc gtgattacgg atttgttcta gcaagaaatc
     5281 atagtctgtt gcccgttcgc ttccaccaat aatttcaccg taaccttctg gtgcgatcat
     5341 atctgcacag atcaccacgt cttctcttgt tggatgtggt ttcatgtaga atggcttgat
     5401 ggcttttgga tagtttagga taaagacagg ctgttcgaat gaattcgcaa tgaacgtttc
     5461 atgaggtgaa ccaaagtctt cgccccattc gatgtcatca aatccatttt tcttcagtaa
     5521 ttcgatcgca tcatcataag taatacgagg gaatggcaat tttgtataag tttttaagac
     5581 ttctttgtct cgtcctagta catctaatgc gtattcgcaa tgatctaaaa cattttgtac
     5641 taagaatgct acatattgtt cctgtacttc taaactttct tcttgatgag tgaaagccat
     5701 ttctggttcg atcatccaga attcgatcaa atgacgtcgt gtttttgatt tttcagcacg
     5761 gaaagtcgga ccgaatgaaa agacttttcc gaaagccata gcagcagctt ccatgtataa
     5821 ttggccactt tgagataaat aagctttttg atcaaagtaa tttgtttcga atagatccgt
     5881 agtgccttct ggtgcagaac cagtcaagat cggcggatcg attttgacaa aatgatgttg
     5941 attaaagaat tcatacgttg cacgaatgat ttcatttctg atctgcataa tcgcatgttg
     6001 tcttgaagaa cgcagccata aatgacgatg atccatcaag aagtctgttc cgtgttcttt
     6061 tggtgtgatc ggataatcgt ggctctcgct taccacttcc acatttgtaa ttccgatttc
     6121 ataaccaaat tttgaacgac tgtcttcacg gatttctcca gttactaaca cggatgtttc
     6181 ttggtgtaga tttttagctg tttggaaaac ttcttccggc acttcgcttt ttacaactac
     6241 accttggaaa aaggctgttc cgtctcttaa ttgcaaaaat gcgatttttc cgcttgatcg
     6301 cttatttgcg acccaagcgc cgattgttac tgtttctccc acatggtttt tcgaatcgat
     6361 gatttgaatt tgttccacgt atgactctcc ttttatattc cttgaatttc ttcaggatta
     6421 attttgactt ttactctcaa taaattgctt gattctgcgt accgcttctt ctaacgtagc
     6481 gcgatcagtg gcataactca tgcggacatt ttctggtgcc ccaaagcctt cgccagtcac
     6541 aagcgctacc cctgtttctt ctaaaagatc ttctacccat tgtgtgacat tttcatattt
     6601 acacatgcgt agcgtttctt ttacattagg aaaaagataa aaagctcctt gtggcttttc
     6661 aagagaaaca cccggcagtt ctgccacaag cggatataac gtattcaggc gttcttcaaa
     6721 ggcgttccgc atttcttcca ctgtatcttg ctcgcctttc aatgcttcaa cagctgcata
     6781 ttgactgact gctgtcggat tactggttga ctgggaagca acagcaatca ttccattgat
     6841 gatctcagaa ttccccaccg cataaccaat gcgccagcct gtcatcgcat atgttttaga
     6901 cacgccattg atgatgatgg tctgattttt gattgcttct gaaatcgttg caattggagt
     6961 aaactcattg ccattataaa ctaaacggcc ataaatatca tcagcaacta tcaaaatatc
     7021 attttttact gcccattcac cgattgcctg cagttcattc ttgctataaa tcattccagt
     7081 cggattcgat ggagaattga tgattacggc ttttgttttt tctgaacgtg cttgttctag
     7141 ctgctcaaca gtaactttcc agctattgga ttcttcccct ttcacaaaga caggaagtcc
     7201 ttctgccagt tttacttgct caccgtaact gacccagtaa ggcacaggaa tgatgacttc
     7261 atcttgcggg tcaaggatcg tttggaaaag ggtatacaaa gcaaatttcg ctccgtctgt
     7321 tacgactgtt tgtgaaggtt catagacaag tccatagttt ttcttcagat aatcaacgat
     7381 tgcctgtcga agctctggga ttcctgctgt tggtgtataa tagctagctt tcccattgcg
     7441 gattgcttcg attgctgctt cttggatgtt ttctggtgtc gcaaaatccg gttctcccac
     7501 agtcaaactc agcacatctc tgccttttgc ttttaatgcc ttggcctttg ccgcagctgc
     7561 caaagtgaca gacggttcta atcgttttgt tctttttgat aactccatcc cattcattcc
     7621 tttacctttt aaattccctt gattgtcttg acttcatctc cagattcaaa gccaatcagg
     7681 taataattca gtttcccatt tgtttctttt cctactactt cccacacagg ctgatcatca
     7741 tacatcccta aagcagcttt ttcgatttgg atttcaggat gggccgcagc aattgtctgt
     7801 tttgcttcgt tttcagtcag tccctttgtt tgatccaata cttttacttt ttcaccagat
     7861 ttaggaatga taacggcgat ttcttttcct ttatcattcg tacccgtcaa tgaaaagtac
     7921 gttttctttc gagtaaacca gtaaaaatga tcggcttcct cgagatttgc atatttcttt
     7981 gcgatttcta ctgcttcttt tttcgcctgg accgccggac gactggaacg caagtaaaaa
     8041 atcgagctga tcacgatgac cgctaataaa aatacgatca agccaagcaa cacgcgatta
     8101 atcgtttttt cctttgtctg ttttttcttc aagggtcgtc gctcctttat cttcattcct
     8161 tttcattata gcagatttta atgatgaaaa aagacggcac ctttaacttc cttcttcatt
     8221 tttctttaag aattcatgga tatcctgata gatttcatct atagctagct cttttatcgg
     8281 tacttttttc ggtaatgcat ggatgattcg tgcaccatat tttgcagtga ctaatctccg
     8341 atccaaaata agtaatacgc cacgatctgt ctccgtcctt atcaaacgac ctaatgcttg
     8401 gcgtaacttc aaagctgctt taggcacgga ttcctgataa aaaggattga tcccctctga
     8461 ttccagataa gcattcctag ctttgaccat cggacgtaac gggttgtcga agggtaaacg
     8521 tgtgactaca acaatctgca aagcatctcc aggtaaatct acgccttccc aaaagctgtc
     8581 agcaccaaat aagacgcttt tttctgacaa aatgaagcgt ttcaaaagtt tttcccgact
     8641 cccgccaaat ccttgagcaa gaatttcacg tcctttttct agcatagaca aatgcatttt
     8701 ctgatatacc ttttgcaaaa catcatgaga agtgaataaa acaaggatcg gccgatcttc
     8761 ttgttcaatc aatcctgtga gtgctgcttc taaataatcg atatatgctt gattgctgac
     8821 ttgatggact gcaggtgctt ctttcggcac atagattctt gcctgtttct cataatcata
     8881 aggagaagca atcactttaa gaggtgctgc ttcaatgcca agtcgtttag agagatactg
     8941 tcgattaggt ccgatcttca gtgttcctcc aatataaaga atatggtcat atcgaggata
     9001 ccacacagtt gacggcaaca tagctgcttg aaaatcaagg agatgaaaaa tcatctgctg
     9061 tgctgtattc ccgggaacga accaatggat caaatgatct cgccagtctt ctagccattg
     9121 gtccatcagc aagtattgct tgtctaattg atcaaataag ctatataaac ttgctaaatc
     9181 gatccgcatc tggttggtcc aactttcttg gataatctca aaagtcaagc gcatctgtga
     9241 ttgtagttgt tggatctctt gataaaaaag ttgcagatga tccattgctg ttccagattc
     9301 cagtggcagc tgatccaaca tttcttttgt catcatgact tcttctggat attgtccgtt
     9361 aggttgcttg ctgcttacta agttcattaa tccctctgcc acgcaaatct gcgcttcgca
     9421 tagtgcagag agttcttctc gataaatcga caaccagcgc aaggcttcat gttccttcgg
     9481 caaataactt tgtatgcggt caaataactg attcggctct tccagttgct gaatttgttt
     9541 atggaagtag ttaatactta gtcgtttgtc atttactttt tcagcaattt ccggcaaatg
     9601 atgcacttca tcaatcaaca aataaggact agctggaagc aatggttctt ttcgctgtgt
     9661 ttcttgtact aaaaatgcat ggttgactat caaaaaattg ctttgtttga ttttcttttg
     9721 caagaaacgc acaaagtctt gtttgtaaaa tgactgattc cttgctaaaa acccagtgcc
     9781 tctgtgcgcg acatctgcaa agaatgcatg gttcagattg gttaaatgta attcatccaa
     9841 atcaccggtc ttcgtttgtg ttagccaaac taagacagcc atttggtaca aagcatattg
     9901 tttttgtccc ggcggttcca caagtgtacc tttaaaacgc tgtaaatcaa tatagtggcg
     9961 atagcttttg ataatcacag gttgtatcgg ctgatctagc aattgattca gtaatggaat
    10021 gtctttttcc atgatctgct gctgcaaaac aagcgaaacg gtgctgatga cagctggttt
    10081 ttcaggtgta gccaaataac tcaaaggcaa cagataacca atcgttttgc ccattcctgt
    10141 agctgcttcg atcatcaaat cttttgattc atcttttgtg aaatggtcgt agacagcatt
    10201 catcagcctg ttctgttctt ttcgatacat cagtgtttta ccaaacattt tttcttttgc
    10261 ttgttttttt cttggatatg tccgctcgcc ataatggacg gatgtgaata gctccacttc
    10321 ttttttctgc aaagctagcc catcgatgat ttctaatgag agatcaaggg gctccgggtt
    10381 ttctttcatt tcttcaagca cgtggtaaat gaaacgactc gtatccattc ctgtatggct
    10441 acttaatcgt gcaatctgct ctaatgtgat gatcggcaat ttcctcattc gttcttcgat
    10501 cagtaaaaga agctgacctg tcacttcggc atcactatcc gcctgatgag gattctcatg
    10561 aataaagcca aggctttctg acaaatcagc taagcgaaaa cttggttccg tcggcaagaa
    10621 aatttgtgca agttctacgg tatcgatgcc cggtatcttc aaactaggaa gtccgcatcg
    10681 catgagttca tggttcaaaa aattataatc aaaataaata ttatgcgcga caaatatggt
    10741 gtctgccaaa agattgtaaa tcgtttgggc gacatcttca aaataaggcg ctttttggac
    10801 gcgttgattc gtgatccctg ttaaatgctg gatttgtttt gaaatcttcc gccctggatg
    10861 aatatcgatc gaaaaacgtg aagtgatccg accagcttca atcatgacgc agccaaattg
    10921 gatgatccga tcttcttttg gattcgtccc agtcgtctcg atatcaacga ctgcataagt
    10981 tcttggtttc aaaatatctt cctcttctcc tgcctctaat gttcttttga aattatacta
    11041 caaagtgcag aaaaatactc tcatcgcttt ttaaggaaaa aaagacaaaa aagcccagac
    11101 acataaatgt ctggactttt atttttaata taggtaaatt aagctttagt aacgtttgtt
    11161 gcttgaggtc cgcgttgacc ttcttcaacg tcgaaagtta ctgcttggcc ttcttctaaa
    11221 gttttgaatc cgtcgccttg gatagctgag aaatgaacga atacatcgtt tccgttttct
    11281 gcagtgatga atccaaatcc tttttctgag ttaaaccatt ttactgtacc tgtttccata
    11341 ataaaaatcc tccttgtgta tcaacacata ttttgcaatt acttgaatgg tgtatatctg
    11401 gaagatgttt atttgaaaca agctaccaca gattaccgaa caaaaactac attcttacta
    11461 tatcataccg tttacaaaat tgcc
//
LOCUS       DAXWLF010000088        11467 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXWLF010000088 DAXWLF010000000
VERSION     DAXWLF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.088"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            271..477
                     /locus_tag="ACGE4U_001713"
     CDS             271..477
                     /locus_tag="ACGE4U_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFF8393878.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(679..1680)
                     /locus_tag="ACGE4U_001714"
     CDS             complement(679..1680)
                     /locus_tag="ACGE4U_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8393879.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1685..3598)
                     /locus_tag="ACGE4U_001715"
     CDS             complement(1685..3598)
                     /locus_tag="ACGE4U_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8393880.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3766..4272)
                     /locus_tag="ACGE4U_001716"
     CDS             complement(3766..4272)
                     /locus_tag="ACGE4U_001716"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFF8393881.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4432..4872
                     /locus_tag="ACGE4U_001717"
     CDS             4432..4872
                     /locus_tag="ACGE4U_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8393882.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4898..5926)
                     /locus_tag="ACGE4U_001718"
     CDS             complement(4898..5926)
                     /locus_tag="ACGE4U_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393883.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(5981..6055)
                     /locus_tag="ACGE4U_001719"
                     /pseudo
     CDS             complement(5981..6055)
                     /locus_tag="ACGE4U_001719"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6058..6414)
                     /locus_tag="ACGE4U_001720"
     CDS             complement(6058..6414)
                     /locus_tag="ACGE4U_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393884.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6712..7686)
                     /gene="sgrA"
                     /locus_tag="ACGE4U_001721"
     CDS             complement(6712..7686)
                     /gene="sgrA"
                     /locus_tag="ACGE4U_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFF8393885.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7882..8721)
                     /locus_tag="ACGE4U_001722"
     CDS             complement(7882..8721)
                     /locus_tag="ACGE4U_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFF8393886.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8906..9793
                     /locus_tag="ACGE4U_001723"
     CDS             8906..9793
                     /locus_tag="ACGE4U_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFF8393887.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10387..11081)
                     /locus_tag="ACGE4U_001724"
                     /pseudo
     CDS             complement(10387..11081)
                     /locus_tag="ACGE4U_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(11065..11463)
                     /locus_tag="ACGE4U_001725"
     CDS             complement(11065..11463)
                     /locus_tag="ACGE4U_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFF8393888.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3418 a         2292 c         1718 g         4039 t
ORIGIN      
        1 aaaagtttag gatacatcga atttttgggg aaattctttt gggggtatcc taaatctttt
       61 ctattatatt cccatatttt ctattattgg tctagacgta tgatggtaaa ctttgtacct
      121 atctgattgc tgaatcatta attgcttatc attagccatc tttgtttcaa gttcgctaga
      181 tgatcatttt attcacatcc taacaaactt gtcacattta tagattctta atctatttat
      241 catacaatta taaaaaagga ggtgctgctt atgaaagcta tccatatttc tttgcttttt
      301 ttcgctagtg cattgatttt atttgctatt catctttgtt cccagcctta tatcaataac
      361 aacggaatat tagtggaacc agcattcttt tgcttaccac tcggcttttt gtcccttctt
      421 gcctccgcag gcttcggatt tgttgctttt tttaaacgat caaaacaaca aatctgactt
      481 ataaaattta cgtaaaagag atcgtgacaa taatcgtgtg atattcccaa atttcgaata
      541 aaaggtgccc cttgccatct ctaactcctc agtcacaagt tacaatattc ataaaccatg
      601 aaaagcttgt ttattgaaat tgctccttgt gacttggagc taagtgacag attacaacct
      661 cttttgcatc ataagacttt actccttgaa aaacattact aacttttgat atgtttcttg
      721 cgcttgttta ttgctgaaat caaactgata ttcatggttc aaaacagcat ttttctccgc
      781 ataatctact tttgtgactt tcacccccgc tttctcaaat acagaagtta cttcttccat
      841 ttgtttcgta aacgtatttg tatttccatc cgttaagaat gtgttttgcg tccaacgatc
      901 cgcatgtctg accaaggcta aacttttgac cttctctgat tgttcaaagt ccttattatc
      961 aaagtaagcc catccacatt tttcataaag aaagctagag attttatcac ttacttgagt
     1021 aagtgcagaa ggatccaaca gaccagacaa agaaataaac ttcgtaatct gcttgttctc
     1081 caaagtcgga tcaagatgat cagctttttt aatttcttca ttttgaagtg ctaacaaata
     1141 ttctccacta atttgtgcac ctgccgaatt tccaccaatc gaaatattcg accaatctgc
     1201 ttgatctttt atctcttttt tagctgcttg aatggcttcg ttcaattgga taactggtgt
     1261 aggatgtttt gcttccggtg atcgttcata atctatattt acgatgattt gctgcgtgtc
     1321 tgaacataga tattccaaat aatcacgaca atcattttta tcccctccta catatgcgcc
     1381 tccatgcatc cagaaaagaa tcggtaattt tttcttttcc actttcggtg tgtagatatc
     1441 catattatta tttttgtact ttgaaggata ggatatttct ttttttatat cgatttgttc
     1501 gattttttgc tggaaatcat ctggtcgggc atatacttgt tctttttcac ttgtgtcaaa
     1561 ttgttttcta acaagcaagc taatcggctt tggtgtgatt tgattaatca aaaatacagt
     1621 gactaaaata actacgatga taactggtac tgcaatcact attcgcttga accatttcat
     1681 tcatttaccc tctctttctt cgccaaatcc atgtgataat cgtgctaaca gataaaaatc
     1741 cacctgaata aatgagaagc gaatgtttct tttctccagt tttcggcaac attctttttt
     1801 ttgcatctgt ctttttttgt actttcactg tcatctgaat cggttgcgta tcatcacttg
     1861 attgatcctc tcgaggttca ttctttccct tattgtctgc attctctgtc ggatcttgct
     1921 tttttggtgg agctactggg atttttttgt aatagaaaat aatagttggt atcgtctcat
     1981 taaaaatccc ttcagtcggt ccttcaacat ttgctaattc gtatccttcg aatgtctttt
     2041 gctctgtcat ataattttct ccataagctc cgtaaagctg gatttcgtcc tggtctttga
     2101 gtagactttt attgttttca tccacatact ttacgtttat ctgatcatgg aacactactt
     2161 tcaaaaagtc cgttacatgt ttcgtgtagg cagcatggtc aacttcctgt gcctttccat
     2221 gccctgctcc ttctactaat aaaagatctt tataccctcc tgataactca tataattttt
     2281 gcgcgttaga cacaggtact acatcatctg ctgttccatg gataatcaat ttaggcaatg
     2341 aatctgacat tccaatcgta ggtagagcag cttttgtatc caaatgcatc tcctgatcaa
     2401 aatagtattg tttcaaaagg cccataaata aatcttcatg ctcttgatcc ccaatgatgt
     2461 ctaactgatt ttttatggca tccggtagtg cgggggctac tgtctggctg tacgtaaaac
     2521 gtagttgttc atcaattgat gcaaatccgc aattttcaat aattcctttg acattgacag
     2581 gaatatcttt cgctaataca gacatcatcg tagctgctcc catcgaacca ccataaagta
     2641 aaatcttttg agatggccgc tctcgaactt cttgatttat ccagtaaagg acgtcatctg
     2701 actcatattg tccataggtc acataatcgc ctccgctatt accagtcgct ctggaatcaa
     2761 caaataataa attatacccg gccttataaa aatcattgta ttcttctgta acgatcccgt
     2821 tattccaatt ccctcgaaat ccgccatgga tcatgactgt tttatctgaa ccttgatcga
     2881 cataatatgc atatacatcc atcattttgc catcttgttt gtttagtaca gtttgatgaa
     2941 gttgttttgt atttggatag aagcctgtgt tttctgtagg cgttgtccca tcatctgtac
     3001 ctccaagcat cgacatcaac acactgtttt ttgcaaaagc gatatcgtac aatagataag
     3061 tagcaatcag atgtttgata atctctttat ctacaccata atcattaaca gcaagctcag
     3121 tgactaataa ctctattaat tttgacttga tgtcttctgt tggtccatta agtatttctg
     3181 tcatctgttt accgaagtaa ttcaaatatt ctttaccatt gaaattttca cctttaccga
     3241 tatcatcatt ttctgtccca ttctctttgt cacttacttt agcagaagag gattcgtcgt
     3301 ttgacatttt tgtagctgtt gcttcttctg tttgttggtt atctgtggta gcggtcgttt
     3361 cttgatctgt gctagtaaca gatgtctcaa ccgttgtttc agtatttgtt tctgtttgtg
     3421 aagtactcga tttcgtttca tttccagcat cagtagtttc tgtggtttct acctcccgag
     3481 attccgattc ggaagaaatg ctctctccat atactgtcca ttgcgggacg cttgtactta
     3541 cagcaaataa gatgaccaac gctcccttta atacttgttt tgttctcaag ctattcactc
     3601 ctttttatgt ttctaggaga attatatgta aacgcaaaca ttttttatac aaaaaataaa
     3661 tggtttatcc cataaaaata gaacaggatt attttctcat tttacgtaat aaagcagaat
     3721 ggataaacca gtatccattc tgcttttttt aaacgaagaa acaacttatt gctccaaaaa
     3781 tgtcagaaat cgtttatccc aaattttctc gtaaaaagcg attgtttcct ttgctttcat
     3841 aaaaggatta tcataagttg tcgttgctcc tctacacatc ataagctggt atcctaatcc
     3901 atgagcaaac ttcacagtcg tatctataca atactctgct tgtgccccac ataattcgat
     3961 cgaatgacag tccaattcag ttaataaaac actcaaatta gtcttataaa atgaattcgc
     4021 atgggttttt ctcacaaaat aactgtgctc aggaatagat aattccgaca atagctccca
     4081 tggttttgtt tgtggcacta attgagcatc ttcgtgttga acgaaaataa ctggtttccc
     4141 aatcgtttcg tatgttttga ttcttttatt cactaatgct gtcactgttt ctaattcgtg
     4201 tatcggcgaa gaatcaaaac ataccccttt ttgtaaatcg atcactacta ttgcgtcggc
     4261 tgtgtttgac atacttgtgc cttctttctt ttttcttgat ttaatagtat ttcttatcta
     4321 gctagttcca ttatacgtga caacattttc ttattttaca cctctaaatg gcacttttcg
     4381 actatttctt catgttatta tttcataaat acttagctat tggaggaatg tatgatgatt
     4441 aaattgtata caggttctgg ctgctcgtct tcaagacgcg caaaacgttg gcttatcgtc
     4501 catgatatac cgtttatcga acagaactta attcgtcaac cactaaccaa aaatgaattt
     4561 atccacttac taaaattgac aaacaacggc gtgaccgaaa tgctctctac tcattctatt
     4621 agttataaaa atcttgttca cagaatagat gaactcagtt tgagtgaact atatattgag
     4681 attcgtacat ccccttcttt attgaaaaaa ccgattatct acgaccaatc tgcaaaactt
     4741 ttgactggat ttagcgaaga taaaatcagg atgttccttt ctaaaactaa aagaaagcaa
     4801 gagctcaacg aactcttgct ttctggtcat cttgtcgatt ttaaagaaaa taatcttttt
     4861 gcaatgtctt aattttggca taaacaatct gcttttctta attaacgaaa tccaattttt
     4921 ccaatatgga tctatttttc tcattcctga tttttatgat ttcagctttc agacgcgccc
     4981 aatcattttt tgtaggttgt ccattccagt aaaatatctg tgtattcgtt tgatcatatg
     5041 cctttttgtc gataggataa tcgctaatga caatatcagg taactgatca gcggatgcac
     5101 tatattttat tgggcgttca gtcatagata atatcctttt ctttgcccat aattcctgtg
     5161 ttttaccata ctttgattga atatagaata tgacaggttg ttcgtgtttt ataaaaatat
     5221 ccctgactaa catacagtat tgaaaaatca accgtctgtt cttttcataa taagtattaa
     5281 gctctggaat tctgcgagct accccactaa aaaactgttg catgattgga tacatcactg
     5341 gaaatatatg ctgaaaatct ctgtcttctg ctgattttcc aaaagcatcc accttctttt
     5401 ttgtcccaaa aacccttgaa tacaaatgga tcattgccaa attgacatat agataatttt
     5461 tttctgaagt tgtaaaacaa atcttgaagt ttttttcaaa attttctaca atagacgatt
     5521 caatccagcc ccaactttct tgtactttat ctggagtatc caacaaatct tcttccaaat
     5581 aggtgttcat tacaccaaac ataaaataaa ggtaagcttg ctctgttgga gtgatgtgat
     5641 tcatcgacca gaagtttgct tcaaaaaaag gctctagtat gtaatgcgta aactcttctt
     5701 ttgttatcaa tggatgcaca aatttgttca tctcttctgg gacatacgta atcgagtggc
     5761 cttgtttcat tcgttgcaac gaaattctta gtaggctgat ccatttcttt ctatctatac
     5821 tacgcgtata tggcgcatgt ttcgcaaatt tttctaatag ttgacgttta ttttttatac
     5881 ttgataatat atcagaatga ttttcgtcga atgactgcca atacatcaag tgaaaaaagt
     5941 atcggatacg gtattcttct ccttctatcg gattaccttt tttcaagctg caagataaat
     6001 gatagttttt caaaatagta ttaagttctc ttaattttct ttgaatcgta cgcaaactta
     6061 tataattttt tgactaagat tttctaagga ttgataagta cctttgtaaa gtgaatccag
     6121 taaaatataa ccaatcgttc cctctaaaag tatagaataa actttactca gtaaaaactg
     6181 agaagaaaac tcgactttta tcatcttgtt ttctgaaaga aaagataatt tatattctgg
     6241 atacctttcg caaaacgatt ctgcttgttg aatcaccgaa ttcatcttag ccagggtgta
     6301 gtttagatct aaagcgatat ccttcaccac atataatttc tcgtctcttt cataacaata
     6361 gttcaaaaat tgatatactt gcctttcatt gatatcaata aattcttcaa acaaagtaca
     6421 tactcctttt tatctattat ttagacttat tccgacagct acatgaataa cgtacaaata
     6481 gattcgttac tgatttttca gagatactac catgcgcagt gtgcattgtc aattattctt
     6541 gctcttattt tccatttgtg ttcctgttat ttcctgtttc tcaaatatgt tagtaaacaa
     6601 aaatagatct ggcaaagcta caacttcgcc aggcaataca gcactctcga atgaaatatt
     6661 cactcaaaaa tctatcgttt tattcaattt tagatctgtt gcttttattc tttatttcac
     6721 tcgatggaag agagaacgaa aatagcttgc tcctacaatc aaaatgagta acacacccat
     6781 ccatgtaatt agcttgttca aggttctact accagtttct ggtaaatcgc tttttctatt
     6841 atctgtttct ttcgcttttt cttgatgatc aattccacga gaaacaaaat caggctgttt
     6901 tagcacactt tttaggttgc tccctgcatt tctcttttgt tccttagttg gtatgatagg
     6961 taccaaatca agttcattat ttacttttcc tgtgctgctt tcgctgctcg tgcttcctgt
     7021 gctactctca ctactcatgc ttcctgtgct actttcacta ctcgtactct ccgtactgct
     7081 ttcgctgctc gtgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
     7141 gctcgtgttt tctgtgctaa tttcactgct cgtactctct gtactgcttt caccgctcgt
     7201 gttttctgta ctgctttcac tacttgtgct atccgtactg cttggcggtc caacaactgt
     7261 ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa aaacaatcaa
     7321 atcaacttta tctttactct tgatctcatc aatcgatagt tctgcagaaa tcattattgt
     7381 atactcaata ccagcggtcc attgtacgtc ttttaaataa acttccccaa taactttttc
     7441 tgaacttcca ttaccatccc aaccatatgt tagaacagca tgtacatcac tagcattcac
     7501 gcttttgttt ggtttgactg gaatagtaat gacttgttgt gtcatgtcat aaacaatccg
     7561 atcagttttc acagtaaatg aaagattctc atttgcccgt tcatttgcag aagtactgat
     7621 tccaatcatt gctgcaatta tactcagccc aagaataaca aataatacgg tcgctgtttt
     7681 cttcatttct atcactctcc atataattac cttctatttg ataaaggaat tatataaaat
     7741 gaaaatgatg agaacacgac agtcagttgt gaaaatctaa caaaagatgt cgtgtattcg
     7801 cttgaatttg tttacttttt cgtttaatca atgacagact taaaaaagca agaccgatat
     7861 cagccttgct ttcttcatct tttattgata aacagcagtg tatttcttca tccgctttgt
     7921 tttatatgca tagagtaaca aaccaacaat caagactaac aagaataatg tagttgatat
     7981 agatgcttca gccccaaagt ttcccccagt aattattgta ttttgcgatt ggatcagagt
     8041 ggagaaaaaa ccttgttctt gcgttccgct gactggtata cccaaaatag ctccttgtgt
     8101 ccaattccaa gtaatatgag caccaatagc cgtaaataaa cttcctgtac tgtagataag
     8161 tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact gccagaaatg tcatgttagg
     8221 attcaatccg tgcaaaagag caaataatgc agcagatcca atcattgcta ttaacggacc
     8281 aaatctaagc atctgatgct gaaaaaagcc acggaataaa agctcttctg atgcccccac
     8341 tgcaataaat aaaataaaac tcgttatcag cggcacaatg tttgcgtttc ctgaatctaa
     8401 ccgatacgcc cccgtcaaat ataatggact agtaataagc agaaacactc caattccaac
     8461 aacgagacct cccgctaaat acccccagtc ttttcctcga acagaaacac cataatcttc
     8521 tattcttttt ccttcgacct tactagcaag aaataaagct agtaaaaaaa cagctaaatg
     8581 tccgaccgta ccggcaattg ctccaatcca ttcactattt ccaataaaca tatccgcaag
     8641 tgcattattg acaagaattc ctaaaacaaa accaacaaag actattaaaa cagatccaat
     8701 caaccgaata atcctgatca tctttcatcc ctcctttata ttaagtttaa caaccctttt
     8761 ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca tagagagaat agatttttac
     8821 gaactaagaa tatctacagc aaccttacga aaatgaaaga ctgtctccct aattcttctg
     8881 ttagactgtt tttaataagg aaaggatggg aaattttttg aaaaaacaac tactaattat
     8941 gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca atgaaacgat
     9001 tgcaacgttt aaaggtggaa cacttacagt tcaggatttt tatgataacc caaaagttaa
     9061 atcaaataat caaacactat taaaaaaaat gattgtcttc aaagcatttg atgatgtata
     9121 cggaaaagag atttcttcaa aggaagtaac aaaagaatat aatgaacaaa tcaaaaaact
     9181 cggtccaaac tacaaggaac aattaaaagc agttggccaa acagaagaga catataagaa
     9241 actctttaaa cagatgctgg ctttccaata cggtttgaaa gccaatgtaa aattaacaga
     9301 taaagatcta gacactgctt ggaaagaatt ctatccagaa gttagtacac agatcattct
     9361 attttcgact aaagaagaag cagataaagc aaaaaaagaa gcaaatgaag gagaaaattt
     9421 ctccaagctt gttcaagctt atggaaaaaa taaaacatta aaggaaactg atggaaaaat
     9481 gaattttgat tcaaccaatc cagagattcc tactgaagta aaaaaagcag catttaaact
     9541 aaaaaacggg gaagtttctg atatcattcc tgtaacagat ccaacgactt atcagcaaag
     9601 ctattatcta gtaaaaatgg taaaaaaaca ggataaaggt tctaataaag acaaatacaa
     9661 atctgagcta gaaaaaattg ctaccgaagc aaaaactctt gatacggaat tcatggataa
     9721 aacaatccgt aaagtgatga aaaaagacaa tgtaactatt aaagatcctt atgtaaaaaa
     9781 tatattcaaa taaacaaatt tgacccaatt ggggatgtgg caaaatttga gaagtgattt
     9841 ttggaaattc cttcttattt ttgaggcaaa ccacttttgc cacaacttca tcggggcctt
     9901 tttttatttg taccaaaaca aatcactgga tttgttacaa tcatgaggat ataatttaaa
     9961 aaagtttttt atttctatca catgaatcaa tgcgccatta agtagattct ttcatgcttc
    10021 cctatttttt cacttgccgt atcgcttcaa aaacaactat cataaactca aactcgactg
    10081 aaaagttatt ttaaaaaaga ctaaaaatat cccttctgga cttgttcttc ttattctgtt
    10141 atactagaga aacacgaaag atatttttat gcgacacttt tcatttgttt ttatgaaatt
    10201 tattcaaaac tggcgtaact tcagagttac aaggatgtat tagaggttag ggaatacatc
    10261 gtttaaatga gaaataagta atagttagta atagtcaccc aaactttaaa ttaatgaata
    10321 ttgaatcgtc agatagcctc ctcgagtttt cgagaaggct atcgtttatt ttatggagaa
    10381 attgattcag aagtaatcag cgaccttttt tagttcacca atcgtttctg cttggaaagt
    10441 ttgcacccaa tcttcttgga agcccctcac tgctttatca atcgagggca tctctaatac
    10501 ttgatctagt agctctttac ctaacgttat cgctgagctc cagatcgaat ggctgtagtg
    10561 acttgttcac aatttttgaa actagctgct aatatttttg ttggtctttg cgtacgttcg
    10621 atctcagtcc gcaacgaatc aatcactttc atagcatcaa tgccttgatt ttgcatcctg
    10681 ttataataag gtgcaatata atctgctcca ttattaatag ctaaataccc ttgtaattct
    10741 gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt tcaacttttt gatcgcttga
    10801 aggcctgctt gattcaccgg caccttgata aacgtatctt ttcccagttc ttctatgata
    10861 actcgagcat cctctatcat ttcttcttcc gttcttccta ttgtctgaat atggatagga
    10921 atctgtccga ttattttttt gatttctttc atatgcgaga agaagtttga cgctccttct
    10981 tttttcagaa ttgtaggatt tgttgttacg cctgttaaat tcaaaaactg ttgacaagaa
    11041 taaatacttt ttgtatttgc tgtatcaagc atgatttcca tatagctttc ccccattctg
    11101 atcctcttga tcgattttaa tgattgtccc ctttcttaaa tgcggcattt ccttatcttc
    11161 tacatacatt gttcctggaa gttccggctc atgagatcca tcaaatttta tcgtgatatg
    11221 tcctaattca ttcaaattag tttggacaac atctcctact gctgtgatta catacatttg
    11281 tttatcaaaa cagagcttca tatcacaagt tatttctttt cttacctgat tcaattcaat
    11341 cttataacaa taatctgtta atgtttccgg tgcttcatct ccaaataaaa tcattatttt
    11401 ttctttcctg aatacttctg cttctatacc aacatctgtt acaaccgttt caaagatcgt
    11461 catctct
//
LOCUS       DAXWLF010000089        11123 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXWLF010000089 DAXWLF010000000
VERSION     DAXWLF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.089"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            85..1344
                     /gene="lysA"
                     /locus_tag="ACGE4U_001726"
     CDS             85..1344
                     /gene="lysA"
                     /locus_tag="ACGE4U_001726"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFF8393889.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1331..2131
                     /gene="dapF"
                     /locus_tag="ACGE4U_001727"
     CDS             1331..2131
                     /gene="dapF"
                     /locus_tag="ACGE4U_001727"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFF8393890.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2134..3015
                     /gene="dapA"
                     /locus_tag="ACGE4U_001728"
     CDS             2134..3015
                     /gene="dapA"
                     /locus_tag="ACGE4U_001728"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFF8393891.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3012..3728
                     /gene="dapB"
                     /locus_tag="ACGE4U_001729"
     CDS             3012..3728
                     /gene="dapB"
                     /locus_tag="ACGE4U_001729"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFF8393892.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3734..4429
                     /gene="dapD"
                     /locus_tag="ACGE4U_001730"
     CDS             3734..4429
                     /gene="dapD"
                     /locus_tag="ACGE4U_001730"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFF8393893.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4445..5446
                     /locus_tag="ACGE4U_001731"
     CDS             4445..5446
                     /locus_tag="ACGE4U_001731"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFF8393894.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5443..6804
                     /locus_tag="ACGE4U_001732"
     CDS             5443..6804
                     /locus_tag="ACGE4U_001732"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFF8393895.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6764..7915
                     /locus_tag="ACGE4U_001733"
     CDS             6764..7915
                     /locus_tag="ACGE4U_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8393896.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7890..9059
                     /locus_tag="ACGE4U_001734"
     CDS             7890..9059
                     /locus_tag="ACGE4U_001734"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8393897.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9234..10613
                     /locus_tag="ACGE4U_001735"
     CDS             9234..10613
                     /locus_tag="ACGE4U_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8393898.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10894..11123
                     /locus_tag="ACGE4U_001736"
                     /pseudo
     CDS             10894..11123
                     /locus_tag="ACGE4U_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3703 a         1944 c         2492 g         2984 t
ORIGIN      
        1 ttaatattat tccaataaaa ttcaaatctt tatcaaatga aattccaata aaaacggaaa
       61 aaaagtgaaa agaggaatga aaaaatgaca gaattgataa ttggcggagt cacagcaagc
      121 acattggcag aaaagtataa gacacctctg tatgtgtatg atgaagaaaa gatgagacaa
      181 acgatgctgt cattcaagcg aggctttgta tcgaaacgtt ttgatacaaa agtgctttac
      241 gcctctaaag cattccaaac gattgagatg ctgcatttag ttcgtgaata tggattcggg
      301 ctagatgttg tcagtggtgg ggaaatctat accgctttgc aagctgattt tcctagagat
      361 actatctact tccatggtaa caacaagacg ccagaggaac tgatctatgc acttgaaaat
      421 gatttgttac atattgttgc cgataatttg atggaagttg aattattagc agaattaagc
      481 caacgctatt taaaaaaaat ttgtatcatg cttcggttga atgtcggagt agaagcacat
      541 actcatgaat atatcgtcac tgcgcatatt gattcaaaat tcggcatgag ctatgaaagt
      601 aaagagtgcc aacaagccct tcagttgata aaagaaagca gctatctaga gttagaagga
      661 tttcatgctc atatcgggtc acaaattttt gatgttactg cttggttagc tgaaatcgac
      721 aaattgacga attacttaac cgattttgac caacctcttt ccttgaatct tggcggaggg
      781 tttgggattc gttatacaca aaatgatcag ccgatcccag tcgaagaggc actaaaaaaa
      841 ctgattgaat atacggaaga agctttggaa aaacgaaaac tgaccattcg tcaattactg
      901 atcgaaccgg gtagaagtat tgttggagaa gctggtacaa cactatacac gattggtttc
      961 atcaaaaaga caccacataa aaaatattac tttgtagatg gcgggatgac agataatatc
     1021 cgtccagctc tgtatcaggc agaatacgat tgtgatttag cagcaaaact gtcggaagaa
     1081 aaaacagaga aagtgacgat tgcaggaaaa atgtgtgaat caggcgatgt ggtaattaaa
     1141 gaagcttatt tgccgaaagc agaatcgggt gatcttttag ctgtttattc cacgggagct
     1201 tatggatact cgatgagcag caactacaat cgagcgacac gaccagcagt tgtttttgtc
     1261 aataatggcc aatcaagact gattgttcgc agacaagcat tttctgatct actaaggggg
     1321 gaagtttcct atgatccttc ataaatataa tggctgcgga aatgatttta tcttattaga
     1381 ttacgcagaa gacacagatt atagtcaatt agccgcttct ttatgcacaa aagaaaaata
     1441 tgatatagac ggattgatcg ccgtaaaaat cgatccgttg gaaatgatct attacaacaa
     1501 agatggcagc cgcgcgccga tgtgcggaaa tggcattcgc tgttttgctc gatacgtaaa
     1561 tgatcagaac atgatgaatg aacaggaatt caacgtagag acattagcag gcacaagaaa
     1621 aattacggtg ttggatccct caccttttta ttgcagcgta gatatgggag tgccagatga
     1681 ttctccggaa aaaatcaaag ctaatcaaac ttatccaata aaaaaccagg aaatagagat
     1741 caatggtgaa aaagtaacga tcactagttt atttttagga acgattcata ccgttgtttt
     1801 tgttgaaaat gcgcgtgagg aagtctggag aaaaagaggt gaactgatct gtcatcatcc
     1861 tttgttcaaa gaaaaaacca atgtcaactt tgtagaagta gtcaatgaac aagaattgat
     1921 cgtccgtacc tatgaaagag gagtaggttg gacacttgct tgcggtacag gttgctgtgc
     1981 ctcttatgtg gttgctaagg aaactgggaa aatttcagca gatcaggtag ttgttcactt
     2041 ggaacaagga gacttgatga tctctggtga ggatgcgata caaatggctg gcccagcagt
     2101 tcatgaatgg acaagaatat tggaggaatg aagatgataa aaggttcgat cgtggcgctg
     2161 atcacgccga tgaatgaaga gggatcagta gattatgctg gtttggaaaa attgatccaa
     2221 ttccatttag atgaacaaac agatggatta ttagttttag gtacaacagg agaatcttct
     2281 acgctcacac aatctgagga ggagcagatc cttcaactga cagtaaagaa agtggctggc
     2341 cgtgtgccgg tgattgcagg agctggaacg aataatacga aagaaacgat tgaaaaagcg
     2401 aagcattttg cttctttggg ggcagacgca cttttggtca tcacccctta ctacaacaaa
     2461 acaagtgatg caggactagc tgctcatttt acagcaattg cagaagcctc accgattccg
     2521 cttattttgt ataatgttcc ttctagaaca ggcatgtcga ttccaatcca tgtattagta
     2581 aatttagcag aacatcctaa tatcattggt ttgaaagaag cctcaggaga tatggcttat
     2641 gtgatggatg ccgctcggtt gattggagaa gaattcttcc tttacagtgg aaatgatgat
     2701 ttgatcctac cagtaatgag tgtaggagga agcggtgtaa taagtgtatg ggcgaatatc
     2761 caacctaaaa tagtccatga attagtaaaa gatacgcagg acggaagatg gcagcaagca
     2821 aaagaaaagc aattaaatgc acttgaactg atccatgcct tgttcagtga gaccaatcct
     2881 attcctgtca aagcagccat gtcattatta gacttacctt ccggacctct acgcctgcct
     2941 ttagtttctc tttcaaaaga gaaaaagaaa caattagctc agcttttgtt aaaagaaagg
     3001 acggttgaaa aatgaaaatc ggactgattg gcagtggaca gatgggaaat agagtcgaag
     3061 aagttgcaca agaaagagga gatacggtcc ttcttttacg gacggcacct aaagaagcaa
     3121 ctacgcttgc ttttacggaa ctgccagaac tgcttattga tttttctcat ccagataatt
     3181 tagagaaaat cctttcgtat agcttatcat atcaacttcc gttagtcatt ggcacgacag
     3241 gttatacaca atcacagttc caagaaataa aagaacatgc caaacatgta cctgtgatgt
     3301 atagtgctaa tttttcgttc ggcatcatgg tgatgaatca actaatcaag caagcagctg
     3361 caatgttgca aggctggcag atcgaactga ttgaaaaaca tcatagtcgt aaaaaagatg
     3421 caccatctgg tactgcaaat atgctgcttc aaacaatcca agaagtgcaa aagctgcagc
     3481 ccgtatacaa ctggcaaggg aaaaaaagag aagaaaacca aattggtgta cacagtattc
     3541 gtgcaggttc tttacctggt gaacatgatg tattgtttgc tgctactgat gaaatattta
     3601 cgatcaaaca tgaatctttt tctaatcgaa tctttgctgc aggcgcagta gaagcagcgg
     3661 actggttgaa agatcagtca ccaggttatt acaaattaga agatttatta atggacaaag
     3721 gggtgtaaaa aacgtggaag ctcaagaaat catccgctac attcaaacgg catctaaaaa
     3781 gacgcctgtg aaagtctatc tgaatgtaac cgagccaatc gaatttaaag aaagtaaagt
     3841 ttttggtgaa ggaagcagct tcgttgtttt tggtgactat gagacgattg ctccggtttt
     3901 agaagctgaa tcagaaaaaa tcattgaaat cgaaatcgaa actgctgcaa gatacagcgc
     3961 agttcctttg ctagatatta aaaaaattaa tgctcgtatc gaaccaggtg ctattatcag
     4021 agatcaagtg tcaatcggca acaatgccgt gatcatgatg ggagcgatta tcaatattgg
     4081 tgcagtaatc ggtgaaaaca cgatgattga tatgggtgct gtcttaggtg gacgagcgac
     4141 agtgggcaaa aactgccata ttggtgcagg agctgtctta gcaggtgtga tcgaaccagc
     4201 atcggccaaa ccggttatcg tagaagacgg cgtactggta ggtgcgaatg cagtgattgt
     4261 tgaaggtgtc catatcggaa aagatgcagt cgtggcagca ggagcagtgg ttttagaaga
     4321 tgtggcagca gaaactgttg tcggtggaat acctgcacgt gtattaaaaa taagtgatga
     4381 tcagacaaaa gaaaatacag cattgattgc tgcgttacgc gaattataga ggagatggag
     4441 aaaaatgcgc gaaatagata cctgtattgt gggggcaacc ggattagtcg gccaaacgat
     4501 gctcaaagtg ttagaagaat atgattttcc agtaggacga ttgaaattat tagcttcaaa
     4561 acgttcagcc ggtaaagttt gtgtattcaa gaaaaagaaa cacactgtac aggaattgac
     4621 agaaacttca tttgaaggat atgatttagc gctattttct gcaggtgctt ctgtatccaa
     4681 agagtttgcg ccaatcgcac gaagtcaggg actatttgtc atagataatt cctcagcatg
     4741 gcgggaagat tcggatattc cattaatcgt tccagaggtc aatctggaag actatacatt
     4801 gaatcggctg attgcaaatc caaattgttc aaccattcaa gcggtacttc cgcttaaagc
     4861 tttacaagaa gcctttggta tccagcgagt caattattcg acctatcaag cagtttctgg
     4921 ttccgggcaa aaaggcattg aagatcttca tcgtacgcaa aatgacgagg aaccagtttt
     4981 gtatccttat aatattagcc aaacggtgat tccggaaatc gatcagcctt tggaaaatgg
     5041 gtatacaaaa gaagaacaga aaatgataaa tgagacccgt aagattctgc atcagcctgc
     5101 tttgccagta tcagcaacct gcgtacgtgt gccaatcgaa aatggacatg gagtctctgt
     5161 ggcagttcaa ctgcaaaaag agtttacgac ggaagaaatt agagagtgtc tcagccagtt
     5221 tccagggatt caattagaag atgatctagc agcacataaa tatccgacga gcattcttgc
     5281 tagaggaaca gatatggttt atgttgggag gatcagaaaa gacacttctt tagagaacgg
     5341 attgttgttc tatacgactg ccgataacat acgcaaagga gccgctgcga atgccgtaca
     5401 gattgctgca gctttaatgc agaaaatagc ggaggtggca agatgagtaa tacgctgaaa
     5461 gtagcgaaat tcggcggaac atcgatgtct catgaagagc aattcaaaaa agtccgtaca
     5521 attgttcttt cagatcccaa tcgccaagtg atcgttgttt ctgctttagg caaacgtacg
     5581 tcagatgatg acaaagtaac agacttactt tatttgattt atgcttacct tcagcatcat
     5641 gttgattgga cacccctttg gaaaaggatt tgtgatcgtt tcattcaagt aagagatgtt
     5701 cttgccctgc agttacctat tgaagatgaa ctagcacgca tacagaaaca actggaagac
     5761 gaaaagataa ctgaagatta tttagtaagt agaggtgaat atctgactgc tcaactgatg
     5821 gctgagtatc ttggttacca gtttattgac gcaaaagacc tgattttctt tgactatgat
     5881 gggaaaatcg atcaggaaaa aacagcacag gaaatcacaa aagcatttaa aagatatccg
     5941 aaaatggttg ttccagggtt ttacggggcg aaccctgcag gaaaagaaaa attattgggc
     6001 cgtgggggaa gcgacattac cggcgcgata ctggcgaaga tgttgcaagc ggaaagttat
     6061 gagaattgga cggatgtttc aggaatcatg atggctgatc cgcgaatcat cgatcatcct
     6121 aaaatcatcg aggaaatctc ttttagagaa ctgagagaga tggcttatat gggggctaac
     6181 gttcttcatg aagcagctgt tttcccagtt caagaggctg atatccccat ccaaataaaa
     6241 aataccaatg atcctttcgc tcaagggaca aaaatatcga atcacgaaat agaaaaggaa
     6301 aatggactga caggaatagc tggacgcaag gattttcttt ccatcacttt attcaaacgt
     6361 catatgtccg atgaaatcgg gtttatctgg aaagcaatga gcatttttgc taaacatgag
     6421 atatcaatcg aacatattcc ttcagggatc gacaatatcg gagtgatcgt ctcagcagaa
     6481 gcgattgccg ataagttatt tctcatcacg aaagaattaa aagaaacact tggcgtggaa
     6541 gaaatcgtcg tgatcgaaga cttagctttg atctctgtcg ttgggggtcc gcataaagaa
     6601 ttgattggat tatctgggaa agtgttatcg atattgaaca aattagaaat tcgcacatcg
     6661 atcttatcac aaggagcaca ggagttgaat ctgatcatcg gtgtaccgaa taatcaatat
     6721 gaaacagtag tgaagggtat ctatgaaggg atggtgaata cggatgctga aagaatatcg
     6781 aatggcactc catcaaattc ctgaaatcgg gtttcatgaa ttcaagacaa aaaaatattt
     6841 atatgaacaa gtaaaaaaca gcgaggccgt tatccatgaa atcggcgaaa caggactact
     6901 cctttatttt gataatcagc aagaagaaac gattgctttt cgaacagata tcgatgcact
     6961 gccagtcaaa gaagaaacga atctctcttt tgcttctaag catgaaggtt atatgcatgc
     7021 atgtggtcat gacggacata tggcgatgct tttagggttg actgacttta taactaaaca
     7081 cattgaggat cttatttata atgtagtact gatctatcag ccatcagaag aaatttccgg
     7141 tggcgcacaa agcgttattc agtcaggatt gttggataaa tatgaagtac aagcaatatt
     7201 tggctttcat ctatggcctg gactagctaa gggagaagtt ttctctcgac caggaccttt
     7261 gatggcacaa agcagtgaaa cagacattat cattaaaggc cagtctgcac atattgcttc
     7321 tagtgaaaaa ggaatcgata gtctggaggc agctgtacga tttttgaaga atgtttatga
     7381 ttttgaccgg caattaccgg cagagtggaa tcatctaatg aaatttggcc aaatccaagg
     7441 tggaacgatc cgaaatgttt tagctggaga agttattgta tccggaagta tccgttctta
     7501 cagttcagat atgcaaaatt atttgaagag gggattagac catttagcag aacagtttca
     7561 ctctacagct actatttctt ttcgctataa tgacggctat ccagctgtaa tcaaccatag
     7621 aggcttatat gcagcactgt tagattcaaa caaactacat gaattagatg ctccagttct
     7681 tcaagccgaa gattttggca tatatacgca acaatatcca tgtgtatttt tctttttagg
     7741 tgtcggtgat acccctgctt tacatgatcc gacgttcgat tttgatatgg atgttttaga
     7801 aaatggtgta gaatggtaca aaacgattct ttttacagaa aatcttcttg taaaaatcga
     7861 tagcagttca aaagaggagc gagtaaataa tgatgaattt atccaatcga tttagtccta
     7921 ggctttctag aatagaagta tcaaaaatac gacaattcga tcagcaaata tcgtcgatac
     7981 cagatgtgat caaactcacc ctgggagaac cggattttcc aactcctgaa catgtcaagc
     8041 aagcaggaat agcagcgatt gaagaagact tttctcacta taccggaatg agaggattag
     8101 aggaactgcg ggaagcggct tgtatatttc agcaacagcg ttatggatta acttatgatc
     8161 cgcaaacgga agttttgacg acagttggtg caacggaagc gattgcctct gcgttgttat
     8221 ctgttttgga agaaggagac aaggttctta ttccagcgcc tgcttattca ggatatcagc
     8281 cattagtgga actagctggt gctgaattga tcccgataga tacttctgac acagggtttg
     8341 tctgtcagcc ggaacaattt gaacgagcat ttgaacaata tggatcagct gtcaaagcag
     8401 tcattttaaa ctatcctaac aatcctactg ggacgacatt gtctgcaaaa caattgaaag
     8461 caatcgctga ggttttaaaa aaatatccgg tatttgttat aagtgatgaa gtatatgctg
     8521 aattgactta tagtggaacg catatgtcca tagctactta tcttccagaa caaacaatcg
     8581 tgataagcgg gttatccaag tcacatgcga tgactggttg gcgagtgggc tttatttttg
     8641 ctcaaaaagc ccttattgac gaactaatta aagtccatca atatctggtg actgccgcta
     8701 caacgatgtc gcaaaaagct gcggctgaag cgctaataaa tggggtgaaa gatagcgaga
     8761 atatgaaaga gcaatatcaa acacgaagag actatctggt ccagcaattg gcgcctttag
     8821 gatttgaagt gacccagcca aacggtgcat tttacttgtt ttgcaaactc ccagctgcca
     8881 ttcaaactga ctcttggcag ttttgtgtag aattagcgga acaggcaaaa gttgcgtgta
     8941 tcccaggaat tgcatttggc ccagaaggag aaggttatat ccggatttct tatgctagca
     9001 gcatggaaaa attacatgag gcctgcaaac ggatcaaaga ctttttaaca gaacagtgat
     9061 tcatagattt tatcaaaaaa acaatcgtta attttgcggt atattctgca aggagacgat
     9121 tgttttttaa atttagttca aatgaacgcg tttcttttga ttcataacta atgtaatgtt
     9181 aagtttaaat tgtttagaat gattttaaaa gaatattcaa ggaggtttca tcaatggaaa
     9241 aaatcaaagt tgtcattgta ggtgcatctc atggagggca tcaatcgatc ttggaactat
     9301 tgacacgata cggtgaaaat gtagatatca cgctatttga agcaggagac tatatttcct
     9361 tcatgtcatg tggtatggag ctgtatttag aagacaaggt aacagatgta aatgatgtac
     9421 gtaatttcag accagaaaac tttccgcaat ccaatgttca tattttaaat aatcacgaag
     9481 taaaaacgat cgatgccgat aaaaaaacag taacggttac tagcgcatca gataacgaaa
     9541 caaaagaata tccttatgac aaattgattt tgagttctgg tgtcaaacca aacaatctgc
     9601 cagtaccagg agcagatcta gaaaatgtct acttgatgcg tggatacaat tgggcaacca
     9661 aaatcaaaga aaaattaaat gatccggcta ttaaaaaaat cgctgtcgta ggtgcgggtt
     9721 acattgggat cgaagcagca gaagcaagcc gaaaagctgg gaaagaagtc gtattgcttg
     9781 atgtgatcga tagaccttta ggcacttatt tagatgctga gatgacagat attttagaac
     9841 aacacctaaa tgaaaacggt attgaagttt tgacaaatgc aaaaatcgaa gcttttactg
     9901 gtaacggaaa agttgaggca atcaaaacaa gtgaaaaaga aatcccagca gatctagtca
     9961 ttcaagctgc cggtgtgaag gcaaatacag aatggttaaa agggatcgtc gacttagatg
    10021 aacgtggctg gatccaaaca aatgaatact tgcaaacgaa tcttccagat gtgtatgcag
    10081 taggagatgc tacacttgct tattctatcc cagcacgcaa aaaaatgccg atcgctttag
    10141 cgactgttgc acgtagagaa gcacgttatg tagtaaaaca tctttttgaa gaagtaccaa
    10201 acaaaccgtt cggcggagtc gtaggttcct ctgcattaag tgtctttgac tatcattttg
    10261 cagcaagcgg attgaatagc tttacagcag caaaagcagg agtagatgta cgagtgtctt
    10321 attatgaaga tacgttacga ccaaaatatg taccagaaga acaaggaaat ccaaaagtgt
    10381 ccatccaatt attctcggat ccaatgaccc atcaattgct aggaggagca gtattatcta
    10441 cctatgatgt tacaggtcaa ggaaatgtat tggcactagc gatccaaaac aaactgactt
    10501 tagaagaact agcagaagct gacttcttct tccagccagg ctttgaccga caatggagca
    10561 tgttgaatct agccgctcaa caagcgttag gagaagaacc atttgtagaa taagaaaacg
    10621 aaatatttga aaagaggttg tgaaaaagct gccttgcatc aagtaataag aacagccaag
    10681 taaaaacagc ttcttatgtt tttaacttgg ctggacttat taccgcagat gcaagctttt
    10741 gaacaccgtt tattttcaaa aaagggactg tgacacgatt tgtgtaccga cccccaaaag
    10801 ttagaccaaa aaatctaact tttggaggtc ggtatttttt atggttaaat atacttttga
    10861 acttaaatta aagatcgttc atgattattt ggatggcaaa ggcgattgga ccgtaaaaat
    10921 ccagatcaag agattgaagc ggaaatgctt aaaattcggg agaagcacaa ggattatggc
    10981 tgtttacgga tgaccaatga actaagaaac cgcggtttct ctatcaataa aaagaaagtt
    11041 cagcgcttga tcaagaagct aggaataaaa gtaacggcat acacgcgtaa atcacgtcgc
    11101 tacaattctt atcgaggaaa agt
//
LOCUS       DAXWLF010000090        11114 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXWLF010000090 DAXWLF010000000
VERSION     DAXWLF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.090"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            81..950
                     /locus_tag="ACGE4U_001737"
     CDS             81..950
                     /locus_tag="ACGE4U_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFF8393899.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            970..1317
                     /locus_tag="ACGE4U_001738"
     CDS             970..1317
                     /locus_tag="ACGE4U_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393900.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1330..2592
                     /locus_tag="ACGE4U_001739"
     CDS             1330..2592
                     /locus_tag="ACGE4U_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393901.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2672..4048
                     /locus_tag="ACGE4U_001740"
     CDS             2672..4048
                     /locus_tag="ACGE4U_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8393902.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            4045..4674
                     /locus_tag="ACGE4U_001741"
     CDS             4045..4674
                     /locus_tag="ACGE4U_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393903.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            4744..5172
                     /locus_tag="ACGE4U_001742"
     CDS             4744..5172
                     /locus_tag="ACGE4U_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393904.1"
                     /translation="MGETIEYKDKNYIIIGIYNTRINRIGDPRITSDLVCQSVNYSPD
                     LTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNNEEKIFYQIYGIEKFEYE
                     HVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            5731..6330
                     /locus_tag="ACGE4U_001743"
     CDS             5731..6330
                     /locus_tag="ACGE4U_001743"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFF8393905.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            6346..6822
                     /locus_tag="ACGE4U_001744"
     CDS             6346..6822
                     /locus_tag="ACGE4U_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393906.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            6839..7741
                     /locus_tag="ACGE4U_001745"
     CDS             6839..7741
                     /locus_tag="ACGE4U_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393907.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            7814..8005
                     /locus_tag="ACGE4U_001746"
     CDS             7814..8005
                     /locus_tag="ACGE4U_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393908.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            8169..8756
                     /locus_tag="ACGE4U_001747"
     CDS             8169..8756
                     /locus_tag="ACGE4U_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393909.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            8756..9655
                     /locus_tag="ACGE4U_001748"
     CDS             8756..9655
                     /locus_tag="ACGE4U_001748"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFF8393910.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            9655..10569
                     /locus_tag="ACGE4U_001749"
     CDS             9655..10569
                     /locus_tag="ACGE4U_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393911.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            10621..10836
                     /locus_tag="ACGE4U_001750"
     CDS             10621..10836
                     /locus_tag="ACGE4U_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393912.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         4269 a         1501 c         2255 g         3089 t
ORIGIN      
        1 aaaatacatt tgacaaaatt tacaatatag tttattatat atttatctta ttatgataac
       61 acaataaaag gaatggtggt atgaaaaaga agaagtttag ccgatcgttg ttaggaattt
      121 tggttttagt tgcggttggt cttagtggca agggttactt tcagaataac agtactcagg
      181 aacatgattt tcagatcaaa agtagtgttc aggtaggtaa taaagcagac agtgaatcaa
      241 aagaattttt agggatacgt tatgatccaa acaaatatcc aaataatatc gtaaacatta
      301 atgatgggaa atatacactg acaaaagaag accatgaagc attatcagaa aaaggcagtc
      361 aaaccttttg ggtaaattat gcagatttag atcaacttgg aagagcagga gatgttgaag
      421 cgttgatcac ctatgatgct gtaaattctc actcatctaa aaatgtggaa cgtccatctt
      481 tcaaaagttc tgtccatatt gcaggagaat acaaggatgg agtatttgat caagagagag
      541 atacttgggt cggcaaagat tcaaacaatg aaatcactca gctagaaggc tataaaggtt
      601 atctctataa caaaagtcat tcattagcat ggtcgctagg aggagatatg gaggtacaca
      661 atttaacact tggaacgaga gcgcaaaatg ttgggaaaaa tgatcagaat ggagggatgg
      721 cttactcaga aacgattgta cgtgatgcaa tctataaaaa taagaatgaa aaagtattgt
      781 ataaagttga tccaatttat aaagggaatg aattagttcc tcgtggttct catgtcgaag
      841 ctttctcagt taacgataat ggaaaaacgg taaaccttaa tgtttatgta tttaacaatc
      901 aaaaaggaat caacatcaac tatcaaaatg gtacttgggt aagagattag aaattgagaa
      961 ggtgaagaat tggaaacaat aagcatcaat ggattagata aatcagttgc aaatcgaata
     1021 aaaaaaggaa atggctacta tcttctgaag aagggtaaat attcagttgc tagagagtta
     1081 gaaacaatga gtatcgtgta tgtagctaag aatcattttg acgtagaaca ggctattttt
     1141 gatccagatt ctgaaatagg gaaatgtgtt gaattatcag agaaaactgt tagtacaaaa
     1201 ggggcgatga tcattgagag agaaatgtta gacgaaatca gagtaatcat tcctaaagat
     1261 acgcagatgt gtgaggacag cataagtgac gatgaaccaa aatcagaaag aaaataagga
     1321 gatattcaga tgagcgaaaa atccattgaa ttaaaggaat ataagcgaag cgatggagaa
     1381 aaatattatc ctgtagccag tttttctaaa tcagtatatc tggttggacg tgcattggta
     1441 gttggtgcaa ttggcatata tattttgcat tgggtaacaa aggttgtaca actgcttgta
     1501 gataccttag taagtattct tgtctcgtta aatgatgtta acggaacaga tgatttagga
     1561 ttaaaagaga tgtttaaaga gttactcaat gcgttttttc ccaattattt aagttattca
     1621 tataatgtac aaagcttttt tgcaaagcta cacttggtag acatctctat tttagtatta
     1681 gaagcaggaa cggtattctt tgttggatat ttggttactt tgatgttcag gcatcactat
     1741 ggagaacaaa gaccattttt tgatgatatg gaaagtagaa ggttgaaaag agaagctcta
     1801 gaagctatgg atgtgaagta tgatccaaac aagattatag gagatgaaac gaacaacaaa
     1861 aaaagaaaac gaaacaaaac gcaattagaa tccgatgtgc tatcaacgct taaaaatatc
     1921 aaaatttctg ttcatacaag acgtgatcta agtgtcaatg aagatattcg agaatatatc
     1981 gtaaaaatcc cgcaaccaaa agggaagaaa gttagagaaa cagttttacg acaaattcaa
     2041 gatttagggt cgatcctgac aagagcaaca agcggacgag ccaaatttgg tgaaaaagaa
     2101 gatgaagttt caggcgatta tttcattttc cgagcatcag taaatattga aagagaacgt
     2161 aaggaaagag taagtaccaa aaacaaagaa acggaaagag caacatcttc aatcgcagtt
     2221 tcaaattcac aattttcttt tccaatcaat ctctttgccg ataattccaa aaatatccaa
     2281 aaccaaaccg aaaaagctag aaaatatgcc gaaactcaaa agcaatcttt agaactttat
     2341 ctatcttctg ctgacgtacc tgcaagtcta acagatatgc acgttgggaa tacatctatt
     2401 gaatatttgt ttactttatc tcgtaccaga aaaattcaac taagctcaat gaaagaagaa
     2461 attgaaactt acttgcatat atcaggtgta atcgtgcgga tcaacgcagg gcgtattcaa
     2521 attactttac cattaggaga aaatgatcgt attccaattg atgtaccatc aatgatcaat
     2581 agcgtgtttt agaagacatc acaaatagaa acagaaaata caaactacca gttcacaaac
     2641 agaaaagtac cgagaaaagt gaggaataga aatggctatg aaattattag agaaagtcaa
     2701 aaagagagcc gaaaaagctg aatctatttt gctagataaa gaacctaata taaagcttaa
     2761 tggaaaattc agtgtcaata tgttgaacac aaaggctaaa gacccaacag atgctatttt
     2821 tggaattaag cctgatggca caattgctca tttttgcatc agcgaaatgc cacacatgtt
     2881 ggttgcagga acgacaggat cagggaaatc agtattactg aatgaaattc ttgttacagt
     2941 aatgtgtcac tcaacgcctg atgaattaaa aataggaatt gtagacccaa aaagggttga
     3001 atttggaaga tacaaaaaat tgccgttcat gttggctgat ccaattacag atatggatga
     3061 agcttatgat ttttttgaat atcttgtgat cttgatgcat gaacgttaca aactgatgga
     3121 aaaagcaggg ttacaaaaca tcacgctata taacgaatat gccgaaaaaa atggattaga
     3181 tagatttccc tatgtgatct tattagttga tgaatattca cagttggttg gaacacataa
     3241 agaagtagaa ggtttgattg ttcaattagg acaaatggca agggcagcag gtattcatgt
     3301 tatcttagca acacaatctc ctcgatcaac agttgtcact ggtattatca aagccaattt
     3361 tccgtcacgt gtttgcctta tggtagcaag tgatttagaa agtaggattg tattagatga
     3421 aggcggtgga gaaagtctaa gtccaaaggg agatatgctt attaagcttg ttgatggaag
     3481 tatggttaga gcgcagggag cttacatcag taacaaagaa attgaaacaa tcttcaatca
     3541 ccttagaaat actatgccag aaccagaata tgttgattac cgagcaatcg tagctgaaac
     3601 tcgtggtgag ggcggtgaag acgaggattt tggtggaatt ggtaaagctc ctgtaagaaa
     3661 atctgctaaa agaaatttga acacaccttt agcaaatcca tcatctaaag agccatcaat
     3721 tgaaaagaag agtgcgtttg ctattaagct tgaaaagaac aaagaagaaa aagtcgagca
     3781 gggagctaaa acactagaaa aactgcaagc agaaaaacta gaaaaagttt taagggcatt
     3841 aggaaaagct gatcctgaac cgcaaacctc aaacaacacg aataataaac agccgattgc
     3901 atctaaaacc aaacctaaag ctaatgatcc aaaaggctca gaaaacgtag tgatcacaac
     3961 tgataaatct gaaaagaaac aaataaatgg aagtacttca cctatcatgc aaaaaatgtt
     4021 aaaagcaaaa aaacaggtgc ataaatgatc atcgtcagtt cttttttaag atggcttact
     4081 ccatataaat atacagatga agtagttgtg aaacgagcga agaaaagaga taaaaaagcg
     4141 gtactaactc ctaaacagat gcttgatcat ggcattttag gggtgaaggt tttagaagtg
     4201 aataaaccgt ttgaatcaaa tagtgcaaga gaaaagttgc ttggagctga agccgatgtc
     4261 gcatattttt accatgacaa aagcaagcat caaatagcta ttaaattcaa tccaaaatcg
     4321 gcatggcggt atcaagcaag agatgacttg aaacgtagga aacttagacc aatgctctat
     4381 ccagaaggaa aagaagcatt tgatagaacc cacatcgttc ccattggttt tcatggtaca
     4441 gaatcagata accgcctatt agtaggttgg aacagcaatc aaaatagagt tcagatcaat
     4501 aaatttgaga agaaagtaag gaaattcaac gatcgaaaaa cgatcctatg gtttgcagat
     4561 atttgtaaac agccggatgg aacagcaaag tgggaagcgg tgatatttga cgaaaatggt
     4621 aaaaaagtaa tgtctgaaac atggcatgat aaagctaaat ttgtttggca gtagcagtag
     4681 aggtgaaaaa gattgctaga actttttgaa gcaaatagaa caggctttaa tcaaaagtta
     4741 gaagtgggag aaactataga atataaagat aaaaactata ttattatcgg aatctataac
     4801 acgagaatta acagaatagg tgatccacgt ataaccagtg acttggtttg tcaatctgtt
     4861 aactattcac ctgatctgac aagcaggtat aagaaatacg tgttattaga atatagacac
     4921 aagattactg atgaaattgg tgtaaacaac acacgaaaca tatttaagat cggaacagtg
     4981 attccttata gtaacaacga agaaaaaata ttttatcaga tttacggaat agaaaagttt
     5041 gaatatgaac atgtcgatct gctagtgacg taccaaatgc gtttaataga accttggtca
     5101 caggcagaaa ttgataaagc agtaaaattg aaccgattat caaaatttaa cgtattagga
     5161 aatgcctttt aaaaatcgca atacacaaac agaaaaatac caaaaaatta gcaatgaacg
     5221 taggtaagaa catgacaata aataataata accctaaaaa atataatgct cttgtccaat
     5281 gctatatcga aaacaaaatt acaatgcaag aagtcgctga tgaattaggc aaatcaaaga
     5341 gcctagtatc acacgatttt aagcacaatg tttttccgaa cccgattgag ctgaggaaaa
     5401 aatttcaaaa gagagtttat caagaaatag gtaagtggat tgatgaaggg aatgcaattg
     5461 accctttaat tgaaaaatat ccttttttaa aaagcaaaaa taaaattcaa aggttaattc
     5521 gtcaaggcga tattcaagct aaagaacctg ttttgacaac caatgatttg gctaaaataa
     5581 atcttcataa acaaattatt gaagactttg aaaaaaatct ttcaacaact gaaattttaa
     5641 aaaaatacaa tattccgaga ggaaagctaa catcgactct ttttagagaa tatggagaat
     5701 cggcagttac taaagcaaag gaagtagcga atgagagaaa aaaacaaaaa tagtagtcga
     5761 tacctagtag cgactgtagt ttttgtttgt gtattgattt tcctgtttga ttttttgcag
     5821 attggaaaaa gcataattgg aaatacattg agtgcaaagg aaatggttgc tttaggtggt
     5881 ttgtatgaag gacacttgtt cccgaatata ttcatttcta tcgtttctca tattaacttg
     5941 taccatttaa tttctaattt attgttctta gtagttcttg aagaagcact gaatgatcag
     6001 tatgagtggt taacattatt gatcgtttta atcatatcag gatttctagg aagcttagtt
     6061 ccttttctgc tacacagcaa catcgtaagt atggggctta gtggaggtat ttttggatgg
     6121 attgctttat ttgcgattgc atacatccgt tttgatagca agaaacattt attactaagt
     6181 gtttttgtat tattgataat cggcatttta ttggtatcta cttttgaatc aggcgaggca
     6241 aatgtcttgt cacatgtaat cggatttgtc tgtggaatga tcttgggatt tattctaaag
     6301 aacgtttcat tgatcaaaca aaagaattaa aagggagtgc taaaaatgcc aaaaagaaaa
     6361 gaaaagaaaa ttccagtgtt tttgtctgtc tcagaaatgt tatcacttca aatactgtta
     6421 agaaaaaaga ttgaaacaat agacgaagaa gttagaaata aaagggctat gtacgaacaa
     6481 cgtgaagcgc taagagaacg aatccactat tttgatgata tacacactaa aatctatgaa
     6541 gcacgtacaa atcacgaaga cgaaagtaaa ttaggattag ctcaacaacg gatacttgaa
     6601 tggcttaata cgaatactga tcaagaaccg ttaagagtga ttagtaatct ttttgaattt
     6661 ggaacgaagc attggtttaa tgctgtagag aaggatgctg acttacaaga agcgtttgat
     6721 acgctaagta aggatagcaa atccatgcga gatttattga ttacttatct aaatgagcat
     6781 gagtttgaac aaacgaaaac caaaaagaaa aataaagaat agaaagttgt gatgaatcat
     6841 gctattaacc ctgttaggtt atttatgttt aattcttgct ttttttgggg caatcgctat
     6901 aattattggt caatttcaaa caaataatcc gattatgcgt aacataggca ttggaattgt
     6961 gattgtaacc tttattgtgt ttaatgccac taagattttt ggaagtaacg ataaacaatt
     7021 tggtatatcg ggagttaata atagttatgc tccagattct ttgggaaggg tagtaataga
     7081 tggaacattt gaaaatgtaa cgagttataa aaatgtttcg ctgacagtga aggaagatgg
     7141 caagaaagca tcagacgacg ttctcgctac tattaagccg tctcaatcac aatttgaagt
     7201 gttttataca gttaaagaga atagccctgt acacaagctg acattggttt ttaacgttga
     7261 tggagaagaa tactataaag atgtaaaaat aaataaaaca tcaacgaaaa agattatcga
     7321 atcagcgaaa aaagaagtag ctacagaaga aagtacaact gaatcaagtt ctactgtcga
     7381 agcggaacta ccagataaaa ttgattttga tcctgaaaaa gtcaaatcgt ttgaatctgt
     7441 ttctggttct atggctgatc ggtttaacac tgaagtcttc aaagctatca aaactgttaa
     7501 attggatact gaaaagcaaa tgattaatgt taccttattc aattcagttg aatctatgga
     7561 tcaggataaa aaagttacgg ttgcaagaga actaggaaaa agagtaattg gtttgtatcg
     7621 tggtactgct aaaccagatt cagacggtgc agatttgcca gttcatgtag tttatgaaga
     7681 cggtaaagtt tttggagatt ctacagcaaa cgaagcatta acgattaaat tggctaaata
     7741 gccagaaagt tataataaac aaatggtgtc acaccaataa tatataggtg tgacaccttg
     7801 gagatgatag ctgatggtag taaataaaga aaaagttaga aagtatgtga ctttaaaaat
     7861 agcttctgtt aaatatttag aaaagcgagc taaagaagaa gaaagagcaa gaggtaagaa
     7921 agtaacagtt tcagagttgt tagaagaatt gatcgagaat gatcaaacaa taagacgtgc
     7981 atttactaat aggtctatca gataacaata taattaataa aaaagaaaga gtgatttggt
     8041 cttagactaa atcactcttt ttatataaag tttctttaca ctcattccga ataatgttaa
     8101 aattctttat atgatatata gttccttgtc agaggaagag ataaccagca atattggagg
     8161 gataataagt ggaaaacaat aatgatatta gacattttag cattggggaa agagttacaa
     8221 cagtaagtga attatcaaag gattatagag ataaagaggg aacgattatt tctcttaata
     8281 agaatcgaag aggcgtttgg caatatgttg ttgatttagg tgatggcgtt acagttgagt
     8341 gtaaagatag tcaactgatc agaaataatg gcagtcattc cattgaaaaa gcaattcttg
     8401 tatacccaaa agtatcaagt aagatcatga atgactggtt gcttggaaaa tttggtgagt
     8461 acaataaaaa agaaatcgca gaagtgatcg gcaaatctta tccaacagta ttagggtatc
     8521 taaaaaagat aacgagtaaa gaagcaaaaa ttgaggatgt tttaacagta agagagcttc
     8581 aatcattaat cgagtatcgg aataaacaaa taacaaatcc tgagtacctt agaaaagaag
     8641 atggtctatt gaaacaattg gaacaagtaa ataaaatccg gaagatgcaa aaggtagctc
     8701 ctttaagcct tgataccttt aaaaaaatct atttgaaaga gatcaaaaaa gactaatgtg
     8761 gactgtaact ttaccgttcc aacctgtatc tgcaccaaga ccaagtgcaa gaaggaatgc
     8821 tgctgatgaa actaatgacg tagcagagaa aaggatttca acctactatg ataaaaagta
     8881 tcttgattat ttgaaaaaaa taaagagctt tatcgaagat caagatttat tagatgatga
     8941 attttactcg attgtaaatg atcctatggg cgcaattatg gaagtggact tttactacca
     9001 gataccaaaa agctataaaa aagtctataa cattatgaag acaacagcgc cagatttaga
     9061 taatttagta aaaagtgcta tggatggtat ttttaacatc tcagctatac gagatagcca
     9121 tatcacaggc ttaattgctt ttaaatataa tgtggcgaac gaaggaaaaa cagttgtgag
     9181 gatcaaacga tattcagaat ttgagtggga tacttctgaa ggattgaacg gagatatatg
     9241 ggaagctaca tttgatttta aaccagtagc aacgccaaga ccaaagtcta aatttagagg
     9301 aaatgggatt atcacttact atccaaaaga gtacaacgac tatttggaaa atatcaagaa
     9361 tacgcttaaa gagaaagatt tggtaaatga tcaactgaaa aaggtaatga atgctcctat
     9421 gggggttata gctcagatag attattttta ccaagtgaga aaagataaga aaaagcttga
     9481 ttcactgatg cgtacttcac agccggatat tgataattta gtcaaaggaa cattggacgg
     9541 aatttttaac aaggaaatag gcatcaaaga tagcagggtt gtagggttaa ttgcttttaa
     9601 atacaatact tttgaaaagt cgaaaacaaa cgtgagacta caggagatgg gctgatgaat
     9661 aataatgaaa aaattcttca tgtacttgat agctttgaaa taattcaaga agaactaaaa
     9721 aaatatcggg atgttttgga acaaagatat gattttgtca atcagcaaaa gagtaatcac
     9781 atggatttca ttctcaatat gaatgacttg aagaagaaat tagttgaacg aaaagaacaa
     9841 gaaaagttga tcaaagctta ctttgaactc ggtgaaaaag aagttaaaaa tgcaatggaa
     9901 ctgaatgaag agcgcagggt tttagatcag ctgttagaac aattattggt gatgtttcaa
     9961 aaaggacgaa tagatgagga tttaattgaa gaaggattga gaaaatatcc ttcaaactca
    10021 ggtatcggaa tagtgctgaa agcgattgat gaagaagaaa ttgaagcttt cataccgcca
    10081 gaggattttg aatcggcaat ggaatatata aaatattata gccaagggat cactgctttt
    10141 agagagtttg atcctgaaga tgttattcat gacttgaaca acctaaaaga atggtgtgaa
    10201 agttatggag tcgatgattc cggattggac tacttgatca gtattatgga aatcgaagaa
    10261 gagatgccgg ataagcctga tccaacagat attttagaat tgatccatga agctagaaat
    10321 ccaattgcct atatttctag aggatatact gtattggaat actataagcc ttatatatca
    10381 gcgatgaacc atttaagacg tgtattaagg gaaaaacgtg aataccgctc agtgttgaat
    10441 gcaacgaata gattagaaaa agctgtttca gagctagatg cttattatcg tgaacattac
    10501 ctgcaagcag gtggtatgcc aagaaatacg aaagcaaata tcagtaggta tataaaaaaa
    10561 gctgagtagg cggagttagc ccataaaatg gcaaaaggta acctgaaagg ggcattttga
    10621 atgaaaaggg atgtttttga attgtacgta ttagaacgtc tagaagacgg catgatggca
    10681 caagagataa ttgatgaaat tgatgatatg atcagcagat ttggtcaatt ggagttgaat
    10741 gatggaaaca aaaattcggt agtgctaaaa caattttggt tgaacaattt ttcgtataag
    10801 gattttatca aagaaaaggc aaatacattg tactgactaa attcacgagg agcagtgatg
    10861 agagcttatt gctaagagca gttaggctct tttttgtttg tgtaaaatta tttgttttat
    10921 aatacacatt tatacacaaa aaaatattga cagatagtac acaaatgtgg taggatatat
    10981 ttgtaagcaa gttagattaa caagttaaag gaagagcagt tatgaaaaaa gaagaactaa
    11041 tagatatatt tcaaattgtt gaacgagcaa ataatatggg aattatgttt tttgatcgta
    11101 tttctttaaa gatg
//
LOCUS       DAXWLF010000091        11041 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXWLF010000091 DAXWLF010000000
VERSION     DAXWLF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.091"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..740
                     /locus_tag="ACGE4U_001751"
     CDS             <1..740
                     /locus_tag="ACGE4U_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8393913.1"
                     /translation="FKFLFSSDQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRN
                     KRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYW
                     PFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLM
                     TILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGL
                     YQSVVGFVLLMITNTIARRFDSDSALF"
     gene            752..1672
                     /locus_tag="ACGE4U_001752"
     CDS             752..1672
                     /locus_tag="ACGE4U_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8393914.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1713..3170
                     /locus_tag="ACGE4U_001753"
     CDS             1713..3170
                     /locus_tag="ACGE4U_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8393915.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3515..4129
                     /locus_tag="ACGE4U_001754"
     CDS             3515..4129
                     /locus_tag="ACGE4U_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFF8393916.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4122..5846
                     /locus_tag="ACGE4U_001755"
     CDS             4122..5846
                     /locus_tag="ACGE4U_001755"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8393917.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5843..7294
                     /locus_tag="ACGE4U_001756"
     CDS             5843..7294
                     /locus_tag="ACGE4U_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8393918.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7767..9920)
                     /locus_tag="ACGE4U_001757"
     CDS             complement(7767..9920)
                     /locus_tag="ACGE4U_001757"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFF8393919.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIRQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9922..10968)
                     /locus_tag="ACGE4U_001758"
     CDS             complement(9922..10968)
                     /locus_tag="ACGE4U_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8393920.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
BASE COUNT         3623 a         1941 c         2172 g         3305 t
ORIGIN      
        1 atttcaaatt tttattttct tcggatcaag catttctcat cactaaaaat acaattttat
       61 ataatgtagc atttatcctt ttgaatctgc tgatctcagt cgtatttgca atcattatga
      121 gcgagttgag aaataaacga ctcgttaaag tttatcaaac gatgtcctta ctaccttatt
      181 ttctatcttg ggtcattatc agttactttg tttacgcttt tttaagtcct gacaaaggaa
      241 tattcaatca gtggatcact ggacatggag gagaagctgt caattggtat aatgaaccga
      301 aatattggcc gtttatcttg gtgttcattg gtacatggaa agggattggt tataacagca
      361 ttatttactt tgcatctgtt atgggaatcg atccgactta ttatgaagct gctatggttg
      421 atggagctag caaatggcag cagatcaaac atgtgacgat accacaactt attccactaa
      481 tgacgatttt aacgatttta gccgtaggaa acattttccg agctgatttt ggcctgtttt
      541 ataacattcc taggaattcc ggtgcattat atgatgtgac tcaagttttg gatacgtata
      601 tctacaatgg cctgacatcc acaggagatt ttggtatgac ggctgctgca ggactttacc
      661 aatcagttgt cggctttgtc ttgttgatga tcacaaatac gatcgcccga cgttttgata
      721 gcgattctgc attattctaa ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca
      781 agtccgctct ttcaataaga cggctaattt tttctctaat ataatgattg cactctttgc
      841 tatttcctgt attttgccat ttatatttgt tatcgccatc tcttttaccg atgagtccat
      901 attgtcgact cacgggtaca gtttttggcc agaaaagttc agtatatttg gttatacgta
      961 cttgttcgac cagatgcagg ataaaatttt ccaagcgctt ttcgtcactg tacttgttac
     1021 tgttttaggt acactgatca atagcacggc tacttcttta tatgcttatg cgatttcacg
     1081 gtcaaacttt ccattccgtc gtttttttac cgttttttgt ttgattacga tgttgttctc
     1141 accagggatg gtggccaatt atcttgttat gacaaatctt cttcagttaa aagatacgat
     1201 ttgggcattg atcttgccaa tggctgtcag tcccttcaat attattgtga tgcgcacatt
     1261 ctttaaacgt tcggtctctg attcgattat tgaatcggca cggatcgatg gagctagcga
     1321 actgcgaata tttgttcaga tcgttttacc gttagctgtt ccaggtattg cgacaatcag
     1381 tttatttgcc gcattgggtt actggaatga ctggttcaat gctttactat acatccaaga
     1441 tgataagcta gttccactac agtatctttt gatgaaaatc caaagtaata ttcaatatct
     1501 gacacaaaat tcaggagcag gaagccaatt gacaggcgga ttagcctcag ttccaggaga
     1561 atctgccaga atggcgatcg tagtcatctc gacgttgcct attgcgatca gctatccatt
     1621 tttccagaag cattttgtca aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa
     1681 aaatataaat cataaaatta ggaggaagaa aaatgaagtt atggaagaaa ctagccttta
     1741 caggtgtttc agcgttgttg ttgggaacat tagcagcatg cggaggttct ggttctaaag
     1801 accaggcaga agctagcaat tcggacactt tgcagatgta tcaaatcgga gataaaccag
     1861 ataatttcga tcaattgatg gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa
     1921 aagtcaatat caattacatt ggctggggcg attacgaaaa gaaaatgaac gtcatcattt
     1981 cttccgggga aaattatgat atcgcctttg cgaacaacta tgtttccaat gcccaaaaag
     2041 gagcctttgc tgatttgact gaattagcgc ctaagtacgc tgaaaaagct tataatgatt
     2101 tagatgaagc atatattaaa ggaaatctgg tcaatggaaa attatatgct ttcccagtca
     2161 atggaaatgt ctttgctcaa caagtactta cttttaacaa agctttacta gataaatacg
     2221 atctttcaat tgatggaatc caatcttacg ctgatgcaga aaaagtcttg aaggaattcc
     2281 atgaaaaaga accaaacacg gcagcttttg ccattggaca aggcttcaaa gtccaaggga
     2341 actttgatta tcctttaggg aatacgttac catttgctat tgatctggat ggagatgatt
     2401 cgaaaatcat caaccaatac gataatgaaa cgtatatcgg actgttacgt acaatgcata
     2461 gctggtacaa acaaggactc attccaagtg acgctgcaac gagcaataag gattatccac
     2521 tagaaggaaa tacttggcta atgcgtggag aaacacaagg accgtatgat tatggcgata
     2581 caattttgac aaatgcagca aaacaagaac ttgtctcaaa agcaattact gttccactaa
     2641 aatctacagc tcaagcacaa atggcaaatt ttgtcgtatc aaatacttca aaaaataaag
     2701 aaaaatcagt agaacttcta gggctgctta acagtgatcc agaattattg aatgggcttg
     2761 tttgggggat cgagggagaa gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat
     2821 tgctagatgg ttatcagcca aatacacata tgtctgcttg gaatacagga aacaacaaga
     2881 ttctatatac acaagagtct attacggatg acatgattgc caaacgtgat caatcgattg
     2941 ctgatgcaga aacttcaccg attttaggat tcagcttcaa cactgacagt gtcaaaacag
     3001 aattaagtaa tatttctaat gtcatgaatc aatatttaga tggattgaat acagggactg
     3061 ttgatccaga tgaaacatta ccgaaattga aagacgcttt ggacaaggct ggctatgata
     3121 aggtattgaa agaaatgcaa aaacaatatg atgagttccg acaagaataa taatgaataa
     3181 agacaaaggg gttgtgacaa aagacgtgtc acaacctctt gttccgaata aacggtgttc
     3241 aaaagccagt ttctcggtat taagctaaat atatgtaaaa atgtggaaag ccatattcac
     3301 aaaatttttc ttactactcg aaactaagcg gctatttcac aaactcttgg taaacggtgt
     3361 tcaatcagct aaccctcggt attcgctcac aaaacgacaa aatatgagga actatttttc
     3421 cgtttgtttc tctaataatc aggtcaaggt gctgattgcc caaccttttt ttgtataatg
     3481 agaaagtata aacttaatgt ttggaggaga agatatgtta gggaaggttt tggaagagtt
     3541 atttatccgg atctgggtca ttataaaact aaccttgtat ttctggatat atacatttgc
     3601 cggcgggatc atctttggat taggtgcggc atggaaaaca gtcaatgaac tattttattt
     3661 gtacggattc gagtataaag aaatcacaat aaaaagaggc tggaatatat ataaaagaaa
     3721 ttttttaaga ggcaatctgc tatttagttt atttttatct ggtactgctt tattgagtta
     3781 caatttatat ttatctgttc aaatcaaagg gctcattttt ttagctattg attttatttt
     3841 actttttgcc ttgctctgcc tatttgctac ttatcaatac agtatgattt tagatagtga
     3901 atatacgatc tctgttccta atctgctaaa gctaagtttt atttcggttt tttccagctt
     3961 ttcgtctttt ctaaaaataa ttattggtag cggtataatt ttaggcgtga cttggcaatt
     4021 caaaggattg atcctgtttg gtgtcattgg tcttttgacg gtttggaatg gtacgatgac
     4081 aacacattgg cgtgaagaat tagataaaca gttggaaagt tatgaataag tttcggattc
     4141 tattttcaaa aggaacctta ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg
     4201 gtgtaacgat gactgctacc gttattttta gtatccatac ttgggaacaa aatcaaaaac
     4261 aggcagaaaa catgacaagt gatgccgttc aaagcacaag cagaatgctg aatgataaaa
     4321 caaccttgag taggatcatc aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg
     4381 tgacgaccta cttaacaaaa cccatagatc agtatctgat gtatgtatac gaacagcaaa
     4441 acagtaccga tgaattagtt tctttcccaa atcaaatcaa agatctctac gcaaattatg
     4501 aagaactaag tgcgatatat atcgtgttga atcaactacc ggagtattat gaatcttcta
     4561 gagaaaacaa aggtggggaa ataaaaaagg ggacaccaaa attactagac gccttttata
     4621 tcaagttgcc tatcacacaa ggaggagctg agtcaggaag tatttttatt ggttttcaaa
     4681 agaaagaact agatatgatt ttagaaaatc taacgtcatt caatggactc tctttataca
     4741 tgatttctgg aacgacaaat cgactataca cattccatga taataaaata gctaaagaaa
     4801 cgtttgaaag acaagaaatg atcatcacag agagtctgaa gaaaaattca caattgccga
     4861 ttgacgatct ggaaaaaaac aattttgttc aacaccaaga actgtctgat gattatcgta
     4921 tattagctgt actagataaa aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc
     4981 tgatcattgg tatcattatt ttagacaata tactgcttat ctttctttat aaaacattca
     5041 aacgatattc acagcaagta tctatcgtca tgcaggccat ggatcaaaca gcagcaggga
     5101 atttagagac gcggatcgac ataacaaaca cggaatatga actgaaggaa ctttcaatcg
     5161 gtatcaacga aatgttggat agcatcaatc agtttgtaga agacatctac aaattagaaa
     5221 tcaaacagca agacgcacat atgcgggcgc ttcaagcaca gatcaatcca cactttttat
     5281 ataatacatt agaatatatc cgaatgtatg caatcagcga aggaagtgaa gaattagcag
     5341 atgttgtgta tgcgttttcc gctttgcttc gaaacaatac gaatcaagag aaaacgatca
     5401 ctttaaaaga agaactggac ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc
     5461 ctaatcgagt agcctatcat tttatgatcg atcctgattt ggaaaagatc gaagtaccta
     5521 aatttgtgat ccagccatta gttgaaaatt attttaaaca cggtattgat tttactcgat
     5581 tcgataatgc gctaagtgtc aaagtcttgc aggaaggcaa acgggtaagg atcatcatca
     5641 aagataatgg aaaagggatg actgaaaaac ggctaaaaca gattgaagaa aaactgtctc
     5701 atcctaaagt ggaacttcac ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg
     5761 ccagcttcgg cagcagttat tatatgtcgc ttgagaataa tgagacaggt ggactgaccg
     5821 tttctattac attcaaggag ggatgagcgt gtatcaagtg ttattggtag atgatgaata
     5881 catgattttg aatgggttaa aaaagataat tgattggcaa agtttaggct ttcaaatagt
     5941 agcaacagca gagaatgcca aagaaggact agctgtactt gaacagcgac agattgattt
     6001 agtagtaacg gatgtgacaa tgccagaaat aaatggactg gaatttattg aagcggctca
     6061 aaaagaacgt cataattttg aatttatgat tttatcagga taccaagaat tcgattattt
     6121 gaaaggaggt atgcaattag gcgccgtgaa ttatttgatg aaacccgtga ataagttcga
     6181 attggttgaa agcctcaaga aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa
     6241 tcaacaagaa atctatcaag aactgttatt cagtcaatgg ttgaatgaag aattggatga
     6301 aataagtgaa gaagaaataa taggacaaat aagtgagaag cagcatagga tagtattgat
     6361 tcaactggca agaactcatg atactcttat caatagatgg ttgcaagaac ggcaacaaat
     6421 cttttactat caacgaaatt acggagaccg tgtattgatc acgttgttgt tggaggcaga
     6481 tgaagttgat ggcttctgtt gttttgttca agaaaacttt cctgatcagg aatggctgat
     6541 cagtatcggg gaagagacag attctttgga caaaatccct gaaacattcc aacaagcgaa
     6601 agacaatcta cagttgcatc aattttatgg agataaagac gagcatgttt tttacgcagt
     6661 acaagcagaa acaaaagatc aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa
     6721 taaacaattg gaagtcgctc aaaaaatgat cactgatttt tttgaacagt ttcagttagc
     6781 agccatgatg ccggaagata tacgatattc cagtttttta ctgtttatgg agattcaacg
     6841 ggaattgatt gatctggaag atgaggaata tttacaagga atcgaaaaaa tccatcaggc
     6901 aaaaacggtg caagaacttc agcagctact tctgtcattc atcaaaaagc atcagagaca
     6961 gaaaaagtac agtgacaatg tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga
     7021 accacttaca ctcaaagaag tgtcagaaaa cctgcattta aatgtcatgt atctaggaca
     7081 gctgttcaaa aaagaaacga aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga
     7141 aaaagcgaaa caactgcttt tgcacagtaa tcaaaacatc aatgagatag caagtgagat
     7201 tggctacaac aacaccacgt atttctctaa attattcaag aaaatcgtcg gcagatcccc
     7261 taaagagtac cgagaaaaca gtggagaatc ataaaagaaa aagccaatgt aatgaaataa
     7321 cttataaaaa attgtctaga aatctaattt tgggctgttc ttattacttg aggcaaggca
     7381 gctttttcat gacctctcga taaacggagt tcaaaagcca gtttctcggt attaagccaa
     7441 atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttaatact cgaaactaaa
     7501 cagctttttc acgacctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct
     7561 caaaaaatgg caaaatatga ggaactattt tgccatttgt ctctctaata ctcaggtcag
     7621 aacgctgatt tcacaacctc tcaataaacg gtgttcaaaa gcttgcatct gcggtaataa
     7681 gaccaaccaa acgaaaaata tgaggatcta tttttgcttg gctgttctta ttacttgatg
     7741 caggacagct ttttcacaac ctcttgttaa ttatagtttg ataaggaaaa aggcaaatct
     7801 tttgcttgat atttcttagg gtgagggatg atccctaatt ggtaagagat cgtgccgcct
     7861 ttcagcaaat cttgatgggt aaaatataat ttcgtatatt ctttgtccat ccgtttgatt
     7921 ttatggataa attgttgctg cggccgatta ggcgttgctt caatcgtcaa ttcctttcca
     7981 tttgacaaaa gcaaaacagc cttgtcaatt aatggcatac cgataacata ttcaccggtt
     8041 ccaggcgtga caggataaaa tcccagacta ttgaagatat accaagcggc catacttcca
     8101 ttgtcctcat ctccagggta acccattgga gaatcgtcga aggtctgagt catcaactgt
     8161 ttaagcagtg gctgtgccat ttcaggttgt cttatataac tgaacaagta aggatagtga
     8221 aaacttggct ggtttgaaat agctaattgt ccaaattcta tcgcggccat ctcactcatt
     8281 tcgtggatct caaaaccata tcctcctggg tcaaacgttg ggggctgatt gcacagctca
     8341 atgagttttt ctttatatct ctcactgcca ccatattttc cgatcagtcc tgaaaaatcg
     8401 tgataaacag caaagctgct ttgccaagca cttccttctg cataatcttg tccccaacga
     8461 ttagctgaaa atggttctct gaacttgcca tctttatctt ttgaacgcat aaatcctgac
     8521 tcatagtcaa agatattttg atagttcaat gattgctgct gatatttctt cgtcatttcc
     8581 tcttcttcta gtaccttggc aacttggcta atacaaaaat cgctataagc atagtctaat
     8641 gtgtgattga ctgattcgtg gtagtgatta ggaacatagc catatgtcaa gtaatcttct
     8701 gttccttggc gtccataatt tggatgtttg ctttgaaccg tagcaccttt ttccattgcc
     8761 gttagaaatt caggcatcaa gtctcgtcgg atatctttta cagcagcatc tgcaatgacc
     8821 gcatcgatca atgtcccagg cataagtcca cgttcatcag gagacagcca ttttggcaaa
     8881 tagccacttt ctctatagct gttcaaaaaa ccttccagca tttcttcgta tttttctaca
     8941 gcaatcaaag aataaagagg ataaaccgtt ttatacgtat cccaaaaccc attatttgta
     9001 tagagaacac caggttttat agatcgacta gtcgtatcat agtggatttt ttgcccacta
     9061 ggatcaattt cataaaatgt ttgtggaaat aaaaacaccc gatataaatt atgataaaac
     9121 gtccgcaatt gttgttgatc atggtgtgtg agctgtatac gatcaaaata ggactgccat
     9181 tgttcttgac tgtgagcaag gtaagtctct ttgctttggt tttgttctag ttccaggtta
     9241 taagctgctt gttccgagct gataaaagat gtcccaaatc ggatagtttg tttttttgtc
     9301 tgttcaaaat acaacgggat cgagtcgcta tttttgttgg aaacagctaa ctcatcaatt
     9361 gttaaaggat attcaaatct caatataaaa taaaaagaaa aatcaggatc ttctgaacca
     9421 gcaaaatgtg tgatttttcc ttccactgtg taaggatcta taacaaagag ctgatggcgt
     9481 cccggaaagg ataaaagtaa accgctgtca tttttgctgt aatcaataga taagattccg
     9541 ccatacatgc taggtatcaa tgtagaacag atgccataac gtaattgaca gatcgacaga
     9601 tgagtcgggc aaaatatact ttcttctggg cggtaagaac tttgagcatg gaaaagggtg
     9661 ttctcctgta gttttccatt gatcggcgta aacagcatat gactgaaatc acccatccaa
     9721 ggacttggtt gatgggtaag acggtagcct tgaaaaacgc ggtcatcagg atgaaaccac
     9781 cagctgccgt tttgatccgt agtttgcggt gcaaaataat tcatcccaaa aggaacgcca
     9841 gtataaggct ggcagttccc attagagaaa tttggctgat tcgctgttgc atgtctagta
     9901 tctatttgac cgatattcat tttatttctc ctttgttgat tgtcttttga tgaatgaaac
     9961 tggaaattta ctatcggatg gtcgatcttt tgtctctaac caagtgacca aagcatctcc
    10021 cgcaagctta cctaactgtt caaaatcttg tgcgatggtt gtcagtggcg ggtcaacaag
    10081 agctgctgct tggatatcat caaatccgat aattgaaaaa tcttcaggga tatgataacc
    10141 atgctgtctc aagagattca tggtctggat agcgacaaca tcgttttcac aaacaaaagc
    10201 agttacttca tacttagtga tataagaaag caattcatct aatgtcgcgc ttttgtcatc
    10261 cagtgaagtg tggaaagtca aaccagcttt gtccaatgct tcaaggtaac caaggtaacg
    10321 ctgtcgaacg gattgtggat gagtctgcgt accaaacaaa taagcgattt ttgaatgtcc
    10381 ctgttcgatc aaataagagg cagcaagcgc accgccttgg aaattatcgg aaagaacggt
    10441 aggaaaaggt aactcataga tttttttatc taatatgacg acagggaatt ctttcaatga
    10501 aagctcaaaa agtaagtcaa gatgttgttc tgtatcaaga gcataataaa tcaatccgtc
    10561 aaattcattg attagctctt ttgctgtttt attttgtaga aagtcaagcg tcgtcatcat
    10621 gacatcaatc tgtttatctt gcatcacagg gttcaatccc ttgctaaaat caccaacaga
    10681 caaatcagcg acatatggaa gaagaaacag aatccgtcgt gtttttgtag gagaagcaag
    10741 aatctgttcc ggagaagcgg atttaacgaa actgcctttc ccgcgaacgc gataaataag
    10801 tcctaattgc tctaattcag tcaatgcacg tttggaagtg attctgctta ctttataggt
    10861 acgtgaaagt tctttttcag ttggtagttg gctgtctaca ggtaattcac cagttaaaat
    10921 agccgtttgc aaatcattaa atattgtctg gtacaatggt tgattcatat aaacacctct
    10981 taatgatata tcatattcaa ataataccat aatggatgaa aaagtaaata aaaagatatt
    11041 t
//
LOCUS       DAXWLF010000092        10574 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXWLF010000092 DAXWLF010000000
VERSION     DAXWLF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.092"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            12..84
                     /locus_tag="ACGE4U_001759"
     tRNA            12..84
                     /locus_tag="ACGE4U_001759"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACGE4U_001760"
     tRNA            88..160
                     /locus_tag="ACGE4U_001760"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACGE4U_001761"
     tRNA            168..249
                     /locus_tag="ACGE4U_001761"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACGE4U_001762"
     tRNA            267..339
                     /locus_tag="ACGE4U_001762"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACGE4U_001763"
     tRNA            351..422
                     /locus_tag="ACGE4U_001763"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACGE4U_001764"
     tRNA            439..524
                     /locus_tag="ACGE4U_001764"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACGE4U_001765"
     tRNA            535..608
                     /locus_tag="ACGE4U_001765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACGE4U_001766"
     tRNA            618..691
                     /locus_tag="ACGE4U_001766"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACGE4U_001767"
     tRNA            711..783
                     /locus_tag="ACGE4U_001767"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACGE4U_001768"
     tRNA            811..884
                     /locus_tag="ACGE4U_001768"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACGE4U_001769"
     tRNA            898..971
                     /locus_tag="ACGE4U_001769"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACGE4U_001770"
     tRNA            995..1084
                     /locus_tag="ACGE4U_001770"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACGE4U_001771"
     tRNA            1097..1170
                     /locus_tag="ACGE4U_001771"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACGE4U_001772"
     tRNA            1189..1264
                     /locus_tag="ACGE4U_001772"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACGE4U_001773"
     tRNA            1266..1336
                     /locus_tag="ACGE4U_001773"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACGE4U_001774"
     tRNA            1350..1423
                     /locus_tag="ACGE4U_001774"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACGE4U_001775"
     tRNA            1448..1535
                     /locus_tag="ACGE4U_001775"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACGE4U_001776"
     tRNA            1557..1628
                     /locus_tag="ACGE4U_001776"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACGE4U_001777"
     tRNA            1651..1740
                     /locus_tag="ACGE4U_001777"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACGE4U_001778"
     tRNA            1753..1826
                     /locus_tag="ACGE4U_001778"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACGE4U_001779"
     tRNA            1845..1917
                     /locus_tag="ACGE4U_001779"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACGE4U_001780"
     tRNA            1921..1993
                     /locus_tag="ACGE4U_001780"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACGE4U_001781"
     tRNA            1998..2068
                     /locus_tag="ACGE4U_001781"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACGE4U_001782"
     tRNA            2081..2154
                     /locus_tag="ACGE4U_001782"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACGE4U_001783"
     tRNA            2166..2237
                     /locus_tag="ACGE4U_001783"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACGE4U_001784"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACGE4U_001784"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFF8393921.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACGE4U_001785"
     CDS             3826..5298
                     /locus_tag="ACGE4U_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFF8393922.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACGE4U_001786"
     CDS             5381..6169
                     /locus_tag="ACGE4U_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8393923.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACGE4U_001787"
     CDS             complement(6236..6904)
                     /locus_tag="ACGE4U_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFF8393924.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACGE4U_001788"
     CDS             7019..8731
                     /locus_tag="ACGE4U_001788"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393925.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACGE4U_001789"
     CDS             8734..10506
                     /locus_tag="ACGE4U_001789"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393926.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3033 a         1926 c         2453 g         3162 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggca
//
LOCUS       DAXWLF010000093        10550 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXWLF010000093 DAXWLF010000000
VERSION     DAXWLF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.093"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            34..657
                     /locus_tag="ACGE4U_001790"
     CDS             34..657
                     /locus_tag="ACGE4U_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393927.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACGE4U_001791"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACGE4U_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8393928.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACGE4U_001792"
     CDS             2153..3034
                     /locus_tag="ACGE4U_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8393929.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACGE4U_001793"
     CDS             3037..3807
                     /locus_tag="ACGE4U_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8393930.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACGE4U_001794"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACGE4U_001794"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFF8393931.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACGE4U_001795"
     CDS             5118..6281
                     /locus_tag="ACGE4U_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8393932.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACGE4U_001796"
     CDS             6398..6808
                     /locus_tag="ACGE4U_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8393933.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACGE4U_001797"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACGE4U_001797"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8393934.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSNLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACGE4U_001798"
     CDS             9381..9665
                     /locus_tag="ACGE4U_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393935.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="ACGE4U_001799"
     CDS             9825..10061
                     /locus_tag="ACGE4U_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8393936.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="ACGE4U_001800"
     CDS             10058..10456
                     /locus_tag="ACGE4U_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFF8393937.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3678 a         1770 c         2229 g         2873 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctaat
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat
//
LOCUS       DAXWLF010000094        10353 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXWLF010000094 DAXWLF010000000
VERSION     DAXWLF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.094"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            219..494
                     /locus_tag="ACGE4U_001801"
     CDS             219..494
                     /locus_tag="ACGE4U_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393938.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            507..2585
                     /locus_tag="ACGE4U_001802"
     CDS             507..2585
                     /locus_tag="ACGE4U_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8393939.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2748..4631
                     /locus_tag="ACGE4U_001803"
     CDS             2748..4631
                     /locus_tag="ACGE4U_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8393940.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4643..5590
                     /locus_tag="ACGE4U_001804"
     CDS             4643..5590
                     /locus_tag="ACGE4U_001804"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFF8393941.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5610..6137
                     /locus_tag="ACGE4U_001805"
     CDS             5610..6137
                     /locus_tag="ACGE4U_001805"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFF8393942.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6200..6853)
                     /locus_tag="ACGE4U_001806"
     CDS             complement(6200..6853)
                     /locus_tag="ACGE4U_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFF8393943.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            6985..7827
                     /locus_tag="ACGE4U_001807"
     CDS             6985..7827
                     /locus_tag="ACGE4U_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8393944.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            7985..8881
                     /locus_tag="ACGE4U_001808"
     CDS             7985..8881
                     /locus_tag="ACGE4U_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8393945.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8884..9597
                     /locus_tag="ACGE4U_001809"
     CDS             8884..9597
                     /locus_tag="ACGE4U_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFF8393946.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9617..10135
                     /gene="msrA"
                     /locus_tag="ACGE4U_001810"
     CDS             9617..10135
                     /gene="msrA"
                     /locus_tag="ACGE4U_001810"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8393947.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10132..>10353
                     /locus_tag="ACGE4U_001811"
     CDS             10132..>10353
                     /locus_tag="ACGE4U_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFF8393948.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHD"
BASE COUNT         3597 a         1767 c         2145 g         2844 t
ORIGIN      
        1 acaagaaagg caggaatggg aaagtaaaca tccttttgtc agaaactttt atctttctta
       61 agttttactt gatatctgat actatttgga atagaatgag gcgttgaaaa atttatggaa
      121 atttctaact agcaataaac gatgaaacaa tgaaatattt ttcatccaaa aagaactttg
      181 ctataatgat ggaagtttgt gtataggaga tctggaaaat gagagagagt tttgaacaac
      241 aaaaaaaact gttgcatgat cgctatggcg ccctttcgat ggatgaccgc cgtcaaatac
      301 tttgtaagtt gcgtaaaaga aatatattga tgtatcgtca gttggaacgt ctgaaacatg
      361 atctgcttcg tttggagtca aaacgtgtac agtgtgaatt agaggggaac caaacacagg
      421 ttgaagtagt cgaaaccaaa attttaaaga aaaaagaaca atttttaaaa atgcttacac
      481 aaaataaaaa ataaagggga tggacgatgg aatttgctga gttagttatt gtatttgcag
      541 taacgatcac cttatcgaac atagctagcc gcattttgcc aatgattcca gctccactga
      601 tccaaatttt ccttggagtg gtattaggtc tgacagagtg gggacaatca atcgattttg
      661 aacctgaatt gttcttagtt atgattattg caccattgtt atttagagaa ggagaaaaag
      721 cagatatttc ttcgattctc aaaaatttcg ggacaatttt gttcttggca tttggaggag
      781 tcattttgac ccttgtcggg gtaggtgcga cgttatcatt cctgttgcca agtgtcccac
      841 ttgctgcctg ctttgctttt ggagcagcgt taggtccgac agatgcggta gcagtcagtt
      901 ctctttctgg acgagtgaat atccctaaaa aggcgatgca tattttggaa ggagaaggtc
      961 ttttgaatga tgcatctggt gttactgcct tccagtttgc tcttggtgcg ctgatcaccg
     1021 gatctttttc agcagtaaac gcggggatga gtctagtagt ttctagtatt gggggagctt
     1081 tgatcggctt tttgcttgtc tggttcaaac aaaaaataat tcatttgatc gaaaaagcat
     1141 cagcacaaga tgtcacaggg tatctgctga tcgaactttt gcttcctttt ctagcctatg
     1201 tattggcaga ggcatttgat gtatctggaa tcattgctgc agtggcagca ggtattctcc
     1261 aagcttctgg ttttaggaaa atctctgtgt ttgatgcaga attgtcgagc ttgtcccaca
     1321 gtacatggac aactattgct tttacactga atgcattagt atttatattt ttaggaatcg
     1381 aattgacgca agtgttctcg ccagtatggg gagacggttt atatccaaac ggtcttttat
     1441 tagcgattat cgtattaatc tctgtcatgc tttttgtgat cagattcatt tctatcagtc
     1501 ttttttatgt attcaaagat ggcagtaaaa agttcaaaaa acagttgaac gagatcttga
     1561 tcttaacatt tggcggtgta aagggaacag tcagtttggc tacgattttt attttgccat
     1621 tttcaatcaa taatatgatg ttttaccagc ggtctttgct tttgtttctg acggcgggtg
     1681 tcatcttagt gactttggtc atagggatca ttgtcttgcc tatgctgacg gaaacagagg
     1741 aagcgaaaag cacggatttg aatgctttga tgattttaga agaggtcgtt gaagttttgc
     1801 gaaaagaaat aaaggaaatt gatcgtaata caaaagaatt tttagccact gaagcagtga
     1861 tagaaaatta tcaagaacgg atacgtgatc tgtacttgga agatttaacc gatgatgaaa
     1921 agcaagaagt acaagaaata caagcactga ttttgtcgat cgaacgggat ggattggatg
     1981 aaagttatcg ttcgggcaag ctttcttcta atggttatcg tttttactcc cgttttcttt
     2041 ctcgattcga acattctatc acaagccaga ttttgtcttt tatcggcttt tggttcattg
     2101 ttgtccgtag gttaatgcga atcgttctcc atccgaaaat gttttggcaa agaagatatg
     2161 ctgatcgtca gacatttatt agtgatgaag atatccaaga aatcagagag acatatcaaa
     2221 aaaatactca gttgattatt gaaagtttag ataacttgac tgatatttat gatgaaacga
     2281 tggttcaatt ttttatccag caacgcaaac ttgaaggaat aaaaatgaac tcggggaatt
     2341 tgatttcttc gtggatgatc cagcaagata gtctgtttac gaaaaaaatg ctaagaggtt
     2401 attatcttga aagaaaaatc attgatgaat acgaagttgc tgaaaaaatc acgacgtttt
     2461 ctgcgaacag ctatcgcagg aatatcaatt tgttagaatc ttatacgatg aacaaaccat
     2521 ctgataactt ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg agtccaataa
     2581 aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga acaggatcga
     2641 atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa attttcctga
     2701 agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg aaagaattaa
     2761 aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag atttcttttt
     2821 tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt aaaacaagtc
     2881 tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca gtgttttatc
     2941 caactgatta ccgtattggt tatctttctc aagcagctga attttccgat gaattaactg
     3001 ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga gcatatgagg
     3061 aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa cgttatgcta
     3121 aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac gcaaagatta
     3181 ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta tcaggtggtc
     3241 agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta ctattactag
     3301 acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac tatttgaaac
     3361 aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat agagtagcaa
     3421 accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat tatgaagctt
     3481 acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa aagcgcaagc
     3541 ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt ggcaccaagc
     3601 agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa gtaaaccaag
     3661 aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta ctggaaatca
     3721 aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat ttattgattc
     3781 aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg acattgctta
     3841 atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt gaaacggttc
     3901 atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg atgattgctt
     3961 acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc ggagtagcag
     4021 aattacttga acgattttta tttccaagat ttatgcatgg gacactgata ggcaaacttt
     4081 caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa cctaatgttt
     4141 tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt ttggaagatt
     4201 attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat ttcttggaca
     4261 aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt tattacggaa
     4321 acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa aaagcgaaac
     4381 caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg gaacaaaaag
     4441 aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca ttgctccaag
     4501 aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa aacgacgtat
     4561 ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg agtgaatggg
     4621 tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa gaaaattgtt
     4681 ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct tattcggata
     4741 ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac gagtaccttt
     4801 tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata tccgctatct
     4861 attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca tcaaaagtcc
     4921 agactataat ggaccagata tgactgattt tggccataga tgtttgacag atcctgaatt
     4981 caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa ccgatgattc
     5041 ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc gtcactggga
     5101 aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa tcaaaaagac
     5161 tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc ctagcatggc
     5221 tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc taagctgcca
     5281 attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg caagttatgc
     5341 gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat ttgttcacac
     5401 attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac aattgagccg
     5461 agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag tatttgattt
     5521 tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca aagctcctat
     5581 cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca caggatgaaa
     5641 acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat ctgaaattct
     5701 tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc gaagggatgg
     5761 gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct tcctataaag
     5821 cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa aaagaaacag
     5881 acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg ccattttgta
     5941 cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat tttccacctg
     6001 ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat gaaaaaaatc
     6061 gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa agcgggacag
     6121 gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag ctgtcccatc
     6181 catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata aaacctgcac
     6241 ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc aagctataaa
     6301 agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca ccagtcattc
     6361 caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata aaaatataga
     6421 tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct ttatatataa
     6481 tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc catcctcgga
     6541 tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc gaactatgat
     6601 cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac aaagtagacg
     6661 ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata tggagggagg
     6721 aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc aatccagtcc
     6781 caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt cgattaaatt
     6841 tcgttttttc catgaaaacg gctcctttac taggtgtaat taatgaaaaa tcattatttg
     6901 tctgttatac taaaaataga aattgtctta catgtagttt aacatgaata aggcatcttg
     6961 ttaaagaaag agagagagaa aataatgaca aatgtaaaaa tcgtaacaga ttcttcatgt
     7021 acgatggaaa aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct atccatcatg
     7081 atcgacggaa cagtatatcc cgatgatgac caactagacg gcgaaagatt tatggaaatg
     7141 atggcgaaag caaaaaatct gcccaaaaca agccaaccac caattggaga attcatctcg
     7201 atgtatgatg agctaggaga agacggcagt gaagtgattt cgatccatat gacaaaaggc
     7261 ttgagtggaa cagtagaagc agcaagacaa gccagtaatc taacaaaaac gaaagtaacg
     7321 gttatcgaca gtgataccac tgaccaagga ttatcttttc aggtaattcg agcagctgaa
     7381 ttagcaaaag aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa agtacaagac
     7441 aatacaaaat tatacatcgg aatatccaca ctagataact tggtaaaagg tggaagaatc
     7501 agccgaacaa ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat gaatttcgcc
     7561 cattctgaat taatccctgt aactaagggg cgaggaaaga aaacgttcga taaatggttt
     7621 aataatttga aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg tatttcacat
     7681 gcggatgcaa aagaattggc gcaagaattc aaagagggtt tgcaagaatt attcccggac
     7741 atgcatatcc ctgtactgca tacgaatccg gttatcgcta cgcatactgg taaagacgcg
     7801 tttgcaatca tgtattatgt agattagtcg aaaagaggat aagccctcag cctgaaaggc
     7861 ttgcagctta ttctcttttt ctttataaaa ttaggaaaat aattgttttc ttttttcata
     7921 aagactacct ttttgataaa atttttgata acattagaac caatcgaata caagaggtga
     7981 aaaaatgaaa ttaacgaaac gacatttgat cctcgttttg ttcccattat taattgcgat
     8041 catcatctat gcaggtttag ctgcttctat accgaaagct ggcccactaa tgaaaccagt
     8101 aactactgtg agagaagatc caaataaaaa agaaaaactt cactacatag ctatcggaga
     8161 ttcgctgact gaaggagtag gagatcaaac caagcaagga ggattcgttc cccttgtcgc
     8221 aaatgatcta aaagaccgtt ataatttgac cgcggttgaa acagaaaact atggtgtaaa
     8281 tggtgaacga agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa ttcgaaacaa
     8341 tatcaaaaca gctgatttta tcacactgac tgttggcggt aatgatttga tgaaagttat
     8401 ccaaaataat ttattcgggt taacggtcaa ttcctttaaa aagccactga aaaactatca
     8461 ggaaaatgtc acagaactat tggaagaatt tcgatccctc aataaaaccg cacctattta
     8521 cattttaggc atctacaatc cattttattt gaactttcct gagatcactg acatgcagac
     8581 aattgttaac aattggaaca aaggaacaga aacggtgaca aaaaaaatgc cgaactgcta
     8641 ctttatccca atcaatgatt tgctttatca aggattgaat caggaagtag gtgtcacaga
     8701 agaaggaaca gatcaaacaa ctagcacgac agaaaatcta aatggcatca aaaataatgt
     8761 gctctatgaa gaagatcatt tccatccaaa taatttaggt tatcaattga tggcaaatgc
     8821 agtgagagat cagctgatcg agacacaaaa ccaatggtta gagaaggagg atgatcaata
     8881 aacatgaaaa caagatctga cgtatcttca aacaaaacaa agaaaacaaa ttactggaaa
     8941 ttcgcattcc ttatccttct tggggtagta cttggttcga ccgtattttt aggcagccgt
     9001 atttttgcta atcgggaacc agaactccca gaaataccag ctcttacgga aagacaagga
     9061 gatcctgtct taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat cagctttttc
     9121 cttagtgagt atcaaaaaga tagtgatatt gaatataaat tttatttgga aaatgaagca
     9181 ctactcaatg gtactttcga agtactaggt tttcctatcg atttctattt atattttgat
     9241 ccgtatgtga tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc tatcggaaca
     9301 ttgagtttgc caatcaaaga tgtgatgaat atgatcaaga gaaactataa attaccagat
     9361 tggatcgaag tcaatacaga tgatctgact atcatgctta ggttggatca attccgaatg
     9421 caaaacggga tgtatattaa agcagataaa atcgacttag taaatgatga cattcggttt
     9481 agtctatatt tgccaaaaga tacctctgat gcttcaaaca actcaaatac caacaaaact
     9541 agcaccagca gtactgaaag caagacaaaa gaaaataaaa aatcatcgga tgagtagtat
     9601 tagaggggga atcataatgg aacgagcaat atttgcagga ggctgcttct ggtgtatgat
     9661 ccagcctttc gacacgcttc ctggtatcca tacagtgatg tcaggatata ctggagggca
     9721 tgtaccagat ccgacttatg aacaagtaaa aagccaaacg actggacata cagaagcagt
     9781 ggagattatc tatgatccaa cattgatttc atatgaacaa ttagtagaac tttactggca
     9841 gcaaactgat ccaactgacg ctttcggaca attcgaggat cgcggtgaca attatcgccc
     9901 ggtgatcttt tatttgacag aagaacagcg gatcattgct gaaaaaagta agaaacagtt
     9961 agctgaaagt ggacgtttcg aacagccaat cgttacaaaa atcgaaccag cccaaccatt
    10021 ttatctagca gaagaagacc atcaagagta ttataaaaag catcctgaaa attttaaaag
    10081 aaaccatgca cgccgggctg cctttatcga agaaaattgg aggaacgaag ggtgaatcga
    10141 agcttttttc attatttaat gacactgcga ggcggtggca cacatgatgc tttatctgtt
    10201 ttcgctacag aagccggtaa agacatccaa tttcctaagc acagctctag ctacgaagaa
    10261 atttctaatt atctggaact gaatgtcgac tatttgccgt ccatggatat ctttgacgaa
    10321 gcatgggaaa aatatttaga aaataatcat gac
//
LOCUS       DAXWLF010000095        10212 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXWLF010000095 DAXWLF010000000
VERSION     DAXWLF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.095"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..1283)
                     /locus_tag="ACGE4U_001812"
     CDS             complement(<1..1283)
                     /locus_tag="ACGE4U_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFF8393949.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
     gene            1527..2174
                     /locus_tag="ACGE4U_001813"
     CDS             1527..2174
                     /locus_tag="ACGE4U_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393950.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(2267..4546)
                     /gene="helD"
                     /locus_tag="ACGE4U_001814"
     CDS             complement(2267..4546)
                     /gene="helD"
                     /locus_tag="ACGE4U_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFF8393951.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKNLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            4824..6647
                     /locus_tag="ACGE4U_001815"
     CDS             4824..6647
                     /locus_tag="ACGE4U_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8393952.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            6694..6873
                     /locus_tag="ACGE4U_001816"
     CDS             6694..6873
                     /locus_tag="ACGE4U_001816"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFF8393953.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            7210..9150
                     /gene="thrS"
                     /locus_tag="ACGE4U_001817"
     CDS             7210..9150
                     /gene="thrS"
                     /locus_tag="ACGE4U_001817"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFF8393954.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            9374..>10212
                     /locus_tag="ACGE4U_001818"
     CDS             9374..>10212
                     /locus_tag="ACGE4U_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8393955.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFM"
BASE COUNT         3173 a         1837 c         2142 g         3060 t
ORIGIN      
        1 attaattgat tgatcaaaaa gattctaatt ctcatgttct caaatgattt aaatccgtag
       61 gatactcgtt tcattgtctt tatatgggta ttcttcgctt ctatttttcc attggtgtaa
      121 ggatagatca tcgcgttggt gatgccttct tcataggtca gaaggttttg gagcttttcc
      181 cgaaagccgt catctaacgt ttcgggaagt tctgccaata aggagaaaaa taagtcaggg
      241 tctttgtctc gaaaggcttc aactaattca tgaaaatagg gatacgcctc cttcagggac
      301 gtagaaaagc caagcaatcg atcaatcatc atcgcttccg taagaaatgg gtattttggt
      361 gctcgaaaac ttttccatgt tttgtattca taatggttga tgtttgcacg attttttagc
      421 aaaaagcgcc agttcttttt cagtttttct gcctgacttt tctgtcctac tttacgaagt
      481 tcattcattt cacggatacg caactcatta aaggcttgat tcatgtgttt gataatatga
      541 aaccgatcaa tcactacttt cgcatttggc agaatacgtt tggtaagctg gaagtaggcg
      601 gcgttcatgt ctgtgactaa aaattttact tcttcaggat tagcgcagcc taagaaatag
      661 cttgttaatc gaggcaattt acgcgtaggc aagacatcaa ttaattgtcc tgtttcgcca
      721 tctgcgcaaa taaagctcat tttatcttct atggaagcgt gcgaacgaaa ttcatcaacc
      781 atcaatactc tggggagaat cctcttagat tgctttggta agtagctttt aaactctttc
      841 aacgtacgaa taacggtggt caaagatacc tgacaatttt tcgcaataaa agataaagat
      901 actttttcag tcagtaaaga agcaatttta tatctgacat gattcgcgat tgaatgtctg
      961 ggttgaacaa aataactttg agccgtccaa tgggttcgac agtttttaca ggtatagcgc
     1021 tgctttttta agcgcataac caaaggcatg tgattgtatt gttcaaagcg gacaatcgtt
     1081 tccttcttcc catttttcac tacaattact ttcccgtttc catctaccac agtagaacca
     1141 caatttctac aggtatgggg agcaggtgag aaaacagcat cgacgatcaa cgtctttttc
     1201 ttctgaaagg tctcgtaaga gacctctgta atcatcaaat ctttctctat taatcgcagc
     1261 atttttttga tagaatcatt catatagcat atcgtcctct cagttgttta ttttgtcgtg
     1321 atttaatcat actagagaac gatatgtttt ttaataccta aaataaaaat ggggctgaag
     1381 aatcaattct gattcatcag tcccataaat tatagagccc ttttttgtgt gatttatttt
     1441 cttttctaat tcaacctgtg gtatgatgag agcataagta tcatgtgaaa tgatatgaag
     1501 tatgtggaag atggacgggg aagaatatgg aagcaaaaat cactttagaa ccttttgaac
     1561 gtatcttaag cggttaccgc aaagtagaag agttagctgt gaacgtaact gattgttcaa
     1621 aattagcgca aaaatacgca agattcggcg ttgaaggcta tcgattagga aactatgtcg
     1681 gtacagggta tctgaaccgt tatttagagt gtatggtgga ccgcgcacca atgctgattt
     1741 atagacaaaa ttatctgatt ccgctgcttt ttcgtcgttc tgattcagcc tttcggttat
     1801 ttgaagaaga gtatcggatg gaggctttct ttctactatt agagtggagc ttgaaacatc
     1861 gtcctgaaaa aattcttatc gaaagaaatg agaagataga cacgaaaaaa aataaagtga
     1921 tcgatagtgc atacctagca tttcgtgtat cagagatttt ggattgtggc ggctatccaa
     1981 tcagcaattt ccaatcaatt gaccaattca ttgaatggaa tcgaatttac cgattgatcg
     2041 acaatggcgg gattggccgt catagcaagg tatttgaccc agaatatcca gaaaacatgg
     2101 aagaactgaa aatgattatt tcgcttgtca aattaaaata tcctgaaaca gatttggatt
     2161 tatatataga ataaatgacc ttaaatgatt gaattacttt ttctgcaatt attattgtaa
     2221 ataaaaagac aactgctaac attaatgata gcagttgtct ttttacttac agtacttttt
     2281 tctcataaga tgaaatatcc atattgttta acaaaggact ttcattattg gaggtaaaaa
     2341 gggtcaattc atgcatcgca cgtgtacaca tggtgtaaag gatcaatttg tcttgctgag
     2401 aagaaaaatg ttctgcatct acatcccaca agaaaactcg gtcaaactcc agaccttttg
     2461 ctaaaaaggc tggaagtaaa ataatggaac gcttcataaa gtcactctca tttaaaatca
     2521 gctggacttg ttgtttcaac tcgtctggca gttgagcata aagagcttcg cattcttctt
     2581 gtgttttagc tatgattgct gtacgccaat accgggcttt tccctgagtc gttcgaatgg
     2641 tatctgctaa ccaattcaat ttgatatcat ctgttgttcc ttggatgagc aaaggaaggt
     2701 ctccttttct tgctgttgtt tctacttgat gttctttcac aataaaatga ttagcaaaat
     2761 cagtgatttc ttttgtggag cgatagcttg tcgttaattg gaaacgagtg acttcacggt
     2821 ccacgaaaag gtcttccaat gatccaacga tcgtttcatt tccaaagaca ttttgattta
     2881 gatctccaca gaaagtcaaa tttgcttttg ggaacaaggc ttgcagcaat gccacttgtg
     2941 atggtgcgaa atcctgcatt tcatcaataa agatatagcg agctttgtgt gtaaccgtca
     3001 ctggatatgt tcggcgcatc agtaaaaaga atagtacagc atcctcttga cgcaattctc
     3061 tttgacgcaa gtgtgtacga acagttgtca aatgttcctg ccacctggtc tcgctgattt
     3121 tatgtcgtgt tagaagagaa agtggcacag tttgtagaaa gtggagatat tgtttcgtta
     3181 tattgacaaa ttgataattc atgatccctc gatggattcg acggaatctt ttacgtacga
     3241 tttggttggc caggttttta cgtaatgaac gctccttttg ttctgtatat tcaagatttg
     3301 gatctgttgc gtatagttct tggagctgtt cttccgccag atctttgacc cattgctgac
     3361 gtgtttcgtc tttttgcaga ccgcctaatt ttttcaggag cttcgtttgt aatagagaaa
     3421 gtctatgatg taaaggtaat tgttcatttg tttctttata ccattggcga atcgattcct
     3481 ttgataggat cgtacggcca tttattttca tatcacggaa caagggaccg tagtttgtga
     3541 tgccttggat atagcgatca atctgatcga ctaaggtcaa tccgcttttc atcacttgta
     3601 tcggatcttt ttcccctagc aaaaaaccac tttcttgttg ctgttcatct agtaaagaaa
     3661 actctggcag cagctgcacc aaataatttt tgaatgtctg tgtaggcacg ccgctttcac
     3721 ctaatgaagg caatacagtc gagatgtaat cagaaaacaa atggttgggt gaaaatagaa
     3781 gaacgttttc agcctcgagc cacttgcgat tatgatacaa cagaaaagca atacgttgaa
     3841 gcaaagcgga tgtctttcca cttccggcga ttccttcaat cagcattact ttacttgttg
     3901 tgtcccgaat gattgcattt tgtgcttttt gtatcgtaga gacgatgttt ttcatatggt
     3961 tgcttgatga ttcatccaat atctctagca aaaagtcatc attgattact tccgacgtat
     4021 ctaccataga taaaatacgt ccttcttgga ttttaaattg tcgtttcaac tgcagatcga
     4081 catctattct atctgtttca gtttcataaa atgcgggacc aatctcacct tcataataaa
     4141 gattggcaat tggtgcgcgc cagtcaataa cgattgtttc ttctgccaga tctcttaaag
     4201 aagcgatacc tagatataat gtttctgttt cacggtcttc tttaaagtcg atacgggcaa
     4261 aataaggact atctttcatg acagacaacg tttgcaggcg tttttgctgt gcttcaagag
     4321 tatggtattt caaaagcaat tcctgctcat gttggcgata ttcaacagca gattcataaa
     4381 aagcttcatt ggaaccagct cgaatcttgt gattagctgt gtcttttgtc gaatgattca
     4441 tttcctgagt caattcttgt tgttttgtat ccagcatatt ttgttcgatc tggattagtt
     4501 cgatcgtttc atccacgtgc gtttgttcta gttgtttctc attcatcgca tgtttgctcc
     4561 ttccgccgta aataacgaat taaaagtata gcatagtttt tcgaaaaaag gtacttttta
     4621 actgatgaat cgaaaaagta atccttgctg aatatttttg tttgtagaac tgatgaattt
     4681 tggaaacgct gatagtgata gttgttttca attaaggcct attgttttaa aaagtgctag
     4741 tcaaggacta tgcttaagat gaaagaggca gctggaaatt tattactaac ctctctaagt
     4801 cacataaaag aggagaaaaa acaatgacac atttcaaaaa atttttactt actttgctta
     4861 ttggtgggat agctttatta agtgaatttg tttttcacca gccggccctt gcatttgcga
     4921 ttgtcgcatt tactggaggg attctcgcct tcttgatgtt tttggaaatg atcaagacgt
     4981 tacgttcagg aagatatggc gtagatgtat tagcgattac cgcaatcctt gcaacattag
     5041 ccgtcaaaga atactgggct agtttgatga tattgatcat gcttacgggc ggagatagtc
     5101 tagaggatta cgcgaataaa cgagcgggta aagaactgca gtctttgtta gataacaccc
     5161 caagaattgc ccatcgattc aaagaaggaa aattgcagga tatagtaatc gatgaaataa
     5221 atattgatga ccagttacta gtcaagcctg gcgaacttgt acccgcagat ggtcgagtga
     5281 ccaaaggaga atcttcggtc gatgaatctt ctttgacagg tgagtcccgc ccggaaacca
     5341 agaaacctgg agatcagatt atgtcagggt ctttaaatgg agacggatcg atccaaatga
     5401 tcgctgaaaa acgtgccgca gacagccagt atcaaatgat tattaaacta gtcgaagaat
     5461 caaaagaaaa gcctgcccga tttgtccgtt tagctgatcg ttatgcagtc cctttcacgt
     5521 tgattgctta tttgatcggt ggtatttctt ggtggttctc aaaagaccct ttacgatttg
     5581 ctgaggtact ggtagtggca tcaccttgtc cactgattct agctgcgccc attgctttag
     5641 ttgcaggaat gagccgttca agtaaaaacg ggatcgttgt aaaaactgga acaacgattg
     5701 aaaagctcgc acgtacaaaa acgattgctt ttgataaaac aggaacgctt accaaaggga
     5761 tattggaagt agaggcgatt cagccagtca atggaataaa aaaagaagag ctacttctgt
     5821 atgcagctag tgcagaacag gaatctagcc atatcttagc tcgttcgtta gtgaacaacg
     5881 tgaaaaacag ccaactgtat cctgttagtc aactgaaaga agtgaccggt caaggaatcc
     5941 aagcagtagt caataatcat gaagtaaaga ttggacgtgc ctcttttgtt tcagctgaag
     6001 aaatagaaac gtccaataca gcaatctatg taacaattga tcgatctttt gcaggaacga
     6061 tcctattttc ggatcagatt cgcccagaag cagcaaagac aatcaaggag ctaagaaaac
     6121 ttgatgtgaa aaatttgatg atgatcaccg gggatggatc tgccattgct caaacgattg
     6181 cagaaaaagt ccaattagat tctgtacatg cccgtagctt gcctcaagat aaactgacca
     6241 ttttagaaag tatccctaaa gatcaaagac ctgttgcgat ggtaggagac ggcgtgaatg
     6301 acgcaccagc acttgctgct gcagatatcg gtattgcaat gggggctcat ggttctacag
     6361 ctgctagtga aagtgcagat gcggtcatct tgaaagatga tctcttgcgt gtagcaaaag
     6421 cagtagtgat ttcaagagat acgatgagaa tagcgaaaca gtctgtactg attggcatct
     6481 ttatttgtat ttttttgatg ttaattgcaa gtacgggact aatccccgca ttattcggag
     6541 ctgttttgca agaagtcgtg gatacggtct ctattttgag tgccttgaga gcaaaaaaag
     6601 atccagccat ttttcctgat tcagtaccca agacaaacat agtatgatag aatcgagttg
     6661 aaaaaagaac gatcagaaag aggagagagc attatgccat tcgtacatgt cgaattagta
     6721 gaaggtcgca gtccggaaca attagaaaat atgatgaaag atatcacgga agctgttcat
     6781 aaaaacacgc aggcaccaaa agaacacatc catgtcatca tcaatgagat gaaaaaagga
     6841 acatacggtg taaatgggga atggaaaaaa taagtttagt ttcccacttg atttttctat
     6901 ttattagtgg ttaaataaac acataattga ttgatgagaa agccaactga atttgagagt
     6961 cttcgataag agaggaaaag ctaggctgaa acttttccag gagaaaacca aaggatgacc
     7021 actttcgaaa cccttgttga agcaagggcg gtcggaccgt tacaacgctt gaggaacgca
     7081 tttgcgtttg aacattaggt ggaaccacga acttttcgtc ctagtatctc ttttcaagag
     7141 atgctaggat ttttttgttt ttcagcttga tgatctgatc aatcaaaaca tttcaaggag
     7201 gaaactatta tgataaaaat tacttttcca gatggtgcag tcaaagaatt tgagtctgga
     7261 acaacaacat tggcaattgc tgaaagcatt tcaaaaagct tagcaaaaaa agctttagct
     7321 ggtaaagtga acggaaaact cgttgatttg actcgtccaa tcgaggaaga tgcaagtatt
     7381 gagatcatca caccagacca tgaagatgca cttggcttag tccgtcattc agcagctcat
     7441 ttgatggccc aagccatgcg tcgcctctat ccaaatatcc actttggcgt tggaccagct
     7501 atcgattcag ggttttacta cgatacagat aatggacaaa accaagtaac tgcagaagat
     7561 ttgccggcaa tcgaagcaga aatgatgaaa atcgtcaaag aaaatctgcc gatcgaacgc
     7621 cgtgttcttt caaaacagga agcattagaa atttttgcaa gtgatccgta caaagtagaa
     7681 ctgattagtg aattgccaga agaagaagtg attactgctt atcaacaagg cgaattcatc
     7741 gatttgtgtc gtggaccaca tgttccatcc actggccgta tacaagtatt taaattatta
     7801 tcagtggcag gtgcttattg gcgtggaaac tcaaacaatc aaatgatgca acgtgtatac
     7861 gggacagcct tttttgacaa gaaagctttg aaagaatata tccgtctccg tgaagaagca
     7921 aaagaaagag accatcgtaa actaggaaaa gaacttgact tgttcatggt ttccccagaa
     7981 gtaggttctg gtttgccatt ctggcttcca aaaggggcaa caatccgacg cacgatcgaa
     8041 cgttatatcg tagacaaaga agttagtcta ggttaccaac atgtatatac accaataatg
     8101 ggagatgttg agttgtacaa aacatctgga cactgggatc attatcaaga agacatgttc
     8161 ccgccaatgg atatgggaga tggcgaaatg cttgttcttc gcccaatgaa ctgccctcat
     8221 cacatgatgg tctacaaaaa cacgatccat tcttatcgtg aattaccaat tcgtatcgct
     8281 gaattaggaa tgatgcatcg ctatgaaaaa tcaggtgctt tgtcaggttt acaacgtgta
     8341 cgagaaatga cgttgaatga tggtcataca tttgttcgtc ctgatcaaat caaagatgaa
     8401 tttaaacgta cgcttgaatt gatggtggca gtatatgctg acttcaatat tacagattat
     8461 cgtttccgct tgagctatcg cgatccaaac aatacagata aatattttga tgatgacgca
     8521 atgtgggaaa aagcccaagc aatgctgaaa gccgctatgg atgaattaga actagattac
     8581 tttgaagcag aaggcgaggc agccttttac ggaccaaaac tagatgttca agtcaaaaca
     8641 gctcttggta cagaagaaac attatcaact atacaattag acttcttatt gccagagcgc
     8701 tttgatttga catatgttgg agaagacggg gaaaatacgc atcgtccagt agttatccac
     8761 cgcggaattg tttctacaat ggaacgtttt gtcgcttatt taacagaagt atacaaaggc
     8821 gcttttccaa catggttggc acctatacaa gcaacaatca tccctgtttc tgttgatgca
     8881 catggcgatt atgcttatga aatcaaagaa cgtttgcaaa tgaaggggtt gcgtgtagaa
     8941 gttgatgatc gtaatgaaaa aatgggctat aagatacgtg cctctcaaac acaaaagatc
     9001 ccttaccaat tagttgttgg agacaaggaa ttggaagatg caacagtgaa cgtacgtcgt
     9061 tatggaagta aagaaacagc tgtagaagat ttgaatatct ttattgatgc tatggaagca
     9121 gaagtcaaaa attacagcag agaaaactaa taggtaaatt tcctgtaaaa aagtagtaga
     9181 tagaaagcaa tccgaacaat agataacagg tattcctcaa agacacagat gctcaataga
     9241 atctgtggga ttgatgggat aagatattct gttgggcgga ttgtttttat gttttttatc
     9301 cagtatacga ccgtgaataa gggtctgata tggtatactt acatagacta tcattaaaag
     9361 ggtggaggaa cttatgagcg taattagtcg tattgaaagt atgatggaag attattcttc
     9421 agcagaaaaa aagttggcca actacattat cggtaatatt gagaaggtcc ctacaatgac
     9481 agctaatgaa ttagcagaag cttccgggtt gagtgcgccg acagttgtac gtttttccaa
     9541 aaaaatcggt tttcaaagtc tgacggactt caaaatcacg atttcaacag aactacagac
     9601 tggaatcgat gaaggtttta gtgatataga agcaaatgag tccttttact ctatcaaaaa
     9661 caaacttggt aacaatgcac aagtagcgat aaaagaaaca gtagatattc tagaagaaga
     9721 aacgatccaa aaagtcgtag agtgtttgga atcggcagag actgtttttt tatatggggt
     9781 tggcgcctct tctttagtag tagaagatat tctgcaaaaa tggtctcgtg tcggtaagcc
     9841 aatcattttt gaaaaagata tccacgtctt gctcccgcaa cttgtcagca atgagaaaaa
     9901 gaaagtgctt tggcttgttt caaactcagg tagaagtgca gatgtagtcg cattagccga
     9961 gctggcgaaa agcatgaata tcgaaatcat tgcgttgacg caatttggaa ataacccgct
    10021 tagcaagata gccgatgtac tcgtacaaac atctagacca aaagaaatca cgaatcgaag
    10081 tgcggcaact aattcacttt tggcccaatt tgctacgatc gatatcattt tttatttcta
    10141 tatggctaaa aacgaaaagc ttagcgaaaa agtgaaaaaa acacgagaag caatccaaga
    10201 atattttatg ga
//
LOCUS       DAXWLF010000096        10198 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXWLF010000096 DAXWLF010000000
VERSION     DAXWLF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.096"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            151..300
                     /locus_tag="ACGE4U_001819"
     CDS             151..300
                     /locus_tag="ACGE4U_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFF8393956.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            311..1825
                     /gene="dltA"
                     /locus_tag="ACGE4U_001820"
     CDS             311..1825
                     /gene="dltA"
                     /locus_tag="ACGE4U_001820"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFF8393957.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1822..3027
                     /gene="dltB"
                     /locus_tag="ACGE4U_001821"
     CDS             1822..3027
                     /gene="dltB"
                     /locus_tag="ACGE4U_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFF8393958.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKLFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3072..3305
                     /gene="dltC"
                     /locus_tag="ACGE4U_001822"
     CDS             3072..3305
                     /gene="dltC"
                     /locus_tag="ACGE4U_001822"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFF8393959.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3308..4576
                     /gene="dltD"
                     /locus_tag="ACGE4U_001823"
     CDS             3308..4576
                     /gene="dltD"
                     /locus_tag="ACGE4U_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFF8393960.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4717..5799
                     /locus_tag="ACGE4U_001824"
     CDS             4717..5799
                     /locus_tag="ACGE4U_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFF8393961.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5799..6602
                     /locus_tag="ACGE4U_001825"
     CDS             5799..6602
                     /locus_tag="ACGE4U_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFF8393962.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6672..7412
                     /locus_tag="ACGE4U_001826"
     CDS             6672..7412
                     /locus_tag="ACGE4U_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8393963.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7423..8427
                     /locus_tag="ACGE4U_001827"
     CDS             7423..8427
                     /locus_tag="ACGE4U_001827"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFF8393964.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8472..10196)
                     /locus_tag="ACGE4U_001828"
     CDS             complement(8472..10196)
                     /locus_tag="ACGE4U_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393965.1"
                     /translation="MFSPADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDE
                     TFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPA
                     NYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGT
                     FFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIIL
                     TSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVI
                     STLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYI
                     IILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQ
                     EMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNI
                     QITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVI
                     CCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQL
                     SRNYPNPV"
BASE COUNT         3264 a         1691 c         2075 g         3168 t
ORIGIN      
        1 aaagaaaaaa gcagtgattt gatgtgctat ttttataaaa gctatgtgac aaaactgtta
       61 gtaaaaaaac ggtttctgta agctagacac atgtttcttt ttgtcaaaat tagttacact
      121 ttgtgtgagg ttatatgagg agggaatcca atgaacaaat ggcgttctaa tccaaaattg
      181 aattattggg gaaaattcat cattcgaaca attttttata gtgcagtact gctgttcctg
      241 atttatctgt atcactataa aaatattcaa ggaggaactt ttatctataa cgaattttag
      301 gaggaagagt atggaaatca aaacgattat tgaagcaatc gatgattggg gaataaatga
      361 acctgatcga gttgcttatc aggcggatga gacgcatact tttggtgaat tgaaacaagc
      421 atcagacgca cttgcttatt atttagaaaa aaaggtagaa ggcgatggac caattgtcgt
      481 ttttggcaat ttggagtttg aaatgattgt ctcatttcta ggggttgtca aatctggcca
      541 tgcctatttg ccgattgaag agcatacacc caaggagcgt atcctatcga tctttcgggt
      601 ggctaaacca tctatggtca tcagtatcgg ggactggata gaagatattc cttcggttcc
      661 tgtcataaca aaaaaagaat ttcaagaaat caagaagata ccagtaggat tttatcccag
      721 aaatagcgta aaaggtacag aaaattttta tattattttt acttctggta ctactggaga
      781 accaaaaggc gtacaaatca gtcatgataa tctagtcagc tttgttcaat ggacgatgga
      841 agactttggg atcaagcctg gtatgcattt cttagcccaa gcgccatttt cttttgattt
      901 atcggtattt tctatttatc ctgcattagt ttctggcgga atgctgaagc cgttaaataa
      961 agcagttgtt caagactttc gtcaattatt cgcgacattg ccagccttga agttgaatgt
     1021 gtgggtttct actccatcat ttatggatat ttgcttgatg gaacctacat tcaatggaga
     1081 aaatgtgccg gaattggaca tgtttttatt ttgcggagaa gaattgacga aaaaaactgc
     1141 agaaagtcta cttgatcgat tcccaaatgc acggatatat aatacttacg gtccaacaga
     1201 agcaactgta gctatttcaa gtattcagat tgaccagcat gtactagata gttatgaccg
     1261 actaccgatt ggctacgtaa aaaaagacac gaaagtttct attgtccaag aaggtaaacc
     1321 tgtttcaaaa ggagaaacgg gtgaaatcat tattgcagga ccaagcgtat caaaaggtta
     1381 cctgaacaat cccgaaaaga cagctgctgc gttcttttgt caaaatgact tagcaagtta
     1441 ccatacaggg gatgcaggta gactagatga atatgggctt ttattttatg aaggacgtat
     1501 ggatcagcag gtcaaattac atggataccg gattgaactc ggggatatcg aacattatct
     1561 gctccaagac aaccgaatca aacaagctgt cgtcgtacca aaataccaag gaacaaaagt
     1621 acagcagctg gttgcttttg tcgtattaga aaacaaaacg aaacagccgg attttcagtt
     1681 gactaaatct atcaaagaac aattactaga agttgtaatg gattatatga ttcctcagaa
     1741 gttccattat gtcgatctgc tgcctcagac agttaatgga aaaatcgatc gaaaaaaatt
     1801 aacagctgag gtgaatcctg aatgatgtta cctcatatga tcccttatgc agagccgttt
     1861 tatttttttc ttttagccat tgcgcttgta ccgatcgttc tatctttatt gatatgggaa
     1921 aaaagattac cagtttatca atcagcagtg acacttttct ttttatttgt cagctttgga
     1981 ggtaattatt ggcaacaggg agcggcgctg attgggtatg ttttgtggca gacgatactt
     2041 gtatggggct attttgttta tcggcaaagg aaaaatcaga caggatggtt ttttgccgcc
     2101 gttattttag cgataattcc actgttttta acgaaagtga ctcctttttt tgaacatgga
     2161 aaagcatcgt tacttggttt tttaggaatc tcttatttaa cctttaaatc tgtacaaatg
     2221 atcatggaaa taagagatgg catgatcaaa acatataagc catttcgtta tatccagttt
     2281 ctgctgttct ttccaacaat ctcttctgga ccaatcgatc gctataggag atttgaaaaa
     2341 gatcagctcc atccgcctgt tcgtgataaa tatgttgatc ttttgggaaa aggcgtccaa
     2401 aatatttttg tcggtttttt atataaattc attatcggtt attattttgg tcaagtactg
     2461 cttcctgtag tgggacgcat tgccttacag catggtggta tttcatgggc attagtaggt
     2521 tatatgtacg tgtacagtat gtatttgttt tttgattttg ctggctatag cttatttgca
     2581 gtcggtacaa gctacatgat gggatatcag acaccaatca atttcaataa acttttttta
     2641 tcatggaata tcaaagaatt ctggaatcgt tggcatatga cgctgtcctt ttggttccgt
     2701 gattatgtat atatgcgatt gatgttcttc atgatgaaga aaaaattttt taaaagcaag
     2761 atcgtaatgt caaatatcgg ttattttgct ttgtttcttc taatgggtat atggcatgga
     2821 ttgacgtggt actacatcgt atatggactg tatcatgctt gtctgatctg tctgacagat
     2881 gcatggatcc gatttaagaa gaagcataaa gagcagttgc cttctaacaa atttacgcat
     2941 ggcttagccg tatttttgac atttcaagca gtatgtttta gcttcctgat tttttcagga
     3001 tttttagata aacttttttt tagctaacga atagtgacat attaatttat ataacaaagg
     3061 agaaaaggaa aatggaagaa caaattttat caatcttaga agaaataaca ggaacagatg
     3121 aagtaagaaa aaatcgtgat attgatctat ttgatgaagg gttgcttgat tctttagcat
     3181 ctgttcaatt attagtcgag ctagacggag agctggatat ccaagtccct gtatctgaat
     3241 tcgagcgaga ggattggagc acaccgaata aaatcattca acaagcaact gcattgaagg
     3301 gttgagtatg aaaaagaaac tttttgctat tttcggcccg gttgttttag ctgttggctt
     3361 actgtttgtg ttttttacct cctcctatca agtagatatc tctaatccag aaacgattag
     3421 acgtgcctct tcttccatgt cagtgaacgt gttgaaagga gatgcgataa aaaattatgc
     3481 attatctgaa aaacaatata ttcctttttt tggctcaagt gaattaagca gaatcagtcc
     3541 atttcatccg tctgttctgg cagaaaaata ccagcgaaac tatcgtccat ttttgctggg
     3601 agcacccggg acacaatcgt taagccaata tatgatgatg cgttctgcag gagatgctat
     3661 gaaaaacaag aaagtcgtct ttattatttc tcctcagtgg ttcgttaaaa atggtgtaaa
     3721 aactgattac tttaatacgt attattctga acttcaaaca tacgactggt tgttttcaat
     3781 gaagaaagta acgcctgctg atcgctattt agctagaaga ttattaactt tctcaaaagt
     3841 aaaagagaat gatacgttga cggcgattct gcaaacgatc aaaaaaggga agctaccttt
     3901 acctgaatct ttgaatcagc tgcgcagcca atggaacatg ctgaagagag aggatgaagt
     3961 attcagcaat attgggttga aagatcgaca gcaaaaaatc gatcatgagt caaaacgatt
     4021 accaaaacaa taccaagaaa cagaattatc cattttagca aatcaaattg gagaacgaga
     4081 aacgacgaat aatccattcg ggttgaaaaa cgatttttat acgcatcgta tccgagccca
     4141 cgagccagag ctcaaacagt cacaaaaaaa ttgggattat cgtttttcgc cagaattttc
     4201 tgattttcag ctggtcttgg atcaattagc aaaaaaccat aacgaagtat tgttcatcat
     4261 cccaccggtt aacgagaaat ggagcgatta taccggacta tctcaagaaa tgttacaagg
     4321 atttgccaaa aagatcaaat ttcaattaaa tagccaagga ttcaatcgaa tagctgattt
     4381 tgtcaatcaa gcgggtacca actattttat ggaagacacg atccatctag gctggaaagg
     4441 ctggttggct gcagatcaac agatcagacc gtttttagaa gaaaatcata ttactgctag
     4501 taagtatcat cttgacgatg ctttttttag caaatcttgg caacatcaaa taccagataa
     4561 actacaatta aaataaagaa aacaggcgag tgttgctcgc ttgttttctt tatttttgaa
     4621 tattctgaat aaaagataaa aataccaagt ttgcctgaac aaaaatcata agcaatggta
     4681 taatcacaat atgaatatag aaatggggga aaacatttgg atacaaaaga agaacagcta
     4741 cttattgatt taacttctgc aaaaggagta cctggaaatg aagaagaggt aagagaagta
     4801 ttcagagaat atgccaaacc attcgctgat gatatttttt atgacggttt aggcagtgtg
     4861 attgccaaac atggtgctgg cggaggcccc aaggtcttta tttctggaca tatggacgaa
     4921 gttggtttta tggtaacaaa aataaccgaa aaaggattct tggaattcca aactcttgga
     4981 ggttggtggg ggcaagtcat gcttgctcag caagttgaaa tcaaaacacg tgaagggaaa
     5041 atcgtccacg gtgtgattgg ttccaaacca ccacatgttt taacacctca agtaagaaat
     5101 aaaccttatg aaataaagga tatgttcatt gatattggcg catcaagcca agaagaagcc
     5161 aaagagtggg gcatacgtcc tggagatatg gtcactccat atattgaata caaacgaatg
     5221 aatggcagta aatatttact ggcaaaagca tgggataatc gtatcgggac agctgtttct
     5281 ctgcgagtat tagagaatct atctaaagaa gcacacccaa atgttttatt cgcaggaagc
     5341 gatgtgcaag aagaagtagg cttgcgaggt gctcgaacaa gtacacacct tgtaaatccc
     5401 gatattgctt ttgctttaga tactggtaca gcaggagaca ctccaggaat gacaccaaaa
     5461 gaagcagatt cgattttagg aaaaggccca cagattttga tttttgatgc atctatgatc
     5521 ccgcataaaa aattattgaa ttttgttatt gatgtagcag aggaaatgga tattccattc
     5581 caatatacag tgatttctgg cggaggaact gatgcaggac aaatgcattt gacaagagat
     5641 ggtgttccgt ctgtagccat taccgttcct gttcgttatc tgcattccca tacatcagtg
     5701 attcatgaag atgattattt gaatacagtg aaactagtca cagaattagt gaaacggctt
     5761 gatagtgaaa cagttgaaaa aattcggagt tatgaataat gaataataga atacctattt
     5821 ctgaatatcg gaaaaaagca cgagtttctt tggaaaatca atggggaatc aatacttggc
     5881 ttgttttctt atctataata tttacgagcg tcatccagaa cttttttgac ggtttcagtc
     5941 cttttccgga agaaagcatg cagcagcact tattgagttt tctagtcgat aatttacttt
     6001 tattcgcatt caactatgca ttgttttaca ttgcgctagt agttgtacgg ggtggcagac
     6061 caaaaagtaa ccttttattt tcagttttcc aaagaaagta ttttggttcg atacttggca
     6121 tcaatttgat caattcagtc atcaactggc tactcaacag tttaatcttt ctgcccatgt
     6181 ttttaatcag cggagtgagt acttatatgc agctgctttt cagttcaaat gcggtaccta
     6241 cgggtgtcat gccagaagat tttcttgata tcagctttgt tttatcgatg atagggatga
     6301 tttttgtttc agcactcatc atgagtattg ttggcggttt attccaattt gctgcatgga
     6361 caaagttcga ctatcctgat ttgtcggtta ttcaatgttt gaaatatggc tggtttttat
     6421 tgaaagatag aattggtacg tatattttgt tgcagctgtc ttttatcggc tggtatttgc
     6481 taggagcaat cgctttgttc atcggtcttt tgtgggtcat tgtttatgtg aatgtgtcag
     6541 ttgcagaatt ttatgaacaa gcacggattg aaaaaggagg tccagacgcc tattttactt
     6601 agcatgtaag cagtttattc atcaaaaaaa taacttttgt ggtaggataa atctttgagg
     6661 gggaatgtac gatgaaatta actgttttag gatgcttagg agcgtatcct tataaaaatc
     6721 aagggacaac aggttatttg cttcaatctg acgggttcaa tttgttgatc gatgcaggta
     6781 gtgctacgct gatcaaatta caagaacacc ttgatccact tgatctagat gctgtgatcc
     6841 ttagccatta tcatcatgat catattgctg accttggtgt attacagtat tactggcaac
     6901 ttcatcctaa gagaaatccg gagacattgc tttcaattta tggacacaca aaagattcca
     6961 ttcactttga agaattgacg ttggaaaatg tctcaaaagg acatccttat tttgaagctg
     7021 aagaattaaa aattggtcct tttctgatta cttttatgga aacaatccat ccagtagtat
     7081 gttacgccat gcgtatcgta gaagaaacaa cggggaaagt attcgtattt acaggtgatt
     7141 caggttattt ggaaagtttc gctgattttg cgaaagatgc tgatttgttt ctagcagata
     7201 cctatctttt tgaaggaaat gaacatcatc atgcccattt cacttcaaaa gaagctggag
     7261 aacttgcgaa atcagctaac gtaaagaaac ttgtacttac acacctacct cagtttggtg
     7321 atttagaaca attgaaaaat gaagcgcaaa aggcagcagg caaagggatt gatatagaac
     7381 ttgctgcagt tgacaaagtg tttaacattt aggaggaaga acatgatttt tgtaccaaat
     7441 gaaaaccacg acccaagaat caatttagca attgaacaat atttgctgca agaaatgcct
     7501 ttagatgaac ctattttgct cttttatatc aatgaacctt caatcatcat cggcagaaac
     7561 caaaatacgc ttgaagaaat caatcgagat tatgtggaag aaaatggcat tcatgttgtc
     7621 cgtcgcttaa gtggaggcgg agctgtctac catgattttg gaaacctgaa ctttagtttt
     7681 atcatgcctg atgatgggga ttcattccgt gactttgcga aagtaacaca gccaatcatc
     7741 caagcacttc atgaattggg tgttgctggg gcggaattga aaggccgaaa tgatctagtc
     7801 attgatggca tgaaattttc tggcaacgcg atgtatgcta ccaacggtcg gatgtttgct
     7861 catggaacaa ttatgttcga ctctgatatc aatgaagtag tcaatgcatt aaaagtaaaa
     7921 aaagacaaaa tcgaatcaaa aggaatcaaa tcgatccgtt ctcgtgtaac gaatatcaaa
     7981 ccatttcttc cagaagaaaa gcaaaaaatg acaacagaag aatttcgtca agaaatcttg
     8041 ttgaaaattt tcggcgtaga gtcagtagaa gaagtaaaaa cgtacgaatt gactgatgaa
     8101 gattggaaaa aaatcaatcg aatctctgag gaatattatc gaaattggga ttggaattac
     8161 gggaagtctc cagatttcaa tttctctcgc caaaaacgtt tttctatcgg ttcgattgaa
     8221 gtccatttga atgtctcaga aggaatgatc aaagaagcaa aaatctttgg cgatttcttc
     8281 ggactaggag atatctctga cgtggaaaaa aaattggttg gacagaaata tgataaagca
     8341 tctctatcaa aaatagtcag ccaaattgat gtgaagaaat attttggtgc gatagaacca
     8401 gaagaattgc ttaccttact ttattgaaaa taaaaaatag cggaatccac atatatggat
     8461 tccgctattt tttatacagg attcgggtaa tttcgagaga gttgaacagt gatccgatca
     8521 atgaaagtat ctttttcctg cgttagtgtt cctgtgtaac gatccgctac gtctttcatc
     8581 atcattaagt caaaacgttc ctgatcatct aattcttctg tgatgcctcg actaaatagg
     8641 tttgtatcgt aatgtgcact atgttcgcaa caaatgacga aatgttcctc ttgttcgtgt
     8701 atcgacaaat aaatttttcg atacagtgga ttttcgatgg catgagtttc tctaaaggaa
     8761 tgctctaaag cgtggatcaa aagttgagcc agatcgtcat cgttgatagg aagttcttcg
     8821 ggaagatctg cttggaaggt tatctgaata ttccgtttgg cagctaactt ctggtaacgc
     8881 gctaagataa gatttgtcac ctggtggttg caaaaagaac acagcatttc gaaatttttt
     8941 gcttcttcta atatcttttc cacatagttt ttagcttttg tttggtccgt atgcattaaa
     9001 tcttgcagat tctccatctc ttggacaaat tccaggcgaa gtgtatttac ttcttggata
     9061 tgctgtcgaa gctgctcata atgttgctcc aacaaatcat ttttgatttt taagaaatct
     9121 acttgtctcc gatctttgtc ggtatttaag atgtgatcga ttagattgta gctgataata
     9181 acgattagaa tgataatata taaaatcgta gaccaattga tcgttgtttg atagatacct
     9241 aatgccgttt ggatgtaaat caagcaatgg ataatggcta ccaagacgat ccaaggtgta
     9301 caagctacat aaaaacggtt attcttatat gcttcgccaa tgcatgcaag tgaagtgact
     9361 aaagtgctta taacactagc aatattagca atcggcatta attcaggaag ttctgcagga
     9421 gtaaaagttg cataaagcag aacgccgata aaaatcaaaa gttggaacgt acaaaaaatt
     9481 ccgtaccatt ttttatagta aaacattcgg ctccaatagt agcagatgat gaaagctgac
     9541 gttaagataa taaataaatg agataaaaaa gaatgaacga atggttcaaa aagaatacca
     9601 cttaaaatat cttctgatac agcttcaaat cctaatgcca aagcaaagca acttaagaga
     9661 ataagtgagt attcaagacg tttttgttta tacatcaagt agatcgtata gaaaaaggtc
     9721 cctatagcga taaaaacagc gattatcatc gtcaatactt gcggcactga ttgtgaataa
     9781 ataaaactaa tcaatggtcc agttgttcct agataaactt tcggtggcag tccagcatag
     9841 ttctgataag gagatgagac tgtaagggtc aatttttttc cgatataatt tgctggtaaa
     9901 tctatcacag ccaatgagag tccgggattg ctttcttcct gataatcttt ccgttggtat
     9961 aaaatctttt cgcctagata tacagtaatc cattgatggt ttcctttgat tagtaaattt
    10021 gcttcgtcca acggtttttc aagtgtcgtt tccataatga atgtttcgtc tttatccacg
    10081 gtaggaatcg tacgagtgta cttgctgtca aacttaaaat caggatcatc ttttgtatag
    10141 aaaacccacg aatcttttat ttctttcatt tgattgctgt ccgcaggaga aaaaataa
//
LOCUS       DAXWLF010000097        10092 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXWLF010000097 DAXWLF010000000
VERSION     DAXWLF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.097"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(244..756)
                     /locus_tag="ACGE4U_001829"
     CDS             complement(244..756)
                     /locus_tag="ACGE4U_001829"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFF8393966.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(770..3067)
                     /gene="recJ"
                     /locus_tag="ACGE4U_001830"
     CDS             complement(770..3067)
                     /gene="recJ"
                     /locus_tag="ACGE4U_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFF8393967.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3209..3652)
                     /locus_tag="ACGE4U_001831"
     CDS             complement(3209..3652)
                     /locus_tag="ACGE4U_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFF8393968.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3653..4441)
                     /locus_tag="ACGE4U_001832"
     CDS             complement(3653..4441)
                     /locus_tag="ACGE4U_001832"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8393969.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4438..5379)
                     /gene="rnz"
                     /locus_tag="ACGE4U_001833"
     CDS             complement(4438..5379)
                     /gene="rnz"
                     /locus_tag="ACGE4U_001833"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFF8393970.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5556..6143)
                     /locus_tag="ACGE4U_001834"
     CDS             complement(5556..6143)
                     /locus_tag="ACGE4U_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFF8393971.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6364..7677)
                     /gene="obgE"
                     /locus_tag="ACGE4U_001835"
     CDS             complement(6364..7677)
                     /gene="obgE"
                     /locus_tag="ACGE4U_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFF8393972.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7974..8984
                     /gene="gap"
                     /locus_tag="ACGE4U_001836"
     CDS             7974..8984
                     /gene="gap"
                     /locus_tag="ACGE4U_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFF8393973.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9069..10004)
                     /locus_tag="ACGE4U_001837"
     CDS             complement(9069..10004)
                     /locus_tag="ACGE4U_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8393974.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2781 a         2298 c         1747 g         3266 t
ORIGIN      
        1 tacgggggtg tgacaaaaat cggtcacatc ctttttttcg aataaacggt gttctaaagc
       61 cagtttttcg gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct
      121 ttgcttgttt ccatttataa aaaaatagcg accctcaaaa gtcagataga gaatctgact
      181 tttgagggtc gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc
      241 tgcttaatat tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag
      301 atccatcaat tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag
      361 ttcgatcgtc gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg
      421 ttgacctggt ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc
      481 ataagtcact tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc
      541 gactcctaac tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac
      601 taccatatca attcgttttt cttttgcata atttacgatt tgtttagtag cttcccgata
      661 agcgtcacca tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata
      721 gtcgggaata ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta
      781 accatttctt tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat
      841 tgagccgtcg ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt
      901 tttgtaaaac accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa
      961 aaattaataa tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta
     1021 tatcaattcg ttcttgagaa tggatcaatt taaacaattt tgcgtactgt tctcttgtgc
     1081 ctaccccatt caaataggcc tcatcaggtg aaatccccat cagataaaca cgattgaatg
     1141 aataagaccg aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataacaacta
     1201 gagagtttgg ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa
     1261 tcgcagcttc ctggataatc tcaggcacga atttcaacgt cgaagaatca aatgctaaat
     1321 aaaggctacc ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata
     1381 cttgcagacc acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc
     1441 attcattgat cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt
     1501 gagtaccaaa accaaaagcg accgcatcaa gctgactcga tgcatcggaa aggacaaatt
     1561 tcagatgctg ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata
     1621 aaaactgagg caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc
     1681 gctctactgt tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca
     1741 tcaagtcaat ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat
     1801 tttcacttgg catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat
     1861 ctcgcatctg gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt
     1921 tagctgatcc atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta
     1981 ttttcccagc aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga
     2041 tatgattctc cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt
     2101 ttctttcttc attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa
     2161 acgtcgcaag taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc
     2221 tgggagcaat cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt
     2281 cttcaaatag cgcctgaagt cctatccgtt ctgaatgatg aatggcatcg attcctaatg
     2341 aaacaaggat tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta
     2401 ctaaatccaa aaattctgca ggcggttctt ccaataatgc agaagctact ttgaatgcta
     2461 ctccgactcc tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa
     2521 tcgcataagc gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta
     2581 caccagcttg ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga
     2641 tgatcaactg gattccttct tcgatctttt cttggtatac cgattggttt ggtccatacc
     2701 catgctcaaa tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt
     2761 ctttcattac cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt
     2821 tttctccact gattacagct tcttggattc ggctgattgc tttatccata tcgtgtatca
     2881 gatacggatc atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct
     2941 catggtaacc tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga
     3001 tcatttggag aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt
     3061 gtctcacttt ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca
     3121 gaaagaaaga ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt
     3181 ttgtacgacc atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg
     3241 attgagtttt ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta
     3301 actccgcctg ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt
     3361 cttcttttgc ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag
     3421 tcagctgttt gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa
     3481 tcagtgcacc aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag
     3541 tgaagccaaa acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca
     3601 aaactaaaac aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt
     3661 tgttaaaaat tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg
     3721 atgcaacttc taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga
     3781 cgatcatttt tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc
     3841 ttccgcttgg atcagcctta tcgaagaaat ttgtctcgat tggacctgga ttcactgtcg
     3901 taacagctac gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg
     3961 caaatttcgt ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagagg
     4021 cgatattaat gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca
     4081 aaaccatcat tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg
     4141 taaggaagtc ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga
     4201 cttcttccat caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct
     4261 tgaaagcata agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa
     4321 atgccattct tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga
     4381 tttgtgcgcc cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca
     4441 tgcctcttcc tcctcgcgaa atggaatctc aatgctatca aaatctttca taatcgtcgt
     4501 gttttcaaac acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct
     4561 cgcactgata tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc
     4621 ttgatgagtc gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt
     4681 gctttcatgt accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct
     4741 cgtatcgcct aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc
     4801 attgatcgtc cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag
     4861 tggtccagct ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc
     4921 atgttcaacc acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac
     4981 agaaaattgc tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg
     5041 ataacttaaa cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt
     5101 tggcccgtaa atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc
     5161 gggaagtcca aaaatatgat cgccgtgtaa atgtgtaatg aaaattttac cgatttttct
     5221 cggacgaata gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca
     5281 aacttcattg cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc
     5341 aggaacacct gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa
     5401 gttcctcttt aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt
     5461 caaattaaaa ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag
     5521 cagcgaaccc gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca
     5581 ttgtcgttgg aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca
     5641 tgattttcag cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcga
     5701 gtaatttctc cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca
     5761 tcagcaatca agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac
     5821 acggtctggg catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca
     5881 aaccatccta ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat
     5941 ccacccgtag aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga
     6001 tgtgtcccat taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca
     6061 gcatgtaact ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt
     6121 aaaatgatta ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt
     6181 tacctttaat tgaaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg
     6241 tgacaaaagt cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac
     6301 ttctgtcaca acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct
     6361 tgcctattcg acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc
     6421 accgcgtgcc cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat
     6481 cactgtttca tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga
     6541 taagatccac gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa
     6601 tccatagtgt acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac
     6661 ttctaacaaa tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga
     6721 aattggaaat actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc
     6781 tttgaatttc actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat
     6841 ttgcggacgc tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata
     6901 gtcttcataa ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg
     6961 tgttctttcg atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc
     7021 aatcaagcct ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc
     7081 tagattaggt acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat
     7141 aacggatagt aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa
     7201 tactttcaat tctagttcga tttttcgctc ttgtcctggt tcaccgtttt cagcaatttc
     7261 cggtgcagga ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt
     7321 tgccacaact agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc
     7381 atctcggaca gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg
     7441 catcccttta ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa
     7501 atccatcaat gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc
     7561 gtctccgcca gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc
     7621 gtctccgccc ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat
     7681 taagtcctcc tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag
     7741 tcttacttat tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt
     7801 accttcttct ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata
     7861 attttttgac aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattgt
     7921 attaattgag gatgccgttt cacagcaccc ctaataaagg aggaactttt gatatgacag
     7981 taaaagtagg tattaacgga tttgggcgta ttggccgtct ggctttccgc cgcatcaaag
     8041 aagtctcaga tgatattgaa gtagtagcaa tcaacgactt aacaagtcca actatgttag
     8101 cacaattatt gcaatttgac tctactcatg gtacttatcc tggcacagtt actgcaacag
     8161 aagactcaat cgtagtggat ggcgaagcaa ctcgtgttta tgcagaaccc gacgcaagca
     8221 aaatcccttg ggcaaaagaa aacggtgtag atatcgtact agaatgtact ggtttttata
     8281 catctgaaga aaaagcaaaa gcgcatttag atgctggtgt caaacgagta gttatttctg
     8341 ccccagctgg tgctatgaaa accatcgtct ataacgtcaa tgatgataca ttagatgaaa
     8401 acgacaaaat catctcagca ggttcatgta caacaaactg tttagcacct atggcttatt
     8461 tcttaaataa tgaatttggg atcgaagttg gtacaatgac aacagttcat gcctacactt
     8521 ccacacaaat gctcttagat ggacctgtaa aaggcggtaa tttgcgtgct gctcgttcag
     8581 ctgctgacaa tactatccca cattctactg gtgctgcgaa agcaatcggc ttagttatcc
     8641 ctgagctaca aggaaaatta caaggacacg cacaacgtgt tccagtcgtt gatggttcat
     8701 taactgaatt ggtttcagta ttgaaaacaa aagtaactgc tgatcaagta aacgaagcaa
     8761 tcaaaaaaca cacagttgac aatccttcat tcgggtatga tgaccgtcaa atcgtttcta
     8821 gcgatgtcat tggaacaact caaggttcga tctttgaccc aacgcaaaca gaagtaacaa
     8881 ctgctggtga tttccaactt gttaaaactg ttgcatggta tgacaatgag tatggattta
     8941 cttgccaaat gattcgctta ctagaaaaat tcgctaactt gtaatttgat agatcaatcg
     9001 ataaaagaag cctttgactg gatccagtca aaggcttctt tcttttcatc ccctactttt
     9061 aaactcgttc aatgaacata ttggcttaaa aattgttgga ctacttgctg atatttttca
     9121 ggatctgtct tgtatgcttc cgcatgctcg gctcctttta caacatactt ttcttttggt
     9181 cccttcgttg cacgatagac cttatctaac atttcaaatg gtacaaatgt atcttcgtct
     9241 ccatgtataa agagcattgg gcgctggttt ttcttcaatt gttccactgc atttgcttca
     9301 ccgaaaaagt agccagcacg gacttttgtc actagactcg ttacttgcat aagcggaaat
     9361 gctggaagac taaacatatc gtctagctga taagcaagct catcatgaac ggaagaataa
     9421 ccgcaatctt caatgattgc tttcacatta tctggtagtt tttctccgct agtcatcata
     9481 actgttgctg ctcccatgct gacaccgtat agaacgattt cttgcgattt tccattgttt
     9541 tctagcactt tgtttatcca ctgtacatag tctttacgct ctggccatcc aaatccaatg
     9601 taatcccctt cgctttttcc atgtcctctt gcatcaggaa caaggacatt gtaacccata
     9661 tcatgaaaca tctttgcgta attggtcatc gtttcagcgt ttcccatata gccatgagcc
     9721 acaagagctg ttttttcact tttcgtttca gcaggaagat aaatcgctga taatttcaga
     9781 ccgtcttttg aagtgatggt ccaatcgctt cggttttctt ttgaactaaa ccatttatgt
     9841 gcatccgttt ctttggatgt ctgtgttgta ccaggtgtat cttcgtctaa aaagtctttc
     9901 tcagaaggca caacagcata attataaaaa taattccctg caaacactaa cgcaacaatc
     9961 agagctaaag cgacgcctaa aaggattttc atccaaagtt tcataaatca tccatctcat
    10021 ttctcttttt tcatataacc ttgaattagt ataatcaaag aatctctact atgcaatgtt
    10081 catttttata ac
//
LOCUS       DAXWLF010000098        10085 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXWLF010000098 DAXWLF010000000
VERSION     DAXWLF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.098"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(412..636)
                     /locus_tag="ACGE4U_001838"
     CDS             complement(412..636)
                     /locus_tag="ACGE4U_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393975.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            827..3604
                     /locus_tag="ACGE4U_001839"
     CDS             827..3604
                     /locus_tag="ACGE4U_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8393976.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3685..4104
                     /locus_tag="ACGE4U_001840"
     CDS             3685..4104
                     /locus_tag="ACGE4U_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8393977.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4116..4586
                     /locus_tag="ACGE4U_001841"
     CDS             4116..4586
                     /locus_tag="ACGE4U_001841"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8393978.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4603..5382
                     /locus_tag="ACGE4U_001842"
     CDS             4603..5382
                     /locus_tag="ACGE4U_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8393979.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5369..6190
                     /locus_tag="ACGE4U_001843"
     CDS             5369..6190
                     /locus_tag="ACGE4U_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8393980.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6207..7256
                     /locus_tag="ACGE4U_001844"
     CDS             6207..7256
                     /locus_tag="ACGE4U_001844"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8393981.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7269..8273
                     /locus_tag="ACGE4U_001845"
     CDS             7269..8273
                     /locus_tag="ACGE4U_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8393982.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8906..9925
                     /locus_tag="ACGE4U_001846"
     CDS             8906..9925
                     /locus_tag="ACGE4U_001846"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFF8393983.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3385 a         1664 c         2108 g         2928 t
ORIGIN      
        1 caaagaagga gtatgaaaat gagaaaaata tttattcgtt tattgtgggt actatcttag
       61 aggaataaat aatagtggag aaaactatgg tttggcgcag attctatcag gtttgatgga
      121 acgaagacaa cctcccgaca tcaagtcaaa agctacttga gattttgatc tgaaaataaa
      181 aaaagaaaac aaggtaggaa catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa
      241 aactaagagt ggtgtacgta atcaataaaa aggatggtga caacagtacc tctttactct
      301 ctgaaagcat cataactcgt ttttcctcat tatgccaata aattgttgtt ataccacata
      361 taaggatttg taatagctaa aaacagacag gaaagttgaa atctgtgaat cttattttgt
      421 atggaaggat ttatagaacg caaaaatcaa gataaagatg aataaaaatc ctgtttcggc
      481 tatatattgc gtatctacgc caataaaacg agtttgttct gtcatagcca tataaagcaa
      541 aggcaaaaat acaactacag cgagataaga tccgatgatt tctcgaaaag ttttccagga
      601 agtatgtttc ctgtttttat atttatccga tttcattgaa tagtcatagc ctccttattc
      661 tttcatatgt tgaaattcca gtataaaaac tggaaggaac tgtacgtttt ttagaataaa
      721 aattataagg gataagtagg ctgctgtcta taataagata tatttttcaa ttctttatta
      781 aagattttat tgacaaaatg aatccgtttt ctatgatgga ggaggggtga ataagttgtt
      841 gaagaaggaa attttatgtt acttagagaa ccagacagcc tttttcgatt tagaacatat
      901 gaacgaggtc tttacagcac aaaggttagc tgatcatttt ggggtaaaaa gaaacacagt
      961 cagtcattat ttgaaccagt taaatgatga gcaactacta gtcaaaatca attctcgacc
     1021 agtgatttat ttccacaaag aagcttttga aaaacaattt tttaagttac gcacgaattt
     1081 ttatttttct atcgatgatc tcaaagagga acagcctttt ttcgcgcaac caaaagactt
     1141 attctctttt atgatcggat acgacgccag tttgaaagaa agtatcgaac aaatcaaaac
     1201 agctctttac tatccagatg gcggattacc acttttgatt acaggcgaaa gtggcacagg
     1261 taaaagttat ttagttaatt tggtctatca gtattgtctg cttcatgatc tgctagaaga
     1321 ttctgcacca ttcatcactt ttaattgtgc acaatatgca gataatcctg aacttttgac
     1381 tagcaatttg tttggacatg ttaagggagc gtacactggt gcagaagaaa ataaaaaagg
     1441 ggcttttgaa gcagcagatg gtgggatctt gtttttagac gaggtacatc gcttaacacc
     1501 agaaggtcag gaaaaactgt tcacctatct tgatcaaggt gtgatttatc gtatgggaga
     1561 acctaatttg tcacgccgga tcaaaacacg tctctttttt gctacgactg aagagattac
     1621 tagtcatttc ttaacaactt tcatccgaag aatacctatc cgaatcgaac tacctgcact
     1681 taatcagcgt agtcgtaatg aacggctaga actgatttac tctttttttc tcgaagaaca
     1741 aagaaaaatg gggagccctt tgtcagtcag tggaccagct ttgtcacttt taacaagtca
     1801 gaaattatcc ggaaacatag gagagctgaa aaatatcgtc aaagtttcag cagcaaaagc
     1861 atatgctgaa cagcgtgaac agcctgaaat ccatgtcacg atccatcatt tgcaaaaaga
     1921 actacttgcg cagcctatta ctaaaaatac agcacagcaa ggaatctata ttacgcaaga
     1981 ccagacctta gagcaattaa tggagaagca acagcccgaa cagcagcgaa tcgttaaaag
     2041 ttttgagcaa atcctaattg atttcaagaa aaataactgt ctgctatatt cttgcgaagg
     2101 aaaattaaag caagaagtga ttcaattatt tgattttttg ctgtttgaga ccagtagaca
     2161 agaaaaacac gagctgctgg tttattggac acaatctatc cgagagacct ttcgacaaat
     2221 ggaaactgcc tatcaaatca aattcgatgg aaacagtgtc tatgccttga gttactatct
     2281 ctatcagcga agcactatca aatggttacc ggaagaacat gatattatcc aactaatcaa
     2341 agaattagaa caacaagttt ctcagtctta tccatctagt tatcactatg tccaacgaat
     2401 tctggaatta agtaagatca aattagatat tgaagacaca agtatggatc ggattttact
     2461 gacgatctat ctaaaaaaag cgaaatggtc aaaagaaaaa cgactgccaa aagcgatgat
     2521 tgctgctcac ggttatgcaa ctgctagcag tatggcaaat gtagtaaatc gtttattaca
     2581 agaggatcta tttgaatcat ttgatatgcc actagatgtc acaccacagc agattgcttc
     2641 agaaatcctt gattattgcg aaaacagcga tgtttcaaat ggattgatca tattagtaga
     2701 catgggatct ctgaaagaaa tccatcagtt ttttaaaaaa cagttatccg taccgctttt
     2761 gattttgaat aatgtgacga cacctttagc aatcacagta ggggaatgcc tgcaaaaaaa
     2821 tgtatcatta gaagagatag cagaagagac tgttcgacag atccagccag aatggcgact
     2881 gctctatcca gaggaaaaca aacccaaagc actcattact acttgcttca ctggaatcgg
     2941 tacggcggtc cacttaagtg aactgctaga aaaaagctta cctacggctt gtcagttgaa
     3001 aatcatccca tatgaatatc agcaattaaa agataagaaa aacagtgacc cattattttc
     3061 catatacgaa gtcgtaggaa tgattggcac cacagatcct gatatcacca atatcgacta
     3121 tctttcatta gaagacctca tctcggggga aaagatgtct gtactagctg aatggttgga
     3181 cactactatg aatgcttcag agaaggaaat attcaatcag cgcatcattc gtaatttttc
     3241 attggaaaaa gtactggatt ccgtgactat tctagataca gagaaggtga tgggagaaat
     3301 cgatcatttt atgagagaat tagaggtgca gttgaatcga agcatcagca atcccaaaaa
     3361 attagcgttg tatgtccatg tgagctgtct cattgaacga ttgatccgac aaatgccaat
     3421 cgaaacctat caaggattag agaaatggaa gcagtgtcaa aaagaagggt tatctgctat
     3481 taaatctgcg tttagtgtca ttgaagagca ttatagtgtc atgatcccca attctgagat
     3541 tgcctatatc tatgatattt tgagtgaaga tacagagttt ctctcgatag aagaagaatt
     3601 ttgaacataa gcaagttgtt gccactaatt ttggcatgca acttgctttt ttagtatgtg
     3661 taagtgaaaa gaggagatga aaaagtggat agagaaatta tcttagcttc ccatggaaaa
     3721 tttgcttcag ggatattaga ttcattagag ctgatctatg ggaaaaatca ttctattact
     3781 gtattcgatt gctatactga tgagaatttt gatttagcgg aaacagtaag gcagcttttt
     3841 atgaggtaca aagataaaga aatcattgtt ctgacggatc tttttggcgg aagtgtcaac
     3901 aacgaattct tacaatatat caatcgggat catgtctatc tcgttgccgg attgaatttg
     3961 ccactattga ttgagctagt atctcggttg gacgctgaag tagagacaac tgttttgatc
     4021 cagcaggtgc taactgattc aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag
     4081 aaaatagaag aagacgaatt ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc
     4141 atcggttatt acacggacaa gtagcttttt catggacaca aacaataggc gcagactgta
     4201 ttttgattgc taatgatgat gtacctacga atgagatacg aaagacaacg atcaaattag
     4261 ccaagccaca aggagtcaaa ctagtgatca aatcaattga tgactcgatt gccgcgttga
     4321 aaagcggggt gacagataaa tataagttat tcatcgtagt ggaatcgatc gaggatgcat
     4381 ataagctggc agaagcgtat ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa
     4441 aacctggtac tcgcagcatc ggaaaagcgg taaatatttt accagaagag gaggaacgct
     4501 tgaaacgatt aattgacaaa caaatagaag tagaaatccg tcaagtacca gcagataaaa
     4561 aagtggatgt aagagaagtt ttgtaagaaa ggaagaacct caatgattgt acaagcagtc
     4621 ttattaggaa ttgttgcatt tgtcgcacaa tcagaatatg cactagggac atcgttgctt
     4681 tctcgtccga ttgtgacggg gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg
     4741 atcattatgg gcgcaacttt agagttagca tttatcggat cgttctcagt aggtgcttcg
     4801 attccacctg atgttgtgac cggtgggatt ttagggacgg cttttgctat tacagctgga
     4861 gccggtacag aaaccgcgct tttactaggt ttgccgattg ctacgttgac attgatattg
     4921 aaaaatattt acttagggct tttgattcca attatgaacc ataaagcaga caattatgca
     4981 gaagaaggaa attataaagg agtcgagcat atgcacttgc ttgccggtat cggtctttcc
     5041 ttgatgcttg gtgttgtggt aatggtttct tatatggtgg gaagcaatac gatcggaaat
     5101 ctattaaata tgattcctga cttcgttcaa aaggggttgt cagtagctac agggttgatt
     5161 ccagctcttg gttttgcgat gcttgctcgt ttgctgatca acaaacaagt tgcaccatat
     5221 tttttcttag gttttgctat agcttcttac ttagagattc ctgtgaccgg agtagctatt
     5281 tttggtgcga ttttagcagt tgtggtagtc aatattatga atgtccgtca actaccactt
     5341 caaacaactg caggggaggt agaagaagat gaagaattct gagatcaaaa acagcaaaat
     5401 aacgaaaaaa gagctaaatc acgtattttg gcgttctttt caaatggagt tttcttggaa
     5461 ttatgaacgt cagatgaata tggcttatgt ttatgcaatg attccgattt tgaaaaagtt
     5521 atatcagtcg aaagaagaaa tggcagcagc tttgaaacgg catctagagt tttttaacac
     5581 gaccccacat atcgttacac tgattttagg aatcacggct gcaatggagg aagaaaataa
     5641 ggatgatcct gaatttgatg tgactgcgat cgatagtatc aaaacatctt tgatgggacc
     5701 tcttgcaggg ttaggagatt ctttcttttg gggaactttg cgattgattg ccacaggtgt
     5761 gggtacctct ttagccatgc aaggaaatat tttaggccct atcttgttca ttttgatttt
     5821 caatattccg cacattctgt ttcgctatct agcaacaggt tggggatatc gcttaggaac
     5881 gggttttttg aaaaagatcc aggcaaacgg tatgatggaa agtttgacat taggcgcgtc
     5941 tattattgga ctgatggtcg ttggagcaat gactgccacg atgatcacca tcaatattcc
     6001 tttaaaaatc ggttcaggag aaaatgctgt gactgttcaa agtatttttg atgatatcgt
     6061 tccaaatatc ttatgtcttg gagcatttgg catagtgttt tatctattga agaaagaagt
     6121 gaaaccatta acgatcttga tcggactagc tttctttgga atttttggtt cgtggatagg
     6181 gattttttaa aaaaggagca gagaaaatgt ttacgatgca agattatatc tatgaagaaa
     6241 aggaagtact aagtacgatc ttgaaaaaga atgatttttc gacaagagaa catatgaaaa
     6301 aaacagtgaa tttactgatc ttagcaacgg gttcatctta taacgcttgt ttagcagcga
     6361 agccagcatt ggaatcttat ggtgatctta ctgtcgatat tcaagaacct ttttattttt
     6421 tcaattatgg aaaactttct ccttcaatcg atactgtgat tgctgtgtca caaagcggga
     6481 aaagtgcttc tactgttgaa gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg
     6541 ctatcacaaa tgacgtgcaa agtccacttg ctttagaagc tgcacaaatt attgatttag
     6601 gtgccggggt agaatcagta ggcttcgtta caaaaggcta ttctgcgact gttttacagt
     6661 tactactctt agggatagga attggaataa gtaaaaataa aatcagcaaa aaaatagaac
     6721 aagactacat gcaacagttg cgcaaaataa tcaaccattt accagcgatt attcaaaaaa
     6781 cggaagaatt ttttgatgga tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca
     6841 ttggatatgg tccaaactgg ggaacagcta aagaagcaga gacgaaattg actgaaacaa
     6901 tccgcgttcc gtcacaagga tttgagttag aagcttatat gcacggccca tacctggagg
     6961 cagatgcttc gcatttgcta ttctttattg aaggggaaag tgtaaataag gaacgttcgc
     7021 aaaagttgca aagatatatg agtcggtacg ttggagagac tctcaccatt accacaaaaa
     7081 aagcaagaaa cgagaaaaca cttggattag caatagaatg tgatgaatat ctgtctgtat
     7141 tagcgcttgt cgttccgttt caactattcg cttataaaac cgcagtagct aaaggtattg
     7201 acttgaacaa aaagattttt gaagactttg atacagtctt gaaaagtaaa ttataagagg
     7261 tggaaatcat gctgaaattc aatgaagaag aacaaatcaa agcaataaaa ggcgcactag
     7321 ctttacgtcc gcaagtagaa gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg
     7381 tctattatct aggaatcggt ggaacgtatg cttcctcgat gcaagctgtc acttatatga
     7441 atgggaaaag caacttgcct gtctttgtac agcatgctgc agaatattac actactggaa
     7501 acaaaagatt aacaaaagat tctatcgtta ttttgtcctc tgttaccggt acaactcaag
     7561 aagtagtgaa agcagtagaa caaatcaaga aagtaggagc gacactgatt ggatttatcg
     7621 ataaagcgaa cagtaagttg tctcagctat gcgattttgt ggtcacttat ccagctcctg
     7681 gaacagaaca aatcaaattt tttatggcag cagatcgatt gatgtatttg aatggagaat
     7741 tcgaagctta ttcagaatac tatgaacagc tcgaacgtta tttaccaaca gggttagtgg
     7801 aagcagagaa aaaagcagat gcatttggct tatcatttgc agaaaaacat cgacgtgata
     7861 gtatgcatta ttttattgga gcagggaatc aatggggagc tgtttattca tatgccatgt
     7921 gttactggga agaacaaagc tggcttccat cgaaatctat ccatgctgcc gagtttcttc
     7981 acgggacgtt agagatcgta gaagaaacaa ccccagtcac attattttta ggcgaagatg
     8041 aacagagagt gttatctgaa cgtgtggcta aacttttgcc aaaaatttgc agcaattata
     8101 cattgataga cacaaaagac tatccagtag aaggtatcag tgaaaaatat cgcggacgtg
     8161 ttttgtcttt cttattgatg catgtagtga cacaacgcat cgatgctcat gtggagcagt
     8221 tgaactgcca tccattagag atacgccgct actatcgtca gtttgattat taggatagac
     8281 tggataaaaa gaatataaaa aataaagaga gcagcgataa ctgctccctt tattttttta
     8341 aaatattggc atcaattacg ttataaaaca cattttttgc attatctaca gttcaactta
     8401 taatctgttt aatgggacat gtgggtgcca accatttttt gttatgcaat agtactattt
     8461 agttgttgca tggatccgta gtaaaagtcc aagtacaacg agttgcgcca gtttacatag
     8521 cgttttgtat ccagaatgtt gactaaaaca aaagttccta tgtcaaccgt caacagagac
     8581 aatctgtccg ttgcaggacc tgacagattt agaaatgttc aatctgtctc ttatgtattt
     8641 taaatcaata ctcactagga gaagtttcct aacctcctaa tagtaatgct ggtagtaaca
     8701 ttgtcaaaac tgcttcctct taggaacaag caaagcataa cgagtgtaaa aataaaatgt
     8761 agactattgt aacatatcat tgattatttc aagtaaaaac gataaaagaa gtaaacaaac
     8821 acttaaaaac aatggaaatt agtcccaact ttaagaaatg tatactataa tggaaattat
     8881 caattattta tttggaggaa tatccatgaa acaaaaaatc gcagttcttg gtcctggatc
     8941 ttggggcact gctttagcac aaacattggc agaaaacggg catgatgtcc gtatttgggg
     9001 aaatgtacct gaacagattg atgaaatcaa taagtatcat acaaatcagc attttttacc
     9061 tgatttgacg atcccagaat cgattatcgg atataaagaa ttggcagaag cagtggataa
     9121 cgcagatgct attttatttg ttgttccaac aaaagcgatt cgttcagtcg cacaggaact
     9181 tattggtaaa atgaatacta agccaatcat tatacatgca agtaaaggac tagaacaaga
     9241 tacgcataaa cgaatctcag aagttttggc tgaagaaatt ccagaagaaa aaagacaggc
     9301 aattgttgta ttatctggtc ctagccatgc tgaggaagtg gcagtacatg atatcacaac
     9361 aattacagct gcaagtattg atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa
     9421 tgattatttc aggatttata caaataatga tgtgattggc gtagaaactg gtgcagcact
     9481 gaaaaatatc attgcaattg gtgccggcgc tatccatgga ttaggattcg gagatgatgc
     9541 aaaagcagca atcatgactc gaggattggc agaaatcagc cgtcttggtg tcgcgatggg
     9601 tgccaatcct ctgacattta tcggtttgag tggtgtgggg gacttgatcg ttacttgtac
     9661 aagtgttcat tctcgcaatt ggcgtgccgg taacttgtta ggacagggac ataagctgga
     9721 agaagttcta gaaaacatgg gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt
     9781 agagctagct caacagctag gtgttgagat gccaattact caaacaatct ataatgtttt
     9841 gtataatgga gaagatatca aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat
     9901 ggagaatgaa ttttctcttc gttaaataga tgatcaatag ttaatcgtcg tttagtctaa
     9961 tggtgataag tcaagatgaa atttaagatt ttctttattg agtaatatgt tttgaagcgc
    10021 acgaaactag acccgatcaa aatcaaattt ttttcgggtt gattcaagga tttgcgtcaa
    10081 taacg
//
LOCUS       DAXWLF010000099        10043 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXWLF010000099 DAXWLF010000000
VERSION     DAXWLF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.099"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            84..986
                     /gene="gnd"
                     /locus_tag="ACGE4U_001847"
     CDS             84..986
                     /gene="gnd"
                     /locus_tag="ACGE4U_001847"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFF8393984.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            988..1989
                     /locus_tag="ACGE4U_001848"
     CDS             988..1989
                     /locus_tag="ACGE4U_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFF8393985.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1986..2270
                     /locus_tag="ACGE4U_001849"
     CDS             1986..2270
                     /locus_tag="ACGE4U_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFF8393986.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2303..3100
                     /locus_tag="ACGE4U_001850"
     CDS             2303..3100
                     /locus_tag="ACGE4U_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8393987.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3114..3947
                     /locus_tag="ACGE4U_001851"
     CDS             3114..3947
                     /locus_tag="ACGE4U_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8393988.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3965..4822
                     /locus_tag="ACGE4U_001852"
     CDS             3965..4822
                     /locus_tag="ACGE4U_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8393989.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4844..5353
                     /locus_tag="ACGE4U_001853"
     CDS             4844..5353
                     /locus_tag="ACGE4U_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFF8393990.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5390..5791
                     /locus_tag="ACGE4U_001854"
     CDS             5390..5791
                     /locus_tag="ACGE4U_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393991.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5903..6256
                     /locus_tag="ACGE4U_001855"
     CDS             5903..6256
                     /locus_tag="ACGE4U_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393992.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6290..6733
                     /locus_tag="ACGE4U_001856"
     CDS             6290..6733
                     /locus_tag="ACGE4U_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8393993.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6750..7835
                     /gene="uxuA"
                     /locus_tag="ACGE4U_001857"
     CDS             6750..7835
                     /gene="uxuA"
                     /locus_tag="ACGE4U_001857"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFF8393994.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7828..8541
                     /locus_tag="ACGE4U_001858"
     CDS             7828..8541
                     /locus_tag="ACGE4U_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8393995.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8748..8897)
                     /locus_tag="ACGE4U_001859"
     CDS             complement(8748..8897)
                     /locus_tag="ACGE4U_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8393996.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9293..9493
                     /locus_tag="ACGE4U_001860"
     CDS             9293..9493
                     /locus_tag="ACGE4U_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8393997.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3389 a         1557 c         2210 g         2887 t
ORIGIN      
        1 atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg
       61 gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat
      121 tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa
      181 gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat
      241 ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa
      301 ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag
      361 gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa
      421 tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct
      481 taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag
      541 caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga
      601 tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac
      661 aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg
      721 tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa
      781 gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc
      841 tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac
      901 aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac
      961 atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc
     1021 agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt
     1081 catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga
     1141 ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa
     1201 gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca
     1261 gcaaaagaac accagacaat cgtatctatc atacctgctt taattgaacg aaatgctgta
     1321 gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca
     1381 agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac
     1441 aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt
     1501 aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa
     1561 agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt
     1621 ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag
     1681 atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg
     1741 atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa
     1801 gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat
     1861 atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc
     1921 gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata
     1981 aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt
     2041 tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa
     2101 gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt
     2161 tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg
     2221 aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg
     2281 cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg
     2341 agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa
     2401 ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat
     2461 aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga
     2521 tacactagca aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga
     2581 atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca
     2641 ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct
     2701 ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag
     2761 aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg
     2821 ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg
     2881 ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct
     2941 agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat
     3001 tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca
     3061 gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa
     3121 cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct
     3181 ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg
     3241 tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga
     3301 tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag
     3361 ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg
     3421 cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt
     3481 ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat
     3541 caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg
     3601 ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg
     3661 ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg
     3721 tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg
     3781 ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg attattatcg
     3841 ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca
     3901 attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg
     3961 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
     4021 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
     4081 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
     4141 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
     4201 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
     4261 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
     4321 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
     4381 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
     4441 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
     4501 cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga
     4561 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
     4621 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
     4681 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
     4741 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
     4801 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
     4861 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
     4921 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
     4981 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
     5041 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
     5101 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
     5161 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
     5221 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
     5281 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
     5341 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
     5401 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
     5461 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
     5521 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
     5581 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
     5641 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
     5701 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
     5761 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
     5821 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
     5881 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
     5941 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
     6001 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
     6061 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
     6121 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
     6181 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
     6241 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
     6301 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
     6361 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
     6421 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
     6481 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
     6541 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
     6601 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
     6661 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
     6721 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
     6781 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
     6841 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
     6901 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
     6961 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
     7021 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
     7081 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
     7141 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
     7201 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
     7261 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
     7321 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
     7381 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
     7441 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
     7501 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
     7561 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
     7621 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
     7681 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
     7741 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
     7801 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
     7861 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
     7921 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
     7981 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
     8041 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
     8101 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
     8161 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
     8221 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
     8281 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
     8341 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
     8401 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
     8461 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
     8521 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
     8581 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
     8641 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
     8701 cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt
     8761 tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct
     8821 ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct
     8881 ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac
     8941 gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa
     9001 gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat
     9061 caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat
     9121 atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta
     9181 ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg cctatcaaat
     9241 atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa
     9301 cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg
     9361 aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga
     9421 aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat
     9481 tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag acggtttttt
     9541 tgtacgaaaa aattcattgg tcgttgaatg ggctgaatag tagtgtataa aacaattttg
     9601 tggagcgtga agcttttatg gtagaggaaa atacgaataa agtgatgttg ataaaaagat
     9661 tcctatcttg aatatgaatc gagatgtctt gaaaatgaat gagtttgacc aatcattatc
     9721 ctatcgaaat ttacagtttc gttcgattgg tctgtcaatt aatgaaaaag taaaaaagaa
     9781 tatgttctag gaagagggcg gcacatctaa atttttaagt aacggacagt tcaaatatca
     9841 agcattgaaa gaggctatcc tactgtttga aggaatacga atatataaaa acgacaaaga
     9901 ggaaaaagta gttcattaaa tcccttcaag tgatgaaaga atgagatcta ataaaacctg
     9961 atctttcatc tgttttttta ataacgtaat aattttttta ttttttgtgc tttcgccgtt
    10021 attgtagagt gatttagcat tct
//
LOCUS       DAXWLF010000100         9835 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXWLF010000100 DAXWLF010000000
VERSION     DAXWLF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.100"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(78..2198)
                     /locus_tag="ACGE4U_001861"
     CDS             complement(78..2198)
                     /locus_tag="ACGE4U_001861"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8393998.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACGE4U_001862"
     CDS             2428..3606
                     /locus_tag="ACGE4U_001862"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFF8393999.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACGE4U_001863"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACGE4U_001863"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFF8394000.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACGE4U_001864"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACGE4U_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFF8394001.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACGE4U_001865"
     CDS             complement(4959..6212)
                     /locus_tag="ACGE4U_001865"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8394002.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACGE4U_001866"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACGE4U_001866"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFF8394003.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACGE4U_001867"
     CDS             7789..9135
                     /locus_tag="ACGE4U_001867"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFF8394004.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="ACGE4U_001868"
     CDS             complement(9443..9829)
                     /locus_tag="ACGE4U_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8394005.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3141 a         1789 c         2012 g         2893 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaaga
//
LOCUS       DAXWLF010000101         9746 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXWLF010000101 DAXWLF010000000
VERSION     DAXWLF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.101"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(45..1355)
                     /locus_tag="ACGE4U_001869"
     CDS             complement(45..1355)
                     /locus_tag="ACGE4U_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFF8394006.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            complement(1387..2211)
                     /locus_tag="ACGE4U_001870"
     CDS             complement(1387..2211)
                     /locus_tag="ACGE4U_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8394007.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            complement(2227..3123)
                     /locus_tag="ACGE4U_001871"
                     /pseudo
     CDS             complement(2227..3123)
                     /locus_tag="ACGE4U_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(3206..4321)
                     /locus_tag="ACGE4U_001872"
                     /pseudo
     CDS             complement(3206..4321)
                     /locus_tag="ACGE4U_001872"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            complement(4346..4471)
                     /locus_tag="ACGE4U_001873"
     CDS             complement(4346..4471)
                     /locus_tag="ACGE4U_001873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394008.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            complement(4609..5634)
                     /locus_tag="ACGE4U_001874"
     CDS             complement(4609..5634)
                     /locus_tag="ACGE4U_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8394009.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(5879..6712)
                     /locus_tag="ACGE4U_001875"
     CDS             complement(5879..6712)
                     /locus_tag="ACGE4U_001875"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8394010.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            complement(6728..7163)
                     /locus_tag="ACGE4U_001876"
                     /pseudo
     CDS             complement(6728..7163)
                     /locus_tag="ACGE4U_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            complement(7434..8381)
                     /locus_tag="ACGE4U_001877"
                     /pseudo
     CDS             complement(7434..8381)
                     /locus_tag="ACGE4U_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            8393..8866
                     /locus_tag="ACGE4U_001878"
     CDS             8393..8866
                     /locus_tag="ACGE4U_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8394011.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(9039..9593)
                     /locus_tag="ACGE4U_001879"
     CDS             complement(9039..9593)
                     /locus_tag="ACGE4U_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8394012.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         3085 a         1883 c         1450 g         3328 t
ORIGIN      
        1 aaaagagtaa ggaattgaaa attccttact ctttcagctt ctttttaatt atattcaggt
       61 aaatagtccc catgtgcttc gattaaatca tccactaaat ttttgatatc tgttaaagac
      121 aattcagatg atagatgtgg atctaaatac gctgcttgat aaatttttga tttatctagt
      181 gttaaagcgg cttctactgt cagttcttga acattgatat tcgtccgatt taaagcagct
      241 aattgtgttg gcaattcacc gacataggtt ggttgcacgc catttttatc gactaaacaa
      301 ggtacttcca cacaaacatc tgtcggtaag tttgtgatta agcctttatt taagacattt
      361 cctgcaatca ctgacggagt tcctgtcgta atagaatcca tgatatatga agcatattca
      421 cggcttctcg tatgtgttaa ctccccatta tttacaatgt cctctcgcat gctttcccaa
      481 tcattaattt gttgttcaca acgacgtaag tattcatcca acggaatccc taattcgtca
      541 atcaattctg ggtattttga tttaataaag tatggatggt attctgcatt gtgttcactt
      601 gattctgtaa tatagtaacc aaaacgtttc aacaattcaa aacgtacaga atctggatgt
      661 ggatttggtt tttctgcagc taactgacga attgttggat agaaatcttc cccatttttt
      721 gtaatttcca acaagaatgc catatgatta atgcctgcaa ttttccattg aaagtcattt
      781 aagtcatatt gatcggctat tcctaaatgc tcaaacaatt ctggtacgca tacttgaacg
      841 ctatgacata aaccaacagt cttaatactc gtcgctttta aaacacccat ggttaacatc
      901 gccatcggat ttgtatagtt caataaccaa gcatttggac aaacttcttc catttcttta
      961 gcaaagtcta acattactgg tagtgtccgt agggttctaa aaatcccacc aatccccatg
     1021 gtgtcaccaa tagtttgtcg caacccatat tttttaggaa tatcaaaatc aatcactgtt
     1081 gaaggtttat aaccacccac ttgaatagca ttgataacaa aatcagcctc tcttaacgct
     1141 tctttgcgat cataataaga aacaatatca gcacgatttt cattcgaatt tttattaata
     1201 tttttaagca ttaaatttga ttcttttagt cgtttttcat caatatcgta caatgcaata
     1261 tgtgcatctt gtagacttgg agttaacatc gcatcgccta atacgttttt tgcaaaaata
     1321 gtacttcctg caccaataaa tgtaattttt gtcatgtact tactctccaa tcactaatta
     1381 atagttttat aatttactac ctgttgtagc aattccttca atgaattttc tttggaaaac
     1441 gatgaatatc acaactattg gccaaattgc taataacgaa cctgccatta attgtgggaa
     1501 gttcgtggca tattgtcctt gtaagtttgc taatcccgga gctaaaggca tcttatcaat
     1561 tgaagtattg acaatcaatg gccacattaa atctttaaat gcaaacaacg ctgtgaatat
     1621 tccaagtgca accaatcctg atttagcaag cggcaacata attttgaaaa agatcgtcca
     1681 aatattggca ccttctaaat aggccgcttc ctccaattca tcaggtaaac ctaagaaaaa
     1741 ttgtcttaat aaaaacgtac caaacgcact aactacccct ggtaatatta atgctggaat
     1801 tgtatttaaa atacccaatt tttgtgctaa taaatactgt ggagtaataa aaatggaacc
     1861 gggaatcatc atttgaataa gtactaagct gaaaaggata tttttccctg gaaaacttag
     1921 acgcgcacaa gcatatgcag ccattgctga aaataaaacc gagccaatca ctcgtccaac
     1981 aaccattaaa aatgtattga tataaaaagc catgaatggt aataaatcaa gtacttgttg
     2041 atagttactt agctgaaatt tctcagggaa aattgtaggt ggaatttggg tagattcaac
     2101 caacgttttc cctgaagtta aaatcatcca aataaaaggt aatgccattg caataccacc
     2161 taaaataagc atgatatgga ttggcgcatt tgaaaaaaat ttatttttca taatttgata
     2221 gcctccttaa tagtttaccc actttttctg caacttcatt tgaattgcag taataattaa
     2281 tataatcact aaaaggaaca taacgattgc cgaaccatat cccttgtcat ttaaaataaa
     2341 tgaatgttta tagaataaat aggctaatga ttgtgattta aacatggcgg tactattagg
     2401 agtaatcatc ataaaaataa catcaaatac ttggaaagca ttgataactg ttgttacaac
     2461 tacaaaaaat aatgttggtg taactaatgg taacgtaatg ttgaaaaact gctggattgg
     2521 actagcacca tcaatttccg ctgcttcata ataatcttta ggaatttctt ataagccggc
     2581 tagtaataac accatgttat aaccaatagc actccaaact cctacaatga taatagaata
     2641 aatcgcaatc gatggatccg ttaaccatgc tggtccatcg agacctattt tagccaacat
     2701 gttattaatc agtccataat cattattgaa catccagcgc caaatcattg cgactgcgga
     2761 tggtgcagca acgactggta gaaaataaat tgtccgataa atcgacaatc ctttaacttt
     2821 tctattcatt aaaactgcca ccaacaagga taatacaaca atactaggta tcgaaaccac
     2881 tgcataaatt aatgtgttta ataaagactg aaaaacttca ccgtctgcaa acattttttg
     2941 gtaatttctt aatccgaccc attcagtatc accgaaccct accgtttttt gaaaacttaa
     3001 aattgctgtt tgaattgctg gccatatatt taataaaatt aatcctaatg ccgttggaaa
     3061 aataaaaata tagccccata tcacttgatc tttacgatat tttccaaaaa ttcgattttt
     3121 cataatatcc ccctttttgg tattcatcca ttaatataca gacgaaatag aatcctttcg
     3181 cctgtatatt gtataattaa ctaatttaat ttccgctcaa tatcgcattg acttcttttt
     3241 caatttgagc ggttccatct tcaacagtta cttcaccaga cagaatacgg ttaataattt
     3301 gaacttctgc ttgttcagct cgtaatccat ctttattgta tggacgaggg actccatact
     3361 ccaacatttt tccaaaagca ctcaaatcaa ttgtatctga agaagcagcc cactcgtctt
     3421 caaagccttt tagtgctggg attgatgccc ccgtttccgc ttggatttta ttggcgtcct
     3481 cactagctaa aaatgccatt agttttttca cttcttctgg gtattttgta ctcgcactgg
     3541 cagcccaacc aagtccattt gtaattgttg cattaacttt tcccttaggt aacacggcag
     3601 aagcaacatt ttcaactaga tagtcatttt tcaaaaatcc cggtgcaaac cagtttcctt
     3661 caatcatcat tgctaatcta cctgattgaa aacgacttaa cttatcagga tcttgtaatt
     3721 ctgtaattgt tggtgaagca tgatcaacaa gtgcaatatc tcgataaaaa gctaaagctt
     3781 cttgtgcttc tggggtattc aaacctgatt ttgtattgtc atcggcaaat acttctcccc
     3841 cattttgata aataaatggc cagtagccag tctctgttcc aatatgtgat gcaaaaccat
     3901 agatgccttt tgattcatct gttaaatcaa ttgcagcttg tctgagtgtt tcccatgtcc
     3961 atgtttcatc tggataatcg acacctgcat tatcaaataa ttctttgttg taccatagcc
     4021 ccactacaga agcatcttta gggattgcgt atagttcgtc ttcaagcgta tagaaatcag
     4081 caattccttc agtaaagttt cccatgtcaa tatcattttc tttgataaca tcatcaagtc
     4141 ccatcaaagc attctgtgac atatatttat aggattcgct agagtgcatg gttactacat
     4201 ctggcatatt acctcccgtt gttgcagctt ctaatttaac ccaatattga tcccaaggcg
     4261 ttgtctccat ttctactttg atatcaggat tttgtttttc aaattcatct aacatttttc
     4321 ttagtcctgg agcttggtca gtatctcaac tcgcatagct taaagttact tgcccgtctt
     4381 tagaattact atctccccca ccacatgctg cgagtaatgt cccagttaaa acagctatcc
     4441 ctgttaaagc tatttttttg aaatgtttca tcttaattcc tcctgttttg aatgcgtttt
     4501 ctttttgtta acaacaccat aactgtttac ttaatgtttt gtcgagattt tttactaaaa
     4561 atattagtaa acaaaaaagt aaacaccctt ttttggatgt ttactttttt aatcgatatt
     4621 tttttcactt tctctaacaa taatattatt agaaacaatc actttgatcg aaacatttct
     4681 aacttctttt attcgttcat taaccatgcg aactgctagt tttccaattt cttctgtatt
     4741 cacattcacc gtatttaatg caggtgttaa aaattcagca acttcaatat tatcaaaact
     4801 cacaatcgaa atatcttctg gaatatgtag accatttttt tgaagtgaac gataaacccc
     4861 cacagcaata ggatcatttc ctaccatgat agcagtcggc aacttatctt tagcatgttt
     4921 tttcaaaagt aaatctgctg cctgcattcc gtcaagtgtg gtccagcctt ctaaataaat
     4981 ttgactatat ttttctaatc ctttttgttt catccacgtc tcataggcta tttttcgata
     5041 atcatcatct gacacgtgtt tatttccttg gctatctaaa cgaacacgtc ctcctccgat
     5101 aaacgcaatg tttcggtgtc cttttttata caatcgatct aaatgctgat tcgttgcttt
     5161 tgttaaatca gtataaactg catctacttc agcttcaata ctagggtcat caatcactac
     5221 gacattcgaa ttaattgctg atacttcttc aatcacagaa gatagcacct gcccaataat
     5281 caacacacct tgacatttta gcaaatcgtc ttgctccaac ggtgtttctg acaaacgaat
     5341 aattttcttc aaattgactt tgctttgttc tgcttcgact tgaatccctc tgcgaatcgt
     5401 tcgaaaatag ggatcttcca actcatctac ttcagaaact gtcgttatta atgcaatatt
     5461 tgttaaattt tttgcaacgg gacttttttt gacataatga taaaaattaa ccgattctgc
     5521 aatctttcgt ttggtttcat cggttactga aagtgtcgga tcctcattta gcacgcgcga
     5581 tactgttgca atggacacac ccgcccgttt tgcaacatcc ctaattccta ccataatagc
     5641 tcctcttttc atatttgaaa gtgtttacta acatataatc atacattttg aaaataagca
     5701 aatttcgtta aaaataggca ctgtaatcat aattaaaaac aagaaaaaat atccgaacaa
     5761 tttagagtaa ataatgctaa ttctagtgaa tttgcgacga ggaactgcta gatcacagtt
     5821 cattctctct atcgttcgga tattttattg ttaactatac ttatttcaca gagctttttt
     5881 atttgatttt gtgaacagag tctcgagaaa tcaattctcc ctctaatagt agtacttgat
     5941 tttttatttc ttcttgtttg gccagtccaa tcagttggtg aaaagcaatc gcccccttgt
     6001 tacctggatt tagtcgtatt gttgtcaacg caggatccat taattctgca aaagtaatat
     6061 catcaaatcc aataatcgat aattgttcca tcactcgttt attcttattt tttaaaaatt
     6121 ttactaattt agccgctaaa taatctccag taacaacaat tgcagtaaat tcagctaatt
     6181 cttgataaat tcgctcaaat tcagagttgt tatatatcca atgcgattta aaggttaaat
     6241 gatatttctt taatgtatcg atataagctt gatgtcgttc ttgaataaca cttggttcac
     6301 ctacaatgta gtcaaaagaa agaaacgcta ttttctcatg accataatga atcaaatgtt
     6361 ccactgccaa tgtactagct tggtaatcat ttgtatttac aaataaacga ttcatttgtt
     6421 gttcaagttc tcttaatgtc tcttttgaaa gatgcgtatc aatataaaca acaggaccat
     6481 caaaaacgtt atttaattcc aaaaattctt ttacacgtac accaacaaca atgaacccct
     6541 caaaacgcca atttctctga atagttttaa catcttctgc tgaattaaca ttatgcacca
     6601 atacaaaata accatgattc tttgcctgtt cctcaattcc agacaaaact tctgcggtaa
     6661 atggatcaga aaatacaaaa tcagcattat caatagccga aaaataaagc aatcctatta
     6721 aaaatgcaat cgtcttaccg attccctgac agacagaaca cattccacta ggatcattaa
     6781 atgaataagc atttgcatat cctaaactag gtgttgcaaa acatgaaaac aacacacgaa
     6841 ataacgcatt gatgttagta atcgtcccta acgttgagcg cgcactgcca ccaatcttct
     6901 tttgattgac cacaatcgcc gtagaaagat tatcaatctc atcaacctct ggacgactgt
     6961 atttaggtaa aaataaacgg gtaaaactat cataggtgtc attcagttga cgaccagctt
     7021 cttgggtaat agtatcaaaa acaatcgatg attttcctga accagatacg ctggtaaaaa
     7081 tactaatttt atttttggga attttgagac tgacattttt aagttgtttc tcttgcgtgg
     7141 ataatttcaa tatactcgtt catttggtac tcccctttat tttgaaatag ttgagaatgt
     7201 ttcaatgaga cacggtattt ttggcagatg tttagtttac ctagaaagag gtataaaaat
     7261 caagaaaaaa agagaggctg actcataagt agttttatta gtcgaaaatg atagtttata
     7321 aaaataggaa ccgcatcaaa tcaacattct ttagaatgga tttgatgcgt ttttctttat
     7381 tttttgactt atgagtcaac ctcttactct tcgtcatgga tttgattagc taattattgc
     7441 tccacttgtt cacgaatgaa atttaagtac tgcggtaaaa ataaaggaat ttcttcaaag
     7501 cgtggacttt gattttcata cgcttttgca tctaggtttc cttcccctgt agcaatttca
     7561 atcaataatt gatttgggtc acgataataa agtgatacaa aaaattcacg atcttttatt
     7621 cctgaattta taaaattgcg ctcttgtaat tgtttgtcga tgcgttgtaa atcagattta
     7681 ttttctacgc caaacgcaac atgatggacg ctaccaatcc cttcaatggc aactggattc
     7741 tcacgatctt gaataatctg aacttcttga tagaactgag tgtcttgatt ttctaagatt
     7801 gtcactcgtt gatgacccga aaagaaactg acgtattctt tgtgctacca gcctaccgac
     7861 aataactctc gttcagtcgc atcggaatat tgtacgcgta gttgaatggc atcaattcct
     7921 aaaatggcat gctctgtagg gatctggtct gtgacatacg ggaaataatc atcaatatct
     7981 tcaaattcgc gtaagggaac taaagctaat cgtgttgcat ctggcccttc aaaacgtaag
     8041 ataggtcgtc cagaaaatga ttcgattcca taatggcaaa cgccttgtgt ttcaaaatat
     8101 atttcccaaa aatgcaatga tgcttcggaa ggaactttta gtatcgttcg ttcaatcgta
     8161 ttggttccaa aaaactggtt gttcccttct tgtacttcaa aaaaagtcag ttcagttccc
     8221 acacggtggt gattatctga aaaaaacagg tgatacatgg tatgatcgtc ttgattaatt
     8281 gttttcatta gtaattttaa accgagtata ttatgataaa agtcaaatgt cggtcgaata
     8341 aagcgattca caatagaaat gtgatgaata cgtgcgggca taatatctaa aaatgagaat
     8401 caaacctata aaagatggtg aacaactttt gttttcatca gaagaaaata agccccttta
     8461 tttagactat gtaaatcaca atttgaaaat catcattaaa gagaataact taaaacatat
     8521 cacacttcat ggttttcgtc atacccattg tagtctgctc tttgaatctg gtgcgtctgt
     8581 caaagaagtg tatgttcgac ttggacatac agatattaaa accactatgg atgatatata
     8641 cacccatatc gcgaaaaagc agacagagga aacggcaatt cgttttgcag atttcatgag
     8701 ttctacagag gaaaagcaag gaagtttgca gaaggtcaaa agtatggtca aaaacgaaaa
     8761 aagcagacta tccctattag atagtctgct aaacgttgag ttatcagtat ttttcttatt
     8821 ccgcagcgtt tgcgtttttg agaccgtatt gattctcttt ttttaatttc atccttttcc
     8881 aacctaaaaa tgttatttaa ttacttagtt tatataacac gaacctatac caagtaccag
     8941 taaccccagc ttttattccg cgaactttat gaaataaaat cgttctagat aaaaatagct
     9001 tttaatcttt gtctttactg tttataaaaa tttttaaatt atacacctag tttaaatcca
     9061 cgaagtgatg ctcctatttc atcttcagtt ataccaatgt acctttttgt aattgcttgc
     9121 gagctatgat taaaaataaa cattaaggta gcaatgtctc tagttttctt gtagtagtga
     9181 tacccaaaag tttttcttaa tgtatgtgtt cctatatcat ctcgtcctaa tttttctgct
     9241 atatctgcat atatctgata gacacgatcc cttgttatat gtcgaccttg ttgccgactc
     9301 ggaaataacc aatcattttc ttctttacct tcagtatata ataaaatttc ttctctcaaa
     9361 ttatctataa aaatcggttt tcgcttacct gttttatgtt caacaataat aggcttactt
     9421 ttgcctcgta tatctccaac ttttaatcct ataatatctg acatacgtaa cccattattg
     9481 attccaaaca aaaataaaaa actatctctt tccggattac ttgttcttct tagccaaaac
     9541 tgaaaatctt gtatctcttc atttgtcctt aacggttgta cattatagct cactattgtt
     9601 tcccttctta aaaatattct gagttaatga ttatgtatat attttaccat aatcattaac
     9661 ttatatatat aaacttttga aagtcaataa aatcaaggga tttagtttat gattttatat
     9721 catttcagta actcagaata ttccga
//
LOCUS       DAXWLF010000102         9688 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXWLF010000102 DAXWLF010000000
VERSION     DAXWLF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.102"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..925
                     /gene="yidC"
                     /locus_tag="ACGE4U_001880"
     CDS             <1..925
                     /gene="yidC"
                     /locus_tag="ACGE4U_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFF8394013.1"
                     /translation="NWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVHPMGQA
                     ITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVEATQQK
                     LKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARYTEGIR
                     ESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAMIVFMS
                     ISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPRKDVTP
                     AEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(1106..1510)
                     /locus_tag="ACGE4U_001881"
     CDS             complement(1106..1510)
                     /locus_tag="ACGE4U_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8394014.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(1526..2335)
                     /locus_tag="ACGE4U_001882"
     CDS             complement(1526..2335)
                     /locus_tag="ACGE4U_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8394015.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(2322..3212)
                     /locus_tag="ACGE4U_001883"
     CDS             complement(2322..3212)
                     /locus_tag="ACGE4U_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8394016.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(3225..3704)
                     /locus_tag="ACGE4U_001884"
     CDS             complement(3225..3704)
                     /locus_tag="ACGE4U_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFF8394017.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(3721..5487)
                     /locus_tag="ACGE4U_001885"
     CDS             complement(3721..5487)
                     /locus_tag="ACGE4U_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFF8394018.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFATGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(5533..6474)
                     /locus_tag="ACGE4U_001886"
     CDS             complement(5533..6474)
                     /locus_tag="ACGE4U_001886"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFF8394019.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(6471..7466)
                     /gene="lacD"
                     /locus_tag="ACGE4U_001887"
     CDS             complement(6471..7466)
                     /gene="lacD"
                     /locus_tag="ACGE4U_001887"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8394020.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(7459..8652)
                     /locus_tag="ACGE4U_001888"
     CDS             complement(7459..8652)
                     /locus_tag="ACGE4U_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8394021.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(8670..9398)
                     /locus_tag="ACGE4U_001889"
     CDS             complement(8670..9398)
                     /locus_tag="ACGE4U_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8394022.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         2731 a         2169 c         1681 g         3107 t
ORIGIN      
        1 aaattggtta ttgggctctg ggcttgtcac gatcatgctt ttcttgtccg gttgtgtccg
       61 catggattca aatggtaacc ctgacacttc aggaatcatt taccgtatat tggttcaccc
      121 aatgggacaa gcgattacat atttagtgga aaactttaat tggtcctacg gctgggctgt
      181 tatcttgatg acagtcatcg ttcgtatcat cattttgcca ttaggtatta gtcaatcgaa
      241 aaaaacaatg atccaatctg aaaagatgca agcattgaaa ccacaagttg aagctacgca
      301 acaaaaattg aaagctgcga caactcgaga agaacaaatg gctgcacaag ctgaaatgca
      361 acaagtttat cgtgaaaatg gcttaagtat gactggtggt atcggttgtt taccgcttct
      421 gatccaaatg ccgatctttt ctgcgctata tttcactgct cgctatacag aaggcatccg
      481 tgaatcaacc ttttacggga tcgacctagg taaccctagc ttagttttag tagcgattgc
      541 cggtattgct tatttacttc aaggctatat ctcaactatc ggtatccctg aagaacaaaa
      601 gaaaacgatg cgtacgatgt tgatcgtatc acccgctatg atcgtcttca tgtcgatcag
      661 tgccccagct ggtgtcactc tttactgggt agtcggtggg atctttagtt gtctacaaac
      721 attcattacg aatgtcatca tgaaaccaag attaaaagcg caagttgcgg aagaattaaa
      781 acaaaatccg ccaaaacaag tggtgacgcc tcgtaaagat gtcacacctg ctgaacctgc
      841 gaagcaaaca aaaaaacaag tacaagcacc aaaaaacaat gcgaagaatg gtcggaatgc
      901 tggtaaacag caaaagcgta aatagttatt tgtaactagc ttcttcgatc gacccagatc
      961 atttctgatt ttatgacgat aaaagccaac gtactgtgaa atatagaaaa ctatatttcg
     1021 cggtacgttg gcttttttat acacggcctc tttatacatc cgtatttata tccagttgta
     1081 tcaccagtca agaagttgtg cttgattatt cttcttcatc gtctcccatg ccagcaatca
     1141 cttggttcac tgaaacgata ctctcacgac ccgaaccaat aaaatctggt tctgctccaa
     1201 tcatttgcga attgatgaat tcgatgacca tcggcaaatt catgccagca tatagttcga
     1261 tcgtttcacc ttccatgatt agcttggaaa cggtatttgc aggtgtccca cccaacagat
     1321 cgcaaagcac tacgtaatct ttcaatggtg ctgtaacgga gaggaatctt tctttatacg
     1381 tgtccgcgcc atcttcaggc aataaaggga tcgtatagat attttcttgt ggtcccatga
     1441 tcatttctgt actgtttttt agttcttcac aaaatttacc atgactaatc aagactagtt
     1501 gtctactcat ctttccaatt ctcccttatt tcgaaatcac acctaatgca gataatgcta
     1561 cacatacgat caatacgatg aatattgctc gagtaggtgt catatttttc ttacctaaca
     1621 tccaataaac gccaccaacg attccagctg gtaaaagttt aggtaagatc atatcgatat
     1681 agttttgcat attcaacgtt acatccccaa tcgttggtgc ccatgagaat ttaacattga
     1741 tcatggttgc tactaacgct ccaaccatga aaactcctag taaagtagcc gcatctgtca
     1801 atgcagtcaa gcgtgattgc atcgtggtaa ctagagaaat cccttctttg taggcaaatt
     1861 ctagttgttt ccaacgaaag accatgacag cgacctgagc aaggatccag ataaggatac
     1921 ctactggatt accatctgtt gccatatttg cggctaaagc tccaaatatg gtcgggatca
     1981 atgccgcaaa tacagaatca ccaattgcag cgaaggaacc catcaatcct gctttcatcc
     2041 cagaaacagt ttcctttgaa gagactcctt ctttttcttc gatagcaaga tcgatcccag
     2101 taatgatcgt gttaaagaaa ttagacgtat taaaaaattg cgtatgtgtt ttcatcattt
     2161 cttttaattc tggtgtatca tcaccataga tttttctaag ttgtggcaag atagtataca
     2221 gataacctga accttgcatt ctttcatagt tccaacctaa ttggaaagta aacaaacttc
     2281 tacgattgat ctgcttaaaa tcttctttgg tcaatttata attagatttc gtcatcttcg
     2341 atttccccct catcagattg tgattgctga cttactactg gatgttggtc attcttttga
     2401 tcattcttat aactgatcgt tgccagtgct agaccaatca atgccacagc aagcatcggt
     2461 aatgaattga atgttcctgg gaaatctttg accacgctag ccacacttgt tcctaacaat
     2521 tggacattcg agaacactgt tgctaataac gctgtgatcg taaatcctaa aatcagataa
     2581 gaaatgtgtt tctttactgg taggtagcgt agtaaaattg caaacccaac tgctggtaag
     2641 actgcccctg caacagataa gccatctcct agccatttta ggtcgccgtt taaaacagcc
     2701 acaacatttt ggactaagcc accaccgaat gctagggcca agaatacagg aaccattctg
     2761 gaaagtgccc aaggtaatgc ccccatcaag aagttccgtt caattgcttt gtaattcatt
     2821 gtttccacat gttgatcgat ccgatgtgca aaatacgtat tcgcaaaacg tgccaaaata
     2881 tctaattgga tcatcaaact tgctacagga accgcgatgg ccgcaatcgc ttgttctggg
     2941 ttcatcccta gagaaatgga gaaagctgtt gctaagatcg ttccagagtt ggcatcgatt
     3001 tttgatgcac caccaaatgt ccctacccct aaaacagtca actgcatact tccaccaatg
     3061 attaaacctg cttttaaatc acccattact aacccagcat ataatcctgc aaaaactggg
     3121 gcgctcattg aagaataaat ctgtaattca tctaaaattt gataccctgc atataatgtt
     3181 aataataata tctgccacca ttgaatatcc atgaatcatt cctcctattt gattaaaaga
     3241 ctcataaagt cttctgcttt gtcatttggc accatttgag cgatcaaatg aacgccaagc
     3301 tgatctaatt tcttgaagac ctctacatcc gcatctacga cattgattga ttttgtgatg
     3361 gaacgtgttt catctgtttg tgacatattg ccgacattta attctttgat cggtacacct
     3421 ttttctacta gcgctaagat acgatctggt tttttcgcta cgatcagcac tcgttgagaa
     3481 tcatatttac ctgctaaaat atttgcggct gctttttcaa taggtaaaat acttaatttg
     3541 actccagctg gcgttgccaa ctttaaccca cttttttcga tcgcatttgt tgctacctca
     3601 tcgtctacga ccatgatccg accaatattc aactttgtcg tccaaagatt tgccacttgt
     3661 ccatgtatca aacgtccatc aattcttact ccaataattg ccatcatcca ttctcctttt
     3721 ctaacttttt tctattggtt ctttgacaaa atctataact tcactgaatc taccttctgt
     3781 ttcaaagata acgatttcat tttgtccttt tctaaataaa ccctcgggaa caaacagtga
     3841 aagaattggt cctacttccc aaaaacgacc aatattcgtg ccattcaccc aaacgatccc
     3901 tttcccaaac tcactaaggt ccaaataagt atcaccgatt tctgacagtt ccgcttcaaa
     3961 gcggtaaaat gctggttgac ctgcttccca tttttttgaa aaatcgatgt gttctgtagt
     4021 ctctaaaggt aaacaatact gtgtccattc tgtgataaaa tgaagatcgc tcatcactcc
     4081 tgtacgtatt ccttttcttt gcgtatcagc tagcagcttg tgtccatagt tgacccgtcc
     4141 catattttca aataatagat caatttgatg gtcttctttc tcaagtgtca atggaatggt
     4201 ctcaccgatc tctgtttggt attgaatcgc ttgcttttct tcatcgagaa aaacatggac
     4261 ccgatcacgt ccgtcgatga tccgtaattt ttcattgacc gcatcttttt tgatcgttgt
     4321 tcgatacaat aggtacccag tattctgacc caattcttcc attgtttgag gataggtcga
     4381 gtggatcggc ttgctgatgg tatccagtgt tgcgaaaaag ctgacttttt ctttcaagtt
     4441 gattcctttt ttgacgattg tttcttttat ccatggttcc atttgctgca tttctggaaa
     4501 attttctgcc atcattcgtt tcagagcaaa atatttttcc gttggatttc cctgctcatc
     4561 caatggtgca tcgtaatcat aggaagtgac ttgcggcaaa tcaatcgcgc ctctggctga
     4621 acagccattc ataaacccaa aattggttcc gccatggaac atatagagat tgatactgcc
     4681 gatctgtaat gcttctttga ctgcatccgc taattcttcc ggatctcttt taatgatcgg
     4741 ctctttccaa cgattaaacc agccatccca aaattccata cacatcaacg gccatttttt
     4801 accatgtgct tcaaaaaact gggccatatc tgcaaaattt tcttttgctt ttgagccaaa
     4861 attccctgtc gcaaaaacat catcgtcaat caaaatgcct gctcttaatg tggcttgcca
     4921 aggtccatca gaagtgaata gcggtacatc gattccacct tcctccaagt atttttttac
     4981 ttggcgcagg tatgctttct cttcaccata cgaaccgtac tcattttcta cctgcaccat
     5041 taagacgttg ccaccatgag tgactagatg aggaacgatt ttaggtagta gcgtttggta
     5101 ataccgtttg acatacttca tgaagcgagg atccgctgaa cgaatacggc actcctttgt
     5161 caatagccaa gcaggcaagc caccaaattc ccattctgca caaatataag gggctggacg
     5221 tacgattgcg tataatccaa gggattgcgc taactctaga aaatgggaaa gatccaatat
     5281 tttcgaaaaa tcataggttc cttcttcttt ttcatggaga ttccaaggca cataggtttc
     5341 cacagtatta aatccaagcg ctttcaaatt gtgcaatgac cgctcccaat tcgtcggatg
     5401 cactcggaaa taatggatgg caccagataa aattttgaaa ggttgaccgt ctaacaaaaa
     5461 ctcttctttt atttcaaatc tagtcaagca aatttcctcc tcgtttttag aaaccgcttt
     5521 ctaattttat aatcaacgaa tctttctgac gttgatttcc cccctaaaat aatcgacttt
     5581 cacaggatcg atccatcctg ttttttcttg ttgtgcattc gccatcccac aggccatcgc
     5641 aaacgcaagt gtttcttcaa tcgtatagct ctttgataga gcatgtgcta agcctgcaat
     5701 agtcgaatct cctgacccca caggattcac caccgcgatt ttaggaatcg taacctgata
     5761 gaagtgtctc cctactttag cgattgctcc ttttgcgcct agcgaaagaa cgatccaatt
     5821 gattccttca aaaagcggat gatccaataa tgattggatt tcatctaacg aagtagccga
     5881 agaaattgtt tggtggacca actctcctaa ttcttccaaa ttaggtttga tcaaaaatgg
     5941 tttttccttg ccttccaaga cttcaagcaa ggtttgacca gaagtatcta acaatactcg
     6001 tgttcctttt ttattcgtta gggcaagtaa ctctgcataa taacttttag aaaatccttg
     6061 aggcagacta cccgaaatcg taaagatatc agttgtttcc attagtcgtg caaaatgctc
     6121 ggtaaattta tgttgctctt cttccgtcac aacaggaccg ctttccaata gttctgtttg
     6181 attcactcca tgtaaaatag caatcgaatc tctcgtttcc cctttgatag gtaaaaattg
     6241 atgcgttatc cctgattgat cgagttgttc tttgatatat gctccatgct gcccgccgac
     6301 taatccagta gctaaaacat catctgctaa taaatgaagg acacgagcaa cattcaagcc
     6361 ttttcctcca gccgttttgt tgaccgtttt gacccgattg acttggtcga tccgtaattc
     6421 atccaacgtg taagcgatgt caattgaagg attcatggta actgtcgtga tcattttgct
     6481 gtcaccgaat cttcttttac tttgtcaaac caaggggtgg cgttttcatt tatcacttta
     6541 ttcaacgatt cgatattttg tcgcccttgt gttctcaacc actcaatcgc accttctcta
     6601 ccattttcaa catatgcttt gactccatct ttccaagtcg cacgtccgca taacacaccg
     6661 ttgaatgttg aaccagattc atgagcaaag atcaatgttt gttggaacaa tgcagtcgaa
     6721 acccctgcac tcaagaagat aaatggtaac ggtgttgcat cactttgttc cttgaagaat
     6781 gccgctgctt cttttttact atacgcaatt tcaccatcag aataaccttc tacatagttc
     6841 atattgactg gcacttctac tttcaataca tccactttat atttaggatc agaaaattct
     6901 ttcatcatct cgatcacttt atgcggtttg atcttcgcat attctaatga agtgctgtca
     6961 ttggttttgg catcatacga aaccaattct agataaaacg gacgcttttc acctgcacat
     7021 tcactgccta aacgttcgat aaatacatgt ttcaaatgat tgatctctgg gtcttcatct
     7081 acatcataat aaagcaaaaa cttgatcgca tcggctgttt gttcctttaa tctaagcgta
     7141 gaccaatccg ctaataaatc aggcagacgt cctggagttg tcgcatcata acccgttttt
     7201 tcataggcta aaagtagtcc tgcttcctga tgtctggctt tagcagctgg caatccatac
     7261 tctggatcta gtaaaatagc agaagcataa ggcgttaatt ctgctgatac taattctttg
     7321 aattcagtga tctgttgcgc cgttggttct tttcctaacg ctttgatcat ctttttcaaa
     7381 gcaccgcgtt gatcaattgc caatgcactg atgattcctt cttttgttga taaactcttc
     7441 atagctgcaa ttttttcttc attcatcctt cgtttcccct ttgttcacat attcatggat
     7501 aatgacacct ttgaccactc gattgactgt acctgtaggc gatggtgtat ctggtttatt
     7561 cgctactttt atcgacgtat cgagcgcaat gatttgggct gtcatgatca taggtaaggc
     7621 aagatacgca tcaggtaggc tgatcgaagc ttctaacaaa agacttgtac ctgtgaaatc
     7681 gatgtttcct ggttgggcga tcgcgatgac actcgtggta atttggtcat cttttatttc
     7741 ttctagaata tctaagtcat aatttcttgt atacggttgg ttattaacaa aaccaataac
     7801 aagtgtttcg ctatttaaaa acgattttgg tccatggcgg aagcccatag aagaatcaaa
     7861 gatcgttgcc actttaccgg ctgtcaattc caatattttc aattgtgctt ctctcgttgc
     7921 accagccaaa cttcctgaac caagataggc gatacgttca aatgacttag gcaataattt
     7981 ttggatttct tgatctcgtg aaattagatc ttctccagtt ctgatcaatt gttgaagata
     8041 actttctttt ttctcaacgg cagtttggtc gaatacccat aacgcactca acaacatgca
     8101 cgtaaaactt cctgtcatgg caaatcctgc atcatttgat ttttctggca tcagtaaaag
     8161 ataatttcgt tcatctgtcg ctgcattctt tgctaactga ccttctttag cacaagtaat
     8221 cgtcaaatga aacaatgtct tgatttgttc attggcaatc ttgactgttg ccacactttc
     8281 tggactattt ccactacgtg caaatgacac cagtaatgtc ggttcatccg gaaatagaaa
     8341 ttcttttggt gaagacacga tatctgtcgt accaaagcta tgaaaaataa agtgatcgtt
     8401 atcaccgtat tcatttaaat aaggtactgc tgtatccccg acatattgtg atgtgcctgc
     8461 accagtaaaa atcactcgaa ctcttttgcc atttgctgct ttttttatct ggtcaaaaaa
     8521 caatgctaat ttttcttgat tctgtgctaa tatctcagct gtttctttcc ataattgcgg
     8581 ttgctgtttg atttctcgcg tagttatttc cgcacccaac tcttttaatt tatccttcga
     8641 tagttcaaac atgttgtttt ccctctcaat cattttcgga cgtggcggat cttataatga
     8701 aactggtctg ctctcgctac actatccgta tattcaataa tttcattttt cacattatag
     8761 gtcgttcggc ttagatataa taccggggca ccatcagcga tacgaagcgg atcagcatct
     8821 ttcttttgaa cgatacttgc ataaaattct tcatctgcta attggacttg ctgttcataa
     8881 tcctgagaaa acacatcata caatggctta ctttcaagta atggcagatc aagaccaata
     8941 aatagttttg caggtaaata agaacgttcc accatcaatg gttcgtcatc cgctaaacga
     9001 agtcttttca atttgaaaac tgtttcgcct aaattgatgt ttagattctt ggcaaccttt
     9061 ttgtttgctt ccatttgctt gaattccaag atgactgtat taggcacccg tcccaattcc
     9121 ctcatctctt ctgtaaaact atacgctccc gccagatcag acgacttatc tgatagatca
     9181 gacacatatg ttccttttcc gtgtcggcga taaatatacc cttgcgtctc taattcttgc
     9241 aaagcaaggc gaaccgttgt ccgactcact ccataatgct gacaaagttc tctttcagat
     9301 aacaatttgt catgaggtgt catctgtgtt tcgatacgtt ctttcaatac gtcaactagt
     9361 tgataatata atgattttcc tttacttgag ctctccataa aaatacctct tctttactgg
     9421 ttataaccac aacacaagaa tacccacata ttcctcattt gtcaaagggt ttttggcgta
     9481 aaaaagctat tataacaagg tttcaaggta atttagaact ggtatcaaaa gatacctcaa
     9541 aaataccagt tgacaaataa aaaacaaaaa aaagctgaat cttggcaaag attcagctaa
     9601 atggtctaga gaagtaaaga aaaaatattc tttatacatc tgtggctatt tctactatcg
     9661 acaagcaaat gggcctaata gactacta
//
LOCUS       DAXWLF010000103         9453 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXWLF010000103 DAXWLF010000000
VERSION     DAXWLF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.103"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            148..3723
                     /locus_tag="ACGE4U_001890"
     CDS             148..3723
                     /locus_tag="ACGE4U_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8394023.1"
                     /translation="MISNKKRWLKVALLVAFCQIAFLASMTTQAEEIVQETTVSQVSP
                     TSNEVEVQSTLPKEAVKEETPSEVVPETEAPSTNEKTVLDETIGKSQQPGDSPVVAEE
                     TPVEAHEAQAIPEKDKSLEESGSTEINEGTEPVQLEDSKEVTTPIESTKEEMDSNTSL
                     SSEKEEKTVLLTEPREPELKKVEEFVDTKKEAVSPTSSKEDTLIVQQTARSARVRRAT
                     NTAVLGGRKIDWGENIGVISSANNPNFTLNYMVRFEINGQVAFCIEPGVAAAEGQVYT
                     ATALENYLKDLNIRKKTSLISYLGYVTSKDKSDEQYIAAQFMIWEVLGTKIPIKNIRI
                     DYDRRKAEIQRLLNNFDVRATFHNQTHTVKIGETLKVKSGNDFLQRVKDIQKPKGVNV
                     SIHDNEVWITITKESPEKAKIHFNQIEALGVPLAYRRPGAQTIGVLNPFVPGDSVVNL
                     TILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQANGKKIKEITLKNINGKVQAEI
                     KDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNTIAKDQVIKGTIDLVKVANKD
                     LVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTVVFDTYILSETK
                     GKEGYKLFEPFEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKVIALAGTQFKIW
                     DKYANNGKGGYVSMALPNDTEVSDIFTTNEKGYFVTTDTLKYGKNRYELREIKAPEKY
                     VLNKTPYVFSVTDDTNTIQLIYFANRLAKANVEVFKYDTSTNFDQKTAIAGVEFDLFK
                     KDETGTTFVGTFKTDKKGKINVKDLLVGDYFFKEVKPLAGYLALDKDVPFTVTVDKDG
                     TVIALEVENKRIPPKIGTTAIGKYTNQQKVTPLKKVTITDTVKYSNLIIGKTYTVNGV
                     LMDKEESITAGKDVPLLVNGKQITASKVFVAKNPNGMISLDFVFDASALAGKEIVVFE
                     NVYRDGRLVASHTDIHDKGQTVRIVNPMIHTKATGQNGEKQLQALPNQKVKELVQLND
                     LIVGQTYKVLVQGYITPDGVKLDGTSAEKIFKADKTTLEFLFEFLVDGRKLAGKGLSF
                     AEWLEAQSEPKDENDYEVRFEQIANHNVDLKSEDQTVYFVEVPKVPEPKIPEKSQPFK
                     EVKQASVVPMLPKTGSEDTVLWMIIGVLFVLAAGLTFYELRKVKD"
     gene            3823..4761
                     /locus_tag="ACGE4U_001891"
     CDS             3823..4761
                     /locus_tag="ACGE4U_001891"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8394024.1"
                     /translation="MKKKERKKRMKKLFFACLFSSVVLAGGINVFADEIGITPVTPPV
                     IEETTPVVPIPDISKIDESVPDQPTPVDPDISSEVTEPTTPVVPDITENSENETPVVS
                     PSTGETTNDKDTTPTTSESYSGTSSVDNDSKIDDFSSSSTTIDTPKETEKKNDLGAPK
                     NLTPENHIKTVSEKEMTVSVSSDGKIVQGTGTEKSIPIVTSNLEEINHVPTSETPLQV
                     ATGQAIVGVQDGVPLVQDNQGNLVEDLSIPVKKLPSGNIEIKTIDGKTKVLPKTGEEL
                     QISLTILGSLLTAVTGGIFYGKKQWRHLRHMFKKTI"
     gene            4780..5130
                     /locus_tag="ACGE4U_001892"
     CDS             4780..5130
                     /locus_tag="ACGE4U_001892"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394025.1"
                     /translation="MGLSYVAISQEEVLGKCFNQKHVGSTGERDRRGNMKKVEWEIFQ
                     MNSEKMGEIEVRVAVGGAPKAMRYNAEVLLANPRISRRADVRNIGNDGETNKIARNRF
                     YLLVDRVEVKGGNN"
     gene            5130..5561
                     /locus_tag="ACGE4U_001893"
     CDS             5130..5561
                     /locus_tag="ACGE4U_001893"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HFF8394026.1"
                     /translation="MTLEFDQGIVPGLVKETLGEIYFVKAQRPRMLYEDNENTGEIKE
                     RPVEFSSATQETMFVVNFPPKTNLDGLSFGDVVELEEVEFRFWISIDRESMSNRAEND
                     LKVSALRYKKTGKNVFQTSSSTRATYVKEKQDSNKESAKKE"
     gene            5829..7148
                     /locus_tag="ACGE4U_001894"
     CDS             5829..7148
                     /locus_tag="ACGE4U_001894"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8394027.1"
                     /translation="MFQYKGIRIRNRHKYASERFIFYLMLPVLLLMSGFFSLFYTTIT
                     WEWCCIYWIIAFGLAGLFAYMCLYFYFYNVYQRLVHKGKLAKMIHSRRLILERVLKNG
                     RRKVVYYPKIFYHYKNGLIHVRLPLDLGPFQDRFNEMAGQLEEAFFCDLVEEQREKNY
                     ICYTFLYDVEKNRIGIRDITVKDGIITLMKNVQWAFNKAPHAIIIGSTGGGKTYFIMS
                     LIYALLPYGTFDICDPKRADLASLEKIPIFKDQVFYGGGILSCLINLAKETANRYDFL
                     REQPEYELGYDYTYYHLTPHFLVIDEWAAYYASLDTKEKKKAMGCLNQIALMGRQCGV
                     FLILGTQRPDTKYLDGAIRDNFGLRVTLGRVSKAGYKMIFEHTVKSLFNKPIKGRGYI
                     DSGTDQVREFYAPFIDITRFNFFAEFKQLSDQLMAEKIKETKEGKNV"
     gene            7141..7305
                     /locus_tag="ACGE4U_001895"
     CDS             7141..7305
                     /locus_tag="ACGE4U_001895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394028.1"
                     /translation="MYEEIPATCGTRLEIKDLMIDSFLAIVFSEKRLSFFINKKVENR
                     WRLERMYPTS"
     gene            7844..9019
                     /locus_tag="ACGE4U_001896"
     CDS             7844..9019
                     /locus_tag="ACGE4U_001896"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFF8394029.1"
                     /translation="MDGTQLKQMRLSRNLTQKLVADTLGWPQSFLSMIENDQRSCSKE
                     QEEALCEFYANFSSFTSLEIKFDYVRVRVPTNDTKTVVEEILGMDFNSFFYAETRLYG
                     YIEMYQKGMIRVLNSKKGDDKGVLIELSGQGCRNYESVLDERNDSWYQFFTRCVSFNG
                     VPRRIDIAVDDFEELFSLHEFAQKLNRGEFDSKFRTWRFQEEKQLLDNLSSGLSVYLG
                     SSQSLMYFCFYQKNYEIAKKQKLPLEEVEVKNRYEIRLRDAKAVSFIEQYLNKVDFSD
                     LTMGILANQLIMFEGEKNANVWKPWGKLIQNVGKVNLTITPEKPNYNRKLMYLKTQPA
                     RTMKIVKTVDEILGLNRFGEILEETELREQDEKIVEDEVRAVYEYIQENPDAIHYLL"
BASE COUNT         3401 a         1374 c         1938 g         2740 t
ORIGIN      
        1 aaaagtttta tagacaaaga tgttcatagc gaacatcttt tttatttatt ttgagaagaa
       61 acagagcatg aaatgatgtc ttatttccat ccatatagtc tatttcaaac agaagagaac
      121 aattaaaaag aaaggagatt tcttgttatg ataagtaaca aaaaacgttg gttaaaagtt
      181 gctttactgg tagctttttg tcaaattgct ttcttggctt caatgacaac acaagcggaa
      241 gaaatagttc aagaaacaac tgtttctcaa gtttctccta cttcaaatga agtagaagtg
      301 caatctactt taccaaaaga ggccgttaaa gaagagacac catcagaagt tgttccagag
      361 accgaagcac cttcaacgaa cgaaaaaact gtactcgatg aaactatcgg gaaaagccaa
      421 caaccaggag atagtccagt tgtggcagaa gaaaccccag tagaagcaca tgaagctcaa
      481 gcaattcctg aaaaggacaa atcattagaa gaaagtggtt ctactgaaat aaacgaagga
      541 acagaaccgg ttcaattaga agattcaaag gaggtaacga ctccgattga atcaacgaaa
      601 gaagaaatgg attctaatac ttctctttct tctgagaaag aagagaaaac agttcttttg
      661 acagaacctc gtgaacctga attgaaaaaa gtcgaggaat ttgttgatac aaaaaaagaa
      721 gctgtttctc ctacctcttc aaaagaagat acattgattg tacaacaaac cgctcgaagt
      781 gcacgggtgc gacgtgcaac caatacagca gttttgggtg gtcggaaaat tgattggggc
      841 gaaaatattg gtgttatttc tagtgccaat aatcccaatt tcactttaaa ttacatggtt
      901 cgttttgaga ttaatggaca agtagcattt tgtattgaac ctggtgtcgc agccgcagaa
      961 ggtcaagttt atacagctac tgctttagaa aactatttga aagatttaaa tatacgaaaa
     1021 aaaacaagct tgatctctta tttaggatat gtcacaagca aagacaaatc cgatgaacaa
     1081 tacattgcgg cacaatttat gatttgggaa gttttgggca caaaaattcc aattaaaaat
     1141 atccgtattg actatgatcg tcgaaaagca gaaattcaac gtttgcttaa taattttgat
     1201 gtaagagcta cttttcataa tcaaacacat acggtaaaaa ttggcgaaac gttaaaagta
     1261 aaaagtggca atgactttct acaacgtgta aaagacattc aaaagccaaa aggtgttaat
     1321 gtatctattc atgataatga agtatggatt acaatcacaa aagagtcacc agaaaaggca
     1381 aaaattcatt tcaatcaaat tgaagcatta ggcgtgccat tagcgtatcg tcgtccaggt
     1441 gctcaaacaa ttggggtgct gaatcctttt gtacctggtg actctgttgt caatttgacc
     1501 attttgaaac aaggaaacat tcgtgtcttt aaagaagatg cagaaaccgg tgtgaaagca
     1561 caaggtgcag cttctttaga aaatgcagtg tatggcttgt atcaagcaaa tgggaagaaa
     1621 atcaaagaaa ttacattgaa aaatatcaat ggcaaagtac aggcagaaat caaagatctt
     1681 gatcctggta aatatgtttt aaaagagatc aaagcaccaa aaggttatgt actttctgat
     1741 gaagcaattg aaatcgtcgt tgaacctgga aaaactgtca atacgatcgc aaaagatcaa
     1801 gtgatcaaag gaaccattga tttagtcaaa gtcgcaaata aagatttagt cgattcaacc
     1861 aatccagaca acaaacccaa attagctggt attgaaatct ctttgacgtc aaaaacaact
     1921 ggaaaagtgg tgaaaacagt tgtgacagat aatgatggtt acgctacttt tggtaaaaat
     1981 accgttgtct ttgatacgta cattctttct gaaaccaaag gaaaagaagg atataaacta
     2041 tttgaaccat tcgaagtcac aatttctgaa aatggacaaa ccttccatta tgttttagaa
     2101 gacaaagtaa tcgaacaacg tcttaaagtg gtgaaagtgg atcaagaaac agggaaagtc
     2161 attgctctag ctggtaccca attcaaaatt tgggataaat acgcaaacaa tggtaaaggt
     2221 ggctatgtat cgatggcatt accgaatgat acagaggttt cagacatatt tacaacaaac
     2281 gagaaagggt actttgtcac aacggataca ttgaaatacg gaaaaaatcg ttacgagttg
     2341 cgagaaatca aagcacctga aaaatatgtt ctaaataaaa cgccttatgt cttttcagta
     2401 acagacgaca ccaatacgat tcaattaatc tacttcgcta accgtttagc gaaagccaac
     2461 gtagaggttt tcaaatatga tactagtacg aattttgatc aaaaaacggc gattgctggt
     2521 gttgaatttg acctattcaa aaaagatgaa acaggcacaa cctttgttgg tacattcaaa
     2581 acagacaaaa aaggtaaaat caatgtgaaa gacttattgg ttggcgatta cttctttaaa
     2641 gaagtgaaac cattagcagg gtatctggcc ttagataaag acgtaccatt tactgtaacg
     2701 gttgataaag acggtacagt gattgcacta gaagtggaaa acaaacggat tccacctaaa
     2761 attggtacta cagctatcgg taagtataca aaccaacaaa aagtgacccc attgaaaaaa
     2821 gtaacgatta cagacactgt aaaatacagt aatctaatca tcggaaaaac ttataccgtc
     2881 aatggtgttt tgatggataa ggaagaatct atcacagcag gaaaagacgt accgttgtta
     2941 gtaaatggta aacaaattac cgcaagtaaa gtgtttgtcg caaaaaatcc taacggaatg
     3001 atttcccttg actttgtctt tgatgctagt gcattagctg gaaaagagat agtcgtattt
     3061 gaaaatgtct atcgtgacgg acgcttagtt gcgtctcata cagatattca cgataaagga
     3121 caaacggttc gtattgtgaa ccctatgatt catacaaaag caacaggtca aaacggcgaa
     3181 aaacaattac aagctttacc aaatcaaaaa gtgaaagaat tggtacagtt gaacgactta
     3241 attgttggtc aaacgtacaa agtgctcgta caaggttata ttacacctga tggtgtgaaa
     3301 ttagacggta caagtgctga aaaaattttc aaagcagata agacaacttt agagttctta
     3361 tttgaatttt tggtagatgg acgtaagcta gcaggcaaag gattatcttt tgcagaatgg
     3421 ttagaagccc aaagtgaacc aaaagatgaa aacgattatg aagttcgatt tgaacaaatc
     3481 gcaaaccaca acgtagactt aaaatcagaa gatcaaacgg tttattttgt agaagttcca
     3541 aaagtaccgg aaccaaaaat tcctgaaaaa tcacaacctt ttaaagaggt gaaacaagct
     3601 tcagtagtac caatgttgcc aaaaacagga tcagaagata ctgtattatg gatgatcatt
     3661 ggtgtgctat ttgttttagc agcaggtctt acattttacg agttacgcaa agtcaaagac
     3721 taagtaggac aaacaagcgg ttagatgcct aatcgcttgt ttggttttaa aaaagttatt
     3781 gcataagtaa cctttgaaaa gataagaaat gatctttgtt ttatgaagaa aaaagaaagg
     3841 aaaaagagaa tgaagaagct atttttcgca tgtttattta gttccgtagt tcttgctggt
     3901 ggaataaacg tttttgcaga tgaaataggt attacaccag ttacgccacc tgtgatagaa
     3961 gaaacaacac ctgttgttcc aattccagat atttctaaaa tagatgagtc tgttccggat
     4021 caaccaactc ctgttgatcc ggatatatct tcagaagtaa ctgaaccaac aacacctgtt
     4081 gttcctgata taacggaaaa ttcagaaaat gaaacaccag ttgtatcgcc ttctactgga
     4141 gaaacaacga atgataaaga tactacacca actaccagtg agtcttattc gggaactagt
     4201 tcggtagata atgattcaaa aatagacgat tttagttctt catctactac aatagataca
     4261 ccaaaagaaa cagaaaagaa aaatgattta ggagctccca aaaatttaac acctgaaaat
     4321 catataaaga ctgtatcgga aaaggaaatg actgtttcag tcagttcaga tggaaaaatt
     4381 gtacaaggaa cgggtaccga aaaatcaatt cctattgtga caagtaattt agaagaaatt
     4441 aatcatgtcc caacatctga aactccttta caagtagcga ctggtcaagc gattgttggg
     4501 gtacaagatg gtgtaccact tgtacaagat aatcaaggga atttggtaga agatttatcc
     4561 attcctgtaa aaaaactacc tagcggcaat attgaaatca aaacaatcga tgggaaaaca
     4621 aaagtattgc caaaaacagg tgaagaactt caaattagtt taactatcct tggttctctt
     4681 ttaacagcag taaccggggg cattttttat ggtaaaaaac aatggcgtca tttgcgtcat
     4741 atgtttaaga aaactattta gtaaggagag agaataaaaa tgggtttatc ttatgttgca
     4801 attagtcagg aagaagtatt gggaaagtgt tttaatcaaa aacacgttgg ctcgacaggt
     4861 gaacgagatc gtcgaggaaa tatgaagaaa gtagagtggg agatttttca gatgaactct
     4921 gaaaagatgg gagaaattga agtgcgtgta gctgtcggtg gtgcgcctaa agccatgcga
     4981 tacaatgcag aagtgttact ggcaaatcct agaatttctc gtcgagcaga tgtacgaaat
     5041 attggaaatg acggtgaaac aaacaaaata gcacgtaata ggttttattt gttagtcgat
     5101 cgtgtagaag taaaaggagg aaataactaa tgacactaga atttgatcaa ggaattgtcc
     5161 cagggttggt aaaagaaaca ctaggagaaa tctattttgt caaggcacaa cgtccacgta
     5221 tgctgtatga agataatgaa aacacaggag aaattaaaga gcgtccagtt gaattttcaa
     5281 gtgctaccca agaaacaatg ttcgtggtga attttccacc aaaaacaaat ttggatggat
     5341 taagttttgg cgatgtggtg gaattagaag aggttgaatt tcgattttgg atttctattg
     5401 atcgcgaatc aatgagtaac cgagcagaga atgatctgaa agtatctgcg cttcgttata
     5461 aaaaaacggg aaaaaacgtt tttcaaacgt cttcctctac tcgtgcaaca tatgtaaaag
     5521 aaaaacaaga ctcaaataaa gaaagtgcaa agaaagagta atggtttcca ttgctctttc
     5581 ttattttaaa gctagaagaa gtagctgatg tggagaaaat tgaattggct ggctatatta
     5641 ttgggataaa tttttgaatt tttaagaatt tagatagtgg ttagatacga ttgattcttt
     5701 gtgtattttg tattatttaa gtattgtgtg gtatttacat taagttaata ttgatgttgt
     5761 tagtgttaat gaaggttttc ttttatccaa tacattagtc actctatgat gcgaggagat
     5821 gagaataagt gtttcaatat aaaggtattc gtattcgaaa tcgacataaa tatgcttcag
     5881 aacgatttat tttttatctt atgttgccgg ttctgttgct aatgagtgga tttttttctc
     5941 tcttttatac aacgatcaca tgggaatggt gctgtattta ttggataatc gcatttggtt
     6001 tagctggtct atttgcgtat atgtgtttgt atttttactt ttataatgtt tatcagcgat
     6061 tggttcataa aggaaaatta gcaaagatga tacatagtcg tcgattgatt cttgaaagag
     6121 tattgaaaaa tggacggcgg aaagtggtgt attatccaaa aattttttat cactataaaa
     6181 atggcttaat tcatgttcga ttgccattag atttaggtcc ctttcaagat cggtttaatg
     6241 agatggctgg gcaactagaa gaagcctttt tttgtgattt agtagaagag caacgagaga
     6301 aaaactatat ttgttatacc tttttatacg atgtggaaaa aaatcgtatt gggattcgag
     6361 atataaccgt aaaagatggc ataatcacct taatgaagaa tgttcaatgg gcatttaata
     6421 aagcaccaca tgccataatt ataggaagta ctggcggtgg gaaaacgtat tttataatgt
     6481 ctttgatcta tgcattatta ccgtatggta cgtttgatat ttgtgatcca aaacgtgcag
     6541 atttggctag tttagaaaaa ataccaattt ttaaagatca agttttttat ggtggtggta
     6601 ttttatcttg tttaataaat ctagctaaag aaactgctaa tcgatacgat tttttaagag
     6661 aacagcccga gtatgaatta gggtacgatt atacttatta tcatctaact cctcattttt
     6721 tggtgattga tgaatgggca gcatattatg catcgttaga tactaaagaa aaaaagaagg
     6781 caatgggatg tttgaaccaa attgctttaa tgggtcgtca atgtggcgtg tttttaattt
     6841 taggtaccca acgtccagat acaaagtatt tggacggcgc gatacgagat aattttggtt
     6901 tacgtgtaac actgggaaga gtatccaaag ctgggtataa gatgatcttt gaacatacag
     6961 taaaatcatt atttaataag ccgattaaag gtcgtggtta tattgattcg ggaaccgatc
     7021 aagtacgaga attttatgcg ccatttatcg atataacaag atttaacttt tttgcggaat
     7081 ttaaacaact atctgatcaa ttaatggctg aaaaaatcaa ggagacgaaa gagggaaaaa
     7141 atgtatgaag aaatcccagc tacatgtgga actcgattag aaataaaaga tttaatgatt
     7201 gactcttttt tggcaattgt tttcagtgag aagagattaa gcttctttat caataaaaaa
     7261 gtagagaatc gttggagatt agaacgtatg tatccaacga gttaactgat tggttacagt
     7321 tgccttatga caattatcga aggcgaatat gacgggtttt tacttcaaat aaaagtgctc
     7381 gatcctatag tgaccaaatt gtcgaaaaag cagcggaaca gcctgttttt tatggacgat
     7441 tcaaaggact ttcctgttct tatgttaaaa gtaaagaccg gtctcgactg tacctacaag
     7501 tagaggatat taacttgtcc tttgcgttgt cagaaaatta aatttgtcct cattagaata
     7561 cggaagatgc tgtgcgtgtg ctccacaagc aaacaagcct cagggcggaa ggacgcggaa
     7621 gcgtcctagc gttagcgaaa gccctgaggt gcagggtgcg ttgtggggcg tgcgcgtagc
     7681 gcacgcctaa aacccccata ttctaacagg ggggtagaat ttatattcgg tggcaaagat
     7741 gggtgattga aacattgata taacactatt ttgcttcaaa aattttgtga tatttttctt
     7801 cgctttgtaa cataaaatat cacaaaagaa aggaaagata tatttggatg ggacacaatt
     7861 aaagcaaatg cggttaagtc ggaatttgac gcagaaatta gtggcagata cattaggatg
     7921 gccacaatct tttttatcaa tgatagaaaa tgaccaacga agttgctcga aagaacaaga
     7981 agaagcactt tgtgaatttt atgcgaattt ttcaagtttt acgagtttag aaattaaatt
     8041 tgattatgtc cgtgtacgag ttccgacaaa tgatacgaaa acagttgtgg aagaaatttt
     8101 gggaatggat tttaactctt ttttttatgc agaaacccgc ttatatggtt atattgaaat
     8161 gtatcaaaaa ggcatgatcc gtgtattaaa ttctaaaaaa ggtgacgata aaggagtgtt
     8221 gatcgaatta tctggtcaag gctgtcggaa ttatgaaagt gtactggatg aacgaaatga
     8281 tagttggtat caattcttta cacgatgtgt ctctttcaat ggtgtgcctc gaagaattga
     8341 tattgcggta gatgattttg aagaactatt ttctttacat gaatttgcac aaaaactaaa
     8401 tcgtggcgaa tttgattcaa aatttcgaac ctggcggttt caagaagaaa aacagttgtt
     8461 agataatcta agtagtggct tatctgttta tttaggtagt tcacaaagtt tgatgtattt
     8521 ttgtttttat cagaagaatt atgaaattgc aaaaaagcag aaacttccgt tggaagaggt
     8581 cgaggtaaaa aatcgttatg aaattcgctt acgtgatgca aaagcggtca gttttattga
     8641 acaatactta aataaagtag atttttcaga tttaacgatg gggattttag ccaatcagtt
     8701 aataatgttt gagggagaga aaaacgcaaa tgtgtggaaa ccatggggaa aattgattca
     8761 gaacgttgga aaagtcaatt tgacaataac accagaaaag ccaaattata atcggaaatt
     8821 gatgtattta aagacacaac ctgctcgtac aatgaagatt gtaaaaactg tggatgaaat
     8881 tttaggattg aatcgatttg gagaaatttt agaagaaaca gaattacggg aacaagatga
     8941 aaaaattgta gaagatgagg tgcgtgctgt atatgaatat atacaagaaa atccagacgc
     9001 gattcattat ttgctataaa gcttgcgata agatggggga aacatgatta aaacagaaga
     9061 ccaatgatgg ggagagtata tgattgcttg ttaacaatta ataaaagaaa cgatcctatg
     9121 tgttgtaaat gatctacagt tatttaaaga atatttagat tatcaacaat caatcaaaat
     9181 gttacatgta gaaatagggg aagtattaag ctaccgtatt agtgattcgt taaaatattc
     9241 gttgacaaag atttatttat atgcaacaca agtatgtgga tatacgaaaa agcagtaaaa
     9301 acaatgatga aatatgacga tttgccaagc aagatatcct agatttacaa caagtattgg
     9361 aagaaaaact gtatctatat atgaaaaata gaggggaaaa gaaatgattc gatttttatt
     9421 ttctgtaggt tggcattttt tgaaattact gtt
//
LOCUS       DAXWLF010000104         9434 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXWLF010000104 DAXWLF010000000
VERSION     DAXWLF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.104"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            31..1290
                     /locus_tag="ACGE4U_001897"
     CDS             31..1290
                     /locus_tag="ACGE4U_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8394030.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            1300..2172
                     /locus_tag="ACGE4U_001898"
     CDS             1300..2172
                     /locus_tag="ACGE4U_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8394031.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            2184..3014
                     /locus_tag="ACGE4U_001899"
     CDS             2184..3014
                     /locus_tag="ACGE4U_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8394032.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            3054..5237
                     /locus_tag="ACGE4U_001900"
     CDS             3054..5237
                     /locus_tag="ACGE4U_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFF8394033.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            5298..6518
                     /locus_tag="ACGE4U_001901"
     CDS             5298..6518
                     /locus_tag="ACGE4U_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFF8394034.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            6515..7975
                     /gene="gtfA"
                     /locus_tag="ACGE4U_001902"
     CDS             6515..7975
                     /gene="gtfA"
                     /locus_tag="ACGE4U_001902"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFF8394035.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            8052..9158
                     /locus_tag="ACGE4U_001903"
                     /pseudo
     CDS             8052..9158
                     /locus_tag="ACGE4U_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         3184 a         1469 c         1851 g         2930 t
ORIGIN      
        1 gcattcaaat tgtataagga ggagcataaa atgaagtggt ctaaaaaaat tattttagct
       61 tgtatagcac taggagcaac gatctctttg actgcttgtg gaaaaaatca ggaagatgca
      121 agcggtaagg tagaaataga gttttttaat caaaaaaaag aaatgactca aacgattcag
      181 gagattgcta aagattttga agcaaaaaac cctgatattc atgtaaaagt tgtggatgta
      241 ccgaatgctg gtgaagtaat taaaacacgt atgcttgcag gcgatgtacc agatgtcatt
      301 aatctttatc ctcaaagtat agaattgaaa gaatgggcga aagctggtta tttagaggac
      361 ttaaccgaag aaccgtattt agagaatatc aaaaatggat atgcacaacg ttttgctatt
      421 gaggatcgtg tatatagcat accattaacc gctaatgtct atggttttta ctataacaaa
      481 actgcttttg aggaaatggg gattcaagca ccagaaactt gggcagaatt tgaaaaaata
      541 gtagctgaga ttaaagatca aaacaaagta ccgtttgcga ttgcaggagc agaaggctgg
      601 actttaaatg gttatcacca actagctata gcgactgctg ctggtggtga agaggaagcg
      661 aacaatgttt ggcgtttctc agaagttaat ggaattaatg ctgaaagcaa agaaatgcaa
      721 aaagatttta atcgattaga tttgttaaga gaaccaaaag ctttgcaaaa caactggcaa
      781 ggtgccggtt ataacgatac agtagttact tttacaaaag gtgatgcgtt gattatgccg
      841 aatggttcat gggcaatgcc gatgattcat tcacaaaatc cagattttga agtaggaact
      901 tttccttttc cagcagatga agcaggtcaa agtctaacga ttggcgcagg agacctagcc
      961 ttatcagttt ctgcaacttc tcaacataaa gaagaagcca aacgttttgt agaatatatg
     1021 acaacacctg aagcgatgca gaaatattat gatgtagatg gtgcaccgtg tgctgttgag
     1081 ggcgttatcg agaatactca ggattcacca ttaagtggtt taactgaact tgcttttact
     1141 gatcgtcatt tagtttggtt agccaaagat tggaatagtg aaaatgattt ttatacctta
     1201 acaacaaatt atttacacaa tggcaatcaa aaagccatga ttaacgcatt aaatgctttc
     1261 tttaatccaa tgaaagctga cggaaggtag gaaaataaaa tgaaaataaa agaaatttta
     1321 aatcgttact gggcaatcat ttttgtagca attccaatta ttttgcagat gatttttttc
     1381 tactttccaa tggttcaagg tgctttttac agtttaacta attggactgg tctgacttat
     1441 aattttgatt ttgtgggttt gaacaattat aagattcttt tttctgatcc gaagtttatc
     1501 aaatcgattg gcttcacttt aattgtgacg ctttgcatga ttgctggaca aattggtttt
     1561 ggtattttga ttgcgcgggc gttgaacgca aatattaaag ggaaaacatt ctttagagca
     1621 tggttctttt ttccagcggt cttatcgggc gtggttgttt ctttagtatt taaacaaatt
     1681 tttaactatg gtttaccaaa aattggtagt atgttaggca ttgaattttt acaaaccagt
     1741 cttttaggaa caacatgggg agcgattttt gcagtaattt ttgttttact ttggcaagga
     1801 attgccatgc cagtcatcat tttcttagcg ggttttcaaa gtattccaca agaaattatt
     1861 gaagctgcta gaatggatgg ggcgaatgat aagcaaattt tttggaatat tgaaatcacc
     1921 tacttattac caagtatttc aatggtattt attatggcat taaaaggtgg cttaacagca
     1981 tttgatcaaa tttttgcttt aactggtggt ggaccaagcg atgccacaac atctttgggg
     2041 ttacttgttt ataattatgc ttttaaaaac aatcagtttg gttatgcgaa tgctattgct
     2101 ttagtcttat ttatcttaat tgggattgtt tcactggcac aattaaaatt atcgaaaaaa
     2161 tttgaaatct aggaggtttc ccaatgaaaa catcaaaaga aaaaaatatt tctaaatact
     2221 tgcttttatt attagggggc ttcatgattt tagttccctt agtttcaacg ctattcggtt
     2281 catttaaaac aacacccgat attatgaata atttctttcg ttttcctaat ccagcgactt
     2341 tggaaaattt ccgaagattg ttagcggatg gaattggtca ttacttttgg aattcggcaa
     2401 tcatcacaat cattgcagta gttgctgtga cagcaattat tcctatggct gcttattcac
     2461 tagcaagaaa aatgactcgt gggaaagcat acggattgat gtatttattt ttaattttag
     2521 gaatttttgt tcctttccaa gtaattatga ttccgattac cgttatgatg agtaaaattg
     2581 gtttagcaaa tatgtgggga ttaatcttac tctatctcac ttatgccgta ccacagacac
     2641 tatttttata tgttggtttt attaaactaa atgtgcctat cagtctggat gaggctgctg
     2701 aaattgatgg ggctagtcac tttacaactt atttcaaaat tgttttccca atgttaaaac
     2761 caatgcatgc gacgactatt attcttaatg ctctatggtt ttggaatgat ttcatgctac
     2821 cattattaat tttaaatcgt gattctaata tgtggacact accattgttt caatataatt
     2881 atacgggaca atattttaac gactatggtc caagttttgc ttcctataca gtaggaattg
     2941 tcacgattac actagtttat cttgctttcc aaagaagtat tatagctgga atgagcaatg
     3001 gagcagtgaa ataaccaata aattattctc attataaagg agttcataaa cttatgatta
     3061 tatttgacaa tcacacacaa acatttcatt tacaaaataa aaaaattagc tatctattag
     3121 gaatcgaaga aaataattat ttaacgcatc tttattttgg aaaaaagatt acgcactatt
     3181 ctggtgggta tcgctacccc cgaacagatc gttccttctc acccaacaat gcaaatgcgc
     3241 aagatcgttt attatcttta gacacattac cattagaatt tccaggatat ggacatggtg
     3301 attttagaga accggcgcat aatattaaat taggaaacgg tagtcgtgta aatgattttc
     3361 gttatgatag ctacgaaatt caaaaaggaa agcccagctt agcgggattg ccagccttat
     3421 atacggaaga tgatgatgaa gctgaaacgc tgattattca attgaaagac gcgattagtg
     3481 gattgacttt acagttgttc tacacaattt atcaaaatca cagtgtaatt attcgttcta
     3541 cgcaattagt aaataatagt gatgaaacag tggagattaa ccgcttagct agtcaatcaa
     3601 ttgattttcc aaatcgtcca ctcgagttaa ttcatttaaa tggtacctgg gcaagagaac
     3661 gtcaaatgac tagagaaaaa attcatacag ggataaaagt attagatagt aaacgcggat
     3721 caagcagcca tcaacaaaat ccgtttgttg ctttagtaga tgccaaaaca gatgaatttc
     3781 aaggagaggc atatggattt aatttagttt atagcggtaa tcacgaaacg gtgattcaaa
     3841 aagatccatt tgaccaaaca cgggtcatta tgggaattaa ttcatttaat tttggttggc
     3901 aactaaaaac tggcgactca tttcaaacgc cagaagtcgt gatggtttat agtaatgaag
     3961 gtctgaatac gttatcacag acgtatcatg aattgtataa tcaccgttta attcgtggaa
     4021 actatcgttt aaaagaacgt cccacgttaa tcaacaactg ggaagcaacg tattttgatt
     4081 ttaacgaaga aaaaatcatg aatattgtcg atgatgcgaa gaacttgggc attgaaatgt
     4141 ttgttttaga tgatggttgg tttggcggtc gtgaaaatga cttcacttca ttaggtgatt
     4201 gggttgaaac agaaggcaaa cttgcttgtg gtttggaaaa tttagccaag caagtgcatg
     4261 ataacgggat gaaatttggg atttggtttg aaccggaaat gatttctgaa gatagtgaac
     4321 tatttcgagc acatccagat tgggcattgg gtattccagg tcgtgaccgc tcaattagtc
     4381 gaaatcaata tgttcttgat ttttcaagac aagatgttcg tgataatatc tatcaacaaa
     4441 tgacggcgat ttttgatcgt gtaccgattg attatgtgaa gtgggatatg aatcgaaata
     4501 tgacagaagt ctattctgtc ttgttggact caacgatgca aggtgaagtg gctcatcgtt
     4561 atatgttagg gttatatgaa tttttagaaa agttaacaac gaattatcca actattttat
     4621 ttgaaagttg ttcgggtgga ggcggtcgtt ttgatgcagg tttcttgtat tacatgccac
     4681 aaacttggac gagcgacaat accgatgccg tggctcgtct gaaaattcaa tatggaacca
     4741 gcttagtgta tccaatttca agtatgggtg cgcatgtttc agcagtccca aatcatcaaa
     4801 caggaagaga aacgagttta actattcgtg gcaatgctgc gatggctggt gtttttggtt
     4861 atgagctaga tctaacgcaa ttaacttctg aagaaaaagc agaaattaaa gaacaagttg
     4921 ccttttataa aaagcatcgc caacttttgc aatatggaac tttttatcgc ttggaaagtc
     4981 cgtttgaagg caatgatact gcatggatgt ttgtctcaaa agaccaaaca gaagccatgg
     5041 tcttttattt ccgagtatta gcagaagctg cgcatccatt agtcacattg aaattagcag
     5101 gattagcaga agaagcaact tatcaaatgg aagatgtaac atttgctggc gatgaattga
     5161 tgaatctcgg tttttatatt gactcagaac tacatggtga ttatgcgaca caacgctttt
     5221 atttgaaaca gatatagtag cttgaattta caatataagt gcaacaccaa aagagactca
     5281 ttccaaacga agaaaatatg ttttatttac acgcatttac taaaaagcag cctgatttga
     5341 attgggaaaa tgaagaagta cgtgaagaaa tctatgcaat gattaactat tggttagata
     5401 aaggactagg cggatttaga atcgatgcga tattaaacat caaaaaaaaa ctagagtatg
     5461 gcatctttga atctgatgga gaagatgggt tagccttcat tgggccctgg attcttaatc
     5521 agtcgggcat tgaagtgtgg ttaaaagaaa tgagagagcg gacatttaaa ccacataata
     5581 gtatgacggt agcagaagcc gatgttccta atgaacgttt agatgaatat attggtgaag
     5641 atggctatta ttcgatggtt tttgatttta gttatacgga tattgatgta cctgaaactg
     5701 gtgaatggtt taaagatagc caatggacat ggacggatat gcgtaccaat atttttacga
     5761 atcaattagt tacgcaagat aaaggttggg gcgccttata tttagaaaac cacgatcaac
     5821 cacgttcaat taataaatat attccagaag aatggatcaa tgattatagt aaaaaaatgt
     5881 tggcgacgct ttttatgtta ctaagaggta ccccttttat ttatcaagga caagaactag
     5941 gaatgaccaa catagaaatg gcatcattag aagattttga tgatgtagct acgcattctc
     6001 agtataaacg ggcattagaa tatggattgg caccagagca agcattgaaa gctgtcaata
     6061 aacgaagtcg ggataattct cgcacaccta tgcaatggaa tacgcaaaaa aatgccagat
     6121 tctcaacttc tgataatgta tggttaaaag taaatcctaa ttatccagaa ctcaatgcag
     6181 aagctcagtt aaccaatcaa cagtcggtat tcaattatta tcatcaatta attaaattaa
     6241 gaaaaaatag tgtatacaaa gacgtgctaa tctacggaca atttctacca gtgaaagata
     6301 aaaatgaaca tattttgctt tatgaacgcc aattagaaga taaaatcgta ttagttatct
     6361 taaacgttac agcaacagaa caagaatatt atgtagatga acagtaccaa caagtattaa
     6421 tcaataatta tgaagatgtt ttattagatc aaagtcgaaa attgcgttta cgaccgtttg
     6481 aaagtattgt attagctaat tatggaggac aattatgaaa attaaaaacg aagcaatgct
     6541 catcacctat gcagatagtt taggaaaaaa catgggcgaa ttaaaacaag tcttagacaa
     6601 acatttacaa ggtgttattg gtggtgtaca tctgttgcct ttttttccat cgacaggtga
     6661 tcgaggcttt gcgccgagtg attatacgac agttgatcca gctcttggaa catgggaaga
     6721 tgtggaagca ttaggtgaag attattattt gatgtttgat tttatgatta atcacatttc
     6781 tcgtgaatca aaatttttcc aagatttcaa aaaaaaccat gagcagtcac catacaaaga
     6841 aatgtttatc cgtattcatg aatttttccc agaaaatcga ccaacacaag aagatattga
     6901 tttaatctac aagcgtaaag acaaagcgcc gtttcaaacg gtagaatttg cggatggtac
     6961 gacagaagag gtttggaata cctttgggga agaacaaatt gatttagatg tgaccaaaga
     7021 agttgtgaaa gaatttattc gagaaaccat caaagatatg gcgaaccatg gctgttcttt
     7081 aattcgatta gatgcgtttg cttatgcgat taaaaaatta gataccaatg atttcttcgt
     7141 ggaaccagat atttgggaac ttttagacga agtgcgtcaa gaagcagcga aatatgacgt
     7201 cgaattatta ccagaaatcc atgagcatta ttccattcaa atgaaaattg ccaatcatga
     7261 ttattatatc tatgacttcg ccttgccaat ggtcactctg tattcacttt atagtggaaa
     7321 atcagaacgt ttagccaatt ggttgaaaat gagtccgatg aagcagttta caacattgga
     7381 tacgcatgat ggaattggtg ttgtggacgc tcgtgattta ttaacagatg aagaattgga
     7441 ttacacctct gaggaattgt ataaagtcgg ggcgaatgtg aagaaggtct attcaagtgc
     7501 caattacaat aatttagata tttatcaaat taacagcacc tattatagtg cattaggcga
     7561 taatgaccgt agctatctgt tagcacgtgc gattcaagta tttgcgccag ggattccaca
     7621 aatttattac gttgggttat tagcaggaaa aaatgatatt gaactgttgg aagcaacgaa
     7681 agaagggcgt aatattaatc gtcattacta tgatttagcc gaaattgaag aagaagtgca
     7741 acggccagtg attcaacaat tatttgcact tttaaaattt agaaatgaat ctgcggcgtt
     7801 tgatttggat ggaacgattg aagtagcaac gccatcagaa tcagaaatag tgattacacg
     7861 taaaaataaa aacggtagtg atagtgcaac attaacggct aacttggata cgaaagaatt
     7921 tatcattgag tctaaagata taaaaatttt cactagtgtt gcattaaaat tttaacctat
     7981 tccatagaac aaaaaatcct ttatactagc acttggatga cttatccaag accaataaaa
     8041 aggactcata catggataag tataaaacaa atacagcatt taataaatgg ttttctgcca
     8101 taaaattagg aaaactttct gtcaactccc aaaaatgtat ttttgattac aattcgtatc
     8161 gtaaaaaact gtcttttgaa gctgtgctca agctctttct ctacgcagtt aatgaagaaa
     8221 aagaaagttt gcgtgatttg agtacagctc ttatcgaccg atcgctccaa ctagaaaccg
     8281 atgtgtcatc gattagtcat acccaattgt ctcgagcatt taatgcactt gaacctaaag
     8341 tgttagaaga aatttttcaa caactgttag aaaaagttca acatcaaatc aaaccaacca
     8401 agcgaaatca tctgtatctg attgattcca gtacgttttc gcttagttta aaacgtcata
     8461 agtgggcgac tttccgtcaa acaaaatcag gtgtcaagtt acacttaaac tattgttata
     8521 tggacgatac tacgatgtat ccaactgatt ttaccttaac aaatgcccat gaacatgatc
     8581 tcaatcaact accagtattg gtgaatcaac cagaggcaac ttacgtgttt gatcgtgggt
     8641 attaggactt tgaaaagatg gatcagatgc attgggatgg ctatttcttc gtcactcgga
     8701 ttaaaaaaaa caccaaagtt cacgtgatag atactctgga aacatcgcca gaaacagaaa
     8761 ttctacgaga cgagctggtt cgtttaggat ctaaaaccta tctaaccgcc aatttccgac
     8821 tagtcactgt tcaagataag aacggacgtg tgtttcaatt tatcaccaat cgaatggacg
     8881 tctcttcaaa agaaatctct gatatgtatc acgctcgttg gcaaatcgaa ttgtttttca
     8941 agcacatcaa acaacacatg accattaaaa ccttcttttc tcaaagtgaa aaaggcgtgc
     9001 agaatcaatt gattttaacg atgatttccg ctctcctcac gtttctcatc aaattggaga
     9061 cgaaaacgga aaaatcagtg ttccaaatca aacgattctt tcgttattta ctgtttcagc
     9121 cctttgaatg ttgtctagaa aaattaattc ccacatgaga aaaaagaaat ttggctcgtt
     9181 acttttggtt ttcagttgaa taacagataa aaaattttct ggaaaaagtc atcctctgcg
     9241 tcagcacagt tttttttgac ttattttcag aggaaatagt tttcagaata ttctaaaatg
     9301 ttttatgcaa cactagtgat attttttaga attagatttc taatgtgcat tttttgagta
     9361 ttaaggagca agtatacaaa gatttataaa gctgattaaa tttgaataaa aatatgcagg
     9421 aacgctacta tttt
//
LOCUS       DAXWLF010000105         9373 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXWLF010000105 DAXWLF010000000
VERSION     DAXWLF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.105"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(11..1639)
                     /locus_tag="ACGE4U_001904"
     CDS             complement(11..1639)
                     /locus_tag="ACGE4U_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394036.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            complement(1659..1826)
                     /locus_tag="ACGE4U_001905"
     CDS             complement(1659..1826)
                     /locus_tag="ACGE4U_001905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394037.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            complement(1863..2282)
                     /locus_tag="ACGE4U_001906"
     CDS             complement(1863..2282)
                     /locus_tag="ACGE4U_001906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394038.1"
                     /translation="MISGKYDYAFDFEGEKDYKSVAVFQSDFEKLKNYAGRRNVNFAR
                     LLEEFYSEELGVGNEYKRETYAKLSHKLSQALKEVTEVKAELDAKLEEVKKYQTERKN
                     NMKNQVEFDEQKEFEKARKRYNKIMNELSYEHLTVHE"
     gene            2353..3494
                     /locus_tag="ACGE4U_001907"
     CDS             join(2353..2581,2581..3494)
                     /locus_tag="ACGE4U_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8394039.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            3713..4084
                     /locus_tag="ACGE4U_001908"
     CDS             3713..4084
                     /locus_tag="ACGE4U_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394040.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            4169..4369
                     /locus_tag="ACGE4U_001909"
     CDS             4169..4369
                     /locus_tag="ACGE4U_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394041.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            4503..5729
                     /locus_tag="ACGE4U_001910"
     CDS             4503..5729
                     /locus_tag="ACGE4U_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394042.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            6326..6721
                     /locus_tag="ACGE4U_001911"
                     /pseudo
     CDS             6326..6721
                     /locus_tag="ACGE4U_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            6799..7838
                     /locus_tag="ACGE4U_001912"
     CDS             join(6799..7296,7299..7838)
                     /locus_tag="ACGE4U_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFF8394043.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGT
                     LDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEE
                     NARRLALIFLPPYSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERP
                     LEVIDRLCVRM"
     gene            7851..8789
                     /locus_tag="ACGE4U_001913"
                     /pseudo
     CDS             7851..8789
                     /locus_tag="ACGE4U_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            8782..9129
                     /locus_tag="ACGE4U_001914"
     CDS             8782..9129
                     /locus_tag="ACGE4U_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394044.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            9126..9308
                     /locus_tag="ACGE4U_001915"
     CDS             9126..9308
                     /locus_tag="ACGE4U_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394045.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
BASE COUNT         3257 a         1551 c         1669 g         2896 t
ORIGIN      
        1 acttctctct ttatataagt gataaaagat cgtaaatacg tttaattgtt aagttcatat
       61 tcacatgatt atccaaataa aatatcttat ctttattaga aatttcactc ataacacgat
      121 aatatagacc atgattcaag taatctgtat atagaaaaat cgcatcacac gtattcaaat
      181 ttttaagtat ctttccatta ttttcgctta catcaaacat tttaatattt ggtaatagtt
      241 cagttaattt attttgaaaa tttacatgtc ctcccactaa aagaatgttt ttatgtttta
      301 gataagctat catttcagac agtgaaggct cttcagtttt accatttaat gattgtctat
      361 gttcttgctc ctgcaattgc tttaatactt tattttcaaa cctcagctgt tcaagttctt
      421 ttctgattcc ttttaattct ttaatctctt gcttcaaaag ttcctcttct ttttttgccg
      481 atttgactaa agattctaac cgaccgtttt ctttactcgc aatacttaat tcttttctta
      541 atttgattac ttcagatttg ttttcttttc taatacttgc aatttgtttt gctatttcat
      601 aatcagaatt caggatcaat tcttccttag catttttaaa caaccgagtt aaaacgaact
      661 gatataacaa actataaaaa actagcaaat agtcaatttg tcggtactcg gtattctgat
      721 caattgcata tagagagatt aaaacgaaat acacaatatc attcagctcg ctacccttga
      781 tctcaaaatt attgactaag taactatcaa tacccatcaa atctgcaaaa tctccataca
      841 aagaaaaata tgattttgac aatatatcta tatctgaatc gtcaaaccca tttactattt
      901 tcccatcatt gttagtagtt gctaacgaag caatagtgac ttttgaattg ggttttacaa
      961 atcctatacc taatagttct ttcatctttt taatttcaag tttgttatcc ttttgttcgg
     1021 ctttatgctg tttcatattg taaagcggat aaaatgctgt tccgtaagca ccttttagta
     1081 cgttatctat aaaataatct tcagaacctg ttttgaatag aaagttaatc gcagccaaat
     1141 tagataacag ctcttcagtg ttgtgcatca attttttatc atttaataaa ttatccccaa
     1201 gatatttcat tgcataataa gggtttgttt cgctacttat ttttaatatt ttttgatagc
     1261 tattcctcac gatattaacc aaaatatctg tctgattaat tgaattacag tacaaagcca
     1321 ctcctaatgc catataaaca ttcaaatcat gttttgccgt accgttgaat aaaacgtctt
     1381 caacttttga attatctcgt ctatactttt tgatcatatt tttgatattt tgatcattct
     1441 cttggtatat ttttttcact cgttccaatg ccttgggttt gttggaaaca gcaacagcga
     1501 agtctagttc tttgaaatca atatttagta tctgtacctc atcacctaga ttataatctc
     1561 tatttacgct cggaaaactg ctgaaatcta actgtttttt acctattttt ggcgaaaata
     1621 ttccttcgat ttctggcaaa tgtaatcaca ctctctcctt atttatcttt agaaaatttg
     1681 tcactattct ccttatattt atcaataaac ctttttaatc gagcaatata agcgttaacc
     1741 cttttaggtt cttcttttcg ccattcatcg tttacgtgac cattttcaaa gaaagaagct
     1801 aaaagagttt ccacttcatt aaccatatct gcgatcgtat atttttctgt accctcactc
     1861 aattattcat gaactgttaa gtgttcatag ctaagttcat tcataatttt gttatagcgt
     1921 tttcttgctt tttcaaattc tttctgttcg tcaaattcca cttgattctt catgttgttc
     1981 ttcctctctg tttgatattt tttcacttct tcaagtttag catctagttc cgctttaact
     2041 tctgtcactt ctttaagcgc ttgggataac ttatgagaca atttcgcata agtttctctc
     2101 ttgtattcat ttcctactcc caactcttcg gagtagaact cttctagtag tcttgcaaaa
     2161 ttaacatttc tcctgccagc atagtttttt agcttctcaa aatcagattg aaagacagca
     2221 acacttttgt aatccttttc accctcaaaa tcaaaagcat agtcatactt tcctgaaatc
     2281 aatttgatgt taggatgatt tcatggacac gatttggtag aatctaacta ctaagaaaag
     2341 gacgtgtcca cgatggcaaa agccaagaaa aaatttgatg aagactttaa gaagatgatc
     2401 ttagatttaa accagtctgg ccaatcggtc gaagaactgg cagcgcaata tggtatcgct
     2461 acacaaacga tttatcgttg gaagaagctt catacaaaga atgtagcaac cggcatgact
     2521 gaggcagaaa tcctagcgat gaaaaaggaa atggcccgca tgcaggagga aaacactatc
     2581 ctaaaaaagg ctttaaccat attcgctcaa aagtaaaaat gagtgatgtc ttcgaattta
     2641 ttcaatcaaa tgttcatgat catgacgtaa aacaaatgtg taccgttttg gaagcaccga
     2701 aatccagcta ctatgactgg aagaaaaaga aaccttccaa gcgccaatca gaaaacaaac
     2761 gcttaaaccg cttgatttca ggaatctact ttgaaaacaa gggtatctat ggtgcgccta
     2821 aaattcataa gattcttgtt ggccgtggcg agaccttgtc cttaaagaaa gtacaaatac
     2881 tgatgagaaa attaggcttg cgttcagtca ccctcaagaa gtacaagcct ggcaaacaag
     2941 cgaaagttaa aagtgaagga cgtaaaaacc tgttaaacca agattttacc actacgtcga
     3001 tcaatcaaaa atgggtgaca gatattacct atattcatac cctaaaagat ggctggacct
     3061 acctctccac tattcaagac ctccacacaa agaaagtcat cggttggaag tttggcaagc
     3121 agatgacgaa agaattggtt atcgaaaccc ttgacaatgc gctcttaaac caaacaccct
     3181 ccgagaactt gatcatccac tcagatttgg gttctcagta cactagcgaa gcctatgagg
     3241 caaagctgaa tgaattgaac attcggcact ccttcagtcg taagggctgt ccctacgata
     3301 atgccggtat cgaatcgttt cacgcctcac tcaaaaaaga agaagtctat ccatcaaaag
     3361 cgtatgaaaa ctttgaagct gcttatttag cgttatttca atacattgaa ggattttaca
     3421 atcgcaagcg aatccatagt agtatcaact atttaacacc aaatcagatg gaggagttgg
     3481 cattgcaagc ttagttccta gctaccactt acattttttt ggcgagattt ccacagggtt
     3541 tcaacttcat cgcttcgatc agcggtcaaa cggttttggt ttgaccgctg gtcgaaacga
     3601 tgagatactc acccgctgga atctcgaaaa aattctcaca aatcgtgtcc gaggtattga
     3661 ctcaaatcca atgataaata ggacacaaaa tttggaaagt aggtaagtca ctatgagaga
     3721 actaacttta tcttctaagg aattagaaaa gaaaactgct ttattaaacg aaatatttag
     3781 tgctgattgt ataatccaaa gtttaaaaga aagttcattt tcaaaactaa cagatcagga
     3841 aaaaatttct tatatgctaa gtttagataa tctaggaact aaatatgatg aatcagcaag
     3901 tagcgataag cttttgattg aattagtatc aagtcttagt agttttggaa aaatgaaaag
     3961 atttaatgtg atattagaaa tttttgcttg gttaatccct tcatttattt ggttttttta
     4021 tattttcata gttgaatctg agttaatagc caaggtctct cttggactgt taattccgat
     4081 ataaataggt tcagcaattt actttaataa cttgtataca cctattcaaa taaaaaagca
     4141 gttattacat gaaggattaa gtggtaatat gttaaaaaga attgcatggg ataaagataa
     4201 taaaacagat attgagctta aagaatataa atacaattat ttctcgagat gtatagatag
     4261 aaagttactg tttaaaaaag aaggattaga atttagtttt gatgaagatg aatttgaaaa
     4321 attagtgatg gactggcgta agaatttact ggatacggta caattctaaa agcaattgag
     4381 gtcgaaattc cgatacttga atcatgatct atattatcgt gttatgagtg aaatttctaa
     4441 taaagataag atatttcatt tggacagtca tgtgaatatg agcttaacaa ttaaacgtat
     4501 ttatgatctt ttatatgttt ataaacatta atgaggtgct aaaattgaca acaataacta
     4561 tgacaaaaaa aatagaagta tatgttgatg gatcacaaga agaaagggat tccttttttg
     4621 attttttttg gaaaaatagt agagaatatg taaaagcgtt gaatctggta tataaacagc
     4681 taatgtttga agagttgata aaagatgcaa ttaaagaaat ggatcaaggt tatgctgaga
     4741 gagaagaaag tattcaaaat cggattgaaa agttagcata tactgatcca atagatcaaa
     4801 aaaaacttga taaagcaaaa gctgaattat ctaaattgag aaatgaaaaa aataaagaag
     4861 cgagagaaat attaacgcaa gcgatagggt tgaagagtca aacgagaaca cgagatgtag
     4921 caaaaaaatt agactttgac tttggagatt ttgttgattc cgcaaatatg aaagctagta
     4981 aagattttgg caatgatttt atcggaattg tgaccggaca aagaacgcct agaatatata
     5041 aaaatagaaa caaatatgga ttattacctt ttagaggtcg agatattaaa atccataaag
     5101 atgataagtt ttatatttat ttacctaaag gctttaggtt taaagtgaat ttaggtagca
     5161 agccaagaaa agcaagagat gttgcttcag ttttacaaca cattatggat ggcgattaca
     5221 aattaaatca aagtaatctt atttttgatg gaagaaaatt attttttaat ctaacaattt
     5281 cttttgaaaa aagctataat gaaatggaat tcaagaaagg taatgaattg atcgtaaact
     5341 ttgcagattt gtgtagcggt gaatgtttaa ttaatgataa atctgtaatg tcttttggtg
     5401 atagccaata tataagaaac ttcaaaagta aaattttagc aatgaaatca aaagaacaaa
     5461 gccgaggagt cttttctaag ggcggtcatg gtcgaaaaag aaaattagat caagatcgat
     5521 gggaagcaat aaagaaaaga gaaagtaatt ttgttaaaac ttataataat caatgttctg
     5581 caaaaatcgt taaaactgct attcgcaata aagctcaaat tattcgtatc attgaaccag
     5641 ataaggctta tggcgaatgg gcttactatc aattgaaaga acaactagta aacaaaggaa
     5701 aaagatatgg gatagaagtc agaagttagt aaattaaaca atcgttgatt tggcaacgaa
     5761 atagtgaggg aaaatccact cactgaaggg taaactccgt caagttgtgt gaccagacgg
     5821 aaggacgaat gttgttgagg ttttagaacc atataaaaca gcatgtttct caaattttta
     5881 ggataaggga aattgctagc agtttgtttt agaaccatat aaaacagcat gtttctcaaa
     5941 tgagtacttt aaattaattg atggcgttcc tgctttagaa ccatataaaa caacatggat
     6001 ttaaaacaag aagtaagtca gttctccttt tactttagta aggaaaaata aagtattctc
     6061 aataaaactt aaaataagca aacctttgtt ttaaaatgat tgtcaagtta aaaaaatctg
     6121 ggatcatata aaatagtata gttctcaaat atgattagaa accctattaa agagcaatat
     6181 taagccaact tacttaaatc gtttaattaa gaacaataat ctcttttagt cagactttta
     6241 gtctgacttt tttgtatttc caaaatttat tctttacaaa aacgaacata cgtttgtata
     6301 attacttgtg aatgggggaa taaaaatgtt tgattacagt agagaaccgg tgaatgacta
     6361 ttttttaatc gacatgaagt ctttctatgc tagcgtggaa tgtattgaac gaaatttaga
     6421 cccaatgact acaccgttaa ttgtcatgag tagatcggac aacactggtt caggtctgac
     6481 tctagcttct tcacctactg caaagaaaaa atatcatatt actaatgtta gtagagctac
     6541 tgatttacct tatccattac caaaagaact tcatattgtt ccaccacgaa tgaacttata
     6601 tataaaaaaa aatatggaag ttaatcagat ttttagaaag tatgtcgcag atgaagattt
     6661 attgatttat tcgattgatg aatcaatttt aaaagtgaca aaatccttaa atttatttac
     6721 tatataaatt gagttaaatt gtattcgttt ttcctcctat aggcgtatac taaagaaaac
     6781 acttgaggag tggggaacat ggaaacagaa cgccaaatcc gtgaattgga aaaagcgatg
     6841 aatacgacag acaaccgccg gttgtacgag cgtttcctgg cagtcaagtt ggttcttgaa
     6901 ggccacaccc ggaaagaagc cgggcacacc atcggccgcg acgagcatac tgtcagccac
     6961 tatgtgaaaa actactgcca taatggaatc caaggtttag tctccaaaaa acaaagcgga
     7021 cgaccttcac ggctgacaga tgaacaggaa gcggaactgg tccaaatcgt cgcctaccac
     7081 actccggagg aagtaggttt caaatcccgt gccaattgga cattggcgat tgtgtgtgac
     7141 ctgattctcc gggaatggag ttatggatat acgcaaaaag gcgtggcgga cctccttcac
     7201 cggctaggac taagctggac ccgtccgacg tataccctca aaaaagcgga ttccaagaaa
     7261 caacaggtat ttttggaaga aaccttcccg agcctaaaaa aaaattgctg aatagcgata
     7321 ttgaccacct cctattcgag gacgaaacca tgatccggga ttaccaagcc attggctgca
     7381 cctggtttct ccgcggccac cagcgaaaaa tccccactta tggacaacac aaaggggtaa
     7441 aattgatagg caccttggat tatgaaaccg gtcggatcat ttgtacagaa gaaggggcat
     7501 acgacgcagc ggccttcctc cgttttctgg agcggacgtt gcgggaatac cccaccggaa
     7561 aaatcgtgat ggtattggat aatgccctta tccaccacgc caagctgatc cagtcttttc
     7621 ttgaagaaaa cgctaggcgc ctggcactca tcttcctccc tccctacagc cctcagttga
     7681 acctaatgga aggcgtttgg aagtggctca aggaatcggt catcaacaat gttttttttg
     7741 accacgtcca gaaaatcaaa cagtcggtcc gcggtttctt agccgatgta aacgagcgtc
     7801 cccttgaggt gattgaccga ctgtgcgtgc ggatgtaaag tatttaattc aacctatata
     7861 gttcaggaac aagaagcgag agaagaagag aattagctaa aataatcaaa gatgagattc
     7921 agaaagaaac aggtctaata gcaacagtcg gaataggaga taacccatta ttagctaaac
     7981 ttgcactgga caatgaagcg aagagtagta aagaatttat agctgagtgg acttatgaga
     8041 ctgtacctga aaaaatatgg aaaataaacg agatgactga cttttggggc attggatcac
     8101 gcatgaaaaa aagattgaat catatgggaa ttatgagtat agaagattta gcaaattgga
     8161 atccctacac aattaaaagt cagttaggtg taatcggact ccagttatat ttccatacca
     8221 atggaattga tcgtacggat attgctattc caccagaacc aaccaaagaa aagtcttatg
     8281 ggaattcaca agttctacca aaagattata cgaggagaga agaagtagaa ctagtagtca
     8341 aggagatggc agagcaagta gccattcgaa ttagacaaca caattgcaaa actggttgtg
     8401 tacgcttagc aattggtacg tcaattttgg aagaaaaatc aggtttctcg caccagatga
     8461 aaataccaat tacagataat acgaaagaat tacaaaatta ttgtcttttt ttattcaata
     8521 agttttatga ggggcaggaa gtacgacata taggagtaac ttatagcaga ttagtataca
     8581 cagattccct acaattagat ttatttagtg atccaagaaa aaagattgct gaagaaaatt
     8641 tggataggat tattgataag attagacaaa aatatggatt tacagctatt atacatgctt
     8701 catctatgtt agaaggtgca agaagtatta cgagaagcac tttaatagga ggacacgcag
     8761 gaggattagg aggtttgcat aatgattgaa tataacgata gaggacgttt aaaatggtcg
     8821 ggattttatt tgtctgatca tactgaaaga atcaatgcag acaatgtaca acgtgctaat
     8881 cataatttgg caaaagaaca aatgacgact gaagagattg cgaaaatact gagaactgct
     8941 gtcataaaaa atagacccgt ctgcattcaa aaagaggaac gaaacgctga aggttactat
     9001 ccagatgatg tagtaggaaa aattattgga tatgacgagc ttggtgtatt tgtggaagaa
     9061 gaaaaagttc actatgatga aattaggaat gtttctttct cggaattatt aaaatggagt
     9121 cagatatgaa cattattaaa aaatacgaat tcggttatat tacgtatgaa cagctaatag
     9181 aggaattatg gggatatggt caacaattaa ttaatgaagt ggggattgat tgcttttgtt
     9241 tctatattga atccggagca ggataccata agaacagata ctatatattt ccatatccac
     9301 caaaatagtc tcttagatat ttttaaattt tctccaaata ctttcaatta ttgtgaaata
     9361 tttcccttta att
//
LOCUS       DAXWLF010000106         9309 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXWLF010000106 DAXWLF010000000
VERSION     DAXWLF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.106"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            21..1616
                     /locus_tag="ACGE4U_001916"
     CDS             21..1616
                     /locus_tag="ACGE4U_001916"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8394046.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            1606..2805
                     /locus_tag="ACGE4U_001917"
     CDS             1606..2805
                     /locus_tag="ACGE4U_001917"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFF8394047.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            2818..5967
                     /locus_tag="ACGE4U_001918"
     CDS             2818..5967
                     /locus_tag="ACGE4U_001918"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFF8394048.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(7902..8567)
                     /locus_tag="ACGE4U_001919"
     CDS             complement(7902..8567)
                     /locus_tag="ACGE4U_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394049.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            8693..>9309
                     /locus_tag="ACGE4U_001920"
     CDS             8693..>9309
                     /locus_tag="ACGE4U_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFF8394050.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGL"
BASE COUNT         3201 a         1502 c         1845 g         2761 t
ORIGIN      
        1 agagatagga gtgtatcttt ttgggtgcag aattaaatca aaaattattt agtgcggcag
       61 ataatctccg tagtaaaatg gatgcgtcag aatataaaaa ttatttattg ggactgattt
      121 tctataaata tttatccgat cgattgttgg aacaagtggt tcttttagca gatgaatcct
      181 tagaggaata tgacacagta agcaaacaaa caatgttgta tcgtgaatta ctttcagatg
      241 aagaatcaaa agaagatctg attgccacaa tcgttgatat tttgggatat gcaattgcac
      301 cagagtatct tttcaacgtc ttagcggatc aagcaaaaca agcaacgttc caattgaacg
      361 atctaaacaa agcctttgtg caattggcat ctacctacaa tcaatttaat gggttatttg
      421 atgatgtgga tctccaatca aaaaaactag gaacagatga gcaacaacga aatgtgacga
      481 ttaccgaagt catcaagaaa ttgaatgatg tagatgtttt agaacacgat ggggacgtga
      541 tcggggatgc gtatgagttt ctgatcagtc aatttgcttc agaagcaggg aagaaagccg
      601 gagaatttta cacacctcat atggtttctg atatgatggc gcaaatcgta acgctcgatc
      661 aaaaggaacg acgattcttt agtgtttttg atccaacgat gggttcaggt tcattgatgt
      721 taaacgtacg taactactta acacacccag ataacgttaa atatcatggg caagaactaa
      781 atacaacgac atacaatcta gcaaaaatga acttgatttt acatggtgtc gacgccgaag
      841 aaatgaatct ccgcaatggg gatacgttga ataaggactg gccaacagat gaaccttaca
      901 catttgatgc agtagtaatg aacccacctt attcggctaa ttggtcagca gatacgactt
      961 tcttagatga ttctcggttt aatcgttatg ggaaattagc gccaaaatcc aaagcagatt
     1021 ttgcgtttct tcttcatggg ttctatcatt tgaaggaaac aggtaccatg gccatcgtct
     1081 taccacacgg agttttattc cgtggcgctg cagaaggggt catccgtcaa aaattactag
     1141 aagatggcag tatttatgct gtgatcggta tgcctgcgaa cttgttcttt gggacatcga
     1201 ttccaacgac ggtgattgtg ttgaagaaga atcgtcaaac tcgagatgta ttattcattg
     1261 atgctagtcg tgaatttgtt aaagggaaaa atcaaaataa actatcggaa gaaaacattc
     1321 aaaaaatcct cgaaacctac gctgaaagaa aagatgtcga gaaatacgct catttggcaa
     1381 cctttgatga aatcaaagaa aatgactata acttaaatat cccacgctat gtagatactt
     1441 tcgaagaaga agaaccaatt gatatggttc atgtcggaaa tgacatcaag aagatacgac
     1501 aagaacagca agtgctagaa aaagaacttt tagaagctat ctcttccctg caaacaacac
     1561 ctgaaaacga aatgtggtta caaggtgcgc tggaggtgtt caaacatgag caatgataca
     1621 cgaccagaaa tccgtttccc aggatttacg gaagattggg aagagcgtaa gttaattgat
     1681 ttagttgtta gacttaataa aagtacaaac tccagtagac ttccaaaagt tgaatttgaa
     1741 gatatcgtgg caggagaagg tagactgaat aaagatgtga gccagaaatt tgataataga
     1801 aaagggattg aatttttacc aaatgatatt ctatatggaa agttacgtcc atatttaaaa
     1861 aattggctca aggcaacttt tacaggcgta gcgattgggg atttctgggt ttttagagct
     1921 aagaagagcg actcggactt tatttattct cttattcaag ctgatagata tcaaaaggct
     1981 gcaaatgata catctggaac aaaaatgcct cgttcagact ggaaaaaagt ttcagataca
     2041 gtattctata ttcccaatga tttaaaagaa caacaaaaaa tcggcatatt cttcaaaaaa
     2101 ctcgacgaca ctatcgctct tcatcaacgt aagttagatt tgctgaagga aacgaaaaaa
     2161 ggattcttac aaaaaatgtt ccctgaaaac ggagcaaaag tgccagaaat ccggtttcct
     2221 ggttttacgg aagattggga agagcgtaag ttgggagaat taggtaaaac tcaatcggga
     2281 attggatttc ctgatgccga gcaaggtggt tctgaaggta ctccattttt taaagtttcc
     2341 gacatgaata acattggtaa tgagtatgaa atgagaaatg caaatcatta tgtatctaat
     2401 gagcaaattg agcgtaaaaa atggaagcca ataaaagatg tgccagcgat tatatttgct
     2461 aaagttggcg cagcaataat gcttaataga aaacgtttgg tcacaagccc tttcttaata
     2521 gataacaata ccatggctta tttatttaat aatacttggg atatctattt tggtaaaata
     2581 ttgtttgaaa ctataaattt gccaagatac tcccaagtag gggcattgcc ttcttataac
     2641 agttctgata ttgagaatat cagtgttaag gttcctgtaa aggatgaaca acaaaaaatc
     2701 ggtactttct tcaaacagct agatgatacg atcgctcttc atcaacgtaa gttagattta
     2761 ctaaaagaaa cgaaaaaaag cttcttacaa aagatgtttg tctaggaggg aagaagaatg
     2821 aaaattagtc accgtgagga agcagaagtc gaagaacagt tgattcgtgt acttggagaa
     2881 gggcataatc agtggacgta tcgtccggat ctaaaatcag aagaagacct ttgggtcaat
     2941 ttacgccaaa aaattatctc aaataatcaa gcagaactga atgattttcc tttgaccgat
     3001 aaagaatttg aaacgatcaa aacggaatta ctgctgcgaa cgaaaacccc atttgatgct
     3061 gcgaaatggt taaaaggtga aaatgggatg gctcggatca cgattgaacg ggaagattcg
     3121 caattgggtt ctgtctcact tatcctttat tccaatcaag acattggtgg aggaatctct
     3181 acttatgaag tggtccatca aattgcaaaa cgtggaagta acatagaagc acgggatcga
     3241 cgttttgacg tcaccttatt aatcaatgga ttaccgattg tccaaatcga gctgaaacaa
     3301 gtaactgcaa aagatggtgt ttatcaggca tttaaccaaa tcaaaaaata tgcagaagaa
     3361 ggtatgtttc ggaacaatat cttttcgacc ttacagttgt ttgtggtttc caatgaacaa
     3421 accactcggt attttgcaaa tgccttaccg aaagacttgc accctaagtt tttatttagt
     3481 tggcgaacga aagacaatga aaaagtagaa aacttatatg agttttgtaa acaagtgtta
     3541 aatatcccgg atgctcatcg tttgattgcg gattatacaa tcgtcagtga agatcaagat
     3601 aataaaaccc tcatggtgtt acatccttat caagtacatg cgattcaagc cttgtttatt
     3661 gccgcaaata aacatcaatc agggtatgtg tggcatgcga ctggttcagg aaagacgttg
     3721 actagttttg tatctactaa gcttttagca cgaaaatcgg ggatcgatcg aacgatcatg
     3781 ttagtggacc gcaaagattt agataatcaa accaccacag agtttacaaa atttgcttcg
     3841 gaatttaata ctgggatttc ttccgggaac gccaaagcga atagcttgat cgttggaacg
     3901 ggaagtgcca aagaattaag tgaaaccctg ttggcagatg ccaatgcaaa tgttgtaatc
     3961 attacaaccc gtcaaaaact agatgcggcg ttgaaatatg caaagaaaca agaagagaaa
     4021 aaaggaacca atcgcttcca aaaattaatg ggccaacata tcgtgtttgt agtagatgag
     4081 tgtcatcgag cgttaagtgc tgaaaatatg gaagaaatca aaaaaatgtt tccgaaatcc
     4141 acttggtttg gctttacagg tacaccgatt tttcctgaga atcggaaaca agccaaaggg
     4201 caattggcac gtacaaccca tgatcagtat ggagaggtct tgcataccta taccattaag
     4261 aatgcgctag aagatggctc tgtcttagga tttcaagtag agcatgaaaa caccgtggag
     4321 ccgacttcac tagagaataa aatctatcgg aaattaaaag aagtagaaac atatgcagag
     4381 tattcatcag aacagatcaa tcgtatgatt gatcaaatgg aacctgtcaa aaaagaaagt
     4441 tatttggacc cagctgtttt tgaagcagat gaacatatcc aaaaagtcat tcataaaatg
     4501 ttccgtccag ataatgccta tacaaaattt gattttcgaa atggcaggcc aacgaaatct
     4561 gcaatcttaa ccacgagttc cattgacatg gccaaaaagt attatcgggc catcaaagag
     4621 atgacgaaag agcctgattg gttaacaaaa gagtttccca atcagctcat tcgtgaagga
     4681 cgaacgatgg aggatcctga ttttccacga atcgctatta cctattcatt agaagaaaat
     4741 agccgagatt caagtgccca acaagaagaa atgcaaaaaa ttattgagga atataatcag
     4801 tattatgata cttcctggtc attagcagac attgagcgtt ataacggaga catcaataat
     4861 cgtttggcac gaaaaagagc ggaattcaaa caatttggga aacaaatcga tttagcgatt
     4921 gtagtggatc gtctattaac aggttttgac gcccctacga ttcaaaccct atttgtggat
     4981 cgtaatctag agtatgcagg attgatccaa gctttttcac gtacgaatcg tacgtatcct
     5041 gataagacaa aaggattgat tgtgacgttt cgtaagccag cgacaatgga gaagaatgtc
     5101 gaggatgcga caaaactgta ttcaaaagca aaagaagaat caggtttgat ttatccaagt
     5161 taccaagaat ccaaaaaaag attcaaacaa gcatatggta agttaaaaga ggtttcatcc
     5221 atggaatcca ttgatgaaca tacgccactt gaaatccgta tcgagtacgt caaagcattt
     5281 caagaattga atcgtgctta tgaatcgtta gtgacttatg acgattacaa tgatgacatg
     5341 gtgaaatctc caactttgaa caatcaaatt caacttttgg aagaacaaat tggcgtgtac
     5401 gagaccgtga agggttcatt aatagaggaa gaaccagaaa aagaaggaga agatttttca
     5461 acgatcgaat tttatagtga aaataccact aagttgtatg atattgattc aacttatatt
     5521 gatcaattac taggaactta tgctgcgaat agttcagatg cacgagaaga aattgaaaaa
     5581 gctttggcta aattgaataa aacagaagga gtcaaagaag tttaccgaca aattttaaat
     5641 gcatttgatg tggggacttt agatagtaat gaagatatct ttgtcattaa acgtcgctat
     5701 tttacacaag cgagtgatga tttgattcat gtcttttcca atgagtggtt tgtttctgag
     5761 aaagaattac atgcttctaa tctgcaatat ctaccaggtg aagatccaat tcctaatatg
     5821 aaagcaatta tcaacagtaa ggattacgaa ggatataaag ccaaacatcc agaagcgaag
     5881 ccatttaaat acccacaaga aatgaagcga gcatggcgta aaatgttaga tgatgagttg
     5941 attccacttg agaatgaatt gaggtaaacg agaagagtta agaaaattgt acgattcaaa
     6001 gatggtagtc aagtggaagt gtagaaaata gggatagatg tcttttattt gttcacattt
     6061 tataacgaag tatttaagac agctattgaa taatgtttcc tccacctttt tcattcattt
     6121 accccacccc gttgttatat gaccgtgttt tgagaggaac caataaaaag cataggctaa
     6181 ctaggggttc ctttttcttt tgtttttttg gaatccgctt aagtgaaatg tatctgaaat
     6241 gaaacggaga taaagtgatt ttttgctata atgacaaggt tttagctgtt tcagatacca
     6301 tgtaacccaa aatagtttat tgagaaaata ggagagtatt tgatactaac tagtaggaaa
     6361 aaatgagata aaaattaaat ggaatcattt caggtaatct caaataaaaa aatatacagt
     6421 cctgtggcac tttacctatg ggactgtata ttttttactc tcagcgggct tgttttgact
     6481 ccgtgtctga ctccgtcgta ttgattttaa atgatattcg atgaccattt aacaggtcat
     6541 ttttgaaatt tttgtctcta tatgactcag tttgataatt attgatagat caatttgggt
     6601 ctatatcgaa caagggaata atccaataaa aaatgattta gaatatcaaa ataacaaaaa
     6661 agaggactac ctgtattcgt cctctttttt gaccagattt tgaccagtct aatttacatt
     6721 ctaatatatt catatgagct aattttttga ttaaatggta agtttttcct tgtttgtttt
     6781 agtttaaact agtgtgtaat ttaaaaatct atatggaagg gtctgtgggg taatacttaa
     6841 ataaaggcta ggaacctgat aacatcaagg ttcctagcct ttttataata aaatgaagcg
     6901 aaaataattt cttttaaaga gccgagatta tcagctatat aagagtgaat ttgatgcaaa
     6961 aaacagagcc gaaacagtct tggtgttcgt agctctgctt atatgtttcg aatttaagac
     7021 aaagagttgg caaaagatgt tgataaggaa gataagataa ctgtgttata taaactaggt
     7081 aactaactgt aacaagctaa ttttaagaga ttcttattag aagacgttta ggaatataac
     7141 taagaaaatg attatacagg taattgtcat ttcaataatg ttttctaatt ttcgcgtctt
     7201 tgttttaaga aaaaggccag aatcagttgg aaaaaagttc gtacgatagt gaagaggggc
     7261 aaaatttcct actaatattc ccactaacaa gagataaaac ggccaggttg gtgtgattcc
     7321 tctataaaaa aatgcccatg ctaccgaaat cccatagatt aacaactgaa caatttttac
     7381 aatgaaaaaa ttgttctgcc attactcaaa gtctcttcct ccttgtatcg tttatttgtt
     7441 tcattttatc attatgatag gcgcttgaac aatccatttt gtattttatt gagtactctt
     7501 ggataaacta ccagcgatta tcatatttat ttatgttact gtaagccatc atataaaagt
     7561 aaatgagcag atcatagaaa tataaaaaag aactaaaatt gatctaaatt atgtgcagct
     7621 tgactacgta ccaatctata ttacttacta gattggtcgt taatctattc ggatcgaatg
     7681 agcaaaatat ttttgtgaat gtccatcctt gattatgatt ccaaaaagaa tcattgggag
     7741 gctgtggcgt aaatattata ctataagcca gaaataccta tcattctgaa cttttttatt
     7801 taggaacttt catctcaagg ctttttcaat taatttgtac ccctgttttt tggataagat
     7861 catgtgcatt gattttttct atctctacgt tcatctccta cttaatgcct atctggctcc
     7921 tgagggcttc tttttaaggc gtcgtcatag tcttggctga ggatttcttg gtaatctgag
     7981 ggaatggctt cccaagtgag caggcgaacg ttcatgacgt gttttccttt gtgtttgatt
     8041 acagcatggt ttttctcttt ttctacctct ccagcgtgaa aagacagcca tgtaccatgg
     8101 ccaaagatat cattacatac ccaacgttcg atggaattgt ccacgtatcc tacagggagt
     8161 gtggtatagc cgatttgtgg tttcaaaaaa ggattgaatc gatcgatcgt ttcatttttg
     8221 tattgataat tgataagaaa aagatagacc aaacaaaaga cgagcagaaa gaaagatagt
     8281 gtctttttga gtgagaaggg ttgttttgat gaataagatt tcttgttaac aagaggttca
     8341 tctggcaatg ttggttctat tgattcggta gctgattcaa tagaaggaaa atcaatcggc
     8401 agacctaaag tatgatgtaa aagtgacagc atttcaaagt ctggcattgt acgagaattt
     8461 tcccaactag aaatcgtttt atcggaaaca ccaatttttt cgcctaattc tttttgcgta
     8521 agcttacaat ttctgcggta agttcgtatt ttttctccgg tgttcatctt cttctccttt
     8581 cgattctaca gtatatagat ttattctaca tgacctatac acttgataga tacgtttttt
     8641 tgatgaaaaa cggtatgatt ttatcaaaga atcatttaag taagggagag tggtgataat
     8701 caaaaaaaag ttcacagttt tactcacaat catagtgacc ggtatcatga gtggttttgc
     8761 cttggttagt ataaataaac aggtactcta tcggacaaat gaatttttcc atcaaacaga
     8821 tacttatact ccgattcgat tgccacaaca ttttattggg caagaagaat atgagcagct
     8881 aatcatggta tttgaaaatg tctcatcaga aactgagctt tcttatatga aaaaaacagt
     8941 aaatgaatcc aatgaagtag atacatcttg gaacttcaag atacagccaa agcttgaaat
     9001 gacattgtat tatcaagggg atgtcaaatc ttctcacgag aaggtaccct attctaatac
     9061 ctcctttcgc gtaacgtcca ttgaagaatc gatcaaaata aaagattttg aaggtgaatt
     9121 tttcttgatg acgagagaaa aagaagccta tgaacgggtc atttcactat tcactgatcg
     9181 attcaatcaa gcatttcaca ctgattatgc cactaatatt tttgaagact ttgaaaatag
     9241 ctattcgtat tcggttattt caaatacaga cgtatataat tatcgagtgt atttaactat
     9301 tggattgat
//
LOCUS       DAXWLF010000107         9199 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXWLF010000107 DAXWLF010000000
VERSION     DAXWLF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.107"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(139..402)
                     /locus_tag="ACGE4U_001921"
     CDS             complement(139..402)
                     /locus_tag="ACGE4U_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFF8394051.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(501..1796)
                     /gene="rho"
                     /locus_tag="ACGE4U_001922"
     CDS             complement(501..1796)
                     /gene="rho"
                     /locus_tag="ACGE4U_001922"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFF8394052.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(1793..3064)
                     /locus_tag="ACGE4U_001923"
     CDS             complement(1793..3064)
                     /locus_tag="ACGE4U_001923"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFF8394053.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(3229..4098)
                     /locus_tag="ACGE4U_001924"
     CDS             complement(3229..4098)
                     /locus_tag="ACGE4U_001924"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFF8394054.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            4201..4413
                     /locus_tag="ACGE4U_001925"
     CDS             4201..4413
                     /locus_tag="ACGE4U_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394055.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(4390..6000)
                     /locus_tag="ACGE4U_001926"
     CDS             complement(4390..6000)
                     /locus_tag="ACGE4U_001926"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFF8394056.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(6222..6467)
                     /locus_tag="ACGE4U_001927"
     CDS             complement(6222..6467)
                     /locus_tag="ACGE4U_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394057.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(7193..8533)
                     /gene="glnA"
                     /locus_tag="ACGE4U_001928"
     CDS             complement(7193..8533)
                     /gene="glnA"
                     /locus_tag="ACGE4U_001928"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFF8394058.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(8561..8953)
                     /locus_tag="ACGE4U_001929"
     CDS             complement(8561..8953)
                     /locus_tag="ACGE4U_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8394059.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         2587 a         1980 c         1578 g         3054 t
ORIGIN      
        1 ctagtttttc attgttttgt gcattatgat cctacgactt tttcagcatc aaacgaagtt
       61 ttaaattggc aaaaaacata aaaaaacaga ctatccctat cagatagtct gctaaacgtt
      121 gagttatcag cgtttttatt attccgcagc gtttgcgtct ttgagaccgt attttttgtt
      181 gaaacggtcc acacgtccgt ctgcttgtgt gaatttttga cgtccagtgt agaatggatg
      241 tgagtcagaa gttacttcga cacggatgac tgggtatgta ttaccgtctt cccattcaac
      301 tgtttcttgt gaagtttttg ttgaacctga caagaatttg aaaccagttg ttgaatccat
      361 gaatactact gggtggtaat ctggatggat gttttctttc atgatttttt cgctcctttg
      421 ccctgctcca tttgctgaac cagagttgat ttgtcgattt acaacattaa catcttaaca
      481 tgtttcagtc aaggttgcaa ttatcttttc agatttctgg tcggattttt tttaccaaag
      541 gagacatctt gaaattcttt gaaaaattgc tgattgtttt ttgttttacg caaaaagcgg
      601 atcagttgtt ccgtatattc taatgaatct ccagtcatat tttttcgaag tttccaaatt
      661 tcttctaatt cttcacttgg catcaataat tcttcttttc gggtacctga ttttttgata
      721 tcgatggctg ggaagatccg acgttcagat aattcacgtg aaagttgtaa ttctagattt
      781 ccagttcctt tgaattcttc atagatcacg tcatccatac ggcttcctgt atcgactaaa
      841 gcggtcgcta aaatcgtcaa actgccacct tcttcgatgt ttcttgctgc cccgaaaaat
      901 ttcttaggtt tatacaatgc ggctgggtca atcccgccac tcaatgtacg tccgcttggt
      961 ggaacgacta agttataggc ccgagcaagt ctagtaatac tatccatcaa gataacgaca
     1021 tctcgtttat cttcgactaa acgcattgcg cgttccaata ctaattcaga aactcgagta
     1081 tggttttgcg gctgcagatc aaatgtagaa gataccacgt cgcctttcac gcttcgttct
     1141 agatccgtta cttcttccgg tcgttcatca atcagtagaa cgattaattc tacttccgga
     1201 tagttttcgg tgataccatt tgcgatttct tttaatacag aagttttccc tgcttttggc
     1261 ggtgcgacga tcaatccacg ctgaccaaaa ccgattggag aaaagatatc gatcattcgg
     1321 gtagatagtc tttgcggtgt tgtttccagt tgaagctgtc tttgcggata aagaggtgtt
     1381 aaggctggaa aatgcggacg ttcttttgct tcttccggat cttttccatt aacgctttcc
     1441 acgtgcatca aaccataata acgttccgat tctttaggtg gacgggcttt tcctgccact
     1501 ttgtccccat ttctcaaact gaatcgtctg atttgtgagg cagaaatata gatatcttct
     1561 gcacttggtc cataattgat tggccgtaaa aaaccgtaac catcttgacc aacgatatcc
     1621 aatattcctt ccatataata aaatccttgt ttctctgctt gtgcacgaat cacagctaga
     1681 gataattcct ttttattcat cttactgtaa taaggaattt taaattcttt ggcataacta
     1741 taaacttctt ttaacgtttt gttctctagt tctgccattg tcaaataatc gctcattcag
     1801 ctcccacttc ctctatcatt tcaattgatg cccctaaggc tgttagtttc tcgataatat
     1861 gatcataacc gcgtaagata taatctacat tgtaaatcgt cgttgtccct tctgccaata
     1921 gtccagcaat cactaaacaa gcacctgccc gaagatcaga agccacgact tctgtgccgt
     1981 gaagtttatt tggaccattc acgatgatca tatttccttc cacggtaata tcagcgccca
     2041 ttcgtgctag ttccggcaca tggttcacac gtttcgcata gatcgtatcg atgatttctg
     2101 aagtgcctgt agtcatcaat agtaacgcag taagtggctg ctgcagatct gtggcaaatc
     2161 ctggataagg ataggtcatg atgtttgcgg gttttaattc tttcgagggg taaacttcga
     2221 tttcatcttc gctgattttc atatggacac caatttcctg caattttgct ataaagcttt
     2281 ccaaatgttc aaagatcaca tttttcactt tgataccatt tcctactgca gctgctaatg
     2341 ctagataggt tcctgcttcg atgcggtcag gaatcatgaa atgccgacag ccatgtaatg
     2401 tttcgactcc ttcgatacgg atcacatctg tacccgctcc tcgaactttt gcccccatat
     2461 tattcagtaa agttgccaca tctatgattt ccggttctcg tgcagcgttt tcaattactg
     2521 tctgtccttt tgcttttaca gcagctaaca tcacattgat cgttgctccg actgaaacca
     2581 cgtccatgaa aatccgattg ccacgcaatc ctttattttc tgtccgtaaa tacatggcac
     2641 catgttcatt tgtcacttca gcacctagag cttcaaatcc tttgacgtgt aggtcaatcg
     2701 gtctagggcc taaataacag ccacctggca ggcctactac cgcttcgcca aatttaccaa
     2761 gcagtgttcc cataaaataa taagaagcac gcaaactatt gatttttccg tgcggcatcg
     2821 gaatcgatac gacttctcga ggatcgattt ctagtatgtt gttggaaaaa tggacagttg
     2881 cgcccatgat ctctaaaatt tcaattaatg aatgaacatc ttgtatgtcc ggcacacctt
     2941 ccaagatcac tggggaatca gctaagattg cagctggaat caatgcgact gcactatttt
     3001 ttgcaccgct gatcgttact tcaccagcta aaggtcggtt tcctttgatt acgatttttt
     3061 tcatctgttt ccaccttgtt tattaatctt ttttcatgga tatgcttgta ttttatcata
     3121 gataaaaaaa agaaacgagc atggtctgcc gttttaagta aaaaagaatg gagagcgact
     3181 tcaaaagtcg ttctccattc ctatatatcg tatctgttat gtggatgctt acgctttgtt
     3241 agctgaaccg aaccagtcga tatgagattt agcatcttca acgattgctg ctttacctgg
     3301 agctaataat ttacgagggt cgaatccttt accttcttta tctttaccag cttcgatata
     3361 tttacgagta gctgcagcaa atgataattg gaattcagtg ttaacattta cttttgagat
     3421 acccattgag atagcttttt cgatttgatc ttgaggaata cctgatccgc catgtaatac
     3481 taaaggcatg tctggaccta cagcatcagc aattgcttgt aagtggtcaa aagctaatcc
     3541 tttccagttt tctggatatt gaccatggat gttaccgata ccgcaagcta agtagtcgat
     3601 acctgtagca accatttgtt tgcattcttc aatatcagct aattcgccgt taccgatgat
     3661 tccgtcttct tctccgccga ttgaaccaac ttcacattca actgagatac cttttgcatg
     3721 tgctttttca acaacgtctt tagctaattt caagttttct tcaaatggta gatgtgaacc
     3781 atcgaacatt actgaagtat agcctaattc gatacattcc aaagcagctt cgtaatcacc
     3841 atggtctaag tggatagcaa ctggaacagt gatgttcatt gcttcaacta aatcagtgat
     3901 taaatcttta cagattttgt aaccacccat gtatttagct gcacccattg atgtttggat
     3961 aagtactggt gcttgttttg cttcagccgc ttcaaggatt gcttgagtcc attccaagtt
     4021 gtttgtgttg aatccgccga cagcgtaacc gcctttacga gcagctttta aaaattcagc
     4081 tcctgatact actggcataa taaaattcct cctaagtttt gtaaaactaa atttgttaag
     4141 acaattctta accaacgacc ctattttacc aaacatttcg gtagttttct actaaaatca
     4201 atgaaaatga aaattttcaa gaagtttttt gcaaaaaata aaatatccaa tagcgcttta
     4261 atcgaaaaaa gtcttttgct ttctatcttt tttcctacaa ataataacaa atcagaaaaa
     4321 gagaaaagtg acaagaaaat tgcttatccc cttctcttgc tacttttctc tttctatcaa
     4381 gttcaaaaat tattttgttt cgtgatctgc taatgatgct ccgacaaatg ctttgattaa
     4441 tttttgcggg cgatttggac gagaaatcaa ttctgggtgg aattggcatc ccacaaagaa
     4501 ttttttctct ggcagctcta cgatttctac taaacggtta tctggtgaga cacctgaaaa
     4561 gactagccct tgttcttcaa acaattgacg atatttgtta ttgaactcat agcggtgacg
     4621 gtgacgttct tgcacaacat cggctccatc atatgcttca gccgtcttcg ttcctttttt
     4681 gattttacat gggtataatc caaggcgtaa agttccaccc aaattctcga tgttttcctg
     4741 atcagccatt aaatcaataa tattgtttgc tgtatctgga ttcgtttcag ctgatgacgc
     4801 atcttctaat ccaacgacgt tacgtgcaaa ttcgacacaa gccatctgca taccaagaca
     4861 aatacctaaa aacggaacgt cattttcgcg agcataacga atcgcttcga ttttcccttc
     4921 tactccgcga tcaccaaatc caccaggaac caagattcca tctgcgtctt tcaaacgttc
     4981 ttctacattt tctctcgtta attcttgtgc tttgatccaa tcgatctcga tgtctgaatt
     5041 ataggcgaaa ccagaatgtt tcaatgcttc aactactgag atatatgcat ctggtaactc
     5101 gacatattta ccgacaagcg caatccgtac ttttttcttc aagttcaaga cacgttcttc
     5161 taattctttc cattctgtca tgtctgcttc tggtgtatct agttttaaat ggtcgcaaac
     5221 aatttgatcc attccttgtg cttgtaaaac taatgggatg gagtaaagtg tatctacatc
     5281 tggcgactcg ataactgctt ctggttcgac atcacaaaat tgtgccaatt tgtttttcac
     5341 gctttgagaa acaggttgct ctgtacgtac caccaaaata ttcggttgga tacctaaacc
     5401 acgtagctct tttacactgt gctgcgttgg tttagttttc atttctccag cagctttcaa
     5461 gtaaggaatc aatgttgtat ggatatacat aacattatct gtaccgacat ctgctttcat
     5521 ttgacgaagt gcttcaagga atggcaaaga ttctatatcc cctactgttc cccctacttc
     5581 tgtaatgatc acatctgaat cagtcatttt agctgctcgc atgatttttt ctttgatctc
     5641 atttgtaata tgaggaataa cttgaacagt tgctcccaag tattcgccct tacgttcttt
     5701 acgcaaaact tcagaataga tttttcctgt cgtcacgttg gagtatttgt tcaaattgat
     5761 gtcaataaaa cgttcgtagt ggcccagatc caaatctgtt tcagcaccat catctgttac
     5821 gaatacttct ccgtgctgat atgggctcat cgtccccgga tccacgttga tgtatggatc
     5881 aaatttttgg atagttactt ttaagccgcg gtttttcaat aagcgtccta aagaagctgc
     5941 aacaatccct tttccgattg atgaaacgac accgccggtc acaaaaatat attttgtcat
     6001 aagttcaaaa actccttttt aatttatgtg caggaaaagt ctcagacaat aaacaaagct
     6061 ccctattctt caataagaat agggagccga aatttctgta aacttctgac cctctaaaag
     6121 ggtgcccaag gtgtataata caagctgact aagggaaagt caagtactca aatgaatact
     6181 atccgcttac aattttttct ttttcgatat cctttgagaa attatttctt aagtccttta
     6241 tgagtgcaaa aaaaatttaa cccacataat aagcttgata taaacatagc taacgttaaa
     6301 cctaagcaaa tcagaaaatc gttactgaaa aattttaagt gaaataatag ataaaaaaat
     6361 aaaacaaaca ttatgataga taatatccca cctaacaaag aactactttt tcctacttgc
     6421 agcaacagtt tatatagctt atcgtagaaa caatcttttt ctttcaccct tatttacctc
     6481 ctttacgtaa aaaacgtagt ccacagttct cggatttttg gcatatcttt tctattaaat
     6541 aatatcaaaa acaaaacaat tccactaatt ttttcaaaga tgaccttttt ttcaacaaaa
     6601 gtgccttgta gggattcatt gaaggtaacg aatcacataa gtaaaaaata aggagatgcg
     6661 acaaaagtcg tgcctcctta tttctaaata aacggtgttc tcctgccaac tcctcggtca
     6721 caagtcacaa tatccatcaa acatgagaag ctgtttttga aattatttct tgtgattcgg
     6781 agttaaatgg taggctcaca acctctggat aaacggtatt caaaagccag tttctcggta
     6841 ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt cttaatactc
     6901 gaaactaaac gtctttttca aaacctcttg gtaaacggtg ttcaaaagct tgcatctgcg
     6961 gtaataagtt cagccaattc aaaaacaaga aaagctgttt ttgcttggct attcttatta
     7021 cttgttgcag ggctgctttt tcacaacctc tccactaaac ggtgttcaat cagctgaccc
     7081 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat ttttccgtta gtttctctaa
     7141 tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg ttttaataaa
     7201 gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc gcccattcga
     7261 gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca atcattacat
     7321 tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc tggatatgtg
     7381 cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct gctggcggtg
     7441 tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt gccaagtatg
     7501 ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg cgagattctg
     7561 gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca ggcgcttcat
     7621 aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct gtgtaggcac
     7681 gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt tccaattcac
     7741 cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg caatgcatac
     7801 cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt ccatgcttgc
     7861 gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca tctaccacat
     7921 ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt gaagcttcca
     7981 cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt tcacctaaat
     8041 ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg atcccaccgt
     8101 cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa gaggtaaaac
     8161 caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg ccggcaaatg
     8221 gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca tgagcacttt
     8281 cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg ctttcttcaa
     8341 ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct aatactttgt
     8401 cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct gtgaacatca
     8461 atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca cctgttatct
     8521 gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag aattgtcttt
     8581 gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc aaaatctcat
     8641 cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt gcttcttgtt
     8701 ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat ccctctgaaa
     8761 gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata cgtcggtttc
     8821 cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt tgccgagcag
     8881 acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat cggcgcaatt
     8941 ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg taccaattaa
     9001 aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta ttttttataa
     9061 aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac cagatacctt
     9121 ttatttttta ttatactgta ctatccttta ttagtgtttt tattgcgccg tttgtataat
     9181 taagcgagac aaataaaaa
//
LOCUS       DAXWLF010000108         9001 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXWLF010000108 DAXWLF010000000
VERSION     DAXWLF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.108"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(69..1208)
                     /locus_tag="ACGE4U_001930"
     CDS             complement(69..1208)
                     /locus_tag="ACGE4U_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFF8394060.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1607..2905)
                     /gene="eno"
                     /locus_tag="ACGE4U_001931"
     CDS             complement(1607..2905)
                     /gene="eno"
                     /locus_tag="ACGE4U_001931"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFF8394061.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(3021..3776)
                     /gene="tpiA"
                     /locus_tag="ACGE4U_001932"
     CDS             complement(3021..3776)
                     /gene="tpiA"
                     /locus_tag="ACGE4U_001932"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFF8394062.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3880..5070)
                     /gene="pgk"
                     /locus_tag="ACGE4U_001933"
     CDS             complement(3880..5070)
                     /gene="pgk"
                     /locus_tag="ACGE4U_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFF8394063.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5180..6181)
                     /gene="gap"
                     /locus_tag="ACGE4U_001934"
     CDS             complement(5180..6181)
                     /gene="gap"
                     /locus_tag="ACGE4U_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFF8394064.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6222..7313)
                     /locus_tag="ACGE4U_001935"
     CDS             complement(6222..7313)
                     /locus_tag="ACGE4U_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFF8394065.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7462..8796)
                     /gene="rpoN"
                     /locus_tag="ACGE4U_001936"
     CDS             complement(7462..8796)
                     /gene="rpoN"
                     /locus_tag="ACGE4U_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFF8394066.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8856..8927
                     /locus_tag="ACGE4U_001937"
     tRNA            8856..8927
                     /locus_tag="ACGE4U_001937"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8889..8891,aa:Arg,seq:ccg)
BASE COUNT         2336 a         1905 c         1658 g         3102 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgtttatta
       61 aaaaaatctt acgctaagta ggataaaata attccttgat atttttcgat catatccaaa
      121 taattatcga tttcaacata ctcatctact tgatgaggca aagtaacgac accaggccca
      181 aagacgacaa agtcgaagga atggttagct ttcgtaaatt cggctgcatc cgttgttgct
      241 gccgctccaa ctaaaggtaa tggctggctg aattgttgct gaatgcaatg aatcaaagga
      301 gaatctggat cagctttgac aggtattttg ttgtaatcga tcatcaattc caaatgatag
      361 tctgtctctt gattcaattc attgacgatt gattgcagca atgcaatgat tttatcattt
      421 gggtattctg gaatcgatcg aatgttccct tggagctgcg catggcttgg gatgctgttg
      481 acttgattcc cgccactgat caatgtaaca ttatggattg tttttcctag gacaggattc
      541 tcgattgttt cagcgaggtg attcatttca gcatttgctt tcgtaatgaa ttcatttagg
      601 tgattgattg cattataacc ttggtctggc atagagctat gcgcttcttt tccgtgcgag
      661 gtgactgtat agttgattga tcccatgtgc gtatacatca aactgtaatt ggttggctct
      721 ccgatgatca aggcatctaa gtcatctaca tagcctgctt tcgtgagctg ttccccacct
      781 agttcaccca cttcttctcc gacagtggct aataatttga ctgttccgtt aaatggtttt
      841 cctgactctt tcagttcgat catggcaatc accattgctg ccaagccact cttcatatct
      901 gtagcaccac gtccgtacaa acggttccca tggatttctg ctgcaaaggg tgcataggtc
      961 caagacgatt catcaccagc cgccacgaca tccatatgcc cagataggcc taacactttt
     1021 cctgattggc ccttttggta acgagcaatc aaattatctc taccatctct gtaagaaaca
     1081 atctctcctg ttatgtcatg ctttgccaaa agcttattca aataggctgc tacttcacct
     1141 tcgtttccat tcactgattt aattcggata atctcttgta aaatcgcgat tttttcttct
     1201 tttttcatct aatcctctcc cttgccattt ctttttattg tatttgagtg tgctcgaatt
     1261 ttcaaatagg agcaaccaaa gtaaaaatga agaaagagag ccactttatg aaaaatatgc
     1321 tgctacttcc ctcctatttt atttatacga gaggacttgc ccatagattc ctattctact
     1381 gttctatcct taaaagctct tatatgatgc tagttattgt tagctttcct ttttcaagga
     1441 tgatcttttc ttgtaccatt ctttgtaaac atcatataca gaagctagtt gcaaaaaaac
     1501 tctctacctg ctgatatttc agcaagtaga gagttcgatt acttaactaa gtgatcatac
     1561 tcgactatct ttcaatagtc gatcatgtat cttcattaga aaagaattat ttgtttttta
     1621 agttgtagaa agatttcaaa cctttgtatt ctgcaacttc accaagttgg tcttcgatac
     1681 gtagtaattg gttgtattta gcaatacggt cagtacgtga tagagaacca gttttgattt
     1741 ggccagcgtt tgttgcaaca gcgatatcag agattgttga atcttctgtt tcaccagaac
     1801 ggtgagatac aactgctgtg tagccagctt ctttagccat ttcgatagct tcgaaagttt
     1861 ctgttaatgt accgatttgg ttcactttga taaggattga gttagcaatt cctttttcga
     1921 taccttcaga taatttttga gtgtttgtta cgaacaagtc gtcaccaact aattgaactt
     1981 tgtcgcctaa tacgtcagtt aatttcttga atccgtccca gtcgttttcg tctaatccat
     2041 cttcgattga gatgattgga tatttagaaa ctaattcttc gtagaatttg atcatttcgt
     2101 cagttgtttt ttcgccttcg cctgagtcag ctagtacgta aacacctttt tctttgtcgt
     2161 agaattctga agaagcagca tccatagcaa gaacaacgtc tttaccaggt acatagccag
     2221 ctttttcgat tgcttcgatg attacttcaa aaccttcttc gtttgaacca aggtttggag
     2281 caaatccacc ttcgtcacca acagaagttg ctaatccgcg agctttcaag attgaagcta
     2341 atgcgtggaa tacttcagca cccatacgta gagcttcttt gaatgttgga gcgccaacag
     2401 gcatgatcat gaattcttgg aagtcgattg agttatcagc atgtgatccg ccgttgatga
     2461 tgttcatcat tggtgttggc aatacttttg tgttgaatcc gcctaggtag tggtataaag
     2521 gtacttctag gtagtcagca gctgcacgtg caacagcgat agaaacacct aaaatagcgt
     2581 tcgcacctaa tttacctttg ttaggagttc cgtctaaagc gatcatagct ttgtcgatag
     2641 ccatttggtc acgaacatcg tagccaatga ttgcttcagc aatgatgttg tttacgttgt
     2701 cgacagcttt agttacacct ttaccgccat aacgagcttt gtctccgtca cgtaattcaa
     2761 ctgcttcgta ttcaccagtt gaagcacctg aaggaaccat tccgcggcca aaagctccgc
     2821 tttctgtgta tacttctact tcgattgttg ggttaccacg tgagtctaag atctcgcgag
     2881 cgtaaacatc agtaataatt gacatgtttt gtctctcctt tgagtttgtt ttttcctaag
     2941 ggatttccct catatacgat tttagttaat tttgctcatt agtgcaaaca tttcttgcat
     3001 ttttcgcaaa ttttcaacaa ttattttaca gcgtctaaca atgacaagaa tgaatctgct
     3061 tgcaagcttg cgccaccgac taaagcaccg tcaacatttt cttttgccat gtattcagca
     3121 atattttctg gttttacaga accgccgtat tggatacgaa ctgcttctga tacttctttt
     3181 ccataaagtt tttcaactgt ttgacgtaca actccacaaa tttcgtcagc gatttgtgcg
     3241 tcagctgatt taccagtacc gattgcccag attggttcat aagcaataac catgtttgac
     3301 acctgttcag ctgaaagatc agctaaacca gctgtaattt gtccttcgat ccattcagca
     3361 gttttgcctg cttcataggt ttctaaagat tcgccgcagc ataggattgg tgtcatacca
     3421 tttgcaaaga tagctttcgc ttttttattg atgtcttcgt ctgtttcatg gaaatattca
     3481 cgacgttctg aatgtccgat gatcacataa tgaacaccta gatcagcaag agctgctggt
     3541 gaagtttcac cagtgaacgc acctgagttt tcccagtagc agttttgtgc tgagattttt
     3601 agttctgtac cttcagctga tgtcattaat tcttgtaaga ataatgcagg agatccgatg
     3661 actgaatcaa ctttgtcatt tgaagggatt ttgtttttta ctgcttcagc aaattctttt
     3721 gcttctgatg cagttttgtt cattttccag ttacctgcga taataggttt acgcattgag
     3781 gaacacactc cttaatttat tgaaaaatgg ctagggagtc atgaaaactc ccttcacctt
     3841 ttgttcgatt actcgtcaga attgagcaaa cttggtcgtt tatttatcgt tgattgctgc
     3901 tagtcctggt aattctttac cttcaagcaa ttccaagctt gcgccgccac ctgttgagat
     3961 gtgtgtgaat ttgtcagcaa atcctaattg ttctgctgct gcggctgagt ctccgccacc
     4021 gatgattgtt gttgcgcctt ctaagttagc gattgcttca caaacaccga ttgtaccttt
     4081 agcaaagtta ctcatttcaa atacacccat aggtccgttc caaacaactg tttttgcacc
     4141 ttgtaatgta tcagcaaata attttactga tgctgggccg atgtctaaag ccatcaaacc
     4201 atcaggaatg tctccttcga ttacttgtgt ttcaacatca tttgaaaatt ctggtgcaca
     4261 gacattgtcg attggcaaga caagtttgtc gcctgctttt tcgattaatt cttttgctag
     4321 ttccacttta tcagcttcta ctagagagtt accgatcttg attccttttg cttcgtagaa
     4381 tgtgtaagtc attccgccac cgatcaagat tttatctgct ttaggaatca agttttcgat
     4441 tacgccgatt ttatcagaaa cttttgcgcc accaaggatt gcaatcattg gtcgttttgg
     4501 ttcttctact gcttcaccga tgaacttgat ttctttttcc atcaagaatc cagcaactgt
     4561 tgggatacca gttgaagcga tacctacgtt agaagcatgt gcacggtgag ctgtaccgaa
     4621 agcatcgtta acaaatacat cgcctaatga agcccagtat ttacctagtt ctgtatcatt
     4681 tttgctttct tttttaccgt caatatcttc aaatcgtgta ttttcgaata cgacaacgtc
     4741 gccgtcattc atgttgcgga ttgcttcttc taattgttct ccgcgagttt caggtacgaa
     4801 tgttacttct ttacctaata attcgcctag acgttttgca actggagcta aagattttcc
     4861 ttctttatct tcttctgttt ttacacgtcc aaggtgagag aaaaggattg cttttccgcc
     4921 ttgttctaga acgtagttga ttgttggcaa tgctgctttg atacgagtgt cgtcagtgat
     4981 cacgccgtct ttcaaaggaa cgttgaagtc aacacggaca agaacttttt tgtcttttaa
     5041 atcgatatct ttcactgttt tcttagccat tttatttcct cctgaataaa cttattatca
     5101 aaaaaagcgg aagaagcgcg attgcttccc cgcttttaaa tgtacatgtc tataatttac
     5161 acttgtacgt ttgtctatct tataagttag cgaagtattc taaagtacgt actaattgtg
     5221 cagtgtatga catttcgttg tcgtaccaag caacagtttt aactaattgt ttgtcgccaa
     5281 ctgtcattac tttagtttgt gtagcatcaa ataatgaacc gtaagtcata ccaacgatat
     5341 ctgaagaaac gatttcgtct gtgttgtatc cgtaagattc gtttgaagct tctttcattg
     5401 ctgcattgat ttcgtcaaca gttacttctt tttcaagaac tgttactaat tctgttaatg
     5461 aacctgttgg tacaggaaca cgttgagcag ctccgtctaa tttaccgttc aattcgggaa
     5521 ttactaaacc gatagcttta gcagcacctg ttgagttagg aacgatgttt gcagcagcag
     5581 cgcgtgcacg gcggaagtca cctttaggat gaggtccgtc tagagtcatt tggtcacctg
     5641 tgtaagcgtg gatagttgtc attaatcctt caacaacacc aaatttgtcg ttcaatgttt
     5701 tagccatagg agctaaacag ttagtagtac atgaagcacc tgagataact gtttctttac
     5761 cagttaatgt ttcgtggttt gtgttataaa cgattgttgg aacatcgttt ccgccaggag
     5821 cagagataac aacgcgttta gcaccagctg ttaaatgttt ttcagctgct gatttagaag
     5881 tgaagaaacc agtacattct agaacgatat ctacgcctag ttcgccccat ggtaattctt
     5941 ctgggttgcg gttagctaat actttaactt cttttccgtt tacgttgaat gaaccttcat
     6001 gaacttcaac tgttccgttg aaacgtcctt gagttgtgtc gtattttaac aagtgtgcca
     6061 acattttagc atctgtcaag tcgttgattg ctactacttc gattccatct acatcttgga
     6121 tacgacggaa tgctagacgt ccaatacgtc caaaaccatt aatacctact ttaactgtca
     6181 tttaagattt cctccttatg aaaatcaaaa aaatatttat tttaaagggt tacccctttt
     6241 aaaatctcat ttgcagcagc ttcatcagtg atgagccacg tttgttttgg tgcatttttc
     6301 atataagctc gaattgcttt agcttttgat tttccgcccg cgattgccat gatgatcggg
     6361 actttttcca aatcttgcaa ttctatgccg attctaggga ccttataaac gacttcacct
     6421 ttttcattga aaaagtagcc aaaagattcc gcaacagcat ttgcttgttt cagcatgact
     6481 aattcttttt cagtcatctt acgacgtgct gccatgtgta atgcacgccc aatactatga
     6541 ataacacaat tcgcttcact aatcaagttt aagacttctt ggatagatgg ctcattgagt
     6601 aaagaattgt aagttgccag acttaattgt tccggaacat aaagtgcgcg gtggttaccg
     6661 tttgctttag cagccattct tgcactgaca gaatttgcct gtacagttat cgcttcacca
     6721 atcccgcctc ttgcagggac aaacaggtta tgccgtttct tattttccag attagacaat
     6781 tgttcggcta ccacagccat agtagttcct cccatcacag cgatgatatt ctcgccttct
     6841 ggaagtagtc ggtctaaggc ttcgctcagc gcttcgccga atgcatcagc cactttggtc
     6901 tgctcttcgc tgtttcctga aacaacgata cagcgttggt taccaaaata ttctgccaac
     6961 tgttgttcgg tttgatgcgt acctaaaagc tgatccatga agttttcaag gctttgataa
     7021 acttcttcgc ccttagttgt cagcgtcata ccgctttttg aggtgtcgat caaattcaga
     7081 tttttcaaaa ggtctgtttc tgttcgtaag actcgttctg tgatccccat actttccgat
     7141 aaacttcgac gcccaatcgg ctgcatccaa tagatatttc gtaaaatttt atagcgttct
     7201 tgcaacactt cgataatatc tggtgcaaca gattcaatca tttttatttc atccagcata
     7261 gcgatctcct tagttggaca ctttacgtcc aatgtagact taaaacgacc catatacgat
     7321 aaaaaaagaa attaactatt cgaatttcac atcaataaag ggaaatctca acctcacgac
     7381 tgtaagtcta acagttttgc tctcgttttt caactaaaaa gactagaaaa agtgaaatac
     7441 tttttctagt cttttgactg attagttatc ataacgtttt ctttttgatg aactcgggat
     7501 tcctaataaa tcacgatatt ttgctactgt tcgtcttgag atcgctatct catctttttt
     7561 cagcaattca accaatttct ggtcagacaa cggcttgttt ttgtcttctt gatcgactaa
     7621 ttcctgcagc tttttcttcg ctgtatctgc tgaaaggtct tctgtctgtt ctgtactatt
     7681 cgtaggaatt cgtgtagtga aaaatttttt taattcaaat acaccgaagt ctgtttccaa
     7741 atatttcccg ttgactgcac gactgaccgt ggattcatgc acatctatct cagttgcaac
     7801 ttcctttaac gtaagaggct tgattggacg ttccttgtcg ataaagaaag ctttttgccg
     7861 ggaaacaatg acttgagcaa cccgcaaaat tgtatcccca cgttgtagga tcgttttctt
     7921 caaccattcg aattcctgtt tccgttcttt taaataattt tcagtttctt tatctgcttg
     7981 ttgtgacatt tgtttgaagt aactctcttg aaagcgtata tttggctgat tttttcggtt
     8041 tgagataact tgtacatttt tatcgtcgtc cactaatacc cggacatcag gaataatgta
     8101 caattcgctg ctacggtcga aaatccggcc agggcttgga ctcaacgttt gtaaatagtc
     8161 gaatatctgc tgaacagcat gtagatcaac agaaaaacgt tgtgcgattt ctttccattt
     8221 tcgctccacc agttcctcaa aacattcttc caacacgata taagctaatt ccggcgcata
     8281 atcatctcgt tctgtttgaa gcatgagaca ttcttgcaaa ctacgtgcac ctactccagc
     8341 tggttctaat tgctgaatca gagtcaacgc atcgagcatt tggataggcg tagctccaat
     8401 ctcttttccg gcttcttcta aactaatcgt caaaaatccg ttcaaatcga tgtattctac
     8461 aagataaaga acgattttcc tcaaaaaagt atctcggtaa ttcaaatgga cttgttcgat
     8521 caatgaatca aataaagacc cttttgtatc aggaatctga tttaggtaat ttgtctcttc
     8581 tccttcataa gaagatgaag aagcttttat gtaatctggc tgccattccg gctcaacaac
     8641 ttccaccaat ggattttcca ttgcttgatt ctcaacgaat gccaacagct cctcactgtt
     8701 atattgtaaa acttggatgg actgttgcaa ctgttgcgtc atggctagtt tctgtgtctg
     8761 ctgctgtttt tgtgaaaaat tttgctcaaa tttcattttt ttgtccaatc cccttgtaat
     8821 tttttatctg tttagttaca attatcccag tgcttgcgtc cttagtgtag tggatatcac
     8881 gtaagattcc ggttcttgag acgggggttc gattccctca ggacgcgttc tactgctatt
     8941 ttagcataat ttacgaatag ttgcttcttt atttttaagt gaaaactgtc cgccaaagaa
     9001 a
//
LOCUS       DAXWLF010000109         8944 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXWLF010000109 DAXWLF010000000
VERSION     DAXWLF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.109"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACGE4U_001938"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACGE4U_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFF8394067.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACGE4U_001939"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACGE4U_001939"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFF8394068.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACGE4U_001940"
     CDS             complement(2169..2906)
                     /locus_tag="ACGE4U_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFF8394069.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACGE4U_001941"
     CDS             complement(2997..3524)
                     /locus_tag="ACGE4U_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394070.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACGE4U_001942"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACGE4U_001942"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFF8394071.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACGE4U_001943"
     CDS             complement(4401..4886)
                     /locus_tag="ACGE4U_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFF8394072.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ACGE4U_001944"
     CDS             complement(5039..5236)
                     /locus_tag="ACGE4U_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394073.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACGE4U_001945"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACGE4U_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFF8394074.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACGE4U_001946"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACGE4U_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFF8394075.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
BASE COUNT         2530 a         1984 c         1498 g         2932 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttga
//
LOCUS       DAXWLF010000110         8921 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXWLF010000110 DAXWLF010000000
VERSION     DAXWLF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.110"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(632..1144)
                     /locus_tag="ACGE4U_001947"
     CDS             complement(632..1144)
                     /locus_tag="ACGE4U_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFF8394076.1"
                     /translation="MKNYYFFFLSCFCLFGLTSCGWIFSEEKTEVSLISKEKMIQAES
                     SSKESKRNQEDKTECNISEEIQYLLKNFGRKWVNYTDIYERNQSVKNYLTDRCIKENG
                     IDNDPHVQYKTIGRIHTITQDVIQSDQYLLFGEETTNEMTRFVLLQIKVDQEVQKIDK
                     IKIYYVRSAY"
     gene            complement(1195..2250)
                     /locus_tag="ACGE4U_001948"
     CDS             complement(1195..2250)
                     /locus_tag="ACGE4U_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8394077.1"
                     /translation="MSCLFLLFAIISMSSGESKHMDSAVGVTHTGEYIETLPIFSEIK
                     GRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRNDTNFFGITWFPGC
                     PYPQGSARGIGGSEGGFYMKFPNLNACYDYYGFMVASQSNFNQCVGNKDPGSCLLILG
                     RGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDAFAIKHWNSLPTKKTSLIAGKSKGNA
                     KVLDRIIGRPVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGEDYNWATYGWQVIM
                     NPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSNSGQSFSTVEQNAEQGRIMARY
                     QRSYNQAKIRSLVRKVK"
     gene            complement(2286..3074)
                     /locus_tag="ACGE4U_001949"
     CDS             complement(2286..3074)
                     /locus_tag="ACGE4U_001949"
                     /inference="COORDINATES: protein motif:HMM:NF024056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8394078.1"
                     /translation="MVFSVCFGVYNNLTSQDTHTIHETKVIDKQLKDMTGIESYVKNF
                     AFLYFTVSLESDQQINRSKAVESYLADGLQMDNEIDKDIETNVKVKNVSIWAIEEHES
                     KKHTFVVIFHVLLEIEKEFKERVYKVEVYRNELSYAIVKLPIVASNVKKATIKVDTRF
                     ETTSISRKEQEELEKFLYTFFRIYPKANAEELKYYGTTLLPIQQPLAFEELSNLQVAK
                     KEGRYEIHCNILYQDQETHLWFTMYYEMSMKQLENGNFRIEAIH"
     gene            complement(3221..5242)
                     /locus_tag="ACGE4U_001950"
     CDS             complement(3221..5242)
                     /locus_tag="ACGE4U_001950"
                     /inference="COORDINATES: protein motif:HMM:NF046089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFF8394079.1"
                     /translation="MKQWYSKVSFSITRRKLVFISLVIMMGVFFFHPIFVEAAGWIKE
                     TLGGKNAVYDFETYPLDNYNLDFFVDTSWNWLPWKWGEGISNTVIYGIFAVTNILWML
                     NVYLCYFLGFLVQEAFELDFLSELIGSLSHSIQNIAGMNRAGTLSHGLVPFFGGLLIA
                     LAGCSVVYQGIVKQHPTQAIQQAVTFLFTFICCGIFFMNTHTYLEKANEVQKEINSEM
                     LTIAKDLLPEQKGKPDAIKAIRENLFAIQIKQPWLVLQFGDSDIKTVGEERVINLLKE
                     SPYDEGEQRNKLVEEEVTKQKNGNLSVQKVYLRFGMVFLTLIVNIVISMGVLALVVIL
                     IASQVLFLLFIGFLPVAMIFSLFPNSGSLLGNALQKVFQLLFTKMGILLILTITFSIS
                     HELNHLSKNKGMIWTSFLQVTLWFSVSTRVNELLGWMKIGGAQTRAGDRTGRFLRGMM
                     LGTMGQKMIRGIGENFVGGAAGGVADQMISKSMSGQRSITTTPLIERVGKKVSAMTEL
                     PATIESRAEQWKRTIQYVPTNAQYKAQKMKYDYFSGRMNESERQTRARNLANERQKNF
                     YEARKDALQLEQQERKQQAMRSMAHQTGKTQVTSGKLQTRKSAMIGRTEQFPTIEKRS
                     NISNVAKNKKLSKQEHPIHERSTNRKIETPVRWTSTSCQNARKGRTKKE"
     gene            complement(5243..7780)
                     /locus_tag="ACGE4U_001951"
     CDS             complement(5243..7780)
                     /locus_tag="ACGE4U_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8394080.1"
                     /translation="MRLPKLVPKIPFYYLEDNLVFGEEDRVYAYYEFPMYSYSFVGED
                     QAYQLQQAIMRLIRQSYSSHVRFFFCTNEEKIEKTIKASKELVRSEGELKTIAYQHLE
                     GVQHILEELHGKYIQNVCWYVGIELVLEESAPKEAHWMKEFRIATKDFFHRSNARLFG
                     DFETIPNEKIQRYKRLENLLYNRISKYFRLRKTTPEDMVFLVEHLEGKKEQSLDEVMY
                     RPNSFKEETNTYTYHYDVLNLASAGITEKKNYLILEQGTEKEYITYLSLSRMTRDLVF
                     PFQSEIYYFLQSELDFPIDSWLDVNLLDNRLALAKVRGKKSDLSDIYESGMSVGKDVG
                     NNVREAYQSANELEQKLEDTREDLYVLSLNVRVSGKTYDEMLKRVSDVRNFFDVYSMY
                     LEVPCNVQLFLHFGSYPAGRRVIEDYRQYVGAEFIASLGIGATQQLGDGCGIPIGFNA
                     ETGIPVFIQPWLAAQGIKGTVTNTLAKALIGSLGGGKSLTENLLLFWSVLFGALAFCI
                     DPKSERSNWSEDLPYFAPYLKTVNITSEKENIGLLDPFQLMRRKKDQESLALDVLTYI
                     TGVSIRDEDRFPLLQRAVKTVSDFEGTKGLLLVIQELYAQEAAIAQRLAKHIESFKEL
                     SIAGLLFGDGTNQQGLNLDAKCNLALVQDLTLPDSETAPEDYNSSEILSIAIMMSLAT
                     YALDFIKQNRDIYKAVGLDESWAWLNVAQGKILGNKLVREGRSRNAGIDFSTQNTDDL
                     GDEKMRSKIGLKFIFRSNDREEITKALNFCGISVTEENIQRVKNLENGECLFCDIHGN
                     IGILYVYYWFEDLFHAFDTRPPMEKNYEEEEYKDEILEKLVEEIEVN"
     gene            complement(7802..8161)
                     /locus_tag="ACGE4U_001952"
     CDS             complement(7802..8161)
                     /locus_tag="ACGE4U_001952"
                     /inference="COORDINATES: protein motif:HMM:NF024062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFF8394081.1"
                     /translation="MNKIRNWTKIWYIERVFYKLWDGVALPRPVTQTFAIWFGIGFFG
                     GIFRIFPFVMEHTITRQFFCPWLFAFLMTRKQFQGRKPYNYLASFLLFQCRSKESFHG
                     QWVKPIEKKKSNEEFVV"
     gene            complement(8163..8381)
                     /locus_tag="ACGE4U_001953"
     CDS             complement(8163..8381)
                     /locus_tag="ACGE4U_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394082.1"
                     /translation="MNFFQNVFTDFVGDFKYLIMLAMLGGAAYIAFERKVSKAIPLIL
                     VLAVAVWLVGDTSGVFNWILDRMRSWGR"
BASE COUNT         2684 a         1915 c         1285 g         3037 t
ORIGIN      
        1 ggtaatgaac gttaatttta taaggtaaaa gctatgagtg taagagcaaa tttgttgaga
       61 taacgtgtag gtattcttat aatagacttg ggaatattcc tatatcactc tctaggggta
      121 gctcagggga aggagttgcc cctttaaatg tatattagaa taatcaagat aaccaggtgc
      181 tttgaaattc tgaatctatt tatccaattc aactgtgttt cctatagagt ttatacagga
      241 acccatcgat cataatcaat tgttctgaaa ttacatctaa ctgattataa atttgctcta
      301 caagctcatc cgcttttgta tgtagcgata gaaatttgcg tccgtgtatc tacttaggat
      361 cattttttca caaattcatc ccatgattca ctaaatcatg gttcaaaaag acatttttca
      421 actctagtac ttgttttaac caaagtactt ctactttttc cgtttctatc tcattcctcc
      481 tcctattttt tgtacattat tttcttatct ctttcctttt aattgattta ttcccaaaat
      541 tcttcatatt ctttaaacat cttaaatagt tcagcttctt ccttttcgtt aaactataat
      601 ttctagtaaa taaaacaacg tcatttttct tttaatatgc acttcttaca taataaatct
      661 tgattttatc aatcttctgc acttcttgat ccaccttgat ttgaagtaac acaaaccgag
      721 tcatctcatt tgttgtttct tctccaaata ataaatattg atccgattgg attacatctt
      781 gtgtgatagt atgaatacgg ccaattgttt tatattgtac atgtggatcg ttgtcaatcc
      841 cattttcttt tatacaacga tcggttaaat agttttttac agattgattt cgttcgtata
      901 tatccgtgta attcacccat tttcttccaa aattctttaa caaatattgg atttcttcag
      961 aaatattgca ttctgttttg tcttcctgat ttcttttgct ttctttactt gaactttctg
     1021 cttgtatcat cttttctttt gatattaagg aaacttctgt tttttcttca gaaaaaatcc
     1081 acccacaact agtaagtcca aacaaacaaa aacaacttag aaagaaaaaa taataatttt
     1141 tcacaatcga tccttctccc ttttatacta aaaattttca tcgaaagaat tcttttattt
     1201 cactttccga accagacttc gaattttcgc ttggttatag ctgcgttgat accgtgccat
     1261 aattcgcccc tgctcggcgt tctgttctac ggtggagaaa ctttgtccac tatttgagac
     1321 atctgttatg atacccgtgt ggccatagat tccaggagac aatgcaccac ctgcttgcca
     1381 attgaccaca tctcctgctt gtaagtcact gggctttgga ttcatgatca cttgccaacc
     1441 ataggttgcc cagttataat cttcccctat taacatcgca aacatgtgtc cagaacccat
     1501 taactgtggg ccacctagtt gagatacata ccatgccgta ccaccataac attccccatt
     1561 atttacgggt cgtccaatga tccgatccaa aaccttcgca ttgcctttac tttttcctgc
     1621 aataagcgaa gtttttttgg ttggtaggct attccaatgc ttgatcgcaa aggcatcata
     1681 ttccgttaag ttataggact ttatgatact cattgcgcct tgataatacg gactgccttc
     1741 ggtgatacct gccgccgcat aacctccacg acctagaatc aataaacaac ttccaggatc
     1801 tttattgccc acacattgat taaagttgct ttgactcgcc accataaatc cataatagtc
     1861 ataacaagca ttcaggttcg gaaacttcat atagaaacca ccttcagagc caccaatccc
     1921 acgtgcagaa ccttgtggat agggacaacc aggaaaccac gtaatcccaa aaaagttcgt
     1981 atcgtttctt ccagattgtg actgcccata agctgattca tatcctaatt gagaaagaat
     2041 cacactcggt aataactgat actttacagc tgcaccaact gctgcttggg catgttcatc
     2101 tgttatcgga ccacgccctt tgatctctga aaaaataggt aaggtttcga tatattctcc
     2161 cgtatgagta acaccaactg ctgaatccat gtgcttcgat tcaccactag acatgctaat
     2221 tatcgcaaat aataaaaaca ggcaactcat catacagatg ccactaaata aaaaaatctt
     2281 tttcactagt gaatcgcctc aattcgaaag ttcccattct ctaattgttt catagacatt
     2341 tcatagtaca tggtaaacca aaggtgcgtt tcttgatctt gatacaaaat attacaatga
     2401 atctcatatc taccctcttt ttttgcgact tgcaaattcg atagttcttc aaaggctaaa
     2461 ggctgttgaa ttggtaaaag agttgtccca taatatttta attcttctgc gtttgcttta
     2521 ggataaatac gaaagaaggt atataagaat ttctctaatt cttcctgttc ttttcgagaa
     2581 atgctcgttg tttcgaaccg agtgtctact ttaatcgttg cttttttaac attactcgct
     2641 acaataggca atttcactat ggcatatgat aattcgtttc gatatacctc caccttatac
     2701 acacgctctt tgaattcttt ttctatctct aataagacat gaaatataac cacaaatgta
     2761 tgtttttttg attcatgttc ctctattgcc caaatcgaga catttttaac ttttacattc
     2821 gtttctatgt ctttatctat ttcattatcc atttgcagcc catcagctaa ataactttct
     2881 actgcttttg atcgatttat ttgttggtcg ctttcaagac tcactgtaaa atacaaaaat
     2941 gcaaaatttt tcacataact ttctattcca gtcatatctt ttaattgttt atcaatgact
     3001 tttgtttcat ggattgtatg cgtatcttga gaagttaaat tattataaac tccaaaacaa
     3061 acactaaaaa ccaaaaagct ccagcctaca aaaaaaagga agcgcctttt tcgcgctccc
     3121 ttatagcttt cttttttctt gtctttcttt ccttttagtc ttgtttcttg ttcagagaga
     3181 atagacagtt ttttcttccg aggaaacata gccctcctcc ttattctttt ttagttctac
     3241 cttttctcgc gttttgacaa gaagtcgaag tccaacgaac cggagtttca atctttcgat
     3301 tcgtacttcg ttcatgaatc ggatgttcct gcttcgacaa tttcttattt ttagctacat
     3361 ttgaaatgtt acttcttttt tctatcgtag gaaattgttc tgttcgacca atcattgcac
     3421 ttttccgtgt ttgtaattta ccagaagtca cttgtgtttt tcctgtttga tgcgccattg
     3481 aacgcatcgc ttgttgtttt ctctcttgtt gctctaattg caatgcatct tttcgtgctt
     3541 cataaaaatt cttctgtcgc tcattcgcaa ggttacgcgc tcgtgtttgt cgttccgact
     3601 cattcattcg cccagagaaa taatcgtatt tcattttttg tgctttgtat tgggcattgg
     3661 ttgggacata ctgaattgtc cgtttccatt gttctgccct actttcaatc gttgccggta
     3721 attctgtcat agctgaaact ttttttccta ctcgctctat aagaggtgta gtagttatac
     3781 ttcgttgacc actcatagat ttggaaatca tttggtcagc tacacctccg gcagcaccac
     3841 caacgaaatt ttcaccaatt cctcgtatca ttttttgacc cattgtacct aacatcatgc
     3901 cacgtaaaaa acgtcccgtt cgatccccag ctcgtgtttg tgcgccacca attttcatcc
     3961 aacctaataa ttcattgaca cgtgtcgaga ctgaaaacca taacgtcacc tgtaagaaac
     4021 tggtccaaat cattcctttg ttcttggata agtggtttaa ctcgtgacta atagaaaaag
     4081 taatggttaa aatgagtaaa atgcccattt ttgtaaacaa taattgaaag actttctgta
     4141 aggcattacc taataacgat cctgaatttg gaaacaacga gaaaatcatc gctacaggta
     4201 aaaatcctat aaataacaag aacaatactt gcgacgcaat taagatgacg actaatgcca
     4261 atacacccat actaatgaca atgttgacaa tgagcgttaa aaacaccata ccaaaacgca
     4321 aatacacttt ttgtaccgaa agattgccat ttttttgttt ggttacttct tcttcaacaa
     4381 gcttatttcg ctgctctcct tcatcataag gagattcttt taataaattg attacacgtt
     4441 cttcaccaac tgtttttata tcgctatccc caaattgcaa tactaaccag ggttgcttga
     4501 tttgaatggc aaatagattt tcacgaatgg ctttaattgc atctggtttt cctttttgtt
     4561 caggtaacag atctttggca atggttaaca tttcactatt gatttctttt tgtacctcat
     4621 tggctttctc taaatatgtg tgcgtattca taaagaaaat gccgcaacag ataaacgtaa
     4681 ataaaaatgt cactgcttgt tgaatcgctt gcgtagggtg ttgtttcact atcccttgat
     4741 aaactactga acaacctgct aaagcaatta acaatccacc aaagaaaggg actaatccat
     4801 gactcaatgt ccctgcacgg ttcattccag caatattctg aattgaatga cttaacgaac
     4861 caatcaactc tgaaagaaaa tctaattcaa aggcttcttg gacaagaaac cctaaaaagt
     4921 aacacagata gacatttaac atccataaaa tattggtaac ggcaaatatc ccataaataa
     4981 ctgtattaga aattccttca ccccatttcc acggcaacca attccacgac gtatccacaa
     5041 aaaaatctaa attataatta tctaaaggat atgtttcaaa atcatatacg gcatttttgc
     5101 cgcctagggt ttcttttatc caaccagctg cttctacgaa tattgggtga aagaaaaaaa
     5161 cacccatcat tatgactaga cttatgaaaa ccagttttcg gcgagtaatt gaaaatgaaa
     5221 cctttgaata ccactgtttc atctaattca cctcaatttc ttcaaccagt ttttctaata
     5281 tctcgtcttt atattcttcc tcttcgtagt ttttttccat tggcggtcgt gtatcaaatg
     5341 catggaataa atcttcaaac caataataca catacaaaat gccaatatta ccatggatgt
     5401 cacaaaacag acattctcca ttttctagat tttttacacg ctggatattt tcttctgtga
     5461 cagagattcc acaaaagttt aatgctttgg taatttcttc gcgatcattc gaacgaaaaa
     5521 tgaatttcaa cccaatttta cttctcatct tttcatcgcc taaatcatct gtgttttgtg
     5581 ttgaaaaatc aatccctgca ttgcgagatc ttccttcacg aaccaattta tttcctaaaa
     5641 tttttccttg cgcaacattc aaccacgccc aactttcatc taagccaacc gctttataaa
     5701 tatctcgatt ttgtttaata aaatctagcg catatgtagc taaactcatc ataattgcta
     5761 tacttaaaat ttcagaagag ttatagtcct ctggtgcagt ttcactatct ggtaacgtta
     5821 aatcctgaac cagtgcgaga ttacatttgg catccaaatt taacccttgt tgattcgtgc
     5881 catctccaaa taataggcct gcaatagata gttctttgaa gctttcaata tgctttgcca
     5941 ggcgttgtgc gatcgctgct tcttgtgcat ataattcctg aatcactaga agtaatcctt
     6001 ttgttccctc aaaatcagaa acagttttta ctgcccgttg taacaaagga aaccggtctt
     6061 catcccgaat acttacaccc gtgatatagg tgagcacatc caaggctaaa ctttcttgat
     6121 ctttttttct tctcatcaat tgaaatggat caagtaaacc aatattttct ttctcactgg
     6181 taatattaac tgtttttaag tagggggcaa aatatggcaa atcttcggac caatttgaac
     6241 gttccgattt tggatcgata caaaaagcca aggcgccaaa caacaccgac caaaataaca
     6301 agagattttc agttaaggat ttcccaccac ctagcgaacc aattaatgct ttggcaagag
     6361 tattggtaac cgttcctttt attccttgtg ccgctaacca tggttgaata aacactggga
     6421 ttcctgtttc tgcattaaat ccaatcggaa taccacagcc atctcctagt tgttgtgtcg
     6481 caccaatgcc aagactagca ataaattctg cgcctacata ttgacgataa tcctcaatca
     6541 ctcgccttcc tgctggataa cttccaaaat gtaaaaacag ctgtacattg cacggtactt
     6601 ctaaatacat agaatagaca tcaaaaaaat tgcgaacatc actcacacgt tttaacattt
     6661 catcataggt cttccctgac acgcgaacgt ttagtgataa tacatacaaa tcttctcgcg
     6721 tatcttctaa tttttgttcc aactcattgg ctgattggta cgcttcgcgt acattgtttc
     6781 ctacatcttt tccaaccgac ataccacttt cataaatatc agataaatca ctctttttgc
     6841 cacgtacttt cgccaaggcc aaacgattgt ctaaaagatt cacatctaac caagaatcga
     6901 ttggaaaatc caattccgat tgtaaaaaat aataaatctc tgattgaaat ggaaatacca
     6961 aatcacgggt catacgtgaa agagataaat acgtgatata ttctttttct gtcccttgtt
     7021 ctaaaattag ataatttttc ttttctgtga ttcctgcact ggccaaattc aatacatcat
     7081 agtgatacgt atacgtattc gtttcttctt taaagctatt gggtcgatac atcacttcat
     7141 ctaaggattg ttcttttttt ccttctaaat gttccactaa aaacaccatg tcttctggtg
     7201 ttgtttttcg taaccgaaag tatttactaa tccggttata caataaattc tccaatcgct
     7261 tatatcgttg aattttttca tttgggatgg tttcaaagtc tccaaacaaa cgggcattgg
     7321 atcggtgaaa aaaatctttt gtcgcaatac gaaattcttt catccaatga gcttctttgg
     7381 gagcactttc ttctaatacc agctcaatcc caacatacca acaaacattt tgaatatatt
     7441 ttccatgtag ctcttctaaa atgtgctgaa caccttctaa atgttggtag gcaatcgttt
     7501 ttaattcccc ttcagaacgc accaattctt ttgaggcttt aatcgttttt tcaatttttt
     7561 cttcgtttgt acaaaaaaag aaccgaacat gcgaagaata actttgacga atcagacgca
     7621 tgattgcttg ttggagttga tacgcctgat cttctcccac gaagctatat gaatacattg
     7681 ggaattcata ataagcgtac acacgatctt cttctccaaa aactaaattg tcttctaaat
     7741 aataaaaagg aatcttaggc actagttttg gtagtcgcat ccttttcacc tccataacgt
     7801 actacacaac gaattcctcg tttgatttct ttttttctat tggctttacc cattggccat
     7861 gaaaggattc ttttgaacga cactgaaata aaagaaaaga agccaaataa ttataaggct
     7921 tccttccttg aaattgtttt cgtgtcatta aaaaagcaaa taaccacgga caaaaaaatt
     7981 gtcgagtaat cgtatgttcc ataacaaatg gaaaaattcg aaaaatcccc ccaaaaaagc
     8041 caatcccaaa ccatatcgca aatgtttgtg ttacgggacg aggtaacgcg accccatccc
     8101 acaatttata aaacactcgc tctatatacc aaatctttgt ccaattgcgt attttgttca
     8161 ttttatcgcc cccatgaccg catacgatcc aaaatccaat tgaatacgcc agacgtatct
     8221 ccaactaacc aaacagctac tgctagcaca agaatcaatg gaatggcttt gcttactttc
     8281 cgttcaaagg caatatatgc cgcaccacca agcatcgcca acataatcaa atacttaaaa
     8341 tccccaacaa aatcagtaaa cacattttga aaaaaattca tttgcttttt cctccctgtt
     8401 aatactcatt gaactaatga cgatacacaa taccacagag caaaactgat aaaaatttca
     8461 cttgatattc ctcattatgc attttttccg ttccttgatt gctgtttttt acgtaagaag
     8521 tataaataat ttctcacacg acgatacatt acttttacag gaacatgcgt acaataaatc
     8581 cggtgctgcg tacttttctt tgttttagga tcaagtttca tagaatagaa atcataaaaa
     8641 tacttaccac tttttttatc gtatgttcct tcaatcacca gtgaaaagct agggtggtaa
     8701 atctaataaa tacaattgat acttccctct actagataaa gggataaaac caagtacctt
     8761 taaatgatct gatacagtca taaacacgat ctatctcctc ccaaataaaa caaaaaaagg
     8821 ctgtaatcgc attttgatta cagtcttttt tgttctcttt ttataaatgt actaattaaa
     8881 ggtttacttc cggtcttttt atttaataaa tcaattttcc c
//
LOCUS       DAXWLF010000111         8882 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXWLF010000111 DAXWLF010000000
VERSION     DAXWLF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.111"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..1101
                     /locus_tag="ACGE4U_001954"
     CDS             <1..1101
                     /locus_tag="ACGE4U_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFF8394083.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1609)
                     /locus_tag="ACGE4U_001955"
     CDS             complement(1280..1609)
                     /locus_tag="ACGE4U_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8394084.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACGE4U_001956"
     CDS             complement(1599..1862)
                     /locus_tag="ACGE4U_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFF8394085.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACGE4U_001957"
     CDS             complement(2322..3125)
                     /locus_tag="ACGE4U_001957"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8394086.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACGE4U_001958"
     CDS             complement(3103..4227)
                     /locus_tag="ACGE4U_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8394087.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACGE4U_001959"
     CDS             complement(4243..5058)
                     /locus_tag="ACGE4U_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8394088.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACGE4U_001960"
     CDS             complement(5048..5920)
                     /locus_tag="ACGE4U_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8394089.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACGE4U_001961"
     CDS             complement(6005..7294)
                     /locus_tag="ACGE4U_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8394090.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7971)
                     /locus_tag="ACGE4U_001962"
     CDS             complement(7438..7971)
                     /locus_tag="ACGE4U_001962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394091.1"
                     /translation="MANEKATMTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQL
                     SWAAIWGKHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAR
                     EVGQQLGLTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGV
                     KRFGDGIHDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACGE4U_001963"
     CDS             complement(8017..8160)
                     /locus_tag="ACGE4U_001963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFF8394092.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(8643..>8882)
                     /locus_tag="ACGE4U_001964"
     CDS             complement(8643..>8882)
                     /locus_tag="ACGE4U_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8394093.1"
                     /translation="MRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSK
                     IRNEVDKKLFQARVTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2749 a         1901 c         1406 g         2826 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacagccaga ctgtagacaa aagctgttcc
     8701 aaactcgtaa ctcgagcttg gaacagcttt ttatctactt cgtttcgaat ttttgaacaa
     8761 aaaaataaag gctccttgcc tttccatagc caggtggcta atttctttaa attcattgca
     8821 gcaaacacta gcatcgtatg cgtcgcgact ttttccaagc ctcgatactt ggtccaacgc
     8881 at
//
LOCUS       DAXWLF010000112         8848 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXWLF010000112 DAXWLF010000000
VERSION     DAXWLF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.112"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            27..100
                     /locus_tag="ACGE4U_001965"
     tRNA            27..100
                     /locus_tag="ACGE4U_001965"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:61..63,aa:Ile,seq:cat)
     gene            complement(485..982)
                     /locus_tag="ACGE4U_001966"
     CDS             complement(485..982)
                     /locus_tag="ACGE4U_001966"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFF8394094.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1022..1831)
                     /locus_tag="ACGE4U_001967"
     CDS             complement(1022..1831)
                     /locus_tag="ACGE4U_001967"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8394095.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2165..3022
                     /locus_tag="ACGE4U_001968"
     CDS             2165..3022
                     /locus_tag="ACGE4U_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFF8394096.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            3015..3917
                     /locus_tag="ACGE4U_001969"
     CDS             3015..3917
                     /locus_tag="ACGE4U_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFF8394097.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3950..4300
                     /locus_tag="ACGE4U_001970"
     CDS             3950..4300
                     /locus_tag="ACGE4U_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8394098.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4349..5272
                     /locus_tag="ACGE4U_001971"
     CDS             4349..5272
                     /locus_tag="ACGE4U_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8394099.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5350..6243
                     /locus_tag="ACGE4U_001972"
     CDS             5350..6243
                     /locus_tag="ACGE4U_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8394100.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6281..6844
                     /gene="amaP"
                     /locus_tag="ACGE4U_001973"
     CDS             6281..6844
                     /gene="amaP"
                     /locus_tag="ACGE4U_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8394101.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6858..7046
                     /locus_tag="ACGE4U_001974"
     CDS             6858..7046
                     /locus_tag="ACGE4U_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8394102.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7059..7961
                     /locus_tag="ACGE4U_001975"
     CDS             7059..7961
                     /locus_tag="ACGE4U_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8394103.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7993..8235
                     /locus_tag="ACGE4U_001976"
     CDS             7993..8235
                     /locus_tag="ACGE4U_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8394104.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8491..>8848)
                     /locus_tag="ACGE4U_001977"
     CDS             complement(8491..>8848)
                     /locus_tag="ACGE4U_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394105.1"
                     /translation="YFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLLLFVCL
                     DYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKVVVLFC
                     MFTFPISRKFREALFY"
BASE COUNT         3081 a         1535 c         1725 g         2507 t
ORIGIN      
        1 taacaaaaat aaattttatt tatgatgtac ctttagctca gttggttaga gcagacggct
       61 cataaccgtc cggtcgtagg ttcgagtcct acaaggtaca taagaggttg tgaaaaagct
      121 gccctgcatc aagtaataag aacagccaag taaaaacagc ttcttatgtt tttacttggc
      181 tgttcttatt actgcagatg caagcttttg aataccgttt aaggaagagg ttgtgaaatc
      241 agcgttttga cctgagtatt agcttaacaa acggaaaaat agttcctcat attttgccgt
      301 ttgttaagct aataccgagg gtcagctgat tgaacaccat ttaagaaaga ggtcgtgagc
      361 ctgccgtttc actctgtgtc ataaaaaagc caatctactt tgatagaaaa agcacgtagg
      421 actaaaacag ttctgcgtgc tttttttatt gtgtgttaaa tatcgtaaat gaagatagat
      481 atttttatga ttcgacgata gagacgttga aatctttcaa cagctctaaa gcggattctc
      541 cgtgttttct actggagact aacattggat caacaaaaaa gtctgcttca ggaaaagcac
      601 tctgcaggcc tatcagatta gagagtacac acaagtttgt ttcaatcccg actacttcga
      661 tctctttttg ttcttctttg ctttcaaata aagtttcctg gatttcagct aaagtttcag
      721 gtggaatcgt aaagtaatac tttttaatca ctcgttcatt ttcttgtgga gaaagaagag
      781 ggatcaattg caaatcttct cgttcttctt ctttgtcctt cagttcaact ggtatatccc
      841 gtgtgaagag aatatattca ttattttggc gtgctttagc taatctttga gcaatacgtt
      901 ctgccaattg atctgactct ggaatataaa attcgctgtt tggatcaaga atatgatttt
      961 gcatatcaat aataactaac attttctttc ctcctacatt catatcgtca ttttattctt
     1021 tttaatagaa gttttttaaa atataagcaa caccagcttc ttcattcgtc agtgttactt
     1081 ggtttgcaat ttcttttact tgtcgctgtg cattccccat cgcaatactc aacccgctgt
     1141 aattcaacat agcaatgtcg ttttcttgat cgccaaaagc catgatgtca ctcgattcga
     1201 ttccaaggta atccgccaga aaaacaagtg aatgagcttt agtgatacct gtaggcgtga
     1261 tttctaagaa atgcggatca gagaatactg attggatacc atctgcttgc agatctcttt
     1321 gtagcttctg tagtttttct tcactcttat ctgttaatgc taatttaaga tagctaagag
     1381 aaggatcttc caacatattt aaaatttgat caaaagaatg gttctgtaaa ttgaaatgtt
     1441 cataataagg gtcatctttg actagatttg attcatcgaa tgtcagctga tcgtcttggg
     1501 tttctcggct agcaatgatg gatattttcg cttcttgagc atagaagaag gcttttctca
     1561 gtgtatcgct atctattttt gaacgtctga gaactttcgc catttttcca tccttcattt
     1621 cagcaatcag tgaaccattg gaagaaatca aatagccacc gattcctaat tcggaaaaaa
     1681 tatctttagc agaaatgctg ttccttccgg tagcgacggt tacgattccg ccattttcat
     1741 caaatttccg aagtgcttgg atattttctt ctggtatctt tccatttcga tcaagcaagg
     1801 taccgtctaa atcaatcgct gctagtttca ttttatcttc ctactttctt tttacatatc
     1861 tattttcctg ttaccttaag attaaatgag aatttaaaaa aatgaaacta acttgctttg
     1921 tcattatgaa ttttttcaaa aaacattctt aagaaaaagg tggctatata tagtagtttt
     1981 tggtcaatac ctttgaataa aaagccatga aaaacgaata gttagcgctt accgatgcgt
     2041 tttgtgataa aaaaatttta aaatattcaa tgaaaacaaa gcatatattt tgctttaaaa
     2101 ccaaatgagg aatattctag atgtagaaca ggttagaaaa agattagaaa aaggagtgat
     2161 tttgatggag aagagatacg gcgtagttcc tgtggtaact gcttcagacg aaaattatgc
     2221 tccttacttg agcgtgatga tcgcaactgc attagaaaat tgcaataaaa caagacgcat
     2281 aaaattttat gtgattgacg atggtctatc agaatatagt aaagaaggat tggaagaaac
     2341 ggtaaataaa tattcaagta atgcaagtat ccaattcctg acagtggaaa aagatatcta
     2401 cgaagacttc ctagtcagtg accatatcac aactaccgct tacttaagaa tttctttacc
     2461 aaacttatta gctaaagagg attataagaa agtcctttac ttagattcag atgtgttagt
     2521 actagacgat atcgtaaaac tatatgatga gccgttaaat ggaaaaacaa tcggcgctat
     2581 tatcgatcca ggccaagtga aagctttgga aagattagga atcgattccg atgacttgta
     2641 cttcaattca ggagtgatgg tgattgacat tgatcaatgg aataaaaaag aaattacaga
     2701 aaaaactatc cattacctca gtgaaaatgg cgatcgaatc atctatcacg accaagatgc
     2761 tttgaatgct gtactttatg aagattggga acaacttcat ccaaaatgga acatgcagac
     2821 ttcgctgatt tttgaacgtc atccagcacc aaatgaaaaa tatgaacgtc agtataaaga
     2881 aggaaatgaa aaaccttcga tcgttcactt tacaggacat gataaacctt ggaatacgtt
     2941 gaaagatcat ccgtatacca atttatactt aaaaaaatta gcacacagta ctctaacgaa
     3001 agtaggcgag atcaatgaat aaaaaagaaa tagcagtagt cgcaagttgt aacacaaaat
     3061 tcgtaccaca tttagctgca ttatttgtct cagtattaga taattgcaac ccgtctaaat
     3121 ttgttcggtt ctatgtgatt gacgatgata tcgattttga aagtaaacag cttttacgct
     3181 tctcagtgaa aaacgcacga atgaactcag atgtggaatt cttgaaaatc aataaagaat
     3241 tctttacaaa tgtggtaatc agcgatcgta ttccagaaac agcttattat cgtattgcga
     3301 ttccggaatt gttccgcggc acagaagttg aacgtatctt atatatggat tgcgacatga
     3361 tcgctttaca agatatctct aaattatggc gtcttgactt cggtgattca atcgtagcag
     3421 ctgttgaaga cgctgggttc catcaacgac tggaaaaaat gaaaatttca gctaaatcaa
     3481 tgcgttattt caactcaggt ttgatgctga tcaatgtgaa aaaatggtta gatgaaaata
     3541 ttacgcaaaa agtattagat ttcatcgaac ataatccaga aaaattacga ttccatgacc
     3601 aagatgcatt aaatgctatc ttacatgacc gttggttacc acttcatccg cgttggaatg
     3661 cacaaggcta tatcatggca aaagcaaaaa aacatccaac agcagcaggt gaacgtgaat
     3721 acgaagaaac tcgcaacaat ccttacatta tccatttttc aggacatgtg aaaccgtgga
     3781 gcaaggattt tgaagggcca acgaagaaat attacgaaaa atatgcaggg atgacggctt
     3841 tccgttgtgt agctaaattc cctaaatatc caaaatatgc aaaagttcaa ccaaaaacag
     3901 atgtagttgt taaataatag aagaccacaa ctgaagaggg ggagtaggaa tggaaattac
     3961 aatgtctaaa aatgcagtag agacgttaat tgaaaaggtt ggggaaaata caaaaatcgc
     4021 actcgcgctt atcaatgatt ccgatccatt tctaagggac aaaggtgctt ttgcaaaagg
     4081 cagctttttt caaatcattc cttttgtttc ggaatttgga gaatatgcga ctaaaatcga
     4141 gcatccatta ttggacatct atacatctaa attagagcaa aattatttcg gtaaacgatt
     4201 gaacatggac ttcaacaaac agcttgacag cttttcatta gaaaatgaaa tcgcagtact
     4261 cgattataat atcaaattaa agaactgctt cttcagctaa tagtttcgag aaagtccaaa
     4321 aaggaggtat acattgagat actactttgt ggatgaagaa aagaatgtaa tagaaacaga
     4381 tgaaaaaaaa tataattggc tcgtacttga tagtgcaaag cccgaagaga ttgagcaagc
     4441 aatcgaagaa ttcgagcttc ctgatgatat tttcgtagga accgaatatc cggaagaagt
     4501 ttctcggatg gagcgccttt atgagacgaa actagagcat ccattttctt tggtagtgat
     4561 taatctagat cagtcagata atcgtatcga aaaaaaatta acgccaatat cttttgtact
     4621 ttccgataat ctcttgatta cttgtatcga tagaaaaacc gaatttgtgg atcggctgct
     4681 gacaaagcat ggaaatcatt taaactcgtt tgaaaagatc atcacgtata cattattaga
     4741 tatttacacc cattacgtga aagaactaag agagatgaaa aagcgaattg acgctttgga
     4801 taaagcagct cgtaaaacaa cggaaaatga agaactgtat aaacaggcag atcttgaaag
     4861 agacatcgta tatatcgatc ataccttaag agatcaacgg gaaacaatgg atcaattatg
     4921 gaattctgaa actttcatca aacgtttaga cgatgaacag ctactctatg atgtccattt
     4981 gagacagcga caaacagaaa aaatgatcga gatctatcga gatttattag agagtattgg
     5041 tgatctattc aacgggatga tggacaataa tctaaatcat atcatgaaat atctagattc
     5101 agctgcatta atcatttccg ttccggcgtt gttcgcaggg atttggggaa tgaatacagg
     5161 aggattaccg ggtaaaagtt ctgctctagg ctttatactc gtagtctttg gctctttgat
     5221 agcagcagta gttttaggtt accatttata ccggaaagat tatacaaatt aaagaatcta
     5281 tttaaaacag gtatcaaaca aagaaatttg ttttggcaat acaatttaaa aactaggagg
     5341 aatacagcaa tgacagaacc aaaattaatc gacccgcgaa acttgtacca tacggagaaa
     5401 tttcctaaac aagatcagga tacaccagct ttacagcaga atatgattcc tgttcctgat
     5461 tgtggagaag aatcctatga aggcaataat caactagaaa accgccgtgt actaatcact
     5521 ggtggagatt cagggatcgg tcgtgcagca gcgattgcat ttgctagaga aggggcagat
     5581 atcgctcttc aattcttccc aggagaagaa aaagatgcag aggaagtagc aaaatacgtg
     5641 aaagatgcag gacgtaagat tgtcttacta ccttttgatc ttcgtgacga acaagcacct
     5701 aaagaaatca tagataaagc cgtgagtgaa cttggcgggc tagatacgat ggtactaaac
     5761 gcaggacagc aaatttcttg tcagtctatt gctgagttac cacttgagca agtcaaagac
     5821 acatttatga taaacatcat cgctatgttt gctttagtga aagaagctgt tccgcatatc
     5881 ccagcaggag gagcaatcgt aacaacaact tctgttcaag cattcaatcc aagcgaacat
     5941 ttattagatt acgcagcaac aaaagcttca atcgctaact ttacgatcgg tttagcaaaa
     6001 cagcttgcac ctaaaggaat ccgtgtaaat ggtgtagcac ctggtccaat ctggacacca
     6061 ttacaactag acgaaggaca accttctgat tcgatccctg aatttggtca gcaaagttta
     6121 ctagaacgtg caggtcagcc aggagaatta gcaccagttt atgttttctt agcatcgaat
     6181 aaagcaagtt atgttacagc ccaaatttac ggagtaactg gtggagaagc gatcaactta
     6241 taagaagttg atcgtttcac ccctatgagg aggtgcgaaa atgaataaag gtgtaaaaac
     6301 gatcggagtg atcggaacat tacttttgct atctgtgctt ttgtttgcga tactattaag
     6361 tgcaacctat ccattgcctt ttcagctaga gaagtttcga ttcttttcca tcactaattc
     6421 atacgtgcaa caatatgtat tttggcttgc cgttgcattt gctattttga caattatcac
     6481 gttacttgtt ttcctgtttt atccaaaaac aatcggaaac tttctactga aacatggtga
     6541 tggggagttg acgttggata aaaaagcaat tgaaggaatg gttcgttctc acttgcatga
     6601 agatgaattc atccattcac caaaagtaaa tgtcaaagca acgaaaaaca agatcgccgt
     6661 caatgtaaaa ggggagttga aacgaacttc agcactcatt ggtaaaacag gaacattgat
     6721 gaaagaaatc gaaaatgaag tacaacacat attgggaaca aaagagccag tgaaagtatc
     6781 ggtgaaatac acaggctttg aagaaccagc taaacgaagc aatgaccgca aacgagtaga
     6841 ataggggggg accaaaaatg aaagaactat tttcgacata caagctccct atcatctttg
     6901 gggtacttgg attgatatta gccgttttat ggatcaccgt gggctttttc aagaccttat
     6961 tgatcttgat catggcagcg atcggcgtcg ctatcggttt ttatctgaaa caaacgaaaa
     7021 tcttagatca ctattttaaa tcttaggagg aaacatccat gaataacaat aacaaaaata
     7081 ataacaataa aatgaacaac caaaaaccag tgtctgcaca tccagaccca acgacaccaa
     7141 accaatcaaa tggtgtgaac gatgaaaaga acaaaacagc accagtatct gcacatccag
     7201 atccagtagt ccctaacgtt caacaaggag tagaagagaa gaaacatgat gcagaaaagc
     7261 atgcacctca tcatccagga actccagaat ctaaagttcc agaacaacat gatgcaatcg
     7321 atccttctaa acctgtggaa gagcaaaaac atccttctga ccatgcgaaa gcacaagaaa
     7381 aacttagttc tgaccacaca aacggacaag gaaaacttgg ttctgatcat acaaataacc
     7441 aaggaaagca tgattctgaa caggaagatt caatgcgcgg tgagctgaat tatgaagata
     7501 aagttgtgca aaaaatcatc ggaatcgcaa ttgaacaagt agacggttta ttatctgcaa
     7561 acggtggttt cttctctaat gtggcaggaa aacttgtgaa tacagataat gtaacggctg
     7621 ggatcgaaac agaagtcggt aaaaaacaag tagcagttga tatggatgtc attgtggaat
     7681 atggcaaaga tatcgaaaaa atctttgaag aaatgcaaga agtaatcggc aaagaagtaa
     7741 aaaatatgac acatttagaa gtgatcgaag tcaacgctaa cgtagtggac atcaaaacaa
     7801 aagaagaatt tgaaaaagat caagaaacgg tgcaagacaa agtaactgac gcagcgaaaa
     7861 aaaccggtga atttgcttcg aatcaaacag ataaagtaaa atcagcagct ggaagcggcg
     7921 cacaaaaagt aaaagaaaat acagaaccac gggttcagta ataaggaagt aaaacattag
     7981 gaggaacgaa acatgggatt tatttggtca cttatcgtcg gaggaattat cggagcaatt
     8041 gcaggagcaa tcacgaataa aggttcttcg atggggatta ttgcaaatgt cgtagcaggt
     8101 ttagttggat cagcacttgg tcaagcatta ttaggttctt ggggaccaag tattgcagga
     8161 atggcaatcg tgccatcatt gattggtgcc atcattttag tactaatcgt atcattcttt
     8221 gctcgaaaac tgtaaaaaga cttttaaaaa gaggtcgtga aaaagctgtt cttcatcaag
     8281 taataagaac agccaaacaa aaatagcttc tcatattttt catttggttg ggcatattat
     8341 cgaagatgca agcttttgaa cactgtttat acggaataag aggctgtgac aagacttttg
     8401 tcacagcctc ttatttatgc gggcaaattc ccaattagat ttttaaaagt ttttccttaa
     8461 atgtgtcttt attgatacag agaagcttct ttaataaaac aatgcctccc taaattttct
     8521 tgagatagga aaggtgaaca tacaaaataa tacgaccact ttgaaaaccc agatgatcca
     8581 caaatggatc gtatgatact gatgaagtaa aaagcaatgg atcagaaact caaagagatt
     8641 gaaaaagaca aggaagaaat aatatctctt ttttactctt gtctgtttta gccaataata
     8701 gctcgtaaag taatctagac aaacaaatag aagtaaacaa agtttcgtaa gagcagcaat
     8761 cgtatcatgt tgctctgcta gacgattgac gagcaggaaa aatgttggta atggaatata
     8821 gatcacgagt aatgaaagaa aaaagtaa
//
LOCUS       DAXWLF010000113         8837 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXWLF010000113 DAXWLF010000000
VERSION     DAXWLF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.113"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..665)
                     /locus_tag="ACGE4U_001978"
     CDS             complement(<1..665)
                     /locus_tag="ACGE4U_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8394106.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLE"
     gene            799..1086
                     /locus_tag="ACGE4U_001979"
     CDS             799..1086
                     /locus_tag="ACGE4U_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394107.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1502..3040
                     /locus_tag="ACGE4U_001980"
     CDS             1502..3040
                     /locus_tag="ACGE4U_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFF8394108.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3037..4002
                     /locus_tag="ACGE4U_001981"
     CDS             3037..4002
                     /locus_tag="ACGE4U_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8394109.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3989..5311
                     /locus_tag="ACGE4U_001982"
     CDS             3989..5311
                     /locus_tag="ACGE4U_001982"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8394110.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5374..5478
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5552..6295
                     /locus_tag="ACGE4U_001983"
     CDS             5552..6295
                     /locus_tag="ACGE4U_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8394111.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6310..7302
                     /locus_tag="ACGE4U_001984"
     CDS             6310..7302
                     /locus_tag="ACGE4U_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8394112.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7314..8051
                     /locus_tag="ACGE4U_001985"
     CDS             7314..8051
                     /locus_tag="ACGE4U_001985"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8394113.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8041..8763
                     /locus_tag="ACGE4U_001986"
     CDS             8041..8763
                     /locus_tag="ACGE4U_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFF8394114.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2939 a         1513 c         1856 g         2529 t
ORIGIN      
        1 tgctctaact gttcaagagg atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc
       61 aattggggca aaacagatcc aatttccaga tacttcactt tattcaatgt ataagataga
      121 atagaacagc taagtaatgt atacagaaaa tgtgtgagat ccaaataaag caaggagaca
      181 actaaaatac caaaagtaat caagaaaaga cttttgccaa tcttaatatg aaaatatttt
      241 tcaatggctt gataaatgat atctgttccg cctgtgcttc cgccgaatcg aaagacgaga
      301 ccgcaaccaa tgccagaaag aatacctgtc atcaaactat tgatcagtag actatctaca
      361 gcaaaatgta tatgaaggct ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg
      421 acgatcgtcc ggagaaaagc gtgttttcca aaaagaaaca atgagataag caataaaggt
      481 aaattaaaaa gcagtgaagt gacagaagga gcgaatccaa aaagattatg caaaagcaaa
      541 gcaataccag taaccccacc atctgccagc ttagatggaa tattgattcc tgtcacagct
      601 aatccgtaga ttcctgcgcc taaaatgatt ccgcacaatt ctgtaaataa atttaatttt
      661 ttcatttcta gcctcttttc ttttgaaaat tttcagcaac ttctttatta tactgactta
      721 tactcttact tcaagaggaa aatgaaaaaa aattattttt ggctgtttct ttttatttat
      781 caaataagga gtggaaaaat gcgagcagca gacttattga caattcttac aaaatcaccc
      841 aaaaaaacag agtatcaagg agtttttttg gctcaagaac aaaaactggt gcctgtgaca
      901 atggtccaga taaataaagc aaatcaattt atctttttat ttgaacacgg caaagcacca
      961 cttgctatga aagacatctt tctttttttg atgaagaacc gtgagaaagc tgtttattat
     1021 cagtcaaaag aagaactcat tcctctttat ggagtgaaag agatagaaaa taaagtggtg
     1081 atataagtaa aaataagaaa acaagacgtt tatgatgtca aaacaaccat tttttacatt
     1141 tttggcaaaa aagtgtcatg atacagtata atattcgttg aacaggccaa taaaggtgtt
     1201 ctcccccacg agatttctac tttattggct gtttcttttt attaaaataa aagttgtgtg
     1261 tgtaaaaaag atatagctga ttccaagtga caatttcgta tagattttta tggtattcgt
     1321 agtgaaaaaa cgagaaccaa aaagttgatc cgactttgtt gcttggattc tttttttgtc
     1381 agcatattta gaggtatttt tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca
     1441 agttagttgg gaaaaaggaa agaaatgttt atacttaaag aataacagaa aggaaggtcg
     1501 catgaataat tttatacaaa cattaagtga aagaaaaggc gagttgctgg aagctgcatt
     1561 tcaacatctg tccatttctc tcatttcttt gctgattgca gcactgattg ctattccttt
     1621 agcgatttgg gcagccaatc ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt
     1681 gctccaaacg attccttcac ttgccttatt aggtttactt atcccatttg tagggatcgg
     1741 aactgttcca gcattgattg cacttgttat ttacgctttg cttccgattt tccaaaacac
     1801 gtatattggt ctagctgaaa ttgacccgtc tattgaagaa gcagcggtag catttggtat
     1861 gtccagaatg agacgcttgc tgaaagtaga gcttcccatc gcattacctg tcattatctc
     1921 aggtatccga actgccttag ttttaatcat cggtacggct actttggcag cattgatcgg
     1981 agcaggtgga ttagggacat ttattttgtt aggtattgac cgaaatacac ctgttttgac
     2041 attgattggt gcgatcagtt ctgccttgct tgcaattata ttcagcggac tgattcgctg
     2101 gttgcagcat aagaaacctc gatattccat cattacttta gcagcagtgg tgtgtttatt
     2161 tggcggcgtt tctttatatc agtctgcctt gtttaaaaat gaaaccatca cgattgctgg
     2221 aaaattagga gcggaacctg aaatcctgat ccaaatgtat aaagaattga ttgaagacaa
     2281 cacaaaaaca gaagttgaat taaaaccgaa ttttggcaag acgagttttt tgttcaatgc
     2341 actggatcaa ggtcaaatcg atatctatcc tgagttcaca ggtactgtcc tagaaagctt
     2401 ggtgaaggta cctgaagcaa tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga
     2461 agcagaagaa ttattgaata agcaattcga tatgacatta ttaaaaccaa tggcttacca
     2521 aaatacgtat gccttggcag tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat
     2581 ttccgatttg gcagctatct ctgatcagat caaagcggga ttcacactgg aatttattga
     2641 tcgttcagat ggatataaag gaatccaaga agcatatggc ttgaattttt caagtgtaca
     2701 aagcatggag ccaagtttgc gttaccaagc aatcaataat ggagatgtca atattgtgga
     2761 tgcctattcc acagatagcc agctcaagga gtacgatcta gtgactctag aagatgatcg
     2821 agggttattc ccagcttatc aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca
     2881 tcctgaaatc gttaaagcac tgaataaatt aagcggaaaa ataacagagg atcaaatgat
     2941 tgatatgaat taccaagtca atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt
     3001 cctgaaaaat gaaggattag taaaggagga agatcagtga ctaaattaat cgagttcaaa
     3061 catgtgcaaa aaaagtatga tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa
     3121 aaaggagaaa tctttgtttt ggttggtcct tccggtagtg gaaaaacaac tactttgaaa
     3181 atgatcaatg gattatctaa accatcagca ggagacatct acttcaaagg aaaatcgtta
     3241 aatgaatata atctgcagaa aatgcggtgg aatatgggat atgtgctgca gcaaatcgct
     3301 ttgttcccga ccatgacagt caagcaaaat attgaagtca tccctgaaat gttaggttgg
     3361 gaaaagcaga aaagagcaga ccgtgtagat gaacttcttc aaaaagttgg cttatctcct
     3421 gatatttatc gagatcgtat gcctagagag ttgtctggag gagaacagca acggatcggg
     3481 atcatccgag ctattgctgc aagcccagat gtgattttga tggacgagcc atttagcgca
     3541 ttagatccga tttcaagaaa ctcattacaa gaattggttt tgtccttaca cgaagaattg
     3601 ggcacgacca tcgttttcgt tacgcacaat atggaagaag caatcaagct aggtgatcgt
     3661 attgcattta tgaaggatgg agaaattatc caatgtgaca caccagagca gttattgatg
     3721 aatcctaaaa acgactacgt tcggcatttc tttgatgaac cgaaacagac gaaagagtgg
     3781 cgagtagaag atttagtcgt caatggatat ttcttaaatg aaataccaga aaatgctcgt
     3841 caacaagttt gctttgatac gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca
     3901 agcattgtga tagtagaaaa gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt
     3961 gcttttttaa gtcgagagga ggaaggaaat gaaaacatat gatgcaatca tcatcggttc
     4021 aggtgtgagc ggcttaagtg ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt
     4081 ggtcgaagaa gatctatggg gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt
     4141 cttgttaagt gcagtagagg cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa
     4201 cgagatccca actgccaact gggaagaact tcaaaaattc aaacgaacat ttacggatcc
     4261 agtcccagaa agccgaaaaa aacaattagc agaagctgaa atcgatcatc tttcaggaac
     4321 cgctcgtttt ttggatgatt catctattga agtcaacgaa gaagtcttcc atgcggatta
     4381 cttagttttg gcaaccgggc aaagacctac gatcttacca gtggagggaa aggaatactt
     4441 gaaaactagt gcagactttt tatcattgcc tgtactgcct aaagaaatca tttttatcgg
     4501 tggtggttat attgcttttg agctagctac gattgccaat gctgcaggga gcaaagtaac
     4561 cattgtccat cataatcaac ggccattaaa agaatttgag gcgtctttgg tagaggaagc
     4621 cgtccatcaa atggaagcat ctggcattca gtttgctttt ggtgttgaaa cccaaaagat
     4681 catttcagaa ggtactcgct atcgtttagt cggaaaagag acggaattag tagcagatat
     4741 gattttttgt gcgactggga gacagccgaa tacagaatct ttagcacttg agcaagcgaa
     4801 tatcgtcttt gataaacacg gaattgcagt caatgattat ctgcaaacga gcaatccaaa
     4861 aatttttgcg tgtggagata ttgtttcaag gaaaacccca aaattgacac cagtcgccac
     4921 ttttgaagga aattatgtag ctaagcggat aacggatgca acaagcgaac ctatcaaata
     4981 tccgattata cctacgatcg tctacgccag tcctaaactt gctgaagtag gagttacaaa
     5041 aagtcacgcc tcttcgtcag atcaagtggt agagatggat cttaccagtt ggttcaccta
     5101 tcatcgtgtc aatgaaccag ttgctaaggc tgaattgacc tttgaccagc aaaattattt
     5161 gattggtgct gcagtgatta gcgagcaagc agacgaattg atcgatgatt tgacactcgt
     5221 catcaatcaa aaattgacta aaaaagagtt ggatagctat atcatggggt atccgacatt
     5281 ggcaagtgat ctctcctatt tattgaaata atcagcatga taactaaagc gcttgttgct
     5341 ttttccttct tgtgctacac tttaaaaaat gaatacctgt ttctggggat gtcgtagacg
     5401 acatgagagt aagttgtgaa cttagaccca ttgaacctgt cagttaatac tggtgaaggg
     5461 agaaacatag cagacgcttg aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg
     5521 ttattcatat tagaagaaag gaggtaacaa aatgaagaaa aatctttatc cagcagtcag
     5581 tatggcattc attttaatta tttggcagat tgttgtcata gtttggaaaa tacctgcctt
     5641 tattttaccc ggacctgctg ctgtattttc tgcattagcg gcagatgcac aagtattact
     5701 ttcccattct gtcacgacat taaatgaagc agtgcttggt ttggtaatag ctagcgttct
     5761 ttcttttatt actgccttgg caatggatta ttggaagttt ttagaaatgt ctttttatcc
     5821 gttactagtg atttcacaaa tgttgccaat catggtgtta gggccattac ttaccctatg
     5881 gtttggtttc ggtatgcttc caaaagtgat ccttgttgtc ttgataagct attttccgat
     5941 cgtagtttct ttttcggata cattgaggaa agtatcaaaa gagcaaatca tctttttgaa
     6001 aacgatgggg gcagatacag ggaagattta tcgaatatat aaaataccag cgggtttatc
     6061 cggcttcttt tccggtttaa aagtggctgc tacttattgt gtcagcggag cagtcgttgg
     6121 agaatggcta agcgctcaag caggcttagg gtattacatg atacgcgtaa aaaacagcta
     6181 ccagatcgat aaagtttttg ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa
     6241 tggcgcttgt tcattattga aaaaagggta ttatcgtatg ctttatattt tttaagagaa
     6301 ggggatcaaa tgaaaaaatt atggctttta ttaccgctac tgttgctttt atccgcttgt
     6361 ggcactgcaa aagaaacaag tagcaaacaa gaaataaagg atttgaaaaa agtgacactt
     6421 gttttggatt atgtaccgaa tactaatcat actggcatct acctagcaaa agaaaaaggc
     6481 tattataaag aggctggttt aaatgtccaa atcatcgaac caggagataa ttcgacaagt
     6541 attggtctag taggtgcgga caaagcacaa tttggtgtaa gttatcaaga agatgtcact
     6601 tatgctcacg cagatggaca gaatatacca gtcaaagcaa ttgctacagt gatcaaacat
     6661 aatacttctg gattcgccac cctatcagac agtaacatcc attcaccaaa agattttgaa
     6721 ggaaaaacat atgcaggttg gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg
     6781 gaaaaagacg gcggggactt ttcaaaactg acaatggtag gaagcaatgg agaagggcca
     6841 gaaagcttag gaaaatcctc agatatccaa tggtactttg aaggttggga tatgatcaaa
     6901 gcaaaagaag cgggtattga actaaactat atcccattga aagaattaga tgaacggtta
     6961 gattactata ctccggtaat tattacgaat gatcaactga tcaaaagtga tccagaattg
     7021 gtacagtctt ttatggatgc cacgaaaaaa ggctatcagg aagctattaa ggatccaaat
     7081 gacagtgcta aactgttgca aaaatatgca aaagaaaatg atcggacgtt tttagaagaa
     7141 tcacaagcat tcttgtcaaa aaattatacg gatgatcctg agaattgggg attgatggaa
     7201 gaaaaagttt ggcgcaatta tacagctttt atgcaggaaa acggattgat caagcaagat
     7261 gtaccttcgc aagaactatt cactaatcag tttataaaat aggagtgata gatatgtcaa
     7321 tccaaatcag ccacttgaat gtagtcattg aaaacctgaa aatattagaa gacatccatt
     7381 tcactatccc taaagatacc tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa
     7441 cattgctgaa aactttaaca ggagtccagc ctatgacttc tggaacgatc aaagtagacg
     7501 atcaaaaagt aactggtctc catacagcat ttagttatat gccgcaagaa gatatgttac
     7561 ttccttggct gagcgtctat caaaacgtta cgttatatca aaaaatcaat catctaacaa
     7621 tcaatgaaga acaagtaagg gattatctgg atatctttgg gttaaacggc tatgaacatt
     7681 ttttgccgga acaattatct ggcgggatga agcagcgaac ggccttgtta cgaacaatca
     7741 tgaatccttc ttcttacttg cttcttgatg aaccatttgg agcgttagat gctatgacca
     7801 gagggcagat gcaagactgg atgttgaagc tgcctaaaaa agcaaagcgg actactttac
     7861 ttgtgacgca tgatatcgaa gaagcaattt atttatcaga caggattctt gtgctttccg
     7921 cccgtccggc acatgtgatt gctgagattc aagtgcctga aaaacgacgg tcacgtgaat
     7981 ggctactgca gcaatcagag ttgaagcaaa cgatttatca gttattggca ggtgaattag
     8041 atgttaagtg atgctcattg tcatttagat gggagtcaga gtttagcact ccttcagcag
     8101 gaacacacga ttttgacgat catcaattgt gattcgccag aagaatggaa ggaaaacaga
     8161 cagcttgcag caagtaagac tcaagcctta agttacggta tccatccttg gaaagcagat
     8221 agctatacgt ttgaacaagt tgagcctttt ttgaaaaaag caaggatcat cggtgaaata
     8281 gggttagata atatttggac gaatgtaccg atgactacac aaaaaaaagt atttgagcga
     8341 cagcttgctt ttgcagccat aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa
     8401 aaagagatct taaattatat ccaagcttac cctaatcact atttgatcca ttggtattct
     8461 tctttggact atcaacaaga ctatattgat ttaggctgtt atttcagcat tggtcttgat
     8521 ttgaaaaaga atccagcagt ctggcaacta gctaaagcag tcccaatcga ccacttactt
     8581 attgaaacgg atggactaca tgcagcagag tgggctttag gggggatttt ttcagaaaaa
     8641 gattatgtct cactgcttga agaacagtta aagacaatcg ctgagatcaa agagctatca
     8701 gttgaagaag ttaaaaaaaa tacttatcaa aacttggaac gtttcattag attaggtaaa
     8761 taaaaaagag atgtgacaca actagtgtcg cacctctttt tggctttctt tgtataaaag
     8821 taaactgttt gattaat
//
LOCUS       DAXWLF010000114         8788 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXWLF010000114 DAXWLF010000000
VERSION     DAXWLF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.114"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            11..310
                     /locus_tag="ACGE4U_001987"
     CDS             11..310
                     /locus_tag="ACGE4U_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394115.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(407..841)
                     /locus_tag="ACGE4U_001988"
     CDS             complement(407..841)
                     /locus_tag="ACGE4U_001988"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8394116.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1073..1783)
                     /locus_tag="ACGE4U_001989"
     CDS             complement(1073..1783)
                     /locus_tag="ACGE4U_001989"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFF8394117.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1773..2633)
                     /locus_tag="ACGE4U_001990"
     CDS             complement(1773..2633)
                     /locus_tag="ACGE4U_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFF8394118.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2638..3276)
                     /locus_tag="ACGE4U_001991"
     CDS             complement(2638..3276)
                     /locus_tag="ACGE4U_001991"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFF8394119.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3291..3590)
                     /locus_tag="ACGE4U_001992"
     CDS             complement(3291..3590)
                     /locus_tag="ACGE4U_001992"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8394120.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3633..5117)
                     /locus_tag="ACGE4U_001993"
     CDS             complement(3633..5117)
                     /locus_tag="ACGE4U_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFF8394121.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5137..5598)
                     /locus_tag="ACGE4U_001994"
     CDS             complement(5137..5598)
                     /locus_tag="ACGE4U_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8394122.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5616..6683)
                     /gene="ulaG"
                     /locus_tag="ACGE4U_001995"
     CDS             complement(5616..6683)
                     /gene="ulaG"
                     /locus_tag="ACGE4U_001995"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFF8394123.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6901..7671
                     /locus_tag="ACGE4U_001996"
     CDS             6901..7671
                     /locus_tag="ACGE4U_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8394124.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7944..8771)
                     /locus_tag="ACGE4U_001997"
     CDS             complement(7944..8771)
                     /locus_tag="ACGE4U_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394125.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2658 a         1853 c         1385 g         2892 t
ORIGIN      
        1 gaaataaaac atggattata ctgttgaatt tgacagtgaa ctacttaagc aatttccaca
       61 agaagaacat ttcatgtata aagaagcaat ttcccacaaa gtgacagagt atttggagca
      121 taattttttt cgtataaagc cagtgcgtac tcgttcggca aaaggaatat acgagatgaa
      181 agtcaaaatt aataaaaaaa attatcgcat agcatttgct ttaaaaaaaa atcatgtgtt
      241 cattttttat attagttcga atttgcaaaa aattgtattt gataaagagg tgagtaaaaa
      301 aataaaatag ctgagcattg gaggatagat tgagtgatac taagtctatc ctcttgtttt
      361 tctaaagaac taagctagaa ttttttcttt tgtacgttat tatatgttat ttgtcattac
      421 caaaaagcac cttttttctt ctagtatcta tgacataaga aataccaccc atttccttta
      481 cttgaacaaa tcctactgat tcataaaatt tttttgtttt ctcagtatca tctgtcaata
      541 attcgatttg gactggtcca taactagtga tcttctccaa agcttcattc aaaagcactt
      601 tgcctattcc ttggcgctga tacctcggat gaatgaggat atcttgaata aatgcgatcg
      661 tcgcaaaatc agtgatccaa cgaacgattc cgataatctt cccttcccca ttacgcttta
      721 tcagagattc agattgttca aatgctctag ctaaccttgc agtgtctttt gtgtaattcg
      781 tccaaccaac gctcttatat aattcagaaa tctcatcgat cataagggaa tcaatattca
      841 tttttttctt cctttcttat acaattctcc ctctaataat acagttcttt gactatttaa
      901 tctatcacat ataactctcc tgtaaagatt ctctcgattc cttaaaattc ctctttcctt
      961 ttagaatgca tactttttga taaataaaaa ggttgtgata aattttcttc acaacctctg
     1021 tttctctcta tcaagtgtta tcttttaatt ttctaccctt accctagcat ctctattgcc
     1081 cgtagtatgc atactttcca tgtttgcgaa aataatgttt atctagtaaa tattgcggga
     1141 tttcacatat attggggttg atcagctcgt tttctttggc catcagacaa atttcatcta
     1201 agatcaaact gttttctact gctttcatag gtgaatcccc ccatgtgaac ggtccatgtc
     1261 catagacaag tactccaggg acttcgtttg gatctaattt tctttcctta aacgtctcga
     1321 caatcacatt ccctgtatga acttcgtaag cttctcttac ttcttctttc gttaattggc
     1381 gtgtacaagg aaccttacca taaaaagcat cagcatgagt cgtgccataa gctggcaaat
     1441 cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg agtatgagta atagcagtta
     1501 tatcttcaaa agtttgatac aaaacaacat gtgtggggag atctgaagat ggttttaagc
     1561 tatcttcttc tattatattt cccgacaaat ctgttaccac catttgatct gcttgcatgc
     1621 attcatatct gacaccgctt ggcttaatga cgatgactcc tagctcacgg tccacttcac
     1681 tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg caatgccaga tttgcttcaa
     1741 ggacacgttg cttcattgct gcaatttttt cttcacaaga catcatagcc agcctccttc
     1801 aatttagaac ttaagtagtc ttttgcctgc tggatttctc ttcgaaagtc ttggcttttt
     1861 tcactccaca tttcaatcag aaatgttcca tcataatcta gacgtttcaa atgtcttaat
     1921 aatccaagaa aatcaacaca accgctacca aaaggaacat cacgaaactg gcctgaatat
     1981 tcttttgtta cagggtaagt atcttttaag tggatagcag tgatataatc gatccctttc
     2041 tcaagttctc gagccggatt attttccggc catgcggaca aattgcctag gtctggatat
     2101 acttgtagaa aaggtgaatg aatctgttct ttgatttcta aaaattttga aatggagttc
     2161 ataaatggat catccatgat ttccacggaa agaatgatgc catatttaga agcttccttt
     2221 acgctttctt ttattccttc gataaagtct tctctggaat tcattgattt ctcttcataa
     2281 taaacatcgt atccagctat ttggattact ttgacgctta gattatgcgc caaatggatt
     2341 gcttttttca ttagttgttt cgctgctttt ttcttttctg gatcagcaga accaaacgga
     2401 aatcgccgat gcgcacttaa acaaatcgaa tggatacgta caccggagct gaacatctct
     2461 tctctcagct cagctatctt ttcttcagac caatctaatc gtgctaaccg ctcatccgtc
     2521 tcatcaatcg acatctcgat aaaatcgaag tccatttctt ttacaaggga aaaacgttct
     2581 ttccatgaaa tatctttggg taacgctttt tcataaatcc caatttgagc catttcctta
     2641 cccccagata cgaatgatct catctttgaa gttttgtgcg gccttcatcg gatcatttgc
     2701 tgctgtaata ccacgaccag tgataaaggt atacacatcg atcccttcaa ataagtgtaa
     2761 tgtgtgtgga tttaatccac cagttacaga aacattaaag cctagttcaa tcagtttttt
     2821 gatcttcgat aaatcttttt caccccaagt ttcaccagat aacagcgcat cacggctttg
     2881 atggtagatt acctgccgga taccgatgtt atgccaatcc atggcttgtt caaaagtcca
     2941 attgccgtag agttctactt gcaattcacc tatttctttt tgtgctgcct ccatagttgg
     3001 aatcgttgca caacaaatga ctgtcatcca gtctgctcct gcttttgcaa cattactcgc
     3061 cactgttcct ccagcgtctg cacatttcgt atcagcgact agttttttat ctgggaacat
     3121 actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag caaagaattg tgcccacttc
     3181 aataatatct actacatcac caactttcat aacatctcct aatgcatgtt ctaagtcatt
     3241 gtgatctaac gcaatttgca aattcggcct gctcatctta gttccttcct ttacttgttt
     3301 ttttggtacc actttgtttc agcaaatttt tcactgattt ctttgactga catcacattt
     3361 ttgataccga taactgtaac tccttttttc tctgcctgag taaacatatt caaaaaattg
     3421 actggcgtaa aaactacatc gtattggcca gctgaacttt ttccctctga aatggcacaa
     3481 tgatggattt tggtaattgg tacgtttagt tctttcattg ctttttccac acttctcatc
     3541 atcatcaagc ttgttcctga tccatttgca cacgatacta aaattctcat cttaatccct
     3601 ccattatgag tgttgtcgaa tcagattttc ttttatactc tctgcttttc tgcatatgtg
     3661 tcatagtctt ctacaatcaa aaaataacca tcaggatttt tccgatattg taactgcgga
     3721 atagccaata aaccaatgac aactacggca attccagcat aacctaggac tttcatgatt
     3781 actgtaaaca atggccaagc tgttgcccag tcgaacatcc cgatataacc accgtatgtt
     3841 gacatcccaa tccatgtagc gaataatgct gaaccgccta cttgtatgat tcctgaaaga
     3901 aacgggaaca ggcaagcggc tttgaatcca cctcggttat tggcataaac tgctataacg
     3961 gcattatcaa agaacaacgg aatgaaaccg gctatgacga tcgttggtga tttcaataaa
     4021 atcagtaaac caatcatcaa aaactgtccc aaagcaccaa acaagaaccc aatcgttacc
     4081 gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa ttcctggaac agctcctggt
     4141 aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt catcgacgaa tgtccgaaca
     4201 cctaattgca agatcgccag atataccgca aaatttaaag aagtcgtcat aatgtagaat
     4261 aagaaactct gcccttcttg catgaattgc gcttgaatca aataatcttt gcctagtaca
     4321 atcagaatga tcccaaagaa aaatagcatg agtaacgaag tagcaaccat attctcattg
     4381 aaaatcgaca agaatcctgg taatttgata tcttcaattt ttttgtcttg cttgattgat
     4441 ctatttttct tttcatcccg ctttttcatc cactcagcta attttgcaaa tataaagatt
     4501 ccgaacattt gctgatgtgc cactgcaaat ccggcacctt ctgtcaattc ctgcgtgatt
     4561 cctactgtta agttagaagc tactgtccaa taacaaccta aaatcagtcc cataaaaatc
     4621 aatgcttgta tcctacctag atcaggaaag caaaacagta aaatccaaaa agcggtagct
     4681 gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac gcagtttcgt atatttctga
     4741 aaacggacta ataagatatt taaaacaaat gcaatcagta ataaaatcat cacatctcca
     4801 aaggtacggc caaatgtctc cattaatcca gcatccacag cattttgtcc aaaataagga
     4861 tctgtaacca tggcatccaa attgaaccgt tcctttagtc caaccaagat tggccgaaaa
     4921 ttattgacca aaccaccaga gccaacagtc aaaataaagt aaccgaccgt tgcctttaag
     4981 aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa tatagcctaa taacacgata
     5041 aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg caaaatacgt ccaaatgctt
     5101 aacaaaaatt cccccatctt ttttctcctc tatcaattaa ttatattctg ccatcacaac
     5161 ttggtaatca tcgatggagt tcgtagagat gagacgctca accaatccat ctgtcattaa
     5221 taattcggat aatttttgga tattttctag atgttcttct ggatcttttg ctgcgagtgt
     5281 aaaaaacaaa ctagccgttt tctcctcatt ctccccttca ctttcaaaac ggattggctg
     5341 tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca tcactttttt ctgaagaatg
     5401 cggcattgct acttgaggtg cgatcacaat ataaggaccg tatttttcta cacaatggat
     5461 gatctcctcc acatacgttt catcaataat ttccttatcg atcaatacaa gacagctttc
     5521 ggcgatagct tcacgccaat ccgtgatttc tttatctggg taatgaatca attgatggtc
     5581 aaaaaagtat ttcagcaaga aactcccctt ctttcttata aaaacgacgg aaacggtgta
     5641 tcattttcaa tcgagaagac atcttcaaat cctcgataaa aattaaactc aagatcatct
     5701 ttgtttgtcg gatagatgaa tttcccgcct acttgccaga tataaggttt aaatccatac
     5761 ttcaggcgat cctttttgaa tttccaaatt tcagtgatct cttttggatc agccataaaa
     5821 ttagaccaaa tatcgtagtg gacaggaatc actacttttg cattcaatga ctccgccatg
     5881 cgcagcatat caacagaagt aactttgtct gttatccctc gtgggttttc accataggcc
     5941 cctaaacaga catcgatttt gtgttcatta ccatgtttgg caaataaatt ggagtaatgt
     6001 gaatctcctg catgataaag attccctcct gatgtttcga ataaatagtt tactgcaatt
     6061 tgatccatct cctgaggcat ttttcctttt aatacctctt ctggatcttc acaagtgacc
     6121 aacgctgtcc gatcaaacgc ttctaaagct actacattga tgtctttgat tttgactgca
     6181 tcacctggct ttactgtaat cgttttttct tcaggaattc cccatttttg ccacgtgcga
     6241 accacttctt ttggcccaat gaatgttgct tcaggacaat tttgatgaac tgctgcggct
     6301 gtatggatat ctagatgatc ggaatggata tgtgtaacga ttaaagcgtc tacatttttt
     6361 atcgcaaaag gatcgattac gaaaggctga gtacgtaaat taggctgcat attttgaacg
     6421 ccactcatac gcatcatctg atggcctttt ttcatcatgc cattcccatg actacgtttt
     6481 cccgtcccac accatagatc acataaaata ttcgttcctt catgactttt cagccaaatt
     6541 cccgtacagc ccagccacca catagcaaaa gttccaggat ccactacttc tgtttcgatt
     6601 tcttcattca agtatgtgcc ccactcagga aatgtttcta ctaaccactt ttcttttgtt
     6661 acttcattga cagttttact catttgcttg ctccccccat atcgttgttc caacttacaa
     6721 gtaaatatta atgtgtatca gcatccatga aaaccctttt atatgaccgt ttgtttttta
     6781 tatgattttc tttcagtaat caatgattgt ttcagtataa taaagacaga tatgaatgtt
     6841 taaatgaaca actagtcaaa taaatccatg gatcaagatg tccctcagga ggcaaaagtc
     6901 atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag aaattttgga actgcttcag
     6961 cagactgaac aactatcaac tttcgaatta gctgaatccc ttgatgtatc tattagtacg
     7021 atacgaagag atttaaattt attagaagaa aaaaacgaga tcatacgcaa ttacggttat
     7081 tgttcttata attacaaaaa ccagactgat tttgatcaat ctggtcctga aaggattaaa
     7141 caagcgattg ccagagaagc tagtaaatac attaacgatt atgatacgct gtttatcaat
     7201 tctagctcga ctgcgttgaa aacactggat tttcttcaat ctaaccattt gacagtcatt
     7261 acaaacaacg tgaaaatcaa ttactctccg cacaaagata attgtagtta tattttaact
     7321 ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg acatcgcatt aaatacgatt
     7381 tcttcagtca atgcagacat ttgtatcatt ggctgtagcg gagtcgatgt agtacatggg
     7441 gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa tgatggtcaa caagacaatc
     7501 aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac tgacatcaaa gttcaagatt
     7561 gctgatattt cagtctttga ttatttgatc actgataaat tttgttctga aaaagtatta
     7621 gaggagatca gaaatacggg gataaaaacc attcaagtag attcatttta agaaccagga
     7681 cgtaaatatg taaataggat tatcaaaaaa atgaataaca aatttatcga taaatgaaaa
     7741 acacgctacg ctcaaattga taaaggaatc ccaagatgta tccatccaat cgataccatc
     7801 ataatctatt gcttctttta taaccgttat atgttgccta aataggaatc aaacaatatc
     7861 gaaaaaagga ggtatgacaa aagtcacacc tccttttttc gaataaacgg tattcaaatc
     7921 agaacactga tttcacaacc actttatttc aggtcgtttt caatcacctt taccgcgtaa
     7981 tcatttccgt tgaaacgcgc tatttttttt gcgtccatat atctttctaa ataatcatct
     8041 tctcctaatt gcttatggca agaagcaatt tcataaagca agtattccaa tgcataagtc
     8101 gatttatgtt tcttattata ctcaataccc ttctcacata attggattgc tttatcataa
     8161 tcctcgattt tacggtaata ggtggcgata tcataatata caatgcagac catcagatta
     8221 tcaaaatcag tctcactcaa tattttaagt gtacgctcca cataaacttt cgcaaaatct
     8281 aattctcctt ttgacaagta gatcattgcc acgtttgcag ctgcttgtaa gtgatggata
     8341 tctgtttcgg tcgtttgttc caacacttga ttaaaataaa ataatgctgc atctggtttt
     8401 ttcgcataaa gatgatcaag cagtccttga taaaataaaa tttgatttaa aagatcagaa
     8461 ttacttactt tttcctgatc gatcgtttgt aaaattttat aagattcttg gtattttgat
     8521 acagaattca atgattgaat ctcatttatt tttttcctga ttttgtcttc ttctgtttct
     8581 actaatatat cggagatctc gacattcagc tttttgcaaa gcgcacttaa gatattgatg
     8641 cctggcgaca tattcttgtt ttcgatattg ctgacagttg cttgttcaca aatgccttct
     8701 gctaattgta cttgtgttaa cccctttttc tttcggtatt cttttagtaa tgctccatct
     8761 actttaatca aaacaatttc cttccctt
//
LOCUS       DAXWLF010000115         8650 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXWLF010000115 DAXWLF010000000
VERSION     DAXWLF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.115"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(63..1106)
                     /locus_tag="ACGE4U_001998"
     CDS             complement(63..1106)
                     /locus_tag="ACGE4U_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFF8394126.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1483..2448)
                     /locus_tag="ACGE4U_001999"
     CDS             complement(1483..2448)
                     /locus_tag="ACGE4U_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFF8394127.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2682..3527
                     /locus_tag="ACGE4U_002000"
     CDS             2682..3527
                     /locus_tag="ACGE4U_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8394128.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3718..4905
                     /locus_tag="ACGE4U_002001"
     CDS             3718..4905
                     /locus_tag="ACGE4U_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8394129.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4911..5267
                     /locus_tag="ACGE4U_002002"
     CDS             4911..5267
                     /locus_tag="ACGE4U_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFF8394130.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5269..5607
                     /locus_tag="ACGE4U_002003"
     CDS             5269..5607
                     /locus_tag="ACGE4U_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFF8394131.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5683..5939
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6002..7036
                     /locus_tag="ACGE4U_002004"
     CDS             6002..7036
                     /locus_tag="ACGE4U_002004"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFF8394132.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7029..7715
                     /locus_tag="ACGE4U_002005"
     CDS             7029..7715
                     /locus_tag="ACGE4U_002005"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFF8394133.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7739..8587
                     /locus_tag="ACGE4U_002006"
     CDS             7739..8587
                     /locus_tag="ACGE4U_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8394134.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         2876 a         1577 c         1845 g         2352 t
ORIGIN      
        1 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
       61 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
      121 gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
      181 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
      241 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
      301 acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
      361 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
      421 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
      481 ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg
      541 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
      601 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
      661 gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
      721 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
      781 atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg
      841 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
      901 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
      961 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
     1021 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
     1081 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
     1141 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
     1201 aaagtcttgt acgacctctt gtttcgaata aacggtgttc cagaagtagc ttctacggaa
     1261 ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
     1321 gaagctgacc acttctttta cgacctcttg gtaaacggtg ttcaaaagct tgcatctgcg
     1381 gtaataagcc caaccagtac aaaaacatga acagctgttt ttgtttggcc gttcttatta
     1441 cttgatgcag aacagctttt tcacaaccta ttgaatcgat tattaagcga tttttttaga
     1501 aacaaatttt ccatgtttgc tatgttcttt aattaagaaa cgtacgtaat gagcaagaac
     1561 gtagaagaca actacacttg caaacagaca ttccgcaact tttaagaaat ccaatacgct
     1621 actatagata cctaaactgt tgaagtaatg aacagtagta aatatagctg ttgcagagat
     1681 aacaagcggg aatgtaaagg ctgcatagct tggataaaaa cgaagcttga acaaacgtgg
     1741 aagcatagac agcacaacga aatacaatac ttgagataaa gcgaacaatg ctacgacaag
     1801 ataaaaatta ggttcagcaa agtttttaag ataacctgtt aggcaaagag aaccaggtgc
     1861 tgcaacgatc gtaatcagcg gacgtgtcgg atgagccata tttcttacga aaaagacacg
     1921 tttgatgatg atcggcaata agaacgcata gcagatcaat gaaatccaaa agaaaatttt
     1981 tccaataagt gggaagaagt tcccggcagt tacagtgatg accccaaatc caacgtaaat
     2041 gataaaccag cttggataaa ttgcttcaat tgaaagatca gctttgataa catggtgata
     2101 attgaaataa aagacaagga tcacttggaa gataacagcg cacagccaaa tgaatttgac
     2161 aattggtgca atactttcat atgaaacaaa ataagtacaa ataaccatga gtgacatcgt
     2221 aaaagttggt gaaacggagg cgatgattgg atcatgcaac gtttgtttcg tgtgttcgaa
     2281 taagataaac aatttaacaa aaatgaatat cataagaatc atagagatac cgccagttag
     2341 attgcctaaa gcaacatggt gatagtcttt aaacagattt ccaagggaag ctaacccaag
     2401 gatcaacccg cagatcggga tcggaataat tttcaaaaag ttcttcatat ataatattct
     2461 cctttttatt taatagattt tagtaccaag atgaacgaat acattgtaaa atgaatagga
     2521 atgtatgatt acgagcaagc agacagctga ttgtcttcat ctaagttact agttggatca
     2581 gcaactgttt tgttgtacat ttctattata gatggttttt tgtatattta aatcaaatag
     2641 tttttatagt aattatcata aaaagttatg gaagtggtgc catgtttaag ttattaacga
     2701 cttttcgagc agtatatgaa acaagaaatt tttccagagc agcagaagta ctgtttttat
     2761 ctcaaccagc agtctcgaat caaatcaaac agttggagcg ggaattgaat atcgatcttt
     2821 ttcaacgtaa cggaagaaaa gagatgcttc caactaagca agccgactta ttatatgaac
     2881 ggacactggt tttgttagaa gaatgggaag aaactcagca aaaattgctc aatgaacaaa
     2941 atgaagaaga agtatgtcgg atcgttgctt cccacacctt tgctgtctat cttttgcctc
     3001 agttgatgaa acatctgatc aaaaggtttc ctaaagtgca ttttgagatc gaaatcacga
     3061 attctcatga atcattagaa caagtagcga aacatgaatc cgattttggt tttatcgaaa
     3121 agccattaga gacttccggc gtacaaagat actctttgat ggaagaccag ctagtcatcg
     3181 ctggggaccc aaaaagcaaa ttgtggcttg taagagaagc aaactctggt gtttttcatt
     3241 acacgcagcg ttattttgaa gagatgaata tccaagataa gaagatgatc gtgaaaagta
     3301 acgatgtaat tgttgcgttg ctgaaggaag gaatcggtcg ctcgattttg tctaagcgct
     3361 ctgttccaga aaatgtgcca atgactccgt tgaatttcca tcgatatttt tatttcatcc
     3421 aacgaaccaa tctggtttct gatcagctgc tagagattgc cgaagagatc cttcgataca
     3481 atgaaagcac agaaacttct cacagcgagg agaatctcag ctggtagata attgcatgta
     3541 tcaaaaaaag ttctatgata cactaaagat aagcagtagg cttttgtact gcatttgctc
     3601 aagcaactgc acaaacggaa agttaatttt tcccaaatgt ctatggaaag actttccatt
     3661 atctaactgg accgctataa taatcagcag tatggttttt aagaaaaggg atgaaacatg
     3721 gaaaaaaaat taaaagcttc gaacgataat cgtattgatt atggcgtgat tttaccagtc
     3781 tttctgttat gtttgattgg tctgctctca ttatatgtag cacttagcca tgatccgaac
     3841 aatccagcag tcttgaaagg cgtagggatg caagttgtct ggtatttagt tggagcggca
     3901 gcaatcgttg tcatcatgca tttgaattcc aaatggatct ggaaactgac tccttacctt
     3961 tatggtttgg ggttagtggt aatgggactc ttgctcaaat tctatgatca gaatttagct
     4021 gcttcaacag ggtctaaaaa ctggtttcga ttcggtgcgt ttactttcca gccagcagag
     4081 ttgatgaaga ttgcgtacat cttgatgatg gcactgatcg taactaaaca taatacgcaa
     4141 gtaaaagaac ggacgatgaa atcggacttt tggctgattg ggaagctctt gatcgtgaca
     4201 ataccggttt tagcattgat catggcccaa gatgactttg gaacgatgct tgtctttctt
     4261 gcgatctttg gcgggatttt cctgatgtct ggaatctctt ggcgcatcat cgcacctgta
     4321 gtaatcttgg cagtagtcgt tggagcagga acgatcttct tggttacgac tgaaggcgga
     4381 agagacttgc tttataaagt tggattcaaa tcttatcagt ttgcacgtat tgattcatgg
     4441 ctggaccctt tccatgatac gtcaggaatg agttatcagc cggcccaagg acttttggca
     4501 attggtaccg gtggcttgtt tggtaaagga ttcaacgtaa gcaatatcta tgtgcctgtt
     4561 cgtgagtccg atatgatttt taccgttatc ggtgaaaact tcggctttat cggtggtgca
     4621 ttcgtcattt tcttgtattt tattttaatc tatcgtatga ttcgggtgtg tttcgatacg
     4681 aataatgaat tttacgccta tatcgcaagc ggtttgatca tgatgctgct gttccatgtc
     4741 tttgagaata ttggagcaaa tatcggatta cttcctttaa cagggatccc attgcctttc
     4801 attagtcagg gaggatcctc gattttaggg aacatgatcg gtatcgggtt gatattatcg
     4861 atgagatacc aaaatgaagc acctgcaaga tcttcaagga ggtaagaaaa ttgtacacct
     4921 tttactggta cccgaaatgt tcaacatgca aaaaagccaa agcgtggtta gaccagcatg
     4981 gcgttagtta tcaaaccgtt gatatgatca aagaagtacc gaaggcctca acgattgaga
     5041 cttggctaga aacgagtcaa gtaccaatcc gtcgcctgtt caacacaagc gggatgaaat
     5101 atcgggaact tggactgaaa gatcaagtag gcgagtttac ggcttcaaaa gcgagccaag
     5161 tcttatcgac agatggtatg ctgatcaagc gaccactgat cttacgagag gaccagcttg
     5221 ttgcaattgg attcaatgaa aaaatttatg aaggagaact gatataaaat ggcaggcaag
     5281 catttgaaga aaaaggacaa cctttggatc ttatttaatg gaaaagaata ctgtgttggt
     5341 ttgacaaatg aagcgcaaga agaacttgga gacatcacgt ttgccaatct gccaaaagca
     5401 ggacaatcgc taaaaaaaga tgaacctctg atcgaagttg aagcggaaaa agcggtcaat
     5461 gaatttgcca gcccgctaac gggtacagtt tcttctgtta atgataaaat cggtgaagat
     5521 atcaatatct tgaatgattc agatgaaatg aatgcgtgga tcttaagttt caaagatgtt
     5581 gatccaaaag aatttgacga attataaaat cagaaaatca cttgaaaaaa gttgacactg
     5641 attcttccag ttgctataat cataagcaat cctatatgaa acgagaaagc tttgaaaaga
     5701 agagtacatg tcacggacgt ttgaagagag ccctgtttgc tgaaaatggg taacgaaaat
     5761 gagatggaag atggtcttag agcagagtaa gcgagcgtaa gcaaacttac tacgggagtg
     5821 cccgttacag cgcgacagta tacggaaaga agctaagtcc aagtactgga agaggctgtt
     5881 attgcgaagt aacggtaaat caaggtggta acacggaagt tcaagctttc gtccttttgc
     5941 ttaaggcaag agggcgaagg cttttttgtt tgaaaaatca taagagaaag atgtgaatgg
     6001 catgtcatta atccaactaa cgaatgtcaa aaagacattc aatgggaaaa acggaaacat
     6061 ccaagccgtc aatgacgttt cactaaacgt tgaaaaaggg gatatttatg gaatcgtcgg
     6121 ctattctggc gcaggaaaaa gtacattagt ccgtctcctg aatggacttg agcttcccac
     6181 gagcggcgaa gtgatcgtaa acgatcaaga tattacaaaa ttaaaaaaca aagaattacg
     6241 tacattccga aagaaaatcg gtatgatctt ccagcatttc aacctgttat ggtcaagaac
     6301 tgtacttgaa aatatccagc tgccgctgga attagctggc gtaccaaaga gcaaacgtaa
     6361 agaacgggca gaagaactgt tgcgtttagt cgggttagaa ggacgcggac aagcatatcc
     6421 ttcacaactt tccggcggac agaaacaacg cgtcggtatc gcacgagcat tggcaaatga
     6481 tcctgagatc ctgctttgtg atgaggctac tagcgcattg gacccgcaaa caacagaaga
     6541 agtattagac ttattgttag cgatcaataa gaaattgaat ttgaccatcg tcttgatcac
     6601 acatgaaatg aatgtgatcc gcaaaatctg caacaaagtc gctgtgatgg agttggggaa
     6661 agtcgtagaa gaaggagacg tattgactgt cttcagacaa ccgaaaaaag aagtaaccaa
     6721 acgctttgta caacaagaca tcgaaccaga agctgattcg acagaactat tagctgaact
     6781 catcaaagaa aatccaagcg gccgtttggc aactttgacc tttagcgaaa gaaatgccaa
     6841 cgagccagtc atttcccaag cgattcgacg ctataacatc gatgtcaacg ttgtgtatgg
     6901 aaagatcaaa caagtgaaag aaggttcctt tggttcattg actgtcttga tgactggtga
     6961 aaccaaagaa ttagatcaag cagaagtgtt catcaaagaa caaggagtag gaattgaggt
     7021 gatccatcgt ggataaaagc ttttcagaaa catatctaga ttttaaacaa atcaacccgg
     7081 aaacgatgaa tcaggctatc cttgatacac tatatatgac attaatctca atggtcttag
     7141 tgacgatcat cggattagtt ttgggattga tcctttactc tattggacgc aaaaacaaac
     7201 cgtatgctcg aatcatttat ggaatcgtat cgatcatcag taatattttc cggtcaacgc
     7261 catttatgat cctgatggtt ttgatcattc catttactaa agcaatcgta ggcactatgc
     7321 taggtgcgca agcagccatt ccagctcttg ttttatccgc agcaccattt tatgcccgtc
     7381 tagtagagat tgcttttcgt gaagtcagcc ctggtgtact ggaagcagca gatgcaatgg
     7441 gagcaagtta ctgggaaatc atctggaagg tcttgatacc tgaaagtatt cccgcattga
     7501 tttctggatt aacagttaca acgatctcga tgatcggatt tactgcgatg gccggcgcga
     7561 tcggcgcagg tggacttgga ggtcttgctt ggcaggaagg ctaccagcgt ggtaatctaa
     7621 cagtgacatt cgttgcgact ttaattattt tactaatcgt atttatcgtt caaggaatcg
     7681 gtgatttctt aactaagaaa acagacaaaa gataatagga tataggggga tgtaggatat
     7741 gaaaaagaaa atttttggat ttgcagcagc gcttttgctg actgtaggat tagcagcatg
     7801 cggtaacaac agcgacagca aagattcagc aaataaagca gacgacacga cattgaaagt
     7861 cggcgcttca ccgacaccgc acgcagagat tttggaacat gtgaaaccat tattgaaaga
     7921 tgaaggaatc gatcttgaaa tcgtcaaatt cgatgactat gtattaccga accaatcttt
     7981 ggaagaaggc gacattgatg ccaattactt ccagcatatt ccttatttga acaaagaaat
     8041 caaagaaaaa ggatatgact ttgtcaatgc cggtgctgtc cacatcgaac caatgggctt
     8101 gtactctaaa aaagtcaaag atgtttccga attaaaagat ggtgcaacaa tcattacttc
     8161 aaattctgaa tcagactggg gacgcatcat cacaattcta caagatgctg gcttagtcaa
     8221 agtcaaagat ggagtagatc tggaaacagc aacattcgat gatattgaag aaaaccctaa
     8281 gaacttgaag ttcaaccata cgatcgaccc atcactttta gcttctacgt accaaaatga
     8341 tgaaggtgac ctagttgcaa tcaacgcaaa cttcgcttac ggcgcagggt tgaatccatt
     8401 agaagatgcc gtattgcttg aaaaggacaa ctctccttac gtgaatatta ttgcgactcg
     8461 taaaggtgat gaagacagtg acaaaatcaa aaaactgatt gaagtgcttc accgcaaaga
     8521 agtccaagaa tggatcacag acaaatgggg cggctctgtt aaaccagtaa aggctgatgc
     8581 aaaataaggt atggaacaaa aaagatgaga aataagtctg taatggctta tttctcatct
     8641 tttttatacc
//
LOCUS       DAXWLF010000116         8408 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXWLF010000116 DAXWLF010000000
VERSION     DAXWLF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.116"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            254..733
                     /locus_tag="ACGE4U_002007"
     CDS             254..733
                     /locus_tag="ACGE4U_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFF8394135.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="ACGE4U_002008"
     CDS             766..2994
                     /locus_tag="ACGE4U_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8394136.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="ACGE4U_002009"
     CDS             3123..4766
                     /locus_tag="ACGE4U_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFF8394137.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="ACGE4U_002010"
     CDS             complement(4839..5384)
                     /locus_tag="ACGE4U_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFF8394138.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="ACGE4U_002011"
     CDS             complement(5407..5892)
                     /locus_tag="ACGE4U_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8394139.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="ACGE4U_002012"
     CDS             complement(5882..6694)
                     /locus_tag="ACGE4U_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFF8394140.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="ACGE4U_002013"
     CDS             complement(6833..8365)
                     /locus_tag="ACGE4U_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFF8394141.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
BASE COUNT         2843 a         1599 c         1654 g         2312 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaaga
//
LOCUS       DAXWLF010000117         8378 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXWLF010000117 DAXWLF010000000
VERSION     DAXWLF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.117"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            208..2250
                     /locus_tag="ACGE4U_002014"
     CDS             208..2250
                     /locus_tag="ACGE4U_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8394142.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2261..2881
                     /locus_tag="ACGE4U_002015"
     CDS             2261..2881
                     /locus_tag="ACGE4U_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFF8394143.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2893..3351
                     /locus_tag="ACGE4U_002016"
     CDS             2893..3351
                     /locus_tag="ACGE4U_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8394144.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3370..3648
                     /locus_tag="ACGE4U_002017"
     CDS             3370..3648
                     /locus_tag="ACGE4U_002017"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8394145.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3673..5040
                     /locus_tag="ACGE4U_002018"
     CDS             3673..5040
                     /locus_tag="ACGE4U_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFF8394146.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5055..6200
                     /gene="dgoD"
                     /locus_tag="ACGE4U_002019"
     CDS             5055..6200
                     /gene="dgoD"
                     /locus_tag="ACGE4U_002019"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFF8394147.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6227..6718
                     /locus_tag="ACGE4U_002020"
     CDS             6227..6718
                     /locus_tag="ACGE4U_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394148.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7161..8360
                     /locus_tag="ACGE4U_002021"
     CDS             7161..8360
                     /locus_tag="ACGE4U_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8394149.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
BASE COUNT         2574 a         1384 c         1805 g         2615 t
ORIGIN      
        1 catccaatat tctaccgaat atttaatatt cacattagcc ctaaaataat tgtcatacca
       61 tgttacattt gatataacag cgttcatttt aaaaatagct gttagtgtga caaaacatta
      121 aaccaagtgt gacaaaaaac gtgatgaaag ctttccggaa acgtatgtga acaagtatat
      181 aatgattttg tcaggaggat agtcaaaatg aataaacgaa ttcgtgaaat actcaaactg
      241 attattaaaa accctgaaat gaaactttcc actttaacaa gtgaacttga tttgacacgt
      301 cgtcaaatca attacgcgat caatcaattt aatgaagaat tgaaaacaaa gaacattccg
      361 ttaattcaga gaagtcattc tggaaatatt acggtaccaa tcgaggttat ccaaatgata
      421 tcacagcctg attacgacat aggtgatgat caggaacttg tcgttctcac tgatagtgaa
      481 agacaagcat tgatagtgat gactctgatc gctaatatcg attacatcag tctggatcat
      541 ttggtagatg cggttgatgt tagtaagacg actgttatgg aagatatcaa acgaacggaa
      601 ccgttcatca gaaattatag tctgacaatt cgatataatc ggatgaatgg ttaccaattg
      661 gttggttcgg aacaccgtat cttacagctg ttatcggatc tagtaaaacg gtttccgatt
      721 tttcgccgag aaatgattcg cagaaaattg gcacaagatg tttctgaaga agaaatcgtc
      781 catttgatcc ataacatgga acaaatgttg catcttagtt attctgatga gtcggtagac
      841 tatttgcaga catcagcgag atttttagta agccgtggga tcaagatgtc agataagcac
      901 gcgttttttc ccgatgatgt gacggatact cctgaattcc gaatgctgat gatcttggta
      961 catgaaacag gttggagtct atccaaaagt tatatggagt ggttcacgtt attattttta
     1021 acttccaata tttttgagag caaaactact caaaattatg attcggatca agaattgcga
     1081 aaattgatca gccaaatggt tgaaaatttc caaaatcaaa cgttgatttt gatcgatgac
     1141 cgagaagact ttgaacgtag gatactgagt catctgcgtc cagcctgttt tcgaatacgc
     1201 tataacttga gtttaggagt ttattcaata gatagtctga tcaaggatag taatcatgcg
     1261 attttgattg atttgatgaa agaattgatt attcctattg agaattggct aaataaggcc
     1321 tttccaaatg atgaattaga tcttttatcc tattactttg gctatcaatt aagtaatcat
     1381 aataaattga ataagcaacg acaaagggct gtagttgttt gtactaatgg tgtcatggtc
     1441 tcaaagttag tacgagccaa tatggaaaaa ctttttccag agatccattt tttagcttcc
     1501 ctatctgtgc gtgatttcta caaatttgaa gctgattatg atctggtctt tactacgacg
     1561 ccactaaaaa gttctatgct gcagtttatg atcgagccaa tcatgacata tcagcaacaa
     1621 atcagtttac gatatcgagt tttgagtgat ttaggaatta ctaaggttga tcgagcaatg
     1681 gatgaattgt taacaatcat taggaaatat gcaaatatca ccaatccagt ttcgttgcga
     1741 gaagaactgc agtacttttt gatcaaggaa gatggaagca cgccgctgga caacttcaat
     1801 gtattgcctt cattgactta ttacttgcgg ccgagttatg tacaaatcgt tgaagaagag
     1861 atgtcttggg aacgagcagt ggaggtggct tgccgaccgt tattggaaca tcaaatcatt
     1921 gactcgcgtt ttatcgctga ttgcttggat caaatcagac aaccgggata tgcaggttac
     1981 ttgggaatga agacttgcat tccccataca acggtcgata aaggtgtaat cagtgacggt
     2041 gtcagcattt tagtttgtaa aaagccgata cgatttccta atggtgaaga tgtcagcttg
     2101 attttgccgc tttcattttt tgatttaacg aagcatctgc gagccatcaa tcaaatcgct
     2161 gatatcgcca aagatgattc attgattgag cagttattga ttgctgatga cgaaaagaca
     2221 gtttatcaac tattaagaca aagatcttaa ggaggaaaac atgagactag cagattatcc
     2281 taaattgaca gtgatcatgc gtggttatac ttttgatcag gcagatgcta ttttacaagc
     2341 aatgcaggaa tttgaccatc aatttgccgt agagatgaca atgaacacaa aaggagctct
     2401 tgaaaatata catgaactaa atcaacgtta tggtgacacg acttatatcg gcgctgggac
     2461 ggtaaggaca ttagaacaag cccaagcctg ctatgaggct ggtgcaaaat ttttattagg
     2521 accacatatg ttcacaaaag aaatgttgtc ttacgccaat aagaaaggat tattggcggt
     2581 acctgccgca atgactcctt cagaagtcga ccgtatgttt aaagaaggag ctgatattgt
     2641 aaaagtattt cctgcagcgg ttgttactcc acgttttttc aaggatattc aagcaccttt
     2701 aggtaagtta ccactaatgg gggttggtgg aatcagtaag gagaatgcga aagagttttt
     2761 tgaacaaggt gctagctatt taggtcttgg ttccggtatg ttcaacaagc aagacatcga
     2821 agagctaaat gttaaaaact tagcacgttc gatgaaagaa ttaattgagg ctattaaata
     2881 ggagtgagga ctttggaact ttatttgaat gaggaactcg tttttcgaaa tgttcaagca
     2941 aaaacagata gtgaagtctt agcgttttta gcaagcgaga tgtacaaaaa aaactacgtg
     3001 aaggaggaat atattcaagc aatccaagaa cgggaaaaag aataccctac tggattacca
     3061 tcaacaccac caggaatcgc aattccccat gcaaactatg aaatggtcaa taaaacaact
     3121 ttagcaattg cgactcttaa agatccggtc ctatttcata atatggaaga taacaatgag
     3181 cagttaccaa tccaaattgt gatcatgatg gcgattgggg aaccacatgg gcaagttgaa
     3241 atgttacaaa aaattgttgg aatcatacaa gatgagccac tgcggcaaga aatgatcaga
     3301 gcaggcaatg atactgaatt attagaacta ttaaagaaag cggtttttta atataaggag
     3361 gaaatcataa tgaaacgaat tttagtagca tgcggaaatg gtatcgccac ttcaacagta
     3421 gtagcaacaa aagttagaga gtatttgcaa caacatggca ttgatgtttc aacgactcaa
     3481 acaaaattga tggaagttcc aggaaaagtt caagactatg atctattggt gacgactggt
     3541 caatttgatg gtcaaacagg tggtgtacca gtaatcaaag ggatgccgat tttaacaggt
     3601 atcggtgccg atcagacaat ggaagagatt ttgaacttac tgaaataata gctgatcaaa
     3661 ggagctgaaa aaatgaaaat cattatgaac gtctttaact tctttattga tgctggacca
     3721 acagttatgt tgccagtcat catcacgatt attggtttga tttttgggtt aaagatttct
     3781 cgtgctttta aatcaggttt gacattaggt atcggttttg ccgggatcaa gttgatattg
     3841 gactttatga cgaccaatgt tggaccagct gccaaggcga tggttgatcg aactggtgtt
     3901 aaactagatg ctttggatgt tggctggggt tcgattgcgg ctgttacttg ggcatcgcca
     3961 atcattccaa tcttgatttt tgccattcta ttggtcaata tcgttttgct gattttgaaa
     4021 cgtacacata cgctggatgt tgatatctgg aactatcacc atatggcaat cgttggtgtt
     4081 atagtgtatt ttgtgactaa gaatgtcttc ttaggtgtag gtgcatcagt agtgatggca
     4141 attgcgactt ttaagatttc agactggtcg caaccgatgg tagaaagttt cttcggaatc
     4201 cctggtgttt ctttaccaac tgtttctgcg ctttcatcac tggttattgc ttggccgtta
     4261 aattggctgc tggatcgtat cccgttattc agaaaatcaa aattcaccat caaagatgca
     4321 caaaaatatt tgggattttt tggtgattct atgatcatgg ggttagttat tgggatggtg
     4381 attggtgttt tagccggcta tgatatcaaa gcggttttac aattaggtgt cagcatgtca
     4441 gctgttttgg ttttgattcc taagatgact tcattattta tggaaggatt gatgccaatt
     4501 tcagatgctg cacaaaaatg gtcacaaaaa aaattcaaag gtacacgttt atttatcgga
     4561 ttggatgctg cagttgttgt aggtaatcca gatgttatca caacagcatt gatcattatt
     4621 ccactaacga ttgcattagc tttagtattg ccaggaaacc gtgtgttacc attcgcagat
     4681 ttagcggttg ttcctttccg cgtggcaatg gtcgttgcct tgactcgtgg taatttatta
     4741 aaaaatatca ttattggctt agttgtgaca gcttcattgc tatggtgtgg gtcagctact
     4801 gcgccaatgc tgacagcaat tgccaaatcg gttggtatta acttaggaac ttcttcaatg
     4861 ttaatctcat catttgctgc gacttccatg atccaaagtt atttggtatt tatcgcattt
     4921 gcttacaaac caatcatcgg tattccagtg ttgttattag cctttgcggg cgcatggtat
     4981 tacttcgatg gtatcaaaaa agttaatcat gaaatggatg aaccattgaa ggcacaatag
     5041 taggagggtt taaattgaaa atttcagatg tcacagtgta taaagtaaaa ccaagatgga
     5101 tttttgttaa gatctcgact gacgaaggaa tcgaaggctg gggagaaatg atctcaggaa
     5161 ctaaaacaga aacggttgtt gctggagctt atgagatagg aaaccggctg attggacgta
     5221 atccgtttga aatcgagcgc ctcttccaag aaatgcatcg atctttcttc cgaggcggtc
     5281 ctattaatgg aaccatcgtt tcgggtttag aaatggctct gtgggatatc aaaggaaaag
     5341 cgtttaatgt tccggtttat gaattattgg gcggtgcagc acgtgataaa atcaaggttt
     5401 attcttggat tggtggagat cgaccaagtg aagttgcaga acaagcacaa gatcggtttg
     5461 atcggggctt tacggccgtc aagatgaacg cgacagaaga gctgcattat atcgattcct
     5521 accaaaaagt tgatgaagtg gtggaacgag ttgcgtctat tcgcgaacgt tttggtgatg
     5581 caatggatat tggtgttgat tttcatggac gggtccataa accgatggca aaagtgctag
     5641 caaaagcttt ggagccattc catccaatgt ttttggaaga agtcgtttta cctgaaaatg
     5701 aagaacactt taaagaagta gctgattctg tggctgtacc attagctact ggtgaacgct
     5761 tgtacactcg ttggcaattc aagaacctct ttaaacaagg tactgttgat attatccaac
     5821 cagatgtggc tctttgtggg ggaatcttgg aaacccgtaa aatcgcagcg atggcggaag
     5881 cttacgacat ggctgttgcg cctcatgcgc catatggtcc tgttgcacta gcagcaactc
     5941 tgcaagtaga ctcatgtacg ccaaacgtct ttatccaaga acaaagctta gggatccact
     6001 ataacaaagg ttttgatctg ttagattttg tgaagaacaa agaagttttc caatataaag
     6061 acggctacgt agatctacct tcaaaaccag gtctgggctt ggaaatggat gaagacaaaa
     6121 tcaaagaaat tgcacaagaa ggtttgattt ggaccaatcc gcaatggaaa aattatgacg
     6181 gtacgatcgc tgagtggtga taaatgaaaa taaaggatga tgaaagatgg cagaagcact
     6241 tatgatcaca gtttttttag gactgctgat tattttgcaa ataatcgatc aaaagaaaat
     6301 tatcgtgttt gctcagagta gtcctgtaag gaagagtttg agtattttac tgacagtcgc
     6361 catcctgttg attttctggc cggaacatct gactgatcag attaaactgg ttgcttttgc
     6421 ttctgtcatt ttgatcaatg gttttttgaa ggagggtcta gcaaaagatt ctgttgttag
     6481 atttggagta ttgaacggta actttaatcg atatcctcaa gtccaagtag aaaaactagg
     6541 tgacgataaa tcatttattt cattttacaa aagcaagaat aattcttttt caatgatttt
     6601 tgataaagat caaactgaaa tgatggatgt ctttcaagaa cttgatatcc attccaaaat
     6661 tactttagat caaagttcgg aagaaaaaag agcagttgta aaaaaagata atgaatgatt
     6721 ctactaccga aattatgatt tctttatcac gaataatgaa acttgtttta atgaatcata
     6781 ttgattgaag tatacaatgt atattaagtt ttggaaagaa atatattggt acaagatata
     6841 gcaggaatcg attactcgat ccctgctttt tttaacttcc tttgcagcaa aaaaagctcg
     6901 taatctaaga gatgatggat agtttttaga atatatatat tttcttcaat tccagaaaaa
     6961 aatgaccaag ctagtatgct aaaaaagtac gatgcttcta tgatcttttt tccgtcttta
     7021 ttaacagatg ttttaattga tcaacgcata ttgtaatcta ccctaatttc catagttctt
     7081 caccactttt aaaaatagta aaacccctac aagtatgcta tagtaaattg ctaaagaaaa
     7141 accaataagg tgggagatgt gtgcgcgatc aaacaagcgg aaaaatgatt cgtttttatt
     7201 atacaagttt gcattttctt tattggatga tgttttgttc tgtctatggg tttgcgacat
     7261 tgtttttgat ttatttcaag attgatccag ctaaaatcgg agggatctta gcattggtca
     7321 atattgtttc aacggtgctt cagccttttg tcagtcaagt gatcatcaag aaaagaaaga
     7381 tcccactgat caccgtgctg aagacgatga tagcaggatt aggagtgatt ctcttagctg
     7441 gggtattgat tccgaatttg ccgatcatta cttatatctt gggtgggatt ttagtagttt
     7501 ctatgcaatc atttctcaat gcgctggcat ttgagtattc aaataccggc tacaggatca
     7561 actttggctt ttcaagagct ggtggttcat tttcttatgc tgtaacttct tttttactgg
     7621 gacagctgtt agcgatttat tctgccgcga ttttgccaat cttggttata gttgaagcag
     7681 tgatattttt cgttattttg catctgctgc cggatgtaaa aaatcaagaa gtttttccta
     7741 tgacaaaaga acaggatcgc tcagtttctt tgagaaaaaa atatcctttc ctgattttgt
     7801 tatttttagg tttcagtttt ctttttactt tccacaccat gatcaacagc tttttggcac
     7861 agatctttga aaatatccac ggcggcagta aagaagttgg catcgcattg atgatcgccg
     7921 ctttatgcga attaccggga atgatctttt ttgaacagct tgtcaaaaag aaatccagca
     7981 aattttggct gtctgttgcc agtatttcat ttgctgtcag aggattcatg atgctgctgg
     8041 ccagttcgat cctgatgatg gaaatgacgc atctcctaca agccttcagt ttcgctttgt
     8101 ttattccagc ttcggcttat ttgttcaatc aatttctggc ggatgatgat cgtgtatttg
     8161 gacaaacgat gatcatcatc gccacaactc ttggtggggt gataggaaat gtcttgggcg
     8221 gcagtctgct gcagcatttc ggtgtatggg agatgctgat tttcggtact ggttttgcgg
     8281 tgctaggtgc tttattaact attttgggca ttcgaaaaat accgaagaca ggaaacgata
     8341 gtgattctct tgttgagtag cataaaaata cttagata
//
LOCUS       DAXWLF010000118         8333 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXWLF010000118 DAXWLF010000000
VERSION     DAXWLF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.118"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(84..761)
                     /gene="nth"
                     /locus_tag="ACGE4U_002022"
     CDS             complement(84..761)
                     /gene="nth"
                     /locus_tag="ACGE4U_002022"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFF8394150.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(768..1466)
                     /locus_tag="ACGE4U_002023"
     CDS             complement(768..1466)
                     /locus_tag="ACGE4U_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFF8394151.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1734..2918
                     /locus_tag="ACGE4U_002024"
     CDS             1734..2918
                     /locus_tag="ACGE4U_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFF8394152.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2924..3490)
                     /locus_tag="ACGE4U_002025"
     CDS             complement(2924..3490)
                     /locus_tag="ACGE4U_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8394153.1"
                     /translation="MTSFVNPFFSWFQIGFSYKIFLSLLNRKQKNNIMKEKEGVEMDK
                     RQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHIGKIRDPNVTKLANASYM
                     TRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKIRNRHEQLHQKWVQRDAT
                     VFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3593..3880)
                     /locus_tag="ACGE4U_002026"
     CDS             complement(3593..3880)
                     /locus_tag="ACGE4U_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFF8394154.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4051..5403)
                     /locus_tag="ACGE4U_002027"
     CDS             complement(4051..5403)
                     /locus_tag="ACGE4U_002027"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFF8394155.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5676..6122)
                     /locus_tag="ACGE4U_002028"
     CDS             complement(5676..6122)
                     /locus_tag="ACGE4U_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8394156.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6358..7284)
                     /gene="cysK"
                     /locus_tag="ACGE4U_002029"
     CDS             complement(6358..7284)
                     /gene="cysK"
                     /locus_tag="ACGE4U_002029"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFF8394157.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7424..8311)
                     /locus_tag="ACGE4U_002030"
     CDS             complement(7424..8311)
                     /locus_tag="ACGE4U_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8394158.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2249 a         1725 c         1399 g         2960 t
ORIGIN      
        1 catgcaaaaa agataaacga aacagctttt ctccataata acaagataat tcatacattt
       61 atttgcaatt aaagaaacca cgtctacagg cgtggtttct tttttagcgc cttttctttt
      121 agacgcatcc gatttttccc atcttgacat attgatagca aaggacatac ctcgcacttg
      181 ggatttcttg ccgtgcaatg atatctacca aaaaaaatca gtgtatgatg tgtctttacc
      241 cataattctt gtggtacttt ccgcatcagt gtttcttcta cttccatgac cgtagcatct
      301 agtttgcaga tcctcaggcg ttttgagaca cgttccacgt gggtatctac tgcgattgct
      361 ggtatcccga atgcatcccc caatacgaca tttgctgtct ttctgcctac accaggaaga
      421 gacatcaatt cttctcttga cgttggcact tgcccatcga aacgttcaat cagctgctgt
      481 gcacacgctt tgatgttttt tgctttattt cgatacaaac caatcgtttt tattttaaga
      541 atgatttcat cgattgaagc ttctgctaac gcatcgggtg tcggaaaaga ggcaaacaaa
      601 tctggcgttg ctttattcac tgatacatct gtcgcctggg cacttaagat cactgcaatc
      661 aataactcaa atggattatt gtgcttgagt tctccatgtg cttcgggaaa cattccatac
      721 atcgtttcta gagcttccat tgttttttgt ttgcttaaca tattccgcta ctcctccaac
      781 caatttttca gtgagacttt cggcaattct tcttgttgag ctgactgttc tacttctctt
      841 tgaagaagct gctgttttct tcgtttttgg tcttcttcca cttgttgttt tgtacgtaga
      901 ttttttcttt cccacgatag caaaatgcgg tcaacgtaat taaaactata agcctgattt
      961 aatacggctt cccgcagtgc tagcttcaaa atttccggct gataatggtc ttcttctagc
     1021 cattgtccga tccgctggaa ctcgatcgaa gaaagcgggc gtccaaattc ttgttcaaat
     1081 aattgataca ctgactggat ttctttttct tgtgtttttt cttcgtattt tttttcttgt
     1141 gtttttaaat actcacctaa tagatcatag atggggtaaa gattatatcg atctgctttt
     1201 ttaccgttga caatcgtcgt ttctatcttg ataaatccat ttgttacgag acgatccagt
     1261 atttgaaaaa tctgatcttg tttgattccc atatccatag caatcatctg caaatcaggg
     1321 aaagtatctc cttcaagctg tgccatatgc aactgtagaa caaataaaaa ttcttcagat
     1381 tgtagaccga tcctatgata atttttaagt aaaagattag atatgactgt ttgtccagcg
     1441 cttaagtaat tttctaaatt caacatgatt tctcctccgc tcaatcacag tatacgtaaa
     1501 aaaaacatgg gaagcaagaa caggtttctt ttctatttac ttcactgatc ggtcagattt
     1561 acaatgtcgt ctacttgatt tttgtttgtt catgtttttg atgcgtatct tcacttttcg
     1621 gaataaataa attaagtaga tttcgctgtt ccttagaatg ttgacaagga aacaaaatta
     1681 ctatacacta tttttgtttc caagttaaca ttattgcata aaggagtttt tacatgtaca
     1741 gtttcacaaa taaacattct aaagtttttg gatacgtctc tgtcttattc actggattag
     1801 gatttactat cgtcagtcct gtacttccgt ttttaacgct gccttattct cactccatac
     1861 atcagcaagc tttctatatc actttgctca tgtctgttta tgctctagct gccttcttat
     1921 ctgcccccat tctcggtagc ttaagtgatc attttgggcg tcatccgatc cttattatca
     1981 gtttattagg atcaagtatc ggatatttgc tttttggttt aggaaattcg atatggatgt
     2041 tgttccttgg tcgtatcatt gaaggattga cggcaggaga aatctccacc ttatatgctt
     2101 actttgccga catcacggaa cctaatgaaa gaaccaaagt ttttggatgg atgggtgctt
     2161 tagtcggtat aggaacaacg ctgggtccta ttataggggg tctattagct gagttgggga
     2221 attctgttcc tatatttatt ggagcactct ttactttctt aaatgccgtt tatggctaca
     2281 catttatgct agaaagcctt ccaataaaaa aacgttcagt agatttaagt ttctcccatg
     2341 taagaccttt tcaccaatta caacaattat tcaagatttc atccgttatt cctttattga
     2401 cagcaggttt tgctgtttgg ttggcagctg gctctcttca atctatcttt tctcaatttt
     2461 cgattgacgc ctttcagtgg aaggctgggt tggttggttt aagcttctct ttgattggaa
     2521 tcttggatac tgtctcgcaa ttgttcatta tgcctcgatt gctcaaaaaa ttttcagaac
     2581 agcagatcac aagaataggg atgttcagtg aaatcctcgc ctatctattc atcacattat
     2641 ctggaattct tctactgccg attttgtttt tatttgggat tatctgttat ggtttcggtg
     2701 attctatttt tactcctgta ttcaatggcc aactatccaa ttctgtctca gataaccagc
     2761 aaggtcttgt aatgggcggt acccaaagta tacaatctct atcgcgcgtc atcggcccgc
     2821 taatcgcagg acagctttat gctgcagcac catttcttcc gacggtattc ggatttttgc
     2881 ttatgatttt tgcctattat cactacaata aaaaatagta gcttcatttg ctacttcgta
     2941 taaattctct gtatcgaccg ataaaacgaa atactgtgtc aaattcttct tttgacatat
     3001 tttcaaatac agtagcatct cgctgtaccc atttttgatg gagttgctcg tgtctattcc
     3061 ttattttttg tccgattggc gtcaattgaa aatagatttc ttttttattc tcttcgcttt
     3121 gataagtgtt tatgatccct ctagctatca gtttttttgt tagcttactg atggctcctc
     3181 tagtcatgta actagcattc gctaattttg tcacgttagg atcacggatt ttcccgatat
     3241 gttcaatcac ttctagttca tttagagtaa atcctgcaaa ttctttttcc atttctggtt
     3301 tattacgatg aataatctca ttagctaaat ccagatattc ttctattagc agttgtcttt
     3361 tatccatttc tacaccttct ttttctttca ttatattgtt tttttgtttc ctattcaaca
     3421 aagataaaaa tattttgtag gaaaacccaa tctgaaacca agaaaaaaag gggttgacaa
     3481 agcttgtcat aacccctttt tctgattaaa agatagtaaa gagacagtca tcggtaattg
     3541 tgatgatttt ttgtcatcac ttaaggatga gcggcctttt caaaaactgt ctttaaagat
     3601 catcatcagt ttctagcaca tatggctgac cttcatatgt gtactctgta ttcaatgtgc
     3661 cgtcattttc atctgagtag atgcgtctac tgaaaggaaa acgatgagaa acattatctt
     3721 tggcgtcatt tgctggcatc gtgtaatatt cctcttgcgt ttcattcaac tgagagagta
     3781 cttgatctgc caactcatca atattgatcg ttaatttctt cgaagcactt ttataatctt
     3841 ctaaaaatgc atcttgttgg acaaaatatt ctttattcat cttctttctc acctttcttt
     3901 tcaaaatcaa ataaagctgc ttgttctttt atgtaacagc tttatctttg ctgcttattg
     3961 tgacaaaaaa ttcgaagaaa gacaactaat tgtacttttc acaaaaaaag acagagggtc
     4021 gtgacaaaat tgtgtcacaa ccctttttct ttattgtgca gtttcgattt cgcctagcgc
     4081 tgcttgtgca agtaggttca agtaattcca tgggcggtca aaatgtggtt ggaagaagaa
     4141 atctgaaagt gcgagatctt ctacagtcat tttattttga acagctaaag acatggtatt
     4201 tgctgattgt gtaatgtcgt atttggacat gaactgagca cctacgatac gattcgttcc
     4261 tttttcatag actagctcca tcattacttt ttcagtagtt ggcatgaact ctggacgata
     4321 attatcttca aatacagtcg cttggacttc tagatcgttc aaagttgcgc tttctttggt
     4381 cacacctgtc gaaccgatcg tccagccaaa taggtaaagt cctgaagttc cttgagtacc
     4441 acgatacgcc attttatgat ctgtcaagtt atttcccaca agcaaacctt gacgaacggc
     4501 atttgttgcc aaaggaatat agttttgcgt gccacttgga ttgtagttga ctactgcaga
     4561 gtctccagct gcaaaaatgt ctggattact tgtctgcatg tattcattca ctttgattgc
     4621 cccgtttggc agtgtatcta cttttccttc gatcagtttt gtatttggac ggaagcccac
     4681 acataaaatg accatatctg tatcaaatgt atctgttggt gtagctactt gtttcacttt
     4741 tccttcttca tctgtgataa attcagtaac attttcgcct agcgcaagag taacaccgcg
     4801 gtctgtcaat tctttttcta agatatctgt gaatggttta tctagatatt tattcaagat
     4861 gcgatctaat ccatcaatca atgtcacttg ttttccagat tcgacaaaag cttctactaa
     4921 ttcaataccg atatatccac caccaacaat tactacacgt tttgcttctt tcgcttgtgc
     4981 aataatttca tttgcttgat tgtagttttt gcataggagt acatttttcg catcgattcc
     5041 actaatcggt gggataatag gccatgaacc agttgtcatc actaatttat cataagaaac
     5101 cgtttctgtt tctcctgttt tcagatcttt tgccgttact gttttatttt ctgtatcgac
     5161 gtctgttaca tcgtgttcca ttttaactgt tgctcctaat gaagataatt cttcaggatt
     5221 tgagtagaac aagcctgctg ggtcctttac tactccgccg acataaagtg cgataccgca
     5281 agataagaaa gaaatattgt catttctttc atatactgtc acttctgctt ctggatgatt
     5341 ttttaaaata ctttttacag ctgcagtacc tgcatgagta cagccaatta ctactacttt
     5401 catttatggt ccctccgatt aacattttgt ccttcgacaa aactactata acagaaataa
     5461 aacggataca acagtttaat gcttgtgaat tcacaaaggt ttgtttgtag tttcattcct
     5521 tttcaagcat aagaacccaa tatctacaag attcttattt taataatatt cacttttata
     5581 tctaagattg tgaataaaag acaaaaaaat aaaaaagcta gcgttttttt tacacagcta
     5641 gcttttttta ggtataacac ttgtagaaac tgtttttaca tttgcgcctt ttttgctgac
     5701 tctttacaat ccggacagag tccatacaat tccaaccgat gttcattaat ctcaaaatca
     5761 gtcagtctgc ttgcagccat ttctatatct tcaagccctg ggtaatgaaa atcaacaatc
     5821 ttaccgcagt tttcacaaat tacatgataa tgacgctttg aactgaaatc aaatcggcta
     5881 gaagcatcgc cgtacttcat ttcttgaaca aatccaatcg aagtaaataa gcgaaggtta
     5941 ttgtaaacag tcgcaacact catatttgga aaccgatgtt caagtgcttt ataaatctca
     6001 tcagccgttg gatgggaatg atgttcaatc aaatattcta aaaccgcgta ccgttgaggt
     6061 gtgattcgaa tgttctcttt ttttaacttt tcgataacct cttcgactaa cttattttcc
     6121 atgtaaacac cccacgttgt tattctcgtc ttatcatact atttttacat tttaaaatcc
     6181 acttaacatg aaaagaaaat tcaattttat tgtattttaa tgaaaaaaga cgtaaaacag
     6241 tcgcgttcac tattctacgt ctctattcaa ataatttttc tcatcataaa tttttgtgtg
     6301 ttcttttcat ctttaaaagt tttttacata aacctatctg gagaaaaacc aagtgcctta
     6361 tatctcttgg taaagggctg tagataaata acgttcacca ttgtcgggaa ccacagctaa
     6421 taccttcttg ccttttccta attcttttgc gacttttagc gcggcagcaa ttgcagcgcc
     6481 agatgagatg cctactaaaa tcccttcttt tcgcccaact tctctagctg tttccatcgc
     6541 ttcgtctcca ctaatagaaa ggactttatc atacacagaa gtatccagtg ttttaggaac
     6601 aaaacctgta ccgattcctt ggattttgtg aggtcccgct tctttccctt ctaaaatagc
     6661 agattctgct ggttctaccc cgattaattc gattttcgga tacacacgtt ttaattctcg
     6721 gccagcgcca gtgatcgttc ctcctgtacc gatacctgca acaaacgcgt ccaagccatt
     6781 tactccaaat gcttgatgta tctcaggacc agtcgtcttc tcatgcacaa gaggatttgc
     6841 ttcattttca aattgtaatg gtaaaaagta gccgttttct tttgccaaac gttctgcttc
     6901 tctgattgat ccagaaatac catccgcacc aggagtcaaa atcaactctg cgccgtaccc
     6961 tttcatcaat aaacgtcttt cgatactcat cgtctcaggc atcacgatga ttactttata
     7021 tccttttgca actcctacca tcgacaatcc gattcctgta tttccagaag taggttcaat
     7081 gatagtatca cctggtttca gtagaccatc gtgttctgct ttttctatca tacttaaagc
     7141 aatacgatct tttacacttc ctcctggatt aaaaaactca agctttacaa aaacatctgc
     7201 tgaatcttct ggtacgattt tatttaattt gacgattggc gttttgccaa tcaattcggt
     7261 gactgagtta tagatagctg tcatcttcaa ttcctccttg tctttgctct tcttacttat
     7321 cataatacaa tctgttcatt tatggcgagt gattcgtctt tgactagcaa aaaaaagaca
     7381 aagatatacc atatatggta atttctttgc ctttttatct aaatcaaacg tttagttctt
     7441 gaccaactaa taaagaataa ctggacatag acatgccatt tttcgccatt agatcatcaa
     7501 cagacatgcc aaatttttgt gcaatatccc atagcgtatc acctgacacg actgtatatg
     7561 tctgaccatt atttgtattt acatctgttg ttgaagatga tgacgttgaa gtcgtcgtag
     7621 tagtcgttgt tgttgtactt gtggttgttg tagccgaacc tgttgatggc gtatcaaaac
     7681 gggttaaatt ataagtatta atcaatgagt tcaatttggc cccataattt ggatcggtag
     7741 cataacgacc tgtcaagtac gctgttgcat cataaaaact tgttgtattg cttttccaca
     7801 cgccagcata atgataatcg cccgtggaaa aactggttga acgaagaaca ttcgcatggt
     7861 cttggaagga ttcccaataa gaagaatatt ttcggaatgg ctcattcatc gttacccatt
     7921 gtccgtttac atattctttg gttgccatat aaacgacctg tccgttatag cttcctttca
     7981 caccgaatag attataattt ggcgcactag ctaatccgga agtgccatat gcactttcca
     8041 acaacgcttg agcgatcatg actgaagcat acaagtcatt acttgcagca acttctgacg
     8101 cagaccagcc gatttgatcg ataaacgctt gaggatcagc gactgtttga gatccttgtt
     8161 catctgcatg aacatgttgc ggtaccgctg taaccatcat cggcccaact aaaagactcg
     8221 ttccaattaa aggagcaatc gcttttttat tgatcaccat ttttctttta ggtctatggt
     8281 gccgggcact gcgggtcttc agctcttgca ttcaaattcc tcctcaaaat aaa
//
LOCUS       DAXWLF010000119         8113 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXWLF010000119 DAXWLF010000000
VERSION     DAXWLF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.119"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..552)
                     /locus_tag="ACGE4U_002031"
     CDS             complement(<1..552)
                     /locus_tag="ACGE4U_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFF8394159.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
     gene            complement(568..1047)
                     /locus_tag="ACGE4U_002032"
     CDS             complement(568..1047)
                     /locus_tag="ACGE4U_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFF8394160.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1053..2077)
                     /locus_tag="ACGE4U_002033"
                     /pseudo
     CDS             complement(1053..2077)
                     /locus_tag="ACGE4U_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2094..3200)
                     /locus_tag="ACGE4U_002034"
     CDS             complement(2094..3200)
                     /locus_tag="ACGE4U_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8394161.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3429..6026)
                     /gene="adhE"
                     /locus_tag="ACGE4U_002035"
                     /gene_synonym="adhC"
     CDS             complement(3429..6026)
                     /gene="adhE"
                     /locus_tag="ACGE4U_002035"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFF8394162.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6331..6711)
                     /gene="yajC"
                     /locus_tag="ACGE4U_002036"
     CDS             complement(6331..6711)
                     /gene="yajC"
                     /locus_tag="ACGE4U_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFF8394163.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6808..7953)
                     /gene="tgt"
                     /locus_tag="ACGE4U_002037"
     CDS             complement(6808..7953)
                     /gene="tgt"
                     /locus_tag="ACGE4U_002037"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFF8394164.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
BASE COUNT         2241 a         1796 c         1401 g         2675 t
ORIGIN      
        1 gcctacgaga taacctccca taaacaaagc agagatcgaa gcaccaccag caacaagtga
       61 atacatgata aaagcagtac ttggaggaat gaccatacct gtcggagctg acgcgatatt
      121 gaccgctgtc gcaaacttcc ggtcatatcc ttcttccact tgctgaggga tcaacactcc
      181 accaatcgcg gtagaagcgg caatggccga gctagagatg gaaccaaata aagcattccc
      241 taacacattc gtatgagcca aagcccccgg cacgcgcccg acgaacaaca tcgcaaaatc
      301 cactagtttc ttagctatcc ctccattatt catgataatg cccgagagaa cgaaaaacgg
      361 aatagcgatc agcgaaaaac tattgatact tcccaccatt ttttgagctg aactaaaagc
      421 agaaacatca aacggtataa ctaacaataa agtcatcatc gatgagaatg caatacttat
      481 agcaatcggc atccctaata caagcaacac aacaaataca aaaaataaca ctaatccggc
      541 ttctattgcc attttattcc tccattttta tattttttct tgaattgctg gcttgttgct
      601 taatgtatcg attagattga tcaggctata gataatgatg agtacccccg atacaggtac
      661 tgacagataa acatatccca taggaatact taaagaaggc gagatctgcc ccattgtcaa
      721 tgtgacagca ttccatccgc catacaccat gatcatcgca gcaaatacca gaaataaact
      781 ttgaacaaca acttctaaaa ttttactttt tttgccagtt aatttttcac ttaaaaccgt
      841 tacggctaaa cgtgcttttt gtcccacaac atacgcggaa gacaacatgg caaaccagac
      901 aagaccgaat cgaacaagct cttccgtgac cgtatttgga tttcctaaaa ccgttctgga
      961 aaatacttgc cataaaacaa cagcgaccat cccaacaagc aaaacagctc ccaggatctc
     1021 taataccttg tttaaaatca ttttcattac agctacgctc cttgctcttg gatcttttga
     1081 taaaggtctt tgtaggtagg atcgtttttg aatatatcat gaagtggggc aactgcctct
     1141 cgaaacggtg tattatctac atcattgaat tggactccat gttcttcttg tgctaaagct
     1201 ttttcttctt caattgcttt tgcaaaagct tcaatttcaa aaaccgttga ttctttcgct
     1261 gcttcttcaa tggctttttc ctgttcatca gttagctgtt ctttgatggc atcattcatg
     1321 atgatgatat ctggcacacg tgtatgctca tcataggaat agtatttagc taccccgcca
     1381 tgtccagctg tatacaagac aaattcattg ttttctgatc cgttgatcaa attggattga
     1441 agagacgtgt acacttcatc acttcccata ggtacgggcg aaccgcctaa aagctctacc
     1501 attttgatag cggtttcgct ttgcatcaca cggatcttct ttcccttcaa atcttccggc
     1561 gtatggatcg gaccgtctac catataaaag ctgcgctgac cagaatcata ataggtcaat
     1621 ccggtaaatc ctaaattttt ggtcgattgg tagattggct ccattatttc ttcattatcc
     1681 atcaccttaa agaaatgttc ttcatcattg aatagataag gaacggaaaa aatcgaataa
     1741 tctttagaga aactttccaa cgctgaggca cttacttttg tgaaatctac tgctcctgac
     1801 tgtgtcaatt cgatcagttc acgttctcca cccagttgtc catctgggaa atactggatc
     1861 tgtacggaac catctgtttt ttcttttact aaacgcccaa attcattcat tgcatcaatt
     1921 actggctgtt gcttgcataa gcaaagcgca aggtaactat ctcatttacg tctgcatctg
     1981 cgacagttgc cacactagaa ttatttccgc agccgcttag acctagcaga cctaggctaa
     2041 gcgaacttgc tgcaaacagc agtgatgttt ttttcatatg ggtttccttt cttttaacaa
     2101 taagagacga gcaattctgt caaacaaagc accgttaacg actgtccata aggcattgct
     2161 gtgattccga tattccgata aaaatctaaa ctgtccccca ttcctgtccc gattgaaacg
     2221 ttttgaactt ctcctttttc atcgatttgt tcaagcaatc cttggatagc acgataagcg
     2281 acttctttat attcttgtgg taaataacgt ttatggaccg ctttcaaaat accgtaagca
     2341 aatcctgcag ttgcagaaga ttctacgtaa gaagaaggat catccaacaa cgtatgccat
     2401 aaaccgcttt cagactgata agtttttaat gcacgcactt gtgcttcaag tgtttcgatc
     2461 aataatttgc gcaatgcatc ttctttcgat aattctaaga tttcaatgat ctctggaata
     2521 gcaatcgtca accagcagtt tccacgagcc caaaatgctt cggcatagtt atgatttcct
     2581 tcaaacgtcc agccgtgata ccacaatcct gttcgtttat ccatcaagta tttgatatgg
     2641 atcataaact ggtaacgagc ttcttctaaa tattctgggc gattcaacag tttcccgatt
     2701 tttgccaaag gaagcactgt catcatcaat gtatcgtccc acaactgatt cttattttcc
     2761 ggtccatatg tggcatgctg taacccgtct tcatttgtcc gaggcatgtc attcatgacc
     2821 cattctgccc attgttctaa ataaggcaaa tagcgtgaat ctttcgtgtc ttcatacaag
     2881 taagccatag tcaatagcgg tgccattgta ttgacgtttt tgggcggagc gccttcttcc
     2941 atacgatcct caaaccattc attgaccact tgataagcgc gaggattttt tgtcatctgg
     3001 taatttttat aaataccata tagaccgaca ccttgtggcc aattccacac tgtccagctt
     3061 ttgtcatcta ctttcagtcc atcaaagtcc aacaaaaatt caccattatc gtctttgatc
     3121 gttgtcaggt tttcaacgag caggtcaatt ttttctttca ctaattcttt aggtacaaaa
     3181 atctcttctt taataatcat ctttacgcca cctttttaaa cgctttcatt ttatatcctt
     3241 aatatagcag taataccgtc cactataaat atcgcttttt gaacaaaaac tgtctaaaat
     3301 tgacctaaat gttaaataac aaaagtaact ttcagttact tggaaattaa aaaaagaact
     3361 tggagaaaca acgttttcca agttcttttt catctatttt cttatctttt ttctacgctt
     3421 tcttttgctt atccgttgta agcatcaatg atgatctgtt tcaattcaga gatcaatggt
     3481 tctttcggat tagctgtcgt acattggtct tcgtaagcta attcagccat acggtctacg
     3541 gttgtatcta gtgtttcttg tgtgactcct tgagctttta gactcatgtt gatgccgaca
     3601 gattttccta attcataaac agccgttgct aaagcttcga ctaattcctc tgttgtttcg
     3661 cctttcagac ctaagaattt tgcgatatct gcgtaatctg tatctgcacg gaaatagtca
     3721 tatttaggga acatcgcatg tttttgtgga tcttttgcat tatagcggat aatatgcggt
     3781 aaaagaatcg catttgtccg tccatgaggg atcccgtatt ccccgccgat tttatgtgcg
     3841 actgagtgac aaatacctag gaatgcgtta gcaaacgcca taccagccat tgtagaagcg
     3901 ttgtgcattt tttcacgtga ttccatatca ggtgttttta ctgatttttc taaatagtca
     3961 aagactaact tgattgcttg taggctcaat ccacgtgtat aatctgaagc catgactgaa
     4021 acataggatt cgattgcatg tgttaacaca tccatcccgg tatctgctgt gacagatgct
     4081 ggaactgaca tgacgaattg aggatcgacg attgcgacat ctggcgtcaa tgcataatct
     4141 gctaacggat atttcacatg tgtttcactg tcagtgatga ccgcaaatgg cgttacttca
     4201 gaaccagtcc ctgatgttgt cgggatacat acgaattgtg ttttttctgg tttgtcgatc
     4261 ttatatgtcc gtttacggat atccaagaat ttttgttttg caccaaagaa agatgtgtct
     4321 gggtgttcat agaacatcca cattccctta gctgcgtcca ttgcagaacc gcctccaaga
     4381 gcgattactg tatctggctt gaagtctgcc atcagtttcg ttcctgcgta tacggtattt
     4441 gtcgatgggt tcggttcaac atctgagaag acttcgatct tcacatcatt tttacgtcgt
     4501 gccaatactt cacgaactgt atcgcagtaa ccgaattgga ccataccagg gtcacaaacg
     4561 atcatgacac gttcaacgtt ttccattttc tctaagtaca atagagagtt cttttcaaag
     4621 aagatttttg gcggtaattt gaaccattgc atgttatttc tccgtttcgc tacagttttg
     4681 acattgatta agtttacagc agagacgtta cgagaaacag agttcttacc ataagaaccg
     4741 catcccaaag tcaatgacgg aatcatttcg ttatagatat caccgatgcc gccttctgca
     4801 gatggtgtat tcactaaaat acgacaagct ttcattcgaa gaccgaattt gacatgcaac
     4861 tcttcgtttt ctgaatggat aactgctgta tgtcctaatc ctccaagatt caacatacct
     4921 tcacacaaat caaacgcatg gtccgtgttg ttagctttga tcatggcaag aactggagaa
     4981 agtttttctc ttgatagcgg ataatctggg cctacgcctt ccaattcagc aatcagcatt
     5041 ttcgttcctt ttggtacact gattcctgcc aattttgcga tttccatcgc agaatgtcca
     5101 acgattgatg gattgaccgc atattttcct tcgttcatca ctgcatcttc taatttttgc
     5161 aattcatctg gttttacgat ataaacttgg tgtgcttgga attctgcttt tactgctgca
     5221 tagatttctt tatccacgat cacagcttgt tcagaagcac aaatcattcc attatcaaat
     5281 gttttcgaaa cgatcaaatc gttcactgca cgtttaattt ttgctgtttt ttcaatataa
     5341 gctggtacgt tacctggacc tacacctaaa gctggttttc ctgtagagta agcagacttg
     5401 accattccag ctccacctgt cgctaagacg atcgcgatac ctggatggtt cattagcata
     5461 gaagtcgctt cgatcgaagg atgttcgatc cattggatac aattttcagg agctccggca
     5521 gcgatagcag catcacgtac cactcgtgct gcttctgcag aagatttttg ggcacttgga
     5581 tggaaagcaa aaacgattgg atttcttgtt ttcaacgcga tcattgattt gaaaatcgtt
     5641 gtggaagtcg gattcgttgt tggagttact ccgcaaacca caccgactgg ttcagcaatt
     5701 tcaatcaacc ctttttggac atcttcattg atcacaccaa ctgttttatc atgtttgata
     5761 ttatgccaaa tatattcgga cgcatacata tttttgattg ctttatcttc gtaaacaccg
     5821 cggcctgttt cctctacagc catcttcgct aatggcatat gttgatccaa agctgccatt
     5881 gccatttcat gcacgatatg gtctactttt tcttgatcaa agctttccat ttctttcaat
     5941 gctgcatttg ctttttctgc caaatcatca atcattgctt gtacgtcaat tgtcttcgtt
     6001 ttttcttttt ctattacttt agccatcatt atcctcctaa tgggtcatgg ttatttgtta
     6061 aataattcac atgtatatca tacaatattt atttttctat gtaaacactt ttttgtgatt
     6121 tttttcacag ttatttttaa atataaaatt gctttattta caggtgtttg tatagaaaaa
     6181 tatataaagt tatttattat tatttatatg acacacccga ttattcaata ttgagcaata
     6241 tatcccatgc tcttatctct ttcttttttg aataaaaaat gaggtaggac atctttctgc
     6301 cctacctcgt ctttaaataa gctttgatac ttatttttct tccttctctt ctgtctcttc
     6361 cagcgtttct gttgtttcag tttttacttc aactgtctca gctggttctt ctgttgtttc
     6421 aacagtagtc gttgcatcat ttgttacgac agtccctggt ttgaccgttt tgattgctgc
     6481 acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca actgtccgtt tgtcattgtc
     6541 gatttctgaa acaactccat gtaatccgcc gatcgttacg actgaatcac ctggtttcat
     6601 gctatctaac aagttttgac gttcttgctg ttgtttcttt tgcgaacgtg acataaaaaa
     6661 ccacattgct aatagcgcga taaacatcac aagcattggt aatgttccca tttttttcac
     6721 ctcactaatt ctgtatctac aaaaaacact ttagcagaaa aatgctgata acactagttt
     6781 tttacttaat ttttaaaaga ttttccttta gaagcttttt gcgttttctt tattgaaccc
     6841 gtattcttca aaaaaggctt ctctaaattc aagcaaatta tcatccatga ttgcttgacg
     6901 tacttgtttc attagattca acaagaagta taaattgtgg taagaagtca aacgaatccc
     6961 aaatgtttca tcacatttga tcaaatggcg gatataggca cgtgtatagt ttttacatgt
     7021 ataacaatca catttctcat caattggacg gaaatcatgg gcatatttcg catttttcac
     7081 tactagacgg ccttgagaag tcatacatgt gccatttcga gcgattcgag taggcaagac
     7141 acagtcaaac atgtctactc cacggataac accatcgatc aatgaatcag cagcaccgac
     7201 acccatcaag taacgtggtt tgttttcggg gatcaacggt gtagtgaatt ctaatacacg
     7261 attcatttct gatttaggtt ccccgacaga taacccgccg attgagtaac cagggaaatc
     7321 catcccgact agatctttcg cgctttgacg acgaagatct tcaaaccctg ctccttggat
     7381 gattccaaat aatccttgac ggtctggatt agcatgagct ttcaatcctc gttctgccca
     7441 tcgagacgta cgttctactg attttttcac gtagtcgtaa ctttcatcga atggtggaca
     7501 ttcgtcaaaa ctcatcatga tatcagagcc caatttattt tggatgttga ttgctttttc
     7561 tggtgataaa aacatacgcg aaccgttcaa gtgatttttg aaatgcacgc cttcttcttc
     7621 gatattccgc atatcactta atgaaaaaac ttgaaagccg cctgaatctg ttaaaatcgg
     7681 ttgatcccag ttcataaatt tatgcagtcc gccagcttct tctactaagt cttcaccagg
     7741 gcgtagccat aaatgatatg tattacttaa aataacgcca gcacccattt ctttcaactc
     7801 ttctggggac attgttttta ctgtagctaa tgttcctact ggcatgaaca ttggtgtggg
     7861 gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg ccagtatgtt tttctttctt
     7921 gattaaacga tagcgaatgg caggttgcgt cattcatttc cctactttct atataaaata
     7981 tcttgacaac tcccaccatc ataatcgttt caagtgcttt ttgcaagttc cctcggcttc
     8041 cttctacttt atttaatatt ctttttttta tccttttttt atgaataaca aaaaaaagaa
     8101 aaacattatg aca
//
LOCUS       DAXWLF010000120         8042 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXWLF010000120 DAXWLF010000000
VERSION     DAXWLF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.120"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(374..700)
                     /locus_tag="ACGE4U_002038"
     CDS             complement(374..700)
                     /locus_tag="ACGE4U_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394165.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(687..1475)
                     /locus_tag="ACGE4U_002039"
     CDS             complement(687..1475)
                     /locus_tag="ACGE4U_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8394166.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(1843..2133)
                     /locus_tag="ACGE4U_002040"
     CDS             complement(1843..2133)
                     /locus_tag="ACGE4U_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFF8394167.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(2269..2448)
                     /locus_tag="ACGE4U_002041"
     CDS             complement(2269..2448)
                     /locus_tag="ACGE4U_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394168.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(2445..2795)
                     /locus_tag="ACGE4U_002042"
     CDS             complement(2445..2795)
                     /locus_tag="ACGE4U_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394169.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(2788..4113)
                     /locus_tag="ACGE4U_002043"
     CDS             complement(2788..4113)
                     /locus_tag="ACGE4U_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFF8394170.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            4563..5177
                     /locus_tag="ACGE4U_002044"
     CDS             4563..5177
                     /locus_tag="ACGE4U_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8394171.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(5342..5584)
                     /locus_tag="ACGE4U_002045"
                     /pseudo
     CDS             complement(5342..5584)
                     /locus_tag="ACGE4U_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5788..7107)
                     /locus_tag="ACGE4U_002046"
     CDS             complement(5788..7107)
                     /locus_tag="ACGE4U_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFF8394172.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(7241..7849)
                     /locus_tag="ACGE4U_002047"
     CDS             complement(7241..7849)
                     /locus_tag="ACGE4U_002047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394173.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
BASE COUNT         2472 a         1460 c         1253 g         2857 t
ORIGIN      
        1 atttgtaata attaaaatca ttcatagcca atcctctttc tgtaggatag cgttttgttc
       61 aaaactatgt tacaatgaat taaacaaata tatctatcca ctatatattt ttctactgct
      121 gataagtcgc caaactttgt taaagcagta gtcttttttt atcttttttt agtttacctt
      181 aaaaaataaa ttaaagcaaa ccgctctatt tcaaggttta agctcatagt aactatatca
      241 cgatattaca ataatattat atcacgatat cgtaatatta caatatgaag ataagcgact
      301 tatcagcttt gatactactg atttttataa aaaaaatatc acgatattat gatatcgtga
      361 tattgaatgt aaattaaatt ttattttgtt tctttgctag tttatatcct tcaatcaagt
      421 attcttcagg tatggacttt aatagcgcat cttctataac gtctggttta cttttgtctg
      481 attcagcaat agtcgtccaa tatttgagtg ctgacattag aattggattt atacgaatgc
      541 tatctgctgt tcttacacta cttccttgat cttttatgac tttacttata tttttcttgg
      601 ttaattcgat ttcttctact tcttttgatt taaaattctt tttattttta tttcctagta
      661 atctacttac tgcagcttga ctgcgattat cttctgccat caaaatcacc aacttctttt
      721 aaatattctt ctgtaaatgc tttatagtca tcttcagctg ttgagggttt attaaaattg
      781 attagatctg atccagcagc ttgcgcttcg tcaacggtta ttgtcctacg aatataagtt
      841 ttgaaaattt ttgcaccaat gactctagaa atttcggttg ctgcagacgt catttcttta
      901 gaaatgttat aacgcttatt atacttagta aatagaactc ctcgtacttc aagcttgtcg
      961 ttcgtatatt cttttaccgt ttcaatagtt tcacttaact ttattagtcc ttttaatgag
     1021 aaatacgcag cttcaactgg aatgataatt tcatcagaaa cagtaaatgc gttgactgta
     1081 agcattccta aacttggtgg acaatcaatt ataatataat catagttgtc cttgattggt
     1141 tgaagtactc ttttaagcct gtgttcactt cctacttgag taaattccct ctctgcagaa
     1201 gaaagggcta tgtcggctgg caaaacatca attcctttat agtccagaat agcatctgta
     1261 atgtcagcct ccccttttaa tacttcataa attgtaggtt ggtttgttcc atctatatcg
     1321 aaacctacat tatctgtagc atttccctga ggatcaatgt ccactaaaag tacattttta
     1381 ccgtaaacaa atttcaaaca tgctgctaaa ttaacgctag tcgtagtttt agaaactcca
     1441 cctttttgat tggctactga caatattctt gccattaaaa tatcccccat tcatcttata
     1501 ttataatata attatatcat aatattgtga tattgcaata tcgtatatgc attatattgt
     1561 aatattgtgt gattattttg ttttaaagat atatatcggt tcggatataa agattattca
     1621 tatgttattc gaaattaaaa taatcacttt ttattacttt aaaatttttt attaatgata
     1681 agaaatatgt tttgcgagca gcaactagaa taatttaaac attactaaac tgtctatatt
     1741 gttactaata attcccttct tatagtgcat tttcttagaa aaaaacaata ttacgatatc
     1801 atgatattaa gatatcgtaa tattatacaa ttgtgattga tattatatat gtttttttat
     1861 ttttttatca tgtttaatct tgaaggaatc atattctttt tctaaagaca ggagcttttc
     1921 ataaacatca gacatgttta aactagaggc caggcttaaa tcataaacga atgaagcagg
     1981 taatgatttt attctacgtt gtcgggttat ccagcttgca accgttcctt gtgggtaggc
     2041 atataactca caaaattgtt ctacagtgat tcctaaattc ttgataatgt aaacattgat
     2101 cgggtgcgag taaacaaatg tattgataga catggtgcgt aagcctactt tcttttatta
     2161 ttgcgtttgc aataatagtt taacacacgc attattctag tatctactcc tatttgtaaa
     2221 aacaaaaaag gattcataga gataactcta tagaatccgt aaaatacctt atatatataa
     2281 cgggaagata taaaacctaa aattatgata acctgctgca gcttctacat agaaagagaa
     2341 gcgctcaaca ccaacctcat ttatcaaacg ttggccataa ccccaaatct cttctgatag
     2401 ctgatcaaag gttatatact ttagctcata ttgctggata atgttcattc taatcgactc
     2461 catttattat cctggtaaaa ttcgatatga cgtatttcgt cgtagtgaat tttgtctttt
     2521 ccaacgtaga tccccagctc atcactacct aagatcttgc caatgatatc ttctggatag
     2581 tatccttcag cgtttcgttc ttctttttga atagatactg atctattttt tatgactgca
     2641 gcgttcaata cttccgcaat ctcttcagtc gtcatttgtt cttttgctaa attttgactg
     2701 gttcgttgtt cattgtcggc atcaatttct tcagtatgat cagataaata aaaccctgcc
     2761 cactttaaac gtccacgatc attgtaatca atcattttta attcccccat ttccgcccgc
     2821 atgaccacca acaagtgtgc ttctagtaat gcttctagcg ccttctaaca ttgaagaagc
     2881 atgtacaatc gacgtaaaac catatttttg ccgaatcttg tcaataattt tatctaaatt
     2941 ttcttcgtca atttgctttt gtggatcact aaataaatcc agctgaagcg aatcggtgta
     3001 cacaagcttg ctataggtta ttccaacatg ccttacttct tgtccttcat aatacttatc
     3061 aaacaagaaa agacaataat tctgcaattc ctttgtatta tctgtgatag ggatcttcat
     3121 ctggtgtgaa aaccctggtc tcgtttctaa gatcgacgtg ccaatgtgta aatggacaca
     3181 acctgtttta cagttatgct gcctaatgcg tatcgctact tgttccgcca tttcttttac
     3241 caccagctcg atttcatttc ttctagtata atctcgtggt aacacttgtg aatttccata
     3301 tgatttttcc tttgtcggtt ctggtgggat tgctatatct gttcgatcga tcccattagc
     3361 atggaagtac agctgtaagc caatcacacc tagtctattc ttaatcgtat aaggattcca
     3421 gttagcaaga tctctaatgc tcataatccc catctgattg agtctttttt tcattcgaga
     3481 gccaattccc caaaagtccg tcatttcagg aatattccat actttttcag gaacgttttc
     3541 gtacgtccat tccgcaacaa atccttcatt atgctttgct tcgttgtcta aggctagttt
     3601 agccaaaagg ggattatctc cgacacctac tgtagcaatc aatcctagct cttctttaat
     3661 attctcctgg atcatttgag ccagcttctt tctccgttga cttcgtgttc cttcagtcgt
     3721 aaaaagattc agtgatcggg tcacttttaa gattgattca tcgatcgagt aaatcagtag
     3781 atcttcatca gccacatatc ttctgaaaat attatttacc tgcatatttc gcttgatata
     3841 taggttcata cgtggtggaa caacgtgtag tgttttagga aatggttgtg gtaagtcacg
     3901 tggtctactc acatttgtaa taccataccg cttttttgct tcaggagaag aagctaatat
     3961 caatcctgaa ccagtattat cagctcgact cataacaaca agttctgttg ttaatggatc
     4021 taaatttctt tctatacatt cgacacttgc gtaaaagctt ttcatatcga ttaaaaagta
     4081 atcatttact ggctcttttg aataatccat cataaataac actccattct ttcacattat
     4141 acaaacatat gttcgatatt gtaaagaaga aatagctaaa tacataaaaa aagaagcata
     4201 ggtatgctat caccctgctt cttcttttga tttgtatgat caatttattc tatcacttta
     4261 ctaattaatt tgaaaatcta ataaaaactc tcactctaaa agagtgagag actgcaaatt
     4321 agagtacgga cttttttgga gttccgttct ttaatattaa caaatgaata aaatccttac
     4381 aacatttgag aataagttat cagtagacac ttacaaaaaa ttctcatact atttcctcca
     4441 tcattagttt ttgtctatat aaggtttacc ctaaatagac actggtataa cattgaaaaa
     4501 tatggcttcc tcgtgtatga ttagggtgta ttttaaatag ttgcagaaag taggtaaaag
     4561 ttttgagagt tgcatacata cgtgtctcat cgatcgatca aaatgaacaa cgccagattg
     4621 aagaaatgaa aaaattcggt gcagagagaa ttttcatcga gaaacaatct ggcgcaacga
     4681 ttactcatcg acctgttttc caagaagcat tagattacgt ccgagatcaa gatattttta
     4741 ttgtggaagc tattgatcga cttggaagaa attatgacga gatcatcgct tcagtcaatt
     4801 acttgaagaa gaaaaatgtg aagctgatca ttactagttt accgatcatg gctgaagcaa
     4861 ttggcaatcc tctcctggat aaattcatta aggatctgat catccaaatt ttggccatga
     4921 tcgctgaaca agaacggact gaatcgaaac gtcgccaggc acaaggaatt aagatcgcaa
     4981 aagcaaacgg cgtctacaaa gggcggccca aattatacag tgcagacgca aaagatcccc
     5041 aacgtcgcct tgtttacaaa agcatcgttg aagatcttaa aaatggtgtt gcgattgcta
     5101 agattgctaa agactacaat gttacgagac aaaccgtgta tagaataaaa aaagacagca
     5161 tggtcaatga caaatagttg atcatgctgc ttttattatc ttctcatctt taatttccta
     5221 catataatta ctatctataa aattaagtct tactcagaac agataaagca taaaattcca
     5281 acaaaccatt ttttacttaa ctaaataaag agtctgttaa tataatcttc acttatttac
     5341 gttaatcccc tactgtattt gttggttcct tatctatatt ttctacaaaa tccttattat
     5401 cttgtgattt agcttcgtat aattctttgt atgtcgacac attggttata ctaattccta
     5461 tagctagttc ttgagactct acttttctct ctagctcctt tgacaaagtg atttgagatt
     5521 tatttggttc cgttggagtc aattctatag attcttcata aaaatctcta tacataaagc
     5581 tatccctgaa taattcatag ctttaattct ttggcacatt ttattgaaat atgtatcact
     5641 aatatcccta tcaactgatt ttgactaaaa agttacttca aacattcact gttcaaaaat
     5701 attgaggaat aaattttggg tgtaaggcat tttgagcata cttctccctg gataacttcc
     5761 cagtaaaaaa atcgttagat tgttggatta gatccattga ctggctcgcc tcaggcgagc
     5821 aaagaccctg tagaattcat tctgatacac atgataacta gataatttga gatagactcg
     5881 tctacccgtt tggactaatt tcccagcaac tttcagaaac ttcaatcgaa tagaatgaat
     5941 ggtcattccc ttttggactt cttcaaagcc aatcgttctt aagaagttga ctaagttgta
     6001 agctatcaag ctgagcatca ttcggacatg attctccaaa aagcgaggac tgtctgtttt
     6061 gtcaaagtaa aagccagctt tcgcttcttt aatgaagttc tccattgtgc cacgtttggc
     6121 atagatagaa aagatggtat caggagaaac attttctgat agattcgtca cgataaattc
     6181 atgtcgaaag agtagttcgc ccgcttcacg tgttgagcgt atacacacgc gacgactttg
     6241 tgaccaggat tgtgcttgat aagtggtcga gaagtactga acttctcgtt cttcccactt
     6301 ttgattatcg ccataaagta ctgatttctc agctatttga cctagtctac gattattctt
     6361 cagtcgaata acataatgac tcttttttga ttcacacgaa tcatacacac caggtgtagc
     6421 gaacccgctg tctccacgaa ccaagatgtc agtgtttggt agagagtgat tatagtgctc
     6481 taataaaggt gtgagaaact cctttacgcc ttttgatgta tattgatttc ctgaacgtag
     6541 ttcagctttt aagaagtcac cagtcaatcc gtcaaaagct accaatggat ggtaaccata
     6601 ggtttggtag tgtgtattat aattcgtttg ttcttggtga ccaaatgtat ctgaatgggt
     6661 cgaatctaaa tcaatgatta attccgtatc attacggatg aggcgtgctt tatcgataag
     6721 ttcttggttc aaagcttgaa gttcatggat attctcctca gatagtcgat ctaaaaatcg
     6781 ggaaagtgaa gattgagaag cgagttcttt cctatctaaa acagctttaa acacaggatc
     6841 ttgtctcagg agattagctg cagaatcagc tgagtaacca gcaattaatt gcataatgaa
     6901 ctgctcaagt atggataagt tgtcatgagt aaaatacgct cgttcgtctt caatgtgaat
     6961 gtgttgctta gccagttcag aaaaaccaaa gatgttcatc agctctttca ctaggacgag
     7021 acccgaatca ctcgataatt gaccacctgt atgagaaata gtgatatttg aattgaattt
     7081 tacttggttt ttgtgtaagc tagtcatgaa aagaactcct ttcttttggt tggtttagtc
     7141 actttaacca tagcagaaag gggttctttt ttcatcactt aacgggtgaa gatgaaaaag
     7201 taattgtaag ctgccgtgag gcagtttcac atggttttaa ttaaaagtat gaattattca
     7261 ggctatcatt aattttataa aaaaaattaa atgtttttag cactgccata taacattcaa
     7321 aacattcttc tatagttggt ttacttcctt gaggagaatg aaccaattta ttacgttttt
     7381 ctatgaaatt tttaatttcc tttcttattt ctttcggaaa ctcaatatta aagtaccctt
     7441 taaacatgct ccctaataat ttgtctacag ggggagattg taaattctca aatattagag
     7501 ttaaatcagg tttctcttgt tgataaaatt cctttacccc tatttcacag gctgtaatta
     7561 tcgaaataaa cctatctcta gtatttgata tatttttact tcttttaagg agtataagag
     7621 aaaaggggat actttcgtgt ccatgcatgt aattaactaa ttgttgagct tcagaagtta
     7681 tatctctaat tatatagtta acggaattag gaagtgtctt taactctaat gaaatttcta
     7741 tccaattact atcttctaat ctgtatatgc tcgaagtata atttgattcg tcagcaagca
     7801 tcgctacatc ccaaacactg atcataaaat cttttaaacg ttcaatcatt tcagcaatat
     7861 tgctaaaatc agctaatttt tctttagaat aaaaaaaaac atttatatca gaatcaataa
     7921 attctatttt tttattacta tatcgtcatc attatcttga ccatttatgt ttattgttcc
     7981 ctgagtgggg agtacttctt tcaaattcaa gttaaattta tattccatta cttctcctcc
     8041 aa
//
LOCUS       DAXWLF010000121         8009 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXWLF010000121 DAXWLF010000000
VERSION     DAXWLF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.121"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..213
                     /locus_tag="ACGE4U_002048"
     CDS             <1..213
                     /locus_tag="ACGE4U_002048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394174.1"
                     /translation="QKSLKSINYEVFNYLGVAFGLIDSEIKSTNAGNDEDLLNYLKEL
                     TSFIDEIKTTTFNKKKYFQNQYDIEE"
     gene            267..1412
                     /locus_tag="ACGE4U_002049"
     CDS             267..1412
                     /locus_tag="ACGE4U_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8394175.1"
                     /translation="MATIKQYEKKDGTKAWMFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKRETNETFQEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLETLFQYLESKRSTYRAEYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(1497..1802)
                     /locus_tag="ACGE4U_002050"
     CDS             complement(1497..1802)
                     /locus_tag="ACGE4U_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFF8394176.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(1827..2258)
                     /gene="ruvX"
                     /locus_tag="ACGE4U_002051"
     CDS             complement(1827..2258)
                     /gene="ruvX"
                     /locus_tag="ACGE4U_002051"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFF8394177.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(2255..2521)
                     /locus_tag="ACGE4U_002052"
     CDS             complement(2255..2521)
                     /locus_tag="ACGE4U_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFF8394178.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(2776..3501)
                     /locus_tag="ACGE4U_002053"
     CDS             complement(2776..3501)
                     /locus_tag="ACGE4U_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFF8394179.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(3862..3932)
                     /locus_tag="ACGE4U_002054"
     tRNA            complement(3862..3932)
                     /locus_tag="ACGE4U_002054"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3898..3900),aa:Gly,seq:ccc)
     gene            4049..4801
                     /locus_tag="ACGE4U_002055"
     CDS             4049..4801
                     /locus_tag="ACGE4U_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFF8394180.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4817..5296
                     /locus_tag="ACGE4U_002056"
     CDS             4817..5296
                     /locus_tag="ACGE4U_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFF8394181.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(5547..7127)
                     /locus_tag="ACGE4U_002057"
     CDS             complement(5547..7127)
                     /locus_tag="ACGE4U_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFF8394182.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(7120..7864)
                     /locus_tag="ACGE4U_002058"
                     /pseudo
     CDS             complement(7120..7864)
                     /locus_tag="ACGE4U_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         2570 a         1552 c         1367 g         2520 t
ORIGIN      
        1 cagaaaagtc ttaaaagtat caattatgag gtattcaatt atttaggtgt tgcatttggt
       61 ttaattgata gcgaaatcaa atctacaaac gcaggtaacg atgaagattt attaaattat
      121 ttaaaagaat taacatcttt tattgatgaa ataaaaacaa ctacattcaa caaaaaaaaa
      181 tattttcaaa atcaatatga tatagaagaa taaagattac attttcctat caaaagcacg
      241 ccacgcaact gataggagga aaaacaatgg caacaattaa acaatatgaa aagaaagacg
      301 gaaccaaagc ttggatgttt caaacatacc ttggaatcag cccgctaact ggtaaagaag
      361 tccgtactac acgtagaaat tttaaaacaa aaaaagaagc tcaaatagag cttaatagac
      421 tacttgtaga ctttgaagag aatggactta aaagggaaac gaacgaaact ttccaagaag
      481 tgtatgatct ctggtatgaa agctataaaa ctactgtaaa agaagtcact caattaaaaa
      541 ttgagatcaa gtttaataaa tggattcttc caaaatatgg gcagtttaga attaaagaaa
      601 ttactacaaa acatgttcag aagatattga atcagtgggc taaaacaaca gatcaatata
      661 aagtcctaca ttctaccgta agccgtattt tcaaatacgc aatgactcta gggctgatca
      721 ctaaaaatcc ctgtgagctc gtaatcatgc ccaacagaaa tgttgaaaaa aagaaggata
      781 ctgtcaaaat ttattcacga gatcaattag aaaccttatt tcaatatttg gaatccaaaa
      841 gaagtacgta tagggcagag tacgataaaa ctttactgcg ctttctattt tttagtggct
      901 gtcgaatcag tgaggcttta gccttagact ggtcagatat tgactttgat tcaaacaccg
      961 tcactataaa caaaacctta agccaaacaa aatatgggta caaaatttca agtccaaaaa
     1021 cgagaaatag ctatggttcc ctatctttag acaataaaac cattaattgg ctaaagaaat
     1081 ggcaaatcaa tcaaaaaaga tatatgttaa ccctcgggat tactgatcca acaatgattt
     1141 tttgtggaat ctacaaagaa atcattacta aacacgctat ctattctcgt atgatcacaa
     1201 tctgcaagaa ttctggtatt ccatttctag gtaaccatgt aaccagacac actcatgcta
     1261 gtatgctgtt agagtcggga gtctccatga aggaagttca agaacgcctt agacactcat
     1321 caatcaaatt aacaatggat acctatgctc atctaactaa agaaaccaaa gaaaaaaccg
     1381 ttgaaaagct tgcagagcac ctaaatttct agcggaacgt attcaatccc gtattcaatt
     1441 caaaacaaaa aaaagctgaa cccttatcta ccaagggttc agctatcatt tacagcttat
     1501 tcttcttctt ccgccatgaa tgtattgaat acttcttcaa tcatgtccca ttctgcgtct
     1561 gtttcgattt gttctaactc gccttctgtt ccatcttcgt tttcgatgta tgcataagct
     1621 tgtaattcta catcttcatc ttctgaaaca ccagcaggat ataaaagaac atagttacgt
     1681 ccaaattctt cttgtccgtc aactgttagt aggatttcat ataatgtttc atttccttgg
     1741 tcatccacca atgtgatgtg ttcgtgtcct tcatggtcgt gggaatggtt atgttctgtc
     1801 ataagattac ctcttttctt ttattattaa aataattgct tacctgagcc gtctaaataa
     1861 ttttgtaaga tcatcacagc tgctaattta tcgatgactt tttttcgttt ggctcttgaa
     1921 gtatttgcct gctctactag catacgttct gcctgaacag tggttaatcg ttcatcttga
     1981 taatcaactg gcaaggagaa tagttctttg atcttttctc cgtatgacat ggaagcttct
     2041 gcccttggtc cgatggaatt gttcatattt ttaggtaatc caactacaaa ccggtctact
     2101 tcatattgtg ctactaattc cttcaaccga tcaaagccaa actcttcttc tgcttcattg
     2161 atccgaatga tctctactcc ttgagctgtc cagccaaagg ggtcactgat agcaacacct
     2221 actgtcttag aaccgacatc caagcccatt actctcataa attcactcca tgatttgaga
     2281 ggtagtcctt gacaattact tccatgatct catcgcgttc gtgacggcga atcaaatttc
     2341 gagcatcacg ataacgagga atgtaggccg ggtctccaga aagcaagtag ccgacaatct
     2401 gattgattgg attgtagcct ttttcttcca aagcccgata aacaatcgct aatgtttcgc
     2461 tcacttcttt tttgcgacta tcgtcaaaac caaaacgtac ggtttcatct gtaaaaccca
     2521 tgattctaca cctctttccc gcaaggtaat tccagcttac cttctacaac cttattttac
     2581 tcgaagaaag ggaaaactac aaacaaatat tccaaacttc cgaataaatt aagaaacact
     2641 atttgtatgt ttttttcctt ttttctgtag ctttatcaaa agtactcttt tcataaagta
     2701 tacgctatat ttctttgaat gtatgttttt acacaatttt ttcataatat tcataaaaaa
     2761 ttcgaatagc tgctactaat tttttgtttt tgttgcttgt cctgtctcat aattgatcgt
     2821 gtagccatct tgaacattgt agataaagac attgaattca atttttttgt cttcaatgct
     2881 ttctgcttcc aattgcactc ccctggctac cagttcattc ccctcaaaat gaggagtgac
     2941 acggtagcgt acatgatgat ttgtttcttt gatatatgcc gcaatatcat tttcgtgagc
     3001 aagcatgtat ggagcattca acgaacgtgt ccctgtcatc aagtttttaa tgttgttatt
     3061 ttgaccagtc agctggaaac caattaaatg gctgcgatta tataaccatt gcccgtcact
     3121 taatttcttt tgtttccaac ctgtcgggtc aatatacaaa ggctctctct tttcagtagg
     3181 gaacaaatct tttccaagca tagcattggc ttgtcctact ctgttcaatt tatcaatatt
     3241 tgaatagctt tcccatgttc ctttggaaag gctaagatcc tcttttgtaa attccggctg
     3301 attcccattg atttctattt cttgatgttt tccatcatat tctggcacat ccgctacttg
     3361 agaagcttgt ggtgtggaat caaaaatagt tgtaatctgc tgaaagatat ctgaatcttt
     3421 ttgtgtggtg gaaacaccga atgcccctgc aatcacagca gcgacgatcc ccacgatcgc
     3481 cttggttgtt tttttgctca tttctttact cctttactaa cagctcccta aatcttaaca
     3541 tatgttcccc tataaataaa tactagaaag aaatagaaac agcaaaaaat aaaacctgtt
     3601 ttaagatggc ttaaaaatac aaaaaagaga cggattcctc gtctctaatc agtcaccact
     3661 ctatctgtaa ggtatttatt attattcaaa aattttcttc atactcaaga caatacactg
     3721 atttcacaac cgcttggtaa acggtgcgca aaagcttgca tctgcggtaa taagtccatc
     3781 caagttaaaa acataagaag ttgtttttgc ttggctgttc ttattacttg ttgcagagca
     3841 gctttttcac aacctatttt aagcgggtga cgggaatcga acccgcgaca tcagcttggg
     3901 aagctgggat tttaccacta aactacaccc gcaatacaaa aacagtatac gcctctttta
     3961 ttccttcctc aagttttttt attctttacg cttctggtat gttttgtagt aaaataaaac
     4021 taatcaatca attattggag aagatgctat gacgggaaaa gaattagatc tgttgctgga
     4081 cacttgttta ctcgctggga aaatcatgat ggaaagcaat gcggagatgt atcgggtaga
     4141 agatacgatg agtcgaattg ctttagcttc tggaaatttc cgtttggtca gctatgtcac
     4201 tcaaaccggt ctctttattg gtcttgatcg tacctcgacc atccgaatgg aacaaattac
     4261 caaccgttcg attaatttag aaaaagtagt aaaagtaaat gatctttcac gtaaatatgt
     4321 tgcttcagag ctaacattag aagaacttta ctgcgaactg caagcaatcg accgagaccg
     4381 ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca gtgatcagtg gaacaattat
     4441 ggtgttattt ggcggaaaga tgaccgattt acctgtgact ttagtcattg gaggattggg
     4501 atacgcagct tatctatata gtttgaaatt ttttagaatc aaatttttat cggaattttt
     4561 atcttcttta ttgattggca tagccgctat ctacagcgta cgttttggct taggaagcaa
     4621 tcaggatcta atcattatcg gatgtgtgat gcctttagtt cctggagttc aaattacgaa
     4681 cgctatccgt gatttgctag ctggacatta tgtctcagga gtttctcgcg gaacagaagc
     4741 gatgatgact gctacgatga tcggttttgc gattgctttt attttccagc ttttttatta
     4801 ggaaaggaac acaagtatgg ggacgatatt tattcagttt tcttttagtt ttttagcgac
     4861 tgcggcattt gccgttatta caaatgtacc taggcgttca ttgatttgtt gtggtctttc
     4921 tggtgctttt ggctggatga tctactggat ctgtgttcaa ataggcggat ctgctgcttt
     4981 tggttcattg cttggttctt taggagtagc attcatcagc gatcttttct ctaaacgttt
     5041 gaaaatgccc gtaacgattt tcaatattcc cgggatggtt cctctagtcc ctggtggttt
     5101 agcttatcaa gcggttcgaa atctcgttac tgaatcctat cgagaagcgg ctcattatgc
     5161 ggtccaagca attatgattg ctggagcaat cgctttaggt cttgtattat cagaagtctt
     5221 taaccataac atccgtaatt tcaaagtaaa gcgtgacgca atcatgatga aacgaaaaaa
     5281 acggcttttt aaataaacga agtgtaacaa agaaaaggtt gtgaaagaga cgttcagcct
     5341 tgagcgatga caaaatatct cacaaacatt gcgacaagta accgcaggag caataagaag
     5401 taattatgag tattaaaaaa agttttgtaa aaatagcttc tcacattttt acataaaatt
     5461 gacttaatac tgagaaactg acttttgaac accctctatc tgtaaaaaag gggctgcgac
     5521 atgatttttg tcacggcccc tcttctttat tcaaattttt tccaaaacgt tcgttgataa
     5581 taaacgatcc atagagcgga aagtattaga actaaaccga tcacaggaat attcactaac
     5641 caaaatggat aaaacaaagc caacattccg tataagacga taagaataat actgatgatc
     5701 aatgaaccca tcatagttgc gactaaaccc aaacttcctg aacctctgtt aaataattgg
     5761 ttaatattgg tccagttcag cagcagcaac cgataatctc tagcgaaata acgtagtgac
     5821 aataagtagc agccgaataa agaaccaaat agcaagctag ggataaaaaa caaaggcagc
     5881 tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta aaaaactctg caatatccat
     5941 ccgaatctga acttttcttt taaatattgg ttcatagaga ttggcaaaga acggataaac
     6001 agaaaatttt cttgatccaa agaaataaga ttgctgataa aagaagtctg attaactgtt
     6061 aagaaagcca atgcgatccc taccaggaaa acaacaccga ttaacttatt atctaaatca
     6121 gttaatttta tttcgcctcc tagggcaaaa gcaagaataa aaatgattgg agttaacaag
     6181 gaagaagaaa gaacttgcat aatcaaatta ggttctttta ctaattgagc attgtaacta
     6241 aataaaagct gatgcacatt ttggttcttt ttatgggttc tttttatttt gaactcactg
     6301 tttgaatcag tcagttgctc tgtaagcttt ggtactatca gctgttttac agcaaggaaa
     6361 aatcccagag ccgtgatagc tagtattccc caacccaata aaccttgttg agaaagtggc
     6421 tgactggcag catcatagaa tggcaataat atcgaaatcg gatgccgatc tgttaactca
     6481 ttttcaatag tccccgtttg atgattcatc cacatgattc cagcaaatgc gatcagcatc
     6541 gagctgccaa gcaagatact agaaactgtt tttctatgtt ttttaaacac tgctgttcga
     6601 gccaaaccaa aaacaatcaa actagccaga caaaaaagaa taatcaaaaa tagagaaaac
     6661 aaaaggattg agataatgat agtaagcaaa atccctagat cagctcgcca tccggttaaa
     6721 ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg tcaaagcaac taccaaaatc
     6781 tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca aaaatacttg caggtcattt
     6841 ccttcaaaaa agatattata aatgactgat atactttgag ttaaacctaa cataccaaaa
     6901 agtgccacgt aataagtaaa gaaacctggc aatttaacaa agttcatagc aaacatagca
     6961 aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat tttgagacaa aagatagata
     7021 gttaatcgct ttcccttttt tcctttctct cttgctttat tcgtgagctg tggattaaca
     7081 tataaaagat taactcgaat taattctaat aattgtcgct tatacatacg gtccatctcc
     7141 tgtcgcttgc cttccagcca ttttcaaata aatcttttct agcgtttcat caggtgagcc
     7201 attcaataat tcactaactg ccccctgata aatcaattca cctttcttca aaatagccag
     7261 tttatcgcat aactgttgtg cggtatccaa tacatgtgta gaaaaaatga ctgtttttcc
     7321 ttttgcggca tgagccttca tcatttcttt caaatcaaat gctgcttgag gatcaagtcc
     7381 ttgcaaaggt tcatccaata cccaaatatc tggatctggc agcaacgtgc cgatcaaaat
     7441 cgttttttga cgcattccat gtgaaaaact tgcgattgtt tcattttgat gactatacat
     7501 atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt tttttgtgtg tctaattcat
     7561 aggcagctga aatcaagtcc caatattctc cagccgtcaa ttgtaaaaaa atatctgggg
     7621 aatcaggcac ataaccgatt ttttgtttga tactcaatcg attttccgtc aatttcattc
     7681 catctactaa aatcgttccc gatgatggtt caataatact aactaaactt ttaatcgtag
     7741 ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat ttccccttgt cttatcgaga
     7801 gagacaagtc ttttaatgct tgttttttcc cgtaactttt acttacattg atgaattcaa
     7861 tcatttctgt actccttttg tttgttatta ctttttattg tacccttcgt tattggtgga
     7921 gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa cgactaggct gctacttggg
     7981 ttgttctctt ctacaggaaa acactcttc
//
LOCUS       DAXWLF010000122         7985 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXWLF010000122 DAXWLF010000000
VERSION     DAXWLF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.122"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..1125
                     /locus_tag="ACGE4U_002059"
     CDS             <1..1125
                     /locus_tag="ACGE4U_002059"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFF8394183.1"
                     /translation="FIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAVNRANEDIITL
                     GANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFARSFEVKEACY
                     GGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAMMITQNPRILS
                     IEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKELSGRGLEDYQ
                     AIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNLYTGSLYLGLT
                     SLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQEMLDSRTRITV
                     DEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1147..1899)
                     /locus_tag="ACGE4U_002060"
     CDS             complement(1147..1899)
                     /locus_tag="ACGE4U_002060"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8394184.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2263..4929)
                     /locus_tag="ACGE4U_002061"
     CDS             complement(2263..4929)
                     /locus_tag="ACGE4U_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8394185.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5270..6721
                     /gene="cls"
                     /locus_tag="ACGE4U_002062"
     CDS             5270..6721
                     /gene="cls"
                     /locus_tag="ACGE4U_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFF8394186.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7050..7865)
                     /gene="thiD"
                     /locus_tag="ACGE4U_002063"
     CDS             complement(7050..7865)
                     /gene="thiD"
                     /locus_tag="ACGE4U_002063"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFF8394187.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2311 a         1678 c         1432 g         2564 t
ORIGIN      
        1 tttattccta atttatattt agatatgaca gagttggcag aaagccgtgg ggatgatcct
       61 gcaaaatacc atatagggat tggtcaagac caaatggcag tcaatcgtgc aaatgaagac
      121 atcattacac taggagcaaa tgctgccagc aaaatcgtaa cagaaaaaga ccgtgagcta
      181 atcgacatgg tcatcgtcgg aacagaatcc gggattgatc attcaaaagc gagtgcggta
      241 attatccacc atttactgaa aatccaatct tttgctcgtt cttttgaagt aaaagaggct
      301 tgctacggtg gcaccgcagc tttgcacatg gcgaaagaat atgtcaaaaa tcatccagaa
      361 cgaaaagtac tagtcatagc aagtgatatt gctcgttacg gcttggcaag cggtggtgaa
      421 gtgacgcaag gtgtcggtgc tgttgcgatg atgatcactc aaaacccgcg tattttatcg
      481 attgaagacg acagcgtatt tctgacagaa gatatctatg atttctggcg tccagattat
      541 agcgaatttc ctgttgttga tggtccttta tctaattcta cgtatatcga atcattccaa
      601 aaagtttgga atcgacataa agaattgtcg ggtcgaggac tcgaagatta tcaggcgatt
      661 gctttccata ttccgtatac taagatggga aaaaaggcat tgcaaagcgt attagaccaa
      721 acagacgaag aaaatcagga acgtcttatg gctcgctatg aagaaagcat ccgttacagc
      781 cgacgaatcg gtaatcttta cactggttca ttatacctgg ggctaacttc tctactggaa
      841 aattcgaaat cactacagcc aggagatcgc atcggtctat tcagctacgg ttctggtgca
      901 gtaagtgaat tttttacagg ttatctggaa gaaaactatc aagaatatct cttcgcacaa
      961 tctcatcaag agatgctaga ttctcgaaca cggatcactg ttgacgaata tgaaacgatt
     1021 ttcagtgaga cgcttcctga acacggagaa tgtgcagaat atacttcaga tgtacctttt
     1081 tcgattacaa agatcgaaaa tgatattcgt tactacaaaa tataataaaa tcataaaaag
     1141 aacccttcac gagtcgtgaa aggttctttt tttatctgcg gaccaaagga gcagtctata
     1201 ggtcagctta gtcgtactaa ggatttctcg tttttcactg aatagtttgc gaatgtacct
     1261 ggtcacaaca ggatcttttg cgtacgcgtc atagtatagc ttcatcaatt cttcagtaat
     1321 tgattcctgt gtaaaataag taaaatcttt tttcttcact ttgaatcctt catcaattaa
     1381 gtatgtttca aattgatcca ttacaactgg cgatgtatta ggatcttctt ctatcattcc
     1441 tagttgcttt tcgataggat aaaaaacatc atcgattgat tccactactc tgattatcac
     1501 gcaccatttg cttgactttt tcatcaattt ttctaattga ataagatgta ccaatgctgg
     1561 atttgctgct gtaaaaacca tttcatatgt ctcatttgat tggataaatt cttctaacga
     1621 ctgtttgata aaggaaacat ttggcaactc atttttttct gcttcttttt ttgcattttc
     1681 tagcattcct gaagaaaaat cgatcaaatc atagcgatta atttcttttg ctaaggaaag
     1741 aaatctgcca gatcctccag caacgtctgc tgctgtttca gcaggcagga ctttctccgt
     1801 tttcagataa ttttttacat cttcagcaat agttacttgc gattcttgct ggatttcata
     1861 atattcttca gcaaattcat cccattcttt ttcattcatt tgatttcctc taattttttg
     1921 ttatatgttt tcacttttcg gaataaatca ttctcctctt ttatctattc tatctctttt
     1981 gatgaataat aaaatgattg gctttccgtc tatcataaat aaaaaaaggg actgtgacca
     2041 aagtcgtgtc aaagtccctc tttccgaata aacagtgtcc aatcagatga ccctcagtat
     2101 tagctcaaaa tgctgatttt acaacttctt ccttaaacgg tgttctcctg ccaactcttc
     2161 ggtcacaagt cacaatattc aacaaacatg agaagctgtt tgttgaaatt gcaccttgtg
     2221 actcagaatt aaacggcagg ctcacaacct cgttgattcg ttttatttat ttccttgtac
     2281 tcgtacaatg atcaatttag tgatttccat caaaacaatt gtcacaagtg cgattcccat
     2341 acatgtaaag acttctgtca atccaaatgc tgatgggata gagaagactt ctttcagtcc
     2401 tgggatcatt gttaagcagt aaagcgcgct acatacaact acagctagta agacacttct
     2461 gttagagaag aaccctgctt ggatagctgt ttgtgtgttt gaacgtgctg ggaatgtttg
     2521 caatgtccga caccaaatca atgttgaaaa tgacatggct acaccgaatt caggtgaatg
     2581 ctgcatgcca agccattgag caaagataac agcaatccca atcagcacac cacgatacgt
     2641 tacagaaatc aatgttttgt ccgtaaagat tcctgaattt ggatctcttg gcgggcggtt
     2701 catcgtagca ggttcagctt tttctgtacc taatgcaatt gctggcagtg aatcattgac
     2761 taaattgatg aacaacagtt gtaatgctgt aaatggattg gaccagtcag caactaaggc
     2821 aaagatgatc gcaatgatcg cacctaaatt tcctgcaaat agataagaaa tagctttctt
     2881 gatattatca aacacattac gtcctacttc tactgcgctg acaatagaag caaagttatc
     2941 atccgtcaat accatcgctg ctgcatcttt tgccacatct gtacccgttc ccatagcaat
     3001 tccgatgtct gcttgtttca atgctggtgc gtcatttaca ccatcacctg tcatagctga
     3061 gatatgacct ttattttgcc aagcacgaac gatacgaatt ttattctcag gtgaaacacg
     3121 tgcataaaca gtgacttttt ctaaaacttc gtttagttga tcttctgtca attcatctag
     3181 ttccgtcccg gtcaaagcca ggtttccttc ttcaaaaatc ccgatttgtt ttgcgatagc
     3241 aacagctgtt gttttgtggt caccagtaat catgattgtt ttgattcctg cacttcttgc
     3301 ttctgttacc gcctgagtca cttcagcacg tggaggatcg atcatagcaa gcaaaccaac
     3361 aaggatcaaa tcgtcttcat cttcaaatga taaatcaaaa ttatcaagtg gacggtaagc
     3421 aaaagctagt acacgtaatg cgcgtgttga aaaagcttca ttttgctctt ggaatttttt
     3481 catcaattca tccgtcatcg gaacgacttc accatttagc aatacttttt tactgcgttg
     3541 gaagacgata tctggtccgc ctttaacaaa caagtaattt ttgttatcaa ttgtgtgtcc
     3601 agtagacatt aattttcgat ctgagtcaaa aggaagttct gcttgtcttg ggtagttgtt
     3661 tcggatttcc tgataaggct ggtttaattt ttcactaaag tcgataaacg ctacttccgt
     3721 agggtcgccc aatttagtac cgtcttcact gatcgatgca tcattcgcta aaacactgat
     3781 ttcgatcaag cgacgttcgt cagctgtcca ttttgaaggg tcatttgtga aatccccagt
     3841 atcgccatta cctaaaaagt agtcaacaac tgtcattttg ttttgtgtca atgtaccagt
     3901 cttatctgta cagatgatac ttgtcgaacc aagtgtttca actgctggaa gcttccgaat
     3961 aatggcatga cgttttgcca ttttattcgt tcccaaagat aacacgattg ttacgataga
     4021 ctgtaatgct tctggaatag cagctacggc tacggctacg gcaaacatca ctgcgctgac
     4081 gatatctgca gtcatatctc ctgtaccttc tccaagataa atacgaaaca gttggatacc
     4141 taaaatcaat aaagacaatg ccaaaatagc caaacttagt tttttactga atttatccag
     4201 acctcgttgt aacggagtga cttgctcagt tgttgattcc aaaaggccag caacttgtcc
     4261 gatctccgta ttattcccag ttgaagtcac taagaattta ccgcgtccgt aagttacgat
     4321 cgttccactg aataccatat ttgtacgatc tccgattggt accgtacttg tgatatccgt
     4381 tacttctttt tctgcagaag tcgactctcc tgtcagcata ccttcatcta cttttaatga
     4441 tcccgcatct agtaaacgtc catcagcagg tacataatcg cctgcgtcta acaacacgat
     4501 atctcctacc acaagttcgt ttgccggaat cgttttttcc ttaccatcac gaatcactcg
     4561 agcatcgggc gctgaaaggt ttttcaacgc ttctaaagaa ccttctgctt tttttgtttg
     4621 gacaacactg attatagaat tcaacatcaa tactgcaaaa ataactaaga attcgatgta
     4681 gtcacccatg accatctgaa tcagtgcgac aatcagcaat acgataacca ttgcatcttt
     4741 gaaagtttct aagaacaatt tccaagttgg cttcttcggc ttttctttta gctggttcaa
     4801 gccatctctt tctaattttt ccttcgcttg cgtggtgctc aatccatcgg ttgagctctg
     4861 cgtttgctgc atcaacgagt cgatcgtttc tttatagacc gcttgttgtg cttgttcctt
     4921 gttttccaaa tttcatcccc cattttttga tcttggccct agtcctgtct gaaaaaaaag
     4981 agacctaaga caaatacgca tttgtcttag gtctcaccat ttcatacaat accagaaaac
     5041 catttggtta acttactgtt gatattatcg caacaaacgt tgccggttac tcccctaagg
     5101 agatattcaa ctgtactaat atcctacctt attaacctac aaaaagcaac tactttagcc
     5161 cgatttacct acattttcat gaaaggagtg aaaagtatat tcaccttggt aggcttcttt
     5221 cttctatcat gctatactaa tgttattgta tgtaaggagg aacatttttg tggtatctag
     5281 tattataacc gccctttatc tattaaatgc acttattgct ttggtggcta ttttgatcaa
     5341 accccgagat gtagcagcca tttgggcatg gctcttagta ttgtttgccc ttcctggggt
     5401 gggctttgta ttatatttat ttttcggacg tggattaacg gataagaaaa aattctatct
     5461 ccgacaaagt gacttgaaag aattagaaaa ctttcagtct tttagaggag ataccattga
     5521 acattacgat cctgacatgg gcgataaaga caaacagcaa tttgttgact tcttctcttc
     5581 attaaatcgt atgccgctga caagaatgaa ttctgtcact cttctcacag acggacaaga
     5641 gaaattggat tcactgcttc aagatctaaa aaaagccaaa cattcgatcc atatcgaata
     5701 ttacgcattt gtgacagata atatcggcca gcaagtctta catgttttag aagaaaaagc
     5761 cgcagaaggc gtggaagttc gaatattata tgatgcattt ggctctcatg gcacaaaagc
     5821 aaaagatttc aatcgtctaa tcaaaaatgg tggacatgtc catacatttg ttacctcaca
     5881 aagggcatta cttcgtttcc gattgaatta ccatgatcac cgaaaaatcg ttgtgatcga
     5941 tggaaagatt agttataccg gtggtttcaa tattgccaat caatatgtaa atacaacaaa
     6001 aaaattcggc tattggcgcg atacgcatat acggattttc ggtgccgctt cttctttgct
     6061 ccagcttcgc ttcttaacag actggaacgt ctcggtacct gaagaaaaaa aggtcggcta
     6121 tcatttgaat tatttcttta aaaaagcaga tcgagatgaa tctaagcttg ctgatacatc
     6181 catccagctt gtttcaagcg gaccgaataa cgaaagggaa caaatcaagc tttcatttat
     6241 caaattgatt acttctgcta aaaaacgtgt ttggatacag acaccttacc ttgttcctga
     6301 tgaaagtgtc attgctgctt taaaaatcgc aactgcctct ggtgtagatg tgaaaatcat
     6361 gattcccaac aaaccggacc atccttttat ttatcgagca acacaatatt atgctcggca
     6421 gctgatcaag gaaaatgtac aaatccttgt ctatgagaac ggcttcctcc atgcaaaaac
     6481 attgataatg gatgatgaaa tctgcatggt aggttcagca aatcaagata ttcgtagcta
     6541 ccgattgaat tttgaaacaa gtgctgtcat ttacgatcct gagtttttag aagaacttgc
     6601 tactcagttc aaagaagatg agacacattg ttcatccatg acaactgaaa cagtcaagga
     6661 aatgtctaac tggctattat tcaagcaaca aatttctcga ttattttctc caatcctata
     6721 atttttttat tcaagtagag aaaaatatac taagattaaa gggactgtga caaaaatctt
     6781 gtcacagtcc cttcttccga acaaacggtg ttctcctgcc aactcctcgg tcacaagtca
     6841 caatattcaa caaacatgag aagctgtttt ttgaacttgt tccttgtgac tcggagctgg
     6901 acggcaggct cacgacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa
     6961 gtccaaccaa gttaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg
     7021 cagggcaact ttttcacaac ttctttcttt tactctaatt caccagctgc ccaatgattg
     7081 atggggccat atttgtgccc tacatttatt tcttcacgaa ttgcacggtt tacatagtct
     7141 tttgccaatc ggatcgcttg ttcaactgtt tttcctttcg ctaactcagc tgcaatacat
     7201 gcagatagcg aatcgccagt tccgttgatc cggtcggtag gataaaatgg tgcatttaac
     7261 caaaatgatt ttcctgattc gaacaatacg tagtcattga ctgtttcttg ctgcgcattt
     7321 ttagcatgat gtcctttgat cataacgttt tttgcaccca tatcttgcag ttcttttgct
     7381 gcttgcacaa aatcttcttc agctgctaat gtccgtccag tcagcttttc tgcttcataa
     7441 aaattaggtg tcagtacttc ggccaaaggg acaagctttt ctttcaagct ttgcaaagca
     7501 gattcttcta gtaaaagatt tccatgtttc gttacgatta cgggatccac gactaatggt
     7561 ccaaaatcat atttttggta attttttaca actgtctcaa tcaactttga atctgctagc
     7621 atacctgttt tagctgcacg tatttgaaag tccttcgcaa tcagttcaaa ttctttgtcg
     7681 ataaaatctg ttggcaatgt cacacttgct ccgattccgt aagaattacc tgcaacacat
     7741 gcggtcatga cagaaacacc gtaaacattt cttgtaaaga atgtatgcat atctgcttgc
     7801 atccctgcgc ttccatcgct atctgatcct gcgatcgtca ttgcttgtac atactgatct
     7861 tccatcaaat cgattcctcc atttttataa aaagattttt cataatactg ttactgtttc
     7921 tatcatatgt ttttcaaagt atccctcagt aaatatacca ctaccttttc ttggtaatca
     7981 aattt
//
LOCUS       DAXWLF010000123         7913 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXWLF010000123 DAXWLF010000000
VERSION     DAXWLF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.123"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..257
                     /locus_tag="ACGE4U_002064"
     CDS             <1..257
                     /locus_tag="ACGE4U_002064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394188.1"
                     /translation="KAVIFMYVNYILIYLSSILRSESMEKPFVKSLTAVAVGAGAIAI
                     CLFGYHINNQRQHQQRINYAESAITNQKDTLTSLSRLFVK"
     gene            complement(307..1608)
                     /locus_tag="ACGE4U_002065"
     CDS             complement(307..1608)
                     /locus_tag="ACGE4U_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFF8394189.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            1735..2352
                     /locus_tag="ACGE4U_002066"
                     /pseudo
     CDS             1735..2352
                     /locus_tag="ACGE4U_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2398..3342
                     /locus_tag="ACGE4U_002067"
     CDS             2398..3342
                     /locus_tag="ACGE4U_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8394190.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            3358..4137
                     /locus_tag="ACGE4U_002068"
     CDS             3358..4137
                     /locus_tag="ACGE4U_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFF8394191.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            4149..4847
                     /locus_tag="ACGE4U_002069"
     CDS             4149..4847
                     /locus_tag="ACGE4U_002069"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFF8394192.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            4875..5639
                     /locus_tag="ACGE4U_002070"
     CDS             4875..5639
                     /locus_tag="ACGE4U_002070"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFF8394193.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            5652..7469
                     /locus_tag="ACGE4U_002071"
     CDS             5652..7469
                     /locus_tag="ACGE4U_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFF8394194.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            7480..>7913
                     /locus_tag="ACGE4U_002072"
     CDS             7480..>7913
                     /locus_tag="ACGE4U_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid O-acetyltransferase NeuD family
                     sugar O-acyltransferase"
                     /protein_id="HFF8394195.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNS"
BASE COUNT         2666 a         1455 c         1646 g         2146 t
ORIGIN      
        1 aaaaagctgt catttttatg tatgtgaatt atatcttaat ttatctatca tcaatactta
       61 ggagtgaatc tatggaaaag ccattcgtca agtcattgac tgctgtggcg gtaggtgcag
      121 gtgctattgc gatctgtttg tttggttacc atatcaataa tcagcggcaa catcagcaac
      181 ggatcaatta tgcagaatct gccatcacca atcaaaaaga caccttgact tctttatcaa
      241 ggctctttgt caaataggac tgatagagtg agataagaac atctaaggta gaattccttt
      301 tctactttag atgttttttg attcataggt aatcagatta tttagtaaga aaattctggt
      361 cttcattttc cgaaagcttc gaaagccata agcgactcgt ttcaatgctt tgatgtgggt
      421 atgcagattt tctattttgg catttgaata aggcaattcg agggctaaac gaatgccgga
      481 ttcatagttt ataaaagctt tttttataga atgcagttct tttggaacgg aattgggcat
      541 cgttcttaca agtttaaaga aagtgtccgc ctctttgtcg tgaaacgctt gaagaatttc
      601 ttgatagtaa aaataggcga ttcgtaattc gtcagagatc gaaagcagac gatccaccac
      661 ttcactttcg gtgagatacg cccaaccaaa attgcgccat ttggtccatt tgtcataaac
      721 aagcttgtcc tgtcgtttga tcagaagccg agacaacgcc tttaattgtc gataatattt
      781 ggcttgtgtt ggatcgtttc gatcaaagct tttcatgatt cgtacgcgaa tatcctcaaa
      841 tttacgaatg agatgtttga caatgtgaaa acgatccaca acgagttttg cctttggaaa
      901 acattccttt atcagagagg cataagaaac attcatgtcc gtcacgatga cctttacctt
      961 caatcgtgcg cgtcgttcaa attgcataaa ataagaaacg agagcctttt tcttccgtga
     1021 aggaagaata tccagtaatt ttccagtatc gccatccata caagaaaagg agtagggacc
     1081 gacttgattt ttcagtgaac gaaattcatc cattaataaa caacgcggta accaatttct
     1141 ttttactttt gtcatgggga tcagagaagc gagtgtccga gaaacggtcg accaagaaac
     1201 gccgcaatgc ttagcaattg tcgtcattgc ttgtttttca gttagctctt ctaaaatttt
     1261 atagcgcact tgtttagcga taaagcagtt tctatcgaca atgtaggtat tcgccgtaaa
     1321 ataggtgtgg cagccacgac agtggtagcg ttgtttcgct aaagcaagat aagtcagttg
     1381 gttcgcacat ttattaagtt gaatgagcga ttttttcttt ccgttcttaa cgatcatgcc
     1441 ttcatgattc atctgtttac acaaaggaca gcgacgaatt tctttactta acgtcgcaga
     1501 aacaacaaga ctcttgatat tcttaatgct cttcgattcc acatttgtga tgaaaatgtt
     1561 tttatctttg attccaagta gtagtttgat atgatgaaga tcaggcatac agaacgctcc
     1621 tttgattgtg ttgtggtgat tcaattctaa cggagcttct gtatgttttg ttttttcatg
     1681 ctcaaaaggg accgatagaa tgattttcta tcagtcctag aaattataga gcctttatca
     1741 aaagaagtag acaagctgta tagtacaaaa gaaaaaaatt ttctaaaccc tgaaataaca
     1801 gaagaaacaa tcacaagttt gtcaaataag ctatcctctg tcaaactttc agcagatgat
     1861 tacgacatca aagaatcaga attgcctaaa gaagctgttg caatccaaga tgagaagaaa
     1921 gcaatcgtga cacagttaga tagtgcagaa tcaaaactga agatccagac agctgtcaac
     1981 gatttattta ctaaaaatgt atcaaactgg cagcaagctg tagacgatgt cgtcatcaaa
     2041 gaaaaggtga caagtgcaga tgtcgctcgt gtacgtgaaa atatgagctt ttttaaagat
     2101 agtccttgga aaacggttgt tatgcaatat ctaggattcg ctgatactca gatcgcgcaa
     2161 gtcacgcaac tagatcagtt attcgatacg atgttgaagg atggtcaagt gactgccacc
     2221 gctacttacg atcaatattt gacggcgctc agtcaaatag agcagatccg gaatgaaaaa
     2281 ataagcgcag catacgcaac aaaagcacaa actgttgctc agcaaatggg ctatagcaat
     2341 ataagttatt gacagttaaa acgttgattc aagcaaggtt agcaaacgga ggaaactatg
     2401 gcaagacgaa gaagaaaatc aagagggaaa aaaatatttc tgattatttt agcagttctt
     2461 atcgtattag tattagcaat tgtaggagta ggagcaaaat tatatctgga tctctcacat
     2521 tctattgaga aaacttacga atcagtggaa cgaacccaag gagaacgaac gaaaccagtt
     2581 gatctggaca aacaagaatc tttctctgtt ctattgatgg gcattgatac tggagattta
     2641 ggtcgggtcg atcaaggaag atcagatacg atgatggttg ccacagtcag tcctgaagac
     2701 aaacagacaa cgatcgtcag cattcctcga gatacgtatg tcgacatcgt aggtcgtgac
     2761 acaaaagaca agatcaacca cgcttacgca ttcggcggag ctgccatggc gatggatacc
     2821 ctacaaaaat atgtagacat cccaatcgat cactatgtct ctatcaatat gaaagggcta
     2881 aaggaactcg ttgatgcagt tggtggtatc gaagtcaata atgacctcac tttctcacaa
     2941 gatggctatg actttccaat cggtaaaatt acattgaacg gggatcaggc attatcttat
     3001 tctcgtatgc gttatgaaga tccaaatggt gactatggta gacaagagcg tcaaagaaag
     3061 atcgtagaag gtgtagcaaa aaaagtattg agtttaaacg gtgtcagcaa ctatcaatcc
     3121 gttttgaatg ctctagagtc gaatatgaaa acagacatga gctttgatga aatgaaaaaa
     3181 atcgctttag attatcggga ggcatttggt acgatcaagc aagatcagct gcaaggagaa
     3241 ggcttcatgc aagataatat ctcctatcaa aaagtcagcg atcaagaact tgatcgtgtg
     3301 aaaaaagaat taaaagatca aatgaatcta gagaataaat aggaaagtgg agaaaaaatg
     3361 gaagagacaa tcagcttgaa agaaattttt gaaacattac gcaaacactt ggtaacaatc
     3421 atcatctcaa tgtttgctgg cttggccatc tcaggaatcg taacattttt catcttgaca
     3481 ccaaaataca gttcacaagc acaattgatc gtgacactgc cacaatcaga cacagcaaat
     3541 gtcaatgatg tcaatacgaa cctgcaaatg atcaatacgt ataaagatat gatcgtcagt
     3601 gatttagtac taaatgaagt caaagatcgt ttagaaacag aagacaatgt gaaaatgact
     3661 gctggacaaa tcaaagatgc gatctccgtc aatcagtcac aaaattcaca aatgttctcg
     3721 atccaggcaa taagcacgaa tgcagtgact gctcaacaga tcgctaatac aacttcacaa
     3781 gttttccaag aaaacgccaa agacgtgatg aatgtcgata aaatctcgat tatttcaggt
     3841 gcagttgcca atacgacacc tgtttcgcca aataataaat tgaatctagt gattggctta
     3901 gtgttaggaa tgatggtagg cgtaggtctg gcattcttat tcgaattact tgatcggaca
     3961 gtgaaagatg aaaaatatgt gacggataca ttaggattcc caatcttagg aacagttcca
     4021 gaaatgtcag caaaagaatt gaacgctggt atccgtaaaa aaggatcagc tcctgcacga
     4081 ccaaaagcta caaacgataa aacagcccca tcacgcagaa gccgctcaag agtctaggag
     4141 gaagaaaaat ggcacgaaca cagaaacaaa aagcaactca aactcatgca gtcagtttga
     4201 tcacattaac aaacccatct tcaccaatcg ccgaacaata tcgtacgatc cggacgaata
     4261 tccagtttgc ctccgcagct ggtcagcaaa tcaaaacgat tgtcgtgact tcatcaggtc
     4321 ctggagaagg aaaatccacc actgcagcaa acattgccgt tgtttttgcc aaatctggtc
     4381 aacgggtact gctagtcgat gcagacttac gtaagcccgt catctataaa acgttcaagt
     4441 tgaacaacgc aagcggatta agtactgttc ttagttcatc aggaagtgta gcagatgaga
     4501 tccaacgaac accagtcgac aatttgtcga ttctgccaag tggacctaaa ccaccaaatc
     4561 catcagaact actaagttcg ccaagaatgg atcagatttt agcagaagca agacaactat
     4621 ttgatgtagt gattttcgac atgccaccag tcgttgccgt aacagatgca cagatcatgt
     4681 cttcaaaaac ggatggcacc ttattggtcg tacgggaaaa tacgtcacga aaagaatcat
     4741 tgactaaagc aaaagaactg ttagatatgg tacaagcgcg agtccttggt gttgtataca
     4801 atggagcaga acacagcaga gatgctggct attactacgg gaattaagaa ataaaaagag
     4861 aggagacaag agaaatgatc gatctacatt gccatatcct gccaggaatc gatgacggag
     4921 cagaaaacat agatgccagt atcgccatgg ctgaaaaagc gattcaacaa gggatcacac
     4981 atattttatg cacaccccat cacaataatg gaaaatacag caatgaaaaa tcacaagtca
     5041 tttctctagt cgcttcgtta caagcagaat tagagaaaag acaattgcct ttgacgttat
     5101 tggaaggaca agaagtacgt attaccggaa cactgattga agacatccac cgagatgaaa
     5161 ttttatttac agatttagat gatacatatc ttctaatcga atttccgaca ctggaagtgc
     5221 cgttatatgc agaatggctc ttttttgagt tgttacaact aggaaagaca ccagtgatcg
     5281 tccatccaga acgcaacgcg cattttcgag aagacccaaa tcatttgctg ccattcctag
     5341 atatgggatg cttggctcaa ctcaccgcac caagttatgt cggtgtattt ggaaaagaca
     5401 tccagaaaac agccaaaatg atggtcaaac ataacctcgt gcaaatggct gcttccgatg
     5461 cccatggggt ctctaaacgt accttttact taaaagaatg ttatgagcaa atcgccaagg
     5521 actttggtaa ggaaaaagta gcacagatga aacaagtcgc aaaggacctg atcaacgggg
     5581 atgagatcag ttatccagtc tatgaagagg taaaaaagaa gaagtttggt ttattttagg
     5641 gggaatgtag ggtgtttgta ttaacaagaa gaagaaaaat tgccacattg gtatttgtgg
     5701 acagcttgtt gttgattgct gcgaatattg cagcagctaa attcatgaag ccgtttgtag
     5761 ctataccaat ggaccttatt ttaatctcta tcggtttgtc gattggtttt tatttattct
     5821 atggtagttt attcaaagtt tttactcgaa tcaatcgtta tacaaattta agagagatca
     5881 ttgcgatttt tggttcgtta tctgcaagtg cggcctcaag tatcatcata ttgcttttca
     5941 taaataaaca atacagttta cgtttagtga tctttgcata cctactttcg ctattactga
     6001 tcattggcag ccgattgatc tggcgtatct atatcgaaac aaaaaatatg aggtatctat
     6061 caacagattc cgctaaaaat accttgatcg ttggggccgg cgaaggtgga cgtattttat
     6121 acaacagctt tcttggttct aaaacggctc aagatatcca tgtcattgga ttcgtggatg
     6181 atgatccgaa taaaagaaat acctatcttt caggaaaaaa agtactgggg tcattaaaag
     6241 atataccgga attgatcgaa aaatatgaca tccaaatggt aacgatcgct atcccttctt
     6301 tatcaagaaa aaaattacga agaatcttcg agttagtcga atctgctcat gtgaaagtca
     6361 atacgatgcc ttctatggaa gaacttgcct cagggaaaat cagcgtatca aaattaaaaa
     6421 ccatcgacgt agtggaatta ctgggaagag atgaggtaga gttggatatc gaatccatca
     6481 aagaccagat cacaaacaaa gtcattcttg tcaccggtgc aggaggatca atcggttcag
     6541 aaatctgccg gcaaatcatc caatttgacc ctgcaaaact attactttta ggtcatgggg
     6601 agaactcgat ttacttgatt gaccgtgaat tacgcacgca tcatcaaaag tgtcctacag
     6661 aaatcgtgcc gatcattgcg gatatccaag atcgcaagaa aatcaatgag atcatggaac
     6721 aataccaccc agatatcgtc taccatgcag ccgctcataa gcatgtacca ttgatggaat
     6781 ataacccaaa agaagcagtc aaaaacaata ttttcggtac aaaaaatgta gcagaagcag
     6841 ccaaagcagc gaaagtaaaa aacttcgtca tggtctcgac agataaagcc aacaatccgc
     6901 caaacgtcat gggttcaaca aaacgaatcg ctgaaatgat cgtgactggg ttgaatgaag
     6961 aaggatgtac gaaattctca gctgtcagat ttgggaatgt ccttggttct cgcggtagtg
     7021 tgattccagt attccgggaa caaattgctc aaggagggcc agtcactgta acagactttc
     7081 gtatgacccg atatttcatg acgattcctg aagcaagtcg tttagttatt caatctgggg
     7141 cgttagcaaa aggcggcgaa atctttattt tagatatgag tgaaccagtg aaaatcgtag
     7201 atctagcaaa aaacatgatc cgattgagcg gatatagcga agacgagatc gaaatcattg
     7261 aaacaggtat ccgtccaggg gaaaaactat atgaagaact attgttagat aaggaaagaa
     7321 acgatgaagc agtttacgag aagatctttg tgggaaacat caaaggttat tccatgcaaa
     7381 cagtgatgga atttgtcaag agcctgccgc aagacgatga acaattggct aaagacattg
     7441 tcacttttgc gaatgcttcg aataagtagg gggagtaaag tgaaagcaat tattgtagga
     7501 gctggtggat ttggcaaaga gattgctttt ctactagaaa gtttctctag atatgaattg
     7561 attggttttg tcgatgattc gttaaaaatg caaaatcaag aattagtggg aaaaactgta
     7621 ttgggaacaa tagactcgtt attagaatta gaagaagaaa ctgctatttt tctgggaatc
     7681 gcttcgccag atatcaaaga gaaaatatat caaaagataa ataaaaataa taaattgatt
     7741 ttccctaatt tagttgcatc aagtgcacta gtgggcataa atgttcagtt aggaattggc
     7801 aatattttga tgccctatac gacttacact gctgacgtag ttttaggaaa tttcaatatg
     7861 atcaatatcg gctctactat cggacatgat actcaaatcg gtaattataa ttc
//
LOCUS       DAXWLF010000124         7789 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXWLF010000124 DAXWLF010000000
VERSION     DAXWLF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.124"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            15..716
                     /locus_tag="ACGE4U_002073"
     CDS             15..716
                     /locus_tag="ACGE4U_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8394196.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1288..2304)
                     /gene="tsaD"
                     /locus_tag="ACGE4U_002074"
     CDS             complement(1288..2304)
                     /gene="tsaD"
                     /locus_tag="ACGE4U_002074"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFF8394197.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2301..2765)
                     /gene="rimI"
                     /locus_tag="ACGE4U_002075"
     CDS             complement(2301..2765)
                     /gene="rimI"
                     /locus_tag="ACGE4U_002075"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFF8394198.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2772..3314)
                     /gene="rimI"
                     /locus_tag="ACGE4U_002076"
     CDS             complement(2772..3314)
                     /gene="rimI"
                     /locus_tag="ACGE4U_002076"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFF8394199.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3298..4020)
                     /gene="tsaB"
                     /locus_tag="ACGE4U_002077"
     CDS             complement(3298..4020)
                     /gene="tsaB"
                     /locus_tag="ACGE4U_002077"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFF8394200.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4211..5191)
                     /locus_tag="ACGE4U_002078"
     CDS             complement(4211..5191)
                     /locus_tag="ACGE4U_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8394201.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5215..6699)
                     /gene="galT"
                     /locus_tag="ACGE4U_002079"
     CDS             complement(5215..6699)
                     /gene="galT"
                     /locus_tag="ACGE4U_002079"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFF8394202.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6711..7700)
                     /gene="galE"
                     /locus_tag="ACGE4U_002080"
     CDS             complement(6711..7700)
                     /gene="galE"
                     /locus_tag="ACGE4U_002080"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFF8394203.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2168 a         1688 c         1381 g         2552 t
ORIGIN      
        1 gagaggactt tttaatgcgt gttaataaaa tcgattttca cttttcaaaa ctcagatatc
       61 aacctgattt tgaacatttc tcggatgaag agttccaaca gatcaaggaa catactgttc
      121 ttcgttctta taaaaaagga cagatccttt tcgacgatgg tgatcccaga caacggttgt
      181 atatcttgac caaaggactc gttcgtttag aacgttatga tgaaagtgca acttattact
      241 actatgacta tgtgaataca aataaccttt ttcctatgtc aggaatgttt ggagaagaga
      301 cctacactta tacagctcaa gcgatgactg atattgaaac attctacctt cctgtttcct
      361 actatgaaaa attagcacga aaaaatcccg aacagttgat ctatatgata caaaaaattt
      421 ctaaagtagt tgaaatacat gaattgcgta tccaaatcgg attgacctct agcgcctttg
      481 accgggtaaa acaaaattta ttcattttga aagaagaatt gggcgtacca acaaaagatg
      541 gtcatatcac cattccttat ccgatcacct taaaagaatt agcagtgaat agcggcacga
      601 cacgtgaaac tgctggacaa gtaatcaaac aactacgtga ttccggactg ttagattatg
      661 acaaaaagcg ttttactttc tgtaagacag ctactactcc agctatgaga gaataacgcg
      721 aacaaaaata agcagtgacc ctgatattta tcagggtcac tgcttatttt cctccctaaa
      781 acttctgaaa gatccggcag ttttagatca ctgggcaaga ctcaaagatt ttctatgtgt
      841 gtttcagtta tgtaatattc actgtttgca aaaaatactg atagattaac tagaggaagc
      901 gtcttgtcct catccgacct agattattcg atattcctcg acaataaacg attaatcagt
      961 gagggcaatc agaggaacgg ataatgatga ataagctgta aaaaataggg ctttgacaaa
     1021 agcgtgtcac agcccttatt tcgattaaac ggtgtgtacc tgtcaactct tcggtcacaa
     1081 gtcataatgt tcataaacat gaggagctgt tttttgaaat tgttccttgt gaatcagagt
     1141 taaacgacag gcttacgaac tcttgattaa acggcgttca aaagcttgca tacgcggtaa
     1201 taagaacagc caggtcaaaa acatgagaag ccgtttttcc tgggctgttc ttattacttg
     1261 atgcagggca gatttttcac aacctcttta ttcagataaa accaagcttg gatcagcatt
     1321 caactgatag ctagcaaaat ttcttttttg catttccaca tgtgctgctg ctccaatcat
     1381 tgcagcatta tctccacaaa gtcgaagagg gggaatcact agttctacct cagggagttt
     1441 agttgccaaa gcatcctgca atccttcacg caacccttga ttggccgcta cacctccagc
     1501 tacaatcagt tgcttgactg gataattttg acaagctcga agtgttttag taatcaatac
     1561 atcgatcaca cttgcttgaa agctagctgc taaatcattt ttgtctagat cttctccacg
     1621 ctgttgtgca ttatgcacca aattgataaa tgcacttttt aatccgctaa aactgaaatc
     1681 ataattatct tcatggatca tcgctcgtgg aaaatggtaa ttatcttttc cctgatgagc
     1741 tagctgatcg atttcttttc cgcttgggta agaaagacct aatactcttc caactttgtc
     1801 ataagcttct cctgctgcgt catctcttgt ctccccaatg atttcatatg atccatcttc
     1861 ttgcatatag actaattctg tatgtcctcc gctaacaaga agtgccatca atggaaactg
     1921 gaatggttta accagacggg ctgcatagat atgccctgcc atatggttta caggaataag
     1981 cggcaattgg tgtgcccaag caaaagcctt agctgcagaa ataccaatca gcaacgagcc
     2041 gacaagtcct ggtccataag tgacagcgac tgccgataaa tcttctgccg acacaccagc
     2101 ttctaccagt gcatcttcca agcataaggt gatttgttct acgtgatggc ggctagctac
     2161 ttcaggaact acgccaccaa aacgtttatg actgtttatt tgagaagcaa cgatgttaga
     2221 taagatctct gttccatttc taacgaccgc tacactcgtt tcatcacaac ttgattcaat
     2281 cgccagaatc aattcttcat tcatttcatc gtctccgttt ttaactttct gctcataatg
     2341 atcgcatctt ccgtcggatt gcgataatat gatttccgtt tacctaatat acgaaacttt
     2401 tctgattcat aaagttttct tgctgcttca ttagatagac gtacttctaa aaaaacatgc
     2461 agcgaatctt ttatttgttc cgtaatcagt aattgtaaca aatgccgtgc atgccctttt
     2521 ccttgttctg aaactgcaac agcaatattt gtgatctcga cttcatctat tactttcgag
     2581 tatccaataa agccacagat ctggttatct tcaactaaaa gcagataatc agagtgaggc
     2641 tgttggatgt ctgccaaaaa ctgcattttc gtccaaggtg aaccatgtgg ataagcattt
     2701 tcgctaatca tccatacgca ttctgccaag tacgcttcat caaaatcttt ttttctgcaa
     2761 atcaactaat ctcactcatt ttctaaaagt tttatcatat ccaacgcatc ttcttttgtt
     2821 tcagtgtaat atcttttttt gatcgttgtt gcttgaaaac ctaacttccg ataaagtctt
     2881 tgtgcatctt gattactcat acgcacttct aatgacatcg tctcacagcg attcattata
     2941 gcaaagttct cgatttcgtc aataagcagc gaaccgattc gtttacgctg atatgccggt
     3001 cgaacggcaa tattcgtgat atggcaatct aaaccgaacg ctcttgaacc gataaaacca
     3061 atcattcttc cattatcaaa gatacctaga tatagatgag gaagcggtga cttcaattct
     3121 gatataaagg cactttttgt ccatggcaat tcgccattat agacttctcg ttcgacatcc
     3181 agcagctctt tgatatcttc tgtagtcaat tctcttagta cgtaacgacg atcgatttgt
     3241 tcgattgttt tttctgtata aatcatttta tcttgaaaaa agcttctggc atacgctcta
     3301 aattttttca acataacttt cgtctccagg tgtgtgcgtc tccaaccatt tttcttctgc
     3361 ttctacccgt tttaaatagc gaggaagaaa agcttgtttc tctgcaactg gttcagctaa
     3421 agcacctaat tgagccacag ttacaccact tggaatatcc catgctggaa ctgtattaat
     3481 catcgcacta ggcaatattt cctttatttc atagaagaat ttgtgacaat cactcccgac
     3541 gaaataaata gcttcattca gcttttgtag ttgtgtgaac aaatcagaag cagaaatatg
     3601 tacatcttca agtacaattt ctaatctatt ttctttccag cggtatgccc cagcgtatac
     3661 attttttctt cgagcatcaa acatgggcac aataatttta tccacaccga tgcaattagc
     3721 cgcgatggtt ttcagactag atactcctac tagctcttta tctaatgtat aagcaatcgt
     3781 ttttgctgtc gttacaccta tacgaagccc tgtataagaa ccgggaccat cagaaaccgc
     3841 gattcggtca atgtcttttg gacttatttg aagatctgca aacaattggt cgattgcagg
     3901 catcagtgtt acactgtggt tgcgtttgat atttgtttgt atttgtccaa gaatttgttc
     3961 gtcttctacc acaccgatcg ccaaagtttg gtttgcagta tctattccta atgtaatcat
     4021 atatttgatc ctttctgcta tgacattctc gccttccatt gtagcatact tttttattct
     4081 gaggatgaaa ataccaaagg caaaactagt acaatgatac atgattatct gctggcgggc
     4141 aaattttgcc ccttcctgaa acacaaaaaa gctgcggtat cacttcattt ccccacagct
     4201 tttccatctt ttatttcgtt gattctcgaa taatcaactc tgtttccaca ataatctttc
     4261 ttgaaaccgg tgctttggaa ttacttaatt gaagaatcgt ctccactgct agctctccca
     4321 tccattcggt ataaactttt acagtagaaa gagagggcgt tacatatttc gctacactga
     4381 catcattgaa acccatcaca gaaatatctt ctggtaccac taaacctttt tcttgaatcg
     4441 ctttcagtgc gccaatcgct aatgcatcat tagctgcaaa aagcgcatca ggaacatgcg
     4501 gattcgtttt taaatactgc ttcactgctt gatatcctga ctcgacagaa aaatctgctt
     4561 cgataattgg accttcgtcg tagccttttt ctgccaaaac ttgttgaaat gccataattc
     4621 tgggatcttt tagccgctga tggtttttct tcgtatattc tgttccagca agcatagcga
     4681 tatgttgcgc tttatgggag acaaaatgtt caatgacttt ttgaacactt gacttaaagt
     4741 cgacaactag agagtcgatt ccttgagaag tgccgtcaga atctaccaaa agcaaggcat
     4801 tttgggatcc tttcatttct aataactgag cttcgtcgaa ttttcctagt gcgatcgttc
     4861 catctgtcgg aagatcacta atggaatccc acgtttcttt caataaagta acgtttgctt
     4921 cttctgcctt tttttcgatt cctagacgaa tagataaata ataaagatct tcaagctctt
     4981 ccgtatcgtc gtaccactgg atcaggcgaa gcgtttgctt agtatttgct gcatttttac
     5041 gatgtttcgt ataatttaag cgttctgctg cttcaaaaat tttttgcttt gtggctaagc
     5101 caacggaaag atcaggatca tagttgagaa ctcttgaaac agtagcagga gaaactcctg
     5161 ccaactgcgc aatgtcttta attgttgcca tttgtctctc ctcctttttt acgtttataa
     5221 aattgcagta aatcgttcaa acgctgcctg tcctttttca tctcgcttga atactccagc
     5281 gtcttccagt acacgtgcaa aaacctgacc tacagatttt tgtacgacat cggtgaccgt
     5341 atctttcgtc aaatcaggat gttctttttt taattgttct gcccacggac gatgatattc
     5401 agccatctgg ttttcttctc ctaataagaa atgctctact tctactagtt ctggtttcaa
     5461 acgaggtggt aaaacagcaa gccccattac ttcgatcaac ccaatgttct cctttttgat
     5521 atgttggaca tctttatgtg gatgaaaaat cccatcagga tgttcttctg aaacattgtt
     5581 gtctcgaagg acaaggtcca actcaaagta attgtccctt cttcgtgcaa ttggcgtaat
     5641 cgtatggtgt ggtgtaccat ctgctgaaaa agctctgata gatactgttt catccgaata
     5701 tgctttccac ttttccaata ctgagttagc tgcttcaatc aacaattttt tctctgttga
     5761 ttgcaaacgg atcacagaca tcggccattt cacgactcct gcgatgactt gcggataatc
     5821 tgctagtttg ataagttttt ctatcggtgc ttttgccata ggaaattcat gacgtcctgc
     5881 ctgataatga tcatgagaaa gaatcgatcc tccaacaatc ggcaagtcag cattcgaacc
     5941 aacaaagtaa tgaggcaaga cttcagtaat ccgaagcagt ctagcaaatg tttcctttgt
     6001 gattttcatc gggcggtgtt gttctgataa aatgatcgaa tgttcattgt aataagcata
     6061 aggagaatat tggaatcccc agctttcacc gtctagattc attcgaataa tccggtgatt
     6121 ggtgcgggct ggatagttca agcgacctct atatccttcg ttttccatgc aaagcatgca
     6181 ggtaggatag tctactttta ccgcctcgcg ttctgctgcg atttgttttg gatctttttc
     6241 tggcttagat aaattgattg tgatttccag ttcaccatac tccgactctg ccggaaattt
     6301 gatgtttttt gcgatggctc ttgttttgat gtagtcattt ttcttactta aattaaagaa
     6361 atactcagtt gcttcctctg ggcttttcga atagtgctgt gcaaagaatg cattgacgac
     6421 tgaaggtggc ggtgtgagaa aatccattaa ttgcgcttca aaaatctctt gttctgctgg
     6481 caattctgaa agaacaccat tttctcttgc cttagcgaca agctgatcca ataagtctac
     6541 agattcttcc ttaactggtc ttacttcaat gctttctaga gcatcttctc ctaccatccc
     6601 taaaagacga ttctgtaaat ataagcggtc catttccatc catccgccag cttcgatcgc
     6661 aagtgtggca aaatcacaaa tcgtttgact gattgacata tctcattccc ctattcttca
     6721 taaccatttg gatggctgac atgccaatcc catgctgttt tgataattgc tttgatatct
     6781 gtgtattcag gtttccaacc cagaacctct ctcgcttttt cacttgaagc gactaatcgg
     6841 ctaggatctc cagctcttct tggtgctatt tctgctggaa tttcttttcc tgtcacttct
     6901 ctagctgctt ctagcatttc cttgactgag tatcctttgc tgctgcctaa attgaagaag
     6961 ttgctttcat ttccttcttt caagtattcc aaagctaaaa tatgtgcagc aatcaaatct
     7021 tctacttgaa cataatctct gatacatgtt ccatctggtg tatcataatc gtctccataa
     7081 acagccaatg cttttctttg accaagagct acttgtaaaa tgattgggac aagatgcgtt
     7141 tctggtgtat gatcttctcc gatagatgca tctgcttttg ctccggctac attgaagtag
     7201 cgcaaagcga catagcgcat gccatatgct tggtcacacc acttcatcat tttttccatc
     7261 atcaatttac tttcaccata cggattttta ggattagctg gtgtatcttc tgtgattgga
     7321 gactcttttg gttctccgta agttgctgca gtagaagaga acacgatttt attcacattg
     7381 aactcgtgca tgacttcaag taagatttgc ataccgtata cgttgttgtt gaaatacatc
     7441 aatggttttt cgacagattc tcctactaaa gagcttgcag caaaatggat tactccttca
     7501 atcggttcct tttcaaatac ggaacgtaga aattctttat cccggatgtc tccttcataa
     7561 aaatgagcat ctggatgtac tgcttgtttg tgacctgtga gcaaattatc taccacagct
     7621 acttgatacc ccttttgaac tagctggtct acagcgtgcg aaccgatata accggctccg
     7681 cctagtacta aaattgtcat aaaaaacact cctttgatta gtaaaattgt gtttgttgat
     7741 tatatataat ttaaaaaaac gaagtttact tttatggtga aaacaaaca
//
LOCUS       DAXWLF010000125         7767 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXWLF010000125 DAXWLF010000000
VERSION     DAXWLF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.125"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(78..150)
                     /locus_tag="ACGE4U_002081"
     tRNA            complement(78..150)
                     /locus_tag="ACGE4U_002081"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="ACGE4U_002082"
     CDS             531..1100
                     /locus_tag="ACGE4U_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFF8394204.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="ACGE4U_002083"
     CDS             1087..1947
                     /locus_tag="ACGE4U_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8394205.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /gene="yycF"
                     /locus_tag="ACGE4U_002084"
     CDS             2137..2841
                     /gene="yycF"
                     /locus_tag="ACGE4U_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFF8394206.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="ACGE4U_002085"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="ACGE4U_002085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFF8394207.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /gene="yycH"
                     /locus_tag="ACGE4U_002086"
     CDS             4678..5994
                     /gene="yycH"
                     /locus_tag="ACGE4U_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFF8394208.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="ACGE4U_002087"
     CDS             5995..6864
                     /locus_tag="ACGE4U_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFF8394209.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="ACGE4U_002088"
     CDS             6919..7728
                     /locus_tag="ACGE4U_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8394210.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
BASE COUNT         2652 a         1316 c         1696 g         2103 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaa
//
LOCUS       DAXWLF010000126         7694 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXWLF010000126 DAXWLF010000000
VERSION     DAXWLF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.126"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            543..1106
                     /locus_tag="ACGE4U_002089"
     CDS             543..1106
                     /locus_tag="ACGE4U_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8394211.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1385..1672)
                     /locus_tag="ACGE4U_002090"
     CDS             complement(1385..1672)
                     /locus_tag="ACGE4U_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8394212.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(1662..1991)
                     /locus_tag="ACGE4U_002091"
     CDS             complement(1662..1991)
                     /locus_tag="ACGE4U_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8394213.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2296..2625
                     /locus_tag="ACGE4U_002092"
     CDS             2296..2625
                     /locus_tag="ACGE4U_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFF8394214.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2912..3397)
                     /gene="vanZ-A"
                     /locus_tag="ACGE4U_002093"
     CDS             complement(2912..3397)
                     /gene="vanZ-A"
                     /locus_tag="ACGE4U_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFF8394215.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3550..4461)
                     /gene="vanY"
                     /locus_tag="ACGE4U_002094"
     CDS             complement(3550..4461)
                     /gene="vanY"
                     /locus_tag="ACGE4U_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFF8394216.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4889..5497)
                     /locus_tag="ACGE4U_002095"
     CDS             complement(4889..5497)
                     /locus_tag="ACGE4U_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFF8394217.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5503..6534)
                     /gene="vanA"
                     /locus_tag="ACGE4U_002096"
     CDS             complement(5503..6534)
                     /gene="vanA"
                     /locus_tag="ACGE4U_002096"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFF8394218.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6527..7495)
                     /locus_tag="ACGE4U_002097"
     CDS             complement(6527..7495)
                     /locus_tag="ACGE4U_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFF8394219.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2349 a         1532 c         1280 g         2533 t
ORIGIN      
        1 taaaactttt ttagcagatt ttaaagtttc gtcaaaacta atatattctg gtagctgagc
       61 attcaaatag ctttcagact tcagaagaaa attacgagca tttttatagt ccatctctaa
      121 taaagttatt ctttttttat ccaatacatt cagctcaatt ccttataata ttctattcct
      181 tagtatctca acattattta ctttataaaa cgaataaatt ttgtagttct aattcaaata
      241 ttgcatgtta tatctaatat gggagcattt tataattttc attgtgataa actcgttatt
      301 aactttagga tacactagtt aggattaaat taggtatcta gaaatttact taagattttt
      361 ttgtttattt cgtatacaca ctcgtatcaa aaacaatcga ttatgagaca aaatgaaatg
      421 acctctttta ttaaataaaa gaggtcatat tttgtatcat aacttgttgc attaactatg
      481 tatctataac tcccttttat gatacaatca agccagaatt aaagtacgaa aggaagaaaa
      541 gcatgagtgg atacatttat ggttatgccc gagtgagtac acgttcacaa gaactaaatc
      601 gtcaattaga cttactcaat gaacaaaatt gtaatgaaat cctaactgag aaaatgacgg
      661 gaaccaaagc ggttcgtccc caactaaatc gtttaaaaga taaaattcgt ccgggagata
      721 cggtaattgt agaaagtttt tctcgattgg gtcgtagtac aaaggatttg attgaccttg
      781 ttacctattt tgaagaacac catgttaagc tggtgagctt gaaagaaaac ttcgatacca
      841 gcacgcctca cggacgctta atgatgactg tcttccaagc atttagtcag ttcgaacgtg
      901 atttaattgt tgaacgtacc aaagaaggtt tgaaaagtgc acgtgcgaga ggtcgtaaag
      961 gtggccgtcc acgagtgaac cagaaagatg ttgatcgagc agtaaaattg tataaaagtc
     1021 aggtttatag cgtaaaagaa attacggaaa tgactggtat tagcaaggca acactctacc
     1081 gctacttaaa agacaatagg gaataatcta ttttaatctt taggaatcta taatatttat
     1141 gggagaatta gagtgatttt tttattatta ctaaaaaaca atataaaata acgcatttta
     1201 agccctcttt gtgcataagg atgaagcttt taatttgaaa gaggcttaaa atgcgtttta
     1261 attttttcta tttttcataa atatataggt tagtggttta aaattatagg gtattacaac
     1321 tctcagctgt taaaaattat cttttaaagt gtcgtctatc ctctcaagtt gaaacattgg
     1381 gttcttacct aatctttttt aattgttcat aaaaattttc atgcgttcca catagtaagt
     1441 taggtatcac tgcattatct ttaatttcat atgcaacgcg atatgtcgta cctgcatact
     1501 tgaatccaat agcccaaatt cctgataaat cacccgtttt ttgttctccc gaatgaggac
     1561 gaactgctat ctcatcataa attatcgtta aaaattgttc ttttaatgtt ttctctttta
     1621 atttttttag atactttatt acgtgtttag aaggtagaat ctcataagcc aatttctgct
     1681 gccaactcct cttttgtcat cggtttagaa tttgcaacag tatcttcagc aatagattta
     1741 aatgcagaag ttaacgcatt ttttctattt ctaaattctt ttaagatctc ttgtttgcta
     1801 taaccttctg aaagaatatc agccaaaata tcttcactaa aatcaaaaaa ttctttttta
     1861 ggctctacaa actcataaag gataccattc tctacaagct tagcttctac ttcaacacca
     1921 ttgggcacat cgaattcttt agggaccgtt aacataatag aatttccttg ttttcttgtt
     1981 tttgtaatca tattatttcc tccattattt tacatttcgt aattacttag taattacatt
     2041 gtacgctatt tttagtagaa atacaaaata ataaaactcc tttagaacaa ttttcaaagg
     2101 agttttattt gattaggaaa ttatgtagaa taataatatg tttttaactt ggaatcaaaa
     2161 acgcacttgc aagtaagttt acttcaatac atgaaattgc ttataatagt gtattggaat
     2221 taaggtagat tgacaaaaga gtgatcaaat aggtatactt cattcaaata atcgtttgaa
     2281 tgtgaggtga gatgaatgaa aaaagtttgc caagtgactg tcgtacataa agaaaaagta
     2341 aatggaataa aaaataaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa
     2401 tgtttcagcg atttttctag aattaaaatt ttctatgctt tagaaaccta taaggaaatg
     2461 tgcgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta
     2521 cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattac
     2581 tctttagcga atgaagacat cctttccgct attcgtgttt tttaaagtta tcagaaaatc
     2641 tatctatgaa atcttagttt gggggtaccg tcaggaaaat gcggatttac aacgttaagg
     2701 aaatttatta aagagaccaa ttcctcagca ctaaaattga taattgggct ttctttgtta
     2761 ctatagtgtt aggtaagctt tttactttat caccggtttt cccctcactt cacacctacg
     2821 ggcgagtttc cccacatccc gttttccatc taatccaatt cattcaatat ctttatgtgt
     2881 atgaaagatc agattgcttg atttagacat cttacacgta atttattctt aaatgggtac
     2941 ggtaaacgag caataatacg agcaaaagta tacctacaaa aataataact ctgtctaatt
     3001 ttttttgatt catatgctta ttgcttaaac catataattt cagtccaaga aagcctccaa
     3061 cagtatttgt aattacatct gttatgtctg tcgctccaat agcgaagata aattgaatta
     3121 tttcaaaagt aagacttaaa accagtacaa aagcaaactt aggtaaaaat ccgatttctt
     3181 taaaattgac attcaaaagc aagccaaatg gaataaagat tataacatta tctatcatct
     3241 ctctgaaatt cccagtagca gtaaatggag tcaagttaag acttctttga tgataattaa
     3301 atactgataa aatattgtat tgtaatttga ataacactaa ccagattagt gtcactaaat
     3361 ataaagctag caatcctcta gataatattt ttcccaatat aatttcctct aattcttttg
     3421 tttaagttgt atcattagta tagcaatgtc ctagttaacg tgggttttaa attaaaaagc
     3481 taacgttacc ccaaaagtgc tagtacaata atgctgttca ttaatttcgt tggtttcatt
     3541 ccgccatcct tacctccttg aattagtatg tgttgatccg ggaaacactg tcacaattac
     3601 accgtctata ttgtttcctg atatctcata acgaagatta gtcggcacat gaatggtggt
     3661 atttttagta acaggataat aaaagatctc atatttttcc ccatttacac taacagaaat
     3721 ggttttttct tcttttaggt aatccatata ttcctcgaga acgaaattct tttctttcat
     3781 aatcgcacta tgtggtaaac caacatagcg aatatgccat agttcatatt gaattcctgt
     3841 taactctgtt ttgtcctctg gataacgtaa aatgaacccg tatttccaag cattttcttc
     3901 tatccacttt ccttcagggg ctcgttccat tttcgtcaag cttgatccta catctagtga
     3961 taaacctgaa ttatgctcac tataacctgc tggtaaggca tactcagccc ccatttcttg
     4021 gtaaagcaca ctttgctcat caaagtctcg atagccacta ttaataataa aatgactaac
     4081 gccacccttt acagcatcat tgaccatctc tgaaaatttt tgtgctattt cttttgacat
     4141 ataaatatta ctatcaagca acccgtatcc atttattaat tcgtcatgtt tagataaatt
     4201 cacgatatct gacttcacac tttcttggcg aacaggatat ttactattga ttaatagcag
     4261 atttccttga taaacctgtt cttctgtaat tgttttctct tgggtatttt cagaagtccc
     4321 actattttct aaatgttctt tgggattttg atcataattt tgaaattcga ctttttcctg
     4381 agaaaacagt gcttcattaa cgtagtcata acctaagtat attaagaata acaataacaa
     4441 taaaaaaaac aacttcttca ttttcagtct cctccttaac tatatagttg gaagtatgaa
     4501 atatttctag aatcttcccc ttaaacggca caatctcgtt tgagattgtg ccgttttgcc
     4561 ccgtaaatta cagtcaatta tttcgcccta ctggatttac tattatcacc aatgtagatg
     4621 aattttcata agtgataccc atgctgaatt taacgaggct gaactgcctg ttgacaatgg
     4681 gcggtgtgaa ataccacccc tctatgcgat cagtcttatt atcaggctat cttgccgcag
     4741 cgcacgcaga taatgaccgc aatatcgctc tgccttacat cttcaatact atctactata
     4801 tataaattcc ctaagaacca gttacatacg tcgggtttcc tgccgaaaga gttagcttat
     4861 atagtttgtc cgtgcaacgg ttaaaagttt atttaacggg gaaatcaaaa tagctattgg
     4921 ggtatggttc gtctcttaat acatagtgcc accattcgag gctatatgct tcaaacccac
     4981 tgttttccat gatggagcgc aaacgtctgc gattttgcgc ttcattgcat gatattccat
     5041 ttgccgcatg atgagagcgt tcatccataa aatcaaatcg gctccccatt ggtacaagct
     5101 cacccgtgtc taatcgataa agcgtaagat caatggcact gccgcggcta tggcttgatt
     5161 ttgaagccac gtatcctttt gaaatcatct cagttcggtc aatattggga taataacttt
     5221 cctttgtcag gttattttcc ggctgtgcag cccattgcat aaaacagttt acagcacgat
     5281 taggacggta accgtcccat agaagcaatc cgtacccttg ggtagcagcc agttcttttg
     5341 ccttcaaaag cgattcagcc aactcgtatg tccctacaat gcgatttact tcataaccgt
     5401 caaccggttt tccggtgaaa ttatcccaag tggcatattt agcgtcccaa cgaacaccgt
     5461 gtactatttc atctaaaaaa gtaaatccta tttccatgct tatcacccct ttaacgctaa
     5521 tacgatcaag cggtcaatca gttcgggaag tgcaatacct gcagcggcca tcatacgggg
     5581 ataacgactg tatgacgtga aaccgggcag agtattgact tcgttcagta caatgcggcc
     5641 gttatcttgt aaaaacatat ccacacgggc tagacctcta cagccgagcg ctttatatat
     5701 tttttttgcc gtttcctgta tccgtcctcg ctcctctgct gaaaggtctg cgggaacggt
     5761 tataactgcg ttttcagagc ctttttccgg ctcgacttcc tgatgaatac gaaagattcc
     5821 gtactgcagc ctgatttggt ccacctcgcc aacagctaac gcggcactgt ttcccaatac
     5881 cgcacaaccg acctcacagc ccgaaacagc ctgctcaatt aagattttgc tgtcatattg
     5941 tcttgccgat tcaattgcgt agtccaattc gtccgcgcta ttgacttttt tcacaccgaa
     6001 ggatgagcct gaacgcgccg gcttaacaaa aacaggatag gtaaacgtag ctgccaccgg
     6061 cctatcatct ttattaataa cccaaaaggc gggagtagct atcccagcat ttttcgcaac
     6121 gatgtatgtc aacgatttgt ccatacaaat tgctgagctt tgaatatcgc agcctacaaa
     6181 agggataccg gacaattcaa acagaccttg tatggatcca tcttcacctg acttgccatg
     6241 caaagctgaa aatgctacat caacatggtt gatttcatat tcatggttct ttttaacaag
     6301 taatccgtgc atttttttat ccggcgagag tacagctgaa tagcaattgt cgttttccca
     6361 ttccgcgcaa ggtttttcgc acattttcca tacaccagat ttcgtaattc caatgtataa
     6421 cggctcgtat ttttctttat taatgttagc ggctatctct attgcagatt ttaccgatac
     6481 gtcatgctcc tctgagcaac ccccaaacag tattgcaact tttattctat tcatgctcct
     6541 gtctcctttc aaaatccaaa cagtttttaa tggttttttc aacggtatca cgcaacgctt
     6601 gctcggtata ataggccgta tgcggtgtga ttatcacgtt aggcattctt tgaagtttaa
     6661 gtaaaaattg attatcaatt ggtttttggg tgcaatcaga gtagaaaaac tcttcctctc
     6721 cttccaatac atccaatgcg gcaccgccca gtttcccgtt ttctaatgct ttaaccaact
     6781 cataggtatc tacaagtgga ccgcgcccag tattgataag aaatgctcct tgcttcattc
     6841 tctgtatttg ttcgtggctg ataatatagt gcgtatccgt attgagcggc acatgaagcg
     6901 taacgatatc gctattttgc agcaactcat caaacggtac atagtttacc tctatacttc
     6961 ggctgcgact ataagccaac actttacatc caaatcctcg cagccgctca ataaccgctt
     7021 tgcctatctg gcccgttccc accacaccaa ctgtcatgtc gctgagtacc ttgccacggt
     7081 cgctgtccaa cctgaaatca tgtttttcca cagagcgcac aatcgatttt acgttgcgta
     7141 ctgccataag aattagcatc atagtataat cggcaacgct atccggcgag tacgccacat
     7201 tgtcgacagt gatgcccatt ctcttagcag cagttgtatc tatatgattg cagccgatgc
     7261 ttcgggtaga aatatatttc acaccggctc tcttcagcgc aagaagaata gaggcggaaa
     7321 tctctgattt atgtcccaca ctgatacatt gattgaaagg cgcggatttg gcgttggatt
     7381 ccgacacgtt ggcgttaatt atcgttgcca taacgccaaa gcgaggcgaa agagcatgga
     7441 atgcatctgc ctcatcctgc tcacatccat aaacagtaat gccgatgtta ttcataatta
     7501 agaccaaccc tttctgtgaa aggcaccaaa taaaacgggg atgccaatgg taatcttgtc
     7561 aaatctttct ctgaagaacg aaaacggctc gttcttacgg tattaagtct accgtgtaag
     7621 aacgagccgt ttcttaatat taaattgtta atttcctaag aaaaagtaaa gataaccttg
     7681 agattttcct aaaa
//
LOCUS       DAXWLF010000127         7687 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXWLF010000127 DAXWLF010000000
VERSION     DAXWLF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.127"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            22..759
                     /locus_tag="ACGE4U_002098"
     CDS             22..759
                     /locus_tag="ACGE4U_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8394220.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            773..1588
                     /locus_tag="ACGE4U_002099"
     CDS             773..1588
                     /locus_tag="ACGE4U_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8394221.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1601..2248
                     /locus_tag="ACGE4U_002100"
     CDS             1601..2248
                     /locus_tag="ACGE4U_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8394222.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2264..2920
                     /locus_tag="ACGE4U_002101"
     CDS             2264..2920
                     /locus_tag="ACGE4U_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8394223.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3082..3903
                     /gene="racE"
                     /locus_tag="ACGE4U_002102"
     CDS             3082..3903
                     /gene="racE"
                     /locus_tag="ACGE4U_002102"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFF8394224.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3906..5261
                     /gene="rph"
                     /locus_tag="ACGE4U_002103"
     CDS             3906..5261
                     /gene="rph"
                     /locus_tag="ACGE4U_002103"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFF8394225.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5262..5780
                     /locus_tag="ACGE4U_002104"
     CDS             5262..5780
                     /locus_tag="ACGE4U_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8394226.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5887..6387
                     /gene="cbpB"
                     /locus_tag="ACGE4U_002105"
     CDS             5887..6387
                     /gene="cbpB"
                     /locus_tag="ACGE4U_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFF8394227.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6667..7587)
                     /locus_tag="ACGE4U_002106"
     CDS             complement(6667..7587)
                     /locus_tag="ACGE4U_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFF8394228.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         2511 a         1328 c         1662 g         2186 t
ORIGIN      
        1 taataatggg agtgtaattc tatggcaatg attaaatttg atcacgtgga taaatattat
       61 ggcaagttcc acgcattaaa aaatatcaat ctcgaattcg agaaaggaga agttgtcgtt
      121 gtcattggtc cttctggttc aggaaagagc acgatgcttc gctgtatcaa tggattagag
      181 acgatctctt caggtaagct gctgattaat gatactgact tgcatgataa aaagacaaaa
      241 ctgacagaag tccgaaaaaa catcggcatg gttttccaac atttcaattt gtacccaaat
      301 aaaactgttc tggaaaacat caaattagca ccaatcaaag tgttgaagca agaccaggca
      361 actgcagtca aaaacgcgga gaaatttttg aaaactgtca atatgttaga taagaaagat
      421 tcttatcctt ccatgttatc cgggggacaa cagcaacggg tggcaatcgc cagaggttta
      481 gcgatgaatc ctgagatgct gctgtttgat gaacctactt ctgctttgga tccggaaatg
      541 attggtgatg tattagacgt tatgaaaaaa ttggctcatg acggtatgtc aatgattgtc
      601 gtgacacatg aaatgggctt tgccaaagaa gtggcggatc gtgttatctt catggctgat
      661 ggagaagtat tggaagacag cagaaatgtg agagactttt tcgaaaatcc aaatgaagtc
      721 cgagcacagc aatttatcag taaagttatc aatcattgat aggaggaaaa agatgcgcag
      781 gttaaaaaaa atacgcctga ttttcctttt aggcattgct tttcttgcac tagccggatg
      841 taaaggaaat gggttggcaa atgaagatat tttagagcga agcaaatcta ccaatgaaat
      901 catctggggt gtcaagtatg atactcgatt atttggaatg atggatattg agagcagaac
      961 tgtacaagga tttgatgtag atattgcaaa agcaatcact aaaaagatcc ttggtgacaa
     1021 tggaaaaaca gaatttgttg aagtcacttc taaaactcgg attccattat tgaaaaacgg
     1081 aaatatcgat gcaattattg caacgatgac gatcacagat gaacgaaaaa aacaggttga
     1141 cttttcagat gtctatttcg atgctggcca agctttgttg gtaaagaaag gaagccaaat
     1201 caaaagtgta gatgatttga atgcttctac aacggttcta gcagtcaaag gttctacatc
     1261 ggctgcgaat atccgacagc atgcaccaga tgcaaaaatt ttagaattag aaaactatgc
     1321 agaagcattt actgctttac aatctggtca gggagacgcg atgactacgg ataatgcgat
     1381 tttgttaggg attgcagatg aaaatccaga gtatgagtta gttggtggaa cgtttactaa
     1441 tgagccttac gggattgcga tcaacaaagg gcaagaaaac ttcttgaaag ctgttaacca
     1501 agcgctagag gaaatgcatg cagatggcac atacgataaa atctatcaaa aatggtttcc
     1561 aaatgaaact gaaggaaaag tagagtaagg gggagaggca atgagcgaat tgatacaaac
     1621 ttatggacca atctttgtcg acgggttcaa gatcacattg ttttcgagtg tcttagcact
     1681 tgtgtttagt ttgattatcg gaacgttaat ggcaatcttc caattatcaa agaataaagt
     1741 agtgagcgcc ttagcaaagg catatgtaga attttttaga aacattccat tattaatcat
     1801 cgtgatgttt ttttatgttg tcgtaccgat ttattggttt aatctagatg gattccaagc
     1861 aggtacgatt ggattaacga tctatacatc tgcttttatt gctgaaacag tacgttcagg
     1921 gattcaaacc gtaccaaaag gtcaaacaga agcaggacta tcttctggtt ttacctattc
     1981 agaaatcatg cgttatattg ttttaccgca agcattcaaa atcgttatcc cgccattagg
     2041 caaccaattt atcaacctcg taaaaaactc atctatttta gcaatcgtag ctggtttaga
     2101 tttgatgtat caaggagact tgatcgctag tgaaacattc aataccttcg atacgtatat
     2161 catcgttggg ttattttact tagttatcac gctaccgtta tcttacttaa tggtttatct
     2221 tgagaaaaaa tgggcggttc gttcatagaa ggaggggaaa tacatggatt ttagcggcgc
     2281 tttttcatgg atgaatatcc gttttttatt ggaaggatta aaggttactg ttgaagtttc
     2341 attgttttcg atcattttta gtttcttgat cggtggtctg acaggtgtga ttcgttttgc
     2401 gaatatccct ttcttttcca aaatccttgg tgtagtgatt gatattatac ggaatcttcc
     2461 attattattg atcattttct tcacttactt tgccttacct caaatcggca ttcagatgaa
     2521 catcttctgg tccgctgtcg cagcattaac gatttttgag tcagctatgt tgtcggaaat
     2581 tttccgagct ggactcaatg ctgttccaaa aggacagatg gaagcaggat tatcaacggg
     2641 actgacttat gtagaatcga tgcgaacgat cgtgttaccg caagcattta aatcaatgat
     2701 cccggcaatt gtgagccaat tgatttcact gatcaaagat acttcattag cagtaatcat
     2761 ttctttacct gaactttctc atcaggcacg tattatttat ggacagaaca ccaattatgt
     2821 gcttccgatg ttcatcatca tgacacttat gtacttcgtg gtatgttacg cgctttctct
     2881 gctttccggt tacttggaaa agagacggta cagttattaa tagatgattc ttttagcggt
     2941 tgtggcaaac taaagtcaca accgcttttc ctctttccat tcgaaaaaaa gagatttcga
     3001 ttgttttaag caagtaagag cacttgaaaa ttcacgctaa aaagtacaca atagggagga
     3061 tagaatttat tgaaaaaaag gatgatacga ttgacagata atcgccctat cggatttatt
     3121 gattcaggtg tcggcggctt gactgtagta aaagaagccc tgaaacaatt accgaatgaa
     3181 aatattttat ttgtaggaga cacagcacgc tgcccatatg gccctagacc cgcggaacag
     3241 gtaatccagt atacttggga aatgacggat tatctggtgg agcaaggaat caagatgctg
     3301 gtgatcgcct gcaataccgc aactgcggtg gctttagaag aaatcaaagc tgctctttct
     3361 attccagtca tcggtgtgat ccttcccggt actagagcgg cagtaaaaaa aacacaaaat
     3421 aaacaagttg gcattatcgg tacgattggt acggtaaaaa gtcaagctta tgaaaaagca
     3481 ctgaaagaga aagtaccaga attgactgtg acaagtcttg cttgtcccaa atttgtttca
     3541 gttgtcgaaa gtaatgaata ccattcatcg gtggcgaaaa aaattgtggc agaaacatta
     3601 gctcctttaa ccactaaaaa aatcgataca ttgattttgg gatgcaccca ttatccatta
     3661 ttacgcccca tcattcaaaa tgtaatggga gaaaatgttc aactgatcga ttctggagca
     3721 gaaacagtag gtgaagtatc tatgctgtta gattatttca atttgagcaa ttcaccgcaa
     3781 aatggtcgga cattatgcca gttttataca actggctctg ccaaactttt cgaggaaata
     3841 gctgaagact ggcttggaat cggacactta aatgtagaac atatcgaatt gggaggaaaa
     3901 taaggatgcg tcatgacggg agaaaagccc aagagcttcg caaactggag cttcaaacaa
     3961 atgttttcaa atatccagaa ggatcggtag tgatccgttt tggtgataca acagttattt
     4021 gttctgcaac aatagaaaat tctgttcccc cttttttgag agaaacagga acaggatggg
     4081 tgacagcaga atatagtatg ctgcctcgag caacgagtac gagaaatcgt agagaaagca
     4141 gcaaagggaa actttctggt agaacaatgg aaatccagcg tcttattggt cgttccttgc
     4201 gtgcagtagt agacttggaa aaattaggag aacgtagtat catcgtagac tgcgatgtga
     4261 tccaagcgga tggcggtact agaacagcaa gtatcactgg ggcatttgtg gcattgaaat
     4321 tagccattga aaaactttta agagaaaaag aattgtcaga agatccaata aaagaacatc
     4381 ttgctgcagt tagcgtagga atcttaccag atgggacttg cgtcacagat ttggattacc
     4441 aagaagattc cgcagcactt gtagacatga atcttgtaat gacagaatct ggaaaattcg
     4501 tggagattca aggaactgga gaagaagcaa cgtttgatgg tgaacagttg aatgaaatgc
     4561 tcttttttgg aaaaaacgcg attgaagatt tgatcaaaga gcaaaaacat gctttgctaa
     4621 cagagtttgc tcaaaatgat gagcggatag aagaaacaaa aacaatcatc attgcgacaa
     4681 gaaatccagg aaaagcagaa gagtttcgaa atatgttcaa agaagcggga tatcatgtaa
     4741 aaacattact tgactatcct gaattgccag atgtggaaga aactggcagc acatttgaag
     4801 aaaatgcccg attaaaagca gaaacgattg cacaattgct tgatcagcca gtcttagcag
     4861 atgattcagg tttgaaagtg gatgctttag gcggtatgcc aggaatctat tctgcacgtt
     4921 ttgcagggga acaaaaaagt gatgcgggga ataacgccaa attattatat gaattgacag
     4981 atgtgccgga tgaaaaaaga acagcacaat tccattgtac gttagtattc gcggcaccca
     5041 aaaaagacag ccttgtagta gaagcggaat ggcccggtcg agtagcccgt ataccatctg
     5101 gcgaaaatgg ctttgggtat gatcctttat ttatcccaga aggtaagaaa caaacagcag
     5161 cagaattatc gagcgaagaa aaaaacaaga tcagtcatcg ggctcaagca atgaaaaaat
     5221 tgagtgccga gtggaaacaa tggctggaag gagagagata gatgcggtat ttagtagtaa
     5281 gtgataatca tggagacaga gatatcctcg ttgacttagt agatcgttat cgttctgaag
     5341 tggatatctt tttccattgt ggtgattcgg aattagaagc ttctgaccca ttatgggata
     5401 cattccaagt ggttcaaggt aattgtgatt atggaccagg attcgaacag aaaaaagtga
     5461 tccaaactgg tcaggataca gtattcatga ctcacggtca tttatcgaat gtccgatttg
     5521 gtttgacaca gttagcaatc gaagctcgag ctgcaactgc cgatatggtg ttttttggtc
     5581 atacccatca aataggctgt gagatggatc agaaaatcct gtttttgaat cctggtagca
     5641 tttctcagcc tagaggcccg atccaaatcc catcatatgc tcttatcgaa agtttagatg
     5701 accaaatttc tgttcaatac tataaccgtg cccatcaggc agttgaagag ttggcctttt
     5761 ctttttcgag agagcgctga ataaaaaaac tctaattttt aataaaaata agctttattt
     5821 tatgaatttt gcatccggtt tcggtaaaat agtagtgttg aatgaaatag aaatggaggc
     5881 catcagatga ttggacctat agtaagagaa atgctgctgg aaaatcagga aacgtttttg
     5941 attcctgcag aaaatgtggc aaatgttatg tatcatcatc cattgtccca tggtttactt
     6001 gtgttatcga aggtcggtta ttccaaaatt cctgtattag gaaaagatga tcactttgta
     6061 ggactagtaa gtttatcaga tgtggtcaat aaaatgattg acttgcaaac aatcagcatg
     6121 gacccgttag aaggactaac ggttgctgat gtgacagaaa cagatgttcc gacaattgat
     6181 gaaaattggg aactagaaga agtactccat ttgttagtcg attcgccttt tttaccggtt
     6241 gtcacagaag atcggatttt caaaggaatc attacaagaa aagagctgct taaagccgta
     6301 aacttcatgg tgcatgaatt agaacgtcgt aatatattgt ctccgaaaat cgaagaatta
     6361 aaagaacaaa tcgatatcgt gagctaaaga acgagaaact aaaagataat caaaaaataa
     6421 gtagaaaaaa cagcgtacca gttttgactt tatcttgaaa aagaggctgg gacaaaagcg
     6481 tttaactcca agaaataaga aggaattcac gaaaattgct tttcaaattt ttgtgaattt
     6541 cagcttattt ccgaaggagt tgcttctgct tccgccgttt atacgtgttt agagcgtggg
     6601 acaaaagtgt tctttacttt tgtcccacgc tctttttagt gagattcgtt gcatcgtttg
     6661 tccaaattat tttccgacag caattggact gttcggaatc gtgaaaccag aagctgtcaa
     6721 ttctttgaca taggagctca agaactcttc ttttaaggca tattgttttc cgttcaatgc
     6781 gtagcagacc gtacgaatcg caaaattgct gtttccaaga tccactaatc caaagataga
     6841 aggttcagtt tggagctctt cttggtattt ttcagctaat gtacgattta ctcgatcaat
     6901 gatctcatag attttgtcat atccttcttc aggaacaatc cgaatatcta aaagtacttg
     6961 catatttgca cgagagagat tgctgatcgt ggtgatgttt cgattcggga tgaaatggac
     7021 agtaccatcc gttgctttca gctgaagggt acggatacca acagatacga cggttccttc
     7081 aatcgttaag tttgctagct tgatgtagtc cccaacatcc atctgttgtt ccatgataat
     7141 aaaaaatccg gtaataacat cactcatgaa cccttgggca ccaagaccga ttgccacacc
     7201 tgcaattccg gcaccagcca aaagtgatcc gatcggcaca ccgatagcag ataaaattgc
     7261 atagataaaa aagaaaccaa gggtataatg aaaaatagta tttaaaagac tatgtaaagt
     7321 ctttaagcgg ctctcgttta gaatggcttt tttagaatac tgattaaaag acttatcaat
     7381 cagatatttt cccacacggt gaagaatcca aaaaaggacg atcaaaaaaa tcagatataa
     7441 acttttttgg ataaaggcag ccaaaatggc gtcccaatca atcccgtccc aaaaccgctg
     7501 aaacgcattt actttgctga cagcttgatc tgtcaatgtc tcgccagaag agtctgtcgt
     7561 accggcagtt gctaataatg aaaacataaa atcctctttt ctaaaatttg attctttctg
     7621 tattgtacca agaagttttc aaaaaaggaa atagcaaaaa taactataat gaaggaaaaa
     7681 agctgaa
//
LOCUS       DAXWLF010000128         7469 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXWLF010000128 DAXWLF010000000
VERSION     DAXWLF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.128"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            111..1136
                     /locus_tag="ACGE4U_002107"
     CDS             111..1136
                     /locus_tag="ACGE4U_002107"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFF8394229.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            1311..2555
                     /gene="glyA"
                     /locus_tag="ACGE4U_002108"
     CDS             1311..2555
                     /gene="glyA"
                     /locus_tag="ACGE4U_002108"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFF8394230.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            2708..3655
                     /locus_tag="ACGE4U_002109"
     CDS             2708..3655
                     /locus_tag="ACGE4U_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFF8394231.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            3789..4418
                     /gene="upp"
                     /locus_tag="ACGE4U_002110"
     CDS             3789..4418
                     /gene="upp"
                     /locus_tag="ACGE4U_002110"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFF8394232.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(4557..4799)
                     /locus_tag="ACGE4U_002111"
     CDS             complement(4557..4799)
                     /locus_tag="ACGE4U_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394233.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            4920..4992
                     /locus_tag="ACGE4U_002112"
     tRNA            4920..4992
                     /locus_tag="ACGE4U_002112"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4953..4955,aa:Thr,seq:cgt)
     gene            complement(5111..5749)
                     /locus_tag="ACGE4U_002113"
     CDS             complement(5111..5749)
                     /locus_tag="ACGE4U_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFF8394234.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(5872..6504)
                     /locus_tag="ACGE4U_002114"
     CDS             complement(5872..6504)
                     /locus_tag="ACGE4U_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFF8394235.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            6855..7154
                     /locus_tag="ACGE4U_002115"
     CDS             6855..7154
                     /locus_tag="ACGE4U_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFF8394236.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         2575 a         1248 c         1469 g         2177 t
ORIGIN      
        1 atcaacaagt ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg
       61 ataaactcag acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga
      121 aacgatatga tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg
      181 ttgcttttcc taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg
      241 tcaagaaagt gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg
      301 cctcttttga acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg
      361 tgaagaactt ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc
      421 cctcagttgt gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa
      481 cactagaagt aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg
      541 gaaaaccaag tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg
      601 ggattattga tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg
      661 atccaacagc catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag
      721 cagtcatcga atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa
      781 aagcccctgg gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag
      841 atggcgattg gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca
      901 tagcaagccc cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata
      961 acgacaactc agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg
     1021 aaccaacact tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga
     1081 atgcttacat gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa
     1141 ttggttattg tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt
     1201 gaacgaaaaa ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt
     1261 ttttgatggg aaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt
     1321 acaaaacgtt tgaccctgat ctttgggctg cgatcgccaa agaagaagaa agacaagaac
     1381 ataatttgga gctgattgcc tctgaaaact ttgtctccga agcagtgatg gctgcacaag
     1441 gaagtatctt gacaaataaa tacgcagaag gttatccagg gcatcgctat tatggtgggt
     1501 gtgaatttgt cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa ttattcggag
     1561 cgaaatttgc gaacgtccag cctcactccg gctctcaagc caacacggct gcttatctcg
     1621 ctttggtaga accaggggac acgatcttag gaatggatct ttccgcaggc ggacatctaa
     1681 cacatggatc accagtcaac ttcagtggca aaacctatca ctttgttgct tacggtgttg
     1741 atccgacaac agaagttatt gattataatg tggtacgtat tttagctaga aaacatcaac
     1801 caaaattgat cgtagcaggt gccagtgctt acggacgaac gatcgacttt gcgaaattca
     1861 gagaaatcgc agatgaagta ggcgcaaaac tgatggtaga tatggcacat atcgcaggat
     1921 tagtagcagc aggacttcat cctaatccag ttccttatgc agatatcaca acgactacca
     1981 ctcataaaac attgcgtggc cctcgaggcg gcatgatttt gacaaacgat gaagcattag
     2041 caaaaaaaat aaatagtgcg gtatttcctg gtattcaagg cggccctcta gagcatgtga
     2101 ttgcgggtaa agcagtagca ttcaaagaag cattagatcc tgcatttaaa gaatatagtg
     2161 aacaaatcat cgccaatgca aaagcaatgg tcaaagtttt caatcaagca attggaacca
     2221 gagtcatttc aggggcgaca gataatcatc tgatgttgat cgatgttcgc gagttaggta
     2281 taaatggaaa agaagcagag agcattttgg acagtgtgaa tatcactgta aataaaaact
     2341 cgattccttt tgaaacacta agtccattca aaacaagcgg tattcgaatc ggcacgccag
     2401 caatcactac tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa ctagttgtca
     2461 aagcattaca agcaaaagat gataacgctc aactagacga agtcaaaaca ggtgtacgcg
     2521 aattaactga aaaatttccg ttgcataaaa aataaaaatt taaattccat tagtaaacag
     2581 aaaagttcaa tgttgggctt ttctgtttat tttttgggaa aacggattct ttgacaatat
     2641 cggtgatgct ctctagtata tgaggtgtaa ttttagtctt cacactaaat ctcagggagg
     2701 aaaagtcatg actgatcgta tctcgttaga aaaggaagca gtagcattca gtgatgcaaa
     2761 tgcgcctcat ccgcgtatct atgaactagc accggaagaa ggaagagttt tactagaaga
     2821 agtacaagaa tcacctgtga acaaatattc cgtcgatatt gaagacttga tggtggatac
     2881 tggcgaatgg ggaaaactca atattcgtct cgtacgacca gaagggaata tggataaatt
     2941 accagttatt ttttatattc atggagcagg ctgggttttt ggcagtgctc aaacccatga
     3001 taaattgatc agagaacttg cggttcgtac gaattccgtc gttgtctttc cagaatatag
     3061 tcgatcacca gaagcaaaat atccaacagc aattgaacaa aattatgctg tgttgcaaca
     3121 gctgaataaa ttagccaagt caaaaaatct ggatctcgag aatctaatcg ttgcaggaga
     3181 ttcagtaggt ggtaacatgg ctacagtcat gacgatctta acaaaagaac gaaagggcct
     3241 cccaatatcc aagcaattgc tgtattatcc agtcacaaac gctgattttg atacagattc
     3301 ctatcatgaa ttcggagaaa actatttttt gacaagagaa ggaatgatgt ggttttggga
     3361 ccaatatacg acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa
     3421 tataaatgaa ctaaatgatc ttccttcagc aatgatttta acgggagaag cggatgtatt
     3481 aagagatgaa ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca
     3541 agtacgcttc caaggaatca tccatgattt tgtcatggta aatgctatgg atcagacaaa
     3601 ggcaacgcgt gcagcaatgg cattatctac ttcttggatc aagaaaaaag aataagcttt
     3661 gcttgtccgc aggaaaactt cttttcaaaa acggattttc ttaggaaaaa tggcgagttt
     3721 gactagtcaa tcacttgatt ttgttttaca attaaaaaga aacgacagaa caaaggagag
     3781 cttaaaaaat gggcaaattt caagtgatcg atcatccatt gattcaacac aaactaacta
     3841 ttattcggga caaaaactgc ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta
     3901 tgttgatggc atatgaagta tcacgggata tgccattgga agacatcgtg atcgaaacac
     3961 cgatcactga aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt attccaatct
     4021 tgcgcgcagg tattggaatg gttgacggta tcttggaatt gattccagct gctaaagtgg
     4081 gacatatcgg tctttatcga gatgaagaaa cattggaacc acatgaatat ttcgtcaaac
     4141 ttccagaaga cattgatgca cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg
     4201 gatcagcaat catggcaatc gatgcattga aagaacgtgg cgcgtcgaac attaaatttg
     4261 tttgtttggt agcagcacct gagggagtaa aagctttgca agaagcgcat cctgatattg
     4321 acatctatac agcttctcta gatgaaaaat tgaatgaaca tggttatatc gttcctggac
     4381 taggggatgc gggtgaccgt ttgtttggta caaaataagt aaatacgaaa aagggtgtga
     4441 cacaactttt gtcacatcct tttttggtca tttttaacgt ttgatgcaga acagcttttt
     4501 cacagctttc gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa
     4561 atctttttta gttctttgta gttaacgatt actacaccct tactttgcgt catcaagttc
     4621 gcattttctg cagtttcatc aatctcaaca acagcggaat ttttcaattg ctttgaaatc
     4681 gttccagttt gcgacttgcc gtgaattgtg aacattacgt gagtattgat cttgaatttt
     4741 tcaccaattt ccgatggtac gatagttgta tcaaattttc caaaagttgc cattttcata
     4801 aaacctgcct ttcacatcaa ttataccacg ttttatgaaa aatatcatga aattaaacta
     4861 attttccatc ggactcttta caatcagcca aatagagtat ataatttctt ttggataagg
     4921 ccagcttagc tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc
     4981 ctatagctgg cattaaaaga gaagacagat atatcaagga tttttaatcc attgatacat
     5041 ctgtcttttt gtttaaagga aataaagtat gctttcttag caatagagca tgtattattt
     5101 ctataaaaat ttaggagaat aaaacgacta gcaatatagc tgtgaaatca aatatcacat
     5161 gaactgccca acttgtaatg atcgagttgc ttttaatggc agcttggttg aataacaatc
     5221 ttgccacccc ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt ccattatcat
     5281 aagtactgta atggataaat ccaaagatga ttgctgacaa gaaactagcg acaggaatgc
     5341 tgaacttact agaaaaaata gcgagaaaat acactgagaa caattcttct ccaagtatca
     5401 tgataggcaa aataataaat aataacgggc tgaaagcctc atttattgga ttgcctgtta
     5461 aaccccattt caaaatttgt tgcaaaaata ccgagactgc ggcactgatt actacattga
     5521 taaaaaaata tttggctata tttttgacag gagcgacagg tttcgagaac atttttttcg
     5581 tatctttttt accaaaaaca agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa
     5641 tgctaataat ggatggcgca aatagcaacc ctaacaaacc gagtacaacg acaatagtgc
     5701 ccaatatccc ttttttaagt tctatttttt ttcctaaaat gttattcatg ataataactc
     5761 ctactattca gtttttgaag atagagtcag tatacatgaa gagttaaaat aggcaatgtt
     5821 tttttatgga tatttgtatt gctaaaaatt tatcttatat attcattttg attatactac
     5881 ttttatttgt aaaaattttc tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt
     5941 ttttagatgt tccgagcgta cttcaatcat ttcaaaggag ttgctactta aatcgatatt
     6001 ttttaaatca gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa acattatttt
     6061 agtgagggaa cagtctacaa aatctgcgct tgataagaca gagtgctcaa aggtactttc
     6121 ttttatgttt gaaccagata aatttacata gtccatttta caattctcga ataacgtgtt
     6181 cccaatataa ctttccatca atttagtacc tgttaattta caattgacaa atttcacgcg
     6241 atgcaacatg ctttttgtca atatacagcc tgaaaaatca cagttttcga atatacagtc
     6301 ggtaatctcc attctttgaa aatcattgga agcaaaaaat gcatacttga aaatagattt
     6361 gtaaataatt ggatggcaga ttatttggtt ttcgatgacc tctttttgtg aaaggatatt
     6421 atacagttca aaatcttcgt tttcatagat atgaaaaaag gatcctttat tcaagcttgc
     6481 ttcattaact ttaggaaaaa tcatatttaa ttacctccat tttcataaag tcatattaac
     6541 aaaagctaaa ccaacttaca ttagcagaaa atgagatagg ttttcgcctt tttcgataac
     6601 gatagtgtcg ctatctcttg aaacgttctt agaattgccc tgagagacac aaattaggcg
     6661 taattctaat aaaaatctta gaataaaaat ttttactgat ttaatttaga atgtttttaa
     6721 aataaaaatc gttgccataa cagtgtattg aaaggtttta acaatccagt aatgatttaa
     6781 aaaaatctag atgtagcgta tactttgttt atagaatgta gtgggaagaa atatattttt
     6841 gggaggaaat cgtgatgtct aaaagtgaag aaatagaaaa catggtacta aaattatatg
     6901 atctcgtttt aaatccagag atcaaagaaa aggaacgtat acttttgata gacgcaaaga
     6961 caggcttaga aaaaggtcag tattatccta aagtcataaa taatttggaa cgtagtttac
     7021 gtccgttagc aatcagggga gaattgtcta aaccagtatc accgttttat atggagatat
     7081 caacaatagg aaaatttgaa aaagaattag gtcgaggaat ggcttcggca ccgattactt
     7141 ttgggcattt ataaaaacga gttattcatc tcaatgaatt aaaaagtata gtcaatctca
     7201 taatgcaggc taggttcatt tatttgaaca gagtctgtat ttttttattc taaataaaga
     7261 aacagatgtt tctttatatt gtattttatc ctttaattaa cacaaaaaca gctagtataa
     7321 actaataatg ttaattaaaa gtgtatttta tgaaactatc gttatttatt aatgttgcat
     7381 ttttgtaagt taaaaattgg aaatatagat tgaaaaaatg aaagcaataa gtaaaataaa
     7441 atgaatatat gcgaaaaaat aatttacat
//
LOCUS       DAXWLF010000129         7421 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXWLF010000129 DAXWLF010000000
VERSION     DAXWLF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.129"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(397..1053)
                     /locus_tag="ACGE4U_002116"
     CDS             complement(397..1053)
                     /locus_tag="ACGE4U_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8394237.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            1246..1524
                     /locus_tag="ACGE4U_002117"
     CDS             1246..1524
                     /locus_tag="ACGE4U_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394238.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1543..2751
                     /locus_tag="ACGE4U_002118"
     CDS             1543..2751
                     /locus_tag="ACGE4U_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8394239.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2899..3264)
                     /locus_tag="ACGE4U_002119"
     CDS             complement(2899..3264)
                     /locus_tag="ACGE4U_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFF8394240.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3411..4184)
                     /locus_tag="ACGE4U_002120"
     CDS             complement(3411..4184)
                     /locus_tag="ACGE4U_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8394241.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4358..5218)
                     /locus_tag="ACGE4U_002121"
     CDS             complement(4358..5218)
                     /locus_tag="ACGE4U_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFF8394242.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(5218..6636)
                     /locus_tag="ACGE4U_002122"
     CDS             complement(5218..6636)
                     /locus_tag="ACGE4U_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFF8394243.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6693..7142)
                     /locus_tag="ACGE4U_002123"
     CDS             complement(6693..7142)
                     /locus_tag="ACGE4U_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8394244.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
BASE COUNT         2154 a         1550 c         1252 g         2465 t
ORIGIN      
        1 cgtgtcacag ccccttttgt tgcaaataaa cggcgttcaa aagcttacat ctacggtgat
       61 aagtccagcc aagttaaaaa cataagagac tgtttttact tggctcttct tattacttga
      121 tgcagaacag ctttttcaca acctcttggt aaacggtgtt ctcctaccaa ctcctcggtc
      181 acaagtcaca atattcatca aacatgaaaa gctgtttttt gaaattgttc cttgtgactc
      241 agagttaaac ggcaggctca caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg
      301 tattagctca aaaaacggca aaatatgagg aactattttt ccgtttgttt ctctaatact
      361 caggtcaaaa cgctgatttc acaacctctt tgttcatcaa aagataatat tgtcttctaa
      421 caagtccata ttcaaaataa cgatatgatg gtctttaacg gtgataatac catctttttt
      481 cagttgtttt aaaattctgt ttacactgct agcagttgag atgccacaaa atttggcgat
      541 ttcttcattc gtgatcgaaa aatcgatcag catcccttct tctgtccgca caccgaataa
      601 atcatataat tcgtaaagtt gcgtacaaac agctcccagc tttccgttta atagcatttg
      661 ctgcattttt ttcatcgaat aaagcagacg atagcggtaa tactctttta catagtgttg
      721 tagctttggg tcttggttga tattttgcca aaagcgcaca cgattgattc gataaaactc
      781 tgcatattct gattcaactc gaatattata cggcgaatcg atgaattgtg aatattcgtc
      841 ttttaaaaga gagacgattt caagatcttt gatatagcga aggttaaatt ctctcccatc
      901 atttgaaata acacttgctt ttaccgttcc ctctttcaag atatacgtat gggaatcttc
      961 catcccttcg aatgtcaaat atttatgata tcctttgcgt ataatcggaa aatcattttt
     1021 atccaaatat tcctttaaga ctctactatc caatgatgct cccccatcca aactttcatc
     1081 taaaaagatc atacagtact ttaaaacttt ttaaagtcca aaggtatttt actaatgata
     1141 ttaacatttg ttaagtacct ttttccatca cttatgcgat ttttttcctt ttttttattc
     1201 gttatactgc agaagtaaaa ttttttaaga atagagggaa aaaacatgaa ttactggatg
     1261 aacacaatca ttaatcgatt agaaacagca tatcaaacaa gatttgatat gaaagcttct
     1321 ttagtctttt taaatgatgc gtatcaaaac tcgatcgaat tgatcaaagc agtggatgag
     1381 aatccaacaa acgaatgcga agaattttta aatctgttta tgtctacaag agatttattt
     1441 attcgccaac ttgttgatcg ctatccatcc aattatcacg atgtagaagt tcaaatacaa
     1501 aaattaaaag catattcagc ttaaggggaa tgtataacat acatggaaaa gaaattattt
     1561 aataaatatt ttattggaat cacattgatc aattttattg tctacctgat ttattatctg
     1621 ttgatggtca tcatggcagt cattgcgcaa gacaccttac acgccagctt gagccaagca
     1681 ggttttgctt ctgggatcta tatcatcgga acattgctgg cacgcttatt catggggaaa
     1741 cagttagaac tatttggtcg gaagtttacc ttaagaggcg gcgcgatctt ctatctcctt
     1801 tcaacattag catatcttta tacgccaact atcggggcat tgtatttagt tcgattcttg
     1861 aatggattcg gctacggtac agtttctaca gctacaaatg ctatcgttac tgcatatatc
     1921 cctgcatcta aaaaagggga aggcatcaat tattatggat taagtactag tttagctgcc
     1981 gctgttgggc catttttagg aatgattttg ctgaatatga ctgattttcg tttcattatc
     2041 tggttttcaa ttgtactggt cttctttgtt acgattgcct gccttgtctt tcctgtaaaa
     2101 aatattccat tatctgaaga acaaaaaata tctcttcgct catggaatat caatagtttt
     2161 attgaaaaga aagcactttt cattacagga attgcctttt tgatgggtct ttcttattca
     2221 agtgttttat catttttatc ttcttataca aaagtaatcc acttagttgc tgctgggtca
     2281 tttttcttcg tcgtatatgc tttggtcatc acatttacac gtccattgac tggaaggatt
     2341 ttcgatgtca aaggagaaca atacgtgatg tatcctagct atttattctt aacagcagga
     2401 ttatttttac tgagtgtaac gacgaatagt gtgacattgc ttatctctgg agcattagtc
     2461 ggcctaggtt atggtacgtt tatgtcaaat ggacaggctg tttgtttaaa aatcgtccgc
     2521 gaacatcgaa tcagtattgc tttatctacc tactttattg gtttagacct aggcttaggc
     2581 gttggtcctt atttgatggg gatgctaaaa tctcttactt catttagagg attgtatgtg
     2641 attgccggag tcattccatt gatatgtaca gcactctatc ttgttaatgg ccgaaaacat
     2701 agtcatgaag cagagggcga attgattgaa tcattgaaaa atgaaatttg atcttgatga
     2761 atagataaaa aaaaccgttc agatctgaaa ataatctgag cggttttttg agtaaaaata
     2821 gtttatgatt attcttaatt aaacatctat ttatttttaa agctttcatt tatcgataag
     2881 aatagtttaa ctgttgattt attccatttt ccgttcatct ggtacgatgc cttgacttag
     2941 caaccacttg tggaagaacc attctgccac accgatcaat agagcaggaa tcaatacttt
     3001 catccaaata tcgactgtat cattcataaa atatagatag agccacaaga taactgcagc
     3061 tgaaccagca tctgcgatcg tcgctgcgat gttccctact ttacgtaaga tcaacagatc
     3121 ccctagcaca tagatcatga ctacaataat taccccaaca atcgttgaat ccataaagct
     3181 aacaccgtat aaacctgtca gcacgaccca cagaatcgcc cagatcatga ttgccttcac
     3241 taccaacgcc ttcacatgtt tcattttctg cactcctttc tcctggataa aaaagtaaga
     3301 ttatctaaca agataacctt ttcattatta taccatcggc tgagagaagc gagcaaagat
     3361 aaggtcctat ctaaaaatga ggaagccact tagaacattc acgtaataat ttatccgtac
     3421 agacattcga tatggtgttc ctttactttc gcatactgtt cttttgtcag ttctttactt
     3481 gtcaccagat aatcgatttc atgaaaagca atggcttgat gcatgaattt atgtgcaatt
     3541 ttttgctgtg taactaaaac ggcaagccgg tctgattgtt ttctaaaagc ttttttgacc
     3601 attgcctctc cttcatgagt ttctgttact ccttggctga aatctagccc gcgacaactc
     3661 attatcgcta aatccgcata atatgacaat gcatcgttat aggcttttga accgttgatt
     3721 gttgctcgtt tcggaacaat tgcccctccc aatacagata cttttgccgt tgtatattca
     3781 ctcaacatag cagctgtgac gatgccattc gtaatgatct gcaaaccatc aaatcttgct
     3841 aggtttctga ctaagtgtaa agaggttgta ctggaatcca agaataagac catatcatct
     3901 cgaatcaaag aagaagctgt atctgcaatc ttacgttttt ctgtttcttc ttctaatttg
     3961 gtgatcttca tcaggtaagg cacgtcaatt tttcgattgt caataagcat ggcgccaccg
     4021 cgaactctac ggatcaatcc acttttttcc agaaaaacaa ggtcacgttt aatcgtggct
     4081 tcgctataat ttactaactc gatcaaatcg gtgactcttg caaattggtt gttttttaga
     4141 taattcacaa tctcctgctg tctattctcc acttcattca acataaaatc accccattta
     4201 ctttatatag taacaggaaa aaagaggttg tgacagaagt gattggcttc gagaaataag
     4261 aagaaatttt tcgaaaaaac tacttctgaa catcgtgtgt ggttaagagg gtatgacaag
     4321 actattgtca taccctcttg accacacatt tttattttta gtataaacta gccttgccta
     4381 ctgtgttcaa aatttccatt ttatgtttga ccacttcttg taccttttta ttagctgctg
     4441 gataaattgc attcggctcg tacatcgatg gattttcact caatatttct ctcaaagcat
     4501 tgaaaaagac gcttttaaga tctgagctca aattcacttt ggcaactccg tattttactg
     4561 cttcgctgac ttctgcgtcc ggattacctg aaccgccatg taaaacaaat ggaagatcga
     4621 tttgttggtt caattctttc agtaagtcga tatttaattt tggagttctg tttttcggat
     4681 acaagccatg cgctgttcca attgctacag caagtgtatc gattcctgtt tctttcacaa
     4741 aaatcttcgc ttgatcagga tctgtataaa tgattgtttc agcacctcct tcagcagaac
     4801 cattattccc aatcgttcca agctcagcct ccactgagac atttaccgca tgagctaacg
     4861 caaccacttt tttcgtaatc tgtacattgt cgtcgaatga tttcaaagac gcatcgatca
     4921 tcacggaaga atagccgttt cgaatcgccc gtaagacatc ttctatcgta ccgccatgat
     4981 ccaagtggat aacgatcggc agtttacttg tataagcata ggctcttact gccgcaacaa
     5041 actcatcact taaataggcg atttcatcag gatgaatttc taagattact ggtgcttcta
     5101 atctctctgc ttcttcgatg attgctttca tcatatcata gctgcaaata ttgaaggctg
     5161 gtactgcaaa ttgattctct ttagccactt tcaataactc tttcattgta tataacatta
     5221 cgcttccacc tcatttgtta gattttcatt tttcgtccac ttgtcttgtt ttttctcttt
     5281 cactgttttt ttcaatagtc caagcataaa ggctgtagtg actgtacctg atgcaattgc
     5341 caagacatac ataggtaaat tgttcataac atttggaatt agcatcaccc aaatccctcc
     5401 atgaggtgcc attgaagtaa ctcctactgc catggaaatc cctgcagcga ctgctgaacc
     5461 tgtcatcaaa ctaggtatca cgcggatcgg gtcagatacc gcaaacggta tagcaccttc
     5521 tgtaatatac gaagcgccta aaatccagca tgattgacct gaagtccgtt cttctttggt
     5581 aaatttatgt ttgaataata aagtggctaa agcaatacct aggggaggtg tcattcctgc
     5641 agccatacaa gcagcaatcg gtgcatttac tccagctgac atcaaaccga tcgagaaagt
     5701 cgaagtcgat ttattgatcg gcccacccat atcagctgcc atcatgatcc cgataagtaa
     5761 tccgaacaat agcccggatg tatttcccaa gctattcagc caatttgtca aagcatccaa
     5821 cacccatgtg atcggttgtt caatcacaaa taccatcaaa agcccaataa ttaagatcga
     5881 taaaaagggg acgacaatca gctgataaat agcagaaaat tcttttttta cgctaatttt
     5941 atttgccaga aaatcacaga tataccctgc tagcacgcca ccaatcattc cgccaagaaa
     6001 acctgaacca cctgtagagg ccagtaatcc ggcaacaatt cctggggcaa aacctgcttt
     6061 tccagcaatc gatgtagcaa tccccgcgcc taacgcagga accatcatgg caaacgctgt
     6121 atctcctccg atccttgaaa atgcagctgc taacacatta tagtctgctg agttgggatc
     6181 agccgctgta atcccaaagg caaaactcag tgcgatgatg attccgcctg cgatcacaaa
     6241 tggaagcatg taattcacac cattcatgaa atgattatag attgtcggct ttttctcatt
     6301 tgcagctggt tctttttcat tagacacaac gaatacgcca atatctctca atgcttcgtc
     6361 tattactact tgcggttctt tgattgctcg gcttgtagag acacgcttta cttttttccc
     6421 ggcaaatcgg tccaattcga tttctttatc tactgcaaga atgattgcat ctgcctcttt
     6481 gatttcttgt tcagataact gattttctgt ttttacacct tgtgtttcga attttgcttg
     6541 atggtttaga tttcttgctg ttacttctaa tttttctgcc gacatataag tatgagcaat
     6601 tccagtcggg caagcagtta caccaacgat tttcatgatt attttcctcc tttttcttca
     6661 ctatcgatac tcggacaaag actctactat tttcattctt gactaatctg tctgatgata
     6721 tctgctggcg tagtggcttc ttttattccc tttctaaaat ctgcattcat caatcgtctg
     6781 gagagtgtgc tgagaatctc tagatgcata tctgctgatt tttcaggaac tacgatggca
     6841 aaaatatatt caattggcat ttcatctatc ccgctatagt ccgttaattt ggctgtatga
     6901 aaaaaacaca gtgctggttt tgtcacatat ttgctttttg catggggaat cccaaatcca
     6961 tcttctattc ctgtggagat ctcctcctct cgtttgaata gtgccttaac gactttttga
     7021 acatttctta ccttatttgt ttttttcaac acatccccta ttcgctcaaa taatgatcct
     7081 tttgaatcta tcgatggatc gaaaacaatc agttcttctg cgatcaattg atcaaacctc
     7141 ataaaaaacc tctcccccac aaaaaatgtt aacgttttct tggaactgag gtcagtatag
     7201 catcgctagc tgtaccgttc aatattatga atgaacagag gaaaaagacc catcatccgc
     7261 gtgtataagt cattaaaatg aacgatctag ctcacaaaca atcgcaagtc tataacaaaa
     7321 caaaaagcct tccttgaaag gaaagcttag cttctaattt taggtaaatg gttttttact
     7381 tcgaaataac ccctcttcat ttcgttaata taaggtgttt t
//
LOCUS       DAXWLF010000130         7267 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXWLF010000130 DAXWLF010000000
VERSION     DAXWLF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.132"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            113..760
                     /locus_tag="ACGE4U_002124"
     CDS             113..760
                     /locus_tag="ACGE4U_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFF8394245.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    889..1105
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1153..3798
                     /locus_tag="ACGE4U_002125"
     CDS             1153..3798
                     /locus_tag="ACGE4U_002125"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFF8394246.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4108..5421
                     /locus_tag="ACGE4U_002126"
     CDS             4108..5421
                     /locus_tag="ACGE4U_002126"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFF8394247.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5411..6064
                     /locus_tag="ACGE4U_002127"
     CDS             5411..6064
                     /locus_tag="ACGE4U_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8394248.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6119..6811
                     /gene="radC"
                     /locus_tag="ACGE4U_002128"
     CDS             6119..6811
                     /gene="radC"
                     /locus_tag="ACGE4U_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFF8394249.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6961..7161
                     /locus_tag="ACGE4U_002129"
     CDS             6961..7161
                     /locus_tag="ACGE4U_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8394250.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2399 a         1232 c         1595 g         2041 t
ORIGIN      
        1 ttgaacaccg ttttatttgt ctcacaccct tttccttcct acttgtaacc ttgctcccgt
       61 gttagaaaca tgttacacta aatatgtgaa attattaaca aggagcgtcg ttatgcgaga
      121 aaaaacagat acactgccaa aagcaactgc gaaaagatta cctttatatt tacgctactt
      181 gaagatgctg gatgactcag gtataagtag aatcaaatca aatgagttca gcgaaatcac
      241 ccaaattcct tctgcaacga tccgcagaga cttctcgcaa ttaggcgagc taggcagaag
      301 cggatatggc tatgatgtcc cttttcttat cgatgtattc aataatatcc ttaatactaa
      361 agaagaaaaa cgaatcgcat tagtaggata tggaaatctt ggcaaagcat tgaaacacaa
      421 caattttaga agaaatgaaa atctaaatat cgtgtgcgtc tttgataatg atccagcact
      481 gatcaatcga gtaattgatg gagaaatgat ctatccgatt gatcgtttcg cggagatagc
      541 caaagcaaaa aatgtgacag tagcaatctc tacggtacca agtaaatatt cacaatctgc
      601 tattgatgaa attgtcaaag gaaatgtaac cgctatactt aattttgcgc ctgacagagt
      661 aactgtccca gcttatgtca atgttcaata cattgatttg acaaccgagt tgcagacgct
      721 tatttacttt gatgagaatt actcagaagt tttttcttag gaaaatttga gtaggcttat
      781 tgatcttgcg agaactcgtc gtaattcttt caaaaatttt ttagtactaa atgaagtggt
      841 tttttcacga ccacttgttg actaaaaaaa aaaaccattg tagaatgaag cataacgctg
      901 aacaagagga gtaactggca cattcattct tagagagaga actatctgct gaaaggttct
      961 caatgacaac cagtgaaggt agcttgggag tttctgtctt gaatggagta gagatagacg
     1021 agtcgtgatc gttaactcac ttgagaggca ataatgattg attattgcaa tttaggtggt
     1081 accgcgtaaa tcatacgtcc tagaatacga aagtatttta ggacgttttt tagtttaaag
     1141 gaggaaaaca gcatgacgga agaaaataac ctatcaacaa aatataatcc gcaagaagtt
     1201 gaagctggac gttatcagca ttggttagaa caagatttgt ttaaaccgag cggagataaa
     1261 aaagcaaaac cttattcaat cgtaatcccg cccccaaatg ttactggaaa acttcattta
     1321 gggcatgctt gggatacgac cttgcaagat atgctgattc gtcaaaagag aatgcaagga
     1381 tatgatactt tatggctgcc tggtatggac catgcaggaa tcgcgacaca agcaaaagta
     1441 gaagcgaagc ttagagagca agggatttct cgttatgatc taggtcgtga aaagttcatc
     1501 gatcaagtgt gggaatggaa agaagagtat gcgagccaca tccgcgaaca gtgggctaaa
     1561 atgggcttat ctttagatta cagtagggaa cgttttacat tagatgatgg attatctgaa
     1621 gcagtacgta aagtttttgt taccttatac gaaaaaggtt tgatttatcg tggtgaatat
     1681 atcatcaact gggatcctca agcacgaaca gctctatctg atatcgaagt tattcataaa
     1741 gatatcgaag gcgcctttta tcatatgagc tatgaactag cagatggatc aggcgtagta
     1801 gaaattgcga caacccgtcc agaaacgatg ttgggagata cggcgattgc tgttcatcca
     1861 gaagacgagc gttatcaagc gctcatcggc aaaaaagtga ttctaccttt agtcaataaa
     1921 caaatcccaa tcattgcaga tgaatatgta gatatggaat tcgggacagg tgtagtaaaa
     1981 atcactccag cacatgatcc aaacgacttt gaggtaggta atcgtcatga tcttccacgc
     2041 gtaaatgtta tggatgatga tggtacgatg aatgaactag caggaaaata tgctggaatg
     2101 gaacgttttg ctgctcgtaa agcaatcgtg caagatctga aagaaatcgg tcgattgatt
     2161 aaaatcgata cgatgatcca cagtgtgggt cattccgagc ggacaggtgt cgttgttgaa
     2221 cctcggctgt ctactcaatg gtttgtaaaa atggcgccgt tagcgaaaaa agccattgat
     2281 aaccaagaaa cagatcaagc agtggaattc ttccctccaa gattcaatca aacgttttta
     2341 cgttggatgg aaaatgtcca tgattgggta atctcacgac aattatggtg gggacatcaa
     2401 attcctgcat ggtatcacaa agaaacaggt gaaatgtatg tagggatgga agcaccagcg
     2461 gatagtgaaa actgggtaca agatcctgat gttttagaca cttggtttag ttcagctttg
     2521 tggcctttct caacaatggg ctggccagat acggaaagcg aagattataa gcgatacttc
     2581 cctactagca ctttagtaac cggttatgac atcattttct tctgggtaag cagaatgatt
     2641 ttccaaagct tggaatttac tggcgaacgt ccatttgaga atgtcttgat tcatggattg
     2701 attcgtgacg aacaagggcg gaagatgagt aaatcattag gtaatggaat cgatccgatg
     2761 gaagtaatcg aaaagtatgg agcagatgct ctccgctggt tcttatcgaa tggttctgca
     2821 ccaggacaag atgtccgctt cagctatgaa aaaatggatg cttcttggaa ctttatcaac
     2881 aaaatctgga atgcttcacg tttcgttatc atgaatatcg aaggaatgac cgtagaagag
     2941 atcgatttat ctggagaaaa atcagtggct gaccggtgga tccttactcg tttgaatgaa
     3001 acgatcgaac gagtaactga gctatttgat cgttttgaat ttggtgaagc aggtcgacaa
     3061 ctgtataact ttatctggga tgatttttgt gactggtaca tcgaaatgag taaagaagta
     3121 ctttatggag atgatgctgt ttcaaaacaa accacccgta gcatcttagt ccatacactt
     3181 gatcagatcc tacgtttatt gcatccaatc atgccattcg ttacagaaga aatatgggaa
     3241 catattcctc atcagggaaa ttctctagta gtggcagaat atcctgttgt acgtccagag
     3301 tttgatgatg aaacagcttc taaagggatg gaagtattga aagaattgat tcgagccgtt
     3361 cgaaatattc gttcagaagt gaatacacca ttgtctaaac caattacgtt gctaattaaa
     3421 acaaacgatg ctaaaatcga tcaattttta gttgaaaata caagctatat cgaacgcttc
     3481 tgtaatcccg aagagttgac gatatcaagt gagatcactg cgccagatct agcgatgtca
     3541 gctgttctga caggtgcaga aatcttcttg ccgttagcag gattgatcaa tattgaagaa
     3601 gaaatcaaac gattagaaaa agaactggaa aagtggacaa gcgaagtaaa acgtgtccaa
     3661 ggaaaacttt ctaatgaacg ttttgtttct aacgcgccgg aagaagtagt agaggcagag
     3721 cgtgcaaaag aaaaagatta tttagagaag caaacggctg tcaaagaacg aatcgaaagt
     3781 ttgcgtacta ttcaataaga atagaaaaag agagtgtgaa atcagctagc tattactaaa
     3841 agctagctga ttgaacatcg ttacaggaaa gagtttgagc ctcactctta gcttcgattc
     3901 ataaggagat gtgacataaa ttttgtcgca tctctttttt agtctactct tattatgtag
     3961 gagaaggagg gaggtggatc atgtatcaag agtcaataaa gtagttgata aaatcgttcc
     4021 tctctctgtt tcttttctct ttgtcatact tttatttttc ttatgtgttc tatttgttat
     4081 aataaaagag aaatggggga attgttgatg acgattgaag aagcaataga atggatccat
     4141 agtcgattac catttggttc acgtcctggc cttgaccgag tcgaagcttt attagaagtg
     4201 gtagggcatc cagagagaaa agtaaaaacg atccatatcg ctggaacaaa cggaaaaggt
     4261 tctactgtag cttatttgag aagcctatta gaagaggctg ggctgacagt aggtacattt
     4321 acttcacctt atatcgaaac gttcaatgaa cggatcgcaa tcaatggcaa aagcattcca
     4381 gacaatgatt tgatccagct tgtaaaaaaa tatcagccac tgatcaaaca acttgatcaa
     4441 tttgaagcag tagctggtat tactgaattc gagacattaa cagcaatggc atttgattat
     4501 tttttgatga agaaagtgga cgtagcgatt atcgaagttg gcttaggcgg cttattggat
     4561 tctaccaatg tagctgtacc aatgctgacg ggaataacaa cgattggact tgatcatacc
     4621 gaaatacttg gagatacaat tgaagaaatc gctgcacaaa aagctggaat catcaaacaa
     4681 ggcgttccag tagtaacagg aaatattgcc ccagaagctt ttactgtcat ccaacaaaca
     4741 gctaaagcaa aaaatgcacc gctttatgta tacggggaaa attataaaat aaactatctg
     4801 catcctgatg agatgtgggg agaagtattt gaatttttta atgaaatagg aaagatccct
     4861 caattaacca ctccgctttt agggcaacat caagctgaaa atgcaggagt agccatcgaa
     4921 ttgtattttg tttactgtca attggaaaag ctgcctttcc gagaaaaaga tgttttgaag
     4981 ggattgaaga aatctcgatg gccaggaaga atggagcgtt tgagtaacga gccattagtt
     5041 gttttagatg gtgcccataa tacgcatgca atgaaccggc ttgttgagaa tttgaaaaaa
     5101 gaattcaaag gatatcaaat caacattttg ttttccgcct tacgaactaa aaatgttaga
     5161 gaaatgctag aacgattgca gcaagtacca aatgtccata tttatttgac gacctttgaa
     5221 catccaaaag ccatcgaatt aagtgaagta tcagatttag caagtgagag agtatcaatc
     5281 gtttcattgt ggcaattcgg attagcagat atattggaaa agatgacaaa tgaagacgta
     5341 ttagttgtga ccggttcact ttatttcatt tcagaagttc gccatttatt aatacaatta
     5401 ggaggagatc atgaagtata atgcagtgat ttttgacatg gatggtttgc tgttcgacac
     5461 agagttagtg tattacgaag ccacacaagt agtagcagac aagatgggtt ttccttatga
     5521 taaagacctg tatttaaaat tcttaggtgt ttcagatgaa gaagtatggg cgaattatca
     5581 tatgattttc tcggaatttg gcaaaacaag tgtacaaaga ttcatagatg attcatacca
     5641 ggaaacgctt gaacgttttt cgcaaggagc ggtacaaatg aagcccggtg tgttggaact
     5701 cctctctttt ttagaagaac atcagattcc caaagttgtc gcttccagta accaacgagc
     5761 agtcattgaa cttttattga agaagaacaa tctgtttgaa cgatttgaga cgattgtttc
     5821 tgcagaaaat gttcaacgag caaaaccaga cccagaaatt ttcttgttag ctaatcagaa
     5881 gctaggtaca gcgaagaaag aaacattgat tttagaagat tcacaaaatg gtattttagc
     5941 agcttctgcg gctgagatcc cggtagtcat ggtaccagat ctgcttccac catcccaaga
     6001 attgatcaaa aaaacagctg gggttgtttc ttctttacat gaaatccctg cattgttaaa
     6061 ataaaaatcc atctcttttc gtatcttatt ttagataaga tagaaaggag atgttttttt
     6121 gggaaaacga ttgatcaaag aagtacctac tagctcttta ccaagggaac gtatggaaat
     6181 ttatggcgcg gaagctttat cggatcagga gcttttggca atcttactgc gtaccggtca
     6241 acacccttat agtgtgatgt cgattgctgg aaatctgtta aaaacgtttg gaggactggc
     6301 ctctttacga caggctactt tgcatgagtt agaagagatt cgagggatag gacgcgtgaa
     6361 agcaatcgaa atcaaagcct tgatcgaatt aggtcgacga attcagacgg atcatgaaag
     6421 agcggctcca ccggttcgta ccagctacga attggcccag caattgattc tggaaatgaa
     6481 agatcacaaa caagaacatt tggtttgtat ttatttggac acgaagaatc aagtgatctt
     6541 aaaaaaaaca gtgttcatcg gttcgctgaa tcagtcagtc gctcatccta gagaaatttt
     6601 ccattatgct gtacgttatt gtgcagcaag gatcgtttta gcgcataatc atccttctgg
     6661 taatgttatt ccatcgcagc aggatatgaa ttttaccaaa aggatacaaa agtgtggaga
     6721 aatgatgggg atcactgtac tggaccactt gatcatcgga cgcaaacgat attttagttt
     6781 gagagaagaa ggaatgatgg aagagaagta agttgctttc ttgcaaaaaa aatgaagcaa
     6841 gtgtacaatt atattgtaat tttgtttggg aatagaagtt gaaacatctg ttgcacctct
     6901 atcgtacacg agcaatgtta caatttaaat tagtgctttt ggcacgagga ggatattatt
     6961 atggaaaacg gaacagtaaa atggttcaac gctgaaaaag gatttggatt tatctcacgt
     7021 gaagatggaa gcgacgtatt cgttcacttc tcagctatcc aaggtgacgg attcaaaaca
     7081 ttagaagaag gtcaagcagt gactttcgac gttgaagaat cagaccgcgg cccacaagct
     7141 gcaaacgttg taaaagctta attttatctg taaatcaaat tgatggtaga catcaatttt
     7201 tacgagagaa caaagcgagt aacaaaaata cctgccacaa gagacaattt ctcttgtggc
     7261 aggtatt
//
LOCUS       DAXWLF010000131         7250 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXWLF010000131 DAXWLF010000000
VERSION     DAXWLF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.133"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(43..1449)
                     /gene="lpdA"
                     /locus_tag="ACGE4U_002130"
     CDS             complement(43..1449)
                     /gene="lpdA"
                     /locus_tag="ACGE4U_002130"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFF8394251.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            complement(1457..3100)
                     /locus_tag="ACGE4U_002131"
     CDS             complement(1457..3100)
                     /locus_tag="ACGE4U_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFF8394252.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEIQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            complement(3125..4102)
                     /locus_tag="ACGE4U_002132"
     CDS             complement(3125..4102)
                     /locus_tag="ACGE4U_002132"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFF8394253.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(4105..5214)
                     /gene="pdhA"
                     /locus_tag="ACGE4U_002133"
     CDS             complement(4105..5214)
                     /gene="pdhA"
                     /locus_tag="ACGE4U_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFF8394254.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            5376..5633
                     /locus_tag="ACGE4U_002134"
     CDS             5376..5633
                     /locus_tag="ACGE4U_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394255.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            complement(5675..7099)
                     /locus_tag="ACGE4U_002135"
     CDS             complement(5675..7099)
                     /locus_tag="ACGE4U_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFF8394256.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         1872 a         1624 c         1305 g         2449 t
ORIGIN      
        1 cgcaactgat ttttcagttg cgtttcgcct gtattattca aatcaaatat ggattggtaa
       61 acctaatgct aattccgaag catccatgac gatttcgcca agagatggat gaggatgaat
      121 cgtcaaggcg atatcttcag cattcattcc tgattcgatt gctaaagcta attcagagac
      181 catatcactt gcgccgactc caccgatttg cgcgccaata agcacgttat cttcgatcgt
      241 cgtaactaga cggatgaatc cttctgtttt accaagcgat aatgcccgac cgttccctga
      301 aaatgggaat ttatatgctt ttgcttcgat accagcatct tttgcttctt tgatcgtcat
      361 acctacagaa gccaattcag gatcagtgaa ggctactgcc ggcattgctt tataatctac
      421 agctactttt ttgccagaga ttgcttcagc agcgattttt gcttcataac tggctttgtg
      481 tgctaatgcc gcccctggaa cgatatcacc gatggcatag atgttaggta cgttcgtacg
      541 tccttgttta tccaccgtga tcaatcctcg ttctcctact tcgacacccg cttgttctaa
      601 gcccatatca tctgtattcg gacgacgacc tacagtgacc atcacataat cagcagtaac
      661 ggattcttct ttcccgtcca ctgcatattt cactgtcacg ctgtcaccgt tatcgactgc
      721 ttctttcgcc attgcattcg ttacgactgt tacgcctttt ttcttgaaat catcttctac
      781 taattttact agatcttttt cgtaagttgg taaaatttgt ggtgagcctt caagaatcgt
      841 tacttctgct cctaagtttg cgtacgctcc gcccaattcg gcaccaatca caccgccacc
      901 gatgatcaca aatttcttag gtacttcttt caatgctaat ccgccagtag aatctaacac
      961 acgtcctccg aatttaaatc ctggaatctc gattggacgg gaacctgttg cgatgattgc
     1021 gttattgaac gaataagttt gagctgaatc tggatgaatg acgcgtagtg tgtgatcatc
     1081 tacgaagaac gcttctcctt cgattgtttc aaccttatgt ttttttaata ggaaaccaac
     1141 accagaagtc aacgtatgga caactttgtt ttctttccat tcttgtgttt ttgcaaaatc
     1201 tagtttgacg ttttcactag ttactccaaa catttcagaa tctagtgact cttgataatg
     1261 gtgtcctgca gctatcagtg cttttgaagg aatacagccg acattcaaac aaacaccgcc
     1321 aatatactct ctttcaatga ttgctacttt ttgtcccatt tcagcagcgc ggatggcagc
     1381 tacatagcca ccaggtcccg caccaattac aactgtatct aattctacag caaaatctcc
     1441 aactaccatt tgtttatcat ccttccatca ttaatagctc tggatcagct aaaagacgtt
     1501 tgatattatt cattgcttgt tgcgcagttg ctccatctac gatacggtga tcgaaactta
     1561 atgacagttt catcacacga ccgacaacga tttcgccttc agcatttacg attggttgtt
     1621 gtgcaatcgt tcctacacct aaaatagcta cttctggata gttgatgact ggtgtaaacc
     1681 aaccgccacc tacagatccg atattgctga tcgtaattgt tccattgcgc atatcttctg
     1741 cagatagttt gccgtcatga gctaattttg ctttctcatt gatttcgtct gcaatagcaa
     1801 acattccttt gcggtctgca tctttcacat ttggtacata tagtccgtga tccgtatcag
     1861 ttgcaatacc gatattgtag taatgtttat aaacgatttc ttggtttgca tcgtcgatag
     1921 atgcattcaa tactgggtat ttcttaacag tcgctgttaa agctttcacg acatatggca
     1981 agaaagtaag tttcgtacca tttgcagcag caacttcttt aaatcgtttc cgattatccc
     2041 atagtttaga cacttctact tcatcatgca atgtcacgtg tggagctgta tgcttactgt
     2101 tgaccattgc tttagcgata gctttacgtg ttggcgtcat tgctacacgt tcttccaagt
     2161 ctcctaagtt ggacttgaat ggttttgctg gtgcagctgg tttgctttct gctgcaggag
     2221 ctgcttcttt cggtgctgcc gcttctggcg cttctgattt cgctggtgca ctgcttggac
     2281 cacctgccaa gaagttttcg atgtcctctt ttgttactct gccacctttt ccagttgcag
     2341 ttacttgtga gatatctaca tctttttcac gggcaaattg acgaacagat ggcatagcca
     2401 atacacgttt atttggatcg gcagcttcaa cgacacttgc tgaacctgat gtttccactt
     2461 tttcttttgg tgcttcagct gatggcgcac tagttgacgg tgcactattg tgtccaggag
     2521 catcgatttc tactaataca tccccaacat ttgctacagt gccttctgga acaatcacgt
     2581 ttttaactgt ccctgtgaca ggtgacggaa tttcttctac tgatttgtca ttttgaactt
     2641 ctagtaatgt atcatcttca ttgatcgtat ctcctggttt gacgaaccat ttgacgattt
     2701 caccttctgc gattccttca ccgatatccg gtaacttaaa ttggaagact ccttcgcttg
     2761 acgatccagc tgattctgta tcaacagtgg gttcagcagc cggtttagct ggagtttggg
     2821 attctgcggc tacaccactg tccccttcat tgtcttcgtg tcctggtgca tcgatttcta
     2881 ctaatacatc cccaacattt gctacggtac cttctggaac aatcacattt ttaaccgttc
     2941 ctgtaacagg tgacgggatt tcttctactg atttgtcatt ttgaatttct agtaatgtat
     3001 catcttcatt gatcgtatct cctggtttga cgaaccattt gacgatttca ccttctgcga
     3061 ttccttcacc gatatccggc aatttaaatt gataagccat gactttgttt cgtcccttct
     3121 tttcttaaaa ttccgcgatt tcttttactt tgtcttcgat atcttttgca tttggcaacc
     3181 agacattttc tgcttgtcca aatgggaaga ctgtatcagg tgcagaaact cgtccgattg
     3241 gggcttctaa tgaaaggata gcgcgttctg agatctcaga aactacttgc gcggcaacgc
     3301 ccgcttgtcg ttgtgcttct tggacaacaa ctacgcgacc agttttttcc acggattgaa
     3361 tgatcgtttc tacatctaat ggcgcaactg tacgaagatc aatgatttca acagatatat
     3421 tttcttttgc aagattgtct gctgctttga ttgcttcacg caccattgct ccgtatgtga
     3481 taatagatac atctgttccc tcacgagtaa ctgctgcttt atccaaagga acttcgtaag
     3541 cttcatccgg cacttcttct cggaatgagc gatatagttt catgtgttct aggaaaacaa
     3601 ccggatcgtt acttctaata gaagaaatca atagtccttt tgcatcataa gggttggaag
     3661 gaatcactac tcgaatacca ggagactgag cgatcaaacc ttctaaatta tccgaatgca
     3721 attctggtgt atgaacaccg ccaccaaatg gcgaacgaat tgtgatgggt agatttctag
     3781 tgccgctcat tcgatatctt gtacgagcca tttgagctac tacttcgtcc attgcttcga
     3841 aaataaaacc aaagaattgg atttcaggaa ctggacgaaa tccttctaac gcaagaccga
     3901 aactcaatcc tgcgattcca gattcagcta aaggagtatc gaatactcgg tcttctccga
     3961 acttttcctg caatccttca gttgcccgga aaactcctcc gtttttccca acatcttcac
     4021 caaaaacaac gacattctcg tcattttcta gttcaagtgc taaggcatct gtgattgctt
     4081 ggatcattgt tttttgtgcc atgattattt cgactccttc gcttcataaa ttgcaatttg
     4141 ttctttgatt gattgtggag atacttcgaa catgtttttc aagaaatctg aaaccttttg
     4201 ttttggtact ttgtcggctt cagcaatagc tgctttgatt tcttctttag ttgcttcgat
     4261 gacttgttct tctttttctt cagaccaaag tcctttttcc gtcaaatatt tacggaagcg
     4321 gatcaatgga tcttttttct gccaatcgtc atctgtgtct tttgtacggt agcgggttgg
     4381 atcgtcacca gatagtgtat gtggtccata acgataagtc agtgtttcga ttaggactgg
     4441 tccatttcca ctgatcgccc attcccgtgc cattttagat acagtatata ctgcaagcgg
     4501 atccattcca tcgacttgaa taccaggaat cccagctgcg acagcttttt gtgcaagtgt
     4561 ttttgctgca gtttgttttt cacgaggtgt agagatcgca tacccgttgt tttggatata
     4621 aaatacggca tttgcatggt aagcgccagc gaaattgatt gcttcgtaaa aatcaccttg
     4681 tgatgaaccg ccatcaccag tataagtgaa gacgacattt ttcttgtttc gtttcttcaa
     4741 acctaatgca acaccggctg cttgtacata ttgtgcaccg atgataattt gtggtggtag
     4801 agctttcaat tcttccggat aagaattacc tgcagcatgt cctcgagacc ataaaaaagc
     4861 ttgagataaa ggtaaaccat gttttactag ttgaggtaca tcccgataac ctggaagaag
     4921 aacatcttct ttatcaaatg cgtaagcact agccagctga cttgcctctt gtccagcagt
     4981 tggtgcaaaa aagcctaggc gtccttggcg gttcagcgct gttgaacgtt ggtctaatac
     5041 acgagaccat accatatgag tcattaattc aacgagttca tcatcggata aatcaggtac
     5101 taaatctttg ttaactacgt ttccatcttg atcaagtgct tgatatactg ggaaatctgc
     5161 attgacttca tctagcagtg cttggaagtc gatcggtgct tttttgtttg ccatttgtca
     5221 cacattcctc tctcatctat aagaaaatct cttcattctg cgattgaaac agtgaataaa
     5281 actgtatcac tatttaaccg tcttcattaa taaattatca tggatttatg agaaatacaa
     5341 gctaaaagca caaattttca cgatgaaagg aaattatgcc aatccgcata ttaacagcat
     5401 tagtgaataa aatcacaata aggaaagcgt tttatatttc atctgcattt tttctatttt
     5461 ctaatcaaac ttacaaccat ctgtttctat ttaaaaacgt tggtgttata cagttttgtt
     5521 gcaaacattt attttattta cacatcgtga acggacgtac aaataatggt tttttcttag
     5581 tttctttata tcgtattctt tttccgaaaa aaaagaaaca gcttgaaatt taaatccaag
     5641 ctgttatatt ctttaatcta tttactttgt gaaattattc tgttaaccac ttttctagaa
     5701 ttttcatctt tgaataaaac aacggtattt ggttttcatc cagcctactg taattgattc
     5761 tgattccatt tatatcttgc ccaaaaagta gacttggtgc aattccaaga cccatatcta
     5821 ctactttttc tgctagttcg gggtctagtt ttttcccatc ccaagtaatc caggcatagt
     5881 aaccacctaa aattgaatga agcttgaaaa acgattgtga atctccccaa gcgcttatca
     5941 gtcggctttt ttctttcaac ttttttctta catcttgcat tttcaaatca attgcttcat
     6001 taaatacatt agccactgtg atttgtgtaa ggatattcat cgaaaactcc atcatttctt
     6061 gcgcttgagc tagttgcgag ataaaagtct caggtcctat cacccaaccg attttcgttg
     6121 tttttcctaa aaccctagat aacgaaccga cgtataacac attatccgga tcaatcgttc
     6181 gtataggaag gacttcttct tggtttagaa atgaaagatc cgtaaataca tcgtcttcaa
     6241 taattggaag ccgatactgt cgacacaatt ctactagctt ttctcgtcgt ttttgactca
     6301 tcacttttcc tgtagggttt tgaaaattcg ggttgataag cactgctttc aatttatttt
     6361 tatggatcga ttctttcagt ttttctaagt cgatcccttc ttcatccatc ggtatcccaa
     6421 ataaacgcgt tcctgaagct ttgaaaagag gtaatcggta aaaaaaagaa ggtgactcta
     6481 cagctatccc ctctccagct gaaagaatcg tttgtaaaat caaaaagatt gcttgctgac
     6541 cgccaccagt cacaagaagc tggttggggg aaaatgccaa ttcaaattta ctttccaaat
     6601 accgtttgat tttgttacgt aacggagtat agcctaaaag atccatttct ttttcttccg
     6661 ctaagatttc ttcaagagaa taagaaggaa actcaaaatt cggcaaaagc tcatctggca
     6721 tctcgctagc gtataaatca agaaacgatg ttttttggat ctgctttgct tctcttattt
     6781 tttgctcata ccagtccgtc tgctcataac gaggagagat catttgccgc caatcgattc
     6841 ttggaacagc gaatttcccc cattcagtct gattaacaaa ccatccgctg ccacgtttgc
     6901 tcgttagtac cccaagctcc ctaagctctt ccaatgcatg gacaacggtg gaacgattca
     6961 caccaaaaag ttgccctagt tttcgttctg attcaagttg acttccagga ttgatccttc
     7021 cgttttcgat ttctctcata atctgttcca taatttgacg ataaatcggt ccttgttttt
     7081 tctctacttt ttcccacata agcttctctt ttctattgtc tattttttct tttgatttta
     7141 ccgaatttac gagaaaaaag aaaaactttt tttgcaaaac ataagggact gtgacaaaaa
     7201 tcttatcaca gtcccttatt ctgaataatg atgttcaaaa gcttgtatct
//
LOCUS       DAXWLF010000132         7225 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXWLF010000132 DAXWLF010000000
VERSION     DAXWLF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.134"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            70..153
                     /locus_tag="ACGE4U_002136"
     CDS             70..153
                     /locus_tag="ACGE4U_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFF8394257.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            278..1015
                     /gene="erm(B)"
                     /locus_tag="ACGE4U_002137"
     CDS             278..1015
                     /gene="erm(B)"
                     /locus_tag="ACGE4U_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFF8394258.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1185..1445
                     /locus_tag="ACGE4U_002138"
                     /pseudo
     CDS             1185..1445
                     /locus_tag="ACGE4U_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1608..2273
                     /locus_tag="ACGE4U_002139"
                     /pseudo
     CDS             1608..2273
                     /locus_tag="ACGE4U_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2309..2554
                     /locus_tag="ACGE4U_002140"
     CDS             2309..2554
                     /locus_tag="ACGE4U_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8394259.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2657..3526
                     /locus_tag="ACGE4U_002141"
     CDS             2657..3526
                     /locus_tag="ACGE4U_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8394260.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3507..4241
                     /locus_tag="ACGE4U_002142"
     CDS             3507..4241
                     /locus_tag="ACGE4U_002142"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8394261.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4274..5182
                     /locus_tag="ACGE4U_002143"
     CDS             4274..5182
                     /locus_tag="ACGE4U_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFF8394262.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5179..5721
                     /gene="sat4"
                     /locus_tag="ACGE4U_002144"
     CDS             5179..5721
                     /gene="sat4"
                     /locus_tag="ACGE4U_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFF8394263.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5814..6608
                     /locus_tag="ACGE4U_002145"
                     /note="aph(3')-IIIa"
     CDS             5814..6608
                     /locus_tag="ACGE4U_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFF8394264.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6884..>7225
                     /locus_tag="ACGE4U_002146"
     CDS             6884..>7225
                     /locus_tag="ACGE4U_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8394265.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEK"
BASE COUNT         2525 a         1087 c         1514 g         2099 t
ORIGIN      
        1 tttgtgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
       61 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
      121 cagactaaaa acagtgatta cgcagataaa taaatacgtt agattaattc ctaccagtga
      181 ctaatcttat gactttttaa acagataact aaaattacaa acaaatcgtt taacttctgt
      241 atttatttac agatgtaata acttcaggag tgattacatg aacaaaaata taaaatattc
      301 tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa
      361 agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc
      421 taaaataagt aaacaggtaa cgtctattga attagacagt catctattca acttatcgtc
      481 agaaaaatta aaactgaaca ttcgtgtcac tttaattcac caagatattc tacagtttca
      541 attccctaac aaacagaggt ataaaattgt tgggagtatt ccttaccatt taagcacaca
      601 aattattaaa aaagtggttt ttgaaagcca tgcgtctgac atctatctga ttgttgaaga
      661 aggattctac aagcgtacct tggatattca ccgaacacta gggttgctct tgcacactca
      721 agtctcgatt cagcaattgc ttaagctgcc agcggaatgc tttcatccta aaccaaaagt
      781 aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa
      841 gctatatacg tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa
      901 aaatcagttt catcaagcaa tgaaacacgc caaagtaaac aatttaagta ccgttactta
      961 tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta
     1021 tgagtcgctt ttttaaattt ggaaagttac acgttactaa agggaatgga gataaattat
     1081 tagatatact actgacagct tccaagaagc taaagaggtc cctagcgcct acggggaatt
     1141 tgtatcgata aggggtacaa attcccacta agcgctcggg accccttgta ggaaaatgtc
     1201 ctaagtgtgg caacaatatt gtattaaaaa aatcgtttta tggttgttca aattatcctg
     1261 aatgtaagtt tactttagct gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa
     1321 aagaattact ggagggaaaa gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc
     1381 cctacaatgc cgttgtaaaa attggggaaa agggatatat tgattttatc tctttttcaa
     1441 aataaacatg gggctgaccc aaaagtagct cctatatgaa aaatagaggt ctttccattt
     1501 agatagtagt ggaaagacct ctatttatct tttgaaagtg aatacaaaaa taaagcacac
     1561 tctgctataa tataggtgat taatccattt gagagaggtg ctttatcatg tatcaaaatt
     1621 ataccataaa ccaactttgt ttgcctatag atcttgaaat taaattagaa gaaaatgatt
     1681 ttgctcatgc cattgtgcag tttgttgact ctattcccga tgaagtcttt cttccttatt
     1741 atcaatctat gggtagaccc caatatcatc ctcgtatgct actttccatt attctttgtg
     1801 cctatattca aggggtttat tcgggccgcc aaatacaaaa tatgctgata gatagtattc
     1861 ggatgcgcta cttatctcaa gagcaattcc ctaactttcg tactattaat cgttttcgcg
     1921 ttcatccgat tatgaacgat attctagatc attcatttgt tcaatttcgt gagctactgg
     1981 ttcaaagtgg cttaatcagt ggcagtgctc tatacattga tggtacaaaa attgaagcgg
     2041 atgcgaataa atactctttc gtttggaaaa aaagtatttt aaagtataag gatagccttg
     2101 atcaaaaagc ccttgaaaac taccaacaaa tggtggaaac aaaaatctta cctgagttaa
     2161 tagatgattt aaaggatgaa ctttctatag aagagattga aaggattcgt caatctttgg
     2221 aagaaaagga aaatcaatta attgaagcga ttgaccaaac cgaaacagta gaagggggct
     2281 gacccattat taataaggag tggacaacat gattgatgta aatgagctat tggaagaagc
     2341 tattagagaa actgaaaatc taaatgatgg cgaagttttt cttgttaaag atttattcaa
     2401 gggatatgtg gggaatagga tacccagaaa ggatcgactt ctgcttggaa cgttattttt
     2461 aaaccatgtt agcaaaatga atggtaattt aaaggctatt gaaaagactt catctaatca
     2521 gcagaggtat aaaaagacaa ttgataagtt ttaactatct tttgtgaaat taaaaaaatt
     2581 ataaatatgt tgagtagaag caaaagattt ggcttggaat ttttacagca gtgaatcgat
     2641 aaaaggagca tgcttgatgg tagataatat tattaaatca gtagcagaga aattatcctc
     2701 tctgtcttat atagaaggta ttgttttagg tggttcacgt gcaaggggca cccatacaga
     2761 ggattcggat atagatatcg gcatctatta caattcagaa tcatttgaca taaatactat
     2821 taatcaattc gctacaaagc tggatgatga gcatagaaat aaccttgttg tacctcccgg
     2881 agcatggggt gattggatta atggcggcgg atggttagtc ataaacgggt atcatgtgga
     2941 tttaatttta cgtgatatta aacgtgtgga acaaataatg aaagatacag agcacggaat
     3001 tgttactgcc aattatcaga ctgggcatcc ccatggttat attagtgcaa tgtatcgagg
     3061 agaattagcg attagcaaaa tactatatgc taagaatgaa agcttatgcg aattaaaaaa
     3121 acaggcagaa acttatccca atgctttgca gaaaagttta gttaactttt ttatgtttga
     3181 agcagggttc tctttaatgt ttgtaaaagc aaattcggga acagacgata aatattatat
     3241 tgcgggtcat gtttttcgta tagtttcatg tttaaatcaa gtgttatttg catgtaataa
     3301 tgcttattgt atcaacgaaa agaaagctat aaaactgctt gaaacttttg aacataaacc
     3361 tgaaaaatat accgagaagg taaatcatat ttttgaagta ctcggtatct cactttttga
     3421 atgctacgac atgaccgaga agctttataa tgaagtgaat gaaattgtat cggagataaa
     3481 taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat aatatatttt
     3541 ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg ggagaatggg
     3601 agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat ttaggatgcg
     3661 gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg
     3721 atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat
     3781 atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa
     3841 gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga
     3901 tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg
     3961 gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt
     4021 attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata
     4081 gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg
     4141 tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac
     4201 gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac
     4261 gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga
     4321 acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc
     4381 taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag aaccgtttat
     4441 atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa agccggagga
     4501 tatggaatta ttcccacctg aagaaaaggg attttcctat cttatgctat ttgatgatta
     4561 caataaaatt gatcttacct tattgccctt ggaagagtta gataattacc taaagggcga
     4621 taaattaata aaggttctaa ttgataaaga ttgtagaatt aaaagggaca tagttccgac
     4681 tgatatagat tatcatgtaa gaaagccaag cgcaagggag tatgatgatt gctgcaatga
     4741 attttggaat gtaacacctt atgttattaa aggattgtgc cgtaaggaaa ttttatttgc
     4801 tattgatcat tttaatcaga ttgttcgcca tgagctgctg agaatgatat catggaaggt
     4861 cggcatcgaa acaggcttta aattaagtgt aggcaagaac tataagttta ttgaaaggta
     4921 tatatccgag gatttgtggg agaaactttt gtccacctac cggatggatt cctatgaaaa
     4981 catatgggaa gcattatttc tatgccatca attgttcagg gcggtatccg gtgaggtggc
     5041 ggaaaggctt cattatgcct atccggagta tgataggaat ataacaaaat ataccaggga
     5101 catgtataaa aaatacactg gtaaaaccgg ctgcctggat agcacatatg ccgctgatat
     5161 agaagagagg cgggaacagt gattacagaa atgaaagcag ggcacctgaa agatatcgat
     5221 aaacccagcg aaccatttga ggtgataggt aagattatac cgaggtatga aaacgagaat
     5281 tggaccttta cagaattact ctatgaagcg ccatatttaa aaagctacca agacgaagag
     5341 gatgaagagg atgaggaggc agattgcctt gaatatattg acaatactga taagataata
     5401 tatctttact accaagacga taaatgcgtc ggaaaagtta aactgcgaaa aaattggaac
     5461 cggtacgctt atatagaaga tatcgccgta tgtaaggatt tcagggggca aggcataggc
     5521 agcgcgctta tcaatatatc tatagaatgg gcaaagcata aaaacttgca tggactaatg
     5581 cttgaaaccc aggacaataa ccttatagct tgtaaattct atcataattg tggtttcaaa
     5641 atcggctccg tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt
     5701 ttctggtatt taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa
     5761 ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta
     5821 aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata
     5881 cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt
     5941 taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca
     6001 tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg
     6061 atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg
     6121 aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc
     6181 actccatcga catatcggat tgtccctata cgaatagctt agacagccga ttagccgaat
     6241 tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca
     6301 ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg
     6361 aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag
     6421 taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct
     6481 tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg
     6541 acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat
     6601 tgttttagta cctagattta gatgtctaaa aagctttaac tacaagcttt ttagacatct
     6661 aatcttttct gaagtacatc cgcaactgtc catactctga tgttttatat cttttctaaa
     6721 agttcgctag atagagttct atattagaga tgtaaagagt tttggtgaag aggtttgtga
     6781 gtatggcttt tataaccagg gtgcaatgag aagcactaac agcagctagc ttaagaacgc
     6841 ataggatata ctttttatgg aagtcacaga ggaggaataa aatttgaaca ggattaacag
     6901 agtaacctct attcttattc agctgcaatc aaaaaaaata attcctgcca aagaaattgc
     6961 acagcgattt aatataagct taaggacagt ttacagagat atccggacac ttgaagaagc
     7021 cggaatacca attggatctg aggccggaaa aggatatttt cttgtagagg gcttcctact
     7081 tccgccggta atgtttacag cggcggaagt gggtgcattg attacagcag ggaaattttt
     7141 aaattgccat ggagatgaat catttataaa ggattttgat tcagctatgt ataaaatcaa
     7201 atctattttg aagcatggtg aaaaa
//
LOCUS       DAXWLF010000133         7136 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXWLF010000133 DAXWLF010000000
VERSION     DAXWLF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.135"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..430
                     /locus_tag="ACGE4U_002147"
     CDS             <1..430
                     /locus_tag="ACGE4U_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFF8394266.1"
                     /translation="QNADFIDSLSVENKKLDEILIESLIEKEWLYQVWGDNIVNCLKL
                     VGNNDYSQFYDWYKKFKYAIKSAEKEQKIQLKIIYEICNNKYFVDYVREQLSMTLRDL
                     IRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            506..724
                     /locus_tag="ACGE4U_002148"
     CDS             506..724
                     /locus_tag="ACGE4U_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394267.1"
                     /translation="MIENRLIDQVWCLDQNTDNLELIETICFNTIVLDLRSENDNCVT
                     HIIIDRKTAQKLSESLRKWAEGGNNENS"
     gene            711..3404
                     /locus_tag="ACGE4U_002149"
     CDS             711..3404
                     /locus_tag="ACGE4U_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFF8394268.1"
                     /translation="MKIVKEKIASRKIHWLALSILVLMFIVVFTGNVVQASIFNWFDD
                     TESQTEFLNNPVYQSYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFIESTGMNKVYQSVLSTVVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     TIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISANIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNNGGNDQAEGEERKPERNFGQSLSKKANKTGKALGYAHERGLSGLASDGSTMAS
                     ERATKPFKSMRDIANDTKSKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADSIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAK
                     NSNTGLEKLVKENPQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQG
                     TKTIDVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDV
                     RENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIID
                     SEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDSLLKK"
     gene            3418..3615
                     /locus_tag="ACGE4U_002150"
     CDS             3418..3615
                     /locus_tag="ACGE4U_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394269.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNEIESDFKCCSLEKNVRG"
     gene            3619..4230
                     /locus_tag="ACGE4U_002151"
     CDS             3619..4230
                     /locus_tag="ACGE4U_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFF8394270.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4215..5606
                     /locus_tag="ACGE4U_002152"
     CDS             4215..5606
                     /locus_tag="ACGE4U_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFF8394271.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5822..6136
                     /locus_tag="ACGE4U_002153"
     CDS             5822..6136
                     /locus_tag="ACGE4U_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394272.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6129..6623
                     /locus_tag="ACGE4U_002154"
     CDS             6129..6623
                     /locus_tag="ACGE4U_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394273.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTIDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKEETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFTL"
     gene            6700..7014
                     /locus_tag="ACGE4U_002155"
     CDS             6700..7014
                     /locus_tag="ACGE4U_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394274.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDRIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSETFEIDPDYYQPQEVFREFRNQIIRSSNI
                     EN"
BASE COUNT         2707 a          953 c         1394 g         2082 t
ORIGIN      
        1 tcaaaatgct gactttattg attcactttc agttgaaaat aaaaaattag atgaaatatt
       61 aattgaatca ttaattgaaa aagaatggct ttatcaggta tggggggata atattgtaaa
      121 ctgtctgaaa ttagtgggta ataacgatta cagtcaattt tatgactggt ataaaaaatt
      181 caaatatgcg attaaatctg ctgaaaaaga acaaaaaatt caactgaaaa ttatctatga
      241 aatctgtaat aacaagtatt ttgtagatta tgttagagaa cagctatcaa tgactttacg
      301 tgacttgata cgaagagcaa aaacagatca ccgtatcaaa caaaaagacg attatatctt
      361 tgcttctcta aaaaataaag cgttagaact aattgaattg caaatcagtg aaggaataga
      421 taagcaataa gaatagaatt gagtcaaatg aagtggaggg gtatctccgc ttcatttgag
      481 aactttgaat ataggagggg ttattttgat tgaaaatcgt ttaatagatc aagtttggtg
      541 tctagatcaa aatacagata atctcgaatt aatcgagact atttgcttca acactatagt
      601 tctcgattta agatctgaaa atgacaattg tgtaactcat atcataattg acagaaaaac
      661 agctcagaag ctctctgaga gtctcagaaa atgggctgag ggaggtaata atgaaaatag
      721 ttaaagaaaa gattgccagt agaaaaatac actggttagc tttgagcata cttgttttaa
      781 tgtttatcgt tgttttcacc ggtaatgttg tccaagcaag cattttcaat tggtttgatg
      841 acacggaaag tcaaactgaa tttttgaata atcctgtcta tcaatcgtat ttaaaaaaat
      901 ataacggaaa tttttacagt cagtttggat tgtggatagg gtggtctgtt ataaaatcga
      961 tgtttgcaat tgtagatggt attcaaaata tgattcccga agtgttagat ttgtttagct
     1021 ttattgaaag tacgggaatg aataaagttt atcaatcggt cttaagtaca gttgttattg
     1081 gtttaatgat actttcactg atgtttgttg gttacaaaat gattactgga aaaggaacag
     1141 tggatttaaa atcggttgga atgaacgtag tgatgtctgt agcattaatt cttttaatgc
     1201 cgactttgat tggatcaaca gaaaaacttt caggaataat gtttgcaaag actctttata
     1261 atgacgcaac tagtatcaat aatcaagatg gcggtgtagc atggtcattg attgagcagg
     1321 gagtgacaga tttagcttat attaataaga cagatcaata ttcgacaatt gataaaacaa
     1381 atgaccgaaa taatctaaca aaagaaaatt tctggaaaac ggatcttacg caagtattga
     1441 cagataatgt aattgataaa atggaaaaag agaacccaac agctgataat ctgcgttatg
     1501 aattagttga aaattctgat aatgaatttg ttgctactaa attttcagat aattttttat
     1561 caacctttgt tgatggtatc aaatcaggat attatcgcta tcaagcgaac ctgtggggaa
     1621 tttcttttgg attgattgct ttagcggtag cgtatgtttt tagtgctttc attataatta
     1681 cagcaatact cgagttagca tttaaacgtg tactcggagt tttagtattt gctactgact
     1741 tagaaacagg acaacgatca aaacaagttt tgtcagatat tttacagtgc tatttgacag
     1801 tcggatttca agggttcggt ttatctatgt ttgcaatgtt cataaattac ctaaatactg
     1861 gcgaaggtat ctcagcaaat atttttataa aaacaattgc ttatatctgt gctgtgtttg
     1921 ttttaatcaa aggcagtgga acagtcatga gatactttgg tgtggatatt ggactcaaag
     1981 aaggctatgg gcaacttgct tctgcttttg gaatgggtgc aatgctattc cgtaaaggaa
     2041 caggtggtat gaaccggtct aaaggtggga aaaataatgg tggaaacgat caagctgaag
     2101 gggaagaacg aaaacctgaa cgcaactttg gacaatcact ttctaaaaaa gctaataaaa
     2161 cggggaaagc gcttgggtat gctcatgaac gtggattatc aggtttagct tcagatggat
     2221 ctacaatggc tagtgaaaga gcaacaaaac catttaagag tatgcgtgat atagctaatg
     2281 ataccaagag taagtttaaa gaaggtttag atgatggtac tgtgtccgct attaataaaa
     2341 atagcaaacc tatgttagct aaaaataaag aagatgaaac tggaaaatat gctgattcca
     2401 tgccaacacg tctttcggat agaaaagatg gtgctaaggt tgaaaatgcc gatagtatta
     2461 tgtctagttc tgaacgtatg cgtgaagcaa tgaaaaataa tgctgaatcg aataataatc
     2521 ctgtaagtgc aattcagcaa aaagtccaac aagatattga agaacgtaaa aatgctatgc
     2581 acggtcaagc taagtctgct gaagaattaa ttaatcagaa gcgtcatgaa gcgaaataca
     2641 cgcctgaagc aatgaatcgc gaggaaataa tgttaaggaa acgtgtagaa gggcggacag
     2701 gcgctaatat tgacggaaaa gaagcgttga ccaaagaaag aattcaagaa gctaaaaatt
     2761 caaatacagg attagaaaaa ttagtcaaag aaaatccaca aaattctaca agtagtcatg
     2821 gtggccgatc agtagatgtc cgagaaaata ttcaaggttc actgaatggt catgttggac
     2881 ggaatgttga tgtaagagag aatttacaaa gttcttcaaa tgctcaaggt actaagacaa
     2941 ttgatgttcg agaaaatctg caaagtagca gtacaagtca accaaaaaca gtagatgttc
     3001 gagaaaatgt acaaaattct acaagtagtc aagggtttaa atcagtggat gtccgagaga
     3061 atgtgcaagg tagcagtgca agtcaaccaa aaacagtaga tgttcgagaa aatgtacaaa
     3121 gttctacaag tagtcaagga ttcaaaacag tagatgttcg ggagaacgtt caaaaagata
     3181 gtggagaaat gaaagaaaaa acacaaaaaa tcaatatcgt tgaagacaga aagtcagcga
     3241 aaaaatttga ttcaaatcat gaaacagtga ttattgatag tgaaatccgt gaaaatgata
     3301 agggatcaaa accaagacga aggtttacct atgaagacaa cgagttattc agagatacac
     3361 ttaatgatcc taatccactt ttcgatagtt tacttaaaaa ataaagaggt gattgtcatg
     3421 caattaattc tacagattag tacacatgat ttcttagaac ttgacgaatt atgtgaatca
     3481 gtaaagaaag caacaaatga aaaaatcacg gtcactaatt atattgaact gatgattcaa
     3541 gaagaattaa agatgaaaaa tgaaatagag agcgatttca aatgttgctc tctagaaaaa
     3601 aatgtgaggg gataatcaat ggatgataaa caaaaaatgt acgaaaaaaa tcgcaaacat
     3661 agtgaggcct taaattttcg tgtcgataaa aagacttatc ttgaactgac attgattgca
     3721 aaacaaaggg gaatttctag agcagacgtt tgccgtgagc ggttaaaggg actcaaatat
     3781 gaaccgccgt tgttagctga agatgatagg gtagttttaa ccaaacagtt aaatcaaatt
     3841 ggtaacaatt tgaatcaagc ggtaaaggca ttaaatatca ttgctaaata ttttagagaa
     3901 gaatctgaag ataaaaaaga aaagagccta cattttagtt ttgatgctag ccgttacaac
     3961 atttttaaag atgaacttgc gaatagcata gcaaaaaagt tgatgattag ttatccagat
     4021 gacatttttt tggagtccaa tcggagtgcc ccctcttttt ttaaattatc agaagtagaa
     4081 aaagaatatt tcgaatattg gatagaaaag caaactagac cgggtacgaa agaagagtat
     4141 tcccaaaaag ttgatcggaa tttaattcac tcaattcaga ttattgcaag tttaaaagat
     4201 gaggtgaaac aactttggga gctactataa catcgaaagg acgcaagaaa aatttatcct
     4261 cgctattaca atatgcagct aatccttcaa aaaaagaaaa atgtgttgca gtctcaggaa
     4321 taaattgtag tgatgatagt gaggaagcac tgcaagaaat gaatttagta cgtaccaaga
     4381 tgaataaatc taaagatata gcacaagcgt ttcatgttat tcattcgttt gataagagta
     4441 cttctaaaga gttaagtatt gataaaatgc acgaaatagc tgtcgaattt gcagagaaag
     4501 cttttccaaa ttcgcaaatc attgttgcaa gtcacaatga taaagatcat tttcatagtc
     4561 atcttgtcat taataatatt aacatggaaa catgtaaaag gttgcgtatt gaccctaaag
     4621 atttagaact aatgcgaaaa attaacgatc aagtcctcaa tgaacatggt cttgaatcag
     4681 tgaataatga gtactatgaa aacttagaaa agagaaccaa tctttattca aacgatcctt
     4741 ctaagatgaa atatggaaaa attaattatc aagagtttgt gcaaaatgcg atcgaagcaa
     4801 cgttggaaaa atgcgaagtg aattcttttg aagatttttc ggatcatttg gcaaaagaat
     4861 ataacattga agtttatcaa ttttcaaaaa caaataacaa acttggctat gttctttaca
     4921 aacaaggtgt aaatttctat gagcgtagag gtgagttaat tactttagga agagataaac
     4981 aatctagaaa agaaaaaagt cagtatgtcg aacgtacttt tagtgcaaga aagttaggga
     5041 aaagatattc atttgaatct gtttctgaac aacttgaaaa gaatttagaa gaatcacata
     5101 taaaaaattt ttttaatatg gaaagagagc aagttatgtc gctaattagt ggagtaaatt
     5161 acaaaactgg tgagttaaat gtaccttttg tggaattaga ttccatcgca caaaagcaat
     5221 ttagcgaccg tgaaggcgat gaaaaattca ttaaatatga tgacttcttg cgagaggtag
     5281 acaagcattt tgtaaacaca atgtctttag aagatgatca agaatacacg attaaacttc
     5341 atacggatgt gaatcctcaa actttacgta gtgcgaaaag taaaacaatt tctattaaaa
     5401 agactgatca gaaagagtta tttctaccgg caaagggttt tgaaaattgt tctcatacac
     5461 cttctgctaa acagattcac tatcaaatca gtcgcataat gaatgacgat gtaaaaatgt
     5521 atcgtgctaa aaaggattat gaattcacac aacaaatgat tgaaaatcag caaaaaaggc
     5581 aacaaaattt tgaaatggaa ttctgaatgg gcttgttagt ggactttcca gttttttgcc
     5641 tatgaagagt tgcttggaaa gaagcagttt ttaggacata cgccagctac ccatctactt
     5701 gccagtcaat ccgccccgct cacgctaggc tactttctgg caagtcagat ggtgggtaaa
     5761 tctctgaggg agcaagctcc ctacagactc cctcccaaaa aaagaaaggt ggaagaaaaa
     5821 aatgaactat ccatatttta aagtctcggc ttcagaagaa actaaagaaa tattcaacaa
     5881 tttttacaac caaaacaaag gagtttttgg ttccaaggca aatatgtttc gtgttatggt
     5941 ttcaaattta ccagtgttag ctagtccatc aaataataaa tttaatgatt ctgaaagcat
     6001 taaatttgaa caaaaaattt cagaactaga atcaatgata agtaatgaag taattgaaaa
     6061 gcttgatgac attgatcaaa agttatcgta ttctctaaaa aacaaataca aaacggagga
     6121 gaaaaaagat gtttaaagtt tacttcccgt ttcagtggct cgttaaagga attcttttag
     6181 tgctactagg attgattctt tttggaagtt atctaacaat taaagccaga cattttccag
     6241 aagaaaagcc aataaaaatt gtaacaatag atttaaaaat aggtgaaact tatcaggcag
     6301 atggggttga actaacattt aaaaatattg aagtgaccga aaaaagaaat gaatttgcac
     6361 aacatgatcg tgtattacaa gtgacctata cacttaaaaa tgatacagaa aaaccaatac
     6421 gtccacaaca atggtcgatt gaaaataagg atggtgaaga gctttatgag tatgctatga
     6481 tattagataa tccagagtat attgaaagtg acaaagaaga aacagtaaat tactattatg
     6541 aattaccgat tgaaaggcag gactaccaaa tcgtagtacg tagagcgaat ggttattttg
     6601 ttggattacc ctttactttg taagtcttat tttgttagtg gcttttaagt taatatcatt
     6661 tttgtgattt ttgaaaatga aagtgaggaa attggaagaa tggctgaaga aaataaagtt
     6721 gttccgtttg aaacattagt gataaataag gaagagtttg agcaactttt tcttgatttt
     6781 tttattcaat atgacaggat ttatgatttt agttcgtcac tagatttatt tgaattagaa
     6841 gcagaaataa tagaaaacag tcgagagaag aatggtaaaa tcattatttc agtagaaaaa
     6901 ttcaatgaat tattctcaga aacatttgaa atagatccag actattatca accacaagaa
     6961 gtttttcgag agtttagaaa tcagattatt cgttcaagta atattgaaaa ttgagtcaat
     7021 aaaagaagcg ttgaagaaaa gttccttctt tcgacgcttc tttttaaaag attttacaaa
     7081 tgtcttatgg agatttgctt caatgactac ttgtagtcat ttcgtttaaa ataatt
//
LOCUS       DAXWLF010000134         7131 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXWLF010000134 DAXWLF010000000
VERSION     DAXWLF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.136"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(91..609)
                     /locus_tag="ACGE4U_002156"
     CDS             complement(91..609)
                     /locus_tag="ACGE4U_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFF8394275.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(611..1228)
                     /locus_tag="ACGE4U_002157"
     CDS             complement(611..1228)
                     /locus_tag="ACGE4U_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFF8394276.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1225..2079)
                     /locus_tag="ACGE4U_002158"
     CDS             complement(1225..2079)
                     /locus_tag="ACGE4U_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8394277.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2076..2987)
                     /locus_tag="ACGE4U_002159"
     CDS             complement(2076..2987)
                     /locus_tag="ACGE4U_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8394278.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLTPKWVYYSGGDGK"
     gene            complement(3028..4362)
                     /locus_tag="ACGE4U_002160"
     CDS             complement(3028..4362)
                     /locus_tag="ACGE4U_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8394279.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGISDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4570..5400
                     /locus_tag="ACGE4U_002161"
     CDS             4570..5400
                     /locus_tag="ACGE4U_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFF8394280.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5644..5893)
                     /locus_tag="ACGE4U_002162"
                     /pseudo
     CDS             complement(5644..5893)
                     /locus_tag="ACGE4U_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6084..6278)
                     /locus_tag="ACGE4U_002163"
     CDS             complement(6084..6278)
                     /locus_tag="ACGE4U_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394281.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6783..6968)
                     /locus_tag="ACGE4U_002164"
     CDS             complement(6783..6968)
                     /locus_tag="ACGE4U_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394282.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT         2352 a         1415 c         1224 g         2140 t
ORIGIN      
        1 caaatgaata aacaactacg ctttcctcga aacaaaaaaa taaagcacaa atgcgcattt
       61 acgcactcat gctttattcg tacaattaat tcagataacg gcagcgcata ggatgccaat
      121 aacacttcct aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa gtaaaaaaat
      181 cctcgaataa gcgatcaaac aagcaaataa aaaggctaat attcctgcaa ctggacttaa
      241 ttcgaacaac gcagctactg ataaaaaggc tagcgctgta tgtcctgaag gaaaagaaga
      301 agtcttcggt ttacttgtgt aaaggacaag ccgacgattt ttttcataag gtcttttccg
      361 tcttgtcgtt tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac tgccgataag
      421 aatggctaga ccatattctc tagtatcagg tatcaagact aaaacagcag caaggagcaa
      481 ccaaataaaa ccaccattag agatattact gataaatttc aaaaaattga tttttcgact
      541 atccgttttc cgttgcttgg cgaacttgtt gataatacta tcatcgattt tagcaatcaa
      601 acgcaacatg ttattcctcc tttttaagaa ccttttcttg cgtacgatgg ataaaaagca
      661 ggacttgaaa ataaaacaag aaactgaaac ctaataaagc aaaatagatt ggtcctgcaa
      721 accagaatgc gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg cttttgaaat
      781 cattaaaaaa caagaaaaat gcagtaatcc aagaagcttc tttttgttcc agttgaagca
      841 agattttgta actaaagaga agcgcgctta atagaagcaa aaacaacgta gtatacatca
      901 atgtatagcc aattccccca ccccaatagc ggaaaatctg ataatcgatc aatagaagaa
      961 gaaaataaac acttgctaaa ctatcttgct ttccataaag agagaagttc tcacgaaact
     1021 ttttccagaa tagtgaaccg agctttttct cttcattttt tttgtaatga taaatcatat
     1081 acagaactgt tcgacttgca gttcctaatg tgaaaacagg caagacgcac aggaaccata
     1141 gtagctgtaa atggataaga cgtggaatcc attcaatgat ttctaagaaa taatcattgg
     1201 cttttttacc aatcgatcga ttcatcatcc ttttgaacct cctgtcaaat ccatcccttc
     1261 aatgaaataa cgttgtgcaa aaaagaacag caaaatcgtc gggatcaaga caagtgttgc
     1321 tcctgccatc agataattcc attgtgtcgt tgcttggttc tgaaaaactt gcaagccaat
     1381 ctgtaacgtg tatttttctt gatcttggat atataacaga ggacctaaga aatcattcca
     1441 agctgcgttg aaggaattga tcgcaatggt aatcaaggca ggtttcgtca atggaagcat
     1501 caaatgactc caaatataca aatgattcgc tccgtcgatt tcagcagctt cgatcaattc
     1561 attattgatc gataagtaaa attgacgcat cagaaatata ttgaacgcat tgccgaagaa
     1621 cgatggaacg atcaggggca aataagtacc tacccagccg atcttggaga ataatacata
     1681 ttgagggatc atcgttacaa accccgggat catcatcgta gaaagaacca aagcaaacat
     1741 cagctttttc ccaggaaaat caagcttcgc aaatccataa gcaataaaag aatttgaaag
     1801 gacattaccg atcactacta gcacagtgat gaataatgta tttcttgcat agcgccaaaa
     1861 aggaaaggcg gcaatcgtat ccagatagtt ttcccatacg attttttctg ggaaaaaggt
     1921 cggaggataa cgaacgattt cttccattgg tttcaatgaa gtaaacacca tccaaagcaa
     1981 tggaatgatc aaaatgattc ctattactgt catgaggaca taacttagta ttttttttgt
     2041 ttgccgattc acactggtct ttttgaaata atccatcatt tgccgtcacc tccagaataa
     2101 tagacccact taggtgtaag cttcaaattg acaaacgtca gaaccaacac gatcagaaac
     2161 aggatcattg acatagccat cgcataaccc atatcaaaag ctacgaacgc cttgttccac
     2221 atatgcagat tataaaaaag caaagaattt gcaggtccgc ctgttccatt tttcgtcatg
     2281 acataagctt cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac atcgtaaaaa
     2341 ataataggag tgattaatgg caaagtaata cttttgaact ttccccaaac gcctgctcca
     2401 tcgatttctg ctgattcata taaagatcta ggaacatttt gtaatgtagc taagtaaagt
     2461 agcattgctc ctccagaact ccatattttc atcaaaatca acgcaggttt cgtccattgt
     2521 ggatcaaaca gccaagctgg tccagggata tggaaccagc ccagaaatgt gttgatcaat
     2581 cctgcagatg gagaaagcag ctgcatccat aagatgtaga ctgctactcc tgataagact
     2641 gccggtaaat agaaagctgt acggaataca ctgattcctt tgattttttg attgagtact
     2701 gtggctaaaa acacactgaa aatcgtcgtc agcggtacat tgaagatgac gaaataaacg
     2761 gtgttgtata acgatttcca aaataaatca tcgtgaacga acattctttt gaaatttgct
     2821 agaccaatga aattcatctt ggatgtcaaa ttataatcgg tgaaactacc aataactgaa
     2881 aaaagcatag gccctagcat aaataccaag aacccgataa taaaaggcga gataaataaa
     2941 taggcccatc ttgcttcttt tttctttcgg atagataaac gcttcaaatt ttcttgcctc
     3001 ctctgtacct agacagtcaa aaatggatta ttgattttgc ttgactaagt tttctactgc
     3061 tttttgtgca tctgccaatc cttcttttgg tgattttgtt cctaaaagaa tctggtcaat
     3121 ctgttcattc actaatgtcg tgtaatccgg agcatctaaa ggaactggtg tgatcaccgt
     3181 atttttaaag ttttcgtccg ccatttgata gatcattttt cctttatccg ttaggttgtc
     3241 attattcact aaattctcat tggctgtctt gctcgccatg atatcaaaac ttttttcgcc
     3301 gaatttttct tgtacttctg gagaagaaag atatttgatg aattcataag aggcctcagg
     3361 atcttttgct ccgtatggta cttccaagac gaatccgcca ccccacgacc aatggcctga
     3421 accttcttct ttttctggga gctgcatcac gccaaaatca aaatcatccg gtgcattttc
     3481 catcaaacta gagtaataat tgatattttg ggcacgcatg gctactaatc cggaaataaa
     3541 tggatcagat ataccagacc caaattcggc ttctaattta ttgatcgtat ttttcccata
     3601 ataatcttgc cagtcttgga tccactccaa ggcttccact ttatttttag tatcaatctt
     3661 gactttttca tctttgtcaa accaactagt cccgttatct gcgtttagcg cccatacatc
     3721 agcccctaaa ttccataatg gataaaaacc gatgcgtgta aattcttttc cttctttttt
     3781 atctagttta cgtgctgttt cttctaattc atcccaagtt gtcggtagat cttgttcact
     3841 gattccagct tctttaaata actttttatt ataaaaaatg acttgtgtat ctgtattgaa
     3901 tgggatcgca taaggatcac cttcatattc tactgtatcc caaagctgcg gataaaaatc
     3961 cttagaagtg tctttttcta catatttgga gagattagtt gcttgctgtg cttctgctct
     4021 ttgggcaacg gaatttatgt cctgtacaat aacatcgggt gcatttccag ctgttattgc
     4081 agataaagat ttagtccaga tgtcacccca tggctgatac ttataattta catggatctc
     4141 gttttgtgat tgattgaaat cctcaatgat ttcttcgata gcttcttttc gtgcaccgga
     4201 tccccaaaag gaccagaaat caatattttt tcttccatca cttgtttttt cagaagaatt
     4261 tgctgcactt gaactggcat cgcttgcatt acttgatccg catcctccta ataaaaacaa
     4321 tgctgaactt gccagcaacc ctgtaaacaa taattttttc atattttacc tccaaatttt
     4381 aatgtaaccg atgccggttt taatataacg atatcatttt gttctgaaaa taacaattta
     4441 taaggtatga ttctaaaaaa attattaatg ataccaatac tttatataat tcgcgagcgc
     4501 tttctttgaa ttttttaatc tttcgtagca gaatgagaat gtattaaaaa tagattagga
     4561 ggaattttga tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc agatcgtccg
     4621 aatccattaa tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg cgagatgagt
     4681 gtagctatgc agtacctgtt ccaaggctgg agctgccgag gagaagaaaa atataaagat
     4741 atgctgatgg acattgcaac agaagaaatc ggccacgtgg aaatgttggc gacaatggtt
     4801 gctcgtttat tagagggtgc gcctgttgaa gatcaagaag cagcgatgca aaaagatcca
     4861 acaatcggtg ccattatctc tggaatgaat ccacagcacg cgatcgtagc tggcttagga
     4921 cctcgtcctg ctgacagcgt gggcaatcca tggagcggtg cctatatcat tgcgagtggc
     4981 aacttgttag cagactttag agctaattta aatgccgaat cacaaggaag acttcaagta
     5041 gcacgtattt acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc tgttctcctt
     5101 gcaagagata gctatcatca aaatctatgg gaatgtgcag tgaaagaatt agaagaaaaa
     5161 gaaggttcaa tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa agacattgct
     5221 tatgacttct ataacttctc agagggagac caaagtagtg aaggttcttg ggctagtggc
     5281 aaagcactgg atggcgaagg cgaatatcaa tggttgaaag aacctaagat cgaaggcgct
     5341 gcaccaaaat tagaaccagt aacaccaaaa atgtatggga cacctccatt caagaaataa
     5401 atatctaccc gtagaaacac ccgttttttg ttccacacgc tttgtgagca aaactgacta
     5461 aagtcattaa tcataaccac ccttcaaaag ggtggttttc tctagagcta taagctcaaa
     5521 tcaccggcca gcgcctaaag acgctggctt tcatatttta tgttcaagcc atattgcctt
     5581 tgccagtttt gcccacctct taaaaggttt atgtactact ccttagccct taaaaggatc
     5641 gtcttactct cgtacactca gcttatctaa cgcactatca tgtttttctt gttcttgaat
     5701 gtactttttg atggtcgctt cattttgtcc tacttttgtc acatagtatc cttccaccca
     5761 aaagtgtcga tacccaaatt tatattttag atttgcacaa tctcttgatg atctcctgaa
     5821 tattggctcg atattgatta taaattattt ttcttctata ctttggtgtg aatacaatgt
     5881 ggtatttaca catctacttt gtacgggcta aactatttcc ctttattgct ccacccgcat
     5941 agcaagtggt tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa gacaataaat
     6001 ctaaaaaccc gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa tgaaatgaaa
     6061 aagatctctt acggcaatct attttatttt tttgattttt tataaatgga aaatgcttca
     6121 agaagtccaa gaaataaagc cgtataaaaa caagaaccta tgaaatcttt atttattaga
     6181 tattcaacag aaatcccgat aacactagcc aacaatatat ttatcagaag tgtaaaataa
     6241 ggatgctttt tatagaatag aactagtgtt gttttcatag actctccttt cttcatattt
     6301 ccaattaatc acaaattcac ggcattacta gaaaaattaa aggggcgcag aagtggaaaa
     6361 atactcttcg actttcccta caaaccaggc catcttgtgt ttaataacta taccaaatct
     6421 aaggtacctc taaatctaaa ttccatttgt ggcggtgacc tccaattttt aactcttcta
     6481 tctcccatcc agtatacata accttgatac ttcgaccact tatatatcga acacacaaat
     6541 acctatccat tgcaggagta ctacctaact tagttgctac ttgcatctaa ataacagctg
     6601 aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa ctatcatttt
     6661 tatcctcaac tacatgcaca tctgttgcaa cttcactttc tgtctcatcc agataaatga
     6721 ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag ctaaattagt
     6781 catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa caaacttgga
     6841 actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta tgattttagc
     6901 agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc tttagaattt
     6961 ttactcattc atagacactt ccttaaataa cttttattat agaaggtatt gtaaataata
     7021 atattgacaa aaagtgactc aaaataaaaa aaaacttaca ttatactgga atgaactgta
     7081 ttgatagtgt acctaaaagc tctactgaca taagatcgtg taatcggtaa a
//
LOCUS       DAXWLF010000135         7028 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXWLF010000135 DAXWLF010000000
VERSION     DAXWLF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.137"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(4..567)
                     /locus_tag="ACGE4U_002165"
     CDS             complement(4..567)
                     /locus_tag="ACGE4U_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8394283.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(622..801)
                     /locus_tag="ACGE4U_002166"
     CDS             complement(622..801)
                     /locus_tag="ACGE4U_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394284.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(878..1387)
                     /locus_tag="ACGE4U_002167"
     CDS             complement(878..1387)
                     /locus_tag="ACGE4U_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394285.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(1483..1863)
                     /locus_tag="ACGE4U_002168"
     CDS             complement(1483..1863)
                     /locus_tag="ACGE4U_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8394286.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            2193..2942
                     /gene="pnuC"
                     /locus_tag="ACGE4U_002169"
     CDS             2193..2942
                     /gene="pnuC"
                     /locus_tag="ACGE4U_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFF8394287.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(3126..4403)
                     /locus_tag="ACGE4U_002170"
     CDS             complement(3126..4403)
                     /locus_tag="ACGE4U_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394288.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4482..5126)
                     /gene="pcp"
                     /locus_tag="ACGE4U_002171"
     CDS             complement(4482..5126)
                     /gene="pcp"
                     /locus_tag="ACGE4U_002171"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFF8394289.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(5325..6269)
                     /locus_tag="ACGE4U_002172"
     CDS             complement(5325..6269)
                     /locus_tag="ACGE4U_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFF8394290.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(6271..6957)
                     /locus_tag="ACGE4U_002173"
     CDS             complement(6271..6957)
                     /locus_tag="ACGE4U_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFF8394291.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         2012 a         1479 c         1196 g         2341 t
ORIGIN      
        1 tttttagata ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac
       61 aactttgaag tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact
      121 tagaccgatg acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg
      181 cgaaccgatg ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc
      241 gttgggaaac gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc
      301 taagataggt accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc
      361 aactcctgca actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata
      421 atttggatca gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt
      481 caaaaatggc agttccatat cttttagaat cactaagatc aatggggcga tttctggagc
      541 atcttctttt ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca
      601 gaaaaagaaa tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata
      661 accatgagta acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc
      721 tttatcatta tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt
      781 agggcatttc attttttcca tgttatctca acttcctttc atctttactc tcttagctta
      841 ctcttatatt gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac
      901 ggtcctgttc tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa
      961 gtgtccactc tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg
     1021 cttgcggttg tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt
     1081 ccgtgtcatt tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt
     1141 cacaaaaaaa ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg
     1201 caaaatgaat agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt
     1261 ttttcaatga gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga
     1321 gcagtcccgt cataccggta atccctatta cgatgagggc agcaacacca aacatcattt
     1381 ttttcattct tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga
     1441 aaaatcgaat gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa
     1501 tggcaatccg tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct
     1561 aatcggttct gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc
     1621 tactttgaaa gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc
     1681 attagcaatt ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc
     1741 atctcgttct ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa
     1801 atcacgtgat ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt
     1861 catatgagca ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg
     1921 taaatgtgtc agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa
     1981 aaacagaaag taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa
     2041 gaaattatat tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg
     2101 aaagactagt tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac
     2161 actacattta gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc
     2221 atcagctaaa aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc
     2281 aggtcatgac tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta
     2341 ccacgcttgg tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt
     2401 taggtatttt gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa
     2461 gtatcggtga acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag
     2521 aatggaatca taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg
     2581 caatcatcag tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag
     2641 atgatcctcg gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca
     2701 tcagtttctt gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa
     2761 tggtcctttg gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca
     2821 gctctgttta cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa
     2881 aagaacgtga acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat
     2941 aaaaaaactg agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
     3001 taaacggcgg aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa
     3061 aagcaatttt cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
     3121 attttttata gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct
     3181 tctaacttct gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt
     3241 cctttccatt tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt
     3301 tgccattttt cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt
     3361 tctttacgca atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac
     3421 ccttctgtaa aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg
     3481 atgaatcgtt ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga
     3541 cgttttgtct ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc
     3601 acttcttcat atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag
     3661 aattcttcta catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc
     3721 tgtcgctttt ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat
     3781 gcttgataat ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga
     3841 gctaatttta tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa
     3901 cagtattttt gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat
     3961 tcgtctaagg ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga
     4021 gagacatgca ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt
     4081 ggcgatttaa cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc
     4141 tggcttcgtt ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt
     4201 gcagctaaat aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg
     4261 actagttccc gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg
     4321 tattgtttag aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca
     4381 aagacggatt gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct
     4441 actaacataa cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca
     4501 tttccggaaa tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa
     4561 gcaatgtctt ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt
     4621 gagaatggca cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga
     4681 agcaaacgat acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct
     4741 ggtagaccat gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa
     4801 gcggctggac cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg
     4861 attcttgctt ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct
     4921 gcttgtccga cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact
     4981 tgactgcttt tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt
     5041 tcatctggca ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga
     5101 tcaaaacctg ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg
     5161 gttatctttt atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa
     5221 taacataaca gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt
     5281 agaagaggtt gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc
     5341 atataagcaa tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact
     5401 ccgtatttgt ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc
     5461 ggtgtcagca gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga
     5521 ttcgctccat gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc
     5581 gcaaatgcat tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg
     5641 ccgatgttca catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca
     5701 actccagcgc ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac
     5761 ggccctactg tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct
     5821 cgagaaaaga acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa
     5881 gcacctaaat tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga
     5941 ataaaaattt tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc
     6001 cggctttctc ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag
     6061 ccaccgttca cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc
     6121 cagaaaaagg ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca
     6181 gtgtaaatca atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat
     6241 aaaatatcag gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt
     6301 cgctaatttc ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc
     6361 cactggaact tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag
     6421 tgttgattgg actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca
     6481 gcagacattt tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac
     6541 ttctaaccct tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa
     6601 tccagcaact ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata
     6661 aatatctaca atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga
     6721 ttctttcaga ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc
     6781 catcccgcga ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag
     6841 accgccagtc aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt
     6901 aaatgcaaac ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata
     6961 ttttcctcca taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata
     7021 gttgtttt
//
LOCUS       DAXWLF010000136         7013 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXWLF010000136 DAXWLF010000000
VERSION     DAXWLF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.138"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(15..545)
                     /locus_tag="ACGE4U_002174"
     CDS             complement(15..545)
                     /locus_tag="ACGE4U_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFF8394292.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(644..994)
                     /locus_tag="ACGE4U_002175"
     CDS             complement(644..994)
                     /locus_tag="ACGE4U_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFF8394293.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1151..2680)
                     /gene="ahpF"
                     /locus_tag="ACGE4U_002176"
     CDS             complement(1151..2680)
                     /gene="ahpF"
                     /locus_tag="ACGE4U_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFF8394294.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2690..3253)
                     /gene="ahpC"
                     /locus_tag="ACGE4U_002177"
     CDS             complement(2690..3253)
                     /gene="ahpC"
                     /locus_tag="ACGE4U_002177"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFF8394295.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3397..4059)
                     /locus_tag="ACGE4U_002178"
     CDS             complement(3397..4059)
                     /locus_tag="ACGE4U_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8394296.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4243..5889
                     /locus_tag="ACGE4U_002179"
     CDS             4243..5889
                     /locus_tag="ACGE4U_002179"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFF8394297.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5880..6962
                     /gene="uxuA"
                     /locus_tag="ACGE4U_002180"
     CDS             5880..6962
                     /gene="uxuA"
                     /locus_tag="ACGE4U_002180"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFF8394298.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         2246 a         1328 c         1299 g         2140 t
ORIGIN      
        1 ataactaaaa atgattaacg tatagtcatt tttcttattc tgatgaaatt gataatctcc
       61 tggatgccca tgaaaatcaa aataaagccg aatatttgga atagaagcat caaacttttg
      121 aatggattga acaataagac gaatccgcca atcaacaaca gaatactact ataaacactc
      181 caacttttag cttttgtatt catgctgaag aacaactgga acaagctgtt gatgataaca
      241 gctaatccaa gaagaatcgg caggatagaa acgattgcag gtgcaaaagc atagacgatc
      301 agagctaaga tcaggaaaaa aagaccactg aacagaccga tactccaaga acctgttttc
      361 cttttaaaac ggtaagcctc aaaaagattc aacaacccaa gcaaaacaag ataggcggtg
      421 atcagataac cgataaaatg aaaaacagca gaagggttta gcacaatcgc aatcccagaa
      481 ataatataaa cagcagctct taataaggcg tatttttgaa tattttgttt aatcgtctca
      541 aacatataag aaaacctcct ttgacttatt ttaacgtgaa ctagtcattt taccaaatta
      601 aatgttacaa aatgagcgaa ggagaagaat atctctggaa aaatcaactt ctgaggatct
      661 tttccatttt tttgcctttt gctagctcat cgatcatttt atctaaataa cgaatttctt
      721 tcatcaagtt atcctcgatg tcttctactc gtacaccaca aacaacacct gtaatcaagg
      781 aacgtgaagg attggggaca ggggcttccg caaaaaattg ttcgaaagtc acttgttttt
      841 gtagttgata ttctagttct gtttgggaat aacccgtcag ccaacaaatg atttcatcta
      901 cttcctcttt tatacgatct tttctctccg cttttgctac atacatagga taaacgcttg
      961 caaaacttgt cgtaaaaata cgattttgcg ccataatcag tcacctttct ttttttcaaa
     1021 ttatttacta cttatagtat ataagaaaaa gagaagaaaa agcgattatt caaaaaaaca
     1081 aaaaggattg tgacaaaatt cttgtcacca tccttttttc cgctctctga ctttttacaa
     1141 tcttcatagc tcaagtgcgc atcagataat cgaatgctcc taaagctgca gtagctcctg
     1201 aacccattga aacaatgatt tgtttgtatg gactatctgt acaatcacca gctgcgaaaa
     1261 cgccggggat attcgttgcg ccatttttct cggtcactat ttcatttctt ggattcattt
     1321 cgatcgttcc atctagccaa tccgtattag ggagtaagcc gactagaatg aatactcctg
     1381 aaacagctag tgtatgcatc tccatcgtac ggcgatcgat ataggagaga gattctacct
     1441 gattcgtacc gctgatttct ttcgtcgctg catgtttgat aacagtgaca ttcgcaagag
     1501 agactagttt atcctgtaag atttgatctg cttttaattc tggtaagaat tctagaacat
     1561 atacatgttt tgctaaccca gccaaatcga ttgctgcttc aacaccagaa ttaccaccac
     1621 cgataacagc aatctctttt cctttaaata aagggccgtc acagtgtggg caataagcaa
     1681 ttcctttatt tttaaactct ttttcgcctg gtacattgat agaacgccag tgcgcacctg
     1741 tcgcgatgat ggcggttttt gctttaagtg atataccgtt agctaagcca atattaataa
     1801 aatgttgctc tttatttaag gaaacggcct gttgattcat cataatgtcg atagggtaac
     1861 tacgcacatg actttctact tgctgcatca attttggacc ttctgtgtaa gaggtaccaa
     1921 tcatattttc aatccctagt gtttcaacga cttgtccgcc gaaagaatca gcgacaagtc
     1981 ctgtacggat tcctttacgt gctgcataga tagctgcact actagctgct ggtccaccgc
     2041 caatcactaa tacatcataa ggttcttttt caaacaactc ttcttcaatc aaagggccag
     2101 tgattttttc aacaatttgt tcgatcgtca tgcgtccgct actaaattct tctccattta
     2161 aaaacacagt tggcactgcc atgatttttt tatcatcgat ttctttttgg aacatcccac
     2221 cttcaatcat cgtatgagta atatttggat ttaatacagc taatatattc aatgcctgta
     2281 caacgtcggg gcaattatga caagtcaagc taacataact gtcaaaatgc aatgtttgtg
     2341 ttattttttt gattttaaga ctcaaagatt cactgatttt cggtgcccgt ccactaattt
     2401 gtaacatagc cagtaaaaaa gaggaaaatt catgaccgag ggggatacca gcaaaaacga
     2461 ttccactttc tttgccaact ggattgatag aaaaactagg tgtgcgcatc gaatctcttt
     2521 tttcccaaga gattttagga gacatttctg ctatttcctt cgtaaattca gctagttttt
     2581 gactattttc actattatcc aaactaacag aaaaaacaat aggagattca atcagtgcta
     2641 aatattgttc gagattttgt ttagtttctt tgtctaacat atccaccacc taaatctttc
     2701 caacaagatc aaaacttggt ttcaatgttt cgccactttc tttccatttt gccggacaaa
     2761 cttcacctgg gtgggtacgt acatattgag cagcgcgaat cttatcaatc agtgcactag
     2821 cgtcacgacc gattccatct gcattgattt ccatagcttg aacgattccg tctggatcaa
     2881 taataaatgt tcctcgctga gctaatcctt gctcttcatc caaaacatca aaaatacgag
     2941 aaatctgatg agacggatca ccgatcatca cgtattcgat tttaccgata gcatctgatg
     3001 tatcatgcca tgctttgtga gtaaagtggg tatcggtaga gcaagaaaag acttcaacac
     3061 ctaaagattt caatgtactg tattgttctt gtaagtcttc taattcagta ggacaaacaa
     3121 aagtaaaatc agctggatag aagcaaagaa tactccagtt ccccataaga tctttttcac
     3181 tgacttctaa aaattctcct tgatgataag catgtgcatt aaattcttcg atttttttcc
     3241 caatcaatga catagtaaaa cctccaataa taattattta tttataatta ttgtaattta
     3301 gttggagttg caaagtcaat ggatattttg ttatttaata aagattactc aaaaacgaag
     3361 gttaattata aaaatagaag aggtcgcaag aaaatgctac tgctggaaat actcgggata
     3421 acgttgagta acagtttcct gttctttcag catcatgtgt aagtgcaatc ccgtttgaaa
     3481 tcgagcttct tctggttttt tatcaactaa agccttgagt aaatcataat gttgttccat
     3541 gatcgtttcc catggaagcc cctgaatggt cactcgtaac cagcggaaac gttctaaatg
     3601 ggtattatgg tcatctaacc agttccaaac ttcttttctt cctgcaattt cgaaacaagc
     3661 ttcatgaaat aaattatctg cacggaaaaa agcgcgttcg tcatttattt tgactgcttt
     3721 ttcttgttct tctaaaatcg tttctagcac tattcgtgtt tgtgtgtcca atttggaaca
     3781 acattcaatc atgatttgct cttccaactg ttctcggaca aagcgagcat tttctgcaga
     3841 aacaagatta atttttgaaa catacgtacc actttgaggt atcgtataca cgagttcttg
     3901 ctgacgtagt tgaatcaatg cttcgcgaat gggagtccgg ccgatagcaa gatgttcctc
     3961 cagtgccttt tcagatattt tccttccagg ttctaagtca gcatagataa tttgtttacg
     4021 aatcgcttga taagcttgag attgcaagct gcttttcatc tttattcctc caaaatcatt
     4081 tctatgaacg tagtataaca gaagaggtct gaaaaacaat ctggtatgtc acattaacta
     4141 gtaaatttgt ataaatagta aaattttggt tgacatagcg atttttattg cataaaattc
     4201 acttgaatac aaaaactaat atgttagttt aggaggggaa aaatgattga tttaaacaac
     4261 tggaggaact ttatccctga attatctcaa aaagaagtag aaatacctca ttttgaccgt
     4321 gcatcattaa gagaaaatgg acaaaaaaat cctgtctgga tccatttcgg tggcggaaat
     4381 ctttatcgag gatttcatgc agaaatcgct caacgtttag cggatctagg cgaattgtcc
     4441 actggtgtca ttgtttgtga aacattcgat gaagaaataa ttgaaagggc ttaccaagat
     4501 tttgatgatt tgattcttga agtggtcatg ccaagcagtg gagagttcgt caaacgtata
     4561 ttagctgcga cagctgacag tctgtattgt catccagatt gttctgaaca ctttgataag
     4621 atgattcgac actttgaaaa tcctagttta caattagtta cttttgctat tactgaaaaa
     4681 ggttatcgat tgaaagatac aaataataaa tatataggac cgattgcagt cgatataaaa
     4741 aatggtccaa agaagccatc acatacaatg agtattcttg ctgcgctact ttataaacgt
     4801 tttttatctg gagcttggcc gattgcactt gtctctacag ataatttttc acaaaacggt
     4861 agtcaattac gtacttcttt gctaacgatc gcaaaagaat ggcaaaagga aggttatgta
     4921 caaaatacat ttatccaata cttatcgaat gaagaaattg ttgcgtttcc atggacgatg
     4981 attgatcgta tcaccccgaa tccttccttg tcagttagca aaatgttggc aaaagagggc
     5041 ttttcaaata tgtcgatttt ccaaaccgca aaaggaaata atattgctcc atttgtcaat
     5101 acagaggaca tccattattt aatcatggaa gatcattttc ccaatggacg tccgaaattc
     5161 gaaaaagctg gagtattctt gactgatcgt gcaacggtag ataaagcaga tacgatgaag
     5221 gtgacgactt gccttaatcc tttgcatacg tctttagcta ttatgggttg tttactgggg
     5281 tataggagca tagctgagga gatgaaagat ccagatttat cgctgctagt ttctgaaatc
     5341 gggtacaatg aaggcctccc agttgtggag tctccaggta tcattgatcc aaagatcttt
     5401 ctggaagaag taattactgt gcgcttttct aatccaaata ttcctgatat gcctcagcga
     5461 atcgccacgg atacctctca aaaggtggcg attcgttatg gtgagacgat taaaaaatat
     5521 atggaaatag aagttaaaga cccgcagaca cttgtattca taccattgac tctcgctgct
     5581 tggcttcgat accttattgg cattgatgat caaggaaaac cattcatccc gagtccagac
     5641 ccactattga cagactttca gcataagctt tccgatattc aactgggtac gcaagataca
     5701 tcacatattc atgagaaaat ggctcctatt ttgtcaaata aagaaatttt tggtgtagat
     5761 ctttaccaag cagagcttgg cagaaaaata gaagaaatgt tcatcaagat ggtagcagga
     5821 ccgaatgccg tacgaaaaac attacaggat tacttacaag aacatggagg aaatttatca
     5881 tggaaatgac ttttagatgg tacggatcac aggaagaaaa gattacctta gatgaaatca
     5941 gacagattcc tgcggtcaaa gggattgtag gcacattgat ggatatacca gtaggagagg
     6001 tatggcctag ggagaggatc aaagaactaa aagaagaaat tgagtctcgt gggttaacac
     6061 taaaagtaat tgaaagcgtc aatgtgcatg atgccattaa aattggatta ccttcccgtg
     6121 agcagtatat cgaaaactat aagcagacaa tccgaaactt ggcagaatat ggaatagaag
     6181 tcatctgcta taattttatg cctgtatttg actgggtgaa atctgacctt gactaccgct
     6241 tagaagacgg ctcatccacg ttagctttta tatctgatga tattccagat gatccaagag
     6301 aaatcgtcgc gcgcatcgaa cattccagca atgaatttga attgccggga tgggaacctg
     6361 agcgcttagc tcatatcaaa tccttgcttg aagcctatgc atcagtagac gaagaaaaac
     6421 ttcgagaaaa ttttgcctat tttctccaat caatcatccc aacatgtgaa gaggtagggg
     6481 tcaaaatggc tgtacatcca gatgatccgc catactcgat ttttggatta cctcgaatcg
     6541 taaagaatcg agaagattta gattggttgt gccgcatagt agacagccct tataacggaa
     6601 tcactctttg tacagggagt attgcagaag atccagaaaa tgatgtatat gggattttag
     6661 cagaattttg tcaaagagat cgaatcccat ttgcacatgt tcgaaatatt aaatttttag
     6721 ctaatcgaga tttctatgag gcaccgcata aatccgaata cggttcatta gacatgtacc
     6781 gcatactaaa aacaatgtat gacaacggat ttaacggata catccgtcct gatcatggaa
     6841 gaatgatctg gaatgagaca ggaagaccag gatacggctt atatgatcga gcattaggtg
     6901 ctgtttatct aaatggatta tgggaagcaa tcgaaaaagg aaacaaaagt aaagataatt
     6961 agtgaaaaaa actgtaattt tattatgtaa ataagaagga tgttcaaatt ctt
//
LOCUS       DAXWLF010000137         6808 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXWLF010000137 DAXWLF010000000
VERSION     DAXWLF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.139"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(180..758)
                     /gene="pgsA"
                     /locus_tag="ACGE4U_002181"
     CDS             complement(180..758)
                     /gene="pgsA"
                     /locus_tag="ACGE4U_002181"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFF8394299.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(967..1890)
                     /locus_tag="ACGE4U_002182"
     CDS             complement(967..1890)
                     /locus_tag="ACGE4U_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFF8394300.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(2629..3915)
                     /locus_tag="ACGE4U_002183"
     CDS             complement(2629..3915)
                     /locus_tag="ACGE4U_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFF8394301.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(3908..5176)
                     /locus_tag="ACGE4U_002184"
     CDS             complement(3908..5176)
                     /locus_tag="ACGE4U_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFF8394302.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            5428..5889
                     /gene="mscL"
                     /locus_tag="ACGE4U_002185"
     CDS             5428..5889
                     /gene="mscL"
                     /locus_tag="ACGE4U_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFF8394303.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(5948..6394)
                     /locus_tag="ACGE4U_002186"
     CDS             complement(5948..6394)
                     /locus_tag="ACGE4U_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8394304.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(6487..6732)
                     /locus_tag="ACGE4U_002187"
     CDS             complement(6487..6732)
                     /locus_tag="ACGE4U_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFF8394305.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         1928 a         1444 c         1205 g         2231 t
ORIGIN      
        1 ggcaaggagc tgtgacaaaa atcttgtcac agctccttgt tccaaataaa cagtgttctc
       61 ctgccaaaac ctcggtcata agtcacaata tacataaaac atgaaaagct gttttttgaa
      121 attatccctt gtgactcaga gttagacggc aggctcacaa cctctccact aaacgatact
      181 tacattgatc ctttgaacac accgctgttt ttgatgaagt aatccacacc tgaatagata
      241 gtaaagatca gacaagcata aagcatgatt tgatctactg gaataccgat caggctgaat
      301 gggatattat tgataaacag caagataata gcaaccattt gtgtagccgt cttgactttt
      361 cccggccaag ctgcagccat gacttctcca tgttcgacaa gtagtaaacg caagcctgtg
      421 acggctaatt cacgacagac aatgatcgct actacccaac caggggcctt tccttggtca
      481 accaacatga taaaagcagt cataactagc attttatctg ccaacgggtc cgcaaacttg
      541 ccaaaattag tgacaaggcc tcgcgcacgg gcaatttttc catctaacca gtctgtgata
      601 ctagctaaag caaaaataac tgcacctact agctgtgtga cagccagtgt ggtgtctcct
      661 acgttaaatt gtccccaatc taaaggtaaa cctacaacca aaatgaaaat cgggatcata
      721 caaattctaa gtacagttaa tttatttggt aaattcacac tatcgactcc tttcttccct
      781 attctacgtg actatcagcg aagtttccac cgatattatt tacttttcca aatgaattta
      841 ttcaaaggag tatttttatt gaaattgatc tttttgaatt aacgaagaag gaccgcgaca
      901 cagatttgtc acgatccttc ttttttatac ctacaagtaa acagatgcgt aaactaattt
      961 atacgatcat tctgcgtatt gaagagtaag gttcacattt tttggactct gattgttttc
     1021 ccctggatta actggtacgg attctccatt cgctttgatc gttgcattgc ttgctgcacc
     1081 aatcgtgatg gtcgcatttg ttgctgtttc cggtaacacg gtactctgtg tttcatttgc
     1141 tgccagtgtc ccttgataaa cataggcatt gtctactagc acacctaacc atacacgatt
     1201 gacagcagta aattcaaggg ttacaggctt ggtagcgtct gtgatattta ctgcgatggc
     1261 actgccagaa tcttctcctg tagctattgc cattttcttt tcttcacttg attttgttgt
     1321 tgattctact gtagaagact ctttggtctg cgtgctttgc tctgaggaag aggactcctc
     1381 gactgatcct tctactttaa gcgaagaagc tgtgtctccg attattggat cagcttgacg
     1441 atcttgccaa gtcatatagc ccacgacaac gacaatcgcc aaagcaacca atcctaataa
     1501 aatgacagga agacttgtcc aaaagcgact tggatgcgat tcttcctgat gcattgcttt
     1561 tctagaagta cttactgttt caggctgcgg acgttcaggg accgatcctg tcaatttgct
     1621 tttcccatca aaaacatcga ctaaatggtc tccatcttct cctacagctt cagcgtattg
     1681 ccgaataaat gcccgcacgt aaaatttacc aggcaattgg tcaaaatcat cattgtcgat
     1741 tgcttccaaa taacgttttt gaatttttgt aatctgctgt aattcatcta aagatatttt
     1801 tttattcagg cgtgcttgcc gtaatttttc tccgatggtt tcaaaagcca ctcgttcatc
     1861 ttctccagtc tgtttgattc tccgaagcat ctgctgttgc aacgaaagac gttatgtttg
     1921 ggtgctgtga caaaagtcat gtcacagctc cttttttccg aataaacatt gttctcctgc
     1981 caactcctcg gtcacaagtc acaatatcca ataaatataa gaagctgttt tttgaacttg
     2041 ttccttgtga ctcggagtta aacggcaggc tcacgacctc tttcttaaac ggtgttcaat
     2101 cagctgttcc tcggtattag ctcaaaaacc ggcaaaatat aaggagatat ttttccgtat
     2161 gtttctctaa tactcaggtc aaaacgctga tttcacaacc tcttccttaa acggtgttca
     2221 aaagcttgca tctgcggtaa taagtccaac caagttaaaa acattaagaa gctgttttta
     2281 cttggctgtt cttattactt gatgcagggc agctttttca caacctcttg gtacacggtg
     2341 ttccagaagt agcttcttcg gcaataagcc gaaatatcac aaaaatttga gaagcaattt
     2401 tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc tttgattact
     2461 tcgtttgatt ttattttaat ataagttgat taaaagagag catgatttgt tttattttct
     2521 cactaatttt tccaacacaa ctaagaatac cataaaagct gattttggag gaggttccta
     2581 ctacaagtct aaaacgaaat ttaagacttt tcctcataaa atactggttt attttggtct
     2641 catgtaaaac tcgctgaagg cctcttcact tataaatgct tccccagctg ccagtacgtc
     2701 tttcatttgg atcgaatcga tgatttcagg aagatcaaat aaagttcgat cgccaaataa
     2761 actttgcgta aattgattgg caatatattc gatcgaattt aatgattgga aatattgtcc
     2821 aagcattttc ttcttcagca aatcgagatt tgtttctgac acttccggat catcagcgaa
     2881 tccaagaatg atttctttga ctttttctgc tgctttctct ggttcgtccg tatctccgct
     2941 gaagtccgca aagtgaaatt cacgatccaa gctgaattca aaaccaaagc tgtcatcgat
     3001 aattccttga ttgtacatcg ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa
     3061 caacagattg attgctgttt tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc
     3121 taatccttta ataccaagta caaatttatc tcgtgtaatg gctgcttcca atgcactttg
     3181 tttgatgatt tcttttgtgt tttcagggaa ataacgtaca atttcttgct ttggagggaa
     3241 attttttgct tcttggtttt cccgaataag tttcatcaat ttctcaggct ccatcttacc
     3301 aactacaaat aaaaccatat tgcttggctg ataaaacgtc cgatagcacg tatacaagtc
     3361 ttgagctgtg attttgtcga tactttcaac cgtgcccgca atatcgatat gcaatgggtg
     3421 tgtaggatac aagttattta gtatgccaaa aaacatccgc cagttagggt cgtcttcata
     3481 catttggatt tcctgaccga tgattccttt ttccttgttt acagtttctt ctgtgaagta
     3541 aggtgcttgt acaaaatcga tcagagtcgt cagattcttt tctacctgat ccgtcgtaga
     3601 aaacagatag ctggttttcg taaaacttgt gaaagcattg gcggaagctc cttgtttccc
     3661 aaataattga aagacatcac catcttcttt ttcaaacaac ttatgttcta aaaaatgggc
     3721 aattccatct ggaactttca ccttttcttt ttcaccatag ggaataaatt cgttatcgat
     3781 cgaaccataa ttcgtactga acaaaccata tgtcttgtga tagtcatttt taggcaacaa
     3841 atacacggtc aaaccgtttg gcagtacttc atgataaagt gtttcattga tttgctcata
     3901 ttctgtttta ttcatttgca tttcctcctg ctaaaaagaa gattgcctgc aattcgattt
     3961 gctcagctac ttgttgtatc tctttgatcg ttacgctctc catacgtttc aaccattctt
     4021 catcagataa ttttgtttcc ggcagccagc tgtccaaaaa agaagcttcg attgccgctt
     4081 gcggagagtc tagagaaagc agatattggt ttctcagcat tgcttttgtc tgagacagtt
     4141 ccaactctgt tatttcacca tttcgcaaac tttctaattg ctcgtgaatc agatgaagta
     4201 cttgatttcg gttcttttca tcgataccgg tttggacact catgaatcct cggaacgtat
     4261 ccacgctact agaggcataa taagctagac tttctttctc tctgacattc atgaaaagtt
     4321 tagagtgagg aaaaccgcca aataaaccgt taaagaccat tagcgcaaaa cgttctggtt
     4381 catcgtaata cacatttgtc tgataagcta ggttcagctt tgcttgaacg attggctcac
     4441 gtacttgtcg ttccttcacg atattcactt gaggttgggt ataaaacaga tctggataaa
     4501 ctgtttcccg cgactcaaat ggtaattgcc caactgcttc agctgctttg tccggatcaa
     4561 catcgccgac aacaaaaatg tcgatttgat catttgccat catttcttga tagtaggacg
     4621 ctagcgaact agcagtgatc ttgtccaaat cttcgactac gcccaaccct gggatctttt
     4681 gttccgggct gttttcaaaa taaagttctt gcaaggcata ggaagcaaaa gtttgtttat
     4741 cttccttcaa actttccaaa tatagacgca gattgttctt ttccaagtca aacgtcaact
     4801 gatcaaactg atgattttta atatttggat aaaataaaac ctcttttaaa aaatctaccg
     4861 cttgagaaaa aaggtttgga tcattgacat attcaccatt cacaaaactg atccctgcgt
     4921 ttagccaatg taaatttcct ttttttctaa ctgataatcc aaagcttgcg ccataaagct
     4981 ctgcaagttg cgcacttaac tttgtctgat ccggataatt caaactattt gtttccaaca
     5041 tgctgctgag aagtgaacgt ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga
     5101 taaaaatccg gatcgttttg tattgttttg ttggtaagac atgaagattc acgccttctg
     5161 ccaattgata caccatattt atatgctcct tttcacagtt gattgcccgc ttactctttt
     5221 aatgggtcaa cctgcctttt ttacttattc ctctgcttga aatttataag ttcaggcaat
     5281 tcgccgtttt ttctaaatga tgttacactt tatagtatag catagatgtt ttttatcaaa
     5341 agaaacagac ggctaaaagc gtatattgtt tttttatttt ttatttttcg atatactaaa
     5401 gacaaataaa agaagggagg gtcattcatg atcaaagaat tcaaagagtt catcatgcgt
     5461 ggagatgtct tggatcttgc tgtcggcgtt gttattggta gtgcgtttac aagcatcgta
     5521 aaccaagtgg tgcaaggctt gatcacacca ctaataggtt ggtttgtcgc aatgatcaca
     5581 ggtacttctg atttagaagg agcactttca attcttgact ggtcgccgac aaaaggcgtc
     5641 acatttgcgt ttggtaacgt tattagtgct atcattacat ttctgatcac tggttttgtc
     5701 ttattcttag ttgtcaaagc ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa
     5761 gtggaagtgg aagaaactcc tactgcagaa gattatctta gtgacatacg cgatcttctt
     5821 gccaaacaag tggcagaaga caatacacat ccagtagagc aaacaaatct ttccgatact
     5881 tctaaataat aaagaatcta aaaaaaagaa agccgatttt cagtgtcggc tttctttttt
     5941 tagattctta acttgctact tgttcctttt tcatgtcgat atgtggaata tcatcttcca
     6001 aatacacatc tgagattgcc ttgaatccaa acgactcata aaaagcttgt aggtattctt
     6061 gtgcgccgat cacgattggt ttttctggaa aatttttctg gatcatgtta atcgtttctt
     6121 gaacaatctt tcttcctaaa ccatctttac ggttgtcctg atgaacaagg acacgtccaa
     6181 aatggatact ctccgcttct tcatatactc gcgtgtaggc aaggatcttc ccatcagtat
     6241 cttgaaaaaa cgtatgccaa gcttgtccat caatgtcatc aacttcttga taaggacact
     6301 gttgttccac aacaaaaact gcgattcgtt ctttgacgat cgcgtaaaac tctgctgttg
     6361 acaattcttc aaatttcttt acctgatatt ccactaagca aacgccccct gcttttttat
     6421 tcttagcata ccgattcgca cacaaaaaag caaacgaacc ttacgaatct caggctcgtt
     6481 tgctttttaa actgcttcag ttaaaaattc gatctcaact gtccgagtaa gtacatctga
     6541 atagctgtaa gatactcgtt caaaagaatt ttcttcagga tccaaatcaa caacgaatac
     6601 tgaaggatag gtttctgtta aaacgccgcg gcgttctgtt tgacgctttc ttcctgtctg
     6661 agcaaccaac atgattgggc tgccgatacg accttctaaa tctttcttga ttgaagctaa
     6721 agttgttggc atacagttca cctctactat caactatacc acatattttt aaaattttca
     6781 agtttaccat aaaaaaaaaa gaaatgca
//
LOCUS       DAXWLF010000138         6789 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXWLF010000138 DAXWLF010000000
VERSION     DAXWLF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.140"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            344..613
                     /gene="rpsN"
                     /locus_tag="ACGE4U_002188"
     CDS             344..613
                     /gene="rpsN"
                     /locus_tag="ACGE4U_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFF8394306.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            628..807
                     /gene="rpmF"
                     /locus_tag="ACGE4U_002189"
     CDS             628..807
                     /gene="rpmF"
                     /locus_tag="ACGE4U_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFF8394307.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            839..1216
                     /locus_tag="ACGE4U_002190"
     CDS             839..1216
                     /locus_tag="ACGE4U_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394308.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1237..1386
                     /gene="rpmG"
                     /locus_tag="ACGE4U_002191"
     CDS             1237..1386
                     /gene="rpmG"
                     /locus_tag="ACGE4U_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8394309.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1410..2393
                     /locus_tag="ACGE4U_002192"
     CDS             1410..2393
                     /locus_tag="ACGE4U_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFF8394310.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHREERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKHYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2423..2545
                     /locus_tag="ACGE4U_002193"
     CDS             2423..2545
                     /locus_tag="ACGE4U_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFF8394311.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2563..4086
                     /locus_tag="ACGE4U_002194"
     CDS             2563..4086
                     /locus_tag="ACGE4U_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFF8394312.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4110..4349
                     /locus_tag="ACGE4U_002195"
     CDS             4110..4349
                     /locus_tag="ACGE4U_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394313.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSV
                     DTVHRALKKDPDCCFIKVNTGRSRRLAKRYNILIN"
     gene            4832..6382
                     /locus_tag="ACGE4U_002196"
     CDS             4832..6382
                     /locus_tag="ACGE4U_002196"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8394314.1"
                     /translation="MSDKNTYACRSQRHKQESDTYENPDQLNETVTSKTANENKQSRI
                     PKKNSNYSKYIFALFTALILVTVATAGYIVYKLKQQEAEAKAKYETAAKNLMASIQEE
                     QDQSRISTKIDTISDGENKCLVYRPVDESAVPFKNANQLLDELAQKQQKKHCEKEVLT
                     VARIKATAISSKVGQYSIEADSFIWDRSKEHFKNPDNISEKAIYVSEKTGKEITNKDL
                     IPDEGSLLGIQQVIQQSILDHAEKPEKIIDAVLTMDRISYDSKMTYTPDELTIILPNN
                     SIGTSKVTLNYKDIAPFIDTDLVSQESIKDALPALDENKKYVALTFDDGPNNSSTLDL
                     LNILKTDNVKATFFMLGQMVDQNPDVAKQAHDEGHEVACHSYSHPQLNALSTDELRSE
                     INEANKAIFKATGVLPRNIRPPYGAIDKKSAETIGMPIIQWDIDSQDWKSKNPRTINN
                     VVKQNVYSGAIILIHDIHPESVKAIPNMISMLKNEGYEFVTIDQLLSGKQKTLHQYFG
                     MNDERLVD"
BASE COUNT         2588 a          983 c         1275 g         1943 t
ORIGIN      
        1 gggataaacc agttaaacat aaaagattga ataaaaagtt atagtttctt tcatattgcc
       61 gttttaagta gaggaaaagc tacttaaaac ggtatttgtg tttaattgga ctaataaaag
      121 tgaattctta tttttcagag cttaaactgc atattctttt aaatgatttg attatctcta
      181 tgtaccgata aattcgtttt caaatagtta tctattcata tgagttttat ttcatgttat
      241 ttattgttga caagagcaac tcttgttggt aaagtatcag tgtcaaaacg taacgattac
      301 gatttgagat gtctaaatca aaataaaagg agtataaagg tatatggcaa aaaagtcgaa
      361 aattgctaaa gcaaagaaac aaatggcgat gattgaaaag tacgcagata aacgtcagga
      421 attaaaagcc gcaggagatc gcaccgctct cgctaaattg ccaagagatt caaatccgaa
      481 tcgtctgagg cttcgtgatc agacggatgg tcgtccaaga gggtatatgc gaaagttcgg
      541 tatgtcccgt attaagttta gagaacttgc ccatcaaggg ttgattcctg gagtaaaaaa
      601 agcaagttgg taaaaggagg aaatatcatg gcagtaccag caagaaaaac atctaaggcg
      661 aagaaacggt taagaagaac tcatcaaact gtagtaaaac cagaaatttc atttgacgaa
      721 gcgaatggtg attatagacg tagccatcat gtatctttaa aagggtacta taatggaaag
      781 aaagtaatta aaggagcttc aaaatagttt aatatataac ttttaggagg gaatcaaaat
      841 ggaaattatt tacccacctt tagttgaaga aggattaaag tattatcttg aaacgaccca
      901 gcaatcgcta gataaatgca ctttttatcg ttcgatggta gagagaggaa ttattactga
      961 aacgggttta ccgacccaac aggcaattga aaatggatta gtcaaagatt actatgagga
     1021 ccaaggctta tcatttgatg attttttaag aatctatcca atttttgaag agtatgacga
     1081 ggagttattt caatgtattg atggttattg ggagatacct gttgatatga aagaaaattt
     1141 agtttcacaa ttggaatctg gcgagttaac ttttgaagat gcacaacaaa ttcaagcata
     1201 tcttgaagat cgataagtat tgaaggagga agatttatgc gtcaaaatat tattttggaa
     1261 tgtgtcgaaa ctggagaacg tctgtatctc accagcaaaa ataaacggaa taaccctgaa
     1321 cggttagaat taaaaaagta ttcgccaaaa ttgcgcagac gggcaatttt caaagaagtg
     1381 aaatagctaa gtggtaaaaa ggtgacaaaa tgggcatacc agttacaatc gtttctgggt
     1441 ttttaggggc cggaaaaaca acgctaatca atcaagcctt gcaagtctca ccctacccca
     1501 gggaagaaat tattataatt gaaaatgagt ttggtgaagt tgggattgat cacaaattat
     1561 tagttcatag agaggaacgg gtgttacaac taaataatgg atgtatgtgt tgcagtcttc
     1621 gcagtgattt attagcaata ttgatttctt tactggaaac aatagaagaa catcaacaac
     1681 caatttcaca aatcatcatt gaaactacag gtatagcaga tccccaacca atcattcaaa
     1741 cacttttaac agcgcctcaa ctaagaggaa gagtatatat cgatagttta ttgactgtta
     1801 ttgatagtga cagttttgaa cttattgaaa gagaaccaca agcccttaag caacttgtca
     1861 tggcagatcg catttttgtg tctagaaaac aaaacttgaa tttatctgca ctaaaaaaat
     1921 tacagagaga aatcaaaagt attaaccctt tatccgatat tcgaatgttc acgcatagtg
     1981 aaacgattca aaaaaaggat ttttataatt tgaaaaaatt taatcatccc ttagtttttg
     2041 aaagtgattc atcagaaaat cactataatg gcaaacacta tcatcaacac catcaagtac
     2101 atgagtttaa atcaattctc ttaacttcag atcaagatat aaaaaaagat tttttactcc
     2161 aatggattga ctggctgatt ttaaccaacc aagggagcct gtatcgtttt aaaggattga
     2221 ttgctttaca agagcaggat tttatagttg ccatgcaagg ggtaaacgaa caggttaatt
     2281 ttcaatacac cactcgacaa aaagaccaaa ccactattat tctaatcgga aaaatgttgg
     2341 atgaagaaag gatcaaacag agttttgata aattactggc ggaaagcaaa taatgataag
     2401 tattgattgg gggtaatttc taatggagaa aacggatata tctagtgcat atcgacgact
     2461 taaaagcccg aatattaaaa ctagaaaaag ggcattaaaa attatcaaag aacataaacg
     2521 aaataaaatg aagaaattag cataattaag gaaggaaaaa gaatgaagaa aatcagtgta
     2581 ggacttattg ggatagcagc tctaggatta ttgggagctt gctccagcac taatgatgca
     2641 aaagtatcga atgataaaga tggaaaatta gaaattgtaa caacattcta tccaatgtat
     2701 gatttcacca aaaacatcgt cggcgatgaa gcgaacgtgg atttaatggt tccagcggga
     2761 tcagaacctc atgactatga gccctcagct aaggatatgg caaaagctca tgatgctgat
     2821 gtttttgtat atcataacga aaacatggaa tcatgggtac caaaagctaa agaaagttgg
     2881 aagaaagctg ggcctaatgt agtagaagga acaaaagaca tgattttatt gccaggtagt
     2941 gaagaagaag atcatgatca cggagaagaa gatcaccatc atgaactaga tcctcatacg
     3001 tgggtttcac ccaaaatggc aattaaggaa gtttcaaata tcaaggatca gttagttaaa
     3061 ttgtatccga aaaaagcaaa agtatttgag acaaatgctg aaaagtattt gactaaactg
     3121 aaacggttgg atgctgacta taccactagt cttaaagaag cgaaacaaaa aagttttgtg
     3181 acacaacatg cagcctttgg atatttagct ttggattatg gattgataca agttcctatt
     3241 gctggactaa gtcctgaaga agagccatct tctggtcgat tagctgaatt aaaagagtat
     3301 gttaaaaaaa ataagatcaa ttatatttac tttgaaaaga atgctaatga taaaatcgca
     3361 aaaactttgg ccaatgaagc tgggataaaa ttggaagtat tgaatcctct tgaaagtctt
     3421 acaaaagaac aaatggataa tggtgaagat tatgtttcag taatggaaga caatctaaaa
     3481 gcgttagaga aaacaactat ggttgctggt gaagaggtag ttccagaaaa agaagcgaag
     3541 gatgaaaaga cagttgctaa tgggtacttt aaagatgccg atgtgaagga tcgcgaattg
     3601 agtgattata caggcgaatg gcaatctgtt tatccgttgt taaaagatgg aattttggat
     3661 gaagttttcg attacaaagc aaaactcaat aaagacatga cagctgccga gtacaaagat
     3721 tattatacta ctggctataa gacagatatt gatactatca acattaaaga caacaccatt
     3781 gattttgtag tcaacggtga acatcatcaa tacacataca aatatgttgg atacaaaatt
     3841 ttgaattatg aaaaaggaaa ccgtggtgtt cgttttaatt ttgaaactga tgatgcaggt
     3901 gctggtcgtt ttaaatatat tcaatttagc gatcatggaa ttgcaccaag taaggcagag
     3961 catttccata ttttctttgg tggagaaagt caagaaaaac tttataatga aatgcataat
     4021 tggcctactt tctatccagc ttcattatct gagcatgaga ttgctcaaga aatgatggcc
     4081 cattaaaatc caatctttgg aggaaataca tggtaagaaa agtattattg tctatggatt
     4141 ccaagaatat tgatgcttat cggcgttatc gatttgcaaa gcaaatgaga ttaaatggga
     4201 gtacattagc tgatattgct gcaactttac atgttagtgt tgacacagtt catagagcat
     4261 taaaaaaaga tccagattgt tgttttatca aggttaatac tggaagaagt agacgtttag
     4321 caaaacgata taatatcttg ataaactaat tgtaacacta aagcagttca attcttcctt
     4381 aatggaattt gatctgcttt ttattatgca tagaaagagg tttcctatga aaaatatgtc
     4441 acaattctac tagttatttt gatttaagaa aaacaacact tttaaatcat atagaagaga
     4501 taattagcag taaaaatgaa gaataggtaa tattagaatg ggattcaact aacatattta
     4561 aaaacatgac aaaaatgtaa ggatattatt tatgataact tattttctga taccgaaaat
     4621 gatgctatta tttttttaga cattcagcta aaaaataaga gaggaagaag aaatatgtaa
     4681 aaaaaccttt taaattggtg tacgtataaa atgctgagaa aacatctata attactacgg
     4741 caaaagtaaa aaaatagtga aaaatgccag gtaaaaaata agaaatttct atgttttatt
     4801 tatatgatga taaatggagg aataaggcca tatgtctgac aaaaatacct atgcatgtcg
     4861 gagccaacgt cacaaacaag aatctgatac ctatgaaaat ccagatcaat tgaatgaaac
     4921 tgtaacgtcc aaaaccgcaa atgaaaacaa acaatcaaga ataccgaaga aaaattcgaa
     4981 ttatagcaag tatatattcg cattgtttac cgcacttatt ctagtaactg ttgctactgc
     5041 tggctatatt gtctataaat taaaacagca agaagcagaa gctaaagcaa aatatgaaac
     5101 tgctgcaaaa aatctcatgg cttcaatcca agaagagcaa gaccaaagta gaatttcgac
     5161 gaaaatagat actataagtg acggagaaaa taagtgcctt gtttatcgtc cagttgatga
     5221 aagtgctgtt ccttttaaaa atgcaaatca actcttagat gagcttgctc aaaagcaaca
     5281 aaagaaacat tgtgaaaaag aagtccttac tgttgccaga ataaaagcaa ccgcaatctc
     5341 ttctaaagtt ggtcagtata gcattgaagc agatagtttt atctgggatc gtagtaagga
     5401 acattttaaa aatccagaca atatttctga gaaagccatt tatgtttccg aaaaaactgg
     5461 caaagaaatt acaaataagg atttgattcc agatgaagga agtctcttag gtattcaaca
     5521 ggtgattcag caatccatac ttgatcatgc cgaaaaacct gaaaaaatta ttgatgctgt
     5581 attaacaatg gatagaattt cttatgatag taaaatgact tatacacctg atgaattgac
     5641 tatcatctta ccaaacaatt caattggaac ttctaaagtc actctaaatt ataaagatat
     5701 tgcgccattc attgatacag atctggtttc gcaagaaagt ataaaagatg cattacctgc
     5761 gttagatgaa aataaaaagt acgtcgctct cacttttgat gatggtccaa ataatagctc
     5821 aacacttgat ttgttgaata tactaaaaac agacaacgtg aaggctactt ttttcatgtt
     5881 aggacagatg gtggatcaaa atccagatgt tgcgaaacaa gctcacgacg aaggtcatga
     5941 ggtagcatgt cattcgtatt ctcatccaca attgaatgca ctttcaacag acgaattacg
     6001 aagtgaaata aatgaagcta ataaagcaat tttcaaggca acaggtgtct tgcctcgcaa
     6061 tatccgtccc ccttatggcg ctattgataa aaaaagtgct gaaacaattg gtatgccaat
     6121 tattcaatgg gatattgact cacaagattg gaaatcaaaa aatcctagaa caatcaataa
     6181 tgttgtcaaa cagaatgttt atagtggtgc aatcatcttg atccacgaca tccatcctga
     6241 atcagtcaaa gcgataccaa atatgatctc tatgttaaaa aacgaaggat acgaatttgt
     6301 gacgatcgat caactattgt caggaaaaca aaaaacattg catcaatatt ttggtatgaa
     6361 tgatgagcgg ctagtagact aactgttatt agattgcttg gtatgtgtag aagttatact
     6421 tgcaattctc tctcagaaaa aatgttaagc aaaacaaatt gataaagagt atcgagaatg
     6481 ctaattctta ttagtcaaag ttctatttta gtaaataaaa aaagtactgg aagttttgtg
     6541 atcaaaaatc acatcacaaa attggttgaa aaaacatttc tttttataat gccactcatt
     6601 attgaaacct ttttgaaaaa gtttatacaa gctagatata actttattag tttacagaat
     6661 gcaattaatg cgatgaagat ttattgagtt gatttaattt taacataatt aaactagtta
     6721 ttaatactaa tagtaagtca aaagaaaaat ctattaatgt agtaatactg atcagaatag
     6781 aaagactgg
//
LOCUS       DAXWLF010000139         6776 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXWLF010000139 DAXWLF010000000
VERSION     DAXWLF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.141"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            9..899
                     /locus_tag="ACGE4U_002197"
     CDS             9..899
                     /locus_tag="ACGE4U_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFF8394315.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(972..1796)
                     /gene="nadE"
                     /locus_tag="ACGE4U_002198"
     CDS             complement(972..1796)
                     /gene="nadE"
                     /locus_tag="ACGE4U_002198"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFF8394316.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1808..3280)
                     /locus_tag="ACGE4U_002199"
     CDS             complement(1808..3280)
                     /locus_tag="ACGE4U_002199"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFF8394317.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3917..4279)
                     /locus_tag="ACGE4U_002200"
     CDS             complement(3917..4279)
                     /locus_tag="ACGE4U_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFF8394318.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4502..5254
                     /locus_tag="ACGE4U_002201"
     CDS             4502..5254
                     /locus_tag="ACGE4U_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8394319.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5394..6317)
                     /locus_tag="ACGE4U_002202"
     CDS             complement(5394..6317)
                     /locus_tag="ACGE4U_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8394320.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6332..6604)
                     /locus_tag="ACGE4U_002203"
     CDS             complement(6332..6604)
                     /locus_tag="ACGE4U_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394321.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2068 a         1349 c         1158 g         2201 t
ORIGIN      
        1 tgatatatat gcaactacaa ataaacgaag aatccttacc ggtatatgaa gcattagcta
       61 gtaaaacaag gatcaagatc atccagctac tttccaaaaa aaagatgaat gtcaaagatt
      121 tggcaaaaga acttggagtg agcagtgcta tcacgacgat gcacgtgaaa aagctagagg
      181 aagcaaacat catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc cttcgtgtcg
      241 ataaaatcga tatcagtttt ccagaaaaaa tattcaatgc ttttgatacg aaagaaacat
      301 cgatacctat tggtcactat acaaattatg caattgaacc aacatgcggt ttagctacga
      361 tccatgattt tatcgggaaa gtggatgaac cacgctattt tatggatcct cgaaggatgg
      421 atgcccgcat tctctggttt accagcggat ttgtcgagta tcaggcacct aattttttaa
      481 atacagagga tacattggag atgttggaag tttctgtgga aatttcttct gaatttcctt
      541 tttcaaatga caattggcct tcagatatca ctttcagcct aaatggtgtg gagctaggaa
      601 catggacgag ccctggagat tttgctgata tccgcggtaa atatacgcct gattggtatc
      661 cagataatct taatcaatac ggcttgctga agacgatccg aattaccaaa cacttgacga
      721 atatgaatgg agaacccttg tcaaacatca cgatcaacga catccctaaa gagcaagaca
      781 cttggcattt gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc tgtactttat
      841 ttggtaaagg attcggtaat tacgatcagg atatcaaatt aaaagtttac tacagctgat
      901 atttttcttt tctattttat aaaacaaaaa cagccaatcc ctatgaaggg acggctgttt
      961 ttgctttatt gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg tcgtttatgt
     1021 tgtgtcttgt tccaccatgc ttcgattttt tcttgtgctt gagcgggaac ttcttttcct
     1081 tctagataat catcaatttc attatatgtg acacctagtg cgacttcatc tgcaatcatt
     1141 ggtttcccat cttcaagatc tgcagtcgga atttttgtgt ataatttttc cggtgcattc
     1201 aatgcttgaa gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg caaaatgtcg
     1261 gctccaccat caccaaattt cgtgaagaat ccagtaatgt tttctgcagc atgatctgtc
     1321 cctaatacgg ctcctgctct ttctccagct acggcatatt gcgtaatcat acgttgacgt
     1381 gctttgatat ttcctttgtt aaaatcgctg attcccacac ctgcttcgct taatacttgt
     1441 acttgtgcat caacagctgg tttgatattt actcgcagac taacatcggg ctggataaag
     1501 tcaagagcag cttttgcatc ttcttcatct gcttgttcac cataaggaag acgaacagcg
     1561 ataaattgat agttttcatc ttttgtttct tcacgcattt cttccatcgt tagttgcgcc
     1621 aaacggcctg ctaaagtaga atcttggccg ccgctgatac ctagtacaaa agttttaaga
     1681 aacggatatt ttttcaaata tgctttcata aagtcaatac ttttacgtat ttcttctttt
     1741 ggatcaattg ttggtttgac acctaattca ttgataattt ctgtctgcag gctcatttat
     1801 tctcctccta tgcttctgta tgaagttctg ctacgctttt tttcattcgt tccatgatag
     1861 ccattttatg gttccagcat tcagtagaca gatcgactgg gtatttttgc ggattcaagt
     1921 ctcgtttata ctcttcccat agtgaatcta gattttcttt tgtatattct ttgatttcgt
     1981 ttagtgcagg taattcgtaa actctttttc cttcgataaa gatatcttgt aaaactgggc
     2041 gagctgtaaa gtctcgaaca gttttattga taaacgtatg cactggatgg aacatgaaga
     2101 tagcttcttc ttctcgaggg tcttcatccc ataatgtcac gtagtctcct tcagatttcc
     2161 catcaaaatt tcgagtgatg cgccacactt gttttttccc tggagtagtt actttttctg
     2221 cattgcttga taacttgatc gtatccatca tttttccttc atcatcttcg atcgatacta
     2281 gcttgaatac tgcaccaagg gctggctgat cataggctgt gatcaatttt gtcccaacac
     2341 cccatacatc aattttagct ttttgcatct ttaaattcaa aatcgttgct tcatccaagt
     2401 cattcgaagc atagattttt gcttccgtaa atccggctgc atctaattgt tcccgaacac
     2461 gcttagatat ataggccata tctccactgt cgatgcgtac gccttggaag ttgattttgt
     2521 cacctaattc tttcgccacg cgaatcgcac ttggcacacc tgatttcaac gtatcataag
     2581 tgtcgactaa gaacacgcag tctttatggg ttttagcgta ggccataaat gcttcataat
     2641 catttccata agattggact aacgaatgag catgggtccc gctagctggt atcccaaata
     2701 tcttcccagc acgtacattg cttgttgcat ctgctccgcc gatatatgcc gctctagttc
     2761 cccagactgc tgcatccagt tcttgtgccc gtctagttcc aaattccaat agtggatctt
     2821 ctccgatgac agatttgata cgagctgctt ttgtcgcgat caacgtttgg aaattgacca
     2881 tattcaacag tgctgtttct actaattggc agtgcgctaa aggcccttcc acttggatta
     2941 atggttcatt tgcaaaaact agttctcctt cacgagcaga acgaactgtt gctttaaatt
     3001 caaaattttg taaatactcc aaaaaacctt caggatagac ttctaatcca cgcaagtaag
     3061 caatatcgga atcattgaac gtcagattct ctaaataatt gactaaccgt tccaatcctg
     3121 caaaaaccgc gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca aaaacagcat
     3181 gtaaatcagc tcgacctaat tcccagtatg tctgcatcat attgatttga tatagatccg
     3241 tatgcagcgt caaactgtcg tctgggtaag tattattcat cttgtattac tcctcttggc
     3301 aagttttcat ttcctaccca ttataacaaa aaaaattaat aggaaaagaa atcttccatt
     3361 ttttctgaaa aagcacatgt tttacggata tttcttgcta aaaatgaact ccatctgcta
     3421 aaactatcct ttcttattgg tatttaacct aaacgagaaa gaatacttat tttattcaat
     3481 gtatccgaga cacgaagaaa ttagcgtttt ctatttttct tacttgataa aataagtaga
     3541 ttttcctact acaatcgtag ccgttaagat accgatgatt gaacaaatat aaccaaatgt
     3601 ctctacaaca taatcaatgg caaaagtttt tgaagcaata atacgtcttt ccttttttgc
     3661 ttcttatccc caaagaaacg gatataactt attatgtatt gaattttttt ggaaaggcga
     3721 attatgacaa gatatacctt gcgttgaaaa aactcgcgga aaaaatatca cacgttttta
     3781 aaaagataca aagtaagaaa gcagctgtca atagaatgga aacaaaaatg gcgagacatc
     3841 aatcagtctc gccttgtttt ttattgtata cattagatat ggagcaggtt accataaaga
     3901 tgattattag tcgttattaa atagaatcct tcgtattttt ttgttctatc actacaatat
     3961 ctttatttgg acgtttgaat tgtattccgg cacgttctag ctcctgtttc actcctacat
     4021 ggactttgac atcttctcgt gtcattttta aaacgtgatg aatcgcatag tcgatttctt
     4081 gccttgtgat ttttttcttc gtattgatca tcacgacttg ctttttatca tatgtgtcta
     4141 ccaatagcag ttgtgttcga tctaacgaaa ataatttgta ataattaata gccgtattcc
     4201 caaaaaagaa ttccactact ttaggataaa atgtcttaat atttaaatta ctattcacag
     4261 gtgtttcgat tagcttcatc tcattcctct tttctatata gtcgatttag atccaatctt
     4321 aagttatgtt accatctttc ctagcattaa ggaatcggtt acaattgaac ctttaaagac
     4381 aaaaggattt accttttatc acttaatagt ataacataat ttcgtgagaa aaaaatacaa
     4441 ttttttcttg accatctcgt tacgtaaagg attatgctta catttgtcag gaggaaaaat
     4501 aatggagtac acaataaaga aaatgtcaga gatatcagga gtcagtccga gaaccttgcg
     4561 cttttatgat gagattggac tgctaaaacc agctaggatc aattcttcag gttatcggat
     4621 ttatggcaaa aaagaagtcg atcgtctgca acacatctta ttttatcgaa caatggcatt
     4681 caagttggat gatatccaag aggtcttaga caatccatct tttgaccacc agaaagcttt
     4741 aatcaagcat cagcaaatgt tgctggaaaa acgggcacaa atcgataccc tcttgacaac
     4801 tgtccaacag acacttgata tgtacgaagg aggaagaaaa atgagtgaca aagaaaaatt
     4861 tgaagggttt aaacaacaaa agttgaaaga aaatgaagaa tcctatggac aagagattcg
     4921 acaaaaatac ggaaaaaaaa aggttacagc atccaatgaa aaatggatga atatggataa
     4981 agaaacgtat gaagaaatgc aaactgttga aaagcagatg ttatttgatc tcactcttta
     5041 cttaaaagaa ccagaagatg aaagcttagc agaccgtatc ttccaagctc ataagaagtg
     5101 gttctcttac agctggcctg aatatcagcc aaaagcgcat aaaggactag gaatgatgta
     5161 cgaagccgat gaacggttca ctgcatatta tgacgagcga agtggtcatg gtgcagcaaa
     5221 agctttaaat gaaattatcc aaaaaaaggc gtaatctttt acatgatcca acaaaaaagt
     5281 acacgtaaaa tgacctaata acaaaacagc agacacccaa gttagatttt tatgctaact
     5341 tttggtgtct gctccattct ttcacaagtt ttttcatcct actttaaata tattcatcgt
     5401 tgaataaacg tataaggtat tttcacttta tccttttttt ctgtaagaaa caattgaaat
     5461 gccatttctc cgcaagcttc cagatggtgg tcgatggttg ggaaaccaag aagctcgctg
     5521 atcagcaaat tatcgcgtcc aatcaccact ggtgtcttat cttgcagata gctttgcaag
     5581 atgcctgctg caaccatatc cgaatttgta aagacaccat caactccaag ctctttaaaa
     5641 aagaagcctg cttgttttcc tttctctgca tctatacaat cccaaaaaac aagtgattca
     5701 tcaaaagatg gaaaaagttg atgacatagc tgcaaagtga tttttgagtt ataacttagc
     5761 ctgccacttc gccccaacgt gatgcccaat tgctttatac cttgctgctt caaataagta
     5821 agaccttctg taattgattg ttctcgatca ataaagacac agcttgcctg ttttcccttg
     5881 atttcttcac aaaaaactag aggtccatat ttttgatatg ccaataaatc atctagtgta
     5941 ttggcacgag acgtaatgat caatccatcg aaagctttag ctgcaaattc ttcaagatac
     6001 cttcgctctt tttctttatg atagcccgtc ggtaaaagtg tcactttgta atcataatca
     6061 aacgctgctt caatgatccc actgatcaac tgatcaaaat acggatgatt ggtataaggc
     6121 agaatcactc caatgctttt cgtttttcca taacttaact gtctggcatt tgcattaggg
     6181 atataatcga cttctttcat gaccgcaagt atttctttcc ttttttcctc atctacatat
     6241 ggatgtccat tgattacacg tgaaatagta gaaactgaat aacctgtgag ctttgcgata
     6301 tctcgaatgt ttgccactgc tgcttcgcca cctatacaaa aagattttct tgtaaatagt
     6361 ctaccacagc tggaccgcat tcaaaaaact gatacatttt tccttcatgc gaaaaactga
     6421 tccgctgccc gccccaatat atatcttttg tcaaattttc gatttcattc attggtattt
     6481 cttttactat atttctattc aacccttgaa aactgtacaa cgttaatacg gatcccttga
     6541 actcgacaaa cgcataaaaa taattgttcg ttgtgccatc tgttatctct acataagtat
     6601 tcattgcaag ccctcctttt gatactttga gtatagtgta tgaaacgcgt ctcatagcaa
     6661 ggagtttgtt ttgccaaaga aaagttttat tcctctttca ttattaccaa agtctcttat
     6721 tttaaattga atacattata atgatactgt aaatatatct ttattattag tgaatg
//
LOCUS       DAXWLF010000140         6745 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXWLF010000140 DAXWLF010000000
VERSION     DAXWLF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.142"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..805)
                     /locus_tag="ACGE4U_002204"
     CDS             complement(<1..805)
                     /locus_tag="ACGE4U_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFF8394322.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILD"
     gene            970..2700
                     /gene="spxB"
                     /locus_tag="ACGE4U_002205"
     CDS             970..2700
                     /gene="spxB"
                     /locus_tag="ACGE4U_002205"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFF8394323.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            complement(2886..3407)
                     /locus_tag="ACGE4U_002206"
     CDS             complement(2886..3407)
                     /locus_tag="ACGE4U_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFF8394324.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            3656..5278
                     /locus_tag="ACGE4U_002207"
     CDS             3656..5278
                     /locus_tag="ACGE4U_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8394325.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            complement(5407..6270)
                     /locus_tag="ACGE4U_002208"
     CDS             complement(5407..6270)
                     /locus_tag="ACGE4U_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8394326.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
BASE COUNT         2172 a         1346 c         1256 g         1971 t
ORIGIN      
        1 catccaaaat catcataatg ataaatccac tcatgacgcc aaaaactgca aaatgacgtt
       61 tgctggacaa tgtctgctgt gcttcaggga tcaattcttc caccacgaca taaatcattg
      121 ctcctgcggc aaaagcaagt gcataaggaa gcagcagctc cactttcgtt actagtagcg
      181 ctccaatcac tccagcaatt ggttcaacga ttccagaagc ttgtccgtaa acaaaggcct
      241 ttttacgact cagaccttct tgtcgcaacg ggattgatac agctgctcct tctgggaagt
      301 tttggatacc aatacctagc gccaccgaaa cagcggctaa tatagcggct gtcggattat
      361 ccgcagttgc tgctgcaccg aatgctaccc ccacggctaa accttcagga atgttatgca
      421 atgtaataga aaaaacaagc aagatcgtcc gttttaaatg ggtaggcaat ccttctgctt
      481 catgttgtgg tccaaaatgc atatggggca gtgttttatc tgccatatat aaaaacacac
      541 cgcctaaacc aaacccaata cttacaacta gccaagcaat atctccattt tcttctgctt
      601 ttgtaatagc cggatctaac aaagaccaaa aacttgcagc aatcatgaca ccagaagcaa
      661 atcctaacat caagtttagc acttcttttt tgatttcttt aaagaaaaaa accaacccgg
      721 caccaagagc tgtcataaaa taagtaaaag ctgtcccagc aagtgcttgc tgccaaggtt
      781 caagtctcaa tagccattca accatgccat tcccctctct tgaatctatc tatgtactac
      841 tctagcatat tcccaaaaac atgactacat ttttgtgaaa agacgaatta gttgggattt
      901 tcgataaaaa gttctatact ctagatacaa aaaatttatg tatcatcatt gctaaaggag
      961 tgaaacacca tgtcaaaaat caatgcagga atcgcaatgg taaaagttct tgaaagctgg
     1021 ggtatcgacc atatttacgg cattcccgga ggatctttca attcaattat ggatgcgcta
     1081 tatcaagaaa aaaccgaaat caactacatt caagttcgtc atgaagaagt cggcgcactc
     1141 gcagccgctg cagatgcaaa attaacgggg aaagtcggcg ctgtcttcgg ctctgcagga
     1201 cctggtgcga cacatttgat caatggtttg tatgatgcac agatggacca tgtgcctcta
     1261 gtagcgttct taggacaagt tgcttctaca tccatgaact ataactcttt ccaggaacta
     1321 aatgaaaatc ctattttcgc agatgttagc gtatacaatc gtacagtcat gacaccacaa
     1381 agtcttccgc atgtagtaga tgaagccatt aaagccgcct atgagcataa aggcgtggct
     1441 atcgtgacta tccctgttga tcttggtttt gaagaaattg acgaacaacc tttctccacc
     1501 gctcacactc acaaaacaag tgtgatatta ccagaagaaa aagatttgct agctgctttg
     1561 ccttatcttg aaaaagcaaa aaaaccagtt ctttatgttg gtcaaggaac gcgaaacgga
     1621 tttccgcaaa tcaaagaatt ctcagaacat ttttcagttc ctgttgtcgc ttccgttcta
     1681 gcaaaaggaa ttattcctga tgattatgaa aactttcttg gttttgctgg acgtgtcgct
     1741 actaaacccg gaaatgaagc attggcagaa gccgatctga tcttatttgt cggtagcgac
     1801 ttcccatttg gacgtgcatt ttttaatcca actgctgaat ttatccaaat cgatatcgat
     1861 gcgtcaaaat ttggacgacg ccatgaaaca tctttgtcga tcctcggtga tgcaaacaca
     1921 gctttgcaaa gacttgtcga gttaggggat gctcgccctg ctgatagctg gtacctagca
     1981 aatcaagaaa ataaagaaaa atgggtaaat tggttgaaat cttttgaaga tcgagaggaa
     2041 gaaccgattc gtccagaggc tgtctataaa gaaatcaatc gtattgctga agacgatgcg
     2101 attttcgtca cagatgtcgg taacacgact atccattcta ttcgtttatt gaatatgaac
     2161 ggtaaacaaa aacatacgac atctggatgg ttcgcaacta tgggtaatgg tgtgccagga
     2221 ggaattgccg cacaactaag ctacccagaa aaacaagtat ttaccttaag tggtgatggc
     2281 gggtttgcta tggtcatgca agatatcatt acacaagtca aatatcagct tccaattatt
     2341 aatgttgtct tttcgaatga ttcttttggt ttcatcgaag cagaacaaga agatacggaa
     2401 caaaagaaat ttggtgtatt tttagaagat gcagatttcg gtaaagttgg agaagcgctc
     2461 ggggcaaata gctttacagt cacagaatac tcgcaacttc gatttgcctt tgatgcagct
     2521 gcaaaatcag atcgtcctgt agtaatcgat gtgaagatca ataacaagcg cccattgcca
     2581 gtagaagatc tgcatttaga tccaagaaaa tattctgaag aagaaatcca agcattcaaa
     2641 gaaaaatatg aagtccatga tttgccagta ttgaatgaat tatatgaaaa gaggttgtga
     2701 caactgcgct atgcttcgag tattaagata aaatttgtgt atatagcttc tcacattttt
     2761 gcataaattt gtctcgacac ctaaaaatca cctcgtgcat atttgtccat gataaaaaag
     2821 ccggcttcca attacaacag taaaactgta atcggaagcc ggctttttta tttgtttgat
     2881 tttacttatc taaatgcata gacgatatgt gtgataccaa ataacataaa atagaatcca
     2941 actaaaaagc ttaatgtcaa cgcagaagaa attggattaa acaataagaa aattcctaat
     3001 aaaattccta aaatattgac gatgatcgta aaccaataat atccttcact tactcctctt
     3061 gccaagtcag cagtaaataa cgctaaaact gaatcagcaa taaaccatac tgcaaaaaca
     3121 aaaggcaatg ctgcgactcc tgcaccaata ttgaataaga aaaagacgcc aactagaata
     3181 tcgatgatcc cgattattaa gggcatcttt cctttatatc ctgttagctc ttttaatctg
     3241 ttccgtacaa acagttcaaa tatcccttta ataaaggcaa aaatcgcaaa gactactaca
     3301 attgcaacta aatttccgac agggtcttga aatgataaca atgagacaag tacaaataaa
     3361 attcccaaaa tcaatgagcc ccagtcgatg gttctttttt catccatttt caatccttct
     3421 ttcatttctt ttacagtttt atagtactcc ctcttataaa taacgtcaaa aattatgctg
     3481 ttgcaaacta ataaatccta tagtagctat tttcgctccg cttttatttt tatttttttc
     3541 attctgtttc acgtgaaaca caaagaatgt tcgattgcct tgaatctttt acctttcccc
     3601 tatataattc tataagactt gtcaaaacta ataatgataa aagtgaggaa aaaaattgat
     3661 tactgtaaat gatgtgagtt tgcacttttc agaccgcaaa ctatttgatg atgtgaatat
     3721 taaatttacc cccggaaact gctatggctt gattggtgca aatggtgcag gtaaatcaac
     3781 gttccttaaa gtgttatcag gggatctgca gccttccaca ggttccgtta ctatgggacc
     3841 agatgaacgg atggccgtat tgaaacagaa ccattacgac tatgaagact atactgtact
     3901 tgaaacagtc atcatgggac acaagcgtct gtatgaagtg ctgaaagaaa aagatgcgat
     3961 ttacatgaaa gaggatttca ctgacgaaga tggtattcga gcagctgaat tagaaggaga
     4021 atttgctgag cttaatggat gggaagctga accagaagca gctgttttac tgcaaggtct
     4081 gaacatccct gaagaattgc accatcagca aatgagtgaa ttaactggtg gacaaaaggt
     4141 aaaagtgtta ttagcacaaa cactatttgg taaacctgat gttcttttgc tagacgaacc
     4201 aacaaacggt ctggatatcc aatcgatcaa ttggctggaa gaatttttga tcaattttga
     4261 aaatactgtg atcgttgtat cccatgaccg tcactttttg aacaaagtat gtacacatat
     4321 ggcggatcta gattttggta aaatcaaact ttatgtagga aattatgatt tttggttgga
     4381 atctagtcaa ctagcggcta aacttcaagc aaacgcaaat gccaaaaaag aagaacaaat
     4441 caaagaactc caagaattta ttgcccgctt tagtgcgaat gcatcaaaat caaaacaagc
     4501 aacttcacgt aaaaaaatgc ttgaaaaaat tactttagac gatatccaac cttcttctag
     4561 acgttatcca ttcgttggat tcaaacctga acgtgagatt ggaaacgact tattgcaggt
     4621 agaaaatgta agtgtaacga ttgatggaaa gaaaatttta gatgatatat cttttaattt
     4681 gagcaaagaa gacaaggtcg ctttcgtcgc agaaaatgat ataacaacaa caacgctatt
     4741 caaagttatc atgggtgaaa ttacgccaga tactggaaca gtacgttggg gtgtgacaac
     4801 aagtaattct tatttaccaa aggatacaac gaaagaattc gatactgatc taacgatttt
     4861 agactggttg cgtcaatatg caagtaaaga agaagatgat aacaccttct taagaagctt
     4921 cttaggaaga atgttgtttt ctggtgaaga agtattgaaa ccagtgaatg ttttatctgg
     4981 tggagagaaa gtccgttgca tgctttcaaa aatgatgctt tcgaaagcta acgtacttgt
     5041 attagatgat ccaacgaacc atttagactt agaatcaatc acagcattga atgacggctt
     5101 gatggcattt actggctcaa tcttatttgc gtcacatgac catcaattta tccaaacatt
     5161 ggcaaaccgc atcattgccg tttcggataa aggtgtgatc gatcgagctg atacgacata
     5221 tgatgaattt ttggaaaatg cagatattca aaaacaattg aacgagttat gggcataatg
     5281 gcctatttag cttaaaaaat aaaaaaacag cgacctctaa gagataggat gctcatctat
     5341 cttttaagag tcgctgtttt tgttacaatc aatttctggg cttttttaca gaatgagtta
     5401 ctgcttttat cgactgattc ccatcgaaaa aatcgcgata cagccatagc ctgtgtgact
     5461 ggcgatcgtt ggtcctaagg gataaagctt cacatctttc atctttatct gctcttccaa
     5521 aagccgtttt gccttctccg cgctctctag atccccagcg taagccacat aaacgatttg
     5581 atcaagtggt gcaacgattg tttttaccgt ctcattgacg attgtctgga tcgacttgtt
     5641 tcttcctcga actttgtcaa cattcgtcag ccttccttct ttatccaccg taataatagg
     5701 ctttacactc aatagactgc caagagtagc acttgctttg gaaatcctac cgcctcgttc
     5761 tagatatttc agatcgtcta ctgttaccca tgaatggact ttcaaataat tactttccag
     5821 ccactctagg gtttcttcga tgcttttacc ttcttttttc aatcttacag cttccaatac
     5881 taaaagccct tccccaatac ttgcagcttt tgtatcaaaa acatagatct cagggtcttc
     5941 atattcttcc ttcagcaatt caaccgcttg aagcgcacta ctgtaagagc cgcttaatcc
     6001 actggaaaaa gccacatata aaacaggaat ctgtttttcc gcataagaac ggaaaaattc
     6061 taaatatcgg ccaacattga tttgtgatgt cgtaggcatc gcaccatttt tcagctgatt
     6121 catgtagcgg tcataattga atgtctttcc gaggtcatct actaactctt gatcattcaa
     6181 ttggagattc attgataaaa aatcaacttt ttcttttgct aatacatggt atggcagatc
     6241 acaacaagaa tctgttacaa tttgatacac atttttcaac cccttctcgt tacattttac
     6301 ccttatcgtc tattatagca aatctcttgc gctagacaca ctttaaccgg tttattttcg
     6361 caagttctaa tcaaaataag aggttgtgac agaagtggtc agcttcaaga aataagaagg
     6421 aatttacgaa aattgcttct caaatttttg tgaaatttcg gcttatttcc gtagaagcta
     6481 cttctggaac accgtgaatc agaggttgtg aaatcagcgt tttgacctga gtattagaga
     6541 aaaaaacgga aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca
     6601 gctgattgaa caccgtttac caagaggttg tgagcctgcc gtttagctcc gagtcacaag
     6661 gaacaatttc aaaaaacagc ttctcatgtt tgttgaatat tgtgacttgt gaccgaggag
     6721 ttggcaggag aacaccgttt agtgg
//
LOCUS       DAXWLF010000141         6723 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXWLF010000141 DAXWLF010000000
VERSION     DAXWLF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.143"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            79..852
                     /gene="sufC"
                     /locus_tag="ACGE4U_002209"
     CDS             79..852
                     /gene="sufC"
                     /locus_tag="ACGE4U_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFF8394327.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            865..2151
                     /gene="sufD"
                     /locus_tag="ACGE4U_002210"
     CDS             865..2151
                     /gene="sufD"
                     /locus_tag="ACGE4U_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFF8394328.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            2148..3383
                     /locus_tag="ACGE4U_002211"
     CDS             2148..3383
                     /locus_tag="ACGE4U_002211"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFF8394329.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            3370..3840
                     /gene="sufU"
                     /locus_tag="ACGE4U_002212"
     CDS             3370..3840
                     /gene="sufU"
                     /locus_tag="ACGE4U_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFF8394330.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            3845..5236
                     /gene="sufB"
                     /locus_tag="ACGE4U_002213"
     CDS             3845..5236
                     /gene="sufB"
                     /locus_tag="ACGE4U_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFF8394331.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5331..6473)
                     /locus_tag="ACGE4U_002214"
     CDS             complement(5331..6473)
                     /locus_tag="ACGE4U_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8394332.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(6512..>6723)
                     /locus_tag="ACGE4U_002215"
     CDS             complement(6512..>6723)
                     /locus_tag="ACGE4U_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394333.1"
                     /translation="DNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDNI
                     NKLEQTISSIGKKYTKKKMKGGDTI"
BASE COUNT         2119 a         1317 c         1430 g         1857 t
ORIGIN      
        1 agtaaggaag gtcattgcgc ttcattctca attagtctaa aatgagtgag aacgaaagag
       61 aatttggggg tataatgcat gtctgtctta gaaataaaaa atctacatgt gtcgatcgag
      121 gaaaaagaaa tccttaaagg cgtaaaccta acaatgaaaa ccggagaaat ccatgccatc
      181 atgggaccta acggcacagg aaaatctacc ttatctgccg ctatcatggg caatccaaac
      241 tatgaagtaa cagaaggcga aattttgttt gacgggaaaa atgttttaga cttggaagta
      301 gatgaacgtg cgcgacttgg cttattttta gctatgcaat atccaagtga gataccaggt
      361 atcacgaatg cggaattcat gcgtgcagcg atcaatgcga aacgtgatga aaacgacaaa
      421 atttctgtta tggcattttt gaaaaagtta gatgaaaaaa tggctttact aaatatgcca
      481 gaagaaatgg ctgaacgtta tctgaacgaa ggtttttctg gcggagaaaa gaaacggaat
      541 gagatcctgc aattgttgat gttagaacct acatttgcaa ttttagacga aatcgattca
      601 ggtctggata tcgatgcttt gaaagtcgtt gctaaaggag taaatgaaat gcgcggagac
      661 aatttcggtg cattgatcat cacccactat caacgattat tgaactatat cacacctgat
      721 gtcgttcaca tcatgatgga aggccgagta gtcatgacag ggaatgctga cctagctaaa
      781 cgtctggaag cagaaggcta cgctggtatc agtaaagaat taggaatcga ctataaagaa
      841 gaagaagcgt aagggggagc aagaatgaaa gaaaaaaact ggctcgatta tcttgacgcg
      901 gtaaatgatt tttctttaag taaaggcgaa cctgattgga tgagaacctt ccgtcaagac
      961 gcactagcga aagcagatga actgccattg ccgcatatcg atcgggtgaa attccatcgc
     1021 tggtcgttat ttgatgtgaa agaaacacaa accatcagtg aaacaggcac gattccggca
     1081 tttgatgcaa tgaaagacaa tcctgtgctt gtccaacaag gttcatggac gatttttgaa
     1141 caactgcctg tggaattagc agaaaaagga gtcattttta ccgacttgtt cactgcaatg
     1201 atcgaatatc cagaactagt ccaagaatat tatatgaaaa aagcagtaaa catgaacgaa
     1261 gaccaattga ctgcactcca tgtagctttt atgaacagcg gtattttcct gtatgtgcct
     1321 aaaaatgtcg tgatcgatga accactcgag tccttattca ttcaagatgg cgcatccgat
     1381 gagcacttct tcaagcatgt gttgatcgtg gcagatgaac atagtgaatt ttcttatctg
     1441 gaacgtttcc agacaaccaa agaacaagta gcaaaatcat caggtaatat catcgtggaa
     1501 gtcatcgcta aagctggttc aaaaatcaaa tattcagccg tagatcaatt aggagaaaat
     1561 attacttctt atatgaatcg ccgtgggcac attttacgag atgcttctgt cgactgggca
     1621 attggtgtca tgaatgacgg tcatgtgatc gctgactttg actcagattt agcaggagaa
     1681 ggtgcacacg cagaagtcaa aatcgtggca atcagctcgg gcagacaaat ccaaggaatc
     1741 gatacacgag taaccaataa agcccctcat acgatcgggc atattttgca acacggagtg
     1801 atccgagaaa aaggaacatt gacctttaat ggtatcggcc acatcttgaa aggggcaaaa
     1861 ggtgcagatg cccaacaaga aagtcgtgtc ttgatgcttt ctgataaagc acgtggtgat
     1921 gcgaatccta ttcttttgat cgatgaaaac gaagtaactg ctggacatgc tgccagtgta
     1981 ggacgagttg atccagaaga aatgtactat ctgatgagta gaggattgca taaagaagaa
     2041 gcagagcgct tagtgattcg aggattcctc ggttctgtct taacagcaat ccctgtagaa
     2101 caagttcgga aagaattagt cgaagtaatc gaagggaagt tgaatggatg atcgatgcta
     2161 aaaagctacg caatgatttt cctatcttat ttcaagacgt caatgatgaa cctttagttt
     2221 atctggataa tgcagcgacg acccaaaagc caaaacaagt actccaggca ttagcgtact
     2281 attacgaaca cgataacgcc aatgttcatc gcggtgtcca tacgttagca gaacgcgcga
     2341 cccatgatta tgaacaagca agagaaaaag tacgggcgtt catccatgca aaagaaacag
     2401 cagaagtgct gtttactcgt ggtacgacga caagcttgaa ttgggtagcc agaaccttcg
     2461 gcgaccgcta tatcaaagca ggagacgaga tagtgctctc ttacatggaa catcattcta
     2521 atgttatccc gtggcagcag ttggcaaaac gaacaggtgc tgtcttgaaa tatatcgaac
     2581 taacagaaga aggcttcctg gatatggagg atgcagcgaa aaagattacg gacaaaacca
     2641 aaatcgtctc cattgctcat gtatccaatg tcttaggtgt cttcaatcct gttcaagaat
     2701 tagctgaact tgtccatgaa aacggcgggg tcatcgtagt agatggtgct caatctgccc
     2761 cgcacatggc cattgatgtc caagcactag actgtgattt ctttgctttc agcggacaca
     2821 aaatgtgcgg accaacaggt attggtgttt tatatggtaa acgaaaatgg ttagaagaaa
     2881 tggaacctgt tgaatttggt ggcgaaatga tcgattttgt cgaattatac gacagtacat
     2941 ggaaagaact accttggaaa ttcgaagcag ggacaccaaa tatcgccggc gctatcgcat
     3001 taggccatgc aatcgattat ttgcaaaaac tcggtatgga caatatccat cgatatgaag
     3061 aagaactagc cgcttatgtg ttgccaaaat tacaagcaat tgatggattg actgtttatg
     3121 gtccacaaga cccgaatttt cggacaggag tcgttgcatt caatttggat gggttacatc
     3181 cccatgatgt cgcgacagct ttagacatgg aaggggtagc cgtcagagca ggtcatcatt
     3241 gtgcgcaacc gctattgaag taccttgaag tatcagcgac tgctcgcgcg agcttttatt
     3301 tctacaacac aacgcaagac gccgaccgtt tagttgaagc aatcatggca acaaaggagt
     3361 ttttccaaca tgggactatc taaattagac aatttatacc gtcaagtgat cttggatcat
     3421 tcttcccatc cgcatcatcg tggaagcttg actgactcga caagcaagat cgaattgaac
     3481 aaccccacat gtggagatgt gatcgaattg caagtcgatg taaaagacgg aatcatcaaa
     3541 aatatcgcct ttgacggcag tggctgctcc atcagtacgg caagtgcctc gatgatgaca
     3601 gacgccgtca tcggaaaatc aatagaagaa gcagaacatt taactgtaga ctttctacag
     3661 cttgtccaag gaaaagaagt caaagatccc gaccaattag gcgatgctgc tatgcttggt
     3721 ggtgtagcta aatttcccgc tcggatcaaa tgtgcgacac ttgcctggaa agcactagga
     3781 aaagcaatgg aaaacgatgg taccgcagct ttccatcatt accacaagga agaggagtga
     3841 gaacatgggc gtaccagaat tagaagaata taaattcggg tttcacgatg atgtcgaacc
     3901 ggttttcagt acaggagaag gcttgacaga agaagtgatc cgtgagatgt caagaatcaa
     3961 aggcgaacca gaatggatgc ttgaattccg tctgaaatct ctagaaacat ttaacaaaat
     4021 gccaatgcaa acatggggcc ccgacctttc agacatcgat ttcgatgcca tcaaatatta
     4081 ccaaaaacca agtgacaaac ccgctcgtga ctgggaagac gtaccagaaa aaatcaaaga
     4141 tacatttgaa aagattggta taccagaagc tgaacgagct tatctagccg gtgcttccgc
     4201 acaatatgaa tcagaagtcg tgtaccataa tatgaaagat gaattcgaaa agttaggaat
     4261 cgtctttact gacactgact ctgctttgaa agaataccct gaattattca aagagtactt
     4321 ttcaaaactt gtaccgccaa ccgacaacaa attagcagcg ctgaattcag ccgtatggtc
     4381 aggtgggacc tttatttacg tgccaaaagg cgtcaaagtc gatgttccat tacaaacgta
     4441 cttccggatc aatgcggaaa acacggggca attcgaacgt acgctgatca ttgttgacga
     4501 aggagcaagc atccactatg tcgaaggttg tacggcacct acttattcaa gtaacagcct
     4561 gcatgccgcc atcgtagaaa tctttacaag aaaagatgcg tattgccgtt acacaacgat
     4621 ccaaaactgg tcagacaatg tgtacaactt agtaaccaaa cgagcaaaag catacgaagg
     4681 cgcaaccgtt gaatggatcg acggaaacct aggtgcaaaa acaacgatga aatacccaag
     4741 tgtttactta gatggaaaag gtgcacgagg caccatgctc tccatcgcct ttgcaggagc
     4801 aaaccaaatc caagatacgg gtgcaaaaat gatccacaat gcaccaaata catctagctc
     4861 tatcgtctct aaatcaattt caaaagacgg cggagaagta aactatcgag gacaagtaac
     4921 ctttgccaaa aacagtgcgg gttcgatctc tcatatcgaa tgtgacacga tcatcatgga
     4981 cgaatggtcg aaatccgata cgatcccatt caatgagatc cacaacagcc aagtctcttt
     5041 agaacacgaa gccaaagtct caaaaatctc agaagaacaa ctctactatc tgatgagccg
     5101 aggcctgacc gaatctgaag caactgaaat gatcgtcatg ggctttgttg aaccattcac
     5161 caaagaactt ccaatggaat atgcagtcga actcaatcga ttgatcagtt atgagatgga
     5221 aggcagtgtt gggtaaacct gttataggag ctagaaccct tgatatataa gggttctagc
     5281 ttttttgttt tgcctgtata ctagaaatga ccagattttg accagctctt ttactgatga
     5341 gccatcatat cagcaaagtc ttgacctgtt ttcttaggtg cattatttgt aatatgtgca
     5401 tagatgtcta atgtcacttt actattttca tgccctaatc gttgttgaac taccttgaca
     5461 gattctcctg cttctaataa taaactagca tgtgtatgtc taaacccatg aggtgtaata
     5521 cgaggcaagt tatatttttt caaaatatat cttaaccaat cattaacagt gtttggaatg
     5581 tatagagtgt ttttaactgt tgtaaaaaca tgttgctctt tagagctagt attgtatcca
     5641 agttttaaat actcttcttt ttgctgtaat ttccagttgt tgaggataga aagagtctca
     5701 gtatctaaag agataactcg tctagaagct cttgttttcg gagattgaat tataacttta
     5761 ccaaattcat ctacagcaag agttttattg acatgtagtt ctttattaaa cgtgtctatg
     5821 tctttccact gtaaagcgag tacttcactc tttctcatac ctgtgtatgc caataatctg
     5881 aagaaggcgt attgtttcat gttaccaaaa tctttaaaag catccagtaa attattaagc
     5941 tcctcttttg agtaaaattt acgatttttc tcatactctt tttttcgtgg aagaagtgtt
     6001 ttttccatag gattattgtc aatatattgc attgcaaccc cgtagcgtaa aatttgtgca
     6061 gtctgttttc tcatataatc gtaactttca tatttactgt gccattcatc taccactttt
     6121 tgacaatatg ggacagagat attcgatagt ttaagcttac caaatgctgg aagtatttga
     6181 ttatttgcgt atcttactga gattgcgatt gttgaagctt taacactggt acgctgatga
     6241 gttatccatt cattatacag gtcttcaaat gtcatatcag gtgctttctt tttaacaccg
     6301 tataatattt gatgtctgta ctcgtcataa tctttttgag cttcttgttt gctactaaaa
     6361 cctcttctcg tagtatttat tttttcacca gttcgtggat cggtggcaat gtgtccttta
     6421 tacatccacg ccacttgacc gtttttcttt ttgtactttg atatttttgc cattaaatca
     6481 tctcctctta gtttacaagg agtacttacc actagatggt atcacctccc ttcatttttt
     6541 tcttagtgta ttttttccca attgatgaaa tcgtttgttc tagcttgttt atattatcag
     6601 taatttctct cacagcttct tcgggataag acatgtcttt caattgaata ggtagccatt
     6661 catctaccat tctttctagt agttcttcta tactgctagt gaggaaaaga atgttattgt
     6721 caa
//
LOCUS       DAXWLF010000142         6610 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXWLF010000142 DAXWLF010000000
VERSION     DAXWLF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.144"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(55..216)
                     /locus_tag="ACGE4U_002216"
     CDS             complement(55..216)
                     /locus_tag="ACGE4U_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFF8394334.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(213..515)
                     /locus_tag="ACGE4U_002217"
     CDS             complement(213..515)
                     /locus_tag="ACGE4U_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394335.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(508..672)
                     /locus_tag="ACGE4U_002218"
     CDS             complement(508..672)
                     /locus_tag="ACGE4U_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394336.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(677..946)
                     /locus_tag="ACGE4U_002219"
     CDS             complement(677..946)
                     /locus_tag="ACGE4U_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394337.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(958..1707)
                     /locus_tag="ACGE4U_002220"
     CDS             complement(958..1707)
                     /locus_tag="ACGE4U_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394338.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(1710..2396)
                     /locus_tag="ACGE4U_002221"
     CDS             complement(1710..2396)
                     /locus_tag="ACGE4U_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFF8394339.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(2402..3073)
                     /locus_tag="ACGE4U_002222"
     CDS             complement(2402..3073)
                     /locus_tag="ACGE4U_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFF8394340.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(3066..3407)
                     /locus_tag="ACGE4U_002223"
     CDS             complement(3066..3407)
                     /locus_tag="ACGE4U_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394341.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(3585..4283)
                     /locus_tag="ACGE4U_002224"
     CDS             complement(3585..4283)
                     /locus_tag="ACGE4U_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFF8394342.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4370..4840
                     /locus_tag="ACGE4U_002225"
     CDS             4370..4840
                     /locus_tag="ACGE4U_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394343.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(4883..5062)
                     /locus_tag="ACGE4U_002226"
     CDS             complement(4883..5062)
                     /locus_tag="ACGE4U_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394344.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(5049..5387)
                     /locus_tag="ACGE4U_002227"
     CDS             complement(5049..5387)
                     /locus_tag="ACGE4U_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFF8394345.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(5403..5606)
                     /locus_tag="ACGE4U_002228"
     CDS             complement(5403..5606)
                     /locus_tag="ACGE4U_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394346.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(5621..6367)
                     /locus_tag="ACGE4U_002229"
     CDS             complement(5621..6367)
                     /locus_tag="ACGE4U_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFF8394347.1"
                     /translation="MNTPQIFSFEQHEVRTFLENDIPYFVANDVAKTLGYKNPSKATN
                     DHCKKSIETWGNDSLGRRQKFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
BASE COUNT         1740 a         1367 c         1043 g         2460 t
ORIGIN      
        1 attctgactt ggttaattca gcaattcttt tcattgctgc tttcttatcc acgctcattc
       61 ctccaatcga tggatttccc ttcttaaatt ctctatgtgc aaatcgattg cctttctcgc
      121 cgtttcattg accatcactg cctttgttcg ttccagatcg tcaatctcac gctgaaggct
      181 tcgaatacgc atttgaatca cttcttctgt tgtcatgatg attcctccac gtatctaaac
      241 gttctcttct tagcgtctgt gtagccacac cttgctcttt tcctcacgat tttctcgtgc
      301 aagcctgtga gacttgctaa ctgttcagct gtccctatca ctagaagttt gtcaccgtgc
      361 caaattgcga tttttcgtgg tcttggcttg ttgctcttgt ctgcccacat cgctcttcca
      421 agcctcatca cttctgaagc agcttctttg tcattttgcc aatcttctga ataagtcaat
      481 tcgataattc gctgcattgc cgctttctta tccatcccga cattctcctt tcagtaattt
      541 gagtacttga tcaagtgcgc tctcacgtcc gccatggaac gtgttgagcc acttgtcttc
      601 gtacgataca ctttgtctta aagcttcttg atgcattagt tcaatctgtg ctgtaaatgt
      661 ctttaggtcc atctgattac acctgctcaa gttcattaag atgtttttgc aagcctttaa
      721 cacaatcaac aaatagtaat ttggtatatg ctaaatttct taattgtgtt gtatcgatat
      781 aaagtgcgaa atagtatctg agcttactcc aactcgaacg atcattctta attccttcaa
      841 ttccagcttc ttcgagttga tcatatacgt ctctcaggac gtctaattcc tcgccctttt
      901 tataattcgc tatttcatta attagttcta gataatcgat cttcattttt ccacctctta
      961 gaatggtgct tttgattgtc tattagctcg ttctagcgct tttttcttga gataggcttc
     1021 ttggtcgatt gcccattcag gaagcttctc tcgtcttcct gtgcgcttgt atccactgct
     1081 tgcgttctta ggttcacttt tttctttcct tgcccaactt cgaatagttg ccaaatagtt
     1141 tttataagtc ttaccagatg attcacaata ctctgacagt cgttcgattc gctcttggta
     1201 atcattaggg aattctgttt tgagtttctc catctgctca tctgacaaaa gaacattttt
     1261 atactctccg tatttatgac ggacgggctt agccttcgat tttttcgaag gcggtaactc
     1321 tcttatatat tcttttgtat tattaaatgt attattaata gatgtattat tatctttgac
     1381 tttttcgtca ataggggtat tgcgtttttc gtcaataggg tattgattaa ttcgtaggta
     1441 cctattgatt atttgattgg taccctcttt gtaaatgatt tcccgattca agtatccaaa
     1501 cttaatcaaa tcacttaccc atcgcgatat ggtctcttta ttcacaccat ataaatctgc
     1561 aaagtactca ttgcctgccc aacaaaagcc tctttcatta cacaaggccg ttatctctcc
     1621 gtataacaac ttagtatttg gtttaagtct tttgtcgtac cttacgttgg ctggtataat
     1681 cgcataataa cttcgatgtt ctgtcatttt taccctccaa tatttaactt tttgattgtt
     1741 ttctggttta atttgatccc tttgatttga tatttatttt tgaaattaat cacacctatt
     1801 ttgtgcttct ctgtgtgatg gattctgcag agtgctgcaa atgtgtactc tgcatgatca
     1861 acttctttgc gctttcgtct tcctagcgct ttgtcaaagt gatcgatgtc agctcctgtt
     1921 ttgccacaga tgcagcagac tctttttgtg atgcatttgt agaagtaata ttcttgattc
     1981 gcaggtaaaa tctcatagcc ttctttgaaa ggaatatgat gttcaaagat gaaatctaag
     2041 atgatatttg ctaagacatt ggcatcactc acagttgtat tcgattcgtc tttcaggctt
     2101 attttgcgcc ctgtgacacc ttcaaaacga aagtagaaga attccttcca gaagtccgtt
     2161 ggcatgcctg tatcgataaa aatatcgcct atcagcgcat agatgaagtt tcgttgctgt
     2221 acggtgaaac gtctaggatc aataaaacga atttcaatga ctcgatcacc atcgtagccg
     2281 tcatacatcg tctttagtcg atcaatgttc acttcctcat tgatggttgc gcttatgtct
     2341 tttcctttga actttttcag aaccgctgaa tatgaatcga ttaatggttt aaacactcat
     2401 atcacttctt atctaattct tttctcttag ctgctattgc tcgctccatc aaggcacatt
     2461 gctcatagct taactgttca atagtttcaa cgttatcagc taagagccct aatttatctg
     2521 tctgctcatt aacatattcg attaaggttt tggtcatatc tttacccatc tgctcattga
     2581 aagcttctag aatcgtctct agcatgttta atttctttgt atcgattcta ggtggtgttg
     2641 gaatatcttc cccttgaaat acatataatc ccagtccgtg tagagccaat gctttcacaa
     2701 agcatcgctt caatgagtta ttgatttgca ttgcatttgg tttaacaact ggttggtttc
     2761 gataatctaa aacaggaaat aattcggttt ccgtgtgtcc tttaaccgtt actgagacag
     2821 atacataagt cccagtttca tccataagaa aaggtttata ttcctcaaca agaaagtctt
     2881 gatgagttcc agaaacaacc ctgtagtgtt tgtactcatt aatagttacc gttgcctgtg
     2941 gatcattctt tttcataatc tcccacgcgt gagcccaaga taaataatca aaatttcctt
     3001 ttttcttgag aattttattt aacttacgac taaaaagttt ttcaaagttc gttgtccctt
     3061 tgatttcact catcaaattc tgcctccatt tcagcaatgt atttcttacc tggtccgtaa
     3121 taagagatat caatcaagtt atctctgtcg tactcttcta gcgcatcaat caagccatct
     3181 tcgatgacgt aaatatattc aggtttattc gaatgcttcg atagatggat aagataaaca
     3241 tgatcccaaa tagttacatg gttgcctaaa tcatcttgat cccaagctag ttcttcattc
     3301 gtcaaaagat ttcgtctgat ttttcgacca cttgtttcct caattttcgg cttgccccaa
     3361 tcaggatcaa tcaaatattg atctagagtg gaaagttctt tttccatatg ctaaaatctc
     3421 cttatgatgt gttttctttg tgactctttg cttgccggcg gagtcacttt tttatttgtt
     3481 gccatgcttt ttgcttgtca atatgttgtt ggcttaggat gattggttta tagtatttcc
     3541 accagcaatt agcaattgcc gtccctattc ttagcgcttc agctctattc attgtcatca
     3601 ccaaaaagtc tctgttgtct gttcagttga tcaatttcca tacggatcgc agtttctggc
     3661 aaccacattt caataaatga aacagcatca tcgaatctct tacgaggtaa ctcgccatat
     3721 cttgggattg aaaaggtacg tttaaattca gaccaaaatt ttgagaatac ttttttgctg
     3781 atttcttcat aagctcggct ttcttttccc cctagaactc ccataacttt catatttcct
     3841 ttttgcttaa tttcaaactc ttgttgtccg ctaattcgca tagtatcttt aagcatggaa
     3901 acatcttttt taacatcttt catttcttct aattgataga tcatcatatc ttcaattgtt
     3961 tgaggaacag tattcttgcg aataacatct tccatttcgt tgaatgcttc aatgtatttt
     4021 tgtttgaagt agatagcttt ctttcctgta aaccccatag ccagcaagaa aaatccatct
     4081 ctactaatga agaaaactcg tcgatttctg ccatatgaat ctggttcatt accttctaca
     4141 aacatctgtc caaaattgga cacatctttt tttagtgcat caatatctct taaaacatgt
     4201 tgatgttttt tctcgaagct ttctgccact tgtaagctcg tagtcacagc ttctttattt
     4261 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat ttagttaaaa
     4321 actggtggtg tttattttga atttttttca cttaacaatt tttcaatgga tgtcaataat
     4381 aagttttata ttgagtacgt ttttaacagg gataaaaata aaaggaatgc gccctcaact
     4441 agaagttgaa cttaatgctt cttacttcgc tgcagatatg atttatacaa aagtaatcat
     4501 ttctaactat tcaacagaac cagctatgtt agttaattta gaactttcat ccgctaactt
     4561 aaaccgcaga tggtcagcta ctccatttaa gaaattaatt gctaaaggag gatcagtcga
     4621 tgataataga atatactctg aagctgtacc gctaaatatt cctcctaaaa gtgctatatc
     4681 ttgttacctt gcatttgaag tagggaaaat taattttaat aaactcctta atcaccaaac
     4741 aaaaatgata tttactctaa atagaaccca aatccacaaa attgttgata tcaaaaatac
     4801 aaacttccca atagagaaat tagtgaaaga attaaactga gtaatagtaa tatacattct
     4861 agtttctctt ttctattcat ttttagtcag ccccctcggt tggctttttc gctctgtact
     4921 cagcttcagc ttcatcaagc cccataaaaa tccaaaccat gtaaacaatc gttcctatca
     4981 acgcttgcct gcttccccaa agtcctaaag cgtagatgat tagcggtgcg ctgaacacta
     5041 gcgctctatt aaattttccc atccgcttgc ctccttaaga tttccgaaaa atttgtttct
     5101 aaaaattcaa gtgttttact tcttaaaaat agatatgtgt ctcttccttc aactggataa
     5161 tagacaaatc catttttgtt tttctcgata tcgataatat ttctatatct tgggttcttt
     5221 aaaactctag aagtaaacca atcatatttt ctgttaatcc gttctagcac ttctggcaac
     5281 gtcatccatc taccagtatc atcagctttt tttaactcct cataatccac ttgggagata
     5341 attacatagc cttctggaat tgggattttc gcttctagat attgcattag ctgttcctcc
     5401 ctttacaatt cgtacatagt aataatcgaa tctatgattc tatttgcttc tgcagaagtc
     5461 tttttaccgt ttaaaattaa agataagtag cttttgctaa ttccaaatct ttcagcaagc
     5521 atggtgtaag ttaagaactt cgaactttcg acatattctt tgattttttc tctatctcgt
     5581 tgagtgattt ctgcaatgtc agtcatacta aaactccttt ctaaccaatt tcctctaaat
     5641 ccatttgagg gtaatagccc tctgcgatta ataagttgta aataaacact cgccctttct
     5701 gtgtccattt ggtattcatt acaactttag tgccgccatc ggctttcgga atctcacttg
     5761 tgtgcgattt tgtgtatcct tggttcatat gttttcggta aagtatccat tgtccgctaa
     5821 ctttatgttg aacacctaac tcgtttagca atttgtttag agcaattgcc gacattccgt
     5881 aatcagctgc aatttgagaa gtagctaccg tatctgtcga tgaaagaatc gtatctaagt
     5941 agctaatctt tggttcgtac tcggcaattt gttgttcgag taattggttc ttttcttcta
     6001 aatctgctgc taaacgcaat gcttgtgcaa aactttgagg tacgtttgaa tagctacctg
     6061 tttttctgat tgttggaagg acttcttcca ttacccaggc ctcaaatttt tcagcgcttg
     6121 gtaagttcga tttaataatc aagcggtaaa catctgattc tggaataact ttgaattttt
     6181 gacgacgacc taacgaatcg ttaccccatg tttcaattga ttttttacaa tgatcattgg
     6241 tggctttact gggatttttg tatcccaaag ttttggcaac atcgtttgct acgaaatacg
     6301 gaatgtcatt ttctagaaac gttcttactt catgttgttc aaaactaaaa atttgtggtg
     6361 tgttcatttt tgttcattcc tttctttgat ataattttct tatcagtaag tggtctactg
     6421 aaataatgca taaggtggtg aataagtatg aataaaaaaa ctgatgaaat cgctgcggat
     6481 ttagttgttg catggctaaa tcacgaatca caagtatcta ctaaaggcga atcaatttcg
     6541 ccaaaagaaa ttgctcaagc ttatttagat attcattatg ctgttatcta tggtcagctt
     6601 ccagaagacc
//
LOCUS       DAXWLF010000143         6420 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXWLF010000143 DAXWLF010000000
VERSION     DAXWLF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.145"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            199..390
                     /locus_tag="ACGE4U_002230"
     CDS             199..390
                     /locus_tag="ACGE4U_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394348.1"
                     /translation="MDTQRIVLETEEKEFIETVISFVTEKGWTISNLQNAVSKVEDYM
                     KKNATLTELTIGMPAQIKD"
     gene            464..691
                     /locus_tag="ACGE4U_002231"
     CDS             464..691
                     /locus_tag="ACGE4U_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8394349.1"
                     /translation="MTQELINKNDLDSFLIGYVPKRYLTQKEAVHYTGTSAGTINEWV
                     KKGLKVIIFGENSRPKYDIKDIDEFMSKYKV"
     gene            704..877
                     /locus_tag="ACGE4U_002232"
     CDS             704..877
                     /locus_tag="ACGE4U_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394350.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(991..1398)
                     /locus_tag="ACGE4U_002233"
     CDS             complement(991..1398)
                     /locus_tag="ACGE4U_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394351.1"
                     /translation="MSFDNTITISIILALVALISPWITAVINNKHAESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKILPILNKSLEKL"
     gene            1560..1784
                     /locus_tag="ACGE4U_002234"
     CDS             1560..1784
                     /locus_tag="ACGE4U_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394352.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            1794..1988
                     /locus_tag="ACGE4U_002235"
     CDS             1794..1988
                     /locus_tag="ACGE4U_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394353.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDVILEYVQQHYPKIELGGI"
     gene            1991..3013
                     /locus_tag="ACGE4U_002236"
     CDS             1991..3013
                     /locus_tag="ACGE4U_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFF8394354.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGENLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKGSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            2976..3791
                     /locus_tag="ACGE4U_002237"
     CDS             2976..3791
                     /locus_tag="ACGE4U_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFF8394355.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKTFTPIIAAVSKQTPSEARLITI
                     DQDNINYELVMLKEKIERIVRVKNGEEKPNHCGLCEYCRGNLPITGFTSMDDL"
     gene            3807..4592
                     /locus_tag="ACGE4U_002238"
     CDS             3807..4592
                     /locus_tag="ACGE4U_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394356.1"
                     /translation="MTGGWIKLYRQLLEKPIWQESTPEQKVILITLLSMANHEEREWE
                     WQGEPYKASPGQFVTSLDSIRKKCGKGISLQNVRTALKRFEKYEFLTNQPTNKNRLIT
                     IVNWEVYQSKDNDLTKELTSNQQATNKQLTTNKNDKNDKNDKNDNNPRNSRKTREYAD
                     DDPNKKLAILLLKLIRKNQNIKEPDLDKWANTIRLTIESDKRTGREVQDMIVWATSND
                     FWSGVILSPTSLRKHFDKMAIQKNKRKQQNISNDELPETGEDW"
     gene            4592..5440
                     /locus_tag="ACGE4U_002239"
     CDS             4592..5440
                     /locus_tag="ACGE4U_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8394357.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            5437..5679
                     /locus_tag="ACGE4U_002240"
     CDS             5437..5679
                     /locus_tag="ACGE4U_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394358.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            5822..6190
                     /locus_tag="ACGE4U_002241"
     CDS             5822..6190
                     /locus_tag="ACGE4U_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFF8394359.1"
                     /translation="MATRSKYGNKKHEVDGITFDSIAEAEYYDLALWQAEANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHK
                     YQVPLILAKKYRNTFKEERF"
     gene            6205..6366
                     /locus_tag="ACGE4U_002242"
     CDS             6205..6366
                     /locus_tag="ACGE4U_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFF8394360.1"
                     /translation="MTTEEVIQMHIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
BASE COUNT         2383 a         1043 c         1362 g         1632 t
ORIGIN      
        1 tgaatatcta aataagcttg agcaatttct tttggcgaaa ttgattcgcc tttagtagat
       61 atttgtgatt cgtgatttag ccatgcaaca actaaatccg cagcgatttt tttattcata
      121 cttattcacc accttatgca ttatttcagt aggccactta ctgataagaa aattatatca
      181 aagaaaggaa tgaatagaat ggacacgcaa aggattgttt tagaaacaga ggaaaaagaa
      241 tttattgaaa cggttattag ttttgtaacc gagaaaggct ggacgatttc taatttgcaa
      301 aatgctgtat caaaagtgga agattatatg aaaaaaaatg ccacattaac agagctgaca
      361 attggcatgc cagctcaaat taaagattaa tagattctgc agaagcaaac aaaattatag
      421 attcgattat tactatgtac gaattgtaga gggggaaaac gaaatgacac aagaactaat
      481 caacaaaaat gatttagatt catttcttat aggctacgtt cctaaacgct atctgactca
      541 aaaagaagcg gttcattata caggaacgtc agcaggaact attaacgaat gggtaaaaaa
      601 agggttgaaa gtaatcatct tcggtgaaaa cagccgtccg aaatacgaca tcaaagatat
      661 cgatgaattc atgtcgaaat ataaagtcta aggaggtaag cggatgggaa aatttaatag
      721 agcattagtg ttcagcgcac ccctaatcgt ctatgcttta ggactttggg gaagcagaca
      781 agcattaatc ggcacgatcg tttacatggt ttggattttt atggggcttg atgaagctga
      841 gtacagagca aaaaagccaa ccgagggggc tgactaaagt gtggttctgt ttattaggtg
      901 tttatttcgt ggccgtttta gtaaataact acatgagaaa acgtggcgaa tactggtata
      961 catcctatgc ggttctagta tgtctaatgc ttacaatttt tctaatgatt tattcaaaat
     1021 aggtaatatt ttgtcaatca aataagtttc aagagatttt tgttctgcat cagtgttgcc
     1081 gtaaccaaaa cggcttaaga aaattttcat aacctctata tcttcatttt gaatatatgg
     1141 caggactgca taacctgatg cttttacagc agatatattt ttatcagtgt tactaccaat
     1201 acaaataccc acattgttga gaaatgttga gaaagttgtt tgtattgttt gggtttttga
     1261 atcgtgtttt tgtaattcaa tttctttatc cttcatcgat tcagcatgct tattatttat
     1321 aaccgctgta atccaagggg atataagagc tactaaagct aggataatcg aaattgtgat
     1381 cgtgttatcg aaactcattt tttcaccacc agttttttac ctaaattata tcaaaaagga
     1441 gagaagaaaa aatgcaagaa ttagtaattt tgaaaaataa aaaaagacac gaacgccggc
     1501 aagcatgatc gtgtccaaaa gaaaaatctt ttaaaccatt ataacacagg aggaaataaa
     1561 tgatctcagt taaagggtta ggcgatgaaa tattcgaagc aatgatgagc aaagcacaac
     1621 aggacatcaa agaaaaaatc atttctgctg cagcgcaagg acaaactagc tgcactgttc
     1681 gatcgagagg aacgacacca agttttcttt tggcgctaga agatgaaggc attttgaata
     1741 tcaaacgaag agatggcagc gtcagattgt tttggcaatt ttaggaggcc gtgatgaaag
     1801 actttgattc tttaggtgcc agacagcaac caccaaatga agctagtcct gtcggagttg
     1861 attggcaaga aaatccgtta tatccaggtg atacttgcta tctaacagaa gaaggttatg
     1921 taccagtaga cgtaattcta gagtacgtac agcaacatta tccaaagatt gaattaggag
     1981 gaatttagga atggcaaatg atttaacaca aacaacacaa cgttcattag atgagcaggt
     2041 aatcagtaat cttggacggt tacaagaaca aggtttagaa atgccaccag gatatagtcc
     2101 acaaaatgcg ttaaaaagtg cttttttcga actcactaac aatacaggag aaaacttgtt
     2161 gcagatggct gcaaacaatc aagaaatgaa aacctccatt tctaatgcac ttttagacat
     2221 ggttattcaa gggttatctc cagcaaaaaa acaatgctat ttcattaaat acggaaacaa
     2281 ggttcagcta atgcgttcat attttggaac aatggcggtc cttgatcgtg taactggagg
     2341 ggcagatatt acgcctgtag tcgttcgaca aggtgatgag tttgaagttg caatggatgg
     2401 accaaacatg gttgtcaaaa agcatgaaac aaaattcgag aatttagaca atgaaatcat
     2461 tgcagcctat gtagtcatca agttaacaaa tggtaaagag accacaacag tcatgacgaa
     2521 aaagcaaatc gatcaaagtt gggctaagtc aaaaatgaaa ggttctggac cgcaaaagga
     2581 atttccagaa gaaatggcta aacggacagt aatcaatcgg gcagctaaaa cattgatcaa
     2641 cacaagcaac gacaacgatt tattagttca agctgcaaaa gatacgttag aaaatgaatt
     2701 tgacaatgat cggaaagatg taacgccaca aacagaaaaa gtggctactc tcgaacaaaa
     2761 attcttttca aacaaaaaga ttactgaacc aatacaaaaa gaaccagatc cgattgtgat
     2821 tccagatgat atccaaaatg aagtaactcg agttgctgat gtacctgccc atccagaaat
     2881 agagcaagca catttgatcg aaaatgagga tatcaatcca attcaagaag aattgataga
     2941 tattccagac tttggacgcg aggaaggtgt agaagatgtc tcagaatttg aagacgacga
     3001 gtaccctttc tgatgagaat tattactcca atgaagctga ctggcagtac atgtcaacat
     3061 cacaatataa gtccttttta aaatgtgaag ccgcagcctt agcaaagttg aaaggcgatt
     3121 gggcaccgac gtcggatccc aaagccttac tcgtaggaaa ctacgtacat tcttattttg
     3181 aatcaaaaga agtacatgaa gcgttcaaag aagaaaataa atccaaaatg ttttctagta
     3241 ggaagccgta tggattgttg aaagatttcc aaattgctga acaaatgatt gaacgactta
     3301 agcaagaaga agcattcatg aacatttatc aaggtgagaa agaaacgatc gtgactgggg
     3361 aactatttgg aacgacatgg aaaggcaaga ttgattgctt gaatgttgag gatgagtatt
     3421 ttgttgatat caaaacaacc aaagacatgc atgagcggaa atggaacgaa aactacggat
     3481 caagagaaac gttcattgtc aattttggtt acgtacttca aatggcaatc tatcaagagc
     3541 ttcttttaca gcaatacgga aagacgttta caccgatcat tgcagcagtt tctaaacaaa
     3601 caccaagtga agcaagactg atcacgattg atcaggacaa tataaattat gagctggtta
     3661 tgttaaaaga aaaaatcgaa agaattgtca gagtgaagaa cggtgaagag aagccgaacc
     3721 attgcggttt gtgcgagtac tgtagaggta atcttccaat tactggattc actagcatgg
     3781 acgatttgta gagtggtggt gatgaaatga ctggaggatg gataaagctt tatcgccaat
     3841 tactcgagaa acctatttgg caagaatcta caccagagca aaaagttatt ttaattaccc
     3901 ttctttcaat ggcgaatcat gaagaaagag agtgggaatg gcaaggcgaa ccgtacaaag
     3961 caagtccagg tcaattcgtt acttctctcg atagtattag aaaaaaatgt ggtaaaggta
     4021 tctcattgca aaatgttaga actgctctaa agaggtttga aaaatatgaa tttttaacaa
     4081 accaaccaac aaacaaaaat aggcttataa ccattgtcaa ctgggaggtt taccaatcaa
     4141 aagacaacga tctaacaaag gaactcacaa gcaaccaaca agcaactaac aagcaactaa
     4201 caactaacaa gaatgataag aatgataaga atgataagaa tgataataat cctcgcaact
     4261 ctcgaaaaac acgagagtat gcagacgacg atccaaataa aaaattggcc attcttttat
     4321 taaaactcat tcgaaaaaat caaaacatca aggaacctga tttggataaa tgggcgaata
     4381 cgattcgttt aacaattgaa tctgacaaac gaactggtag agaagttcaa gacatgatcg
     4441 tgtgggccac aagtaatgat ttctggtcag gtgtgatttt gtcaccgact agcttaagaa
     4501 agcatttcga taagatggct atccaaaaaa ataaaagaaa gcaacaaaat atttctaatg
     4561 atgagttacc agaaacaggt gaggattggt aatgaataaa aaactaagtg caatggctgc
     4621 accttacggc ggattaaaaa cagcagatca taattgccca aaatgtggtg atccattgta
     4681 tatctggaaa acaaaaaata aagatggtac tgatcgatgc ggtcctacat gtatcaataa
     4741 gatttgtggt taccgagaaa tggtaactaa aaatcaaaaa gaagctatcc aaaaagcgaa
     4801 tgaagcaatg aaaaaggacg ctatcaatcg aatgcttaac agttcaatga ttacagatga
     4861 tgccatatgg accttcgatt ttgatggata caaagtagtt gatcaggaaa cagcacaaat
     4921 aaaagcgatg gctcaagaat gggccaaaaa aattgtaaat ggtagcacga tccatgcagt
     4981 gataaccggt agaacaggag ctggaaaaac tcatctaggc gctgcagtga ttaaagaagt
     5041 catgagggca tctaattata aaattgcttg ttcatttatc agttatcgag aacttttaga
     5101 acagttgaaa tttgcaatga atgatccaga agcaagaaaa gccgtaacag gttcgctgat
     5161 ggctgagata aagaaatcag atttcgtggt aatcgacgat ttgggtgcag aactaggaag
     5221 aatggaagaa aacaatcagg caacgcctta tgatgttgat gttctcacat cgctcacaga
     5281 agctcgtcta aacaaagcta cgatattcac aaccaattta tcatcgaagc aattaaaaca
     5341 cgcgtacggt gagcgagtgt tctctcgtgt aatgaatgga acgaaaggga acatagctgt
     5401 attcaaaaca acgacagaca aaaggaggaa tccagtttga cctttgtagt gaaaaaaatg
     5461 tgctacttag atagccgagg gcgaggagaa gctagtgtcg agtttgctaa acaccacacg
     5521 actaaagaag aggctgacct tgttgcaagt gtttgtggcg gcgaagtagt cgaagttatt
     5581 aaacccaaaa gacgattttc aaaacctaag acaaagccag tgaaaaaaga gtgttgttgt
     5641 ccgaaaagta accaagcatg gatgagaggt gcaaaatgac ttgtttaaaa tgcaacgatg
     5701 aaacagttat ttggtataag acatcgcttg gatggtcaac ttgtgaacct tgtccaatat
     5761 gcaatgaaaa tggacgacgt tcgaaagaac gactcgaaag actaaagaag gagtatagca
     5821 aatggcaaca agaagcaaat acggaaacaa aaagcacgaa gtagacggca tcacttttga
     5881 ttctatcgca gaagcagagt actacgatct agccttgtgg caagcggaag cgaatggctg
     5941 gaaagtgaaa cttcaggaac gatttgagct gatgccgaaa tttgaactag aaggaaagaa
     6001 gtatcgcaag atcgagtata ttcctgactt cacattttat aaaaacggca aacttgtcaa
     6061 agtcgtagat gtcaaaggaa tgcagacaaa agactttaag atcaaggcaa agttgttttg
     6121 tcataaatat caagttccgt tgattttagc taaaaaatat cggaatacgt tcaaggaaga
     6181 gcgtttttaa cgaggtggtc catcatgaca acagaagaag tgattcaaat gcatattcga
     6241 agccttcagc gtgagattga cgatctggaa cgaacaaagg cagtgatggt caatgaaacg
     6301 gcgagaaagg caatcgattt gcacatagaa aatttaagaa gggaaattcg tagattggag
     6361 gaatgagcgt ggataagaaa gcagcaatga aacgaatcat tgaactgaca cattctgaga
//
LOCUS       DAXWLF010000144         6388 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXWLF010000144 DAXWLF010000000
VERSION     DAXWLF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.146"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            115..453
                     /locus_tag="ACGE4U_002243"
     CDS             115..453
                     /locus_tag="ACGE4U_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394361.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            563..784
                     /locus_tag="ACGE4U_002244"
     CDS             563..784
                     /locus_tag="ACGE4U_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394362.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            804..2960
                     /locus_tag="ACGE4U_002245"
     CDS             804..2960
                     /locus_tag="ACGE4U_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394363.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            2972..3205
                     /locus_tag="ACGE4U_002246"
     CDS             2972..3205
                     /locus_tag="ACGE4U_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8394364.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            3195..4061
                     /locus_tag="ACGE4U_002247"
     CDS             3195..4061
                     /locus_tag="ACGE4U_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394365.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            4076..6046
                     /locus_tag="ACGE4U_002248"
     CDS             4076..6046
                     /locus_tag="ACGE4U_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394366.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
BASE COUNT         2457 a          876 c         1249 g         1806 t
ORIGIN      
        1 aaagatgtaa atatcgagac gtctttttct tagttagatc attgacaagt gaacaaataa
       61 gatttgtcga tgatataatt aacttattaa aaatattaaa aaggaggttt ttatatgttc
      121 gattctatta aaaatttggt tagcggaatg acgcctgata tgaaaacttt agtacaagtt
      181 gttttgtatt tcttggcatt cgttttttcg ttgaaaccag gattcactgc aatgaaattt
      241 tttggtgatg caaaatgggg agatggatta aaatatgttg gggcgattat tgcaatcttt
      301 attattcctg taattacaac cgttatgctt tacacaatgg gtactaaaac aggtaatgat
      361 ttgaaccaaa aagctaatat catatcagca cttattccag ttgttggtat ctatctagtt
      421 tcaaaatttc caaaaaatga actgttaggt taaaagtgaa ttgaacaaat ttggataata
      481 tgccgatttg ttcaatttac tttttaccaa aaaactacgc tccaagcgta gtttagaagt
      541 gagggaagaa agtaaatgta agatggtttt tgatagagaa aaaatgttag ctcacgccaa
      601 cgaagtatta atgagcagtc tgaaaggtac cgaactggca aagataatga atatgaatgt
      661 taaccagttc tatgattatc gaaatgggag taaaaagatt gaaaaagcca gacttgaaac
      721 gcttattaaa ttcgaaaaag catatgtata tatgctagat aaacagaaaa gaactattga
      781 ctgaaaaaag gagttttaca ataatggcaa aattcaaaga tattaccgaa tctattaatt
      841 atgtggcaga aacagttcct gtagttgcac tctctttgaa gccacaatta ctcgaagtgt
      901 acaaaaaaga agaaaaagaa gcgaaggcaa aaaaagctaa agcaaaggta tggaaagaat
      961 tcgatgcgtt gctgaccaaa gatttaccaa agaaaaatta tatggtaatt gaagatgtac
     1021 aaaagctcaa cagtgaggga gttatagatg aggatgatgc tattctcgat tctgagaagt
     1081 atgtcgttct attgaagtca agtttgaatt atgtagctga tctaccagaa aaatttaaag
     1141 agaagctatt agcaggtgtc cgttttatgg atagtagtgg tcaattatta gttattccgg
     1201 atgatcctaa tgtgtttgaa tcagaagttg aagaagctac agacttctat agcgcagtag
     1261 agcaattaga agtagctact aatggattag aacgaattgc tgagaccttc aactcttctg
     1321 aagaagttga atttgaagcc gtagcagaaa atgtaccaga tgaaaaagaa gctctttttg
     1381 tggatgataa caaaccaacc gaagcagaaa accaagaagt aagcatagaa gaaacacaaa
     1441 tacaaacaga aacctaccaa caaccaaaaa ttgatgaaaa acccgaatcc caaagtgaca
     1501 aagaaaatta tgtttatgaa gctgaaaaaa tcacatctga aagtgatgta ccgaaaaact
     1561 tagatgctga ttttttagaa aaaaattttg aacagatcat tgctgacaaa tttcaacctg
     1621 tagttttaga tgagattaat gaccctgtcg atttaaacga ggagatgcta tccatacctg
     1681 agttgaaacc attgctgttt ttagctaaag agagtatgaa cgctcgcatc tctattgcta
     1741 ataataaaat caacagtgaa ttaaataaga cgaaggagca attgatcctc aaaggaaagc
     1801 aaatgttagc tgatgagctt ttgcggatag aggaagcaac ctcattaaat ggtcaaaatc
     1861 agtttacaaa tacactatcg gttgctaaag agagctacga agcagaagca tctaaaattg
     1921 atgatttagt gatggaaaaa tccaatatca ttcgtaaaga ttatgatatg gcgaaacagg
     1981 cttatttgga tgctgaacta aagaaactag agcaacggta tgatgctgag catttaccag
     2041 aagttaaaca acaagaagat gaatatagaa aagcaactga agaaagtctt gatcaacagt
     2101 accaagaagc aattgaatct attaaaaacg atgcattgaa acaaagccaa gaagcaaaag
     2161 aagttgtggt ggaacgaatt atcaattcat tgaaaccaga aattgaaaaa tcattaacag
     2221 acgtgcaaga aaaaacttta gagattgtcg aaaaaacaaa gtcagaaaat gaagctgagt
     2281 tccatgatac aaaagagaaa acaacagatg tggttgtcaa atattttgcg aacaaagaag
     2341 aagttgaaaa gcaggtagct aacaacacga aagaattaga aacaaaactt gtagagcaac
     2401 aaaaaactat cgaagaatat aaagatcgaa tgaaagaggt caaagattta gcagagggtt
     2461 ataaattaaa tagcgaaaag ttggcagaac aaagtgaagc tttaagagaa gacttatctc
     2521 gttcccgaag tgatctagct caaatggcag aaaaaacgaa atcattagca gttgctacag
     2581 aaaatcaatc aagttcaaaa cgtgatttta caagtaaaaa gatggctact atcatttctg
     2641 tcgctgcaat ggcttgcgga acgatcttgg gatcgtccta ctttattagt cataataatc
     2701 aacaagcaca ggctcaaact caaacatcta tttcgagagt ggaaaattcg attgggacaa
     2761 atacgaactc tgcttcaaca aatagtagct atgaagcaac accaactaca gagttcaaag
     2821 ttgggaaaga tgttgtcatc agtattgatg ggaaaacaac taaggctgaa gtgatttcta
     2881 ttgatggaga aaaagcagtt gtgaaaactc cagacggaaa tcagtacaaa attcaagctc
     2941 acttatctga taataactaa ggagggctat tatgcgagaa gaaaaaatag tttatccagt
     3001 aattcaaaac aatcaagaaa acatgacctc ttctcagcac ttacaggaag cacagctcgt
     3061 gtatcaggta aatgaactac cacaaacgaa cgagacaatg agcaagtcaa tatctttaat
     3121 tggcttgctt attgttgcaa tagtgataac tgtaggtgtt taccgtaagt tgaacgtgaa
     3181 aggaagtatt tctgatggca gataactcaa actatggatt taaacataac ttacatcaaa
     3241 aaccttctga aacaattgtc agagttgaat ggcttgaaaa gcatgtcaat agcacctatt
     3301 ctgttacagc aaaaagatta attgtatcag ctatagttgt tgcagtcgtt gttcttggat
     3361 caattgttta cttagtttct gatacgatta aactgaaatt tatttttgtt ccaacgttat
     3421 taatgataat tggatttctt attctaatta gccagtatat gtttattgat ccatctagtg
     3481 gttcattgat ttctggttct attagactgt tcaaatcagt agttaattca gcaagagtat
     3541 ctatggggaa aaagaaacca tttcgaggca cagagatttt aaaagtgaaa caagatggaa
     3601 caatcgtata tcgagatggg agtgtagcat taatgttcaa tctagatggg gctacatcac
     3661 taaccgcttt tccagaagaa gttatggctc aggaatcgaa atcaatacaa tatcaaaata
     3721 ccagagatcg ttccttagct gaaatcaaaa tcacttcatc tcaacctcag aatacggaaa
     3781 gacaaactcg aaatatgaaa atacaaaaac gaaatgcaag gagtaacaaa gcagtctttg
     3841 acataataga tcagcaagaa cgatttgtat cagaatatat tgatggtgtg aaaacaacag
     3901 taatccaata tcttatcttg aaatctcaat ctaagatggt attggatgaa ggtgtggaac
     3961 gtttagaaaa ctatacgagt agaggacttt attatagtgt cactcagttg tcagctaaac
     4021 aaacaaaaga aatattatct gatatttatg cactgaaata gaggtgagag attaaatgga
     4081 aattatgggt agatcacaac tattaaattt caaaaaaaag agaaaaacta aaaaagaaca
     4141 aaaaactaga aattcaaagg tagtgaataa tacctttgtt agtgatttaa catctttagt
     4201 tggttatcaa tttacaccat cttacattag gacaggtaag cggtggggaa caattgtgaa
     4261 attagtgaat acttatggaa tgaatagaaa ttatggattt ggttggatgg ttaaagctat
     4321 tcctgaagtg agagtagaag gggtaaaagc ctatctaatt gaagcagata agccagtgtc
     4381 acaaaaagat caacaagaaa taatgcacga gaatgttggg ggatcaacga atagttatga
     4441 aaataattta gaaggaaaaa attcagatgc tgaaattagc aaaactgtta tgcggattat
     4501 ggatttgaat acagcatctt tactagacag catggcaaat aaaattattg attggaagct
     4561 agaaatccta ttagtatccg attctcctaa taaaatcagc gaacagctaa ttaaactcca
     4621 acggttgtat gatgaaaaaa tgtatggatt aaagcttatg tcatatggag gagatcaacg
     4681 tgatctgttt gatagacttt taagtgaacc aatcgggaat gtctatgagt atacgtctat
     4741 gagttcggat tttgcaggaa atgatcatgc aattcgcaaa ggtttagatg atgcgaatgg
     4801 atgggctatc ggcactatgt ctactagcta cgcaagtggt gtagctttta tggcactgga
     4861 tgattctttt acaagtaaag ttttagtggc atcacacaaa aacgctacaa ttaaaggcta
     4921 tgatgaaaaa atctcagcat catcactttg ggggcaattg attgctaata atgcaatggc
     4981 aaatcataaa cgaacttttc atattgtctt aaataaattt gattactttg gtgatccaac
     5041 tggtagaaca tttgttgcca aaccttcgtt aaacagtgta atagaaaagg ttaatttggc
     5101 tgaagaaggt cttaactctt tggagctttt tggtaatcaa aaggacattg gtgatttata
     5161 tagacggaat ctcgataaac taactcagat tttttatttg ttagcaggca gaacattaga
     5221 ttctgcaact agagttttat tgaaaaaaga attaggtaac ttttatataa gtcagaaact
     5281 ttgggatatt aattatgaag aacgttcaaa acttttggtg aacagtagtg atacaatgcc
     5341 agttcttggg gattttatta gctatttgac taatgctgaa acaaaagcca aaaaatctgg
     5401 tactgaaaaa gaagtcgatc gtattattgt attaaaagac acattgagaa cagcattaga
     5461 cagcttctct aggatgttta atcaaagaac gacactgaaa gaaatcggta aaagtgacaa
     5521 attacagttt tactataact taggtgatct aagagcagaa accaatgtta tggaagctca
     5581 atttttaaat acctttgatt tcatcacatc acaagcaaaa aaagatgata ttgttatgat
     5641 tcacggcttc gataagttat ctattgaaac cgttgggcta attaaaaaaa gattaacaat
     5701 gcttgaagaa gatggaattc gtcttgcctt tctatttgat acaatcggtt caggagaaga
     5761 caatgaatac aaagaagata ttgaaacagc tgatatattc aatacagatg gaaaaattta
     5821 ccaaagtttt gaaagtgatt ttaattacac tatcattgga aagatgacca gtggagagtt
     5881 aatgaagtat gaaaaaaaag ttgctcctgc tagaaaagga gaagatgatc aaaaactttc
     5941 tgttgatttg agacagtatt tgacaacagg aagaagtaat aaatttcaga tcaggagacc
     6001 agtggatctg actaataatt ttgttattgg agagtttatt ctttaagttt tatctttgat
     6061 tttgagtgta taaggaaagg ataagaaaaa acatgtatat ttgagactaa aaacagtctt
     6121 agagaacatg gtacgttttg tgaaaagaga actatagaaa tttcataaag tgaaacctta
     6181 ctgagtgaaa taattatagt ggaggttaaa taatgaaacc aaacataaaa attttagatc
     6241 gaatatttct aggtagagac acagaagtaa tcctaattca acatgaagaa ggatttgaag
     6301 ttagtatagg tattcagaaa cttcaaaaac ctcattattg taatcagttg tataaaaact
     6361 ttactgatga agaaaaagcg agagtttt
//
LOCUS       DAXWLF010000145         6289 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXWLF010000145 DAXWLF010000000
VERSION     DAXWLF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.147"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(31..633)
                     /gene="nrdG"
                     /locus_tag="ACGE4U_002249"
     CDS             complement(31..633)
                     /gene="nrdG"
                     /locus_tag="ACGE4U_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFF8394367.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(948..3137)
                     /gene="nrdD"
                     /locus_tag="ACGE4U_002250"
     CDS             complement(948..3137)
                     /gene="nrdD"
                     /locus_tag="ACGE4U_002250"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFF8394368.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            3463..4230
                     /locus_tag="ACGE4U_002251"
     CDS             3463..4230
                     /locus_tag="ACGE4U_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8394369.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            4220..6262
                     /locus_tag="ACGE4U_002252"
     CDS             4220..6262
                     /locus_tag="ACGE4U_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8394370.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         1860 a         1120 c         1211 g         2098 t
ORIGIN      
        1 ggaaatccgt tttctctccc aaagctgttt ttatatcaag tcttgctttt tgatttgttc
       61 ataggtttct gttgcatccg tacatttttc ccaaatcaca acttgattgg attctaaaga
      121 tttttggacg tcaatgatcc gctggttgct acttccgcga aattgtagtt ttaggtcacg
      181 tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga gttctaattt
      241 atcttctgaa tcttgtagca attcatcaaa ggtatagcct gtccatgacc agatatcttt
      301 tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc agacaaacat ctgtattcaa
      361 aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca agtcagcgat
      421 gatttgttct tctaattcag gtgtgaatgg tttgccatat cggaaatttt gtgcagcttt
      481 gttgaagcag ccttcgcagg caaataagca gccactaacg taaagactgt tgcggacacc
      541 ttcaccatcg acaaaattaa acggtttgta gtcagcaata tagttttggc tgtagtcttt
      601 tgctaaccat tcttttggtt ttggatttct cataaaaaaa cttctttcat ttaaggttca
      661 aagatatagc tattgaagat aaaatctact cgaccacata aataagaaca aactgaccta
      721 aatccattgt tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa gatacaaagc
      781 catcgaaaga gggtggtgaa aaaagacttg tcatagcctc ttattctgaa taaacggtgt
      841 tcaaaagctt gcatctgcag taataagccc aactaaacga aaaatatgag gaactatttt
      901 tgtttagcta ttcttattac ttgatgcagg acagcttttt cacaacctca tttcatgtgt
      961 ttcacacgtg aggaaatttc tttatggcgg ccatgtacca tcggacgagc ttgcggattt
     1021 cctaaatagc cacaagtacg tttgactaca tcacatgttt ttggatcatg atttccacat
     1081 tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc acattgatag
     1141 caatgatcga ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc aaaatcccat
     1201 acagcttcta aagctttcgg attttgttgg agcatagggt attcacagta gtggatgaat
     1261 ccacctgaac aatattttgg ataatccttc tcaaattcca atttttcaaa tggcgttggg
     1321 tttttacgca catcatagtg gaaactgttt gtataatatt ctttatctgt gatattttcg
     1381 atgatgccga atttttctgt atccaaacgg cagaaacggt cagttagact ttcacttgga
     1441 gttgaataga cactgaaatg ataaccatat tcatcccccc atgcatctgc attagctttt
     1501 aattctttga cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc ccattcgcca
     1561 ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac agtagctcga
     1621 cggtttttga ataattcatt gacagaatcg gtacgtgaaa gacgtttccc gaaagcacca
     1681 tacatatata aaattggcgc atttgctgga atggcttctt tacaacgttc tacacgatag
     1741 agaagcgcat ctttcatgat ttctaagcgg tcagacagta actgccagaa tttattctga
     1801 tctccttttg attcgatcgc gattcttggc aaattcaatg taacaacacc taaattcatt
     1861 cgtccgacat ttacttcttg accattttcg tctttccacc cttgcaagaa tgaacgacag
     1921 cccataggta ctttgaaact tccagttaat tcaacgattt tatcgtaatt cagaatatca
     1981 ggatacattc ttttcgttgc acattccaat gctagttgtt tgatgtcgta attaggatct
     2041 tcagggttta agttcacacc ttttttcaaa gtgaagatca gtttagggaa gatagctgtc
     2101 cgcttttcac tccctaatcc attgattcga atctgtaaaa ttgctttttg aatttctctt
     2161 tcaaaccagt tttctcctag tccaaatcct aatgaagtga aaggagtttg tccatttgaa
     2221 gtaaataagg tattgatttc atattccaaa ctttgcatag catcgaagat atcttttttg
     2281 gtttttgctt ttgcatattc tttttggcgt tctggcgaat cgatccattc ttccgcgtct
     2341 tttagatgtt ttttatagtt caattctgca aaaggagcaa gcaattcatc tgttcggtcg
     2401 gcagaacagc caccatattg acttgacgct acattggcaa taatctgtga gatttgtgct
     2461 gtggcggtct ggatagattt tggcgattcg acttcggcat tgccgatttt gaacccattg
     2521 ttcagcatgc ctttgaaatc aatcaagcag caattcgtca tcggtgtata aggatgataa
     2581 tccaaatcat ggtaatggat atcgcctttt tgatgcgcat ttgctatatg tggtggcaac
     2641 attttcagtc cgattgattt tccaacgatc ccggctgtca gatctcgctg tgtattgaaa
     2701 acattactgt ctttatttgc gttttcgttc acgacagttt ggtccttgtt gattagcttt
     2761 tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa aatctcgttt ggtacgataa
     2821 ttgatgtact cttctgctaa ttcatattcg tttttttcta gtaatatatg ttccacgatg
     2881 ttttgaatct catagatttt gacattttca gcaaaccggc ttttgatttc ttggacaacc
     2941 aagtctgcaa tcatttgaat tttttcataa gtaagtggtg ataatgagcc gtgaattttc
     3001 ttttctgctt tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac atggcggcca
     3061 tcgcgtttga tgactttcat ctgggatgtc tctggctgct gagtaacgac agttttttct
     3121 tcataattta ctttcattat cttcaactcc tgctaaattg ttcttgtcta tcataaaaca
     3181 aaatcgggga aggagcaact aaatatcggc tatatatagt attcaatgtg ctgtataatt
     3241 ggatttctat actatatgta gtgtatattt tattgtgaaa aaaggtgaaa acttgtagct
     3301 ggtggatttg ttttttgcat atttaatttt gctgaataaa ataattgaat aaaatttttt
     3361 attcagtcca agtaaaagtg acaaaaacat aagagcaaat tattcgtaga tatgctacta
     3421 tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa gatgatcgaa
     3481 gtgaaacatc taagtaaaag ctacggcagc aaactttctc ccgtcaaagc cttagataat
     3541 atctcattca cagtagaaga aggcgagttt gttgggatta tgggcccaag tggtgcagga
     3601 aagacaacgc tgatgaatat cttgtccacg atcaatttgc ctacgatggg cagtgtctcg
     3661 atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt tcgtcgaaaa
     3721 aaactaggat tcatttttca agagttcaat ttgatcgata ccctaaatgc aaaagacaat
     3781 atcctattgc ccctagcagt tgaacggatg actaaagagg aaatggaaaa gagagtaaga
     3841 catgtagctc aattgttgaa tatagaagaa ctgctcaaac ggtatcccga tgaactttct
     3901 gtcggacaga gacaacgaat tgcagctgct agagcacttg ttgttcagcc acaagtgatt
     3961 tttgcagatg aaccaactgg ctctttggac tcgaagtcgg ctacagaatt gttgaactac
     4021 ttgaagacca tgaatcaaaa agaaaaagca acgatactgt tagtcaccca tgatccatat
     4081 acagcaagct actgcgatag aattttattt attaaagatg gtgtgatttt ttctgaagtt
     4141 gtcagaagag ggacaagaag agagttcttt gagaaagtaa tagacatgca ggcgacgatt
     4201 ggcggaggag gaaaaatgaa tgctgtataa actagccgtt aaaagttttt ttcggcagtc
     4261 acgaggctat cttgtttatt ttttcagttt gactttatcc gtaatgatct attattcctt
     4321 cagcgcaatg acctacgatc aatctttagt cagaagagca agtcaagata cttcgataga
     4381 tggtggattg agttttggtg gatttatcat tactgttgtt ttgctttttt tcgtattcag
     4441 tgccaaccga tttttcttga atagaaggca aaaggaaata ggcatctatc agctttttgg
     4501 tatgaataaa ctgcagatat caggtatata tgtgttagaa atcatgataa ttggattgtt
     4561 cgcctgtatt tctgggatat tactcggtat tattttttcc aaactctttt caatgatttt
     4621 ggttcgaatg atggatatgg atcttactag tccatttttt atctccattc catcagtagt
     4681 agatacttta tttgcttttt tcatcatcct tttagcagta tctgtatatt caatatggaa
     4741 aatatggcgc tatccaatga ttcgaagttt tggagaaaaa gaacaagcag atagttcaac
     4801 aatgcgcttc aggacaaggc accgattact cgcagtcatc gggttactac ttttaacgag
     4861 cggatatttt ggagctagtc attttaggga aatcacttca tggatgatca gttatcatta
     4921 tcttgataca ttagttttct tttttccttt tttaattttt ttcgtttgtg tggtcggtac
     4981 ttatttgttt ttctgttata catttcggtt atttttgatc atattgagta aatggaagat
     5041 ttattactat ggtatcaatt ttttgataat gggtaacacg caagttcatt tgctcaaaag
     5101 ttggcgtaca aattctctga ttacagtcat tctcggaatt tctttaatga caattggcgg
     5161 cacggctagt atctctacta ttttatccta ccaggagaaa gtttcctccc cgatggacta
     5221 tcagtttgat gttgagacag agaaaaaaat caagccgatt ctagcagagg aagggcagaa
     5281 gataactaaa gaaatcacct tgcattataa agtcattggg agtatctata gtcaaaatct
     5341 cgaagaacct atgtttcaaa ctgctaatct gatcacggaa acacaatatc aagccttcag
     5401 aaaagttaat ccagatttgc cgaacatttc tttgaaatca cctggtcata ctgttttgct
     5461 tgatcaaatg caaactcttt ttcggaatat ttcattatat ggatctagta ttggactgcc
     5521 agataatcaa gaattgaaga ttcaacaaat gcttccaagt gtattaggtg acgaaacaat
     5581 ggtatatcta agtcctgtac tgattgtaga tgataccgtt tttgaaaaag caaaaggtgt
     5641 ggattatcaa gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa tagatgaacg
     5701 actgagcaat gacctgtcga taaagtggct caatgctgtt tattattctt atgatatcgt
     5761 aaatggaagt ctacaaggag aaattagcac aaagaaactt ccggatgacc aaatgaagga
     5821 agcacaattt agccaagaaa gctctcgttt gaatttctct tccagctatt cgaaattgcg
     5881 tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg ggatgatcgt
     5941 gattattgct actggtagta ttttgacagt ccggcaatta gcagaagcag aggaagaaaa
     6001 agaaaattat cacctattga ctaagctggg gattccacaa agaaggatca gacggatgat
     6061 cttcgagcaa aacgcactta ccttctttcc acccatgata ttagggatca ctcacgcagt
     6121 atttgcaatc aatgtcttca ctcagtatat aaaaactgcc gattactggt tggcttatct
     6181 tgtgagtggc ttattgatcc ttatctatct tttgctttat gtcattacga gtcagttata
     6241 ttgtcggatt atcgaggaat gatatacgga ataagacaag ataataaat
//
LOCUS       DAXWLF010000146         6257 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXWLF010000146 DAXWLF010000000
VERSION     DAXWLF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.148"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            16..873
                     /locus_tag="ACGE4U_002253"
     CDS             16..873
                     /locus_tag="ACGE4U_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8394371.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            893..1453
                     /locus_tag="ACGE4U_002254"
     CDS             893..1453
                     /locus_tag="ACGE4U_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFF8394372.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2342..3109
                     /locus_tag="ACGE4U_002255"
     CDS             2342..3109
                     /locus_tag="ACGE4U_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFF8394373.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3135..3680
                     /locus_tag="ACGE4U_002256"
     CDS             3135..3680
                     /locus_tag="ACGE4U_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394374.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYVHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3681..4247
                     /locus_tag="ACGE4U_002257"
     CDS             3681..4247
                     /locus_tag="ACGE4U_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394375.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            4262..4510
                     /locus_tag="ACGE4U_002258"
     CDS             4262..4510
                     /locus_tag="ACGE4U_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394376.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANGFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4530..4769
                     /locus_tag="ACGE4U_002259"
     CDS             4530..4769
                     /locus_tag="ACGE4U_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394377.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            5140..5421
                     /locus_tag="ACGE4U_002260"
     CDS             5140..5421
                     /locus_tag="ACGE4U_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFF8394378.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            5411..5752
                     /locus_tag="ACGE4U_002261"
     CDS             5411..5752
                     /locus_tag="ACGE4U_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8394379.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKTGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
BASE COUNT         2345 a          844 c         1262 g         1806 t
ORIGIN      
        1 tagatatcat aatttatgga agaaaaatta attaaaaagg atgagattat aatggggtta
       61 ctggttccga gagctaatac ttatactata cctttaacga aacgccaatt aattgctaaa
      121 agatatcaaa ggattactag agccatcaat agagagtttt ggaattctga aagtgatact
      181 gctcacagtt tatacgtagg ttcttatggg agaggaacgg caattagtac tagtgatata
      241 gatataatag ttgaattacc gatggcagaa tttgacagat tcaaaaatta tttatcaaat
      301 ggcccgtcta agttattaca agttataaaa aacgcatttc aggaaatact accaaatagt
      361 gatattagag ctgatggaca agttgttaaa ataaatttcc atgatggaat caaatttgaa
      421 atagttccag cttttaatga aaaagattat tggggtgagt caaaagggtt tatttatcca
      481 gattcaaaca tgggagggaa ctggaaagct acaaatccca aaaaagagca agaagccatg
      541 aaattaaaga atacaaaaag taataattta ttatatgcta cgtgtaaaca ttttcgacat
      601 gtacgagata cagaatttac gagttatcat ctttcaggaa tagtaattga cagtttcgta
      661 tatgaggcta tgggaaattg gaaattcgtt gaaaataaca gtgggggtca aaacattagt
      721 tctgtatctt atgagacagc tttattagaa tactataact cacataaagt tatgggcggt
      781 ttaaatttat actctcctgg aagcaatcaa tttgttaatt ctgatagtag tatcatatgt
      841 ttagaaaaag tattaaaaaa aatagcttta taaagtttta tggaggaaca acatgggaga
      901 cgaaaaaaag cgcttggaag ttcaattacg tgaagcatat ggacgagtaa cgtatactta
      961 caccactcat ttaaagcaag ccgataatat tatttctgac aataaaattt taaggtgtct
     1021 tcaaattggt ttgtcagccc ttactactgg aggggtactt agtaccatta ttactgataa
     1081 tagtgagtta acctttgttg ctggattatg ttcaacaatt tctcttggat taaacttata
     1141 ctttaagaat tttgatcttg ctgaagaagc taaaaatcat caaattgttg ccgataaatt
     1201 atggttgatc agagaagaat acgtaagctt actaacagat ttaaatcgtt taagtgttga
     1261 agatattgtg aaaaaaagag atcaattaat gaacgaaact catgaagcat ataaaaatgc
     1321 ccctaaaaca aatagtaaaa gctatgaatc agctcagaaa gcattaaaat ctgaagaaga
     1381 gcaatttttt acagaagagg aattggataa gatgttacca gagcatctta gaaaaaagag
     1441 ttctaaatat tgaactcttt ttttatgctt aaaattctct tgtaattttt ctattcatct
     1501 atatttaaga agaaatatga tgatagaaac atctcctaaa gccaccgcaa gccgttctaa
     1561 gcatagctta gcccgacatt gctagatatg ctcatctcac catgaacgtt tcctaaagcc
     1621 accgcaagcc gtcctagcca ttgcctagtc cgacatcgct agattagtgc atctcgccat
     1681 aaaagttttc taaagccacc gcaagccgtt ctaagcatag cttagcccga cattgctaga
     1741 tatgctcatc tcaccatgaa cgtttcctaa agccaccgca aaccgtccta gccatcgcct
     1801 agtccgattt tgcttgatcg gttccatttc tgtttattta gtgggtcaac accgtaatct
     1861 taaatagcca tcgcctattt aagattacta ggtctgcttt gaaggtaaaa agtctttcat
     1921 tcttcaagac ttttaggctt tcaagctacg cctgaaagca ttgaaattca tcgctcttta
     1981 tttggttttg ccatttataa ttccttacgc ccattatctt tttcgcctgt cataccttgt
     2041 cagaagtgtt tagagcttat ctcatctttg gattaataca agtggcgtaa atttttttct
     2101 aaacgcaagc gttacgaaaa taatttactt aaacacttgt attaatccgg cagataagaa
     2161 cgcattctaa tcactcccga caaggcaaga caggcgaaaa agataaataa atgaacgaaa
     2221 ggaagaaaca taaaatgcaa aaccaaatag cgatgaagaa aaaaccacct ccctctaaaa
     2281 caatccaaac aaaaaagaat tttgaaaaac caaaaatcaa aaacagaaag aagcgttcaa
     2341 catgagagat agaattttag aagttaatca agaaatggta aacaagttaa caaatatcag
     2401 tgggaactat gaaaagtacg atggtacttt acttgataac tatatttttc acgatacaca
     2461 aaatatcaga attggcagaa gtaaaccgag agcgttcatt ctcatcaaag agaaatattt
     2521 gaatgaatgg aatagcacct atgaaatggt tatgactgat aactttgaaa aggtattgga
     2581 tttcagaaat gctttgttgg gttctgatat gggaaatagc agtcttgaat ctattgaagt
     2641 tgaaaacaag accgagaatg aaggtgaagg attgattaag aatgtcttga aaatcgaaca
     2701 aaaatatatc agtgaagata gaagttttta tagagtcaca agctcggttg gtctagcaaa
     2761 ctttgctact aacttaattg gaaacaatgc tagtgaatct ttgctagtga tgtgtctgaa
     2821 tacaaagaat gaggtcattg cttatagcga agttttcaaa ggtggaatta atcaatcagt
     2881 agcgatacca agagagattt ttcaactagc tttgctaaac aattcagcaa aaatcgcagt
     2941 atcacataat catccgagcg gatatgaatt tccaagtgaa aatgacaaaa gttttactac
     3001 gaatttgaaa aacgcaggaa aaacaattgg tattgaattg ttagaccata tcatcgtaac
     3061 aagtaaaaac acaagctatt actcatttca agaaaatggt tctttataaa aattagagaa
     3121 aagaggaaaa caagatgggc gaaagaacac aagcgttaat tacagtcaaa tatggtaaca
     3181 gcaaaacgaa agcaagcgtg tcgattcatt atcagtgggg gtatggtcga tatatgctga
     3241 tggacgtatt gaatcaagga atttatttaa tcaataaagc atacgactta cgagataaat
     3301 caagagaaga attagcaaaa ggaatctttg atagaagtac aggagttatc aattttgtta
     3361 cctatgaatc tggagtgagt gcttatgatg aaacagggtt tggtgggcgc tatgaacctt
     3421 atcaagaaac gattgaagat gtgatgaagt tttcagataa caatgatggc tatgttcatt
     3481 tagagatggt gttcgatgaa aatggattta gtctagatga taaaaccaga ttgcgattgt
     3541 ttgattttga gggtcaacag ataggactaa acaagttttg tgaaagcgga aaagagtttg
     3601 ctacgaaaga ttttattaag ggatacagat tattgctaga aaattatgga ataaacctag
     3661 aagaagagag cgtgaaataa gtgtttgaaa aaagaaaaga ccagttccct aatttagaag
     3721 cgaaaagtca aaaagtgatt gatggtgaaa tgattgaatt attccaaaaa agttatggaa
     3781 ccatagaaac taccgcaagt tgcaaatact actttcaagg aaagagaaac acgtttaaag
     3841 gctttgccga ttctgatgaa gaattggcaa agaaaatcat tgattggctt gtcagcagtg
     3901 aacacgtagc aagggaagat accgttagaa caacgattaa tggggaagtc ctaacaggta
     3961 ctagatgtgt ttgttgtaat catgaatttg tagaagatga aggttgctac cgaatcaatg
     4021 aatatggtga ttttatttgc cagagttgcc acgagataga atttccaacc gaaagaagtt
     4081 ggtttgaata catgctacaa gattatgaga tggaaaaaga aaatggtgaa gtagtttttt
     4141 gtaccaaaga ggaattgagt aagagaagcg atgatgaaat tgaagattgg tgtgtgtggt
     4201 tagtaggggc agatgattgc gttgcatatt acacaacagc agaataggag tgtgaatcag
     4261 catggaagga atggattttc tagatcatga agatttggtt gattttggtt atacatggaa
     4321 aggaatggta ggtattagca gaagtcttgc taatggcttt tacgaaagaa attatgcagt
     4381 atatgttctt tatgatgacg atacagaaag ccttgttgac gaggaatata aattggattt
     4441 agagaatgtt ttatatggaa tagaaaagga agatttagca aaatatattt tttcatggtt
     4501 gggtcaataa gtgaaaggaa gatagaaaca tgacaaaatt taaaagtaag ggtatgcaat
     4561 ggtgttctta ttattatgtt ggaaatgatg aagatttatt gagaaaaacc caacgaaaag
     4621 gttatccaga taagatattg aaacaaatag gttattacat gccagaagag aggatagaaa
     4681 aagaattttg tgaggttctt tttgatagtg aacagctatt agttttgtta acccaaaata
     4741 aattgatttg ccaatttgaa aataattaag atgaggtggt gaaccatgaa tgaagataag
     4801 atggtattaa gtgaatatgc tagattgaga actattaatt ctttagcatc tcatttaagt
     4861 tgttctttcc taacaaaggt gctaaaatta atttacaaat gtgaagattt tgtacaagag
     4921 aagcacaaag acaagcctac tgcgaatagg cttgtctttt tttcattcat tacgattccg
     4981 tatgtgtggg ttagcgacaa tttgttcaat taccgtatga tatagcatct taatttgatt
     5041 agattacttg caatgtagtt tattaagagt ataatataat cataaatgtg gacaatttga
     5101 taaataatga gtgttttata ctcaaaagga gtggatgaaa tgaaggtcaa aaatccaact
     5161 gaaaaaagca gaatccaagt cggaatagac aagaatttaa aagagaacgc agaaatgata
     5221 ttggaagagc taggtttaaa tcctacaaca gcaataacaa ttctttacaa acaagtagtc
     5281 gctagaggtg agtttccagt agagataaag ctaagtgaag aggagaagca aggaatcaga
     5341 ttgcaacagc taactaagga catgccagta gatgttctag atacagatga aaagttagaa
     5401 gagtggttcg atgaagccta atgagatagt aacggtatat gttgcattta cagataaaaa
     5461 tggtggtaaa agaagaccta ttttggtggt tagtgataaa gaagatagag tagaattctt
     5521 tggaatcaca agtcaatatg agaaaaaatc agaccaaata aagagggtgt attttcctat
     5581 tgcagaatgg gaaaaaacag gacttaaaaa gcaatcatgg attgacgtag ggtctttaaa
     5641 ggctattccc aaaagccgag agaacatatc attcaaaaag gttggtatgt tatcagctag
     5701 tgatacgaaa aagctaaata ggtttatcga gaaattacaa agaaaagtct aacaaggctt
     5761 ttcttttttg tttatatgta tattaagata tacgtttata caaacgtatt tatgtatatg
     5821 aaaatatacg taaatatata cgtgattata aacattaaga aaacgttaag aattgttaaa
     5881 taatgatatt tagtaatata cgaattataa tatatatttt atataaaatg tatgtaaata
     5941 aataaaatgt ctgacggata ttgaatagca ataaataaca ataattcaga cgaatacggt
     6001 gagtgaagta gcttgctacg aacgaacagg cagaatgaaa cggcagtttc attagagcaa
     6061 gtcgcttgcg acgtagcgtt accgctgatg taactggcaa gttacataga gagagtttgc
     6121 ttgcaaacga acgttaccta tttgaataga aataaaaaat aacagattag tgaattttga
     6181 ataggagcca caactcaacc aaccgcttgc ggtgggtgag tcccacggag gaaaagacga
     6241 acgaacggtc gcgtagc
//
LOCUS       DAXWLF010000147         6194 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXWLF010000147 DAXWLF010000000
VERSION     DAXWLF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.149"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(258..743)
                     /locus_tag="ACGE4U_002262"
     CDS             complement(258..743)
                     /locus_tag="ACGE4U_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394380.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(882..1097)
                     /locus_tag="ACGE4U_002263"
     CDS             complement(882..1097)
                     /locus_tag="ACGE4U_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFF8394381.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1321..2121
                     /locus_tag="ACGE4U_002264"
     CDS             1321..2121
                     /locus_tag="ACGE4U_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8394382.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2140..4008
                     /locus_tag="ACGE4U_002265"
     CDS             2140..4008
                     /locus_tag="ACGE4U_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8394383.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4001..4492
                     /locus_tag="ACGE4U_002266"
     CDS             4001..4492
                     /locus_tag="ACGE4U_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFF8394384.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4480..5034
                     /locus_tag="ACGE4U_002267"
     CDS             4480..5034
                     /locus_tag="ACGE4U_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFF8394385.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5052..6047
                     /locus_tag="ACGE4U_002268"
     CDS             5052..6047
                     /locus_tag="ACGE4U_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFF8394386.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2173 a          982 c         1243 g         1796 t
ORIGIN      
        1 tctatccaaa tatagaaaaa ttgaatctag aaaaccgccc gacaataaat ttcattaact
       61 cagttgaccg aaaatgaagt agataccaaa aatttatcaa gagagcaagt agagaagtga
      121 gtatattcat aagagaaata gctgtaacga attattaggt taatgcaaat gcttatttat
      181 actccgtata actttctcag aaaaaatgat accaataatg acttgctagt actgtaaatc
      241 cgtataatat catctaatca aaatggtgtt ccatttgcat ctgtcatcgt aatcgatcct
      301 tcgggtgaca ctcgataaaa accaacgctt ccagatcctc cttgggaacg gatttgtttg
      361 ctggaagctg tgaacaaata atctcgatca attttttgat aaaagctaat aacaatatcg
      421 tcactttgta attcggaacg gtgggcaata agaaatagaa gtgctttgtc agagttttct
      481 aatggaagag aaggtacagt gctttcttgc atttcagatg ttgaagaaat gctaaacgac
      541 tgaatacttg attggctatg ttgcgaatct acattaccag tagaagtatt agtaatggaa
      601 gtagtagaag tacttttagt tgaaccaacc tcagttgttg aaatggctgc tgaagattct
      661 ttaacagaat tctttgtttt atatccacag gcagaaaaaa gtattacagt tcccaacaag
      721 cctgcgtaaa tccctttatg catgatagct cctcctaaaa tagattatct ttattcagta
      781 tatcacttaa acaaagaaaa gcagatggtt taaaatgaaa aaaccttccc aataaaggga
      841 aggttcatga agataataac ttttttaata attatagctt tctaatatct ttcatcaact
      901 tttctagtaa tcattgggaa aaatttcaag cagtagatcg cacaaatacc tacgacgata
      961 taaatgatct tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac taaatcaagt
     1021 tcgaacaagc ctaccaacag ccagttcaac cctcccacaa tcaataaagt taatgcaata
     1081 gcatctaaag ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa
     1141 aagagaaaca ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa
     1201 attttttttg ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt
     1261 aatttttttt agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat
     1321 atgtcggatt ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga
     1381 attggaagaa gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat
     1441 gtaactgaat gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct
     1501 aaaacggaca tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt
     1561 ctagtcaata atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa
     1621 agcaagtatg aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt
     1681 gtatatttga tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc
     1741 gtgaatcttt cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct
     1801 acaaaagcag cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat
     1861 gttcgtgtgg taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa
     1921 tatgaagaag ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac
     1981 agtcgaacaa gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta
     2041 gtctgctatt atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca
     2101 ggaggtaaaa caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg
     2161 taccaattat tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa
     2221 gaaatcaaaa ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt
     2281 gccggtattg caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt
     2341 gatttttttg atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca
     2401 tccaaaagaa tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg
     2461 gatgatttgt ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca
     2521 ttaaaagaga tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta
     2581 agaataatag gagatgagct agaattacga ctacttttat tttattttgt ttctccctac
     2641 ttttcagaga cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag
     2701 aagaagacga atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct
     2761 ttgaatagaa tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt
     2821 tttgaatata tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta
     2881 actttgagcc aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt
     2941 ataacgatga taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg
     3001 cgtattcgac gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa
     3061 cagcacctct ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt
     3121 tatggtgaga tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa
     3181 gcattagaag ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg
     3241 tatttggagt tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt
     3301 gttcatacga caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg
     3361 gggagggata tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac
     3421 aaatatttcg ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg
     3481 atccatttga ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa
     3541 aaaaagcgat tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa
     3601 aagacgtatc aagaatattt aaacaaatta gtcgctcctt tgatcagagg ctattatgtg
     3661 gatgaagaat ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac atttgttaac
     3721 aaagaatttg cttttcctca cacaaccaac cagctatctc aacacattgt tttatcagta
     3781 gggctaataa ctgatcaagg tttagcagaa acaggaggaa atggagttat ttttttattt
     3841 gccatacctg aagaaatgac ggctgaagtt gagaatgaat tatttgagct atacgatatc
     3901 ctatttgaac tcataaaaat gggaatagat gagtttattg aattagcacc gacacaagaa
     3961 tcatttttaa aactaatcaa aagtaaagga gagagacttc atgaatgata tgtatctatt
     4021 catagttttc gcagcttcag cttatgtgct gcaagcagtt atcagttgga agcaactaaa
     4081 gcatttcaat caaacatata gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg
     4141 acctgggaaa atccgttcag gcacgattgt tatgctagct atagacgatc atgatcaagt
     4201 tttagaagtc agaaaaatgc aaggggttac atctttggca aaatttcatt cattacctgt
     4261 ctatgcgggt gaagatatcc attacatcga tcgttaccat ccctcagttc gagcggaaga
     4321 tcgtttgaca caagcagctt tagaaaatgc cagagaagta tacttacgaa tagaggcagg
     4381 aaatgaaaaa agtgaacaac cttcattgcc ttttattgct atgctcaata attttcggat
     4441 atggaaaagt acgatcaaag aaaaaaggag tgacaaacga tggatatcat aacaaaattt
     4501 gctgagggat ttatgaggct gttccaaaca ggtgcagaaa cattcataag ctggatgaca
     4561 ggaatcgtac cagttgtctt gatgctgatg gttgcaatga atacgctgat tgctttttta
     4621 ggtgaagacc gagtaacgaa agtagccaaa ttatcagcga aaaatccgct tactcgttac
     4681 cttgtgttac cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt tacaatggct
     4741 cgtttccttc cagaatacta caaaccaagc tattatgctg ctcaagctca attttgtcat
     4801 acgagcaatg gtgtattccc ccatattaat cctggagaat tgtttgtatg gatggggatt
     4861 gctcaaggga tcgaaacctt agggctaaat tcgatggagc tggctatccg atacttgctt
     4921 gtgggactgc tgatgaactt tattggtggt tggataactg attttactac tggatttgta
     4981 tgcagacagc aaggaatcgt tcttagcaaa aaagtagagc tttcagttga ttagagaaag
     5041 gagagtttta aatgtatggt gatattaaga tcgtaagagg aaacggcggg tatggaggtc
     5101 ctttgattat taggccgaca aaagataaac acaagttcat ctatgtcaca ggtggaggtg
     5161 agaggccaat gatcgtagat aaaatagaag aactgacagg tatggagtct atcaatgggt
     5221 tcaaaacatc aatacctgat agtgaaatcg cgcttgcaat cgttgactgt ggagggactc
     5281 ttagatgtgg aatttatcct aaaaaaggga taccaaccat caatatcgtt tccacaggaa
     5341 aaagtggccc attagcacaa tatatcactg aagatattta tgtttcagca gtagggttag
     5401 aacaaataca gccactaatg gaaactgatg ataacaaaga aacagtaaat actgcatctc
     5461 cagataaaaa gtacaaatat gatacttcta aaaagataac cgagcaaaaa gcagagcagc
     5521 aaaactggat agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat
     5581 cagcaagaga agcagtagat acgatgatcc ggacagtgat cccttttatg gctttcgttt
     5641 cccttttgat tggtgtgata caaggttctg ggatcggtaa tgtatttgct aacattatgg
     5701 cacctttagc aggaaatgca gttggattaa caatcattgg ctttatttgt tcactgccat
     5761 ttttatctcc tttgcttggt ccaggggcag ttattagcca agtgattgga acattgatcg
     5821 gtgtagagat tgggaaagga aatattcctc cccaattagc attaccggca ctatttgcta
     5881 tcaatactca aaatgcatgt gattttattc cagtcggtct aggtttggaa gaagcaaaag
     5941 ctgagacagt agaagtaggt gtaccatcag ttctttattc aagatttctt aacggcatcc
     6001 cacgagttat cattgcttgg gtagcaagtt ttggattata tagctgagtt tagtctaatg
     6061 gtgataagtc aagatgaaat ttaagatttt ctttattgag taatatgttt tgaagcgcac
     6121 gaaactagac ccgatcaaaa tcaaattttt ttcgggttga ttcaaggatt tgcgtcaata
     6181 acggattgtt attt
//
LOCUS       DAXWLF010000148         6187 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXWLF010000148 DAXWLF010000000
VERSION     DAXWLF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.150"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            282..971
                     /locus_tag="ACGE4U_002269"
     CDS             282..971
                     /locus_tag="ACGE4U_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8394387.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            958..2166
                     /locus_tag="ACGE4U_002270"
     CDS             958..2166
                     /locus_tag="ACGE4U_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFF8394388.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2460..3731
                     /gene="serS"
                     /locus_tag="ACGE4U_002271"
     CDS             2460..3731
                     /gene="serS"
                     /locus_tag="ACGE4U_002271"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFF8394389.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4280..5764)
                     /gene="guaB"
                     /locus_tag="ACGE4U_002272"
     CDS             complement(4280..5764)
                     /gene="guaB"
                     /locus_tag="ACGE4U_002272"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFF8394390.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1898 a         1168 c         1210 g         1911 t
ORIGIN      
        1 tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac atttctagct
       61 tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc cgagagacgt
      121 aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac caactagatg
      181 tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct gctttttttt
      241 gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt ttgattgctg
      301 acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag gggtatgaag
      361 cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc cctgatattg
      421 aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta gtgaaagagc
      481 tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact gatatggata
      541 agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt aatccgttag
      601 aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt gccccagatc
      661 agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat gaagttaaaa
      721 caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc tacttgcttg
      781 ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt tggaaacaag
      841 aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga gataagattg
      901 aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc tataaaattg
      961 acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac ttcaaaagaa
     1021 atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct attaaatgtt
     1081 tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac gaatgcgatt
     1141 tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg gaacggccgg
     1201 aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg gcgcttgatt
     1261 cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta catcgcaaac
     1321 ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg agtggtaaca
     1381 agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg taaaatcgaa
     1441 aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc actcacgtcc
     1501 atcattggat acctaggact gatagaagat ggccagtatc actcagaaga agatctttta
     1561 aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt ggacgactta
     1621 tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc ctttgacatg
     1681 atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa aaaaaatatc
     1741 caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac cgaaaaactt
     1801 gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg agcaacgaag
     1861 atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa aaataacgga
     1921 gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg tgtagaagaa
     1981 tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca aagcattgtg
     2041 gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc tttcatcatg
     2101 caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt tattgacgaa
     2161 agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac acagaaccca
     2221 gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa ggataatttg
     2281 gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca acagccgtta
     2341 tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt cagactgatt
     2401 cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga ggaaatcata
     2461 tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa ttgatgactc
     2521 ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat cgtcgtaagc
     2581 tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct gagatcgccc
     2641 aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa gaagtcggtg
     2701 gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg aaacaaattg
     2761 caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat gaagaggaca
     2821 atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca aaaccacact
     2881 gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa gtagccggta
     2941 gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc tacaacttca
     3001 tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct tatatcgtca
     3061 acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat gttttccaat
     3121 tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact aactattata
     3181 atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta agccctgctt
     3241 ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt cttcaccaat
     3301 tcaataaagt agaaatggta aaattttcgg atgtagagca ttcttacgaa gaattggaaa
     3361 aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat cgtgtgatgg
     3421 ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta gaagtatgga
     3481 tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa gatttccaag
     3541 cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat actcatactc
     3601 tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa aactaccaaa
     3661 atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg aatctgaaag
     3721 ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga aaaattttgt
     3781 gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac tggcttttga
     3841 acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag agaaaaaaat
     3901 ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt cagctgattg
     3961 aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac aaggacaaat
     4021 tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag gtggcaaggg
     4081 aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
     4141 aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg aaggagttgc
     4201 ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
     4261 cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct tttgtgattt
     4321 gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata aattgcgctt
     4381 catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa cgcaaaccgc
     4441 cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga ccttcgattc
     4501 cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg tcttttgaac
     4561 ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga cgtccttgga
     4621 agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct agcatcactg
     4681 catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc cctccgtctg
     4741 caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata gctgtgagct
     4801 gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt ccgataccaa
     4861 cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc gtcgcaacgt
     4921 ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt tttcggatca
     4981 cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct gcttctaata
     5041 aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct acaagcaatc
     5101 gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg tctttgatcg
     5161 tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg attttatgtt
     5221 tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc actaagttgt
     5281 cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc atgtcgcggt
     5341 ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc acaccactga
     5401 tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc gttaagaaga
     5461 acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca tccgcttgct
     5521 ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc atagcaatgg
     5581 ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata ttcaacttaa
     5641 tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga ctttctgctg
     5701 gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt tcccagttgg
     5761 acatttttag taccactcct ctaatttagt ttatctatta aaataattga ttttttcatt
     5821 taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa tataaagcgt
     5881 ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa gtaataagaa
     5941 cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta ccgcagatgc
     6001 aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt tcgagtagta
     6061 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt aataccgaga
     6121 aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt ttgtcacagt
     6181 cccttac
//
LOCUS       DAXWLF010000149         5963 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXWLF010000149 DAXWLF010000000
VERSION     DAXWLF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.152"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            20..718
                     /locus_tag="ACGE4U_002273"
     CDS             20..718
                     /locus_tag="ACGE4U_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFF8394391.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(886..3525)
                     /locus_tag="ACGE4U_002274"
     CDS             complement(886..3525)
                     /locus_tag="ACGE4U_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8394392.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(4144..5175)
                     /gene="queA"
                     /locus_tag="ACGE4U_002275"
     CDS             complement(4144..5175)
                     /gene="queA"
                     /locus_tag="ACGE4U_002275"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFF8394393.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            5368..>5963
                     /locus_tag="ACGE4U_002276"
     CDS             5368..>5963
                     /locus_tag="ACGE4U_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8394394.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         1704 a         1298 c         1134 g         1827 t
ORIGIN      
        1 agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag caaaagagag
       61 tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat tgattggtat
      121 tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca tatatgatgg
      181 atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag gcggtggcgg
      241 aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga cttatcttga
      301 tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc gtggattcaa
      361 aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct ttacaacatt
      421 atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt ctcaaagcta
      481 ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac tggatacgat
      541 cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt tagatctttc
      601 atttatcggc tggcatatct tagcattagc aactgctggt attggttatc tatggctcag
      661 cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag atatttaaaa
      721 gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga gaggcagtaa
      781 aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc acacctcctt
      841 tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt cctcttcttc
      901 tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta cgatagataa
      961 aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata gttcttgcgt
     1021 aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca tttctaccaa
     1081 agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta cattgaacac
     1141 atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga catgctgact
     1201 tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac caaacaaggt
     1261 tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc gagcaaagat
     1321 cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg gctctcttcc
     1381 taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt gcacagctgt
     1441 aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct ccgaaatatt
     1501 acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac gtccttgact
     1561 gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg cggcactttg
     1621 cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt tcaacgctgg
     1681 tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt gccaagattt
     1741 gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag ctttgacacg
     1801 cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg cttctgacgg
     1861 atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat gatctccagt
     1921 gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt tactttctgg
     1981 acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt ccagtgcttc
     2041 agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa tcactctcag
     2101 tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc ctgaactaaa
     2161 gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt cgcccatttg
     2221 gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta ctcgtggata
     2281 tttttcttct aattctgcct ttgtatgata attctcttca acagccctta cgatggctac
     2341 ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg tcacatctgt
     2401 acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat cttcgatatt
     2461 cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt gtgtcagcgt
     2521 tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg ctggcaactg
     2581 tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg tcaacgtaac
     2641 aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag tcatgaacat
     2701 ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg cagccgctaa
     2761 agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag gtgttttttg
     2821 catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca tgccaatggc
     2881 agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata ccatattaag
     2941 ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt caactgcttc
     3001 actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga tcaaacgggc
     3061 atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta ccagttgctc
     3121 tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt tattcatatc
     3181 ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca ataccgagtt
     3241 gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt ctgtagcaaa
     3301 tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa aatcggacaa
     3361 gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt tattcaatcc
     3421 ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg gatctgcttc
     3481 tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat tcctccttta
     3541 aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt atttacttca
     3601 aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc ttatatttgc
     3661 aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca tcaaatagac
     3721 agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta caggtttcac
     3781 ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat gtttggatca
     3841 cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt aaacgtctga
     3901 aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa caaaattttt
     3961 gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt tacaatcatt
     4021 tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga gctcggcttt
     4081 ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg gcttttcata
     4141 accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct cttcaatcgc
     4201 atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta acatgactaa
     4261 agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga attcatagcc
     4321 aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat taaatttcgt
     4381 tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt tcccgccatt
     4441 tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga attcactatg
     4501 catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta accctacatg
     4561 tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta attctttcgt
     4621 aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat agacagtttg
     4681 atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca tttctcccaa
     4741 tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc gtccgccgtg
     4801 ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga ttcttgtccc
     4861 aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt gtgtattcgt
     4921 tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata aacgagcagg
     4981 cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt caatgatttc
     5041 atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta aacgagaatg
     5101 atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa aatcaaaatc
     5161 ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt ctatcatgtt
     5221 tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt cctaaataaa
     5281 aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag atagttcttc
     5341 attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc aaaaatttat
     5401 aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg tgagttcatt
     5461 tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat caaccgaatg
     5521 actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga aattgatcca
     5581 gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat gcctcacatg
     5641 acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca agaagaacgg
     5701 aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat gttagatcgc
     5761 tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg tgcccttgca
     5821 gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc aatcactcgt
     5881 gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac gatcgtattt
     5941 gtaacccatg acatggatga agc
//
LOCUS       DAXWLF010000150         5881 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXWLF010000150 DAXWLF010000000
VERSION     DAXWLF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.153"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            225..1169
                     /gene="mvk"
                     /locus_tag="ACGE4U_002277"
     CDS             225..1169
                     /gene="mvk"
                     /locus_tag="ACGE4U_002277"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFF8394395.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1162..2139
                     /gene="mvaD"
                     /locus_tag="ACGE4U_002278"
     CDS             1162..2139
                     /gene="mvaD"
                     /locus_tag="ACGE4U_002278"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFF8394396.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2152..3237
                     /locus_tag="ACGE4U_002279"
     CDS             2152..3237
                     /locus_tag="ACGE4U_002279"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFF8394397.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3234..4298
                     /gene="fni"
                     /locus_tag="ACGE4U_002280"
     CDS             3234..4298
                     /gene="fni"
                     /locus_tag="ACGE4U_002280"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFF8394398.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4547..5350)
                     /locus_tag="ACGE4U_002281"
     CDS             complement(4547..5350)
                     /locus_tag="ACGE4U_002281"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFF8394399.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5370..5693)
                     /locus_tag="ACGE4U_002282"
                     /pseudo
     CDS             complement(5370..5693)
                     /locus_tag="ACGE4U_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            complement(5726..5863)
                     /locus_tag="ACGE4U_002283"
                     /pseudo
     CDS             complement(5726..5863)
                     /locus_tag="ACGE4U_002283"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         1780 a         1248 c         1212 g         1641 t
ORIGIN      
        1 tttctagtca aagaagaaaa aaagtcaaaa aattcttaag aaaaaagtgt tttttcttta
       61 gaaaacgtat tcttagtttt acttctgaga tggttttagt aaaataaagg aggaatgttt
      121 tttgagaccc ttagtggaca aaaaaattcg tgaagcagcg caactcgttt tgggcgcttt
      181 attttttgct taaaaatcac ttttacgtga gaaggagtta gattatggca aactatggcc
      241 aaggagaatc aagcggaaag atcatattga tgggcgagca cgcggttgtt tatggagaac
      301 cagcgattgc ctttcctttc tatgcaacaa aagtcaccgc attccttgaa gagctggatg
      361 caatggacga tcaactggtt tcttcctact attcaggaaa tttagccgaa gctcctcatg
      421 cattaaaaaa tatcaaaaaa ttattcattc acttaaaaaa acagcatgac atccaaaaaa
      481 acttgcaact gaccattgaa agcacgattc ctgctgaacg tggaatggga tcaagcgctg
      541 cagtcgccac agcagtcact cgtgcttttt atgattactt agcatttcct ttgtctcgtg
      601 aaatactatt agaaaatgtc cagctttcgg aaaaaatcgc ccacggtaat cctagtggaa
      661 tcgatgcagc cgctactagc agcttgcagc cgatttattt tacaaaaggg catccttttg
      721 actacttttc tttgaacatc gatgcttttt tgattgtcgc tgatacagga atcaaaggac
      781 aaacaagaga agccgtcaaa gatgtcgctc acctctttga aacccagcct catgaaactg
      841 gacaaatgat ccaaaaatta ggatacttga cgaagcaagc aaaacaagca atcattgaga
      901 attcaccaga aacgttggca cagacaatgg atgaatcaca gtcacttctg gaaaagctga
      961 caatcagcaa tgattttctt aatctattga tccaaacagc aaaagatacc ggggcattgg
     1021 gtgccaaatt aactggcggt ggacgcggtg gctgtatgat tgcattagca caaacaaaaa
     1081 caaaagccca agaaatcagt caagcacttg aagatgcggg tgctgctgaa acttggatac
     1141 aaggattagg agtacatacc tatgtttaaa ggcaaagcac gcgcatatac caacattgct
     1201 ctaatcaaat attggggtaa gaaaaatgaa gaactcatcc ttccaatgaa caacagcctt
     1261 tcgttaacat tggatgcatt ttatacagag acagaagtca tcttttctga tagctatatg
     1321 gtagatgaat tttatctaga tggcacctta caagacgaaa aagcaacaaa aaaagtcagt
     1381 cagtttcttg acctttttcg taaagaagcc ggcctctcac taaaagcttc agtaatcagt
     1441 cagaatttcg ttcctaccgc agctggatta gcctcctctg ccagcgggct agctgcttta
     1501 gcaggagctt gcaatactgc tcttaagctt ggattagacg atctctctct ttcaagattt
     1561 gctcgacgcg ggtctggttc agcttgccga agtattttcg gtggtttcgt tgaatgggaa
     1621 aaaggccatg acgacttaag ttcttacgct aagccagtcc cttccgattc tttcgaagac
     1681 gatttagcaa tggttttcgt tttgatcaac gaccagaaaa aagaagtgtc cagcagaaat
     1741 gggatgcgtc gaacagtcga aacatccagt ttttatcaag gctggttaga ttccgttgaa
     1801 ggggatctat atcaattgaa acaagcaatc aaaacaaaag atttccaact tctcggagaa
     1861 acgatggaaa gaaacggact aaaaatgcat ggaacgactt tagctgccca gcctccattc
     1921 acttactggt ctcctgacag cttaaaagcg atggatgcgg tgcggcaatt gagaaaaaaa
     1981 ggcattccat gctactttac gatggatgct ggccctaatg tcaaagtttt agtggaaaac
     2041 agccatttat ctgaggtaca agaaacattt accaaattat ttagcaaaga acaagtaatt
     2101 actgcccatg ctggtccagg aattgcgatt attgaataaa gggagatttt tatgattgaa
     2161 gtatctgcac caggcaaact ctatattgcc ggagaatatg cagttgttga aacaggacat
     2221 ccagcagtta tcgctgcagt cgatcaattc gtaacagtca ctgtagaatc cgcacgaaaa
     2281 gtcggaagta tccaatctgc acaatatagt gggatgcctg tacgttggac aagacgcaac
     2341 ggagaattag ttttagatat tcgggaaaat ccttttcatt atatccttgc tgctattcgt
     2401 ttgactgaaa agtatgcaca agaaaaaaac attcttttat ccttttatga tctgaaagta
     2461 acaagtgagt tagacagttc aaatggccgg aaatatggat tgggttctag cggtgccgtg
     2521 accgttgcaa cggtcaaagc cttgaatgtt ttttacgcgt tgaatttatc tcaattggag
     2581 attttcaaga ttgcagcact agccaattta gcagttcaag ataatggttc ctgcggcgac
     2641 atcgctgcta gctgttatgg tggctggatc gctttctcaa ccttcgacca tccttggctc
     2701 caagaacaag aaactcagca ttctatcagt gagttacttg ccctggattg gccaggtcta
     2761 tccattgagc cattgattgc tcctgaagat ttacgtttat tgattggttg gacgggtagc
     2821 cctgcctcta cttctgattt ggtcgatcaa gttcaccgtt cgagagaaga caaaatggtg
     2881 gcttatcagc ttttcttaaa aaacagtaca gaatgtgtca acgaaatgat caaagggttt
     2941 aaagaaaata atgtaacgtt gatccaacag atgattcgaa aaaaccgaca attactgcat
     3001 gatttatctg caatcactgg ggtcgtcatc gaaacgcctg ctttgaacaa attgtgtaat
     3061 ttagctgaac agtatgaagg agccgcaaaa tcttctggtg caggtggtgg cgattgcgga
     3121 atcgtaattg ttgaccagaa atctggcatc cttcctttaa tgagtgcatg ggaaaaagca
     3181 gaaatcactc cactgccgtt acatgtctat agcgatcaaa gaaaggaaaa ccgatgaatc
     3241 gaaaagacga acatgtttca ctagctaaag cctttcatga taaacaaaaa aacgaatttg
     3301 acttcgtacg gatcatccat aaccctttgc cgcaaatagc agtttctgat gtcgatctaa
     3361 gtacacaggc agttgggttt acactaagca gcccatttta tatcaatgcg atgactggcg
     3421 gaagcgaaaa aacaaaaaaa atcaaccaag atcttgcaat cgtcgcaaga gaagcagatt
     3481 tgatgattgc cactggttca gtcagcgctg ccttgaaaga cccttcttta gccgatactt
     3541 atacgattat gcgacaagaa tatcctcacg ggaaaatcat tgccaacatc ggtgcaggga
     3601 cttctgtcga aagagcccaa gaagctatcc gattgtttca cgcagatgcc ttacagattc
     3661 atttgaatgc acctcaagag ttggttatgc ctgagggaga ccgtgatttt accaattgga
     3721 aagtacttat tcaagagact caaacagcca ttgacgtccc cctcatcgta aaagaagtcg
     3781 gttttggcat gacaagagaa acgctcaatg atctagctgc tttaggagtt catacagttg
     3841 atatcagcgg tcgaagcgga acaagcttca cccagatcga aaacgcccgt cgctccaagc
     3901 gggaactgag ttatttagct gactgggggc aatcgacagt ctcttcattg cttgaagcaa
     3961 atgaagcaga cacttctatg gaaatcctag cctccggagg tatccgcaat gcctatgaca
     4021 tatttaaagc cctttgtctc ggcgcaaatg cagtgggtac ttcaggaacc gtactgacgc
     4081 atctgatgaa tcacggcgta gaagaaacta ttatactgat gaagcaatgg caagaagagc
     4141 ttcgtctgct ttatactatg gtcggcgcaa cgaataccgc ggccctacac caacaatcac
     4201 tgatcttttc cggaccagtg aaggattggt gcgaagcaag agggatcgac ttagtgaagt
     4261 atggaagacg cacagaaaag agcgtgggac aaaagtaaag aacactttta tcccacgctc
     4321 taaacacgta taaacggcgg aagcaaaagc aactccttcg gaaataagct gaaattcaca
     4381 aaaatttgaa aagcaatttt cgtgaattcc ttcttatttc ttggagttaa acgcttctgt
     4441 cccagcctct tttttcgaat aaatgatgtt taatcagctg tccttcggta ttcgttcaag
     4501 ccgctgattt cacaacctct ccactaaacg gtgtactaaa gctcttttaa ggttgttttc
     4561 cgatataggc aagtatgcct ccgtccacat acaagacatg gccgttaacg aaatcagatg
     4621 cgctagaagc caagaagaca gatggtccag ccaaatcgat tggctcgccc cagcgtgctg
     4681 ctggcgtacg tccaacgata aattgatcga atggatgacg ttcaccgttt tcttgtgttt
     4741 cgcgtaatgg agcagtttgc ggtgtagcaa tataacctgg tccaattcca ttacattgga
     4801 tattgtattg accgtattca gaagcgatgt ttttcgtcag catttttaat ccgccttttg
     4861 ccgcagcata agcactgacc gtttcgcgtc ctaattcact catcatggag cagatattga
     4921 tgattttacc gccgcctttt tcgatcatat cccgaataac tgcttttgaa acgatgaatg
     4981 gggcatttaa atccacgtcg attacttggc ggaagtctgc cgcatccatt tcgatcatcg
     5041 gtgtccgttt gatgatccct gcattgttga ctagaatatc aactgaacct acatcttctt
     5101 tgatctgttg aaccatcgct tgaaccgctt cttcatcggt cacatcgcag acataacctt
     5161 ttgcatcgat gcctgcttct ttataatgcg ctataccttc atcaactgat tcttgattca
     5221 agttattaaa tacgattttt gctcctgctg tagctaaaga gcgtgcaatc tcaaatccaa
     5281 taccatagac agcaccggtg actaatgcta cttttccatc taaacgaaac atatccatac
     5341 taaattccat cattttctct cccttatgct tattttaatt gatccatttg caccatatcc
     5401 atgtctgtat aggtgatatt ttctccgcac attgcccaga tgaatgagta attgcttgta
     5461 cctacacctg aatggatcga ccagcttggc gaaataatgg cttgttcatt gtctacgact
     5521 aaatgttttg tttcatctgg tttgcccatc atatggaaca cacgtgtatc ttcattggca
     5581 taatcaaaat agacataagc ttccatacga cgttcatgtg tatgacaagg catcgtattc
     5641 caagcacttc ctggttctaa aatcgtataa cccatttata aatgacagct atcgctgaag
     5701 cttctactgc tgtaaatatc cccgtcaaga taccaccgat aatgatgaca atcaatagaa
     5761 ggctaggaat cgcctctttt attatttttc cagcatgagc catctgtcct ttttcgacaa
     5821 cagggtattt attcttttta gcaacgatga atgctactag catgattcct agcgcccaca
     5881 a
//
LOCUS       DAXWLF010000151         5795 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXWLF010000151 DAXWLF010000000
VERSION     DAXWLF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.154"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            30..3308
                     /locus_tag="ACGE4U_002284"
     CDS             30..3308
                     /locus_tag="ACGE4U_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFF8394400.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3849..5756)
                     /locus_tag="ACGE4U_002285"
     CDS             complement(3849..5756)
                     /locus_tag="ACGE4U_002285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFF8394401.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
BASE COUNT         1817 a         1112 c         1208 g         1658 t
ORIGIN      
        1 ctttctaatt tgttacggga ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc
       61 taatgatgaa tctccttatg ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg
      121 ctaacgaaca acagtctgtg aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa
      181 caatggagga aataccaaga actagtgaag atatggatac ggaagaagga aatgatccta
      241 tgatgaaaga atcagaaaat gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt
      301 ggaaaggggt tgttttcgga gaaagcacaa gtgctagtag gtatgccatt gaaccattag
      361 aagacggcgt gcggctctct tccacgaata acggcggaaa attccagtca agcggatcag
      421 atggaatgac ctattattat caagcaatcc ccaaagacat caactttagg atgcgggcga
      481 cgatcgaaat agaatcttgg acctatacga atgcccaaga aggatttgct ttaatggtaa
      541 gagacgcagt accaaaaaat catttgtttg ggcaggcatt ttattcaaat tcatttgcca
      601 tactaggcag tcggatcgaa tatgtctggg actcggatag acaagaagtg gtcaacacga
      661 gcggcagcaa atatacgatg cgacttggat tggggacacg ggcaatcaca ggaatatcat
      721 ctgaagatcc gcaagctgcg ccagctcctg ggagtgtctc agtggaaaca acacctttag
      781 agacttcagc agggttcttg gaaaaagaaa caggttctta taatatattc ggaaacggtc
      841 atgggaatct gtttccggcc acgaactcga ttactaaact ggacgtagag atgaaaaaaa
      901 cgaatactgg tttagaggca tattattacg atcctgaaac aggagaagaa atggcttctg
      961 acatcatgta tgactgggaa aaattatacg cttcagatga atcggttatt tatgcaggat
     1021 ttgctgcagc aaggaatatg acgatcaagg tagaaggaat cgaagttgct tactcagatc
     1081 ctgcgacaga tcctcctggg caagaaagac ctactcgctt gattgatccg atttacactg
     1141 tgacctctag taatcattca ggaaaccaag atcatactat gtcctttaga gcaaatgctg
     1201 acggattatt gacaattaaa aataaagcaa caggagaggt gcttaagaac gcaaaaaact
     1261 tggctatcac tgcgaatcgg gaatttgatt atcagacaaa actgatggaa ggaacgaacg
     1321 aatttacttt cagttttacg ccagataaaa actatccgcc agaagaatac gtagagatga
     1381 ctagttatga aacaaaagag attcttcata cagtggacta ccgtaaacct aaatattcga
     1441 cagtttatgt aacaccagaa gggtcagatg caggaaacgg aagcagacaa aatccattat
     1501 ctctgacaca agcattgaga tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa
     1561 cttattcctt cgaaagagga ttgacgattc caaaaggcgt taacgggaca agagaaaacc
     1621 cgattcagtt gattgcagag aaaaatccag aaaacaagcg tcctgtactt gattttaaag
     1681 gaactggaaa tgggatgaca ctgttcagtc actactgggg tttacgaggg tttgacatta
     1741 caaatagtgc agctatgcaa aaaggattac agatttctgg aaatcataat ttgatcgaag
     1801 agattcatgc ttatcggaat ggaaatacag gaatccaaat cagcggtagc tcaaatgatc
     1861 ctttcgaagc atggcccgcg cataacttag tcaagaactg tacatctttt gaaaatgccg
     1921 atccaggatt tgaagatgcc gatggatttg cggccaaact gacaatagga gaaggaaatg
     1981 tatttgacgg ttgtatcgcg tatcataatg cagatgacgg ttgggacctt tttgctaaaa
     2041 atgaaacagg ttcgatcgga aaagtaatca tcaaaaactc agttgcttat cgaaacggct
     2101 gggtaccagg aattgaggga gaaggtaacg gtaatggatt caaaatgggc ggttcttctt
     2161 taacgggccc tcatgaattg atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg
     2221 attccaatag cggaacagat attatcgtca atagcagtac aagttttaat aacggttcat
     2281 ataatgtggc tctttatacc tcttcagcag gaaacacaga ctaccatgct tctggtatct
     2341 tatctttccg aacagagaat cttgagatga gagagcagat ccgcccattg aatcaaaacg
     2401 aagatcaaat ctaccactcg ttgaactact attggaatga acgagagaaa caatcagaaa
     2461 atactcttgg acataccata tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag
     2521 aaggtcagca gcctgttacg cgctatgaaa atggaagtat ccatgttcat ggactgatgc
     2581 agattaaacc ggggaatcgt caaacagaaa aggaaagagt ggatggtctt ccattatctg
     2641 taggtgctat ttttgacgga gaaacctcac cgtcttcgga atatccagag tatgcaatcg
     2701 tacaagaacc aaatcagaca aaaccaaatg aacaaaataa tccgattatt atacaagaag
     2761 caaatggtgt ggcggagaat gcagaaggaa atatcattga actgaagcct tttacagttt
     2821 ttgtgtcaac tttcacacca aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc
     2881 cagaaaagat ccaaatcgtt gaaacacagt caagtgctca attttctaat cataccgcta
     2941 ttttttcagc accttcaaaa ggagaatata cgctagaagt cacttatcgt tcggaaatat
     3001 ttgatgggga aaaatggtct gctcatacag aatctgttac ccagcaaaaa gcgatcactg
     3061 tgcgagaaac tgccgatgtg cccccgggaa atgacaaacc agaaagagaa aatccaggaa
     3121 atgaatcacc aaatggaaaa ccaaatggaa aaccttctca tccggatagg acagcaaaag
     3181 aaagtgaaaa aaatggcact caaggactcc caaaaacggg agaatttaat aacccgttgc
     3241 tagcattagc tggaggaatt ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa
     3301 aaaaataagt ggaatttctc cttttatgat gtaaaacaat tgactgagga aagtaaaaat
     3361 agaaggaaat gagaccaata gatgtctcac ttccttctat ctttttatag atgaggttct
     3421 ccttgctgct tttgattggc tgttcttatt acttgatgca ggacagcttt ttcacaatct
     3481 ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa cggcaaaata
     3541 tgaggaacta tttttccgtt tttttctcta atactcaggt caggacgctg atttcacaac
     3601 ctctcgataa acggtgttca aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa
     3661 tgtggaaagc cattttcaca aaatttttct tactacttga aactaagcag ctttttcaca
     3721 acctctaata catggtattc cagaagtaac ttcttcgaaa aataagccga aatgtcacaa
     3781 aaatttgaga agcgattttc ccaaattcct tcttatttct tgaagctgac cacttctggc
     3841 acaacctctt accagattcg cactcttttt tctggcgcta gatacatagc gtcttgttcc
     3901 gtgataccaa atgtttcata gaattcgtca aggttttttg gttgcacgtt tgctcggagt
     3961 ttagcagggg catggacatc gatttgcagt aaaagttgtt ggtattcttt ttttgctttt
     4021 gtacgccaaa tcgttgccca attgacaaag aattcctcga tagaaagatc tgattccgtt
     4081 tttgctgctt ccaatgcaca gcttagaccg cctgcatcag caatattttc cgaaacggtt
     4141 aattttccgt ttacttttcc gtcagcaaaa ggcagaccat cgaattcttc gatcattgct
     4201 tgtgctttgt tttgaaattg tgtaagatct tcttctgtcc accagttgtt caagttgcca
     4261 tattcgtcaa ataaagcgcc gttattatca aatgcatggg aaatctcatg agcgataaca
     4321 gctccgattc caccataatt agcagagctt gattgttcta gactgtaaaa tggtgcttga
     4381 aggatcgctg ccggaaagac gatcacattc cgaaatggat gataataagc attgaccgtg
     4441 tcagcactca tttcccattc tgaacgatcc actggtttgt tccatttgct gaaacgatct
     4501 tttaatgtga gtttgctgaa aacaagagta tttgaaagca aagtaccacc ctgatctgca
     4561 ggtactgttt tgaactggtc gaataatgct gggatcttat cggggtagcc tacttggata
     4621 cctaagttat ttaattttat gacggctttt tcttttgttg catccccaag ccagtcattt
     4681 tcttctagac gttttttata aacggcgatc atttttttca ccatttgttc tacgtctttt
     4741 ttagcagctt cgccaaaata tttttttccg tagtaatcgc caactacttg atcgaattgt
     4801 ccagaggcaa ggtaatacgc tgcttttttc tgaggcattg cttcgtctgt tccagacaat
     4861 acgcgagaat agattccgct gacttggcgg aattcttctg atagataacc actaagagaa
     4921 cgtactgttt taatgatcat ccagcttttg aataacggaa aatgagcttc tgtcaggatg
     4981 tcattcaaat gatcgaagta cactggatca gttacgatca ctttatctgg agttgtccca
     5041 atcaaacccg tgacaagttt tttcaagtcc agtttatctg tgtatttaat aaatgtatca
     5101 aaagattgcg gattatacat tttgctgtaa tctgcgtttt cttctgcact ttttacatga
     5161 ggagcaatca atttatcaaa ttgcactgct tgttccgtga ttgcttctgc ctgacttttt
     5221 tctttgccgg caagctctaa taactgaaca gccatatcaa agaaaacgcc tattagttgt
     5281 gcaccgttgg gatggtctga tgcataatac gtcttatctg gaaggaataa agaaggcgga
     5341 taagcaaaga aggcgttcgt ttgcgcattc ttcatatctg catctacatc cagattaaat
     5401 ggcaatggta atgtatccaa cagccattct ggaaattgct tattcaattc gtcgaaagac
     5461 tgcaatgatt caactttttc gattataggt tgaagcggtt gagcatgcaa ctcatttctt
     5521 cggtcataat cgtttgccag ttgataatat gcaaggaaat gtttcatttt ttctgaagac
     5581 actttttctg gatgggcaag cagatcgtct gtttctccca tcaaaagttg gtcgatcccg
     5641 tcaactaaat cttggaaacc gcctgtagct ggtttatctg cagggatttt tgctgttttt
     5701 tgccattctt cattgactgc ttcatagaaa tcttgtttca atcgttcttt gttcattcat
     5761 cgatcgctcc ttttttgttt cgtgtgccca ttatc
//
LOCUS       DAXWLF010000152         5783 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXWLF010000152 DAXWLF010000000
VERSION     DAXWLF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.155"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(9..467)
                     /locus_tag="ACGE4U_002286"
     CDS             complement(9..467)
                     /locus_tag="ACGE4U_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFF8394402.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(480..1811)
                     /locus_tag="ACGE4U_002287"
     CDS             complement(480..1811)
                     /locus_tag="ACGE4U_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFF8394403.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1822..3264)
                     /locus_tag="ACGE4U_002288"
     CDS             complement(1822..3264)
                     /locus_tag="ACGE4U_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8394404.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3251..4015)
                     /locus_tag="ACGE4U_002289"
     CDS             complement(3251..4015)
                     /locus_tag="ACGE4U_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFF8394405.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4119..4994)
                     /locus_tag="ACGE4U_002290"
     CDS             complement(4119..4994)
                     /locus_tag="ACGE4U_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8394406.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5249..5333)
                     /locus_tag="ACGE4U_002291"
     tRNA            complement(5249..5333)
                     /locus_tag="ACGE4U_002291"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5297..5299),aa:Leu,seq:caa)
     gene            complement(5351..5422)
                     /locus_tag="ACGE4U_002292"
     tRNA            complement(5351..5422)
                     /locus_tag="ACGE4U_002292"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5388..5390),aa:Gln,seq:ttg)
     gene            complement(5432..5504)
                     /locus_tag="ACGE4U_002293"
     tRNA            complement(5432..5504)
                     /locus_tag="ACGE4U_002293"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5469..5471),aa:His,seq:gtg)
     gene            complement(5566..5650)
                     /locus_tag="ACGE4U_002294"
     tRNA            complement(5566..5650)
                     /locus_tag="ACGE4U_002294"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5614..5616),aa:Leu,seq:caa)
     gene            complement(5671..5741)
                     /locus_tag="ACGE4U_002295"
     tRNA            complement(5671..5741)
                     /locus_tag="ACGE4U_002295"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5707..5709),aa:Cys,seq:gca)
BASE COUNT         1753 a         1237 c          891 g         1902 t
ORIGIN      
        1 atcgtacact aaaattcaaa acaactatct ttaatcttta cttcttggtt ttgttttaat
       61 aaatttcctt caaaaatgcc tggagaaact acaatgatca ccgtatcttt ttgtgcttgc
      121 tccaacttct tcaaatctat aatagctaac agtgtccctt cttttacctc ctggttactt
      181 tcaacccgta tctgaaacgg acttccttcg agttcaactg tatctaaacc catatggatc
      241 agtatttttc tgccagattc actttcgatc gttatggcat gtcgggtagg aaatatatta
      301 tcgattattc ctttaactgg cgcatggata tatccatcat gattcgttat agcgtatcca
      361 actcctatca tttttgaaga aaagacctcg tcttttactg ctgttaatgg aataatttga
      421 ccactagcta cagcttttag aatgtttctt gttttaaaga aatgcacttt ttatcacctc
      481 tactttaatg ctggccaata ttttttgttt actttgatca tttcatctag tatttttttt
      541 gcgactctgg catttggtac ggtttgattc aatgtaaatg cttgtaaagc tttttgataa
      601 gaaccttcaa agtaagcatc tactagtaac ttctctactg caacttgtgc ctccatcaat
      661 cctttatgaa aatcactgat tggttttctt agtgtaattg gctccacacc agttgcacca
      721 acatatgccg gtatttcaac aactgcatct gaccgtagat tcggaatcgc cccttgatta
      781 ggaacgatta gcataaagcg acgacgatca tcattcaaca aggaaatagc tatatcgacg
      841 atatattgtc catgctcccc aaaattaaag ttcaatactt cttccacaat accgcggcgt
      901 attttatcag ccatttcttt ggtatttttc tctcgtccat ccatgaccat attagcacgt
      961 gtatactctt tgtcagtatg ctcaacgacc atatcaggat ataagtaata ctctaagtaa
     1021 ttattcggga tattacttgg aaaatttttc gtgatgactg acatcatttg aaaggttttt
     1081 tgccaagttt tatctcctat attaaaatcg gcgacttgca tttcttttgt tattagtttt
     1141 tcaataatct ctggcataat ttctcgttgc aattctttat cataaatcga tgtataccag
     1201 ccaaagtggt ttaaaccata atacgtaacg atccaattct tacgatcata accgtaattc
     1261 actgcaatcg tttcttcaat tgaaatagtc atatcacagg cattaatcat ttttacattc
     1321 ggatatttac gacgaacagc ttcagaaacg atcgactctg gatttgtata attcaaaatc
     1381 catgctttgg gggcatagtc ttgtatatga tcgacaattt ccaataaacc tttgattgag
     1441 cgcatcccat aagcaaatcc tccgagtcca cacgtttctt gtcctactaa tccatgttta
     1501 agtggaatct tttcgtcctt ttctcgcatc tccaatcctc caacacgaat ttgtgagaaa
     1561 acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat gcgaatattt
     1621 gaaaaacctt ttcttttcag cataaattta acaatcaagt acatatccat atttctttct
     1681 tcatcaatat catataaacg tatttcagat aatggcaacc gtttttcatt gttcaaaatg
     1741 gcttgaacga ttcctggtgt atacgtactc ccgcctccag ctatagtgat aatctgtttt
     1801 ttcatcatca tcttttcctt tctattcttc taataattta ttgaattctg ccataacata
     1861 agtcacttct aagccgatga taatttgata atttttttca gttggtttaa tgatcccgct
     1921 tatccctaat tgcttgattc gctgttcctc gaccatggtg ggatccacta cctctacccg
     1981 taggcgtgta ttacaactat agacatccag gatattggct tttcctccaa acgcttgtag
     2041 catttttgtt gccatatatt cataattttt acttgctaac cctaactctg tttcttctaa
     2101 ggttacttca ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa tgtttctctt
     2161 attgatcaaa taataaaaag taacgtaata caaggcaaaa aagaaaattc cgattggaag
     2221 aatcaaaata gcattggtag cgattcgaaa attgatgaga taatcaacaa tacatgctcc
     2281 gaatgtaaaa ccaattcgta tattgaataa gtaacagaca atccctgcta aaccggcaaa
     2341 aagtgcatgg atcacgtaca acataggaga aagaaacatg aaactaaatt caacaggctc
     2401 agtgatattt gcaataaatg aagttcctgc accgcttccc atgattcctt tgaccatctc
     2461 tttattttct ttataagctg ctttatagat agctagacat atagctggta taccaaacat
     2521 cattactaca aaaaaaactg ataaaaacag tccagcctga ggatctccgt tgataaagcg
     2581 tgtcatctct cctctgaaaa cttccttgct aggagatgta tagcttccta aatcgtaata
     2641 aatatacgta ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat tcaaaaacat
     2701 aaatatcccc acacctaatg ctcccatatc aacgagcagt tttgcaaaat tatcaatccc
     2761 aatttggatg agcggccaga taaagcccaa aaaaacagct gcgattgttt gtagaatcat
     2821 caccatagtc aatgggaatt tttcgcccgc aaaataagaa aaaactactg gtaatttttg
     2881 ttctttaaag cgattatacg tccatgccgc tactaaaccg gaaataattc caccaaaaat
     2941 ccccatattc acttctgggt ccataatttc taagcctttt ttcaaggtag caatcgctaa
     3001 aaaaccagta agtgcaggta ttcctttgtc cttatttttg acaaaaccga ttgcaactcc
     3061 tatagcaaat aagatatcca agtttccaaa aacggcattt ccagctgcac caattactgg
     3121 gatgttcagc atatcgtcag ctgataaacg catgatcaat cccgctaatg gcatcgctgc
     3181 aactggaagt agcatcgctc ttccaagctt ctgcacattt gatttaacat tcatttttat
     3241 tcctcctcta ttaaatatcc tcatatgtta ctaactcaat caaaggatca tttttcactt
     3301 tttgagcaaa tgctgaatga attaaaaatt cgacttggtg ggtacgagga tacacaaaac
     3361 ttgaaccatt taacaattcc tgatccaaat acgctgcgtg acacatgact tcgatcgttt
     3421 tatcttctct caatatcatg agtaaacttg ccaaatattc ttccacactc gtattgattt
     3481 gtcgtttttt ttcatcagtt tctcctacga catcgaaaca aggctcaaaa taatcgacat
     3541 gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg caaatcatat ttttttgcta
     3601 atgtaacaac tacctcctga ttttcatcca aagtatgagt atgatgatga ctgtctaaat
     3661 gagttggtat gatccccgca tgataaattt tttgaatctg agcattccat tcctgataca
     3721 gttgatcttt gtcaatgtta aatggatgct gataataaga taaggatctg aactctccgc
     3781 tttcgataag agtatcgaca gttttgagga gtggtttgcc acacgtcaat gttaaatgga
     3841 ctcctacacc tagcctaggc atttcctttc ttagtttcac agcatgctcg aaacccggca
     3901 tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc ataatcccat
     3961 aattcacacc atggctatag ccaaaatcat ccgaattaaa aatcactttt cccatttcat
     4021 tctccttctt ttttctacta aaataggcgc aaccaaataa tcatagtcca tttactatgt
     4081 gtcatttggt tgcgcctata gatagttacg cccttatttt aatagattct tgctagatga
     4141 attgccaaat agatgatttc attttcttga atggcaagtt gaaattgctg ttcgatgata
     4201 tctttaatct ttagtgctgt acaatacgct ttgctatgtt taaatgtttc agtaaaaaaa
     4261 ccaatatttt ctactggcgt ttcttcctga taaatcaagc gtttaaccag gaatttgata
     4321 tgaaccatta agcgatgata agtcagtgat tctttatcat aaacgtttga ataggatttt
     4381 tctagatggg acaaaatcgt ggtcgtcatt tctactattt ttaagctctt taaactatta
     4441 ggctctccta gctcagcatt aatgatatgc atagtgatga atcctacttc atttgaccct
     4501 aactcggttt gatatcgatg attgattcgc tcgaccactt ctactgctat atcatattca
     4561 tttggataaa ggaatctcat ttctggcaac ataattgctg tgacatctct atcttctctt
     4621 tttcgttgaa tagcaaaata aatatgatct gtcaatgtaa tatagatatt tgaatctaat
     4681 ggagaggaga tcctagctgt tatcagttca atcacttctt ccgcaaaatc cataaattcg
     4741 attggaatct gcgagactat ctcactgaat cgtttgatct tttctttaga atctaataca
     4801 aataatttgt cagcatcctc aacatcaaat ggcttactca ctttagcaga aaagccaatc
     4861 cctttcccca tcgcaatttt ttctattcca tcgtcatcga tcaatacagc attatgattc
     4921 agctttttca tcacatacac cgttaattca cctcctctat atgataaaac aaaacaactt
     4981 gcagactcct tcatacatat ttttgtctcc aaacaatttt taaagacaaa aaacgaccta
     5041 agaaagaacc ctttcttagg tcgtgcctaa ttgaatagta gcaccctaca taatttacaa
     5101 aaatagttta acacataata aaagcgttat caatatcttt tttactcgtt ttatgataat
     5161 acttgtatcg aattgatttg gaggtaatta cataaaaaca aaaaaaatat agcaactctc
     5221 tataagaaag ttgctatatt gataattata ccggcggccg gggtcgaacc ggcacgtcct
     5281 caacggacac tggattttga gtccagcgcg tctgccaatt ccgccacgcc ggcaaattta
     5341 aaaataataa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa agtcccttgc
     5401 cttaccgctt ggctataccc caagaataaa gggcgaatga tgggaatcga acccacgaat
     5461 gccggagcca caatccggtg cgttaaccac ttcgccacat tcgccatcat aaaagatgtt
     5521 atttaatttc tcaataaaaa actaagatga agacatctta gtcaataccg gcggccgggg
     5581 tcgaaccggc acgccctcaa cgggcactgg attttgagtc cagcgcgtct gccaattccg
     5641 ccacgccggc aagttgattt attagtacta aggcggtaac cggatttgaa ccggtgatga
     5701 aggttttgca gacctctgcc ttaccacttg gctataccgc cattatacta ttatcaatct
     5761 ataaaataaa gaataaatta tag
//
LOCUS       DAXWLF010000153         5775 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXWLF010000153 DAXWLF010000000
VERSION     DAXWLF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.156"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(235..1017)
                     /locus_tag="ACGE4U_002296"
                     /pseudo
     CDS             complement(235..1017)
                     /locus_tag="ACGE4U_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1082..1918)
                     /locus_tag="ACGE4U_002297"
     CDS             complement(1082..1918)
                     /locus_tag="ACGE4U_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8394407.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1936..3345)
                     /locus_tag="ACGE4U_002298"
     CDS             complement(1936..3345)
                     /locus_tag="ACGE4U_002298"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8394408.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3359..5182)
                     /locus_tag="ACGE4U_002299"
     CDS             complement(3359..5182)
                     /locus_tag="ACGE4U_002299"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8394409.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5462..5689)
                     /locus_tag="ACGE4U_002300"
     CDS             complement(5462..5689)
                     /locus_tag="ACGE4U_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394410.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
BASE COUNT         1770 a         1257 c          944 g         1804 t
ORIGIN      
        1 acaaatttct taaaaatttt ttcactaaaa tcactacctg aaacagtcta aaacacttat
       61 atatcaacgt ttaatgaaac tatctccgtt tcatttcaga tacaaataaa tatagtcggg
      121 ctccaaatcc gaaaaaaaat acccccagta gcttatccta agctactgga gatgtataag
      181 tacggtcata taacaacggg gtagggtaaa taaatgaaaa agatggaaga atcattattc
      241 aataactttt ttaaatactt cgttgtaatc agttacctgt ccgttcttaa ataccacaga
      301 ataatctttt tacccatttt caagtaaccg aagtggaatg tctttaccaa cttgctctgt
      361 cactgcatta ctgatacctg ttagattagc taataaatct gataaagatt gagcatctgt
      421 agaaatctct gtatcagtat ttatcgtgat tcctttatct tcatttacaa gcttccaagt
      481 aggtggtata gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta tcgtcttccc
      541 agtatctact tctgtctcat tactcttaga tgaatcgatt ctttcatcca tcgtctgctc
      601 cgtaactgct tctgtctcat tattctcacc ggaaactctc ttcacaccca aacttatata
      661 ttgtccatct ttagtgtgca tagttaaaat tcccacgctt ttactaataa attcactata
      721 accagttctt tcaatagaga cttcaatgta tttcacacta tcatactcaa aagaaaaata
      781 gacatgtaaa agcattttgt tgtttacatc tacataataa tcctttgact ctacttgaga
      841 ccttcctata cggaatacag ctttactgtc tgatatctct ataccagcat ccttattatt
      901 aatctgccaa tctccaagta gctcttctgg caagctagta gtttcttgtg aacactttac
      961 cacttcactg gtagaggtcg aaacattcac ctccgcactg ttagaagaat tttccaagga
     1021 atcaggagcc ttagcacaac caacaagtct ttatatattg tgcagccgat attttcttca
     1081 ttcagtacac ccttgaaata tgcagtgtta gataaagcag ttcgtcctga tttgctgagg
     1141 cacccatctc ttcttgaacg tactccttga ttttcaaggc cgcccgatac tctttcggaa
     1201 aacgctgctt cactgaagcc agcaaaaaat catccttctc cggcaactct tccttcttaa
     1261 tcattcgggc aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc atcgaatctt
     1321 tatcaacctt tttttcggga aacagatatc ctagcatagc caagatatcc tttagtgctc
     1381 ttaccgagag catggtttct tccatatttg cttgttcact ttgggcattt acaatgtgaa
     1441 acgcaatgtt tccagcctcc tcttctggca actgaacatt catttttttg ttgatcaatt
     1501 ggatagcata gcagccgatc tcaaattctt ttgggtaata cttttgaatt tcaatcagca
     1561 ggcgattttg aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc aagtgatcaa
     1621 ccaaggtaaa aaatagactt ttattcagct taaagtcaaa cttggcgttg gcataatcga
     1681 taattcgctt gacgcaaata attagatcat ccggagcagc ctcaaacagt ttaatgtaat
     1741 ccttttttag agcatcagaa agtgaatcta atttaaatac tttttggatt ttgtcagccg
     1801 gaaccaaatc gcctttcttt agttgaaatc caatcccttt ccccatcaaa attacttctt
     1861 tgccttgctt atctaagcaa agaattaaac tgttgtttaa cgttttaatt actctcatta
     1921 gattcaccgt tctctctaag ctaagccatt tcttgcgatt actttttgat accaaaagaa
     1981 gctttctttt tttattcgtt ttaattcttt aagattctca tcggttcggt caacatatat
     2041 aaaaccataa cgcttattga agccttgatg ggtactaaca acatcaatcg ctgaccaagg
     2101 taaataacca aaaacatcta caccatctgt taaagctgct tgaatagcca gaatatgttt
     2161 ggctagataa tcaattcgat cctggtcatt aattcgtcta tcttcctcaa ctatgtcggg
     2221 tgccccatag ccattttcag taattaaaat aggtaaatca tagcgatcgt acaatttcct
     2281 caatgttaat cgtagtccag tagggtcaac tacccagcca tatggcgttt tatctacata
     2341 aggattttca gctgcccggt agacaccttc ttctcccaac atgatctgtt gatcccccgc
     2401 tctagcggaa acatctgatc cgtcattttt gctggcagcg atggtggcag ttgaataata
     2461 attaatcgca ataaaatcgg gtttaccgga ggataaaatt gcctgatcct ctgcctcaat
     2521 tttaggatat aatccacgat cattcaaata gttttcaaac aaataattgt attttccccg
     2581 aactgctaca tctaagaaag accagcctct caatgtttcc caattatggg ctgcaattgc
     2641 atcttctgga ctggaggtag cttgatacat agccgttaaa ttgattgctg gaccgatttt
     2701 tcctttaaga ttcaactgat gatacaagtg aattactcta gcttgagcta ataaaacatg
     2761 gtgattaatt tgaaagagtt ctttttttga aggcagctct ccacttggtg gcaaaccaat
     2821 cgctccagga tgaaggatca tcgtgttttg ctcattaatt gtcagccaat agttcacctt
     2881 gtcaccatac tcagtaagca atacctgtac atagtccaca aaataatcga tagttgctcg
     2941 atttaaccaa ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt acatggtgac
     3001 aatcggttcg attccctgat tcaggcattc atcaattact tgatgataaa aagaaattcc
     3061 cttttgattg gttttattat tttcaatatc cataattcgc gtccaagcga tggaaaagcg
     3121 aaaagctttt aagcctaatt ctttaaacag ctgtatatct tctttatagt gatggtaaaa
     3181 atccgaggca acttcaaaat tagctacccc ttctgtatgc tgatacttgt caataataga
     3241 cggttgtttc ccatcctcct gataggcacc ctctgcctgg taggcactaa tggaggcacc
     3301 ccataaaaac ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa tcctctcctt
     3361 aactaatttg catcaatggt tcgtgttcct ttaagcttcc gcttgcttca ctaattcctg
     3421 cgtaattttc actgttggtc actaccacaa tcactgtatc atcgtagcca gcgtttctaa
     3481 tggcagcgaa atcggcgttg attaataatt gtcctgctga cacctgatct cctgctttta
     3541 caaatacttc aaagggttcc ccttttaaat tcacggtgtc gatccccaca tggatcaata
     3601 attcaatacc ttctttagaa cgtagaccaa tcgcatgttt agattctaaa atagtaatga
     3661 cttctccctc aacaggtgaa taaatgctgt tttcttctgg atgaatggct acgccctttc
     3721 ccaatagtcc cttagaaaag acagcatcgt tcacttcaga aataggacac acggttcctt
     3781 ttaccggatt ttcaacaatg actgcttcgg ctattgccgt ttgctctgat tcatcagaag
     3841 tatcgtcaaa tccaattaga taagcaatga taaaggttgc aacaaaggcg attacacacg
     3901 taataattgc gtgaacaata ttcattggat tttctccaat aaaggctggt aaggctgcta
     3961 agccagggga aacaaaggca tatctgacta aaccgctcaa gccagcatac aaaccagcaa
     4021 ttgcaccacc aatcatggaa gcaactaatg gttttttcag cttcaaggtt actccataga
     4081 gtgaaggctc agtaattccc attaaagcag tgatcccagt ggacacagct agctgcttca
     4141 actctttgtt tttagttttc gcggctacag caaaggtagc acctgattga gcaatattag
     4201 aagccagcat tcctggccca ttgatcattt cagaacccgt actgctcaat tgcatagtag
     4261 cgattggcgt cattgcccaa gctgtacccg taaccaccaa aaatggctgc aaaccaccca
     4321 tcaaaaatgg aattaaccaa gctgctttgc cgttaatcca ttcagccccc attgctataa
     4381 gatcgttcag caacgttcca aacggtccaa tgacaaccaa cgctaaaggc cccataatta
     4441 aaaataccag catcggctta gtaaagaatt taataaacga aggcgaaatt cgatccgcaa
     4501 agggctctac ataggacatg atccaaacag taaagataat cggtaaaact gatcccgcat
     4561 aatctgcagc taataccgga attcctagaa aagaaacggc atcgccagta gaaaaaagct
     4621 gtgaaatatt tggatgaagc aaaatgccag caacagtaat cgccaaaatt gggttgacat
     4681 caaactttat ggctgcccca taggctaata gtactggcat aaaataaaag gctgcatcag
     4741 caatagtatt caaaatgatg taagtttgac tactgtctga tagtacacca gtcaaagtca
     4801 agacggccaa aatggctttt accatcccgg cacctgttat agctggaatc atcggtgtaa
     4861 aggtggtaga aatgacacta atcaatcttg aaataatact ttcttgattg tctgaagagg
     4921 ggcccttttt aattgaagtt cccacttcct tttgaatttc cttgaaggct gtttggactt
     4981 cattgccaat caccacttgg tattgtccac cgctatccac cacactaatt acaccaagtg
     5041 ttttttctaa ggctttcgta tcggctttcc ttttatcttt gagttcaaaa cgcagtctgg
     5101 tggcacaatg ggttaagctg accacatttt cttttccccc aacctgttgc aatacatttc
     5161 ttcctacttc tctgtaatcc attattctct ctccttaaag cagtaaaaaa gaccaagtca
     5221 aaccacgccc caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg cataatcagt
     5281 aacatgccaa taacgttatt ttgctgtaat ccaataatag cacattatga tagcgcaatc
     5341 aacagggaat ttaatttttt atcttttcgt ttgaccgtat agggtaatat aacagttaga
     5401 tcacatcaac tagggtatat ctgcgtatac ttactgctaa aataagtata tctaatatta
     5461 actataccag taaaaccatt cctacataac cagcgggaaa ttcccaaaat cgtgaaagca
     5521 tcaaaaatgc atataacgga tcacacaact tcaacaattc tgctcttggg aaaaattgat
     5581 gaatttggtg agtaaaggat ggaataaaca gcattccaac aatataggca acaatagtga
     5641 tacttgtttt tttggtaaaa atcagtaagc aatagccaat ttcaatcatt ccgagatata
     5701 aaggtatcaa tagaatgatt tgggctagta ttcttaaaaa ttaactagca aatgtttaaa
     5761 ctgatttatt tctaa
//
LOCUS       DAXWLF010000154         5743 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXWLF010000154 DAXWLF010000000
VERSION     DAXWLF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.157"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            141..1037
                     /locus_tag="ACGE4U_002301"
     CDS             141..1037
                     /locus_tag="ACGE4U_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFF8394411.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1187..2128)
                     /locus_tag="ACGE4U_002302"
     CDS             complement(1187..2128)
                     /locus_tag="ACGE4U_002302"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8394412.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2254..3375
                     /locus_tag="ACGE4U_002303"
     CDS             2254..3375
                     /locus_tag="ACGE4U_002303"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFF8394413.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3633..5135
                     /gene="eat(A)"
                     /locus_tag="ACGE4U_002304"
     CDS             3633..5135
                     /gene="eat(A)"
                     /locus_tag="ACGE4U_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFF8394414.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5202..5729
                     /locus_tag="ACGE4U_002305"
     CDS             5202..5729
                     /locus_tag="ACGE4U_002305"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8394415.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1883 a         1017 c         1212 g         1631 t
ORIGIN      
        1 taatagaaga atgatttatt tcatcatatt ttcatacaaa tgttaaattt tcatacaaat
       61 ataggaaacg ttttactggc tttttactat ttaaacaggc tatcatagga aaaagtgaca
      121 gagaaagtga ggcggaaaag atgaacaaaa atgacgtact gctagttgca aaaaataaat
      181 cactttattt tttgccttcc atccgctctt tagatgtttg gtccggaaaa gatttactac
      241 agtcggaatt cattaaatca gctagacaac agttggcaaa ttatcagctg atcgtttttt
      301 tagattatgg atttgatatg gagatggctc gacgcgtgcg cccatttacc aatgcaaaaa
      361 ttgtgttatt tttttggaat cacttcaaag cagaacacga ggcgatgctg aagcaggcac
      421 aagatgaacc ggcaatcgat gagatttatc attttgattt tttggaagca aaagaacttg
      481 ggctgaaaca taatagttca ttttattcga aacatatgaa tttttcttct agcgaaccaa
      541 agacggatct attttttggt gcaacgaata atgggagaaa agaacgagca gaaagctata
      601 agcaggaatt tataaaaaga gatgttacga ccaactattt cattcttcca catcgaggga
      661 atgaacaacc tggatatctt acttatgaag agtacctgaa aaaaacaggg gaatcgcgcg
      721 ggattctgga actgttgcgg gagggtcagc aaggagtgac tttgcggaca tttgaatcca
      781 tgtattttca aaagaaacta gtgacggata atcaagcgat tgctcattat catttttatc
      841 gtcctgaaaa tatttttttg cttcgagaac gtgatttaga tgaattaccg gaatttctgc
      901 atacaccatt taagccaatc ctatcagagc tattggaatt ttatgatgca gaaaattggg
      961 caaaacgatt tatcactaac gattccacta tttatgagca atacgaatat gacctctcct
     1021 ctgttgaaaa caaataaaaa acctacattt tgaagagatt atgtactgaa acaaacaagc
     1081 acaattctta tggcatcatg agagttgtgt tttctttttg atcttttatt gggcttaaaa
     1141 aaagagaacc agctgctgat tcagctgatt ctcatcagag agccttttat tctgcgccaa
     1201 ggattccttc ttttgcatgg actaattggg tcaaaaaggc acaatatggt gtagcaatat
     1261 catatttttg tccttttcgc caaatggcac cattgatgta gtcgatttca gtcaaacgat
     1321 ggtttttgat caggtcttgg tacatagatg ggtaatgcaa cccgatgttg tcaggatcat
     1381 aagtagaagc aatatgttgg tacacttctt cttgatctag tatgattcct tcttttgctg
     1441 cgatagaagc aaattcagat acgattgttc gaaccatgct ctctgccgct ttttgcgcgc
     1501 ctagttcagc aatattacaa tccaaaatcg tgcataaacc attcaatgtt ccatttacac
     1561 aagcttttcg ccaaatggaa taacgaacgt tatggctata gatcggattt aaatttgctt
     1621 cttgaaagac ttctacaact ttttttgtaa atgcttcgcc ttctggttcc aagttctcta
     1681 gttcgatttc tccatcacct aacaatgtga cttttccggg accagctaga ccagctgtcc
     1741 acatcgtgat cccaaataag atatttttct ttggcacata tttttccagt acatcttcat
     1801 gacctaatcc atttagtaaa cacaaaacat aagtattgtc agtaataatg gattggatcg
     1861 aacaaaacat atcatctaat tgattggctt ttgttaatgc tacgatcaag tccacgtgtt
     1921 cgttgctttc gatgatttct tctggtgaat agattggcag ttttgcgacc acttctttac
     1981 cgttaaagtc agcaatcaac ccatttgtac gaatggcttc aatgtgagca ggccaacgat
     2041 cgatcaagag gacctcattt ccgctttgat gtagcatcaa tccgattcgg ctgcccattg
     2101 ctccggcccc agctattgcg attttcatgt tttttctctc ctttattcaa tgagttccct
     2161 tttagtatat agaaaaatgg aagaaaatag cattgttttg tttgaaaaaa atgaaaaaaa
     2221 gtgtcttact taagaagaaa gtgagggata cagatggata ttttggcggc aattgattca
     2281 tttaaaggaa gtgcttcttc atttgaacta aatcaggcag ttctaaatgg ctgggaagat
     2341 cgtaaaggaa gaaaaatcaa tgtgccgatt gcagatggcg gagaaaatac tgcagaagca
     2401 atctattacg cgctcagggg aacatggcgt accattgatg ggatggatct cttattccgt
     2461 cagcgaaaaa tcagctattt attgacaact tggcaaggaa aaaacaccgc agtcatcgaa
     2521 tctgcacaag cattggggtt agatctactt gataaaccaa cggatcaaac tatccgactt
     2581 gcttccagtt atgccttagg agaacttatt aaagatgcac tgcttcaaga agtagaacaa
     2641 attattgtca ctttaggagg aagcgggtgt agcgatggtg gtctaggatt actccaatcc
     2701 ttgggtgctt ctttagaagg gtacacagaa ggaaatcctt tattatctac taaaaaactt
     2761 cattttgatc gaagaaacgt tcttgtttct aaagtaaaat tgattatcgc tgcagatgtt
     2821 accaatccat atacaggtca acaaggagct gcttggcaat ttggaagaca aaaaggagga
     2881 atagatacga cgctagcttt cttagataaa caagcaggaa agattgctca gcaaataaaa
     2941 gatttctggg aggtggattt agatgatatt ccaggaagtg gagcagcagg cggtattggt
     3001 ggagcccttt ttttgttggg agcgaagatg gtttctggtt tcgatttagt cagccaaatc
     3061 actggattgg aaaaaaagat cgaacaagca gatgtcatca ttacaggaga agggcggatc
     3121 gatcagcaaa cgatccacgg aaaagttcca tacggtgtag cagtgcttgc aaaaaaatat
     3181 cgaaaaccaa taatcgctat ttgcggaagt agagaccaag agctagaagg actttctgat
     3241 tttctcccaa ttgtcttaag tattcatttg ggtccaatag gtttagaaga agcaatggaa
     3301 cacaggcaaa cattagaaaa aacaaaaatc ttaggacaga cacttagcag atttttcacc
     3361 atcctcccga agtagttgac aaaatcattt tctttatgct atcgtaattt caaatatagg
     3421 acaaagtgag ggatccgtgc attgaaaaat taagtcgatt gatgaaagct gcattcagac
     3481 tgtaccttgt ggagacaagg aagcagtgtg cagattttcg ggattggctt ggttttttcg
     3541 atgtatgggt ctttttgtgt gtatccaacc cttgcatcct ctttagccag ttctgaaaat
     3601 aggactggtc tttttttacg aggtgatgga aaatgtctaa aatcgaaata aaaaatctga
     3661 cattcggcta cgacagccaa ggcacattat tatttgaaca agcaaatcta aattttgaca
     3721 cacaatggaa actaggactt atcggacgaa acggtcgagg aaagacaact ttactgaata
     3781 ttctacaaaa caaactacct tatcaagggc aagtaatcca tcagcaagaa tttgcctatt
     3841 tcccgcaaca gacaaaagat aaagaacgtt taacctatta cgtgttaaat gatattacgg
     3901 attttgagat atgggaaatc gaaagagagc tccaattgat gcaaacagat cctgaaatct
     3961 tatggagaga attcagcaca ctatcggggg gagagaagac aaaagtccta ctggcacttt
     4021 tatttgtgga tgacactcat ttcccgttaa tcgatgaacc aacgaatcat ttggatatct
     4081 ctggtagaaa acaagtagcg gcttatttga aaaagaaaaa acaaggcttc atcgtggtca
     4141 gccatgaccg gggatttatc gatgaagtag tggaccatgt tttagcaatc gaaaaaagtc
     4201 aactggaact ttatcaaggg aatttctcta tctatgaaga acagaaaaaa cttcgtgatg
     4261 aatttgaaat ggctcaaaat gaaaaattga aaaaagaagt cagtaggcta aagaaaacag
     4321 cagctgaaaa agccgaatgg tctcgttccc gagaaggaga taaaacaaag aaacaagtcg
     4381 gattcatcga tactgaatct agacgagtga ataaaggagc agtgggtgct gatgctgcac
     4441 ggacgatgaa acgatccaaa gcaatcgtga atcggatgga gacccagatc agcgagaaag
     4501 aaaaactatt aaaagatatc gaatatatcg attctttgac gatgaatagc caagcgtctc
     4561 accataagcg acttttaagc gtagaagatc ttcaattagg gtatgaaaat ctgttattcg
     4621 agccaattca ttttacaatc gagcctcatc agcgggtggc gatttcaggt cctaacggtg
     4681 caggaaagtc atccattatc cattatcttc tgggggcatt caacggcaag gttataggag
     4741 aaaaatacca gccaaaacat ctgagcatta gttatgcaag ccaaaattat gaagacaatc
     4801 gaggaacgtt ggcggaattt gcagagaaaa accaagtaga ctaccaagca tttttgaaca
     4861 acctccgaaa gcttgggatg gaaagagatg tttttcataa caagatcgag cagatgagta
     4921 tgggccaacg gaaaaaagtg gaattggcta aatctttatc acagccagct gaactatata
     4981 catgggatga accattgaat tatttggatg tcttcaatca agaacaatta gaacaactga
     5041 tcttgaacgt gaaacctgcc atgttactag tggaacatga tcaaaccttc ctggataaag
     5101 tatctactga gattatttct cttgagagaa tctaaccctt gtaatctcaa gaacaacaag
     5161 gtatggttaa ggggaacttt gccaacgaag gagagaatca catgctttat gtaacgagac
     5221 acggagaaac aacttggaac gcacaaggat tagtttgcgg acgggcagat gtgccgctga
     5281 ctgaaaaagg acagatgcag gcacagaaac tagcagaaaa ggtagtagat ttgcctgttc
     5341 caatcacgaa aatcatccat tcccctcttc aacgagcaag agatacagca caagcagttg
     5401 ctgatcgtct cagcttgccg ctgaccgttg atgaacgatt ggtagaaatg gattttgggg
     5461 attatgatgg actgccaagc aaagatgaga actttcaaaa agcacgttta gcgttcgctg
     5521 tacgattccc aaatggggag tcagtcttag atgtgtatgc acggatcgtt cctttattga
     5581 aagaatgcat cgaagacgaa gaaaatgtct atctgttagt ttgccataat gccttgataa
     5641 gagtgatcaa tgcttatttt catccgatgc ctaatgaggg gttctttaca ttcatggtag
     5701 ataatacaga attggtttct tatgagtaat tatgtttttg tgt
//
LOCUS       DAXWLF010000155         5723 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXWLF010000155 DAXWLF010000000
VERSION     DAXWLF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.158"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..268)
                     /locus_tag="ACGE4U_002306"
     CDS             complement(<1..268)
                     /locus_tag="ACGE4U_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFF8394416.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVID"
     gene            complement(523..843)
                     /locus_tag="ACGE4U_002307"
     CDS             complement(523..843)
                     /locus_tag="ACGE4U_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394417.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(876..1724)
                     /locus_tag="ACGE4U_002308"
     CDS             complement(876..1724)
                     /locus_tag="ACGE4U_002308"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394418.1"
                     /translation="MIEYDFNEAYSRIMRNYPLPSNTYLKAKYNTDQAILRAINRPKV
                     RPYENLKTYMFFLVNFEGTAKEGTYANPKSQSMFADYGENPKTANEPIWLSEIELTLI
                     LDYYDIKEMEVLDSHTFLTKKGLLKEYFERIEPLKTDPDTLKLYKNLRTKVFGAIGQK
                     SLREGETSTFDKYSMYNRAYSSMVAGVFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRS
                     EVPEFEELYKLGVVKKKVHTISKEEVEKALEFKNKGFENKGSFGKSKDEVKGVVIDDD
                     FEFDFD"
     gene            complement(2023..2817)
                     /locus_tag="ACGE4U_002309"
     CDS             complement(2023..2817)
                     /locus_tag="ACGE4U_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394419.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(2827..3702)
                     /locus_tag="ACGE4U_002310"
     CDS             complement(2827..3702)
                     /locus_tag="ACGE4U_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394420.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(3919..4176)
                     /locus_tag="ACGE4U_002311"
     CDS             complement(3919..4176)
                     /locus_tag="ACGE4U_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394421.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(4179..4403)
                     /locus_tag="ACGE4U_002312"
     CDS             complement(4179..4403)
                     /locus_tag="ACGE4U_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394422.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDTLELIEDIAS"
     gene            complement(4576..5619)
                     /locus_tag="ACGE4U_002313"
     CDS             complement(4576..5619)
                     /locus_tag="ACGE4U_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8394423.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         1579 a         1214 c          738 g         2192 t
ORIGIN      
        1 catcaataac tgtagcaata tattgagaga tagaaatatt atcttcactt ctatgtcgct
       61 ctataagatt atatatatca gggctgacat gaaacgtact tctctttttt gtttgccttt
      121 ttctaactgt tacgaccact tttggaactt cactatctcc attgatctct ttgtattttt
      181 caataaataa ttttctagct tgttctcgac tttttgccgg aatttcatct acaattttat
      241 cttctaaata aatatagtgt gttctcatac attcgtcctc tatcttcttt actctcaatc
      301 ttcatgatct tagttcttac cttgtttact cttaaacatt gcaaaaaaat ctagtcaaaa
      361 tcaaacttga aatcatcgtc tatcacaaca cccttaactt catctttaga tttcccaaag
      421 gaacccttat tttcaaatcc tttatttttg aatgatgatg gttatggaaa acttcctttg
      481 aaattctgac aagcataact agtcagaact gttttactta gattaagctt tcaaaagata
      541 ctgatgaaga actaagttct cagtaccttc ccattcttca ataaaaaata tttctccttc
      601 ttttacccac tctatagcta atttcttata atcaaataaa ccatatgtgt ttaagccttt
      661 gtctttcatt aaactcaaaa actgatcccc ttttaatctt tctgtatcat ccatcagaaa
      721 ttcaactaaa tctttatcca tgcgtgcaac tttttcgtct tttagtgcca ttgatttagc
      781 atagtcagtt gaatctttta gaataataac acctacattt ccttccacaa ttttcttctt
      841 catatttacc tcctaccatc ttttccaaag agtatttagt caaaatcaaa ctcgaaatca
      901 tcgtctatca caacaccctt aacttcatct ttagatttcc caaaggagcc cttattttca
      961 aatcctttgt ttttaaattc taaagctttt tcgacttctt ctttggatat tgtatgaact
     1021 tttttcttaa ctactccaag tttatacagc tcttcaaact ctggtacttc ggatctgaaa
     1081 tacaatccat cagtacgaat ttttaatagc ccatatgcgc tatgaacgta tttctgttca
     1141 tatctaacca tacgatctct aaatacgcct gctaccatgg aggaatacgc tcgattatac
     1201 atactgtatt tatcgaaggt gcttgtttct ccttctctta atgatttttg tccgatagca
     1261 ccaaatactt tcgttctaag atttttataa agcttcaacg tatcaggatc agtttttaat
     1321 ggctcgattc tttcaaaata ttctttgagt aatccttttt tcgtcaaaaa tgtgtggcta
     1381 tctaggactt ccatctcttt aatatcataa taatcaagga taagtgttag ctcaatctcc
     1441 gataaccaaa taggctcatt agctgttttg ggattttcgc cataatccgc aaacatcgac
     1501 tgtgattttg gattagcata tgttccttct ttggctgttc cttcaaaatt aaccaaaaag
     1561 aacatatacg tttttaaatt ttcatacggt cttacttttg gtctatttat cgctctcaaa
     1621 atagcttgat ctgtattata ttttgctttc aaataagtgt ttgacggcaa aggataattt
     1681 ctcatgatcc tcgaataggc ttcgttgaaa tcatattcga tcatctttgc tccttcctca
     1741 aacaaatcca agttatacat gctaccggag gtactggctc tataggctga acttggaaca
     1801 aagaaaggtg catcttccgg cttatcatag cccaaaaaat gtattcctgt ttgcttgttc
     1861 tgacggtaaa tgtccatcaa aagatatttg atcgtactaa aagaatctaa ttttcctctt
     1921 gtcatccacc ctaatccgac aagtctttta attttattct gcatttcagg tgtgtatatg
     1981 ctattgtttc ccatttatct gctcatctcc ggctttttta ttctagaccc tatatttgtt
     2041 cattacacca cttatctcgt ctacaaaggc tcccagatcg tgtgtaaagg catcatagta
     2101 atcttcaatc tcactcgatt cagttctccg gtttcttccg tttttatttc tgaatagata
     2161 catctgcgtt tctcttaaat cccttgcttt tttcgcaagt gctaataaat ccgtaaaatt
     2221 atcaataacc ataatgtcat tgatcataaa accttcttca tgaccatact tttcagccat
     2281 ctcgataaaa tcattaacat cgaacaactc caataattct gctttatatt tctctaacaa
     2341 tgtatttaaa tagcgatttc tcaattccca tgaactttca ccagttccgt attcaacctt
     2401 gatccctaaa ttcttagtgt agccttttgc tgtgtatcgt ccaatgaagt ctcccttatt
     2461 ccgattgcta tcttcttctt tgaaaaactc acgccaaatc ccctttttct tcttaggaat
     2521 tagacctctg ctcttgaaat catcatattt ttttgaagct tctcttcgtg tatctaagta
     2581 cctttgcttc agctctgtag ctgattcatt cgttccatat tcaacagtga tccttaagcg
     2641 cctgctataa ccttttgctg tatatcgtcc aacaaagtca ccgccacttc gatcactact
     2701 gacttcctta acaaattctc tccatatacc ttttttgtcc tttggtatta atcctcgtct
     2761 tttcatatct tgatattttt cagaagcttc ttttagagta ttcttttttg ctaccatata
     2821 ttccactcac tctttgttat ctagtatttt aataactggt ttcatttttg taatatcttc
     2881 tttttcagct atatacaact ccgttacggt aacgatctta gtatgattta gattaattac
     2941 tttcacttga tcgccaattt tgatttttcc tcgcaaccgt ttgggaataa accagttgta
     3001 taatcgtgat ttattattat ctacataagc ttttattgca ttttcaggaa acaggttatt
     3061 tagtctttca atttttttac ttttaaaaaa ttcattcgca aacttttcat caacaactaa
     3121 attaaataaa gtttcagaat ttacagcttc catatctatt cctttgtaga taggagaaag
     3181 caatttttta tattttgcca gtaatgactt tccagaatta ctaatcttaa tttggttact
     3241 taaatcttct tgataaagaa ccccatatcg aagtaacgcc ttaatataat gttttaatat
     3301 gccgttgata ccagaataaa tatccatgcc gttctcaata tagtagaaag tgtcgtctct
     3361 ccaaaacaat ttgaagctat tattcaaata aagtaacaag atcaatatat gtttgtcaga
     3421 aattctgaca aatctattac tttttagata ctcaccaatg gcttcactta actctttgaa
     3481 gccattgtat tttcccgata aaaatgtcgt aacattatct tctctctttt tcctatggtg
     3541 tgaacttacc ctgaaataat agagtctatt ctcatttaat attgaaaaat aaatactttc
     3601 agttgtttgt gagcattgta tatagttgaa atgatatcca tctggcatga cttttttaac
     3661 tgtattttgt actagctctg accacctttt gttagtagtc atatattttt ttccattaaa
     3721 ccaaacataa atatcttttt taaaaatttt gttccccccc tattgcgttt tctttgacat
     3781 taaagccctt tgttttcatc acattcgtat agccctagcc actcataacc tgatgctttt
     3841 gcgaagttta tttaaaagtg ttttcctttg taatctacct ttcccctcgt cctggtactt
     3901 ttcttggcga ctattaaact attcaacttt tttagaatca ttgtcagctt gaaatccttc
     3961 acgtttcatt atgtcttctg cttttccaat gctgattcct ttccaatcac gccatcctac
     4021 gcttccctta ttttgacgat aggctttaat agctgattcg gatatttgcc atcttgtagc
     4081 caactctttg gctgtgtact tatttgaaaa aagtagcttc tctatcagtg aaatatcaat
     4141 cattttattc tcactcctga aatcttcatt aatcatctct agctagctat atcttcaatc
     4201 aattctaatg tgtcggataa accatcaaca gcttcttcaa gttgatcaac ggattcttct
     4261 gctttttcat atctttctga tccctgtaga tttttaggta tattttcttt gtactcttct
     4321 tcttgaatta cttctaactc atcctttagc tcggaaagtc gctcgcttat ttcttctaac
     4381 ctttttcttc ttctttggtt catcaaaatc acctcttcgc ataatctcta cgattttaat
     4441 ataccacttt tgtgtactac attcaatatt ttttgttata gattcgtatt acaaaaattt
     4501 cctctaaaaa aataagagcc tatttaccat ttcagtaatt aagctcttat ttcatttagt
     4561 tgataattct atcaattaac gtacagcaac cgccattcca gtcacattaa atgctctctc
     4621 atacgtgctt gcattgatcc attttgcgcc aaatacaggg tcaacaacat ggtatgcacc
     4681 atttctgaag ccatccacga gaactacatg tccattccat actgcacgac cccaaggcat
     4741 ttgtttgaac tctggcgtag catagttata agtaatccaa gttacaaccg gattaccatt
     4801 gcgaatttca ttcttgattc cattcgttcc tgaaccagtg atattgacgg cattaccgcc
     4861 aacgtttttt gcccatttaa ctactgggtc agccatcatg ccttgatacg ttccattaac
     4921 gttatgacgc cactctcctg agtaaccatg ataaggatta ttgtcattag cttttggcat
     4981 atagtttaca aaactggtta agctttgatt tgtggctaca cctttgtagt gtaaaccttc
     5041 taataaagaa gctccttcgc ataacattgt atttccttgc catacaacag gaacgttcaa
     5101 cactttttca gtgcttggag caggtgtagg atttggtacg ctgtttggca ttgcatacca
     5161 gcctgtacct tcaatcttcc aaccttgttt actaattacg ttcttttcat tttgatctaa
     5221 cgtgtagtga tgccaattca cgtatttgtt atataaacgt gttacaggaa ctctatttgc
     5281 tccgttgtag gaatagaacg caacaccttc gtacgtccat cctgtgtgtg aaatcttatc
     5341 tctttctccg gcatcaactg tgtaaaaatg ttcaccgtta taccgattat acaaacgata
     5401 aacaggagta ccacttgttg gagcttgcca tgcgacacct tcgtaatgcc atccataacg
     5461 tttcagattg tctctttctc caacgcttct agtgtataga tgctctccgc tattgggatt
     5521 atacatacga tacatggaaa tagagctttc tgctgaaact gaaggtgata ataatactag
     5581 ccccattgtg aatagaattc ctactaaaaa ttttttcatt tgattttacc ccttttcatt
     5641 ttttattgtc attattgcat gaaaattatt cgtaagattg ccaattacat aaacgattgt
     5701 tttaagatca taatcgttgt tgt
//
LOCUS       DAXWLF010000156         5667 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXWLF010000156 DAXWLF010000000
VERSION     DAXWLF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.159"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            40..978
                     /locus_tag="ACGE4U_002314"
     CDS             40..978
                     /locus_tag="ACGE4U_002314"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFF8394424.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1230..2240
                     /locus_tag="ACGE4U_002315"
     CDS             1230..2240
                     /locus_tag="ACGE4U_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFF8394425.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
     gene            2608..4032
                     /locus_tag="ACGE4U_002316"
     CDS             2608..4032
                     /locus_tag="ACGE4U_002316"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFF8394426.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            4118..4546
                     /locus_tag="ACGE4U_002317"
     CDS             4118..4546
                     /locus_tag="ACGE4U_002317"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFF8394427.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            4634..>5667
                     /locus_tag="ACGE4U_002318"
     CDS             4634..>5667
                     /locus_tag="ACGE4U_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFF8394428.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEF"
BASE COUNT         1976 a          743 c         1048 g         1900 t
ORIGIN      
        1 taagaataaa tagcacttaa aaatagagat gagggattta tgggaagaca agaattatat
       61 agtttagcta tcaatgaatt ggaaaaaaga aatcttgaaa tacatgaatt aaatcaaaaa
      121 aataattcta ttttaggcat gaaaatacga aataaaatta atcaacttaa ccaaatgaaa
      181 aaacgagata atctgttgcc agcaaaatat acccttaaat cgatgaccgg taaggggcct
      241 attaaagact attcaaacag agagaatcgc agtgatgaac taagaaaaaa agagttaact
      301 aatcaaggaa cacctaaaag gattgctgtg tatagttgta tcactggtaa ttatgattct
      361 attaaagaac cggatttttc tcatccattg gtagattact atttgtatac agataatgtg
      421 attacaagtg agcattggaa tattagagag atacctaaat atatatcgga attaaaaaat
      481 aatattttga tcaatagata tattaaattt catcctaatg agctatttac tgactacgat
      541 tttactttgt atgttgatgg taatgtaaca ataacttctg atattaccgg attactgaac
      601 agttcttcca aacttgggtt aagtatgcat aaacatagtg aaagaaaatc tttatattct
      661 gaggcagatg cattaattgc agttggcaaa ggtaataagg aaagtattat agagcaaatt
      721 agtaagtaca gagaagaagg atttcctgat aattttggat tggctgaagc cacggttatt
      781 ttagttgatt taaataattc aatcgctgag aatcttctag atttgtggtg gagagagtat
      841 ttgaactcag aaagtgggcg agatcagctc gcatttcctt acgtgttgtg gaagtccgga
      901 catgcaatga aggaagtgac taccttaggc aataatgtat ataaaagcgc taaattaaga
      961 atcataagtc attattagcc ctaaatgatt tctaaactat tctttcaaat cctagtaaat
     1021 atttggagat agaagatttc ctttttaaag tcataaaaaa ttaagttata ttttttgagt
     1081 gaatggagaa gacgcatagt atcataaact tcgttatatc ttaaattatg gggtatgcct
     1141 aaataataaa tgtaatacga gtataaagaa gaaagcaatt ttgggtttaa ccctattggt
     1201 tcctttcaga aaaaggtgga tgaataaaaa tgaaaatatt aataacaggt ggagcaggat
     1261 ttattggttt tcatttgtct agaaaattac tcaaggaaac tgaaaatcaa attgtagcga
     1321 ttgataatct caatgattat tatgatgtcc aattaaaaga agatcgatta acgatactgt
     1381 acgattataa taattttaaa tttatcagaa tggatctatt agagaaagaa aaattagatt
     1441 ctctatttga aagtgaaggt tttgacatag taattcattt agctgctcaa gcaggtgtta
     1501 gatattctat tgaaaaccct tatgcttata ttgattctaa tattatcggt tttcttaata
     1561 ttcttgaagc ctgccgaaac ttccctgtaa agcatctatt atatgcatca tctagttcag
     1621 tatatggtgg gaacacgaaa gtaccgtttt caacagaaga ttcggtggat catccagtta
     1681 gcctttatgc ggctaccaaa aaatcgaatg aattaatggc tcatacttat agccaccttt
     1741 ataaaatacc aacaactggg ctgagatttt tcactgtcta tggtccattt gggcgaccag
     1801 atatggccta tttctctttt accaagaata ttttatctgg aaaagaaatt aagattttta
     1861 accatggaaa gatggaaaga gatttcactt acatcgatga tatcgtagag ggaatcacta
     1921 gattaatcaa taaagtacct gaacccaact ccgactggac agacatcgat gatttgagta
     1981 gtgaaagttg ggcaccatat aagatataca atattggaaa taataagccg gttgcattgt
     2041 tggagtttgt tgaaacaatt gaaaagtatt taggtgttaa agcgaacaag atttttttgg
     2101 acatgcagcc gggtgatgtc gttagaactt atgccgatat tgaagattta gaggaaaaaa
     2161 tcaactttaa accgaatata accttggatg agggcattaa aaaattcgtt gaatggtata
     2221 cagtgtatta tttgagttaa ttttgaaagg aatattgaag atgggtaatt catacgattt
     2281 ttttagcttt gttaggtatt ttaggaaatt ctactttcaa acgtgacgaa ctttctcgcc
     2341 gaatagatat tttctataac taaaagaatt tctataaaac aaaattgtgt atcaagatgt
     2401 gtgaactgga ttctgactgc caatattgat tgatgagatt gaagaaatat atagttgatc
     2461 atggatatct ttatttgaaa agatcttagc tattcacata ttcaaaaaaa tttttactac
     2521 gaggactcac acgatacaga ggaatttagg tagttctaat taaaccagaa agtcatctat
     2581 agatgtgagt tgagggaggt catacggttg gatagtagca gtaataaatc acattcaata
     2641 gctaaaaaag cttttattta cgcaatagga acattatcat caaaaatgat atctgtcatt
     2701 ataatcccta tctatgcagt atatatttct gttgccgatt taggaagctt tgattatcaa
     2761 aatacaattg ctcaatttct ttctccagtt atttttggag caatatggga agcaatttta
     2821 cgcatgggat taggcaaaga aaaaaatatt cagaaaaaag tagtgacgaa cagtatttgt
     2881 tttgttatag caaatacagt gatattagcc atttttttac caataatata tagaatgatt
     2941 ttcccgaatt tagagtatta tcaattgttt gtttttcaat taattttcac tcctatccta
     3001 agtgtttttc aatatattgt aagagcacta ggtgaatcaa agatttttgt gatgagtggt
     3061 ataatcgctg cgggtatcaa cctgttactc cttactattt ttgttgttac aatgggtatg
     3121 gggatacttg gtctcttact ctctatcata atttctaatg ttataaattt gatttttttg
     3181 attttcaggg ggcagatctt tcaatatttc gcaacgagtt cttttgattt tttattttta
     3241 aagtccatgc tgaaatactc tatcccactc actttgaatc tcacttttgg atggctaata
     3301 gttggttttt caagatttta tattggaacg tacatcggag taacagaaaa tggtattttt
     3361 tcttttgcga tgaaattttc atctataatt ttaactctta caagtattgt tagtatgtct
     3421 atgatagagg atgcggttat cacgtctgag aatgatgatt tcatcgaaag attcgttgag
     3481 aaaaatacct atatttttaa aatgtacatt aggttagcga ctctttctat tcctatcatt
     3541 tgctgtgcat actcttttat aaccaaccct gattataaaa aatcattagt tctagttcca
     3601 atattagtta ttatggcatg tttcagtggg atgacaacaa atatcggaaa tatatttgca
     3661 gtatacaata aaacaaatca gttattaatg atttcagtag tgtcaggtct attgagtgtt
     3721 gttggcacct atttattagg ctattctttt ggagtaattg gtgtctcatt tgctactttg
     3781 ctcggtactt tagtattatt tggacttaga tattattttg gaaggaaaat tgcttattat
     3841 gcatttgatt ggaagcgaat atcgctactt ttagttctct attccgcaat cgcttttgta
     3901 tgcattcata gaggcttcta ttttaatctt ttcttaacga ttatttttag tgctattttc
     3961 gtttttacgt ataaacaatt aattataacg tatctaaaaa aatttttcag aataaaaatg
     4021 gctaaaaggt gagggtatac gaagtaagaa tagaatcttt cgaagaataa agagaaaact
     4081 atttggaaaa aatctgcgag aatgtattaa tgaaggtttg atattcggaa aaaaacatag
     4141 tttagtagag gttcctgaat ttgggagcga gccatattta attgaaatag gggataattt
     4201 cagatgttca tttggtgtta aatttataac acacgatggt ggagtctggg tcttaaggaa
     4261 gatgagttct gtgagcgagt caacttcaaa aataggtagg atcaaattgg gaaataatat
     4321 ttttattggt atttactcta ttgttttccc aggagtttca attggcgata atgtaattat
     4381 tagagcgaac actttagtaa ataaagatat cccaaataat agtgttgtag cgggtagttc
     4441 atgtaaagtg atttcaacta tagaggagca ttatgagaaa aataaaaatg aatttattaa
     4501 atatcgaacc tcgatgattt tgaagcaaaa aaattttttc tattaaataa tattgattga
     4561 ataaggaata tgttcaaaag gataacagct aaaatactat aaaatgaaat ttgattggag
     4621 cgtatggaag aatatgaaaa tttcagtttt cggattaggc tatgttggtc tagcaaattc
     4681 actattatta gcacaaaaag agcaggtcaa agcttatgat atcgtggaag aaaaaatcac
     4741 gatgttacaa aatggcatct caccaataga agataaagaa gttcatgaat ttttaaaacg
     4801 ggatgactta aatgtcgaat ttacgtcaaa ttttactgat gcagtaaact tcggtgatta
     4861 cttgattatt gcaactccaa cagattatga cgaaaagaaa aactatttta atacatcaac
     4921 agtagaagat gtaattgaga aggctttagc aattcgtcca gattcaacat tcattattaa
     4981 gtcgacagtt ccagttggct atgcaaatga aattaaagcg aaatttggta cagaaaacat
     5041 cattttttca cctgaatttt tgcgtgaagg aaaagctttg tacgacaact tatatccatc
     5101 aagaattata gttggagaat gttctgctcg tggtcgcgag attgcaacta tgtttcaaag
     5161 aaacgcattt aacaaagatg catcagttct tttgactcac tcaactgaag cagaagctat
     5221 taaattattc tcgaatacat atttggcatt gcgagtggcg tatttcaatg aattagatac
     5281 gtatgcagaa tttcgtgggc ttgatagtaa gcaaattatt gaaggtgtcg gtttggatcc
     5341 acggatcggt tcgcattata ataatccttc ttttggctat ggtggatact gtttacccaa
     5401 agacacaaaa caattaagag ccaactatga aaaagtacca agcaatatca tcagtgcaat
     5461 tgttgatgca aatacaacaa ggaaagattt tattgctgga cagattttgg cgaaaaaacc
     5521 aaatgttgtt ggaatctatc gtttaacgat gaaggccggt tcagataatt ttcgatattc
     5581 gtcgatccaa ggaattatga aacggattaa agccaaagga attgaagtgg ttgtttacga
     5641 gcctacattg gatgaagatg aatttta
//
LOCUS       DAXWLF010000157         5662 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXWLF010000157 DAXWLF010000000
VERSION     DAXWLF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.160"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            163..2223
                     /locus_tag="ACGE4U_002319"
     CDS             163..2223
                     /locus_tag="ACGE4U_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFF8394429.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2306..2875
                     /locus_tag="ACGE4U_002320"
     CDS             2306..2875
                     /locus_tag="ACGE4U_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8394430.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3050..3856)
                     /locus_tag="ACGE4U_002321"
     CDS             complement(3050..3856)
                     /locus_tag="ACGE4U_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394431.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3994..5508
                     /locus_tag="ACGE4U_002322"
     CDS             3994..5508
                     /locus_tag="ACGE4U_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFF8394432.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2017 a          980 c         1139 g         1526 t
ORIGIN      
        1 aaaagtacat aagttattat tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact
       61 ctcctaatag tccatcttct tttgatttta tttatataat gaaatcatct tatgaaaatt
      121 actacttcat gtttagacaa gagtagggag gactaagaat aaatgtgttc aaaaagacgt
      181 tcaaaacaac taaataaaca cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa
      241 cactacgtta agtttatgat gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg
      301 catgtacccg ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc tgtttctgag
      361 accactgatt cccctatcac tattactcat actttcactg atgcaaatgg aaaacagcta
      421 cttcctgcat tgacttccta taataaagtc agttatcctc caacgataaa ccactactgg
      481 ttaattccta acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc gctttctatc
      541 ttttccgata tgactgatcc tacaaaaggt gatcagttaa aacagatcac tgatattttg
      601 aatcatgctt tgacagatat aaaggacggt ggaagcgcac aagtttctta tgtatatgaa
      661 aaagattatt cacagtttga aagaaagaaa atcgatgttc tggcagggac aaaagtttcg
      721 atatctgatc tggtagaaag gctgaagaac gcagatggaa cacaaggaga tatgtccaaa
      781 gtaatttcaa acaatgggaa tatcgtggat acgactaata caggaacatc tactatttcc
      841 ttgtcctatt ttgatccaat tgctttaaaa acaatgactg catctgctgt gatccatgtt
      901 cgtgctgatc aaacagctat caaaggaaaa gatcttcact taagaacagg agagagttac
      961 gcccctaaag atcttgttga atatgtaaca aaaagcgatg gaacttcagg aaataaagaa
     1021 gaaatcaaaa tgaacgggac agtagatact acaactccgg gagaatatcc cgttgtatta
     1081 acttatatag actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa
     1141 gcatctgctg cttcatcgac tgtcatcact cattttatag atagtcaaac aaataaagaa
     1201 cttcaagcag aaacacatat gagtagtgta aatcgtattc ctgatttgcg cctacctgct
     1261 gaattttctg atcgagtgcc aaatatagat ttatcgactt atgaacaaaa tgggaaagtc
     1321 acttctattt ctgattggat gcaagcaaac ggattagaca gaaaaagtca ctggtcggag
     1381 atccttgaag tacttcactc agaactgatg aataatagga agcaatcagg aggggaaatt
     1441 acctttactt atgtttatat accagacaat cgttcattag aaggtcatga tcttattgtt
     1501 catccaaacg aaaaaataaa caaagaagag ttgatccaat cagcaacaaa ttccttaggt
     1561 aaacccgtac cgttaaagga tgtatcggtt acaatcaatg aagtaccatt ggaggatggt
     1621 tatttgacca aacaaacagg tgattttacg attatgtata gttatcatga cccgtattca
     1681 ctccaagaac tccatgctca gtcactatta catgttttac cattaaacaa acagatagat
     1741 caaaaagaac aaaaaagaca agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat
     1801 gcagtttctc caacagggaa agtaaaacaa acaagaagcc tagagttagc aagaatatct
     1861 agaaaaatct cagaatctaa taaaacgata aggcaagaag ctcatttggc cccacaagtt
     1921 tctggattgg aaaatgaaat tttagagcct aaaataaaac aacaatcaga aaaagaattt
     1981 gttgctaaac agtcggaaga tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa
     2041 cgcttgtcaa ctattgattc caccgatgag catctgcagc cgcaagtacc acttcctagt
     2101 ccgggaacac ccgcacctgg tggaggtatt ccttcttctg ctttaggaga tttcatgcga
     2161 ggtatggcag gaacatgggt atacgctaat cgaaatgaat ttgacgcaga tgactggttc
     2221 tgatttatat ctctatctgt tatagtcaaa taagtagctg agtcatatgt tatttggcat
     2281 aatggagacg aggagggata gaaaaatgtt agataaaacg ttaccttacg cagaaatctg
     2341 gatgacaaga ccacttcaac aaaatgttcc aatcacgcct ttagcggaag gttttgaatt
     2401 tcgattttat caaaaagaag atgaatctgc ttgggcggag attgagactt ccgttgggga
     2461 atttcaaacg acagatgaag cacttgagta ttttcaacaa aactttgctc cttatcctga
     2521 agaacttgaa agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg
     2581 taccgcttgg cgaaaaaaat caagagagca agtgtatccg gtttttcatt ggttagcagt
     2641 gaaaccagat taccaaggaa aagggctcgc aaaagcattg actgccagag tcttgcaaga
     2701 atttcctacc ctccatacgg acggaccaat ctatcttcac acacagacat ggagccatca
     2761 ggcgattgct ctttataaaa aaatgggttt tacttttatt gctgaaaatc tagacggcac
     2821 acctaatcca gattatgaaa aagttatgca ggttttaagt aaattagata aataacgaaa
     2881 tggtgaaata agtcctcagt attgggataa aaaacgaaat agtcattcct catatcttac
     2941 ttttttgagt taatactatc ggataactaa tggcatgcca tacattcaaa aaagaagggg
     3001 cagtgacaaa agtattgtca ctgccccttc tcttgttttt tagcatgttt tatcgtttga
     3061 tttttttagg aataaaattc ttgatgaacg taaatttttg gtaatcgttt cgactataga
     3121 ggtaaccaaa tacactgaaa acaactgctc caataaaatt aacaaataaa tccttcatag
     3181 tatccatgat tcccacgtct aagtaaccat tttggatgat tgttttatct gttttaccat
     3241 ctgcattttg actttcaatg attgtccgag tgatatctgg aacatgatag acagtgtttc
     3301 cgtctttact taactccaca ctattgattt gctggatgat cgtatctttt tgcatatcga
     3361 attgcagtaa tttatctgct gaaaattcca caaactccca cataacacca actgtcatcg
     3421 aaaaacagaa ggaaacaact gctagtaata aaggagtcaa gttcatggtt tttgtatttt
     3481 tattgagcaa ataaacaagg gaaaagccga cacccccagc taaaaatccg ctgaatgcat
     3541 gtagaaaagt atcccacatt gaaaaatagc cataaaaatc acttaactct cctaaaactg
     3601 ttgaactaaa aataaaagat aacaaaatga tttctagcat atttggaatc tgtatcttta
     3661 atatcttttc cagaaaataa gggatttcaa ataaaattaa tgtcagtaat aaaagcattg
     3721 tatttcccca gtgttggttc cagacctgat ccactagcat aataacgacg atgatacgta
     3781 ataaaatata gattccgttt gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg
     3841 ttttcaaatg attcataaaa taactccttc atgtatatcc ttaattatac taagaagcgt
     3901 gtgtttacca aagctatatg acttaaagaa aaacagagtt gatattgtga aaaaaatcac
     3961 ttaatatagt ataaggagaa aagggggata caaatgaaaa aattagttgc tggtttatta
     4021 acagccgttg cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag
     4081 acaagttcta gtacgaagga atctgccgaa gtatcatcag gcgcttctga acaagtatat
     4141 acagatccaa aagaaatgaa agaatcttat gatgtcgtga ttattggagc tggtggtgca
     4201 gggatggcag cagctatcga agcaaaagat gctgggttga atccagtaat tttggaaaag
     4261 atgcctgttg ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca
     4321 aaatttcaaa aagaagaagg aatcaaagac tcgaatgatg ccttctatga agaaacgcta
     4381 aaaggtggtg gaggaacgaa tgataaagaa atgcttcgat actttgtcga tcactctgct
     4441 gatgcggtcg attggctaga tcaaaacggt atcaaactgg acaacttaac gatcactgga
     4501 gggatgagtg aaaagaggac acatcgtccg tctgatggct cggctattgg tggatactta
     4561 gtggaggggt tattgaataa cgtccatgag cgggaaatcc ctatttttgt caacgcagat
     4621 gtgacagaca ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca tgttcaagga
     4681 gaagaaccaa aagaggtcac cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc
     4741 agcaaagaat ttattgaaaa atatcgtccc gatttagctg attatattac gacaaatcaa
     4801 gaaggatcaa ctggagatgg tatcaccatg attgaaaaat taggtggaca aaccgttgat
     4861 atggataaaa tacaaattca tccaactgtg cagcaggatg agggggtctt gattggtgag
     4921 gttgttcgcg gagaaggagc aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa
     4981 atggatacaa gggataaagt atctgcagca attacagcat tgcctgaaaa atcagcttat
     5041 ttgatttttg accaaggcgt gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa
     5101 ggatatgtca caactggaaa gacaatggaa gaattagcag aaaaaatcga tttaccaaaa
     5161 gaaacactag caaaaacagt tgatggatgg aatacggatg ttgcggagaa aaaagatgct
     5221 caattcaacc gtgccacagc catggaacat gacttatcgt ctccgaatta ttatgcgatc
     5281 aaaatcgcac caggaatcca ttacacgatg ggcggtgtga aaatcaatac gaacactgaa
     5341 gtattagata aagaaaacca gccaattagg gggttatatg ctgctggtga agtcacaggt
     5401 ggcttacatg gaaacaaccg gatcggtgga aattcagttg cggagatcat tatttttggg
     5461 cgacaagcgg gcatacaagc agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa
     5521 ataaaaagag gttgtgaaat cagcgttttg agctaatacc gagggtcagc tgattgaaca
     5581 ctgtttagtg aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt
     5641 aaaaacagct tcttatgttt tt
//
LOCUS       DAXWLF010000158         5561 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXWLF010000158 DAXWLF010000000
VERSION     DAXWLF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.161"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..720
                     /locus_tag="ACGE4U_002323"
     CDS             <1..720
                     /locus_tag="ACGE4U_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394433.1"
                     /translation="IIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSALA
                     NQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNKQE
                     LINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVLKT
                     TELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLILS
                     LFPFTFFIHHLQSRRPGRNET"
     gene            710..1219
                     /locus_tag="ACGE4U_002324"
     CDS             710..1219
                     /locus_tag="ACGE4U_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394434.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1289..2737)
                     /locus_tag="ACGE4U_002325"
     CDS             complement(1289..2737)
                     /locus_tag="ACGE4U_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8394435.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2737..3453)
                     /locus_tag="ACGE4U_002326"
     CDS             complement(2737..3453)
                     /locus_tag="ACGE4U_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFF8394436.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3434..3784)
                     /locus_tag="ACGE4U_002327"
     CDS             complement(3434..3784)
                     /locus_tag="ACGE4U_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFF8394437.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3928..4701
                     /locus_tag="ACGE4U_002328"
     CDS             3928..4701
                     /locus_tag="ACGE4U_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8394438.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            4872..5238
                     /gene="ssrA"
                     /locus_tag="ACGE4U_002329"
     tmRNA           4872..5238
                     /gene="ssrA"
                     /locus_tag="ACGE4U_002329"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5370..>5561
                     /locus_tag="ACGE4U_002330"
     CDS             5370..>5561
                     /locus_tag="ACGE4U_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394439.1"
                     /translation="MAFKKTSFNIYVPSILPTLFLLYKKNISSYTLFEVNIMLMEGKT
                     QLWFKFDPSNRFIKDFYKVW"
BASE COUNT         1600 a         1102 c          973 g         1886 t
ORIGIN      
        1 ataatccgct atgctactgc tcgattaatt tattacaaaa agcagacaat actctattgt
       61 ttattttcaa gcttttctgc ttttttattg atagcatgcc tgactcttta ccattcgcaa
      121 tcggctttag ccaatcaagt ccaagatcgg ctttcattta ttggcgcttc tgcatctaca
      181 gagcttcttc cggctttaga attagctagc cgattttata taaaaggcat attcagttta
      241 ttttttctat ttgccttcct ttttatcttt ttcttttact attcagtcag gcaaaacaag
      301 caggaattga tcaattggcg cctgactggt ctttctaaaa ccaaattatt cctttttatt
      361 ttctggcagc tgttctttcc tctctttctt tgttgtatct tggttttatt gtggatcgtc
      421 agcttccagt cagtttatga agcgcttctt caaaggatca attttcttgt cttaaagact
      481 actgaactgc cagatatccg tgatatcttg acttcggtaa gcggacttac aattccgatg
      541 gatcaacaga ctttttttac catcgatttc caaaatgatc tttttttcac agatatactt
      601 aagggatttg cccaaactct tattgggctc agtggatgct gtctcattct tagtctattc
      661 cctttcactt tttttatcca tcatttgcaa agtaggaggc ctggacgtaa tgaaacctga
      721 atatttgatt catgctgaac acttgaccgt tattttgtgt cctctcgaaa aacagcataa
      781 aatattggag cacttgaaaa aagaggttct tgttgcaaaa ctggataatt ttgcgatcat
      841 ccaaaaaaat tggccgatgt ttccttatct gaatcttaaa gaccatgtcc tgttggatgt
      901 accggaaaaa gaaatcaaac aagatcgttt ggcctatcaa gaaaaacttg atatctctcc
      961 ttccttgctt aattgtgcgg cagctgagtt gacgtctttt gaaaaagtaa agctgcaatt
     1021 acttcatgcc ttgctttcca agcgaaacaa tattgttatt gaagatacat ttgatgaact
     1081 tagtgtattg gagatccaag agctactcca cttgctttct tatttagctc atgaagaaaa
     1141 tcaaggtatc ttactcttta ctcatgatgc tactattgct caaagtcctt atattgatcg
     1201 tttagagccc gcgggctaaa tcaatcattt tatagtaaaa acaagcggta tagaagatcg
     1261 attcatgatc ttttataccg cttgtttttt actctttttt gatttctacg tcaggcaaac
     1321 gaacagcgaa ttttgttcct tttcctagtt cgctttctac ttctaatttt cctttatgca
     1381 agcgtacaag ttgctgcacg attggtaaac ctaatcctga ttcaccatat tttgtatttt
     1441 ttcttgaagg atctacttta taataacgat cccagatact tttcatttgt tgttcagaca
     1501 ttccaattcc cgtatcttca atcgtgatga tcgtttcaag atagcctttt tctaaagtaa
     1561 tcgtgatttg gccgttttca gtaaactgta tcgcgttttg aataatattg accatgattt
     1621 ggacaaatcg gtcataatca gcgtaaacat caatatcatc aacggctttc aatgtcaatg
     1681 tatctcctgc tgcttctgct ttagcttcta actgtgcaac aatatttttc aatgtttctg
     1741 ttccattgaa ttttttaatg accatactga tttgattcgt acggattttt tcataatcca
     1801 agttttcatt caccaaacgg atcagacgct ctgtttcatt tttcatcaat ttaatcgcgt
     1861 tttctcgctg attttcaggg attgcattgt attctaaccc ttccagaagt ccgttgattg
     1921 tcgtaagagg tgttctcatc tcgtgagaag catctgccat aaattgtctt cgacgatttt
     1981 cttgttcttc aatttctgct tgagattctt tcaatgaatt cgtcatttta ttgaaatcat
     2041 ctgctaactc atcaaattca tctttgtcat gtacggggag ctgtacatca aaattaccat
     2101 tcgtcacttc tttcgtcgca ttccttaacc gattgatacg cttaacttgg aatgctgcaa
     2161 aagcatagct ggcaataact gcaacgatac tggaaaaaat aaatcctttg aacaggttca
     2221 gtgtaattgg acgaacgctg ttatcgatat ttcgcgcagg ttgactaact acaagtgctc
     2281 catagaattc atgattcaaa ttaaatggca caagtgcgta agaggtcgcc tgtgcttgtc
     2341 ctaaaatatt tttattcgat gtgaattttt cttgacgtcc attcctcaga tttttccatt
     2401 gatcagctgt taaggtaaaa ttcagtaaag ctgtattagt cggataaatg acacgttcgt
     2461 ttttatcgat aaatataaag ttcacatttt gttctgtcag tacttgctct gtcagcagca
     2521 atgcattttg aaacgcctga tcttgactaa actgcgggag cgtatcggca taggtttgcg
     2581 ttgtcttttc aactgattca gcatatccaa acagttgctg gtaattgttt tcttcaatcg
     2641 tttgttttgt cagttgtgta aaagaaactc caacgatcaa taagatcaac gcgaccattc
     2701 cgcaaaaagc taagagttgc tgatacaaat aacgcattag gcaacaccag aatcatcgaa
     2761 tttgtagccg actccccaaa cagtttggat gacttgtggt ccgacctttt cgattttttg
     2821 tcgtaatttc ttgatatggg cgtccactgt ccgttcatca ccaaagtact gataatccca
     2881 gactaattca agcaactgct cccgagagaa tacttgacgt ggtttttttg ctaaagtaaa
     2941 caatagatcg aactctttag gcgtcagtcc ttcaattggc cggttgtcca gatacgcttc
     3001 tcgtgttttc gtattcatct tgaaatgact agtctcgata tcaaaatgat catctgaaac
     3061 tgctgcttct tctcttgctt ctgccaactc actacggcga tgcaaagctt tcattcgagc
     3121 aataagcgtc agaggactga aaggttttgt gacataatca tctgccccca tctctaagcc
     3181 gatcacctga tcgctttcag aatctctagc tgtcagcatg ataatcggca cagtacttga
     3241 aactttgcgg atctctcttc ctactagcat accgtccagt gtgggcaaat tcaggtctag
     3301 tgtaatcata tcccaatgtt gtgggttttc taaaaaggca tcgagccctt cttttccatc
     3361 atacttgaaa gttgcttccc aaccttcatt taagaaaaac atctgcatca tttcagaaac
     3421 agattgatta tcttcaatca ttaaaatatt cattatcgtt ccctccagtt tccaggtcat
     3481 atttctgtaa tttcttttct gtcataataa aaataggcat aattggaaat actagcagaa
     3541 gcatttcaat cagaaaataa ttcgtatgcc cagatgtctt taaataatag ccgatcaatg
     3601 ccatcagtaa gcccataaga aaaaaccagt ttccgcttgt cttttgagcc agccgccaat
     3661 gagcgtctgt ttttgcggca agaggcgaat gatacccata aaaaggcaga tttccttttg
     3721 agggcagaat ccgaaaaaga ctgccaatta ttaagaataa gataccaata ctgaaaaaaa
     3781 tcattctttc cctcctaaat tctcttctta tagtatactc taaattgttg actgaaccaa
     3841 tatgttcagg aattcttcac gaaaggtttg tcttttgttc attctttgtt aagataaact
     3901 tattccaata aaaaaggagt tttatttgtg tcaaattatc gtgcaatcac tttctttgat
     3961 cttgatggaa cattactaga tgcgaaatca aaaatcacac ccgaagtggc aaaagctatg
     4021 aacgcattaa aagaaaacaa tgttctcccc gtcatcgcta caggacgtac agaagcagaa
     4081 atccactcta tcatggaaga tgcgggcatt acttctgcgg tcaccatgaa tggttctttc
     4141 attcgagtag acggcgaaga aatctattct caaacatttt cttcagaaga atgtcaaaaa
     4201 atgtatgatc atgtacagtt gaacggtcat gaactttctt actataacga tcagaaaatc
     4261 tggtgtaccg gtcatagcca aacaatgatc aatgcttacg acttcattca ttcagatgtt
     4321 ccggaaattg atcctttagg ctttaaagaa aacacagtaa atatgttgct gatcttatct
     4381 caatcaggtg acgaatacta tcacgaacat tttcctgagc taacttttta tcggaatggt
     4441 ccttactcga tcgatacagt gaagaaagga atctcaaaag gggcaggtgt caaacgtctg
     4501 caaaaagaac ttcagctgga aaaagttcct acttttggat ttggcgatgg tccgaatgat
     4561 tttgccttgt tggaagcttg cgacaacaaa atcgctatgg acaacgccta tgacggatta
     4621 aaagaacttt ctacctttat cacaaagaaa aatacagaag gcggaattgt ccacgcattg
     4681 aagtattttg atctgattta agatcttttt ttgaagctac ctcttttctt aagaaaatcg
     4741 cttatttttt cttaagaaag gggcagttat tatataaata atgggatatg attcgctttt
     4801 ttattgaatt tatcccaata tatgctatac taattttgta tgaaaaatac acgtaaccct
     4861 tgttagctaa cggggacgtt acggattcga caggcacagt cgagcttgaa ttgcgtttcg
     4921 taggttacgt ctacgtaaaa acgttacagt taaatataac tgctaaaaac gaaaacaact
     4981 cttacgcttt agctgcctaa aaacagttag cgtagatcct ctcggcatcg cccatgtgct
     5041 cgagtaaggg tcctaacttt agtgggatac gtttcaactt tccgtctgta agttgaaaaa
     5101 gagaacatca gactagcgat acagaatgcc tgtcactcgg caagctgtaa agtgaatcct
     5161 taaatgagtt gactatgaac gtagattttt aagtggcgat gtgtttggac gcgggttcga
     5221 ctcccgccgt ctccattgtg agatttcgcc agatttcgct agatgcttaa atccttattt
     5281 tataagggtt tgagcatttt ttgttttcgc tgattttcgt taaatgaatg aaaatggcat
     5341 tcaaaatggc attcaaaatg gcattcaaaa tggcattcaa aaaaacatct tttaatatat
     5401 atgttccatc catattgcca acgctattcc tgctttacaa aaagaacatt agttcgtata
     5461 ctctttttga ggtgaacatt atgttgatgg aaggaaagac acaattatgg tttaaatttg
     5521 atccttcgaa tagatttatc aaagattttt ataaagtatg g
//
LOCUS       DAXWLF010000159         5530 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXWLF010000159 DAXWLF010000000
VERSION     DAXWLF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.162"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            19..837
                     /gene="purR"
                     /locus_tag="ACGE4U_002331"
     CDS             19..837
                     /gene="purR"
                     /locus_tag="ACGE4U_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFF8394440.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1340..2713
                     /gene="glmU"
                     /locus_tag="ACGE4U_002332"
     CDS             1340..2713
                     /gene="glmU"
                     /locus_tag="ACGE4U_002332"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFF8394441.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2807..3778
                     /locus_tag="ACGE4U_002333"
     CDS             2807..3778
                     /locus_tag="ACGE4U_002333"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFF8394442.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3784..3886
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3952..4521
                     /gene="thiT"
                     /locus_tag="ACGE4U_002334"
     CDS             3952..4521
                     /gene="thiT"
                     /locus_tag="ACGE4U_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFF8394443.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4602..5375)
                     /locus_tag="ACGE4U_002335"
     CDS             complement(4602..5375)
                     /locus_tag="ACGE4U_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394444.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1803 a          977 c         1163 g         1587 t
ORIGIN      
        1 taaaaggagt tggaataggt gaaagtacgt cgcagtgaac gtctagttga catgacacag
       61 tatttattga atcacccgca tgaattgatt tccttaacaa gctttgccga acgttatgaa
      121 tctgctaaat cttctatcag tgaagatttg ggaatcatca agaaaacatt taaagaacga
      181 gggtatggaa ccttggaaac aatcccgggt gctgcaggtg gtgtattgtt catccctgaa
      241 atttcatatg aagaggcgaa aaaatatatt gaatctctag cagaacgtct atcagaacaa
      301 gaccgtctgc ttccaggagg atatgtttat ctctctgatt tgttaggaga acctgaactg
      361 ctgcgtcaag ttggaagaat cattgcttct aaataccttg gtgaaaaaat cgatgctgta
      421 atgactgtag ccacaaaagg cgtacctatt gcccaagctg tgtcctatta tctgaatgcg
      481 ccattcgtta ttgtacgccg ggactcaaaa ataacagaag gatcaacagt aagtgtcaac
      541 tatgtgtctg gatcttctga aagaattgaa aagatggaac tgtctaagag aagtttgaaa
      601 cgtggttcac gtgtccttgt agtggatgat ttcatgaaag gcggcggaac agtcaacgga
      661 atgaagagct tgattgaaga attcgaatca gaacttgttg gcatcactgt atttgccgaa
      721 tcaaaattca acggtcatcg cgccatcgat gattatacgt cactgcttta tgtcaaagat
      781 gtagatactc aaacgaaaaa tattacggtt gtgccaggaa attatttttc agaataagag
      841 gttgtgaaaa agccgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca
      901 cattttttca taaatttggc ttaataccga gaaactggct tttgaacacc gtttatctag
      961 aggttgtgaa atcagcgtcc tgtcctgagt attagctcaa taaacggaaa aattgctcct
     1021 catattttgc cgtttattga gctaataccg aaggtcagct gattgaaaaa cgtttaatga
     1081 agaggttgta agcctaccgt ttaactctaa gtcacaagaa acgatttcaa aaaacagctt
     1141 ttcatgtttt atgagtagtg tgacttgtga ccgaggagtt ggcaggagag caccggttac
     1201 caaatagata ggcccgttat ggtctatcta tttttttatc cagaaaacgc tgggataaaa
     1261 gaaaatcact tgttttgttg aaaaaatggg tatactggga taaactgaaa tcagcaaact
     1321 atatttagga gtgacgcagt tggacgcacg ttatgcaatt attttggcgg cagggaaagg
     1381 tacccgcatg aagtcaaaat tatataaagt tttacatccc gtttctggta aacctatggt
     1441 agaacatatc attaatcggg tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg
     1501 acacggagca gaacaagtta aagcacaact aggagaacgc agtaaatatg cgttacaagc
     1561 agaacaattg ggaacaggac atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa
     1621 aggaacaacg ttagttatca gtggcgatac accattattg actactgaaa cgctgaacaa
     1681 tctttttgaa tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga
     1741 aaacccaaca ggatatggac gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt
     1801 ggaacaaaaa gatgcaacac cagaagaagc tcttgtacaa gaaatcaata caggaactta
     1861 ctgttttgac aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa acaatgctca
     1921 aggtgaatac tatctaacag atatcatcga aatcttaaaa gaagaaggcc atacagttgc
     1981 tgcttatcaa acggatgatt ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc
     2041 taaagcaaat gagatcatga gaaagcgaat taaccagatg catatggtca atggcgtaag
     2101 ctttgtcgat tcagcaacga catacatcga tgcaggcgta gaaatcggtc cagacacatt
     2161 gatcgaagca ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg
     2221 gtcacattca aaaatcgtag acagtaggat tgaggatcac gtcgtcattg aaaactcagt
     2281 cattgaaagc agccatgtca agaaacatgc agatgtggga ccttatgcac atttacgacc
     2341 aaaagctgaa attggcgaaa acgtccatat cggaaacttt gtggaagtga aaaatgctca
     2401 aatcggcaaa ggaactaaag tcggtcactt gacttatgtt ggtgatgcta cgctgggaga
     2461 agaaatcaat gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga acaaacatca
     2521 tacgactgtc ggcgaccaca gctttattgg ttctagcacg aatatcatcg gacctgtcga
     2581 ggtagctaaa aacagttcga ttgctgccgg gtcaacaatt acagacaata ttccagaata
     2641 tgcgctggca attgcgagag caagacaagt caataaagaa ggatatgcaa aaaaactccc
     2701 ttatctaaac taagactttt gcaaaatcac ttgatttatt taaaaaatat gactactatt
     2761 tattaggtag aattctagag ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta
     2821 ctttgaccca agattaaaaa tttttgcgtt aaattccaat cgcccattag cagagaaaat
     2881 tgcagcagca gtcggcgtcg agttaggaaa atcatctgtt actcaattta gtgatggaga
     2941 aattcaagta aacattgaag aaagtattcg tggatcgcat gtctatgtta ttcaatcaac
     3001 gagcagccca gtcaatgata acttgatgga gttgctgatc atgattgacg cactgaaacg
     3061 agcaagtgca aaaacaatca acgtagtcat gccttattat ggctatgcgc gtcaagaccg
     3121 taaagcacgt gcacgggaac caatcacagc gaaattagta gcaaacatga ttcaaaaagc
     3181 tggtgcaaca agaatgctga ctttggacct tcatgcagtc caaatccaag gtttctttga
     3241 tattccagtc gatcatttga tgggtgcgcc acttattgcc aattatttct tagaaaaagg
     3301 aatcaaaggc gatgatgttg tcgttgtttc accagaccat ggtggtgtca cacgggcacg
     3361 taaattagcg gaatttttga aatctccgat tgcgatcata gacaaacgcc gtccaaaagc
     3421 aaatgttgca gaagtaatga atatcattgg acaagtcaaa ggcaaaactt gtgtactgat
     3481 cgatgatatg atcgatacag caggtacgat cacattggct gccaatgctt tgaaagaagc
     3541 tggtgcaact agtgtgtatg cttcatgtac acatccagta ttatccggcc ctgctttaca
     3601 aagaatagaa gattcagcta tcgagcgtct agttgttaca gactcgatct atcttccaga
     3661 agatcgtaaa atcgacaaaa tcgatgaagt aagtgttggc ggactaatgg gagatgcaat
     3721 caaacgtatc catgaaaaca aaccagttag tccattgttt gaaacaaaaa ataaataata
     3781 aataaactca caaaggggag tccaattggg ctgagattga atctatttct aaacccttcg
     3841 tacctgtatc ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg actattttcg
     3901 aaagctgctt cctcctttgt gactttttta ttcaaaggag gaagtttttt tatgggaaaa
     3961 aatagagttt gggtagaagg aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt
     4021 ccgatccaga tcggcagcag tttttctatt tcattagggc agattccatt gactcttttt
     4081 gcgttaagaa gagggacaaa ggcagggatg ctggcaggtt tcatttgggg catcctacat
     4141 tttccgttgg gacaagttta ttatttaagt gtcgttcaag tgttgattga gtacccgatt
     4201 gcctatactt ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa aggctatgta
     4261 gacgataata cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt
     4321 tccagatatt tttggcattt tattgcagga gtggttttct ggggagcgta tgcactatgg
     4381 gggatgaatt cttggctctt ttctttcgta atgaatggtg ccagcgggtt gagtacagct
     4441 attgtaacga tcatcgtttt attagggatc caacaaacga ctccttcgat ttttgtgcca
     4501 gataaacgac aggagctgta aataaaaaag aagcaagaaa cttgagacag aatcatgtcc
     4561 tagtttcttg tttccttttt attttttgat aagaaataga tctacttcaa tggcagataa
     4621 gaaagatcgg catcttctac caattgccag tgtcgatcgg tgacagagta gattaaagta
     4681 gcgatgattt cctcttctcg ataccgggtg ccttttaatt tccaaatata atcttcaatc
     4741 acatagattg ggatacgata tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg
     4801 atcccttcct gcatcaaatt acgcatttct ttcaataatt ccacactgat tttttcaaca
     4861 ggtgtttctt ttttgaaaaa agattttttt acattgatct tatctgacaa gatcgaactt
     4921 aatttactag caaaaatcct ataaaattca tctaaatagc gataataaat ccgattcagc
     4981 tgatcattaa gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga
     5041 agatgtgttt tcatatgacc aaaatataat aattctgaaa tttctaaagg tgtcaattct
     5101 ttcagcatcg ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc acgcctgtta
     5161 cgagaagagg aaaagaaacg ctgaatattt tctgtaccac ggattacttt cagccctgta
     5221 tgggattcat attctccagc agaagcagat ggatcaagca agagcaagtt ttgaggaaaa
     5281 tgcacgatac tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac agcattactt
     5341 gtggtatcaa tatgtacata aactgactca gtcatcatgt tcctcctctt atttcctctt
     5401 aatctctttt attttacaac atttgcatta aaaaagcttg tttcttttat tttaaaattc
     5461 cattacaaaa tcacgttttc attgaaaaca ctctcatttt caattaattt ttgtgatttt
     5521 tgtatttttt
//
LOCUS       DAXWLF010000160         5503 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXWLF010000160 DAXWLF010000000
VERSION     DAXWLF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.163"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            39..974
                     /gene="pfkB"
                     /locus_tag="ACGE4U_002336"
     CDS             39..974
                     /gene="pfkB"
                     /locus_tag="ACGE4U_002336"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFF8394445.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            971..2416
                     /locus_tag="ACGE4U_002337"
     CDS             971..2416
                     /locus_tag="ACGE4U_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFF8394446.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            2444..2899
                     /locus_tag="ACGE4U_002338"
     CDS             2444..2899
                     /locus_tag="ACGE4U_002338"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFF8394447.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            2918..3892
                     /gene="lacD"
                     /locus_tag="ACGE4U_002339"
     CDS             2918..3892
                     /gene="lacD"
                     /locus_tag="ACGE4U_002339"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8394448.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(4003..4564)
                     /locus_tag="ACGE4U_002340"
                     /pseudo
     CDS             complement(4003..4564)
                     /locus_tag="ACGE4U_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            4708..5322
                     /locus_tag="ACGE4U_002341"
     CDS             4708..5322
                     /locus_tag="ACGE4U_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8394449.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
BASE COUNT         1753 a          886 c         1205 g         1659 t
ORIGIN      
        1 ttgatacata atgaatatgt aagaaaggag gagcgtaaat gtcagtttat acatgtactt
       61 taaacttggc aatcgacctt tttatcgaaa cagatgaaat gaaaccatcc caagtcaatc
      121 gaacaaacga tgatgatata caagctaatg gaaaaggtgt gaatgtttca ttgatcatga
      181 agcaattagg tatcgatagt acagctttag gatttaaagc aggttttacc gggaattata
      241 ttgaggatta tttgcatcaa aaagatatta gcactgattt tattagcgta ccgggaatga
      301 ctagaatcaa cgtttttaca caagttaata aaaccaatga agaattcaaa ttggttaata
      361 aaggacctga gattcctcag gaacaaatca aaacttttct aaatcaaata caaaagctgc
      421 aagctgacga ttatttgtgt gtttctggca gtttgccgcg aggggtctca cccgaaattc
      481 ttattgatat cagtcgtatt tgttttgaaa ataaggtcca tctgattatt gattcaagct
      541 atccggaaat aattgattgt ctgccgtatc agccgttctt attaaaacca aacgaggagg
      601 aattgcaatc ttggtataat gtcgagctta gtgatcgtga aggatatatt cattatgctg
      661 aacgcttgat tgccgatgga gcgaaaaatg ttctcctttc tctaggagga gagggaggaa
      721 tatttttaaa taaggatatc gtttattctg ggaattctcc taaaggtaaa gtagtcaata
      781 cagcgtgtgc tggtgacact ttgttgggaa catttcttgc aggatattta aaaggtgaac
      841 aggtagatat taatttgaag aaaagtttag cagctggaag ttcaaccgct tttcgtaaag
      901 gattaacaga tttttcggat gttgcagagt tggaaaaaca aatcgaaatc aaagagggag
      961 cgagtaaaaa atgacaactt acaaaattgt tgccgcaaca ggatgtccaa caggaattgc
     1021 ccacacttat atggctcaag aagcattgga gcaggcagct aagcgcaaag gggtatctat
     1081 caaagttgaa acccatggtc aagtagggat agagaatgaa ttaacaccag aagaaataaa
     1141 aaacgctgac gctgtaatca ttgcagctga caaagatgtt caagcagaac gtttcgcagg
     1201 taagcgtgta atcgatgtgc ctgtcagcaa ggggattaaa gatgctgacc aattgatcga
     1261 agatgcgttg gctggtaaag gttcaatcac tgcagaaaac aaaacagttg acgctgctgg
     1321 tgagaaagcg gagatggggg ttgcagcagg cattggtcac agtatttata aaaatctcat
     1381 gaacggtgtt tctcatatgc tgccgtttgt agtggcaggt ggggtaatta ttgcaatttc
     1441 gtttgccatc tggggtgtgt actcttttga tcccaatgat cctacttaca atccgactgc
     1501 cgctatgtta aagggtgttg gtgatgcggc aatgggaatg atggttccta ttttagcagc
     1561 ttatatcgct gaaggaatcg ctaaacgtcc tggtttggtt gttgggtttg ttggcggact
     1621 gttagccaat tctggcggag ccggtttctt aggcggaatt tttgcaggtt tcttagcagg
     1681 ctactttatc ttactattgc aaaaggtcat gaaaggcttg cctaaacaat tggatggtct
     1741 gaaggcgatc ttcttatatc cggtgatcgg tgtcttgttt gttggtgtag tcatgtcttt
     1801 attagcaaat ccaatggaag cagtcaacca aggaatgatg aatttcttgg caggattcca
     1861 aaattctaat ccattaattt tagggattat cgttggctgt atgtcagcat ttgatatggg
     1921 aggtccagtc aataaagcgg cttatgttac agggactgcg ttgttggcac aaggtaacca
     1981 tacatttatg gcaggggttt cggcagcttg tatcgcgccc ccattgatta ctggatttgc
     2041 cgcactgttt tttggaaaat atttcgacac gaatgatcgc aacgctggtt tagtcaactt
     2101 tatcttgggc tcaacgcaca ttaccgaagg agcaattcca tttgcggcta aagatccgct
     2161 gcgtgttctg ccaactatga tgctaggttc atcaattgct gctgtactga catatatgtt
     2221 tggtgtccaa gttcccgcac ctcatggagg ttttctagta ttacctgttg ttactggtaa
     2281 ggttgcatgg gtagggtcga ttttacttgg ttcaatcgtt ggcggagtaa ttttagggtt
     2341 gatccaaaaa cgtagtgtag aaaaacgtga agcagaagca gctgctgttg aaatgaaaga
     2401 agcaaccaac aactaaaggt tattctagaa agaaggattt caaatgactt tgatcaaaac
     2461 aaataaagtt tttatgaata aaaattttac gacgcaagat gaggtaatga acttccttgc
     2521 agatcagtca attgtcttga gcattagtga agaccgagat gcagtttacg aaaaattgat
     2581 ggaaagagaa gaagaaagca cgactggtat gatggatggt tttgccatcc ctcatgccaa
     2641 agatgctagt atcaaggatg ccgatattgt gattgttcgg ttaaacggta aggtcgattg
     2701 gaaaagttta gatggagaag gaacggattt tgtaatcgct ctgtttattc cagattcaga
     2761 ggctggaaca acccatctaa aatttttatc tcaggtagcc agacttttga tgcacaaaga
     2821 tgtgacagaa ggtctgaaac aagcccagtc tgctgaagaa atagctgaac ttttaaacca
     2881 aaaattagaa gaaaactaaa aaggaagtag tgaaatcatg cctttaacag aaaaaaagaa
     2941 aacttatatt cgtcaactaa gtaatgaaag tggcatcatc agtgcgttag caatcgatca
     3001 acgtggtgcc ttgaaaaaaa tgatcggtaa atttcaaaat aaatcagcaa ccgatgaaca
     3061 aatcatcaat ttcaaaaaaa tcatttcttc tgaattgact ccatatgctt cagcaatctt
     3121 attagatcca gaatatgggt tgccggcagc cgaagttcgc gataaaaatg ctggtctgtt
     3181 gttggcctat gaaaaaactg gttacgatgc ttcaactcca ggacgtttac cggatatttt
     3241 atctatttgg tctgtaaaac gtctaaagga gcaaggggcc gatgcttgta agttcttgtt
     3301 atactacgat gtagatgaat ctgatgacat caatgaccaa aagaaggcgt ttatggagcg
     3361 gattggttct gaatgtgttg ctgaagattt gccatttttc ttagaattgg tttcttatga
     3421 tgccaacgat cctgatactt cttcgaagga atatgccttg aaaaaaccgc ataaagtaaa
     3481 tgaaatgatg aaggaatttt ctaagcctcg ctatggagtt gacgttttga aagtggaagt
     3541 tcctgttaat atgaactatg tggatggtta cactaaaaat gaagttgctt ataccaaaga
     3601 agaagctgct gcttatttca aagaacaatc cgagactacc aacctgccat ttattttctt
     3661 gagcgcagga gtaactgctg aattgttcca agaaaccttg aaatttgcaa aagaggctgg
     3721 ctctacattc aatggggtac tctgtgggcg cgcaacttgg gcgaatggtg tagaaccatt
     3781 cgttactaaa ggtgaatatg ctgccaaaga atggatgcag acacaaggac gcaagaacat
     3841 cgaagagctg aataatgttt tgaaagaaac agcaacagct attaaattgt aagaaagctt
     3901 taactataac cttaagtttg taacctaatg atttttaata atcaatttga gcttttaaat
     3961 aaagaattgg aagcgtcccg acgcttcctt tttcgtttgt ttttaaggca tgataggttc
     4021 tatgttgtcg ttcaataagc gcaagaaggt cagcattttt ttcttagagt atgcaaaatt
     4081 gcttttctac ccaatgattt aaaacggaga ggcacccttg gatatggtag atggtgaagt
     4141 aacgcatttc ttctgtgatc acggtttttt tgtcttcttg tagccagatt tttaaataat
     4201 tttcttcgat cagattattt aatctctttt gaaattggat atctccgtgg ttgatgacga
     4261 ataacttgta tacgaacgta ttcgtataga catagtcgat caattgagcg tatagatcct
     4321 cgtagctatg tgtcgtatcg taggaaatga tttcatccaa gtcttggaaa aagctatttt
     4381 ccagtttttc ataaagatca tagatatccg tataatgtgt ataaaatgtc gaacggtgaa
     4441 gatcagcagt ttcggtcagt tcacggatgg tgatgttttg aattttttta tctactaata
     4501 attttgataa tgcttctgct aacgcttttt ctgttttttt gacgcggcga tcctcttttt
     4561 tcattttgca gcctcctaat caacataatc aaggaattgt gtcggttatc cgacaatttc
     4621 accgctatct gaacatagat aaccgacacc ttgtctatta taatataagt atagcatatc
     4681 aatatttaaa gaataggaga ataagtgatg gaattagcct ttaagcaact ggaaaagaaa
     4741 gactacaaaa aagccattca attcgcgatt aaaggtatgc actttgattg gtataccaag
     4801 aacaaactcc ttttgaatct ttatggaaga tacttttggt atctagaatt gaatcgcgcg
     4861 acacaaatca ttgctttata tgcggatgat gagttggcag gtgttttatt agcagacatc
     4921 aaaggaaaat caaaaaagca tcattcattt agtcaaaagt tatatgttaa actatttgat
     4981 ttcttacaaa atgcatttgt gaaagacagc aaaggcgggt atgacgacac caatgacgaa
     5041 ttgctttcag actatttaaa aaaacattcc ccagatggag aaattgtctt tttagctgcc
     5101 aaccctgatt taaagatcaa ggggatcggt agtaaattat taaaagaatt tgaacgccga
     5161 gagcaaggaa aagaagtatt tctgttcacg gatagcggct gtacgtatca attttatgaa
     5221 cgacgcggtt tcgaacggaa aaaagaaaaa gataacgtca tgaagatcct taataaagaa
     5281 gtagatctcc gctgtatgtt atatagtaag atcattcagt aatgtttttt actatcagaa
     5341 agcagaagta ccacgaaggt caatggaaaa agcgagtaat tgatccattt agatgaagat
     5401 aggtgtagtc tcaaagatat tagctggcaa agatatcccc agttaaaaat agatttctgt
     5461 atccaacaat aaaaatattt ttgtatttat tagtatatat act
//
LOCUS       DAXWLF010000161         5375 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXWLF010000161 DAXWLF010000000
VERSION     DAXWLF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.164"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(295..1920)
                     /gene="groL"
                     /locus_tag="ACGE4U_002342"
     CDS             complement(295..1920)
                     /gene="groL"
                     /locus_tag="ACGE4U_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFF8394450.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(1971..2255)
                     /gene="groES"
                     /locus_tag="ACGE4U_002343"
     CDS             complement(1971..2255)
                     /gene="groES"
                     /locus_tag="ACGE4U_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFF8394451.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            2480..3142
                     /locus_tag="ACGE4U_002344"
     CDS             2480..3142
                     /locus_tag="ACGE4U_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFF8394452.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(3218..4510)
                     /locus_tag="ACGE4U_002345"
     CDS             complement(3218..4510)
                     /locus_tag="ACGE4U_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8394453.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            4680..5309
                     /locus_tag="ACGE4U_002346"
     CDS             4680..5309
                     /locus_tag="ACGE4U_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8394454.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
BASE COUNT         1573 a         1137 c          946 g         1719 t
ORIGIN      
        1 atttactttt aatagataaa atattataac tattaaaaat aactattttc attataatta
       61 cacacaaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
      121 tataaacagc ggaagcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg
      181 aaaagcaatt tccgtgaatt tcttcttatt tcttggagtt aaacgcttct gttccagcct
      241 ctttttatct gtctttcact aatgatcagt tgtagataag caagttgctt tttcttacat
      301 cataccgccc atcattgatg gatccattgc aggagctgct ggcgctgctg gttctggttt
      361 atctgcaacg actgcttctg tcgttaataa caaggcagaa acggaagcgg cattttgcaa
      421 ggcagaacgt gttactttag ttgggtctac gatacctgct tcaaccatgt ttacccattc
      481 tccagtagca gcattgaaac cagtgcctag ttccacgttc ttcaatttat caacaatcac
      541 tgatccttca taaccagcgt tttcagcgat ttgacgaatt ggttcttcta aagcacgaac
      601 aacgatcttg atacctgttg ccacgtctcc ttctgcttct acagcagaaa ctttgctgat
      661 aacgttcact agagctgtac caccacctga gaccattcct tcttctactg cggcacgtgt
      721 agcattcaat gcatcttcaa tgcgtaattt taattctttc aattctgttt cagtagctgc
      781 accaacttta acaacagcta caccacctgc taatttagct aagcgttctt gtaatttttc
      841 gcggtcaaaa tcagaagttg tttcagcaat ttggtttttg atcaattgca cacgagcttc
      901 gattgcttct ttttctccag aaccttctac gatcgttgta ttgtctttat ctacaacaac
      961 tttagaagca tttcctaagt tttcgattgt tacatctttc aattctaaac ctagatcgtc
     1021 agtaattact gtaccacctg tcaaaatagc gatatcttca agcattgctt tacgacgatc
     1081 gccgaaacca ggagctttta cagctactac gttgaatgtt ccacggattt tgttcaatac
     1141 aagagttggc aatgcttcac catctacatc gtcagcaata atcaacaatg gacgtgattg
     1201 ttgcaagatt tgttctaaca atggcagaat atcttggatg ttagagattt ttttgtctgt
     1261 gatcaagata tatgggtttt ctagaacagc ttccattttg tcgttatctg ttaccatata
     1321 ttgtgataag taaccacgat caaactgcat accttctaca acatctagtt ctgtttcgat
     1381 cccttttgat tcttcgattg tgatgacacc gtcgtttcca actttttcca ttgcatcagc
     1441 aatcaaatgt ccaactttat cagaacccga agaaacagct gctacttgtg caatggcttc
     1501 ttttgaatca acaacagttg agatattatg caattcttcg actgctgctt ttgttgccaa
     1561 ttcgatcccg cgacgaatgc ctagaggatt agcacctgca gttacgtttt ttaaaccttc
     1621 gcgaacgatt gcttgtgtca gaacagttgc tgtcgttgta ccgtctcctg caatatcatt
     1681 tgtttttgat gctacttctg atacaagttt tgcacccatg ttttcaaaat gatcttctaa
     1741 ttcgatttct tttgcgatcg tcacaccatc atttgtgatc aatggagagc cgtaagattt
     1801 ttctagtacg acatttcggc ctttaggtcc taatgttact ttcactgtat ctgctaattt
     1861 atctaccccg cgtaacatag ctgcgcgtgc gtcttctgca aatttcaatt cttttgccat
     1921 gattgattca cctcattgat tatttttgat tattttttta tcttccaaat ttactcaaca
     1981 atcgccatga tatcttttcc tgcgacgatc agatattctt ttccttcgta ttttacttct
     2041 gtaccggcgt atttttcgaa cataacttgg tcgcctgctt ttacagcggc aggaactttt
     2101 tcaccgtttt ctaacaaacg gccttcaccg actgcaacaa ctgtcccagt ttggggtttc
     2161 tctttagcag cagaagctaa aacgattcct ccgacagttt tttcttcttc ttttgcgact
     2221 tcgataatga cgcgatcacc taatggtttt aacacgataa atccctccga taactctttt
     2281 tttaatctgt tagcactctt aaccttcgag tgctaaccta catttttaat aatactcatt
     2341 ctccctcttt tttgcaagct tttaaatcaa agaaaagaaa ttttattctg acagctgctt
     2401 attgaaatta tgatataatt gaaatcaatc gatataagct tgccttgcat gcttgttatc
     2461 ccttaaaagg aggaattaca tgtctttaaa aaaatatagc ttcctcagca ttttttgcta
     2521 tggtctaatt tttatctcac ccttgatcct agcttcgata ggacttgtaa aatctacgtc
     2581 tgaattgatc acagcaactg cagtcatgta tatactaggc gccgttgtcc tagctatttt
     2641 ttattttaag caacgtgaac ccttagcgat cgaatcagcg gtaaaaaaag cttctgctcc
     2701 aaagattgtt atttatggat tggttggaat ctttgtcgca ttgattctgc aaagcatcgc
     2761 tgtcatgtta gaatctgtat tgtttggcaa agcaacccct tccgagaata cacaaaatat
     2821 catccagatg atcatggaag cacccgcttt tattttggcg acaactgttg ctggacccat
     2881 catggaagag ttcgtattcc gccgcagtat actggggatc atcagtcgat attcaaattt
     2941 ttgggtcgga gcagtgatca gctctttgct gtttgcgttt gctcataatg atggtcatct
     3001 gctaatctac tttttcctag gtttcttctt cagcctagag tacaaagcaa ctggtcggat
     3061 ctggacatcg atgatcacac acgtaggaat gaacacatta gttgttcttg ttcaattagc
     3121 tgtccaaaaa gggctagtat aaacatgact ataaatactc ctgtcatcct agattggtga
     3181 ttttcccaat ctaaagcgac aggagtattt ttgtttttta gtcgttcatc ttttctgctg
     3241 ctccatcatt ttttgtctgt ctttcctttt tgcagtttgc agaatctatt tttttcaaaa
     3301 atgggaaata aatgaaaaaa ctaattgaca acacgatacc ttgtaaaatc gctgcaggca
     3361 ctccgcctac taagaaaccc gagaggatcg gcggtgttgt ccaagggact tggactgctg
     3421 taaataaagg aacgaatcca aaatataaag cggaataggt aatcaatcct gaaacgaccg
     3481 gtgcgaaaat aaaaggaaca gccatcatcg ggttcatcac gatcggtgtg gaaaaaataa
     3541 ttgattcatt gatgttgaaa aaggctggta aaagagcaag atttcctaat tctctgtatt
     3601 tcttcgattt cgcaaaaacc gtcataaaaa atacagcccc taacgtcaat ccagcacccg
     3661 taaccgtcat gaactgatcg agaaattgct gagtaacgat atgtccgcca tttgccaccg
     3721 tcaactcttg tcctgaggct aagatttctg cattcgctaa tgaattcgct tgcaagacgg
     3781 gactaaccaa cccattgacc atcgcagatc catgaagacc aaataaccat aaaaacggaa
     3841 caaaaaaacc taataaaagc acaccaaaca acgtgtctat gaacgattga atgggaagct
     3901 ggacatatgt ccatactcct tgaacaacag tcattctgaa caactgttga aaaaccgctg
     3961 aaccgataag cgtaaaagtg actataacaa tgattggaat caatgaagta aaagactgcc
     4021 aaacattttc aggtatcttc tctctttgct tccacccttt cgcaagtctt actaaacgtg
     4081 catagcccca tccgatgatg agcccagttg taactgctcc tagtatccct tcagcacctg
     4141 tccagacagt atctatctga gtatttagtg gctgaataag cagtagataa gcagacaagg
     4201 ctgttatgcc gactggtaag cgatcgatgt cttcttcttt tgcataagcg tatgcaatgg
     4261 caaaaacgat ccaaaaggct atcacatcaa atgtagcctg gtatacttgt gtaaaataca
     4321 tagaaaatcc tgttttgtct atccactgag cgatgtaatc cacaggaaaa tgagcaaata
     4381 agaaaaacag cgagccaaca ataattattg ggatcgtgta agtcattcca ttacgcaaag
     4441 cagttaaggg tctaagattc acaacagatg ttatcattgg taaaaaggca cgatctaacc
     4501 actctttcat ttcttatctc cttcaacttc tatttttatc aatcagtttt cttaatttaa
     4561 ctatacccaa tgatcacgaa ccatgaaagg aatgcgcact gagcttttca aaaatcggat
     4621 tctttgacga atattcagca actgcttaca atggacagga ataaaaaagg aggtttttta
     4681 tgaaaattgc actcattgga gctactggcc atgcaggttc atttatttta gacgaagcat
     4741 tgtccagaga tttagacgtg acggcaatcg tgcgtcatcc agaaaaattg ccatcagatg
     4801 ttccgtttat acaaaaggat ttatttgagt tgacaactac tgatcttgca tcttttgatg
     4861 ttgtcgttga tgctttcaac gctcccaaag gaaaagaaga aatgcatcaa acttctttgt
     4921 cccatttgat tgagatgctt gaaggaaccg atacacgctt aattgtcgtt gggggtgcct
     4981 cttcgttatt cttagataca gaaaagacga ctcgtatgat tgaccaagta cctaaaaatg
     5041 cacccttcta tccaacagct ttcaatatgt acgaaggatt gttaaaattg aaacaatcta
     5101 aaaatctaca ttggacttat atcagtccag cttctttatt tgatccacat ggcaaacgta
     5161 caggtactta tttgttaagt gatgattatt tacaaagaaa cgctgctggc gacagtacga
     5221 tcagcatggc agattacgcg attgcattag tagatgagat actggatgag aaacatgaaa
     5281 agcagcacat cagtgtagtc agtcgataat acggttattt ttttaatatg ctaaaaaaat
     5341 aaaaatagaa gataaaacca accctgattg atttt
//
LOCUS       DAXWLF010000162         5319 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXWLF010000162 DAXWLF010000000
VERSION     DAXWLF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.165"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            91..371
                     /locus_tag="ACGE4U_002347"
                     /pseudo
     CDS             91..371
                     /locus_tag="ACGE4U_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(449..982)
                     /locus_tag="ACGE4U_002348"
     CDS             complement(449..982)
                     /locus_tag="ACGE4U_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFF8394455.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1076..2254)
                     /gene="mutY"
                     /locus_tag="ACGE4U_002349"
     CDS             complement(1076..2254)
                     /gene="mutY"
                     /locus_tag="ACGE4U_002349"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFF8394456.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2247..3044)
                     /gene="recX"
                     /locus_tag="ACGE4U_002350"
     CDS             complement(2247..3044)
                     /gene="recX"
                     /locus_tag="ACGE4U_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFF8394457.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3151..4533
                     /gene="rlmD"
                     /locus_tag="ACGE4U_002351"
     CDS             3151..4533
                     /gene="rlmD"
                     /locus_tag="ACGE4U_002351"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFF8394458.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4707..5285)
                     /locus_tag="ACGE4U_002352"
     CDS             complement(4707..5285)
                     /locus_tag="ACGE4U_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFF8394459.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         1534 a         1085 c          958 g         1742 t
ORIGIN      
        1 cttttattct ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag
       61 tatactatac gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg
      121 agcaatattt acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt
      181 atgcctttgt ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac
      241 tcagataaaa aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg
      301 aaattacagc ggatggcgat ccttttgaag atgctggcat gcctcatatt tatatgaaaa
      361 agactttata aaaatttatt tcgaaaaaag caaaaaacag caagcttttg agaaaaatct
      421 ctcactggct tactgttctt ttttgtgttt acttacgcga caattgcttg taacggttat
      481 accaaatatc aatatagcct tccgagaacg ggccatctcc attattgatc cagtcaacca
      541 gaatcttcac attttctttt aggatgaagt caatatcgtt cggataattc atcattttgc
      601 tgtgcatctc atattcatcg acatccaata gtcgtttctc gccgtcagga aataccttga
      661 tatccaagtc atagtcgata tatttcaagg cctcatcgtc cagcaaaaaa ggtgaagcta
      721 gattacaata ataggaaacc cccttttctc tgatcattgc tattacattg aaccagtatt
      781 ttttatgaaa ataaacaatc gctggttcgc gcgttaccca tcggcgtcca tcagattcag
      841 tgactaatgt gtgatcattc acgccgatca tagagtgttc gcttgttttt aataccatgg
      901 tatctcgcca cgttcgatgc aatttgccgt cgtgcttgta actttggatc gtcacaaact
      961 ctccttcttt tggaactccc atagcaaccc ttccttaagc agcattttgt gctttgtgtt
     1021 tgggtgttca ttacaccgac atcataatta ttatatcaat aaaaatgagc tttgcctaga
     1081 aaggattcct attgtttcga tttttctcta actgatctac cagtttcatt tggaccttag
     1141 gaaaaacgac cgaatcaaat gcatcaggct tcaaccactt cgaagttttg ttctcggtaa
     1201 attcttgttc tgctccctcg gttgcccgtc cgtaaaaaag gagaacatgc catttcagat
     1261 gactaaaaat atgagtaact tctcctaaat gtcgtgtctg ccaaacaaag agttcctgct
     1321 tttcaaatgg gagattttgc tcttcttctg ctactaggtc atcaaacaga tcgatttctg
     1381 gctttgtttc ccattctttt ttcaatcgct catactcctc ttgtgtcact tccatcattg
     1441 gaaatgtcca catattcgcc agcaattttt ggctatctct ttcttcaaaa taatacgctc
     1501 cagaatgatt ttgaagagcc gcactgatat aataaacgtc ttttggcttt gctttttttg
     1561 tttttacagg aaaactcgtt tggattcctc gtttgttagc taaacaaaag gcttggatcg
     1621 gacaagtctc acactttgga gaagttggtg tacagatagc tgaacctaga tccatcattg
     1681 cttggttgaa ttcacctgga tgcttttcat caatgatttt ccgcatcgct tcatcaaaga
     1741 ttttcctgct agaagctttt gcgatgtctg cttcaatgca aaacaatctg ctgactactc
     1801 gcatcacgtt gccgtcaacc gcgggttctg gaagtccaaa tgcgatactt gcaatcgcgc
     1861 ctgtcgtata tggtcctatt cccttcaatg agctaatttc ttcaggcgtt tgcggcattt
     1921 ccccatcaaa ttctgacatg atctgtttag cagcagcttg gatgttccgg gctcttgagt
     1981 aataaccaag gccctcccat gcttttaaaa gcttttcttc tggagcattt gctagctctt
     2041 caattgtagg aaaccattcc atgaaacgat aaaaataatc gatcactgtg tctactctgg
     2101 tttgctgaag catgatctca gagatccaga tacgataagg atcacgatta tagcgccaag
     2161 gcaggtttct tttttcttgt tcataccatt gaatgaactg atcttgaaat tcttttgttt
     2221 ctttatctgt ccatttttcc cattctttat tcactttcct cttccttttc cgtaatgaat
     2281 cgctggatca tatccaaatc aaatcctttt tgatataagc tttgctttac ttttagattt
     2341 ctttttttag gttcaaacct taaatttctt cgccatagtt tatctccttg tttgactaaa
     2401 taatcatatt ctatttcttg atctgcttct tgcggaagat cagccatcgc ttgttggatc
     2461 acttcttgag aaaaaccttt cgtcatcaat gtttgttgga tttttctcaa taattctttt
     2521 tggctttttc cgcgattttt tccaaacgct ttttcggctg tctgatgtgc attttgaact
     2581 tggtcttcaa atgtatattg gctgagcgct tgttcaatga tttcaggttt caaacctttt
     2641 tgctgcatct gctgcgctaa tttctttggt cctttgtcac ttaagcgcat attcgtgcgc
     2701 acataacttt ccccatagac caaatcatcg acaaggtcca attcttttaa gcgggctacg
     2761 attttatttc gatcttcttg aaggatttcc ttttctttta agtatgttcg gatttctttt
     2821 tccgatcgca actgataact taagtaattc attgctaggt gaaaaccgaa atcagctccg
     2881 ctactcttct tgatctcttg gatagtttct tcgtctaatt cccgtccttt taataatcga
     2941 tagcgtacta atacatcttc agatacttgt aaagcttctc cttcagataa atctacgcga
     3001 tagaaagggc ctttgccttt actgattctc gtaacggtta acataaacat cctcctcgaa
     3061 catatattct ctatcatacc acagaaaatc cccgtaatca ggtattatat gttagaataa
     3121 accacagaaa aaagaaaaag ggagatactt atggcagaaa caattgtcaa aactggacaa
     3181 acggttcctt tgaaaatcaa gcgtttaggc ataaatggtg aaggaatcgg ttatttcaaa
     3241 cggttgatca tatttgttcc atatgcacta ccaaaagaag aagtactagt gaaaatcacg
     3301 aaagcaactc ctcgctatgc agaaggagaa ttaataaaag tcaaaaaaac aagcaaagat
     3361 cgcgtagtgg caccatgtcc tgtttactat gaatgcggtg gctgtcaatt acaacactta
     3421 gcttatcaag ctcaattgga ttttaaaaag gatctgcttt tgcaagcatt ggaaaaattc
     3481 aaaccagcag gctttcgatc ttatcaactt ttgccgacga tcgggatgga tgagccttgg
     3541 cattatagaa acaaagcaca atttcagtta cgaaaaaata agcagactca aaaaattgaa
     3601 gctggtttat acgaagcaaa ctctcatcag ttagtcccta taacagactg cttggttcaa
     3661 gaacctcgaa cacaaaaagt aatgaatact gttgttcaac tattgaataa atttgatgca
     3721 cccatctatg atgaacggac aaacagtgga atcttccgca caatcatggt tagaatcggt
     3781 gttaaaactg gggagttaca agttgttttt attactcgca gtaaaaaatt cccgcaaaaa
     3841 aacgcaatga tctgtgagat caatgaacga ctgcccgaag ttgtttcaat catgcaaaat
     3901 gtccaggcac aaaaaacgtc tctcgtgatg ggggatgaaa cgatccactt atggggaaaa
     3961 gaagccattg aagaacaaat caatgaagtg actttcgatc tttctcctcg tgccttcttt
     4021 caactcaatc ctcagcagac cgaattgctt tatggcgaag gaatcaaagc attagatgtc
     4081 cagcctaatg aaacgatcgt ggatgcttat tgcggagttg ggacgatcgg cttaagtgta
     4141 gcaaaacaag ccaaagaagt acggggaatg gataccgttc ctcaagcaat cgctgatgca
     4201 aaaaaaaatg cggaaagatt aggatatcac aacactcgat atgaagtcgg gacagcagag
     4261 caagttctgc ctaaatggtt gaatgaaggt tttcagcctg atggtatcat cgtagatcca
     4321 ccacgtacgg gtttggataa cgcattgatc caatcattat tgaaataccc tccaaaaaaa
     4381 cttgtttata tctcctgtaa tgtttctaca cttgccagag atctggttaa tttaagtaaa
     4441 aagttcaaga ttgaatattt acagtctgtc gacatgtttc ctcagactgc acgttgtgaa
     4501 gttgtcgtga agatgacgag actcgctgac taaatgaaag aggttgtgaa aaagtctgcc
     4561 ctgtatcaag taataagaac agccaaacaa aaatagctct tcatgtttta tgaataccgt
     4621 acttgtgacc gaagagttaa caggaggaca ctatttattt aaaaaaaggg ttgtgacata
     4681 gtttttgtca cagccctttt tgattattat tggatattca cgtctatcac ttgataaaat
     4741 gcatttgtcg tatcgctgat attccatact gctaaaatca cattatatcc ttttcgatcc
     4801 tgcggaagtg tgatcgtttg gttgactgtt tttggtggaa cagttgcttt gtcgtctatt
     4861 tttgtgatca attcaaaatc actaaatttc aaaggttgat taggattcca agttggtttg
     4921 gtaatgtagt aatcccaagt acttgtccga tgttgtgctg ttaaattcca agtaatattt
     4981 aatgttcctg gattaaccat gtttttatgc catctgttag atgtctgttc atctaaaggc
     5041 tcaaagccgc tgactcctgc actagcaatt tttccatcaa tgaatgtatt tttggtagct
     5101 tcgatacttt gcggttccca ttgtgctctt ccgacatttt gatttaagtt tcctgctgct
     5161 gatgttccta ggtatgcacg gcttcctgga gaagtaatat aaccatgcgc aaaacctgga
     5221 atgctaaaac cgaacaaccc taaactaact aacccaataa acaataacgt actaatcttt
     5281 ttcatgtaga aacctccttc atttgataac cacattgta
//
LOCUS       DAXWLF010000163         5307 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXWLF010000163 DAXWLF010000000
VERSION     DAXWLF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.166"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(57..1196)
                     /locus_tag="ACGE4U_002353"
     CDS             complement(57..1196)
                     /locus_tag="ACGE4U_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8394460.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(1505..2218)
                     /locus_tag="ACGE4U_002354"
     CDS             complement(1505..2218)
                     /locus_tag="ACGE4U_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFF8394461.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(2472..3173)
                     /gene="nagB"
                     /locus_tag="ACGE4U_002355"
     CDS             complement(2472..3173)
                     /gene="nagB"
                     /locus_tag="ACGE4U_002355"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFF8394462.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            3407..4939
                     /locus_tag="ACGE4U_002356"
                     /pseudo
     CDS             3407..4939
                     /locus_tag="ACGE4U_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
     gene            complement(5130..>5307)
                     /locus_tag="ACGE4U_002357"
     CDS             complement(5130..>5307)
                     /locus_tag="ACGE4U_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8394463.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         1745 a         1079 c          892 g         1591 t
ORIGIN      
        1 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
       61 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
      121 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
      181 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
      241 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
      301 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
      361 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
      421 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
      481 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
      541 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
      601 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
      661 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
      721 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
      781 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
      841 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
      901 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
      961 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
     1021 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
     1081 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
     1141 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
     1201 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
     1261 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
     1321 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
     1381 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
     1441 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
     1501 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
     1561 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
     1621 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
     1681 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
     1741 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
     1801 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
     1861 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
     1921 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
     1981 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
     2041 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
     2101 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
     2161 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
     2221 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
     2281 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
     2341 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
     2401 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
     2461 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
     2521 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
     2581 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
     2641 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
     2701 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
     2761 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
     2821 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
     2881 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
     2941 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
     3001 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
     3061 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
     3121 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
     3181 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
     3241 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
     3301 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
     3361 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
     3421 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
     3481 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
     3541 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
     3601 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
     3661 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
     3721 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
     3781 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
     3841 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
     3901 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
     3961 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
     4021 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
     4081 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
     4141 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
     4201 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
     4261 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
     4321 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
     4381 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
     4441 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
     4501 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
     4561 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
     4621 aagcagttaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
     4681 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
     4741 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
     4801 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
     4861 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
     4921 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
     4981 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
     5041 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
     5101 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
     5161 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
     5221 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
     5281 ccaataaagt acataaacat aaataca
//
LOCUS       DAXWLF010000164         5225 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXWLF010000164 DAXWLF010000000
VERSION     DAXWLF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.167"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            142..459
                     /locus_tag="ACGE4U_002358"
     CDS             142..459
                     /locus_tag="ACGE4U_002358"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8394464.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            489..809
                     /locus_tag="ACGE4U_002359"
     CDS             489..809
                     /locus_tag="ACGE4U_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8394465.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            917..2377
                     /locus_tag="ACGE4U_002360"
     CDS             917..2377
                     /locus_tag="ACGE4U_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8394466.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            2445..2666
                     /locus_tag="ACGE4U_002361"
     CDS             2445..2666
                     /locus_tag="ACGE4U_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394467.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            2697..2879
                     /locus_tag="ACGE4U_002362"
     CDS             2697..2879
                     /locus_tag="ACGE4U_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFF8394468.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            2879..3292
                     /locus_tag="ACGE4U_002363"
     CDS             2879..3292
                     /locus_tag="ACGE4U_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFF8394469.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            3415..4596
                     /locus_tag="ACGE4U_002364"
     CDS             3415..4596
                     /locus_tag="ACGE4U_002364"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8394470.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(4667..4840)
                     /locus_tag="ACGE4U_002365"
     CDS             complement(4667..4840)
                     /locus_tag="ACGE4U_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394471.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTPKTKE
                     NKKFKFTKKEDLL"
     gene            4994..5067
                     /locus_tag="ACGE4U_002366"
     tRNA            4994..5067
                     /locus_tag="ACGE4U_002366"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5028..5030,aa:Arg,seq:cct)
     gene            complement(5136..>5225)
                     /locus_tag="ACGE4U_002367"
     CDS             complement(5136..>5225)
                     /locus_tag="ACGE4U_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFF8394472.1"
                     /translation="TYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT         1673 a          875 c         1101 g         1576 t
ORIGIN      
        1 catactgttt acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga
       61 aaacgctatt tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa
      121 aaaataaaag gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg
      181 atgagtacga gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa
      241 gccgatattt ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc
      301 gatgtattat tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt
      361 gcgcctaaag gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga
      421 gaaaaagttc ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt
      481 ataacaacgt ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg
      541 gtaatgcaaa gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt
      601 tagcggatag gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa
      661 cagacatgtt gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc
      721 acagtcaaga tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg
      781 aactttatcg tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa
      841 aatttttgaa cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta
      901 taggaggaag taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt
      961 tgccgcaaaa atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcac
     1021 actgcccgca acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt
     1081 accgcaacag tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc
     1141 cgtattgaat gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc
     1201 cggattgatc tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga
     1261 tctagctggg gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc
     1321 attatcagca gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc
     1381 tgagattggt gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa
     1441 actagatcat ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt
     1501 cattattttt tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc
     1561 accagcagta tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat
     1621 tgctattatc aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt
     1681 gcaaaaatat attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat
     1741 cgtcacagta tttgttcaag tattctggtt ctttggtatt catggaccaa atgtattggc
     1801 accagtgcta gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa
     1861 aggttataac ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc
     1921 ttatatgtgg gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac
     1981 gatcgtctta ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc
     2041 taaattatcc attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc
     2101 aatcgtattg aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc
     2161 aattggatgg acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg
     2221 ggtagtaccg ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat
     2281 cgtaacatta gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc
     2341 aaacaaaatg gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc
     2401 tttaagtttc gttcctcata atgaattttc gaggaggaac tgaaatgatt acaattgcca
     2461 gtgcaatgca acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga
     2521 cttttacatt taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc
     2581 cggaagttgc ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc
     2641 tttattttca atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga
     2701 taaaaaacgg tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc
     2761 cacattcgca aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag
     2821 gaatctattt cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat
     2881 ggaaatcacg aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc
     2941 tgtccttttc gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa
     3001 tttggagtac gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt
     3061 cattgaaaac cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca
     3121 ttatcaaatc aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga
     3181 gtcttatggc atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt
     3241 taaacggaga cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga
     3301 aacatggtac tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa
     3361 gatagatctt tcctgcgaat cctcactagc ttttttgtag gaaggggcag caaaatgaca
     3421 aacaaagtaa gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat
     3481 gcatttaatg gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat
     3541 tcttggaatt acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc
     3601 ccttttccag tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct
     3661 tcttatccag aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa
     3721 gattgccgtg atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat
     3781 cggcgctatg gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat
     3841 gtgccgttta tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa
     3901 gctgcaatca gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc
     3961 gaagactggt ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat
     4021 tttgatgata atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt
     4081 attattgcgg agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta
     4141 agttctcaca atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat
     4201 caaaattatc tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg
     4261 aggatcgtag atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca
     4321 tttgccctac aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta
     4381 acaatcgaaa atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt
     4441 cgaggaagta tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg
     4501 gtattccaag aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg
     4561 ccacagcaac agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt
     4621 tttgtcacca cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct
     4681 cttttttcgt aaatttgaat tttttgtttt cttttgtttt aggtgtttta gggttttctt
     4741 ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta gatgaatcta
     4801 agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac ctccctacat
     4861 tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt tttgcctaac
     4921 ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa gctacaattg
     4981 atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct aagcgatagg
     5041 tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt gcggtacagg
     5101 ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt atataaacta
     5161 tcgaacacag tattgatttt ttctttatcc tgatcttcaa gctctcgaac aatatgaaga
     5221 taagt
//
LOCUS       DAXWLF010000165         5192 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXWLF010000165 DAXWLF010000000
VERSION     DAXWLF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.168"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            34..2259
                     /locus_tag="ACGE4U_002368"
     CDS             34..2259
                     /locus_tag="ACGE4U_002368"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFF8394473.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            2277..4160
                     /locus_tag="ACGE4U_002369"
     CDS             2277..4160
                     /locus_tag="ACGE4U_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFF8394474.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            4141..5115
                     /locus_tag="ACGE4U_002370"
     CDS             4141..5115
                     /locus_tag="ACGE4U_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFF8394475.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         1701 a          927 c         1153 g         1411 t
ORIGIN      
        1 gtaaacggtt tccgttttgt tgggagtgaa atgatggata ttacgcatag aaaaacagat
       61 aaaaatttca tcttatttga tgaaaagagc cgagtcttcc atttacgaaa tacatttctt
      121 tcttatctga taaagataga agaaagcaat gtattggcgc atatttattt tggcaaacca
      181 gtcaagcagt acaaagacaa taaaaattat ccccgaagag atcgtggatt ttcaggaaat
      241 gttcccttaa atcctgaccg gtctctgtcg aaagatacac taccgcaaga atactcttct
      301 catggcagca tggactttcg aacgcccgca tcgatcattc agcgcaagaa tggatcagac
      361 ttactggatc tgagatatga cagccattat attacagatg ggaagccaga gatcgaaggc
      421 ttgcctcaaa cttatgtgat ggacaagtca gaggcacaaa cgttggtgat ttcacttaag
      481 gatagagaaa cagcgattta tttcgatttg ttctatacca tatttactga tagagcagtg
      541 atcacacgat ctgtaaagat aagaaatgaa accggagaaa caatcaaact agaaaaagcg
      601 gctagtttcc agctagactt tgcccatact aggcgatttg atgaagtcat tgctttgcca
      661 ggagcacatg tcaatgaaag acagatttca cggcaatctg ttttatctgg aacaaaagta
      721 tttgaaagcc gtcgaggaac gagcagtcac catatgaata actttattgc tttagttcat
      781 catcatacga cagaaaatac tggagaagcg atcggacttc aatttgttta ttccggcaac
      841 cattctttcg aacttgaaaa agatcagatc aatcaattga gagtcgtcgg tgggatcaac
      901 agccatcgtt tttcgtggga attgaatgca ggccagtctt ttcaaacacc tgaaatgatc
      961 ctttcttact caagtcaggg attgaacaaa atgagtcaga tccaccatga gttgctacgt
     1021 gaaagaatcg caagaggtcg tcatcaattt gctgaacggc cgattctggt caataactgg
     1081 gaagccactt actttgactt caatagtgaa aaaatcaagg cgatcattga tgaagcaaag
     1141 gagctaggca tcgaaatgtt cgtattagat gatggctggt ttggaaaaag agatgcggac
     1201 aattcttcat taggagactg gttcgagtat gaaggcaagt tgacaaatgg attacgtgaa
     1261 atagcagatt atgctcatag caaaggattg aaatttggtt tatggtttga acctgaaatg
     1321 atctcggttg attctgaact gtatcgaaca catcctgatt ttcttatgca ggagccaggt
     1381 agaatgcctt ctgcttccag atcacaacat gtactggatt ttacaaggtt agatgttcgg
     1441 cagacgattg agaaacaaat gagaaagatt ttagatacga ttcctcttga ctacatcaaa
     1501 tgggatatga accgttcgct ttcagatgtc tattcgatta cccttgaccc acagagacaa
     1561 ggagaagtag cgcatcgtta tatgctcggg ctttatgaac tacttgaaca tttgacaact
     1621 gattatccgg aaatcttatg ggaaggctgt tctggtggag gtggacggtt tgatgccggg
     1681 tttatttatt atatgccgca gtcatggaca agtgataaca cggatgcagt ggagcgtatg
     1741 aaaatccaat atggaacaag ccttgcttat ccgatttcgt ccatcacagc tcatgtatca
     1801 gctgtaccaa atcatcagac cgacagatct acttcgttga aaacaagagg agaaacagct
     1861 atgagcgctg tttttggtta tgaactggat ttgacaaagc tgtctcctga agaaaaaaag
     1921 caagtaaaag accaaattat atcctatcaa acgattcgtc cggtgatcca gtatggccat
     1981 tattatcgtt tggcgagtcc ttttgaagaa aatattgccg catggatgtt tgtgtctcct
     2041 aaacaagatg aagctattgt gtttttaggg aggatactag cttcagctca accagcgttc
     2101 catgaagtat atctgatggg gttagatgat gaggcacttt atcaggaaca gacctcgaag
     2161 cggatatttt cgggggccga attgatgaca gttggacttt acttccccga ttttcaaggt
     2221 gatttccaaa cagaactgct tcatttcaaa aagttatgag agagaaggaa aaaagtatga
     2281 aagcaaatat aaaatggtta gatgatccac aagtttttag agtgaaccaa tcaaatgcac
     2341 atagcgatca tgtttgttat gcaacaaggg aagaagtaga aaccacatcc tcccttaaac
     2401 agtctttaaa tggatcatgg cgtttttgtt attcgccaaa tccttcggta cgaccaaaaa
     2461 cattttataa aaaagatttt gatgcctgtt cttttgatga aatcactgtt cctagtcata
     2521 ttgaattagc aaattatgac aagatccatt acatcaatac gatgtatccg tgggagggac
     2581 atgtatttcg ccgtccccct ttcacgacaa gccctgagaa agaggcggaa gggatctttt
     2641 cagctgctaa agacaatccg gttggttcgt atttgacgac ttttcgatta gaagaaggtc
     2701 taaagggaaa acaaatctcg attcattttg ctggtgtaga acaagcaatg tttatttggt
     2761 tgaatggtca ttttatcggg tatgccgaag attcgttcac tccgtctgaa tttgatttaa
     2821 caccttattt ggaagaagaa aatattctag ctgtggaggt ttacaaaaga agtacagctg
     2881 cattcttgga agatcaggac tttttccgat tttttgggat cttcagagat gtcttcttag
     2941 ttgctcgagg acaaaaccat ttagaagatc tttggatcaa acctcgctta gatgacaaat
     3001 gtcaaaatgg caaacttgaa gtagatttgt cttttgagca agtagccaaa aacagtcagc
     3061 tgaatgtagc gatactagat cctgatggga aggaaatcta tcaacagcaa tatccgttaa
     3121 aagagcaaat gacgattcgc atcgatggat ttgaaaatgt ggcactgtgg gggcattttc
     3181 agcccaattt atataagctt gaattggaga cgatagacga agaaaaaaga gtaacggagt
     3241 atattgacta ttccttcggt tttcgtaaaa tcgaaatcaa agataaaatc atctacttca
     3301 atggaaaacg gatgattatc aacggggtaa atcgacatga gtggcatccg aaaaaaggac
     3361 gcgccatcac tatagaagat atggaaaaag atttggctat cataaaaaat gcaaagatca
     3421 acggcgtacg gacatctcat taccccaacc agatcacttg gtattattta tgcgaccaag
     3481 caggaatcca tgtgatggcg gaaacgaacc tcgaatccca tggctcatgg caaaaaatgg
     3541 gcgcatttga gccttcttac aacgtaccag gtgcagtacc tcagtggcag gcggctgttc
     3601 ttgatcgagc aaaaacgaat ttcgaaacat taaaaaacca tccaagtatt cttttttggt
     3661 ctcttggaaa tgaatcttat gcggaagaaa acattcgaca aatgaacgat tacttcaaaa
     3721 aagtagaccc cgatcgactt gtccattatg aagggaattt tccaaatcca gcattcgaag
     3781 attcgatttc agatgtaaaa agctgcatgt atgcgccacc agaagagatc attgactacc
     3841 ttgaaaataa agcggaaaaa ccgttcatct tatgtgaata tatgcatgac atggggaatt
     3901 cgttaggtgg aatggattct tacatgacgt tgcttgatcg ttatgaaatg taccaaggag
     3961 gctatatatg ggattttaaa gatcaggcga tccaagtgac tgatccaatc acaggcagag
     4021 aagtgatgcg ctatggcggt gactttgacg ataaaccaag tgactatgaa ttctcaggga
     4081 atgggatcgt ttttgcagat ggacaagaaa aacccgccat gcaggaggta agatattatt
     4141 atgaaaaata cagtaaataa aagtcatatg gatacggaaa aagttgcaat cgtcttcggc
     4201 gactgtacat taggtgtcaa atcggggaat acgcattata ttttttctta tacaagaggc
     4261 ggactggaat cgctcaataa aaacggaaaa gaatggctat accgggaaac acttccagtt
     4321 ttttggcgag caacgaccga taatgacaga ggtagcggat ttggatttaa atccggtatg
     4381 tggttaggag ccggattgta tccaaaagtc aaagaaatag aagtactggt ggacaataaa
     4441 aagatcgatt tgccgattgc tccagtaaat aatcaatatt caagtaatga atacgctgaa
     4501 acggttgaaa tcatttttac actggaaatc aacacagtac caaaaaccca agtgacaatt
     4561 tcctaccaaa tcgctggaga gggtaaaatg cttgtcaata tgcattatta tggacaaaaa
     4621 ggattaccgg aattacccgc attgggttta aggttcatta tgccaacgaa agcaaaattt
     4681 tttgagtatg aaggcctatc aggggaaaca tatccggatc ggatggctgg tggtgtcgaa
     4741 ggtatttata aagtggcagg actccccgtt tccccatacc ttgttcccca agaatgcggc
     4801 atgcatatgg aaacgaaatg ggtagaacta acaagaaatc aaacgttgga taatacggat
     4861 aaagatcaag aagatttttc gttgcgattt gaacagcagg atacgccatt tgctttttcc
     4921 gctttaccat tcaaagcaga agaactggaa caggcaacac atatagaaga actaccgcca
     4981 gaaagacgaa cggttcttac gatttttgca aaagtaagag gagtcggggg tatcaatagc
     5041 tggggagcgg atgttgagtc accttaccga atcagcagcg aagaggacca tgaaatcggc
     5101 tttattattc agtagcaatc tttagattat tatttgaaaa acggagttta caagacaaag
     5161 ctgctttatt ctatatgtca tgaacaagag ag
//
LOCUS       DAXWLF010000166         5128 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXWLF010000166 DAXWLF010000000
VERSION     DAXWLF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.169"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            53..1987
                     /locus_tag="ACGE4U_002371"
     CDS             53..1987
                     /locus_tag="ACGE4U_002371"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8394476.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2444..2860
                     /locus_tag="ACGE4U_002372"
     CDS             2444..2860
                     /locus_tag="ACGE4U_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFF8394477.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2874..3440
                     /locus_tag="ACGE4U_002373"
     CDS             2874..3440
                     /locus_tag="ACGE4U_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFF8394478.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3463..4449
                     /locus_tag="ACGE4U_002374"
     CDS             3463..4449
                     /locus_tag="ACGE4U_002374"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFF8394479.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4604..>5128)
                     /gene="scm"
                     /locus_tag="ACGE4U_002375"
     CDS             complement(4604..>5128)
                     /gene="scm"
                     /locus_tag="ACGE4U_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFF8394480.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         1545 a          948 c         1134 g         1501 t
ORIGIN      
        1 aagaattatc atatattcat tatatagata gtctataagg agcggatgat caatggcaaa
       61 gcaaaatatt gtcgttgtag gagcaggata tgccggtgtt tcagctacaa aatacttggc
      121 aaagaaactg aaaaaagaag atgtagcaat tactctgatc gatcgtcatt cgtaccatac
      181 tatgatgacc gagttgcatg aagtagctgg cggacgtgta gaaccagaag caatccaata
      241 cgatctgcag catttgtttg ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat
      301 cggtatcgat aaagaacgaa aaatcgtcaa gaccaaatta ggttcctatc cttttgatca
      361 gctgatcctt ggaatgggcg gagagccgaa tgactttggt acacctggtg tcaaagcaca
      421 cggattcacc ctttggtcat ttgaagattc tgtaaggatt cgggaacata ttgagaagac
      481 agtagcaaaa gctgcattgg aaccagatga tgaactgcgt aaagccatgc tgactttcgt
      541 tgtctgtggt tctggattta ccggtattga aatgattggg gaactgatcg actggaaaaa
      601 tcgcctagcc aaagattata aattagatcc aaacgaaatc acactgatgg tcgtagaggc
      661 aatgccgacg atcttgaata tgttggatcg aaatgatgct gcaaaagctg aacgctattt
      721 gcgtaagcac aacgtggaat tattattgaa tgcaccaatc gtagaagtgg ctgctgatca
      781 tatcaaattg aaagacggac gcaacattcc gactcacaca ctgatttgga cagctggtgt
      841 caaaggaact tctgacgctg ctgactttgg attagaatca gcacgcggac agcggttgat
      901 cgccaatgaa tatatgcagg caaaaggcta cgaagataaa aacatctata tcattggtga
      961 tttagtcttt tatgaagaat tcccaggaac accgacaccg cagatcgttc aagctgctga
     1021 acaaacagga cacacagccg cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca
     1081 taaatttaaa ggaaactatc aaggatttat ggtttctatt ggtgccaaat ggggggtagc
     1141 taacctattt gataagatcc atttaagcgg atttttagct atcgtaatga aacatatcgt
     1201 gaatttgaaa tacttctttg atattcgttc tggttattat atgttccaat atttaatgca
     1261 tgagtttttc catatcaaag atgatcgcaa cgtgactcga ggacattctt ctcgttacgg
     1321 caatgtctta tggagtgtgc cacttcgtat tttctatggt ttagtttggt tcgttgaagc
     1381 ttccaaaaaa atcattggga atggtgaagt gttcaaacca agtacttggt ttggtgaagg
     1441 atcatggttc actggaaaca gtgtgttccc ttggacatgg gaatacaatg taacagatgt
     1501 tgccagtggt gcttctgctg ctggtgatgc aaccacagct gccagcggag caggggcaag
     1561 tgccggagaa gcagcgactc aagctgcaca ttttggtttg agttatgcat acggtgaaga
     1621 accaatggct gtgattgaga agtcaccaga ctggttcaca aagatcatgc aagtgatcat
     1681 gccaaacaat gatgtagcaa tgttcttcca aaaattcatg gtattctttg aaattgcctt
     1741 ggcattagca ttgattgctg gattattcac atggctatgc agtgcgacaa cgattgtatt
     1801 ggtgatcacg ttctgcttat caggcatgtt ttactgggta aacatttggt tcatttttgt
     1861 tgctttcgca ttgatgaacg gttccggacg tgcgattggg ttggatcgct gggtaattcc
     1921 atggctacaa cgtacactag gtaattggtg gtacggtaga ccaagttccc agtacggcaa
     1981 gaaataaata gataaagaac gttatttgaa tgcaagattt caatagaaag gggctgagac
     2041 aaaccgtcac agcctccttt ttatgtaaaa ttaaaggaat aagatgcccg agataagcct
     2101 ccatgagcaa gacacaggtg tcacaacctc tgttacaacc tcttgttata aacggtgttc
     2161 cagaagtagc ttcttcgaaa aataagccga aatttcacaa aaacttgaaa agtaattttc
     2221 gtaaattcct tcttatttct tgaagttgcc cacttctgtc acaacctctt gttataaacg
     2281 gtgttccacc tgctgcttct cggtaataag cccaaagaat tagaaaacat ggaaagctgt
     2341 tttctattct tcgttcttat tactcaaagc aagacacagg tgtcacaacc tcttgttttt
     2401 atgctaaaat tgggtgagtt aatctggaaa aaggagaaca aaaatgagcc ttaaagaatt
     2461 tgctgaaaaa agtctgctca aaccttggga tgtttttatt attttgcttt tgactgttct
     2521 ttctttttta ccagtcatga tttttggtct tcagcaaaac accgcaacag cagaaaaaga
     2581 agcggtactg cgtgtggatg gcacagaaat caaaacattc cctttgatgg ctggagaaga
     2641 gagcaacaca tatacttaca aagatgaaca cggagattat aacttgatcg agatagatgg
     2701 cgatcgagta cggataaaag aagcagattg cgatgaccag atctgcgtac gtcgaggctg
     2761 ggcaacgaaa aatggcgaaa ccatcgtctg tttgccacat aaattagtga tcgaagtccg
     2821 ttcaacagat ggaggtgacg aagacagtct gatttattag accttcacac accatgagta
     2881 aattacaaaa aatgatttat atttcgctac ttgttgcaca aggagtgatc attggtttat
     2941 tggaaaatat gatcccttat cctttttctt ttgcgcctgg tgcaaaacta ggcttagcca
     3001 atctaatcac gatcgttgcc atttttacca tgaaaaaaca agacagcttc ttactacttt
     3061 ggctacgact attcctgact actttgctag gcggaacgat ttccacattc ctttatagta
     3121 tgagtggtgc tttgcttagt tattttggaa tgttattggt caagcaatta ggaccaaaac
     3181 gtgtcagtat catcggaatt agtgcggccg gcggtttcat gcataatgtc ggacagcttt
     3241 tgatggcttc tttcattgca caatcatgga cagtcatgtt gtacttgcca gtcctttcag
     3301 ttttagggat tttatcaggg attgcaatcg ggatcgcagc gaattatctt ttacgacatg
     3361 tgcagacact tcaacgattc caaaaagatt atgagcaggg aaccaaacaa aaatggagcc
     3421 caatgtttac caaaaaataa tcgtcacaca aggagttttt ttatgaagct acatccaatg
     3481 tgggaaaact atcctgcttt aagtaaagaa ttgacagaaa cactaaaacg gatggaagaa
     3541 actgtccgcc tgaaaaacaa acctgtcgaa caagccatca aagaaacgat ccatgccggc
     3601 ggaaagctgt tgcgtcctgc ctatcagcta ttgttctcac aatttggacc agagcaagat
     3661 cgagacaaag ccatcgcttt ggcagcttct atcgaaatgc tccatacggc cacactgatc
     3721 catgatgata tcgttgatga agcggatctg cgacgtggtc agcctaatat ccgtagtcag
     3781 tttggtaatg tcgtttctgt ctatgcagga gactatttat ttgtctgctg ttttaaacta
     3841 ttgtctgatt actccacttc cttgaaaagt ctccaattga attctcgcag tatggaaaaa
     3901 gtattgaatg gcgaattagg gcagatggat gatcgctata attatgaact ttctgtaaaa
     3961 gaatatttgg aaaatatttc aggtaagaca gctgaactgt tccaattaag ttgttctgtt
     4021 ggcgcttttg aaagtggaac aagcgagcgc ttcgcaaaaa aagcaggaga tatcggactt
     4081 tctatcggga tggcctttca gattatcgat gatattttag attatacaaa agagagccag
     4141 gaaatcggca aacctgtact agaagacatg cgtcaagggg tctattcttt accgttgatc
     4201 tactctttgc aaaaaaacaa accaaagcta ctaccttatt tagagaagaa agcggcaatg
     4261 acagaagaag atgtcgacgc cgtacgcaaa atcgttgaac atacaggagg cgtggaagaa
     4321 gctaggaagt tagcggcctc ttacacagaa aaggctttga aggaaataaa gaaattacct
     4381 gccacctctc ttcgaacaaa agagaatctc ttttccctta cccaactgat tttggatcgc
     4441 aaagactgaa tagggaaagg ttcgatacgt aaataaattt ctcagaagga aatctacgtg
     4501 ttttttgaca aattgaattg tagttcttgt atttatgata aaaagcaaaa ataaagagca
     4561 acgtccgtta gcgacgttgc tcttttttat cttgaatatt tttttacctt ttcgatttct
     4621 tataactcat aaaagtgatt cttaaaccga ttacaagtaa tataacgcct gcgagtgtta
     4681 tccaattatt cgattgactt ccagttttag gaagtgctct ttttgatttg tttccattgt
     4741 cattgcttgt tttattagtt aagttaaagt tcgtcctgtt atccgaactt gtttttgaga
     4801 aggagacact tgcatgacgt gtatcgcttg tggtagacga cgtgtctgta ctatcgcttg
     4861 aagttgatga atctgagcta tcacttgtgg tagaggatgt atctgtactg tcacttgaag
     4921 ctgacgtgtc cgtgctatca cttgtagtgg tagatgaatc tgagctatca cttgtggtag
     4981 aggtagtatc tgtactgtca cttgaagctg acgtgtccgt gctatcactt gttgtggtag
     5041 atgtatccgt actgtcgctt gaagctgacg tgtccgtgct atcacttgtg gtagaggtag
     5101 tatctgtact gtcacttgaa gctgatgt
//
LOCUS       DAXWLF010000167         5046 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXWLF010000167 DAXWLF010000000
VERSION     DAXWLF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.170"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..919)
                     /locus_tag="ACGE4U_002376"
     CDS             complement(<1..919)
                     /locus_tag="ACGE4U_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016388609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFF8394481.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFDRASRFAEEMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDV"
     gene            complement(932..1807)
                     /locus_tag="ACGE4U_002377"
     CDS             complement(932..1807)
                     /locus_tag="ACGE4U_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFF8394482.1"
                     /translation="MRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPFT
                     SFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAINV
                     GIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKVFVGIGLVIAALLIFAGQMNWIPSNG
                     TASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACTF
                     SVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYVLKWIVILVLLYSA
                     YTMLGSQLKKVSAAN"
     gene            complement(1826..2626)
                     /locus_tag="ACGE4U_002378"
     CDS             complement(1826..2626)
                     /locus_tag="ACGE4U_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFF8394483.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENLTDSLLKTLQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESSEELKAKYGEDFEGIRKIY"
     gene            complement(2901..3050)
                     /locus_tag="ACGE4U_002379"
     CDS             complement(2901..3050)
                     /locus_tag="ACGE4U_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394484.1"
                     /translation="MKKKVIPYCPKPKNAFERGLFKYFEGKKGQDHKEENQKKKSKKK
                     SEEFF"
     gene            3551..3892
                     /locus_tag="ACGE4U_002380"
     CDS             3551..3892
                     /locus_tag="ACGE4U_002380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394485.1"
                     /translation="MKANTPYKVGDIFYSSWGYEQTNVNFWQIIKLTEKTAWFRPIKK
                     QTVKTYTSMSGETMPIPNEFIDYPLVRTKDSIVRKRINPALYPNELYGNRSWDTFYRY
                     DNQPVYESSYY"
     gene            3915..4058
                     /locus_tag="ACGE4U_002381"
     CDS             3915..4058
                     /locus_tag="ACGE4U_002381"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394486.1"
                     /translation="MLKITKIKGARTMKPITLYEILGEERSDNLPTTHKLRVLASKSI
                     NKK"
     gene            4092..4385
                     /locus_tag="ACGE4U_002382"
     CDS             4092..4385
                     /locus_tag="ACGE4U_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394487.1"
                     /translation="MLRTNAIPIVDSYDRNTNNYLGSFEQTDENILNYVAGLSPFQSV
                     RLVEHTTDTLILTTIGYFFDQVSDQQWLQQILPKLIAKQTGKKTIEAVKIFFY"
     gene            4440..4988
                     /locus_tag="ACGE4U_002383"
     CDS             4440..4988
                     /locus_tag="ACGE4U_002383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394488.1"
                     /translation="MAILQPFGEPIERTEFIQHYMKLFVQVIKHTHQIDEFYSKEIEY
                     LLAEKQKIALLYDYFVEMYDRAPDYFYLSDTLTTNFLAKEYLFASHTKNFMCVEHFVN
                     TYLHLLKTQKICTFEAFQTDYLFILDREAYHAKQAFEKQNQAIEGYPELRIQNNSFLQ
                     QRLLKQLINGFHQRNKGYQKDQ"
BASE COUNT         1631 a          985 c          789 g         1641 t
ORIGIN      
        1 ccacatctaa tacagccgaa ccaactgttt tgaagattgt gatttgttct ccacttgtcc
       61 gaccgatcaa ttcttcattg atcagttggc ctagttcccc atgataactg tctctttgaa
      121 tatacccatc ttgcagcggt gaaatgaagt ccccagcttc ttttagaaca ccgtccatgg
      181 tatcaaaaat aacaacatct gcggctttga ttatttctcg tggaatttca cacatttccg
      241 gtgtgtaagc accgactccg ttgatgtgcg cacctttttt gacccactca gcagagaatg
      301 tcgcacgttt ggaagtggtc acacttgtaa tgatatctgc tccttcaacg cattcttgac
      361 tggtttttgt tgggcgcatc gttacagaaa actgttgcat catttcttca gcaaatcggc
      421 tggcacggtc aaaatcaata tcaaaaatcc gaacttcttc taatttacga acggtcaaca
      481 tcgcttctaa ttgcgactgt gcttgtccgc cggttccgat cagtgcgccg attttagcat
      541 cttctcttgc aagaagttct gttgctgcac cttgaacagc gccggttctt aattgtgtca
      601 aataggtacc atccaaacaa gcggatacca tacccgtttc tggatcaagg acgatcatcg
      661 ttgctggtac acttggtaag tttttcttga tgttttcagg atacacggaa acgattttga
      721 cacctaacgc accttcgcca ccttctacat aagctggcat atataagctt tgtccgttgc
      781 tttttggcac atcgatattg gtgcgaagag gcacacttgc ctttcctgct gaatacaatg
      841 acaatgcttt tttgtctgca tcgatggctt ctctcattga gaaacttttg ataatatctt
      901 cttttttaag aaataacatt tgaatcgctc cttaattagc tgctgaaact ttttttaatt
      961 gactgcccaa cattgtatat gcactgtata gtaagaccaa aattacgatc catttcaaga
     1021 cataggtatc taaagatttg actaggaata cagcgaccaa tacccccaat acaccgaagg
     1081 ttgaagtgaa aagtgtgatt tttctgctgt attcattaaa tttaatgaat tgaacactac
     1141 cgatcggtac tgagaatgta caagcaccca tcatgatagg aaaggctgcc actggattca
     1201 agcctagcaa gtagacagtt accatcgtta acgcataaga accaatgccg atattgttca
     1261 atgcgccata gacaaagagt aaaaatcctg ccaagattaa ttttccgcca tacaattcac
     1321 tggctgttcc gtttgatgga atccagttca tttgtcctgc aaaaatcaat aatgcagcaa
     1381 tgaccaatcc tatgccgaca aacactttga tcgttttctc tggtaacttc acgacaaatc
     1441 ttggtcctaa atatgcaccg ataatttgac agataataca aacagctagt gtcttgatcc
     1501 ctacgttgat ggcagtgata taagacaatg ccatgacggc taccggtacg acacattggg
     1561 tattcaacgt tccgggtagt tttttcatgc tgacccattt caactttgga taaagaacgg
     1621 ttccgatagc aaagtcggaa acgccaaatg ttgaaagaaa gaagatgata aatgacgtga
     1681 agggcaacag acgcatatcc ccaggttctt ctttgaaatt ttgtttgttt ttgaaaaggt
     1741 ctcttagaaa gtaaataata aaatatagat tgattgcgac aattagtgtc agcaaaatat
     1801 tccgaatcct aaaaatttcc tcctactagt aaatctttct gattccctca aagtcctcac
     1861 catattttgc tttaagttct tcagaagatt ctgcaatcac ttttagtgta tatgcatcat
     1921 gtttgacata ttcttttgct agattgacca tggcgtggtt ttttgtccaa gtgacatcgg
     1981 ttgattgcag ttcttcgtta atggtgccaa acacgacttc ttgctcatcg gcgacgatgt
     2041 ttatccatct tgctccaaag tcagcaagtt tgtctgtttc aaagccatga atataatatt
     2101 tttctaaggg aagatcatat tcatgggtag aactgaataa aattagtaca aacttatcaa
     2161 ctcttttttc agcttgctgc aatgtcttta gcagtgaatc cgtgaggttc tcatgccaaa
     2221 cttgaatgag caaactttct tttgcgtttt tcaccaaatg ctctacttta tccagaacat
     2281 actcgatgcc atcaactttc cacagcattt cttcttcatc tttttctttg atcatcccta
     2341 gattcttggt aagaaaagaa agatcatttt taacactttt ttctaatttc tctaaaaact
     2401 catttgccga gatggcatga taaagtttag gttctgattt attaacgaca atcaaattct
     2461 tttttaatag tttttccaaa atgttatata ctttcgagcg tggcacacct gagattttac
     2521 ttacttcata tcctgtaaga ttaccttttt ctaatagtgt ggtatacact cttgtttcca
     2581 tctctgaaaa atcatatttt ttcatgattt gaataatcgt ttccattgat taatcctcac
     2641 tttattttta gtagctacta cggtaacaac tataaattct ttttgatgat ctgtcaagag
     2701 ttagttcaat aaaacacata taataattag cttaaaaaca agctttttat taaaaagaaa
     2761 cattataata taaatctttg tgaaaaaata atacaactta gtcattcttt acaacgattt
     2821 acagaaaaac gacctcttta aaaataaaag gtacgggtta ctgattactt gtaaatcttt
     2881 acgttccagt tttattcttt ctaaaaaaac tcctcacttt ttttcttaga cttctttttt
     2941 tgattctcct ctttatggtc ttgtcctttt ttgccttcaa aatatttaaa caaacctctt
     3001 tcaaaagcat ttttaggttt tggacaataa ggaataactt ttttcttcat ataaacacct
     3061 catttaacta tttgatctat tttatatcac cacattatca aaataacaaa tgtattaaaa
     3121 gaaagttttt attaaaacca tcttgacctt ttgagaaatt tcaatgcgat ttctttttat
     3181 tcccatactt tttttgttgg tcactttcat atacccaatc taagcgatca tcggcgacgg
     3241 tgcttttaca acggatggta agacaaaaca aggggcggaa aaactttttt gatcatttca
     3301 taaaaaactt tttcctacag cattcccttg ttttctctta ccacacgttg tgaacccaaa
     3361 gcacgccgac gaacgcttag ctatacgaaa atgaccaacc aaaaacaaaa agtgaggtga
     3421 atagaaacaa aaagaaattg aaatctccca aaaggtcgcc cacctcggag acaacttgct
     3481 gccgaaaatg gttggttgat tcggctaagt atcaaataag gcaaatcaaa cattattgga
     3541 ggaatagatc atgaaagcaa atacaccata caaagtagga gacatttttt atagtagttg
     3601 gggctatgaa caaaccaacg ttaatttttg gcaaattatt aaattaaccg aaaaaacagc
     3661 atggtttcga cccatcaaaa agcaaactgt aaaaacctat acttctatga gtggagaaac
     3721 tatgccaata ccaaatgaat ttatcgatta tccgcttgta cgaaccaaag acagcattgt
     3781 tagaaaacga attaatccag ccttatatcc caatgaatta tacggtaatc gcagttggga
     3841 tacattttac cgctatgata atcaaccggt ttatgaaagc agttattact aagagggaat
     3901 ttccctctta gtcaatgtta aaaataacaa aaataaaagg agcaagaaca atgaaaccaa
     3961 taactctata tgaaatctta ggtgaagaac ggtctgacaa tttaccaaca acgcataaat
     4021 taagagttct tgcttctaaa tctatcaata aaaaatgata actgagtatt tctaatcaag
     4081 gagagattgc aatgctaaga acaaatgcta ttcctatcgt tgattcttac gatcggaata
     4141 ccaataacta tttaggatct tttgaacaaa cagatgaaaa tattttgaac tatgttgctg
     4201 ggttaagtcc ttttcaatct gtacgtttag ttgaacatac aacagataca ctcattttaa
     4261 caacaatcgg ctacttcttc gatcaggtat ctgaccaaca atggttacaa cagattttac
     4321 ctaaacttat cgcaaaacaa actggaaaaa aaaccatcga agcagtcaaa atattttttt
     4381 actaaataca tgtattcatt ctcaagggat ttttcccttg agtgaaagga gaaagaatga
     4441 tggccatact tcaacccttt ggtgaaccaa tcgaacgaac agaattcatt caacactata
     4501 tgaaattatt tgttcaagtt attaaacaca ctcatcaaat tgatgagttt tattccaaag
     4561 aaatagagta tcttctagca gagaaacaaa aaattgcttt actttatgac tattttgtcg
     4621 aaatgtatga tcgcgcacca gattactttt atttatctga tacacttact actaactttt
     4681 tagctaaaga atatcttttt gcctcacaca caaaaaactt tatgtgtgta gaacactttg
     4741 tcaataccta tcttcatttg ttaaaaactc aaaaaatctg tacatttgaa gcatttcaaa
     4801 cagattatct atttatttta gatcgtgaag catatcatgc aaaacaagca tttgaaaagc
     4861 aaaatcaagc cattgaaggc tatccagaat tacgcatcca aaacaattct tttttacaac
     4921 aacgattgtt gaaacagcta ataaatggat tccatcaacg aaataaaggc tatcaaaaag
     4981 atcaataatt gattcttttt gatagttttt ttccatcaaa aacagaaact tttttttaat
     5041 tgtttg
//
LOCUS       DAXWLF010000168         5023 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXWLF010000168 DAXWLF010000000
VERSION     DAXWLF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.171"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            25..696
                     /locus_tag="ACGE4U_002384"
     CDS             25..696
                     /locus_tag="ACGE4U_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8394489.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACGE4U_002385"
     CDS             755..1195
                     /locus_tag="ACGE4U_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394490.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACGE4U_002386"
     CDS             1207..1875
                     /locus_tag="ACGE4U_002386"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFF8394491.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACGE4U_002387"
     CDS             1939..2562
                     /locus_tag="ACGE4U_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFF8394492.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACGE4U_002388"
     CDS             2618..3004
                     /locus_tag="ACGE4U_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFF8394493.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACGE4U_002389"
     CDS             3217..4608
                     /locus_tag="ACGE4U_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFF8394494.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1660 a          835 c         1068 g         1460 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt att
//
LOCUS       DAXWLF010000169         4990 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXWLF010000169 DAXWLF010000000
VERSION     DAXWLF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.172"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACGE4U_002390"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACGE4U_002390"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFF8394495.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACGE4U_002391"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACGE4U_002391"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFF8394496.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACGE4U_002392"
     CDS             2591..3376
                     /locus_tag="ACGE4U_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8394497.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACGE4U_002393"
     CDS             3389..4240
                     /locus_tag="ACGE4U_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFF8394498.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACGE4U_002394"
     CDS             complement(4364..4816)
                     /locus_tag="ACGE4U_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394499.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1689 a          815 c         1038 g         1448 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata
//
LOCUS       DAXWLF010000170         4974 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXWLF010000170 DAXWLF010000000
VERSION     DAXWLF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.173"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            155..673
                     /locus_tag="ACGE4U_002395"
     CDS             155..673
                     /locus_tag="ACGE4U_002395"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8394500.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            801..1484
                     /locus_tag="ACGE4U_002396"
     CDS             801..1484
                     /locus_tag="ACGE4U_002396"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFF8394501.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1793..1866
                     /locus_tag="ACGE4U_002397"
     tRNA            1793..1866
                     /locus_tag="ACGE4U_002397"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1827..1829,aa:Arg,seq:tct)
     gene            2025..2384
                     /locus_tag="ACGE4U_002398"
     CDS             2025..2384
                     /locus_tag="ACGE4U_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFF8394502.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2507..4138
                     /locus_tag="ACGE4U_002399"
     CDS             2507..4138
                     /locus_tag="ACGE4U_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFF8394503.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4200..4841)
                     /locus_tag="ACGE4U_002400"
     CDS             complement(4200..4841)
                     /locus_tag="ACGE4U_002400"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFF8394504.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVPPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
BASE COUNT         1545 a          814 c         1040 g         1575 t
ORIGIN      
        1 caattattta taataaaaaa tgagataaag atgaggaaat agaattagaa acatataatt
       61 attaaaggga aacattttta cagctataat ttgaaaaaaa tttatcgtta ttatatgatt
      121 aagaaggatt gaataaagaa agaagttgaa aacgatacct gattacaaaa aaataacttt
      181 tataagttct ataacgttga ttatattttt aattcttatt cttctcgtcg tcttggttcc
      241 ccgaattttt ggtttgactc cttatattat tgaggacaaa gaaatgagtc catattatgc
      301 acaaggaaat ctgatatatg tacgaccgaa aaatacaaat gagattttag tcggagatat
      361 tattacatat tatgaaaatt caggaaaaaa aatctcgacg cgtcgcgtaa tagccgttga
      421 aaacaaccac gaagattttt atacaaaagc tgatacaaag acttatattg agcctggagt
      481 agtaaaaaag agaaatatta tcggtagtcc catctttcag attccatata ttggactagt
      541 cctttcaaaa acggcatttg catggattca ggggttattc tttacgattg ctttttgcct
      601 aacaggaatc acagcatgga atacctttgt agagttgaaa aagaagcgga gacgaacaca
      661 gaaatttttg taagaatgtt aaaaacataa atgtattcca gagatccaac gactcttctg
      721 aacatgaaac acgagaaaac actcatgcag cttgtagatt tagtcagttg ttcgtataat
      781 gtgtgaagag gaggagtagt atgcgcaatg aaatgatgca tcgtcctgta gtcaaagaag
      841 aactattgga ttttatgcgt acccagcaaa aaaaattacc gggtgagcta ggcaaactgg
      901 aagaagaagc tcatgtagca gaagtaccag ttattcccca tgaaacagtt gtatttttga
      961 aatttttact tggacagctt cagcctgaac gaatcttgga gatcggagca gctatcggtt
     1021 tttcatcaag tgtcatggcc acgacaatcg gagaaaacgc tcatgttacg acaattgatc
     1081 gtttcgatgt catgatcgag aaagctaaaa aaaactatga acgtctagga ctgacagata
     1141 aagtgacttt gcttgaaggt caagcagcag atatcttgcc ggagttgtct ggtccgtatg
     1201 actttatttt tatggatagt gcaaaatcaa aatatattga atttttacct gaatgcttac
     1261 gtttgttaag aaaaggtggc gttttgatgg tagatgacat cttccaagga ggaacgatct
     1321 tgctcccaga tgaagagatt ccccgtggca aacgagctat ccatcgtaaa ttaaatgaat
     1381 ttcttcgtgt tgtgatggat catcccgatt tgacatcgac aatcttgcca ttaggtgatg
     1441 gtgtgatctt aatgacaaag gaatcagaaa caatcgagtt gtgatacatg attatggcag
     1501 cctgtagata atcgattgaa tcgagggctc aattcgaact tgatgtaaaa gtttctatat
     1561 ttgtaagtca attaaaggat acaggaaatc taaataaaca ggagtagcat gaagtcaatg
     1621 tagctcctgt tttcaattga taaccttaga acagctgaag gagaatgtct cgttttaaaa
     1681 gattgattta gtagttgggc caaaataatt cattatttgc taattaaata actatttcgt
     1741 attgacagaa gcaatttaat tatctataat tattgatgtt ccgctgattc ttgtcatagt
     1801 agctcagctg gatagagcat ccgccttcta agcggacggt cgggggttcg aatccctcct
     1861 gtgacgctaa aaaaccgtta gaaactgagg tttctaacgg tttttctttg ttttttgtgc
     1921 caatattgtt tgttttcaaa ggcaaataaa ataaaaaaac atccttatcg agtacaatta
     1981 taaggagcaa aaaaataaaa ctcatcagaa aagaggaaga agatatgacg atactatcta
     2041 gaattttagt gactctagtt gcaattgaat ttttttatat catgtatatc gaaacggtga
     2101 gaactgattc tgacacgaca agtagggttt ttaaaatgag taaagaagag ttaagcagaa
     2161 aatctgttca aacattgttt aagaaccaag gagtgtacaa tggactatta ggagtgggac
     2221 tactttatgg agcttatctt tcttccgcat ctaaagaaat cacaagtatg ctattaatat
     2281 ctattttttt tgttgcatta tatggcagtt tgactagcga tcgttggatt ttactcaaac
     2341 aaggtgggct agctctgttg gcattgcttt ctattttctt ataaatatag aaaaaataag
     2401 aatttatcta acaggaaact ttgtgaaaaa tattacttta tctcatgaaa tatgatattc
     2461 taatgaatgg tattttagaa aatttcatga aaaaggagtt agataggtgt tcaaaaatta
     2521 tgtttcctca ctaaaaaaag aattttcagg ttatgatctt tcaagatttc agaaagatct
     2581 tttggctggg atcacagtag ctgctgtagc attgccttta gccttggctt ttggggtatc
     2641 aagcggagct gatgcggccg caggtttgat tacggctatc attgccggta tcgttattgg
     2701 cggtttatca ggcggttttt atcaaatatc aggtccgaca ggtgcgatgg cagctatctt
     2761 gatgtctatt gcggcaacaa agggaatgca aggtgtattg cttgctactt ttttagcggg
     2821 tctattttta cttgcagcag gacttttccg attaggaaca ctgacttctt ttatcccagc
     2881 acccgtaatt acggggttta cttcaggtat tgctatcatt attgcattag gtcagatcga
     2941 taatttattt ggtgttcact cggaaggtac aaacgttatt gaaaaactaa gtagttatca
     3001 aacgatagga tttccgataa acacagcctc tttgatcatg ggagggctag tcattttagg
     3061 gatgtttatc tatccaaaaa aatgggcgaa acgaatgcct agttcgctgc tggctatcat
     3121 tttggcaaca gcagttatgc tattgttcca tttaccagta gcaactgtgg gtaagattcc
     3181 acaaacattg atcagtagta atcgtttgaa tatgggagat tttagctttt ctgccttgca
     3241 acaagtggca gttccagcta ttagcattgc tttattagga atgattgaaa gcttgctatg
     3301 tggtgcttcg gctggccgaa tggcaaataa gcccttagat agcaatcaag aattaatggc
     3361 gcaaggaatt ggaaatctgt tgcttccttt ctttggcggg attcctgcta ctgcggccat
     3421 cgcccgtaca agcgtggcca tcaaatctgg tgcacaaact agattagctg gtatgataca
     3481 tgcagttgtc ttactgctct ccatgcttgt ttttgcgcca gtgatgtcac agattccaat
     3541 gccagcactt gcaggtgttt tgattgtgac ggcttggcgt atgaatgaat gggaaacgat
     3601 caaggagcta tttactaata aatattggtc agcagttcta ttgtttatcc ttacgatgat
     3661 ttgtacagtt atctttgatt taagtattgc tatcgtgatc ggggtgatcg gcggctgtgt
     3721 attttttgtt gtcaaaagcg ctgctatcac gatttcatta gaaacgatcg attggcaaag
     3781 aatggggctg ccagaaacgg aaaagctgaa taactggaca gtagtgtata ttagtggtcc
     3841 actgtttttt atgtcttcag aaaaactcaa aaataaattg gaagaactaa atcatcacga
     3901 tggtgtcatt ttctccatgc gaggcgtacc aagtattgat ttgacggcac gtgcagtttt
     3961 tgatgaattt caagaaaaag cagagcaaag aggacaaacg attatctata cttccttgca
     4021 gccagaagta gaaaaacaac tttctcactt gtgggaacat acattcacgc atcaacatca
     4081 aacagttgct catgcgttga cagctttaca tgcgaaatat actgcaatta gccattaaaa
     4141 caatgaaaaa gaacagacaa gactgtaaac tgaagatttt atggtcttgt ctgttctttt
     4201 taatattgct gtaaaagtga agctagtact tgccagtctt gatggataac aggtacaagg
     4261 gagcgattgt agaaaatagc tgctggatga aaaagtggaa gaattcgata gtttttttct
     4321 gaccattcgt aggcatcctt ttgttctgtt aattgtaaga caggccctgt atagagttga
     4381 ccatgatttt tggtaaccgt atattctttg ccaagtaaac gttgaagacc aatgtttcca
     4441 agtgtcgcaa ttaattttgg cgggaccttc atcagttcgt aatccaatat tggtgcatgc
     4501 gcaaatactt cggaacgagt aggtgtccga tttggataaa cagtttctga ctgttgtgtc
     4561 cgtttattga tccgatgagt cacgcgatac gggcggctcc ttacagcact ggtgatatac
     4621 acctcttctc gtgttaagcc agttgaagaa agtgcctgca tcaattcttt tccagcttga
     4681 ccgctaaaag gaacatgcgt atcaatctct gttttccctg gggcttctcc cacgagcatc
     4741 agtttaggat gaagaggtcc ttgcccagcg ataaatcctt ctacaggacg gccgttcatt
     4801 cttaaagaag cttcttgtat cagttctttt gggtattcca tttatgctcc tcctttttct
     4861 tttattataa gaataaaaga aacacaatgc gactatttta tttggtagaa ctgtgaggat
     4921 agaaaagcag aagtattgct tgaatttttg ttttactttt tgattttaaa ttta
//
LOCUS       DAXWLF010000171         4971 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXWLF010000171 DAXWLF010000000
VERSION     DAXWLF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.174"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..442)
                     /locus_tag="ACGE4U_002401"
     CDS             complement(<1..442)
                     /locus_tag="ACGE4U_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFF8394505.1"
                     /translation="MTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISV
                     FGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCI
                     FTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
     gene            complement(500..1552)
                     /locus_tag="ACGE4U_002402"
     CDS             complement(500..1552)
                     /locus_tag="ACGE4U_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8394506.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1565..2503)
                     /locus_tag="ACGE4U_002403"
     CDS             complement(1565..2503)
                     /locus_tag="ACGE4U_002403"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8394507.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2954..3604)
                     /locus_tag="ACGE4U_002404"
     CDS             complement(2954..3604)
                     /locus_tag="ACGE4U_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394508.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(3704..4213)
                     /locus_tag="ACGE4U_002405"
     CDS             complement(3704..4213)
                     /locus_tag="ACGE4U_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFF8394509.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(4576..4959)
                     /locus_tag="ACGE4U_002406"
     CDS             complement(4576..4959)
                     /locus_tag="ACGE4U_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8394510.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
BASE COUNT         1576 a         1035 c          729 g         1631 t
ORIGIN      
        1 tttgacgata aagcagaaca agtaacaatg ggaaaagtag aaagcctgca tagcctagcc
       61 cagaacctgt attatggatc caagtcgata gattccaaga gctttcattc gtattgatac
      121 tgaataaccc agtaaaaata caatcaccta tcccatataa accaactgtt gcaacagcaa
      181 gtattgcaag tgtttttgat gttccaacaa ataggtgata caacgcaggc aacgaaagaa
      241 caaaaaaaag tccagataca actgaccaga ccaagaaagc acgtctgact ggactatcta
      301 cgtcaccaaa cacactaatt acggtagtca tttgattcag tttgggataa aaaaggccaa
      361 ggatatacgg tgtcgataaa tcactaatca cacctaataa taaaaaataa aaaccatatt
      421 ttggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa
      481 aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt
      541 taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc
      601 aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa
      661 gtccgaccac accgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag
      721 gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata
      781 gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat
      841 taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg
      901 caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag
      961 agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg
     1021 tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata
     1081 aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca
     1141 ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca
     1201 tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag
     1261 gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca
     1321 tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctaatt
     1381 gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta
     1441 aaatagcacc taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag
     1501 cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc
     1561 tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac
     1621 aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat
     1681 cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc
     1741 caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt
     1801 caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat
     1861 gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt
     1921 tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat
     1981 gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc
     2041 catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac
     2101 cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt
     2161 tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt
     2221 tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa
     2281 taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg
     2341 cttctacctc tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat
     2401 gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc attaacccaa
     2461 agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt
     2521 tctttctatt atacttattt gagaatcatt tcacaacctt gttttgttat tcttctctta
     2581 tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa
     2641 tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca
     2701 tagaaaaaac ctcactcctc tgattataaa agaggggaca ttaacctcgt gtatactaaa
     2761 accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga
     2821 tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa caatacactt
     2881 atttaaaaac ttctttccca gattttcttc catttttaca gttataacta gtattatcat
     2941 gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact tttttattaa
     3001 ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctgactca accggaatta
     3061 tccaagccca gtccaaataa ttttttaaaa tctcatcata ttttgcaaat atttcattgc
     3121 catattccgt ccttaaaatt atattttcag tgccagacaa gtgatttgta ttgagttgat
     3181 aaaaaagatc tccatctaaa aaaatatcat tagaagaaga tttccccaca gtaatgacca
     3241 ttccatcagc tctatgtgtt acatacaaat aattgtatga cctcacttca cctaatgaat
     3301 caattatttc ttgcccttta gctctaatat attgatctct attacctatc tcactcttat
     3361 ttaacaaaag ttttttcatg tcaaatttaa tcggctgttt tatttttgta aattcgcttc
     3421 tactactaat ccaatccgag attaattttt caacatgtcc cagagtaaac tctgatttat
     3481 tcgctaaata ctttttttgt tgtattaaag acatctttcc ccgaagctca aaattgagcc
     3541 tacatcgatt ctctatctct tcaaaaagca tcttatcttc atcattctta tattcatcat
     3601 tcataaaacc aatccagctc ctcgattaag ataatatttt aatctcatac aaaaactagc
     3661 tctcatcaaa acaaccattg ttttgatttc tcatgaaatt tctttatact tttctccgat
     3721 tataaattcc taaccccttg agaaaattac gcatatattt atctccgcaa ggcttaaaat
     3781 ttcgttggcc ttcttttctc aaaaatgccc cgatttcgcc ttttgatgga tagagttctg
     3841 cctcagcaaa aacttctaaa atatcatcag tggtcataga caaggctatt tttaattttt
     3901 ttagcaacac attgttgata tatttgattt ccttgctttt ttgcggcgtg tctactatcg
     3961 gcttatcttt ttgcggacca cgcgccaaga gaatcaggcc atttaggaat gcgtcaaaaa
     4021 cttggttgtt gatggttttt tcatacacat tattttctgc attatctttg ttcttatcct
     4081 gtactttggt cagtaccgcc tgagcttctt ctttagtcag tggtacaccg cctagctcaa
     4141 aagctttaat caaatccgta tctttcaaat caatcgcata acgcaatcgc aataacctat
     4201 cattgttatt catttgttcc tccgaagtat ttagtcttta ctttcttacg tactttttgt
     4261 cttgctaaga tttttaggat caatgataaa aaccgcatca cttatgatac ggagctgggt
     4321 atcatgagtg aatgatattt ataaatcact ggatattgaa gtatattctg ttttctcaac
     4381 ttatttagtc agatttttta atgaagattt tttgagtaaa atttctataa ttcctactta
     4441 ttgaaaagta gtttgtagtg ctgcttctac ctctctcatc tgttctacaa attccaaata
     4501 acttgacgca gcttgcggaa tgaaaaaatg atatgtgaat atttctcctc ctagcccgct
     4561 taatatgcct attttttata cccatgattt gcaggaaggg gatcggaagt aggttgcaga
     4621 aagccgcctt tcataatctg tcggaaaccg gacatcaaaa cggattattt tacttgtcac
     4681 atttgtattt tcttgtgagg aagtgccaaa tacattattt acttgagtga cattctcatt
     4741 gtcctgtctg atattgcttc gtataaaagt accagatcca cgtctcgtat ataacaaacc
     4801 ttcatcaata aggagttgta tagctttgcg gatagtaaca cggcttgttt taaactctct
     4861 agccagactt tcttgctcag gtaatttaga accttcctga tattttccct caacgatcct
     4921 ctcttttaga gccaaggcta tttctttata ttttggcata ttttcccctc a
//
LOCUS       DAXWLF010000172         4960 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXWLF010000172 DAXWLF010000000
VERSION     DAXWLF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.175"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            96..809
                     /gene="treR"
                     /locus_tag="ACGE4U_002407"
     CDS             96..809
                     /gene="treR"
                     /locus_tag="ACGE4U_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFF8394511.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            886..1332
                     /gene="msrB"
                     /locus_tag="ACGE4U_002408"
     CDS             886..1332
                     /gene="msrB"
                     /locus_tag="ACGE4U_002408"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFF8394512.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            1502..2104
                     /gene="ruvA"
                     /locus_tag="ACGE4U_002409"
     CDS             1502..2104
                     /gene="ruvA"
                     /locus_tag="ACGE4U_002409"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFF8394513.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            2117..3118
                     /gene="ruvB"
                     /locus_tag="ACGE4U_002410"
     CDS             2117..3118
                     /gene="ruvB"
                     /locus_tag="ACGE4U_002410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFF8394514.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            3147..3725
                     /locus_tag="ACGE4U_002411"
     CDS             3147..3725
                     /locus_tag="ACGE4U_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8394515.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            3777..4259
                     /locus_tag="ACGE4U_002412"
     CDS             3777..4259
                     /locus_tag="ACGE4U_002412"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFF8394516.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            4376..4750
                     /locus_tag="ACGE4U_002413"
     CDS             4376..4750
                     /locus_tag="ACGE4U_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394517.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         1702 a          850 c         1082 g         1326 t
ORIGIN      
        1 ttaaaataag cttttcttaa aaataggaac attgaaatac aaaaagagct gtagtatagt
       61 gaagaaatga tggaagaacg agtgagggaa aaaacatgaa taagttcaat gaaatttttt
      121 tagatttgga acagaaaatt ttagcgaaag aatatcctcc gcattcgctt ttacccagtg
      181 agaaccaatt gatcaaaatt tatggtgttt cacgagaaac aattagaaaa gcgctgaatc
      241 ttttgactac ggaaggttat attcaaaaaa aacaaggaaa aggttcgatc gtactggatc
      301 ggaatcgctt cgactttcct atttctggat tgactagcta taaagagcta caggagaccc
      361 aacgtattcc aagtgaaacg atcatccata cgttagaaga aacagaagtg acaaaagcat
      421 tgaacgagat cactggctgg gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa
      481 aaatcaatgg cgaagtagtt atattagaca ccgattatct attaaaggaa atcgttccta
      541 ttttaactcc agcacaagca caaggatcga tctatgaata ttttgaaaat gagctttctt
      601 tgacgattgc ttacgcgcaa aaggagatca cagtagaaga agtaacggaa atcatgaaaa
      661 cagcgatgga tcttcacgat gacacttatg cagtagtagt cagaagcctc gttcatctgg
      721 aagatacccg ctgtttcgag tattctgagt cggttcaccg attagataaa ttccgtttcg
      781 ttgagtttgc ccgacgccga aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc
      841 tgtccacttt ggcgctatga taggatcaca aagggggcat ttttaatgga taaaccaata
      901 agaccagaaa aagaagaact gaaagaaaag ttatcaccca tggcttatgc agtcacacaa
      961 gaaaatgcaa cagaacgacc atttagcggg aaatatgatg atttttacga aaaggggatc
     1021 tatgtagata tcgtcagcgg tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt
     1081 tgtggctggc catcattcag caaaccaata acgaaaagac aagtgcgcga aaaagccgat
     1141 ttttctcatg gcatgcatcg aatagaagtt cgaagcaaag aagcagattc tcatctcgga
     1201 catgtgttta ccgatggacc agtagatcaa ggtggtctac gttactgcat caattctgcc
     1261 tcactgaaat tcattccata tgaaaagatg gatgaattag gttatgggga attcaaatca
     1321 cttgtagaat aatggaaata cacaaagagc ggtacaatac ggggatttac tcgatcgtcc
     1381 gctctttttt tattcaagcg attgctattt gaaagaaacg ctccccagac tccttttttc
     1441 acaaggtgaa atgtgctaca ataaagggaa cgtatgtgtg caaaagaaag aggactagaa
     1501 tatgtatgaa tatctgacag gtgtggttac ctttatcaac ccctattaca tagtagtaga
     1561 aaccaatggt atcggctacc agatcgcact aggaaatcct taccgctaca gtagcaaact
     1621 aaatcaaaaa ataaaaattt atgtccatca ggtaatacgt gaagatgcgc atcttctgta
     1681 tggatttgat tcgctagaag aaaagcaact gtttttgcga ttagtaagtg tttcaggaat
     1741 cggtccgaaa agtgcccttg caatcatggc aagcgaagac cactccggtt tgattcaagc
     1801 agtagaatcc agtgatgtca cttatttgac gaaatttcca ggagtaggga aaaagacagc
     1861 acaacaaatg atcttggatc taaaaggaaa attcggtgag ctgagtatcg atacaccttt
     1921 tagtctattt gatgaagcaa acacaaaaga cgctacagct ctatctgaag caatggaagc
     1981 tctctcggct cttggttaca gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga
     2041 agtagataat cagacaacgg atgagtatct acgccaagca ctcaagctaa tgatgaaaaa
     2101 ataaggagga gaccaaatga cagatgaata cttgttatca ccagaatctg gcgaaaatga
     2161 actgagtttg gaaaaatctc ttcgaccaca gctgctttct caatatatcg gacagagaaa
     2221 agtcaaacaa gaacttcaga tttacatcga agctgccaga aatcgggaag aacctttaga
     2281 tcatgttctg ttgtacgggc ctcctggatt aggaaaaacg acgatggcca tggtcattgc
     2341 taatgagatg accgtaaata ttcgtacgac gagtggccct gctatcgaac gtccgggtga
     2401 cttagtagca atcttgaatg aactggaagc aggggatgtc ctgtttatcg atgagatcca
     2461 tcgattacct agagtggtag aagagatgct ttattcagct atggaagatt tctatgtaga
     2521 tatcatggta ggacaagggc caaccgcaca tccggttcat ttccctcttc ctccctttac
     2581 tttgattggc gcgacgacac gggcaggtat gctttctgca cctttacgag atcgatttgg
     2641 catcatttct catatggaat attatgacga acaggatctg caggagatcg ttttacgttc
     2701 ttcggatatt tttcaaacag agatcattga agaaggggca ttggaaattg ctcgccgatc
     2761 acgaggaacg cctagaatcg ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt
     2821 ccaaggagac gggaaaatcg atcgtcctat agcggatcag gcacttactt tgctacaggt
     2881 cgatcaggct ggattggatt atgttgacca gaaattgttg cgaacaatga tcgagttata
     2941 tggcggcgga ccagtagggt taagtacgct ttcagtaaac ataggagaag agcgtgagac
     3001 ggtagaagat atgtatgaac cgtatctgat ccaaaaagga tttttaaagc gtacacctag
     3061 agggcgaatt gctactgctt atgcctatga acattttggt tacgactatc atatatgatt
     3121 tttgctttta gattggagga aaatgaatga gtgatacaac acgtacgaaa caattgatca
     3181 aaaacgtatt gattgatttg tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa
     3241 tcgcaaatga agcaaaaatc aatcgacaaa cattttatta tcattttaca gataaatacg
     3301 atttgcttcg ttgggtctat tgtgaggatg cattgcatta tttagatcca caatgtcttt
     3361 cacttgataa ttgggaagag caagcattga aagtgctgaa agcaattgct gagaagaaag
     3421 aattttatgc aaatactgtg ctttctgacc cagaaatttt acaaaaagag ttttctcaac
     3481 tgatccaaaa atcatttatc aagatttttg aacaaatgga tgaagaaaga caactttctg
     3541 caagagacaa actattttat gctcgttttt tttcttacgg ttgcagtggt gtattagtca
     3601 attggatcac gaatgaatat gaagaaacac cgatggaaat cgctatgcag cttttccgtt
     3661 tagccaagga tacggaatta tattcttatc gtctttatgc aaaagaagag ttggaagaac
     3721 agtaatagaa aaattttttg ttggaaaagc aataatcgct aaaaggagat ggggaaatga
     3781 caaaaatttt atttgtttgt ttgggaaata tttgccgttc accgatggca gaaggcttgc
     3841 tgagaaaacg aatagcaata gaaggaaggg aagcagaatt ttttgtggat tctgctgcta
     3901 caagcacgta tgaagttgga aagacaccgc accctggaac gaaaaaaata ttaaaccaag
     3961 aaaaagtaga tatgacgaat atggttgctc ggcaaatcac accccacgat tttgaaactt
     4021 ttgattggat cattggcatg gatcaagaaa atgtagaaga actgaaacga agagcaccac
     4081 gctcagcgca ggggaaaatc catttgtttt taagtagcat tcctggaaaa gaaaaagaaa
     4141 atgttcccga cccatactat accaataact ttgatgagac atatcacttg atcacagaag
     4201 gcctgaatca ttggtggaat atttggattg acgaaaataa aataacaaac aatttgtaaa
     4261 gttgacgctt tctttttaag gcgatgttag agcatttcct tgtttttttg taaaatgtta
     4321 ggtaaaataa aattaattaa gtaacacttt cttagctaaa ggaggagtgc attcgatgaa
     4381 cgaagagaca gtatcgccaa tgaagaagct tacgaagtta gttactcata cttcgtatca
     4441 actttttgat tcttttgtaa atgacactga agaacatgct aagaatgtac gattgcaagg
     4501 aaagtttatt cttgagctgc aaaaagctgc tactgaacat agtctagtta tcttacaagt
     4561 aagagcgaat cgtttgagta gcaagtatga aacgatctca ggctgggtgg cgacaaaaac
     4621 ggtcgaaaaa gaccgagtga tgatccgcct acaaaacgaa gagcagcaga tccggatggt
     4681 tcctgtagaa caaattgaaa aaataacaat cctttcacca gcaggggaac aggaacgatt
     4741 atcaaaataa tttataccag atagaaaagc acgtatgagc ttgtaccatc aatcattact
     4801 aatcaaaaat aaccgaatgc tttaagctga tatcagtaaa acggaacgtt ttaggatgag
     4861 tatcacagca tgacagcatt gggttgatta gttgaatcga ttggatggta caagtttttt
     4921 tgataaaaaa aggtcctgct agccggggag ctagcaggat
//
LOCUS       DAXWLF010000173         4913 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXWLF010000173 DAXWLF010000000
VERSION     DAXWLF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.176"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            16..1305
                     /locus_tag="ACGE4U_002414"
     CDS             16..1305
                     /locus_tag="ACGE4U_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFF8394518.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            1318..4020
                     /locus_tag="ACGE4U_002415"
     CDS             1318..4020
                     /locus_tag="ACGE4U_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFF8394519.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            4005..4862
                     /locus_tag="ACGE4U_002416"
     CDS             4005..4862
                     /locus_tag="ACGE4U_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8394520.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
BASE COUNT         1734 a          850 c         1068 g         1261 t
ORIGIN      
        1 aagaaggttt tattagtggc ttataaagat ataccaaaat cggtgcagaa atttatggaa
       61 gatatcacac aaaaatgtgg ggatatccat gcggactggg caaagaattt tacagcagca
      121 tttgccaata ctctgttgac aacagtcaaa aagcaaaatg acggaaccac ctttttattg
      181 acgggagaca ttccagcgat gtggttaaga gactctactg cacaagttcg tccttattta
      241 gtgattgcaa aagaagatga ggaattagct gacatgatca gcgggttagt caaaaagcaa
      301 tttttttata tcaatatcga tccatatgcg aatgccttca acgaagaagc taacggtgct
      361 ggccaccaaa cagatcatac aaaaatgaac gattggatct gggaaagaaa atatgaaatt
      421 gattctttat gctacccaat acagttggcc tatcttcttt acaaaaatac aggaaaaact
      481 gatcaattca atcaatcttt tattgaaggt atcaaaaaaa tcttggacgt gttcaaaaca
      541 gagcaagatc atgaacactc tccttataca tttgtacgag acacgacaag aaaagaagat
      601 acgctgatca atgacgggcg aggtgcaaaa acagctttta caggaatgac atggtctggt
      661 ttccgtccta gcgatgatac ttgccgttat ggatatctgg taccatccaa tatgtttgcg
      721 gtggtcgtgt taggttatgt ggaagaaatt tttcaaacga tactcgatga taaagaaatt
      781 gtacagcaaa cacagaaatt gaagaaagag atccaagagg ggattcagaa tcacggacga
      841 acggttaatc aaaaagggca aacgatttat gcatatgaag tagatggaat gggaaacgcc
      901 acagtcatgg atgacagtaa tgtgcccaat ctgatcgccg caccttactt gggatattgt
      961 tctgaacaag aggaagatta tctgcaaaca agaagaacat tgttgagtaa agaaaatcca
     1021 tatttctatg aaggaaagta cgcaaaagga attggatcct cccatacgcc agaaaaatac
     1081 gtttggccga ttgcattagc tatggaagga atgacaacgt cagacaaatc tgaaaaagag
     1141 cggatattga atttacttgt agcaactgac gcaggaacac accttatgca cgaaggattc
     1201 gatgtgaaca atcctgaaaa ttatacgaga gaatggtttt cttgggcaaa tatgatgttt
     1261 tgcgaattag tcatggatta ctttgatatt agagtagaaa agtaggaggt atcgtaaatg
     1321 aaaaagaaag tatatatcat ttctcatagc cactgggatc gtgaatggta catggcttac
     1381 gaacagcatc acatgcgact gattgaattg atagacgatc tactagagct atttgaagtt
     1441 gatccgtcct tcaacagttt tcatttggat gggcaaacga ttattttaga tgactatctt
     1501 caagtaaggc cagaaaaaag acaggcaatc caacaagcaa tcaatgaagg aaaattgaga
     1561 attggaccat tttatatctt gcaagatgat tttcttatca gctcggaatc gaacgtgaga
     1621 aatatgctga ttggcatgga agaaagccgt aaatggggaa cccctgtcat gttaggctat
     1681 tttcccgata catttggaaa tatgggacag acgcctcaat taatgaaaca agctggtatt
     1741 tcagcagcag cgtttggccg tggtgtaaaa ccaattggct ttgataatca agttttggaa
     1801 gcagaaaact attcttccca atactcagag atgtggtgga aaggacctga tcaaacagca
     1861 atctttggtc tgctatttgc caattggtat agcaacggga atgagattcc agtagaaaaa
     1921 gaagcagctc ttgccttttg gaagcaaaaa ttagcggatg cagaacaata tgcctctacg
     1981 aaccacttat tgatgatgaa tggtgttgat catcaaccgg tacaaaaaga tatatcaaaa
     2041 gcaatccatc tcgcaaatga actgtttcca gattacgagt ttattcattc taattttact
     2101 gactatctgg aagcagttca aaaagatgtg ccagaagact taggctcagt agaaggagaa
     2161 ttgacaagtc aagaaacgga tggctggtat actttagcca atacagcatc tgctcgggtt
     2221 tacttaaaac aatggaatac caaggtccag cgacagctag aaaatattac agaaccactt
     2281 gcgacgatgg cttatgaagt tagtggaaac tatccccatg atcaattaga ttatgcttgg
     2341 aagacattaa tgcaaaatca tcctcacgac agtatttgcg gttgttcagt cgattctgtc
     2401 catcgggaaa tgatacctcg ttttgaaaaa gcagatgaag ttggaaagta tcttgcacaa
     2461 gattccttag aacagctgac cgcagcaatc gatacgaccg gattcccaaa agacagcttt
     2521 ccatttgtca ttgtaaatac tgcaggaatg gataaaactg gtgaagcaga aataaccatc
     2581 gaattagaaa gaaaaagatt cgctgaagga atacctgaac aactatatca agagttagaa
     2641 aatttgccta aacgtaagta tcatgtagaa acaaaaagtg gggccactat accagctatt
     2701 ttatctgaag aaactgtcca atttggttat gatttgccga aagaccgttt ccgtgtgcca
     2761 tatatggcaa gaatgatcaa agtgacttta ccattagaaa acatgccagc attttcttgg
     2821 gaaacattcg cgcttgtaga aggggaagcg aaagcagaag aaaaggaaac aatgattcat
     2881 cagagtggac gaatcatcga aaacggccct ctacatttga cgattgaaaa aaatggtacc
     2941 attacaatgg aagatcgcaa aaacaaacga aagttgaata atctgcatat ttttgaagat
     3001 atcggcgata taggaaatga gtatatcttt aaacaacctt tctgtgatca gccaatccta
     3061 tccagtaaca aagaaaactc tgaagtaaaa gtattagtag atacaccgga aatagcaaaa
     3121 ataagtattc tacaggaaat ggaaatccct gtttctgcag acgagcgttt agaaaaagaa
     3181 cagcagatgg ttgtggaatt tcgttatcga aaagcagaac gatccaaaga aaaacgaatc
     3241 cttcaaataa aaacaatcat gacaatcaga aaagatagta aaaaaatcga ctttgaaacg
     3301 actctcgaca atcagatgaa agatcatcgc ttacgtgtct tgtttcctac aaagttacat
     3361 gtggaacatc acgaagcaga tagtattttt gaagtggtga agcgcccaaa ccatgtgtct
     3421 aaaagttggg aaaaccccac gaatccgcaa catcagcaag catttgtcaa tattcatgat
     3481 gaagagtacg gtgtaacagt cggaaacttt ggtttgaatg aatatgaagt aacagaagac
     3541 ggacaaatcg cagtgacgct acttcgcagt gtgggagaac taggcgattg gggatatttt
     3601 ccaacaccag aagcacagtg tctaggtgag catcggttca attacagcat tgaactacat
     3661 ggaccagaag aaaaattctc tacttatttg catgcatacg cagcacagat tcctttttcc
     3721 actcaacaaa tcaaacatca tgaaggaaca ttgatttcaa aacaacaata tttaacgata
     3781 aaaagtgaaa cgtttgcaat tacagctttg aaacgcagca agtttagtga taaagtagta
     3841 gtaagaggtt tcaatatgtc tagtcattta gaaaaattgg aaataactaa agacaatgga
     3901 aaaactgtca tattgaatct gcttgaagaa ccaacgaagc aagcggttgt cccaataatc
     3961 caaccttatg agattcggac aatcggattc gaggaggaaa acgaatgtat ggcggaatag
     4021 aagcaggcgg aacaaaattt gtgtgtgcgg tttcagatgg tggagaaatt ttagaaaaat
     4081 taagtatccc taccacaaca ccagaagaaa cattggatca ggtatttgaa ttttttgatc
     4141 gttatgaatt gaagggaatc gggattggct cattcggccc gattggtatc gacaaaacaa
     4201 aagataatta cggatacgtt ttggcaactc ccaaaaaagg gtgggcacaa ttcgattttc
     4261 ttggcacaat caaggaacgc tacaatattc ctgctgcttg gacaacggat gtcaatgctg
     4321 ctgcatatgg ggaattgaag caaggagcag cacaaggaaa aaatagttgt atttacctta
     4381 ccgtgggaac aggaatcggt gcaggtgtcg tgatcaatga agaaattttt tcagggatcg
     4441 cacatcctga aatgggacat atttgggtga aacgccatcc agatgaccat tatgaaggta
     4501 cttgtcctta tcataaagat tgtttagaag gattagcagc aggaccgtct attgaggcac
     4561 gcacagggat caaagggcaa gatctgccgc aagatcatcc tgtttgggct attcaggctt
     4621 attatattgc tcaagcactg atcaactata cgttgacgtt agcacctgaa aagattattt
     4681 taggcggtgg ggtaatgaat caggatcatc tccttcaaaa aatccgtcag caatttgttg
     4741 aattgatggg tggttatatg gaaacacctc aagtaagtga atatatcgtt cgttggggac
     4801 tgccgaatga aagtgggatt atcggtagtt tgctgttagc agaacacgaa catcaaaagt
     4861 aagcagacaa attacacaaa aagaacagag tcttatcatt atctgctgat aag
//
LOCUS       DAXWLF010000174         4786 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXWLF010000174 DAXWLF010000000
VERSION     DAXWLF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.177"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..813
                     /locus_tag="ACGE4U_002417"
     CDS             <1..813
                     /locus_tag="ACGE4U_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFF8394521.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACGE4U_002418"
     CDS             786..1229
                     /locus_tag="ACGE4U_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394522.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            1598..1726
                     /locus_tag="ACGE4U_002419"
     CDS             1598..1726
                     /locus_tag="ACGE4U_002419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394523.1"
                     /translation="MNTFSSERIRHDKAETVYFSFVMACADFFKSLGTRANIFIKL"
     gene            2401..3369
                     /locus_tag="ACGE4U_002420"
     CDS             2401..3369
                     /locus_tag="ACGE4U_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8394524.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNRALELANRQTQHEKETTSLQERIKEQKRKLELL
                     QQLEELEKQQKNRENETDKQKLDNNSDIPLELRKDLLDNLQNLFKD"
     gene            3379..3849
                     /locus_tag="ACGE4U_002421"
     CDS             3379..3849
                     /locus_tag="ACGE4U_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8394525.1"
                     /translation="MTDKSIKEFAEELGVSKQAIRKYFDKLPTKLTPKLIGGKYVISA
                     KAQEFIKNSINQINEVDTEVDSLVDSILIAELKEKNKQIDHLQGVIEIQQKLLDQQQQ
                     LTLQANQQIDKLQKRLQLVYTEETSENESTTLSEETESIEKQTKKKQKWWKLWK"
     gene            3865..4035
                     /locus_tag="ACGE4U_002422"
     CDS             3865..4035
                     /locus_tag="ACGE4U_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394526.1"
                     /translation="MTRKKLIFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            4499..>4786
                     /locus_tag="ACGE4U_002423"
     CDS             4499..>4786
                     /locus_tag="ACGE4U_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFF8394527.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSIT
                     SILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTV"
BASE COUNT         1824 a          772 c          792 g         1398 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcgatta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acatgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgaa
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 ccttttagtg agttttgtag agagtgacct tataggcact ctcacattac gaacgatacg
     1501 tccctcggac gtactgaaag ggtgggactt atgagcgaac taatgcaaaa tttggctagc
     1561 aaaaaagcga aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aaacagttta tttcagcttt gtcatggcgt gtgccgactt
     1681 ttttaaaagt ctaggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaagg
     1741 cgtttcaaat gtctaagtat ataaatatat aagaatagag ttaagagtgc ttaaatcgaa
     1801 ttttagaggg aattcagaca attttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acagttttag caaaggagcc ggaggcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcaactata gggagtaaga
     2041 ttcccagttt agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctgta tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacaga
     2341 cattttgtat ttcataacgt ctgtatctct tgtgcgagta agaaaggagc tattttaaat
     2401 atgtctaatg aaatcgtaaa gtatcatcat gaattaaata caattccgtt gcgaaaattt
     2461 acaccagtcg aaatgaattt gttcttctct atcgtatcaa gaatgcgtga tgtaggtgat
     2521 aaaaaagtac aatttacatt cgagcaatta aaagatttaa gcaattataa agcaactgca
     2581 aatgttcgat tcatagatga tttagaaaca acgtatgaca aattaatgga tttacgtttt
     2641 ggtagaagaa gtgctgacgg tttgcaacgt gaacgttttg ttttatttaa tcaatttaaa
     2701 atcgacggaa aagctgatat tccttttgct gaaatacaag ttcatgaaaa agccttaccc
     2761 ttacttaata atcttgaaga atgggtgcgc tattctctcc aacaattcaa tgaattagaa
     2821 agcagttatt caaaaacaat gtttcgatta ctaaaacaat acagaaccaa aggtttcgct
     2881 tatttttcaa aagaagactt tcatgaatta cttgatattc ctaaaagtta caaccaaggt
     2941 acgatcgatc aacgtgtgtt caatccaata cgacaagaac taaccgcaat ctttaaaggt
     3001 ttaacaatca aaaagaaata cggaaaaggc cgtgggaagc ctgtcattgg ctaccaattc
     3061 acatttaaac cagaaatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
     3121 gaacaaaatc gggctttaga actagctaat cgacaaactc aacacgaaaa agaaactact
     3181 tcattacaag aaagaattaa ggaacaaaag cgaaaattag aacttttaca acagttagaa
     3241 gagttagaaa aacaacaaaa aaatagagaa aacgaaacgg ataaacaaaa gttagacaac
     3301 aattccgata ttcctctaga actaagaaaa gacttacttg ataatcttca aaacttattt
     3361 aaagactaag gagtattcat gacagataaa tcaataaaag aatttgcaga agaactaggt
     3421 gtcagcaaac aagccattag aaagtatttt gataaactgc caaccaagtt gacacctaag
     3481 ttgataggtg gaaaatacgt aatatcagca aaagctcaag agtttataaa aaatagtatc
     3541 aaccaaataa atgaagttga tactgaagtt gacagtttag ttgatagtat tttaattgca
     3601 gaattaaaag aaaaaaacaa acaaattgat catttacaag gggtcataga aatacaacaa
     3661 aaattactcg accagcaaca acaactcacc ttgcaggcaa atcaacaaat cgacaaacta
     3721 caaaaacggt tacaattagt ttatacggaa gaaacttcag aaaatgaatc aactactctt
     3781 tctgaagaaa cagaatcgat agaaaaacaa acaaagaaga agcaaaaatg gtggaaacta
     3841 tggaaataac tggaggaaac attcatgaca agaaaaaagc tcatatttgg ttctatttta
     3901 ctaggaattc taggcttcag tccgatgtta ttgtcagtga tcaataaagg aaataaacat
     3961 ggtgaattga aattgaatcc aaattattac aacagtccta acgaagagtt atttgtaaaa
     4021 gaaacggata aataataaaa aaagactgct ctaacgagca gcccaaaatg agattatcct
     4081 agtttagcaa cataaaccat ttattagatg aaaatgagat acaaacaaag cgtatcaaat
     4141 cctctcaaaa ataacaagtc tttaaaaaat gacagataaa aatgcacaaa gaattagata
     4201 tagataaccg cgaaaaatca agctttagaa agttgctttt agctagacca attaaaaatc
     4261 acaaagtgag cgtctctatc cattggtaga ggggctactt ggcatagtgt acattatgtt
     4321 aactaacgct ataaataacg attatgttcc caaaacaata ttttatgaac tataacaaaa
     4381 atttgttatc gcatattgta tattttaatt ataggtgtat accacttaaa tttaacacaa
     4441 aggcgtgatt attttgaaac ccaaaaagaa aatttatcta ggtctcctct tttccttgat
     4501 gattggaatt tttagtatag gaattacaaa tacctatgct tcagaaaatt ctaatttcaa
     4561 tatcaactcc caaccaattg aagaacaagc aattaatttt gcgaaaactg attttgaaca
     4621 ctccataact tcaattcttt taatggaaaa taacaccgaa actgatatta actatactct
     4681 tggttcgcca tttaaaatgc tcgatggaga aaatacggca aatagttcta ttttctttcc
     4741 agtattttca gacgctgata atacgattaa atatatttat accgtc
//
LOCUS       DAXWLF010000175         4731 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXWLF010000175 DAXWLF010000000
VERSION     DAXWLF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.178"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACGE4U_002424"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACGE4U_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFF8394528.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACGE4U_002425"
     CDS             complement(2064..3062)
                     /locus_tag="ACGE4U_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFF8394529.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACGE4U_002426"
     CDS             complement(3288..3941)
                     /locus_tag="ACGE4U_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFF8394530.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACGE4U_002427"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACGE4U_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFF8394531.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1388 a          995 c          742 g         1606 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat c
//
LOCUS       DAXWLF010000176         4685 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXWLF010000176 DAXWLF010000000
VERSION     DAXWLF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.179"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(114..1076)
                     /locus_tag="ACGE4U_002428"
     CDS             complement(114..1076)
                     /locus_tag="ACGE4U_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8394532.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1078..2517)
                     /locus_tag="ACGE4U_002429"
     CDS             complement(1078..2517)
                     /locus_tag="ACGE4U_002429"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFF8394533.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2702..4648
                     /locus_tag="ACGE4U_002430"
     CDS             2702..4648
                     /locus_tag="ACGE4U_002430"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFF8394534.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1481 a          803 c          775 g         1626 t
ORIGIN      
        1 aacttttggg ggtcactaca agttgacaac tcctttttgt ttacaaatca attggatatc
       61 tacaaattgt attttctaac gaattttata gacattaaaa aattaattta acgttaaata
      121 cttcctccag aaagtattga gattggtaaa atataatcct tatttgtctt ttcgccttta
      181 atttttttca gaagaatatc aacaataaga cttgcaattt catcaattgg ttgtcttatt
      241 gttgtcaatt gtgggaaaaa tttctcaata aaagaggtac catcgtatcc aataattttt
      301 aaatcttctg ggatattaag tttaagttga tgagcaattt tcatagtgag aatggctgtt
      361 aaatcatcag aaacaaaaat accatctggt ttgtgattta taatagttga ctttatttcc
      421 atttcacgac ggatagtaga cagattatta ggaattttga aaatttttcc atttggattt
      481 tgaaaagaaa aacctagtgc acgtaattct gtgggagaat ccgtgttatc atttcctgta
      541 atcataatgg tattttgaca accatttttt tgtaaagtct tagctgcaag ttttccccct
      601 tcaaagttat ctgaagaaac aatggaaaca ttaggagcta aatttctatc aaaagcaaca
      661 ataggtgctc tcacacgctc ataatcagca attccaagat tatggcttga tgagattatg
      721 ccatcaactt ggttggcttc aagcatttca atgtattctc tctctttagc aggatcattt
      781 tgactattgc aaataatcgt tttatatcca tgtttaaata actctatttc gagatactca
      841 atgagttcag agtaaaagat attactaata ttaggaaaaa taagaccaat aagctgaggt
      901 gactttcctt gaagtccacg tgctaaatta tttggtttat aaccaagagt tttcatagct
      961 tcatgaactt tttgttttgt tttttctgat aaatatccct tattattaat gacgcgtgaa
     1021 acggttgttg ggctaactcc agctaattca gcgacatcag ttaattttgc taccatttta
     1081 atattttaat tcaaagtatt ttccagtagg ttcgcctgat ttgatttgaa taccattttg
     1141 gtgattttca gggaatacac gactagtaaa cactttttct cctttattaa taaaaatctc
     1201 aatgattgag ttatctacaa aaatgttagc agttgtttct ttagtatcaa ttgagcattc
     1261 acgaatcgta ccaaagtcag tggcatattg aatgcctgct ttgctgcgat ctaaaataat
     1321 ttttccttct ttggtatcta caatcaagct aagtccattt cctttactat cagagaaaag
     1381 aagtaactcc gatttctgat ttggaggaaa agttagttca agctcataag tattatttgt
     1441 ttctgctttt gctgtaaatt gttcagaact agcacgtaat gatgtaatag cttctactgg
     1501 atattggtaa agtttaccat cttttattga aagttctttt acaagactca ttgctccctg
     1561 ataatcatac ttatcagttg gataatcgat atcaggtagt ccaatccaac tgacaatcag
     1621 agcacgacca tcaggagtat tgaatccttg agtcgcatac gcttcaaatc cataatcaag
     1681 attttgaatt tctgaagcgc ccacaagttt tactttttct atgtcaagcg attgacagat
     1741 cttaaaagta ttcggataga tattcccata atttagctca gatttatcca acccttgagg
     1801 acaataaatg agaacaggtt ttttgtctac aaatactaga tttggacact caatcatgta
     1861 ttctgaacca gtgccgccaa aatcaaggtt gccaacttcc tcccatgttt ccacgttatt
     1921 gtcatccgct ttgtaaagtt tgataaagcc tgatttttct aaattttgtg ctccaacaat
     1981 cgcatacaat tgtccttcat aattaaacat ttgtggatca cgaaaatgat cagttacatc
     2041 agcaggttta tgaataagaa cattctcacc tttcgtaatg ttgtagtctt tatccatcca
     2101 tgctccgatt tgaagtgtat cacgttccca attttcattt cgaacgtttc ctgtatagag
     2161 taagaataga ttcccctcta tttcatatgc agaaccggaa taagcgccgt ggctatctcg
     2221 ttttgtgtct ggaagaagtt ctgctcctgt tacatgaaaa tgaactaaat catctgattc
     2281 attgtgtacc cattctttca acccatgagc agctccaaat ggccaatttt gataaaaaag
     2341 cgtatatttt ccgttaaaaa aagaaaaacc attaggatcg tttaacaatc ctgttttagg
     2401 ttcgatatga tagctagcac gccaaggtga tcgttttaca ttatccttaa ttttatccat
     2461 ctcatcttta gtccaatcag aataagcacg atatcgtatt tctcgaggta agttcatatt
     2521 ttactccttc aatttatctt tttacaacta gtatggtaaa cgttttccgt tgaaaaatca
     2581 atcatttcat ttaaaaaaat aaactatatg acaaacgttt gacatatttt taagatatgc
     2641 taaaataaat ttgcatttca gtgaaccgtt ttcacgtaaa aataaaaaag gagtgattcg
     2701 aatgaattac agtaaagtag cttccgacgt aatacaagcg gtcggaaaag ataaccttat
     2761 tgcagctgca cactgcgcta cacgtcttcg tctcgtacta aaagatgaca cgaaagtgaa
     2821 tcaaaaagct ttagacgaaa atccagatgt gaaaggcaca tttaaaattg atggacaata
     2881 tcaagtgatt atcggtgcag gtgatgttaa tttcgtttat gacgaactta tcaaaaaaac
     2941 tggactttcg gaaatttcaa cagatgattt aaaacaaatt gctagtaaag ataaaaaatt
     3001 caaccccatt atggcattaa ttaaattatt atcagatatt tttgttccaa ttatacctgc
     3061 ccttgttgca ggtggtttat taatggcatt acaaaacttt ttaacagctg ctggactttt
     3121 tggaccaaaa tcaattgaag aaatgtatcc tgcgattgaa ggtatttcct caatgatcca
     3181 actgatgtcc gctgcaccat tcatgttctt accaatttta gtaggtattt cagctgctaa
     3241 acgttttgga gcaaaccaat ttcttggtgc tgcaattggt atgatcatga caacaccaga
     3301 tcttggtggc tcaactgaat attggaatat ttttggttat catgtttcac aaacaaacta
     3361 tgcttatcaa gttattccag ttttagcagc tgtttggata ttatctatat tagaaaaatt
     3421 ctttcataaa aaattgccat catctattga ctttacattc acgccattat tatcagtaat
     3481 gattactgga tttttgactt tcacagttat tggccctgta atgttattgt tgtctaatgg
     3541 aattacagac attattgttt ggctatataa cactactgga tttattggaa tgggtatttt
     3601 tggaggaaca tattcactta tagtcatgac tggtttacat caatcatttc cagctattga
     3661 aactcaatta ctttcagcgt ggacaaatgg tatcggacat ggagacttta tctttgtagt
     3721 tgcatctatg gcaaacgttg ctcaaggtgc tgcaacattt gccatttggt tcttaactaa
     3781 aaattctaag acaaaaagtc ttgcttcctc tgcgggtgtc tcagcacttc ttggaattac
     3841 agagcctgcc ttgttcggag ttaacttaaa ataccgcttt ccattctttt gtgctttaat
     3901 tggttcagga attgctacag cagtagcagg attacttaaa gtagttgctg tttcacttgg
     3961 ttcagcaggt ttcttgggat ttctttcaat taatgcaaca tctattccat tttacgtatt
     4021 gtgtgaattg attagctttg tagttgcttt tgcaattact tacttctacg gtaaaacaaa
     4081 agctgctggt atctttaaag cagaagaaat tgctgaacaa acaccagtta attcttcaaa
     4141 agtagcaaca acagatccaa tatctactga tgttgaatca acacaaacaa aagtttcatc
     4201 agaaacaatt gttagtccca tttctggtaa agtgattagt ctttcttcag taaatgatcc
     4261 agtcttttca tcagaagcta tgggaaaagg gattgccatt aaaccaaatg gaaacacggt
     4321 ccattctcca gtagatggta ctattcaact cacgtttgaa actggacatg cttatggtat
     4381 taaatcaaat aatggtgctg aagttctaat tcatattggt attgatactg tatcaatggg
     4441 aggaaaagga tttacacaaa aagttactac taatcaaaaa gttagaaaag gcgatattct
     4501 tggagcattt gaccgttcaa tgattgagaa agaaggtctt gatgatacaa caatgattat
     4561 tgtgaccaat acttcggatt attcagaagt aaatatagta gctgaaaata cagtcactga
     4621 gggtactgat ttacttgaaa tcaaataaat atatttccct gagtaaagat ggaattttct
     4681 ttcca
//
LOCUS       DAXWLF010000177         4654 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXWLF010000177 DAXWLF010000000
VERSION     DAXWLF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.180"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..180
                     /locus_tag="ACGE4U_002431"
     CDS             <1..180
                     /locus_tag="ACGE4U_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394535.1"
                     /translation="FFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEFENAL
                     VICMTCTDEVFRKHF"
     gene            complement(554..1366)
                     /locus_tag="ACGE4U_002432"
     CDS             complement(554..1366)
                     /locus_tag="ACGE4U_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394536.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1529..1636
                     /locus_tag="ACGE4U_002433"
     CDS             1529..1636
                     /locus_tag="ACGE4U_002433"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394537.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1694..2761
                     /locus_tag="ACGE4U_002434"
     CDS             1694..2761
                     /locus_tag="ACGE4U_002434"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFF8394538.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            2903..3106
                     /locus_tag="ACGE4U_002435"
     CDS             2903..3106
                     /locus_tag="ACGE4U_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFF8394539.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            3166..4368
                     /locus_tag="ACGE4U_002436"
     CDS             3166..4368
                     /locus_tag="ACGE4U_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8394540.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         1554 a          744 c          971 g         1385 t
ORIGIN      
        1 tttttcattg caataattgc aacaaagaaa tttcatcggg ggaagaggtt tggacaaaaa
       61 tggaaatttc cgccaaaagt aactgctcct caactgaaat caattaaaga actagaattt
      121 gagaatgcac tggtaatttg tatgacatgt actgatgaag tatttcgaaa acatttttaa
      181 aggttgagat gtatatagat ttaaccaatc atttatctct ctcggaaaag gaatattaac
      241 taaccagcgg gttatcaaaa ataggtagga aaactgtact taatggctta tttcgcgctg
      301 attatgagaa tatcagtctg tttgattaat ttttagggtt caaaatacag agacaaactc
      361 caggtcgggt ttcgccacaa gcgaattacc cgatctggag ttttgtctat tgcaggaagg
      421 cgtagccgga ctgcaggaat aaagcccccg gttctaacag ggggctaaca aagaacagga
      481 aactgtgaca actacccatt ggggagtgtc ctagcagtat tttctttgtc acgtgcttat
      541 caattttgtc acctcatttt gatgatggat acgctgcatt taacaggcgt tctttctcga
      601 cttccatcac gaatggtaga taaagatatt gctgttctag tagatattga ttgtcctctg
      661 tgtactgata agaagcggtt tcttctgaat agaccaacgg ggcaattcgc acatcgctgg
      721 tcaattgatt aattgagtat agaaggatgg ctttctcacc tagttccttg ggtaaatgtt
      781 tcccaaaata ggaataggat ttttgatttg ctaaatagac gagtatgcga tccacactaa
      841 agttggagaa ggtaaagggc atcatgtgca gaatatgatt ttgattatca tgttgcaaat
      901 agagctgggc ttccttacca aaattagttg aaccaagata aagctgcttt ttttcatcga
      961 tacattggaa agtaaactgg tcttgttcag ttggtggaat gagatttgta ataaaaaatt
     1021 caatcattcg atccatttct ttttcactaa caaggtcagc gtgtttgatt tcgtctacta
     1081 tgagaccttc aatcttagga tcaagcttac ctgccttttt ttggagcatt tctaaaatgt
     1141 attgattctc ttgatcacca tacaaaagat attcattggt gacatgacct atctctgcga
     1201 tttggtgaag ggtttcggtt tttggaaggt tgatcccacg ttcccagtta ttgactgtac
     1261 ttcgcggtaa atcaccaatt agtttgccaa atttctccat actcaacttt aattcttggc
     1321 gaatctgccg aattcgattg ccgactgctt gcttgtctat ctccatgaaa actcctcctc
     1381 aggataagtt cttaaattta gtatacgtgt gtacttaaaa aaagtcaaaa catattgact
     1441 taaaataaat acaggcgtac aataaaaatg tacttaaaat aagttcaata ggttctttga
     1501 caagtaaata gcagaaggag gtttgtcgat gaaccaagtt aatcaacatg atctcggtga
     1561 agccattcga gtttctcgag aagaaagggg gtggacacaa cgatatttag cagaaaaagt
     1621 ggggatttcg agatagttat tatcgaaagt agaaaaagga acgagacaat taagtgaaga
     1681 aaaattgaat cttgtgttgg atagtttgca agaagaggtc gttccagtta accgtgtttt
     1741 gattgattat ctaaccattc actttttctc gaatcaacat ttaaagctaa ttgaagagat
     1801 tattgaaatg cccattgaac gttttgagga actggattat gcgccaaagg gctatattgg
     1861 ccaatacgtg tggaatcaag taatcacgat tcggtattcc attgacgatg cggttaaagg
     1921 aactgtgatg gagttttcag gtcaaggctg taaacactta gcaagatggc taaaaacacg
     1981 aaaaacaaat tggcaacagt tttttcgtaa ggtattagat tatcagggga actttacacg
     2041 gattgatttt actttagatg attttgtggg gagtctcagc attccagaac ttaagcataa
     2101 ggtaatacta ggccatgtgt ggaccacttt tcaagtgagt gaatctcatg gcggtacgga
     2161 tatcataaac aatgaatcaa atggtgagac cttgtattta ggctcaaaga agagtcagtg
     2221 tcgtttttgc ttttatcaga aggaccatga acaacggaag cgacgaggaa ttcctttaga
     2281 agaggcaaaa gtaaagaatc gctttgaact acggtatcga aaagagaaag cgcaaagctt
     2341 ggcaaagatt atttcaaaaa cccatgattt aaccaaactg ttctttgagt tacttaatgg
     2401 agcaatttgt ttttatgacc gtgatccaaa tgatctaggt gcaaaagttg atgccaaatg
     2461 ggcagccttt attagcaatt atggcgcaat caccatttct ttagatacaa ttcctcaaag
     2521 ttttgagaaa actatgagtt ggttaattca tggtgtttca ccatcattag tttttattgc
     2581 acaggtcgat caaacgtttc attcttcctt gttgcctttg attattgaac aaggagaaat
     2641 gaatccaaga cagatcaaac ttctccaaaa tatgaaggca gacccggagt attatcagga
     2701 ggaggttgag tattatgaac gaaaattaca aagtatatca aatgagaaaa caagctacta
     2761 aaaaagcaaa gtactgctta cacaacaagt tgtgcaacgt actccgcctc gacaagacga
     2821 gtataacaca accatcgaat gagaaacaat agattataaa gaggattatt ttaaaaggag
     2881 taaagaatca ggaggtgaga gaatggaaac aaaatgtgat ttcatggtaa atcgtgcaat
     2941 tttgatagag atgggcttta aacctagtca agctgcacga atgattaaag agagcaaggc
     3001 atatcttgca aggattgaag gtattgattt ttataataat cgccaagttg gtgtggtgcc
     3061 atcccgagta attgaacatc tttttcacat tcaagttgcg gaataaacag tacaagcgcg
     3121 gtactattaa agtagctttt ctcaagtaca gggaggaatg gaacgatgcc aaaaattaaa
     3181 aatatttacc aagataaaaa gacaaaaaaa tggttcttcc gagcgtatct aggtatagat
     3241 gaaaatggaa gaaaggttca gaagactaaa cgaggttatg cttcacaaaa agatgccaaa
     3301 attgcttatg acaagtacat ggaaacacat gattttaaca agacaattcc taatcaactg
     3361 tgttctacca atcaaatgac tttcgaagag ttttacaagg ataggtttgt aaaatggtac
     3421 gagagacagg tcaaaagtca aacatatgag aatgcgcaat tcattttcga aaagcgtatg
     3481 cagtttttct atcattttcg tgttcgagat attacaagtc acgatattga ggattggtta
     3541 tttgaactaa gccagacaga gtcacgtaat tcacggaaaa aagttaatgg cgaaacgtta
     3601 tcaaaatctt atatcaatcg gattcgaggg cacctaaaaa ttgtattgga tcgggctgtt
     3661 aaagaggggg tgattgctaa aaatcccgtt gaagatgttt cttcgcttcc ttctgagaat
     3721 aagaaagttg agttttggga acacagtgag tttcaaaaag tgatgaatga attgaaggat
     3781 gataagattc aaacccacca tcggaagatt gtgtatgaaa tgctctttta tactggcatt
     3841 cggattggag aacttgaagc tttgtcttgg tcgaatgtag attttgagaa aaacacgata
     3901 acgattgaga agacattgat ttataataca aaagatgatt ggtacttttc gactcccaaa
     3961 actaaatgtg cttatcgaac gattgggttg gggaagaagc tttccttaaa actcagacag
     4021 tggtatgact tgcaaaaacg tattggaaat tttgaatatg ttgctcagtt ggacggaaca
     4081 tttacgccac cttattcttt tgcgaactgg ttaaaagaag ctgctaaaaa agcgggtgtc
     4141 aaaccgatta agcttcacgc gctacgacat tctcatgttg catttctaat tgagcagaat
     4201 attcaaccgc tatctattca agaacgattg ggccatgcga atattcaaat cactttagga
     4261 acttacggtc acttatatgc aaagtcagat caacaagtgg taagtgcgat tgataatgtt
     4321 caagagggcg ctattctttc aaaaaatgac aatttattac cagtctgagt tttccacggt
     4381 tctaccataa gacaaataaa tgcttttgca ccagtgtttt cagtcgaatt gctcctgttc
     4441 aagatgtatc ggaaatagcg agaaaataag caaatttgca taactataat aaaaaggact
     4501 tagttttgat gggaaatttt tcaaaattaa gtcctttttt atgtggttga aacgggtatg
     4561 atcattttaa ggaagtgatc taagatgaaa ggacgactga acaattatca tattagaaaa
     4621 gctttattgt attccacaaa caaattacta ggaa
//
LOCUS       DAXWLF010000178         4645 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXWLF010000178 DAXWLF010000000
VERSION     DAXWLF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.181"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="ACGE4U_002437"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="ACGE4U_002437"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="ACGE4U_002438"
     CDS             complement(227..697)
                     /locus_tag="ACGE4U_002438"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFF8394541.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="ACGE4U_002439"
     CDS             complement(856..1314)
                     /locus_tag="ACGE4U_002439"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFF8394542.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2630)
                     /locus_tag="ACGE4U_002440"
     CDS             complement(1485..2630)
                     /locus_tag="ACGE4U_002440"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFF8394543.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2643..3560)
                     /locus_tag="ACGE4U_002441"
     CDS             complement(2643..3560)
                     /locus_tag="ACGE4U_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFF8394544.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3988..4551
                     /gene="efp"
                     /locus_tag="ACGE4U_002442"
     CDS             3988..4551
                     /gene="efp"
                     /locus_tag="ACGE4U_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFF8394545.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1430 a          868 c          896 g         1451 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct
     2041 agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa
     2101 taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg
     2161 gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt
     2221 gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc
     2281 gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag
     2341 acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc
     2401 ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt
     2461 tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg
     2521 ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactgac agctcctgtt
     2581 gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc
     2641 tcctataagt aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg
     2701 acacgatgac caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca
     2761 ctcgaacttc caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct
     2821 tctacatcag agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg
     2881 aattctacac cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag
     2941 ccctctggtt ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa
     3001 gttccagcaa aggttccacc actcccaatt cctgctacaa aactatggat attttctttg
     3061 atttcatgaa agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt
     3121 tcaaattgaa gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg
     3181 atggcaccga tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc
     3241 atcaattgtt gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg
     3301 tatgtgagtg cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc
     3361 gtcgtttttt cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat
     3421 ctatctttga tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacct
     3481 ttaggcatag aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct
     3541 gtaattgtcg agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac
     3601 tattaaatca gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac
     3661 ctggtactca ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg
     3721 caccatacaa cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc
     3781 aagtaaaatt tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa
     3841 aaaatatttc gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc
     3901 ttaaaagata gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta
     3961 tgataaagaa aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg
     4021 actttcgtac aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt
     4081 aaaggaaata cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac
     4141 acaacgttca gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa
     4201 tatttgtaca cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat
     4261 gaaattcctg tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta
     4321 aaaatccaat ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta
     4381 cgtgttgtgg aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca
     4441 gcgacaatgg aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg
     4501 ttggaaatca atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac
     4561 ggattgataa gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc
     4621 ctttcaagag gttttttgtg gaatc
//
LOCUS       DAXWLF010000179         4638 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXWLF010000179 DAXWLF010000000
VERSION     DAXWLF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.182"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(213..443)
                     /locus_tag="ACGE4U_002443"
     CDS             complement(213..443)
                     /locus_tag="ACGE4U_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFF8394546.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(673..1491)
                     /locus_tag="ACGE4U_002444"
     CDS             complement(673..1491)
                     /locus_tag="ACGE4U_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8394547.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="ACGE4U_002445"
     CDS             1652..2341
                     /locus_tag="ACGE4U_002445"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFF8394548.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="ACGE4U_002446"
     CDS             2355..3857
                     /locus_tag="ACGE4U_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8394549.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..4346
                     /locus_tag="ACGE4U_002447"
     CDS             3870..4346
                     /locus_tag="ACGE4U_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFF8394550.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4505..>4638)
                     /locus_tag="ACGE4U_002448"
     CDS             complement(4505..>4638)
                     /locus_tag="ACGE4U_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8394551.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         1546 a          666 c          816 g         1610 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1621 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4321 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4381 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4441 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttatga
     4501 tttgtcaaat taagctagaa taaaatgctt cttgtacata gctcaaaaat atttcgattg
     4561 gtgttctgta attcaatgat tttcttggaa tatgattacg ttgattgctg acactggaaa
     4621 taaatgtctg attcactt
//
LOCUS       DAXWLF010000180         4611 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXWLF010000180 DAXWLF010000000
VERSION     DAXWLF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.183"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(105..629)
                     /locus_tag="ACGE4U_002449"
     CDS             complement(105..629)
                     /locus_tag="ACGE4U_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFF8394552.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACGE4U_002450"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACGE4U_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFF8394553.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACGE4U_002451"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACGE4U_002451"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFF8394554.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACGE4U_002452"
     CDS             complement(2351..3031)
                     /locus_tag="ACGE4U_002452"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFF8394555.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACGE4U_002453"
     CDS             3157..4026
                     /locus_tag="ACGE4U_002453"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8394556.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACGE4U_002454"
     CDS             4052..4567
                     /locus_tag="ACGE4U_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFF8394557.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
BASE COUNT         1309 a          985 c          851 g         1466 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc t
//
LOCUS       DAXWLF010000181         4600 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXWLF010000181 DAXWLF010000000
VERSION     DAXWLF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.184"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            33..1094
                     /locus_tag="ACGE4U_002455"
     CDS             33..1094
                     /locus_tag="ACGE4U_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFF8394558.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1097..1993
                     /gene="murQ"
                     /locus_tag="ACGE4U_002456"
     CDS             1097..1993
                     /gene="murQ"
                     /locus_tag="ACGE4U_002456"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFF8394559.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2007..3989
                     /locus_tag="ACGE4U_002457"
     CDS             2007..3989
                     /locus_tag="ACGE4U_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFF8394560.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ACGE4U_002458"
     CDS             complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ACGE4U_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFF8394561.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         1488 a          818 c         1043 g         1251 t
ORIGIN      
        1 aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg
       61 attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg
      121 gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga
      181 tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag
      241 aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg
      301 taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc
      361 aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat gaattgaaag
      421 aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca cgtcccgaaa
      481 cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc
      541 ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat caaggattgc
      601 caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat ctattagcaa
      661 gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt caggagcaat
      721 ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta gatgaagaat
      781 tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga gatgtcatac
      841 gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat acagctacgc
      901 gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg ggtgagatcc
      961 aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct aaagttatca
     1021 aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa tttataagaa
     1081 aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa
     1141 ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac taatgaacaa
     1201 agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg aaccagtgat
     1261 tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg gagccggaac
     1321 aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc
     1381 ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag ctgtcgaagg
     1441 ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt tgacaaaaaa
     1501 tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg gtggattaga
     1561 ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac aagccgaaat
     1621 cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt tgacaggctc
     1681 tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt ctactatcag
     1741 tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac caacaaatga
     1801 aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg attacgaaac
     1861 agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg tcatgatttt
     1921 gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg gatttgtaaa
     1981 agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg gaagaactgg
     2041 caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt gtccactgca
     2101 tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct gaattaaaag
     2161 cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc atcggacctg
     2221 ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa
     2281 aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa gaccgtgtaa
     2341 acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca
     2401 aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca tttgtcggta
     2461 ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc ggaacacttg
     2521 atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac gggatgttta
     2581 gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc
     2641 ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga
     2701 aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct
     2761 tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg
     2821 tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt gaaattttct
     2881 tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac
     2941 ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta
     3001 tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc
     3061 gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg
     3121 cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac
     3181 cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac
     3241 gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg
     3301 gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg
     3361 acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg
     3421 ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa
     3481 cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga
     3541 ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag
     3601 tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct
     3661 attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca
     3721 caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg
     3781 gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag
     3841 cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg
     3901 taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa
     3961 aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa
     4021 actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg
     4081 ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa
     4141 gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt
     4201 tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg
     4261 catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata
     4321 agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat
     4381 ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc
     4441 tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata aaatgttcat
     4501 ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt ttggtggtgt
     4561 aatttggaaa gggactaggc gctttgcttg tataatgaga
//
LOCUS       DAXWLF010000182         4506 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXWLF010000182 DAXWLF010000000
VERSION     DAXWLF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.185"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..315)
                     /locus_tag="ACGE4U_002459"
     CDS             complement(<1..315)
                     /locus_tag="ACGE4U_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8394562.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFP"
     gene            547..822
                     /locus_tag="ACGE4U_002460"
     CDS             547..822
                     /locus_tag="ACGE4U_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394563.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            920..2917
                     /locus_tag="ACGE4U_002461"
     CDS             920..2917
                     /locus_tag="ACGE4U_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394564.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(2993..3328)
                     /locus_tag="ACGE4U_002462"
     CDS             complement(2993..3328)
                     /locus_tag="ACGE4U_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8394565.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            3455..3670
                     /locus_tag="ACGE4U_002463"
     CDS             3455..3670
                     /locus_tag="ACGE4U_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8394566.1"
                     /translation="MKKVNNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            3707..4069
                     /locus_tag="ACGE4U_002464"
     CDS             3707..4069
                     /locus_tag="ACGE4U_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFF8394567.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            4115..4441
                     /locus_tag="ACGE4U_002465"
     CDS             4115..4441
                     /locus_tag="ACGE4U_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394568.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         1605 a          603 c          867 g         1431 t
ORIGIN      
        1 tggaaatgtt ataccatcta gtacaatgct atctctataa agaataattc ccaaaatata
       61 ttcttctaca ttgccacaaa ctaactcctc tatactcata tttcccaaat cagcaattcg
      121 ggataatcgc tctggtattg gtaaataggt tcccttctcc cattcgtaga tagttttttt
      181 gtcaattaat tgaccgttgt taatcataag accaaatgct ggttgtgtta attttttact
      241 ctttcttata gcttttattt ttgttccgag tatctttttt cgttctaaat aatttattgg
      301 tggacgtttt atcatattga ccacacctcc taaaattagt atacctcaaa aaaggggtgt
      361 tgtaaataaa ttttacaaag ctgttgacac ccctttttta tcgaggtatt atgtgtatgt
      421 cgatgaactg gtaatcattt tttgtttgat taatcggtta ataacaaaat acttttattt
      481 tgagcaaaaa atacgaagat aattcttaga aaatttgttc aagcaatcac gaaaggaggg
      541 ggaaatatgt ctctacaaat cattgatagc attgtttcta tacaagttga acaacaagtg
      601 aaagagataa ttacagtgct aaagcctatg ctagaagaca ttatttctgg agtcactcta
      661 gagtatatgg actatcagca ggcaagtgaa tatctaggtg tatcagtagg aacgattaga
      721 aaatatgttt ctcaatacgg gttacctgtt ataaaaattg atacggtcat tagatttaaa
      781 aaatctgata ttgatagttt catggaacgg tataaaggtt gagtaaacaa ataagactaa
      841 aacatatagt aataaagagt ttgtttttat tatctgtatg ttttagtctt taacagaatt
      901 ttataaggag gaaacacaaa tggaaacatc aatgattaat attccaattt atgtagatca
      961 agaaaaatta ttagagaagc cagttacaaa aaaagaaaaa actgatgtaa gagataggtg
     1021 tttagataac tatagagaag ttacaagaga tgagtttgta gagatactaa attcatcaca
     1081 atcatttatt ccaagcaaga caaagaataa aggcaatgac aaggagagtt ttgtggaaac
     1141 cagagtaatt attctggatg tcgataacac tgttaaagat gagaagggga aggtagtaga
     1201 tttgagtaaa gatgatagta gatacttaag catagaaaag gttttatcta tcaatttagt
     1261 tcataattct gcgtttgcga tacaaaaaag tattcggtac tctgaaaatc tagaaaaata
     1321 caagattgtc tttctattaa aagaagcaat tactgactat ggtaaaatgg ttgctgttta
     1381 cgaatacttg aaaaagaaca tccccgggtg tgatgacaat gtaaatgctt cgaatagaat
     1441 gttttttgga ggatataagt cagatgcagt gataataatt aatgaagaca acatgttaga
     1501 tattactgag cttccgattg attttaccat gacatgttct aaaaattgtc aaagtgattc
     1561 agggagagag tatgaactag tcaatgaaac cgattttgtt aagttgataa aaaatgacga
     1621 caaagaagaa atgaaacagt ggtttaaaga ctgcctgttt gattcctccg acatgtcttt
     1681 taatgagatt tatgaaagat tgttaaccat tgatatgaat aaattactta agtcaagtaa
     1741 aaacttaaga tgtctgtttc acaacgatca taatccctct gctagtattt ttaccagtaa
     1801 aactgggcat tccatttttt attgtcattc cagtaattgt gatatcagtg aaaattttat
     1861 tggagttgtg atgctaattt taaagaaaga cagtcgggta gaaacattta attggctatt
     1921 agagaaccta gacttgtatc ctacacattt tcgaaaattg aaagaggaat caaaagtact
     1981 ttttgacact cttgaatcaa tgaaagacaa acttttcagg acaataagga attatctaga
     2041 ggaaatgcga cgtgtctatg atgtcttgct ttctgatgtt aatttttttg atgatagtaa
     2101 ggaaagtgtg acttgtattt tgtcggggga acaattagct aaaaaaatga gttcttacta
     2161 tggtaaaact tatgacatag ataaatgtaa taagttacta tcacttatga cttttatagg
     2221 attgataaaa aaattagatg atgaagatgt tcctttgaag atgctcgaat acctaaatag
     2281 gttaaaggaa gagaaagcgt taggaaactt taatcataag agaagcaatg tctatcgatt
     2341 agacgttttt aatgcaactt ctgtgattca aaaattgaat gaagatatcc ttccacttct
     2401 ggaaaaaatt cattttacat atcaatattt ttcttatgaa tgggttaaga tatgttttaa
     2461 cgaaaaagag gctaaaagag tatttcctca aaattcccga tcagaactca gcaaggaaaa
     2521 acaattgatt ttagatgaag cacatgaagt tttacagggc ttatcgtggc aagggacata
     2581 tctaatgaca gagaaagaac ttattaacac tgtttgtgaa cgcctacgta atgttgggga
     2641 taacagcgtg aaaaatgctt taataaaaaa cagaggctac tttgttaaac aaggattcac
     2701 tcttgaacga gcttctaagg atattaagaa actatacaat gtgaaagcaa gtagtagttt
     2761 tttaatatat ttagcgaaag gggcagggat agatgaaaca ataaaagaat tgatattatc
     2821 aggagctggg atagttcctc tgtatcaatt tacaagtgta acaagagatg atggacggag
     2881 agtaaggaag agaaaagacc caattgtatt aaactagaca ggttatccat agctcttaat
     2941 cttatttcat ctcaatacac ccaccgttaa agttcggtgg gttatttatt tttcaatctt
     3001 tcaatagctg attgcgttca ttttcttgcc tttttaatag cttcatatag tcaattgttc
     3061 gaactgcatc ttcagtgcta agagaacgat agagagccaa aaactcttcc tcataatcta
     3121 cgacaacatt gtctagaaaa tggcttacag gggttcctag ggctctagaa agagctgtta
     3181 agctatcgag gttaggctct gttacagaat tttcccatct tgctactgcc tgttgtgtag
     3241 agaagatttt ttcagctaat tctttttgtg tccacccgag agcttttcgc ttttctctaa
     3301 tgacctttgc atattgtttc tgtttcatta atacaccccc atctcagtat agtacaacaa
     3361 gttgttgtat ttgtaatggt atttgaagtt ttttatattt tatgagttga aatacatcat
     3421 attgttgtat ataataaaac aggaggggag aatcatgaaa aaagtaaata atctcagccg
     3481 atacagaaaa gaaaaaggac tttctcaaac ggagttagca aaaaaaatga atgttacaca
     3541 gcaatgtgta agttcgtggc aaacagggag aacgattcct aaaccctatc aaatgaagat
     3601 gctttctgag atattaagtg taccaatgaa tgagctgttt tctgatgtat ttaataaagt
     3661 aaacagttaa ctaataagca attgaatgat tttaggagga gaagacatgt attgtagaaa
     3721 atgtggcaaa gaaataacag atgaaagtaa gtattgccag ttttgtggag cactacaaga
     3781 aacagatgag acaaccggaa cagacacagt aaagaaaagt actgagcaga caattaatga
     3841 aatgaaggaa caaatcgtac atggacaaga gacagggaag ctgtatttga ttattggttg
     3901 gatttctatg gtagtatcgt tagcatttat tcctgttgta tttggtgctg ttgctgttat
     3961 tatgggctac ttatatcgag aaaaagatga gaaaatgggt acgattttga tgatagcagg
     4021 ggtagctgga gcaatctttg gtgtactttt agggatgtca atgtactgat aagagggata
     4081 agaacaaatt attttggaga taggagataa aaagttgaat aaagtaaata tgaacaagaa
     4141 gtttgtcatt ataggtattg ttgtcttggc tattataggt attatttttt caaaaatgac
     4201 aggtacatct ggattagaga agtcatcaaa accattggta acacagattt tacaagaaca
     4261 gtacaactta aatcgatcat gtgatgatgt gacgattact catgaaaatg gagataacac
     4321 ataccgggct aaagctatat tagacaatgg aagtgctatc aacataaata ttgagtacta
     4381 tccaaagaaa gaccgtatat atgtggagat accatatgct gaggtgttaa tgttaaattg
     4441 atttaaatgt attatgcata ccgtccaaac aggaactccc aaaggtgtaa attcactagg
     4501 gagttt
//
LOCUS       DAXWLF010000183         4471 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXWLF010000183 DAXWLF010000000
VERSION     DAXWLF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.186"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(16..1239)
                     /locus_tag="ACGE4U_002466"
     CDS             complement(16..1239)
                     /locus_tag="ACGE4U_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8394569.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(1270..2109)
                     /locus_tag="ACGE4U_002467"
     CDS             complement(1270..2109)
                     /locus_tag="ACGE4U_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8394570.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(2126..2806)
                     /locus_tag="ACGE4U_002468"
     CDS             complement(2126..2806)
                     /locus_tag="ACGE4U_002468"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFF8394571.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(2808..3881)
                     /locus_tag="ACGE4U_002469"
     CDS             complement(2808..3881)
                     /locus_tag="ACGE4U_002469"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFF8394572.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(3937..4175)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(4218..4451)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1219 a          994 c          770 g         1488 t
ORIGIN      
        1 caagtttatt ttttactaga aagtagcgcc ctcttgtttt tttcttgcta ctatttcttg
       61 atgttcttgc ttgattcttt ctaatagctt tggctctttc aacagatcaa gagctgtatt
      121 ggcgagtagt tctgctgcaa tcgcaataga agcaaggcct ttgtcacttt tagcagcagc
      181 tttgaattct tctgtgtgtc cggcaatata tgtctcagag attgctaccg taggctggat
      241 tgttggtatg acttggctga catttcctac atcagaagaa ccgagacttg ttctttgttc
      301 cgtttctatt tcttcatctg gaacacctgc ttcttttgca tgctggaaga ataagtcatc
      361 aaaagaagga gtaacgatga catcatcaac tgcattttgg aacaatccaa actcaaaagt
      421 cgtgcccgta cttatcgcag cacctcgtac gatattttcc actttttcat atactttatc
      481 taatgtttgc cgatttcctg cacgtaaata gaaacgtcca gcagcgtaat ctggaatgac
      541 gttggcagct actcctccgt ctgtaatgat cccatggata ttgacatctt ttggcaattg
      601 caagcgtaga gcgttgattc cattaaacac ttggatcaat gcttccaaag catttactcc
      661 tttttcaggt gctgctgctg catgggaagc gacaccgtaa aatttgatgt cgactggatc
      721 attcgctaaa gattctgtcg ttttcccata acgaaaagct ggatggacgc ataacgctac
      781 atcgacatcc tcaaaaaaac cttctcgaac aaagctgcct ttagctgatc cgttctcgcc
      841 cccttcttcc cctggggtgc catatacacg gatttctcca ccagtttcat caatgaattg
      901 cttcactatg ctggcggcta atacggagta agtaccaaac aaattatgcc cgcatgcatg
      961 accaatacct ggtaaagcat cgaattctgc caaaaaggca attgtgggac cgggtttatt
     1021 ggaaatataa cgtgcatcaa aggctgttcg gtgtccagct acttcttttt ctacccaaaa
     1081 cctttctttt tctaattgtt taatcaacgc tgctgaggaa taaaactcat agttacttac
     1141 ttcaggatgt tcgtggatat ctagagcaag tgcttgatat tctggtaaac gatattggat
     1201 tagatccttc aattggtttg ttgagattgt tgttgtcata aaaaattcct cctatgctag
     1261 tcatttttct tattttctcc cgaatgtttc ccatgcaggc acgcttgatc cttttgatgt
     1321 tttctcgatt actttgactg tgtcttctgt ttgataagcg tctactattt tttgatacgt
     1381 tttattgttt tcatcttctt ttcttgcaac gatgatattc acataaggac gtgcatggtc
     1441 atccactggt tctaagaaga tggcatcaga agcaggatta aatccagcat ctacagctac
     1501 accgctattg atcactgaaa tgtctacatc tgacaaagca cgtgctgttt gggaagcatc
     1561 taattcagaa atgtctagat tttttctatt ctcggtaata tcagaaacag tgggcgcttg
     1621 tccttttgcc ttatccactt tgatcaatcc agcagtttgc aataacagaa gagctctgcc
     1681 gccatttgta acatcattag ggattgctac ttttgctccg tctttgatat cagctacatc
     1741 cttcaaagtg ttagaataaa ttcccagcgg tgcattgaca gtatttccga tcgatactaa
     1801 atcagttccg tgttctttat tgtaattgtc taaaaagaat tgatgttgga aagaattcaa
     1861 atcaatcgat ccatctgcta aagccacatt tggttgattg taatctgtaa atttcacgta
     1921 ttccaaatta atgttctcct ttttcaaacg gtctttcacg ttgtcccata catctgtatc
     1981 ttctccaata atccctagtt ttacgatagt ttcttttttg tcagttgtat ctgcttttcc
     2041 ttggctacac cctgccaacc aaataattgc tacaaatgta atgactacaa aacgaataat
     2101 ttttttcatt ttttctcctc cactttcaat gtgtaatttt tttaatcagt tgattactga
     2161 taaattgact taaaaaaact aagattagaa tcaataatgt agccacaatc gttacatctg
     2221 tttggaatcg attataccct ctcgatatag caagattccc taatccgcct gcacctactg
     2281 ctccagccat ggtagtcagt ccgatcacgt tgatgatagt caacgccgag acccgaatga
     2341 taccaggcaa accttctaac aaatacacac gtaaaatgat accgattgga ctggttccca
     2401 tcgcttctgc cgcttcgatc accccaggat caacttctag cagtgcgttc tcgatttgac
     2461 gagcaaaaaa cggaacaact ccaaaaatca atggcactaa agctgctttc gcaccaatcg
     2521 tcgttccaac taagaaacga gtagtcaatg ctaaaagaga aagcaagata atgaaaggga
     2581 tggaacgaat gatattgatc agcttatcaa gtatttcaaa tagcagacgg ttagctagta
     2641 atccattagg gcgtgtcact actaacgtca cacctaagat gattccaatc atcccgccaa
     2701 tgaatgctgt ccaaaaagac atatatagtg tttgcttcgt cgcttcgaca aattcatccg
     2761 gaatcaaata aacattgggc aaatatgttt taaagaaatc agtcatttta tcttcctcct
     2821 tcgatcactt tgatacgact atctaaatcg tttttttgta aaatatttat ttttacacca
     2881 gatttgatca aataatccaa tgcttttttc cgctgtttgg tttctccagc taaagtaacg
     2941 atcaaactcc ctaacggcgt ttcttgtaat aactctacat tgccatataa aatattcgct
     3001 gttacttgat atttgctgta cagatgagcg attagaggtt cgttcgtctg atttccaata
     3061 taacttaatt ccaccagcca tttattggcg atctgatcgg caaaagcaga atgggcaatg
     3121 atcgtttcta atgcttggtc aagatgagta gctgttcgaa taaaatcttt ggtcaattct
     3181 tcttcaggat tgctgaagat ctggatgctg ctaccttttt caacgattct tccatcttcc
     3241 ataaccgcta ccttgttgca gatttctttg acaacttgca tctcatgagt gatcaggacg
     3301 attgttaaac ctaactggcg attcaatttt tttaacaatg caaggatctg aagggtcgtt
     3361 ttcggatcaa gtgcactggt tgcttcatca cataataata ccttaggttc attcgccaaa
     3421 gctcgtgcga ttgctactcg ttgtttttgt ccccccgaca gttgtttagg ataagcagat
     3481 gctttttctt ccaaaccgac aagttccagt agttcattca ctttttgacg acgttcctgt
     3541 ttcgattttc cagaatactt taaagaaaaa tcaacgttgt caaaaatagt tcggctttcc
     3601 attagattga aatgctgaaa gatcataccg atggcctttc tttcttttcg caattctttg
     3661 ggagagagct gactcagatt cgtctgattg acaatgactt ctcctgctgt tggtttctgc
     3721 agcaaattca tcacacgaac taacgtactt ttccctgcac ctgagtaacc gacgattcca
     3781 taaacatcgc ctttttcaat cgttaaatct acagaatcta ctgcctgtat ctctttttct
     3841 ttttgtttga aacatacaga aacatctcgc aattcaatca ttaaaattcc tccttatcta
     3901 aaaaaagcgt cctcaagaaa atgaaaatgt cattttcttg aggacgcttt tttgcgtgtt
     3961 accacctcat gttcaagctg tgttcacaca caactcctct tcaagtacga tcatactcta
     4021 gcaagatatc ggtcgcagcc gtcatcgcct tacatctatg ctcgggatga attgctcgat
     4081 ggccattttc caatagtcgt tccttgcttc ttctcagcta ccgaagctct ctgtcaaagg
     4141 ttcttctatc gtacttacca tttcaatgca aataatattc aaaaaaaaag tccttttact
     4201 aacataaact atgtcagtaa aaggacgacg tatcgcggta ccaccttttt tcggaagttt
     4261 ccttccctca agcccaacat ctcaaaaaga gagattggcg ttcgttttaa cggtcgaaca
     4321 gttccgaatt tgcctactta tcgacaaatt attctccatg gccattttca gcttttctta
     4381 cttgactctt ctcaccagcc agagcctctc tgcaaagttt ccaaacttac tttccttttc
     4441 aacgaaatcg atattttgtt gttgggatta a
//
LOCUS       DAXWLF010000184         4420 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXWLF010000184 DAXWLF010000000
VERSION     DAXWLF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.187"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            61..915
                     /locus_tag="ACGE4U_002470"
     CDS             61..915
                     /locus_tag="ACGE4U_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFF8394573.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1012..3261
                     /locus_tag="ACGE4U_002471"
     CDS             1012..3261
                     /locus_tag="ACGE4U_002471"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFF8394574.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3264..>4420
                     /locus_tag="ACGE4U_002472"
     CDS             3264..>4420
                     /locus_tag="ACGE4U_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8394575.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1557 a          625 c          807 g         1431 t
ORIGIN      
        1 atgtaataat ataaacataa taaaacaaaa attatccaaa tctaacatag gagggtggat
       61 atggaaaatt ctatatattt agtaacagaa cctaacactc agctgaaaga ttgttacttt
      121 aatttttgtg ggtattcaaa aacagaacct agacatagtt ttggaccagc tattcgagaa
      181 caatacttaa tacacatagt gctagaaggt gaaggctatt attcaattaa tgatcaaaaa
      241 tattttctga agaaaggcca aggatttgtt atcccaccag gagtatctac tttttatcaa
      301 gcagatgaaa aaaatccgtg gtcatatgta tggttgggtt taagtggcaa tcttgtagaa
      361 tactatctaa aagaaattgg tgttggaaag aatcatctta cctttaaagt cgaaaattta
      421 aattcattta aaggattaat ttttgaaact ttatcgtatg atcaggacaa tctagtcagt
      481 gaattgtcca gacaaaaaca agtttataaa tttttagaac tcctcttaaa ttcagctagt
      541 tatctgaacg aagggataat tactaaaaag atgaatcctt ttgttcgtca aacaattgaa
      601 ataatacgtg agaatgcgta tcaaaaatta tctgttaaag atattgctgt cagtctatcg
      661 atacacgaaa attatttatc acgattgttt aagaatgata tgggaatatc aataaaagag
      721 tatattagtg aaatcaaaat ttctattgca aacgatctgt tatcatcaac tgaatattca
      781 atccagcaaa tcagtgacaa gataggattc tcaacagtac aatctttttc taaagccttt
      841 aagaaagcaa atggtgtttc accgggagta tatcgaaaac gtagattagg tctaggaatc
      901 attaatggtg aataaactaa acatttgatt ttgataaata ttcgttactt taaacgctta
      961 ctatttatgt gagcgttttt tataatgcaa taaagaaacg gaggagagct tatgtataag
     1021 gataatgaaa tgaatattat ttatcgatca gaaaaccaac aatttcattt gtttaatcag
     1081 gagataagtt acatttttaa gatatcggaa gatggtaaat tattgcattt atattatggt
     1141 gagaacttac cagataatga ctatagtcat ctaattgaga tgcatcatcg gcctatgaca
     1201 acctatagaa atgaaaacga tttgctatat tcgttagagc acttaaaaca agaatttcct
     1261 gaatatggta caacagattt tagacatcct gctatttcat tactacagaa aaatggctct
     1321 cgcttatcag attttgtcta tcaagggtac gaagtggaag aaggaaaacc taaactagaa
     1381 ggtttacctg ctacctatgt tgataataat gatgaaagca aaacattgaa agtttatatt
     1441 ttagattcag ttacaaatgt tcaagtagaa ttattatata caatttatag agattatcct
     1501 gttattactc gtagcgcgcg agttatgaat aaaggaaaag atattcaaat aatcgagaat
     1561 attagcagtt tatcattgga tttacccgat gcaaattatg aatggcaaca attatctggt
     1621 gcttggggta gagaaagaac tattaagtcg cgtactttac aacaaggaat tcaatcaatc
     1681 gaatctactc gaggtatttc aagccatcag cataacccat ttgttacttt aaaaagaaaa
     1741 aatactgatg aaaatcaagg agaagctatt ggtgcagcac tagtttactc aggaaacttc
     1801 ttaattcaag cagaagtaga tacatgggat gttactagac tacaagttgg catcaatcca
     1861 tttggattta attggaaact caatccagga gaaatattta cagcaccaga agcgattctg
     1921 gtttattcaa gtaagggttt aaatgccatg agtcaaactt ttcatcaact atttagaaaa
     1981 cgcttagctc gtggtaagtg gcgagaaaaa gaccgtcctg tcttaattaa taattgggaa
     2041 gcaacatatt ttgattttga cgaagaaaag ttggtaaata ttgctaaaaa agcatttgat
     2101 gttggtattg aattatttgt attagatgat ggctggtttg gagaaagaga aaacgattgt
     2161 gcaggattag gagattggca tgtaaatcct aaaagactac ctagaggaat cggcagcctt
     2221 tcagaaaaaa ttcgtagttt tggtatgaaa tttggtttat ggtttgaacc tgaaatggtg
     2281 aataaggata gtgagttata tcgcacacat ccgaattgga ttatttctac acctaatcga
     2341 aaacctaatc atggtaggaa ccaatatgta ttaaattttg ggttagatga ggttgttgaa
     2401 aatattttcg atcaaatgtg taaaatcatt gatgaatcaa acttagatta cattaagtgg
     2461 gatatgaatc gtccattgac tgatgtattt gacagtcact tgtcagcaga tcaacaagga
     2521 gaagtattcc acagatatgt actaggcgta tatcgcttat atgaaaaact tataacaaaa
     2581 tatcctaaca tactatttga gtcttgttca tcaggtggtg ggcgctttga tcctggaatg
     2641 cttcactatg cacctcaagc ttgggctagt gatgattcag atgcaattga aaggttaaaa
     2701 attcaatatg gaactagtat gctgtatcct ctttcgtcta ttggtgctca tgtatcaatt
     2761 gttccaaatc atcaaacaaa ccgtataaca cctataaaaa ctagaggaaa tgtagctttt
     2821 tttggtgcat ttggatatga gcttgactta aatgaattaa gtgaaggcga tttgaggatt
     2881 gtaaaagaac aaattgcctt ttataaaaaa tatagaacca taattcataa tggtactttt
     2941 tatagactat tatcaccatt cgatgatgat aatcaaaccg cgtggatggt tgttagtcat
     3001 gataagaaaa cggctattgt tgctcattat aaaacattaa atgaggtaaa tgctccttac
     3061 cgccgactga aattaaaagg gttagataat gacggacttt ataatgtagg acaaaatagt
     3121 ttgcgatctg gaatggaatt gatgagagca ggactagtct gttcagatgc atctagtgga
     3181 caaataaatg atcagaatcg acgcccagaa acatttgatt ttgattccaa gatttgggtt
     3241 ataacacagg aggaaaagta gagatgaata accaaaaaag cttacttcaa aaaggagcaa
     3301 tcttaagtat ttcactaatg ttaacaagct ctcaagcaat taatggtgtt attccacaaa
     3361 tgaaacagtc tttggggata tcacaaagtc aaagtgaact tttaggaact gcaccaagta
     3421 taactgttat tttgtttatc ttattatcta gttactttgc caaaagaata ggaatgaaaa
     3481 agacaataat tttaggttta ttattagctg gtataggagg aattattcca gtatttacag
     3541 ctaccttcca aaccgttctt attagtcgag taatattagg tgccggttta ggagtatata
     3601 attcattggc tgtgacatat atcagctctt tatactctgg caatactaga gcaacgctat
     3661 taggtattag aaattcaatg gaagctttag gtcagacaat ccttattttt cttgctggag
     3721 ttttagtgaa cattaattgg acggcttcgt ttttagtcta tgcaattgcg tttcctgtag
     3781 ctctcttatt cgcgctaaaa gttcctgaaa ttagagatga aaatagtgac atacctgaaa
     3841 atcatgtaaa agaaaagatg aatccaatgg tttttgccct agttctattt gcaatcttac
     3901 tcgtaatgaa tagtattgct atttctgtac gatttgcatc aattgcaact gaaattaaag
     3961 gggtaaactt taatgctagt aactatttag ctctaatgcc tatcttagga atactagcag
     4021 gatttatgtt tggaccaata aataagtgga taggaaaagg aaccctatac ttgggaatta
     4081 tattctttat aatttcaaat ttgttaattg ctgtgtcaaa cggtaatatg acgttcctgc
     4141 taacgggttt atttttatca agtataacag gatcatggtg cttcccattt atctttagta
     4201 acttagataa agtcactaca aagaatacaa ttaattttgc tacttcattg atttttattg
     4261 gctgtaatat tggtaatttt atagctccta ttgctatgca attagcacaa tttctaacaa
     4321 gaagtacaaa tttgactgct ccattttttg tattcgcagg aatatttatt gtgatcctat
     4381 ttgtaacatt ttttgcgaca aataaaaaaa atactagagt
//
LOCUS       DAXWLF010000185         4402 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXWLF010000185 DAXWLF010000000
VERSION     DAXWLF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.188"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            78..638
                     /locus_tag="ACGE4U_002473"
     CDS             78..638
                     /locus_tag="ACGE4U_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8394576.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            767..1144
                     /locus_tag="ACGE4U_002474"
     CDS             767..1144
                     /locus_tag="ACGE4U_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFF8394577.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            1077..3149
                     /locus_tag="ACGE4U_002475"
     CDS             1077..3149
                     /locus_tag="ACGE4U_002475"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8394578.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(3360..4307)
                     /locus_tag="ACGE4U_002476"
     CDS             complement(3360..4307)
                     /locus_tag="ACGE4U_002476"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8394579.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
BASE COUNT         1566 a          842 c          890 g         1104 t
ORIGIN      
        1 aaagtctctt ttttctcttt ctttttcgtg gtaaactgaa agagtgaagg atttatttta
       61 tgagaggaaa catggaaatg aaaaaaagaa atctgtctca agagaaaatc atcgactgct
      121 ttcgagaact agcagaagag atggatgttc agcaaataac ctttcagcat ttagccaaac
      181 gcttggatat caaggctccc tcgctgtata accatttcaa aaatattcgt gatgtcaaaa
      241 cagcgctaac agcgaaactt ctgcaggaac ttaatgacca gctgcgacgg gctcttgtgg
      301 gaaaaagtgg tcaggaagca ctgcagatct atgcagaaac ttatcgatcc tttgcttttt
      361 caaatcaagc agtgtatgag ttactgatca gtgtaccgca taccaatgaa gctgttttat
      421 tagatggaat ccatgaaacg aatcagatca ttttgcagat atttgaggct tttcaattaa
      481 cgaaagaaga aaaagttcat cgaagcaggg aacttcgtag tatgatccac ggttatcttt
      541 ccttacgttt tttaggatac tttacaaaag aaccaacaat cgatccagaa gacagctatt
      601 tttggatgat caataacttt atcgctactt tgccttaaac aatcagattc atttgtaaca
      661 attttaagat gttcattaaa tgaccctttt ataagagaaa attcttgcaa ctttcttgaa
      721 aagtgactaa gatgagtcta gaaaagctga aagaaggagc acgagtatgt atcaagtaat
      781 taccatgttc ggggacaatg agccttggtg gttttttgaa gactgggaag aagatattga
      841 agaagaagaa acattcgagt ctttcgaaga agcacgacaa gcgtacgaaa aacaatggaa
      901 cgaaatccat caaaattatg aatatatcaa tgcaaaaagt aactttctca gcgccttttg
      961 gaatgacgga gatgagcgct ggtgtgaaga gtgtgacgat gatttacagc aatacaaggg
     1021 acttgcgttg ttgaaaaatc atcagcccat cacagtagaa agcagaaagg aattttatga
     1081 aacaactaat tctagcggaa aaaccaagcg ttgcgaaaga cctaagcaag gtgcttggtg
     1141 ctaatcaaaa gcacaaaaat tattatgaag gacccaaggt gatcgttact tgggcccttg
     1201 gtcatttatt aggattgaaa atgccggaag atctgaataa ggaatggcag agctggcaaa
     1261 tggacacgtt gccaatgatc cctaaaaaac taggaatcaa accacttcct aaaacaggac
     1321 atcaattaaa agcaatcaag cagctggctc agcgaaaaga tgtctcagaa gcagtcattg
     1381 cgacagatgc cggaagagaa ggggaacttg ttgctcgctg gatcttagag tatgtgcatt
     1441 tcaataaacc agtgaagcgg ttatggatct cttctcaaac agataaagca atcaaaactg
     1501 gattcaagca actaagacct gcgaaagaat atgatgcatt gtatgaatct gctcttgccc
     1561 gggcaaaagc ggactggctg gtaggattga atgtgaccag agccttaact gtgaagtacg
     1621 cggataattt atcagcaggc cgtgtgcaga caccgacact agcattagtg agagaccaag
     1681 aaaagaaaat cgagcagttc cgtccacaaa cgtatttcac gatcacgttg caagctggaa
     1741 aagaacaagc caaaatgctt caaaaaaatc aatttgcttt aaaaacacaa gaagaagcac
     1801 aaaaacttgt tcaattgatc agtacaagca aaggaactgt cacagaagtc caagaaaaat
     1861 taaaaacgga atctgcgcca ttgccttatg atttaacaga gatccaaaga gaagccaatc
     1921 aacgctatgg ctattctgca aagaaaacat taagtcttgt acaaagtttg tatgagaccc
     1981 ataagatcgt tacttatccg cgtacagaca gcaaatatct gacaaatgac atgaaaagta
     2041 cgatgaaaga acgattgcaa gcagtcagcg actttgctcc tgaggtcaag cagtatttga
     2101 aaaacggagc catcgtccaa cagacaaaag tattccaaga cagtaaagtg accgatcacc
     2161 atgcactttt gccaactgaa aacagagcac gttatgaaaa attaagtaat gaagagcgaa
     2221 aaatctatca aatgattgtc tctcgttttt taggactgtt tgctcaaccc cataaagtca
     2281 gccaaacaaa agttacggtt gaatttggta aagaacgatt catttttcgg caaaatcgtg
     2341 tcgtccaagc aggatggaaa ggcgaaactg aaactgagac ggaaacagtc aaatgggaaa
     2401 aaggaatgag aatcaatccc gatttcgcca tcaaaaaaga actatctgca ccgcctaaac
     2461 cattgtcaga agcaagcttg cttggtcaaa tggaaaaata cagtctggga accccagcta
     2521 cgcgtgcaga gattatcgag aaactaatca aatctgaact gatggaacgt acccctcaag
     2581 gattgcaagt atctcctaaa ggtaagcaat tattagtatt ggtaaatccc tctttagtaa
     2641 caccagattt gacagaaagc tgggaaaaag accttgaagc tattgctgca ggaaagaaac
     2701 aagcaggaac cttcttgaat ggaatcgaaa aagaaacaaa acgcttggtg aatgagatca
     2761 aatcaagcaa acaagaatat caagatttct cgattaccca aaagaaatgc ccagaatgtg
     2821 gggccaatct gcgcgagaaa aacacacgtg acggcaaaat ctatgtctgc accaattcag
     2881 attgcaacta ccgcagaaga aaagatccaa aagtctctaa tcatcgatgc ccgcaatgtc
     2941 accggaaaat ggaaatcatc gaaggtaaaa acggtgctta tttccgttgt aaatacgatg
     3001 gaacgacaga aaaattgctt gataaaaaag agcagaagaa gaaaatgacc aaacatgaag
     3061 aacgacgctt gctgaaaaaa tatgcacaag cagaagaacc agaagaaagc ccattagcag
     3121 cagcactaaa agcagcaatg aagaagtaat aagatatgaa agaggttgtg atagaagtgg
     3181 tcagcttcaa gaaataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaattt
     3241 cggcttattt ccgaagaagc tacttctgga acaccattta ttcggaaaaa aggactgtga
     3301 caagactttt gtcacagtcc tttttcattt aaaaaaatgt ttacttcatt tgacgtatat
     3361 tattggtgag gcttcaattc atttggcttc actttttctg tttctcttat gatataaaca
     3421 ggacgctgtt tcgtttctaa gaagattttc ccaatatatt tcccgatgat tccaagacat
     3481 aacaattgga ttccaccaat gaacaggaag atcgagacca tggaaggcca tcctgatgta
     3541 ggatcaccaa acaacaatgc ccgcaaaata atgaacaaaa ttgcaatacc agaaccgaca
     3601 caagacaaag cgccgataaa tgaagcaatc gttaacggtg catccgagaa gttcacgata
     3661 ccatcaatcg agtagttgaa taatttccag aaggaccaag atgtttcgcc agccacacgt
     3721 tcgcggtttt cataagaaat atattctgtt ttaaatccga cccagctaaa taatcctttt
     3781 gaaaatcggt tgtattctgt taattctagg acggcatcca ccatttgacg agtcatcaag
     3841 cggaaatcac gagcgccatc gaccatttct gtatcgctga ttttattgat caactggtag
     3901 aattttttcg caaagaaact gcgtatcgca ggctcgcctt cacgagtcga acgccgggtg
     3961 ccgacacaat cgatatcaga agtagtgatc cggttgatca tctcaggaag cagttcgggc
     4021 ggatcttgca gatcaacatc catcacggtc actaaatctc ctgtagaagc ttgcaatcca
     4081 gcgtacagag ccgcttcttt cccaaaattt ctggaaaatg agacaaagcg gacataagga
     4141 taagctgagg ctagcttctt gattgctgct aacgtaccgt cttttgatcc gtcgtcaata
     4201 aacaaatatt cgaactcatg ggcacctttg aatttttcaa cctcatcaaa aaaaagagga
     4261 acagtttttt cttcattaaa acatgggaca acaattgata ctttcatcgt ttctatcctt
     4321 tcaatcgtat gtttatatat ggtttacatt caaaatttaa taaaccttaa ataacacctt
     4381 taaaatttga ttttacgtaa aa
//
LOCUS       DAXWLF010000186         4386 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXWLF010000186 DAXWLF010000000
VERSION     DAXWLF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.189"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..356)
                     /locus_tag="ACGE4U_002477"
     CDS             complement(<1..356)
                     /locus_tag="ACGE4U_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394580.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(616..1164)
                     /locus_tag="ACGE4U_002478"
     CDS             complement(616..1164)
                     /locus_tag="ACGE4U_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394581.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1165..2022)
                     /locus_tag="ACGE4U_002479"
     CDS             complement(1165..2022)
                     /locus_tag="ACGE4U_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFF8394582.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2308..2594)
                     /locus_tag="ACGE4U_002480"
                     /pseudo
     CDS             complement(2308..2594)
                     /locus_tag="ACGE4U_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2584..2913)
                     /locus_tag="ACGE4U_002481"
     CDS             complement(2584..2913)
                     /locus_tag="ACGE4U_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8394583.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2990..3262)
                     /locus_tag="ACGE4U_002482"
     CDS             complement(2990..3262)
                     /locus_tag="ACGE4U_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8394584.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3262..3525)
                     /locus_tag="ACGE4U_002483"
     CDS             complement(3262..3525)
                     /locus_tag="ACGE4U_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8394585.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            3759..4386
                     /locus_tag="ACGE4U_002484"
                     /pseudo
     CDS             3759..4386
                     /locus_tag="ACGE4U_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
BASE COUNT         1328 a          873 c          586 g         1599 t
ORIGIN      
        1 ttaaatgttc ttactgcctc ttccacacta atttcttcgt tcaatttcac taataatact
       61 tgatctcgct tattataatc gctttcattt atcctaagag tcaaatcttt ttccaccata
      121 acttttacac ccaataaatt ttgcggttta tttttctctg ttgaaattct ttgtgtagcc
      181 tttaaactga caattagctc attgggtaca tactctattt gagagtcatc taattggctc
      241 ataatccttt ctttttggtt agtatctgca gttacttgtt tagtatgagc catgaaccca
      301 aacatactca ccccaacaac caatcctaaa tatattttct ttttaaactt tttcatctac
      361 atctcctcct aatagtttta ttttagagtt agctctatta cttatttact aactcaacaa
      421 cagattatca tacacttgtt aatgaaatca caatatctca ttaaaacaca ccctaaaatc
      481 ataaccgttc ctatgctcat tttttggatt ttttaatcac gtaaccagaa aaataaatcc
      541 cctctatttc acgcaaatca aagaagaact aagtaactac cgtttaaagg tagttactta
      601 gttcttcttt gattattatc taactttatt attcattccc caccctttgc gcagtgagtc
      661 ttcggtatct tgttttatct gactggtagt tcttattatt tctccacgat catctaattc
      721 tggaacaatt ggatattttt ctctgaacaa gtctagttct ttcaccacgt cattaaacaa
      781 atcttgctta tcttcttgcg taagattctg tttaatatac tcatctaaag cttttctaag
      841 caattcatct cttgtcatat tagtctcata cttttctact aaaactctgg aagtcgcatt
      901 taacaaagtc gacagtttta aaggatgctt ggtggaataa accttatcaa actttctgat
      961 tggtgcgcca actttttttg ttcttcctct actctgtctt tcaacattgg aggaagtttt
     1021 tatttcttga acttggtgca cttctaattg cttattttca gatggtgtaa ttgcttgctg
     1081 ctttttcttt gcttctgctt ctttttttat tttggcgagt cctttgttgt tcccaaaaat
     1141 atcaagtcct ttgatgtcag ccaattactc cacctccgca taccaattct cgtgttcttc
     1201 caattctttc aaaatactga taaataattc gtggcttttg cgatcccatt gttttaagag
     1261 acctttcttt gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta agcgcgcatt
     1321 tttgagtaca acatttttaa tcaaatgttc cccataaatc tcttgggctt gtttatacat
     1381 ttcctgatct actgcgtcac cgctttccaa catgaaagga ataacaccag caacatataa
     1441 atttgtatca aattctttca caaaaaagtc catatactca agatattgca tcgcatttct
     1501 catcgatttt acttgggtct gtaagacgac gattgcccaa tccgcaatgt acatcgcgga
     1561 tttactatat acagaaatag aaggtgggac atctaaatag atgacgtcaa actcatcaaa
     1621 taatggttct agcttttctt tcatatattt gaattgggat aatgaatcat tatattggtc
     1681 tttgatccaa tcatcataca tttcaaagga catgtctgct ggaagtatat agaggttatt
     1741 gattacttta tagacagcct ctttaggtgg aagattgttt atcaacacgt cagtcacagt
     1801 attttcgtaa acaatcggtt caccttctcc gcctccttct tcgttagtga agttcatatg
     1861 cgttaagttc atcacatcag aagtatttcc ttgcatatcc aagtcaatta tcaaggttct
     1921 ttttccttca ttaactgctg attcaaaccc taacatttgc gcgcacgtac ttttacctac
     1981 gccaccttta aaattaccga ttacatattt cttcgctacc acgttcctac ctcttttctt
     2041 ttagcaatat tcatttgata ctattataac atatttattt ttagttacta ccaaaaaaaa
     2101 gatatatatg tactacctta taattaataa gtaaaaggta gtacataaat aaggaaaagc
     2161 tgaaatatat tgatttatag ctttcctcaa agtaatgacg ttatttaatt tatcgagaag
     2221 cagagaatac tcgcgacttt agtcatgtag acgaatcaga ttttcgataa aaaagaagcc
     2281 gtccttctta agaacgacaa caaatcacta gattaatttt ttcaactgtt cataaaagtt
     2341 ttcatgcgtt ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac
     2401 tctatatgtt gtgcccgcat atttgaaccc actagaccaa atacctgata aatccccagt
     2461 tttttgttca cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt
     2521 ttctttcaat ggcttctctt ttagcttttt aaatacttaa tgacatgttt tgaaggaacg
     2581 atttcataag ccaatttctg ttgccaactc ctctttagtc attactttag aattagtaag
     2641 tgtatcttca gcaatatctc taaaggaaga atgcattttg ttttttctat tcttaaattc
     2701 tacaagaatc tcatctttat cgtagccttc tgcaattata tcagacaaaa tatcttcgct
     2761 aaaatcataa aattcttttt gtggttcaac aaattcataa agaataccat tctctaccaa
     2821 ctttgcttca acttcgactc cattaggaac attaaaatct ttaggtactg tcaacatgat
     2881 tgaattgcct tgttttcttg tttttgtaat catttaaatc cctcatttct aacagtatgt
     2941 aattacttag taaatacatt ataccacgag ataatgtatt tacaagtaat tactgtgact
     3001 cgtaatgcgt ccttgctgaa taaatttcaa ccacgtgttc atcttcatct accttataaa
     3061 caacccggtg ttgtctattc aatcttctag aatacctacc ttcatgtgta ggttttaact
     3121 tctcaaatga ttgggtaggg agatagggat cttcctttaa agtctgaata accttctcaa
     3181 attgaggttt caaattagac tgtttgattt tctttaaatc agctttagcc gagtttttaa
     3241 tcatcacaga atagttattc attataagtt atcccaatca atttcatcaa cattagtaaa
     3301 tccactatca tctttttctc gcttacgaac aacacccaaa gtgcctattt gctcaagtaa
     3361 tagcgtttct ttgattgctt tccaatcatc caaaccaata agcaccgcat tattttcact
     3421 gcgctcacta atgatttcaa tttccgtatg attctcattt acatctttca acaacttata
     3481 aaaatttttc cgtgctgagc taggatttaa gactgttttt gccatatcta ctccccctct
     3541 aatttatatc tccattctaa cgtacttaat taagtacgta aattcgattg ttttgtttaa
     3601 attggagtac caaaacagca ttgtaaagtg ttatccgttt cctttatacc cgtggtgcta
     3661 gttaattttt tcctagagga aaatgtctaa acatgttggt aaatcaacaa aaaaacgcat
     3721 agagtagtct caaaactccc aagaaatgag gactacatat gcgttcatca aaatatactg
     3781 aacattatac agatactttt ataacattca aggaaattat gagaactgtt tctaacatat
     3841 accacaattg tgtaccagat aagattaaaa accgaagaaa tactgatcaa ctgaagtaac
     3901 gcgatacagt gattattgct tgtgttatat ggggcataat taatggctat actagccaaa
     3961 gagccacgta tagagcggtt tgttccgtct tatttcctaa tggtgacttt cctagtagga
     4021 gtcgattcac tcgcttaagt tcaaacttag cttacactat caagattatt cgatactttt
     4081 tcatcaagaa gctcaccaaa ggtgaactag tcggaattat agatagtttt cctagtccat
     4141 tatgtaaacc agttagaaat agacaagcaa aactattgaa tcaaatagcc aaagttggct
     4201 ataactcaac aaaaaaatct tatttttatg gtcttaaaat tcatatgatc gttactaaga
     4261 ctggctttcc gattacttac tcaatcacga atccaggtgt ccatgacgtg aaagtgttag
     4321 agactttatc ggaagaagcg aatcttccca atatcctagg ggacaaagga tatattagcc
     4381 ataaaa
//
LOCUS       DAXWLF010000187         4375 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXWLF010000187 DAXWLF010000000
VERSION     DAXWLF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.190"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(24..1172)
                     /gene="nagA"
                     /locus_tag="ACGE4U_002485"
     CDS             complement(24..1172)
                     /gene="nagA"
                     /locus_tag="ACGE4U_002485"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFF8394586.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1248..2084)
                     /locus_tag="ACGE4U_002486"
     CDS             complement(1248..2084)
                     /locus_tag="ACGE4U_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394587.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2268..3485)
                     /locus_tag="ACGE4U_002487"
     CDS             complement(2268..3485)
                     /locus_tag="ACGE4U_002487"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8394588.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3634..3795)
                     /locus_tag="ACGE4U_002488"
     CDS             complement(3634..3795)
                     /locus_tag="ACGE4U_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394589.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3868..3951
                     /locus_tag="ACGE4U_002489"
     tRNA            3868..3951
                     /locus_tag="ACGE4U_002489"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3902..3904,aa:Leu,seq:gag)
     gene            complement(4191..>4375)
                     /locus_tag="ACGE4U_002490"
     CDS             complement(4191..>4375)
                     /locus_tag="ACGE4U_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394590.1"
                     /translation="PTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFG
                     LTVLGICTFIITVINR"
BASE COUNT         1276 a          929 c          726 g         1444 t
ORIGIN      
        1 ttttaaggtt aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt
       61 gcttccaatg tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt
      121 tttccacatg tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact
      181 gcttgttctg gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc
      241 aatatacttc cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact
      301 ggaaattcgc ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta
      361 attaatgcga catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt
      421 acatgatggc catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc
      481 accatacctg gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca
      541 cttgcgcctg cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga
      601 gaaacaacta ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc
      661 ggtgctaaag caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta
      721 tccaaatcag gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag
      781 aaaggacctt caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg
      841 ccgattgtct cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa
      901 aatgaagtca ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca
      961 ttatccatta catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca
     1021 atccatttgc cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat
     1081 tcaccaaaaa gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa
     1141 aagaatttgt cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct
     1201 ctaaagtact cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt
     1261 gaaatttgtt tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct
     1321 ttacgcttgt atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc
     1381 ttctcttgag cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt
     1441 tgatggaata agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt
     1501 tcatcagctg attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc
     1561 gtttttcctg ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca
     1621 gaaacatgga ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg
     1681 gactgagaga tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt
     1741 tgaaagtcag ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct
     1801 aacttccgat aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt
     1861 gtccgagtag tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga
     1921 caaatctgca tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat
     1981 acactttgtg aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga
     2041 atctctttaa tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa
     2101 atatgcgtat tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag
     2161 acttgcaact aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc
     2221 agtgtgtttg tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga
     2281 taggttgcta ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga
     2341 agataaacga ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt
     2401 agaatatgat gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat
     2461 cgctttgtat caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga
     2521 ccaggaatat cattgattgc attatagata tattctcttt gttcatagat ccttccgcct
     2581 ggcaggagca gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga
     2641 gacaatacat tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct
     2701 ttgacgtgtg acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga
     2761 tgagatttgg atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc
     2821 ggaatatgtt ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc
     2881 aggttatttt gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc
     2941 ccagtaggat tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat
     3001 ttgatatcat caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt
     3061 gtgcctccag ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt
     3121 acttcatctc cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg
     3181 ttgccggtgt agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc
     3241 tcaattgctt ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg
     3301 gcattcatga tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga
     3361 tttcccgtgt tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct
     3421 agaacgggac cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct
     3481 ctcatcactt agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt
     3541 tactatttca atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta
     3601 agtattaaaa aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag
     3661 attcgctgcc caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa
     3721 acgtccgaat gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc
     3781 gtcatatttc tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc
     3841 tcgctataat tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat
     3901 tgagggtcat gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc
     3961 gtgaagttaa ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata
     4021 catatcactg tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta
     4081 tatgaaggtc ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa
     4141 aaacgagtaa aatccctcac ctaatactaa agcgagggat tttttgtata ttatctgttt
     4201 atcaccgtta ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca
     4261 tttttttgta cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg
     4321 ataacaacat aaagtatata gtacgtccgc attccggaag cccatactgt gggag
//
LOCUS       DAXWLF010000188         4266 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXWLF010000188 DAXWLF010000000
VERSION     DAXWLF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.191"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            30..1370
                     /locus_tag="ACGE4U_002491"
     CDS             30..1370
                     /locus_tag="ACGE4U_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8394591.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1588..2031
                     /locus_tag="ACGE4U_002492"
     CDS             1588..2031
                     /locus_tag="ACGE4U_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFF8394592.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2535..3953)
                     /gene="nhaC"
                     /locus_tag="ACGE4U_002493"
     CDS             complement(2535..3953)
                     /gene="nhaC"
                     /locus_tag="ACGE4U_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFF8394593.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
BASE COUNT         1269 a          805 c          833 g         1359 t
ORIGIN      
        1 attgtgaatt taaaagaaag aggtcattta tggaagataa aaaattgaaa aaacaactta
       61 gctccagaca tatcaccatg ttagctttag gtggagcaat cggtgcaggt ctatttaaag
      121 gaagtggcga agcgattggg atcgccggcc cttctgtatt gattgctttt ttgattggtg
      181 ggctgatttt atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc ggtggcgaca
      241 cgcatcacgg attatctggt ttgatacgcc cttatctagg tgctcattct gctgatttta
      301 ctgattgggt ctactggtct atgtggatta tcaatatcat cgctgaagct gttgcagctg
      361 cttccttttt gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt tttatgttgg
      421 ctgttttaac tacgatcatc aatctttatt cggtacggtt attcgcagaa acagaatatt
      481 ggctggcttt tgctaaaatc agtgtcatta tcttactgat catttttgct acttatcttg
      541 tcggtcagca aatgttgggc acaggcgttt tccctaccct tcagcaattg actgaccatg
      601 gtgggtttac tcctcatggt atgaagggaa tcatcagttc tttgcttgta gttatctatt
      661 cttacggcgg ttctgaactg attgctatca cggtaagcga agcagatgat cctaaacgtg
      721 cgattcctaa agcaatcaaa ggtgtcatgg gaagaatcat ttccttttac atcattcctt
      781 tgtttttgct tttgattctt tttccttgga atactttagc aggcacgaat gtcagtcctt
      841 ttgtgatggt atttgaaaaa atgaatatcc catttgcagc ggatatcgtc aattttgtga
      901 ttgtccttgc tttgttctca tctatcaatt cgggtgtgta tgcatcttca agaattttgt
      961 tcttccgttt gaaggatcga aaaggttcct ctagaaaact agctgtttta aacaagcatc
     1021 aagtaccgca acgtgctgtt ttattttgta ccagtgttct ttatcttggt gtagctcttt
     1081 cttattttgt cggtgacaaa ttatttggct atctggcagg ttccctttcg tatacagtat
     1141 tattgatctg gatcattatc agtgcggcat cctttgtact ggcactaaga aaaggaacat
     1201 tatgggataa aggaatcagc ttttttgcat tagctgtctt aggattgatt ttccttggta
     1261 tcctgttcac gaactccatt ggggtaactg tattgactgg cctgctttac ctgttcattt
     1321 atttcagtta tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga aatcgataga
     1381 aaattttatt ttatttatgt ggaatagatg gagctattga agaaaggagt tgtgacaaaa
     1441 gtcttgttgc aactcctttt tccgaataaa cagtctcatg acctcttggt aaacggtgtt
     1501 caaaaaccct ctgtgcaaaa taattgattg ttctccatgc tcatgacatg atagaagaaa
     1561 ggaatgaaaa aaaggagttt gttttaaatg aaaaagtatt ggaagttttt gttgatgatc
     1621 ggtgtttcaa cggtagttat gtttggtatg atgtacttga atgtctatgc gctcgatcat
     1681 atcttcttca gtcgtacacg agtattcatg gcgttgatga tgggtgcagt catggcagtc
     1741 atcatgctgt tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt tatgatctta
     1801 gccgctagtc ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca aactactgtc
     1861 aacgacacag cttggatgaa ggccatgatt cctcatcatt ctatcgctat cctggcaagt
     1921 aaacgagctg atttaaaaga tcctgaagta aaaaaattag cggaagaaat cattcaggca
     1981 caagaagaag agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg accattatcc
     2041 gtccaaataa aaaaagagga tgttccagaa gttatcagct tcaagaaata agaagaagct
     2101 acttctggaa cgccgtgaat aaggggttgt gaaaaagctg ccctgcatca agtaataaga
     2161 acagccaaac aaaaatagct tctcctattt ttcgtttggt taggcttatt accgcagatg
     2221 caagcttttg aacaccgttt attaagaggt tgtaaaatca gcgttctgac ctgagtatta
     2281 gagagacaaa tggaaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg
     2341 tcagctgatt gaacaccgtt tatcgagagg ttgtgaaaaa gtcgcttagt ttcgcgtatt
     2401 aagaaaaatt ttgtgaaatg gctttccaca tttttgtata aatttggctt aataccgaga
     2461 aactggcttt tgaataccgt ttattcgtaa aagggattgt gacaaaagtc ttgtcacaat
     2521 ccctttttat gtttttattg ttctaattgc gctgtcttgc ttactgcttc ttgcggtttc
     2581 acaaattttt ttgagattcc ccaacctgtg aaggcactga ccatcgaaaa cacaggagaa
     2641 agcaaactga aaaatacgaa tggcaagtaa gtgagtgtag gaataccaaa tgtacttgct
     2701 acgaaacttc cggcaattcc ccaaggcact aagtagttga ttactgttcc accatcttct
     2761 agcgttctac ctaatacagt tggatcaaat cctgcttttt cataagtttc tttaaatgca
     2821 ttaccaggca gaataacaga taggaattgt tcaccaataa aaatatttac tccgatactt
     2881 gtcatcaatg ttgtaaaaat caaatttggt gtgttcgtca tctttcttga tactcgttcc
     2941 atcacggttg agatcaagcc aaattcgatc aacaatccac ctaatgacaa agttagaata
     3001 atcaatgaca cagtaggcat catactttcg attcctccgc ggctaagcag tgaatcgatt
     3061 tgttggtttc cagtatttga aacaaaccct gattcgatga ccccagccaa cttcgttaag
     3121 gaagtttgtg ggttttgaat aaaaatcatt cctactgcta cagcgatatt caacaaaatc
     3181 gttaaaattg ccggcacctt tttccatgca cacagtaaca ttaaaaataa aggaatcagt
     3241 gaccagattg aaatagtaaa gtttgcttct agaacatcag tcacttcacg aatagatgaa
     3301 agacttgcgg ctttacttgt gtgtcctaaa atcatgaata aaaagaaaga aacaacaaat
     3361 gccgggacag ttgaccacat catatgcttg atgtgtttga acaaatcggc atcaacaata
     3421 gctgctgcta gatttgttga ttcggataat ggagacatct tatctccaaa aacggcacct
     3481 gaaacaatag ctccaacaac cagcgctgga tttaagccta gtgttgtccc aataccgaaa
     3541 aatgcaatcc caatcgttga cataacagta aaggcactcc ctacagcgct acctacgatt
     3601 gcacacacaa caaatacaga agggacaaac cactggacac tgatcaattt gaacccaatg
     3661 accattagag ttggaatgat ccctgcttgt atccatacag caatcaatga accgactaaa
     3721 ataaaaataa agattgggat gattcctggt ttgatgccat tgatgattcc ttcgtgcagc
     3781 caatctgtcg ggtatctttt tatcactgca tacaacatga ccattccgat cgctgctaag
     3841 atcgtgatat taggtgagac ctttaatccg acaacgccaa tcgcaatgcc taaaacaata
     3901 accattaata caagtgttgc ttctaaaaaa cttggtgttc ctttttttcc catgataatc
     3961 tctccttttt tagttgttct tatgttctaa aaaagttaag atatcggatt actccgccgc
     4021 aattcatccg cgattcctcc tttataaaaa taaaaagacc tgttttaaac taaggacgaa
     4081 tgactccgcg gtaccacctt aattaaaaac agatctgttt ttcactttca aacttttatt
     4141 caaatgtatg gactttccat gtaattcgtc aattaggctt gatcggttca cagcgaccac
     4201 cgactttctg aacaaaacac ctaaccgcta ctgcgtgaaa agttgacttg tacgaagttt
     4261 accttc
//
LOCUS       DAXWLF010000189         4248 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXWLF010000189 DAXWLF010000000
VERSION     DAXWLF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.192"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            35..958
                     /locus_tag="ACGE4U_002494"
     CDS             35..958
                     /locus_tag="ACGE4U_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFF8394594.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            964..2217
                     /locus_tag="ACGE4U_002495"
     CDS             964..2217
                     /locus_tag="ACGE4U_002495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFF8394595.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2351..3697
                     /gene="gor"
                     /locus_tag="ACGE4U_002496"
     CDS             2351..3697
                     /gene="gor"
                     /locus_tag="ACGE4U_002496"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFF8394596.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1462 a          678 c          924 g         1184 t
ORIGIN      
        1 tgctactatt atgaagttgt aggaggagaa aattatgatc gaactcataa ccacagtaga
       61 aactattgaa caagcagaac agttgcttgc tgcaggtgca gatacactat atttcggtga
      121 agagcaattt ggattgcgtc tgcctgcttc atttacacga gaagaacagc gacaattggt
      181 agaattagca cacaagtatg gtaaaaaagc gaatattgct gtaaatggga tcattcatcc
      241 tgaaaaaatg aaaaatgtac ctgaatattt ggctttttta aaagaaataa acgtagatgt
      301 gatcacagtt ggtgatacag gtattattta tacgatgaaa aaaaatcctg aattgcgtat
      361 accgtatatt tatgatgctg aaacattagt aacgagcgca cgtcaaatca atttctgggc
      421 gaaaaaaggc gcagttggag cagtacttgc tcgtgaagtt ccttttgaag agatgaaggc
      481 gatggaagaa aaactcgata ttcctgtaga aacccttgtg tatggtgcta catgcatcca
      541 tcaatccaaa cgtccactgc tacagaatta ctataactat acgaaacaag acgaacaaaa
      601 agatcgtgaa agaggtcttt ttatctcgga acctaaaaaa gaagagacac attattctat
      661 ttatgaagat agtcatggca cacatatttt tgccagcaat gatctgaatc tctcgaatga
      721 attgaatgaa ctttatcagc atcattactg tacatggaaa ttggacggat tatttactcc
      781 aggcgaaaaa ttcgtaaaca tcactcgact attcgccgaa gcaaaaaaac aaattttaga
      841 aggtagctgg gatcaagaaa aagcggaaaa aatgactagc gaaatcagta aattgcatcc
      901 tgaaaaccgt gaattagata caggattctt ttatattgat ccttctgcta tcaaataagg
      961 agtatgatga aagaaatgac agtaaaaaga aaattgaaac gaccagaaat cctagcgcca
     1021 gcaggaacac tggaaaagtt aaaaaccgcg atccattatg gagcagatgc agtatatatt
     1081 ggcgggaatg catatggttt aagaagccgt gcaggtaatt ttacttatga agaaatggct
     1141 gaaggagttg cttttgcaaa agaacatgat gcgaaggtat acgttgctgc taatatggtc
     1201 acccatgaag gaaatgaaga tggagcaggg gaatttttcc gcagtttgcg tgatgtcggt
     1261 atttctgcag tgattgtttc tgatccagct ttgatcgaaa tttgtgcgac agaagcccca
     1321 ggtctgccga tccatttatc cactcaagct tcagcaacta attttgagac attggaattc
     1381 tggaaagaag aagggttgga acgtgtcgtt ttagctcgtg aagtttcaat ggaggaagtg
     1441 gcagagattc gtaaaaatac ggataccgaa atcgaagcat ttatccatgg tgccatgtgt
     1501 atctcttatt ctggtcgatg cacattgtcc aaccatatgt cgatgcggga tgcaaaccgt
     1561 ggcggatgtt ctcaatcttg tcgctggaag tatggattgt tcgatatgcc ttttggtcaa
     1621 gaacgaaaca gtttaactga tacaggcgaa gtggaagaag aattctcgat gagtgcagtg
     1681 gatatgtcaa tgatcgaaca tattccagaa ttgattgaaa acggcgtaga cagcttcaag
     1741 atcgaaggaa gaatgaaatc gatccattat gtatctacgg tgtcaaatgt ctataaagcg
     1801 gctgtagaca gttatatgga agatcctgaa aactatgtct gcaagcaaga atggatcgac
     1861 gagttatgga aagtagcaca acgagaatta gctacaggtt tttactacaa tacaccaact
     1921 gaaaacgaac aattattcgg tgaacgtcgt aaaatcccag tatataaatt tgtcggtgaa
     1981 gtattatctt atgatgaggc aacacagatt gctacgatac gtcagagaaa cttgttttct
     2041 gtaggagatg aaatcgaatt ttatggtcca ggattcagtc atttcagtca aaccgttgaa
     2101 gttatgcata atgaagaagg cgaatccatt gaccgagcac caaatccaat gatgatttta
     2161 actatgccag tcgcacagcc ggtgaaacct ggagatatga tccgaaaacg taaataagga
     2221 aaaagctgat atatccctgt tttaagcatc ttcgcataca aaattcaaga aaagtgttac
     2281 acttgttttc tgtagaaaaa ggatcgttaa ataaaggttt tgattgatct cttcaataag
     2341 gaggaacaaa atgcaaatat atgattacat cgtaattggt ggcggctctg gcggtattgc
     2401 ttcagcaaac agagcaggga tgcatggcgc aaaagttttg ctgattgaag cgaatgaatt
     2461 gggcggaaca tgtgtcaacg tcggttgtgt acctaaaaaa gttatgtggc aagcaagtac
     2521 gattttggaa acaattgaac gagatgcctc aagttacggt atccaagcag atttaaaaaa
     2581 tgttgatttc gccggtctag tagaaagacg agaaaaatat attgattttc tacacggtgc
     2641 ttatcaaaga ggattagata gcaatcatgt cgaggctata aaaggttacg ctacttttat
     2701 cgatagtcaa acgatcgaag taaacggtga aacctatcgt gctcctaaaa tcttgatcgc
     2761 tactggaggc cgtgcaagta aaatgaccat tcctggtgga gaatacgcaa ttgattcaaa
     2821 tggatttttt gaattaacag agttacctaa gagagcaatc gttttaggtg ctggatacat
     2881 tgcggctgaa ctttcaggtg tatttcgtgg tttaggtagt gaagtcatgt gggcatatcg
     2941 aaaagaacga ccacttagaa cgtttgataa aatgctttcg gacaatttga tccaaatgta
     3001 ccaagaagct ggaataaaaa cttacgcaca tcatattgct aaagaaatta ctaaaaacaa
     3061 tgacgaatat acggtgctat ttgaaaatgg tgaaacctta actggtgatt gtgtactttt
     3121 tgccggtgga cgtgtaccga ataccgaaaa attaggtctg gaaaatacaa atgtggaatt
     3181 ggacaaaaaa gggtttatca aagtagataa ataccaaaat acaacggatg aacatatttt
     3241 tgctgttggt gatgtgatcg gtaaattaga tctgactcct gttgcaatcg cagctggtag
     3301 acgtttatca gaacgtttat ttaatggaaa gaaagattct tatcttgact ataaacttgt
     3361 tccgacagtc gtctttacgc atccaccaat tgcaacaatt ggtttgacag aagaagaagc
     3421 attggaaaaa tatggcgaaa atgaattgaa agtttatcgt tcacgcttta ccccaatgta
     3481 ctttgcatta aatgattatc gccaaaaatg cgaaatgaaa ttgatctgtg tcggaaaaga
     3541 agaaagaatc gtcggcttac atgctattgg tgttggtgtc gatgagatgc tgcaaggttt
     3601 tgccgtggct atcaagatgg gtgcaactaa agaagacttt gacaatacag tagccatcca
     3661 tccaacagga gcggaagaat tcgtaacgat gcgataattt aatattaaca gttccaacag
     3721 ctttttattg aacaaaccat gtaaaaccag aaaatcagta tactgatttt ctggtttttt
     3781 gttttttagt tatttatgta agattgcttc gataattctc aaaacgaaat aagtagggaa
     3841 gttggagatt ttgattatct tcgtaactga cgaagagtaa atgaatccat ttggtatgca
     3901 ctcttaagtt caaaatattg gtatcgatgt ttaaaagaaa tagaaatgta tcgaagaaca
     3961 gatgtttaaa aaagtgtgac tttgtataaa taaattagaa gaagcaaacg aggatgttaa
     4021 aagaatacca tatagttacc aaaccaagaa agttctcgtc aagttcgctg gtacgaatca
     4081 aatcaagtga aaaaagggac tgtgaaaaaa attgtgtcat agttagtcac tttttccgaa
     4141 caaatagtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa aatatgagga
     4201 actatttttc catttgtttc tctaatactc aggacaaaac actgattt
//
LOCUS       DAXWLF010000190         4215 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXWLF010000190 DAXWLF010000000
VERSION     DAXWLF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.193"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            57..1481
                     /locus_tag="ACGE4U_002497"
     CDS             57..1481
                     /locus_tag="ACGE4U_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFF8394597.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1662..2435)
                     /locus_tag="ACGE4U_002498"
     CDS             complement(1662..2435)
                     /locus_tag="ACGE4U_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8394598.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2432..3337)
                     /locus_tag="ACGE4U_002499"
     CDS             complement(2432..3337)
                     /locus_tag="ACGE4U_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8394599.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3491..3811)
                     /locus_tag="ACGE4U_002500"
     CDS             complement(3491..3811)
                     /locus_tag="ACGE4U_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFF8394600.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1288 a          765 c          834 g         1328 t
ORIGIN      
        1 aaaaaaggtt tgttatactt tttgataaaa gcggttttat tttggaggat gcttacatgg
       61 atacagagca acagaaaaaa ggaattggtt taattccact tgctgcctta gtaattggtt
      121 cagcgattgg aggtggcgta tttggtatta tgacggacat ctccggatcg gctggcggcc
      181 cagctatttt cagttgggta ctcgtaggaa cagcaatgtt gatgctctcc ttgtcgatca
      241 ataatatcaa taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag
      301 gttttggccg tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag
      361 gcaacgtagc ttttgcaaca ttgttgatga gcgcattagg atatttcttc ccagtatttg
      421 aaggtgggca aaatctggca tcgatcgtga tgagtacagt ctttttatgg ggatatgcat
      481 ggctagtgaa tcggggagtc gaaaatgcta gtttcgtcaa tgctatcgtc acattctgta
      541 aattagtgcc attattttta tttattgtag tagctatcag cacgttttcg atccaacaat
      601 tcattgataa cttttctagc gacattatat tgaatgcgaa tggacagccg atcccgtttt
      661 ccagtcaagt attgagttgc atcatggtga tgctttgggt atttgttggt attgaaggag
      721 cctctgtcgt aggttcacga gcaaaaaaac aatcagatgt tgggaaagca acgattctag
      781 ggatattcgg cttgattatt atttacgtct tggtttccat gcttccatat gggacaatga
      841 cgatgaatca gctgcaaacg atcaaggcgc agcctgctat gggctatgtg tttgaaatga
      901 tggttggtaa atggggagca gtcgtgatca acggcggttt gatcatttct ttagttggtg
      961 tttggctctc atggacgatc ttaccgattg aaacaatggg aaacatggct gaagacggac
     1021 ttttgcctaa agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat
     1081 tgacgacgtt atgtacgaat gtctttttac tttcattatt attcactgac agtgcttata
     1141 actttgctta cacacttggt acagcagcta ttttcttcac gtggcttttc ttaggattgt
     1201 accaaatgaa actttcttat cagcggaaag aatgggtcca atttttagta gggcttttag
     1261 caagtgcctt tcaagtatgg gctatgctag tcgtagcctt taatgaagta atgcttgttc
     1321 ttgttctttt cttgccaggc atttattttt acctcaaagc acgtaaggag caaaacgagc
     1381 ctgtacaaca tatccactta gataagattt tacttatagg tgtgacgatg gtcggagtga
     1441 ttgcaatggt tttatttgcc actgggatgc ttcacgtata gaagtataaa agaagtataa
     1501 agagaggttg tgagcctgtc gtttattcaa aaacatacta ggaaaggagc tgtgacacaa
     1561 atgttgtcac agctcctttt tcagaataaa cggtgtttaa gagacagcca atcggcaata
     1621 aggctgaacg tctctttcac agcctctttt ttgatgagtc atcatacatt ttttcgctta
     1681 aatacgagat atcctaatgc taagaacaaa acgatgtaag ctaaattacc gaatagcatt
     1741 tgattagtag aaagatgagt caattgcttc atgatctctt cgtttccaat ttggttttgc
     1801 agattcaaca tactgatcgg attccatttg atccactccc atttttggat gagtgcaata
     1861 aggataccag atatgattga cgaagcaaaa taaaagacga ttcctgctga gatggatgcg
     1921 ccggaactat tgataaagca agcaatcatc aacactaaac ttaaaatcag ccataaaccg
     1981 atataactac ccaaagtata agtgaacaag ctttggatca gtgtttgacc agtagaaacc
     2041 ttctccgtga aggaaatcga agggaaaaga atcagtttca tcaacacagt gacaataata
     2101 gctaaaaggt ataaataaac agaataaata actaatgtga tccatttgct tactaatatt
     2161 tctcctcgag aatactttcg ataaaggaga tttttcaatg taccatattg tgcttccatc
     2221 gaaatgatcg tgcttgcagc tgcaatcatg ataaagacaa tccaagatgt ggcactaaaa
     2281 agctgagcag tcatttcaac tggatcaatg atcgtcgtgt attttttggc tagaagtgct
     2341 gttcctatga gcaaaacaac taatagaaca gataaaacag cagttgactt tttcttgatc
     2401 aatttaaata attcttgttt tacaagcaat gtcatttttc ttcttccccc tttactgaat
     2461 ccagaatttc caatagagaa gattccaaat cattcgtcag atgctggacg tctgtcacat
     2521 ggatattttg tgcagacaat agctggatca cggttcctaa taattcttcg ttttctttta
     2581 aaagcttgat tttatcagga tcttccaaag tatatccagc ttctgtcaag atcgttcgag
     2641 cagctagatc atttgttgta gagataacga tgaaatcttg cgctgtttgg ttcaattcag
     2701 ccattgtaca ttgtttgatg atctttcctt gattgatcag gatcagctca tcgaccattt
     2761 tttcaagctc gctgagtaag tgactggaga tcaagaaaga ggtgccctga gtagccaagt
     2821 cggtaatgat ttttcgcaca tctcttgttg cttgtggatc caaaccgttc atcggctcat
     2881 ctaaaataac gatagacgga tggttcagta aggcaagagc aattcccaat ttttgtttca
     2941 ttcccaacga gtaggatttc gcttttttgt gaatgtactt ttccatcttg agctgattca
     3001 cgatagtgtc gatagcagat acatcgttgg tttcagagaa taacttcaga tgatcatagc
     3061 ctgttaagaa tggatagata cccgggtatt cgatcaatgc tcctacttgt tccaaccctt
     3121 tatgactaga gggagaaatc tcattttcac caatacagat tgttccatct gagtaatgga
     3181 tcagtcctaa aatagctttc atgatcgttg tttttccagc gccattagga cctactaatc
     3241 caacgatttt gccaggggat aagctaaaac tgacatcgct gagggcttgt ttgtgaccaa
     3301 agttcttgga taattcttgg accgaaagac ttttcaaaaa tattcactcc ttttctatag
     3361 atagtacaag catacacctg atgagtggag aagtcgctag gtcattggac ttatttctta
     3421 ttttttcata aaaaaattga atattaaata aaaaggctta ttttttctga tcagtactta
     3481 ttccaagtgt ctagtggccg ctagtaatct tgatgccaat gatactgatg agtagcatcc
     3541 ccacaaagaa ccaagtggaa agattcagct tatcatgaaa caagaccaca ccaattatga
     3601 ccgaaccaac tgcgccaatc cctgtccaga taggataaga gagactcatc ggcaaacttt
     3661 tgattgcctt tgacaaaaag taaaaactag caatcatgcc aatcactgtg taaaaagaat
     3721 aattgatttt agaaaaacca tcactccatt tcatcatcgt tgaccagaat acttccaaaa
     3781 ttcctgctat gattaattcc atccatgcca tacttacatt cctccttaac tataaaaaaa
     3841 tacacaaaaa gaggaaaatc atcccttcaa agacctaatg gaatgatcat cctctttatc
     3901 ctacccggtg gcagattgta tatctttttc tgtagtatag cacacctatc cagatatcgg
     3961 caatctcata ctttttagta aagtgtacca agcactgacg gatatatcgt tagcggaaaa
     4021 gaagatgaat gctttgcaat ttcatagaaa gaagttgtta tttattaatc atttaaaaaa
     4081 aacagtaact agaccttatt tctatatatt atcgggcaat aaattaaaaa aagctttacc
     4141 ttacaataag taagataatt tttgtgataa aaaaataacg aaaaatattt aaaaatagtg
     4201 tctatttcgt gagaa
//
LOCUS       DAXWLF010000191         4156 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXWLF010000191 DAXWLF010000000
VERSION     DAXWLF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.194"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..298
                     /locus_tag="ACGE4U_002501"
     CDS             <1..298
                     /locus_tag="ACGE4U_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8394601.1"
                     /translation="FSLYPEKFFLVKDLWIAVMQNSSNKLPKTYNHIAFKIDEQDIDS
                     FVSKIQMLGLTVEPGRSRVKGEASSIYFYDYDNHLFELHTGTLQERLKSYNSTT"
     gene            complement(570..860)
                     /locus_tag="ACGE4U_002502"
     CDS             complement(570..860)
                     /locus_tag="ACGE4U_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394602.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1262..1498
                     /locus_tag="ACGE4U_002503"
     CDS             1262..1498
                     /locus_tag="ACGE4U_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394603.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1505..3457
                     /locus_tag="ACGE4U_002504"
     CDS             1505..3457
                     /locus_tag="ACGE4U_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFF8394604.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3529..4026)
                     /gene="dfrG"
                     /locus_tag="ACGE4U_002505"
     CDS             complement(3529..4026)
                     /gene="dfrG"
                     /locus_tag="ACGE4U_002505"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFF8394605.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1409 a          733 c          711 g         1303 t
ORIGIN      
        1 attttcatta tatccagaaa aatttttctt agttaaggac ctatggattg cagtaatgca
       61 gaattcgtcg aataaactgc caaagacata caatcatatt gcttttaaaa ttgacgaaca
      121 agatatagat tcgtttgtct caaaaatcca aatgttaggt ttaactgtcg aaccaggacg
      181 ttctcgtgtg aaaggcgagg cttcttcaat ttacttttac gactatgata atcacctctt
      241 cgagttacat accggtactc tccaggaacg tttaaaatcc tacaattcca ctacctgaga
      301 ctagtaatga ctagtctctt tttatggcct ctgatccccc cattgagcga atactaatag
      361 ggcttcgaat tgatctgttc aatctttaag caatatccct caaatgagtt tggaaagttt
      421 agtgattcat cacaaatact taaatacatg tagttatata tagattgagg ttcttgtgag
      481 cgtatgctcg tgtgggttca aatcccatcg tctgcatcca tctttccaaa acaagcggct
      541 aggacatttt tgtctcagcc gcttgttttc tagtttattc tttcatcttg tcggaccttt
      601 tttcttgggt atagactttt ctcatattct accacaattt tgggtttccc taacacatga
      661 taggatctca caacaaattg ctttctatta ttataataac ctgtaacgac taacttcatc
      721 ccttcgtcta cttcagctag gaagttcaaa ctatgggaag caatcaaaca attgacccca
      781 tttaaactga aacggatcaa cggtctggca ctaacttgca aagtacggat cttatctact
      841 gttccatatt ttttctccat aaccttcacc tcaataccat tatacgaaca tacattccct
      901 ttgtaaagaa gaaactctgt tttatctttt tgactttttg tctattacct ttcttaaaat
      961 catattggca cttcctgcga agatgaagga catgtactaa aaaaaagagg cagcagtata
     1021 gatttaagta gttcggtttt ggagtacaaa aagtgaaaat gtaaacctaa atgagtatca
     1081 aattgcaact gaatttgata ctcgtttttt attgttaaat caacgtttgt tgttaattta
     1141 caccacaaaa tgtaagttaa aatgtatata aaaatgcgaa cgtgaaataa ttattgtagg
     1201 aatgcaaatt ggtgtgttaa aatggttaca atttactaag caaaaagggg tgcattttcc
     1261 tatgcaattt cttgagccaa aaaataagaa cgctaagtct gtggattggg aaatatcaga
     1321 gcaagtaagg gtgatagtta agcaatacgc tgaatatgca gagcgtactg aaagcgaagc
     1381 agtggatgaa ttcttattga acattttaga cgataagaag tttattgaat ggattgccaa
     1441 caagcgaagt aataagcgga ttgttgaaaa aatgggtatc aaagatagag tgggttgata
     1501 gacaatgacc aagcttaaaa gattagccac gaaggaagat gaaattgtag atgttaaaat
     1561 tccgatttca aacgaagaat taaaagatag ggctaaacaa tatgatttat taactccaaa
     1621 acgatttgca acaaggtata acaaaatgtt gttcttgcca acgtctttta aatggaacgg
     1681 ttctgaatat cccattcaat ataattactg catcaatccg ttttgttgta attttgggaa
     1741 agaacagcac aaattcaaag atgtaaaagg gaaaccaagt cgatataaaa tgactggtag
     1801 ttccaaagat aaaggtcata aaggtatgta ctgcaatgac aatcctattg gcagaggtgt
     1861 atcgcaaaac tgtacagtaa cccctttatc taattggtct gttgttgaag aaattaaacg
     1921 ccttatagaa atcaatagca tccaagatgt tgagccagat tatcaatttc ataaagaagg
     1981 ctgctcggaa gaagaatcaa ccccattcaa tgagccgaag caattttaca aaagggggaa
     2041 aagtaggggc aaatctcaac gctatcaatg taaggcgtgt aagaagttca ctaatgttct
     2101 accaaaaaga gaagaaacca ctacttatca ccaacaaaag aacaccattc ttccaatgtt
     2161 cgctaagatg gttgttggaa gggtttcagt cagtcgtact tgtgatattt taggaattgg
     2221 agttggaacc tactatcata agctggagtg gctgtatagg cgttgtttgg agtttttaga
     2281 acgatatgag acacaacccc ttcaaacaaa gaaatttaac gagatgtggc ttaatacgga
     2341 caagatgcac tactacctaa ataacgtccg taagaaagga caaggttcaa agaaatatac
     2401 ggggtttgaa gaccttaata tgcaaacgta tatagttgta tctgctgagg tcttatcaag
     2461 atatgtgttc cgttctgatg ttgcctatga ttggaatatt tcaatggatg aattaaatga
     2521 agatacaaga aaattcaaag aagaccatct caatactttt agtcgtaaaa atgataggtt
     2581 agattggtct tattatccac aagaaccatc cgctaacgat agcgaaaata ggaatgctta
     2641 tcttcacgaa ttagggaaaa ttactaatag gagtagattt gtcgatggtt taaatgttga
     2701 tgctccttat actacaactg ctcactattg gttaattaaa caaatggtta atgccgacga
     2761 atggcgaatg attagtgatg atgacttctc tattagaaac gccttttata gagtattcac
     2821 gaaagaatta aggttgagcg atgcacatca ttttatttgc caagttaaca agacgaaaag
     2881 cagaaaacaa tgcttaaaag aattcggaca agcaaaagca gaactgctcg attggggaga
     2941 tatcagagga ttcaaaacaa agtttctacg gactttggca tctcattatc taactgaatt
     3001 actaacaagt catcaattcc acgaagaggc tattagtaaa gatggagaaa gatatcgcaa
     3061 atatgccgat aaccctatca aacatccatt agccactaag gataaaggat tttattcagt
     3121 tgattgtaga acggatttat ctgctctgga accaaacgaa atcgcaaaga tgctattaaa
     3181 tgttaatgac cattcgacca atagctttat tcaacagata agacgttata tatcatctct
     3241 tgaaagacca ttaacaacag ctcgtggtga taagaagagc tatatttacg ctaacttcaa
     3301 tccgaagtat gctcaatttg cgattacaat tctaagaact tactacaact tttgtcgccc
     3361 ttttaaatca gcggataaaa aggttttgac acctgctcaa agattaggga ttacagataa
     3421 acaatttgat tggaaagata taatttattt caaatgacaa aaagagcttg gagtaatccc
     3481 aagctcttgt gctttatctt tactgttctt cttcaattaa cgcacccgtt aactcaataa
     3541 gttttttctt tcatatacat gaaaatagta gttatacgga tttttatcat tctttatccc
     3601 tttttgggca aatacctcat tccattcctc ataattcact tctggaaaaa aagtatctcc
     3661 ttcgaattca tgatgtattt ttgtgatgta cattttctca acataaggga aaaacaaatt
     3721 ataaatctgt tctcctccga aaataaaaat ttcttcttcg tttttacata actcaaaaac
     3781 atcttctatt gaatgaacaa tttcacaacc attaaaggta aaccccttat ctctcgtcag
     3841 aataatattt cttctgtcag gtaaggctct tccgattgat tcaaggttct tcctacctaa
     3901 tattatcgga tgtccctttg tagtattttt aacatattcc cagtccttgg gaatcctcca
     3961 aggaatgtca ttctctttgc caatcactct attcttatcc atcgcagcaa tcaaagaaac
     4021 tttcatttat aacacagcct ttttaactaa tttaccatct aatttcttat ttctatcaaa
     4081 aaaccttttc tcattatcct gcccgttagt tgaataagca attttttcta taagttatat
     4141 tatcataata ccacaa
//
LOCUS       DAXWLF010000192         4152 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXWLF010000192 DAXWLF010000000
VERSION     DAXWLF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.195"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(27..167)
                     /locus_tag="ACGE4U_002506"
     CDS             complement(27..167)
                     /locus_tag="ACGE4U_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394606.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            complement(298..1059)
                     /locus_tag="ACGE4U_002507"
     CDS             complement(298..1059)
                     /locus_tag="ACGE4U_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFF8394607.1"
                     /translation="MNQPQIFNFEHNEVRTFLENDVPYFVANDVAKTLGYKNPSKATN
                     DHCKKAIETWGNDSLGRRQKFKIIPESDVYRLIIKSNLPSAEKFESWVMEEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQQVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            complement(1108..1341)
                     /locus_tag="ACGE4U_002508"
     CDS             complement(1108..1341)
                     /locus_tag="ACGE4U_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFF8394608.1"
                     /translation="MSTMEKKKNLRVEFLKPKLKLKKARLANEWTTSYVADLIGLERR
                     QYELKEKGEYPFHDYEMYILAKNFNKKVSELFF"
     gene            1483..2235
                     /locus_tag="ACGE4U_002509"
     CDS             1483..2235
                     /locus_tag="ACGE4U_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFF8394609.1"
                     /translation="MKKNILGSVIKDARKKKKLTQEQLSKLTGYSQNTISNHENGNRS
                     LDEDNIKTYATALDLTPDDLFEALDIKNSLENKMMIIDKKQTKIDNLLDLYSQLEESR
                     QTKVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFER
                     VPNNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDY
                     ESQTIILHSINELYEDRVVSPEQIRIIGKVVF"
     gene            2312..2521
                     /locus_tag="ACGE4U_002510"
     CDS             2312..2521
                     /locus_tag="ACGE4U_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFF8394610.1"
                     /translation="MPNYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESLN"
     gene            2628..3281
                     /locus_tag="ACGE4U_002511"
     CDS             2628..3281
                     /locus_tag="ACGE4U_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394611.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSLTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            3469..>4152
                     /locus_tag="ACGE4U_002512"
     CDS             3469..>4152
                     /locus_tag="ACGE4U_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394612.1"
                     /translation="MKKKKGTQLSVDSPNWLLIITVLCFAAFIMVSPQVYLHSVILGN
                     DIMFHFNRFYETYMQIKTGNFNFFQSLYSFQSSGRIITAFYGADFAYLQGLLLIILKS
                     WFKYQLVSSFSCFFIAGASMFWLTYKCKVRTNIGLIIALLYMSSSAVSYYPIAQAFTG
                     WGAALMPLLFVPAIEALKNKERPIRPLQLAIPVTLLLSTHLLSLVIGILAILPFYVIA
                     FINTKNKLNM"
BASE COUNT         1340 a          734 c          707 g         1371 t
ORIGIN      
        1 cttccagaag accgtaaaaa cgatgactaa acgtgagcta aaatctctgc tatggctgca
       61 accatggcag agtcttcttt atctaccgcg tgttccatcg ccacttttgc ttgtttcagt
      121 atgtctaatt tcaaatcttc gatttttgcc gagactgttt tttccatatt cttctctcct
      181 ttcttttata tctttccaaa agaatagtct atgaagccag ctcatttttg agttggtttt
      241 tgtaattttt aggtcaatgg ttacgcgccc ttttggtctc tccattcatc tcacctccta
      301 tgctgtttgt tcacgttcaa tcatcggtaa gtaattattc ttcttcaatg tttcatataa
      361 gaaaatgtgt cctttttgcg tccatttcat ccttgttttt acttgccgat tgccgttttt
      421 atcttcataa tcaaatggct caatatgcgt gtatcctttg tcttggaact tgctgtatag
      481 cagccatgtg ccactttgat tgaactggat acctaattca tgtagcaatt tattaaacga
      541 tacagcgctc ataccgtagt tcttagcaat cgtactaact gtaactaatc ctttgttgtt
      601 caagattcgg tcgtaatagt ctgccttcgg ctgtaattca tttacttgtt gttctgctat
      661 caatcgtaac gtgcgttctt ctttgagttt ggttgctact tcgattagca agtctggatt
      721 gttaagtagt tcatctgttg cgtacatgcc gtgttttcgg atttgaggta ggacttcttc
      781 cattacccaa gattcgaatt tctcagcgct tggtaaattc gatttgataa tcaagcgata
      841 aacatctgat tctggaatga ttttgaactt ttggcgacga cctaacgaat cgttacccca
      901 tgtttcaatc gcttttttgc aatgatcatt agtagctttg cttggatttt tatatcccaa
      961 agtttttgca acgtcattcg ctacaaaata tgggacgtca ttttccagaa acgttcgaac
     1021 ttcgttatgt tcgaaattaa aaatttgtgg ttgattcatt tttttacatt cctttcttgt
     1081 ttttatctca aatagagata tttgatttta aaaaaataat tctgatactt ttttgttaaa
     1141 gttttttgcc aaaatgtaca tttcataatc atggaatgga tactcgcctt tttcttttaa
     1201 ctcatattga cgtctttcca aaccaatcaa atcagctacg taagatgtag tccattcatt
     1261 tgcaagccta gcttttttga gttttaactt cggcttcaaa aactcaactc ttaagttttt
     1321 ctttttttcc atcgtactca tataatcgcc tccttcattt gataaatcaa taatatctca
     1381 tattgagata tatgtcaata gttttttatc actttttgag atatttttat ttacacgttt
     1441 atcacaatgt gatattgtta tctcatgaag gaggtgcctt taatgaagaa aaatatttta
     1501 ggttcggtta taaaggacgc aagaaaaaag aaaaaactta ctcaagaaca acttagtaaa
     1561 ttaactggct acagtcaaaa tactatatct aaccatgaaa acggaaaccg atccctagat
     1621 gaagataata tcaaaactta tgcaacggct ttggatttaa ctccggacga tctttttgag
     1681 gcgttagata ttaaaaattc tctagaaaac aaaatgatga ttatcgataa aaaacaaact
     1741 aaaatagata atttattaga tttgtatagc caattggaag agtctcgcca aacaaaagtc
     1801 tacaactttg ctgaacagca actcgaagag caaaatcgag gaaacgtggt gcactttcct
     1861 aaaaaggaaa aacttccgac aatcaaaaat tcagcttctg ccgccaaccc gactgaatta
     1921 gtttatggcg atacagttgt agaggaagaa gaatttgaaa gagttccaaa caatgctgat
     1981 tttgctgttc ctattatagg cgattcaatg gaaccagtta tcaggaatgg acaatttgta
     2041 tttgttaagg aacagccaga cgtggaagac ggtgagattg ctatcgttga aatagatggt
     2101 gacggcgtga cgtgtaaaga agtatataag gattatgaga gtcaaacgat tattctacac
     2161 tctataaacg aattatatga agatagagtc gtctcaccgg aacagattag aataatcggg
     2221 aaagtcgtat tctaaaaaaa taccccagtc gaagttggcg ctccggctag gggttagtat
     2281 ttattattat agtagaaaga aggaaaagaa tatgccaaat tatgttgtat tgcaagttgt
     2341 attaaaagaa aagtttatag gaaaagggtc gcaaaaccta tcagaacttg aaaacactat
     2401 aaatagacag tgttctaaag gttatcgatt acacactatt tccactacaa acggtggtag
     2461 taaaggtttc ggtggtggcg atagaatcca agctacttta gttttcgaaa gtctaaatta
     2521 acaaaaaaca cgccctctcc cctaccaagt ttgagtgacg tgagatatta gacctatata
     2581 gtaggcttct ttacaactta ttgtatcaaa gaaaagagga gtataaaatg aaaaaagtta
     2641 gcgttatgtt gttgttaagt actgctctgc tactttcagc ttgttcaaat aataaaaaag
     2701 ctgaatcaac agatgccact tctaaccaag aaacaaaaat aagtaaaaca aaagaaacaa
     2761 ctgaaaccag ttcatctact agcaaatcta catctaaaac agattctagt ttaacagtta
     2821 caagctccaa ccaagttacg gcggagccta gcccaacagt tataagctcc agtcagagta
     2881 caatccaaac cgcacctcaa gaagaaacat atgaacagat gaaacaacgc actttacagt
     2941 caactccagc tgatcgtgca aattggtcca acaaagagtg ggaagctttc ggcgtggccc
     3001 tttatgaaaa tggattgact acagatgatg ctggcaatat tatcagtcaa gatcagaaag
     3061 aacaacaagc agcatctcaa caaaatccag aagaccaaca aacaagcgct cagcaagacg
     3121 ctgacacttt atcacttact gattttgtta acaaatacgg gatgtcgcct gttgcatgga
     3181 aagtacagaa tggaatgtct gaagaagaag cattgcgtac aacacagcaa aagacttccg
     3241 gtgaagttca attaggattt tctaaatacg gaattcaata atatattttt atgccctact
     3301 attttgccta taatctctaa aaaagttata aagaaaaaag cccgtgctgc aacacggact
     3361 aaaatctcgt ttctaagatc ctacatataa ataatatcat aggaatgagg agtaaaaaag
     3421 ttattagaga aagaaaatat aaaaagaaac aaaggaaaga gtaaaataat gaaaaagaaa
     3481 aaaggtacac aattatccgt tgattcacca aactggttat taattattac agttttatgt
     3541 tttgcagctt ttataatggt atcaccccaa gtatatcttc atagtgttat attagggaat
     3601 gatattatgt ttcattttaa taggttttat gaaacttata tgcagataaa aaccggtaat
     3661 tttaatttct ttcaatccct gtattctttt cagagttctg gcagaattat tactgctttt
     3721 tatggcgcag atttcgctta tttgcaaggg ctacttttaa taatcttaaa atcatggttt
     3781 aaatatcaac ttgtctcttc tttcagttgc ttttttatag ctggtgcttc gatgttttgg
     3841 ctaacttata agtgtaaagt acgaactaac ataggattaa tcattgcttt gttgtacatg
     3901 tcttcatcag cagtttcgta ttatcctatt gctcaagctt ttactggttg gggtgctgct
     3961 ttaatgccgc ttctttttgt gccagcaatt gaagcattaa aaaataaaga aagacctatt
     4021 cgtccattgc aacttgcaat ccctgtaact ttattactat ctactcattt actttcatta
     4081 gtaattggaa tattagctat tttacctttt tacgttattg cttttattaa tacaaaaaat
     4141 aaactgaaca tg
//
LOCUS       DAXWLF010000193         4083 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXWLF010000193 DAXWLF010000000
VERSION     DAXWLF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.197"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            52..1353
                     /locus_tag="ACGE4U_002513"
     CDS             52..1353
                     /locus_tag="ACGE4U_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8394613.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1380..1640
                     /locus_tag="ACGE4U_002514"
     CDS             1380..1640
                     /locus_tag="ACGE4U_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFF8394614.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2639..3874)
                     /locus_tag="ACGE4U_002515"
     CDS             complement(2639..3874)
                     /locus_tag="ACGE4U_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8394615.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1160 a          663 c          855 g         1405 t
ORIGIN      
        1 gcgttgtcat gcaatgaatg gttcatattt tattgaaaag gagtggtttt agtggaaaag
       61 ctgatgaaat gggtcgaaga gaaacttgtc ccaccaatgg caaaaattgg tacacagcgt
      121 catttattag caattcgtaa tggggttgtt tcaacattgt cattgatttt gattgggaca
      181 ttttttatgg tctttatcaa tttaccgttt cctggatgga atgaatttat tgccccttat
      241 tcggcaacga ttgttttacc ctttaggatg acgatgggat tgatggctat ttatgcaact
      301 tttgttatgg gttcggatct agctaaatca tatggattag atagtgtgac aggtggtata
      361 ttatctttag gaacgttctt tatgttacaa gttcctgtaa acgttttaac tcctgaagag
      421 gcacctcttg gctgggtctt acctatgagt tctttgggag catctgggat gtttgcgggg
      481 atcttatcta tgatttttgc agtggaagta tatcgtttct ttaaaaatcg aaatattact
      541 atcaaaatgc cagaacaagt gccacctgct gttgcccgta gttttgaggc attgattcca
      601 ggtgcagtca ttttaacaat tacttggctg attcgaagcg tcgcgggctt tgatgtaaac
      661 gctgccttat tatcgttatt cgagccttta accaatattt tagggaataa tttgcttggt
      721 gttttactac caatgttttt gatccatttg ttgtggtcat ttggtatcca tggcatgagt
      781 attgtagggg ctgttgttcg tccaatgtgg ctgatcatgt tggatgagaa tgcaaaggca
      841 ttggcagatg gtacagctgc cacccaatta ccctatatcg ctcctgaaca gttttatcaa
      901 tggacggtaa caattggtgg tgcaggagca acgatcgtgg tatctgtttt gtttatattc
      961 ttctgtaaat caaaattctt gaaagaggtt ggtcgttttt ctattattcc tggtatcttc
     1021 aatatcaatg agcccataat ttttggagct cctatgatgt tgaatcctta catgttcatc
     1081 ccgttcaact tagttccttt agttttgact attatcagtt attttgcagt caaattagaa
     1141 atggtcaata gatttactgt tttgccaaca tggacattac cagctccaat cggcggttat
     1201 ctttcagcag gaaatgactg gcgtgttgtt atcttgatca tgatcaacac attgatagca
     1261 tttattatct attatccatt tgtaaaagct tatgataaaa agatgctgtc agatgaacaa
     1321 agtcgtggag aaaacgaagt ccagactgtc taagtttggt ttttagaagg tgggaaacaa
     1381 tgtattggct gattttattt tttgttttta tctttttatt aacggctagt caccttatat
     1441 tgaatatgct agctacatat catattcaaa tcaatcgatg gatttgggca ctggcatcat
     1501 ttttgatcgt gattcttcca aagatcattg taccgcatat gaatgttttg ttttcatggg
     1561 ggacatatgt actttgtgga atttttgcta tcaattttat gatcgagcag catagatggt
     1621 ttgtcacatc gaagctttaa atgttctaaa agtggttgta acagaagtgg ttagcttcaa
     1681 gaaataagaa gaaatttacg aaaattgctt cttaaatttt tgtgaaatgt tggcttattt
     1741 ttcgtttggt tgaacttatt accgcagatg caagcttttg accaccgttt attcggaata
     1801 tgggactgtg acaaggcttt tgtcacagcc cttttttaca taaagattat tattgtttta
     1861 actgttttgg cagaaagaag gaggaaaaaa tggaaatttt atcagcaatg gatttagaat
     1921 tagtagatct actaccaaag tgatcaagtc aaatatagat ttgggagctt acaaccatat
     1981 agtaggggct gcagtatggt gtatgaatgg taatatgtat gatgtgagtg tttattcaaa
     2041 tcatgaaacg tgtatagaaa taattgtgct gggaatagca atcagtcatg gtgaacgtga
     2101 cttttagtgt atcgttgctg acgacataga acattctgac atgatctact caatatgtgg
     2161 ggattgctgg cagtttatat aggagtatac atcggattgc gagatcattg tctcaacaga
     2221 agagggaggt tgattatcaa agataaagtg gtaaatccta catttgattt agtaattgtt
     2281 gaggggacta gcggcacaaa tctctacaaa gcgatgcttc tagatctata tgacacattt
     2341 taagaaaatg tatttgccta attattatgg tctttctttt gaagaaacgt tgagatgatc
     2401 caaccagaga aaggtgtcaa agaatttgac taagaataaa tggcagacgc tacctatgct
     2461 catttggata gcgtcagatc ttttatttta aggatgattt tgtgtttttt agacaccgta
     2521 atttaagtaa ctctaacttt tcaatccact atttactttt tttgtatttt cactgtctgg
     2581 gtctcgaata gcgaaattac tgatgagtct ctattaaaaa agctttttct acttgaaact
     2641 atattgggtg gttgctcagg aaggtttcga tttcttgaat tgcttgttgt ggaccattta
     2701 ttggaaaaac ggcttgcagc gtgcgtgcct gctgacgaat cttgggtgaa gaaacttcgc
     2761 gcagagcaat ctgcagtttt ctttcagtca ctggttttgt gagggcgatt gcgtttccgt
     2821 attttacaca atagtcaata ttacagcttt gctcaaattg catccccaca ccgataaacg
     2881 gtttgccgga ttcacaggca gtttggacgg tcccttcccc gccgtggata aaagaaaaat
     2941 caatgaattc atgcaaaaga tgactagaca gatattctgt tattaaaaga tcttctgagt
     3001 cagattgaat gtcatcaagc agatagtcaa taggtgcaat aaccgctaga tccggttgtt
     3061 tttgtaagaa gaacaaaatt ttgcgtaaca tacgcggatt gcctgaacta cccattgcga
     3121 aatagataat acgttttctt tcttgttgct ttcgctgtat aaaagttagc acttcttcag
     3181 gcaaggaagt actgagctta gcaaaaattg gaccaacgat tcgataattt tccggcagct
     3241 gttcttcttc caaaaatagt ttaggcgttg tgatgagatt tagatcagca tccatcattt
     3301 gtaaacttgt ttttggtaaa ggaagcattt ctttttgtgc agcgtgtttg aaggcattgg
     3361 ggatccatcg ggtatttact atacgctttt ttgtccagtg ccaaagggga ttgcttaaag
     3421 ggccgaagaa acgtaactcg tggggcatca gcgcatgctc tgattgaaaa aaaggttcac
     3481 tcaaagcata gggtttaaca tatactaaag gaatcttttt attgcgggcc gataaaaaaa
     3541 ttgtcacatt cgaacctgtc acaatcagtc gaggctcaaa cttagcaatc agactctgtt
     3601 cactagtgat acgagaggca accatttctt tggtaaaagg gtttttaagt gtacgcaact
     3661 ggtcaaattt tatgatttgt tcttcttgtt ttttcgtaag gatcggcgct agtaaatgat
     3721 actcaaagcc actttctgtg atcaaaggag caaaagtttt agaaaagcca aagaatacac
     3781 agtaataacg gtctttcata gctttggcaa cctcaatcat tcgtgttgtc tcagctaaat
     3841 tgaatactgc aggagcaaaa agaattgtct tcatgaaatc ctccttattg gaatgagtct
     3901 atcccttcgt ttcttactag tatactaaaa aacacagcgt tcgagtagcc tatgtatatt
     3961 ttgaatatta aactgtctct cgctgttttc acaacttcta aatttaaatt ttgtaatcaa
     4021 gttgaaactc caattttcaa aaaaacacgc aacaattttt tgatcgattt atatgttgtg
     4081 cca
//
LOCUS       DAXWLF010000194         4066 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXWLF010000194 DAXWLF010000000
VERSION     DAXWLF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.198"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            54..506
                     /locus_tag="ACGE4U_002516"
     CDS             54..506
                     /locus_tag="ACGE4U_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394616.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="ACGE4U_002517"
     CDS             520..1209
                     /locus_tag="ACGE4U_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394617.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="ACGE4U_002518"
     CDS             1334..3685
                     /locus_tag="ACGE4U_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFF8394618.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3765..>4066
                     /locus_tag="ACGE4U_002519"
     CDS             3765..>4066
                     /locus_tag="ACGE4U_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394619.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKE"
BASE COUNT         1407 a          521 c          833 g         1305 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     3781 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     3841 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     3901 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     3961 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     4021 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaa
//
LOCUS       DAXWLF010000195         4040 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXWLF010000195 DAXWLF010000000
VERSION     DAXWLF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.199"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..804)
                     /locus_tag="ACGE4U_002520"
     CDS             complement(<1..804)
                     /locus_tag="ACGE4U_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFF8394620.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGL"
     gene            1139..2530
                     /locus_tag="ACGE4U_002521"
     CDS             1139..2530
                     /locus_tag="ACGE4U_002521"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFF8394621.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            2568..3398
                     /locus_tag="ACGE4U_002522"
     CDS             2568..3398
                     /locus_tag="ACGE4U_002522"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8394622.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            3370..>4040
                     /locus_tag="ACGE4U_002523"
     CDS             3370..>4040
                     /locus_tag="ACGE4U_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8394623.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSF"
BASE COUNT         1340 a          608 c          775 g         1317 t
ORIGIN      
        1 cagtccatta cgacgcagaa tcccgttaga attctcgttt aatgggcgtt gagaaggtgt
       61 tccagggtcc gcaaaatata tatcaatatc atgttggtta ctaatggctt tccagttaga
      121 aaattctttt ccacagtcaa acgtaataga tttaaagaag tttttaggga agcgagaaaa
      181 ccattgatta agggcagttt caatatctaa tgccttacgg ccgttgggtt taatcgtgat
      241 aatgacttta gataatcttt caactaaagt aatgacggca cttttatgat gaatgccaac
      301 gatcgtatca ccttcaaggt gaccaaactc agaattgaaa tcaggataat tatcaggtcg
      361 atcatggatt gagcgctgat actgttgttt tccccgtttt tcctgatggc cattgggttt
      421 tcttttacct ttcatcggta gtgtgtcaat atcaaatatt cctttagaaa ataaacgata
      481 aagtgttctc ataccgcatg aaacagacct ttcttttcgc ccgataatga cgtcaggcgt
      541 ccaaccaaga gtgactttct ctttaatgta atctacttca tgagcaggta attgaatgac
      601 ttttctacca cattttttct tattttcttt atactggtgc caataatcaa gagcagtctt
      661 gccttgcttg aacttattga ctacattata aatagtttgt atagcacgtc ccattcggtt
      721 agcgatttca accggcttat tatgttgaag ataatatgat tctatcattg tcagttcgtc
      781 tatggtaaga tgtttgtagg tcatttatgg ttacactcct ttgttttctt tcgtcggaaa
      841 tacaatttga gtgtaccata aatgcctttt tatttttcta acttaatttt acaattcgcg
      901 ttatatgaat attgtaactt gtgaataagg aattagcagg agaatactag ttcagcataa
      961 aggaagtgat gggacttctg agacaacttt ctgttatgca gaaatacaca tataaacaga
     1021 caataaacgc ttttccccgt ttcttttaat ttcctttaca atttggttat tcactaggtt
     1081 tttaaacttt gctatggtaa gatgatagag gcattatttt atggttagga gtgtatatat
     1141 gaataaaaat ggggagtgga atgcggcacg cagaatattc attattctta tggatgtact
     1201 tgtattaaat gtatcgatct atcttagttt tttgctgaaa ttccagggag aagtaccagt
     1261 gagaaactta gaaacctttc agcattcagc aatttttatt tccatcatct ttattgcgtt
     1321 gaatattttg ttaggagctt atgtattcta taaccgaatg atcagtgata ttgtcttcat
     1381 tactgttatt ggacaagtac taatagcatt ggggatcatg gtagttactt ttgctggccg
     1441 atggtttgca tttccgcgtg gcgtcatttt gatctcgttt atcctaggta cgatattact
     1501 tagtgtttac cggatagtcg tttacaaact gtacttaaga gtcagtcgtg ataagaaagt
     1561 ccttatcgtg ggtttggaga aagatgtcgc accagctatc caaaattttc aatctaagaa
     1621 aaacaataaa cataaggttg aagcagtggc aatcgataat tattacgaaa atacaaaaaa
     1681 attgatcgat gatatggata tcgtctattt ggcttctaaa atcccagaag atgaaaaaat
     1741 caacatttat caattagtga ctaaaaaaga gaagaaatta tttttaaata cgacttttga
     1801 gaatctgaca atgataaatc cgaatattat gaattttgaa gatgaaagca tcattgaagc
     1861 ttctgggttc agaatccaac cagaatacga actattcaag cgtctctttg atatcatcgt
     1921 ttcgcttatt ttactgatcc tcgcctcacc ttttatgcta atcacagcga ttttggtaaa
     1981 aacaacttca cctggaccaa tcatctataa acaagtacgt attacgaaaa atcaaaagga
     2041 attttcaatc tataaattcc gttccatgag tgcgacagct gaagcaaaat caggaccagt
     2101 cttggctaaa agtaacgatg cacgtgtgac accagtaggt aaatttatcc gtgctgtacg
     2161 ttttgacgaa ttacctcaaa tctttaatgt attgaaaggc gatatgtcga ttgtcggtcc
     2221 aaggccagaa agaccatttt tcgttgatca gttcaatgaa gaaaatcctt attattactt
     2281 aagacacaat gtacgtgcgg gaattaccgg atatgcacaa gtatacggaa aatatgtatc
     2341 agattataac agtaaactac gttttgattt gttgtatatc aaaaagtatt ccttactatt
     2401 agatttgaag attttacttc aaacaatcaa aatcttattt gataagatgt cctcacaagg
     2461 actagatgaa gatgaaaata tcagttcatt agcagaaagt gcatttccaa aagaaaaaac
     2521 gtatcgttag attggaaaaa ttttgagcaa agaagtagga gagaacattg gaaaataaaa
     2581 atataagtat actgctttct ttgtatgata aagaaaagcc ggaatattta aaggaagcat
     2641 tagctagtat ctttgagcaa acggtgatgc cggcagaaat cgtattggtt tatgatggcc
     2701 ctataaatga gaagcttcag gcagttgtca cttatttcca gcaaaaagtt cctcacttgt
     2761 ttacagttgt aaaattagaa aaaaatcaag gattggggat tgctttagca actggattag
     2821 aacatactaa aaatcgctta gtggccagaa tggatacaga tgacatcatg gaaccacaca
     2881 gattggaaaa acaattagag atcttccaag aatatccaga aatagcgatt gttgggagta
     2941 atattgaaga atttactggc acacttagca atgttatcgg aagaaggatt gtacctgagt
     3001 ataatgatga aatccgtgtg ttttcgaaaa gaagaaaccc ttttaatcat atgacagtca
     3061 tgtttgataa aaatgctatt ttagaagtgg gaaactatcg cccattaatt ggatttgaag
     3121 attattattt gtgggtaaga ctattgaagg cgggatataa aggatataat atccaagaga
     3181 ttctagtaca tgctagagca ggtgctgaca tgtatgctag acgtggagga agtaaatatc
     3241 ttttaccagg aattaaaggc cgatatcgcg tatggaaaga tggtctagga tcactaaaag
     3301 attttctgat agttatttgc ggacatcttg taattagttt attaccgaat aaaattaggg
     3361 gaaaagtata tgaatcaaaa cttagaaaaa caaactaaac tttctataat catacctttt
     3421 tacaatgcaa agaatacttt aagaagagct gtagacagcg tcttaacgca aggttatccc
     3481 aatattgaaa tcttgttaat tgatgatgga tctacagatg gtagtagtga cattggtgac
     3541 gaaattgcta atctagagag taaagtagca tgttatcact tggaaaacgc tggtccatca
     3601 gttgctagga actatgggat aaaagaagca actggaaatt tggtaggatt tttagatagt
     3661 gacgattatt ttttacctga ttgttttaat aatgttttga aagactacca taggaatcac
     3721 cccgatatca ttgcttttgg attaagtaaa ggaaaaaata tagaaaatgc aaatttattt
     3781 gttcccgaaa agaagattgg ttgttcaagt gaagaagcaa ttgagcaaat gtttagaagt
     3841 aaggcagtgg acttttacgt atggaataaa ttttttaaaa gggaattatt caatcatata
     3901 gtttttccag aaaataaatt atatgaggat atggttccta tgtatgaagc ttttagggcg
     3961 gctagtaaaa ttgatatttt gccattttca ggtatttttt attatcaaaa taacgatagc
     4021 atcgtttatc agtcttttaa
//
LOCUS       DAXWLF010000196         3964 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXWLF010000196 DAXWLF010000000
VERSION     DAXWLF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.200"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(73..1578)
                     /locus_tag="ACGE4U_002524"
     CDS             complement(73..1578)
                     /locus_tag="ACGE4U_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8394624.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1575..2261)
                     /locus_tag="ACGE4U_002525"
     CDS             complement(1575..2261)
                     /locus_tag="ACGE4U_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8394625.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2454..3875)
                     /gene="gndA"
                     /locus_tag="ACGE4U_002526"
     CDS             complement(2454..3875)
                     /gene="gndA"
                     /locus_tag="ACGE4U_002526"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFF8394626.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1091 a          844 c          682 g         1347 t
ORIGIN      
        1 aatttctcac ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt
       61 aaagcacatg ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt
      121 cccttgccct actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt
      181 agcgatagac aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg
      241 ataaaaacga ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg
      301 aatcgcgatc tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt
      361 agttgaatac tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg
      421 ataaatttgc agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg
      481 atataatatc ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc
      541 attcgcataa tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa
      601 gcttttcata cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg
      661 atcatctttc ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt
      721 ccttaactca tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat
      781 acggtcaagc atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat
      841 ttcaggcatg tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag
      901 taccttgagt ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat
      961 caaagaaaca acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa
     1021 agaagataat tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata
     1081 tataggctgg atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt
     1141 acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc
     1201 caaatcataa acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat
     1261 cttcattaag tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt
     1321 cgctttcgtc agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa
     1381 acgagaagct acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag
     1441 attgatagat gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa
     1501 agaactcgca aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga
     1561 ttttttcggc tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag
     1621 ctttcctccc cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg
     1681 tttgtttcaa cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat
     1741 accacgttca cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca
     1801 attacttcat ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa
     1861 gtaatagtag tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat
     1921 gcacgaagac gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct
     1981 ccgtggtcaa gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt
     2041 ggtgtatttt tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc
     2101 atcagatcca acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg
     2161 ccgttgtagt ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt
     2221 gccaagttct tttcatcttc aatgattaga atattgctca tttattattt tatcctttct
     2281 attctactgg ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag
     2341 tcattttatc atggatagca attttaaaaa agagctgaag tctactcata acaatcgact
     2401 tcagctcttt cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct
     2461 tcatggtacc atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg
     2521 ccaaagtaat cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa
     2581 tcgaagtaag cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag
     2641 gcaacgacgt ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct
     2701 agcaataaat tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat
     2761 cgagctcgga tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc
     2821 caatcaaatt cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt
     2881 ttactgaaat acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac
     2941 tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca
     3001 gaaatatagc gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc
     3061 gcgctttggc ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct
     3121 actactggtt tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct
     3181 atcaaatagc tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct
     3241 actgaaaggt ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct
     3301 ccatactcaa taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata
     3361 tatgtgacac atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca
     3421 actagcgcat aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct
     3481 tcttcaccac cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca
     3541 ttacgacgca tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta
     3601 tcaaggtgag gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt
     3661 agcaagatcc gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct
     3721 ttgaaatttt tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg
     3781 aataaagcca ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg
     3841 gccataccga caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt
     3901 tctccctaat ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
     3961 attt
//
LOCUS       DAXWLF010000197         3817 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXWLF010000197 DAXWLF010000000
VERSION     DAXWLF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.201"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..890)
                     /locus_tag="ACGE4U_002527"
     CDS             complement(<1..890)
                     /locus_tag="ACGE4U_002527"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8394627.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDS"
     gene            complement(1347..1775)
                     /locus_tag="ACGE4U_002528"
     CDS             complement(1347..1775)
                     /locus_tag="ACGE4U_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394628.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1803..2441)
                     /locus_tag="ACGE4U_002529"
     CDS             complement(1803..2441)
                     /locus_tag="ACGE4U_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8394629.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2445..>3817)
                     /locus_tag="ACGE4U_002530"
     CDS             complement(2445..>3817)
                     /locus_tag="ACGE4U_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFF8394630.1"
                     /translation="ISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKE
                     QFLYLFQMYVFLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVA
                     NVELATIVFSSSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQEL
                     YETLNKNGSILVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHP
                     VILIPERYRHTDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPV
                     VEILTLKNSMYLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQ
                     TEEIAVKVLSRSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFE
                     TYKDFFMMNLIKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQL
                     LLMNGG"
BASE COUNT         1160 a          726 c          564 g         1367 t
ORIGIN      
        1 gaatctgaag tgaatgtact caaaacattt tcaaagtctt tgtctttgat ttttacatta
       61 gcgtctttca atacttcgcc aattacttta ttctggaagg tactgtcact taattttgtg
      121 tctgttgcaa tttctttcaa ttcttttttg tatttatcca tgtcgttgcc tttgttttgg
      181 tttttcacca ttttaaccac ataatattcc gtagtataag ttgaagcatt ggtagaagtg
      241 atcacatcag aaacttgacc atctttcaac ttaaaggctg cttctttgac ttctgccgga
      301 accgttgtag tagtggaatc aaatttcact tttccgccat cttcttttgt cgtagtatca
      361 gttgacttgt ctttggctaa tttagagaaa tcgtctccct tatcagcagc tttcttcaca
      421 tcttttgcat catcttcact tgctacttgg ataatttgag cttccacttc tggatggaaa
      481 gattcccaag ccgtttttaa atcttcatcg gtcaatttga tatgtttctt tagaccttct
      541 tgaaaggcta gactttgttt gatactgtct ttgaatgttt gttctgtgta acctgcagat
      601 ttcaattgtg aatcaaatga atcacccaat tgtttctttg tttgatcata ttgagaatcg
      661 atttctttgg ttgatacgtc atctccatat ttatcttcaa atacttgata aacgatcaac
      721 tgttgcacag cggaatctgc tccttgcatt tttttcaagc tatccattac ttcttgttcg
      781 ctgattgtac cgcctttata actaacggct gttttttcac cggcattcgt acagcctgac
      841 aataagccag tgcctagtac gatggcaagt gctaaaaatc tttttttcat ttatcaattc
      901 tcctactttt cgttttttgc gaattgctaa aaacaaaggt gttcaagatg aaaatcatca
      961 agaacacctt tactaatctg ctcgatacgt cctgaataga tataaggcta cacaatccag
     1021 aaagttcgct cagcagaata aatgtcaagt agcacatcac accttaatat actacttctt
     1081 ttgttaccgt ttttcaagtc tatcttgcgt tccgtattta acgtaactaa ttcttagaac
     1141 aaatgttatc gattttgttt ttcttcttcc gctgctggga tattcaaatc agacactttt
     1201 atgttcgatt ttccagacat ttttttgttg tttttattat gatatatttt tatacaacta
     1261 aaaatgaata tattaaaact accaaaaata acttttaacg ctgtttcatt atatgttttt
     1321 tctattttat ataaactttt ggatacttaa taccaaccgg ttggattgta ccaacatgag
     1381 aatgtgtcag ttctatttcc tgtcgcactt gcataggccc atactccttt tgttgcattt
     1441 gctgatcgaa tagagtttcc aactttcgct tttgcaccgt gacgattata gacacgacta
     1501 tagtggttag aatgacctgt ttttctcgta caagttgtat cactataata aacccatctt
     1561 ccagagctat gattaattgc tgttctagaa atttcccggt ctactggtaa atttgactta
     1621 ataatattac ctttagcatc aaaatgaatc tctcctggct ttaaagattt gtaatagatt
     1681 tcaccgtgaa ctgaatttgc ttccacttca gatgacatag cgtttcctcc aacaaaacct
     1741 atccctagtg cacaaataat aactgctttt ttcataaaac gacatccttt cctttttgat
     1801 ttttaaagca tgatcttctt gtttcctatt ttggtcagat atggatcatg cgtgactacg
     1861 ataactaacg taccttggga agcttgttgc gataagattt caactacttt atccttattt
     1921 cttttatcca atgacccagt cggttcatcg gctaaaatga ttttgctatt cttcaataag
     1981 actctcgcac aggcgattct ttgcttctca ccaccagata atgtgtagat aggcgtactt
     2041 agcttcttgt ttatatttac ttttttcaat gcctcatact ttgctgttcg cttatctgaa
     2101 acatttaaat tcgaaaactt caatccaata tctaaattaa aatcgattgt ttcatattca
     2161 atcaacccaa aatcttgaaa aagaaaagat atggtatttc tttttatcaa gcgcttctct
     2221 ctcccagtta attgtttcgt ctcttgatcg aacaaataga attgtcctga actgaactga
     2281 tcattcaatc ctaatatatt caataacgta cttttacctg ctccactttc tccaacaaca
     2341 gtcacaagat ccccagcatc cgcagtgaaa cgatccaaat gaagaatcgt tttgtttcga
     2401 atgatttttt tcgcaccttt tatcactatc aacggctcca attgttaacc tccattcatc
     2461 agcaataact gacttttctt ttctgtagac aaaataaaaa caactgaaag aataaaatca
     2521 atgaagctaa agaaaaaaat attaaaaaga tatttaggct ctctttctat aagaaaaagg
     2581 aagattacac ttgtcgttcc ccatttgatg aggttcatca tgaagaaatc tttatacgtt
     2641 tcaaagagcg aataaccact tgaacgaaag atagcgattt tccgacaatt gtagacaaaa
     2701 tatatgcata acatgacata gctcaacaag aagaaagaaa agaaagtgag taaaaatatc
     2761 tgtatcaagt aatgaaatga tctgcttaac actttgacag ctatctcttc agtctgagta
     2821 tacggaaatg ataactgcaa gttatcacgt aactgacttt cttcaatcag ctcaaataac
     2881 ctttctttcg acaaagagcc tatcttgatc tttaacggtg gatagatttc tccgctgaga
     2941 atatttcttt ccaaatacat actgtttttc aatgtcagta tttcaacaac cggataatgt
     3001 tcgatccagg ggatagaagg aataaatgta taaatgggtt gattgttttt tataaataga
     3061 aatttcggtt ctttttcact gatttcttga taatattctg ttatttgcgc caggtcattt
     3121 tttaaatctg tatgtctata tcgttcaggg atcaatataa cagggtgcgt ttctttttcg
     3181 gttatttgga tcaaccttcc agagacatca atcaaaggaa atttcttaag ataattcaga
     3241 tttattttta tccctctgcc aaactgttca ttttcttcat tcaagtaatc tgatacattg
     3301 actaatatac ttccgttttt attcaaagtt tcatataatt cctgatctgc tgctgatgca
     3361 aaacgattgg aataaataaa atccacgtgg tttttcccga tggttactgg ataaaataca
     3421 tgatagtcct caccagcgat ttcttttggt ggttcatggg aaacagctaa ctgtgaagaa
     3481 gaaaaaacga tagttgctaa ttcgacattg gcaacggtca aaagaagaga aaacaacaat
     3541 ccaaatgtaa aaagaatgta ttggatatag ttgaacgctt ctcttaacga agcgattttt
     3601 tcaactaata aaagagaaat caaagagata ccatatattc cgctgaacaa gaaaacgtac
     3661 atctggaaca aataaagaaa ttgctcctta taatacataa aaataaaacc accaatgatt
     3721 cctgcaatta tgaatgtaat aatgattttc tttttcccca ataaacacca caatacttga
     3781 tgtaatgaaa agtcattatt caacaataaa ctgattt
//
LOCUS       DAXWLF010000198         3807 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXWLF010000198 DAXWLF010000000
VERSION     DAXWLF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.202"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(614..1153)
                     /locus_tag="ACGE4U_002531"
     CDS             complement(614..1153)
                     /locus_tag="ACGE4U_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394631.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            complement(1293..1973)
                     /locus_tag="ACGE4U_002532"
                     /pseudo
     CDS             complement(1293..1973)
                     /locus_tag="ACGE4U_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(2295..2516)
                     /locus_tag="ACGE4U_002533"
     CDS             complement(2295..2516)
                     /locus_tag="ACGE4U_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394632.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(2614..2859)
                     /locus_tag="ACGE4U_002534"
     CDS             complement(2614..2859)
                     /locus_tag="ACGE4U_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394633.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3099..3713
                     /locus_tag="ACGE4U_002535"
                     /pseudo
     CDS             3099..3713
                     /locus_tag="ACGE4U_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1223 a          674 c          566 g         1344 t
ORIGIN      
        1 agtttttttt gtcacataat tttgcaatca ataaatgttg cgcaataagt ataagaattc
       61 cattttctaa atttgaaagg tgatccataa tttcatgttc aagtagttta ttaattgtcc
      121 acttgtcgct tcatctaaaa attttatagg ttttaggaat gctattcgct tattttagga
      181 agattttaac tgtttgctat agatatatat catactagta tcgtaataaa agtactctaa
      241 aagaagaatt ttcaaatacg caaaattaag tacgaaagag ttgaaacaaa tttggagaaa
      301 aatgagcata gaatgtctga tgagttaaat agacctaaaa attatctaaa agtggagcaa
      361 aattaggaat tacaaacaaa acactaaatt agtttatata attttccgta atatttaata
      421 tatatgaaaa ggaaaacgta gaaaattacg tctacgtttt cctatttagt tctatatttt
      481 cattaaattt tatatttgct cttttcactt ttcggaataa attcactgtg actcttgata
      541 ccttctttta tatattgcta atgtattttc tccattgcta tgaaaataga atcaacatat
      601 ttataatatc tatctaatct attttcattt caccgcagca tcttattaaa taacgtgtaa
      661 ataagaatat aaaagttatt gaactaatta ataaagatag aatcatcgta gttggatacg
      721 tagatgaaac ctcaactata gttcttttcc ccgtaccttt aatagacact aaaccattgt
      781 cattttctat agtgctcttt ttcccattta ctttcacaac tgttccccaa taattgaata
      841 caggtaatgt gatattttgc tcatcggcat cttctactgt atagactatt ttggaattat
      901 atgctttatg agatgcattc tcccaccgat caccaacttt tacttcatgt ttctttaaat
      961 gatctttata tttcaatgca tctaatggtg catagtcata taggcctcca gttcttgagg
     1021 atgtataacg tgtaacatct tttttggtca taactaaacg acctttatac gactgaattt
     1081 tatgcgaata atttgcttct gttgctgcag tcacagtaaa aataataaga actattccta
     1141 caaggctcca catacttttt ttgttgcttg ttttccactt taaaaacact atggttaaga
     1201 ttaatgaacc aaaaagcact tgaaaaccca aggttctcgg ttctgttgcg aagtttgaaa
     1261 atcaaacgcg ccctctctga actccaaata tcttaggctg ttattcccat taataccttg
     1321 atttcagtag acaccgaaaa gccgaagagc gttccatttc ttcggttctt tttatatatt
     1381 cctcgaatgg tctccatgcc cttaatcgtg gaagaggctg tacggagact ttgataaaat
     1441 ttatttcgtc gtttaatagg tcgatggtct tgttctatta aattgttaag atacttcaca
     1501 gttcggtgct ctgtcttagt atataaaccc acactctgta actttctaaa ggcggagcca
     1561 agagaaggtg ctttatcggt cacaattgct ttcggctcac caaactgttt atggagtcgt
     1621 tttaagaaag cataggctgc ttgcgtatcc cgtttctttc gtaaccagat atctaaggtt
     1681 aagccgtccg catcaattgc acgataaaga taatgccaac gtcccttaat tttgatatag
     1741 gtttcgtcca ttttccatga atagaaggat tgtctatttt tcttcttcca aagataatag
     1801 aggactttgc tgtactctta tacccaacga taaatcgtag tatgacaaac atttattcca
     1861 cgatcatata acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca
     1921 acagcgacaa taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg
     1981 tcctctctgt cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac
     2041 agaaccgttt ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct
     2101 acctctcgag ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata
     2161 ctcatatatt tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct
     2221 gaaatttatt gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata
     2281 aaaagagcga accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga
     2341 atcacttcaa atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc
     2401 ttttctttgt aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct
     2461 ctttgttcaa ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt
     2521 gatccctcgc aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta
     2581 gatctaagta tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc
     2641 ttttgctttg cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt
     2701 aattacacac tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt
     2761 aattccatgg gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc
     2821 tatctcttga acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa
     2881 aagtaataat ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct
     2941 ttttcttcta ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag
     3001 tttaccctaa atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag
     3061 ggtgtattat aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag
     3121 tttcatccat cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg
     3181 aaaaaatttt tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg
     3241 aagctttaga ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg
     3301 gacgcaatta tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac
     3361 ttattatcac cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat
     3421 tcatcaaaga cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat
     3481 cgaaacgacg ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc
     3541 gtcctaagtt atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta
     3601 tcgttcagga tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga
     3661 ctaggcaaac aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt
     3721 tttgaatcac gtaattatta attagctttg tcatcaatac agacaatcat tttagattca
     3781 tctagctaag ttgattacat ttatgtt
//
LOCUS       DAXWLF010000199         3781 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXWLF010000199 DAXWLF010000000
VERSION     DAXWLF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.203"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            165..2606
                     /locus_tag="ACGE4U_002536"
     CDS             165..2606
                     /locus_tag="ACGE4U_002536"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFF8394634.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2936..3640)
                     /locus_tag="ACGE4U_002537"
     CDS             complement(2936..3640)
                     /locus_tag="ACGE4U_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394635.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1230 a          658 c          820 g         1073 t
ORIGIN      
        1 gataaaaaac ttactttact aaggtatcat aaaaagatta aaagaaatat aaaaaagaga
       61 atatttaagg attgttaaaa tttaatctta gaaaaaaata actggaacaa gagctatttg
      121 tttttacagc aaagaatggt atatttttgt aggaggtggc ttacttgaaa gaagtcgtta
      181 tgatagatgc tgcaagaacg ccgataggaa agtatcgcgg aagtttaagt ccatttactg
      241 cagttgaatt aggcacactt gtgactaaag gattattaga taaaacaaaa ctgaaaaaag
      301 ataaaatcga tcaagtaata tttggaaatg tcctgcaagc tggtaatggt caaaatgttg
      361 ccagacagat tgctttgaac agcggtctcc ctgtagatgt cccagcaatg acgatcaatg
      421 aagtttgcgg atctggtatg aaagcagtga tcttagcacg ccagctgatc caattaggtg
      481 aagcagaact tgtgatagcc ggcggaacag agagtatgtc tcaagcacct atgctgaaac
      541 agtatcagtc agaaacaaat gaatatggtg aaccaatttc cagtatggtc aacgacggat
      601 tgactgacgc attttcaaat gcacatatgg gattaaccgc agagaatgtt gcaacacaat
      661 tttctgtgag cagagaagag caggatcgct atgccttgtc gtcccagttg aaagcagcac
      721 atgctgtcga agccggtgta ttttctgagg agatcatccc agtcaagatt tctgatgaag
      781 acgtgttatc tgaggatgaa gcagttcgtg gaaatagtac attggaaaaa ctgggcacgt
      841 tacgtacagt attctcagaa gaaggaactg taacagcagg aaatgcttcc ccgttgaatg
      901 acggtgcctc tgtggtgatc cttgcatcca aagaatacgc agaaaataat aatctgcctt
      961 atttagcaac catcaaagaa gtagcggagg tcggtattga cccaagtatc atgggaatcg
     1021 ctccaatcaa agccattcaa aaattgacag atagatcagg aatgaattta tcaacaatcg
     1081 atctttttga gatcaatgaa gcatttgcag cttcttctat cgtagttagc caagaactgc
     1141 agttagatga agaaaaggtc aatatatatg gaggagcaat cgcattaggc catccaattg
     1201 gtgcaagcgg tgcacgtatc ttgactacat tagcttacgg attgcttcgt gaacagaaga
     1261 gatacggtat tgcttcatta tgtattggtg gaggtttagg cttggcagtc ttgttagaag
     1321 cgaatatgga gcaaacacat aaggatgtgc aaaaaaaaaa gttttatcag cttacgccat
     1381 ctgaacggcg cagtcaatta atagaaaaaa atgtattgac acaagaaact gcgttgattt
     1441 ttcaggaaca aacactttct gaagagctct ccgatcacat gatcgaaaac caagtgagtg
     1501 aagtggaaat cccaatgggg atcgcgcaga attttcaaat caatggcaag aaaaaatgga
     1561 ttccgatggc aacagaggaa ccatctgtca ttgcagctgc aagtaatggt gcaaaaatct
     1621 gtggaaatat ctgcgcagaa acacctcagc ggctgatgcg agggcaaatc gttctatcag
     1681 gaaaatcgga gtatcaagca gtgatcaatg cagttaatca tcgaaaagaa gagctgattc
     1741 tttgtgcaaa tgaaagttat ccttcaatcg tgaaacgcgg gggcggtgtt caagatatct
     1801 ctactcggga attcatgggg agtttccatg catacttatc tattgatttt ttagtggatg
     1861 taaaagatgc aatgggcgct aacatgatca actcgattct tgagagtgtg gcaaacaagt
     1921 tacgggagtg gttccctgaa gaagagattc tattcagtat tttaagtaat tttgctacag
     1981 aatcattggc ttctgcctgt tgtgaaatcc cattcgaacg cttagggaga aacaaagaaa
     2041 tcggtgaaca aattgccaaa aaaatccaac aggcagggga atatgcgaag cttgaccctt
     2101 accgtgcagc aacgcacaac aaagggatca tgaatggtat agaagctgta gtggcagcta
     2161 caggaaatga tacacgtgcg gttagcgctt cgatccacgc ctacgcggca agaaatggat
     2221 tataccaagg attaacagac tggcagatca aaggggataa actagttgga aaactaacgg
     2281 tgcctttggc tgttgccaca gtaggcggag cttccaatat cctgccaaaa gcaaaagcat
     2341 ctttagctat gttagatatt gattcagcca aggaactagc acaagtaatt gctgcagtag
     2401 ggttagctca aaacctggct gctttgcgtg cattagttac agaaggaatc caaaaaggcc
     2461 acatgggact tcaagctcgt tcattagcga tctcaattgg ggctattggt gaggaaatcg
     2521 agcaagttgc gaaaaaatta cgtgaagcag aaaagatgaa ccagcagaca gctatacaaa
     2581 tattggaaaa aatccgggaa aaataaaaaa gcaatatctt tagaagaata aagcaagatt
     2641 tacagttatt ttatttactg cagatcttgc ttttttctct atcagaaaat atgtttatta
     2701 ttagaatttt ttatagttaa ggttaggcat cttaagaaat caggcaactc caagatatct
     2761 tcaggtagaa gagaagtcgc ctaacattgt aggatcatag gtgatttgtt ggacaggtca
     2821 agctgatatt tgagcctgat gttagaagga atttcttttt acttgctgta gaaattatta
     2881 tatgactaaa aaggctggga ttttaaatcc cagcctttta tgacaaatag aatttttatc
     2941 gatcagtaat cgctgtcgct gcaaattgtt cgatcaaagg aatcaaagtc aatttgactt
     3001 tgtatttccg ttcgagtgct ttttcgataa cgaactcatt ttcagtagtc atagaaatag
     3061 ttgctaattt acgaggttca tcagcatttt tttcattaat aactacttca tgttcatttt
     3121 gttctgtgta aaatttagga tactcagcaa cggcttttga aaaatcagca aaacttaaag
     3181 cggcattcgt atcagttgag cgtgtatttt cagttgtgtc cgcttttggt ttttccgttt
     3241 tttcttctgg cacactgact gtttcttctg taactggtgt cgcaacagct tgcgcagttg
     3301 gttcatcgtt tttcaatcgt tgatcattcc agtaagacac gatttcaata agaagattgt
     3361 cacggaatcg ttcgaaaatc ccgttttctc ctgaacgaat tctctttaat acagtattga
     3421 agttgtcatc gattggcacg ttgatccgtg tatcttcatt tgttttccca gctaatccga
     3481 tcgttaagat ttgacggttt ttgggatcaa gcatgacttg taacggcaaa ccataacgtc
     3541 gcaagctctc ttcttctaga gaacggaata attgtcctct tacaccatcc aatgaaggac
     3601 ggaattgatt taataatttt tgtttctgtt ttttcttcat atgttctctc cttcgctcat
     3661 accctttcga ttataagaaa aaaacagcca aatgaaaagg agtaatgcaa aaaaacttgc
     3721 gtatacgcaa attacaacag aaaaatttgc gtattttccc gttttttgaa agaaaaataa
     3781 a
//
LOCUS       DAXWLF010000200         3776 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXWLF010000200 DAXWLF010000000
VERSION     DAXWLF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.204"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACGE4U_002538"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACGE4U_002538"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFF8394636.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACGE4U_002539"
     CDS             complement(1172..1867)
                     /locus_tag="ACGE4U_002539"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFF8394637.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACGE4U_002540"
     CDS             complement(1880..2596)
                     /locus_tag="ACGE4U_002540"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFF8394638.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="ACGE4U_002541"
     CDS             2926..3531
                     /locus_tag="ACGE4U_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFF8394639.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
BASE COUNT         1078 a          774 c          669 g         1255 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctc
//
LOCUS       DAXWLF010000201         3750 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXWLF010000201 DAXWLF010000000
VERSION     DAXWLF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.205"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..1756)
                     /locus_tag="ACGE4U_002542"
     CDS             complement(<1..1756)
                     /locus_tag="ACGE4U_002542"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8394640.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQGGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAACL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKVVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEA"
     gene            complement(1716..2414)
                     /locus_tag="ACGE4U_002543"
                     /pseudo
     CDS             complement(1716..2414)
                     /locus_tag="ACGE4U_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2502..3581
                     /locus_tag="ACGE4U_002544"
     CDS             2502..3581
                     /locus_tag="ACGE4U_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8394641.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         1119 a          824 c          717 g         1090 t
ORIGIN      
        1 gagcttcttt ttcatagacg aatttctcag atagtttagt tttctttatg agatttttgt
       61 atatttctga ccttcgatac aactcactat gagtaccttg atcaactact tttccttgat
      121 ccatcactac aattttattt accttttcaa tagacttcaa gcgatgcgat ataattagta
      181 ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat acgtttttca ttttccgcat
      241 ctaaactggc agtaatttca tctaaaatta aaattggcgc atttttcaaa aaagcccgtg
      301 ctatggatag gcgttgtcgc tctcctccag aaagtgaact tccgttttct ccgaccaatg
      361 tatgataacc gtcaggtagt ttttcaataa agtcttcgca gttagctaaa caagctgctt
      421 ttttcacttc ctcatctgac gcttgactat ttccaatacg tatattttcc aaaatacttg
      481 tattaaacaa agttacctct tgaaaaacaa tagcgatttt gctaaacaaa gaagctggag
      541 atacacgttt aatatcatac ccatcaatta aaatacagcc ttcatcataa tcatacagtc
      601 tagacactaa tttcaagata ctagttttcc cggaaccact agcacctact aacgcagtca
      661 cttctccttg tttagccgta aaagagatat gatctaaaat tggggtattg ttatcatatg
      721 agaaagagac atctcgcaac tcaatgtcaa aagatttcag aggactatct gatccccctt
      781 ggatactggt ttccttcata gctctaattc gctgtatttt aggagctaaa taaaatattt
      841 caagcactgc ttctttcata gaatcgaagg aatcctttat ttttattgcg gctagtaagt
      901 aaccaacgac gtagagtata gtcacttcac ccgttaacag taagtgaaca cctactagaa
      961 tgactactgc taaactaaca tatgagaaaa tagaggataa ggccatgact gaaaaagtac
     1021 ttaattctac ctttaaatga atcctttcac tctcttccat ttttttgaaa agtctgtctt
     1081 gaactttttt agatagatta aagctattaa tctcttgctg caattctata gtttcttgaa
     1141 attcttccga gttttctctc aaagtatcgt aatatttagt attggctttc gtttgggatt
     1201 tctttgataa caagattaag acaaaactaa ataacgttgg caaaataaca gctaatccca
     1261 ttttgacatt accaaccaaa agcatcactg aaataaaagg gaaaaacaga gccataccgc
     1321 cggctttagg tattgcatga ctcatcgcat gctcaatacc ttcaacgtca gacatgatag
     1381 tttgagataa atctgataaa ttatgttttg aaaaatagga taatggtaaa tttgacaaat
     1441 cgtctgcaat ttgcactctt aaatgcgcac tttctttata agtactatta tataagtttt
     1501 catactccct atctaacaac caatacatga aaagcaaagt aacaaacgat acggcaaaat
     1561 aaagccaacg gggctttgta ttttctaaaa ctaattcgtc tatcagcatc attataatga
     1621 atgctggtac catattaatg cagtatacaa aaaaactaga aatagaagct ttagttaaag
     1681 cttctgcgcc ctgatcactt aaagcaaatt tgtttttata aaaacttttc attgtcaagc
     1741 ctccaatcat ttgcattctc ataattagtg accaattgtt tatacacttg atgtttagcc
     1801 attaattcat tatgatttcc acgttctact attttccctt ctgacaagac aagaatttca
     1861 tctacagatt taatgctgga taatcgatga gctatcataa taactgtttt ttctttcatc
     1921 agatgttgaa aagccttttg tagttcatac tcattatctg gatcaataga tgcactggct
     1981 tcatccataa taacaatctt tgcatctttt aaaattgctc tggcaatagc tatacgttgt
     2041 ttttctcctc ctgacagata aactccttta ctaccaatta aggtatgctc tctttcggga
     2101 aatttttcta aaatcgattc gcaaccggct aatcttagcg cgtcaaatac tttacttgct
     2161 ggcgcttgag gatttgctat agaaacgtta tcgtaaatac tctgattgaa tagtttcgta
     2221 tcttgaaaga caaaagcaat tgtgttaata attgtgtctt ttgaatagtg atttaaaggc
     2281 ttaccaccaa ttttaatctg tcccttatcc acattgtaga aacctgaaat tagtttagct
     2341 atcgtactct ttccgcttcc tgattttcct actaatgcat aaattctttt ttgttggcgg
     2401 attttataat taacttagcc atttggaaat aaaaaaacgc cacgtgaatt ccatgttata
     2461 ttgaagttac cacaactctc aatagacaag gatgaattca catgacgcaa aaccatgata
     2521 acacagtttc acgcaaggga aaacacctaa cctactcaga acgttgccaa atcgccatcc
     2581 tgaaaaagga aaactattcc aaccgtcagg ttgcggatgt cctgggctgc gtgccgcaga
     2641 ccataaacaa cgaaatcaaa cgcgggaccg ttactcagct taaccgccag aatcagaacg
     2701 ggaaggtcta cgactattat catgacgtct acgacgccga tgccgggcag gcggcctacg
     2761 agaagaaccg cttgaactgc ggcagacggc ccttatgggc cgacacggac gccttcattg
     2821 aatgggcgga cgacatgatg ctgttggaaa aatggtcccc ggacgtggtg gtgggctttg
     2881 ccagaaaaca cgaattgttc gacccggcca ttatccctgg gaccaccacc ctctatcagt
     2941 ggatcgaccg tggcatcatg aaaacgaaga atatggacct gctagaaaaa ctgtcccgca
     3001 aaccgaaatc gtcccgtcat ggcaaccgcc cgaataaacg ggtgctaggg aaatccattg
     3061 aagagcgccc ggaggcggtt ggtacccgga aaaccttcgg ccattgggaa gtcgacacgg
     3121 tcgtaggaaa caagaccaag tcggatgccg tgctgctgac gttggtggaa cggcagacac
     3181 gcttcgaggt cattctgaag ctggccggga aggatgccga atcggtcaac caagcggtcc
     3241 gacagctgag agaacgggct ggtgcgtact tcggccagct gttcaagacg gttacctcgg
     3301 acaacggctc ggagttcgcg ggactgcacg atgccttgca ggatgtggca aacatctact
     3361 tcagccaccc ctatgcttcc tgggagcgcg ggacgagcga aaaccaacac aagctgattc
     3421 gccgtttcat ccccaagggc agcccaatcg gcaacgtgag cgaaaaccaa atcttgcgga
     3481 ttcagcgttg gatgaacgat tacccgcgaa agattttggg ctatgcaacg ccgcatgaca
     3541 cgttcgtcca ggcatttagg caggaacggt tgacggcttg acacgtgggc ccttgacaac
     3601 gagcctccct cggcatggct tgtgtctgga aagctggccg gggccagcca gtcgggagac
     3661 taccatgcct cagctcattg tcaagggccc actcgattag aaaaattaga cggttggtgg
     3721 taacgagcaa ccgggtcacc atgggtggct
//
LOCUS       DAXWLF010000202         3715 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXWLF010000202 DAXWLF010000000
VERSION     DAXWLF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.206"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            321..536
                     /locus_tag="ACGE4U_002545"
     CDS             321..536
                     /locus_tag="ACGE4U_002545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394642.1"
                     /translation="MIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYLVAPSCFIS
                     KQVHYTILEELTERQALKTIAKRCDFQ"
     gene            693..950
                     /locus_tag="ACGE4U_002546"
     CDS             693..950
                     /locus_tag="ACGE4U_002546"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8394643.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            1037..2275
                     /gene="istA"
                     /locus_tag="ACGE4U_002547"
     CDS             1037..2275
                     /gene="istA"
                     /locus_tag="ACGE4U_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFF8394644.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            2265..3005
                     /gene="istB"
                     /locus_tag="ACGE4U_002548"
     CDS             2265..3005
                     /gene="istB"
                     /locus_tag="ACGE4U_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFF8394645.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            3424..3579
                     /locus_tag="ACGE4U_002549"
                     /pseudo
     CDS             3424..3579
                     /locus_tag="ACGE4U_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1210 a          738 c          705 g         1062 t
ORIGIN      
        1 ctctataatt tctaggactg atagaatttt ctgtcggtcc tagatttttt gtaaaaaaaa
       61 acagacataa aaaaattccg atagaataga gtcgtctaaa acatcaatct gaaaggaagt
      121 ttttgatatc catttctcat tctatcaaag agctcttcac tattaaagat aaaaatattg
      181 aaattaattc ttttcaagaa actttttata aggacaagaa agccatcgta atcgaaggtc
      241 cattagttcg cacttttaga cattgtctat tttgtaaaga atctgcaaag cagatcgaga
      301 aaaacggcaa gaagatttca atgattatct taaatcgcag tgggaataaa caaacctatt
      361 tgcgtcttag aaaacaacat taccactgcc aatcatgcgg gaaatatttt actgctcata
      421 cgtatcttgt cgctccttca tgttttattt cgaaacaagt tcattacacg atattagaag
      481 agttgaccga gcgtcaagca ttaaagacaa ttgcgaaacg ttgtgatttt cagtaacgac
      541 agttcaacgg acattatctg ctctatcggc aacgctgcga cagaagctag actatttacc
      601 tcattgttta ctattcgacg agtttcgctc tctttcaatt ttccatggga agttctcctt
      661 ttcttgtatg aattgagaga cagggaagct ctttgacatt ttgtccagcc ggagaaaaaa
      721 aagatttgat tgcttatttc atgcgtttta caagaaaagc tcatatgggg gttcgataca
      781 ttgtcacaga tatgaatgcc ccttattttt cattagtcaa agaacccttc ccaaatgcac
      841 aaatcatcat tgattgcttt cacattgggc aacacttgaa caagtttttc gatcgtgttc
      901 gaaaacgtgt gatgaagcag ttgaatcaaa aatgtcaacg gcacctttaa aatgtaggaa
      961 aacggcaatt taaaattgtt ggtttaccta catttttgta tgctcttctt atcccaaatg
     1021 aaatggaggc tcagggatga gaaaagacat acgcgaagga gtgatgatct acgtgatcaa
     1081 tgaaatcaaa cccaattatg ctgcactagc aaaacaatac gactgcgact accgaacagt
     1141 taagcatgct tacgaagaag cccaagttaa agaatcaaaa cctccagaac gtaagaagcg
     1201 tccaagtaag ttagatccct acagagaaat catccaagac aaaatcaaag accaatgcag
     1261 tgcttacagc attttcaagt ttattgagca caaaggattt ggaggaagct acagtttagt
     1321 aaagacctat tgtcgccaga ttaagcaggc gaaagtacac aaggcaaccg tccgagtcga
     1381 acatagccct gggctttctg ctcaggttga ctggaaggaa gatatgcgac taatcagtaa
     1441 tcagggcgaa atttttgagt tcaatatctt tttgtacgtc ttgccatatt ccaagaaaaa
     1501 atatgtcaca ctaactttcg accgaaaaca agacacactc tttgagtgct tagatgatgc
     1561 gttttatcaa accggtggcg ttcccaaaga aatctggttc gataatatga agacagtcgt
     1621 tgaccattct cgtagtcagt tttccaaacc gcatttcaac aaacgttttt atgctttcag
     1681 taaggatgcc ggcttcacac caattgcctg tcggccattt cgtccgcaaa caaagggctg
     1741 tgtcgaagct ttagccagaa ctgttgaccg cctacgggtc tataatcatg aattttatga
     1801 tgccgttgag ctgtgtcatt tagtgaataa cctttgtgtt gagttaaata acgaggtttc
     1861 tcaatcaact gatcaaacac cagatgaacg ttggcagacg aacgaaaaag agcacctgca
     1921 tccataccaa aaaaaccttt tgaatcccta ttttgaagac tttattcgcc gtaaagtcac
     1981 caaggaagaa atggtcattt tccgtaaatg caggtactct gtggcgcctc gctatatcgg
     2041 taaagaagtc gaaatcgaac tctccgataa agaagaccaa gtacaaatct attataacgg
     2101 agaactgatt cggtcgcatc cattaacgac aaaacagctg aactaccacg aggaagacta
     2161 tttccaaatc ttgaaatccg atgtcatgag tcacaaagaa gacgatggaa tcagagcgta
     2221 tactcgtgaa agtctcaagc aatatgatct ggtggaggtt gccgatgaag aatagccatt
     2281 accaagaact gatgaccaac ttagagactc ttggtttgaa tcatatgcgg gaatatgttc
     2341 ctaactacat cgaagtggcc aatcgagagg agctttcttt aacggaagct cttctcgagc
     2401 tgacacgcag agaagttgat catcaaaata agcaaaaaat gaatcgtgtg gtggcacgcg
     2461 cccgcttttc caagagaacc agcctggaag agtttgattt tgccttccag ccaagtatca
     2521 ataagcgcga agtgctagac ttaaggcaca tgggctttgt ggaaaaacat gagaacttgg
     2581 tttttatcgg cagtcctggt gtaggcaaga cacatctgac gattgcattg ggaatcgaag
     2641 cctgtcgtca aggctatcgg acactattca tcacctgtca tgagctgctt ttgcgtctgc
     2701 gaacagccta tgaaaaaggt accctagagc gtgttctcaa acgatacgct cgctatgacg
     2761 tcttgattat tgatgagatt ggctatcttc cgattcaaaa acaggaagct gatttattct
     2821 ttcagcttct tgccatgcgc tatgaacacc gctctacgat cattacgacg aacattgtct
     2881 tatctcgttg gggggagttg ttccaaagtt ctgagatcgc agctgccatc cttgaccgtt
     2941 tagtacatca cgtcaaggtg ttcaagatca caggcaagtc ttatcgtatg agagacaaga
     3001 tctaaagtgt cgattgtcga caattactat tgccgaaaac ctacattttt atcctgccca
     3061 aaacctacaa ttttaagctg cccttgacat caaaaagaca cttcacaagc taagtgctat
     3121 cgacaattga aacctttgta taaattactt ctataatcag atgacgagtt ggattataca
     3181 acattcaaga aatggcagaa ttttcaatgg cgctatttaa cagaaagtga agtagttgat
     3241 tgtctaccct caatctctta tgaacttaaa atggcttagc agtattatca aaagctgctt
     3301 aacaattatc acaagaaaca gctagctact tcgagaaatg accaaagaag ttccctcgaa
     3361 acttcactat cttaaaaagc ctttttcata tctgaaaaag gcttttaact agcattttcg
     3421 aaaaagtatt ttaatacaaa attagagaat cttcgcacac atatcaaaac attgaaacga
     3481 tttgcttatg gattttgcag ttttaccaat atgcgcttgt ggatttttct aatcaacggg
     3541 ttaataagct atgcattaaa agctagtaaa tcttactaac tttgttgatc ataaatgacc
     3601 ttatcagccc gatttgacaa agaactactt ttttacaaca ttattttatt ttcgaataat
     3661 ctttcttact tcatttcgta tatctggaaa aagaaaataa acaaacggaa gataa
//
LOCUS       DAXWLF010000203         3714 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXWLF010000203 DAXWLF010000000
VERSION     DAXWLF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.207"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACGE4U_002550"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACGE4U_002550"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFF8394646.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACGE4U_002551"
     CDS             complement(2262..2513)
                     /locus_tag="ACGE4U_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFF8394647.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACGE4U_002552"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACGE4U_002552"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFF8394648.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACGE4U_002553"
     CDS             3463..3681
                     /locus_tag="ACGE4U_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394649.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1204 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caag
//
LOCUS       DAXWLF010000204         3644 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXWLF010000204 DAXWLF010000000
VERSION     DAXWLF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.208"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..1090)
                     /locus_tag="ACGE4U_002554"
     CDS             complement(<1..1090)
                     /locus_tag="ACGE4U_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFF8394650.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQ
                     MLAFSTVTREGYREYKSNPKECVNCPLLN"
     gene            complement(1062..2261)
                     /locus_tag="ACGE4U_002555"
     CDS             complement(1062..2261)
                     /locus_tag="ACGE4U_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFF8394651.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(2391..>3644)
                     /locus_tag="ACGE4U_002556"
     CDS             complement(2391..>3644)
                     /locus_tag="ACGE4U_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8394652.1"
                     /translation="RASKETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQT
                     DSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFV
                     SEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVE
                     VEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKN
                     LDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSL
                     LLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGN
                     NILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLV
                     LLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT         1095 a          712 c          585 g         1252 t
ORIGIN      
        1 ggtttagcag aggacagttc acacattctt ttggattgga tttgtattct cggtaccctt
       61 ctcgagtaac cgttgaaaaa gcgagcattt gatcttgcgg acaaatataa caatcaaaat
      121 actcgtcata gagaaactca ttttttgata atagcccctt tttccctttg ggccttgaat
      181 aaggcaaaca aggtcgaagg ttcaaatgag tcaaaaagtg aacaaactta ggtgtcttat
      241 acccagcatc agcgacaaca caattgattt caggaaaaag tctcttcgat tgtttgacga
      301 gctgaaaggc gacttgactg tcatgaatat ttccaggagt gatgattgtg ctaagaataa
      361 atccattgtc gtcgcatgaa gtatgggcag aataagcaaa ttgtttttct cgttcgcctt
      421 tgacgtagta gccgctttcc ggatcagctt tacttatttt tctttctttc agcttcactt
      481 ccttttctaa gtccaaagta aaagggcgtt ttccagcctc tattcgttga cgattgatct
      541 catcttctaa ttcatcctga taggctttcg cttcatcatg ggtcatttct ttcgtaaact
      601 tatgtttatt ggcgttagct ttgatgtggg tagaatccag atacaaatta tcctctgtta
      661 caaaacctgc tttaactgct tgttctaaaa tataggcaaa aatatcctca aacaccgtcg
      721 tttctctaaa tcggcgaaca taattctttc caaaagttga gaaatggggg attttctcct
      781 cgaaggagta gcctaaaaac cagcggtaag cgacattggt atcgacttcc ttaatggttt
      841 gtcgcatgga acggataccg aataaatact gaataaacac caatttgatc agaatcactg
      901 gatcgatact tgggcgacct aaatcggagt aagtggcttc gacgattgga taaataaatt
      961 caaaatcaag cgcggcatca atctttcgaa cgagatgatc ttgaggaact agatcatcaa
     1021 tcgtaagcat cgcaaattga tttctaccat tcgtattgtt tttagacatc atagctatca
     1081 ctccttgcat tcgttttcat aaatctatta taaaagaaaa gactgtagac atcatccata
     1141 aaatggactt tgtctacagt ctgcgcattc tcattagaga acactccctt ttttgtgact
     1201 tttctgaagc tagagtgaat actttcaact gcattagtag tgtacatgat ttttctaatg
     1261 gcgctgccat aatcaaagag ttgttcaaca tgagaaaagt ttcgtttgca tacatctact
     1321 gcaccagaat aatgagacca ccgattttga aaactgtcga aggcagcgtg tgcagcgttt
     1381 aaagaagaag cactgtagaa ttttttcatg tctcgacaga cttccttata atccttactt
     1441 gggaggtatc gtaacgcatt tcgaacaaga tgaacaatac agcgctgcac aatgacagaa
     1501 ggaaaaattg cttttgcgcc ttctttgaga ccggaaacac cgtccatcga aatgaaaaaa
     1561 acatcttcta caccacgtac tttcagttca tcaaatactt gcatccagcg atttttagat
     1621 tctgtttgat tgagccatag gcctaaaatc tccttgtttc ctttaagatc atagccaaga
     1681 atggtgtaca cagcatattc tttagcttca tagttttcgc gtaaagtgac gtacatacaa
     1741 tcaacgaata gaaatgcata acacttggct aatggccgag cttgccattc ttccaattca
     1801 ggaaggacgg catccgtgat atcagaaatc atttcgtggg aaatatcaaa gccgtaaata
     1861 tcttcaacgg ttgcggcgat atcgcattga ctcattcctc gtgcatacat ggaaagaacc
     1921 ttaccttcga tgtcggagac atctcgtttt ctattaggaa tcaactcagg ttcaaaagaa
     1981 gcctcccggt ctctgggaac atcaatgttt actttaccaa agctcgtttt aagcgtctta
     2041 ttgccataac cgtttcgtcg attgtcatgt ttctttggct ctttagaatg ggcgtcatac
     2101 cctaaatggt tattcaattc tccttgaagc attttttcaa ataagggacc aaacacatct
     2161 tttaaaacat cttgcatatc ctctacagat tcgggttgat aagtattcaa aatggattca
     2221 gctaacttct cagcatcagg atttcttttc tttctagcca tcgtgtaatc acctcttgat
     2281 cttattttag aaaaataaaa accgtgaagc aaccacccgc ctaggattga tggttactta
     2341 cacggtttat attacactct catttttggt gctaaaaagc accgaaaatt ttaattattg
     2401 tcaattaata attcttttgg attccttctt aatatattga atgaagaaat aatcaatgat
     2461 acaagaagca ctaaactcat aaatataact acataaatca tgaatttagg taatacattg
     2521 atatctaaac ttgataatgt cttgttaaat ccttcagctt ctgccccacc acctaattgg
     2581 ctagatgcag attgtttagc tatttgttta gctatatcac cagtaacctt attcaatata
     2641 ttattcccaa gcttgtcagc tgtatattga gctaagaaat aggaaccaac gaatgcgggg
     2701 attgatataa ataccatctc aattaggaat tgaccaaaaa tttctaattt tgatatacct
     2761 aatgatagta atactgctat ttctttcttt ctagcattca tccataagaa taataataat
     2821 gaaactacaa ctcctgcaaa tattaatgag ccaaaaaata atttatttga aattgagtaa
     2881 atacctgata tagattgttg taatgcaggg taatttgatg aacttttaat caaattatat
     2941 tctctccaat ttatatctaa tttcccaaga tcttttatta cactatcaag attcttatca
     3001 ccttttacaa agaatgttgc atcttggtat acagctgtat cttcggtatt accataaact
     3061 ttagcagctg tatgaatatc agtgattaat gtattttcgt atagttcttg tgctgcactt
     3121 actccaccgc tattatgccc atcgaataga cctttaattt ctacttctac tgtttcatct
     3181 gcacctttct cattatcagc atcaaataaa ttagatttta tttttatctt atctccaact
     3241 ttaaggttat ttttttcagc taaatcttta tgcattaaga ttttattttt atcttcattt
     3301 tctaaatgct ttccttctac taatttatat gcttctgata caaattttgt ttcttttgtt
     3361 gagtcgttaa ccccagttaa cataactgtt cttttaaaat tctttgctct ttcaggtgat
     3421 tgattcgcaa gagtctcttg agtttcaata atatcataat caactaaatc tgcaacactg
     3481 tttattcttt ttacatagga gtctatacta tctgtttgag atatcttttt aatatcttca
     3541 ccttttacat ttcccccacc tctaggtgtt cctggattta cttgtctatt tatctccata
     3601 gaaaaactat ttgttatatt agcaaatgtt tcttttgaag ctct
//
LOCUS       DAXWLF010000205         3632 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXWLF010000205 DAXWLF010000000
VERSION     DAXWLF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.209"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(73..1008)
                     /gene="murB"
                     /locus_tag="ACGE4U_002557"
     CDS             complement(73..1008)
                     /gene="murB"
                     /locus_tag="ACGE4U_002557"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFF8394653.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1081..2001)
                     /locus_tag="ACGE4U_002558"
     CDS             complement(1081..2001)
                     /locus_tag="ACGE4U_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8394654.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2114..2866
                     /locus_tag="ACGE4U_002559"
     CDS             2114..2866
                     /locus_tag="ACGE4U_002559"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFF8394655.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT         1050 a          726 c          638 g         1218 t
ORIGIN      
        1 cgtaaacaac cacaaaaaag agcaggacgg ccaccgtcct gctcttttac ttattttaag
       61 ttccattact tattaattgg ctaaacttct gcttgtttct gatattgctt gtttttctcc
      121 aatgatccgc acttctgttt ctaaagacac atcaaatttt tctttgatta cttcttggat
      181 gtgcgcaatc aattcaatat aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc
      241 agcatgtttt tcagagactt gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg
      301 gatcagctga cctgtatagt gtcctactgg gcgcttaaag acacttccgc atgaagggta
      361 ttctaatggc tgttttgatt gacgcaattc ggtcaactca tccattcttg ctttgattgc
      421 ttcataatcc cctgtttgca aactgaatcg agcttccaat acgatagcac gtaattcttg
      481 gactttactg tgacgataag aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc
      541 atcttcaagc aaaagatcca cttcagcaaa aacatctttg atctctccgc catacgcacc
      601 tgcattcata taaaccgctc caccgacact tcccgggatg ccgcaagcaa attcaaatcc
      661 agtcaatgat tctgccaaag ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc
      721 aatgaccatc gttcctttga catgaatctg tttcatttca gtcagcatga tgactacacc
      781 gcgaatcccg ccatcttgaa cgatcagatt gcttgcattt cctaatacca tccaaggtat
      841 atcatggatt cggcaataat cgatgatttg ctttacttct tcttgttttt tcgggaatgc
      901 aaggacatca gctggtccac ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa
      961 caatatagta atcgtggcta agctttcaac tatttcttct ttattcactg aggattttcc
     1021 tttcttttct caaacgtcct cattattcta tcacgcttaa tacaatcttg ccagtcttgt
     1081 tcaaagttga tgacggtagt gatctttaag cacttcctca cataattcat gacttcttac
     1141 aacttctttt tgtttaagca attctgtttc cgatttacct aaacggatct gagaaaagaa
     1201 ggcctctacc attggctcaa acccgcgttt gactagcgtg tttgtccagt cgccaaattc
     1261 tttcactttt tttccatcag catcttgaat gacaagctgg gtcagatcgt ttacttcata
     1321 agtaccattt ttacttgtta cttgatattt ttctatatta gcgccacttg ctaaatccat
     1381 cgttaaaata gccgtttgat tttgtgtttc taattgtaaa atggcatatt ccaaaaattc
     1441 ctttgtttct cttatctggt attttgttct caaaatgggt tcatctaata aatacacagc
     1501 tgtatcaata agatgtaaaa acaaatcata aaggacgaac tctgtcgttt cttttgccgc
     1561 tattcgattt ttttgcaggt ggatcaaccg cttatctggg atacgtttca attcttctac
     1621 cataggagca aagcgccggt tgaaaccgat catcaaaatt atttggttct tatcagctaa
     1681 ttcttgcaat tcacgaactt catttagatg gacactcagt ggtttgtcaa tataaacatg
     1741 gataccattt tctagacatt ttttggctaa ttcataatgt acttttgttg ctgcgtggat
     1801 catacatgct tcgatttttt cttctaaaag ctcatcgatc gtttcatata gatgttggaa
     1861 cccgtactct ttttgaattc tttcttttgt ttctttattt cttgttgcaa aataaaaatc
     1921 aagtgttcct tgttttttcg cataagttgg taaatatgct ttttctgcga tattccctac
     1981 accgattaca ccaatcttca tctactttcc tcctggatca tttagttact ttcatcttac
     2041 accatttttt actaaaatga ggtacaatag tatcgcaaag gtaaggaaat taattcgaag
     2101 gagtgtgctt ttattgaaat ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa
     2161 taaaaatttt ttagaagttt ttcatgagtt agacgccgat tttttctgtc tgcaggaaac
     2221 gaaactccaa gcaggacaaa ttgatttaga tcttccaggt tattatcaat attggaatta
     2281 cgcggaacga aaaggctatt ccggcacagc gatttttgct aaaaaaccag cattgaatgc
     2341 cacttacgga atgggtatcg atatccatga tacagaaggc cgtctgatta ctttggaata
     2401 ttccgatttc tttcttgtaa cctgttacac gccaaattct caaagcgaat taaaacgttt
     2461 agattaccga ttagaatggg aagaagcctt ttataattat ttggaaaatc taaaaaaaca
     2521 aaaaccagtc atcgtgtgtg gggatctaaa tgtcgctcat caaaagattg atttgaaaaa
     2581 ctggaaaacg aatcaaaaaa atgctggatt cactccagaa gaaagagcgg ctctttctcg
     2641 actgttggat aacgggttta tcgatacatt ccgttatttt tatccaaccc aggaaggtgt
     2701 ctactcttgg tggaactacc gattcaattc aaggaaaaac aatgctggtt ggcggatcga
     2761 ttactttttg acgagcaaag atttagagcc aggactagct gatgcaaaga tccatacgga
     2821 cattatgggc agtgatcatt gtccagtaga attagatttg aaataaagag gttgtgaaaa
     2881 agtcgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca cattttgcat
     2941 aaatttggca taataccgag aaactggctt ttgaacactg tttatcgaga gattgtgaaa
     3001 aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc atatttttcg
     3061 tttggttggg cttattaccg cagatacaag cttttgaaca ccgtttatta agaggttgtg
     3121 aaaaagctgc actgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt
     3181 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta ttaagaggtt
     3241 gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
     3301 ttttcgtttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattgagaa
     3361 gttgtgaaat cagcgtcctg acctgagtat tagagaaaca aatggcaaaa tagttcctca
     3421 tattttgcta ttttttgagc taataccgag ggtcagctga ttgaacaccg tttagtggag
     3481 aagttgtgaa aaagtcgcct actttcgagt cgtaagaaat ttttttgaaa atggttcctc
     3541 acaactttgc ataaatttga cttaataccg agaagtcatg atccccatct aactccaaat
     3601 cacaaggaat gagttcaaaa actagctttt ct
//
LOCUS       DAXWLF010000206         3552 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXWLF010000206 DAXWLF010000000
VERSION     DAXWLF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.210"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            75..1790
                     /locus_tag="ACGE4U_002560"
     CDS             75..1790
                     /locus_tag="ACGE4U_002560"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8394656.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1793..>3552
                     /locus_tag="ACGE4U_002561"
     CDS             1793..>3552
                     /locus_tag="ACGE4U_002561"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8394657.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTA"
BASE COUNT         1057 a          705 c          716 g         1074 t
ORIGIN      
        1 ctaaatttcc taagctacaa tgtggtagta cttgtttttt caattgtgga caaataaata
       61 ataggaggaa tcatatgatt cagttattac gttatgcaaa agactatcgt aagcaaatca
      121 ttttgggccc gttcttcaaa tttcttgaag ctgtcttcga acttgtcctg cctttaatga
      181 tggcttcctt gatcgataat ggactaaaaa tgaatgaccg aggaaaaatc attgaaatgg
      241 gcttatggat ggtcgctatg tctgtgattg gtctgatttg tgctatcatt tgccaatact
      301 atgcttcagt agcttcacaa ggatttggta cagagttacg taatcagcta atcaaaaaaa
      361 tcaatacttt ctctcacaac gagttgaatc attttggaac agataccttg atcacccgaa
      421 tgacaaacga catcaatcag cttcagctag ctttagcaat ggtcattcgt ttattgatca
      481 gagcaccgtt tttaagtatc gggtcagtgg tcatggcttt tgtgatcgac tgggaagtag
      541 gtctgttttt cttagcatta ttgcctattt tctcaatcat tttatttttt atcatcaaaa
      601 aaaccgttcc tctctaccaa aaagtgcaag aaaaactaga tcagctcaat gaacacgtta
      661 gtcagaatct tagcggtgtt cgtgtcatcc gcgcatttgc gcagacaaaa aaagaaacag
      721 agaagttcga taaaagcaca aatgatctag cgaacaatta tttacgtgtt tccaatatct
      781 ctgccctatt atcacctgct acaaccttga tcatgaatgt cgggatcatc tgtttgctga
      841 cagttggcgg gatcaaagta aatattggta gcttgcaaca aggacaagtt cttgctttga
      901 tcaattatat gaatcaaatg cttttggcac tgatcgttgt ttcaaacctc gtcgtgatct
      961 tcacacgtgc cgaagcttca ggaaatcggg tgaaagaggt gctagacaca gaaaattcga
     1021 ttcttgaagc tgaggtcagt actactcccg atttttcttc tgaagcaatt atcgcttttg
     1081 accatgttga cttccgttat actcctgatt ctggtctttc tctccaaaat attacatttc
     1141 agctgaagcg aaatacagtc cttggaatca ttggtccaac cggaagcggg aaaacgactt
     1201 tgacacaatt gattccgcgt ttctatgatg tcagttccgg tacagttata atggacggaa
     1261 tggatgttcg ttcttggcag ctggatcatc tccgtcagca aattgctctt gttccccaaa
     1321 cttctgtact atttaccggg actattcgtg aaaaccttca atggggtaag aaagacgcca
     1381 cagaaaaaga atgttgggaa gctctgaaaa tcgctcaggc agaagaattt gtccgtcagc
     1441 ttccaaatgg tttagataca aaagtcatgg aaaatgggaa aaatttctct ggcggacaaa
     1501 aacagcgact gacgattgca agagctttga ttgccaagcc agcattactg attttagatg
     1561 actctttaag tgcgttggat tatcaaacag acttggattt gcgtaaggcg ctacagactt
     1621 atcttggcag tacagtgatt attatctccc aacgtgtccg ctctattcaa gaagcacatc
     1681 atatcttagt gatggatcaa ggtaagattg ctgctcaggg tacccatgaa gaattacttg
     1741 ctcaatcagc agaatatcag gaaatcgtcg catcacagga ggaagaatag ttatgaaagt
     1801 aaaaaaatta tccttgcaga cgctccgccg attcattcct taccttactg cctatgcaaa
     1861 agacatttgg ctagctgtga tactaggaat catcaatggg attgcaacag ttgtgatgac
     1921 gttacaaatt ggtcaaagta tcgatcaaat gattggagaa ggacaggtcc gcttcaatcg
     1981 actgttccat ttacttattc tattcggtgg tatcgtttta atcaacgtag tcagtcaatg
     2041 gctcatccaa gtgttaagca atcggttagc ttacttatcc gttgcaaaat taagaaaaga
     2101 tgcatttgct catctgaatc gattgccact tcgttattat gatcaaaact cccacggaaa
     2161 tatcgtcagt cgatttacaa atgatatgga caatatttcc attgcaattt ctgcggcctt
     2221 caatcaatta ttttctggag tggcaatcat cattctttcg tttattttta tgatgcgtct
     2281 aagtccactt ctgacattgg tcgttctttg ttctacacca attattttcc ttgtgagttg
     2341 gatcgttgct cgtgcttcac aaacaaattt cgatagacag caaaggatta ttggggatat
     2401 ctcgggcttt gtgaccgaac gaatcggtaa ccagaaaatc gtgaaagctt ttcagcaaga
     2461 agaagctagt caacagcaat ttgaactgct aaatgctgat ctatatgaaa aaggacaacg
     2521 ggcacaattt tcttcttcct tgacaaaccc gtcttctcgt tttgtagatc accttgctta
     2581 tttatcgatt ggtttagttg gcggcctatt ggcattgaag agtggttcgt caatcactgt
     2641 tggtgtgatt tccagcttta cgatctactc aagtcagttt tctaaaccat ttatcgaact
     2701 ttctggaatc acgacacaac tgcaaaccgc tttttctggt ctagaacgag catttgattt
     2761 gatcgatcag ccagaagaaa agcctgattt gccagatgcc tatgccttgg aacccaaaaa
     2821 tatcaaagga aaagtctctt ttgatcatgt ttctttttct tatgacccca agcagcgttt
     2881 gatcgaagat ttcaatttta ctgcttatcc aggtcaaacc attgctatag taggaaaaac
     2941 aggggccgga aaatccacac ttgttaattt gctgatgcgt ttttatgaca caacaaaagg
     3001 aacaattaca gtcgatgacc atgacatccg ctctatccaa cgtgatacat tacgtaaaag
     3061 ttttggaatg gtgttacaag atacttggct atttgacagt acattgcgag aaaatcttcg
     3121 ttttgggaat ccggaagcaa gtgacgcaga aatcaatgat gccctaaaag cctcttatat
     3181 gtatgaattt gtagaacgtt tgccggaaaa attagatact aagattggcg gtcaagggtt
     3241 aaagatatca gatggacaac gccaactgtt gacgattgct cgaacgatga tcagtaatcc
     3301 ccctatgttg atcttagatg aagctaccag ctcagtcgat acattgacag agaaaaaaat
     3361 ccaacaagcc ttcttagcaa tgatggaagg aaaaaccagt tttgtgattg cccatcgcct
     3421 gtccacaatc aaaaatgcgg accagatatt agtgatggat cagggagcta ttgtcgaaat
     3481 gggcacccat gatgagctgc ttcaaaaaga tggttattac cgacggctct atcaagcaca
     3541 attcacagca ca
//
LOCUS       DAXWLF010000207         3490 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXWLF010000207 DAXWLF010000000
VERSION     DAXWLF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.211"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            44..805
                     /locus_tag="ACGE4U_002562"
     CDS             44..805
                     /locus_tag="ACGE4U_002562"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8394658.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(920..1360)
                     /gene="fabZ"
                     /locus_tag="ACGE4U_002563"
     CDS             complement(920..1360)
                     /gene="fabZ"
                     /locus_tag="ACGE4U_002563"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFF8394659.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            1580..2335
                     /gene="fabI"
                     /locus_tag="ACGE4U_002564"
     CDS             1580..2335
                     /gene="fabI"
                     /locus_tag="ACGE4U_002564"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFF8394660.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(2445..3458)
                     /locus_tag="ACGE4U_002565"
     CDS             complement(2445..3458)
                     /locus_tag="ACGE4U_002565"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFF8394661.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
BASE COUNT         1094 a          700 c          643 g         1053 t
ORIGIN      
        1 tatcattaaa gataacaaaa tagtaaattg gaaaggaaca gttatgaaga aaaataaagt
       61 tattttaagt attagtttag cagcagcatt gctgtttgct gctggttgtt ctgcaaacaa
      121 cacgacttct tctagttcgt ctacagaaag ttcaaaaagt acatctactg cggaaagcac
      181 gactgccagt agcgtcgatt tgaacgcatt ggaattacca caattgacga aagatgtcgc
      241 cgaaaatgaa gatctggtcg aaatggtgac gaataaaggc acaatcaaaa tcaaattatt
      301 tcccgaacac gcaccaaaag cggttgaaaa ctttatgaca catgcaaaag acggttacta
      361 caacggactg actttccatc gcgtcatcaa agatttcatg atccaaggtg gcgatcctaa
      421 aggcgatggc actggagggg aaagcatttg gggagaagga tttgaaacag agatcaacaa
      481 ccacctttat aatataagag gtgcgttatc tatggctcgc tcacaagatc ctaacagtaa
      541 cggcagccag ttctttatcg ttcaaaatac agatgatatg catgatggtc ttttaaaaga
      601 cgactatcct caagcaatca tcgacgctta taaaaacggc ggttatcctt cactagatgg
      661 ccattatact gtcttcggcc aagtgattga aggaatggat gtcgtggatg ctattgcaaa
      721 tatcgaaaca gatagttcag acaaaccaaa agaagaagta aaaatcgata aaatcaatat
      781 actgcaagaa gcaaaacaaa aataaaaaaa taacgtctga atcctgcata gatgacaacg
      841 tatgctagga ttcaggcgtc ttctttttac ataaaaagca agtaagcttc cttttgtttt
      901 gctatatcag gttttatagt caagcatttt ggttgacgat gaacgtgaac tcgcactcac
      961 agactttttt atcatctaca tatgctgcag ataacgctgt accgactgat cccttcattt
     1021 tggtgatttc aaaatgacat ttcagcacat ctcctggaac aactttttga cggaatttgg
     1081 ccttattgat cccaccgata taagctcttt ttcctagaaa ctcttctgat tttaggataa
     1141 gtatcgaacc tgcttgtgcc agtgtttcta gaatcaacgc acccggcatc actggattgt
     1201 caggaaagtg accttggaaa aaatcttcat tgattgttac attttttgtc gcaacgatct
     1261 tttttcctgg ttctaaagta tctacataat caatataaca aataggataa cgattaggaa
     1321 tcaattccat tacttcagta gcagataata cgtaagacat aattaaactc cttctttcag
     1381 gcaattgcca ttttcattac tttgactatc aaagtatcat attcctaaat atttagcaat
     1441 ctttttttaa tttaagaaaa aatagtttga caatcaaaac attttgacta ataattaaga
     1501 aatcaagaag ttattttagc tattattctc tattttttta cgactatcaa atttttatca
     1561 gaaacaaagg agagtcatta tgtcatttct agaaggaaaa aaaatcgtca ttatgggcgt
     1621 cgctaacaaa aaaagcattg cttggggttg tgctgaagca atgttgaaac aaggggcaga
     1681 aattatctat acttaccaaa atgaacgaat gaaaaaatca ttagtcaaat tggtcggcga
     1741 agaagcattc actgtagaat gtgatgtttc tagcgacgaa agcatcgaaa aagcaatgaa
     1801 tgccatcggt gaatatgctg gggagatcca cggccttgtc catgcagttg cttacgccaa
     1861 caaagaagaa ttgtccggaa atgtgtctga tatttccaga gacggctatc aattagctca
     1921 agatatcagc agctactctt taattgctgt atcaaaatac gcacaaccgt ttcttgcacc
     1981 aaacagcggg atcgttagca tgacttatct aggttcagaa cgcgcaatcc cgaattataa
     2041 catgatgggt attgcgaaag cttctcttga agctgctatc cgttatttgg cagctgagtt
     2101 ctcgcctaaa ggaattcggg tcaatggaat ctcagctggg gcaatcaaaa cattagctgt
     2161 cacaggtgtt aaagactatc aaaaactgat ccagttatct gaatctcgta cgcctgatca
     2221 gcaaggtgtg acgatcgaag aagtcggtaa tgcttgtgct ttcttaatca gcccactttc
     2281 cagcggaatc atcggtgata ttatctacgt tgacaaaggt gtccatcttt cataaacttt
     2341 tgttgcattc tttaaagtaa aacatataaa aatcaggaaa aaacttttta aagtctcttc
     2401 ctgatttttt ctttttacgt ttttttcaac tcattacttt gaaattattc cacgacttca
     2461 atccagcctt ctggtgcttc tatgtctcca aattgaatac ctgtcaattc atcatacaac
     2521 cgttttgtta ctggtcctac ttccgtttca ctataaaata catgaagatt gttattgtga
     2581 taaatcccac cgattggaga aataacagcc gctgtaccac atgcaccagc ttctgcaaat
     2641 tgatctagat catcaatgaa aatatctcct tcaattgctt gcagtcctag tcgttcttca
     2701 gctaactgca acaaggaata tttcgtgata cttggtaaga tagagggcga ttgcggagtg
     2761 atgaattttc cgtcttttgt aattccaaag aaatttgctg cacctacttc ttcgattttc
     2821 gtatgtgtgg ctggatcaag ataaacacaa tctgagtaat ccagttcttt tgcttctgtt
     2881 cctggaagta aactcgctgc gtaatttccg ccgacttttg ctgcaccagt ccccttagga
     2941 gcagcacgat cgtaatcaga tacaataaaa ttcgttggtt ttagtccacc tttgaaatac
     3001 gctcctactg gcgtacagaa gacgacaaat aaatattctg gagctggatg gacaccaatg
     3061 ttttcaccga ccccaatcag taaagggcgc aagtataatg tagcgcctgt tccatagggc
     3121 ggcacaaaat cttcatttgc ttttaccact tgcttgacag catcaacgaa catcttttca
     3181 gggaccattg gcatcagcaa tcgtctagca ctttgattca tccgtcgact attttgttca
     3241 ggtcgaaaga gattgacctt gccgtctttt cgacgataag cttttagacc ttcaaaacat
     3301 tgctgaccat aatgaagcgc tggagagcct tcatgaatcg tcaggtaatt atcttctgtc
     3361 aattctcctt cttcccattg tccgtctttc catttggcaa taaaacgcca aggagttttg
     3421 atataagaaa aacctagatt gttccaatcg atttgcatat acttaccacc ttcgttattt
     3481 tctccacatt
//
LOCUS       DAXWLF010000208         3488 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXWLF010000208 DAXWLF010000000
VERSION     DAXWLF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.212"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..2232)
                     /locus_tag="ACGE4U_002566"
     CDS             complement(<1..2232)
                     /locus_tag="ACGE4U_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8394662.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACGE4U_002567"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACGE4U_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          982 a          771 c          549 g         1186 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaata
//
LOCUS       DAXWLF010000209         3469 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXWLF010000209 DAXWLF010000000
VERSION     DAXWLF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.213"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            116..1003
                     /gene="xerD"
                     /locus_tag="ACGE4U_002568"
     CDS             116..1003
                     /gene="xerD"
                     /locus_tag="ACGE4U_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFF8394663.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            1022..1393
                     /locus_tag="ACGE4U_002569"
     CDS             1022..1393
                     /locus_tag="ACGE4U_002569"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8394664.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            1390..2160
                     /locus_tag="ACGE4U_002570"
     CDS             1390..2160
                     /locus_tag="ACGE4U_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFF8394665.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            2144..2713
                     /gene="scpB"
                     /locus_tag="ACGE4U_002571"
     CDS             2144..2713
                     /gene="scpB"
                     /locus_tag="ACGE4U_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFF8394666.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            2719..3435
                     /locus_tag="ACGE4U_002572"
     CDS             2719..3435
                     /locus_tag="ACGE4U_002572"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8394667.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
BASE COUNT         1196 a          608 c          778 g          887 t
ORIGIN      
        1 aaagaaacca gtccgtctga gtgtctagac ggctttttct ttgctcagat tatggaaaaa
       61 gactatcaga atgaagcggt atctgatatg atagagaggc atgaggggga ttattatgaa
      121 agagcaaatc aacgattatc tccactactt gaatattgaa cggggattgt ccgccaatac
      181 acgacaaagc tatgaacgtg atttggaaca gtacttgcat tttctacagg agcagaaaat
      241 tgcttcatgg caggatgtcg atcgcttttt agtcatccgt tttctggaga agttgcatga
      301 agaaaaaaaa gcgacagcaa ctgttactcg gatgatctca agtctcagaa gattccatca
      361 gtttttacgg caagagcgta tcactgatca tgatccgatg caacatatcg atacgccgaa
      421 gaaagcacag aaattaccaa gtacattgac tctgacagaa gtagaaaagc tgattgaagc
      481 acctgatacg acaaaaacgt tagggataag agatcgtgca atcttggaag tcatgtatgc
      541 tactgggatg cgcgtcagtg aattgattgg attgaaattg ggagatcttc atctttctct
      601 tggactggta cagacagttg gaaaaggaga taaagagcgg atcattcctt taggtgatta
      661 tgcgatccaa tggctggaac gctacttaga cgaagcacgt ccattacttg taaaggacgc
      721 atctgaaatg catgtatttg tcaataatca tgggaaaggt ctttctcggc aaggaatctg
      781 gaaaaatctg aagcagttag tacgagaggc tgggatttac aaagaagtaa caccacatac
      841 gctgcggcat agctttgcca cacatctgct agaaaatgga gctgatttgc ggacagtaca
      901 ggaattgtta ggtcacgcgg atatctctac gactcagatt tatacacata tcacgaagaa
      961 aagaatgacg gatgtttata agcagcattt tccaagggct tagtcatagg aaggtgagaa
     1021 gatgttaaca cattaccgga aagaaaaccg taaaattgct atgggactat tgagtttcca
     1081 taagaaactg acaggtaaaa cttctttgtt gaaggagatt gatacgtacg aatctgaaga
     1141 cgattatgaa ctttttttct ttacgccaga aggttcaacc aacattcaag ggattattgg
     1201 tatttactgg gcaacgaacg atcagctgat catccatgac atttctttga atccgtctta
     1261 tcgcggtgag aatatgggct ttcaaatgtt agatgaatta caggaaaagt acccggatgt
     1321 ctccatccgt gcaactgaaa tcacagagcc ttacattgga aaatggctag caaagaaaaa
     1381 gaagaaagag tgaatgagtt gacggagatc aatattaaat tagatgtatt cgagggtcct
     1441 ctcgatctgc tgcttcatct gattcagcaa atggaaatcg atatatatga tataccgatc
     1501 gctacgatca cggagcaata tatgaattat atccatacaa tgaaaacact cgagctagct
     1561 gttgcaggag aatacttagt gatggctgct acattgatgg caatcaaaag caagacattg
     1621 cttcctgtcc aagaattcgg cacagacgat gaagaattat gggaagaaga tcctcgagaa
     1681 gaattggtca ctcagctttt agaataccgt aaatataaat atgcagctga aagactgaca
     1741 gaaaaagcag aggaacgaag tctttattac acaaaagaac ctatgaacat cgatgggtta
     1801 gcggatgctg aaaagccgtt gaagcgagga caagtcaaaa aaatcgattt gatccgtgcc
     1861 atgcagaaaa tcttagaacg taagaaattt tctcaaaaag tggaaaaaac gatcacgccc
     1921 gatgacacaa cgatcgaaga acgaatggta tttatcgaag agcgtataaa aagaaatgcg
     1981 catggctgta ctttcgattc tttttttgag tcaccttcta aaagtgaagt tgtcactact
     2041 tttatggctt tgctagaatt gatgaaaaaa gggcagatca ttgcgcaaca aaaagaaaac
     2101 tatgaatcaa ttatgcttta tccagcagta ggagataccg aacatgacga aattaagtga
     2161 acttgaagcc cttcttttta ttgttggcga ggaagggatc agtaatgaag aattaagcgg
     2221 actgttaaat ctcagcgaag cagaagtcac aagtttagtg aaagaattaa aagaatctta
     2281 cactcaaaac tcgcactcag ctttgcatat ttttgaaacg gaaagccatt atgtattaac
     2341 gactaaaaaa gagctggctc ccttacttaa gcgatatgcg caagggacat caaacacatt
     2401 atctcaagcc gccgtcgaaa ccttagcaat catcgcttat aaacagccaa tcacaagagc
     2461 tgaaatcgag cagattcgag gagttcaggt ttcaggagcg gtacagcgtc tagctgccca
     2521 taaactgatt gaagaaaaag gtagagtaga aggaccaggt cggccgatat tatatggaac
     2581 aactggctac ttcttagatt attttggttt gaaatcatta gaagaactgc ctgatacaca
     2641 agaaatggaa atttcacttg aagaagacgt gtcaccagat ctattctttg atggaatgaa
     2701 taaaggagaa taattgtaat ggagagattg cagaaagtaa ttgcccacgc tggcatcact
     2761 tctcgtcgaa aagcagaaga attcatcctt gcaggacgtg taaaagtaaa cggacaagtt
     2821 gtaagggaat tagggataaa agttgggaaa catgacactg tggaagtgga tgaagtccca
     2881 atttatcaag aagaatacgg ttactattta ttgtacaaac ctaaaggagt tatttctgct
     2941 gttgcagatg acaaaggaag aaaagttgtt acggatcttt tgccgatggt aaaagaacgt
     3001 atctatcctg taggtcgact ggattacgat acttcgggga tcttgctact gacaaatgat
     3061 ggagattttg ctcagcgcct gacacatcca aaacatgaag tggataaagt ttacgttgca
     3121 aaggtaaaag ggatcgctac aaaatcgatg cttgcacctt tgacaaaagg aataaagatc
     3181 gaaggacgaa aaacatctcc agctaaattc caaattttat cagtggatcc taagacaaac
     3241 cacagtatcg tggaattgac gatccacgaa ggacgtaatc atcaagtgaa aaatatgctt
     3301 caagcagttg gtcttcctgt gcaaaaacta aaacgtgaaa aatacggaga tctgacttta
     3361 caaggcctac gtcctgggga ttaccgtgaa ctgaacaaaa aagaagtcag cagtttgctg
     3421 aaccactcaa aatgaacact tactttttat aattgttaga tgttgcgtt
//
LOCUS       DAXWLF010000210         3459 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXWLF010000210 DAXWLF010000000
VERSION     DAXWLF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.214"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(149..1102)
                     /gene="menA"
                     /locus_tag="ACGE4U_002573"
     CDS             complement(149..1102)
                     /gene="menA"
                     /locus_tag="ACGE4U_002573"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFF8394668.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1115..2218)
                     /locus_tag="ACGE4U_002574"
     CDS             complement(1115..2218)
                     /locus_tag="ACGE4U_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFF8394669.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2308..3243)
                     /gene="pplA"
                     /locus_tag="ACGE4U_002575"
     CDS             complement(2308..3243)
                     /gene="pplA"
                     /locus_tag="ACGE4U_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFF8394670.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
BASE COUNT          972 a          715 c          610 g         1162 t
ORIGIN      
        1 actttttcac aacctcctga taaacggtgt tcaaaagctt gcatctgcgg taataagccc
       61 aaccaaacga aaaatataag gagctatttt tatttggctg ttcttattac ttgatgcagg
      121 gcagtttttt cacaacctct tcatgctgtt aaatggcttg cataatgatt gttagtaata
      181 gccccaatac ataactggca ttgaataaga ccatattttt gatggaatag ctgaaactga
      241 taggctgggg taacgtttgc ttgaattgat ccaaatgctt cttcacaatt ggcaaggtca
      301 agaaggtgac caagatcggc cattcataga taccgctcaa caaccccatt aaaatcacga
      361 aatagcaagc atacatcaat agctggaata agactgttcc cgcaggacgt ccgatataaa
      421 aaactagtgt atagcgatga ttttcaatat cacggtcaag atcccgcaaa ttattagcca
      481 acatgatatt ggcaatcgta aacaccaagg gtaaggaagc taatacgatt gctgctatag
      541 cccaaagatt tcctaccaaa tcaaattgtc cagatgaaaa gttgactgtc agatcataga
      601 catctgaacc gaatgtattg acataaacga ccatggcaaa aattccaagg cccatagtca
      661 ccccactgaa aatttcccct aaaggcattc gagataaagg aatcggtccg aatgtataga
      721 atactccaat aaaacaacag ataaatccca tcaaaagtaa aagccaaccc gtattgatcg
      781 ttagaataac gccgataaca aaaacaaatg caatcatagc taaaatcatg tttcgaatca
      841 atacaggaga tagatttctg cggccaatga cattttcttc atacttatat acttcagaat
      901 gagccttttc ataatccatg taattattga tagcggtagt cgctaaatca aaaactagca
      961 ttccaataaa aaataaaaaa gtgtttagcc aatgtatctc atgaaaaaaa gcgatagaga
     1021 ataatacccc taccgcaaaa ggaaatacac tagccacctt tgttcgtatt tcaactactt
     1081 ctaaaaagat ttttagtgtc atggttcgta ctccttattt tagttcagaa cggtcaccca
     1141 tcgtgtaatg ggagtcctcc gataatttga aggtgtcttt gattggatcg gtcacatata
     1201 ctttatcgtc ttttgtcacg aagatcgctt ccgtcccgtt attcaattcg ctttcgacgt
     1261 attccagtcc ttttttcaca cccattgaaa agactgctgt agacaacccg tcaccatcaa
     1321 ttgatttatc tgttatgatc gtgacacttg cgatatcatt gtcgaacgga taacccgttt
     1381 ttggatcaaa caaatgatgg tattttttgc catctacttc taaataacgc tcgtaaattc
     1441 cagaggtaac taatgttttg ttccgttctt taatagaacc taaaacagat ccgcgagcca
     1501 tgttcggatc ttggatgccg actgtccagt cttggttttc gcctcttggg ctgtggccta
     1561 agacatagac attaccgccc aagtcaacga tggcagtggt cactccttgt tttttcaaga
     1621 ctttcactac ttcgtccgta atgtaacctt tggcgattgc acctaaatcg atgatcattc
     1681 ctttttcttt tagataaaca gttttttctt tgtcatttag ttcgatctta tgataatcca
     1741 ctaatttcaa tgcctgatcg atttcttctt gggaaggttt tctggcgtca tcaaaaccga
     1801 ttcgccataa ttgtgtgatt gcaccaatcg ccatatcgaa tccgccttga gaatcttggc
     1861 tatattcgta ggcccgcttg actaacgtgt agacgtcatc agaaactttt actggtttga
     1921 tcccagcttg ctcattcact tcatcgatct cagaaccttt ttgattgatc gtgattttat
     1981 caccaagttc ttttactcga tcgaaggcag gtttcaatgc tgattctttg ccttcatcat
     2041 acacacggat acgaacgtaa gtccctaaca agaactgttc atcagaatat ggatctttca
     2101 atatcttggt agctgctgaa ctttcagtgg ttgttttatt tgctgagttg ctgcagccag
     2161 ttagcaccat aaaagagatg cccagtgtga ttaaggaaaa tagtagtttt ttccgcatct
     2221 ttttcctctc ttcattttga aaggtgagac gactgcctaa tctggctatc gtcccaccct
     2281 ccaactttta ttagtatgat tatgtgatta tttttctgtt acgatattat ctacttcgat
     2341 tgtttgagtg tcgccttttt cagctgcatt cactaattgt tgtgcgtaca tgatgaatga
     2401 atgtgagctg tgtgtagcac ctgtaacaac ttctacatta gcaggtgagc catcttcttc
     2461 gcccattgct ttgacgaatt cgtcgttcag agtagggatg taagtttttg gttctgtacc
     2521 gctttttgct ttcatatttt cattatattc tgtatcatct tgttttgatt taccgtcttt
     2581 gtcgatataa tcaaagtttg attcagttac tttgccgcca gcaactgtca tttcgaattg
     2641 cacacggtag ccgttagagt agttcttttc ttcaagcttg taagtgccgt ctttcaaatc
     2701 agcaccattg tcgatttcga ttgtatctgt gttaccagct tgagcagctt ggatcaattg
     2761 ttgtgcgtag ttttggaagc tttcagatga gtgagtagct ccagttacga cttccacacc
     2821 gcttgcgctt tgtgctttta agaattggtc attcaactca ggaatgtatt tttctgggtt
     2881 tgtgccagat tttgcttcca tgtttttgtt gtattctgca tcttttgttt ttgattcgcc
     2941 gttttcattg acgttatcat attttgattc agtgattttg ccgtctttta caaccatttc
     3001 aaagactgca cggtaaccat ttgaataatt tttttcttct aatttatatg tgccgtcttg
     3061 caaatctccg ccagctacaa cttttgcaga tgattctgtt gtgctttttg cagtcgaaga
     3121 ttgtgctgta gatgattcag aagaagagtt tgagcttccg ttgtcatctg cgccgcaagc
     3181 gcctaacact agagctgaaa atgctaatac ggcaaatcca gttacaaaac gttttttctt
     3241 cattttaata ccccaccatt ccatttctag tccacaattt ttctttgtga ttcattatac
     3301 attctaatct ttttagccta taagtcaact atttttgata aataacccat gtaatatttc
     3361 aaacgttttc tttcagcaaa tttcgtgaaa aaacgcttta ttttaaaatt tgaaaaaaat
     3421 ccaaaaggag ttagacaaaa gaacagaaca tctatataa
//
LOCUS       DAXWLF010000211         3451 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXWLF010000211 DAXWLF010000000
VERSION     DAXWLF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.215"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            5..694
                     /locus_tag="ACGE4U_002576"
     CDS             5..694
                     /locus_tag="ACGE4U_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFF8394671.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            776..1825
                     /locus_tag="ACGE4U_002577"
     CDS             776..1825
                     /locus_tag="ACGE4U_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8394672.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            1941..2678
                     /locus_tag="ACGE4U_002578"
     CDS             1941..2678
                     /locus_tag="ACGE4U_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFF8394673.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAIYQKG
                     KRIMVPLPVQKNVLSIKSEDVFLDFFF"
     gene            complement(2661..3158)
                     /locus_tag="ACGE4U_002579"
     CDS             complement(2661..3158)
                     /locus_tag="ACGE4U_002579"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8394674.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(3214..>3451)
                     /locus_tag="ACGE4U_002580"
     CDS             complement(3214..>3451)
                     /locus_tag="ACGE4U_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8394675.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT          986 a          613 c          684 g         1168 t
ORIGIN      
        1 aaatatgaaa aagaaaataa tccgattgat gttgatctcg ttgggaatca ttctgatact
       61 ggtagtgggc tatcggttgt atttagaata tcagacagac atccaattat ttttaaatcc
      121 gagagcgagt aaggcagagc tactcgcaca tattcgttcg catgggactt ttgcatcgat
      181 actcttgatc gtattgacag cggtgatgtg tgctgttcca ggtattccta cttcagtagt
      241 tggagtcatt gctggtttga gctttggacc actgacaggt gcgatcatca acattttggg
      301 taatgctctt ggtaacatca ctgcaatttt ccttatgcat cgcttaaaat ttttagataa
      361 gaaaaccgaa acgaatcatt gggtgcaagc aatccgacaa atgaaacatc caaaaatagg
      421 tgtaatgtta ggctatatga taccggttat tccatcttct atggtcaatt ttgctgcaga
      481 tgcgatgaaa ttgcctctac agcaaatcat attgtcgatc gtgattggag tcatcccttc
      541 gtctgtgttg tatgcgtgtg gtggagaagc tctgtttcac ggatataaca aaactgcagt
      601 cacgttggtt gcaagtgtta ctgtgctagt agggttagtt gtcttcattt ataaagatcg
      661 caagaaaaaa caaggaaaac aaaagcacaa ataataagtg tggcgaatag ttgtattttt
      721 ctatttccaa tcttttccat aagttgtaaa ctaagatagg aaatggggga aatcaatgaa
      781 gagtaatgaa aaaacacctt taacaacttc gcttatttta ctattatcaa tcacttgtgg
      841 cgttgttgtt gccaatatgt attacatcca gccgattgga acaaaagtag cagctagttt
      901 atctgttagc acaagtgcag tcgggatatt gacgatgttg acccaattag gctatgcgtt
      961 agggttactg tttttagttc ctttagggga cgtggtggat cgtccaaaat tgatcattcg
     1021 aatggtagcg ttatctgccg tctctctgtt ggccgctttt tttgcgcctt cgtttacgtt
     1081 atttgcatgc gcttcttttt taataggatt actttcgatc gtgccgcaaa tcatcattcc
     1141 ttacggtgcg gtactagcgg gaccaaaagc aagagggaag gtcatgggta cattgcttag
     1201 cggtttactc gtggggattt tgctttctcg gacggtatct ggtattgttg ccagtctttt
     1261 ttcttggcgg atgatctatt tatttgcttt atgtgcagtc ggaattctta cacttctgct
     1321 ttactggaaa atgccgcgtt ctgtccagca aagtagtaca gctgtttcat accttgattc
     1381 acttaaaagt ttgccgcatt tagttgcttc tcaacgtcta ttaagagaag cggcagtcag
     1441 tggatttttc atgtttggaa cattttcgat cttttggtct acgttgattt tttatatcag
     1501 cagtcccgcc tacaattggg gaacattcga agcagggata ttagcgattt ttggtttgtc
     1561 tggagcagtt gctgcaccta tcgtgggacg actggcagat tcttattctg aaagaaaaat
     1621 cgtgtatatg ggacttgtta tgcaaacaat aagctttatt gctttattgg ttggcggaag
     1681 ccatttggcc attttgcttt tagctattat tttattagac gtcgggaatc aatttggtca
     1741 agtcgccaat caaacacgag tacaagggct aggtgaagca gcaagcaacc gaaataatac
     1801 tgtatttacc cgtggtgcta gttaattttt tcctagagga aaatgtctaa acatgttggt
     1861 aaatcaacaa aaaaacgcat atagtagtct caaaactccc aagaaatgag gactatttaa
     1921 caaactacga tggttacgtc gtgacaactg aacattttga cttagccaat cctgttacta
     1981 aagtcgatga tatccccgat tatgaaatgt acagtcaaac aatcgatagc ttaaataagc
     2041 gttttggcaa tcgtgtttta aaaggcatcg aaatcggtta catcgcttct gaaaaggatc
     2101 ggatcatcga ctatttagcc gataaagatt acgatttaaa attactcagt gtgcatcata
     2161 atggtcaatt tgattattta gatgacgaag tcaaagatat ggatcctgct attgtgattc
     2221 ctcagtattt tgcacaactt tcagaagcac tagttgttat agaagcagat gtctttgctc
     2281 attttgacta tggttttcgg gtatttggtc tatccgttgc tgaatttaaa caatatgaag
     2341 cgcagttttt accgattcta gatcaagtaa ttaaaaataa attggcattc gaattaaatg
     2401 ctaagagcgc ttacttgtat gacaatttag cgttatatga atatgtcatc gatttatatc
     2461 tttcacgtgg tggtaccttg tttagtgttg gatcagatgg gcattacctt gaacatttcc
     2521 gtttccattt cgatgatctt tttgctctgt taaaagcaaa aggtgtgacg gaattagcga
     2581 tttatcaaaa aggaaagcga attatggttc cattaccagt ccaaaaaaac gtcctcagca
     2641 tcaaaagtga ggacgttttt ttagatttct ttttctaaaa tcggacaaat acgatcttgt
     2701 tttcctaaag gaaaatgttt gatcccgatt tttttaaatc catgacgttc ataaaaacga
     2761 atacctgcat gatttttttc ataaacagtc aaacgtaaat aacgtttttg tttcgtttta
     2821 gcataacgat tcgcttcatc gagtaaccgt tgccctaacc ctttgttttg ataatctttg
     2881 agcagataga gccgttgaat ctctatagca tctttttcat cattgacttt catataacca
     2941 atgagtattt gatgatcctt aataccaata aatttactgg ttgactgtgc caattctact
     3001 gccaatattt cagttgtcaa ttgtgtctgt aaatatcctt gcatatcaag tggatctgcg
     3061 ccatcgataa acgattcata aaaactgaca acacttacaa cttgtaaagc atctagatca
     3121 tcataatcta aatacgctag tgtgatttga ttgttcatct aactcctcct tttgacatag
     3181 tatactataa atcacccgtg gtgctagtta attttaatat cctaaagaat atctcaaagt
     3241 tccttcaaaa cgtcgttgtg ctcgacttaa tagaacactg taagccagta atatgctttc
     3301 aaatctactc tctagccctt taacagaacg tgagttgaaa ttttgacaac ctaatttttc
     3361 taaggaagaa aaaacagtct caactgtttt tctttgtttt ccaagtaagc tgtggtctag
     3421 tttttctgat ttatccatgt ttttacgtgg c
//
LOCUS       DAXWLF010000212         3427 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXWLF010000212 DAXWLF010000000
VERSION     DAXWLF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.216"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(77..149)
                     /locus_tag="ACGE4U_002581"
     tRNA            complement(77..149)
                     /locus_tag="ACGE4U_002581"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Gln,seq:ctg)
     gene            complement(195..623)
                     /locus_tag="ACGE4U_002582"
     CDS             complement(195..623)
                     /locus_tag="ACGE4U_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394676.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(683..1441)
                     /locus_tag="ACGE4U_002583"
     CDS             complement(683..1441)
                     /locus_tag="ACGE4U_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8394677.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            1736..1933
                     /locus_tag="ACGE4U_002584"
     CDS             1736..1933
                     /locus_tag="ACGE4U_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394678.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2039..2521
                     /locus_tag="ACGE4U_002585"
     CDS             2039..2521
                     /locus_tag="ACGE4U_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394679.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(2579..3175)
                     /locus_tag="ACGE4U_002586"
     CDS             complement(2579..3175)
                     /locus_tag="ACGE4U_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8394680.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         1036 a          566 c          655 g         1170 t
ORIGIN      
        1 aaaaataata caataatccc cctgcaaaaa gattgttctt ttacaggggg ttcatttttt
       61 cagcaagcac atcaaattgg gatggcaggg ttcgaaccta caattgcttg attcagagtc
      121 aagtgcctta ccagtttggc tacatcccat ttattcaaca aaataatcat agcactttcc
      181 taaaaaaacg caagttattt ttcttgatcg gatagttgtt gctttactcg taaaaggcgg
      241 cgtactagtt tcttttctaa tccagcagga agggcaatca gttctgctac tgacaagacg
      301 gtgtccaata actgatattc tttgacaggt tcattcataa tatgatagat gtttgctaaa
      361 cgttcaatat attcaaagcg agtatactct gttcgagcaa ttagaaattt caataaatcg
      421 gtggctttct cgtactcttg atctgcgtaa taaacaagag attgacgata caatttgatt
      481 tccagttcat tgagggtagc aggtctgcga cgctcgttcc actcattaat agagacttta
      541 ccttgaacat ttcccttgca gtcacggaca aaaataatcg gaatttcatt tggatccatt
      601 tttttatgtg tgaatttttc cattatctac gctcccttca aaggtttggt atcttgagta
      661 taagggattt ttgtttgaat tactagcttt tctccaaagt tagcatctgt ccacccattg
      721 cagtgaaaat cgccgaaaaa tcgttgatcg gtactgctcg attttttaca ccatagggat
      781 cgttgacata aatattttct ttgtcatatc cagtgactac acaagcatga acaagaggag
      841 tgactcttac ttctccatta tttgtggtcc aagtcataaa atcagtttca tctgggactt
      901 caaaattgat agtcacagaa gtccagacag gatttccttt agatagtgct tttttgatat
      961 catcgagttg agcatcggtt tttggaaaag tgtgataatc cgtttggacg atttgctgag
     1021 caagggaggc aactggggga acatagacga ccatagccca gtctccgcca ctgatatcac
     1081 caacaaatcc ttcattagga tcgccatgag tcccatctgc attgaaaaca gggacgtaat
     1141 ccaattgctc agctaactgg tttttagttg tttcataacc ataaaaagat aaaagcatag
     1201 aaagcgaagt gacttcacag ccattttcta aagaaggaga atcaaactgg ctctctaatg
     1261 gaacatctag taaaacttct gctttctctt ttgtcttttt cgttttttgt gttgaacttt
     1321 tttgtgcagt gttcattgta tgttctggag aatggatatg taggtaataa cttgtaccgc
     1381 ttaataaaag agcagctgtg ccccctagaa acaatagttt tttgaacgtt tttttcttca
     1441 tttgaaatct cccttttcag ctgtttatct ttcctattat aaatgtattt ttattggaaa
     1501 tggaagtatt atattttaat atgtaggaaa taattagttt ttaaggaata tgttttaatt
     1561 ttttgaaaaa aattcttcta aatacaacag ctgttttaat tgtgaaaacg aaaacagtac
     1621 gctataatgc ctgtaaggtt gatttttaac tgtaaataaa aatcaatgag ttaaatctcc
     1681 aaagaaatag cagtggtaat gtggagaaaa gttccagcag tttaggagga ttttgatgac
     1741 ggaagaaatg attaatttag gagaacaata tgcatgcaaa ccaattggtt ttacaaaaac
     1801 ggtcattggt gaagttgtat caaaaatgac aaactgcgcc gtggtcaaag ttgcccaatg
     1861 tgctgcagag gaccaagaat tattagatga aaaagcaagc atggttgttg caaaatatga
     1921 tacatttgaa taaagaaaac aattggacgt gcggcgtgct gcaagtccaa tttctttgag
     1981 aaggtaaatc agtattcttt tcctattttc tggagaagaa aaagcgaggc gagttgtcat
     2041 ggtaacattc tttacggttc tattgttttt attcgttgtt gtgatgtttt tatttctttg
     2101 gctggttcga aaagaaatta tttatcggac tgtgcgaaat cgatgggtct acttggtgat
     2161 acccttctta gtgtcactag tcatttggta tacactcatc agtcatccta cagcagatga
     2221 attggcaaaa gggatcttgt cagcaatgat ttttattagt tttttgttgg acagtagagg
     2281 aatcacagaa gaaggacttg ttttgaatag ctttgacaaa aaaggtgttc ctttttctga
     2341 gatcaataaa attgtattat atcagccaaa aggttctaaa atcgtaaaaa tgaatttttt
     2401 ccgaaacgga tggcgaggac cgatgctgaa attttctgca tcattagaag aattagttcc
     2461 atttttgtcc caacgcttaa acgatgaggc agagattgat attatgattg atccagaata
     2521 aaaacagagg ttgcgacaag atgtttgtca caacctctgt tttttatttc aaataaaatt
     2581 atccaaatac gcggaagcga tcggcatcgt tcatgtattc tttttcccca gccggtgttt
     2641 ctggtgaacc gaatactaat tgcgaacgta gtttccagtt tgaagggata ttccattctt
     2701 ttgcaactgc ttcgtcaata actgggttgt aatgttgcaa gtttgcgccc aatcctttat
     2761 caactaatgc tacccaagta ttagctgttg cgattccgtt tgattgttct gaccaatctg
     2821 ggaagttgtc tgcatataat tcaaattgtt cttgtagacc ttttaccacg tcagtatctt
     2881 tgaagaataa tactgtacca taaccatttt tgaaaccagc taatttgttt tgtgtatttg
     2941 ggaaagcttc tgctggagtc aatgggcgaa gtgcttcttc agtgatttcc caaagttttt
     3001 catgtgcatc tccgaaaagg ataactgcgc gtgttgattg agcattgaaa gcagttgggc
     3061 tttctttaat tgcttctttg atcaaagttg ttaattcttc attagaaagt gaaacattac
     3121 gtcctagatg atagattgaa cggcggtttt tcaaagtgtc agtaaaagtt gtcataataa
     3181 aatttctcct ttgatttgcg ttttgcattt ttttaattga ttacgaaatg aagtatagca
     3241 ccttacaaaa agtaagtaaa cagatatgct cacaaaaaat aagcgaagtt caaaaaacaa
     3301 ttttaataag aaaaatcgtt atgtaaggat tttcttagtt atcgctttca ttgttgtgtt
     3361 ttaggaattt ttgcttattt gaaaaaaaca gctgatatat aattattaaa tctctatctt
     3421 ctttttt
//
LOCUS       DAXWLF010000213         3299 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXWLF010000213 DAXWLF010000000
VERSION     DAXWLF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.217"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(554..2167)
                     /locus_tag="ACGE4U_002587"
     CDS             complement(554..2167)
                     /locus_tag="ACGE4U_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8394681.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="ACGE4U_002588"
     CDS             complement(2308..3216)
                     /locus_tag="ACGE4U_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8394682.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1072 a          564 c          578 g         1085 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     3241 agctttctat ttataattaa tgactattat aacaaaaatg aaaagccaaa acgacaagt
//
LOCUS       DAXWLF010000214         3281 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXWLF010000214 DAXWLF010000000
VERSION     DAXWLF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.218"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            677..763
                     /locus_tag="ACGE4U_002589"
     CDS             677..763
                     /locus_tag="ACGE4U_002589"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFF8394683.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1065..1289)
                     /locus_tag="ACGE4U_002590"
     CDS             complement(1065..1289)
                     /locus_tag="ACGE4U_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394684.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1655..1939
                     /locus_tag="ACGE4U_002591"
     CDS             1655..1939
                     /locus_tag="ACGE4U_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394685.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1942..2358
                     /locus_tag="ACGE4U_002592"
     CDS             1942..2358
                     /locus_tag="ACGE4U_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394686.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2495..3202)
                     /locus_tag="ACGE4U_002593"
                     /pseudo
     CDS             complement(2495..3202)
                     /locus_tag="ACGE4U_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
BASE COUNT         1098 a          605 c          518 g         1060 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tacccttctg tatccgttgg gagagtaagg ctcaactgcc ccctaaaaag
      241 ggttctaaaa aatctttaaa aaataaataa aataaacgcg cgcgcgcgag caaaatttcc
      301 cgccaaaaaa tcacccactt tttaaaataa acaggttcgc gcatgatatt aaactgccaa
      361 tctagctccc tatggtcgct gtaagaagga gtgatcgcta cgctctcaca ttttacttgc
      421 agcagtcgcc tttaggctcc ttcgccaaaa ataattcatt agggactcct ttgtggcgtc
      481 ctgagagctc gtctaagccg ttctaagaca agattcgata aaaactatgt ccttcgttct
      541 aaagcgcctt acaggcggtc agggcaagcc ctaacaaccc caaggcaata aaaaacttag
      601 gcttattttt gcttttttat tgtataattg attgtcgcct atccgacagt cttgcgactg
      661 gaaggaggtg tgtttcgtgt tcactaactt tatttgctcc cttcttgttg ggcttttaag
      721 cgctttgttc agttactggt tgaataaccg aaacaagaag taaaacttaa ttaaggcgac
      781 tcagcccaca aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtgtttcgtg
      841 ttcacttatt tgcaatcaaa ttataacaga aataaatatt tatttcaaaa atatcctaca
      901 aaagtctaaa atccgtaatt atttcttata tgcaacattt acaaataaag tcgctctgag
      961 agctcgtatt tttcgattta agacgtttta tttagttttt ggtgtatttc attggtccaa
     1021 gctaaaaaac tcttagaagc gattttagag ccatttaaac ccacttaatt atattctcca
     1081 tgaaattcgc tccatgcgtc aaaagctgcg tctgaaattc cgccgtcttc gttcactagt
     1141 tgcattaatt tttccaacga tcgttttctt tcgggtgtca tatttttata ttccaacaat
     1201 ttttcgtctg ttaaatcgaa cttgattgct tgaattgctt gctctaattt ttctaagttt
     1261 tgaaaatatt cgtaaggaaa tttttccatt ttttctttct ccagtctcac aatttttcta
     1321 gaaataaaat cacttctttg ccgtctatac tctcaaaaaa aatgtcgttt gaaaaactaa
     1381 ataaattttt gatcgtgcat ttttaaattt ggttatcgat tacacgtgca gcactccccc
     1441 ccgaatttta aagagcataa ggaaacgtgt acccactttc taaaaaaagt gggtagatag
     1501 gctgtgccta tcacgtttct cagaaaaaaa tttgatatca ctttttcgcc atcgtgggta
     1561 gcttatgtat agcattttgg caaaaaacag ctatacaaat atagtatttg tatggtatac
     1621 ataaattata cataattgca aaaggagatt ttttatggac attttagttc ggaatattga
     1681 tccaaagttt gttgcaacaa tagacaaacg ttgccaagaa ctcacgaata aaacaggaga
     1741 aaaatggagt cgaaataaat atctaaaagt tttaatcgaa agtgattttg accgtcaact
     1801 tacgacttac aaaaaagacc aattcgattt attgctcgat aaatttattg ctatccaaac
     1861 agaaaatact caaatattaa gcgagtatgt ctacacaaat aatcaaatca ttgaaatgct
     1921 tatcggagga atgatataac catgagaaaa acttctgtcc aaaaacatgt tactttgtac
     1981 cctagtcatg ttgacgtttt agattccatc atcgaaaaaa atccagatat caataactac
     2041 tcggaagcca ttcgcttttt atgtatgagt tatgaacccg ataccaacac gaccactacc
     2101 agcaatcagg caaaactaaa tgctatggga aaagaaattt ccatcatgac tgaaatcatc
     2161 gcagaatttg ctgatttata tctaaaagat acaggcgttc ttattggcac aaattctaaa
     2221 gtatatcaag aagtgaaaga aaaaatcgaa gctaaactaa atccatcaaa tcgaaatcga
     2281 ccaaagaatc atcaaaaaga aaccagtcaa acagtcactt ctagaaattt cgacccaaca
     2341 aattttctaa aacgataaaa aacagatagg agttattcct atctgttttt tatttagaag
     2401 ccccatacaa tatgctagaa tgtactagca atcttagtcg gttgtaaggg acttgtctac
     2461 ttgcttaatg taaggggggc gagttctttt ttatttattc ttctaaccag ttacgcagag
     2521 aaatctttgg caaatctgac gttaggcttg aaaaagtaaa ttctaatttg gcgattttat
     2581 tgcccttttt tgcttttatt tttgtgactt taagatcatt gaagtatttt tttagttctc
     2641 gtaatgcagg atttaatact ttttgatcaa tgttacccat ttgatagtat tctgggatat
     2701 ccattaagct cctaaactct tcaatacgaa ctacataata accagtagaa cggtactgca
     2761 taagcagtcg gaatagagtt tttgcatacg tagaacgcaa cgacgtgaaa gcagataatt
     2821 caaatctact aaattcagct gttaaaccat taataatata ttctaggtcc ggattaacac
     2881 tgacttcaac aaactttttt gttttattga tttcaaaacc agtaaacaaa acaaagtagc
     2941 gaatagaatc ctcatcttca gtacggtaag taagattaag catatcttta tagagctttt
     3001 ctaaatcacc gacaaaggct tttgtagcag tcatcttata atcacttaac tcttttaatt
     3061 cctcgaaaga aaatctaact tttttcaaac cacgatcttt catacgagcg cataatgcaa
     3121 aaaataaatc catctgctta gcattgaaat ttttcagcgg tactaaattc aattcatttt
     3181 gatatttgac tgttaattcc gttggttctg ttgcaaagtt ttaaatctac tatcaaataa
     3241 ggtagaataa tagaaaaaga tagcaggagg aatgacgatg a
//
LOCUS       DAXWLF010000215         3257 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXWLF010000215 DAXWLF010000000
VERSION     DAXWLF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.219"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(141..2633)
                     /locus_tag="ACGE4U_002594"
     CDS             complement(141..2633)
                     /locus_tag="ACGE4U_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFF8394687.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2640..3107)
                     /locus_tag="ACGE4U_002595"
     CDS             complement(2640..3107)
                     /locus_tag="ACGE4U_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFF8394688.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          882 a          710 c          533 g         1132 t
ORIGIN      
        1 ttgaaaatta tttcacaaaa aatcaaaata aagttcgata tattctgtcc aaatataaaa
       61 agagattgtg acaaaaatct tgtcacaacc tcttaatcaa gtcttactat tataaaaatt
      121 cagctacaca ggccaagtaa ctatagggaa cctactgttt cctttttagg tccgcgaaca
      181 ttcaatgtga tcttaccttt tgaagcacct actgttacgt ggtcacctaa acgaatttct
      241 ccgccaagca aagcctcgct caatcgatct tcaatttctt tttgaagtgc gcgacggatt
      301 ggacgtgcac catattcagg atcaaatcca gctttcccga tcacatcgat tgcagatggt
      361 gtgattttca cttgaatatc ttgttctttc aatcgtttga taatcgcttt gctcatgatt
      421 tggacgattg tatggatttc atctttactc aatgaatgga agacgacagt ctcatcgatt
      481 cgattcaaga attcaggtcg gaaagctttc tttaactctt ctaagatacg tttttgcatg
      541 gctttgtgat ctttagtaac atcttggaca ttgaatccga catttttttc ttctcgaatt
      601 tgtgtcgctc cgatatttga agtcataatc atgattgtat ttctaaagtc cacttttcta
      661 cctttagaat ctgttaaatg accatcatcc aatacttgca gaagtaaatt gaaaacgtct
      721 ggatgtgctt tctccacttc atctaagaga atcactgaat atggtttttg acgaattttt
      781 tctgtcaact gtccaccttc atcataccct acgtatcctg gaggagaccc gattaatcgg
      841 cttgtactgt atttttccat aaattctgac atatcaacac ggatcagggc atcttcactt
      901 ccaaacatga cttctgaaag tgctttggcc aattctgttt ttccgacacc agttggtcca
      961 aggaacatga atgaaccaat tggacgatca ggatctttca atccgcttcg tgcacggcgg
     1021 atcgcacgtg aaactgcttt gacagcctca tcttgaccaa cgacacgttc atgcagaagt
     1081 ccttccagtt ctaacaagcg ttcgctttct tttttctcca actgctgtag agggacacca
     1141 gtccactccg atacaactgt agcaacatct tcttctgtta cacgatcaga ataaccttgc
     1201 tcctgttttg cttcaattgc agaaactttt tgcagctttt tacgtagtgc tttttcttgt
     1261 ctgcgtaatt gagcagcatt ttcaaagtct tgtttttgga tcgctgcttc cttttcttgt
     1321 actagttcat cgatttctag tttgatcaca gttgattttg ttaagtgatc ggtttgatcc
     1381 aaacggactt tcgctgctga ttcatcgatc aaatcaattg ctttatcggg tagttgacga
     1441 gaagtgatat aacgaacaga aagattgact gctgctcgta cagcttcatc tgtgatctcc
     1501 actccatggt gctcttcata tcttgtacgt aatccttgaa gaatcacttc tgcttcttca
     1561 ggagttggct catctacttg aatgcgagca aaacgtcgct ctaatgcaga atctttttcg
     1621 atatattttt gatattcatc tagggtcgtt gctccgatcg tttgtaattc tcctcgagct
     1681 aacgccggtt tcaagatatt cgaagcatca attgctcctt ctgctccacc tgcaccaatt
     1741 aaggtatgca attcatcaat gaataagatt acttgaccat cattatagat ttcgtcgatg
     1801 acctttttca ttcgatcttc aaattcaccg cgatatttcg taccagcaac caaagatccc
     1861 atatcaagca tcatcaggcg cttttcttgc atgtcttctg gcacttcacc taaaatgatt
     1921 ttttgagcta acccttcagc aatggctgtt ttgcctacac caggttcacc caccagtaca
     1981 gggttattct ttgtccgtcg gctaagaatc tggataagcc gtttcacttc tctcgaacga
     2041 ccaacaactg ggtccagctt gttttctttt gctaatttcg tcaaatcacg tgctaatgaa
     2101 tctagcgtag gtgttccttg cggtgtttgc cgtttatctt gtacatttac atttctacga
     2161 cgattcattc cttttccagc agaatcacca gctagcccca ttttttgttt cagaacttga
     2221 cgggcttttg aaaggctgat acctaagttg atcatgattc gagtagccat gatactttca
     2281 tcttttaata agctcatcaa aatatgttct gttccaactt tttgagaacc agttttcttc
     2341 acttcgctgc tagctaaagc aaaaacttgt ttcattcttg gtgaatatgg aagaaagact
     2401 cctttagggt aagaacggat accaccataa ccaatcagat gttcaatttc ttcataaatt
     2461 tccgtttcat tcaaatccat ttcacgtaat acttttccag caattccatt ttgctcgacc
     2521 actaaggcta gtaaaacatg ttctgaacta actgattgat gtttcaaccg ttttgcttct
     2581 tcttgtgcaa tttctaatac tgcttgggct ctttcagtaa aaagttcttc catctaatct
     2641 cacttcccat cttcataact taaacgttcc agaaatgctc ggagaatccg agcacgtgta
     2701 tgatcttcat attcattgaa attcaatgtg ttttttgcaa ttgcactcaa catcaagttt
     2761 ccttcttttt ttgtaattat ttggtcttca taaagttttt gaataatagc aaaagcattt
     2821 ttctcactga ttgtatcatc aaataactga tttatctgct caagtaattg ttttttatct
     2881 gaaatcctta cttttgcaat tcgtatataa ccaccaccac cacgtttgct ttctactaga
     2941 taaccacgtt ggatggtaaa acgggtatta atcacatagt tgatttgtga aggaacacag
     3001 ttaaagaggt ctgccatttc aatccttcga atctcgatca tttcactatt ttccagaatt
     3061 ttctttatat atgattcaat taaatccgag gtattctgat gtgccatctt attcattccc
     3121 tttgactaat attgacctta attataacga atttgataat agaaagaaag aagaacgctt
     3181 tttgttaaat aaatccttta gaatcaacaa aaaaaaatac ggaatatact attccgtatc
     3241 gacgcgccca agaggag
//
LOCUS       DAXWLF010000216         3127 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXWLF010000216 DAXWLF010000000
VERSION     DAXWLF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.220"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(17..715)
                     /locus_tag="ACGE4U_002596"
                     /pseudo
     CDS             complement(17..715)
                     /locus_tag="ACGE4U_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            794..970
                     /locus_tag="ACGE4U_002597"
                     /pseudo
     CDS             794..970
                     /locus_tag="ACGE4U_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1146..1715)
                     /locus_tag="ACGE4U_002598"
     CDS             complement(1146..1715)
                     /locus_tag="ACGE4U_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8394689.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1789..3078)
                     /locus_tag="ACGE4U_002599"
     CDS             complement(1789..3078)
                     /locus_tag="ACGE4U_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394690.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
BASE COUNT          864 a          627 c          550 g         1086 t
ORIGIN      
        1 aagagggggg gattttttat cgttgtggaa tatttaaata ccaacgcttg tcatgaaaat
       61 cttgtgctcc acccttagta tttccttctg gatcgtttgt cgcacgcatc atgacgtata
      121 ctttcttatt agggaaatca cgcatattga aagatacatg atagccaaca tttcctaaag
      181 tattataagc ttgatttacg tctggtctat aaatcccatc agctcttact ctagctagtt
      241 cttttccagt attgtagtcc ataatgaaaa tatactcgta tttataatta gcaatgtgcc
      301 atccagctac atgcaagttt gcgttttcga tttctccgaa ctgatcaatg tgggcgtaat
      361 ttgttccatc tgtcagcgta gaatttgcag cacctgctcg agttggatca attactggtt
      421 tatcctccga agtagttgga ttatcatccg taaaaccatg agccaaatca taggctagtt
      481 tttctttact tacacccatt tcagaaagat aaccgtaagg atctgtatga tcgccccaaa
      541 tattttgtgt tacccataaa tgcgatttga ttcctggttg gttataagga gtgtctaatg
      601 ttaatggaat accatatttc attgctgaat ctcttgccaa ttcaacgtat actttatagt
      661 tcttttcaaa agtttcttta tcatgtgtgt gttgtaactc gatttgcaca ggacttttgg
      721 ctcacgaggt tgtcctactc tacatcttgc tgatagatat gctgaaagga aaacaccaac
      781 attatgtttt gctttttcag ctagaacgag acaagcagga atacctttag aagatatcaa
      841 agatttttta ggtcataaag atgtttccac tacacaagtc tatgcccata tttctcccga
      901 agtgaaaaaa agatcaatga atcaactaga aaactatata gaagagcaaa taaaaaagca
      961 ctcaaattga gtgctttttt aagctcacta tcactaaaac tatcactaat tttatttata
     1021 agcgaacatg tttctatcac tgatttttta aatctagcga aaaaccctta tcttaaaggc
     1081 gttcgttcaa ttctttggca aggtcttcaa agcctggctt gcctagaagt gcgaacatgg
     1141 ttttcttata gctcatgtag aaaaattttc ttgatgatga ttttatccat caattgagat
     1201 tgactgtttt gctctcctat gatttcagct atgattgtcc gattttgcgg ttttcttctt
     1261 aactggtagt tgattttccc agtttccatc aactcgctaa gcatatccac gactccgcca
     1321 aatccggtct ttttcgtaat aaaacctagt gctaccattc gttcatctag attttttgct
     1381 gtaatcgtca caccagtaat gagcgagtgc tgtaatgttt gggccagctc ttccatccct
     1441 tcagcattgg atacgacgga agctgttttg ttaataggtt cttcgtttat ttcttttggt
     1501 tctttcctct ccactttcac tgtttgacga aggacatcta gttcttgcaa gagcgttttg
     1561 tattgttctt gggtcagcat cacgccagct actttttcac gattaaatat atatacgcca
     1621 gttcccgcgt ctcgcgcttg agcaaataca tccatcggcg atcgtttcac ttctgtgatc
     1681 gaagatgtgg gaacttctaa tttctttaat tccaatggat ttcctccttt actaatcgct
     1741 ttttattata gcacagactg acttgttttg atcttttttc caaaggtttt atcggttttt
     1801 gcctaaccag taattgatat cggtaagaag tctttcgata ccaaatgaag gatggtacag
     1861 atacgttttt tgatcttcgt tatggtctag tggaaaaaaa tgatcggtga tcactagatc
     1921 tgcataccga ggcggcttat ttcgagtaca tacatgtata gagatccgtt ttcgatgtct
     1981 tagcgttttg ttcagtaccc cagcaagtct tcttgcttct tggtgttcat agcgaaaaaa
     2041 aaagacgatt ttcacttcag gagggtggac atctaaatac cctcgttcct ggagtgtttt
     2101 cagtaaaagc atcccccact ctactgcggg gtgtgcaagt gcttgttgga tcgctagccg
     2161 aaattgatat agtttatctt tggaattggg aaatttttct aatgtttcct gataataacg
     2221 aacttcttct atcagccggt catggatacc aattacgtaa cgaagccatt cttggtaaag
     2281 tacttttatc agctttgatt tgctttcgct ctcaaattcg ctattcagcc aaaacagcga
     2341 atcaagcttt tcagtcacaa tcgtcaattt ccgatagtct tgtgtctctt ctatccgttt
     2401 ccaccgcttt tcagtaatct ggttacagct caagcgcaaa attgctagat aatttttttc
     2461 ttttattggc aaaatctgct cctctctaaa tagcaattca ggaaacaatt caaagaatag
     2521 tgttcgactg tctcctaaaa tctcgatttc tgccggatcc tttctttgct tcaataattg
     2581 gatgaacatc tcagaaaagg actgctctcc atcatctgca aaaaaatgcg gaaaatcttt
     2641 caaaaagtaa aacagcagat atcttacttt tctttctgaa ccaaagatct gtgctttttt
     2701 ataagaaaaa gaaaggtggt actcgccaaa ccactttctc aatcgctctt gcgtagaacg
     2761 atcgtacttc tgctcgtaag gttcatttaa tagcagattt ttcagtaaac aaaacggggg
     2821 tgattggatc agaaaaacaa acaagagata aggttcgcta tatccttgat cataaactaa
     2881 ctgaacggtt ttcttatgaa gaatcagctg aatgggacag tcgctttccg ccaaacgttt
     2941 cagatgccga tccactgttt taacatcgat tttcagttct tcagctaaat cagcttttcg
     3001 ccagatcaaa tctttcatta gattgattag tgaaaataaa ttctgtgcat tttttgataa
     3061 tagtttttgc catctcatcg atcatttctc acctgatttc taaacatatt gtttgatcaa
     3121 aatgagt
//
LOCUS       DAXWLF010000217         2992 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXWLF010000217 DAXWLF010000000
VERSION     DAXWLF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.221"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            109..465
                     /locus_tag="ACGE4U_002600"
     CDS             109..465
                     /locus_tag="ACGE4U_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8394691.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(706..1710)
                     /locus_tag="ACGE4U_002601"
     CDS             complement(706..1710)
                     /locus_tag="ACGE4U_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFF8394692.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            1876..2727
                     /locus_tag="ACGE4U_002602"
     CDS             1876..2727
                     /locus_tag="ACGE4U_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8394693.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2744..2989
                     /locus_tag="ACGE4U_002603"
     CDS             2744..2989
                     /locus_tag="ACGE4U_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394694.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT          963 a          575 c          531 g          923 t
ORIGIN      
        1 gaagcaataa gaacaaaatg aggaaaagtt taaaattcag ctgttgccaa caactttcaa
       61 tagacgaaat cttctatttc gttaataata aggaaagagg tgaagtacat gaaaaaatta
      121 attggcgtac ttgtcgtttt aggcatattt ctcggcggaa gttttgcagc ttatcattat
      181 ttttacggcg gcgaagctta ttatacaaaa atcacgacga atggcgaaat gtcagctacc
      241 caagcagata acggagagaa attcacgact tatacctaca agcaagcggc ttataataaa
      301 gatggtgaaa aaaaacaggt gacgcttcgc gaagaaagag aacatccatt aaagatgaat
      361 gcgtatctta aactcaaagt caatcctcgt aaaggtgtat taagctggga agaaataaag
      421 gcttctgaag tgcctaaaaa tgcggcagaa aaaatcgata attagtaaat ggtcttaaaa
      481 ttttattaga taaaaagagg ctgggacaga agcgtttaac tccaagaaat aagaaggagt
      541 tgcttctgct tccgccgttt atacgtgttt agagcgtgag acaaaagtgt tctttacttt
      601 tgtctcacgc tctttttttg ttatattcgt cttttccgat ccttctcttg acacaattca
      661 tttaactgat cactggtact tgaacaccta atattctagt catttttata aacgattcaa
      721 tacttcatcc acatttttga tcatcacgga tatcgtacca tccggattat tcacaaattc
      781 aatcaaatct gggttgcgat atacatctac tggtacgatc agttcgatcc cgtttgatag
      841 cttgaatttt tgttttccaa attttttctc agaaatttct tgtacttctt ttacaggagg
      901 tgcttctggg acaaatcctt gctcttgtac ttcttcttta aaagccattt ttgccgtgat
      961 gttttccttg aagacttttt ccgcaatcat ctcatgatcc aaccgacctt tttcttctat
     1021 cgtatcatag acagcttctt tcaaatcagc catgatatca aattcttctg tctcaaattt
     1081 ttttccaaga tgcttggcta ctttttggat cacttttaca ttttcttcta aagaaggtgc
     1141 aggaacactt tcgatcactc ttccagaaaa ataaagctct tttttgccag aaaactcata
     1201 ttttttttca attaattcgt aggtccaatc agtcaaattc acagcgaatg cttcatctgc
     1261 tttttgtgtt ttcgccccta gaattgctcg attcaaaatc aagcggttgt cgatccctgc
     1321 ttcgtctgct tcaacaaagt gagtgtaagc ctcacgataa ttgactttca ataaagataa
     1381 gtacatcact gtatctaatt catacaagac aacaaataca tctgcactag gtgcttcttc
     1441 actttcttgg tagatatcga accaccgctg aacgattgct tcacttgctt ccacaaaatt
     1501 ttcttgagcg attttaagtg attcaacgat catgctttct tctgctagtt gtccagtttt
     1561 tgtttgagca gaagagattt tttggatctt cttttgaaga aaatcacgaa tatattcttt
     1621 tgtcagatcc agctcttttt gtgaaaagac tggagaacca gtttctctgt caataatatg
     1681 cagaattgct ttttttaaat agatatccat tttctcattc ccttctgtct tagtttacgg
     1741 aaaaaaaatc aaaaatacca gaatcaaaat cattttcttt taaacaaaaa tttcaagagt
     1801 agaaacaaaa tggattcttt ttttcgaaaa atgcgctaca cttatagttg aacgaataaa
     1861 aaaggagctg aaagtatgaa ttcattaaca agcacttatc gtctatcaaa tggttacgaa
     1921 atcccggtag taggattcgg cacatggcaa acacctgatg gggatgtcgc tgtttcctct
     1981 gttaaagaag cgctggctgc aggttatcgc catatcgata ctgcccaagg atacaagaat
     2041 gaagaaagcg tcggccaagc gattaaggaa agtggaattc ctagagaaga gatattcttg
     2101 acaacaaaat tatggaatgc aaaccacagc tatgaacttg tgatgtcttc ttttgaagaa
     2161 tcattgaaga agctccaaac agattattta gatctgtttt tgatccactg gccaaaccct
     2221 gttgcttttc gtgataattg ggaacaagca aatgcagata catggcgagc atttgaagaa
     2281 ctgtatgaag caggtaaaat caaagcaatc ggtgtgagca atttcttacc ccaccatcta
     2341 gatacactag caaaaacagc aaaaattatg ccgatggtca atcaagtttt ccttgcacca
     2401 ggcgaattac agacaactgt agttgagtat gcaaaaaaac atgatatgat cctcgaagca
     2461 tatagtccat taggcactgg taaaatcttc gatgtgcctg aaatgaaaca aatcgctgaa
     2521 gcacatgaca agacgattgc acaagttgct ttgcgttggt cgctgcaaca cgaattcctt
     2581 cctcttccta agtcagtgac accaagcaga atcaaagaaa atactgaatt atttgatttt
     2641 gaattaacag aagaagaaat gaaacaaatc gatcagttag atggtgtagt cggcaaagca
     2701 aaagacccag atactaccca attttaagtc gaaaggtgat tttatggatc caatcgaacg
     2761 cttgaattct ctctcggaag aagttaccca gacttttcat tccgattttg tctttttgat
     2821 cgatgcagag aaaatccaac attttcctgc aagaaactgg acacatgatc aaatcatcga
     2881 agaactgaaa aaacggttcg atcattcgct aatggtcaaa ccctggcacg aacatgaagt
     2941 catttattca cctgaactac ctgtttttgc cttgataccg aaaaaataga aa
//
LOCUS       DAXWLF010000218         2969 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXWLF010000218 DAXWLF010000000
VERSION     DAXWLF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.222"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(965..1483)
                     /locus_tag="ACGE4U_002604"
     CDS             complement(965..1483)
                     /locus_tag="ACGE4U_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFF8394695.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            1850..2098
                     /locus_tag="ACGE4U_002605"
     CDS             1850..2098
                     /locus_tag="ACGE4U_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394696.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            2601..2969
                     /locus_tag="ACGE4U_002606"
                     /pseudo
     CDS             2601..2969
                     /locus_tag="ACGE4U_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          999 a          530 c          447 g          993 t
ORIGIN      
        1 tttacttggt atatttatga ataatcgcaa atttacccct atactactag tttgtaagtt
       61 agattaataa aaatactaaa gttatttgtt cttttattaa atacacctac aacttataaa
      121 ttgatggtat ttagataaca cgaaagaata aaaataacag ctgatagtaa agtttgttga
      181 cgattgctac tcaatcgaaa gtgagttagt gcgtatgaac ttgcttctga aaacttatga
      241 aatgaaagac cttttacatc ttgcagaaat cgttactggg attaaactaa gcaataaaaa
      301 ctaatgttcc cctcattctt tgaacttcta ctatttgatt tatttattct gataaatgac
      361 gatttcacct tcaggatctt caaatcccgt atagaattta tttaagatct cttcagtatc
      421 agtaatagga taggcgatag gccacatacc ctttgatggt tagaataaac tgctcaaaat
      481 ctgtatcgta tgttggcttt agcatattaa caaaggctat ctccaaatta taaaccataa
      541 gcgttgaatc gttcttaaca atttcaattc ttcctaaccc aaagtcagaa gatataatta
      601 ccggacttgc cttacccttc atgtgggttg ttgagattcc atatttaaag ggaattgtcg
      661 gtactatcgg cggtctgaaa aacatcccaa aaataaaaaa atctttcccc tgctagtctc
      721 ctaagtccct ttgtagcgga tatggcacca taatctcatt ttatcgaagt gagtcatggt
      781 ttatctgttg gccaaccaaa attgatacaa tctaacgatg caccctctac ggcattctag
      841 gggagtgcac ttgcattcac ccaataaaaa ccggcagtta gcaaagaact tatgtagtcg
      901 tatgttctta ctgttaacat acaagaggtc tcctttccac aattgcttag cagtaaacgg
      961 gaatttacaa agctaatctc ttccattttc ttatctttgt tctaaaagtc ccaaatggag
     1021 caactgtatt tacatgaata aatttatata cttcccatac agccgttttg gttgcctcgt
     1081 ctgcccattt tcttttgtgt gcagtgaata gatcttcatc actcattgaa tcaatcatca
     1141 tgttaatatc atcaatatta cgattgagaa tttcctctaa ttctttcaat ggtagatgtg
     1201 cataggtatt tgtaaaccac ttatataact ctcctaactg attccattta aattgatcag
     1261 aaggcgtttt aacagataat ccatttcttt catcattttc ccattttaga attaaattcg
     1321 tccaaccaac ttgatatgct aaattttcag caggtgttct atccacttcc tcaactcttt
     1381 tatccttcaa ttcttcagga atagtttcaa attctaaaat atatttagta taagtcctat
     1441 ttatttcttt ttttaattct tctttatttt tatatgttct cattatatta cctccataaa
     1501 ttcctaattg tctcctttaa ttcatctata atttcatcca cgatatttta atccgttcta
     1561 caatgcatat ctgatatgca ttgactgtct aactcatatg tgaatgattt ataaaatatc
     1621 ccaacgtctc atctaaagaa atgttgatcg tcagcaagga gctaaaaaat acgaatatat
     1681 ttcttttgta gtaaaatcgg actggtttgc atatcatttt cttcgtaaat atcaaaatca
     1741 tgttaactac tatcaattac taataaaaac taccacttta agcgttaaga actgttaagt
     1801 ttagttaagt gaggatagtt cacaaaatcc attgttaaac ttcacgaaag tggtaaaact
     1861 caaaatgctt tatataggat tacagcatcg ctctttcttc gattgcgcgt tgggtaaaaa
     1921 atattatgaa gtaaaaattg acgatgacac gttccttacg gctcatcaga tgaaacagct
     1981 acaaaaacga aatgatcaac tcgaagaaaa tttaattcta aaaaagcgat tgccatattc
     2041 acgcctcgct cgacgaacta ttaaaggtca agatttacct gaagatcatc ctttttagga
     2101 aattgaagct ttttatgttg cacaatgcat ttataatact acaatgatgt tttcaccaga
     2161 tattcttcta gggtgcggtg taatgaaaca gatacacttg caaaagaaaa ttcaccgcga
     2221 atttactaaa attatgaata attatgttaa tcttccgaaa tttttttcca aaattaaggg
     2281 atcaagctgg tgttatagat tgtttaaggt tggcaaaaaa ataacttctt tttaaacaat
     2341 tatagaaaac cgatactctg ttaagttggt tgacacggtg tcgattttta tgactttctt
     2401 attctgaaac ttcactaacg gacagtacct aaaaagtcta tagtatggac gtttacgagt
     2461 actattttat caaaaatgct gctcatttca acttatatgt tttagtgaac tacccccact
     2521 tagcttggat aggctacaat taactagaca gaaaaattaa ggtgtgtaga ctagaagaaa
     2581 acataccagg aggaattttt atgtctaaga gaacacgaag aactttttca caagaattca
     2641 agcaacaaat cgtcaatctt tacttagctg gaaagccacg tgtagaaatc attcgagaat
     2701 atgaactaac ggcttcagca tttgacaaat gggtaaagca atctaaaacg agtggttcat
     2761 tcaaagaaaa agataatctt acgcctgaac aaaaagaatt gttagaacta cgtaaaagaa
     2821 accagcaatt agaaatggaa aatgatattt taaagcaagc agcgctgata ttcggacgaa
     2881 gagacaagta atcgatgcga ataagcatct ttaccctata tcagcgatgt gcagaatatt
     2941 aggtctatca cgtcagtcct attattatc
//
LOCUS       DAXWLF010000219         2783 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXWLF010000219 DAXWLF010000000
VERSION     DAXWLF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.223"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..359
                     /locus_tag="ACGE4U_002607"
     CDS             <1..359
                     /locus_tag="ACGE4U_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFF8394697.1"
                     /translation="SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGA
                     GMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVI
                     SWLVTLNVYKHSQRDF"
     gene            923..2185
                     /gene="mobV"
                     /locus_tag="ACGE4U_002608"
     CDS             923..2185
                     /gene="mobV"
                     /locus_tag="ACGE4U_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFF8394698.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            2430..>2783
                     /locus_tag="ACGE4U_002609"
     CDS             2430..>2783
                     /locus_tag="ACGE4U_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFF8394699.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFL"
BASE COUNT         1003 a          378 c          583 g          819 t
ORIGIN      
        1 cttcctttct tttagaaaca acatcatggt tcatgacaat tataatcgta tttgttttag
       61 gtgggctttc gttcaccaaa acagttatat caacaattgt ttcaagtagc ttgaaacagc
      121 aggaagctgg tgctggaatg agtttgctta actttaccag ctttttatca gagggaacag
      181 gtattgcaat tgtaggtggt ttattatcca tacccttact tgatcaaagg ttgttaccta
      241 tggaagttga tcagtcaact tatctgtata gtaatttgtt attacttttt tcaggaatca
      301 ttgtcattag ttggctggtt accttgaatg tatataaaca ttctcaaagg gatttctaaa
      361 tcgttaaggg atcaactttg ggagagagtt caaaattgat ccttttttta taacaggaat
      421 tcaaatcttt ttgttccatt aaagggcgcg attgctgaat aaaagatacg agagacctct
      481 cttgtatctt ttttattttg agtggttttg tccgttacac tagaaaaccg aaagacaata
      541 aaaattttat tcttgctgag tctggctttc ggtaagctag acaaaacgga caaaataaaa
      601 attggcaagg gtttaaaggt ggagattttt tgagtgatct tctcaaaaaa tactacctgt
      661 cccttgctga tttttaaacg agcacgagag caaaaccccc ctttgctgag gtggcagagg
      721 gcaggttttt ttgtttcttt tttctcgtaa aaaaaagaaa ggtcttaaag gttttatggt
      781 tttggtcggc actgccgaca gcctcgcaga gcacacactt tatgaatata aagtatagtg
      841 tgttatactt tacttggaag tggttgccgg aaagagcgaa aatgcctcac atttgtgcca
      901 cctaaaaagg agcgatttac atatgagtta tgcagtttgt agaatgcaaa aagtgaaatc
      961 agctggacta aaaggcatgc aatttcataa tcaaagagag cgaaaaagta gaacgaatga
     1021 tgatattgac catgagcgaa cacgtgaaaa ttatgatttg aaaaatgata aaaatattga
     1081 ttacaacgaa cgtgtcaaag aaattattga atcacaaaaa acaggtacaa gaaaaacgag
     1141 gaaagatgct gttcttgtaa atgagttgct agtaacatct gaccgagatt tttttgagca
     1201 actggatcca ggagaacaaa aacgattttt tgaggaaagt tataaattat tttccgaacg
     1261 atatggcaag caaaatattg cttatgcaac agttcataat gatgagcaaa cccctcacat
     1321 gcatttaggt gttgtgccta tgcgtgatgg aaaactgcaa ggaaaaaatg tgtttaatcg
     1381 tcaagaactg ttatggctac aagataaatt ccccgagcat atgaaaaaac agggttttga
     1441 gttgaagcgt ggtgaacgtg gctctgaccg taaacatatt gagacagcta aatttaaaaa
     1501 acaaactttg gaaaaagaga ttgattttct agaaaaaaat ttagcagtta aaaaagatga
     1561 atggactgct tatagcgata aagttaaatc agatttagaa gtaccagcga aacgacacat
     1621 gaaaagtgtt gaagtgccaa cgggtgaaaa gtccatgttt ggtttgggaa aagaaataat
     1681 gaaaacagaa aagaaaccaa ccaaaaatgt tgttatatcg gagcgtgatt ataaaaactt
     1741 agtgactgct gcgagagata acgataggtt aaaacagcat gttagaaatc tcatgagtac
     1801 tgatatggcg agagaatata aaaaattaag taaagaacat gggcaagtta aagaaaaata
     1861 tagtggtctt gtagagcgat ttaatgaaaa tgtaaatgat tataatgagt tgcttgaaga
     1921 aaacaagtct ttaaagtcta aaataagcga tttaaagcgt gatgtgagtt taatctatga
     1981 aagcactaag gaattcctta aggaacgtac agacggctta aaagccttta aaaacgtttt
     2041 taaggggttt gtagacaagg taaaggataa aacagcacaa ttccaagaaa aacacgattt
     2101 agaacctaaa aagaacgaat ttgaactaac tcataaccga gaggtaaaaa aagaacgaag
     2161 tcgagatcag ggaatgagtt tataaaataa aaaaagcacc tgaaaaggtg tctttttttg
     2221 atggttttga acttgttctt tcttatcttg atatatatag aaataacgtc atttttattt
     2281 tagttgctga aaggtgcgtt gaagtgttgg tatgtatgtg ttttaaagta ttgaaaaccc
     2341 ttaaaattgg ttgcacagaa aaaccccatc tgttaaagtt ataagtgacc aaacaaataa
     2401 ctaaatagat gggggtttct tttaatatta tgtgtcctaa tagtagcatt tattcagatg
     2461 aaaaatcaag ggttttagtg gacaagacaa aaagtggaaa agtgagacca tggagagaaa
     2521 agaaaatcgc taatgttgat tactttgaac ttctgcatat tcttgaattt aaaaaggctg
     2581 aaagagtaaa agattgtgct gaaatattag agtataaaca aaatcgtgaa acaggcgaaa
     2641 gaaagttgta tcgagtgtgg ttttgtaaat ccaggctttg tccaatgtgc aactggagga
     2701 gagcaatgaa acatggcatt cagtcacaaa aggttgttgc tgaagttatt aaacaaaagc
     2761 caacagttcg ttggttgttt ctc
//
LOCUS       DAXWLF010000220         2759 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXWLF010000220 DAXWLF010000000
VERSION     DAXWLF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.224"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            382..570
                     /locus_tag="ACGE4U_002610"
     CDS             382..570
                     /locus_tag="ACGE4U_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFF8394700.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            648..1661
                     /locus_tag="ACGE4U_002611"
     CDS             648..1661
                     /locus_tag="ACGE4U_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8394701.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1728..>2759)
                     /locus_tag="ACGE4U_002612"
     CDS             complement(1728..>2759)
                     /locus_tag="ACGE4U_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8394702.1"
                     /translation="VGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAAL
                     DYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYNL
                     SDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLER
                     MTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKYM
                     SYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIVV
                     ASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVRE
                     SFIPRTKRY"
BASE COUNT          850 a          529 c          544 g          836 t
ORIGIN      
        1 gttagtctac attattttgg cgatttgcca ataaggactg ttgagttgtg caaaatcaaa
       61 tctgggtcag aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact
      121 gattgatcaa aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg
      181 tttgacgcgg aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga
      241 cccattattt tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg
      301 ctgggagttt tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg
      361 actgtaacga aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa
      421 gcgacacgat atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca
      481 acagaagaag aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca
      541 ttgcagaaga agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac
      601 actatggtat aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa
      661 ttcttaaatt tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc
      721 ttgccttcca gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata
      781 ctgttcaaat cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa
      841 cagatttgac gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa
      901 acagcaaggg gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag
      961 ataaatcggg tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg
     1021 ttgcgatgaa ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga
     1081 atgaactgat caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac
     1141 aattattttt tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac
     1201 aaacgaatga tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc
     1261 tgaaaggaac catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt
     1321 cggatgatcg atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa
     1381 aagattggaa aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg
     1441 aatttccgcc aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag
     1501 cattagaaaa tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata
     1561 caaaaaaaga gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt
     1621 gttaccagca gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt
     1681 ttcgttttga gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt
     1741 gttcttggga taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt
     1801 tcgtcccgta taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat
     1861 ttcgcattga tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta
     1921 tcaaagcttg ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga
     1981 tagacaccaa ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca
     2041 ttttctaata gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga
     2101 taagacatat atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc
     2161 ttttcattta tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg
     2221 atataggcta ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc
     2281 cgttcaagat ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg
     2341 actatttgca tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca
     2401 acgatcaccg cacctaactg atctagatct tcccattgtt taggattatc agatagatta
     2461 tagattcgat tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa
     2521 tgtttgtaat agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc
     2581 gaaggcattc tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct
     2641 gcctctacta tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac
     2701 acacgggaaa ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaac
//
LOCUS       DAXWLF010000221         2714 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXWLF010000221 DAXWLF010000000
VERSION     DAXWLF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.226"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(40..1416)
                     /locus_tag="ACGE4U_002613"
     CDS             complement(40..1416)
                     /locus_tag="ACGE4U_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFF8394703.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1544..2632)
                     /locus_tag="ACGE4U_002614"
     CDS             complement(1544..2632)
                     /locus_tag="ACGE4U_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8394704.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT          853 a          556 c          436 g          869 t
ORIGIN      
        1 tttattcaag caacacgtct ttcataacca agtttccaat tatcttcttg gctcgtcatc
       61 ttcgaattct tttctgaagt atcttcgttc ttcttcaggt tccgcttctg tttcttccgg
      121 atcatgaaaa atgacacgga ttttaaggat tcgagaacct tccatttctt cagaaaccag
      181 cgtaatatta tcgacatcaa acgataactt ttcgccttca tccggaatca tgcctaatgc
      241 tgtaatcaaa taaccagcca tcgtatctac atcactcata tgcagatcac tgtcaaaagc
      301 ttcattgaac tcatcaatca gcatacgtcc ttggatgata tattcgtgtt catcgatttt
      361 ttcataaaga ttctcaactt catctgattc atcatcgatt tctccgacga tttcttctaa
      421 aaggtcttct agtgttgcta acccaacgac gccgccatat tcatctaata aaatagccat
      481 ctgattctgc gtctttttca tttcatataa cagatcatca ataaagaccg tttcaggaac
      541 gaataaaggc tcctgcatga ttttcttgat atctaaatta tcaaaaccga atttatgggc
      601 agcttttagt aaattctttg tatgaaggat tccgactact ttatctttgt cttcgttata
      661 aacagggatt cttgaatagt tttcggataa aacttcattc acattttctt ccacagtgtc
      721 gttgatatca atcataaatg catccgttcg tgggaccatg acttctcggg caactttcgt
      781 atcaagagaa aaaacccctt ggagcatttc taattcttca ttattcaata cgccttcgtt
      841 ttcaagcata taacgcattt catctctggt catttttgaa tcttcatcat caaaagtcat
      901 cggcgtgata cgcgccaata aacttgtgga agcagaaagc agccagacga agggcttagc
      961 tactacacca atcaagcgta tcagccctga agtgaacttt gcgacttctt ctgatttatt
     1021 taaagcaata cgtttagggt acaattctcc aaaaacaatc gatacatagg tcaaaatagc
     1081 cagcacaatg atatttgcaa tattttttgc cgctgtgccg ccacctaata ccggagctaa
     1141 ccgttccgat aaagtattag ctagtgaagc accagacaaa atgttgacca aagtgattcc
     1201 tacttggatt gtcgatagga aattatttgg attctgcaat atttttaaaa gcttttgaga
     1261 tttcacatct ccttcatcag ctttttgctc gattcgattc ttattgatcg aaacaacagc
     1321 aatttcggaa gctgctaaaa aagcattgat caatgtcaaa acgatcaata ataaaatctg
     1381 cgctaacagc gactgactct cagggtcagc attcataatc gcattctctc cttataaaat
     1441 taatttttag tttacgatag aaaaaaacgt ggcactatag ccacgttaaa atggtatcac
     1501 aaagcaattt tctttgcaat aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa
     1561 ttttcttctt gttttcattt tgcttgtctt ctttgacgat tttgattata taggaaatga
     1621 tcgtcttgca gattgcgtaa aaaggaacac ctaagaagat accaagcaat ccggcaatat
     1681 ttccagctac tagaagaacg ataatgatcg ttaatggatg gattttcagt gatttcccaa
     1741 ttacattagg ataaatgata ttcccatcca actgttggac gattagcaca accacacaac
     1801 aaagtaatgc ttttaccgga tcattgaaga ctgttactag aaatgcaggt gctagtccta
     1861 gataaggtcc taaatatgga attaggtttg tcaatcccgc aatgacccca aataagaaag
     1921 cataatccac accaattacc atatatccta aaaatgtgaa ggtaccaacg aataaacatt
     1981 cgattgcttg tccgctaatg taattagcca atgttttatt cagttgcccc aataactcca
     2041 caatttggtt tcttcttttt tctgggaaaa actgttggat gtttggcact agtttttctc
     2101 cgtctttcaa catatagaac aagatgaatg gtgctgtaat gattactacg gttgccgaag
     2161 cgatcgttcc aacgatcgaa cctaaactat tggatactcc actaaggaat tgctgaatga
     2221 ttgtcccgta agaaatgtct aattgctcaa aatatttatt gatatctact tccttgaaca
     2281 aaggcaattc ggctacctct tttaaccagc tttctacctg tttgatgaaa tcaggcatat
     2341 tagatgccaa tgaggaaatt tgctgtacta gacttgggat gacactaagt aaaatccaaa
     2401 taattgctgc aactaaaagg agcagcacaa ttgcgacagc ccatattctt ttgatttttg
     2461 ctttcatcaa cagcttgaca ataggattca ataaatagta aagaaagcct gcaatcagta
     2521 caggtgcaaa taacgtggaa aaaaatgtcc cgatcggttc gaaaataaag tttatttctg
     2581 atgaaatcat gatcaatgca gctatgatca ataattcaag cgaccaaaac attagtttag
     2641 actgtctaag tttttcaaac aatagatttc ctcctttctt tcttactagt atatattata
     2701 ttcgttaaaa attc
//
LOCUS       DAXWLF010000222         2571 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXWLF010000222 DAXWLF010000000
VERSION     DAXWLF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.227"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(69..971)
                     /locus_tag="ACGE4U_002615"
     CDS             complement(69..971)
                     /locus_tag="ACGE4U_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8394705.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(968..1660)
                     /locus_tag="ACGE4U_002616"
     CDS             complement(968..1660)
                     /locus_tag="ACGE4U_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFF8394706.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(1703..2344)
                     /locus_tag="ACGE4U_002617"
     CDS             complement(1703..2344)
                     /locus_tag="ACGE4U_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFF8394707.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(2382..>2571)
                     /locus_tag="ACGE4U_002618"
     CDS             complement(2382..>2571)
                     /locus_tag="ACGE4U_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394708.1"
                     /translation="NFYRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGA
                     VSKMVKSGNVANISYLMI"
BASE COUNT          711 a          573 c          423 g          864 t
ORIGIN      
        1 aaaattctgt cacaacccct cttttttgat caacggtgtt caataaacag cgttctcatc
       61 tgcttttctt agctctgctg gtccagtaac cgagtaagct ctttgataaa tctttgagcg
      121 atttcactga gcggacgttt cgccgaataa atgatataaa attcatttag ttcgccttct
      181 gccaaaggga ttaaccgcaa gtgctcgcga atccctccaa tcacaatacc agatcctgaa
      241 gcgtaagcat cagtttcaag aacgatattc atcaaagtac ctcgatcact cgtataaatc
      301 actgtttctt gatcagggat ctcgataagg tcttcataaa aatacgggaa attatttcca
      361 tcctgtgtaa aacgaacttg aggataagat aacaattctt ctttggtgat ctctgcgtgt
      421 tcagctaatg gatgtccttg ccttaaaaaa atatgcgttt tgaattttcc aatgctgcgg
      481 ccacttagct ctccttgttg taaatatctt ctgataccag cttgattatg ctcattcata
      541 taaataatgc cgatctcact gtgctgctct ttgacttctt cgatcactct cgctgttgtc
      601 gtctccatga tccgaaattg tttgatctcg tctttgaatt cctcgagtag ctgtgcggtc
      661 aaaggaccta agaaatcata atgctgggca gcgatcgaaa aattgtctct cttttcactc
      721 gttagatagt gagtttctaa tgtatcaagc tggaccaata ttttctcggc acgatttaag
      781 aagtcttgcc cttcttctgt taaatatgcc cctcgattcg ttcgagtaaa cagcgtgatc
      841 cctaactcgg tttctaattc tttgatacca gctgaaagac ttggctggga gacaaacatt
      901 ttctttgccg cttctctgaa actaccattg tttgctacag cgaccacgta tttcatctgc
      961 tgaatattca ttgctcaaac tcctcgaaat aataaatttc tcttccattc aagggctgga
     1021 ttggtcggtt gttcggtcga gcatactgac catcgaaggg gagaagaaac tcttggttca
     1081 gttttcctac atgatccatt acaaaccatt ggattggccc gctggtaggc ggagttgtca
     1141 atgagccaat ataatggaaa tggctttttt gctccggaaa gaacacatcc ggatttaccc
     1201 aatgctcatg tttttctaag ttccaacttt ccccattttc ttgattatac agccaacctt
     1261 cctgtaccat tagctggtac atgacaccca caactagatt ctcttttttg ctgttcatat
     1321 gaacaaaatg aaattcaata tcttcctgtt ttccgttgat gatatgttca gaaggcaagt
     1381 ggaaatgaat atctgtcaaa taatagcgtt ctttcttgaa ctctacataa ctgagttggt
     1441 caaaaggtac taaatgaatc gtgtttttga attccttgtc agtaaaccgt tgcttttgat
     1501 aatgaaagga aagcgtgtct ccttctattt tggtttcttc ccccttggtc aaatgaatag
     1561 gcgactgcaa tggaaattcc gcacctctcg caaaccaatc acataaactc gcccaatttt
     1621 ccggaccttt gtctccagaa tagccccatt cgacatccat attctttttc aactatactt
     1681 ccccctgcat ctcatatcat ttttattttt cttctttctt tacaatcgtt gagacggttt
     1741 ctaaaaacgc ctcttttcca taaaattcct ggttcaatag ttccggaaat atctctttaa
     1801 agaaatactg aacagaaggc cgatgttcaa agcttttaat cgtagtcact gcctcaatga
     1861 acatctcctt ataaactttt tccgggttat ttttgatgat ttcatcaaaa gattcataaa
     1921 gaaggtcaac cttatctgaa atggctaata gttctccttc tacggtttca tcttttcctt
     1981 cgaacaaccg acggcgataa acttcttgga actcagctgg aatctcttgg ttgatgaatt
     2041 cgtccgtcat cttttcttca actgtttgca gcatccctct taactctttg cttgcgtatt
     2101 tcaccggcgt tttgatatca ccaataaaac gctctgtgta gtcatgattc aatgattttt
     2161 catagagcag tttccaatcg atcggttgtc cattcgcttc ttcaatgtct cctagaactt
     2221 gcgcaataga tgctacacga tacgaatgtg ctgcaactgt atgctctgta tatttaaaaa
     2281 atccaggtgc ccttgtgatc gtctctaaat tgttcaatcc taaaataaat tcattcaacc
     2341 ccataaaagt tacctccctt tgagatttct ttttccgaat atcatatcat taaataactg
     2401 atattcgcaa catttccgct ctttaccatt tttgaaacag ctcccttacc aaaaaataga
     2461 gaaaatctat atataataga aaaaagagaa gagattattc tcttctttct gctaaccagt
     2521 cttagaaaaa tgaattttaa aaaaatcaaa aactgatcac gatagaagtt t
//
LOCUS       DAXWLF010000223         2474 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXWLF010000223 DAXWLF010000000
VERSION     DAXWLF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.229"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..418
                     /locus_tag="ACGE4U_002619"
     CDS             <1..418
                     /locus_tag="ACGE4U_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8394709.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            632..1327
                     /gene="vanR-A"
                     /locus_tag="ACGE4U_002620"
     CDS             632..1327
                     /gene="vanR-A"
                     /locus_tag="ACGE4U_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFF8394710.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1305..2459
                     /locus_tag="ACGE4U_002621"
     CDS             1305..2459
                     /locus_tag="ACGE4U_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFF8394711.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          854 a          443 c          521 g          656 t
ORIGIN      
        1 acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga
       61 tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga
      121 tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc
      181 tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt
      241 ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg
      301 aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga
      361 aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc
      421 attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta
      481 acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa
      541 ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt
      601 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      661 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      721 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      781 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      841 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      901 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      961 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
     1021 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
     1081 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
     1141 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
     1201 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
     1261 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
     1321 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
     1381 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
     1441 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
     1501 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1561 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1621 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1681 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1741 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1801 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1861 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1921 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1981 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     2041 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     2101 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     2161 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     2221 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     2281 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     2341 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     2401 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     2461 agatgtatat aatt
//
LOCUS       DAXWLF010000224         2464 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXWLF010000224 DAXWLF010000000
VERSION     DAXWLF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.230"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            34..516
                     /locus_tag="ACGE4U_002622"
     CDS             34..516
                     /locus_tag="ACGE4U_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFF8394712.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            553..1563
                     /locus_tag="ACGE4U_002623"
     CDS             553..1563
                     /locus_tag="ACGE4U_002623"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFF8394713.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1678..2391)
                     /locus_tag="ACGE4U_002624"
     CDS             complement(1678..2391)
                     /locus_tag="ACGE4U_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394714.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          839 a          405 c          500 g          720 t
ORIGIN      
        1 tctcaaataa taaaatagaa aagagacata aaaatgattg acttggcaaa gaaattctta
       61 cacgaaaata tatattttga gttgtatcat catcgagcca tttatacaaa tgaagatgct
      121 ttggtaataa agaaagaaca aggatttaca ggtacagaga ccaaaagctt attcttaaaa
      181 gataaaaaag ataataacta cgtatatctg acttttacta cgaaacaaac ggatttcaaa
      241 gcattaggga agatcgtcgg taaaaggctt tctattgttt catcagaacg catggaagaa
      301 caaacgggac aaaagcaggg cgctgtttct ccgtttggtt atgaaaaaca gattccgatt
      361 attattgatg aagagctctt atcaaatgaa aaacttgttt ttgcaccagg tagaccagat
      421 caaacgatgg tcatccaaac ggttgactta aacaaaatca tccagctact tgagatgccg
      481 atatttttga tgccggcaaa cgaaaaaaac aattaaccaa gtaaaatagt gcatacttgg
      541 aggtgaagca acatgataag ttatccatta ttagaaagaa atgcaacgat tggggttact
      601 gcaccatcct caggcgtatc agaggaacac cattcgttac tcgaatcagc tttaaagaga
      661 atggaagaaa aatctttcca agtagttact ggaaagacgc tttggacgca agataaggaa
      721 aaatcaggtc cagcaagaaa acgagcagat gaattcaatc aaatgatgca agatgaaaat
      781 atccaaatga tctttccgcc ttggggagga gaattattga ttgagattct cgattttgtc
      841 gattttgatg ctctacaacc aaaatggata gtaggttatt cagatattag tacgttgcta
      901 cttgctgtca cattaaaaac agggatagcc actgcacatg gaaccaacct tattgattta
      961 agaggagagt acttggatga aacgacagca atgtgggaaa gtgtactgaa aacaagagaa
     1021 ggagaatcga ttctacagca accttctgaa aagtatcaaa aagagtggcc ccatgagcat
     1081 tcatcatctt ctgttttcca tctcactgaa ccaacaaatt ggcaatcagt cagcggagat
     1141 gcagtaagga tgaagggacg attattaggt ggctgtattg atacgatcag tcatttaatc
     1201 ggtacccctt atggagatgt ccaatctttt aaaagaaagt ataatgatgg aaagccgatc
     1261 ttgtggtttc tagaaaattg tgagctatcg tcagttgatt taagaagatc acttgttcag
     1321 atgaaactgg caggatggtt tgatggcagc tcggggatca tgttcggtag aagtgcagca
     1381 gaggatttgt tagattataa aatgaaggat gtatatgaag atctgtcaaa ggaattaaaa
     1441 ctaccgatct tgtatgatat tgattgtggt catgtaccgc cacaactgac attgatcaat
     1501 ggtgcctatg cagaagtagc ggtagaagat agaaaagcga ctgttttgca aacgtttatt
     1561 taaaaaattt tttcctgcct tggagtttat caaagaccaa caaatctaaa tagaaaagaa
     1621 aatgaaagaa gctgacagta ttttgccagc ttctttttgt atgatctatt agaaatttta
     1681 attatccata actatcacat tgatcagctg tttcacatca tttggattac ctgtaggaat
     1741 agctactaca gatgcattat caataatttc ttgagttgtt tctctaatga acgctggata
     1801 agatgtttga tctgcaggca tttgccaatg tgaaggaact ggataactag tgattgtccc
     1861 gtcatggtta cttgaataca ctaaaactcc ctggtaactg tatttcccag tgagcataac
     1921 cacatctttc ggatagactg cactccctgc atcataaggg gcgataggtg ttcctgcagg
     1981 ataatgaata acatgtagtt cgaaatcacc cgatgtatct ctaaaagtat tacctctaat
     2041 tgctagccaa acacgtgcgt actcaatttg tagatcagaa tatcctgcta acggatctat
     2101 tgactgagaa gactgtgagg aagttgtgtc tattgttgaa gaagaaatat ttgtctcaac
     2161 tgtactacta gatgtagaag aagtattggg ctctgagact ttactgcttg ttgaagatgt
     2221 attcgtttca gtagaagctg atttctgagt acttgatttt tcagctgaac tggtttgttt
     2281 gattgaactt ttgtctgtgg tcgcagatgg atcactattt tctttactag gagacgtaca
     2341 tcctacaagg aataaagaga ccaacaataa aataaaacct aactttttca tataaaatac
     2401 ctcattattt ttttaatacc agtatagcat aatcctgaat ttttgaatcc gtaaaacctt
     2461 gtct
//
LOCUS       DAXWLF010000225         2424 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXWLF010000225 DAXWLF010000000
VERSION     DAXWLF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.231"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(209..1393)
                     /locus_tag="ACGE4U_002625"
     CDS             complement(209..1393)
                     /locus_tag="ACGE4U_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFF8394715.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1418..>2424)
                     /locus_tag="ACGE4U_002626"
     CDS             complement(1418..>2424)
                     /locus_tag="ACGE4U_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8394716.1"
                     /translation="LPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQV
                     RVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTPD
                     SIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDDT
                     LLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTSTE
                     EGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQLP
                     DELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSAL
                     "
BASE COUNT          692 a          534 c          388 g          810 t
ORIGIN      
        1 tattgggatt ttcctgaaaa tcaataaata aaatgccagt cttttccctt ttgaccactt
       61 ttccaagcaa aattccctaa gaagatcctg taaaaaagaa gggaaattga agttaaatta
      121 ccattttgcg gtcttctccg caaaaaaaac acatgcaaga attccctctt gcatgcgttt
      181 catcttttat cctaacgaat gctttggctt agccgcgcag acgctccaca tcacgggcaa
      241 tcattagttc ctcatctgtt gggatcacta atactcttac ttttgattct tttgtagaga
      301 tatctacttc ttcaccacgc aattcattca acgctggatc gatttcacaa ccaaaccaag
      361 acataccttt gatgatttca ctacgtacat gtccgtcatt ctcgccgata ccagcagtaa
      421 agacaattgc atcaacacca ttcatcgttg tcacataacc gccgatgtat tttcggatgc
      481 ggtccacgaa aatgtcataa gctaaacgaa tatgttcttt ttcatagttc ttttcaagat
      541 cacgcatatc actagacaat cctgttaggc ctagcagacc agatttcttg ttcaaaacat
      601 tgatcatttc atcaatatca atctctaatt tttccattaa atatgggaga acagctggat
      661 cgatatcacc tgaacgtgta cccattgtca ccccagctaa tggtgtgaat cccatagaag
      721 tatccatgga tttacctcca tcgactgctg tgatcgatac accatttcct aagtggcaag
      781 tgatgatttt tagttcttcg atcggtttgt tcaataattt tgccgcacgt tccgacacat
      841 aacgatgact tgtgccatgg aagccatatt ttcttacttt gaaatcttca taataagaca
      901 ccggtaagct atataaataa ttttgctttg gcattgttga gtggaaagat gtatcgaata
      961 cagccacact caaaatgtca ggtaaaatct ttttaaacgc tttgatcccc attaagtttg
     1021 caggattatg caaaggagca aattctgcta attcagcaat gcggtccatt actttctcat
     1081 cgatcactac tgaatcccca taagtttctc ctccgtgaac aacacggtgc cctactccgg
     1141 tgatttcatc ataagagctt aaaatttgca gttcaatcaa ctgatctaac agcattttta
     1201 ctgctacttc gtggttttcg atatccatga tttgttcgaa tttttgatta tcgccgtact
     1261 tgatggtaaa aatagaatct tgtaaaccga tacgttcgac gatccctttc gcaactacgg
     1321 tttcttctgg catttgatat aattgccatt tcaaacttga actcccagca ttgattgcaa
     1381 ttgttttaga catatgactc tccttctcgt tgaatgatta cagagcagat gatttccagt
     1441 ttttaaactc gttgaaaaat tccgtcacct tctctggttc ttttaatgaa gcaagtttca
     1501 caagcaagac ttcttttact tgttgtgctt gtggtccttt tttctgtaag atcaaaatac
     1561 ttttttgcga ttgtttatta cggaacaatt catctggcaa ttgaatcatt ccttgaagat
     1621 aaccttcttc tttaaaccat tttttgattt cttcactttg atccgtttct aaaaatccac
     1681 tcggcatcaa aaacaaacca aaaccatccg gcttcacata tttcattgat tgctctagca
     1741 ataaatgatg tgcataacta tggccttctt cggtacttgt taaaaactct tttgcttttt
     1801 cgtcattcgg ataataaccg attggcaaat cgctgattgc aaaatcgaca ggatctatta
     1861 ataattcctg caacccgtct tgatgaaagt attgaacatt tgcttgtgtc aaatcacttg
     1921 tagaagctgc gacagccaac aatgtatcat caatatccac accaattcct tctgtttggt
     1981 accccgcatt cgataggttt aaaagaactg ttaataacaa gttgcccatt cctgccgcaa
     2041 tatccagaat cttaaccggt gcttttttat ttgtgtatag ttgttcaata agaaaaacaa
     2101 ataaaaaacc gatactatca ggagtgagtt gatggttcgc ctgcaaatgt tctgtctggc
     2161 tgcccttcaa taaaagtaat tgagacagtc tgcgccattc ttctggctcg aaagaaagtt
     2221 tcttcaactc ttcataaaga gctgtgatcc gttgcgttgt ctcttttgtc ggcactccat
     2281 ccaccacacg tacttgataa tcatctatta aattttctgc attttcgaca taggcttcta
     2341 aaaaggaagt tcccaatgca ttctgtaaaa gctgaatagc ttccaggttt tgattaaacc
     2401 cttgttccat tttttctggt aaca
//
LOCUS       DAXWLF010000226         2423 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXWLF010000226 DAXWLF010000000
VERSION     DAXWLF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.232"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..1446)
                     /locus_tag="ACGE4U_002627"
     CDS             complement(<1..1446)
                     /locus_tag="ACGE4U_002627"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8394717.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEEE"
     gene            complement(1784..>2423)
                     /locus_tag="ACGE4U_002628"
     CDS             complement(1784..>2423)
                     /locus_tag="ACGE4U_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalt transporter"
                     /protein_id="HFF8394718.1"
                     /translation="KKDKLYWEEIKASIRMNATNLDGDREIRYSINLQKHLLEIKKLA
                     DKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQ
                     LLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIK
                     DPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIYITEFDDFNDYPF"
BASE COUNT          700 a          451 c          405 g          867 t
ORIGIN      
        1 ttcttcttct tcaaaggtat ccacgtaacg gggaatgttt aagttgtaat cattctccac
       61 aatttcatca aattctgcga cataagcata cttgtcttta attgtacggg ttttataggc
      121 ctctaaaata gcgtcaatat ggtaatctcg tagcgtattt tgcgttttta ttttttcaaa
      181 atcttgtgac gcatcaataa agagcacatc ttttttcgca cggtctttct tcaagataat
      241 tacggtcgtt ggaatggacg tattaaagaa gatgttagca ggtaagccaa tgacggtgtc
      301 aatggcacca ttttctaaca aggctttgcg tatcttccct tctgcgcccc ctctaaagag
      361 caccccatgt ggcaaaacaa tggccatgac gcccgtatct ttcaaatgat agtagccatg
      421 taataagaaa gcaaagtccg cttttgattt aggggctaat acgccatagg cagagaagcg
      481 tgggtcatct aagaagcctt tatcagccga ccattttgca gaatacggtg ggttcatgag
      541 caccccatcg aagttcgttg gctcttctgt tggccagtct tgatccaagg tatccgcatt
      601 atgcaagtgt tggttggcaa tgggaacccc atgcaaaatc atgttcatac gcgccaaatt
      661 ataggtagac gtattcaact cttgtccgaa ataagaaatg gttcccggtt gatgggaata
      721 ttttttagcg ttcaaaagta acgagcccga acccatggtc gcgtcgtaaa cagaaaagcc
      781 ttttttatct tcttttcctt gtaaaacaat ttgtgtcatc aattttgcga ccggttgtgg
      841 ggtatagaat tcaccggctt ttttaccgga ttcagatgcg aattggccaa ttaagtactc
      901 ataggcatcg cctaagacat cgccagcatg ccccgccatg tttaaccctt ctaattcttt
      961 cataacatct gcgatggttt tattttgttt ttgtggggtt gaccccagtt ttttagaata
     1021 cagatcaatg tcttcaaata agttctcaaa gttttcgctg gattgttcaa tgtcacggaa
     1081 accttgtacc aggtcttcta attgaaaaga tccatcatga atggctgcca cttgcgcggt
     1141 aaaggtcaat gcgggtgcga tggtatatga aaactcatct tttagttcat acaagagatc
     1201 atcctttata tctgcatctt taaatgcttc aacgtaaagg tcttgtgctt tatttaggtc
     1261 gtccgtttct tcctccaata gaacggagac ataccgcagc atgttgtcgg ataagtattt
     1321 ataaaagacc aagcctagta aatagctctt gtattcatta gcgtccattt ttgaacgcag
     1381 gatatctgcg gaattccaca gtgcctgata taagcttttt gaattttttt cgatttgttc
     1441 tgtcattggt tattttcctc attcgtgttg tattttttat aaatcagttt tttaaaaaat
     1501 tagttactat ttgaacttgt tttttcacca tatgtatttt aatgaaatgg ataagctaca
     1561 tttgcttaga caattcatgt cttacttaat tcttattata acaaacctat ttcacctttt
     1621 tcgatacatt aaataataag taacttaaaa taaggttcga ataaccttgt taaatagtgg
     1681 ttttcttatc ttatagtata gaaatcaaga gattacttcc gatataacat ttttttaaaa
     1741 aaattagatt gtaatgtttt ttatattcat tctaaatttt cacttaaaaa ggataatcat
     1801 taaaatcatc aaattcagta atatatattt tcttactcat tttaatatat ttctcttcgt
     1861 ctttaatggt cccttcataa aaagatttct tagcatcttc atttagccag tctacagaaa
     1921 acatctgatt agagacagga tctttgattt tagatacaaa ttcggatgat aaaacactat
     1981 caaacatttc tccaaaaatg gtggtcaatc tttgaaacca tatcatcata aattcagtat
     2041 ttggctttaa agcatatttg tttataatca tttcaactat ttctctagcc ttactctcat
     2101 tttcgaaatt taataactgc cctataatta agacacactg cggcaaagaa cccgggttat
     2161 ttatcatgat atctaccagg ataccaataa gtggtaaaag atcgttcggc tttgaagtga
     2221 ttttaataat gcgttcttta ttaaactcag aaagtgcttt tttcaattgg cctgaattag
     2281 gatgcttatc tgctaacttt tttatttcca ataaatgttt ttgtaaattt atactataac
     2341 gaatttctct atcaccatct aagtttgtgg cattcattct tatacttgcc ttaatttctt
     2401 cccaatagag cttatctttt ttg
//
LOCUS       DAXWLF010000227         2405 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXWLF010000227 DAXWLF010000000
VERSION     DAXWLF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.233"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            28..240
                     /locus_tag="ACGE4U_002629"
     CDS             28..240
                     /locus_tag="ACGE4U_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394719.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(518..2317)
                     /locus_tag="ACGE4U_002630"
     CDS             complement(518..2317)
                     /locus_tag="ACGE4U_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFF8394720.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          841 a          440 c          450 g          674 t
ORIGIN      
        1 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
       61 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
      121 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
      181 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
      241 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
      301 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
      361 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
      421 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
      481 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
      541 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
      601 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
      661 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
      721 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
      781 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
      841 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
      901 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
      961 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
     1021 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
     1081 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
     1141 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
     1201 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
     1261 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
     1321 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
     1381 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
     1441 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
     1501 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
     1561 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
     1621 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
     1681 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
     1741 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
     1801 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
     1861 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
     1921 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
     1981 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
     2041 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
     2101 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
     2161 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
     2221 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
     2281 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
     2341 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
     2401 ttaac
//
LOCUS       DAXWLF010000228         2281 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXWLF010000228 DAXWLF010000000
VERSION     DAXWLF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.234"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..1299)
                     /gene="ltrA"
                     /locus_tag="ACGE4U_002631"
     CDS             complement(<1..1299)
                     /gene="ltrA"
                     /locus_tag="ACGE4U_002631"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFF8394721.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHI"
     gene            complement(1868..2245)
                     /locus_tag="ACGE4U_002632"
     CDS             complement(1868..2245)
                     /locus_tag="ACGE4U_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394722.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          552 a          513 c          413 g          803 t
ORIGIN      
        1 aatatgactt ttggagactg accaaatacc ttttcttgta tttgaccatt catacgcttt
       61 cgctttatcg atccctaaac gaatcagatt ttttcttttg gttcttggat tcttccattg
      121 tttccaaata tattgtctga ttcttcgctt caaccaaccg tttaactccc tcatgaattc
      181 tttcatctca ccaattccat agtagttgat ccaaccagtc attagttgct tgatttcttt
      241 taaaataagt tggatacttc ttccacggtt tcttttagtg agttgtttga gcttgctttt
      301 cacacgctgt tttgcttttg cgtgagggcg aattttaata cctttctttg ttggcagaat
      361 gcagaatcca agaaactttc gtttcaatgg acttcctacc gcactctttt ctcgatttac
      421 cgtcaacttt agttgttctt cgagaaattg aatggatttc ttcatcacac gctgtcctgc
      481 tcgtttgctt ctaacataga tattgcagtc atcggcgtat cgaacaaatt tatgtcctag
      541 attttctagc acttgatcaa attcgttcag gtagatgtta cttaacaggg gagagagatt
      601 gcctccttgg ggcgttcctt cttcagttgt tgtgatgagt ccattttcca ttacaccact
      661 cattaagtat tgacggatca gttttaggac ttgtttgtcc tttactttcc gctcaatgaa
      721 atacatcagc ttatcgtgat ttacggtgtc aaagtaggct ttcatatcaa tgtccaccac
      781 atatcggtaa ccttgttcat aataagattt tgctttcttc actgcgtcat gtgcgctgcg
      841 atttgggcga aacccataac tattatctga gaattctggt tcaaatagcg gttgtaacac
      901 ttgattgatt gcctgttgaa gcatacgatc aataaccgtg ggaattccta gttttcttac
      961 accgccgttt ggtttcggta tttctactcg caaaacaggc tgaggtttat agttcccttg
     1021 gcgtaaattg gatagtaatt cctcgcggtg ctctttcaag taaggaagca gttgatcgac
     1081 ggtcatccca tctgcaccag ccgccccttt atttctgact acttgaagat acgcttgatt
     1141 gagattattc cgatctagaa ttctcccaaa cagattttct acaccatctt gtttcctctg
     1201 ttcaccgcaa gaagtgctgc gcacttcatc atacccttcg tgttccacac tagctctttg
     1261 atgatagcca agttttcttg ttttctgcgg tcgtcgcatc ttctcacacc tcctaaatta
     1321 ttcaagattc ttattgttca gtccttcgta acggtcagtg tcgttactac tatgacctcg
     1381 gctgacttct catgattcac catcatgtcg ccatgatcgt tgtgctggtt tgttttcatt
     1441 caacgcccac aacaccctca tgagatctcc cccggtaaga gtgaaaactt tcctcccatg
     1501 taactgctag atttactgta taggtttcgg gcagtattgg actttgtctt gtgatgaaga
     1561 cttatccgac ctaattcagc cttgtatcta gtttctgttc gtcagttcag gagtttgcgt
     1621 ccgacttcct tcagccacaa cctcacgatt gccgccttgt ctttcgctaa cagttcctac
     1681 tgccaagcct gtagtggact ttcaccacca agttttcacc catgcagggc gcacaccaaa
     1741 aaagaagcag atccgttagc ggttctgctt cttttagttt attatatttt cttatcaata
     1801 ataggaacct gattttaaaa gcggctacct ctttaccgtg cctctttaca tacaaaaaat
     1861 agaggaatta agcgtaaatg gcttggctat tataactaaa atgctggtta tggagatgtt
     1921 gcagcaagtt ttcgagaaaa gcgtattcca tctcttgtcc attgaataag atctggatac
     1981 gataatgatt tttgaaacgc ttctgttcat gaatctgact gattgaaagt tcgcctttgt
     2041 gttctgtctg ataaagaaaa gtccttttac ggcattcagc acagcgcttg gttgctcggc
     2101 ggatttttcg gaatgtataa aaactagtat ctttattgca taaataaacg tctttgatcg
     2161 acagcaaatc attatataga caggaacgtt cttcagctgt taattctgat aaaaaaagct
     2221 gaccttgttt tattgcatct tccatcggca agtgcctcct cgaacccatt atccaaattt
     2281 t
//
LOCUS       DAXWLF010000229         2271 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXWLF010000229 DAXWLF010000000
VERSION     DAXWLF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.235"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            192..446
                     /locus_tag="ACGE4U_002633"
     CDS             192..446
                     /locus_tag="ACGE4U_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8394723.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="ACGE4U_002634"
     CDS             447..764
                     /locus_tag="ACGE4U_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8394724.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1323..2258
                     /locus_tag="ACGE4U_002635"
     CDS             1323..2258
                     /locus_tag="ACGE4U_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394725.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          430 g          711 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaaggca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacatccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattacta
     1381 ctttcaaaag attatccgag ttaatgcagc agactgttaa aagtgctatg cctgaaggat
     1441 acaagtttaa ttttgttcaa tcttcagtat ctactgaaag tatctatttt tcaattcttt
     1501 ctgagagtag gctttttcat tttagaataa gttctcatgt aaagaaaact gatgttgata
     1561 tatatacgtt ccgtacaaat aattataaaa atttaaatga tttgagtaga agtatcagtg
     1621 aatatctcaa ggggaatagc tatctcaaga ttcgattttc acatatatta attttactac
     1681 catatttgaa tgagaatttc accctaattc aagtagataa gtctttcatt gcctcaggta
     1741 atcaaggaaa tatgaatatt acaagtaaca aaacgtcacg tgtgctattg gcgttgatgg
     1801 gttacggaat tctttataga gatgaccaaa atggccagat tctaataaat cgggctgggc
     1861 gttcattatt aatgaaatat agaaaattta taccggaaga ttttcaaaac ttacagttag
     1921 aaaacatgcg tttagaagat ttatttagat tagcagaaga tgataagtat agatttgaag
     1981 ttttgaaaat tcaaaagaat gaactattga ataagttata tcctgagaat gcgataaaag
     2041 cacattttgg tgatgctaat catatgtata gttggttcat tcctaaaaga tttagaggga
     2101 aaatcaatgc tggagatcaa gtgaaagtac tcaatcgaaa tagtacagat gtcgtaatga
     2161 taacagatat ctatgtagaa gatgaagata taatcaaata tatgaagcct gtgataaaac
     2221 ttgttaataa aaataaagga acccgtggtg ctagttaatt ttttcctaga g
//
LOCUS       DAXWLF010000230         2262 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXWLF010000230 DAXWLF010000000
VERSION     DAXWLF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.236"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            220..432
                     /locus_tag="ACGE4U_002636"
     CDS             220..432
                     /locus_tag="ACGE4U_002636"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFF8394726.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            434..2113
                     /locus_tag="ACGE4U_002637"
     CDS             434..2113
                     /locus_tag="ACGE4U_002637"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFF8394727.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT          727 a          439 c          469 g          627 t
ORIGIN      
        1 gctagaataa caattaattg ttgtatattt tttatacaga aggtacggtt gaaaaaatat
       61 ccgaacagat aagcaaaaac gaggcttgtc tctttgactc gaatccagta ttctagtcaa
      121 agcatatgaa aaaccaggtg gcttgtcccc tcgttttgtg ttgtctaaaa aaatcaacta
      181 gctaaatcac tgctttacgc agataaagga gacaaaacaa tgatctataa agtttattat
      241 caagaaacaa aagtacgcaa ccctaaacgg gaagaaacaa aatctcttta cattgaggca
      301 aaaagcgatg tagacgctcg ccaacgtgtg gaagaaaata ccccatacaa tattgaattc
      361 gttcaacttc tagaaggaag tcatctagag tacgaaaaag aaaatgctga tttctcatta
      421 gtggagtttt aatatgaaac tttccatcaa aaataacgag acaggcgtct ttgctattgg
      481 tggattaggt gaaatcggga aaaatacgta tggggtacaa ttccaagatg aaatcatttt
      541 gattgatgcc gggatcaaat tcccggaaga cgatttactt ggaattgact atgtcattcc
      601 cgactacagc tacatcatac aaaacttaca caaagtaaaa gccttggtga ttactcacgg
      661 gcacgaagac cacatcggcg gaatacccta cttgctgaaa caagcaaata ttcctatcta
      721 tgctggtcct ctcgcattag cgttgatcac aaacaaatta gatgagcatg gcttgcttcg
      781 agatgcagag ctccatgaga tcaatgaaga tacgatcatc cgttttagaa agacttctgt
      841 cagctttttc agaacgaccc atagtatccc agatgcatta gggatcgtgg tgaaaacacc
      901 ttctggaaat atcgtagcga ctggagactt caaattcgat ttcactcctg ttggcgaacc
      961 agcaaacttg cataaaatgg ctcgtatcgg ggaagaagga gtcttgtgcc ttttatctga
     1021 tagtacaaat gctgagattc caacatttac taaatcagaa aaaacgattg ggcattctat
     1081 tttgaagatc ttttcaaaaa tcgaaggacg tatcattttt gccagctttg cttctaacat
     1141 cttccgtttg cagcaagcag cagatgccgc tgtggcaact ggtcgtaaaa tcgctgtttt
     1201 cggacgaagc atggaaaatg ccatcgtcaa cggtcaacgt ttaggatata tcaaagttcc
     1261 agacggcaca tttgtagatg cacacgaaat caatcgcttg cctgccaatg aaacgatgat
     1321 cctatgtaca ggttctcaag gagaagcgat ggccgcgttg agccgcatcg cgaatggtac
     1381 tcaccgtcaa atccaaatcc agccagacga tacagtgatc ttttcaagtt cacctattcc
     1441 tggtaataca acaagtgtca atcatttgat caatttgctg tctgaagctg gtgcagaagt
     1501 gattcacgga aaaatcaata acatccatac ttccggtcac ggtggacaag aagaacaaaa
     1561 attaatgctt cgtttgatga agccaaaata ttttattcct gtccacggag aattccgcat
     1621 gttgaaagta catgcttctc tagcacaaga tgtaggtgtt cctgcagaaa actgcttcat
     1681 catggaaaat ggcgatgttt tagcgttaac tagcgattct gcgcgtcctg ctgggcattt
     1741 tgtggctgga gatgtgtata tcgatggtag cgggatcggt gatattggta atgtcgtttt
     1801 acgtgaccgt cacctgcttt cagaagaagg tcttgtatta gctgtcgcga cagtggatat
     1861 caagaaaaaa gaaatcttgg ctggtccaga tattctctct cgtggatttg tctacatgcg
     1921 agaatcagga gaaatgatca atgaagctca gcgaatctta ttccacgctt taagagaatg
     1981 tttgaatgga cctgactgtt cagaatataa attgaaagaa gcgatgactg gcgcgcttca
     2041 gcctttctta ttcgaacgta cagaaagaca tccaatgatt ttgccaatgg taatgacacc
     2101 agatgaagaa taaataagag gttatgaaat cagcaccttg tcctgaatat aagagaaaac
     2161 aaatgaaaaa catgagaagc catttttgtt tgttcttatt ccttgataca aggtggctct
     2221 ttcatagcct cttaaattta tttacttttt catacaaaaa aa
//
LOCUS       DAXWLF010000231         2227 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXWLF010000231 DAXWLF010000000
VERSION     DAXWLF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.237"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..1405
                     /locus_tag="ACGE4U_002638"
     CDS             <1..1405
                     /locus_tag="ACGE4U_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFF8394728.1"
                     /translation="NILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLNDINNIKKE
                     FEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQLTE
                     CEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWEL
                     PESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIK
                     KRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFI
                     DNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQT
                     NLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHY
                     LIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIP
                     ISINTIDRAIISAVQNK"
     gene            complement(1497..1862)
                     /locus_tag="ACGE4U_002639"
     CDS             complement(1497..1862)
                     /locus_tag="ACGE4U_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFF8394729.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1859..2224)
                     /locus_tag="ACGE4U_002640"
     CDS             complement(1859..2224)
                     /locus_tag="ACGE4U_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFF8394730.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
BASE COUNT          737 a          425 c          367 g          698 t
ORIGIN      
        1 aaatatttta agacagctgt ttttatgtgg gcaattctat gtaaataaag aagtcaatct
       61 agaaaagctc tccaatctcc tgcatgtttg caaaaccact ttactcaatg atatcaataa
      121 cataaaaaaa gaatttgaag aacagatcgc ttatacacat cgggaaaagg accgttacac
      181 tttatatttt tcagaacata tacctcgatg caagatcatg cagcaattgt ctcaaaattc
      241 gttgttttta aaaacttgtc tgctttattt ggaagaagat gaacctgatt atcttcaatt
      301 aactgaatgt gaattcatct ctgtcagcaa agcttattca ttaaaaaaac aagtattagc
      361 ttattttaat gactgcggga tcgaaatcga ccgctactcc cctcgattca ctgaaatgga
      421 aagacgtctg ctgcttctta atgtgtcgta tcgtttaggc ggttttaatt cttgggagct
      481 acctgaatcg ttttttgaac gtgccgatcg tttcattgaa tccgtaacgg aaaatagtgg
      541 cagattttat gataaagaaa ataaagaaat cttatcgatt ggttttgcta tcagtttcct
      601 acgccagcaa gtgtgtgctg tgacaataga ttcaaaattt atcgaagaaa tcaaaaaacg
      661 tcctatttac aattatgtcg aatcagcatg ggaaaacact gatttccaaa cctattataa
      721 aaaagaagaa ttcgccttta ttttgacact attcaatctt tgtaattatg gtttccattc
      781 ttatcagttg attgcggaag actttcaaca gctacaccaa gtgtttattg acaatactcc
      841 cgaaataaaa gaactagttg ctacctttga atctcacttc aatcaagaat tattcggaaa
      901 ccaaccgttc gaacgtgcgc tgattcatct gatgcgttcg gcttgggata actatcaatt
      961 attcatgcca gaaaaatttt atcttttaaa tgaggaacag acaaatttat ataaagaagt
     1021 ccagacaatt ttttccagct ggtccagcca gttaccttat gatctacggt tgaatcctaa
     1081 ttgcatgcgg gcttttgtga tcgaattgag cggaattctc cgtctgacaa aagaacatct
     1141 gactatctat atcgtcacaa attcggatgt tcattatctg atttatcggg aagccttgga
     1201 agctgtaact actttcgatt ttcaagttgc accaacaatt tattcaagta tatctgatat
     1261 aaagaagtat gcgcagcagt cctcaaaccg ggtattatgt gaacgtacat tatatacacc
     1321 agatgcggtc caatatgaaa atattattcc tatttcgatc aatacgatcg atcgagctat
     1381 catttcagct gtgcaaaaca aataaggggc tgtgacaaaa atcatgtcac agcctcttat
     1441 ttgttgaaga tgcccatttc tgtaacaatc tcttgataca cgtcaggctc acaacctcac
     1501 ttattttgga catctacggg tccgatctct ttatttaaaa aataagaaat gatggttcgg
     1561 ataactacca acgccgctag ttggaaaata tcctgcaagg taggtttgat aatcgattcg
     1621 atgatcccag ctgaaacaag aatttccaaa cttagaagta tatagcctcc caacatcttt
     1681 ttaatagcat tgttctcgct gactcggtcg ctttgcactt ttgtatggac aaataaatat
     1741 ttcaaaaaat ccttgattga tagacaaaca ccccaaacca ataaacagat agcaaagata
     1801 tcaagtccca gtacaagttt ttcgaaatat ggaagaagaa actgcatcaa ctctgtcgtc
     1861 atcctacctc tcctcttctt tgtttttctt tgtcttctat ttcctgattc aagaaataag
     1921 aaatgactgt tcgaatgata ataataagtg caagcttcaa aatatctgtc caagtcggct
     1981 tgataactga ctcgatgata tcagcaacga ctagtacttc caagcttaac aaaatataac
     2041 ttgctagcag ttttcttgta tctgcattac gagtattacg ttctgcatag tttttcttga
     2101 catgagacca atcaaaaagt ttgaatagaa caagaacgat cccgacaatt aagataccga
     2161 tagaacaaat atttaaaata gcaatgatcc attctagaaa ttcaattaaa tttgagcgaa
     2221 acatata
//
LOCUS       DAXWLF010000232         2163 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXWLF010000232 DAXWLF010000000
VERSION     DAXWLF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.238"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..567
                     /locus_tag="ACGE4U_002641"
     CDS             <1..567
                     /locus_tag="ACGE4U_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394731.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
     gene            complement(614..850)
                     /locus_tag="ACGE4U_002642"
     CDS             complement(614..850)
                     /locus_tag="ACGE4U_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394732.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            948..1127
                     /locus_tag="ACGE4U_002643"
     CDS             948..1127
                     /locus_tag="ACGE4U_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394733.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1258..1527
                     /locus_tag="ACGE4U_002644"
     CDS             1258..1527
                     /locus_tag="ACGE4U_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8394734.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1520..1777
                     /locus_tag="ACGE4U_002645"
     CDS             1520..1777
                     /locus_tag="ACGE4U_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8394735.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          795 a          303 c          431 g          634 t
ORIGIN      
        1 gaagaacaat tgtatttgga aagtgaaacg agtcaaacga aggtcacagt cagccataat
       61 cgtttagacg tgtataaacg tcgtcctgaa acggtaaaaa aaggaacgga gcttctacga
      121 ctagaaacag aaaaatacat catgaatgtt tacctagaaa ggagtgagct ggtcgaaaac
      181 tttgtcagct acacgatttg caggaaacac tattactcag aatcagaata ttattcaatt
      241 tttgagagca tggaattaat ggaagaagaa atggaagagc taaacagcaa agatgcgacg
      301 atagacgatt attaccttgt atttactaga tttttgacgt gtggaacgga atctatcgtg
      361 ttaatgggaa tggattatga actcgctgta cccgaagcat ttttgaggga atatgtagaa
      421 gaagtagata acccaggcga cgactggtac acgactttaa ctgatttttt agacacctat
      481 acttacgacg atacagaaga aatccaaaat gaacttgacc gtaaacgaat gccatatcaa
      541 cgatttgaaa ggaaaaaaag gcaataatca tttataccga aaagtgagaa tgtgtgaaat
      601 aaggacaaaa aacctagtaa ctaagggaat ggttcttatt tcgcaccaat cgctccaacc
      661 gcccccgccg cttaggtggt gcgaaataag cgggcaacac ccgataacct aagagaaaga
      721 aggagatcca tatgaataaa atcattcctc tattgttagt cctatttgtt ggcgaaatac
      781 ttatgcgcca tgctgcagct gtgcatttaa acttcgtagt atttttggca atactggtat
      841 tttgtttgat cttttggcgc tttgtattag cccttgtgag tctactattc aagatgattg
      901 cagccatatt tttcataggg ttatttcgat gaaaggagag agtactaatg ccaattgcaa
      961 taggaaataa acggttaccc gttacgttgg atgaaaagag acaaaaagaa ttgcagcaac
     1021 taaagcagaa gtacggcaaa agtgaatcca ggattatgtg tattgcgtta gatttattga
     1081 ttgcccaaga aaaagcagga tttgaggtac cagcactcaa aaagtgacgt cactttttat
     1141 cctaaaaact aaaagtgata gcacttttaa ttataagaag ttagaatatt aatcatttgc
     1201 ttaattgtac aatataatgt acaattgttt tatagaaata aataaggggt gaaaggaatg
     1261 gaagcagtag cttattcaaa tttccgccaa aatttacgta gttatatgaa acaagttaat
     1321 gaggatgctg aaacacttat tgtaacaagt aaagatgtag aagatacagt tgttgtatta
     1381 tcaaaaagag attatgattc tatgcaagaa acgttgagaa cactttctaa taattacgtc
     1441 atggaaaaaa ttcgtcgagg agatgaacaa ttctccaaag gtgcatttaa aacacatgac
     1501 ttaatcgagg ttgaatctga tgattaaggc ttggtctgat gatgcttggg atgattatct
     1561 ttattggcat gagcaaggaa acaaaagcaa tataaaaaag attaacaagt taataaaaga
     1621 tatcgatcgt tccccctttg ctggattagg aaaacctgag ccattaaagc atgatttatc
     1681 tggaaaatgg tccagaagaa ttacagatga acatagactg atatatagag ttgaaaatga
     1741 aacgatattt atttattctg caaaagatca ctattaacca atcggaagta aggaaagggt
     1801 cagaaactta aaagtttttg atccttattt tatttaccct agtcatttaa aaagctaata
     1861 tagcttagtg ttgattgtta ttaatgaatg tgtttgttac gcgtattacg gatataaggt
     1921 tagtaaaatc atttctaaag ttgaggaaaa gtaaatataa atggcttaaa tttcaacaat
     1981 ttgaagttga atagatatgt tataatacta ttgtagtgtg ggatgttagt tactaaagga
     2041 tgacgcttat atatatgact gaatagaata agcaataggt ttaataatct attttaaatt
     2101 ttttgtacta gttttagtta attagcaaaa acaacaaaaa taaacttctc atagaattta
     2161 gct
//
LOCUS       DAXWLF010000233         2110 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXWLF010000233 DAXWLF010000000
VERSION     DAXWLF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.239"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            121..2109
                     /gene="treP"
                     /locus_tag="ACGE4U_002646"
     CDS             121..2109
                     /gene="treP"
                     /locus_tag="ACGE4U_002646"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFF8394736.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
BASE COUNT          613 a          409 c          461 g          627 t
ORIGIN      
        1 aaaacgatat cattttctct cgtacttttt aataaaacgc ttgcaactct ctccgaaaaa
       61 aagtatagta aatgaagaaa caacttgcat agacaagtta agagaaaggg gtttattgat
      121 atggcaaaat atcaagcaga tgccgagaag ctgttacatg atatcggcgg aaaagaaaat
      181 attgctgcag ttagtcattg tgcgactcgt atgcgttttg tcctgaatga tccaggaaaa
      241 gcggacgaaa aagcgatcga agatatacct agcgtaaaag ggatgttcac gaatgcagga
      301 cagttccaag tcatcatagg aaacgatgtt tctacttttt ataacgactt cgttgcagta
      361 tctggagttg aaggggtttc caaagaacaa ggaaaagcag ctgcaaaaca aaaccttcat
      421 cccgttcaac gtgctattgc cgttttagca gaaatcttta caccactgat tcctgcaatc
      481 attgtcggtg gattgatcct tggattcaga aatgtacttg aaggtatcca gtttgaaagt
      541 cttggtggaa caatcgttga acactcgaag ttttggaatg gtgtcaatgc ctttttgtgg
      601 ttaccgggtg aagcaatttt ccattttctt ccagtaggta tcacttggag tattgcaaaa
      661 aagatgggta ctacacaaat attaggaatc gttttaggaa ttacgttagt ttctccgcaa
      721 ttattaaatg cttatagtgt agcttccaca gccgcagcag atattccatt ttgggatttt
      781 ggttttgctc aagtccagat gattggttat caagcgcaag ttattccagc catgctggca
      841 gggtttatgt tagtgtatct agaaatcttc ttccgcaaat atatccctca gtcgatctcg
      901 atgatttttg ttccattgtt ttcattgtta ccaactgttt tagctgcaca cgttatttta
      961 ggtcctatcg gctggacagt cggaagctgg atctcaaata tcgtaaatac tggattgacg
     1021 tcttctatca gctggttgtt tagtgccgta ttcggattcc tgtatgctcc tttagtgatt
     1081 actggtctcc accatatgac caatgccatc gatatgcagc tgattgcaga tttcggatca
     1141 acgaatttat ggccgatgat tgctttatca aatatcgccc aaggctctgc agtcttagcc
     1201 atcatcttct tacatcgtgg gaataaaaaa gaagaacaaa tctcgattcc agcaatgatt
     1261 tcttgttact taggcgtaac tgaacctgct atgttcggaa tcaatttgaa atacgtctat
     1321 cctttcgtcg ctgcaatggt cggctctgga ttagctggca tgtttgctaa cttgatgggt
     1381 gttcgagcta acgcgatcgg agtaggcggt ttgccaggaa tactagctat ccaagcagaa
     1441 acatgggtac ctttcatcat cgcaatgatc attgctgtca tcattccatt tggcttgacg
     1501 atcatcttta gacgtcaggg aattttgaat aagattgatc ctgctgttcc tgaaaatgct
     1561 gctgatgtac aacttcaaac agcaaatggc gcaacagcaa cgccacagtc tttcgaacct
     1621 gtttcagcaa ctggtacggc agttgcaaca aaagaaacat tattcgcagt cgctgcaggg
     1681 accatcaagg aaataactga agtaaatgac ccagttttct cacaaaaaat gatgggtgac
     1741 ggttatgctg tcgaaccgtc aaacggcaaa gtctatgcac cagtaaacgg taaagtgacg
     1801 agtgtctttg aaacaaaaca tgccattggt attctttcta atgaggggct ggaagtactt
     1861 gttcacatgg gattagatac tgttgaacta aagggtgttc ctttcaatgt attcgtgaaa
     1921 gaaggatacc ttgtcacacc agagaccctg attgctgaaa tggatttgcc agaaatagaa
     1981 caagctggca agaaaactga cattattgtt gctttgacca ataatgaaaa agttgcagga
     2041 ctttctcttg accaatcagg acttgttcgc ccgggcgaag cagtcggtaa agctgaagta
     2101 aaatcttagt
//
LOCUS       DAXWLF010000234         2083 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXWLF010000234 DAXWLF010000000
VERSION     DAXWLF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.240"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            84..407
                     /locus_tag="ACGE4U_002647"
     CDS             84..407
                     /locus_tag="ACGE4U_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFF8394737.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            589..1959
                     /locus_tag="ACGE4U_002648"
     CDS             589..1959
                     /locus_tag="ACGE4U_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFF8394738.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
BASE COUNT          587 a          384 c          427 g          685 t
ORIGIN      
        1 cctcgttgaa gtaagtattt ctagtttatc ctaggattaa ttataaaaat aaaacattcg
       61 ttgattcgaa ggaggttaaa cagatgtttg aaagttttga tagcagccgc aaccgttacg
      121 cttcagttgg tgtcgtatca agtttgccag atgaattgat tgacagtata tggtttatta
      181 ttgacctaga tcttaaggga gtcattccat tagaaaatat attaaccttt gatttgatcg
      241 aaaatcgtgg aaaagtgacg atgcattttt ctcaagcagg aagcaatgtg gaaatggata
      301 ttgatttgcc gtttgcctat tcgaaccaat ttcctgctca agtatacgcc tatgatgacg
      361 gtacgagaga aacgatcctt ttaccaagtg agattagaca atattaaggt gatagataaa
      421 cgtggatgtc ccttatttta cagaccaggc caagttgttg attctttgac tcaattcttg
      481 gcttcttttt atggagtttc ataaattatc attgatttct aattggtatt ctcaggataa
      541 acgctatata ctactaacta aattttaaag atataaggag tttaaaagat gcgcgattta
      601 acaaaaggaa gccccgctaa actgattttg atgttcactg ttcctttact gatcggaaat
      661 gtttttcagc agttctacaa tatgatcgat atgatcatcg tgggtcagac gctaggtaaa
      721 aatgcgctgg cagcggttgg tgcaactgga agcctgacat ttttgatcat tggctttgcc
      781 caaggtttaa cagctggttt agccattatt accgcacaac gttacggtgc aaaagattat
      841 cggggtctga agaaaagttt tgctgctagt gttgtcatta gtcttgtcgt cactgtgatt
      901 ttaactgtcc taagtctcct gtttattcgt ccgatgcttc aattgatgca aactcctcct
      961 gaaatacttg atcaagcaca aacatttatt tcaattattt tattagggat ctttgcatca
     1021 gtcagtttca atttgctttc taatgtcatt cgcgcacttg gtgacagccg cacaccacta
     1081 ttctttctga tcattgcggt catcatcaac gtcgtacttg atttgatctt tattatctat
     1141 tttggcatgg gtgtggaagg tgcagctatt gctacagtaa tcgcacaagt ttcttccagc
     1201 gtactgtgtc tagtgtatat caagaaaaaa atgcctttgt tacagttacg caaaaaagat
     1261 ttcaaatttg aaaaagaaga gtttgctgta cacctaaatg ctgctttgcc aatggcgttt
     1321 caatcttcga tcatcgctgt tggtgctatc gtgttacaag cagcactaaa tagtctaggg
     1381 acagatgtcg tagctgctca agccgctgct ggtcgtatcg accaatttgc aaatcagccg
     1441 atgatgtcat ttggtattgc aatggcaact ttcactgcac aaaactatgg tgcgaaagaa
     1501 tatggccgaa tcttgcaagg ggtacgtcaa actttactga tgagtgtcgg atttagtctc
     1561 ttagcaggtg ctatcgtcat tttcttcggt catttctttg ttcaattgtt tgttagccca
     1621 tcagaaacac gtgtatttga attagcacaa acctacttca atatcaatgg gtctctttac
     1681 tggatcttag cagtcctgtt cattttacgc tacacacttc aagggcttgg acaaagcaag
     1741 atccctacga tcgccggtat gatggaattg cttatgcgtt cttttgcagc tatcgttctt
     1801 acaggaatgt ggggataccc aggagcagca gcagcttctc ctttagcttg gatcggttct
     1861 gttgccgtgt tgctttactc ttatatgcgt tcgatgaaac aattgaaaca catgggtgaa
     1921 gaggaacatt tcacattaga acatgcagaa gcaagatagc cttgattaaa agaaatcgtt
     1981 gcggctaaaa atgaaaactt ttattgaaaa tatcttcttc gtttaccgaa aacaggtttt
     2041 ttagataaca aaaaggctgt gacaaaaaat gtcacagcct ttt
//
LOCUS       DAXWLF010000235         2079 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXWLF010000235 DAXWLF010000000
VERSION     DAXWLF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.241"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..473
                     /locus_tag="ACGE4U_002649"
     CDS             <1..473
                     /locus_tag="ACGE4U_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394739.1"
                     /translation="VIILVYFQGGNLMIKKLFLLSILTLAMAGCSDSGLATELITKST
                     EATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNL
                     CCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(534..899)
                     /locus_tag="ACGE4U_002650"
     CDS             complement(534..899)
                     /locus_tag="ACGE4U_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFF8394740.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(975..1220)
                     /locus_tag="ACGE4U_002651"
     CDS             complement(975..1220)
                     /locus_tag="ACGE4U_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8394741.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1290..1544)
                     /locus_tag="ACGE4U_002652"
     CDS             complement(1290..1544)
                     /locus_tag="ACGE4U_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394742.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1708..2031)
                     /locus_tag="ACGE4U_002653"
     CDS             complement(1708..2031)
                     /locus_tag="ACGE4U_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394743.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          772 a          358 c          367 g          582 t
ORIGIN      
        1 gcgttattat tttagtctat tttcaggggg ggaaccttat gataaaaaaa ctatttttac
       61 tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca actgaactga
      121 tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat cacagtgact
      181 acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt aagcatcgat
      241 gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag atcaaaagaa
      301 gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta aaccacgtca
      361 ggagatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag tacgctttta
      421 gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct tgatctaaga
      481 tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt atattaggta
      541 agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac ttcatcgtgt
      601 tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat
      661 ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg tttttccatt
      721 tcatccatga caagctgatg atattggaat aatctataag gagaatagtg aaaaacataa
      781 tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca ttcccgatgt
      841 tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca taatcgcata
      901 gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa aaccagcacc
      961 aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat taggtaatca
     1021 acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg aatttcttct
     1081 ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga aacagctact
     1141 tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa
     1201 atagcttctt cgatactcat ttaatcacct ttcattatag tagaatagtt ataatgacta
     1261 tagaaagcta cacatgcaat caatagtact caatgttttt tatttaaacg tatcaaataa
     1321 agaatgacga tcaataaaga caaaaccagt cctgataatt cttctataaa aacactatcg
     1381 ggttcaaagt agttgtaatt aaaaactttc cacataaagt agatagccgg aagagtcaaa
     1441 atagctaaat aaaaccgttc tttgttccaa ttcataagat aaacaatact tgcgaccagt
     1501 gcgaccgcag gcacccaaaa caaaactctc accaacgtaa tcatgtaacg gactcctcaa
     1561 cgattcatat aataaaagat aacatgttat tgcatacatc acaaatgaaa tgctaggaaa
     1621 ataaaagaag aatagatcaa tgaaaaatga aaaagcagaa cagttttctg ctgataagca
     1681 ggaactgttc cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat tgatatctaa
     1741 gtaattcaat acatataaaa agaggacgat cgcattcact aaaaaggaaa gaatgatgat
     1801 cccatagcga atggaaacat atttaacaaa tacttttgtt tgaatacggg catatctttt
     1861 tcctttatag ctgagcatca tgacaagaag caaaagggta atcacacctt gaaaaatcat
     1921 tccaataaac agaattcctg aggtttgata atttaattgg agaaaataag ctgctgccgc
     1981 aactaaatcg aataaaatcg caataaatgg caaaataatc actcctaaca ttgagaataa
     2041 ataaaagaat aacatttttt ctaaaataag caaataaaa
//
LOCUS       DAXWLF010000236         2056 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXWLF010000236 DAXWLF010000000
VERSION     DAXWLF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.242"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            83..403
                     /locus_tag="ACGE4U_002654"
     CDS             83..403
                     /locus_tag="ACGE4U_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8394744.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            421..852
                     /locus_tag="ACGE4U_002655"
     CDS             421..852
                     /locus_tag="ACGE4U_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFF8394745.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            979..1563
                     /locus_tag="ACGE4U_002656"
     CDS             979..1563
                     /locus_tag="ACGE4U_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFF8394746.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            1677..>2056
                     /locus_tag="ACGE4U_002657"
     CDS             1677..>2056
                     /locus_tag="ACGE4U_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFF8394747.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTV"
BASE COUNT          802 a          299 c          369 g          586 t
ORIGIN      
        1 tattaacacc ttttgttaaa atagtcaacg aattttaaca aataaagtta aatatttttt
       61 cttgaacgaa aggaatattg atatgataac gtttgagaga ataaaagagt tagcgaaaaa
      121 acagggaaaa tcattaaata aagttgaaga agacctaggc tatggtaaaa acgtcttata
      181 tagacttaag aactcaaacc cctctacaga acgtttgcaa gaaattgctg actatttcga
      241 tgtctctgta gactatcttc tggggcgcga agaaaaagaa acccctaaac atgtggattt
      301 atcagaaaac gatactgttt tttcttttga tggaaaagaa atatctaagg agacaatgcg
      361 taaagcgatt gcaattgcta aagctttaga ggaaaatgaa tagttggagt gatgggttgt
      421 atgtatttga aattgaaaga aatgttgagc gaatacaatt tgaaagtagt ttacatggaa
      481 atgaaagagc ctggcttcta ttatccgaag cctagaatca tttttttgaa tgaaaatcta
      541 tatggcgaaa ctgcagaagc ctttcattta tctcatgaac ttgcgcattt caccgcttct
      601 cattttgaat attcagtttt gtacgatact tctacaactt ttcactcaaa atttgaaaca
      661 gaagcagata aaatagctat tctaatttta ttaaatatct atattgagaa cgaactgact
      721 gatgaatctc agtttaatct cgaaaaattt atggagtatt attctattca gaataagctc
      781 agatacactt gttatgctgt ctgccaatgc tattttaaga aaaaatattc ttacgcaagg
      841 caatatgtat gaatacatca agaattatta aaaaaaccga tttcagcaaa acattaaaaa
      901 agcccgtgct ggcacacgga ctcatacctc atttctgaga tcgcaaatat attataacaa
      961 gaagtgagga atatttaaat ggcaaaaaaa gttatgggtc aagacggaaa aatgtataaa
     1021 gttagtaaac ctttttacaa aaaagtatgg ttttgggtat tagcagtaat tttaattata
     1081 attattggtt ctgctttaaa tggtggatca gatagtaata aagcaagtga taatggtggc
     1141 gaaaaagtaa ctaaatcttc aacctctgct tcatcttcta aagaagaaaa aagcgatacc
     1201 ttctataaga ttggtgacac tgtaaaagtt ggtgatgctg aatatacact aaatagtgta
     1261 gaactaactg atgaaagaaa tcaatttgaa gaaaaccaac ctgcacaagt agtaaaaatt
     1321 acttataccg taaaaaatga tggcgattca gatatccctg taggtacaga tgtggaagta
     1381 tatggaccag atgacaaaaa atcagagaca tatgccaacg aaaatacaat gggatctgtt
     1441 gctccaggaa aacaaatgga tgtaactgct catttcacgt taaaccaaaa gggagaaata
     1501 gaaatccact tctctccttt agtatcattt gaaaaagcag ctatttttaa agcaactgta
     1561 taataaataa aaaacacgcc caccgtccaa agcaagcgtg ttctaagaaa aacaaaccta
     1621 tacagtaggc ttctttatag ttcctattgt atcagagaaa gagagctgat tcaattatgg
     1681 caagattagt caaacgtgga aatagttggc aatacgaaat ttcatacaaa aaagatgacg
     1741 gaaaatacac gaagataaga aaatcaggat ttaagacaaa aggagaagca aaagatgccg
     1801 ccaacgaatt agaatataac ttgaacaaag gccttaaagg ggatcgcaaa aatctattat
     1861 tatcagatta ctttgaggat tggatgcaac tttataaaga aggaacagta tctcctatca
     1921 cttatagaaa atacgaagat acgttaatga acataaagaa atatatgcca gcggtattga
     1981 tttctgattt agatagagtg ggatatcaac gctttttaaa taagtatgct aaagaccatg
     2041 taaaatctac cgttat
//
LOCUS       DAXWLF010000237         2053 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXWLF010000237 DAXWLF010000000
VERSION     DAXWLF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.243"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(102..709)
                     /locus_tag="ACGE4U_002658"
                     /pseudo
     CDS             complement(102..709)
                     /locus_tag="ACGE4U_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1232..1906
                     /locus_tag="ACGE4U_002659"
                     /pseudo
     CDS             1232..1906
                     /locus_tag="ACGE4U_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
BASE COUNT          735 a          327 c          356 g          635 t
ORIGIN      
        1 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
       61 ccatcagtaa aattacttgt catactattg ataaggagta tttaattgaa gagaatgatt
      121 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
      181 tctttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
      241 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
      301 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
      361 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
      421 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
      481 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
      541 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
      601 tagaagcagt tttgttttgc catccctctt tttggtttag tgtgctagta atataagcac
      661 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
      721 cctttactat ctatgacaag gacaacagtt ttaacataca gacattcatt aaatttggat
      781 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagaca gtcaacttaa
      841 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
      901 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tcttaaaaga
      961 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
     1021 tgaaaacaac cctagaaata tataactagt ccaatgaaaa aaaagcgaat gccgtagaaa
     1081 acaaggattc tgcgtacatt catatctcat tttgcgagca tatctgctat tactgtgact
     1141 aaatgttaat atggttaaaa tgaccgttat attttttata taaatatcgt ataattagta
     1201 tgaatataga agaaggaaag aaggtgcaat cttggaacct aaagacaagt atttagatta
     1261 cgctagggtc aataaagata aatttattga aacaatcatc aagggaaaaa tccctgatga
     1321 tgaaaaagat gcggtattca tggctggaag cccaggagca ggtaaaacag aggttgcact
     1381 aggtttagct taaaattatg acaaccatgt tgtgattgat gcggactatt ttagagcaca
     1441 atttccagaa tacaatggaa agaactcaag tgtttttcag aaagcttctt cttggttagt
     1501 tgaacaatca ttaaaatatg tactggaaca tggctattct ttcatactag aggatacttt
     1561 tgctatcctt agtgcagaga aaaatattat tcgagccctt aaaaataact ttcgcgttac
     1621 gattttctat gtttaccaag atcccaaagt tgcctgggat ttcactcgaa aacgtgaact
     1681 agctgaagga cgtcatgtgc caaaagaaat gtttatcaat gcgtttttta agtccagaga
     1741 gaatattgaa aaagtcaaaa atagacatcc tgaagtagtc cttcacataa tgattaagga
     1801 ttatcaaaac gacatttctg aagtgcatta tgcagcagat aatattcaat tagtactacc
     1861 aatacaatat acttcaaaag agttggagga ggaattacat gactgagaaa gaacaagtac
     1921 aacaaatcgt gaagaagtat aataaaagta tcgctgattt atcagaaaac gcctcagcga
     1981 aagaatttaa aacagtaatg aattacgtag cagatgaagc aaatcgtaag cagcgaaaac
     2041 tagttgggtt gga
//
LOCUS       DAXWLF010000238         2052 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXWLF010000238 DAXWLF010000000
VERSION     DAXWLF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.244"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(223..1224)
                     /locus_tag="ACGE4U_002660"
     CDS             complement(223..1224)
                     /locus_tag="ACGE4U_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFF8394748.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(1383..1649)
                     /locus_tag="ACGE4U_002661"
     CDS             complement(1383..1649)
                     /locus_tag="ACGE4U_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8394749.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1639..1995)
                     /locus_tag="ACGE4U_002662"
     CDS             complement(1639..1995)
                     /locus_tag="ACGE4U_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8394750.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          619 a          380 c          244 g          809 t
ORIGIN      
        1 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
       61 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
      121 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
      181 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
      241 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac
      301 agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat
      361 caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc
      421 cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt
      481 gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga
      541 attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg
      601 atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt ttttgttaat
      661 tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt ctaaattatt
      721 ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga tattgtcttt
      781 gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct taacttctat
      841 atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc ctacctcgcc
      901 tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat aattatcaac
      961 tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt ctacaatttg
     1021 tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg cattcgtttc
     1081 ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat tccatctata
     1141 tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg ctataaatac
     1201 ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc tttaaagtaa
     1261 tttttgtata aaatttattg acttttacac tataggacaa cttttatcta tttagtacaa
     1321 aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt ttcaactcgc
     1381 ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc atcaagttta
     1441 tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt tgtatgtgct
     1501 ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata aagagcaacc
     1561 gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt gtctttttgc
     1621 ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa gttcttttgc
     1681 atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat gggtaatgac
     1741 ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc ctaccttact
     1801 tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt gagctactag
     1861 catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat ctttttttgg
     1921 taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa atgtttcaaa
     1981 agatattttt tccataccca attatatcat atattgattt tatattatag tatataacat
     2041 aaaaattcga at
//
LOCUS       DAXWLF010000239         2031 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXWLF010000239 DAXWLF010000000
VERSION     DAXWLF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.245"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(406..1509)
                     /locus_tag="ACGE4U_002663"
     CDS             complement(406..1509)
                     /locus_tag="ACGE4U_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFF8394751.1"
                     /translation="MANKNGYDIEMSYVVAPGETLKEVAEELGLSHKDLAERLGITPK
                     TISKIVNGTAPITPETALKLERVLGISSNFWNQLELNYREKLTEIEAEAKLKDEIEIL
                     KEYIPYNEIANAGFVPKTRNLKEKVENTLNYFGYSSLKIFIESVNDNQLLAGAYRIKE
                     VDKVNKLALLAWIRAGELEAQEIEAEIFSKEKLKNSLSELRNLTLQSNPSIFIPKLQK
                     ICASFGVKVVFIPEVKGSKVCGLTRWLTPKPQAIIQLSLRYKSNDNLWFTFFHELGHI
                     LKHNKIPFYTSSNEYIDSIEEQEADEFSANTLIPRDSYNDFVEQGCFTRNSICNFAEM
                     ISIHPGIVLGRLQKEKYVQWNQYNDLKTWYSWK"
     gene            complement(1499..1828)
                     /locus_tag="ACGE4U_002664"
     CDS             complement(1499..1828)
                     /locus_tag="ACGE4U_002664"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8394752.1"
                     /translation="MIVRARKRKLQKILADSKLINLEFGKQIGDKVKQRIAELLAAET
                     LEDISHLPPTKLHSLSGEYEGCFAVKLTGNFRLVFKAYTCEEEFTTQKSNTEIIVIEE
                     VVDYHGK"
BASE COUNT          614 a          392 c          284 g          741 t
ORIGIN      
        1 agctttagct tgtataatac tcttcaaaaa attctaacac agaacaattc aattggctac
       61 attaatgtac cctgtaggac tcgagcctac gaccggacgg ttatgagccg tctgctctaa
      121 ccaactgtta atcagtgttc caaagggagt gattatactc tgctattttc aaactttctt
      181 aaatcgtcta tgataatctc agtttatggt ggcaaaatgg tggcaagaaa aaaagtgagt
      241 atgcccgcta cttgcgcaat cggagtactg ctcgatcatt tatttggaaa caggctacac
      301 ttgagggttt ggaagagtca aagcggttga gcggtgaacg gaaaaagaac ttaactacgt
      361 atagttaggt tcttttcgtt tatgttttat cggtagaata attttttact tccaagaata
      421 ccaagtcttc aaatcattat attgattcca ttgtacatac ttttcttttt gcaaccttcc
      481 taaaacgatc cccggatgaa ttgagatcat ttccgcaaaa ttgcaaattg aatttctagt
      541 gaaacaacct tgttcaacaa aatcattata gctatctcta ggtattaatg tattagcaga
      601 aaattcatct gcttcctgtt cttctatact atcaatatac tcatttgaac tagtataaaa
      661 aggaatttta ttatgcttta atatgtggcc taactcatgg aaaaatgtaa accataaatt
      721 atcattagat ttgtatctta aactaagttg tattatagct tgcggtttgg gggttagcca
      781 ccttgttaac ccacacactt tactcccttt tacctctgga atgaatacta ccttcactcc
      841 aaaactagca caaatcttct gtaatttagg aataaatata ctaggattgg attgcaaagt
      901 aagatttctc aattcagata aagaattttt taacttttct ttagaaaaaa tttctgcctc
      961 aatctcttga gcttctaatt ctccagctct aatccacgcc aacaaagcaa gcttatttac
     1021 tttatctact tctttaattc gatacgctcc tgctaacaat tgattatcgt taacactttc
     1081 aataaagatc tttaatgaag aatacccaaa ataattaagc gtattttcga ctttttcctt
     1141 aaggttccta gttttaggaa caaatcctgc atttgctatt tcattatatg gtatatattc
     1201 tttcaaaatt tcgatttcat ctttcaactt ggcttctgct tcaatttctg taagcttctc
     1261 tctgtagtta agctccaatt gattccaaaa atttgaagat atgcctaaaa ctctttctaa
     1321 ttttaaggct gtttcaggtg taattggcgc agttccgtta acgattttag aaatagtttt
     1381 cggtgtaatc cctaacctct cagctaaatc tttatgagat aacccgagtt cttcagctac
     1441 ttctttcaat gtttctcctg gagcaactac ataggacatt tctatatcgt acccattttt
     1501 atttgccatg ataatccacc acctcttcaa ttactattat ttctgtatta cttttttgtg
     1561 ttgtaaattc ttcttcacat gtataagcct taaaaactag cctaaaatta cccgtcaatt
     1621 ttacagcaaa acacccctca tattccccag agagagaatg gagtttcgtg ggcggaagat
     1681 gcgaaatatc ttctaatgtt tctgccgcta gcaattcagc aattctttgc ttaactttat
     1741 ctccaatttg ttttccaaat tctaaattaa ttagcttgga atcagctaat attttctgta
     1801 attttctttt cctcgctcta actatcatcc gacttaacct tttaggttaa caactctaaa
     1861 tatagtatac cacaataatt tgtttttcaa gcactttatg atttacttaa agattaacct
     1921 aaattctttc gcttatagta gtttctcttt atagattatc aaaagttgta aagattttaa
     1981 agtcaatttt gtataaaaaa aacttttttc gttctatgta ctgcccctca a
//
LOCUS       DAXWLF010000240         2030 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXWLF010000240 DAXWLF010000000
VERSION     DAXWLF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.246"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..565
                     /locus_tag="ACGE4U_002665"
     CDS             <1..565
                     /locus_tag="ACGE4U_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8394753.1"
                     /translation="YLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(849..1514)
                     /locus_tag="ACGE4U_002666"
     CDS             complement(849..1514)
                     /locus_tag="ACGE4U_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8394754.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(1668..1817)
                     /locus_tag="ACGE4U_002667"
     CDS             complement(1668..1817)
                     /locus_tag="ACGE4U_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFF8394755.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            complement(1810..2019)
                     /locus_tag="ACGE4U_002668"
     CDS             complement(1810..2019)
                     /locus_tag="ACGE4U_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFF8394756.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
BASE COUNT          655 a          360 c          295 g          720 t
ORIGIN      
        1 ttatctagat tatattgcca atgaatataa aaatactttg agtaaacgag ctttgatcta
       61 ttatcaacga gatgtagaaa gagacctggc gatcaatgca ctgattttag gatctggatt
      121 gagactctcc gaggttataa atattaattt agatgaccta tctttagata agaataatgt
      181 ggtagtaact agaaaaggaa ataaaagaga tgctgtcaat atagcagcat tcgcaatgga
      241 atatttagct aattatctag ctattcggaa agaacgatat aaagtaacag aaaacgaaaa
      301 agctttattt ctagcaattt accaaggaga agccaagcgt atatccggta ttgcaatcga
      361 acggatggta gcaaaatatt caaaaggctt tagaattcaa gtcagccctc acaaactgcg
      421 tcatacatta gctactagac tctaccaaca gacaaattca ttggtattaa ctgcccaaca
      481 attgggacat tcaagtacca atacaacaac tttatatacc catatagaca atgcggctac
      541 gatcgatgca ttaaatgctt tatgagtata atgtataaat aggcggaaat aagaaacaac
      601 acgctagaaa agcctgtatt atagtttttc tagtgtgttt cttttggtgt gatcctactt
      661 ttgtttatca tttatttttt attatcgtaa ataggtgtcg gtttgatttt agaaaaatta
      721 gtttggatat aatctgtggg atcatttgtt atctctctca atgaatttag atacaaatga
      781 ccataagttc ctaaagtcgt ttgtacatca gcatgtttta acctttcttt aattcgaagt
      841 gcatcttttt attgtgatat aagcaaagaa gcatgactat gccttaatcc gtgtatgcgg
      901 attatatgaa caccaacgat cgttgcatgt cttctcataa tatgacggat tgtatatttc
      961 tgagtaggtg ttccattata agaaagtaca aacgacatgc ctcctattga aacttgacgc
     1021 tccttccaag cttttaaaaa ggtaattgta ttgtcgtcta gttcgattgt acgaatacta
     1081 gaagccgttt cgggttctac aaactcatag gttttggctc tcttataata gagtgtttta
     1141 ttcacacgca agtttttatt ttcaaaatca atatctttcc aagttaatgc agtggcttct
     1201 cccaaacgca taacggtcat aaaaagtaac cacatacaaa taaaaccaaa atgttggtac
     1261 aaatcatcaa tataaaaagt tgaaataact tttgtaaatt cttcttttgt ccaaaaatca
     1321 actgtagttt tccttttctt cacatttcca ataacttttg caggattttt ttcaagtagt
     1381 cccaaaacaa ctgctcgatc taatgacatc tgaaaaatcc catacatgtt tcttacataa
     1441 gaatttttat atttgctcaa taaattattt tgccactttt gaatgtcaat tggacgtatt
     1501 tctcttaact ccattttaaa gaaataagaa aagttttttt gattgtagat aaccgatttt
     1561 tatatgttct ttcttctact ttcccttgat aataaggtag gaataattta taaaaaaact
     1621 cctcaaacaa catagtactt tgagaagttt ctgcttcttt tccataatta gaaacaagtt
     1681 cggtatacgc tttttttctt ctttaactgt tttaacccct ctacgttttt ttgtactctt
     1741 ttccctgttc gtttgtcgaa tcccaaacta gcagtaaaat gatatttccc atttttatct
     1801 tttttaaaat taaccatgat tctgaaaatc ctcctctacc tctatcttca tacctaaaac
     1861 ttcttctaca gattcaacag gtactagtcc taatcttcta ttttcgtaat aggtatagct
     1921 ttttgaagcc ataattgctt ttgcctgtcg tatgattctc tcagactgat acttcccata
     1981 acctaatttc attaattcat tttttgttat aaccaccatt taaccaccac
//
LOCUS       DAXWLF010000241         2017 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXWLF010000241 DAXWLF010000000
VERSION     DAXWLF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.247"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..149
                     /locus_tag="ACGE4U_002669"
     CDS             <1..149
                     /locus_tag="ACGE4U_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8394757.1"
                     /translation="AFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGL
                     IFEN"
     gene            156..1769
                     /gene="guaA"
                     /locus_tag="ACGE4U_002670"
     CDS             156..1769
                     /gene="guaA"
                     /locus_tag="ACGE4U_002670"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFF8394758.1"
                     /translation="MRRTERAAYRTKFYSNLSSPTRFDEEPKIIVLDYGSQYNQLITR
                     RIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVL
                     GICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVT
                     QAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTG
                     DWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVL
                     LRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDE
                     ATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLF
                     KDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANA
                     GLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKI
                     SVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT          620 a          412 c          421 g          564 t
ORIGIN      
        1 atgcctttaa ggtgtcccac tcaaacggcg tcactgaggg gctgaataac aagatcaagc
       61 tgattaaacg aattgcgttt ggttatcgca acttttataa ctttcgggca cggatctatt
      121 tacaacaagg tctgatcttt gaaaactaaa aaaatatgcg gcgcacagaa cgcgccgcat
      181 atcggactaa gttttattca aatttgtctt caccaacacg atttgacgaa gagccaaaaa
      241 ttatcgtact tgattacggt agtcagtata accaactgat cactcgccgt attcgtgagt
      301 tcggtgtttt ctcagagttg atgagccacc gcattaccgc agaagaagta aaggcaatga
      361 accctaaagg aatcatcctt tctggcggac caaacagtgt ttatgatgag aaggcctttg
      421 gtattgatga aaagattttt gatttgggga tcccagtttt aggaatctgt tacggtatgc
      481 agttgattac ttataaatta ggcggaaaag ttaaaccagc caaaaaccgt gaatacggaa
      541 aagcagaatt ggaaatcact tctgcagaag cggccctttt ccaaaacact cctgaaaaac
      601 aaaccgtttg gatgagtcat ggtgacctag ttacccaagc accgactgat ttcgatgtcg
      661 ttgctaccag caaagattgc cccatcgcat cgatccaaaa tgcagaacgc aaaatgtacg
      721 gaatccaatt ccatgcagaa gttcgccatt ccgaatatgg aaatgacttg ttgcgccatt
      781 ttacactaga tgtctgccaa tgtactggtg actggacaat ggataacttc atcgacatgg
      841 agatcgcaaa aattcgtgaa caagtcggtg acaaaaaagt tttattaggc ctttcaggcg
      901 gagtagattc ctctgttgtt ggtgttcttt tacaaaaagc gatcggcgat caattaacat
      961 gtatcttcgt tgatcacgta ttgttgcgta aaggtgaagc agaacaagtt atggaaagtc
     1021 ttggcggaaa atttggcttg aacattataa aagtcgatgc caaagaacgt ttcttatcaa
     1081 aacttgctgg cgtgtctgat ccagaagaaa aacgtaaaat cattggaaat gaatttgtat
     1141 atgtcttcga tgatgaagca accaaacttg ccggtgaaga aggcgtttct ttccttgcac
     1201 aaggaaccct ttataccgat gtgatcgaat caggaactga aactgcccaa accatcaaat
     1261 cacatcacaa tgtaggtgga ttacctgaag acatgcaatt caagctgatc gagccattga
     1321 acacattgtt caaagacgaa gtacgtgaat taggtaccca attaggcatg cctgacagca
     1381 tcgtttggcg ccaaccattc cctggaccag gcttaggtat tcgtgtcctt ggtgaattga
     1441 cagaagaaaa attagaaatc gtccgtgaat cagacgctat cttgcgcgaa gaaatcgcca
     1501 atgccggttt agatcgcgac atctggcaat acttcaccgt cctaccaggc attcgctctg
     1561 ttggggttat gggagatggc cgtacgtatg actacactgt aggtatccgt gctgtcactt
     1621 ctattgacgg catgaccgct gactttgccc gtatcccttg ggaagtcttg caaaaaatca
     1681 gtgtccgtat cgtaaatgaa gtcgcacacg ttaaccgtat cgtgtatgat attaccagca
     1741 aaccacctgc aacagtggag tgggaataaa gagccaacat aatatggatg aggaatacct
     1801 ataaatcaac attattcaat tactaaagtt cctgattttc gtaaaaaata agatggtttc
     1861 ctaccatttt ccctaccaaa gcccttgtta gattaactac taacaagggc tttgctttta
     1921 ttcactacct ttattttcct attgaattag ctttttatct aatcagattt ttagtactat
     1981 aaatatatat atcagatttt tcgatttaaa agatctg
//
LOCUS       DAXWLF010000242         1988 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXWLF010000242 DAXWLF010000000
VERSION     DAXWLF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.248"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            271..837
                     /gene="amaP"
                     /locus_tag="ACGE4U_002671"
     CDS             271..837
                     /gene="amaP"
                     /locus_tag="ACGE4U_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8394759.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            850..1038
                     /locus_tag="ACGE4U_002672"
     CDS             850..1038
                     /locus_tag="ACGE4U_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8394760.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1051..1608
                     /locus_tag="ACGE4U_002673"
     CDS             1051..1608
                     /locus_tag="ACGE4U_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8394761.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1640..1882
                     /locus_tag="ACGE4U_002674"
     CDS             1640..1882
                     /locus_tag="ACGE4U_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8394762.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          722 a          307 c          406 g          553 t
ORIGIN      
        1 taaatagaat cttgtaataa taactgagca taatctttgc aatttttaat cttgaataat
       61 atcctttaag tatagccagc aatcaaaagt aaatagagaa taagtaagca attgttattt
      121 cagtatcaga ctggcaataa aacgccgtta tgatccagca atgtttggta gcgctttatt
      181 tactgatgct atgctaactg ttcgctatct atactgtgat tgtcggtaga gttatttaca
      241 gggtaaaaat aagtttatag gaggtggaaa atgaataagg ggttaaaaat aatcggtgtt
      301 atcatgagtt tactgctact ttcagtattt ctagcgattg cgttgatcaa tgcgccgtat
      361 attatgcctg aacaattgga acgatttcgc ttctttactt taacgaatta ctacatgcaa
      421 caatatattt tctgggcagc tgtagtattt gccgttcttg ctattatcat cttgctagtc
      481 gttcttttct atccaaagtc aagaggaact ttcgttatga aacgggaaga tggcaaacta
      541 acaatcgaca aaaaagcgat cgaagggttg gtacgttccc atttacacga agaagagttc
      601 attcattcac ctaaagtacg gatacgttca acgaaaaatc gaatccatat ccatgtcaac
      661 ggagacttga aacgaacttc ttcattagta gggaaaacag gtgtattgat gcaagatatc
      721 gaagaagaag taactaaagt actcggaaca aaagaaacgg tcaaagtggc agtcacatac
      781 agcggttatg aagaacaaga agatacaaga gactacaagc actctcgagt ggaataggag
      841 ggccaaataa tgaaagaact attttcagca tacaagctcc ctattatctt tgggggtctt
      901 ggactactgc tagctgtttt atggttaacg attggatttt tcaagacatt gttgatcata
      961 atcatgacag caattggcgc aggaatcggt ttttatttga aacagtcagg aatagttgat
     1021 caatttttta aatcatagga ggaaacaaag atggaaaaca aaaacatgaa tacaacaaat
     1081 ggatcagttt tggcaaatga aaaaggagaa aaccgcatga tgaatggtga attgacttat
     1141 gaagacaaag tcatccaaaa aatcatcggt tatgcattga aagatgtaga tggtctattg
     1201 acattagatg gcggattttt ctcaaatatg gctgaaaaat tagtcaacac agataacgta
     1261 acagcaggta tcaatacaga agtaggtaag aaacaagtag ctgtggatct agatgtgatc
     1321 gtagaatacg gtaaagacat cgaagatatt tacaaccaaa tcaaagaatt gatcagtact
     1381 gaagtaaaca atatgacaca tttagatgtt atcgaagtga atgtgaatgt tgcagacatc
     1441 aaaacacaag aagaatacca aaaagatcaa gaaacagtac aagacaaagt aacagaagca
     1501 gcaaaatcca caggacaatt tgcttcacgc caaacagata aagcaaaatc tgctgtaggc
     1561 aaaggtgcac aaaaagtcaa agaaaataac gaaccacgtg tacaataaga agtaagaagt
     1621 cagttaggag gaatttaaaa tgggatttat ttgggcatta atcgtcggcg gtatcattgg
     1681 agcaattgca ggagcaatca cgaataaagg atcatcaatg gggatcatcg caaacatcat
     1741 tgctggttta gtaggttcag caattggaca agctttacta ggaacttggg gaccaacaat
     1801 tgcaggaatg gctatcattc catcattgat cggtgcaatc attttagtat tagtcgtttc
     1861 attctttgca cgtaaattat aagcaaattg aagaaaaact tatgttgaaa tgaagaacaa
     1921 atttgagaac cgtagcgaca ctcgctgcgg ttctttttta tatattttat atgaagtatt
     1981 ctttattc
//
LOCUS       DAXWLF010000243         1976 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXWLF010000243 DAXWLF010000000
VERSION     DAXWLF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.249"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(304..918)
                     /locus_tag="ACGE4U_002675"
     CDS             complement(304..918)
                     /locus_tag="ACGE4U_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8394763.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1524..1901)
                     /locus_tag="ACGE4U_002676"
     CDS             complement(1524..1901)
                     /locus_tag="ACGE4U_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394764.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          652 a          394 c          302 g          628 t
ORIGIN      
        1 aacgtgtatt aagagacaag aaagaatcca tatggattct ttcttgtctc tttttccact
       61 actttttatt tttctgacac tacatattct tataactcat ttctcataac tgtgaaacaa
      121 cttataaaaa taacaaactt taaacaaaac aagcttaaaa tcaaagatga tccatcttaa
      181 aataactaaa acaaaaaaag tagtcgttgt taatcctcat taataatcgt tttctttttt
      241 tcaaacgctc catttttatg tggaacatta tttttttaag gaactttgtc aacatgtgga
      301 taactatttt ccacttgtgt cctggactag ctgttttgct gtaaaataca aaacttgctg
      361 cagcaaaaag atcgttgcaa gagaggctgt tccgcagatc gctggatcta gatttggcaa
      421 agtcaagact gcatcatgta aattggcgtg gttgtccaaa accaaatcag caaattcaaa
      481 taatcgcaaa ccactgctat gttgcgattt gatacttcgt gacaaatcaa atccagtaac
      541 aatgattaat ggatgaccat tttctttgat ccactgagct agttcgatga ttgccgggtt
      601 tcttccttca ttcgaaagaa gaagaaaaat gtcttctgat ttgacagaaa gctgatcaat
      661 aattgcctgt cctgcacctt ctagcgtttc atagacacct ccggctggat tttcaatcaa
      721 tatacttggt acaagatccg gggtttgatc ccaaaattca aatgctacac cacttagtgt
      781 gcggctagca tatatctgca atctaccacc ttgattaatt tgtttcgcga tccaagtgcc
      841 agcttgaagc atcagcggct cttctatcct taatttctct tccaattggt gataatagtc
      901 aaaataacat aaactcattt atctctcccg cctagctata cttgacatga atagaaacag
      961 ctgcctaatc taaggaagta cataaaaaaa cagtagttag acagtaacac atgtggtctt
     1021 tgctgtttca aacacaaaaa aggcattgaa cgaatacgcc caataccttg acatagcaaa
     1081 taaaacaacg tgcagaccat ggaaaatatc tacatagggg tagaattttc accctagatt
     1141 aggtacaata aattaggtcg atgcactgca ttgactatgg caaaatgatc ggtcagggtg
     1201 tatgtgtgtg actggtcatt ccctgattct tagcgccaac taagggtcag ggagcctttt
     1261 tttattcagc tgtttctatc tgaatttatt gtaagtaatc tcttatccaa gtgcaagcaa
     1321 gaaaatacaa aaaaacattg gtatgattta tatatatata aataacataa aaaaaggaat
     1381 ggcttatccc gatacaacaa gcattccttt aaaaaagatt cttttatata agtgaaaaaa
     1441 gcttttttcc accaattaaa taaatctctc atctcaatag tttatccatt attttatctt
     1501 tttttgcgta tacctactaa atattaccgt cttcttctcg ttcttctact ttgttcgaga
     1561 gaatctgtac tagaaaaagt agttgtatgt ctaccttttc tgcctatccg agactttctt
     1621 ttcatatatg ggatataaat gattagatac agtacagcaa aaaataaagc aaatacgcaa
     1681 gcttcccaac tacctaacgc caattcaacc atcaaggtag caaccccaac ggatgctgca
     1741 taccagataa aaatcagaaa cgtttctttt tttctgacca tgcgaaacat gataaataga
     1801 ctaataagca atagtggcaa cacaaatatc caattgcttc cttccatcag ccacttgagt
     1861 atatcaagca gcgactgtgc aacagcgact aattttccca tagtgaacac acaccttttt
     1921 ttataattaa aaaattcgct tttttattct actatgaatc ttctggcttg gctttc
//
LOCUS       DAXWLF010000244         1973 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXWLF010000244 DAXWLF010000000
VERSION     DAXWLF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.250"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..237
                     /locus_tag="ACGE4U_002677"
     CDS             <1..237
                     /locus_tag="ACGE4U_002677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394765.1"
                     /translation="RKDTLKSFRINLPNKFIGGFKMSHTLLIKDTLNILDLNINIHFE
                     ENCLTKEKYKGQICLIYKGTLQYTPEEYPHLPIY"
     gene            297..530
                     /locus_tag="ACGE4U_002678"
                     /pseudo
     CDS             297..530
                     /locus_tag="ACGE4U_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(578..697)
                     /locus_tag="ACGE4U_002679"
                     /pseudo
     CDS             complement(578..697)
                     /locus_tag="ACGE4U_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
     gene            complement(1517..1660)
                     /locus_tag="ACGE4U_002680"
     CDS             complement(1517..1660)
                     /locus_tag="ACGE4U_002680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394766.1"
                     /translation="MENAITVITNSFFSSIKYLKTLFVCQADQCSFRLGRTLISSQVM
                     IEV"
BASE COUNT          703 a          376 c          262 g          632 t
ORIGIN      
        1 agaaaagaca ccctgaaatc ctttagaatt aatttgccca acaaattcat aggaggattc
       61 aagatgtctc atactctgct tatcaaagat accctaaata ttctagacct aaatatcaat
      121 atccatttcg aggaaaattg tttaactaaa gaaaaataca aaggacaaat ttgcttgatt
      181 tacaaaggga ctcttcagta cacaccagaa gaatatccac atcttccaat atattaaaag
      241 aaccttcttg aatcattgag tgaaagaaaa gagattcgtc actatttgag gactgtttaa
      301 ctagagacat tagcgatctg cctgaaacct atacaaccac tttacgaacg tttaaaaatt
      361 ttctgccaca gattcaaaat gcgcttaact gcagctattc aaacagacca ttggaatgtt
      421 taaacaacca catcaaagta ttaaaacgca atacgtatgg ctttcgcagc ttttacaatt
      481 tcaaactacg gatcatgatt cgtcatggca aaacattttt aaccaaataa acagaagaat
      541 gtgggataaa tctaaaaatc agttttgcta cagactctcg agtaaacggc gggagcagaa
      601 gcaactcctt cggaaataag ccgaaattca caaaaatttg aaaagcaatt ttcgtgaatt
      661 tcttcttatt tctcggagtt aaacacttct gtcccagcct cttctgtcca aattattcta
      721 agatttctct ttaccaacat catttgacaa agagcttctt ttttgaatac tatattcgtt
      781 ctgtttttca tcttcaaata cgaactcatc cgcagaaata tttgtcacaa ctccatgaat
      841 cttcttttca ttaccaccta tataccacag gattcctaca cagaaacaac aaaaaaatag
      901 cataaaatag gttttcttta ttttatatcc ctccttttag attaaaagct cacagaaaac
      961 gctttctaaa aagacaatag aataatcata atttttcatt ttgtattatt tatacaaata
     1021 atacgcatgt tactgaaaaa tgatatggtt ccctactttt tgagataagt taaataaaat
     1081 ctgtcatcga taaaacagct agtacagtaa tgataaatag taagaactta aaatattttg
     1141 gatttttatt tttgaaataa tcgaagaaaa gtaaaagtga taacgaaata gcaaacaaga
     1201 ttatatctat aagattattc attgagaaat cgccccatat ggaactctat tttgacctga
     1261 cacaccgaga cataaagcaa tcatatcctc tgcattgttt tatactatta gaactatagc
     1321 tacacctatt ggatgctact ggctctaaga ttaatgctaa tacagtaaca tcaataccta
     1381 aactagcaga aactctattt gcatcttcaa tttcagatgc ctggactgtc gtgactagcg
     1441 ccgtagtaga aaaatttact ggtactaaca aagatgcaga cacgactttt atttcactta
     1501 tcctttatcc cataacctaa acttcaatca ttacttgtga gctgatcaat gttcttccaa
     1561 gtctgaaaga acattgatca gcttggcaaa caaataatgt tttcaaatac ttgatcgatg
     1621 aaaaaaagct attggtaata acagtaattg cattttccat tttcgtgtca gatacttcgc
     1681 ttacagctgg taaacgatca atcccattgt caaattttgg atttttttct gatgatgtct
     1741 tgctttagtt cataaaataa tccagcaata aattcctcgc aaggatagcc aacttcattt
     1801 tcctaatcgg aatttttaaa cttatcggta tagatacaga tgcaatgaga acaaacattt
     1861 atttcgagtc accatctaaa atattttcct ccatgtaccc gttcattatt atttagttat
     1921 caccaaagat atctctggta taaacttttt cttcaacatc ctttaaaata tct
//
LOCUS       DAXWLF010000245         1956 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXWLF010000245 DAXWLF010000000
VERSION     DAXWLF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.251"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACGE4U_002681"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACGE4U_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFF8394767.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT          700 a          387 c          353 g          516 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaat
//
LOCUS       DAXWLF010000246         1931 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXWLF010000246 DAXWLF010000000
VERSION     DAXWLF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.252"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            498..1058
                     /gene="amaP"
                     /locus_tag="ACGE4U_002682"
     CDS             498..1058
                     /gene="amaP"
                     /locus_tag="ACGE4U_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8394768.1"
                     /translation="MRKRLKLLLILVLFFVFFPLAHTLITMQSVVSLPFYLLPFTSYP
                     YIGNSMSQFLFWVSVCLLILLFIILIIVLFYPKANNYIEKKTASGKIAIQKKAIENFV
                     LIAAKEEPFIVDPTVKSVIKKKKIKIYIRGKIRKAFQTQERQTAFIRKVNTNLNELFG
                     AEQTILTEIIFKDYHEKNTNTKSKVL"
     gene            1068..1250
                     /locus_tag="ACGE4U_002683"
     CDS             1068..1250
                     /locus_tag="ACGE4U_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8394769.1"
                     /translation="MAEFISKYKYPFIFGCLGLLLGILIFTLGFIKTVILTVLTICGF
                     YLGNYLEIVGFFDKQN"
     gene            1306..1884
                     /locus_tag="ACGE4U_002684"
     CDS             1306..1884
                     /locus_tag="ACGE4U_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8394770.1"
                     /translation="MENKTNNTKTEIKKNDVAKTSEIIKGELVFEDKVIQKIIGIALD
                     EIDGLLTIDGGFFSNIAGKLVNTDNTTSGVDVEVGKKQVAVDLSIVAEYGKDVTTIYD
                     KIKEVISKEIKKMTGLDVIEVNVDVVDVKTKEQHENDSVTLQDHLSDAASTTGEFASK
                     QFEKSKEALGVASEKVSDGVENVKEASEPRVE"
BASE COUNT          751 a          221 c          310 g          649 t
ORIGIN      
        1 tattttttat cgaaaaaaat tctaaataga gtatttttca tttccgaaca aattatgatc
       61 ctttcttgac aaatatagaa gctacaatgt ttactagttt tgaataagaa agatagtgct
      121 gtgtctttta taattgattg tttgattttt catgaaaaaa tgctcgagtt aaaaaaacaa
      181 attgaacgtt cattttattt gacttagtag ctccttcttg ttaacattgt ttcataaacc
      241 atttaatttt ataaattaag tatgtcttta tctttctgaa atgtgagatg ctactttaaa
      301 attttaggga tattaattga ttaaatttat tgtattaatt tacctaaata agtcagacaa
      361 ctgaaaggag catagattat agaaatcaaa gcaccacaac ctttggagat tttttaatag
      421 atatatattt tatcgtaaat tttcaaggaa tgataaagta agcgaatttg ttcaaaacgt
      481 agataaagag gtgtattatg cgaaaaagat taaaattatt attaatcctt gttttatttt
      541 ttgtattttt tcctttagca catacattaa ttacaatgca aagtgttgtt agtttaccat
      601 tttacctact tccatttaca agctatccgt atattggtaa tagcatgtct caatttttgt
      661 tttgggttag tgtatgttta ttaatactat tgtttattat tctaattatc gtgttattct
      721 atcctaaagc gaataattat atagaaaaaa agacagctag tggaaaaata gcaatccaaa
      781 aaaaagcaat tgaaaatttt gtattaattg ctgctaaaga agaacctttt attgttgatc
      841 caacagtaaa atctgtaatt aagaaaaaga aaatcaaaat ctacatacga ggtaaaatac
      901 gtaaagcttt tcaaacgcaa gagcgccaaa cagcatttat aagaaaagtg aatactaatt
      961 tgaatgaact atttggtgca gaacaaacaa tattaaccga aataattttc aaagattatc
     1021 atgaaaaaaa tacaaatact aaatcaaaag ttttatagga gaaagcgatg gcagaattca
     1081 tatcaaaata taaataccct ttcatttttg gatgtttggg actattgtta ggaatattga
     1141 tttttacgtt aggatttatt aaaacagtaa tattaacagt gctgactatt tgtggttttt
     1201 atttaggtaa ctatttagaa attgttggtt tttttgacaa gcaaaattag ttaataaccg
     1261 tttttacggt aagaaataaa tttctaattt ttaggaggaa aaataatgga gaacaaaaca
     1321 aataatacaa aaacagaaat caaaaagaat gacgtggcaa aaacttccga gataattaaa
     1381 ggagaactag tttttgaaga taaagtaatt caaaaaataa ttggtattgc attagatgag
     1441 attgatggac ttttaacgat tgatgggggc ttcttctcaa atatagctgg aaaattagta
     1501 aatacagata acacaacttc tggagttgat gttgaagtag gaaaaaaaca agtcgcagta
     1561 gatctttcaa tagtggctga atatggcaaa gatgtaacta caatttatga taaaataaag
     1621 gaagttatct caaaagaaat taagaaaatg actggcttag atgtaattga ggttaatgta
     1681 gacgtcgtag atgtaaaaac gaaagagcaa catgaaaacg attcagttac actacaagat
     1741 catctttccg atgcagcttc tactactgga gaatttgctt caaaacaatt tgaaaaatcg
     1801 aaagaagctt taggagttgc aagtgaaaag gtaagtgacg gtgtagagaa cgtaaaagaa
     1861 gcatctgaac ctcgcgtaga ataacaaatc ttttttattt aatgtacaat ctataaaatt
     1921 aaaagactgt a
//
LOCUS       DAXWLF010000247         1912 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXWLF010000247 DAXWLF010000000
VERSION     DAXWLF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.253"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            2..1318
                     /gene="brnQ"
                     /locus_tag="ACGE4U_002685"
     CDS             2..1318
                     /gene="brnQ"
                     /locus_tag="ACGE4U_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFF8394771.1"
                     /translation="MGSMLFGLFFGAGNLIFPVHLGQEAGAATFWANLGFLVTGIGLP
                     FLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPRLATTSFEIGLAP
                     FIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLVLLGALLLLAFLN
                     PLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTIQNMGVTKPAEIA
                     KDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIADHYLGTYGSILL
                     AFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLAANVGLTNIIQFS
                     LPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDGLNACPETIKQTP
                     IVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD"
BASE COUNT          532 a          344 c          367 g          669 t
ORIGIN      
        1 tataggctcc atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca
       61 tttgggtcaa gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg
      121 tatcggtctt cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata
      181 tgaattagca aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt
      241 agttatcgga ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt
      301 agcaccattt atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt
      361 ttttgtgctg gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa
      421 gttcctgaat cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa
      481 tccactaggt tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt
      541 cacagggttt actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat
      601 catcattgtc acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga
      661 cacgatcaaa tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc
      721 ttatttaggt gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct
      781 ggctcagatt gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt
      841 tatattagct tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac
      901 agatttgttt ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg
      961 tttagcggca aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt
     1021 catctatcca ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca
     1081 gcgtacggct gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg
     1141 gttgaacgct tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt
     1201 tgcggaaagc tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat
     1261 cggttttgtt atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata
     1321 aaagaggttg tgaaagagac gttcagcctt attgccgata ggctgtctct tgaacactgt
     1381 ttattgagag gtcgtgaaaa agccgctcag tttcgagtat taagaaatat tttttgaaaa
     1441 tggcttctca catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc
     1501 gtttattgag aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa
     1561 aatggcttct cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca
     1621 ccgtttattg agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc
     1681 tccatatact ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat
     1741 ttcttttata atatagattt tagacatata tatgatataa aaatagtttt cgcaaaaaaa
     1801 cttaacaact ttcgaaaact ttcgtcacaa aatattcaca actagtgact attatataag
     1861 cataccaaca aacaacccta acaacacttt ttcattttta actccttatc ct
//
LOCUS       DAXWLF010000248         1911 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXWLF010000248 DAXWLF010000000
VERSION     DAXWLF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.254"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            524..856
                     /gene="traE"
                     /locus_tag="ACGE4U_002686"
     CDS             524..856
                     /gene="traE"
                     /locus_tag="ACGE4U_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFF8394772.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1475..1816
                     /locus_tag="ACGE4U_002687"
     CDS             1475..1816
                     /locus_tag="ACGE4U_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFF8394773.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          659 a          258 c          412 g          582 t
ORIGIN      
        1 tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt aattgtctgc
       61 tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt ggacaattga
      121 aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc gtcagcgttc
      181 atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac ggtattaccc
      241 gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg ctgtgttaag
      301 gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat aaaaatgtcc
      361 gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt ttggatgcgt
      421 tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa aataacaaga
      481 aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat atacaggaat
      541 tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag agaaaatcac
      601 gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat atataatcca
      661 gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc aaataatttt
      721 aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt atgaccaaaa
      781 cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg
      841 gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt ttcgcttgct
      901 tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata aggagtagac
      961 ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga ttggtaacgt
     1021 aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg aatttgtgta
     1081 aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta gaatagcgtc
     1141 tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc atggaataag
     1201 gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca
     1261 tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg ctgtctacta
     1321 gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt ttaagtaaac
     1381 gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattgata taaaacatag
     1441 agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc cgaatatacg
     1501 attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag gatttgtgac
     1561 gtttagaagg ggcggaaatt gtaaaggatt agatgtttta gcattagacg aggatgattt
     1621 atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg aagatgatga
     1681 aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat tatctgttga
     1741 agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg aatttgtagc
     1801 ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt agaaggagtc
     1861 acgacgatta ataaaaaggt agtattacct taggcattga gtaaggaatt t
//
LOCUS       DAXWLF010000249         1882 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXWLF010000249 DAXWLF010000000
VERSION     DAXWLF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.255"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..1384)
                     /locus_tag="ACGE4U_002688"
     CDS             complement(<1..1384)
                     /locus_tag="ACGE4U_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFF8394774.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGV"
     gene            1510..>1882
                     /gene="tagD"
                     /locus_tag="ACGE4U_002689"
     CDS             1510..>1882
                     /gene="tagD"
                     /locus_tag="ACGE4U_002689"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HFF8394775.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQ"
BASE COUNT          714 a          311 c          264 g          593 t
ORIGIN      
        1 ttactccttc ttctttagat atagtttttt ggatagaacc cagatatccc caaaaaataa
       61 atgaacctat cgtactaacc aaaatgagtt ctgtgataaa taatgccgaa actgctattg
      121 taagaatcat taaaatagca aaaaagtgct tatcttttac tagatttggt tgtgtgaaac
      181 aataacgcaa ggctttaaag cattggataa ataagaaggt taacaatgtc aatagaccta
      241 atcctcctgt agcagccatc actaagaaaa taaaattatg agcagttaaa gtatcttttc
      301 ccattaatgt atatggtagt ttatcatgtg catattcaat atagtagcct ggagaacctc
      361 cggatatcgg cttactcttg aatatttcaa ttgcactttt ccaaagttca aaccttaaat
      421 tagaaacatc cccgctatca gtgtcagggc gttttaaatc aactttattg ctgctagttg
      481 ttcctgatga atcagatgta tcttttttta cttctgtaat atgcggaaca tgtgcccatg
      541 catgagcagt tatatctgtt acataataca tcgcaattat acttagcaat cctactacta
      601 atgcagttag ccatcttttt aaagtattag ttttaagaaa acgactacta taacttgtaa
      661 agaaactaaa aacaaaagtt gttattaata aacaaactaa tgtggtgcgt gaacccgata
      721 aagcaataaa cgagaattga ataaaaatat taattacaaa aaatatttta aatagaagat
      781 ttaaatgctt gtaagtgacc aaataatata tagcaataat gatgacgatt aatgatataa
      841 ccgcaccaaa atttggatct gtatatacac caaataaacg gttgcctaac caccctaaac
      901 gaactgggta atacttatat tttattggaa tttttatgcc atattgaatg acaaaccagt
      961 ataatgatac taaattagct aaaaaccata ccaatgaaag tatccaggta aatactttga
     1021 aaaaaaagtt actactatag tctttgccaa actgataaat cataaagaaa aagattaatt
     1081 gccacatgat taacttaata ttttcgctat atccatattt taaattcatt aaactgctta
     1141 ctagcatcgc gccaataaaa gcaattaata gccaatcata tttttttaga tttttttctc
     1201 taaatgcaat aattgcatct ataatgatca agacaactcc cgctattcca gcaggccaaa
     1261 aaagtaaagt atcatatcta gtaggtaata gtaaatacgg gcctattgct cgtaacatta
     1321 aaataaaacc aaaataaata aaataccaac ctttcacata ttttaaaaat ctttcattca
     1381 acatttttcc tccatccacc tatttctcat aaatatttct tatccttttt aggataaact
     1441 ggtcctttca attctaacaa aaaaatgcta aactaacaat gtatattaat gaatttcgag
     1501 aggtgaaaga tgaaaagagt aattacttac ggtacctttg atttgcttca ttacgggcat
     1561 atcaatttac tgaaacgagc aaaacaatac ggagactatc ttattgttgc cctttctaca
     1621 gatgaattca attggaatgc gaaacaaaaa aaatgttatt ttagttatga aaaaagaaaa
     1681 cagttacttg aagctattcg ctacgttgat ttagttattc ctgaagaaaa ttgggaacaa
     1741 aaagtaaaag atataaaaga ataccatgca gattatttcg tgatgggcga tgattgggct
     1801 ggacattttg atgaacttga acaactaaca gatgcacaag tgatttattt accaagaaca
     1861 cctgagatct ctacaactca aa
//
LOCUS       DAXWLF010000250         1868 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXWLF010000250 DAXWLF010000000
VERSION     DAXWLF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.256"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(312..767)
                     /gene="tnpA"
                     /locus_tag="ACGE4U_002690"
     CDS             complement(312..767)
                     /gene="tnpA"
                     /locus_tag="ACGE4U_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFF8394776.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1010..1868
                     /locus_tag="ACGE4U_002691"
                     /pseudo
     CDS             1010..1868
                     /locus_tag="ACGE4U_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT          591 a          361 c          354 g          562 t
ORIGIN      
        1 aaaggtgttc gataatccag tgattttcta ggaagattat ttctgcgcca gcattttcta
       61 atgtttgaat ttgtgttttg taatcttggt tgcttgtgct aattcttgaa tagccgtaaa
      121 ttgccaaaat ttcatcaccc tttctggaca taagtttctg acaccagtta aaatcaagtc
      181 tatcagtaac ccatacaccc gtgaggacgg gtgtattttt aatcccccta gaaggggtcg
      241 acactcgctg ccccttaaaa ggggattttt aaaagaccgc caactgctca tcttcactca
      301 cttacttctc tttatttttt aaatggttct acgtactctc ttttacttat aatatatcga
      361 atgcgatcat cagcttcttg ttcacggatg tatttttcat ttgtcttttg attcaatccg
      421 actgtactca catagtatct cttcgaccaa aaggatttgt ttccgtattt atatttgaga
      481 tttgcgtgct gctcgtggat tagtaccgca ctacgacctt tcagataccc cataaaactg
      541 gatatacata tttttggtgg tactcgaaca agcatgtgaa tatgatctag cattgcatgt
      601 gcttctatga tttccacatc tttcatttcg cacaacttcc taagtatttt tccaagatct
      661 tttcgtaatt gcccatagat tacttttcgt tgatattttg gtgtaaaaat aatatgatac
      721 ttacaattcc aacgagtata tgctaaactt ttatcgtcat ttgacatgaa aaaactcctt
      781 tcgatctgat gagcggttgg tagccactca ctagtatatc gaaaggagtt tttatcgcta
      841 aagatacttc ttccacgcgt agaaacgcgc ggttttcttg tttcgcacgc tttacgcaca
      901 aaactgactg aagtcattaa gaaaataaaa gggtctaaaa cttttaagtt ttgtaccgac
      961 ccccaaaagt tagaccaaaa aatctaactt ttggaggtcg gtatttttta tggctaagta
     1021 tacttttgaa cttaaattaa agatcgttca tgattacttg gatggtaaag gcggaagtga
     1081 ttatctggca aagaaatatt ccattaaggc tccatcacaa gtgaagcgct ggatcaatgc
     1141 ctatcaagaa tttggtgaag agggtctagt aagaaagcga caaaagaaaa aatattctgt
     1201 tcaatttaag ctggatgcga tagagttata tctaacaagt gagctgtcct atcgtgaagt
     1261 tgccaacaca cttaacatga acaaccctaa tttgatcgct agttggatgc gtcaattccg
     1321 tgagggtggg atcgatggac tctcaaaaac gaaaggatgt ccacctatct tgtctaagaa
     1381 aaatgaaccc aaaaataaga aaaaatctac aactaaagca acgtctaaag aacgtgaacg
     1441 catcgaggag ttggagaaac gagtccgttc ccttcaaatt gaaaatgcct ttttaaaaga
     1501 attgaggaag ctgcgaaaac aggaagccca acaacgacga atgaagcaat cgcacgaatc
     1561 atcgcaagcc tccgaggacc gtttaaatta gttgagcttc tagaaacttt aaagttccct
     1621 aaggcaatct ttatgtactg gcaaaaacga ttggaccgta aaaatccaga tcaagagatt
     1681 gaagcggaaa tgcttaaagt tcgggagaag cacaaggatt atggctgttt acggatgacc
     1741 aatgaactaa gaaaccgcgg tttctctatc aataaaaaga aagttcagcg cctgatcaag
     1801 aagctaggaa taaaagtaac gacatacaca cgtaaatcac ggcgctacaa ttcttatcga
     1861 ggacaagt
//
LOCUS       DAXWLF010000251         1849 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXWLF010000251 DAXWLF010000000
VERSION     DAXWLF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.257"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(57..758)
                     /locus_tag="ACGE4U_002692"
     CDS             complement(57..758)
                     /locus_tag="ACGE4U_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8394777.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(774..1214)
                     /locus_tag="ACGE4U_002693"
     CDS             complement(774..1214)
                     /locus_tag="ACGE4U_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFF8394778.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1214..1789)
                     /locus_tag="ACGE4U_002694"
     CDS             complement(1214..1789)
                     /locus_tag="ACGE4U_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFF8394779.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          518 a          404 c          285 g          642 t
ORIGIN      
        1 aggcttattc tacatcacgt agtaattttt ctttcccgtc gtttggtttt ttatttttag
       61 cctaattctt gaccttttgc atgctggtta tccgtaaaat attttccagc aggctttcct
      121 tccaattcag ctaatttttc ttggatctga cggatcacct tttttgcatc ttctggattt
      181 tctacaaaat cgaatttatc cccatccact tgtattttag gacatacatc aaaatcttca
      241 taccattgat tatagcgggt gttcaacgtt tcataatagg tatataacgt ctcatcgctt
      301 tctagctgtt catagtcacg cccacgtttt ttgatacgtt ctagcatcgt gtccaaagaa
      361 actttgatat ggaccatcaa atcaggccgt ttttttggag caacatcatc taactcgttt
      421 agcatcgtat ctaataagct atcgtactgc tgtacttcga tatccgttac ccgacctaaa
      481 tcagcgttta gatggaacaa caggctatct tcatagatgg atcggtctag cacattgtca
      541 tcgttcacta aagcattttt cattgctaaa aatcgcttgt tcaaaaagaa gatttgcaat
      601 aaaaaagcgt actgctcagg atttgaataa aagagcggca atacttcgtt atcttcgaca
      661 ttttcataaa atggtttaga atcaatctct tttgcaagca tatccgtcaa actgctctta
      721 cctgcaccaa tcgttcctga aatcaaaatc actgacatac aacccttcct cttctataag
      781 tactcgccta cgtaagtttc aacaggcaat gtttcaaaat catattcacg tacagcttgg
      841 tctgatttta aatacgtatg gagactttgt gtaatcatca agttcgtagg taccgtctct
      901 tcttgtacaa tgatcatcgg ttttttcaac atagttgcca cacctaattc gtaagccgtt
      961 cctggatcga ttgtttgatg ctcaaagtca aggatagcaa caacaaattc tgcatttgtc
     1021 agttcttcca tatctttttc gtatacttcc tgcgcccatc cagcgctgaa tagttcataa
     1081 ttgctttctt ggtgatgacg tggagagtag aaacttgtga ctgttttgtt ttcctccaac
     1141 gctttttcga tacgacttac tcgatcgatt tgctcctctg aaaaaaatgg tcctgctaaa
     1201 tatatttgac tcattatttt gcctccttca ataataactg atttttaata gatactgctc
     1261 gtaattcatt gaccagttcc aatggattgt tggaaaagat tacttgatcc aaccattttt
     1321 tatccataaa atcctctttt gcaaaagttt ctaataattg ttgcaagccg ctgtaataac
     1381 cgccaatatt caatattccg attggcttgt cgggcgtcag accgattgct atccaagaaa
     1441 gaagttgtga gaattcctcc aatgtaccta gacctccagg gaaaacgagg acagcatctc
     1501 ccttttcaaa taataatacc tttctttcat cgatcgtttc tgtaggaata aatgtagtaa
     1561 catcttttcc aggcaattca gtttcaaaaa gcccattcgg atagatacct gtgacttttc
     1621 cgttattttc cagtactgtt ttactaatca ctcccataat cccggagtat gaaccaccgt
     1681 aaaccagttc gatttcttct tgtgccatgt attttcctaa cgtttgcgca ataaatttgt
     1741 aactttcttt attaccaaaa cgggaaccgc aaaaaacagt aagtttcata aaaaaacact
     1801 ccctaactca aaagtctatc ttcattctac acgtgttttt ttaaaataa
//
LOCUS       DAXWLF010000252         1795 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.258, whole genome shotgun sequence.
ACCESSION   DAXWLF010000252 DAXWLF010000000
VERSION     DAXWLF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.258"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..>1795
                     /locus_tag="ACGE4U_002695"
     CDS             <1..>1795
                     /locus_tag="ACGE4U_002695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394780.1"
                     /translation="TQEVDKDATVLSDTPRDLVNLEDTSTDIENVTPQKIGKKDSTSL
                     SEENLSSPASSILRRRTRSLDVQTGETFLEDWALPDNSNISGSLITQTGITYVGVKTD
                     DPTQLHLKMAYRAAATPAAIDWIYIRVDPELTKYIEEINIKNTVNIGAQVGYQRYFEK
                     VDANTLSDVNTFGTPTGVSDAYAQVDQTTGGIYRVLLDKFFTRIPGVKPKERGALSGN
                     WGVFPSVPLEAERYEANIYIKLSKDVAELQQELGRDKFGVQLRLQGQSSSGVATIAKE
                     SLNKNTIIDLSKQESIENNSNWLNSPSVHTITTTYEGKQMGSDGQFSNTLEGTDKSLI
                     RVQNTIMNNLLYGTALDLGRPVSFNIEIDPQILNLVSDDDYITIWQVYRENSINHHDG
                     GSNAPVMLKKNMFVDNKIKIEVNKDKAGQYENGVYYVNAVESFMNTVSSSPFVGANFI
                     DIPVMPKPLIEDEGQLVSAEIKTWFTDKKNDQERVVRNSLASSYINLFPTTINFYDYS
                     TKDTEQLLKLRESTYLVSTFGQEINGMDMGETYEISNYEFVTADPESLIITQKDGNVI
                     NLYYKKLSSDAEFYEPEVEKEEVNYGGTIDLT"
BASE COUNT          649 a          253 c          352 g          541 t
ORIGIN      
        1 atactcaaga agtagataaa gatgcgacag ttttatctga tacaccaaga gatttagtta
       61 atttagagga cactagtaca gatatagaaa atgttactcc acaaaaaata ggtaaaaaag
      121 acagtacgtc tttatcagag gaaaatcttt cgtcaccagc atcaagtatt ttacgcagaa
      181 gaactaggag tttggatgtt caaactggag aaactttttt agaagattgg gcactaccag
      241 acaattctaa catttctgga agtttgatta ctcagactgg aattacttat gtgggtgtaa
      301 aaactgatga tcccactcaa ttacatttga aaatggctta cagagctgca gcaacaccag
      361 ctgcaattga ttggatttat attcgtgtgg atccagagct tacgaaatat attgaagaaa
      421 ttaacataaa aaatactgta aacatcggcg cgcaagttgg ttatcaacga tattttgaaa
      481 aagtagatgc taatacattg agtgatgtaa atactttcgg aaccccgacc ggtgtaagtg
      541 atgcttatgc acaagtagat caaacaactg gcggaatcta tcgtgtgtta ttagataaat
      601 tttttacacg tattccaggt gtaaaaccta aggagcgagg tgcgttatct ggaaactggg
      661 gtgtatttcc atcagttcct ctcgaggctg agagatatga agcaaatatc tatataaaat
      721 tatcaaaaga tgtcgcagag ttacagcaag agcttggaag ggataagttt ggtgttcaac
      781 ttcgtttaca aggtcaatca agttctggtg ttgcaactat tgctaaagaa agtttaaata
      841 aaaatactat tattgatcta tcaaaacagg agtcaattga aaataattca aattggttaa
      901 actcaccatc tgtgcataca attactacta cttatgaagg aaagcaaatg gggagcgatg
      961 gtcagttcag taatactctc gaaggaactg ataaaagtct gattagggtt cagaatacaa
     1021 tcatgaataa tcttttatat ggaacggcac ttgatttagg tcgacctgtt tcatttaata
     1081 ttgagattga tccacaaata ttaaatttag tttcagatga tgattatatt acgatttggc
     1141 aagtatacag agaaaatagt atcaaccatc atgacggtgg atcaaatgca cctgtcatgt
     1201 tgaaaaagaa tatgtttgta gataataaaa ttaaaattga agtcaacaaa gataaagcgg
     1261 gtcagtatga aaacggagtt tactatgtga atgcggttga aagttttatg aatactgtga
     1321 gttcctctcc atttgttggt gcaaacttta ttgatattcc tgttatgcct aaacctttaa
     1381 ttgaagatga aggtcaattg gtatcagcag aaattaaaac atggtttacg gataaaaaaa
     1441 atgatcaaga gagggtagta agaaactcac ttgcttcaag ttatattaat ttattcccta
     1501 ctactattaa tttctatgat tattcaacca aagatacaga acaattattg aagttacgtg
     1561 aatcaaccta tttagtttct acgtttggtc aagaaattaa tggaatggat atgggggaaa
     1621 catacgaaat tagtaattat gagtttgtga ctgccgatcc tgaaagttta ataattacac
     1681 aaaaagatgg aaatgtcatt aatttatatt ataagaaact ttctagtgat gctgaatttt
     1741 atgaaccaga agtagaaaaa gaagaagtca actatggagg aacgattgat ctgac
//
LOCUS       DAXWLF010000253         1762 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXWLF010000253 DAXWLF010000000
VERSION     DAXWLF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.259"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..182)
                     /locus_tag="ACGE4U_002696"
     CDS             complement(<1..182)
                     /locus_tag="ACGE4U_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8394781.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACGE4U_002697"
     CDS             517..951
                     /locus_tag="ACGE4U_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8394782.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACGE4U_002698"
     CDS             974..1294
                     /locus_tag="ACGE4U_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394783.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACGE4U_002699"
     CDS             1435..1626
                     /locus_tag="ACGE4U_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394784.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..>1762)
                     /locus_tag="ACGE4U_002700"
     CDS             complement(1697..>1762)
                     /locus_tag="ACGE4U_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFF8394785.1"
                     /translation="ELTLSVIFLIIWIYFLYKNQN"
BASE COUNT          671 a          264 c          367 g          460 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tc
//
LOCUS       DAXWLF010000254         1746 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXWLF010000254 DAXWLF010000000
VERSION     DAXWLF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.260"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            136..309
                     /locus_tag="ACGE4U_002701"
     CDS             136..309
                     /locus_tag="ACGE4U_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394786.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACGE4U_002702"
     CDS             949..1422
                     /locus_tag="ACGE4U_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFF8394787.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ACGE4U_002703"
     CDS             1431..1658
                     /locus_tag="ACGE4U_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394788.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          587 a          272 c          260 g          627 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagat
//
LOCUS       DAXWLF010000255         1745 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXWLF010000255 DAXWLF010000000
VERSION     DAXWLF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.261"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..1576
                     /locus_tag="ACGE4U_002704"
     CDS             <1..1576
                     /locus_tag="ACGE4U_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFF8394789.1"
                     /translation="QINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDNAYT
                     KFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDP
                     DFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLAR
                     KRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEK
                     TKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSS
                     MESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIG
                     VYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDARE
                     EIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSN
                     EWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAW
                     RKMLDDELIPLENELR"
BASE COUNT          630 a          280 c          353 g          482 t
ORIGIN      
        1 acagatcaat cgtatgatcg atcaaatgga gtctgtcaaa aaagaaagtt atttggaccc
       61 tactgttttt gaagcagagg aacatatcca aaaagtcatt cataaaatgt tccgtccaga
      121 caatgcctat acaaaatttg attttcgaaa tggccgtcca acgaagtctg cgatcttaac
      181 cacgagttcc attgatatgg ccaaaaagta ttatcgggcc atcaaagaga tgacgaaaga
      241 atctgattgg ttaataaaag agtttcccaa tcagcctatc cgtgaaggac gaacgatgga
      301 ggatcctgat ttcccacgaa tcgctattac ctattcatta gaagaaaata gccgagattc
      361 aagtgcccaa caagaagaaa tgcaaaaaat tattgaggaa tataatcagt attatgatac
      421 tgcctggtca ctagcagaca ttgagcgtta taacggagac atcaataatc gtttggcacg
      481 aaaaagagcg gaattcaaac aatttgggaa acaaatcgat ttagtaatcg tagtggatcg
      541 tctattgaca ggttttgacg cccctacgat tcaaacccta tttgtggatc gtaatctaga
      601 gtatgcagga ttgatccaag ccttttcacg tacgaaccgt acgtatcctg aaaaaacaaa
      661 aggactaatt gttactttcc gaaagccagc gacaatggag aagaatgtcg aggatgcgac
      721 aaaactgtat tcagaagcta aagaagaatc gggtttgatt tatccaagtt accaagaatc
      781 caaaaaaaga ttcaagcaag catatggtaa gttaaaagag gtttcatcca tggaatccat
      841 tgatgaacat acgccacttg aaatccgtat cgagtacgtc aaagcatttc aagaattgaa
      901 tcgtgcgtat gaatcgttag tgacttatga tgattacaat gatgacatgg tgcaatcttc
      961 aactttgaac aatcaaattc aacttttgga agaacaaatt ggcgtgtacg agaccgtgaa
     1021 gggttcattg atggaggaag aaccagaaaa agagggagaa gatttttcaa cgatcgaatt
     1081 ttatagtgaa aataccacta agttgtatga tattgattct acttatattg atcaattact
     1141 aggaacttat gctgcgaata gttcagatgc acgagaagaa attgaaaaag ctttggctaa
     1201 attgaataaa acagaaggag tcaaagaagt ttaccgacaa attttaaatg catttgatgt
     1261 ggggacttta gatagtaatg aagatatctt tgtcattaaa cgtcgctatt ttacacaagc
     1321 aagtgataat ttgattcatg ccttttccaa tgagtggttt gtttctgaga aagaattaca
     1381 tgcttctaat ctgcaatata tgccaggtga agatccaatt cctaatatga aagcaattat
     1441 caacagtaag gattacgaag gatataaagc caaacatcca gaagcgaaac catttaaata
     1501 cccacaagaa atgaagcgag catggcgtaa aatgttagat gatgagttga ttccacttga
     1561 aaatgaattg aggtaaacga gaagagttaa gaaaattgta cgattcaaag atagtagtaa
     1621 agtggaagtg tagaaaatag ggatagatgt ctttttactt gttcacattt tataacggag
     1681 tatttaagaa agctattgaa taatgattct tccatctttt tcattcattt accggttctg
     1741 ttgca
//
LOCUS       DAXWLF010000256         1731 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXWLF010000256 DAXWLF010000000
VERSION     DAXWLF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.262"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..1084)
                     /locus_tag="ACGE4U_002705"
     CDS             complement(<1..1084)
                     /locus_tag="ACGE4U_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFF8394790.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQK"
     gene            complement(1194..>1731)
                     /locus_tag="ACGE4U_002706"
     CDS             complement(1194..>1731)
                     /locus_tag="ACGE4U_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFF8394791.1"
                     /translation="ATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISY
                     NYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVAS
                     WALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVAS
                     LSMIGVLIGRKVLKKYKK"
BASE COUNT          634 a          325 c          239 g          533 t
ORIGIN      
        1 ttttttgttc taaaactaag ttgaaatttt ctaaaacaca ctcttctttg tatgaaaagc
       61 tgacattttc aaatgcaatg tcacaatttt taatctgtgt ctcgttccca tattttagtt
      121 ggtcttgctt catttctaaa taaagggagt ctagtgtttc gactgcatat cttgcttcaa
      181 acaagtgcat acttacatac attattctca tgaaagaaac aaaaataatt ccacttaaaa
      241 aaaacaacat caacaagtca acagctaata tcttgggagt ggtaagagaa gttataaaaa
      301 aggttatagg aactatcaaa atgcaaacta tgccaaaaaa tagccattga aaaagaacaa
      361 aaggtgtttt cccactcaaa gaatacttat atgcaaactt agcataatcg ttaattgctt
      421 tattcaaggc tttaaatgat gttactttag caccaaatat ctttataact tgaattccgc
      481 ggatatattc taccgtttca gagcttaaca catctaacga ttcttgatat ttttgaataa
      541 aatttgtatt ctctgtcatt ttctttaaca agaatacacc aattactaac atggcgatta
      601 gaaccaatcc aattttaaag ttaatgaaaa agcctaaggc aactgataaa acaggtgtta
      661 ctatggcttg agcactatct ggaatcaagt gagcaattgc catatgtgtt ttagccgcgt
      721 tgtcatcaat tgtctttcga atatatcccg aagaatttaa atcaaaaaaa cgaaaacttg
      781 cttctgttaa accgctaatt cccttttttc tcaaattagt ctctaagcga aacccaagga
      841 tatgagaaaa taagccagaa aaaatataaa ataaagctcc taaagttaac caagcaacag
      901 tttgaaccgc ataactctta gctaaatatt catctcctac aataattaga gactgaagaa
      961 atttgtaaat actgtaatat ccatagactg ttaatacgac agaaaaatca gaaaataaag
     1021 tggctaacca tccgtagtat ttcattttag ggacataact aaataatttt ttgtatactt
     1081 tcattttttc ctcccccgca ttcaatttct tctcttattc gtttacaact gttaatgctt
     1141 tccgtaatag cgtaaattag agatgtacaa acagataaaa ggtaaactac gttctatttt
     1201 ttgtattttt ttaagacttt tcttccaatc aatacaccaa tcatagataa acttgctaca
     1261 actaacactg aaatgattcc ccatatacct accgcatagt ggatcattgc atccattgtt
     1321 gcagcagatt gtcctttatc tatccaatct tgacgaaaac gatctgtaaa gaaccatata
     1381 ggaatccact gtccggcagc actgcctaat tgcattagag cccaagacgc aacttgagga
     1441 attgctcgat catatccaaa tacagataga ataatatcag ctaagatcgc catactaatc
     1501 gaactgataa accaaggtaa gtacgccccg cccattaacg taaaaaataa caataaaaca
     1561 attgtgtaaa tagtaaaaat tccttttcta gggtagttat agctaatata aacaaatatg
     1621 attccagata ataatccaaa aatcccgggg gatatgctgt gaccaaacgc cccaccaata
     1681 ctactaacca ttattgaaaa aaagtaaatt actagggaaa taactgtagc t
//
LOCUS       DAXWLF010000257         1715 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXWLF010000257 DAXWLF010000000
VERSION     DAXWLF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.263"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            181..1659
                     /locus_tag="ACGE4U_002707"
     CDS             181..1659
                     /locus_tag="ACGE4U_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394792.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          623 a          236 c          303 g          553 t
ORIGIN      
        1 tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa aaaagataaa
       61 aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt ataggtgaaa
      121 agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga ggtgtaggat
      181 atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct agctagatat
      241 ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac attaggtttg
      301 tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac tgaattcaaa
      361 acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg tctgaatttg
      421 acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc tcgactgtta
      481 acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca ttttttaagc
      541 atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca gcatcaaata
      601 aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt attatttggg
      661 gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt catgaatgaa
      721 gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc tgcaacaaaa
      781 cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg gaatcaacca
      841 atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat gaagatcatc
      901 aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat tgatgagatt
      961 tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg cgcatttgga
     1021 cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc agtggaattg
     1081 attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata tatacgaaaa
     1141 gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga ctttgagatg
     1201 actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc aatattcagt
     1261 acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa agatgaacgt
     1321 ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg tccggtatca
     1381 tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc ttataccgat
     1441 tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga gagtcgaatc
     1501 acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa taaagaacag
     1561 ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa taccatcatc
     1621 agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat ggcagctttg
     1681 tctcacatat ttattagctg atttatgttt ttttc
//
LOCUS       DAXWLF010000258         1663 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXWLF010000258 DAXWLF010000000
VERSION     DAXWLF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.264"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..550
                     /locus_tag="ACGE4U_002708"
     CDS             <1..550
                     /locus_tag="ACGE4U_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8394793.1"
                     /translation="TFSSFLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGV
                     SVLVLVIVVLFFKELLITSFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTIL
                     IVAMLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAF
                     LFVISFLISPKQKFLRKKERSL"
     gene            547..1497
                     /locus_tag="ACGE4U_002709"
     CDS             547..1497
                     /locus_tag="ACGE4U_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFF8394794.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1512..>1663
                     /locus_tag="ACGE4U_002710"
     CDS             1512..>1663
                     /locus_tag="ACGE4U_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HFF8394795.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLR"
BASE COUNT          616 a          212 c          311 g          524 t
ORIGIN      
        1 tactttcagt tcttttttag cactaggagt tatccttata ggagttgcaa atagctcaac
       61 ggatttattt catattttat ttggaaatat tttagctgta caagatattg ataagtggat
      121 tacaattggc gtttcggttt tagtgttggt tatagtagtt ctatttttta aagaattatt
      181 aatcacttct tttgatccat taatggcaaa agcaataggg atgaatgtca gtttttatca
      241 ttatctattg atggttctat taactttagt agcagtaaca gctatgcaaa gtgtgggaac
      301 tatattaatt gttgcaatgt taattacacc agcagctaca gcatatttat atgcaaatag
      361 cttgaaaact atgatattta tttctgcaac agtcggagca agttcatcat tgcttggatt
      421 gtttattggt tatagtttta acatagcagc aggatcaagt attgttttaa cagcggcatt
      481 cttatttgta attagttttc taatctcacc aaaacaaaaa tttttaagaa agaaggagag
      541 atcgttatga tgagaaaatg gaaagcagta ctgggaagtc tggggatttt gattgctctt
      601 tttatattcg gggcatgttc aataaatagt aaagacaaag ataaagtggc ttcgaacgaa
      661 aaattaaaag tagtagttac taattcgatt ttagcagata ttaccgaaaa tatagcaaaa
      721 gataaaattg atttacacag tatcgtacct attgggaaag atccccacga atatgaacct
      781 ttgcctgaag atgttcaaaa aacttcaaaa gcagatttga ttttttataa cggtgttaac
      841 ttggaaactg gaggaaatgc ttggtttaca aaattagtta aaaatgcgaa caaagaggaa
      901 aacaaagact attttgcagc aagtgatggc atagatgtta tttacttaga gggtcagagt
      961 gagaaaggga aggaagatcc tcatgcttgg ttaaatttag aaaacggtat tatttacgct
     1021 aaaaatattg aaaaacagtt agcggaaaaa gatcctgata ataaaaaatt ctataaagaa
     1081 aatctagata agtatattga aaaattggat tctctagaca aagaagctaa atctaaattt
     1141 gcttcaattc cgaatgataa aaaaatgatt gttacaagtg aaggatgctt taaatatttc
     1201 tcgaaagcgt ataatgtgcc ttctgcttac atttgggaaa tcaacactga agaagaagga
     1261 acaccagatc aaataaaaca cttagttgaa aaattacgca caacaaaagt tccctcctta
     1321 ttcgtagaaa gcagtgtgga tgatagacca atgaaaacag tatcaaaaga taccaatatt
     1381 cctatctatt caacgatttt tactgattca attgcagaaa aaggacaaga tggtgatagt
     1441 tactatgcga tgatgaaatg gaacctggat aaaattgctg aaggcctttc gaaataattt
     1501 ggagggtagg tatggtacaa attaaaacga ctactatact aatttattta ctagcaattc
     1561 aggaattatc aaaaagaaaa aagaaattaa aaaataatga tttagcaaaa gttctaagtg
     1621 ttagtccagc atctgtaagt gagatgctgg ctaaacttag ata
//
LOCUS       DAXWLF010000259         1662 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXWLF010000259 DAXWLF010000000
VERSION     DAXWLF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.265"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..674)
                     /locus_tag="ACGE4U_002711"
     CDS             complement(<1..674)
                     /locus_tag="ACGE4U_002711"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFF8394796.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDR"
     gene            complement(646..1542)
                     /locus_tag="ACGE4U_002712"
     CDS             complement(646..1542)
                     /locus_tag="ACGE4U_002712"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8394797.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
BASE COUNT          575 a          287 c          206 g          594 t
ORIGIN      
        1 tttctatcaa aatatgcatc atacttcgta tttataaata ttgaactgat ataactttgt
       61 ccaatagaga ataggagcaa gctaaggatt gtaacatatc cagtaatctt tttattaatt
      121 tttacaataa aaaaagggag ataaaaatat gcaagattat ggaacagtcc agccactaag
      181 ataaaaaaaa tatacttaaa tatagctttg ctgcctcgca atttagttag atgataaaaa
      241 gctagtaata tgaatatcaa tgcgctaaac atacgtactt gtattgcttg tgaagtaaaa
      301 ccaaaaataa aaaacaagaa caaaactaaa aaaacattag ttgtgaataa tctgatgaca
      361 tacaataaag ctgagagtaa gacagtagaa ataatcaata aaaaaatctg atactgactg
      421 gaaataaagg tacatacgta cataacagct ctgaatcccg gttcaatact atctgaacta
      481 aagtccatac caaattgtgc tatcccatta tacaagacag aatatgcata ataatcagcg
      541 ttttgtgtgt taaatgccat gatgatccac attatcaaaa gtagcacccc agacaaaatc
      601 ttgttcgatg tactccattt tttttgtgaa acactctttt cttgattaat ctccaatgac
      661 gcctctgtat ccatctcgaa ccccccttat agccgctcta aatctactga gcttatcagt
      721 ctcaaaagct attgcaataa aaaatttcaa taatctttta ttcataaggc gggaaaattc
      781 cttatcactt ttatacattc ttttcaccaa tgttgaattt cttgcaataa aatactttcg
      841 tgttgaatta tgattgtcta ttttaatttt ttttgatcct atttttaaat tttctccatc
      901 tccaacgtga tgataaaaat agacttgatt tgttacataa atgttatagc cattatttgc
      961 aaccctttta caaaattcaa catcaatctg atcaataaaa aattcatcct tcatatttcc
     1021 aacatcgtca attaattcac ttttgaaaaa cattccagag gaatttgcaa atgctatttt
     1081 catggaacca ctagttgaag caaagtgttt ttttacgtat ctccctctct catttttgca
     1141 ggtaaaagag acagattcat cattgctatt ctcaaagtaa ttgggaacaa taatcccaat
     1201 gtccttatct ttcgcttcaa tattggtaaa ctcttctaat aattttaaag aaagattctt
     1261 ttgtatgatg gtatcttgat ctagtaaaag gatataatca catttctctt ttttggcaat
     1321 ttcaatacct atgttttgag ctgtcgcaag cccaatgttc tcattcattt tttttaaaat
     1381 taagttttcc atcatttcaa attgaaaaga gttaatatta tctgagccgt tatccacgat
     1441 aataagtttg ttctgtttct cttgtattaa cgtatccact aataacttta tgttattatc
     1501 tggattgtag gtaacaataa ttatcccaat tttatttttc atcgttcatc accttgtaca
     1561 cttcattcca tttcattttc aatacttgct cagagaatat agtggcgtaa atttttctaa
     1621 cattttcatt tatgttttta ttacactgat aaaatttcaa ca
//
LOCUS       DAXWLF010000260         1649 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXWLF010000260 DAXWLF010000000
VERSION     DAXWLF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.266"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..575)
                     /locus_tag="ACGE4U_002713"
     CDS             complement(<1..575)
                     /locus_tag="ACGE4U_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8394798.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKG"
     gene            complement(586..1254)
                     /locus_tag="ACGE4U_002714"
     CDS             complement(586..1254)
                     /locus_tag="ACGE4U_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFF8394799.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1384..>1649)
                     /locus_tag="ACGE4U_002715"
     CDS             complement(1384..>1649)
                     /locus_tag="ACGE4U_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8394800.1"
                     /translation="GTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIH
                     FYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          596 a          276 c          219 g          558 t
ORIGIN      
        1 aatcctttat tttttaaatc acaaagtaag tcagcaagtt tcttagtcgc tttgaagtcc
       61 aaatttgaag acggttcatc caatataatt aatttagagt ttagcgataa actacatcca
      121 atagaaattt tctgccgttc accacttgat agcgaaaaaa tgtttttatc taatatattt
      181 gaaagatcta ataattcagc taattcattg attcttttat ccatatgctc ttttgtaaat
      241 ccaaagtttt ccatcgaaaa tactaactca ctcgttgtat ttgttgtaaa gaattgtcct
      301 ctaggatctt gacttacgta gccaatattt tttgatatac tttcttgtgt taaggattgt
      361 agcgagttat tttcaaataa aatttcccca accaattttc cgtcataaag tcttggaata
      421 agtccattca ttaactttaa tagtgtggat ttcccactcc cagaagaacc agtaatcaat
      481 gtaagactgc ctttttttat tgaaaaatta aggtcaacaa gcgcttgttc cttaacatcc
      541 ttatatgaaa aagaaacatt gttaaattca aacaattctt tcacctcaaa tcaaaatact
      601 taagaaaaaa atagtaacgg aaaacaggat gatatagtct aataagctca tgtttgtttt
      661 atagtaagaa cttcttttca caggagattc tatccccttt gttattgcat tagcagtcat
      721 ttcttcagca atcatagttg cacgtataag aacgggaact aacagaaatt ccaaatattg
      781 cagaggctga aaaaaagaca agcgaatgtt tctcattttt aaagattctt taatcattct
      841 tatttcagaa cgataagtcg gaaagaatct gatcactact gtaatactta acattatttt
      901 tttcggtaaa tgtatttttt ctaaagagga taataattca ttgctagtat agacactaac
      961 caacaaccat gagatcatta cgaccggcaa cattcgaaat acaatatata aggatgtcgc
     1021 aaaaaatata gtaaaaaact ctatcttgat agcatcaata attttcatta ctaaaaacag
     1081 aactgaataa atcaagatga aataaataca tgtctttatt ttacgaaaca tcaatactaa
     1141 aactgtagca ataatgaaac accccacttg aaatatattt ccctttccta aaaaaataat
     1201 gaccggaaaa aaaatattta aagtaatcaa ggttaacgga ttgatttttg acactctttc
     1261 acctccaaaa aataagatcg cactgttatc ttaagctgat aaaaatgcac cgttattcag
     1321 ataacagtgc gatcttatca aaaagcataa ctgagaacaa ttctcaatgc aagctttttt
     1381 taattaactc agtaattcaa tccacccagg aacagaaaat tgatacaatc ttctggttaa
     1441 tagcatagtc ttttcttttg aatagctatg cttcaatata tcatagaatg tagcgtagaa
     1501 tgagtaaatg tagaagtgga tttcttcttc attaaagact aaattttgat tgatcattcc
     1561 gatagtcttt aacttatcta gacaacagac tgtctttttc gtcacaaatg atgtaatttt
     1621 ttcaacaata tcttgatatt tggttccag
//
LOCUS       DAXWLF010000261         1643 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXWLF010000261 DAXWLF010000000
VERSION     DAXWLF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.267"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            132..1274
                     /locus_tag="ACGE4U_002716"
     CDS             132..1274
                     /locus_tag="ACGE4U_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8394801.1"
                     /translation="MAKFEQYKKKNGDKAWKFQAYLGINPETGKPVKTTRRNFKTQRE
                     AKLALARLQSEYEDNLLTKEKPKTYKDVYDLWMTEYKRTVRGSTLLKTERIFKNHVLE
                     ELGDIYISEITPIKIQKLMDKWANKYDTAPKMMNYTGLVFKYAVRFGMIESNPTDAIR
                     KPKRRKKATVEEPFYDKKQLKLFLDELYNQPNLKIQAFFRLLAMTGMRKQEAGALEWR
                     DIDFKAKTVNIYKAVTRTANGLEIDTTKTVGSSRIISIDQGTLDKLLEWKKVALPPSD
                     EWLIFGHSSAKKPHDIMSLDTSRKWLLSIQDKMDKKQKKKLPRITVHGFRHTQASLLI
                     EMGASLKEVQFRLGHEDIQTTMNTYAHVSKLAKEQLADKFNKFIDF"
BASE COUNT          647 a          248 c          281 g          467 t
ORIGIN      
        1 atataaaaat tacaaaacat taatatgtta tgtgatgcac atccatttaa taacagcaga
       61 atattataat ggatgtgtaa aaatcactga gattttttag tcattattac cagaaaagtg
      121 agttgattca attggctaaa tttgaacaat ataagaaaaa gaacggggat aaagcttgga
      181 agttccaagc atatttagga atcaaccccg aaacaggaaa gcctgttaaa actactcggc
      241 gaaattttaa aactcaacgt gaagcaaaat tagcactcgc aagattgcaa agtgaatacg
      301 aggacaattt attaacaaaa gaaaaaccaa aaacatataa agacgtatat gatttatgga
      361 tgactgaata caaaagaaca gtacgaggat ctacattgtt aaaaacagaa agaattttta
      421 aaaatcatgt attagaagaa ctcggcgaca tatatatttc tgaaatcacg cctatcaaaa
      481 ttcaaaaatt aatggataaa tgggcaaata aatatgatac agctcctaaa atgatgaatt
      541 acacaggact agtttttaaa tacgccgttc gatttggaat gattgagtcc aatcctacag
      601 atgccatacg caaaccaaag agaaggaaaa aagcaactgt tgaagaacca ttctatgata
      661 aaaaacaatt gaaattgttt cttgatgaac tatataatca accaaaccta aagattcaag
      721 ctttttttag attactagct atgactggta tgcgaaaaca agaagcaggc gctcttgagt
      781 ggagagatat agatttcaag gctaaaacag tcaatatcta taaagccgtt actagaactg
      841 caaatggact agaaattgac acaactaaaa cggttgggtc tagccgaata atttcaatcg
      901 atcaaggtac tttagataag cttcttgaat ggaaaaaagt tgcccttcct ccatctgatg
      961 aatggctcat ttttggacat tcaagtgcaa aaaaaccaca tgatataatg agtcttgata
     1021 cctctcgaaa gtggcttttg agtatacaag acaaaatgga caagaagcaa aagaaaaaac
     1081 tacctagaat tactgtacat ggatttagac atactcaagc aagcttgttg atcgaaatgg
     1141 gagcatcgct taaagaagta cagtttcgtt taggtcacga ggatattcaa actactatga
     1201 acacgtacgc tcatgtatca aaacttgcta aagaacaatt agcagataag ttcaataaat
     1261 ttatagattt ctagctatgt ggcattcaaa atggcattca attaaatttg agcattgata
     1321 taatagtatt taagcaactc ccgccgtctc catatatgta tggttgagac agataaacat
     1381 gtatgaaaaa cgttgttaaa tcaacgtttt tttattttgg tcaaggagag aaaagtagag
     1441 aaatacaaaa ttttttgcat tgattttgca ttcaaaaaaa cattcaaaca tttagaaatc
     1501 cccaatacgt attcaggaaa atctatttta aggtttacaa aaagaacttt tgttcgtaca
     1561 ctctttttga ggtgaacttt tatgctgatg gaaggaaaaa cacaattatg gtttaagcat
     1621 gttccttcaa ataagttttt tta
//
LOCUS       DAXWLF010000262         1620 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXWLF010000262 DAXWLF010000000
VERSION     DAXWLF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.268"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..189
                     /locus_tag="ACGE4U_002717"
     CDS             <1..189
                     /locus_tag="ACGE4U_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HFF8394802.1"
                     /translation="TSGALISFSVENADEVKKFAQIAKENGGTFHEVDMGMPEEEMFS
                     LEVQDLDGNTLEPVWMKM"
     gene            426..968
                     /locus_tag="ACGE4U_002718"
     CDS             426..968
                     /locus_tag="ACGE4U_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFF8394803.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1051..1602)
                     /gene="lepB"
                     /locus_tag="ACGE4U_002719"
     CDS             complement(1051..1602)
                     /gene="lepB"
                     /locus_tag="ACGE4U_002719"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8394804.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
BASE COUNT          479 a          286 c          285 g          570 t
ORIGIN      
        1 acaagtggtg ctttgatttc gtttagtgtt gaaaatgcgg atgaagtaaa aaaatttgct
       61 caaattgcaa aagaaaatgg tggtactttc cacgaagtag atatgggaat gcctgaagaa
      121 gaaatgtttt cattagaagt ccaagatcta gacggaaata cattagaacc cgtatggatg
      181 aagatgtaag cagataacta atctgacgtt ttttataaaa aaaaaacaaa ggcggatgat
      241 acgataagta ttctccgcct ctattttttt tattcgtaca atctcttttt tacagtttat
      301 aaaagcagtc ttctcaccct ttttttaatt gttagatgcc agtgtatgta aatcaccaat
      361 gtgtcatgac acattggtga ttttatggta tccttttatt attaactgac aaaggagaga
      421 atattttgaa cactttgatt gcaaaagacg tttttagatt gcccgaaatg aatgcaagat
      481 tgttgtcgac ctctgctctt ttattagccc ttgatgtcat cttatacaag atagcgattg
      541 gtcctgctta ttttcaaatc acattcggat ttttaagtat ggcgcttatg ggttatctat
      601 atggaccgat ctgggctggt ttgcttgaaa tgttgtcgaa cactttaaat ttcacgatat
      661 ttggttcagg aacgtttcaa gctgcttttc ttatcacggc tttccttggt ggatttatta
      721 atggcttatt tttatatcat aaaaagataa acctgccttc cgtcatcgct gcccaattat
      781 tgattatgat cccagtcagc ctttttatca attcttggct gatgtctgtt ttgtttggtg
      841 cagatttcaa agtaattttt ctaggtagat tcatcaggaa tatgatccaa atccctattc
      901 aaataatggt tgtttattca tttatcaaaa ctttagaggc tagaaaagtg tttaaacgag
      961 ctgtttgatt ttacgtacta aggtagtaaa ctgattttgg attaaacaat aaagcgtgca
     1021 gtctcaacag actgcacgct ttattgtttc tcactggatc cattttattt catcaagtgg
     1081 ataatagaca aatcttgctt tacctagaat atcagtttta ttgatcgtgc caaacgaacg
     1141 actatctttt gaaactcttc gattatctcc aagtacaaaa taattatctt ccggaagctt
     1201 ggattgtccc gttagctctt gtagtgtaaa gtccgtcgta taggatgcat ttgcatgatc
     1261 tgatttcagg tttttagtta gataaggttc cttaactact ttcccattga tcttcaattg
     1321 atcattttgg tagctgacat tttctcctgg aagaccgatt actcgtttga tataaatcgt
     1381 gccatctgct aattgaaaaa caacaatatc aaatcgtctg atctccgaaa atttttccat
     1441 cacaaccata tcaccttgct tcaacacgtt ttccattgaa ttgccttcca ctggtaccgg
     1501 gatgaataaa aatccccgca acatgaacgc aaggaataca gaaacaagta agtatttgaa
     1561 tacaagccag aagcgattaa tatagcgttg tttttttgtc aatctctatc aatccatccc
//
LOCUS       DAXWLF010000263         1566 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXWLF010000263 DAXWLF010000000
VERSION     DAXWLF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.269"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            243..1412
                     /locus_tag="ACGE4U_002720"
     CDS             243..1412
                     /locus_tag="ACGE4U_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFF8394805.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          572 a          224 c          250 g          520 t
ORIGIN      
        1 attcttttat tactatgttt gggttttata gcaaagaaag tttaataaga gtttggacct
       61 agcaaacaat attaatgagg tcattaaatc ttattatctt tagtgatata acgaaatatc
      121 tacacagaca aaatgatagc gtaaaatatt ttgtgtaaat agatatttag ggtagaaaaa
      181 aagaaagtcc attctgtaaa attaaagtta ccacaaccga aattttatag gaggactttc
      241 tcatgcccaa ttttactaca gaaattatgg aaacactaat caataaaggt gatttagatg
      301 aattatttcg atgtcattta gaattagctg ttaattcatt attgcaggct gaattaacag
      361 cttttcttga ttatgaaaaa tatgatcgtg ctgggttcaa ttcaggtaat tcccgtaatg
      421 ggaattattc acgttcattc agaacagagt atggagaatt aaatttggta attcctagag
      481 atagaaatgg taagttttct caacaaactt tacctgctta taaaagaacc aatgactctt
      541 tagaaaccac tattattcag ctatttcaaa aaggaattac tatgtctgaa atctctgaat
      601 tgattgaaaa aatgtacggg catcactata cgccacaaac catttcaaat atgactaaaa
      661 tagtttctga agatattatt gcctttaaag aaaggtcctt agaatccaga tactctgtca
      721 tttttatgga tgctactcac attcctttaa aaagacaaac tgtttcaaaa gaagcagttt
      781 atattgtaat tggcattcga ttagatggaa ccaaagaggt tcttgggttt agtcttgctc
      841 caactgaatc tgcttatgtt tggaaagaga ttcttcaaga cctaaaagac cgtggtttaa
      901 aagaggtttt attagtcgta actgatggtt taagtggcat taacgatagt attcatagtg
      961 tttatccaaa tgctcaattt caacaatgtt gtgtccatat atctagaaat atcgctcata
     1021 aggttcgtgt tagtgatcga caagaaatct gtagcgattt caaattagtt tatcaagcaa
     1081 gttcaaaaga agaagcaaat aatcaaataa ggtttatgat agataaatgg aaaaagcagt
     1141 atccacgagt ggttaaattg ctcatgaatc ctgctatatt aaccttttat aatttccctc
     1201 cgtcaatcag aagaactatt tattcaacta atttgattga aggttttaac aaacaattaa
     1261 agaaatatac caaaagaaaa gagcaatttc ctaatgaaga atctctggag agatttctag
     1321 tatctcaatt caacaactat aatcaaaagt ttctatgccg agttcataaa ggttttaaag
     1381 aaatacatga tacattagaa tcaatgtttt aacttgatca acagaatggg cttttccatt
     1441 tacacaaaat tattgacgct cccgacaaaa ttatgatctt gcgtaaagtt ataaaatatt
     1501 aataaaaata ttttattaat gaaaagcttg gtagcacggt tattcatatg ctaatttatt
     1561 tcactt
//
LOCUS       DAXWLF010000264         1543 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXWLF010000264 DAXWLF010000000
VERSION     DAXWLF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.270"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..499)
                     /locus_tag="ACGE4U_002721"
     CDS             complement(<1..499)
                     /locus_tag="ACGE4U_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFF8394806.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKL"
     gene            833..1066
                     /locus_tag="ACGE4U_002722"
     CDS             833..1066
                     /locus_tag="ACGE4U_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFF8394807.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            1063..1470
                     /locus_tag="ACGE4U_002723"
     CDS             1063..1470
                     /locus_tag="ACGE4U_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFF8394808.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          535 a          201 c          279 g          528 t
ORIGIN      
        1 ataatttttt ctcaatggca ttggcacgtg cagctaacgt agttttatct gtgatggttg
       61 ctacttgatc cataggatta ttgtctaagt atggttttcc tagaggatta ttggaacttt
      121 tcgaaagata tttaaataga acctttaatg ccgtgatatt tctattgata gtagaagtag
      181 atagacctaa gttattatta tctttgagct tagtttttgt tgtctctttt tttacatttt
      241 cttttgtgcg acgtttcaaa tgagatttgt acaagtttag gtcataggca gttaactgtc
      301 ttatcgaggt gatagaagta tcaggaacga tagactcact cagaagccat tcataaaaga
      361 gcttataatt ttttagatat tctaaagtgg ttcgaggtga ttgatcttta tcctcacgat
      421 cttgaacaaa atcttttata taatcgggca tatgaagtat gattgtatct attttttgat
      481 atatttctgt atttttcatg tcattcatcc tttcaataag taggggagaa gagtatctct
      541 ataattggac tataccaatt atagaatgtt ttgtcaattg gtatgctcta aaatttatta
      601 atttgtttca aaaaaagcat ataaacaggt atacgtttaa taatttaaaa taaatggtat
      661 acataaatag ggaaggaaat agaagggagc aatctaaatt ttttgaattt tcttaattaa
      721 ttatatagaa agaacaatct ttctatagag ttagacatga gagggactat gcttgattta
      781 aaatcaacaa gcgtatatac tgtatataca aagaaaaagg agggagaaga ctatggcaat
      841 taaagaaaga aaatttaggg aagtggggaa ctcagtagta ttaactattt caaaggagtt
      901 tcttaaagaa aatgggttaa atcccggaga tacggttttt ttagatgaag aaaaacttcg
      961 agcggcaatt acaaaaaaac aagccttatc tgatgaaatt gatttagcaa tatctcaagc
     1021 attaactgaa tatgatacta cttttagaga gcttgtcgat aaatgagtat tcattaccta
     1081 gaagccaaag atattataaa aatgaatgta atccaaataa aaaaatactc tcctaatgaa
     1141 ccccttggtg taaaggatgc gaatgcttta gaaatgtgtg tagggcaact tcgagcctca
     1201 gcttttggtg aagaggttta tccttcgatt tatgaaaaag cagctatcct acttatacaa
     1261 ttgataaaga aacatccttt tcacaatgca aataaacgta ctgctttctt agctactttt
     1321 gtctttttga aaatcaatgg acagctttta gctattgatc aaaaagatgc tgtaaattta
     1381 gcggtatata ttgctacgta tgataaggat ttcgatcaac taaaatatga agtgatcaac
     1441 actataaaga cacatacgca gcccatttaa ggagttactg tatgtgtctt tatagggtta
     1501 ttagctgcta tagcgaaata attgaagtaa attacaaata gct
//
LOCUS       DAXWLF010000265         1539 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXWLF010000265 DAXWLF010000000
VERSION     DAXWLF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.271"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(52..1518)
                     /locus_tag="ACGE4U_002724"
     CDS             complement(52..1518)
                     /locus_tag="ACGE4U_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFF8394809.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          502 a          326 c          289 g          422 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattcttta
//
LOCUS       DAXWLF010000266         1531 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXWLF010000266 DAXWLF010000000
VERSION     DAXWLF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.272"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..1342
                     /locus_tag="ACGE4U_002725"
     rRNA            <1..1342
                     /locus_tag="ACGE4U_002725"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1437..>1531
                     /gene="rrf"
                     /locus_tag="ACGE4U_002726"
     rRNA            1437..>1531
                     /gene="rrf"
                     /locus_tag="ACGE4U_002726"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          428 a          329 c          449 g          325 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg t
//
LOCUS       DAXWLF010000267         1521 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXWLF010000267 DAXWLF010000000
VERSION     DAXWLF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.273"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..963
                     /locus_tag="ACGE4U_002727"
     CDS             <1..963
                     /locus_tag="ACGE4U_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFF8394810.1"
                     /translation="NEKYMDLSNDAKIAYMLLKDRFDYSVKNNWVDSDDNIFFIFTVE
                     ELMKLLQCREGKVSKIKKELEKAGLLKQKRGRVNKRDGKIESMPNLLYLGKPEVTNQD
                     VFKIMEEEDNTDTTVIAKIANTAKPREINDTTVIAKIANTAKSSHIKDSTVIAKIADN
                     LFYSNSIDTNRHIIDTETDQLQNQVLLDNFVDIMKEDSINTFVPENVLNLIKTFSSTY
                     EDAQKTVQTIHNAKKKAEELEQTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVN
                     NLQNLIFVYVKNWFVEKAIAAKLANEQRETFNELPTVSTENWLE"
BASE COUNT          583 a          225 c          261 g          452 t
ORIGIN      
        1 aacgaaaaat acatggatct atcaaatgat gcaaaaattg cttatatgct acttaaagat
       61 cgatttgatt attcagtcaa aaataactgg gtagattctg atgataatat atttttcata
      121 ttcacagttg aagaattaat gaaattgtta caatgccgcg aaggtaaagt atctaaaatc
      181 aaaaaggaat tagaaaaagc aggtcttctg aaacagaaga gaggaagagt aaataaacgt
      241 gacggtaaga ttgaatccat gcctaattta ttgtacttag gcaagccaga agtaacgaat
      301 caagacgtct ttaaaatcat ggaagaggaa gacaatactg ataccaccgt aattgcgaaa
      361 atcgcaaata cggcaaaacc gcgagaaatc aatgatacca ccgtaattgc gaaaatcgca
      421 aatacggcaa aatccagtca tatcaaggac tcaaccgtaa ttgcgaaaat cgcagacaat
      481 ctattctatt ctaattctat agacactaat agacacataa tagacactga aactgatcag
      541 ttacaaaatc aagttttact agataacttc gtcgacatca tgaaagagga cagtataaat
      601 acatttgtac cagaaaatgt attaaacttg ataaaaacat tttctagtac ttacgaagac
      661 gctcaaaaaa ccgtccaaac tattcataat gcaaagaaaa aagctgaaga attagagcaa
      721 acagttgttg tttatgaaga actggaatct tatggaattg atgctgacaa aggattatat
      781 atgactttgc tgaaagctta tcaaaaacaa aaaactgaaa aagtaaacaa tttgcagaat
      841 ctaattttcg tttatgtaaa aaattggttt gtcgaaaaag ctattgcggc aaagcttgca
      901 aacgaacaaa gagaaacatt taacgaatta ccaacagtta gtacggaaaa ttggctagag
      961 tagttgataa aaagcgatca ctataaaaat gatcaatttt aaattagaac ttttttatga
     1021 ttttaatttt attacaaatg ttacaaataa atagctatta ttccggagtt tttagattaa
     1081 aagtttatga aaacaatcac aaacgtaggt tttaaagtca taatcgttag atattttagt
     1141 tatttttcat tcaaaaaaat agggaactag tgtttgcata tgtgcttttt tgagttataa
     1201 taattcatga agagaggtaa ttcacttatt tccccaagct gaaaagcctg atattacttc
     1261 ttttcaggca gtgctgctaa tattcaatta cacttttttg tatattttta gttgttttaa
     1321 atttaattgt ctgatatgct tgtgatgaat tgattttcgt tctgcacttt tggacgataa
     1381 attgtgataa ttgaaaatac aaaaaaagaa aggcccctgt aaggctcggg agactggtaa
     1441 tctccgtcag tgtcctacag gaaagcatta agatgacgcc ttaatgcgtg cccttcagtg
     1501 ttacggtatt acccgcaacg g
//
LOCUS       DAXWLF010000268         1516 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXWLF010000268 DAXWLF010000000
VERSION     DAXWLF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.274"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            135..398
                     /locus_tag="ACGE4U_002728"
     CDS             135..398
                     /locus_tag="ACGE4U_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394811.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            422..610
                     /locus_tag="ACGE4U_002729"
     CDS             422..610
                     /locus_tag="ACGE4U_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394812.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(693..878)
                     /locus_tag="ACGE4U_002730"
     CDS             complement(693..878)
                     /locus_tag="ACGE4U_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394813.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1093..1464
                     /locus_tag="ACGE4U_002731"
     CDS             1093..1464
                     /locus_tag="ACGE4U_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8394814.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT          542 a          205 c          298 g          471 t
ORIGIN      
        1 tccatgatac aaaataacac tttgtgcgaa atggcgcaag cagttttgtg gatttatccg
       61 gtaaaatctg tatcgatttt gaaattagag gagaaacttg ctataatcaa agtgaaagaa
      121 ggagtgggat tttcatggat catgtagtgt tagaaatcga tttgcatttg atcaataata
      181 ttcgggtgat ttactatgtt gtaagtaaca gtgtcgaaca acgtgtactg acgaataaaa
      241 taaatggtat tttagcaaag aaagacgttc accgattcaa taatggtgaa ggaagttatt
      301 atagtatccc agtagaaaaa attatttata cgacagtcaa agtcagagaa gatctagata
      361 ccaaaaaagc atatgaacct atttttacaa cttattgatt gacagcagat ggaggaaaaa
      421 aatgaatcca tatgaacaac ttattcaaga tttgatcgat ggaaaaatcg aaaagattga
      481 tgtaaaaaga gaagaattaa tggcttttcg agaagcgtgg ctgaagttgg agaatcgtaa
      541 atatgtggtg ggaaaagcct cactgaacgg caatgtgact tatcattacg atccgacacg
      601 tttattttag tatgaagtat tcagaaaaga accaaaggag gacgatgcag cagcattgtc
      661 ctcctttgat tttacaaaag tgaaagtttt acttatttga atagattttt aaatttgttt
      721 ttgacactgt cgatatttac atcttttaaa agattttggg ctttctcttt ataaggtcct
      781 aattgttctt tatgttcttc gataaatcgt tttgcgccat cgaaattgcc gttcttaatc
      841 atttccttga ctttttgaaa taattcttct tgattcattt tgaacacatc ctttgctatc
      901 aacatgattt tttacatgga caaaacgaga aaaactgatt tatccacacg tcaataatat
      961 caaataacga tcatttttac taaaaaaaca ctcgattctc gcttctaaag taggagaaaa
     1021 atctccaact tgggacggat ggaatttctt taactgaccc atatactgaa tatataacaa
     1081 ggaggtagaa agatgaaagt gattaaatat ttgattggac tggcggcgat tagtggtatt
     1141 ttgctatttg gactgaaata ttatactgaa ggaagttatg gagaaatccc tggtatactt
     1201 gatcagctaa atccattagt tgaaaaagga gaggtctatg taaagacaca aaaaccagag
     1261 gaagtaaatg aatatggtac agcaaggtat gtgcagaaag ctgctgatgc caatgggaaa
     1321 gaacgaacgg ttgaatttaa tggtctttct gtgttaaaag aaaatcatta tttgaaaatt
     1381 agcaataaag gagcacacat agaaacgtat gaggaagttc ctagagaaaa agttccggag
     1441 aaagcattag caattattgg gtaaatcagt ttttgtatta agaatacgcc cgattttatt
     1501 attcataatt taatta
//
LOCUS       DAXWLF010000269         1513 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXWLF010000269 DAXWLF010000000
VERSION     DAXWLF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.275"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(192..1484)
                     /locus_tag="ACGE4U_002732"
     CDS             complement(192..1484)
                     /locus_tag="ACGE4U_002732"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFF8394815.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          412 a          341 c          271 g          489 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagagac agcctatcgg caataaggct aaacgtctct ttcactacct cttcaagtta
      121 ttattatcgt atagataaac acttcgactc gctctggtaa gaacgtcctg attcagtcgt
      181 cgtttttatc cttagatttg cgcccagacg ctttctaaga tgtttgtttg tgttctatca
      241 ggtccgacag aaaatgttga gatacgtaca ccaacaagct ctgagatacg acgtacatag
      301 ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc ctgtaatgtc ttctgaccag
      361 cctggcaatt cttcatagat tggtttgcag cggttcaact ctttcaagct tgctggatag
      421 tggtagatta gttcgccatc taattcataa gccgtacaaa tttttactgt ttccaatcca
      481 cttaatacat cgatcgagtt taaagatagg ttggtaatac ctgaaactcg tttagaatgg
      541 cgcataacta cagaatcaaa ccagcctaca cgacgcggac gaccagttgt cgtaccgtat
      601 tctcgtccta cttcgcggat ttgttctcct gtttcatcaa ataattcagt tggaaatggt
      661 ccatcaccga cacgtgacgt ataggctttg cagacgccga caactttatt gatttttgaa
      721 ggccctacac cgcttccgat tgttacccca ccggctactg ggttagaaga agtaacaaaa
      781 ggataagttc cctggtcgat atccagcatt acaccttgtg cgccttcaaa caacacgcgt
      841 ttgcctgcat ccaaagcatc attcaagata acggaagtat ctgtgacgta ttgtttgatt
      901 tgttgtccgt attcataata ttcttcaaaa atatcgtcaa aatcaagtgg ttctgaatca
      961 aacattttta caaattgacg gtttttgtca tctagattca aacgaagacg ttcttcaaaa
     1021 atttctttat ctaataagtc tgcaatacgg atacctacac gagctgcttt atccatatag
     1081 gcaggtccga ttcctttaat cgttgtacca atcttatttt cgcccttagc atcttcttgt
     1141 aattggtcca atttgatatg ataaggcaag attacgtgcg cgcggtcaga aatacgcaaa
     1201 ttacttgttt ctacaccttt ttcatgtaaa taagctaatt ctttaaccaa tgatttagga
     1261 ttgactacaa cgccgtttcc gatcacactg atcttgtctt tataaaaaat cccagaagga
     1321 atcaaatgca gtttataagt cacaccgtta aacttaattg tgtgccctgc attgtcgcca
     1381 ccttggtacc gtgcaatcac ttcagcattt tcactgagaa agtctgtgat ttttcctttc
     1441 ccttcatcgc cccattgcgt acctactact actacagatg acatgtgaac acctcgttct
     1501 atattattta atc
//
LOCUS       DAXWLF010000270         1502 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXWLF010000270 DAXWLF010000000
VERSION     DAXWLF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.276"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(39..575)
                     /locus_tag="ACGE4U_002733"
     CDS             complement(39..575)
                     /locus_tag="ACGE4U_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFF8394816.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     regulatory      complement(613..711)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(898..1320)
                     /gene="mgsA"
                     /locus_tag="ACGE4U_002734"
     CDS             complement(898..1320)
                     /gene="mgsA"
                     /locus_tag="ACGE4U_002734"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFF8394817.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT          462 a          310 c          264 g          466 t
ORIGIN      
        1 aacaagcagt cttctgggat gggaagactg cttgtttttt atttagcgtg gatcgctaaa
       61 cttcttaaaa tttttttgta aggaatacta cgtcctacgt aataaatggc aattgattga
      121 atcggtaacc atattactgt ttttaacagg cggggcgccc aaacgaccca gctgaacaat
      181 ggcacatggt acatcaacgc aagccataat ggtgtcaaaa ataagttgat gatcagcgtg
      241 acactaagcg tagccagaat ggcatttttc caagtgatct cttttcgata gaaaaagaat
      301 ccgtagattg ctccttcaat aaatgcattc aatgtaaatc caataaagaa tgcagatttc
      361 gagaagaggg ccattcctac taaatcagct aaaactgcgc ctatccctga ccaaaaagga
      421 ccaaaaagaa tgcccatgat cacttgtgga ataaatgtga cactgatttt aataaatggg
      481 gtctcgattg ctaccagtcg ggacaaagtg accatcaatg caatcaataa gcccataata
      541 gcaatcatac gtgcgtctaa ccggtgtttc ttcattttta gcatctcctt taagagctgc
      601 tacatcgtat catgcagcga atgcgaaaat aaaaccgcga acattacgtc cttcgtccaa
      661 gaggcaacat cccatcctct tagcacttaa cgctttattt tctactctgt attatttttg
      721 ggatcttttc ccacagaaac tatagcatat tttcacattt actgcaaata cgaaaaacta
      781 atattattgt ttatattatc caatacgttc gtattatatc gcatattatc agtttgttta
      841 gcttttatag ctacatatag gcgaacatta aacgttattt ttccatacgc ttatccatca
      901 aaatgaaagt ggatgcttgt ctatttcatg gacgattctt cgaaaatcta ggaagccggc
      961 ttccagtcct cttagtagca cctcagctgt tccgatattc gtagctaacg gaatctcata
     1021 gacatcagat aaacggataa gtgctgttac atctggttca tggggctgtg cagccagcgg
     1081 gtctcgaaga aaaatcacca aatctaactg atcctccgaa atcatggctc caatttgctg
     1141 atccccgcct aacggtccgg atttaaaccg atggatcggc aagccggttg cttccgtgat
     1201 cctctgccca gtcgtcccgg tagcaaaaag ttcatgtttt tccaatattg ttttatacgc
     1261 tgttgctaat ttcaccatca taggtttttt tcggtcatga gcgatcaaag cgattttcat
     1321 aagagacact ccttttgata ttgttaacat acttatttac tccagtgtat cagtttttat
     1381 ttcttatgga tatttattaa agcttaaaac tagcacattg acaaaaaaaa gaaaaaattc
     1441 ttgttcaaca tgacattgaa gttagttaac gctttcattt atactttaaa tagttaataa
     1501 aa
//
LOCUS       DAXWLF010000271         1501 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXWLF010000271 DAXWLF010000000
VERSION     DAXWLF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.277"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(61..1191)
                     /locus_tag="ACGE4U_002735"
     CDS             complement(61..1191)
                     /locus_tag="ACGE4U_002735"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFF8394818.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1251..1478)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          415 a          310 c          236 g          540 t
ORIGIN      
        1 ttctccactc catgaaaagc cgactttttg ggacgctttt ttttgccaag cctcacattt
       61 ttatagccaa acttcttttg cgacttgctg caataattgg attttcttcc attgttcttc
      121 ttctgttaaa tggtttcctt cttctgttga agcaaaccca cattgaggac tgatacttag
      181 attttccaat ggtacaaatt gttgtgcttc ttttattcgt tcttttattt tctccgtatc
      241 ttccaaatct ggaaacttag aagtaactaa tcctaaaaca actttacttt ctttgttact
      301 ccaatgtctt aaaggagtaa aatctcctgc tcggtcagaa tcgtattcca aaaagaaacc
      361 atcataattt gttgaaaaca actcttctgc aatgggttca tatccaccgg aaaacagcca
      421 agaagaagcg tgattcccgc ggcagatatg tgttgtgatc gtcaaatctt ctggtttgtc
      481 ttccagtgcg ttattgataa tctctgtaca aatatgggcc atttctttcg gatcaattcc
      541 tttttgaaga agtgcttctc ttttagtttg atcagctaga aacgcccaat ttgtatcatc
      601 gaattgcagg taacggcaac ctttttcata aaaagcatga atcgctgctt tgtaagtaga
      661 ggacaagtcc tcataaaaat cagatagatc aggataaatc tccttgattt ttgaagaacc
      721 atcattagat aggatctctt gtcgtaaaat catcgtggga ctagggatcg ttgcttttgc
      781 aacaaaagta tcatctgttt ccactacttc tttcagaaaa gcaaaatctt ttaaaaatgg
      841 gtgatttgga ttgaaagata atttatctac tacccgaata ttataagctg gtgctgcttt
      901 tccgtggaat gtttgattat atccagtttc tggaacatag ccttcgatac catttagttc
      961 ttctaaaaaa tcaagatgcc aaaatccacg ccgaaattca ccatctgtaa ttcctttgat
     1021 gccgactgct ttttggtctt ccactaattt ttttatttcc tggtcttcga ttgcacgaag
     1081 ttcttctttt gaaagtaagc cttcttgaac tttttttctt gcttctttga tcgattgcgg
     1141 gcgtaagaaa cttccgacat gatctactgt aaatggtcta tttttgctca tttcactcac
     1201 tcttttcttt gttttttgta aaaaaaaatc cttttactag caaatactag taaaaggacg
     1261 attatactcg tggtaccacc ttttttcaga aaaaacattc tttctctcag cctgacaata
     1321 aaatcagtgg tcttggtaac gaaagactgt tcgtttttgc ctacctttcg acaaaaaatc
     1381 ctctaaggcc attttcgaca gtcaacttct agctttttac accaaccaaa gcctctcttg
     1441 aaccattttc tgtctacttt ccttctcatc ggatactatt agaataaaat tagaatagca
     1501 t
//
LOCUS       DAXWLF010000272         1456 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXWLF010000272 DAXWLF010000000
VERSION     DAXWLF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.278"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(310..>1456)
                     /locus_tag="ACGE4U_002736"
     CDS             complement(310..>1456)
                     /locus_tag="ACGE4U_002736"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFF8394819.1"
                     /translation="DKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVL
                     SAKDILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLL
                     YHAEHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVY
                     ECDLACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVY
                     IHDIDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSN
                     DLAQMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPI
                     EDVCFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT          413 a          308 c          223 g          512 t
ORIGIN      
        1 cgattgtcaa ctctttttta aaagtttttt tcgatttcgt ttttcgaaat ctttcagcaa
       61 ctcatttatc ataacttatc gtttgttatt tgtcaagaac ttttttgaag tttttttcga
      121 tagaatcaag cttcgcaagt ggttcttttt aacgacttag ttatcttatc acgtctaaaa
      181 acatgtgtca actctttttt ggaattttca taaaatgttt caatgaagcg cgacaacgtt
      241 gtttaatata tcattaaaaa aaataaaggt caagctaaaa tattatttaa gcttgacctt
      301 attataattt caacgattgt caaaagtatt gtttgcagct gtgttgccag tttctataga
      361 taaatcacag cttttgaaac ataagttttg tactgcttcg attcgcccga attcttttgc
      421 agttatttgg caatgagaaa agcagacatc ttctataggc ttttcaggaa atgctactaa
      481 atcaaaagct cgggaaggaa gtgagtaacc tggagaaagg catgcctgta cgtttttgat
      541 gtgtatgtct ttgactttcg tcatttgctt tgattcaggt atttcttcag ccactgcttg
      601 ccaactttct ggcatttgag caaggtcatt gctatttttt ctattgtatt cattatgcca
      661 gtccattatc caagaaaaag gaaattgtac atcaatcata ttcagatttt ctaccctgat
      721 attttcgatc actccccctc tttcttttga agatttcatt cgaataccac agtctgactg
      781 aaaaaaatcg atatcgtgaa tatatacgtc actaactcca gcactaactt cgctaccaat
      841 agttacaccg tatcctgaac ggattttaca tctagcaacc tcgattcttg atgattttcg
      901 attgactctt gcaccattgc catctctgcc agacttaata gcaatacaat catcaccaca
      961 ggccaaatca cattcataga cacgcacatt tgttgaagaa tcaatatcaa tcccatctgt
     1021 acttggtcca tcattgttgc ggatgattac tttttctact aatacatcgt tagaataagt
     1081 gatttgcaag ttccaaaaac ctgatttctg aaaaatcaaa tcacgaacaa caacatgttc
     1141 tgcatgatac agtaaacaag cgcgtggacg tttaatagca taatccgcaa tccaacgaag
     1201 tcccttttga tcgtattcag ctcttgtccc gcttttttga tcttttcccc aatacagctc
     1261 ccaccaatgg tctccttgac cgtcgattat tccttttcct tctataagaa tatctttcgc
     1321 cgagagaaca ttcaaaatcg ctgctggcca tttcatttct acgccggcta ctcgtgtatc
     1381 gatttctgga aatgcttcga tatctgttct ccccttgagg actgcacctt cttcaaagat
     1441 aagatgactc ttatct
//
LOCUS       DAXWLF010000273         1451 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXWLF010000273 DAXWLF010000000
VERSION     DAXWLF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.279"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(100..1119)
                     /locus_tag="ACGE4U_002737"
     CDS             complement(100..1119)
                     /locus_tag="ACGE4U_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8394820.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1130..1336)
                     /locus_tag="ACGE4U_002738"
     CDS             complement(1130..1336)
                     /locus_tag="ACGE4U_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8394821.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          402 a          303 c          246 g          500 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgatc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcata tctatcctcc tatttatctg
     1141 atgaaatttt cttatacgtt cgatttgata cacctaaaat actccctaga aatgctccaa
     1201 aacctgtaat aatcatcacc gttatatctg tgtaccgcca gttaacacct tttccgacaa
     1261 gaccgacaaa agtcgctaaa gcggggataa ctactatagc gatccatttc aaaatatcaa
     1321 aggttctatt gttcatacta tccacctccc ttcattggtt gggctacttt ctttttcaat
     1381 cgcttgtttt cttctatcaa tatttctctt tctgaaagca aggcatcaat ttgcccgtcc
     1441 agttgttcaa t
//
LOCUS       DAXWLF010000274         1448 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXWLF010000274 DAXWLF010000000
VERSION     DAXWLF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.280"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            162..776
                     /locus_tag="ACGE4U_002739"
     CDS             162..776
                     /locus_tag="ACGE4U_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8394822.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(800..1150)
                     /locus_tag="ACGE4U_002740"
     CDS             complement(800..1150)
                     /locus_tag="ACGE4U_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8394823.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1144..1407)
                     /locus_tag="ACGE4U_002741"
     CDS             complement(1144..1407)
                     /locus_tag="ACGE4U_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFF8394824.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          460 a          273 c          233 g          482 t
ORIGIN      
        1 gccatttaaa aagaaaagtg acacatacta tttcctccat cattagtttt tgtctatata
       61 aggtataccc taactagaca cgaccgcgac attgaaaaat ctggttttct cgtgtatgat
      121 tagggtgttt tttaaatagt tgcagaaagt aggtaaaaat tttgagagtt gcatacatac
      181 gtgtctcatc catcgatcaa aatgaacaac ggcagattga agaaatgaaa aaattcggtg
      241 cagagagaat tttcattgag aaacaatctg gcgcaacgat tactcatcga cctgtttttc
      301 aagaagcact ggatttcgtc cgagatcaag atatttttat tgtggaagct attgatcgac
      361 ttggaagaaa ttacgacgag atcatcgctt cagtcaatta cttgaagaag aaaaatgtgc
      421 agctgatcat cacaagtcta ccgatcatgg ccgaagcaat tggcaatcct ctcctggata
      481 aattcattaa agatttgatc attcaaattt tggccatgat cgctgaacaa gaacgtaccg
      541 aatctaaacg acgtcaggca caaggcatta agattgcaaa agcgaacggt gtttataaag
      601 gacgtcctaa gctatacagt gccaatgcaa aagatcccca acgtcgtctt gtttataaaa
      661 acatcgttga ggatctaaaa aaaggtgttg cgatcgctaa gattgcaaaa gactacaatg
      721 ttacgagaca aaccgtatat agaataaaaa aagacagcat ggtcaatcac gaatagttga
      781 tcatgctgct gtttctattt tatttgctta ctttttgaaa aactgttcta actttcatca
      841 ataactcctc aactaaacta tcatgcgctt tttccaaaaa aatacactct ctggcttcat
      901 aatcaattgt cgttagttga tctaataata cttttcctgt tgtcttcatt tcatattctg
      961 agtttatatt tacatatagc ggatagtttc tagttgtact agaaattgga gcaactgtaa
     1021 caacgttact ataatccgca ataatatcat gacttaaaac aatataaggt cttttacctg
     1081 tctgttcgtg tccagatcga ggtgctgtat tcaataatag aatatcgcct tgtctaggtt
     1141 ttctcaccat ttttcattac ctactgcttc acctaaattt tgaatcgtca catgatttct
     1201 ttcatcagtg taattttcaa ataactgttc aattgttaat tcattttcta tattagccgc
     1261 ttcgataatt agccgcttct gaccgtttac ttcttcttgt attatcttca ctttgtcttc
     1321 tgtatgaatt ccaagatatt ccataatata ctttggcaat ctaaggccat tactatttcc
     1381 ccattttttt ataggtaaca tttccatagt agacacctct ttctgtatat acatagtata
     1441 acatacat
//
LOCUS       DAXWLF010000275         1423 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXWLF010000275 DAXWLF010000000
VERSION     DAXWLF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.281"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            98..1390
                     /locus_tag="ACGE4U_002742"
     CDS             98..1390
                     /locus_tag="ACGE4U_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFF8394825.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          463 a          292 c          257 g          411 t
ORIGIN      
        1 acaccagcat cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga
       61 attaatttgg cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa
      121 agaaacatta gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga
      181 aaaatacaaa gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga
      241 atgcatccac tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt
      301 ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg
      361 cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca
      421 ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc
      481 aatgacagag atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa
      541 gcgattttat cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga
      601 tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca
      661 aacacagcgc ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt
      721 tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc
      781 tccttatgta tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt
      841 tcacatcgtc caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca
      901 actattaaaa agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg
      961 gaaacttctt caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg
     1021 cttcaaagct catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc
     1081 tttgcgtgta ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta
     1141 cgtatcgttt gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac
     1201 cttgagaacg ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc
     1261 aaatggccca cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg
     1321 ctttcgtagc ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat
     1381 tttcaactaa aaaagatcaa gaggaactga tccacttgat ctc
//
LOCUS       DAXWLF010000276         1416 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXWLF010000276 DAXWLF010000000
VERSION     DAXWLF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.282"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            55..429
                     /locus_tag="ACGE4U_002743"
     CDS             55..429
                     /locus_tag="ACGE4U_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8394826.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(543..1172)
                     /locus_tag="ACGE4U_002744"
     CDS             complement(543..1172)
                     /locus_tag="ACGE4U_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8394827.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1306..1378)
                     /locus_tag="ACGE4U_002745"
     tRNA            complement(1306..1378)
                     /locus_tag="ACGE4U_002745"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1343..1345),aa:Thr,seq:ggt)
BASE COUNT          483 a          216 c          250 g          467 t
ORIGIN      
        1 tattaatata tgctagcatg cttttgtaag aaaaagaaag agggcgtatt agttatgtat
       61 cttaaaatta cggaacaagc acaagaaaga ttacaaaaat atattgatga aggcgcaaca
      121 gttatattgg atttagatga tggcgttggg gaatattcta aaatgggtgt ttgctcttta
      181 gatactagtt ttcgtttact tcttcttgat aaagaacaga gaaaaaatga ttataaactc
      241 caattagata gtgatattgg ggatgtttat ataaaagatt attcaaaaat gtacatggat
      301 gaacatatga ccctttcttt agatccacgt ctaggtgtat ttactcttga aagcccaagt
      361 ggttctttag acagtcatgt tcaattagta gatctacgac cagaaaaatt ctctggaata
      421 aaacaataac tttctctcta ttgtccataa aactagtctc aatcaagctg tattgaaaaa
      481 acaagacaac attcttacca tggtaaataa aaagacgaca cattttgtcg tcttttttta
      541 aattaactat tttcgataaa agatcgcata gcttgtgcca ttatttctgg cttttctcta
      601 ttcaattcat gttttccgtt tttaataaag agtagccggc cattcgttga aagtttatat
      661 aaacgttttg ctgctggttt attcgcatga tctttggtac cgcataaaat caatttagga
      721 agtcgtgatg tacgaagctg ttttttgcag tctaatgttt caagtgaacg aagtagggta
      781 agggtttgtt ttttagatag acctaatttt tcaaatatct tagaaggtac gcagtaaaaa
      841 taaatattct ggaaagtaag caataagcga ttaggagact caaattgtgg agcgactaaa
      901 acaacctttt ttaaatatgg agaaggagtt gcaagatagt ttaaagcaat aacagcgcct
      961 aaagataatc cacataaaat aaaaggttcc tttatctgat tcaattgtac ttcgatgtat
     1021 ttggttaagt cgtctaaagt agcagtgtct gtgctgttta aatgattgaa caatgaaaga
     1081 cttttgcaag aataactatt gggtagataa gagattacat ctttccatga ttgttcgtct
     1141 tggcctagac catgcaaaaa aacaattttc atattgctct cctttttata gttatcatat
     1201 agactttctt tgtactatta aaatataaac attcatttct tacgtcataa aataaaaaaa
     1261 cagcgcagac acattgttaa accaacatgt tgcactgttt aaggaagcca cttgccgggc
     1321 ttgaaccggc gacctcttcc ttaccatgga agcgctctac cagctgagct aaagcggcat
     1381 tatcattaat acaaataata ggataacgaa tattaa
//
LOCUS       DAXWLF010000277         1409 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXWLF010000277 DAXWLF010000000
VERSION     DAXWLF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.283"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..1359
                     /locus_tag="ACGE4U_002746"
     CDS             <1..1359
                     /locus_tag="ACGE4U_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFF8394828.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DAXWLF010000278         1409 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXWLF010000278 DAXWLF010000000
VERSION     DAXWLF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.284"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            35..1117
                     /locus_tag="ACGE4U_002747"
     CDS             35..1117
                     /locus_tag="ACGE4U_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8394829.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
BASE COUNT          445 a          257 c          284 g          423 t
ORIGIN      
        1 ttaatttaaa aaaataatgg aagaagtttt ttatatgaca aaaaatttaa ttgttcgcgg
       61 tgcccctcaa gagtatgaaa taaaagaaaa cgcatgggat tcactccctg aacaccttta
      121 caaaaggcat attgacaacg ttttgattct ccatggaaaa acatcttgga aagcagtaaa
      181 agatgttttt ccagatttat caataacaaa aatttttgaa tattatggtg gagaatgtac
      241 cgatccactt gctgacagtt ttgtttcttt atgcaaaaag aaggatttca atggtattat
      301 tgcagtcggc ggcgggaaag tagcagatct aggaaaattg attgcctata agacaaactt
      361 accaatcatc atcttaccta ccttagctgc gacgtgcgca gcttatactc cacttagtgt
      421 ggtctataat gaagaaggag tgatgcttcg ctatgatatg ttccctaaag caaatgattt
      481 agtgttgatc gatccaaaga tcattttgca ctcccctcgc gagatgatga ttgccggtat
      541 tggtgacacc ttagcaaaat ggtatgaatc tcacgcaatc atctctcagt taactatcaa
      601 gcctatcgaa gtccaagtag cggaatttgc agcgttacga tgccgcgaaa tccttttaga
      661 gaaaagtgca gaggcaatgg aagcgatgga ccagcaaaaa atcaatcaag cttttatcga
      721 tgtcgtggaa acaaacattt tattggctgg tatggttggt gggtttggcg atgactacgg
      781 ccgaacagct ggagctcatt ccattcacga tgcgatgacc gtactccctg atagccatca
      841 gcaacttcat ggaaacaaag ttgcatacgg tatccttgtc cagctgatga tcgaaaataa
      901 attagcagaa atcgaacacc tactgccgtt ttatcatcac ttgaacttac caacgagttt
      961 agctgatatg ggtttacatt taacagaaag tgactatcaa gccgtagctg aacgtgcatg
     1021 tttacctgat gaaatgatcc acttcatgaa agaaacgatc acaccagcac tggttgtcca
     1081 gaaaatgaaa gagttagaac aagtgacaca gggatagtta gccccttata ccatggcagt
     1141 cagctttttt atggatcggc tcaatggtaa aaaaaaagaa gtggaaaaaa ttgacattcc
     1201 acttcttttt tttgcgctaa aattatgatt tcaaggaaat atccgttgct catcttattt
     1261 tagagaacgg aggtgctaag tatgctttat actgaagtat ttttagctgg attcttcttt
     1321 ttaacattaa gcgtcttttt tgatcctcca atcggttatt tctatgaaat ttctatgaag
     1381 ttgattgaat tatgagcgct tttttagta
//
LOCUS       DAXWLF010000279         1408 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXWLF010000279 DAXWLF010000000
VERSION     DAXWLF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.285"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(12..1043)
                     /locus_tag="ACGE4U_002748"
     CDS             complement(12..1043)
                     /locus_tag="ACGE4U_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8394830.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          422 a          231 c          241 g          514 t
ORIGIN      
        1 atacatttgt tttatatgga agaaacccga acgaatgccg ctaattcgtg ataagaaaaa
       61 tcaaccatct tttcccagat atcttgaaaa gaatgggatg agaggtatcg attaccacat
      121 aaataattta ccgcgtcagc atcatctaaa tatagttctg cataataacg atatgttttt
      181 ctgcatagtt ctggcgatac gtttttatat actgtaattt tgctcaaaat ttggcgaata
      241 gtccgaggag aaatttgctg atcatgtgag gaacgaaaaa caaaagaatt ttcgtccgta
      301 ttttgtgctg ttaacttaaa atagttctcc agaaatggca tcgcttcttt tggtattggt
      361 actttattca gtctgttttc ttttctattc agattaagaa tctgcttggt caaagataca
      421 tctttacgtt taagttctac tacttcatgg atctttaatc cgcaatacag aagaatggca
      481 gtaattgcaa ggttcctcgg tctattcttt tcttcaaagc tagcttctct gcttgtttga
      541 aagccttcaa tcgggataaa caaatgctca aaatctttga aaagttctgc atcgatatat
      601 cgcggagctg ctttggttgt ttccggtaaa cttgtaccca aaagagaaat ctcttcttca
      661 atcagtcctt tttgatacaa aaatcgtaaa aaatgattta atgaatattg cttccttgaa
      721 atcccgtaat agctattttc aaacaattgc tccgtttctt ggttcgctgt agtttggaac
      781 gaacgagtat gtcgatagat tttagctaag aaattctgat aatcttgttt ttctaaatgg
      841 ataatcagct gatcatccac ttcttcagaa aatacttttc tcaaataatc agtaaacaga
      901 tgaaaatctt ttgcgtattc atagcgactg cctaatgaaa gggaagaacg attttcttct
      961 aaaaactgtt gaaagacgga agggagcaag tgtgtacgtt gctttaactt ttcttgcagt
     1021 tgttttctag ttttgctgtc cattacaaat tccctccaat agtgttcatt ttctatattt
     1081 taccgcattt catcaaatga agatagacag tttgtcttga tcttaggaag caaattttta
     1141 actaagtact agctaaaaga tatgaagtaa gaagatacat ttttagttat tttattagtg
     1201 cagtcattct tttgatcttt taattgattt atcctctttt tagctatgtg gttacttcat
     1261 gagcaatgaa atggatttgg ttcgttctaa tggcaggagg ctctttcttt tatttccgaa
     1321 aaattagaaa acattttttc agaatcgatt ggctctccat ttattcaata atggtttgtc
     1381 taacgatatt gttgggataa ttcttaaa
//
LOCUS       DAXWLF010000280         1407 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXWLF010000280 DAXWLF010000000
VERSION     DAXWLF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.286"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            99..791
                     /locus_tag="ACGE4U_002749"
     CDS             99..791
                     /locus_tag="ACGE4U_002749"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFF8394831.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            803..1348
                     /locus_tag="ACGE4U_002750"
     CDS             803..1348
                     /locus_tag="ACGE4U_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFF8394832.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
BASE COUNT          475 a          245 c          312 g          375 t
ORIGIN      
        1 ggaactcttt tttgatgaaa tctcttatta ttgttatcct aatgttatgt gtggtttttt
       61 tagcaatcgc atttatatag aaagaaggaa ttaaaaagat gaaaatcggt attatcggcg
      121 caatggaaga agaagtaaaa attttgagag agaacctaag cgaaccatta tcatgggaac
      181 gcgcaggcgc tttatttatt tcaggttcat taggtaacca cgaagtgatc gtcgtacgtt
      241 ccggtatagg aaaagtctta gcatctatta ccacaagtct gttgatccaa caatacggcg
      301 tgaacatggt gatcaataca ggttcagctg gcggaatcgg tgaaggctta caagtaggtg
      361 acatcgttat atcagacaaa gtagcttatt ttgatgcaga tgcaacagga tttggctaca
      421 acccaggaca attaccagga atgcctctat attatgaagc aagcacatat ttgcgcagtg
      481 aaatggttaa agcagcaaaa gcaacgaatt tgaacgcaaa agaaggattg atcgttacag
      541 gtgatacatt tgtcgactcg ccagataaaa tcaaagaaat cttaacgaat ttccctgaag
      601 cattagcttg cgaaatggaa ggagccgctg ttggacaaac agctcgtcag ttcaatatcc
      661 catttttgat cgtacgtgca atgagcgaca cagcagatca ctcggcgaca caaagctttg
      721 acgaatttat cgaagacgca ggcaaacgat cagcagaaat ggtgatcgaa tttgtcaaac
      781 atctcgtata aggaggacta agatgaacgc attgatctcg attgactaca catatgattt
      841 tgttgcagag gatggaaagc tgactactgg gacagcagga caagtaattg aaaaaaagct
      901 gactgctcat acaaagaagt ttattgatca aaaggatttt gtcgtttttg ccattgatgc
      961 gcatgatcca aaagattcct ttcatccgga gaaccgctta tttcctccgc ataatgtgat
     1021 aggaacgggt ggacgggaat tgtacggctc tttaaaaagt ttataccaag actatgagca
     1081 agaggaaaat gtttattgga tagacaaacg gcattattcc gcttttagtg gaacagatct
     1141 agacatccgt ttgcgagaac gccagatcac agatatttat ttgaccggcg tatgtacaga
     1201 tatctgtgtg ttgcatacag cagtcgatgc gtataatcta ggatacaaac tccatatttt
     1261 caaagatgcc gtggcaagtt tcgatcccgt tggacatgaa tgggcattga gacattttga
     1321 gtccacacta ggcgcagaaa ttttataaaa aaacaaagtg atttgataaa ataaaaagca
     1381 caaaataata aaagaagagc ataaaac
//
LOCUS       DAXWLF010000281         1405 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXWLF010000281 DAXWLF010000000
VERSION     DAXWLF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.287"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            118..>1405
                     /locus_tag="ACGE4U_002751"
     CDS             118..>1405
                     /locus_tag="ACGE4U_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFF8394833.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKL"
BASE COUNT          561 a          174 c          238 g          432 t
ORIGIN      
        1 caaacatata ttttttatat tacaatattt caacaaatta aaaaaaatta tatcttctta
       61 aacaatgata atattaaaat gcaagcgctt gtaaaaaata aggagaagag gtaaaaaatg
      121 aagaaaaaac tattaacttt atttcttgtg atgtctttct caatcgtttt gagtgtgtac
      181 tacaatacta ttgttttttc agaacaaata aactacacag aagaacagta taatatagaa
      241 aaagaaaaag ctttaaaagt tggttatagt gaagaacagt ttaagcagat tatggaaata
      301 cctacaaatt tatcaaatga aaatagtgaa acaagaatag taaattatac tatgacttct
      361 aatcaaacca aagtcattaa taaagcaatg gagcaaattg gaaagccata cgaatgggga
      421 gcatcgggac caaattcttt tgactgtggt ggtttagtga aatatgttta caaacaagct
      481 gtaaatattg aactgcctat gggaaccacc aatcaagagc aatacggtac agaggtaagt
      541 ttaaattcac ttaagcctgg tgatttatta ttttacggca atcgaggtgc aacttatcat
      601 gtaggtatat ataaaggtaa cggtgtaatg attcatgcac cgcaacctgg agaaactgta
      661 aaagaggtta acatacaata tttttatcct aactttgcaa aaagaatttt gcctgatgaa
      721 cctgactacc cttatattga ctataataaa atggtaactg ttacaaaagc ttggtcaata
      781 tggaatgatt tacaatttag tcatgaaatt aagaaagcta ttattggaga taattacaag
      841 atagggaagg tttatactaa tccagaaaat aataataaat atggagaaat attagtaagt
      901 aataaagtat atggatacat taatttttat gctgttaaag agttgacctc tgttcaaata
      961 aacagatatt taacttctaa agatagtggt caaccaactt ggggaaatct agagtgtact
     1021 ataagtaaag gacaaactac aaaagataaa atatattttg ttaaaggagc atataattta
     1081 ggggatggaa agtatctata ttctatttat aaagatcagg attcctctga atggttaggg
     1141 tatttaaaag cacatgttag tttagcctat actcctattg aagaaattaa taaaaacgta
     1201 actgttacta aaaattggtc gatttggaat aatctacaat gggaaaagga aattgaaaag
     1261 cctcagattg gttctgtatt ttcagctaga ttaaagttaa ccaatgtatc gaataatgct
     1321 gtctattaca agctttataa atctggaaaa ttttatggtt atatcaatgc tgaggctgtc
     1381 aaagacctta caacaacaaa gctta
//
LOCUS       DAXWLF010000282         1394 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXWLF010000282 DAXWLF010000000
VERSION     DAXWLF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.288"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            33..1372
                     /locus_tag="ACGE4U_002752"
     CDS             join(33..474,474..1372)
                     /locus_tag="ACGE4U_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFF8394834.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMAQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          579 a          181 c          245 g          389 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatggc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agttcaactt
     1381 ttggggttca gttc
//
LOCUS       DAXWLF010000283         1375 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXWLF010000283 DAXWLF010000000
VERSION     DAXWLF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.289"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..903
                     /locus_tag="ACGE4U_002753"
     CDS             <1..903
                     /locus_tag="ACGE4U_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFF8394835.1"
                     /translation="LEELPEGTKVNDVTPEGVIDTTQPGEYEGTIEVTYPDGTKDTVK
                     VPVEVVDNRNDAEKYTPEVEKEEVNYGGTIDLTDNVTNLEELPEGTKVNDVTPEGVID
                     TTQPGEYEGTIEVTFPDGSSTTVKVPVTVKDKVDWVEINPIPTIDPEQIVPEEVHKGE
                     EADLTDNILNLPEGSKVEVVTPVDTTQVGEHTGEVKVTFPDGSSVVVKIPIIVKNMDD
                     MITNNQMTRVEKNSVVLDVINKSKSLSSSKIFGKSDNMYVETSNKELPKTGEENTTIL
                     SILGMLSISFCVLLFRLFKKREEN"
     gene            1133..>1375
                     /locus_tag="ACGE4U_002754"
     CDS             1133..>1375
                     /locus_tag="ACGE4U_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394836.1"
                     /translation="MIILCFENKGYFFEISDIAGFVEVERKKQCSSATLNTYDYVLIN
                     IVKQLSEQQKKLIYLINSKNFNTTLSNLIEMDAKISD"
BASE COUNT          545 a          187 c          274 g          369 t
ORIGIN      
        1 ttggaagagt tgccggaagg cacgaaagtg aacgatgtta caccagaagg tgtcattgat
       61 acaacccagc caggtgagta tgaaggaacg atagaagtta cgtatccaga tggaacaaaa
      121 gatacagtga aagttccagt ggaggtagtg gacaatcgaa atgatgctga aaaatatact
      181 ccagaagtag aaaaagaaga agtcaactat ggaggaacga ttgatctgac agataatgta
      241 actaatctgg aagagttgcc ggaaggcacg aaagtgaacg atgttacacc agaaggtgtc
      301 attgatacaa cccagccagg tgagtatgaa ggaacgatag aagttacctt cccagatgga
      361 tcaagtacaa cggttaaggt gccagtaaca gtcaaagata aagtagattg ggtagaaatc
      421 aatccaattc caacaatcga tccagaacaa atcgtgccgg aagaagttca taaaggagaa
      481 gaagctgacc taacagataa cattctcaac cttccagaag gaagtaaagt ggaagtcgtc
      541 acaccagtgg atactacaca agtaggcgaa cacacaggcg aagtgaaagt taccttccca
      601 gatggttcaa gtgtggtagt aaaaatccca ataattgtga aaaatatgga tgatatgata
      661 actaataatc aaatgacgag agtagaaaaa aattctgtag tactcgacgt tataaataaa
      721 agtaaaagct tatcttcatc gaagattttt gggaagtcag ataatatgta tgtagagaca
      781 agcaataaag aacttcctaa aacaggagaa gaaaatacta caattttatc tattttaggg
      841 atgttatcaa ttagtttctg tgttctttta tttagattat tcaaaaaaag agaagaaaac
      901 taaagattaa aaatagcaaa cggagattat ttccgtttgc ttattttcta aaaaaataaa
      961 aaacgaacac tcatgttggc gatttagatg atataattta tgtgacacaa agaaaactta
     1021 gaaatatgtt tcaagagaaa taattactgt ttgtctaatt tcatcatgtg tatgaaagaa
     1081 accaataaat ttgtcttttt tcaaagaaaa cttattttaa taatttgaga ggatgattat
     1141 attgtgtttt gaaaataaag ggtacttttt tgaaatctca gacattgctg gatttgttga
     1201 agttgaaaga aagaaacagt gtagctcagc tacattaaat acttatgact atgttttaat
     1261 taatattgta aaacaattga gtgagcagca aaaaaaattg atttacttaa taaactcaaa
     1321 gaatttcaat actacactaa gcaatttaat tgaaatggat gccaaaattt ctgat
//
LOCUS       DAXWLF010000284         1360 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXWLF010000284 DAXWLF010000000
VERSION     DAXWLF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.290"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            223..788
                     /locus_tag="ACGE4U_002755"
                     /pseudo
     CDS             223..788
                     /locus_tag="ACGE4U_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          480 a          170 c          299 g          411 t
ORIGIN      
        1 attgggaaat atctgaaccg attgttaaca attttggtga ttagaaaaca attacgataa
       61 atagaaacaa gccaaatcat cagcaagtaa aagctgatga tttggccttt cttatacgag
      121 tagtaggaat tcatagcgaa tattattacg atcagtagat attttgatat taaaggagta
      181 ttacgaacta gtatgtgaga atgtagaggt gatttgcgaa taatgagaaa tttatcaaat
      241 agaaacaaaa tattaatcat tattgttgtt atagctgtgt ttcatctggg aacgaatgcg
      301 gttttaagta gaataatatt gggtcctaag ccaccaagac ctgcgattac ccgaggggag
      361 tttgatttta gattagaata tgaagtagat ggggaaagga ttgtgataga agatacaatt
      421 gttgcattat ttgatggatt ttctgcagat gcaggtagca tggcatggta tcgcacgtgg
      481 cgtttgcatt tggcgagtga caagagggca caacaattat tgttacatga gttagatgat
      541 ggtcgaaaaa tatattatca ttttggagga gctggatttt atatggggaa gttagtagca
      601 gtgtaactgt aacaaggaaa gatttgccag gtcttggcat tcgaaactcc atattttata
      661 tcgagcgtgg gagagataca tctattggga acaggaaagg gttaactctt gaagagttat
      721 ataatgactt tggaatccgt cttgttagtt gggaacacaa tccaccaata gaaaatcgct
      781 ttgaataatc gaaatctatt aatccatttc atcagccagt gaatgttgta atggaacaat
      841 ttggtttagt gttcaaaacc attgaaatac taggctgtga tcagcaaaaa tgtatctgaa
      901 taaaaaagag agcaactatt aaatgagagg agttaggagt tgttagcttt atacgaatca
      961 aagtggcaaa tacagatgcg tatgcatttg tgtttgccat cttgcgtttg gtagacgaga
     1021 taggtataag ctaaagaaac aacttgttta aaataaacga cctagctcaa caaacgacca
     1081 tctaatcaac taacagcata cataaaagca actatttttg aagtagaatt gaaatcggga
     1141 taacttaaaa agtgattggg atactagtgt gaatcaccgt acataaatat tactcgttaa
     1201 gggaatttga gaaattttgt atgaaaattt aagaattagt tagtatgcgc aagtcaaaaa
     1261 tatgaataaa ttatttggtg gagataattt gctcagcttg tcataagaaa tgagataatc
     1321 tggcagatgc aagcttttgg acaccattta ttcggaataa
//
LOCUS       DAXWLF010000285         1353 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXWLF010000285 DAXWLF010000000
VERSION     DAXWLF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.291"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(134..424)
                     /locus_tag="ACGE4U_002756"
     CDS             complement(134..424)
                     /locus_tag="ACGE4U_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394837.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(542..802)
                     /locus_tag="ACGE4U_002757"
     CDS             complement(542..802)
                     /locus_tag="ACGE4U_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394838.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(939..1352)
                     /locus_tag="ACGE4U_002758"
     CDS             complement(939..1352)
                     /locus_tag="ACGE4U_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFF8394839.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          502 a          226 c          204 g          421 t
ORIGIN      
        1 ttactatgaa taacataata agaaagtaaa gtggattgat ttgagatctt tttatacttc
       61 aaaaaagata acacccaact ttcaaaaata tgttttggaa gttgggacaa acgagttaat
      121 acattttaat acattatttt ttattcaaaa aaaatagcac aatggcaaag atcgtaagag
      181 agatcataag ccccaatgaa tgactaaaaa gaaaagcaga atcacatgcc atatcatccc
      241 atatttcttc aagaaaataa ataagcggga aaaagggaag gaatagtaga acatcgatcc
      301 agcaccattt ttttcttctt ttgaaataac ataaaacaat aaacatgaac gtgcagggaa
      361 gtaaaaacaa agctttactc ataactaaca aaataccagc tccttttatt tttttctccc
      421 ccacattcaa agagtaacaa tggtatgtac ttaataatag aaaaatggat caacttgtat
      481 gaataacagc tgaaaagatc tgcaccaaat aaaaaacttc cagccaaaac tgactggaag
      541 tttatttttt tctgaaatat tctaacacaa atatgaaaaa gatggccgaa gctaataagc
      601 cgatttcgta gcaaaacaat gagataccag agtcgtaata ataaaatcct ctaatcttat
      661 ggataaaata gatcatcgga caagcagata agagtaagta tttgttaggc cttttgaata
      721 agtaaaagaa agcactggtc attatgataa atggtatcca aaacaaaagt ttacttaaga
      781 caatcaaatg atcaactccc atatttttta ccaagagaga tcataacatc tttatcgtaa
      841 aaacaaaata tttttttgct cattttgaaa atttaaacat actcgttctt atccaaatat
      901 ataaaaatcg cccctcattc atcgagaggc gataagttct aatttaactc aatttttttt
      961 gtttcttttg gctgataagc tgttttaggt aataccaatt tcagcacacc gtttttaaag
     1021 gttccagtga ttttttcttc atcaacatta ggaagataga atgaacgttt gtaagagacc
     1081 gaatgtcttt ctttccgtac atagttgcca tcttctttat cttctgtatg ggactcatga
     1141 ttggcggaga tcgtcaaagt attatctgaa tattcaactg tcgtgtcttc tttgtcacag
     1201 ccaggcatat cagcagttag tttgtattct ttttcatttt cgactaaatc tacttttgga
     1261 taacttgaaa cacctaaaaa atcattgaat gctggactaa acacgtcatt gaaatcaggg
     1321 aacatgtcgc gaatgctagg tacatttgcc att
//
LOCUS       DAXWLF010000286         1336 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXWLF010000286 DAXWLF010000000
VERSION     DAXWLF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.292"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(193..486)
                     /gene="rpmA"
                     /locus_tag="ACGE4U_002759"
     CDS             complement(193..486)
                     /gene="rpmA"
                     /locus_tag="ACGE4U_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFF8394840.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(499..843)
                     /locus_tag="ACGE4U_002760"
     CDS             complement(499..843)
                     /locus_tag="ACGE4U_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFF8394841.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(870..1178)
                     /gene="rplU"
                     /locus_tag="ACGE4U_002761"
     CDS             complement(870..1178)
                     /gene="rplU"
                     /locus_tag="ACGE4U_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFF8394842.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(1198..1274)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
BASE COUNT          387 a          291 c          221 g          437 t
ORIGIN      
        1 gatagcaaac aatatagctt ccatcaattg aaaacgatag ttatcaaaac atcctgattc
       61 caaattggca aaaaaatatt aagtgtaaaa agacaaagta aacttcgtag cctccactac
      121 aacttttatt tttataaaca aaaaaggctg tgacaaaagt cttgtcacaa cctctttaat
      181 cataagattc aattaagctt cttgagctac tggataaaca gacacttgtt ttttgttacg
      241 gcctttacgt tcgaaacgta ctacgccatc aacttttgca aataaagtgt cgtctccacc
      301 gatacccacg tttacacctg gatagatttt tgtaccgcgt tgacggtaaa gaattgatcc
      361 acctgtaact gtttgtccat cagcacgttt agcacctaga cgttttgatt ctgagtcacg
      421 gccgttagat gtagaaccgc cacctttttt gtgggcaaat aattgtagat tcatagataa
      481 caacatggtc tgcacctcct atttatcatt aattgtttta atttggatat attctgagtt
      541 ttcagcttct atcgattgta atcctaataa aagattttct aatagaatct cagcaatatt
      601 gttttgttct tgtgtcattc ctgaagtgac ttcggtataa agataaccgc cttcttcttc
      661 attcatttca acaatcggct caaaaccggc taatgaggca atcccgttga cagtgctgat
      721 cgctaacgca gaaacacccg cacaaacgat atcacttcca taaggaccag catcagcatg
      781 cccggtcaaa gtgaatgaaa cgatctgacc tgcgtcattt cgcttaaatg ttcctttgat
      841 catatatagg ccttctttct caaacgctat taagcgttga tcgcgtcgat aaccactttt
      901 gtatatggtt gacgatgacc ttgtttacgg tgtgaatgtt ttttaggttt gtatttgaaa
      961 gtaacgactt tcttttgttt gccgtgtttt tcaacagtac cttcaacagt tgcacctgca
     1021 acagttggag cgcctacttt tgtagattct ccgcctacta agataacttc gtcaaaaaca
     1081 actttttctc ctgcttcaac gtcaagtttt tcaacataga ttgcttgtcc cacttcaact
     1141 tttacttgtt taccaccagt tttaataatt gcgtacatgc tataaagcac ctccttctga
     1201 tatacttaga ctcgccatcc gaagtgacag catatgctgt gcttattact tgttctgagc
     1261 ggttgtagct gtggagtcca caattacaac agtactagct taccagaaca gcaaccgtta
     1321 gtcaactaaa tttaaa
//
LOCUS       DAXWLF010000287         1306 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXWLF010000287 DAXWLF010000000
VERSION     DAXWLF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.293"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            82..>1306
                     /locus_tag="ACGE4U_002762"
     rRNA            82..>1306
                     /locus_tag="ACGE4U_002762"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          399 a          256 c          368 g          283 t
ORIGIN      
        1 ttaataagtt caattgctta ttttcttgat ctaacttcta tcgctagaag aaggatcaaa
       61 acccaaccgt aaggttgata aggttaagtg aataagggcg cacggtggat gccttggcac
      121 taggagccga tgaaggacgg gactaacacc gatatgcttt ggggagctgt acgtaagcta
      181 tgatccagag atttccgaat gggggaaccc aacatcttta ataggatgtt acgattgtgt
      241 gaatacatag cacattcgag gtagacgcag agaactgaaa catctaagta cctgcaggaa
      301 gagaaagaaa attcgattcc ctgagtagcg gcgagcgaaa cgggaaaagc ccaaaccagc
      361 aagcttgctt gttggggttg taggactcca atatggtagt tctttcagat agtcgaatga
      421 cttggaaaag tcagtcaaag agggtaaaaa ccccgtagac gaaatgtgga agacacctag
      481 gaggatcctg agtacggcgg aacacgagaa attccgtcgg aatccgggag gaccatctcc
      541 caaggctaaa tactccctag tgaccgatag tgaaccagta ccgtgaggga aaggtgaaaa
      601 gcaccccgga aggggagtga aatagaacct gaaaccgtgt gcctacaaca agtcaaagcc
      661 cgttaatggg tgatggcgtg ccttttgtag aatgaaccgg cgagttacga ttgcatgcga
      721 ggttaagttg aagagacgga gccgcagcga aagcgagtct gaatagggcg tttgagtatg
      781 tagtcgtaga cccgaaacca tgtgatctac ccatgtccag gttgaaggtg cggtaaaacg
      841 cactggagga ccgaacccac gtacgttgaa aagtgcgggg atgaggtgtg ggtagcggag
      901 aaattccaaa cgaacttgga gatagctggt tctctccgaa atagctttag ggctagcctc
      961 ggaattgaga atgatggagg tagagcactg tttggactag gggcccatct cgggttaccg
     1021 aattcagata aactccgaat gccattcatt catatccggg agtcagactg tgagtgataa
     1081 gatccatagt cgaaagggaa acagcccaga ccaccagcta aggtcccaaa atatatgtta
     1141 agtggaaaag gatgtggggt tgcacagaca actaggatgt tggcttagaa gcagccacca
     1201 tttaaagagt gcgtaatagc tcactagtcg agtgaccctg cgccgaaaat gtaccggggc
     1261 taaacatatt accgaagctg tggagtacac ctttaggtgt attggt
//
LOCUS       DAXWLF010000288         1292 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXWLF010000288 DAXWLF010000000
VERSION     DAXWLF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.294"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..613)
                     /locus_tag="ACGE4U_002763"
     CDS             complement(<1..613)
                     /locus_tag="ACGE4U_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFF8394843.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            901..>1292
                     /locus_tag="ACGE4U_002764"
     CDS             901..>1292
                     /locus_tag="ACGE4U_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8394844.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSV"
BASE COUNT          430 a          201 c          247 g          414 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata
      361 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aatatctgag
      421 tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
      481 aaggtgagtc atccaatctc cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc catagagcga ctcctttggt agggtgatgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccaa caacacaaaa tattatagaa ccctttattc taaaggattt caaggtgtct
      781 gtttgcattt attagaaaaa agaaaaagta ttggcaacag aaggtgtcac caacactaga
      841 tattatagag ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat
      901 atgaaaatat taactgttga agatgataat atgataagag agggaataag tgaatatctt
      961 tcagaatttg gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt
     1021 aatagcgata taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa
     1081 gtgttgaaag aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt
     1141 gatgaagaat ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca
     1201 ttttcattgc cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat
     1261 ttagagaagt ttgaatataa ggatctatcg gt
//
LOCUS       DAXWLF010000289         1273 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXWLF010000289 DAXWLF010000000
VERSION     DAXWLF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.295"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            73..234
                     /locus_tag="ACGE4U_002765"
     CDS             73..234
                     /locus_tag="ACGE4U_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFF8394845.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>1273
                     /locus_tag="ACGE4U_002766"
     CDS             253..>1273
                     /locus_tag="ACGE4U_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8394846.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIF"
BASE COUNT          524 a          113 c          218 g          418 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tca
//
LOCUS       DAXWLF010000290         1253 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXWLF010000290 DAXWLF010000000
VERSION     DAXWLF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.296"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            34..906
                     /gene="scrK"
                     /locus_tag="ACGE4U_002767"
     CDS             34..906
                     /gene="scrK"
                     /locus_tag="ACGE4U_002767"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFF8394847.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          376 a          230 c          302 g          345 t
ORIGIN      
        1 tgatttcatt ctaaaataaa gggagaaatg tctatgctat acggtgcaat tgaagcaggc
       61 ggcacaaaat ttgtgtgtgc cattggcgat gaagaaatga cgatcaagga aagagtgagc
      121 tttccgacaa cgacacctga ggaaacaatg cctttagtca ttgacttctt caaacaatac
      181 caagcagatt tagcagggat tgggattggc tcatttgggc ctatcgatat ccatcgcgat
      241 tccgccactt atgggtacat tacctcgacc ccgaaattgg catggcaaaa ttttgatttc
      301 atcggtacca tgaaaaaaga atttccgatc ccgatttctt ggacaacaga tgtcaatgcg
      361 gctgcctatg gtgagtatgt cttcggcagt ggcaaaggat tgagcagtgt cgtttactat
      421 acgatcggta cgggtgttgg cggcggtgcc ttacaagaag ggcgctttat cgaagggttt
      481 agtcatccag aaatggggca tatgctcgtc gtccctcatc caaaagatga ctttgctggc
      541 agctgtccat ttcatggcaa ctgtttagaa gggatggccg ctggacccgc catcgaaaac
      601 cgcttaggga aaaaaggaca agaggttgca gaagatgatc cttattggga aatcgaagcc
      661 tcctatattg ctcaatgtgc ctacaataca accttgatgt tttctccaga tgtgatcatt
      721 tttggcggtg gtgtgatgaa acaacgccat atgctgcaaa acgtccacga cgcatttgca
      781 aaattggtca atgggtatgt cgaaacacct gagttaagca aatatatcgt gaccccagca
      841 ttagaagaca acgctggaac gctcggttgt ttggcattgg ctcgagaagc ggtcttacat
      901 tcataaacga aaaaaggctc ttcgacattt agtgttggta gagaaagata caactgaaaa
      961 tttagtcata accagttagg cagagtgctt aactggtttt tgttttactt ggaagatcaa
     1021 gccttgaatg agataaattc ttgctcgaaa gttatggaaa tttcgatagc cgtaggccac
     1081 ttgtattgtg caatagtttt ttacaaaaaa aagggggtgc ggatattttt ttggaccttt
     1141 ttaacaaata ggttctgttg caaagtttta aatctactat cagataaggt agaataatag
     1201 aaaaagatag caggaggaat gacgatgagt cattttaaag gaaagcaatt tca
//
LOCUS       DAXWLF010000291         1222 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXWLF010000291 DAXWLF010000000
VERSION     DAXWLF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.297"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..489)
                     /locus_tag="ACGE4U_002768"
     CDS             complement(<1..489)
                     /locus_tag="ACGE4U_002768"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394848.1"
                     /translation="MFDKNINSEIGKRIQEIRVSLGYTLEEFGENLTPPASKSNVSRW
                     EKGKNIPNKARLLSIAKLGGVSTEYLLTGNSQKTKKYIKYLFNEIFFDNGTFHNKFKD
                     FGKDRDRLGLYDLFLAVSKTYSLDKSPSLKDNFVEMIIDDVYKKLLQTDKSDDQLILG
                     FIY"
     gene            653..859
                     /locus_tag="ACGE4U_002769"
     CDS             653..859
                     /locus_tag="ACGE4U_002769"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8394849.1"
                     /translation="MKPKDFRKFCGLSQSELAEKYGISVQAVRNKESGKTNYSPEEMI
                     ILKYLVKEKGIENITIDQIFFNEK"
     gene            910..1191
                     /locus_tag="ACGE4U_002770"
     CDS             910..1191
                     /locus_tag="ACGE4U_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394850.1"
                     /translation="MKSEIQVILPSDLDQELRRYFYRVAKEAVDQVRIETSLSKEVFR
                     LGEACKYLSCSRSTLKKFIREKGLKVSIIGDCQFIRKEELARFIKEYEV"
BASE COUNT          441 a          158 c          222 g          401 t
ORIGIN      
        1 atatatgaaa cccaggatga gttggtcatc tgatttatca gtttgcaaga gttttttata
       61 tacatcatct ataatcattt ctacaaagtt atcttttaaa gaaggtgatt tatctagact
      121 atatgttttc gatactgcta aaaataagtc atatagacca agcctatctc tgtctttacc
      181 gaaatctttg aatttgttat ggaaggtacc gttgtcaaaa aaaatctcat tgaataaata
      241 cttgatatat ttcttagttt tttgagaatt acctgtcagt agatattcag tagaaacacc
      301 accaagttta gcaatactta gtagccgcgc tttgttgggt atgttctttc ctttttccca
      361 tctagaaaca ttgcttttgc ttgcgggagg tgtcagattt tcgccaaatt cttcaagagt
      421 ataccctaaa gaaacacgta tttcttgtat tcgttttcca atttcagaat taatgttttt
      481 atcaaacaaa atggtttcac caccttcatc tattctattc tacagtttac actacattct
      541 catataatga aatgaaaaag tagataaaag ttgaatttta cgttgcggta tgaataaata
      601 tatgttattc ttttagtgac gaaaagttga aaagaaggag gagtgaggag tcatgaagcc
      661 taaagatttt agaaagtttt gtggattatc acaatctgaa cttgctgaaa aatatggcat
      721 ttcagtacaa gcggtaagaa acaaagaaag cggtaaaaca aactattctc cagaagaaat
      781 gattattttg aagtatcttg tgaaagaaaa aggaattgaa aatatcacaa ttgatcaaat
      841 tttttttaat gaaaagtgac gaaaagttga atttgaatac atgtagtcaa attagtgggg
      901 gcaaagaaaa tgaaatctga gattcaagtt attcttccat cggatttaga tcaagaattg
      961 cgaagatatt tctatcgagt tgcaaaagaa gcagtagatc aagttcgaat tgaaacatct
     1021 ctaagcaaag aagtttttag attgggggaa gcttgtaagt atttaagctg tagcagaagt
     1081 acattaaaaa aatttatcag agagaaaggt ttaaaagtaa gcataatcgg agattgtcaa
     1141 tttattagaa aagaagaatt agcaaggttt atcaaagaat atgaagtata aaaagttgcg
     1201 tggcgtgctt ttgatagaaa aa
//
LOCUS       DAXWLF010000292         1207 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXWLF010000292 DAXWLF010000000
VERSION     DAXWLF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.298"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            24..872
                     /locus_tag="ACGE4U_002771"
     CDS             24..872
                     /locus_tag="ACGE4U_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8394851.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          406 a          205 c          202 g          394 t
ORIGIN      
        1 tattatgaaa atgaggaatt ctcatgagcg tctccataag actacaaatc aaaacaatgt
       61 acaacgactt cagcccaaaa gaacaggcta tcgccgatta tattttagaa aatccaagca
      121 aagtaagtca tagtcctatt agcaatttag caagtgaact gggcattgct gactctactt
      181 tttttcaatt tacaaaaaaa cttggttata atggatttaa agattttaaa atggccatgt
      241 tgatccaaga aaatgatcta tctgctattt ctatccacga aaatattcag aaaacagata
      301 ctgagctcac tatggcgcaa aaggtgttcg agtctagtat ttcaacttta aatgacacaa
      361 aaaagttgtt acaacaaaag gatctaaaaa aagcggctac tattatcaat gaatctaaac
      421 gtgtctattt tttcggtgtt ggcggttcag agattgtagc aactgacgct tatcataaat
      481 ttttgcgttc ccctatcgca acttctcaca gcacggatta ccatattcaa ttgatggaag
      541 cttctttatt gacggaaaaa gactgtgctg ttttgatttc tcacacaggg caatcaaaag
      601 aaacaatcca tattgctgaa accgtaagaa aaactggagc aaaaacaatc gttattacta
      661 gccaagctaa ttcttccctt gctaaacttg gcgatgtcgt ttttatttct atttctgaag
      721 aaacagaatt ccgatctgaa gctttagctt cccgtatttc tcaactaagc attttggact
      781 ctctatatgt gattttgatg ttttacaacg aaaaagaatc acgtcgttct atcgctaaag
      841 ttcgacatac tatttctgaa atcaaagaat agcaccataa cagaaaaagc gaaaaatcca
      901 taaaacaaaa aaagccacta ccaaatattt ggtagtggct tttttggttc tgcaagggtg
      961 gtacagaaca tcaatagtcg gtgggttaga acgtatgtca tcgcatcttt ggagaaggtt
     1021 gtattggtat tattcgagtt acttgttaga tgcgataaca aagttagtat acagtagtaa
     1081 aaaatttatc ttttcttttt tttattattt ttgtttctta ttttctaaaa aattaaaaaa
     1141 aattttggtg aattaagtat ttccttgata acactggctt ttggcctcat tttaggttat
     1201 catttgt
//
LOCUS       DAXWLF010000293         1195 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXWLF010000293 DAXWLF010000000
VERSION     DAXWLF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.299"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..183)
                     /locus_tag="ACGE4U_002772"
     CDS             complement(<1..183)
                     /locus_tag="ACGE4U_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394852.1"
                     /translation="MNTLKDDFILAKAGSEEAIESILKRFSSLMHQQSWRNGRYDQDC
                     YQECMIAVYLAISKFEI"
     gene            complement(257..643)
                     /locus_tag="ACGE4U_002773"
     CDS             complement(257..643)
                     /locus_tag="ACGE4U_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFF8394853.1"
                     /translation="MSENDERVNAQLICFFEKVIKNTAYSYFERQKKIRELEQFDQFS
                     QHFFTENNIKIKQPVTILDVTFLIEDSRLAEIIPLLKEREQLFLIEKFVFDKTDKEIG
                     ESFGITRQGITNLKHRLYRKIQKLLI"
     gene            complement(704..1165)
                     /locus_tag="ACGE4U_002774"
     CDS             complement(704..1165)
                     /locus_tag="ACGE4U_002774"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394854.1"
                     /translation="MKLEEEKDAIYTQMRMILEERRRLSKDYYELKARLFALDNTNQP
                     NSLIRNEKLAKKSSTNISEEALYQQYICERGNKYRQLIPYSTISLTISSILKDAGRPL
                     SNKEIYKLLISDHKLNISYKNLTHNILPKMNVDNSVNVERAYRGYWQYRLH"
BASE COUNT          373 a          194 c          168 g          460 t
ORIGIN      
        1 gatttcaaat tttgatattg caagatatac tgcaatcata cattcttgat agcaatcttg
       61 atcatatctt ccatttcgcc atgattgttg atgcatcaaa gaactaaatc ttttaagaat
      121 agactcaata gcttcttcac ttccagcttt tgccaaaatg aagtcatctt tcaaagtatt
      181 catagaccgt cctcctttcc taaatttaaa gttatttaga ggaggtggtc gtatacaaat
      241 aatcgttaag tggcttttaa ataagtaact tttgaatttt tctgtaaaga cgatgtttta
      301 agtttgtgat gccttgtcta gtgatcccaa aagattctcc gatttcctta tctgttttat
      361 cgaaaacaaa tttttcaata agaaatagct gttctctttc ttttaataaa ggaataatct
      421 ctgccaatct tgaatcttca attagaaaag taacgtctaa aatagtaact ggttgtttga
      481 ttttgatatt gttttcagta aaaaaatgtt gagaaaattg atcgaattgt tccagttcac
      541 gaatcttttt ttgcctttca aaataagagt acgcagtatt tttaataacc ttttcaaaaa
      601 aacaaataag ctgtgcattc acgcgttcat cattttctga cattctgctc acttcttcca
      661 ataaatattt aaaaggaaaa gggacagaaa gcgaaaaaag aagctaatgc aatctatact
      721 gccaataacc tctataagct ctttctacat tgacactatt gtcaacgttc attttgggta
      781 gaatattatg agttaagttt ttgtaagaaa tattaagttt gtgatcgctt attaatagtt
      841 tatatatttc tttatttgat aatggtcgac cagcatcttt tagaattgag gaaattgtca
      901 aactgatagt tgagtatgga atcaattgtc tatatttatt tcctcgctcg caaatatatt
      961 gctgatacag cgcttcttca gaaatatttg ttgacgattt tttggccaat ttttcatttc
     1021 taattaaact attaggttga ttagtattat ctaatgcaaa aagtctggcc tttagctcat
     1081 aataatcttt actaagtctc ctcctttctt cgagtatcat tctcatttgt gtataaattg
     1141 catctttttc ttcttctagt ttcatctact tctcctttca tgattaacaa ctaaa
//
LOCUS       DAXWLF010000294         1165 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXWLF010000294 DAXWLF010000000
VERSION     DAXWLF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.300"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            101..1045
                     /locus_tag="ACGE4U_002775"
     CDS             101..1045
                     /locus_tag="ACGE4U_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFF8394855.1"
                     /translation="MDTILITGGAGFTGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
BASE COUNT          404 a          167 c          224 g          370 t
ORIGIN      
        1 aactagttat cccaacttca acaaatttta tagtttttac aatataaaaa tggtaaacta
       61 atattatttg atacgaaaat gtgcaattgg agtgaatttg atggacacta tattgattac
      121 tggcggagct ggttttaccg gttcaacgct agcgaattat ttgggaaaag aaaacaaaat
      181 cgtagtagtt gacgatttat caatgggaaa aaaagaaaac ttggatatgg ataaacaagt
      241 gactttcatt gagggggatg tatcagattc tcaattgatg gaaagaatca tgaaagaata
      301 tcagtttgcc tatatttttc atctagctgc tgtagctagt gtggcagatt ctgtagaacg
      361 gcctgtagaa acacatcgtg taaattttga cagtgcgctt ttattgcttg agttagcacg
      421 gaaatatcaa tctaatttaa aacgattagt atttagttca tcagctgcag tttatggtga
      481 tgaaccaaca ctgccaaaaa aagaagaatc agtcatccgt ccattaacac catatgctat
      541 cgataaattt gcagcagaac aatatgtttt ggattactgt catttatatg atgttccgac
      601 tagtgctgtc cgatttttta acgtttatgg tccaaatcaa aatccaaact ctccatatag
      661 cggagtcatt tcgattctag tggatcgtta caaaaaacaa ttggcaggag aaaaagcgga
      721 atttacttta tttggcgatg ggagtcagtc tagagacttt gtttatatag aagatgttat
      781 acaagcactt ttacttgttg ccaaggaaga aaaagcttta ggacagcaat tcaatgtcgg
      841 tacaggaaaa agcactacgc tacttgaatt gatccattca attgatcaaa tcttaggaac
      901 agagctagcg cttaaatatg aagcggagcg ttcaggggat attcgtgatt ccttagcgga
      961 tatttcaaaa attcgttctt taggctatca gcctaaattt gatatattga gcggaatgga
     1021 acggtattta aagacagaaa tttgatgaaa aaatgattgc atctttggat aaataattct
     1081 aaaaagtaaa acaagaatta aagttgtata accaactatt taattcttgt tttacttttt
     1141 atttaggaaa tatataatta aatat
//
LOCUS       DAXWLF010000295         1129 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXWLF010000295 DAXWLF010000000
VERSION     DAXWLF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.301"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..1074)
                     /locus_tag="ACGE4U_002776"
     CDS             complement(<1..1074)
                     /locus_tag="ACGE4U_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFF8394856.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFK"
BASE COUNT          318 a          249 c          185 g          377 t
ORIGIN      
        1 tttaaagctt gaccatggtt tcaaaaagtc aaggaggaat aattcgtcat cggagattct
       61 tcctacaaca ttgacacgtc cgtcgttttt catatccttc aaggcgattt gtgtttctcc
      121 tttgtattga ccgtaccctt cattgtcaat cagtacatcc ccatgatgca tatctcgcgt
      181 attatgaggc gggaaatctt tatctttgta gtatacacga gtcatcgttg aacgaaggat
      241 atattctgag cgatcccctc gataactatg aaggttatca aacaagcaga ttcgttcgtt
      301 ttctgtaata ccttcttccg tatctacttt caatgttgga taatccgcgt taaatgcttc
      361 tgccatttct ttcaattctt cttcacttgc gtacgcattt ccaatagaaa tatcatcaat
      421 caatcctgta aggattaaat gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc
      481 cagcgtgcac aagccatctt gcgtaggcca agggccaaac tcagcagatt gcgaattgac
      541 gaatgccatc gtattcaaat tatattttct gaatttttct gaacaaaaaa caaagtgttc
      601 gtatcctaaa ccagagtatc gatgaggata aaaattatga gaacctaata aattatccgt
      661 gtttggcgaa taactcatga tgttgtcaac gtaattcgtg cccgaactca tattgatctc
      721 gattttgata ccaaaaggat tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat
      781 atctaagcgg accccgtagg cacccatctt atggaaaaaa gataagtcat cgtaagaaat
      841 ctccaactgt tcaaataacg ctggattgat atcaaccatt acttccatgc ctaggctatt
      901 cgcgtaatca actacttctt taaattctga aagaactttt tctttatcat cattgatctg
      961 cagcaaactg gtaaaaaccc gtttataccc atacttatga gctaaatcca agtacgcttt
     1021 gtccttttca aatgttgacc gttctggata aatcgagatt cctaattttc ccatattacc
     1081 atttcctcct gcatcttttc gtttttgtat ccacttacat tatagcaaa
//
LOCUS       DAXWLF010000296         1123 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXWLF010000296 DAXWLF010000000
VERSION     DAXWLF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.302"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            55..813
                     /locus_tag="ACGE4U_002777"
     CDS             55..813
                     /locus_tag="ACGE4U_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8394857.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
BASE COUNT          388 a          201 c          189 g          345 t
ORIGIN      
        1 taaaggaaac tcccgcttta caatagaatt atcctaacta atcggaggca aaatatggat
       61 acaaaattaa gtgaatcgga gaaatatctc tgggatttta ttcaaagtca catactcgaa
      121 atcccctcct attccattgt aaaactaagt gaatatgcca acgtttcaac agcaaccgtc
      181 gttcgtacta tgaagaaaaa aggatatgag gggtttacgt cttttaaaca ttcgttaaaa
      241 gaaaaagaaa atacaaatat caattttgcc gctcttgata aagttgatga aggaataaag
      301 actgctattc ttaaaaatga gcaagaggta atccgcacca tcaatatggt agaaagcggg
      361 aatattgaag atgctattca acgaatcaag gctgctagaa gaatcatgat ttttgcccga
      421 ggtttttcag aattgattgc tcaggaaatg atggtcaaat ttcaacttac cggtaaatat
      481 tgtgagatgc atactgatcc caatatcata aaaaacatta gtagaaaatt atccaaagat
      541 gacatcgtga tctttgtttc actaaacggt gaaacaaagg aattgactac cgcagctgaa
      601 aactgttata aagctgagat cgggacaatt ttgcttactg ctaatcgtca ttcaagtttg
      661 atggaacata tcgagattcc ttttgttggc tttaaatcag aaggctcctt cttcccagat
      721 tatgaggtac gctccagact tccactttct atactggcta gaatcttact tgactcctat
      781 gcactgcgaa catcaggatt cacacagcac taactctata tctgtaagac tgtacctaaa
      841 atatatctgt gtgaattaac taaggttgtc tagaaaaata tttaatcgct atttgtattc
      901 tcgtgctttc tatcattgct ccttctataa cttcttagtt tcaagataga ataatgcatt
      961 gagactacca ggactactta cgcaaatata taataataag aatgtgataa gcagttcgaa
     1021 gttcctgtct aatatttctt gttaattatt atcaatttcc aacaattcta gaattataat
     1081 acgcaaaaaa aggagaccgt ctaaccgtct ccttatagtt tat
//
LOCUS       DAXWLF010000297         1102 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXWLF010000297 DAXWLF010000000
VERSION     DAXWLF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.303"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            2..1084
                     /locus_tag="ACGE4U_002778"
     CDS             2..1084
                     /locus_tag="ACGE4U_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8394858.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          400 a          192 c          213 g          297 t
ORIGIN      
        1 tatcaatgat gatggctcaa gaaaaaaagt ggagaaagtt ggcggagaca cccgaccaga
       61 ggccgaagct gctttacgaa aagttttatc agatattgac gaaacaggac aatacttttt
      121 aggtacggat actcgagtaa aacaatacct tgatttttgg atggaggaat acgttaaact
      181 aaatctaaaa tacaatacct atgaaaacta ccgatttacc atcaaaaatc atataaacgg
      241 ttatttagga aagaaaaaac ttacggatct ctcccctgct cttttacaaa atttcatcaa
      301 tgctgaattt aaaaagggtt actcgaagaa aacaatgact attactcact ctgtccttaa
      361 gaatgcgctg aatatggcgg tttatccttg gggattaatc aagcaaaatc ctatgctgta
      421 tgtaaaaata ccaaaatatg aagcacgacc aacgactaaa aaagatctaa aaatcatttc
      481 tcttgaggac tttgatcata tgctagaaat tactcctgaa ggacatcctt tctatattcc
      541 tttgaatatt ggattttata cgggaatgcg cgttggcgaa gtttgtggtc tgacgtggga
      601 tgatgtcgat ttttcaaatg gaacaattac tgtagagaaa caaatggtaa agaatgacgg
      661 cgcatgggta tatggtacgc caaagacaag cagttccaat cgaacgattt ttattggaca
      721 aaccttgcta gcaattctga aaaaacataa gaaacaacaa ttagaaaatc gaatgaagta
      781 tggaaagctc tacattgatt caaatgcagt atgtacgaag gaagacggtg agctagttac
      841 gccaagtgta gtgaaatgga atactaggag gatatcgaat gcactctccc tctcttttaa
      901 cttccattct ctcagacata ctcatgctac acttcttctc gaaaatggcg caaaaatgaa
      961 agaaatctct gaacgattgg gccacagcag aatttcaatt acgatggata cttactcgca
     1021 tgtaacagat aagatgagaa atgaaacggt cgatatcatg gaaaatctta gaaagaattc
     1081 ttgatttttg ccaccgaaaa aa
//
LOCUS       DAXWLF010000298         1087 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXWLF010000298 DAXWLF010000000
VERSION     DAXWLF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.304"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            12..1052
                     /locus_tag="ACGE4U_002779"
     CDS             12..1052
                     /locus_tag="ACGE4U_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFF8394859.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          442 a          165 c          187 g          293 t
ORIGIN      
        1 gaggatctac aatgactgat tttaaatttt ttaaggcgga tagagtttac aacgaattat
       61 tttatcaatt tccaaaagtc tttattgttt ctgacgaata caaaaaaatg aaagattcaa
      121 ctaagattgc ctatatgctt ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa
      181 tcgatgaaga aggtaatgtt tattttactt atacgacaaa tgaactatgt agagtattaa
      241 actgccaaaa acaaaaagcg atagcaatca aaaaagagtt ggaatccttt ggtttattat
      301 tacaaaagca gatgggattt aacaaacagt tagggaaaaa taatcctaat agactatatc
      361 tagcagaatt aaaagtctca gaaaatgata tctacttact cgaaaaattt gatagagaga
      421 atagggaaaa cgttgataaa tcagagggta tgaaaatcat acccaccctc gacgaaaaat
      481 cagacgctga atcccttggg gctcaagagg gtatgaaaat cataccgtgc caaaacgttg
      541 ataaatcaga gggtatgaaa atcataccag aacttaataa taatatatta gacactaata
      601 gacacaatat agacactgaa aaagaccgcc tacaagatca attgttgtta gacaattttg
      661 agacaattat gacaaacgac agcattgcta cgtttgtccc tgaacgagta ttaaatttga
      721 taaaaacatt ttcttcaagt tacagtgaag ctcaaaaaac cgtccagact attcataatg
      781 caaagaaaaa agctgaaata gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt
      841 attatgtcaa tgcagaacaa gaattataca cgacactgtt aaaagcctat caaaaattaa
      901 aaaccgaaaa agtcgaaaat atccagaacc tgatttttgt ctatgtaaaa aattggttta
      961 tcgaaaaacc aatagctgct aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc
     1021 caagcactat tacgaaagac tggttagagt gaaactttac tgacaaagtg attgttgctt
     1081 catggag
//
LOCUS       DAXWLF010000299         1083 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXWLF010000299 DAXWLF010000000
VERSION     DAXWLF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.305"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            62..1006
                     /locus_tag="ACGE4U_002780"
     CDS             62..1006
                     /locus_tag="ACGE4U_002780"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFF8394860.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          374 a          168 c          231 g          310 t
ORIGIN      
        1 ataaacaaga ctatactttt catgaaagta acaaatagtt atgaaaagga gctgtttatt
       61 catgaaaaaa acaagtcgta aagtagtaat cgtcggtaca ggttttgttg gaacaagtat
      121 cgcttatgca atgatcaacc aaggaatctc taatgaatta gtattgatcg atgtgaatca
      181 agaaaaagca gaaggggaag cattggacct tttagatggt atggcttggg gcgatgaaaa
      241 tgtggctgta tggtcaggtg gctatgaaga atgtaaagat gctgatatcg ttgttatcac
      301 agctggtatc aaccaaaaac ctggtcaatc tcgtttagat ttagttaaaa caaatgcgtc
      361 aatcatgcgc caaatcgtaa aagaaatcat gggatcagga ttcgacggta ttatcgttgt
      421 tgcttcaaac ccagtagata ttttgacata tatcgcttgg aacgaatctg gtctgccaac
      481 atcacgtgtt atcggtactg gaacaacatt ggatacaact cgtttccgta aagaaatcgc
      541 attgaaatta aaagttgacc cacgcagcgt ccacggctat atcttaggtg aacatggtga
      601 ttcagaagtt gctgcatggt cacatacaac tgtcggtggt aaaccagtgt ttgaaattgt
      661 agaaaaagac catcgtattg cccaagatga attagatgtg attgctgata aagtccgtaa
      721 tgctgcttat gagatcattg atcgtaaaaa agcaacttac tatggtattg gtatgagtac
      781 tgcacgtatc gtaaaagcta tcttgaataa cgaacaagct gtattgcctg tatctgctta
      841 tttaactggt gaatacgacg aaaaagatat ctttacaggt gtgccatcaa tcgttgacga
      901 aaatggtgtt cgtgaagttg ttgaattatc aatcaacgaa gaagaaaaag caatgttcag
      961 caaatcaaca agtgctttaa gagaagtatt gaacactgta ttataagaaa caatgaatat
     1021 gaactaaaaa tcctaaaact gcttaacagc tgaaatagct gttaagcagt tttttattga
     1081 tat
//
LOCUS       DAXWLF010000300         1076 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXWLF010000300 DAXWLF010000000
VERSION     DAXWLF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.306"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(61..285)
                     /locus_tag="ACGE4U_002781"
                     /pseudo
     CDS             complement(61..285)
                     /locus_tag="ACGE4U_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(839..925)
                     /locus_tag="ACGE4U_002782"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ACGE4U_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          373 a          185 c          156 g          362 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gctttt
//
LOCUS       DAXWLF010000301         1063 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.308, whole genome shotgun sequence.
ACCESSION   DAXWLF010000301 DAXWLF010000000
VERSION     DAXWLF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.308"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            3..884
                     /gene="galU"
                     /locus_tag="ACGE4U_002783"
     CDS             3..884
                     /gene="galU"
                     /locus_tag="ACGE4U_002783"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFF8394861.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          382 a          173 c          243 g          265 t
ORIGIN      
        1 aagtgaaggt caaaaaagca gtcattcctg ccgctgggct aggaacacgt tttttgccgg
       61 caacaaaagc catggcaaaa gaaatgcttc caatcgtgga taaaccaacg atccagttta
      121 ttgtggaaga agcattggat tcgggaatcg aagacatctt gatcgtaacg ggaaaagaaa
      181 aaagacccat tgaagatcac tttgatgcaa atatcgaatt ggaaaacaat ttgcgggaaa
      241 aggaaaaaac agaattactc gctttagttg aagaaacgac tcaagtcaat cttcacttca
      301 tccgtcaatc tcatccaaga ggactggggg atgccgtgct tcaagcaaaa gcatttatag
      361 gaaatgaacc gtttgtcgtc atgctggggg atgatttgat gaaagatgat atcccattga
      421 ctcaacaatt gatgatggat tatgaagaaa cacaagcttc cgctgttgcc gtcatgcgtg
      481 taccagaaaa tgaaacatct aaatacggaa ttattgatcc taaaacagat agcgaaaagg
      541 gtcgttgcag agtaaaaggg tttgtagaaa aacctgaaat gggaaaagca ccaagtaatt
      601 tagccattat cggacgttat ctactgactc ctaaaatatt tgagatatta gaaactcaag
      661 aaccaggagc aggaaacgag atacagctaa cggatgcact tcagactctt aaccagacag
      721 aggcggtcta tgcgagagag tttaaaggaa aaagatacga cgtaggcgat aagttaggat
      781 atatgaagac aaatattgaa tatggacttc aacattcgga aattgggact tcattaagtg
      841 catatattat ccagttgagt aaaaagctaa gaacagaaag ttaactggaa ataagtggcc
      901 tgctgagaat gaaaggctat ttgttattaa agcggcagct tgtgcgtcat ttgcgcaact
      961 gtcgttttag ttttttgact ctagatcagt tgagtcgtgg aaaacgaaag atagaaaaag
     1021 actttcgtga atagattcag aaaatattat gctaatggtt taa
//
LOCUS       DAXWLF010000302         1023 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXWLF010000302 DAXWLF010000000
VERSION     DAXWLF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.309"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(102..731)
                     /locus_tag="ACGE4U_002784"
     CDS             complement(102..731)
                     /locus_tag="ACGE4U_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFF8394862.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(724..>1023)
                     /locus_tag="ACGE4U_002785"
     CDS             complement(724..>1023)
                     /locus_tag="ACGE4U_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFF8394863.1"
                     /translation="IQFMPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIW
                     KPMHLQPVFDKYEYIGTDVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
BASE COUNT          315 a          203 c          132 g          373 t
ORIGIN      
        1 cattaaaata ttcgatactg ttttccgtca gaatttcctc taatatattc gttcgactga
       61 tataatcaaa aggatgaata agcagccact tttttttatt ctcactcatc tatcttcgca
      121 ccctcaaatt tctcagtagt cccacttgct actgatgtta ttccatcacg gacaataact
      181 ttctttagcg ttaaaaaaaa tatttttatg tctttcatca ttgaaatgtt ctccacatat
      241 tcaatatctt tttcaaattt ttctttccaa ctaattgcat tacgaccact gacttgagct
      301 aatccggtta aacctgggcg cacaaaatgt ctttttcgtt gttcattact gtatagtgaa
      361 agatactcaa ctaacagagg acgcggtcca ataatactca tatctccttt taaaatattc
      421 cataattctg gtagttcatc aagactagtc gaacgcaata ttttaccaaa ttgagtcaat
      481 ctgatatcgt ctgataataa attaccttct tcatctcttt gatctgtcat tgttcgaaat
      541 ttatacatca gaaaaatctt ttcatgtttt ccaggtcgtt gttgtttaaa gatcactggc
      601 gatccaagct ttattcgaac taggattgca acaaacgtca tcagtggcca taacattatt
      661 aaacctaaaa ttgaaaaaag aatatctatc aaacgtttaa agaacttttc gtagatacct
      721 ctattagaca ttttgcatca ctcgcttaat tgtattagtt actcgataca aatcatcatc
      781 ggtcatttta gaatctgaag gtaaacagat acctttttca aacaatgtct gagcaacatc
      841 tgtaccaata tactcatact tatcgaaaac tggctgtaaa tgcattggtt tccatatcgg
      901 gcgggcttct atattatttt tatctaactc atcaataaga tgattcgtat ttattccctt
      961 tactgttatg acacttaacc aataatttgg ttcattccaa ttattttctg gcatgaattg
     1021 tat
//
LOCUS       DAXWLF010000303          994 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXWLF010000303 DAXWLF010000000
VERSION     DAXWLF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.310"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            10..420
                     /locus_tag="ACGE4U_002786"
     CDS             10..420
                     /locus_tag="ACGE4U_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8394864.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            434..790
                     /locus_tag="ACGE4U_002787"
     CDS             434..790
                     /locus_tag="ACGE4U_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8394865.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          339 a          156 c          194 g          305 t
ORIGIN      
        1 agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga
       61 gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta
      121 acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt
      181 tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag
      241 caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat
      301 gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa
      361 attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag
      421 ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa
      481 ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg
      541 cgatctaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca
      601 gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg
      661 aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat
      721 tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt
      781 aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag
      841 tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata
      901 gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa
      961 tatattgaca tgtgtaaatt tacatgatat attc
//
LOCUS       DAXWLF010000304          991 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.311, whole genome shotgun sequence.
ACCESSION   DAXWLF010000304 DAXWLF010000000
VERSION     DAXWLF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.311"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..112
                     /locus_tag="ACGE4U_002788"
     CDS             <1..112
                     /locus_tag="ACGE4U_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8394866.1"
                     /translation="KKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            302..375
                     /locus_tag="ACGE4U_002789"
     tRNA            302..375
                     /locus_tag="ACGE4U_002789"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:336..338,aa:Ile,seq:cat)
BASE COUNT          321 a          168 c          176 g          326 t
ORIGIN      
        1 taagaaagtc tatgtaatga tgcgggcaac gaatgatcca gagggaaaca ctaaaggcgg
       61 tgcgcaagat ttccatgaca aacgttggta tttaaatatt cctaaacgat aaaaatagct
      121 cctcgttgag gagcagtaca taactatatt gacaactata aaaattattc gataaaatag
      181 tgatgttatc gcatatcttc actatcaccc ataaatagtc acactccaag ctatgcgata
      241 acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt ggcaacaacc
      301 agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc gtaggttcga
      361 gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat tttttgaaga
      421 gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt ttttatgtat
      481 ccttttatgg attaatgaaa ggatgtttca catagttata tttctgtata tttgaaaagt
      541 tttactttga tttttaaata gaaagacatt tgggttaaat tgtgagatga taataaagaa
      601 gagtttaaag cgcaccccaa gccacttccc cataagtgtg ttacgcttta aactctttta
      661 tatttgaagc tattaaaagg cataccatat ttttgaaaaa aagtgagaaa aaaggcttac
      721 aattggagtg gtagttaatt agtgacttat ttttgatttt atagcactga tactataaaa
      781 tatagatatc atcatattac acaatcttaa tactaactta aaaaatatct cctttcataa
      841 gtatggtgat aaaatccgtt ccgggctacc tttttaggta gcctatttta atctttgtat
      901 ctttctggat caacgaaagt atactttata tagtcataac gccgatgatc gcttcgtgcg
      961 tctggcacgt cagtcacgac atcaaacaaa a
//
LOCUS       DAXWLF010000305          983 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.312, whole genome shotgun sequence.
ACCESSION   DAXWLF010000305 DAXWLF010000000
VERSION     DAXWLF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.312"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..250)
                     /locus_tag="ACGE4U_002790"
     CDS             complement(<1..250)
                     /locus_tag="ACGE4U_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFF8394867.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITF"
     gene            complement(252..971)
                     /locus_tag="ACGE4U_002791"
     CDS             complement(252..971)
                     /locus_tag="ACGE4U_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8394868.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT          343 a          193 c          107 g          340 t
ORIGIN      
        1 taaaagtaat aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa
       61 tacctaaaat ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca
      121 taagagacat tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa
      181 ctacagatgt aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat
      241 tttgaagcat attattcccc ctctccaata aatatagtat ctccataggc tttttttaaa
      301 ttttcttcat taaatacaga ttctacactt ccgtgagcaa ttagatttcg attcaaaaga
      361 ataatattat caaaatactt ctttaccttg cttaaatcat gatgcacaat tagcactgtt
      421 tttccctgat gtttcaattt ttttaacgta tccataatta ttcgttcact aactaagtct
      481 atccctacaa atggttcatc taaaaatatg aaatccgcat tttgagcaag acatcttgct
      541 aaaagtacac gttgaaattg tcctccagat aattctccta tttgatgatt agaatatttt
      601 cccatatcga ccttttccaa agcctttgaa actctttgcc attctgcatt tttaattctt
      661 tgaaaaactt tcattccggc atatgttccc atagatacac attcttttac ttttattgga
      721 aaagtaaaat caatatcagc tttttgctct acatatgcaa tcttcaccag ttttttattg
      781 atatcttcat tattaatgga gacctctccc tgatgaggga taatatttaa cattgcttta
      841 agcaatgtgg attttccagc accatttgga ccaataatac ctgttaaagc accttcttgt
      901 atttttaaag aaatctcgtt caatgctaaa aaatcgttat aacaaacatt aagctttttc
      961 acttctaaca taactttctc ctt
//
LOCUS       DAXWLF010000306          978 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.313, whole genome shotgun sequence.
ACCESSION   DAXWLF010000306 DAXWLF010000000
VERSION     DAXWLF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.313"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            82..>978
                     /locus_tag="ACGE4U_002792"
     CDS             82..>978
                     /locus_tag="ACGE4U_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFF8394869.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYE"
BASE COUNT          333 a          182 c          192 g          271 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaa
//
LOCUS       DAXWLF010000307          966 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.314, whole genome shotgun sequence.
ACCESSION   DAXWLF010000307 DAXWLF010000000
VERSION     DAXWLF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.314"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(23..880)
                     /locus_tag="ACGE4U_002793"
     CDS             complement(23..880)
                     /locus_tag="ACGE4U_002793"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8394870.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
BASE COUNT          251 a          189 c          183 g          343 t
ORIGIN      
        1 aagactctgt tcttttttat ttctattccc ataatccagg taaagcttta ccgactattt
       61 tttcttgagt aaatggatgg atcatgtgca gttctaatgc atggagcatc agtcgttcag
      121 ctgttctatt ttggtagaga gggtctccaa cgatcggatg tcccttatga gctaaatgaa
      181 ctctgatctg gtgtgtacgt ccggtttcca aaacacaata gacttgtgtt tgttcagaaa
      241 gtactttgtt gacagtgaca tgtgtcaagg cgctctgacc atttttatca tctacgaccc
      301 tttttctccg atcatggcga tctcgtccaa ttttattggt gatggtcata tctttggcaa
      361 tttctcccca tacgatggct tgatatctcc gatagatcat tttttgttcc aacattcttc
      421 caagtatcgg caagacaaat ggattttttg caaataaaac ggcaccgctt gtttctttgt
      481 ccaagcggtg gacaacgtaa ggtcgttgct gtttagtgtc gagataagca gctaaatgat
      541 tgagtaaggt actgttttct gtcggttcat ttggatgagt cttcattcct gcaggcttat
      601 ccactataat caaatgctca tcttcatata aaacacggat gtgcttcgca tctcctaatt
      661 gaacttcttg ataattataa tctgtttctt cgaataacaa tgtgatctgg tcattgccct
      721 ttacttcatg atggaacaaa gcaggttgtt gattgatcca aacgttttta cgtatacgca
      781 aaaagtgtcg aacctttcta ggaactaacc attccttttc aagcaagtcg cgaattgttg
      841 tttcaggatg gttcataggt aaagtgatcg tgattttcac aagtgtactc ctttcagtgc
      901 aatcgcagac taattgtacc atttttaaaa catatttaag aaaaaactta catttttctg
      961 ttcatt
//
LOCUS       DAXWLF010000308          956 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.316, whole genome shotgun sequence.
ACCESSION   DAXWLF010000308 DAXWLF010000000
VERSION     DAXWLF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.316"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            73..>956
                     /locus_tag="ACGE4U_002794"
     CDS             73..>956
                     /locus_tag="ACGE4U_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8394871.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPR"
BASE COUNT          336 a          166 c          176 g          278 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      721 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      781 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      841 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      901 gtagatgaat tttttattca atctgtcgca tctaaacgaa ataatattcc tagaaa
//
LOCUS       DAXWLF010000309          946 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.317, whole genome shotgun sequence.
ACCESSION   DAXWLF010000309 DAXWLF010000000
VERSION     DAXWLF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.317"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..456
                     /locus_tag="ACGE4U_002795"
     CDS             <1..456
                     /locus_tag="ACGE4U_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFF8394872.1"
                     /translation="TTTHSYDDRLNTLKKIKAFDINICSGFICGMGETDTQLVDLAFE
                     LKEQEPFSIPVNFLLPIKGTKLEKYNELTPIKCLKILIMLRLLFPKTEIRISAGREFH
                     LGSLQQFAILIVDSIFLGNYLTEKGADVTEDYQLLKELELIVEGDYNEY"
     gene            446..>946
                     /gene="bioD"
                     /locus_tag="ACGE4U_002796"
     CDS             446..>946
                     /gene="bioD"
                     /locus_tag="ACGE4U_002796"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFF8394873.1"
                     /translation="MNISMVICGIDTNCGKSVASILIYEYLKLYFSVNPIIVKPVQTG
                     QELDTIFYQQCDIPQKNIFNFYSLDIEASLHTAGNLTNQKIDLQSLLDQSKALLSDNR
                     PKIFELAGGILSPINDKETMLDFVKLLNLPIILVVDNYLGSINHTLLTIHLLESKNIE
                     ILGLIYN"
BASE COUNT          354 a          134 c          137 g          321 t
ORIGIN      
        1 actactactc attcctatga cgatcgttta aatacgttaa aaaaaatcaa agcttttgac
       61 ataaatatct gctctggctt tatatgtgga atgggagaaa ctgatacaca attagtcgat
      121 ttagcttttg aattaaaaga acaagagcct ttttcaattc ctgtaaattt tttattaccc
      181 attaaaggaa ccaaacttga aaaatataat gaactaaccc caataaaatg tctaaaaatt
      241 ctaataatgc ttagactatt gtttccaaaa acagaaatta gaataagtgc tggtagagaa
      301 tttcatttag gcagtcttca gcaatttgct attttaattg tagattctat atttttagga
      361 aactatttaa cagaaaaagg cgcggatgtc acagaagatt atcaattatt aaaagagtta
      421 gaacttatcg tagaggggga ttataatgaa tattagtatg gtgatttgtg gaatagatac
      481 aaattgtggt aaatcagtag ccagtatttt aatttatgaa tatctgaaac tctatttttc
      541 agttaatcca attattgtaa agccagtgca aacaggacaa gagcttgata ctatttttta
      601 tcaacagtgt gatatacctc aaaaaaacat ttttaatttc tattcattag atatcgaagc
      661 ctcactacat actgcaggga atttaacaaa tcaaaaaatt gacttacaat ccttgttaga
      721 tcaatcaaaa gctttactaa gtgacaatcg acctaaaata tttgaattag ctggtggaat
      781 tttatctcct atcaacgata aagaaacaat gttagatttt gtcaaattat taaaccttcc
      841 aattatctta gttgtagata attacttagg ctcaattaat catactttac taactatcca
      901 tcttttagaa agcaagaata tagaaatttt gggattgatt tacaat
//
LOCUS       DAXWLF010000310          941 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.318, whole genome shotgun sequence.
ACCESSION   DAXWLF010000310 DAXWLF010000000
VERSION     DAXWLF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.318"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..243
                     /locus_tag="ACGE4U_002797"
     CDS             <1..243
                     /locus_tag="ACGE4U_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394874.1"
                     /translation="ILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHDVDRNEI
                     LEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(605..844)
                     /locus_tag="ACGE4U_002798"
     CDS             complement(605..844)
                     /locus_tag="ACGE4U_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394875.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          332 a          139 c          172 g          298 t
ORIGIN      
        1 attttaggaa aaaaatatat ttgccaacct attggcttga aacaaaaagt gattggagaa
       61 gtaatcagta aattagaaaa ttgtgttgtg atctgcgtag acgaatatca tgacgtcgat
      121 cgcaatgaaa tattagaaaa atgcggaaaa gtagtggcaa aatatactca cgtttatgag
      181 ttggaaacaa cagaatatct atataactat agcaacaaat atgaacatac tttagtttca
      241 taaagattta aataaagtgc cttcaaaagc gagaagacca agcttttgaa ggcactttat
      301 ttagttggca gtgattgttc atgatttttg atttattcaa aaggttcgcc tagagaatct
      361 gtctctgtaa cgaggcctgg tggagaaact cgataaaaac ctatactgcc agaactgtcc
      421 tattttcgga tagacaaact agtagtttta aaaagatcat ccgatccaat catttcaaaa
      481 aatgcaaaac tgatatcagg atcatttagc tcaggcttgt tatcgtaaag catttgtaaa
      541 ggttgcattt agttaggagt aaacgcgaaa ctatcatcat tatttgctga agaagatgaa
      601 gaagctaatt cagagtttgt ttgaaataaa ggagacgtgc tgacagtttc tttaccctgt
      661 tttttgcaga ggtagaaact tttatatcgg aagtttctac cggagaattt ttctcatttg
      721 tagaaattgt tgaatcgata ctattcgaaa tttgcgtatc tgaaggtatg ttctttgatg
      781 cagaagtccc acaagcagta agtaacaata aatttgataa actgataaac agaattcttt
      841 tcattatgta cacactcctt atagtatcgt ttagcataca cctatgctca gtattaggca
      901 aaatcaatgc aaaactatca agtcttttat agtatctatt a
//
LOCUS       DAXWLF010000311          937 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.319, whole genome shotgun sequence.
ACCESSION   DAXWLF010000311 DAXWLF010000000
VERSION     DAXWLF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.319"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            79..771
                     /locus_tag="ACGE4U_002799"
     CDS             79..771
                     /locus_tag="ACGE4U_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFF8394876.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          409 a          146 c          157 g          225 t
ORIGIN      
        1 aatttaaaaa cgcttgacat tgtgtttgag atcacttact tttagcatgt aaaagtattg
       61 gattaaggag ctatgcaaat gaaaaagaaa tttattactg gtgggattgt cgttgcattt
      121 ctatgtctaa ttggctacac aggcacgcgg tacgcaaatg aaattatttt gtggggtgga
      181 gagacagata ttgatacaat caatgagaat ctgacacagc ttgatcatac attgacagat
      241 aaagagcaag cgattacaga tcttaccgct cgactagcac aaaaagaaca gacattgcaa
      301 gattcaaagg cagatgtcca agaataccaa acaaaagtaa atgaattaga aaaccaaaaa
      361 aatcaatcgg cagcagaaaa acaaaactta gaaagtcaat tagtcagcaa ggatgcacaa
      421 ctacaaagca aacaaaatga aatcaatgtc aaactagatg agatcaacca aaaaaatgat
      481 gaaatcaatc atctgaatgc agattggaca gcaagactac aagaagcaca agcaaaactt
      541 tcagaattac aaaatactgt aaatggtttg aatcaacaaa tcgcaacact gacacaagaa
      601 aaaacaaatg tagaaactca gttaacagat acacaaaaga aattagacga agcaactgct
      661 gaaaactcaa gcttgaaaca atatattcaa aagttagaaa aagcaaaaga tgaagtggaa
      721 gatacagctg ataaatctca gcaaattgtt gaagaacatt tgcgtaaata atctttctta
      781 acaagtaaac ctaaattagt aaagaagcaa cagtaatcta tactaagtag ttcagttttg
      841 gagtacaaaa tgacaaaatg tacttcaaaa tggactactt ttttcatttt aacttacata
      901 ccaaattaca tacaaaatga tttataaaat gcaattg
//
LOCUS       DAXWLF010000312          926 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.320, whole genome shotgun sequence.
ACCESSION   DAXWLF010000312 DAXWLF010000000
VERSION     DAXWLF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.320"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..>926
                     /locus_tag="ACGE4U_002800"
     CDS             <1..>926
                     /locus_tag="ACGE4U_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8394877.1"
                     /translation="SWFFQGIEEFSKVVIRNTVIKLSSVLLIILFVSTKDDLWIYVFI
                     TSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLFMQVYTTLDKTIV
                     GYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKSENILVYTKKSFD
                     YTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVSPIGGVFANQFAI
                     AIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLVFFFRVFFVRKEI
                     KISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFS"
BASE COUNT          278 a          128 c          157 g          363 t
ORIGIN      
        1 tcttggtttt ttcaaggaat tgaagaattt tcgaaagtgg ttattagaaa tacagttatc
       61 aaattatcat cggtattgtt aataatcctt tttgtttcaa ctaaagatga tttgtggatt
      121 tatgtgttca ttacttcaat tactaacttt ttaggaagtt tagttttctg gttgagtatc
      181 cacggaaaag taggtaaacc actctttaac tctaatgttt ttaaacagtt gtggaaacca
      241 gcttttatta ttttgactcc tcagttgttt atgcaagtgt acacaacttt agacaaaacg
      301 atagttgggt attttgtgga tccaacagca ttatcttatt atgatcagtc tcaaaaaatt
      361 gctcgtatta tattggcagt cttaacatca attactattg ttatgttgcc taaaatatct
      421 aaagcacagg cagagggaaa atcagaaaat attttagttt acacaaaaaa atcttttgat
      481 tacacgatta ttattgcatt aattcttttt agtgtggtgt ttatcaatac aaaagaattt
      541 gttccatggt tttttgggga gaatttcaaa ccgatgacaa taaacatgct tatttcttgt
      601 ctaattatag ttgttagtcc aattggtgga gtttttgcta accaatttgc aattgctata
      661 gaaaaagata aggagtatgc ttatccaatg atcattggtg cgattgtaag tattattgga
      721 aatgtagctt ttgtgcctac ttatggcgct gtagcggcta caatcgtact cgtggtagta
      781 gaattattag tctttttctt tagagtattt tttgtacgaa aagaaataaa aatatcctat
      841 ttctttggga agaacgttgt cttatttttt atagtcaatc ttactttgac tacttcggcc
      901 tattactttc ttccacaatt attctc
//
LOCUS       DAXWLF010000313          923 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.321, whole genome shotgun sequence.
ACCESSION   DAXWLF010000313 DAXWLF010000000
VERSION     DAXWLF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.321"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..863
                     /locus_tag="ACGE4U_002801"
     CDS             <1..863
                     /locus_tag="ACGE4U_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFF8394878.1"
                     /translation="TSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNIL
                     ARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLA
                     FARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVIL
                     EKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQN
                     DFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMD
                     QSKKENQVTS"
BASE COUNT          340 a          159 c          147 g          277 t
ORIGIN      
        1 caacttctgt gaaagacgta caaaaagaat tcacagagta tgaatatatt gactttttta
       61 ttgatccctc tgtagatatt ttgcgaccta atctgccaat ttgtcgcgaa caacgcatct
      121 tagtgaatat tttagctcga ctttctatca cgaaaatcga atcggaagaa acaagcttgg
      181 agcaatacaa acttttaaaa aaagcaaacg ttcctcatgt tctttattca agagaaatgg
      241 tggagtcctt ttgtactgta tttgatttgt ttatacctga aaaagaaaag attctttact
      301 cattggcatt cgcacgaaat aaactttata atggatttct taacttcaat caaccaaata
      361 cgccccttcc tactttcatg ctttatcaag aacatagcgt gttgaaagaa gtccaaaccg
      421 ccattgaaca attttatatg gatttcagag aacaaaatca acacttgtta ccagttattt
      481 tagaaaagga aaatgtgcaa tggatggttg aagacctagt acatttgtat gatcgctaca
      541 aaaaacagcc gcgaattgtg attggtgtga actatacgcg tgatttttat atcagtaaag
      601 atctgatcgt taagatagaa caaatttttt caaatgaatc gatcaaaata caaaatgatt
      661 ttatggagaa ctgcaacatc gttatcagcg actgcccttt gaaacaattg ccttctcata
      721 tcaaaaaaat ctatatttta aaaggaatca tcacaccaga agactggaaa aaagtgatta
      781 tgaggatctc agaatatatc tttgaattaa aagaagaagc actcatggat caatcgaaaa
      841 aagaaaatca agttacttcc tagaccaaat aagatacata aatccgaatg cttattaaca
      901 acctccggat ttatgtatca aga
//
LOCUS       DAXWLF010000314          908 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.322, whole genome shotgun sequence.
ACCESSION   DAXWLF010000314 DAXWLF010000000
VERSION     DAXWLF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.322"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..>908
                     /locus_tag="ACGE4U_002802"
     CDS             <1..>908
                     /locus_tag="ACGE4U_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFF8394879.1"
                     /translation="EINQKYDETLKNVYSSLIVSNIEETSSSEIKNKLSLLKLKEKEY
                     IIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKVLLNKLTISNIDSTREKIIKIFDKI
                     NNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREID
                     REKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVL
                     QRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIF
                     SEDAPSLETKLHNHFRKQEVNKINQR"
BASE COUNT          396 a           99 c          145 g          268 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaagtattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaaga
//
LOCUS       DAXWLF010000315          904 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.323, whole genome shotgun sequence.
ACCESSION   DAXWLF010000315 DAXWLF010000000
VERSION     DAXWLF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.323"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            287..694
                     /locus_tag="ACGE4U_002803"
     CDS             287..694
                     /locus_tag="ACGE4U_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394880.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..>904
                     /locus_tag="ACGE4U_002804"
     CDS             708..>904
                     /locus_tag="ACGE4U_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394881.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDG"
BASE COUNT          338 a          117 c          159 g          290 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaa
//
LOCUS       DAXWLF010000316          901 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.324, whole genome shotgun sequence.
ACCESSION   DAXWLF010000316 DAXWLF010000000
VERSION     DAXWLF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.324"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            3..305
                     /locus_tag="ACGE4U_002805"
                     /pseudo
     CDS             3..305
                     /locus_tag="ACGE4U_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            413..>901
                     /locus_tag="ACGE4U_002806"
     CDS             413..>901
                     /locus_tag="ACGE4U_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFF8394882.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT          333 a          138 c          154 g          276 t
ORIGIN      
        1 aaatgaacaa acataataga aaaataacga ataacaaaga actgatcttg ttactaatag
       61 ggatttgggg tatcttttta ctctttaata tgtatatgcc gactatgaga gcggatgacg
      121 tagtttatgc aagtagatta gatgaacttg gctatttggg tgcttcgata gaacattata
      181 agacatggag ttcaagaatt attatagaac tattcttaat gttcttttct aaacacttta
      241 tgctatggaa attattaaat tctacgatta tgttaggaat tgtagtatta ctttgcaagt
      301 atgttgagag tataaaatat tttgtgtaaa tgaaaaaatc catacaaaaa aggaagtccc
      361 ttctgtagaa taaagttaac gacaaccaat tcacagaaaa gaggacttcc ctatgaatga
      421 ttttactaca gaaattgtgc aaactctagt cactaaaggc gatttaaatg aattattccg
      481 ttcgcactta gaaaaagcga taaacacact cctacggact gaattaacgg cttttttaga
      541 ttacgaaaaa tatgatcgca ctggttttaa ttcaggtaat tcgagaaacg gttcttactt
      601 tcgatcaatc aaaaccgaat atggtgaatt aacattggaa atacctagag atcgtaatgg
      661 tgagtttaaa caacaaactt taccagccta caaaagaaca aacgatacat tggaaaccac
      721 tattatccat ttattcgaaa aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa
      781 aatgtacggt catcactata ctccacaaac catgtccaac atgactaaag ttctgactga
      841 agaagtaaat gcctttaaat ccagagcctt aaatgataag tatgtcgcta tttttatgga
      901 c
//
LOCUS       DAXWLF010000317          888 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.325, whole genome shotgun sequence.
ACCESSION   DAXWLF010000317 DAXWLF010000000
VERSION     DAXWLF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.325"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..>888
                     /gene="bsh"
                     /locus_tag="ACGE4U_002807"
     CDS             <1..>888
                     /gene="bsh"
                     /locus_tag="ACGE4U_002807"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFF8394883.1"
                     /translation="TSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVNDLD
                     THYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPW
                     ILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYD
                     NPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSS
                     VSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCN
                     VDKGIYYYRTYEDSQITAI"
BASE COUNT          303 a          122 c          160 g          303 t
ORIGIN      
        1 tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg attatgaaat
       61 atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt ttcgaaaggt
      121 aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag ctgactaccc
      181 tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa atttttctgg
      241 gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt ttgaatttat
      301 tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt taaaaaatat
      361 caatttagca aatataaatt atagtgacga acttccttta tcccctttac attggctatt
      421 agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc atatatatga
      481 taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat ttaatttaaa
      541 caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc aaataagttt
      601 gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat cctcagtatc
      661 tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt cagagtcaga
      721 aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag gtttgtgtga
      781 tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg ttgacaaagg
      841 aatctattat tatcgaacat atgaagacag tcaaattact gcaattga
//
LOCUS       DAXWLF010000318          860 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.326, whole genome shotgun sequence.
ACCESSION   DAXWLF010000318 DAXWLF010000000
VERSION     DAXWLF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.326"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..103
                     /locus_tag="ACGE4U_002808"
     CDS             <1..103
                     /locus_tag="ACGE4U_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialic acid O-acetyltransferase NeuD family
                     sugar O-acyltransferase"
                     /protein_id="HFF8394884.1"
                     /translation="NMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            105..>860
                     /locus_tag="ACGE4U_002809"
     CDS             105..>860
                     /locus_tag="ACGE4U_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFF8394885.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNE"
BASE COUNT          305 a          120 c          191 g          244 t
ORIGIN      
        1 aaatatgaac attataggtg cgggaagtgt agtaataaaa aatattaata ataagactaa
       61 aaatgttggc gtacctacca aagtcataga aagatgggat taagttggaa aataaacgaa
      121 tattattagc atctcctcat atgagtgatg aaggatatga acaagagtat gtaaaagaag
      181 catttgatac aaattgggtg gctcctttgg gtatgaatgt caatggtttt gaagaggaat
      241 tagcagaata tgtcggtgct agtcatgcag ctgcactatc ttctggaaca gcagctatac
      301 atctagcttt aaaagcggct ggggtcggta aagatgatat agtgatttgt cagtctttga
      361 cgttctcagc tactgccaat ccaatcatct atcaaggagc tattccagta tttgttgata
      421 gtgattacga aacttggaat atgaaccctg aacatttgcg aacagcgcta gaaaaatatc
      481 ctaacgcaaa agctgtatta gtagtgcatt tgtacggttt atcagctgaa ctagatgaaa
      541 ttactaaaat atgcaaagaa cataatgttg ttttaattga agatgctgca gaatcattag
      601 gaacaacata taaaggaaaa tatactggta cattcggcga ctatggaatt tattctttta
      661 atggaaataa gataatcacg acttcaggtg gcggaatgct tgtgtctaat gacaaagaaa
      721 aggtggaaaa agtgaggttt tgggctacgc aatctcgtga gaaggcacgc cattatcaac
      781 acaatgaaat tggctataat tatcgaatga gcaatatcgt tgcaggtatc ggtcgaggac
      841 aattgaaagt tttaaatgaa
//
LOCUS       DAXWLF010000319          852 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.327, whole genome shotgun sequence.
ACCESSION   DAXWLF010000319 DAXWLF010000000
VERSION     DAXWLF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.327"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..135
                     /locus_tag="ACGE4U_002810"
     CDS             <1..135
                     /locus_tag="ACGE4U_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8394886.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            113..>852
                     /locus_tag="ACGE4U_002811"
     CDS             113..>852
                     /locus_tag="ACGE4U_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HFF8394887.1"
                     /translation="MESFVSLNLTNNTSKKNQPKDIKDIVDTRMYKERDDLNYTLIIN
                     KDGNYSWRPLVLLHPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASK
                     GSKRTDLGETILNWWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEK
                     VKNDENKCSGSLGDKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELK
                     KLENLKDYKVLRYRDDYRIFSNNRNDLE"
BASE COUNT          333 a          124 c          140 g          255 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatac ttcaaaaaaa aaccaaccga aagatataaa
      181 agatattgta gatacacgaa tgtataaaga acgtgatgac ttaaactata cgcttattat
      241 caataaagat ggaaactatt catggcgtcc tctcgttcta ctacatccta ttctatatgt
      301 agatttggtt aattgtctca ctaaagaaga ccattgggag gagttaaaaa agaggtttag
      361 tgaatttaaa aaagataaaa atattaactg ttattctata cctgttgaag cttccaaagg
      421 ttcaaaaagg acagatcttg gtgaaacaat actaaactgg tgggaatatt ttgaacaaaa
      481 aagcttagct cagaatatta aataccaata ttgcatgttt actgacatcg ctgattgtta
      541 tccatcaatt tatactcact ctataacatg ggccatgtat ggtaaagaaa aagttaaaaa
      601 tgatgaaaat aagtgttcgg gctcacttgg cgataaaata gataaaaaaa tatctggaat
      661 gcaacacaat caaacaaatg gtatccctca gggttccgtt cttatggatt ttgtagcaga
      721 gatcgttcta gggtatgttg atttgagatt atcgaaagaa ttaaaaaaat tagaaaattt
      781 aaaagattac aaagtactca gatatagaga tgattataga atattttcaa ataatcgtaa
      841 tgatttggaa ca
//
LOCUS       DAXWLF010000320          828 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.328, whole genome shotgun sequence.
ACCESSION   DAXWLF010000320 DAXWLF010000000
VERSION     DAXWLF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.328"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(15..87)
                     /locus_tag="ACGE4U_002812"
     tRNA            complement(15..87)
                     /locus_tag="ACGE4U_002812"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:His,seq:gtg)
     gene            complement(90..163)
                     /locus_tag="ACGE4U_002813"
     tRNA            complement(90..163)
                     /locus_tag="ACGE4U_002813"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(129..131),aa:Trp,seq:cca)
     gene            complement(170..250)
                     /locus_tag="ACGE4U_002814"
     tRNA            complement(170..250)
                     /locus_tag="ACGE4U_002814"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(214..216),aa:Tyr,seq:gta)
     gene            complement(252..327)
                     /locus_tag="ACGE4U_002815"
     tRNA            complement(252..327)
                     /locus_tag="ACGE4U_002815"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(292..294),aa:Phe,seq:gaa)
     gene            complement(363..435)
                     /locus_tag="ACGE4U_002816"
     tRNA            complement(363..435)
                     /locus_tag="ACGE4U_002816"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(400..402),aa:Asp,seq:gtc)
     gene            complement(445..517)
                     /locus_tag="ACGE4U_002817"
     tRNA            complement(445..517)
                     /locus_tag="ACGE4U_002817"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
     gene            complement(533..604)
                     /locus_tag="ACGE4U_002818"
     tRNA            complement(533..604)
                     /locus_tag="ACGE4U_002818"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(569..571),aa:Glu,seq:ttc)
     gene            complement(619..708)
                     /locus_tag="ACGE4U_002819"
     tRNA            complement(619..708)
                     /locus_tag="ACGE4U_002819"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(672..674),aa:Ser,seq:gga)
     gene            complement(748..821)
                     /locus_tag="ACGE4U_002820"
     tRNA            complement(748..821)
                     /locus_tag="ACGE4U_002820"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(785..787),aa:Asn,seq:gtt)
BASE COUNT          234 a          226 c          189 g          179 t
ORIGIN      
        1 ccaagaataa aaagggcgaa tgatgggaat cgaacccacg aatgccggag ccacaatccg
       61 gtgcgttaac cacttcgcca cattcgccat ggcaggggca gcaggaattg aacccacact
      121 aacggttttg gagaccgctg ttctaccttt aaactatgcc cctataaaat ggaggagagt
      181 ggattcgaac cactgaaccc taaggaacgg atttacagtc cgtcgcgttt agccacttcg
      241 ctactcctcc atggtggcgc gggacagaat cgaactgccg acacacggag cttcaatccg
      301 ttgctctacc aactgagcta ccgcgccata ttttaaaatt attaattgaa taacaagaac
      361 gacggttccg acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc
      421 ctacaccacg gaaccagttc attatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccaac attcaattaa aatgacccgt
      541 acgggattcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg
      601 ggccaggtaa atttgctacg gagagtaagg gattcgaacc cttgagacag tttggcacca
      661 tctacatgat ttccaatcat gctccttcgg cctctcggac aactctccag agtgaaagaa
      721 gctcaatact ctttctcaaa atgagaactc cggcagtagg actcgaacct acgacatcat
      781 gattaacagt catgcgctac taccaactga gctatgccgg aataacat
//
LOCUS       DAXWLF010000321          826 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.329, whole genome shotgun sequence.
ACCESSION   DAXWLF010000321 DAXWLF010000000
VERSION     DAXWLF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.329"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            25..558
                     /locus_tag="ACGE4U_002821"
     CDS             25..558
                     /locus_tag="ACGE4U_002821"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFF8394888.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          262 a          160 c          160 g          244 t
ORIGIN      
        1 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
       61 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
      121 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
      181 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
      241 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
      301 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
      361 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
      421 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
      481 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
      541 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
      601 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
      661 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
      721 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
      781 ggttggactt attaccgcag atgcaagctt ttggacaccg tttacc
//
LOCUS       DAXWLF010000322          814 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.330, whole genome shotgun sequence.
ACCESSION   DAXWLF010000322 DAXWLF010000000
VERSION     DAXWLF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.330"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..675
                     /locus_tag="ACGE4U_002822"
     CDS             <1..675
                     /locus_tag="ACGE4U_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFF8394889.1"
                     /translation="SSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKID
                     NNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRT
                     DFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGR
                     KIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRL
                     HSMGLA"
BASE COUNT          298 a          127 c          152 g          237 t
ORIGIN      
        1 tctagcggta aatactatat ttctttgttg tgtaaagaag aaattatcga attacctaaa
       61 actaattcca caattggtat tgacttaggt attatggact ttgcgattct ttctgatgga
      121 caaaaaattg ataataataa attcacgtcc aaaatggaaa agaaactaaa gcgtgaacaa
      181 cgcaaattgt ctaggcgtgc tttattggct aaaaataaag gtaccaatct atttgaagct
      241 aaaaattatc aaaaacaaaa acgcaaagta gctagattgc atgaaaaagt aatgaatcaa
      301 cgtactgatt ttctgaataa gttgagtaca gaaatgatca aaaatcacga tattatctgt
      361 attgaagact taaacacaaa aggtatgttg cgaaatcata aattagctaa aagtatttct
      421 gatgtttctt ggtcaaagtt tgtgactaaa ttacaataca aggctgactg gtatggacga
      481 aaaatcatca aagtagataa atggtttccg tctagtcaga tttgttcaga gtgcggacat
      541 aaagatggca agaaatctct tgaaattcga gaatggactt gtcctgtttg tcatactcat
      601 catgatcgtg atatcaatgc tagtatcaat attctgaccg aaggtctaag actacattct
      661 atgggattgg cttagatcca atcatcaaga accgtaggaa ctacggggat agcttggtaa
      721 ataagagaca ccgctgttag taaagaaata cgctatcaag tatgctctat tcccaagaag
      781 ctcccacttc aagcgttaag aaccgtcagg ttta
//
LOCUS       DAXWLF010000323          807 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.331, whole genome shotgun sequence.
ACCESSION   DAXWLF010000323 DAXWLF010000000
VERSION     DAXWLF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.331"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            3..>807
                     /locus_tag="ACGE4U_002823"
     CDS             3..>807
                     /locus_tag="ACGE4U_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8394890.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYT"
BASE COUNT          308 a          106 c          147 g          246 t
ORIGIN      
        1 gagtgcataa aattagtgtt atagtacccg catataacgt agaaaactat ttagaaaact
       61 gcttagaatc tatttccaat cagacacatt caaatattga aataatagta gtcgatgatg
      121 gttccccgga tcgaagtggg gaaattgcag aagaatataa aaagaaagat atgcgattaa
      181 aagtgataca tcaagtgaat ggtggtttaa gtgcggctag gaattctggt cttgattctg
      241 caacaggaga ttttgtgtgc tttattgaca gcgatgattg gatagaacca gactatcttg
      301 agttactata ttttggtacg caaacttatg gagcagatat ctcggtgcta aaaataaaaa
      361 agatttcaag tttagaacaa ctaaaagaac atacaattgc tgagagaaaa tgggaaatat
      421 ttgatagaga acaagcaatg aaaaacttat ttataaataa taaaattggc tattctgcaa
      481 ataataaatt attcaataga aatttatttg ataatataag attcccactg ggtaaattaa
      541 tggaagataa ggctactact tatttgttga tcgatcattc cgaaaaagtt gctgtcaatt
      601 catctgctaa atatcactat taccaaagcc ctaaaagtat actgagagga agcttcaatc
      661 ctagaatttt tgattctttt gaagtccatg aagatatact agtattcata gataagtttt
      721 atccggaatt atcaattaat gttagaactc gctatgtcta tgaagcaata aggatgatga
      781 tgacaatgat taaaaataac tatacca
//
LOCUS       DAXWLF010000324          793 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.332, whole genome shotgun sequence.
ACCESSION   DAXWLF010000324 DAXWLF010000000
VERSION     DAXWLF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.332"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..782
                     /locus_tag="ACGE4U_002824"
     CDS             <1..782
                     /locus_tag="ACGE4U_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8394891.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          244 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc ttt
//
LOCUS       DAXWLF010000325          746 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.333, whole genome shotgun sequence.
ACCESSION   DAXWLF010000325 DAXWLF010000000
VERSION     DAXWLF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.333"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            116..715
                     /locus_tag="ACGE4U_002825"
     CDS             116..715
                     /locus_tag="ACGE4U_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8394892.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT          288 a          107 c          158 g          193 t
ORIGIN      
        1 tcataaacaa ggaattgcta gcttgatact ttctcaatag atagttataa taaacctcag
       61 aataaatttg aactatatat attataaaag tttaaaatta cgggaggctc tacaaatgaa
      121 cggttttgaa aagagagcac aagagaaaaa gaaagagatt ttacaagcag cttttgactt
      181 gatgaacaca gacataggaa ccgctggagt gacaatggat aaggtagctg agaacgctca
      241 ggtaagtaaa gcgacgattt ttaaatattt tggcagcaag gaagggctga tttatgaagt
      301 attttttgca tttatgaatg atttagggca agaagcgaaa gcgttgattg cggaaaatct
      361 tccatttgaa gaaacactga ttgccatgag tgaaaacaaa atccgttatc tcaatcgagt
      421 gaacaaacaa ttttatatcg atttgatgac atactataca aaaaaagaaa aaaaagaatt
      481 ttctgacgcg atggatgcgt atacgcaaga aagtttcagt atcatgcttg atcttttcca
      541 tcgagggcgg aaagaaggaa aagtggattt gaaatattcg gatgagttct tgatcctcta
      601 ttttcaagca atggtagaag gaatttctaa gccggagata tacgcaaaac tgcttccata
      661 tacagcagaa tggacagaag tcctgatcaa gggaatcgca ccgaagaaga aataaagcat
      721 aaaaaagccg aaactgacga taaaat
//
LOCUS       DAXWLF010000326          733 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.334, whole genome shotgun sequence.
ACCESSION   DAXWLF010000326 DAXWLF010000000
VERSION     DAXWLF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.334"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(91..666)
                     /locus_tag="ACGE4U_002826"
     CDS             complement(91..666)
                     /locus_tag="ACGE4U_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFF8394893.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          243 a          155 c          130 g          205 t
ORIGIN      
        1 tggaaagttt tgtctcgctt aatttgacaa ataataaaat gaaaaaacag agccagatta
       61 gcattatttt gctaatccag ctctgtttat tcagcctatt tttctttttg aatatatcaa
      121 gcgttggggc aacatagtga tgactaatga aacaacgacg atagacaaca agcgatcaag
      181 gtagtctgta cccgcctgta ccaagatcac acttgttgtc tgacttaaac ctaacccatg
      241 taatagctga acgatcatac tggatcctga ggaggtgatc ccgccaaata actgaacggt
      301 aatgatcgaa gaaacgatcg ttccaggtac cgacaaaatc aaagcgagta ataacagttt
      361 caagctgttt ttatctttgc cgagatatct tggcaaaatc aatccagcaa gcaagcctgt
      421 gagaatttgg attggcgaat aatacagaga aaaaatatct gtagtcactc cacttaaaat
      481 accgctggac aatcctgtca gcattccagg tacaggccca agcaatgcac cagcaaaaat
      541 cgttccgata gaatcgagat agattggcaa acgaagccat aaagcaatcg ttcctccaat
      601 ataattgatc ccaatacaca aagccatcac tgtaagtacg cgagttgcca ttttgttttt
      661 attcattttg ttagcccatc tttaaccttt ccaaatcttt cataatcgtt tcttcttgtg
      721 catttaaaag aac
//
LOCUS       DAXWLF010000327          726 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.335, whole genome shotgun sequence.
ACCESSION   DAXWLF010000327 DAXWLF010000000
VERSION     DAXWLF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.335"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(204..353)
                     /locus_tag="ACGE4U_002827"
     CDS             complement(204..353)
                     /locus_tag="ACGE4U_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394894.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          254 a          115 c          106 g          251 t
ORIGIN      
        1 tcgaaacagc taacaaaatt gtcataaaaa acgagatggt tgatattaga acttgtgttt
       61 ttgtttaggt ctagagataa agagagacaa tttccaaata tatgtaatga atacaaaaag
      121 acgtttgtcg cggattaccg ttacaaacgt cttttattga ttttccgaga tctcatcagt
      181 cgccatttgg aatggttcac tccttatcta gattcgttta atgctcggac tgcttgttcg
      241 attgtaatac caaacgcaac gttattttcg ttttgtgcat caatcgccat aaaacggtta
      301 gaagttgtat caaaagctac gcggtattgc atatttgttt ttgtaaacgt cataaaattc
      361 atctcctttg taatgtaatt tagttcttga aagtttcctt taaactttcg aacttatacc
      421 aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt tccttattta
      481 ttttttctta aatggtttag ctattttctt ttaatccgat aagtagccca atcatcaagg
      541 ttacaaatac tattgcttga atataaatca tatgctcacc tctttataaa taacttagca
      601 atataaagaa aaaaacgcac gaacaacata ggaatataaa caaacgtttt tgtcacaacg
      661 tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa agatactcaa aataaaaaat
      721 aaaaaa
//
LOCUS       DAXWLF010000328          704 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.336, whole genome shotgun sequence.
ACCESSION   DAXWLF010000328 DAXWLF010000000
VERSION     DAXWLF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.336"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(102..686)
                     /locus_tag="ACGE4U_002828"
     CDS             complement(102..686)
                     /locus_tag="ACGE4U_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8394895.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          221 a          162 c          101 g          220 t
ORIGIN      
        1 catttgagaa taagttatca gtagacactt acaaaaaatt ctcatactat tgtcactttt
       61 ctagataaat ggcaaaaatt ttcagcatta aatgacaaaa actaatgaga atttgtcttt
      121 ccatttttct cctccatttt tgccaaataa cgataaatgg tggttcttga gacgttccac
      181 cgttccgcaa tagtcttaat aggtgtaccg ctatccacta aagtcttcag taattccaaa
      241 tcttgttcag acaatttttc aggacgacca ccaaaccgtc ctcttgcacg agcagctgct
      301 cgtcctgctg cggatcgttc caggatcaaa ttacgctcaa attctgcaaa tgccgcaaac
      361 aaatgaaaca tgagctgtcc tgtagaactt gatttgtcca tcgtaatatt ttcttgaata
      421 ctatgaaaac tcacaccacg ctcattcagt cgattgacga ttgaaatcaa atcttccata
      481 tttctaccta aacgatctaa gcgccaaacg acaagtgtat cgccagagcg aacaaaatca
      541 atggccattt ctaatcctgg tcgattactt tttgccccac tcatatgatc agtaaaaatt
      601 ttttcacaac caaatttatt taggctgtct tcttgtaaat ccaaattttg taaaccagta
      661 gatacccggg cgtatcctat tttcaaaaaa atcactcctt catt
//
LOCUS       DAXWLF010000329          694 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.337, whole genome shotgun sequence.
ACCESSION   DAXWLF010000329 DAXWLF010000000
VERSION     DAXWLF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.337"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..338
                     /locus_tag="ACGE4U_002829"
     CDS             <1..338
                     /locus_tag="ACGE4U_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394896.1"
                     /translation="TNEPTTVVDNNQELTPLDRNGIAIGNPTNDLTVTENYLIVSPLL
                     ARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGKLYLQSYKSAGNNWIA
                     TYSGTLTRK"
     gene            348..551
                     /locus_tag="ACGE4U_002830"
     CDS             348..551
                     /locus_tag="ACGE4U_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394897.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
BASE COUNT          275 a          108 c          104 g          207 t
ORIGIN      
        1 gtacaaatga accaactact gtagtagata ataaccaaga attaactcct ttagatagga
       61 acggaatagc cattggtaac ccaactaacg acttaactgt tactgaaaat tatttaatag
      121 tttctccatt acttgctcga gcagcttcta aaacgatacg agtgacagga acgatgattt
      181 ttgataaagt tggttggcct tctagacctc ctcaatcacg gaattggaaa gaaaaaagaa
      241 atggttattg gtactatgga aaattgtatt tacaaagtta taaatcagct ggaaacaatt
      301 ggattgcgac ttattcagga accttaacta gaaagtaggg attttttatg ccagaatttg
      361 tgaacacacc aaatttttat caagatagcg atattctaag taaaattaat ttaccgaaag
      421 aagaatcagc tttacaagtt cagcgaactt tacaggagac acaaaaaatt caaaaatcaa
      481 tttctaatct agaagattct gaagaaaaaa gaaaaaaaag agaccaaaaa acatatcaaa
      541 aagaaaaata atgattttaa aaaaagagtg tctagtcgac attctttttt tctaattgaa
      601 agtgttcata aaatcaccca tttctggaca ctttcttttt ttgctttatt tttcaaaaaa
      661 acaattcatt tcatgttcat aaacccataa caat
//
LOCUS       DAXWLF010000330          656 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.338, whole genome shotgun sequence.
ACCESSION   DAXWLF010000330 DAXWLF010000000
VERSION     DAXWLF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.338"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            67..>656
                     /locus_tag="ACGE4U_002831"
     CDS             67..>656
                     /locus_tag="ACGE4U_002831"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394898.1"
                     /translation="MEGNSWGNYGLVFTAIFFAVIIYFFLNWAKTSLTSKVIVIVGAF
                     FMLLSSKLLPWNSIPHMFKFVSFFQFPQRFSVIAFVLLLLSFALILQESKLLKDVDKK
                     YYILTLLCALFSIFNVYNLMYDQSWHWNTNDPTAAGNNKSSMVEKDPQKLREAFYNKD
                     LNIALKAIQKGTPDYLPVQKNVEPSDVLKQNPYELYT"
BASE COUNT          218 a          107 c           91 g          240 t
ORIGIN      
        1 ctaagtaata aaatattatc cccttctccg ataagtcaaa tgttgagcca aagtatgagt
       61 ttctcgttgg aaggaaactc ttggggaaat tatggtctag tatttactgc aattttcttt
      121 gctgtaataa tatatttctt tttaaattgg gctaaaacta gtcttacttc aaaagtaatc
      181 gttattgttg gagcgttttt tatgttactt tcttccaaat tattaccttg gaactctatc
      241 cctcatatgt ttaaatttgt ttcatttttc caatttccac aaagattctc agtcattgct
      301 tttgttttac ttttattatc atttgctctt atattgcaag aatcaaaact acttaaagat
      361 gttgacaaaa agtactatat attaacttta ctgtgtgctc tattttcaat atttaatgta
      421 tataatctta tgtatgacca atcttggcac tggaatacaa atgatcccac cgctgcagga
      481 aacaataaaa gttctatggt tgaaaaagat cctcaaaagt taagggaagc cttttataat
      541 aaagatctga atattgcctt gaaagcaatc caaaaaggaa ctcctgacta tttacctgta
      601 caaaaaaatg tagaaccatc agatgtatta aaacaaaacc cttatgaatt gtatac
//
LOCUS       DAXWLF010000331          644 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.339, whole genome shotgun sequence.
ACCESSION   DAXWLF010000331 DAXWLF010000000
VERSION     DAXWLF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.339"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(128..>644)
                     /locus_tag="ACGE4U_002832"
     CDS             complement(128..>644)
                     /locus_tag="ACGE4U_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFF8394899.1"
                     /translation="IVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKE
                     AFGRIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYK
                     SVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDN
                     ERGSDTSGEAA"
BASE COUNT          188 a          147 c          118 g          191 t
ORIGIN      
        1 gaatggcaac actttttttg acaaaaatta taaggtgttg tgaatgctgc ggtgttatgg
       61 ctacgattgc ccttgacaat gagcatcctc ggatctgatt ttctccggca gaagcacaat
      121 cattcaatta cgctgcctct ccagaggtat cagatccgcg ctcattatca aggattcgct
      181 tcgccaatct ctgttgtcga atttggatcg gtgtttcata cgccaacgtg ccatgtaagc
      241 gtagatagtt ccaccaatgc acataatcaa acaattctag acgtagctgt gccagtgtct
      301 caaattggta ttgatgcacg aattctactt tgacagattt ataggttgat tccacaacgg
      361 cattatcata cggacaaccc ttcctactca atgaacgagt aattccaaaa ccattcagaa
      421 tctcatgaat ggtttggtta tcaaattcct ttccccggtc tgtatgaaaa agcttgacgt
      481 ctgttaaaga atacggtatc cgtccaaagg cttcttttac caatgaggca tctttcttct
      541 caccacagga ataaccaata atttctcgat taaacaaatc aagtattaag cagatataat
      601 gccacttttt ccccacgcga acataagtaa gatccgtaac gatg
//
LOCUS       DAXWLF010000332          626 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.340, whole genome shotgun sequence.
ACCESSION   DAXWLF010000332 DAXWLF010000000
VERSION     DAXWLF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.340"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..>626)
                     /locus_tag="ACGE4U_002833"
     rRNA            complement(<1..>626)
                     /locus_tag="ACGE4U_002833"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          126 a          201 c          136 g          163 t
ORIGIN      
        1 tcaaccttgc ggtcgtactc cccaggcgga gtgcttaatg cgttagctgc agcactgaag
       61 ggcggaaacc ctccaacact tagcactcat cgtttacggc gtggactacc agggtatcta
      121 atcctgtttg ctccccacgc tttcgagcct cagcgtcagt tacagaccag agagccgcct
      181 tcgccactgg tgttcctcca tatatctacg catttcaccg ctacacatgg aattccactc
      241 tcctcttctg cactcaagtc tcccagtttc caatgaccct ccccggttga gccgggggct
      301 ttcacatcag acttaagaaa ccgcctgcgc tcgctttacg cccaataaat ccggacaacg
      361 cttgccacct acgtattacc gcggctgctg gcacgtagtt agccgtggct ttctggttag
      421 ataccgtcaa gggatgaaca gttactctca tccttgttct tctctaacaa cagagtttta
      481 cgatccgaaa accttcttca ctcacgcggc gttgctcggt cagactttcg tccattgccg
      541 aagattccct actgctgcct cccgtaggag tttgggccgt gtctcagtcc caatgtggcc
      601 gatcaccctc tcaggtcggc tatgca
//
LOCUS       DAXWLF010000333          622 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.341, whole genome shotgun sequence.
ACCESSION   DAXWLF010000333 DAXWLF010000000
VERSION     DAXWLF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.341"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            74..442
                     /locus_tag="ACGE4U_002834"
     CDS             74..442
                     /locus_tag="ACGE4U_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFF8394900.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          219 a          104 c           99 g          200 t
ORIGIN      
        1 ttgttcgttt tttatacata actattgttt ttctggtaaa ataaatcaaa ccacttaatg
       61 gaggcgtttt agaatggaaa aatttcagcc gatcatcaat cagatcaaag aaaaagaaca
      121 tcaacaaaag atcaaagacc tttttgctaa gataacagaa acgtatcccc agctagacac
      181 aactgtaaaa tggaaccaac ctatgtttac tgaccatggt acctttatta ttgcatttag
      241 ttttgctaaa aatcattttt ctatcgcacc ggaaaaagca gcgattcgtg cccttgaaaa
      301 aaatatccaa gaagcaggct acgtatatac ggataacgtc atcaaagttc cttggaaaag
      361 tgtaatcaac tgggaattga ttgaacaatt gatttcattt aatattgaag ataaaaaagg
      421 tcatgataaa ttttggcgat aaagctttat acgaataaac cttttactgg cgaggaaact
      481 attcatcagc ttatacaggt tttgttcaat cctatccttt ttatcatccc tggttaaaca
      541 cttaattgac aaagtatctg ctatttttat aaggagtctt tcttgtgctg aaaaaaatca
      601 ctgattgaat ggctttgaat tt
//
LOCUS       DAXWLF010000334          616 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.342, whole genome shotgun sequence.
ACCESSION   DAXWLF010000334 DAXWLF010000000
VERSION     DAXWLF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.342"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(83..454)
                     /locus_tag="ACGE4U_002835"
     CDS             complement(83..454)
                     /locus_tag="ACGE4U_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394901.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          209 a          103 c           72 g          232 t
ORIGIN      
        1 actatataaa gtatactttc gttgatccag aaagatacaa agattaaata aaaaaataac
       61 aaacaaactc gcttgaaatt cattaggtga gtttgtttat tattttttct gcttctcgat
      121 tcatcagcgc catatctgtt ttcgtaatgg tttcattagg aatatagtca gcacattttc
      181 ttttctctaa cattctatac ccactattat gtattcctaa ggtattcaat tccttaactg
      241 ttgaaaacaa attccaaacg gtttggtggc ttccttttcc gctacttcca ttataaataa
      301 tatcataatt ttctttcaaa tactcatcag attttttaaa agcactgtag tatgctcttg
      361 aaacagcagt tcttctataa gattctttag aattatattt atcatctgat tcaatttgtt
      421 tggaaataga taaatattca ttaaagtcaa acattttatt cccccaaagt tgctactaca
      481 gatttcccca tatatttata gtcagataat acattatcga gagtatccaa aacatcgtaa
      541 tctgaggaaa acaattcaga tgttgttttg ataacaaaga aatacaagtt taaatcttca
      601 tcatatttta tattga
//
LOCUS       DAXWLF010000335          611 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.343, whole genome shotgun sequence.
ACCESSION   DAXWLF010000335 DAXWLF010000000
VERSION     DAXWLF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.343"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(145..459)
                     /locus_tag="ACGE4U_002836"
     CDS             complement(145..459)
                     /locus_tag="ACGE4U_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394902.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          242 a           98 c           72 g          199 t
ORIGIN      
        1 tataaactct agagaaatta attcatccta acataaaata attgttcact caaaagtttt
       61 tctagaacaa ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt
      121 cctgtactaa aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag
      181 tagttgtgta ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga
      241 aacgtctata acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc
      301 tccgtaaaaa atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt
      361 tctaaaacct atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa
      421 tattgctatt attaagataa aattactgta aaaatccaaa agctatcctc ctagcaacta
      481 tttaaatacc caaaaaaatt acctctagat aatatcatac ctacgttttc acttataaat
      541 tttttcataa aaaaagcgtt tagaggcata atcgttaata cagatggtaa ccaatgtatc
      601 tggtttgaac t
//
LOCUS       DAXWLF010000336          581 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.345, whole genome shotgun sequence.
ACCESSION   DAXWLF010000336 DAXWLF010000000
VERSION     DAXWLF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.345"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            78..>581
                     /locus_tag="ACGE4U_002837"
     CDS             78..>581
                     /locus_tag="ACGE4U_002837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394903.1"
                     /translation="MFNQKKEKQFKYAIKKKKGGGGAASFVIGAVFLGIGLISYPTLS
                     FASETTEINQSSEIQSSLTNSVLKESNSEEKQNIDMSEDSSINNDLELDSKATVSGSE
                     NSFDETTNNLETTNLSEDSIEVQSESIIQNEDSTDNDAVQELEMNPHEDNGDDISVEN
                     QMTEATEE"
BASE COUNT          225 a           74 c          105 g          177 t
ORIGIN      
        1 tttctttgtt ttcataaaat gaacgattat ttcgtctctt tttgattttt atactgtaat
       61 aaataaggag gaaaattatg tttaatcaaa agaaagagaa gcagttcaaa tatgcaatca
      121 aaaagaaaaa aggtggcggg ggagcagctt cctttgtcat aggtgcagta tttcttggaa
      181 tagggttaat ttcttatcct acattatctt ttgctagtga aacaacagag ataaatcaat
      241 caagtgaaat ccaatctagt ttgactaatt ctgttctaaa agaaagtaat tcagaagaaa
      301 aacaaaatat tgatatgtcg gaagatagtt ctataaataa cgatctagaa ctagattcga
      361 aagcaaccgt ttctggatca gaaaattcat ttgatgaaac tactaacaat ctagaaacta
      421 caaatttatc tgaagatagt attgaagttc agtcagagag tattattcaa aatgaagact
      481 cgactgataa tgatgctgtt caagaattag aaatgaatcc tcatgaagat aacggtgatg
      541 atatttcagt tgagaatcag atgacagaag ctacagaaga a
//
LOCUS       DAXWLF010000337          571 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.346, whole genome shotgun sequence.
ACCESSION   DAXWLF010000337 DAXWLF010000000
VERSION     DAXWLF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.346"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..>571)
                     /locus_tag="ACGE4U_002838"
     CDS             complement(<1..>571)
                     /locus_tag="ACGE4U_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8394904.1"
                     /translation="HIRASLKDAVEEGLIPFDPTRKAVIKGKDSLKPKEDKYLDYDQF
                     KSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDIDFEDQIITINKTWNYK
                     LNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIKNPHNFVFFNLKNGLV
                     SSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
BASE COUNT          165 a           94 c           83 g          229 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg t
//
LOCUS       DAXWLF010000338          568 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.347, whole genome shotgun sequence.
ACCESSION   DAXWLF010000338 DAXWLF010000000
VERSION     DAXWLF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.347"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..>568
                     /locus_tag="ACGE4U_002839"
     CDS             <1..>568
                     /locus_tag="ACGE4U_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFF8394905.1"
                     /translation="KGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVRE
                     IRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKL
                     AKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYK
                     EVPKHQHKRALVLTARKFTRLVDTLLRKH"
BASE COUNT          212 a          109 c          103 g          144 t
ORIGIN      
        1 aaaggaaaag gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt
       61 accatgagtt accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt
      121 ctcgtacgag aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa
      181 caagtaattg ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa
      241 gtctatgctg ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca
      301 aaattggcaa aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg
      361 caaaatacac ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct
      421 gccaactctg taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag
      481 gaggttccaa agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt
      541 ctggtggata cgctactacg taaacacc
//
LOCUS       DAXWLF010000339          566 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.348, whole genome shotgun sequence.
ACCESSION   DAXWLF010000339 DAXWLF010000000
VERSION     DAXWLF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.348"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..461)
                     /locus_tag="ACGE4U_002840"
     CDS             complement(<1..461)
                     /locus_tag="ACGE4U_002840"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFF8394906.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQ"
BASE COUNT          155 a          128 c           94 g          189 t
ORIGIN      
        1 ttttgcagat atccttctaa caacaacaat tggcgtttga tttcaacccc atctattgcg
       61 tttcccatat atcctgtcag ttttccattt tgcccgatca ctcgatgaca aggaataacg
      121 attggcaatg gattggttcg atttgcctgc ccaatcgctc gcacagcttt cggactatca
      181 acagcaacgg ctacttcttt atacgttcgg atttctccat aaggaatggt cgacaaagct
      241 ttccagacat tttgctgaaa aggcgtacct ctctttatag ataaaggaac atcaaattct
      301 tttctttttc ccgcaaaata ttcttcaaac tgttcttgtg tgcgcgttaa aattcgctga
      361 ttcgctgatt cgctttctga aattggctga aaagatacag cagtcaatcc ggattcatct
      421 gcatctaacc ataaaaagcc gataggtgtg tgtattttca ttttgtttgc tcctagtcaa
      481 tagactggaa cttcctcctt tttctctatc caaaactact tgccaactaa taaaaattgt
      541 tcttaaaagt ttgataaacc gaggaa
//
LOCUS       DAXWLF010000340          550 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.349, whole genome shotgun sequence.
ACCESSION   DAXWLF010000340 DAXWLF010000000
VERSION     DAXWLF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.349"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..273
                     /locus_tag="ACGE4U_002841"
     CDS             <1..273
                     /locus_tag="ACGE4U_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFF8394907.1"
                     /translation="SIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            270..>550
                     /locus_tag="ACGE4U_002842"
     CDS             270..>550
                     /locus_tag="ACGE4U_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8394908.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGL"
BASE COUNT          191 a           66 c           95 g          198 t
ORIGIN      
        1 tctatttttt tcgattttgc gctaacaaaa aaaccaatta ttctttttaa ctatgatgaa
       61 aaagaatata gcttagaaag aggtttttat atacagttaa gtgagttacc aattataagc
      121 gtacaagata gttatggcct tgttgatgtt tttaaaaacc aacaagaaaa gcttttgaaa
      181 agttctcaag agttacttga ttatattgga aattatgaaa caggggcaag tacagaaaag
      241 gttcttgagt tgttagagag ggatataaag tgaaaaaaac agtaaaagat atattttata
      301 atgcgatata tcaagtcttt ttgattgttt tgcccttact tactatacct atacttagtc
      361 gtagaatagg ttcaactggt ttagggattt atgggtatgt tttctcaata tcacaatttt
      421 taatgactgt cattgctgtg ggaatgaatc cttttcgtat tcgaaatatc gctaaatcaa
      481 ggaaagataa aaaagcacta tctttacaat tctggaatat ttactttctt caatttttaa
      541 taggcttgag
//
LOCUS       DAXWLF010000341          545 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.350, whole genome shotgun sequence.
ACCESSION   DAXWLF010000341 DAXWLF010000000
VERSION     DAXWLF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.350"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..>545
                     /locus_tag="ACGE4U_002843"
     CDS             <1..>545
                     /locus_tag="ACGE4U_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFF8394909.1"
                     /translation="DYIKRIDSDSFLAAFYLYTAKYWIDDSRKTIKYAKRKKQFYFQT
                     WHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEKYVSAFNIVPSKIL
                     EVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQLEWLGVDNLREFFK
                     KQQQELNLMTKFHPNVHSKLA"
BASE COUNT          209 a           74 c           90 g          172 t
ORIGIN      
        1 agattatatt aaaagaattg attccgattc ttttttagct gccttttatt tgtatacagc
       61 taagtattgg atagacgata gtcggaaaac gattaagtat gcaaaaagga aaaaacaatt
      121 ttattttcaa acatggcacg gtacgccttt aaaaaaaatt gaatttgatg caaaagataa
      181 gttacctaaa cgttacctaa gctatgcgaa aaaagatagt gagtcaatta catatttatt
      241 atctggtaat cgctatagtt ctgagaagta tgtttcagca ttcaatatcg ttccttctaa
      301 aatattagaa gtaggaactc ctagaaacga tgaattagcc tctagtaaag agcaagtgtt
      361 tgacttgaaa aagaaacaaa tcaatgtctt atttgctcca acttttcgaa ataatattga
      421 ggacaacgga attacgcaac tagaatggtt aggtgtggat aacttacgtg aattttttaa
      481 aaagcaacaa caagaattaa atttaatgac taaatttcat cctaatgttc atagtaaatt
      541 agcaa
//
LOCUS       DAXWLF010000342          533 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.351, whole genome shotgun sequence.
ACCESSION   DAXWLF010000342 DAXWLF010000000
VERSION     DAXWLF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.351"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            74..>533
                     /locus_tag="ACGE4U_002844"
     CDS             74..>533
                     /locus_tag="ACGE4U_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFF8394910.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRP"
BASE COUNT          170 a          105 c           99 g          159 t
ORIGIN      
        1 taaaaaaata tagaaaagac accctgaaat cctttagaat taatttggct accaattcat
       61 aggaggattc aagatgtctc atactcagct tatcaaagat accctaaata ttctagacct
      121 aaatatctat ttcgaggaaa attgtttaac taaagaaaaa tacaaagggc aaatttgctt
      181 gatttacaaa ggaactcttc tgtacaagcc agaagagtgt tcccattgtc tttgtgtcgt
      241 cccctctagg atcattcgtt gggggacgac aaccgttcgg ttgttactga atgatgtgtc
      301 tgagtatcgc acttatctgg agctgaagaa acaacgattt aaatgtaagt cttgccaaag
      361 aacattcgta gcggatacct ctgttgctga aaaacactgt ttcattagtc aaaaagttcg
      421 ttggtctgtc attgctcgct taaaagagaa tacttccatg acggaaatcg ctcgtcagaa
      481 aaatatttcc acttcttcgg tctatcgtgt catgaagcga ttctaccgcc cat
//
LOCUS       DAXWLF010000343          531 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.352, whole genome shotgun sequence.
ACCESSION   DAXWLF010000343 DAXWLF010000000
VERSION     DAXWLF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.352"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..>531)
                     /locus_tag="ACGE4U_002845"
     CDS             complement(<1..>531)
                     /locus_tag="ACGE4U_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394911.1"
                     /translation="LAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSF
                     GKPFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTY
                     VNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNK
                     KTSIFLVILMIMGFCS"
BASE COUNT          217 a           84 c           68 g          162 t
ORIGIN      
        1 ttgaacaaaa tcccattatc atcaaaataa ctaaaaaaat agaagttttc ttattgtcaa
       61 aacttaaata aattccaaca attaaacata gtaataatgc caagactact aaagtagcta
      121 tccaagtacc tggataaaaa agtgacagtt tcgttcctaa ctctgtaaag agattattca
      181 ggttactact actccaaatc attgcccttt tatttccatt gacgtaagta aagctttgac
      241 ccattgtatt accaaaataa ataatcaggt ttaagaaaaa cggtaatgca gtcaatatac
      301 gcacataata gttttgacat ttcctatatg ttagaaggaa aattaagaaa aatagcaata
      361 aaaataagat attcatgtct aaaaaaaacg gctttccaaa tgatgaaata cctaaatcta
      421 acttattaag aaagttgaaa tttttatatt caggaaacca tttattaatt tcatgtgcag
      481 cacgtaatgc attccctgga gttgtcaaag agaaaattaa ttctgctaag c
//
LOCUS       DAXWLF010000344          521 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.353, whole genome shotgun sequence.
ACCESSION   DAXWLF010000344 DAXWLF010000000
VERSION     DAXWLF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.353"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..164)
                     /locus_tag="ACGE4U_002846"
     CDS             complement(<1..164)
                     /locus_tag="ACGE4U_002846"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8394912.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINAL"
BASE COUNT          176 a           99 c           67 g          179 t
ORIGIN      
        1 ataagcgcat taatatgcgg ctgaataatt tcttcaaata acgcttcctt agaagtaaaa
       61 tgtttataaa aagcacccgt agttacgcct gcggtcaggc acagttgcct tatcttagtt
      121 tgctgaaatc cttctttttc aaaatggctt agtccacttt ctattaactt ttggtgagtt
      181 ataatataat ctctggtttc tatgggtact tcctcctaat aagatgaatt aactttatta
      241 tcttacacag gacttgaagt ttcaatcgtt aacgtttatt actgaactca ttccttagag
      301 ctaaactaat gagaaagagc agaataactg atacaaaaag caatcctaca agaattaata
      361 gactcatcta aatcattcac cttttctaaa ataatccgag aaagatttaa aaaaattata
      421 tccagctatt aggataagtc ctatcataat cactccaata gccattttct tatcctccct
      481 aaaaataaag tcttttactt aaaacctttt ttgtaatcgt c
//
LOCUS       DAXWLF010000345          508 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.354, whole genome shotgun sequence.
ACCESSION   DAXWLF010000345 DAXWLF010000000
VERSION     DAXWLF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.354"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..93
                     /locus_tag="ACGE4U_002847"
     CDS             <1..93
                     /locus_tag="ACGE4U_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFF8394913.1"
                     /translation="VVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            165..>508
                     /locus_tag="ACGE4U_002848"
     CDS             165..>508
                     /locus_tag="ACGE4U_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394914.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          225 a           66 c           75 g          142 t
ORIGIN      
        1 gttgttttag aaaattataa tggaacagta acatttacaa aattagctaa agctgaacaa
       61 tatagacgat ctttagaatt gtcaaaagat taaatatata aaaagccttc gggctttttc
      121 ttttaacaat taataggaac gtatattcga aaggaataga aataatgtgg ttagaaaaaa
      181 taaatgacaa aaaatttaag tacaccgaac gttatacgga tcccttaact gaaaaaaaga
      241 gaaaagtgtc cgttacttta tctagcaatt cacgccaagc ttggaatcaa gctaacttac
      301 ttttgaatga aaaaatcgca gaaaaaataa aacgaaatga agaattacca ctaacttttg
      361 gagaattaaa aactaaatgg gatgaaaaat acaagccaac tgtaaaagaa agttcctatc
      421 gaacaactca ggtttatcta tcattaattt caaaatatat aaaagatgat gtacttgtaa
      481 aaaacgttaa tagtaatctt atacaaga
//
LOCUS       DAXWLF010000346          499 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.355, whole genome shotgun sequence.
ACCESSION   DAXWLF010000346 DAXWLF010000000
VERSION     DAXWLF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.355"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..>499)
                     /locus_tag="ACGE4U_002849"
     CDS             complement(<1..>499)
                     /locus_tag="ACGE4U_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFF8394915.1"
                     /translation="KLDDSPSYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRT
                     RFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTR
                     ILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYK
                     LTQYFL"
BASE COUNT          144 a          101 c          105 g          149 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaattt
//
LOCUS       DAXWLF010000347          495 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.356, whole genome shotgun sequence.
ACCESSION   DAXWLF010000347 DAXWLF010000000
VERSION     DAXWLF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.356"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            29..226
                     /locus_tag="ACGE4U_002850"
     CDS             29..226
                     /locus_tag="ACGE4U_002850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394916.1"
                     /translation="MGQYFLTGVWYVLKLVIILGIAYICFRILLSGEGMPTPKDFIIS
                     VLNFVADQIQVIIHWAQQRFG"
     gene            268..396
                     /locus_tag="ACGE4U_002851"
     CDS             268..396
                     /locus_tag="ACGE4U_002851"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394917.1"
                     /translation="MIENFEYKLSRLPKKKGATQKELANKLGVNVQSVSNVEEDKS"
BASE COUNT          175 a           51 c          105 g          164 t
ORIGIN      
        1 actttaaaga aaggaatatg ttggaaaaat gggacagtat tttcttaccg gtgtttggta
       61 tgtattgaaa ttagtgatta ttttaggtat tgcctatatt tgttttagaa ttttattatc
      121 gggagaagga atgccaacgc caaaagattt tatcataagc gtgttgaatt ttgtggcgga
      181 tcaaatacaa gtgataattc attgggcgca gcaacgtttt ggataaaaaa atatatttaa
      241 aaaatttaat gtttagaagt gggctatgtg atagaaaatt tcgagtataa gttatctaga
      301 ttgccaaaga aaaagggagc tacacaaaaa gagctggcta ataaattagg tgtcaatgta
      361 caaagcgttt ctaatgttga ggaagataag agctaactac atttagtaat ttagaaaaaa
      421 acagtttctt tctttgatgc ttcatcgatt gaattatttg gtacgaatag ggatcgaata
      481 attgcagatg ttcca
//
LOCUS       DAXWLF010000348          486 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.357, whole genome shotgun sequence.
ACCESSION   DAXWLF010000348 DAXWLF010000000
VERSION     DAXWLF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.357"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(210..446)
                     /locus_tag="ACGE4U_002852"
     CDS             complement(210..446)
                     /locus_tag="ACGE4U_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394918.1"
                     /translation="MDKGVADIAKIKQVLKQESIKSLVEGTGLSKSTISSLKSGTRKV
                     EKLNLFAAIKLTEYSDQVFKPIIEIWGKELKKQL"
BASE COUNT          164 a           73 c           57 g          192 t
ORIGIN      
        1 gaaactacag aatttgaaga agatcttctg aaatatctga aaatattggt aaaaaaaaga
       61 ctcattgaga ttgaataaaa ttataacttt ataattggta gaactgagaa atattatctt
      121 tcagttctac ccattgtaga gctaaacaag cattcatctg aaaaatatct agttcttcta
      181 tttttaaatc tattagtttt attcgataac tatagttgtt tttttagttc ttttccccag
      241 atttcaataa taggtttaaa aacttgatct gaatattcgg ttagtttaat agctgcaaaa
      301 aggtttaatt tttctacttt tcgagtacct gattttaaac ttgaaatagt actcttactt
      361 aaaccagttc cttctactaa gctttttata gattcttgtt tcaacacttg cttaattttt
      421 gcaatatcag cgactccttt atccaaaaaa agactccttt tttatctcaa atttataact
      481 actgta
//
LOCUS       DAXWLF010000349          463 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.358, whole genome shotgun sequence.
ACCESSION   DAXWLF010000349 DAXWLF010000000
VERSION     DAXWLF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.358"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..>463
                     /locus_tag="ACGE4U_002853"
     CDS             <1..>463
                     /locus_tag="ACGE4U_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFF8394919.1"
                     /translation="RKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSC
                     GEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCP
                     FDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYE"
BASE COUNT          148 a           78 c           92 g          145 t
ORIGIN      
        1 tcgtaaattc ttaagagaca acccattaga agcgatcgta acagacttga cttatgtacg
       61 agtcgggaaa aaatggaatt atgtctgttt cattttggat ctgttcaatc gagaaattct
      121 cggctattct tgtggagaac ataaagatgc cgttctagta aaaaaagcat ttagccgtat
      181 caaacaacct ctgacagagg ttgagatttt tcatactgat cgtggaaaag agtttgataa
      241 ccaagctatt gatgaattat taacaacttt tgacatcaat cgatcattga gtcataaagg
      301 ctgtcctttt gataatgccg tagctgaatc aacttataag tcgttgaaag tagaatttgt
      361 ctatcaatac acatttgaaa ccttacaaca attggatttg gagttatttg actatgtcaa
      421 ttggtggaac caccttcggt tgcacggtac acttggctac gag
//
LOCUS       DAXWLF010000350          461 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.359, whole genome shotgun sequence.
ACCESSION   DAXWLF010000350 DAXWLF010000000
VERSION     DAXWLF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.359"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..431
                     /locus_tag="ACGE4U_002854"
     CDS             <1..431
                     /locus_tag="ACGE4U_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFF8394920.1"
                     /translation="KWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKRLVKQFDEPK
                     VVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTA
                     STTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          154 a           87 c           94 g          126 t
ORIGIN      
        1 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
       61 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      121 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      181 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      241 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      301 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      361 gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa
      421 tcccagctta aatcatagat accgtaaggg attttattct t
//
LOCUS       DAXWLF010000351          441 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.360, whole genome shotgun sequence.
ACCESSION   DAXWLF010000351 DAXWLF010000000
VERSION     DAXWLF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.360"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..388
                     /locus_tag="ACGE4U_002855"
     CDS             <1..388
                     /locus_tag="ACGE4U_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HFF8394921.1"
                     /translation="DVNYSKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGY
                     YKGNNFEDTQITMLENKSVKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTN
                     NAYYYELKKDGRVYGYINSEAAVEMN"
BASE COUNT          171 a           58 c           87 g          125 t
ORIGIN      
        1 agatgtaaat tattcaaaag gaaaaactga aagaggccga gtattttaca ttagtatttc
       61 atataatacc gcagataacc aaccgattta ttcagtatat acagatgaaa catgtacgga
      121 atggcgaggg tattataagg gaaacaactt tgaagacact caaattacta tgcttgagaa
      181 taaaagcgtg aaagtaacaa aaagtgggta tacagtatgg ggagacttga atttttggac
      241 taagtcagga atatcgaaca caggagatat atacaccact gaccgaaaat tttataattt
      301 taccaacaat gcttactatt atgaattgaa aaaagatggg agggtctatg gctatataaa
      361 tagtgaagca gctgtagaga tgaactaaaa tattgctgtc ccttaatctt atggggcagc
      421 aatattttta tattattaat c
//
LOCUS       DAXWLF010000352          441 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.361, whole genome shotgun sequence.
ACCESSION   DAXWLF010000352 DAXWLF010000000
VERSION     DAXWLF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.361"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            167..364
                     /locus_tag="ACGE4U_002856"
     CDS             167..364
                     /locus_tag="ACGE4U_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFF8394922.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            366..>441
                     /locus_tag="ACGE4U_002857"
     CDS             366..>441
                     /locus_tag="ACGE4U_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFF8394923.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYS"
BASE COUNT          168 a           44 c           80 g          149 t
ORIGIN      
        1 tgcattctca aaaaatatta aagaatatac atagtttata tacttaaaag tgattaaatt
       61 cttattcagg aattattttg ctacgtttca agaaggaatt gttttttgaa atttaatgaa
      121 tgttagaata atagtctaaa aacattaatt taggggtgat tagattatga aacatttaaa
      181 aattttgtct attaaagaga cacaacttat ctatgggggt accactcata gtggaaaata
      241 ttatggaaat ggagtgtatt gcactaaaaa taaatgtacg gtcgattggg ccaaggcaac
      301 tacttgtatt gcaggaatgt ctataggtgg ttttttaggt ggagcaattc cagggaagtg
      361 ctaaaatgaa aaaaaatgct aagcaaattg ttcatgaatt atataatgat atatctataa
      421 gtaaagatcc taaatattct g
//
LOCUS       DAXWLF010000353          434 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.362, whole genome shotgun sequence.
ACCESSION   DAXWLF010000353 DAXWLF010000000
VERSION     DAXWLF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.362"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..>434)
                     /gene="scm"
                     /locus_tag="ACGE4U_002858"
     CDS             complement(<1..>434)
                     /gene="scm"
                     /locus_tag="ACGE4U_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFF8394924.1"
                     /translation="VNIDAEVLTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYN
                     DRSNHETEDFSDANISWNFGNEMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNV
                     ILDAATNRWDPNEIFISDNGTDTTDTSDSTDTSTTSDSSDSS"
BASE COUNT          118 a           77 c           86 g          153 t
ORIGIN      
        1 tgatgaatct gagctatcac ttgtggtaga tgtatctgta ctgtcgcttg tgtctgtcgt
       61 atctgtacca ttgtcgctga taaatatttc gttaggatcc caacgattgg tagctgcatc
      121 taaaatcaca tttgttccta taatcgttcc gtcttggttt tgagtaatgc ggatagtagc
      181 attaggcgca aggattgttc cttgaaaagg agcactgatt gtcaaatcag tcatctcatt
      241 tccaaaattc caagagatat tcgcatccga aaaatcttct gtttcatgat ttgaacgatc
      301 attgtatttg attggtgact gtacgttcaa tgcagaagaa ctgttgatga cattaaatac
      361 aactacttga tcatttggat taatgatttg aagtggcgta ttcatcgtta acacttctgc
      421 atcgatattt acca
//
LOCUS       DAXWLF010000354          432 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.363, whole genome shotgun sequence.
ACCESSION   DAXWLF010000354 DAXWLF010000000
VERSION     DAXWLF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.363"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            115..>432
                     /locus_tag="ACGE4U_002859"
     CDS             115..>432
                     /locus_tag="ACGE4U_002859"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8394925.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNL"
BASE COUNT          163 a           53 c           75 g          141 t
ORIGIN      
        1 tcctattctt aaaatgatgt ttgtacttac ttaaattagg tataattagt atatgttttg
       61 tgagaaattt agacttacaa taagcagtga atttataatc aacgtatagg agacatggat
      121 gaaatgaaac taattggtat agatttagac ggtactcttt tgaattctga ccagagcatt
      181 agtcaaaaaa atgcaagaac aatgaaacat ttaccggaag actgttttcc attcatttgt
      241 tctggaagag aagtagaaga cattaataat attctcaaaa aatctggttt aaggatacca
      301 gcagtagggt taaatggttc tgttggattt gatggagaaa aaaagatatt tgagtcttcc
      361 ttcgataaaa atgatgtaaa aaaaattgat tctgtcctat cgaaatttcc aaccaaaata
      421 tatacaaatc tc
//
LOCUS       DAXWLF010000355          427 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.364, whole genome shotgun sequence.
ACCESSION   DAXWLF010000355 DAXWLF010000000
VERSION     DAXWLF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.364"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..>427)
                     /locus_tag="ACGE4U_002860"
     CDS             complement(<1..>427)
                     /locus_tag="ACGE4U_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8394926.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDEL"
BASE COUNT          125 a           76 c           87 g          139 t
ORIGIN      
        1 agttcatcca atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc
       61 gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga
      121 aaatgttgct ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc
      181 tgtaaggcca tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag
      241 aaatcagcga ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta
      301 aattgtttca cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc
      361 tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca
      421 atcacaa
//
LOCUS       DAXWLF010000356          415 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.365, whole genome shotgun sequence.
ACCESSION   DAXWLF010000356 DAXWLF010000000
VERSION     DAXWLF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.365"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..339)
                     /locus_tag="ACGE4U_002861"
     CDS             complement(<1..339)
                     /locus_tag="ACGE4U_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8394927.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAXWLF010000357          409 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.366, whole genome shotgun sequence.
ACCESSION   DAXWLF010000357 DAXWLF010000000
VERSION     DAXWLF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.366"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
BASE COUNT          134 a           32 c           51 g          192 t
ORIGIN      
        1 taattttaat ggtagtacat aattattata tatatctgac attggtagta cttattgtat
       61 tttttagtag tatataatct attgttttct agcctaaagc cttgtcttat aagaataata
      121 taatatatac tactgaaata aggtagtata tattactagt aatggtagta tgttttatat
      181 gcataaggta gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta
      241 attttaaaac taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt
      301 tatttaaagt agttcttaat gactttaaag gtagtacata attcatttta tttctgattt
      361 taggtagtat ttaattaaaa aacagtttta atgcgtttgc attttttat
//
LOCUS       DAXWLF010000358          407 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.367, whole genome shotgun sequence.
ACCESSION   DAXWLF010000358 DAXWLF010000000
VERSION     DAXWLF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.367"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            176..>407
                     /locus_tag="ACGE4U_002862"
     CDS             176..>407
                     /locus_tag="ACGE4U_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394928.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEK"
BASE COUNT          176 a           56 c           57 g          118 t
ORIGIN      
        1 ttatttgaaa aaacatttgc aataaatcag aaaatagtgg tcgtttattt gtattcgcta
       61 ttctaataat aaataaccac tatttgattt atatatattt cagtatcctt cttgttttca
      121 tataaccaaa ggcgctatga tttccatatg aaaaaaataa agtcaggtga aaaaaatgaa
      181 acttgaggat aaaaaagtca ttttgaagaa aatcaaacga ctaccagacc aaatagatgg
      241 ctatacgatc tgttctttca aaaaagacag gagtataaaa gcttatacgc aaaacacaga
      301 aaaaggaata atctatttcc tacatgaaga aggttacgat acccaaacct ttatgtttac
      361 tgaaaaaaaa gaatttcata aacagatgaa aaaactgatt gaaaaag
//
LOCUS       DAXWLF010000359          405 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.368, whole genome shotgun sequence.
ACCESSION   DAXWLF010000359 DAXWLF010000000
VERSION     DAXWLF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.368"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
BASE COUNT          166 a           48 c           57 g          134 t
ORIGIN      
        1 gcgcatactt ttatgagtga ttgaatgtga ttttaataca tataccaatt aaaagaaaac
       61 tttttagatt tttacttaca aatttataaa aaaacaagat aattgatact ttgattttgc
      121 gaacatttta tctttccgcc taattcagct ttaatatcat gattatatca gtaaaaaatc
      181 gtttttttag ctaatttaag catcaaataa ttattcttaa tcaatgaaaa aataactaat
      241 aaattaattt tgggaatagg taaaaacttg ttcggtttct ggataaaaat aacatgaaaa
      301 aggaaatata aaataacatg gagcgtggga ggaataaaga aattttacac gcaaggtgcg
      361 taggaatgta aagaaactta acacgaaaca aaagtcctat tccct
//
LOCUS       DAXWLF010000360          395 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.369, whole genome shotgun sequence.
ACCESSION   DAXWLF010000360 DAXWLF010000000
VERSION     DAXWLF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.369"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..382
                     /locus_tag="ACGE4U_002863"
     CDS             <1..382
                     /locus_tag="ACGE4U_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8394929.1"
                     /translation="KEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIEN
                     QDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYN
                     QKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          152 a           71 c           59 g          113 t
ORIGIN      
        1 aaaagaaatc tgtgatgact ttaaggctgt ttatcaagct aactcaaaag aagaagcgaa
       61 taccttctta tccggcatga ttgagaaatg gaagaaaaac tatcctaaag tgacgcagtc
      121 actcatagaa aaccaagact tattaacttt ttatgatttt ccacctagca ttcgtagaac
      181 catttactca accaatctaa tcgagtcttt caataagcaa attaaaagat acagccgtag
      241 aaaagagcag tttcaaaatg aagaatcact agaacgcttt ctagtcagca tttttgatac
      301 atacaatcaa aaatttctaa acagaagcca taaaggtttt caacaggtaa ccgatacatt
      361 agtttcaatg tttactgagt aactaattat tttgc
//
LOCUS       DAXWLF010000361          393 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.370, whole genome shotgun sequence.
ACCESSION   DAXWLF010000361 DAXWLF010000000
VERSION     DAXWLF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.370"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            4..198
                     /locus_tag="ACGE4U_002864"
     CDS             4..198
                     /locus_tag="ACGE4U_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394930.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          164 a           53 c           73 g          103 t
ORIGIN      
        1 caaatgtatt atgtagaagt aaaaacaaaa ggcgtcaaaa acaaacaata tgtgaaaggt
       61 atcagtaacg aatatccttt acttggttca tggaaagaag ctgctccttt ttccaaacca
      121 tgtgccataa aaataaagaa cgagctagaa aaagaactga cttgtggtaa agctgttgta
      181 gatatcatcg aaaagtaaat ccaatgtata gaaaggaaga tcacacatga aaagcgacgt
      241 acaaattgct aagattatag aattagaaga agaagacgca gtgttgacaa ttgaaaaaaa
      301 ataaatgttt gtataccata tgattatttc aatatgtatc ctggaattgg agatctcgta
      361 gagctatatc aagatgaatc aaatatactt gtc
//
LOCUS       DAXWLF010000362          390 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.371, whole genome shotgun sequence.
ACCESSION   DAXWLF010000362 DAXWLF010000000
VERSION     DAXWLF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.371"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..331
                     /locus_tag="ACGE4U_002865"
     CDS             <1..331
                     /locus_tag="ACGE4U_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8394931.1"
                     /translation="ESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILA
                     DEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVN
                     KMNLEVE"
BASE COUNT          164 a           37 c           74 g          115 t
ORIGIN      
        1 tgaaagtatc ttgtttgaac taggtttaga taaaaaacaa ataaaaagaa atgttatgaa
       61 attatctggt ggacagcaac aaagagtagc tattgctaga gcattggtat cagatgctcc
      121 aataatacta gctgatgagc ctactggtaa tctagacagt gttactgctg gagaaataat
      181 taatatatta aagacattag ctaaagatag aaataaatgt gtaatagttg taacacatag
      241 taaggaagta gcagattctg cggatatcat tttagaacta agtggtaaaa agttgaaaaa
      301 agtaaataaa atgaatttgg aggttgaata atgataaaaa atgcatttgc ttatgtaact
      361 agaaaaagtt taaaatcatt aattatcata
//
LOCUS       DAXWLF010000363          387 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.372, whole genome shotgun sequence.
ACCESSION   DAXWLF010000363 DAXWLF010000000
VERSION     DAXWLF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.372"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            9..>387
                     /locus_tag="ACGE4U_002866"
     CDS             9..>387
                     /locus_tag="ACGE4U_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFF8394932.1"
                     /translation="MWFYYSQALYTKTKNMKNRIRLNTLLNAKSGLCNEDCGYCAQSK
                     KSKATIQKYSLLPQDEIIRKALIAKKNNSSVFCIALSGTRPTQKEVLVLGETIKEIKR
                     IMDIEICLSIGLITDNQIRYLKKC"
BASE COUNT          159 a           51 c           64 g          113 t
ORIGIN      
        1 aagaaaatat ttggttttac tattcgcaag ccctatatac taaaactaaa aatatgaaaa
       61 atagaataag gttaaataca cttctaaatg ctaaaagtgg attatgtaat gaagattgtg
      121 gttactgcgc ccaatctaaa aaaagtaagg cgaccattca aaaatacagt cttttacccc
      181 aagatgagat tatacgtaaa gcattgatag caaaaaagaa taattcaagt gttttttgta
      241 ttgcgttaag tggaactaga cctactcaaa aagaagtatt agttctcggc gaaacaatta
      301 aagaaattaa gcgcattatg gatatcgaga tttgtttatc tattggatta atcacagata
      361 atcagataag atacttaaaa aaatgcg
//
LOCUS       DAXWLF010000364          386 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.373, whole genome shotgun sequence.
ACCESSION   DAXWLF010000364 DAXWLF010000000
VERSION     DAXWLF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.373"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..>386
                     /locus_tag="ACGE4U_002867"
     CDS             <1..>386
                     /locus_tag="ACGE4U_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFF8394933.1"
                     /translation="GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQW
                     IQFWKKAMKLDYDPNVKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLK
                     RLSDLEEGLHRKRLISYGGLLKEIHK"
BASE COUNT          156 a           47 c           74 g          109 t
ORIGIN      
        1 ttggttttat gcgtgcaacg gaagtgacaa taaataataa agataattct tataatcagc
       61 acatgcatgt attggtatgt gtggaaccaa cttattttaa gaatacagaa aactacgtga
      121 atcaaaaaca atggattcaa ttttggaaaa aggcaatgaa attagactat gatccaaatg
      181 taaaagttca aatgattcga ccgaaaaata aatataaatc ggatatacaa tcggcaattg
      241 acgaaactgc aaaatatcct gtaaaggata cggattttat gaccgatgat gaagaaaaga
      301 atttgaaacg tttgtctgat ttggaggaag gtttacaccg taaaaggtta atctcctatg
      361 gtggtttgtt aaaagaaata cataaa
//
LOCUS       DAXWLF010000365          374 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.374, whole genome shotgun sequence.
ACCESSION   DAXWLF010000365 DAXWLF010000000
VERSION     DAXWLF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.374"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
BASE COUNT          147 a           54 c           69 g          104 t
ORIGIN      
        1 ctgttattca aatcatttta tcaaaactac atagtaaatg ttaaaaagca acatgacata
       61 atatatagaa ggctttacag tatatatggg agcttactaa aaaagaatgg agtaagagtt
      121 aacacaatgg gccattagct gaagttttaa gcacttcaag gtatgaaaca gaatggcctg
      181 taatactaca tgattattta gactgctcaa actgctatca gtctggtgga atgaataaag
      241 tattatacta aaagcagatc caactacaac gtaacaggaa accagttgta tcaggaaaag
      301 tgatagtaca tggagaagaa aaggccccct ttgtatgagt cagtacatat cttatttata
      361 gataaaacgg tatt
//
LOCUS       DAXWLF010000366          353 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.375, whole genome shotgun sequence.
ACCESSION   DAXWLF010000366 DAXWLF010000000
VERSION     DAXWLF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.375"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
BASE COUNT          129 a           39 c           52 g          133 t
ORIGIN      
        1 tttaatgata aagtgatatt tactcaaaat ttaaaaacaa ataatgaaaa tgaaaagctt
       61 tatgaaccaa caaatattga tacccaagat gaaaaaacta ataatacaac aaaagtttat
      121 gacggttggt attatttagg ctctttgtca aataggactg atagagtgat ataagaacat
      181 cggctctata attatgagta ttatgctgga ttagacgtcc atcaaaataa ttttttgttt
      241 tgaaaggttc ctttattcag ataaatagga tgttggagta tatctatgtt gcttatgtaa
      301 tacaatttat ttttattgta tatttgttcc cttttcgtac ttttaaactt tta
//
LOCUS       DAXWLF010000367          345 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.376, whole genome shotgun sequence.
ACCESSION   DAXWLF010000367 DAXWLF010000000
VERSION     DAXWLF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.376"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            21..>345
                     /locus_tag="ACGE4U_002868"
     CDS             21..>345
                     /locus_tag="ACGE4U_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010709177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFF8394934.1"
                     /translation="MSLMLALLIITARLVIPLPLFDYLSLQIITVYLIYPTLGMKNGL
                     IVTCSYLILGIIGLPIFASGGGYAYIFKPTFGFLLAFVTLPIIQSFISNKFNNAIYSS
                     FKKNAI"
BASE COUNT           99 a           57 c           50 g          139 t
ORIGIN      
        1 agagtcaaat gattgcgcga atgtcattaa tgttggcgct gttaattatt acagctagac
       61 ttgttattcc tttaccttta tttgattatt tatctttaca gattattact gtttatctca
      121 tttatcctac attaggtatg aaaaacggtc tgatcgtaac ctgtagctac ttaattctag
      181 gaataatagg tcttccgata tttgcaagcg gtggtggata tgcatatatt ttcaaaccaa
      241 cttttggttt cttgctagcc tttgtgactt tacctattat acaatcgttc atctcaaata
      301 aatttaataa tgctatctac tcttctttta aaaaaaatgc tatta
//
LOCUS       DAXWLF010000368          344 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.377, whole genome shotgun sequence.
ACCESSION   DAXWLF010000368 DAXWLF010000000
VERSION     DAXWLF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.377"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACGE4U_002869"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACGE4U_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFF8394935.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          107 a           51 c           56 g          130 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgg
//
LOCUS       DAXWLF010000369          338 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.378, whole genome shotgun sequence.
ACCESSION   DAXWLF010000369 DAXWLF010000000
VERSION     DAXWLF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.378"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(2..>338)
                     /locus_tag="ACGE4U_002870"
     CDS             complement(2..>338)
                     /locus_tag="ACGE4U_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394936.1"
                     /translation="SVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKS
                     ISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNN
                     NFYFYRNKL"
BASE COUNT          142 a           43 c           51 g          102 t
ORIGIN      
        1 attacaattt attgcgataa aaataaaagt tgttattttt attgggaaaa tacccgatgt
       61 agaaaataat gccttccaat atacctataa aataagtcca gtattccaaa ggattatata
      121 aataagtgac gtatctatct aaaggtgtaa taaaaatgat aagtgataat aaaaaaatac
      181 catttataac taaacataaa ctaatggatt tgggcggttt caggctcatc agaaaaatat
      241 agattctggt gaacaacaac cataaaaaag tgagaatcat tgcatgagag cttctttcca
      301 taaattttcc tttaagagta gtgcttaaaa gtacagaa
//
LOCUS       DAXWLF010000370          309 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.379, whole genome shotgun sequence.
ACCESSION   DAXWLF010000370 DAXWLF010000000
VERSION     DAXWLF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.379"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            1..309
                     /locus_tag="ACGE4U_002871"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACGE4U_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAXWLF010000371          299 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.380, whole genome shotgun sequence.
ACCESSION   DAXWLF010000371 DAXWLF010000000
VERSION     DAXWLF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.380"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
BASE COUNT           94 a           57 c           71 g           77 t
ORIGIN      
        1 gagctcggat gtgctaacgc tagcgaggca gtctaacttt aactgttagc tcctgccgaa
       61 gatcgtcaca tccgaggagg ctcattgtca aggacaatcg gaatagcata cggaaagaag
      121 ttctgcacct tataaaattt gtcaaaaaaa ctgttgccat tccaatgaga aaaagcatgg
      181 aatgcgctgc atcaggtatc gaggcttaga aaaaaatcag gtgcatacga tgcttgtttt
      241 tgccgaaatg agtctgaaaa aattggtcac ttagcagtgg gagcatatgt cttcatatt
//
LOCUS       DAXWLF010000372          288 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.381, whole genome shotgun sequence.
ACCESSION   DAXWLF010000372 DAXWLF010000000
VERSION     DAXWLF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.381"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..>288
                     /locus_tag="ACGE4U_002872"
     rRNA            <1..>288
                     /locus_tag="ACGE4U_002872"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           67 a           70 c           80 g           71 t
ORIGIN      
        1 gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca tcctttgacc
       61 actctagaga tagagcttcc ccttcggggg caaagtgaca ggtggtgcat ggttgtcgtc
      121 agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt attgttagtt
      181 gccatcattc agttgggcac tctagcaaga ctgccggtga caaaccggag gaaggtgggg
      241 atgacgtcaa atcatcatgc cccttatgac ctgggctaca cacgtgct
//
LOCUS       DAXWLF010000373          283 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.382, whole genome shotgun sequence.
ACCESSION   DAXWLF010000373 DAXWLF010000000
VERSION     DAXWLF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.382"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
BASE COUNT          117 a           31 c           50 g           85 t
ORIGIN      
        1 tctaaatgat tcctttaagg tgtcactaaa aattataaaa aatagtgttg acgcactttt
       61 gatgtcatgt tataataacg aagtcgtttg gatgagatag ctaataaagc ttgattttat
      121 cgaaaaaaac ttcgaaaaag ttattgacaa ataacaataa agatggtatg ataatcaagt
      181 cgctgctaat gagtggcaaa gacgtttaga aaaaataaac aaagaagttg ttgacattct
      241 cttctgaatc tgataagatt aaagagttgt taaaacaaaa aga
//
LOCUS       DAXWLF010000374          266 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.383, whole genome shotgun sequence.
ACCESSION   DAXWLF010000374 DAXWLF010000000
VERSION     DAXWLF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.383"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
BASE COUNT          100 a           32 c           38 g           96 t
ORIGIN      
        1 ttaaataccg aaaaaattat gtttagataa taccatagtt aagtttttcc ttgtgatgtt
       61 tttcgtaaaa agatatttta gcagcattat cgttacagtg catagttacc atcggcgtta
      121 tgactatata aagtatactt tcgttgatcc agaaaggtat atagattaaa ataagttacc
      181 taaaaaggac aaatacaaat tttattatct tattcacagt aagaagataa ttttaagatt
      241 gcgaaatacg atactatttg taatct
//
LOCUS       DAXWLF010000375          265 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.384, whole genome shotgun sequence.
ACCESSION   DAXWLF010000375 DAXWLF010000000
VERSION     DAXWLF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.384"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..>265)
                     /locus_tag="ACGE4U_002873"
     CDS             complement(<1..>265)
                     /locus_tag="ACGE4U_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8394937.1"
                     /translation="NVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFY
                     PFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVS"
BASE COUNT           97 a           45 c           36 g           87 t
ORIGIN      
        1 aactaactat acttgttaga acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa
       61 atatcaataa aggaatactt acccaatata tttttctatt tatttcttct cctcttaaga
      121 gttgaaaaaa cggataaaaa gttagtaaac taaatgctac tggccactga tagttcaagg
      181 tggttgctct ccaaccggtt tcccccatta ctgtcaatgg tattaaacag tatatagagc
      241 aaaccaataa taaattttta acatt
//
LOCUS       DAXWLF010000376          257 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.385, whole genome shotgun sequence.
ACCESSION   DAXWLF010000376 DAXWLF010000000
VERSION     DAXWLF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.385"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..>257
                     /locus_tag="ACGE4U_002874"
     CDS             <1..>257
                     /locus_tag="ACGE4U_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFF8394938.1"
                     /translation="STIDRLKKEIRFYNRVIGIGNIIKIVLSASIPILIDQASEHRSL
                     LLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK"
BASE COUNT          102 a           32 c           39 g           84 t
ORIGIN      
        1 ttctacaatc gatagattaa aaaaagaaat tcgattttat aatagagtaa ttggtatcgg
       61 aaatattata aaaatagtct tatcagctag tatcccaata ttaattgatc aggcttccga
      121 acacaggtct ttactattga ttgtttcaat tgcttcagca ataattacaa ttatacaaag
      181 tgggatgtct gcatttaatt atcaagataa agtacagact tcgacagagt tattaatgaa
      241 aattgaaaaa gaaaagt
//
LOCUS       DAXWLF010000377          252 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.386, whole genome shotgun sequence.
ACCESSION   DAXWLF010000377 DAXWLF010000000
VERSION     DAXWLF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.386"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            27..>252
                     /locus_tag="ACGE4U_002875"
     CDS             27..>252
                     /locus_tag="ACGE4U_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8394939.1"
                     /translation="MYKAANDLFNRLKLEKKELILKYILDEFGRVGYEATSVRKIASK
                     ADLSVGSLYQYFNNKEGMLTVAIEHVFVILD"
BASE COUNT           92 a           26 c           49 g           85 t
ORIGIN      
        1 aaaatacctt ttttgaggag atcattatgt ataaagcagc gaacgattta tttaatcgat
       61 taaaattaga aaaaaaggaa ctcattttaa agtatatttt agatgagttt gggagagtag
      121 gttatgaagc cacttctgtt cgtaaaattg ccagtaaagc agatttaagt gtaggatcat
      181 tatatcagta tttcaacaat aaagaaggta tgttaacagt cgcgatagaa cacgtttttg
      241 ttattttaga tg
//
LOCUS       DAXWLF010000378          247 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.denovo.387, whole genome shotgun sequence.
ACCESSION   DAXWLF010000378 DAXWLF010000000
VERSION     DAXWLF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.denovo.387"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..>247
                     /locus_tag="ACGE4U_002876"
     CDS             <1..>247
                     /locus_tag="ACGE4U_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8394940.1"
                     /translation="PIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRI
                     GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTF"
BASE COUNT           80 a           51 c           61 g           55 t
ORIGIN      
        1 ccgatcgaaa atgagtccga cttagaagag atcgttcagg aagagtttat tcgaaaccga
       61 aaggcttacg gtacccgaaa attgaagaag tgtttagcaa agcgtgggct tcaactcagt
      121 cggcgccgaa tcggtcgaat catgaaacgc cgcggattga catctaccta tacgatcgct
      181 cattttaaag ggcaacgaac agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg
      241 accttta
//
LOCUS       DAXWLF010000379         7378 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.guided.130, whole genome shotgun sequence.
ACCESSION   DAXWLF010000379 DAXWLF010000000
VERSION     DAXWLF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.guided.130"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..362
                     /locus_tag="ACGE4U_002877"
     CDS             <1..362
                     /locus_tag="ACGE4U_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFF8394941.1"
                     /translation="YTDFGKHISIGKNVFINRQVMFVDLGGICLEDSVLIGPRVNLIT
                     VNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAANATVTKDVPDNT
                     IVAGIPAKIVKPVERII"
     gene            429..1166
                     /locus_tag="ACGE4U_002878"
     CDS             429..1166
                     /locus_tag="ACGE4U_002878"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8394942.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            1397..2665
                     /locus_tag="ACGE4U_002879"
     CDS             1397..2665
                     /locus_tag="ACGE4U_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFF8394943.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            3152..3616
                     /locus_tag="ACGE4U_002880"
     CDS             3152..3616
                     /locus_tag="ACGE4U_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8394944.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            3600..4229
                     /locus_tag="ACGE4U_002881"
     CDS             3600..4229
                     /locus_tag="ACGE4U_002881"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8394945.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            4583..6061
                     /gene="msr(C)"
                     /locus_tag="ACGE4U_002882"
     CDS             4583..6061
                     /gene="msr(C)"
                     /locus_tag="ACGE4U_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFF8394946.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            6108..6554
                     /locus_tag="ACGE4U_002883"
     CDS             6108..6554
                     /locus_tag="ACGE4U_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFF8394947.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6753..7268)
                     /locus_tag="ACGE4U_002884"
     CDS             complement(6753..7268)
                     /locus_tag="ACGE4U_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8394948.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT         2441 a         1297 c         1498 g         2142 t
ORIGIN      
        1 tttatacaga tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag
       61 tgatgttcgt tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg
      121 tcaacctcat cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa
      181 agccaatcca catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg
      241 taacgattgg cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag
      301 acaataccat cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt
      361 gaacagtaag atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg
      421 agaatagaat gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa
      481 tcggtgaagc aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac
      541 gccgagaaga tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc
      601 aaacagcaga tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa
      661 agttcggtcg aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag
      721 ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa
      781 atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa
      841 ctgattctgt ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat
      901 ttgctgtccg tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat
      961 ctacgatcat ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa
     1021 aggctgctct tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag
     1081 cttctgctgt agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta
     1141 tccgtccgac gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg
     1201 ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat
     1261 ttacgtcttt ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata
     1321 tggacattga cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat
     1381 tcaaaggaga aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa
     1441 tctagcagtt gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca
     1501 gacatcgagt tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc
     1561 tactgtctca gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac
     1621 acaaaccgaa gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat
     1681 cacgaaccaa ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga
     1741 attcgaaaac tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc
     1801 gttcattgat agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc
     1861 atcagtcatg attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc
     1921 tgcccctaat tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat
     1981 gcctacttcg gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata
     2041 tccttcgtac aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca
     2101 gccaggcgtt tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc
     2161 tacggcttgg ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa
     2221 tgtaatcgaa acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat
     2281 gtatcgtctg tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa
     2341 aaattatctg ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag
     2401 cggacaacct gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc
     2461 acaatctgag atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta
     2521 ttctggtggt tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca
     2581 tcactatacc ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga
     2641 aggaatagga ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta
     2701 gtaagaacag ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg
     2761 cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa
     2821 cttttttatt actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca
     2881 aaaggcgatt tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc
     2941 tatagtttga taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt
     3001 ccgttcattc tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta
     3061 ttacacagct gattctttga cttttttctt tttttattct attattgaca agtcaatcgt
     3121 tgacgagtca attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt
     3181 atatcaaatc aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat
     3241 aagtcttact agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag
     3301 cagtttgcaa gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt
     3361 ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt
     3421 agcgctaaca gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac
     3481 aggttccgtg ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt
     3541 agataaattg aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa
     3601 tggaacaaaa aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg
     3661 atgaaacata caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg
     3721 caccttcttc tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa
     3781 aagaaatctt gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa
     3841 tggtcatgat ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag
     3901 cttacgaatc agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc
     3961 cttattacaa gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct
     4021 tagcggctat gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg
     4081 gtggattcga agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc
     4141 cagttattat tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag
     4201 caccagaaaa gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg
     4261 ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc
     4321 gatgaaatta cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa
     4381 acaacaattt catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag
     4441 ctatgacaga tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat
     4501 gcagtttttt tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac
     4561 atgaaatagg agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt
     4621 cagctttggc aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg
     4681 tatagggatc ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg
     4741 tgaacttttt cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca
     4801 attaacctat ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt
     4861 aagactggaa gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt
     4921 gatacgtctt ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct
     4981 agatagaaaa agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt
     5041 ttttgttagt catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa
     5101 agacggagaa atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt
     5161 ggagaaaaag actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga
     5221 aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa
     5281 gaaaaagcaa caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca
     5341 aaaagccatc caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac
     5401 aaccaagccg cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca
     5461 cagccgctat cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact
     5521 ggtaaatggt gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg
     5581 ttcaggtaag acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc
     5641 tccgaaagta agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc
     5701 catcattcgt tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct
     5761 gaatcactta gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg
     5821 agaagcgacc cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt
     5881 agatgaaccg actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca
     5941 aatatatccg ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc
     6001 tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta
     6061 aatgtgctac gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata
     6121 aaaaagaagg aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg
     6181 cgtataattt atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc
     6241 ctggattcgt ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa
     6301 ttgcctttaa actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat
     6361 gcttgatggt tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag
     6421 gaatacctga tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg
     6481 ctttaccgtt gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc
     6541 aaagtaatga atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat
     6601 agtctcttga atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt
     6661 tcggatcgct ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca
     6721 atagatattt ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct
     6781 cttacttgct gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca
     6841 acataacgtc caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt
     6901 ttttgcatga ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt
     6961 ttatcgagcg tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg
     7021 ggagtttcct ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag
     7081 atcttttttt gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac
     7141 ttggcaagcg ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc
     7201 ttcaattcat acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa
     7261 gtattcataa aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc
     7321 acgttttaca tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagag
//
LOCUS       DAXWLF010000380         7307 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.guided.131, whole genome shotgun sequence.
ACCESSION   DAXWLF010000380 DAXWLF010000000
VERSION     DAXWLF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.guided.131"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            111..194
                     /locus_tag="ACGE4U_002885"
     CDS             111..194
                     /locus_tag="ACGE4U_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFF8394949.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACGE4U_002886"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACGE4U_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFF8394950.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1226..1486
                     /locus_tag="ACGE4U_002887"
                     /pseudo
     CDS             1226..1486
                     /locus_tag="ACGE4U_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1649..2314
                     /locus_tag="ACGE4U_002888"
                     /pseudo
     CDS             1649..2314
                     /locus_tag="ACGE4U_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2350..2595
                     /locus_tag="ACGE4U_002889"
     CDS             2350..2595
                     /locus_tag="ACGE4U_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8394951.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2698..3567
                     /locus_tag="ACGE4U_002890"
     CDS             2698..3567
                     /locus_tag="ACGE4U_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8394952.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3548..4282
                     /locus_tag="ACGE4U_002891"
     CDS             3548..4282
                     /locus_tag="ACGE4U_002891"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8394953.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4315..5223
                     /locus_tag="ACGE4U_002892"
     CDS             4315..5223
                     /locus_tag="ACGE4U_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFF8394954.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5220..5762
                     /gene="sat4"
                     /locus_tag="ACGE4U_002893"
     CDS             5220..5762
                     /gene="sat4"
                     /locus_tag="ACGE4U_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFF8394955.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5855..6649
                     /locus_tag="ACGE4U_002894"
                     /note="aph(3')-IIIa"
     CDS             5855..6649
                     /locus_tag="ACGE4U_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFF8394956.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6925..7269
                     /locus_tag="ACGE4U_002895"
                     /pseudo
     CDS             6925..7269
                     /locus_tag="ACGE4U_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         2557 a         1100 c         1525 g         2125 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacca ttttgtgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat
      301 aacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 attcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt
     1081 tggaaagtta cacgttacta aagggaatgg agataaatta ttagatatac tactgacagc
     1141 ttccaagaag ctaaagaggt ccctagcgcc tacggggaat ttgtatcgat aaggggtaca
     1201 aattcccact aagcgctcgg gaccccttgt aggaaaatgt cctaagtgtg gcaacaatat
     1261 tgtattaaaa aaatcgtttt atggttgttc aaattatcct gaatgtaagt ttactttagc
     1321 tgaacatttt agaaagaaaa aactaaccaa aacgaatgta aaagaattac tggagggaaa
     1381 agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag ccctacaatg ccgttgtaaa
     1441 aattggggaa aagggatata ttgattttat ctctttttca aaataaacat ggggctgacc
     1501 caaaagtagc tcctatatga aaaatagagg tctttccatt tagatagtag tggaaagacc
     1561 tctatttatc ttttgaaagt gaatacaaaa ataaagcaca ctctgctata atataggtga
     1621 ttaatccatt tgagagaggt gctttatcat gtatcaaaat tataccataa accaactttg
     1681 tttgcctata gatcttgaaa ttaaattaga agaaaatgat tttgctcatg ccattgtgca
     1741 gtttgttgac tctattcccg atgaagtctt tcttccttat tatcaatcta tgggtagacc
     1801 ccaatatcat cctcgtatgc tactttccat tattctttgt gcctatattc aaggggttta
     1861 ttcgggccgc caaatacaaa atatgctgat agatagtatt cggatgcgct acttatctca
     1921 agagcaattc cctaactttc gtactattaa tcgttttcgc gttcatccga ttatgaacga
     1981 tattctagat cattcatttg ttcaatttcg tgagctactg gttcaaagtg gcttaatcag
     2041 tggcagtgct ctatacattg atggtacaaa aattgaagcg gatgcgaata aatactcttt
     2101 cgtttggaaa aaaagtattt taaagtataa ggatagcctt gatcaaaaag cccttgaaaa
     2161 ctaccaacaa atggtggaaa caaaaatctt acctgagtta atagatgatt taaaggatga
     2221 actttctata gaagagattg aaaggattcg tcaatctttg gaagaaaagg aaaatcaatt
     2281 aattgaagcg attgaccaaa ccgaaacagt agaagggggc tgacccatta ttaataagga
     2341 gtggacaaca tgattgatgt aaatgagcta ttggaagaag ctattagaga aactgaaaat
     2401 ctaaatgatg gcgaagtttt tcttgttaaa gatttattca agggatatgt ggggaatagg
     2461 atacccagaa aggatcgact tctgcttgga acgttatttt taaaccatgt tagcaaaatg
     2521 aatggtaatt taaaggctat tgaaaagact tcatctaatc agcagaggta taaaaagaca
     2581 attgataagt tttaactatc ttttgtgaaa ttaaaaaaat tataaatatg ttgagtagaa
     2641 gcaaaagatt tggcttggaa tttttacagc agtgaatcga taaaaggagc atgcttgatg
     2701 gtagataata ttattaaatc agtagcagag aaattatcct ctctgtctta tatagaaggt
     2761 attgttttag gtggttcacg tgcaaggggc acccatacag aggattcgga tatagatatc
     2821 ggcatctatt acaattcaga atcatttgac ataaatacta ttaatcaatt cgctacaaag
     2881 ctggatgatg agcatagaaa taaccttgtt gtacctcccg gagcatgggg tgattggatt
     2941 aatggcggcg gatggttagt cataaacggg tatcatgtgg atttaatttt acgtgatatt
     3001 aaacgtgtgg aacaaataat gaaagataca gagcacggaa ttgttactgc caattatcag
     3061 actgggcatc cccatggtta tattagtgca atgtatcgag gagaattagc gattagcaaa
     3121 atactatatg ctaagaatga aagcttatgc gaattaaaaa aacaggcaga aacttatccc
     3181 aatgctttgc agaaaagttt agttaacttt tttatgtttg aagcagggtt ctctttaatg
     3241 tttgtaaaag caaattcggg aacagacgat aaatattata ttgcgggtca tgtttttcgt
     3301 atagtttcat gtttaaatca agtgttattt gcatgtaata atgcttattg tatcaacgaa
     3361 aagaaagcta taaaactgct tgaaactttt gaacataaac ctgaaaaata taccgagaag
     3421 gtaaatcata tttttgaagt actcggtatc tcactttttg aatgctacga catgaccgag
     3481 aagctttata atgaagtgaa tgaaattgta tcggagataa ataacttttt aaacgaggag
     3541 agttcagatg aaagaaaaca aatatgatga taatatattt tttcaaaaat acagtcaaat
     3601 gagtcgctcg cagaaaggac tggctggtgc gggagaatgg gagactttga aaaagatgct
     3661 acctgatttt aagggtaagc gtgtgcttga tttaggatgc ggctatggat ggcactgtat
     3721 atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt gatatttctc ataaaatgct
     3781 cgaagtagca aaaggaaaaa cccattttcc acagattgaa tatgaatgct gtgccataga
     3841 agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg cgtttcatta
     3901 tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg ctggtggcaa
     3961 tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag actggtatta
     4021 taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg agggcaaacg
     4081 gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga ccacatatct
     4141 aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac agccgccaga
     4201 gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga tgctgattgt
     4261 atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat gtaaatgaga
     4321 tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga acgtattcga
     4381 attgtgaccc ttgaggggtc acgcgcaaat attaatatac ctaaagatga atttcaggat
     4441 tatgatatta catattttgt aagtgatata gaaccgttta tatctaatga tgactggctt
     4501 aatcaatttg ggaatataat aatgatgcaa aagccggagg atatggaatt attcccacct
     4561 gaagaaaagg gattttccta tcttatgcta tttgatgatt acaataaaat tgatcttacc
     4621 ttattgccct tggaagagtt agataattac ctaaagggcg ataaattaat aaaggttcta
     4681 attgataaag attgtagaat taaaagggac atagttccga ctgatataga ttatcatgta
     4741 agaaagccaa gcgcaaggga gtatgatgat tgctgcaatg aattttggaa tgtaacacct
     4801 tatgttatta aaggattgtg ccgtaaggaa attttatttg ctattgatca ttttaatcag
     4861 attgttcgcc atgagctgct gagaatgata tcatggaagg tcggcatcga aacaggcttt
     4921 aaattaagtg taggcaagaa ctataagttt attgaaaggt atatatccga ggatttgtgg
     4981 gagaaacttt tgtccaccta ccggatggat tcctatgaaa acatatggga agcattattt
     5041 ctatgccatc aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct tcattatgcc
     5101 tatccggagt atgataggaa tataacaaaa tataccaggg acatgtataa aaaatacact
     5161 ggtaaaaccg gctgcctgga tagcacatat gccgctgata tagaagagag gcgggaacag
     5221 tgattacaga aatgaaagca gggcacctga aagatatcga taaacccagc gaaccatttg
     5281 aggtgatagg taagattata ccgaggtatg aaaacgagaa ttggaccttt acagaattac
     5341 tctatgaagc gccatattta aaaagctacc aagacgaaga ggatgaagag gatgaggagg
     5401 cagattgcct tgaatatatt gacaatactg ataagataat atatctttac taccaagacg
     5461 ataaatgcgt cggaaaagtt aaactgcgaa aaaattggaa ccggtacgct tatatagaag
     5521 atatcgccgt atgtaaggat ttcagggggc aaggcatagg cagcgcgctt atcaatatat
     5581 ctatagaatg ggcaaagcat aaaaacttgc atggactaat gcttgaaacc caggacaata
     5641 accttatagc ttgtaaattc tatcataatt gtggtttcaa aatcggctcc gtcgatacta
     5701 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt
     5761 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct
     5821 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga
     5881 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
     5941 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
     6001 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
     6061 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
     6121 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
     6181 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
     6241 ttgtccctat acgaatagct tagacagccg attagccgaa ttggattact tactgaataa
     6301 cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg
     6361 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
     6421 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
     6481 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag
     6541 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     6601 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt acctagattt
     6661 agatgtctaa aaagctttaa ctacaagctt tttagacatc taatcttttc tgaagtacat
     6721 ccgcaactgt ccatactctg atgttttata tcttttctaa aagttcgcta gatagagttc
     6781 tatattagag atgtaaagag ttttggtgaa gaggtttgtg agtatggctt ttataaccag
     6841 ggtgcaatga gaagcactaa cagcagctag cttaagaacg cataggatat actttttatg
     6901 gaagtcacag aggaggaata aaatttgaac aggattaaca gagtaacctc tattcttatt
     6961 cagctgcaat caaaaaaaat aattcctgcc aaagaaattg cacagcgatt taatataagc
     7021 ttaaggacag tttacagaga tatccggaca cttgaagaag ccggaatacc aattggatct
     7081 gaggccggaa aaggatattt tcttgtagag ggcttcctac ttccgccggt aatgtttaca
     7141 gcggcggaag tgggtgcatt gattacagca gggaaatttt taaattgcca tggagatgaa
     7201 tcatttataa aggattttga ttcagctatg tataaaatca aatctatttt gaagcatggt
     7261 gaaaaaaacg gttctgttgc aaagttttaa ataaagaata aaatccc
//
LOCUS       DAXWLF010000381         6009 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.guided.151, whole genome shotgun sequence.
ACCESSION   DAXWLF010000381 DAXWLF010000000
VERSION     DAXWLF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.guided.151"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            241..1209
                     /locus_tag="ACGE4U_002896"
     CDS             241..1209
                     /locus_tag="ACGE4U_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFF8394957.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1202..2233
                     /gene="vanA"
                     /locus_tag="ACGE4U_002897"
     CDS             1202..2233
                     /gene="vanA"
                     /locus_tag="ACGE4U_002897"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFF8394958.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2239..2847
                     /locus_tag="ACGE4U_002898"
     CDS             2239..2847
                     /locus_tag="ACGE4U_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFF8394959.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3275..4186
                     /gene="vanY"
                     /locus_tag="ACGE4U_002899"
     CDS             3275..4186
                     /gene="vanY"
                     /locus_tag="ACGE4U_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFF8394960.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4339..4824
                     /gene="vanZ-A"
                     /locus_tag="ACGE4U_002900"
     CDS             4339..4824
                     /gene="vanZ-A"
                     /locus_tag="ACGE4U_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFF8394961.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5111..5440)
                     /locus_tag="ACGE4U_002901"
     CDS             complement(5111..5440)
                     /locus_tag="ACGE4U_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFF8394962.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5745..>6009
                     /locus_tag="ACGE4U_002902"
     CDS             5745..>6009
                     /locus_tag="ACGE4U_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8394963.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIA"
BASE COUNT         1943 a         1013 c         1268 g         1785 t
ORIGIN      
        1 tggtgtcatc caccaacatc aaaaagtata gagccataat tttttaggaa aatctcaagg
       61 ttatctttac tttttcttag gaaattaaca atttaatatt aagaaacggc tcgttcttac
      121 acggtagact taataccgta agaacgagcc gttttcgttc ttcagagaaa gatttgacaa
      181 gattaccatt ggcatccccg ttttatttgg tgcctttcac agaaagggtt ggtcttaatt
      241 atgaataaca tcggcattac tgtttatgga tgtgagcagg atgaggcaga tgcattccat
      301 gctctttcgc ctcgctttgg cgttatggca acgataatta acgccaacgt gtcggaatcc
      361 aacgccaaat ccgcgccttt caatcaatgt atcagtgtgg gacataaatc agagatttcc
      421 gcctctattc ttcttgcgct gaagagagcc ggtgtgaaat atatttctac ccgaagcatc
      481 ggctgcaatc atatagatac aactgctgct aagagaatgg gcatcactgt cgacaatgtg
      541 gcgtactcgc cggatagcgt tgccgattat actatgatgc taattcttat ggcagtacgc
      601 aacgtaaaat cgattgtgcg ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt
      661 ggcaaggtac tcagcgacat gacagttggt gtggtgggaa cgggccagat aggcaaagcg
      721 gttattgagc ggctgcgagg atttggatgt aaagtgttgg cttatagtcg cagccgaagt
      781 atagaggtaa actatgtacc gtttgatgag ttgctgcaaa atagcgatat cgttacgctt
      841 catgtgccgc tcaatacgga tacgcactat attatcagcc acgaacaaat acagagaatg
      901 aagcaaggag catttcttat caatactggg cgcggtccac ttgtagatac ctatgagttg
      961 gttaaagcat tagaaaacgg gaaactgggc ggtgccgcat tggatgtatt ggaaggagag
     1021 gaagagtttt tctactctga ttgcacccaa aaaccaattg ataatcaatt tttacttaaa
     1081 cttcaaagaa tgcctaacgt gataatcaca ccgcatacgg cctattatac cgagcaagcg
     1141 ttgcgtgata ccgttgaaaa aaccattaaa aactgtttgg attttgaaag gagacaggag
     1201 catgaataga ataaaagttg caatactgtt tgggggttgc tcagaggagc atgacgtatc
     1261 ggtaaaatct gcaatagaga tagccgctaa cattaataaa gaaaaatacg agccgttata
     1321 cattggaatt acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga
     1381 aaacgacaat tgctattcag ctgtactctc gccggataaa aaaatgcacg gattacttgt
     1441 taaaaagaac catgaatatg aaatcaacca tgttgatgta gcattttcag ctttgcatgg
     1501 caagtcaggt gaagatggat ccatacaagg tctgtttgaa ttgtccggta tcccttttgt
     1561 aggctgcgat attcaaagct cagcaatttg tatggacaaa tcgttgacat acatcgttgc
     1621 gaaaaatgct gggatagcta ctcccgcctt ttgggttatt aataaagatg ataggccggt
     1681 ggcagctacg tttacctatc ctgtttttgt taagccggcg cgttcaggct catccttcgg
     1741 tgtgaaaaaa gtcaatagcg cggacgaatt ggactacgca attgaatcgg caagacaata
     1801 tgacagcaaa atcttaattg agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt
     1861 gggaaacagt gccgcgttag ctgttggcga ggtggaccaa atcaggctgc agtacggaat
     1921 ctttcgtatt catcaggaag tcgagccgga aaaaggctct gaaaacgcag ttataaccgt
     1981 tcccgcagac ctttcagcag aggagcgagg acggatacag gaaacggcaa aaaaaatata
     2041 taaagcgctc ggctgtagag gtctagcccg tgtggatatg tttttacaag ataacggccg
     2101 cattgtactg aacgaagtca atactctgcc cggtttcacg tcatacagtc gttatccccg
     2161 tatgatggcc gctgcaggta ttgcacttcc cgaactgatt gaccgcttga tcgtattagc
     2221 gttaaagggg tgataagcat ggaaatagga tttacttttt tagatgaaat agtacacggt
     2281 gttcgttggg acgctaaata tgccacttgg gataatttca ccggaaaacc ggttgacggt
     2341 tatgaagtaa atcgcattgt agggacatac gagttggctg aatcgctttt gaaggcaaaa
     2401 gaactggctg ctacccaagg gtacggattg cttctatggg acggttaccg tcctaatcgt
     2461 gctgtaaact gttttatgca atgggctgca cagccggaaa ataacctgac aaaggaaagt
     2521 tattatccca atattgaccg aactgagatg atttcaaaag gatacgtggc ttcaaaatca
     2581 agccatagcc gcggcagtgc cattgatctt acgctttatc gattagacac gggtgagctt
     2641 gtaccaatgg ggagccgatt tgattttatg gatgaacgct ctcatcatgc ggcaaatgga
     2701 atatcatgca atgaagcgca aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg
     2761 tttgaagcat atagcctcga atggtggcac tatgtattaa gagacgaacc ataccccaat
     2821 agctattttg atttccccgt taaataaact tttaaccgtt gcacggacaa actatataag
     2881 ctaactcttt cggcaggaaa cccgacgtat gtaactggtt cttagggaat ttatatatag
     2941 tagatagtat tgaagatgta aggcagagcg atattgcggt cattatctgc gtgcgctgcg
     3001 gcaagatagc ctgataataa gactgatcgc atagaggggt ggtatttcac accgcccatt
     3061 gtcaacaggc agttcagcct cgttaaattc agcatgggta tcacttatga aaattcatct
     3121 acattggtga taatagtaaa tccagtaggg cgaaataatt gactgtaatt tacggggcaa
     3181 aacggcacaa tctcaaacga gattgtgccg tttaagggga agattctaga aatatttcat
     3241 acttccaact atatagttaa ggaggagact gaaaatgaag aagttgtttt ttttattgtt
     3301 attgttattc ttaatatact taggttatga ctacgttaat gaagcactgt tttctcagga
     3361 aaaagtcgaa tttcaaaatt atgatcaaaa tcccaaagaa catttagaaa atagtgggac
     3421 ttctgaaaat acccaagaga aaacaattac agaagaacag gtttatcaag gaaatctgct
     3481 attaatcaat agtaaatatc ctgttcgcca agaaagtgtg aagtcagata tcgtgaattt
     3541 atctaaacat gacgaattaa taaatggata cgggttgctt gatagtaata tttatatgtc
     3601 aaaagaaata gcacaaaaat tttcagagat ggtcaatgat gctgtaaagg gtggcgttag
     3661 tcattttatt attaatagtg gctatcgaga ctttgatgag caaagtgtgc tttaccaaga
     3721 aatgggggct gagtatgcct taccagcagg ttatagtgag cataattcag gtttatcact
     3781 agatgtagga tcaagcttga cgaaaatgga acgagcccct gaaggaaagt ggatagaaga
     3841 aaatgcttgg aaatacgggt tcattttacg ttatccagag gacaaaacag agttaacagg
     3901 aattcaatat gaactatggc atattcgcta tgttggttta ccacatagtg cgattatgaa
     3961 agaaaagaat ttcgttctcg aggaatatat ggattaccta aaagaagaaa aaaccatttc
     4021 tgttagtgta aatggggaaa aatatgagat cttttattat cctgttacta aaaataccac
     4081 cattcatgtg ccgactaatc ttcgttatga gatatcagga aacaatatag acggtgtaat
     4141 tgtgacagtg tttcccggat caacacatac taattcaagg aggtaaggat ggcggaatga
     4201 aaccaacgaa attaatgaac agcattattg tactagcact tttggggtaa cgttagcttt
     4261 ttaatttaaa acccacgtta actaggacat tgctatacta atgatacaac ttaaacaaaa
     4321 gaattagagg aaattatatt gggaaaaata ttatctagag gattgctagc tttatattta
     4381 gtgacactaa tctggttagt gttattcaaa ttacaataca atattttatc agtatttaat
     4441 tatcatcaaa gaagtcttaa cttgactcca tttactgcta ctgggaattt cagagagatg
     4501 atagataatg ttataatctt tattccattt ggcttgcttt tgaatgtcaa ttttaaagaa
     4561 atcggatttt tacctaagtt tgcttttgta ctggttttaa gtcttacttt tgaaataatt
     4621 caatttatct tcgctattgg agcgacagac ataacagatg taattacaaa tactgttgga
     4681 ggctttcttg gactgaaatt atatggttta agcaataagc atatgaatca aaaaaaatta
     4741 gacagagtta ttatttttgt aggtatactt ttgctcgtat tattgctcgt ttaccgtacc
     4801 catttaagaa taaattacgt gtaagatgtc taaatcaagc aatctgatct ttcatacaca
     4861 taaagatatt gaatgaattg gattagatgg aaaacgggat gtggggaaac tcgcccgtag
     4921 gtgtgaagtg aggggaaaac cggtgataaa gtaaaaagct tacctaacac tatagtaaca
     4981 aagaaagccc aattatcaat tttagtgctg aggaattggt ctctttaata aatttcctta
     5041 acgttgtaaa tccgcatttt cctgacggta cccccaaact aagatttcat agatagattt
     5101 tctgataact ttaaaaaaca cgaatagcgg aaaggatgtc ttcattcgct aaagagtaat
     5161 aaactacttt tccatcttga cgtgattttg ccattccatg tttttttaaa aaacgtaaat
     5221 ggtgtgacgt tgtagcgaca ctagcagtaa gtatttctgc taaatcacag acgcacattt
     5281 ccttataggt ttctaaagca tagaaaattt taattctaga aaaatcgctg aaacatttcc
     5341 ctaacacaag taagtttgaa aaatcttttt gatccaattt attttttatt ccatttactt
     5401 tttctttatg tacgacagtc acttggcaaa cttttttcat tcatctcacc tcacattcaa
     5461 acgattattt gaatgaagta tacctatttg atcactcttt tgtcaatcta ccttaattcc
     5521 aatacactat tataagcaat ttcatgtatt gaagtaaact tacttgcaag tgcgtttttg
     5581 attccaagtt aaaaacatat tattattcta cataatttcc taatcaaata aaactccttt
     5641 gaaaattgtt ctaaaggagt tttattattt tgtatttcta ctaaaaatag cgtacaatgt
     5701 aattactaag taattacgaa atgtaaaata atggaggaaa taatatgatt acaaaaacaa
     5761 gaaaacaagg aaattctatt atgttaacgg tccctaaaga attcgatgtg cccaatggtg
     5821 ttgaagtaga agctaagctt gtagagaatg gtatccttta tgagtttgta gagcctaaaa
     5881 aagaattttt tgattttagt gaagatattt tggctgatat tctttcagaa ggttatagca
     5941 aacaagagat cttaaaagaa tttagaaata gaaaaaatgc gttaacttct gcatttaaat
     6001 ctattgctg
//
LOCUS       DAXWLF010000382         4083 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.guided.196, whole genome shotgun sequence.
ACCESSION   DAXWLF010000382 DAXWLF010000000
VERSION     DAXWLF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.guided.196"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            complement(<1..437)
                     /locus_tag="ACGE4U_002903"
     CDS             complement(<1..437)
                     /locus_tag="ACGE4U_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8394964.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDG"
     gene            563..1684
                     /locus_tag="ACGE4U_002904"
     CDS             563..1684
                     /locus_tag="ACGE4U_002904"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFF8394965.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1942..3444
                     /gene="eat(A)"
                     /locus_tag="ACGE4U_002905"
     CDS             1942..3444
                     /gene="eat(A)"
                     /locus_tag="ACGE4U_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFF8394966.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3511..4038
                     /locus_tag="ACGE4U_002906"
     CDS             3511..4038
                     /locus_tag="ACGE4U_002906"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8394967.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1360 a          728 c          891 g         1104 t
ORIGIN      
        1 ccatcaccta acaatgtgac ttttccggga ccagctagac cagctgtcca catcgtgatc
       61 ccaaataaga tatttttctt tggcacatat ttttccagta catcttcatg acctaatcca
      121 tttagtaaac acaaaacata agtattgtca gtaataatgg attggatcga acaaaacata
      181 tcatctaatt gattggcttt tgttaatgct acgatcaagt ccacgtgttc gttgctttcg
      241 atgatttctt ctggtgaata gattggcagt tttgcgacca cttctttacc gttaaagtca
      301 gcaatcaacc catttgtacg aatggcttca atgtgagcag gccaacgatc gatcaagagg
      361 acctcatttc cgctttgatg tagcatcaat ccgattcggc tgcccattgc tccggcccca
      421 gctattgcga ttttcatgtt ttttctctcc tttattcaat gagttccctt ttagtatata
      481 gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa tgaaaaaaag tgtcttactt
      541 aagaagaaag tgagggatac agatggatat tttggcggca attgattcat ttaaaggaag
      601 tgcttcttca tttgaactaa atcaggcagt tctaaatggc tgggaagatc gtaaaggaag
      661 aaaaatcaat gtgccgattg cagatggcgg agaaaatact gcagaagcaa tctattacgc
      721 gctcagggga acatggcgta ccattgatgg gatggatctc ttattccgtc agcgaaaaat
      781 cagctattta ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat ctgcacaagc
      841 attggggtta gatctacttg ataaaccaac ggatcaaact atccgacttg cttccagtta
      901 tgccttagga gaacttatta aagatgcact gcttcaagaa gtagaacaaa ttattgtcac
      961 tttaggagga agcgggtgta gcgatggtgg tctaggatta ctccaatcct tgggtgcttc
     1021 tttagaaggg tacacagaag gaaatccttt attatctact aaaaaacttc attttgatcg
     1081 aagaaacgtt cttgtttcta aagtaaaatt gattatcgct gcagatgtta ccaatccata
     1141 tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa tagatacgac
     1201 gctagctttc ttagataaac aagcaggaaa gattgctcag caaataaaag atttctggga
     1261 ggtggattta gatgatattc caggaagtgg agcagcaggc ggtattggtg gagccctttt
     1321 tttgttggga gcgaagatgg tttctggttt cgatttagtc agccaaatca ctggattgga
     1381 aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg atcagcaaac
     1441 gatccacgga aaagttccat acggtgtagc agtgcttgca aaaaaatatc gaaaaccaat
     1501 aatcgctatt tgcggaagta gagaccaaga gctagaagga ctttctgatt ttctcccaat
     1561 tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac acaggcaaac
     1621 attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca tcctcccgaa
     1681 gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga caaagtgagg
     1741 gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact gtaccttgtg
     1801 gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga tgtatgggtc
     1861 tttttgtgtg tatccaaccc ttgcatcctc tttagccagt tctgaaaata ggactggtct
     1921 ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac
     1981 gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa
     2041 ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac
     2101 aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag
     2161 acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata
     2221 tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa
     2281 ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat
     2341 gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa
     2401 caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg
     2461 ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt
     2521 tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg
     2581 gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa
     2641 gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat
     2701 actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa
     2761 cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta
     2821 aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga
     2881 cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat
     2941 tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca
     3001 tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga aaaataccag
     3061 ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg aggaacgttg
     3121 gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag
     3181 cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg
     3241 aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac atgggatgaa
     3301 ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg
     3361 aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag
     3421 attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag
     3481 gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca
     3541 acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga
     3601 cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa
     3661 atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc
     3721 agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga
     3781 ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca
     3841 aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc
     3901 gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag agtgatcaat
     3961 gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa
     4021 ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg
     4081 ctt
//
LOCUS       DAXWLF010000383         2748 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.guided.225, whole genome shotgun sequence.
ACCESSION   DAXWLF010000383 DAXWLF010000000
VERSION     DAXWLF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.guided.225"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..339
                     /locus_tag="ACGE4U_002907"
     CDS             <1..339
                     /locus_tag="ACGE4U_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8394968.1"
                     /translation="KKSDYKPKAIESEGTVDSITTNEINEFLTTFFKLYPTATASELS
                     YYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEYIDQQTKATQVSQFDLVLE
                     KNGSNWKIIE"
     gene            653..700
                     /locus_tag="ACGE4U_002908"
                     /pseudo
     CDS             653..700
                     /locus_tag="ACGE4U_002908"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            716..2635
                     /gene="tet(M)"
                     /locus_tag="ACGE4U_002909"
     CDS             716..2635
                     /gene="tet(M)"
                     /locus_tag="ACGE4U_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFF8394969.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          942 a          422 c          557 g          827 t
ORIGIN      
        1 aagaaatcag actataaacc aaaagctatt gaaagtgagg gtacggttga ttccattaca
       61 accaatgaaa tcaatgagtt tttaacgacg ttcttcaagc tctatcctac agcgacagcc
      121 agtgaacttt cctactatgt gaatgacggg atattaaaac caatcggaaa agagtacatc
      181 tttcaagaac tggtaaatcc tatttacaat cgtaaggata atcaagtcac ggtatcgctg
      241 acagtggagt atatcgacca gcagaccaaa gcaacgcagg tatctcaatt tgatttggta
      301 cttgaaaaga acgggagtaa ttggaagatt atagaataac aaatattggt acattattac
      361 agctattttg taatcacgta ctctctttga taaaaaattg gagattcctt tacaaatatg
      421 ctcttacgtg ctattattta agtatctatt taaaaggagt taataaatat gcggcaaggt
      481 attcttaaat aaactgtcaa tttgatagtg ggaacaaata attggatgtc cttttttagg
      541 agggcttagt tttttgcacc cagtttaaga atacctttat catgtgattc taaagtatcc
      601 ggagaatatc tgtatgcttt gtatgcctat ggttatgcat aaaaatccca gtgataagag
      661 tatttatcac tgggattttt atgccctttt gggcttttga atggaggaaa atcacatgaa
      721 aattattaat attggagttt tagctcatgt tgatgcggga aaaactacct taacagaaag
      781 cttattatat aacagtggag cgattacaga attaggaagc gtggacaaag gtacaacgag
      841 gacggataat acgcttttag aacgtcagag aggaattaca attcagacag gaataacctc
      901 ttttcagtgg gaaaatacga aggtgaacat catagacacg ccaggacata tggatttctt
      961 agcagaagta tatcgttcat tatcagtttt agatggggca attctactga tttctgcaaa
     1021 agatggcgta caagcacaaa ctcgtatatt atttcatgca cttaggaaaa tggggattcc
     1081 cacaatcttt tttatcaata agattgacca aaatggaatt gatttatcaa cggtttatca
     1141 ggatattaaa gagaaacttt ctgccgaaat tgtaatcaaa cagaaggtag aactgtatcc
     1201 taatatgtgt gtgacgaact ttaccgaatc tgaacaatgg gatacggtaa tagagggaaa
     1261 cgatgacctt ttagagaaat atatgtccgg taaatcatta gaagcattgg aactcgaaca
     1321 agaggaaagc ataagatttc agaattgctc cttgtaccct gtttatcatg gaagcgcaaa
     1381 aagcaacata gggattgagc agcttataga agtgataacg aataaatttt attcatcaac
     1441 atacagaaag aagtctgaac tttgcggaaa tgtcttcaaa attgaatatt cggaagaaag
     1501 acaacgtctt gcatatgtac gcctttatgg cggaatcctg catttgcggg attcggttag
     1561 aatatcggaa aaggaaaaaa taaaaattac agaaatgtat acttcaataa atggtgaatt
     1621 atgtaaaatt gataaggctt attccgggga aattgttatt ttgcaaaatg agtttttgaa
     1681 gctaaatagt gttcttggag atacaaagct attgccacag agagagagaa ttgaaaatcc
     1741 gctccctctg ctgcaaacaa ctgttgaacc gagcaaacct caacaaaggg aaatgttact
     1801 tgatgcactt ttagaaatct ccgacagtga cccgcttcta caatattatg tggattctac
     1861 gacacatgaa attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact
     1921 gttgcaagaa aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga
     1981 gagaccgtta aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg
     2041 ggcttccatt ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag
     2101 ctcggtttct cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg
     2161 ctatggttgc gaacaaggat tatatggttg gaatgtgacg gattgtaaaa tctgttttaa
     2221 gtatggctta tactatagcc ctgttagtac cccagcagat tttcggatgc ttgctcctat
     2281 tgtattggaa caagtcttaa aaaaagctgg aacagaattg ttagagccat atcttagttt
     2341 taaaatttat gcgccacagg aatatctttc acgagcatac aacgatgctc ctaaatattg
     2401 tgcgaacatc gtagacactc aattgaaaaa taatgaggtc attcttagtg gagaaatccc
     2461 tgctcggtgt attcaagaat atcgtagtga tttaactttc tttacaaatg gacgtagtgt
     2521 ttgtttaaca gagttaaaag ggtaccatgt tactaccggt gaacctgttt gccagccccg
     2581 tcgtccaaat agtcggatag ataaagtacg atatatgttc aataaaataa cttagtgtat
     2641 tttatgttgt tatataaata tggtttcttg ttaaataaga tgaaatattt tttaataaag
     2701 atttgaatta aagtgtaaag gaggaattaa ttatgaagtg taatgaat
//
LOCUS       DAXWLF010000384         2555 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.guided.228, whole genome shotgun sequence.
ACCESSION   DAXWLF010000384 DAXWLF010000000
VERSION     DAXWLF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.guided.228"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            <1..459
                     /locus_tag="ACGE4U_002910"
     CDS             <1..459
                     /locus_tag="ACGE4U_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8394970.1"
                     /translation="TLFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELIDNIRD
                     KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFK
                     GRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            673..1368
                     /gene="vanR-A"
                     /locus_tag="ACGE4U_002911"
     CDS             673..1368
                     /gene="vanR-A"
                     /locus_tag="ACGE4U_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFF8394971.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1346..2500
                     /locus_tag="ACGE4U_002912"
     CDS             1346..2500
                     /locus_tag="ACGE4U_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFF8394972.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          881 a          454 c          535 g          685 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc tacaggaaga tgacatcatt
       61 tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga attaatcgat
      121 aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct tgatttatca
      181 gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa ccaattagag
      241 cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa agaaggaaag
      301 tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta tgcggtaaag
      361 ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa tgtatctagg
      421 gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat tccgctaatg
      481 ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc tagcgatgcc
      541 gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc atttattatg
      601 taaatgctta taaattcggc cctataatct gataaattat taagggcaaa cttatgtgaa
      661 agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga aattgccgat
      721 ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta taccgccaaa
      781 gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga catcatgctt
      841 cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac ctatccgatt
      901 atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac aatcggcgcg
      961 gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt aaaggcccag
     1021 ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt tatcgtccac
     1081 tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa gcagttatcc
     1141 cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg gaatgtggtt
     1201 agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa gagcaacaac
     1261 accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat tgataatccg
     1321 aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa aaaacgacta
     1381 ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg tagcaattgt
     1441 attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga tcttaagtat
     1501 tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc aatattccat
     1561 acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc ttattctatg
     1621 tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg gcattgatgt
     1681 acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg ttatggaaca
     1741 aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa agctggccga
     1801 acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc cccttacatc
     1861 cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag atcaaaaggc
     1921 aaagtatgtg catatcacgt tggacaaagc gtatcgactc gaacagctaa tcgacgagtt
     1981 ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc acatagacct
     2041 atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg cacatggaaa
     2101 acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc
     2161 gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata acagcatcat
     2221 tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga acactggaag
     2281 catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg acaatgctcg
     2341 ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa ttattgttca
     2401 gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta gggtagagct
     2461 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata taattttggc
     2521 tctttgtcaa atgatgttgg taaagagaaa tctta
//
LOCUS       DAXWLF010000385         1068 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.guided.307, whole genome shotgun sequence.
ACCESSION   DAXWLF010000385 DAXWLF010000000
VERSION     DAXWLF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.guided.307"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            24..>1068
                     /gene="tet"
                     /locus_tag="ACGE4U_002913"
     CDS             24..>1068
                     /gene="tet"
                     /locus_tag="ACGE4U_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HFF8394973.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTELGRSRPSSI"
BASE COUNT          304 a          161 c          220 g          383 t
ORIGIN      
        1 aaagtgtaaa ggaggaatta attatgaagt gtaatgaatg taacagggtt aaatacaacc
       61 aaattttaat ttggctttgc gttttatctt tttttagcgt attaaatgaa atggttttga
      121 acgtctcatt acctgatatt gcaaatgatt ttaataaacc acctgcgagt acaaactggg
      181 tgaacacagc ctttatgtta accttttcca ttggaacagc tgtatatgga aagctatctg
      241 atcaattagg catcaaaagg ttactcctat ttggaattat aataaattgt ttcgggtcgg
      301 taattgggtt tgttggccat tctttctttt ccttacttat tatggctcgt tttattcaag
      361 gggctggtgc agctgcattt ccagcactcg taatggttgt agttgcgcgc tatattccaa
      421 aggaaaatag gggtaaagca tttggtctta ttggatcgat agtagccatg ggagaaggag
      481 tcggtccagc gattggtgga atgatagccc attatattca ttggtcctat cttctactca
      541 ttcctatgat aacaattatc actgttccgt ttcttatgaa attattaaag aaagaagtaa
      601 ggataaaagg tcattttgat atcaaaggaa ttatactaat gtctgtaggc attgtatttt
      661 ttatgttgtt tacaacatca tatagcattt cttttcttat cgttagcgtg ctgtcattcc
      721 tgatatttgt aaaacatatc aagaaagtaa cagatccttt tgttgatccc ggattaggga
      781 aaaatatacc ttttatgatt ggagttcttt gtgggggaat tatatttgga acagtagcag
      841 ggtttgtctc tatggttcct tatatgatga aagatgttca ccagctaagt actgccgaaa
      901 tcggaagtgt aattattttc cctggaacaa tgagtgtcat tattttcggc tacattggtg
      961 ggatacttgt tgatagaaga ggtcctttat acgtgttaaa catcggagtt acatttcttt
     1021 ctgttagctt tttaacagaa ttaggcagaa gtcgcccatc ctctatag
//
LOCUS       DAXWLF010000386          962 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.guided.315, whole genome shotgun sequence.
ACCESSION   DAXWLF010000386 DAXWLF010000000
VERSION     DAXWLF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.guided.315"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            349..897
                     /gene="aac(6')"
                     /locus_tag="ACGE4U_002914"
     CDS             349..897
                     /gene="aac(6')"
                     /locus_tag="ACGE4U_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFF8394974.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          340 a          139 c          191 g          292 t
ORIGIN      
        1 tcttagtatt tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt
       61 aagattatta tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca
      121 aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct
      181 gatctttcct attttgaata aagtgtttct gtataatttt tttattttat aagtaagata
      241 aataatgaaa gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg
      301 ttttttcaaa aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt
      361 gaatttgacc gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact
      421 tggccggaag aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa
      481 cgaatcgcgg tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct
      541 caatacggta tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag
      601 aaccaaatag gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga
      661 atcacgattt atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac
      721 ctgtatgagc atacatttga taaagtggct tccatccaga accttcgtga acatccgtat
      781 gaattctatg aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg
      841 gacaaaccgg atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa
      901 aaagaggttg tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac
      961 tt
//
LOCUS       DAXWLF010000387          604 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id56
            SAMN40749880-rid21412723.guided.344, whole genome shotgun sequence.
ACCESSION   DAXWLF010000387 DAXWLF010000000
VERSION     DAXWLF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749880
            Sequence Read Archive: SRR28555116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134726.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134726.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:51:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,771
            CDSs (with protein)               :: 2,771
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749880-rid21412723.guided.344"
                     /isolate="id56"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-08-10"
     gene            62..559
                     /gene="dfrG"
                     /locus_tag="ACGE4U_002915"
     CDS             62..559
                     /gene="dfrG"
                     /locus_tag="ACGE4U_002915"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFF8394975.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          233 a           61 c          131 g          179 t
ORIGIN      
        1 aaggtttttt gatagaaata agaaattaga tggtaaatta gttaaaaagg ctgtgttata
       61 aatgaaagtt tctttgattg ctgcgatgga taagaataga gtgattggca aagagaatga
      121 cattccttgg aggattccca aggactggga atatgttaaa aatactacaa agggacatcc
      181 gataatatta ggtaggaaga accttgaatc aatcggaaga gccttacctg acagaagaaa
      241 tattattctg acgagagata aggggtttac ctttaatggt tgtgaaattg ttcattcaat
      301 agaagatgtt tttgagttat gtaaaaacga agaagaaatt tttattttcg gaggagaaca
      361 gatttataat ttgtttttcc cttatgttga gaaaatgtac atcacaaaaa tacatcatga
      421 attcgaagga gatacttttt ttccagaagt gaattatgag gaatggaatg aggtatttgc
      481 ccaaaaaggg ataaagaatg ataaaaatcc gtataactac tattttcatg tatatgaaag
      541 aaaaaactta ttgagttaac gggtgcgtta attgaagaag aacagtaaag ataaagcaca
      601 agag
//
